LOCUS       DAOOAB010000001       140114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_69_length_140114_cov_16.382018, whole genome shotgun sequence.
ACCESSION   DAOOAB010000001 DAOOAB010000000
VERSION     DAOOAB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 140114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 140114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..140114
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_140114_cov_16.382018"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1807
                     /locus_tag="PKU88_00005"
     CDS             425..1807
                     /locus_tag="PKU88_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPX67711.1"
                     /translation="MKYDVIIVGAGPSGIFAAYELMQYDAGIKVAILEKGNELEKRAC
                     PIDNKKIKSCINCPTCSIMNGFGGAGGMSDGKYNITNNFGGELYKYIGRKEALDLMYY
                     VDEVNCKMGGEGAMLYSTENSSLRTQALRHDLHLLDAKVRHMGTDKNLVIMKNLYEYL
                     MDKVEIIFNNEVSDIEKQGDTFIVSAEKGEYSCSHLILAAGRSGSKWISQICEKLGIK
                     TESNRVDIGVRVEIPASIFEHITSQVYESKIVYRTEKYNDLVRTFCMNPHGKVVTENT
                     NGIVTVNGHSYSDPKHHTENTNFALLVSNRFTEPFKNSNEYGESIAKLSNMLGGGVLV
                     QRFGDLVKGRRSSERRMEKSFLRPTLNATPGDLSLVIPKRQLDDIIEMIYALDKIAPG
                     TANEDTLLYGVEVKFYNSQVEVDNNLETIIKGLYILGDSSGVTHSLSQASASGVYVAR
                     LLGEKYCKHS"
     gene            1840..3117
                     /locus_tag="PKU88_00010"
     CDS             1840..3117
                     /locus_tag="PKU88_00010"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPX67712.1"
                     /translation="MSSLVIVGAQWGDEGKGKLTDYLAEKADVVVRYQGGNNAGHTVE
                     VDNEVYKLQLIPSGILYADKMCIIGNGVVIDPEAFLNEIRNLESRGISTGNLRISDRA
                     HVLFPYHKKIDELSELKRGSDDLGTTKKGIGPCYADKTERIGIRVCDLLKKEVLAKKL
                     KTNLELKNAYLKKIYDFEGYSYEEMLSKYLDYAEQVRPFVEDTSVLIYDAIKAGKKVL
                     FEGAQGTLLDLDFGTYPYVTSSHPIAGGVCIGAGVGPALIGKVIGVVKAYTTRVGKGP
                     FPTELFDETGEYIRTTGKEFGTVTGRPRRCGWFDAVIVRYAARISGLTGIAITKLDTL
                     SNIPTIKMCVGYRLDGKIIRDFPASLEDLENCEPIYEEFEGWGDIGDITQYNKLPTSV
                     LRYLERIEEICGTKISIISVGPKRNQTIMIDKF"
     gene            3227..3302
                     /locus_tag="PKU88_00015"
     tRNA            3227..3302
                     /locus_tag="PKU88_00015"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3260..3262,aa:Lys,seq:ttt)
     gene            3308..3382
                     /locus_tag="PKU88_00020"
     tRNA            3308..3382
                     /locus_tag="PKU88_00020"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3341..3343,aa:Glu,seq:ttc)
     gene            3389..3464
                     /locus_tag="PKU88_00025"
     tRNA            3389..3464
                     /locus_tag="PKU88_00025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3422..3424,aa:Val,seq:tac)
     gene            3609..3685
                     /locus_tag="PKU88_00030"
     tRNA            3609..3685
                     /locus_tag="PKU88_00030"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3643..3645,aa:Asp,seq:gtc)
     gene            3689..3764
                     /locus_tag="PKU88_00035"
     tRNA            3689..3764
                     /locus_tag="PKU88_00035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3722..3724,aa:Thr,seq:tgt)
     gene            3922..4200
                     /locus_tag="PKU88_00040"
     CDS             3922..4200
                     /locus_tag="PKU88_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPX67713.1"
                     /translation="MPFKALSDPSRRKIIQMLKGKDMTAGEIADNFQMSKPSISHHLS
                     ILKQADLVTDERRGQNIYYSLNTTVFQEVVNWFFNMTDRSGGFENEKD"
     gene            4187..4840
                     /locus_tag="PKU88_00045"
     CDS             4187..4840
                     /locus_tag="PKU88_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HPX67714.1"
                     /translation="MKKIDLNIKKELPIILIIVLMFAASIYFYPQLPDKIPTHWNFKG
                     EIDGYSGKLTGAFLMPVMNIIMYGLFIFIPAIDPKKENYKLFESTYIYFRYLFHIFFL
                     GIHGLIIASALGYNVDTGRLVTLAVSLLLMLMGNVMGRLKHNYFVGIKTPWTLANEEV
                     WRKTHRLGAILWTAGGLAGVIMSLLRLSMGWAFIAVIAVMTFIPIVYSYVIYSKFKK"
     gene            4986..5960
                     /locus_tag="PKU88_00050"
     CDS             4986..5960
                     /locus_tag="PKU88_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPX67715.1"
                     /translation="MKIVVLDGYTLNPGDLSWDGLEKLGEITVYDRVSYDEKDEDMVA
                     ESVGDAEIVFTNKTPVTRRAMKKMPNLRFIGVLATGYNIVDVVAAKEKGVVVTNIPTY
                     GTDSVGQFAIALLLEMCHHVGAHSDSVLRGEWANNPDWCYWNFPLIELAGKTMGIIGY
                     GRIGQTTGRIAQALGMRVIAADSYKNPALESDTMKYVELDELFAKSDVIALHCPLFES
                     TKGIINRENIAKMKDGVMIINNSRGPLIVEEDLAEALNSGKVAGAAVDVVMTEPIKPD
                     NPLLKAKNCIITPHISWAPKESRKRLMDIAVNNLEKFLAGTPVNVVNP"
     gene            6549..7664
                     /locus_tag="PKU88_00055"
     CDS             6549..7664
                     /locus_tag="PKU88_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPX67716.1"
                     /translation="MDKLKPYNDLTPISQLAIEENKPLLKLAEDTRVAIEILNYEINS
                     LPSPSIMIDTLTLQEAKVSSHIENIVTTNDDLYKGIVFDNYTAEAKEVTNYKTALFIG
                     FERLKEKGVLSIGDIEAINEPVNKKTKGIRVNMPEFENSLTRIANKRGNEIEVLYTPP
                     HGKELLQKLLIDMLEYLYDDETYTIHPLIKIALAHYQFECIHPFYDGNGRTGRILNVL
                     MLCQKRYLDYPILFASSYIIKNKNEYYELLQTCRQKDNYTSIIEYMLNSFKVTAEDTL
                     NKVNSIKKLIEKYSDQEFLETLKGQKKVLSQVMELIFKKVYVRIEDIVEMGIHRQTAS
                     AYLKQLVEKGLLQEEKVQRDKVFKNIELLKLFEGEQI"
     gene            7661..9385
                     /locus_tag="PKU88_00060"
     CDS             7661..9385
                     /locus_tag="PKU88_00060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPX67717.1"
                     /translation="MINQADINAKVNLVTKIANKLRGPYTSEQYGSVIIPMSILRRFD
                     CVLEGQNSKIKLILEKFKSLNVDEKLIEQAVQKECGIPFYNKKCVTFEDLISDSDNIN
                     ANFMEFLTSYSPSIANIIKGLKFKEEVEFMIKKGILFEVVKAFSEVDFHPDTTDQLSM
                     GYIFEEIIRTYKSNAEAGDHYTPREVIELCVELMVAENIDELKKEGKIIRVYDGCCGT
                     GGMLTVTKRKLGELLGSDERVRLFGQDNNPEAYAICCAEMLMLGEDVKNIYEGNTLTE
                     DHLKGEKFDILITNPPFGVEWKTEKAIVEKELNSPNNRFVAGTPRVSDGQLLFLQNLL
                     AKAEKNARIAIILNGSPLFSGDAESGESEIRRYVLENNLLEAIVALPDQMFYNTGIFS
                     YIWILSRNKSEKRDKKVQLINAVDFWEPQQPKSLGNKRKFISPHNRKKIVELYSKFEE
                     NEYSRKFDNIDFGYHKVTIERPLRDETGAPIIKKGKVQADGALRDTENIPLKEDINEY
                     FNREVKPYAPDAWIDHDKIKVGYEIPFTRYFYKYVPPRKSGDILSEIIEIEDDIKGTI
                     ETLLGRRE"
     gene            9385..10656
                     /locus_tag="PKU88_00065"
     CDS             9385..10656
                     /locus_tag="PKU88_00065"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPX67718.1"
                     /translation="MHNSQCIIYNGKISINEVKTKYLFKIVNGATPSSSNIEYWDGNI
                     TWITPADMSKCISNIIIDSDRKITEEGLNSCGTSLVPAGSIILSTRAPIGSVCIAGVE
                     LCTNQGCKALVAKDNVNERFYFYYLLISAELLNSLGRGTTFLELSGSDLGNFLLPFPK
                     KAKQDEIANYLDQKCAEIDRVVAAKQKQNELLKEYKQSIIYEAVTKGLDKNVKYKDSG
                     VEWIGEIPEDWEKWKIKYVCDVDKNRLSEDTDPNYLFKYIDISSVTEMGGIGETVEMT
                     FDTSPSRARMIVSKGDTIISTVRTYLKAIAFIEESNKYICSTGFAVLTSKKGLLPRFL
                     FYQAQSEYFIQEVVSRSVGVSYPAITSSALSNISFFVPSLPTQQRIADYLDQKCCEID
                     RIIKSNDDMITKLKEYRQSVIYEAVTGKIEM"
     gene            10672..13605
                     /locus_tag="PKU88_00070"
     CDS             10672..13605
                     /locus_tag="PKU88_00070"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPX67719.1"
                     /translation="MAGEVLRENPFRDEIYNYLLSIGYKESIKADYDYMNAIDRAKLF
                     EFLENSQKEQLESFKDTYKDSWMERFIKLLNDKINNEGLLAALKGKLEDYQSSTGFSL
                     AFFGSNIESMTYNKELYEKNIFSACREFAYEDKQDSFRVDIAIFLNGIPIIMIELKKQ
                     TTGQKAGFEGTSQFRNTRNPDELIFGFNKRTLVYFAVDEFEAFAATRLNRKNTVFVPF
                     NRGSSDEGAGNPIVEGKHCTNYVWEEILRKNMLLKIIREFMFIDEDGEMIFPRYHQLD
                     AVLKLEADVKQNGIGGRYLIWHSAGSGKTKTIAWLSKRLINIQDIHTVIVISDRTVID
                     SQLGIEIQTLDGKKGVAKWIDTTSAELLKMLRSGGYIVITTLQKFGNILDELKNIPNR
                     NYAIIIDEAHSSTGGKSLSKTAETLDGKSLKDSILIDETYEELEDNQNRILKQGSKIK
                     STKNISYFAFTATPKTETVELFGTRTAIGKKYFHKYSMKQAIQEGFILNPIVCYTIYN
                     EKFELAKKKDDPTEYETAKASAAILNHITSSAEVIDSKTEIIMEDFLIKRINWLDGKA
                     KAMIITPSRKHAVCYKLAVDKYLIKKGYNIKTIAAFTGTIELNGIKYTEENMNPGFKE
                     KDIKEIIKNNDDVRVIIVADKLQTGFDEKKLCILYVDKKLNSAVKAVQTLSRINRICK
                     GKKTFILDFVNKTEEIKAYFEQYYGGELFLPSENETDPNILFTKRDRILNYFVFTIEQ
                     AEYAYSLINDNARHSGELTSIFAKAKEKYNEMDKDRKKLFLAEISKFVKLFYYVSIVY
                     NIWNSDMKRFAVFLDALYNVLYEKSDDTRINPGELVELVEYSVKKASEEEDLLVNVAD
                     YSLERVPTESYVKEESYSLIDEIIYRINARYGKYENAHQELVEIVNTLSSDNEMIINV
                     RDSSPSAYEAEAMDKIGQIFVNGILSAAEERSQFYAQISSDKDVIRSLAKAVIRRIQD
                     ELKAG"
     gene            complement(13663..14643)
                     /locus_tag="PKU88_00075"
     CDS             complement(13663..14643)
                     /locus_tag="PKU88_00075"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX67720.1"
                     /translation="MNNNQIIQKQYELIRKENMEEIEARKKTLYDKLPRLKEIEDEMV
                     KLSIDITRAILSKSNTAEVLIDKLKNKQLDLKVEKAEILSANKYPIDYLDSIYKCKLC
                     KDTGFVNYQKCKCYKQREIYLNYEYSNLSFILEKENFEQFRWDYYSDDPDENGISPLK
                     NMQEIYKKCIDFVEQFDEHNKNLFFIGSPGLGKTFLCNSIAKDLLDKGRSVIYMTVPD
                     LIDTMRKYKFDFDKEENYAHYLNEIYNCDLLIMDDLGTELSTQFSNQAIYNILNKRIV
                     NMKKMIISTNLSGVDFQSTYSERIVSRIIGNFEACKFVGKDIRLIMKGIS"
     gene            complement(14636..15661)
                     /locus_tag="PKU88_00080"
     CDS             complement(14636..15661)
                     /locus_tag="PKU88_00080"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HPX67721.1"
                     /translation="MNLFRIKSNNEDLGQTIVENLFISHYMPFAPADYVKVYLLGLKY
                     SQSYVNNMLSTDTVAKTLGITRDEVHAAWRYWSEQDIIKLYPYSPDDAESGFTVEYLS
                     IKEVMLNIKEEKPSAGKYSPERIINARGNQDVRAMFDSFRKLFGRELSPNELFVFLDW
                     MDDYNFPPDVIKMLVEECVSRDRKEMPYLKQVAKNWFDAGIDSVDKAKQYNSRYKEKW
                     QKYNKVLSFLRIGRQPTSAEEEMLQKWFYTFEYSEDIVLKACELTASTLKPSFSYIDR
                     ILTEWHGKGLSTMPEVEAYLVKNPSKAGRNSKPEKRTFNNFANRTYDAKALEEAWLKK
                     SRGELSE"
     gene            15951..17435
                     /locus_tag="PKU88_00085"
     CDS             15951..17435
                     /locus_tag="PKU88_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPX67722.1"
                     /translation="MEGSKLKEAAARYWNKISGGIENIKKHIKNIIKKIANNGANSLN
                     KHTYSRKIVNKRRFAIACGLIALLVFASIFVYASGKAYTVKVNGVEICKVKNPKTVES
                     ALNILKQKYQEKADSEISFTSEVTYEKTRASSKEILKDDALVEVLGKNVEFKVQGCSL
                     YADGNIIAILKTKEKAEELLKEIQDRSLAGADRSKLKEIGFAEKVELRDEFIDASEIA
                     DKDEIIDFIIKGTNEIRIHEVKSGESFWSISRKYNMSLLDLEKANPGVNPERIQIGQK
                     INLIVPKPLISVKTVETITFIEKAEFDQKVEFSSSLYKDETSIRVKGIYGEKEVVADV
                     TKVNGIETGRTILSEKMLKEPKTQIIVKGTKEPPPKKGTGTFSYPARGSITSRYGMRW
                     GRRHTGIDIGAPYGSDVKASDGGEVTFVGYQGGYGKLIIIDHGANFVSYYGHLSKYSV
                     KVGQKVYKGQKIGEVGSSGNSTGPHVHFEIRKNGVIQNPANYLK"
     gene            complement(17471..18694)
                     /locus_tag="PKU88_00090"
     CDS             complement(17471..18694)
                     /locus_tag="PKU88_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPX67723.1"
                     /translation="MISISLCLKSFKKVDPAVKRYISIKKVKTIPVIITFKRPLDPKT
                     ESALKRKGLRIKYHIPFLNAVTGKISIGNFDSVSSFVEIAKIYFDGTAKLMGGTESGD
                     KALESPEAASVHLSGKGITAAFIDSGVYPHPDLTRPRNRITAFKDYINEINEPYDDNG
                     HGTACIGAAFGASIDGKFKAAAYDCNIVCAKAFNSLGYGCFSDILAAMQWIYSIKEKY
                     NIKVLVLPFGCCCTSRHFDILSLASEALWNNGLFICTCAGNLGPHEASITSPGICGSA
                     FTTGACTVSGSSSRVAPFSGRGPVDGKFDKPDAVMPGYKIATLNADTDYIPGSKSSYQ
                     SKLSGQQYTQLSGTSAAASMTAAAAVLLYQKKPNLPPSDIKSLLKRFCISLNELKTAQ
                     GAGIIEIKKIGEFKF"
     gene            18808..19017
                     /locus_tag="PKU88_00095"
     CDS             18808..19017
                     /locus_tag="PKU88_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPX67724.1"
                     /translation="MALIDFKCIKCGEKFEEIVKLSDRENIKCPKCGSEDIKQVFEGK
                     CCSVGSSKGVGGCSSGSCGGCSGCH"
     gene            19128..19961
                     /locus_tag="PKU88_00100"
     CDS             19128..19961
                     /locus_tag="PKU88_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67725.1"
                     /translation="MKKRISKPAIVLILAAVLFFFTGDKGFMAVKMQGYEALKVFAHI
                     KSSEFTPEFNIYETEHFIIKYTDKDEDTVRDIAGIFEKSYKVEGIHYGFYSKDKTIVF
                     VYNDQESMWDYQQSVKGQAVMGLYNLGIIHVLSPKAYLDSMQIYAKDYEADGPILHEY
                     VHRVIDYRSGGNVELWLTEGLALYEEYDVDGTEWAKGFSYERHYTAHELRDDFMELDE
                     TQSYRQSFDIVWSLIEQRGRNSINLMLDELKYGSTLDEAFIRIYGEDLNEYIDSGAWK
                     R"
     gene            20412..21110
                     /locus_tag="PKU88_00105"
     CDS             20412..21110
                     /locus_tag="PKU88_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX67726.1"
                     /translation="MENRRILVVDDERPISDIIKFNLEKEGYKVFVAEDGQQAVNMTY
                     EHKPDLIILDIMLPIMDGFTVCKKLRENVSTPIIMLTAKEEEVDKVLGLELGADDYMT
                     KPFSVRELMARVKANIRRANFLNSGLEGSGQVVKSGNLIIDLNKYEVRKDDTVLELTL
                     REFELLRYLALHAEQVFSRETLLEEVWGYEYYGDVRTVDVTVRRLREKIEDDPSNAVY
                     VQTKRGVGYYFRRI"
     gene            21114..22886
                     /locus_tag="PKU88_00110"
     CDS             21114..22886
                     /locus_tag="PKU88_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX67727.1"
                     /translation="MFKSIQWKLVIISFLLVWLAMSIVGVFITEAIKKEQIDSMTDTI
                     ITRGYYLADNLKDKIGGASNIQEIVSNWFVGQGKQVRAVFVYENGGFTASQTNYEGAV
                     DDLDLLGLWIVEEAIDQAQEITWPVDNSKYLMSIAVPIISSNNDVAGAIYISADLFGI
                     EENIMSINKILTSATILALCITALLGNLLSRTFTGPIKEVTSKAERLAKGDFGQTIAV
                     KSRDEVGQLTEMFNYLTVRLKSTLDEMSREKNKVEAILTNMTDGIIAVNAEGVVIHAN
                     PAAYKIFNIREEDLYNRNFDDAAEKLDLGITFNDILNDSDNNYNILSINNLIIKISVV
                     QIINEHNKAEGAMLVLQDVTEQEKLDKMRKEFVANVSHELRTPLTTIRSYTETLLDGA
                     LDNKEYTLNFLKVINSESERMTRLVKDLLQLSKLDYDKMEWNMKSLSILNILRDCVVK
                     MEMAAKQKNQSLSFEAIGELCEINGDKDRIEQVIINIISNAIKYTPENGSIKVTAKRL
                     KDSVEIRIADSGIGIPKDDLPRLFERFYRVDKARSRSMGGTGLGLSIAKNIVEAHKGS
                     IRIESEYGKGSEVIINFPCEESLL"
     gene            23005..23526
                     /locus_tag="PKU88_00115"
     CDS             23005..23526
                     /locus_tag="PKU88_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67728.1"
                     /translation="MFRKVLITAIIVTAVFAAGSAVYAQSGTDYAEETAPVFKLLSDY
                     ESKTFDRDYMITGSASEGTVVTIELYWFKSEKSIIVNKKQSASSESEGDWIFQDSKEY
                     TVGSSGIFAEPVALNLGKNKIVLLISNADGETEEITLELVRSLEKDAKEEVNGPSLNK
                     FVEDLSSKVNSTE"
     gene            23537..24961
                     /gene="yycH"
                     /locus_tag="PKU88_00120"
     CDS             23537..24961
                     /gene="yycH"
                     /locus_tag="PKU88_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HPX67729.1"
                     /translation="MIILNRERIKSVSLLFLVILSFVLTTRIWFYTSIEGVFIMSGNK
                     SIKPDYNAEYDKSDLLRPHKLIVSFGERSTLLYDNEEYSEAYGRIFKEAKRVMRTAIL
                     VNSKEVVKKIPMEELYKIRNARGITFIYNLPLEMEAVYKLMNINSYPDTGIRSIDEII
                     VAQSLDRAYLCDSVQGILYEFRTEGIQNNLDYLISNLEKQPTVLSLYLDSIQPEMYGK
                     NSAVPARIGANGFPVLSGQKEVEGSDEIPESIAGLFDDEITSLSIIKNMDGTIVYTNR
                     EGQVVKLYTDGMLEYVNFNLQPEDKTTVGLGGAIDISTEFVSRHFGFPEDCYISDIIR
                     INSMGDDKYIIRYRYAYEGLPVLSASDISSGSIEVEIVGDEVRRYKRIRRTLNHNFEY
                     KQAMNSINVLDILLDKKVEALSGERIKKVNDMYLAYYERFGQNSITYVPVWVAEVTVE
                     RIDKGTVLNQKYIINAETGIILDK"
     gene            25019..25897
                     /gene="yycI"
                     /locus_tag="PKU88_00125"
     CDS             25019..25897
                     /gene="yycI"
                     /locus_tag="PKU88_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HPX67730.1"
                     /translation="MDWAKAKTILIVVFTAINIFLGYMVFAAGTGGFGYVDSEKAKQI
                     TDYLAEKDVTVTGRIPGKKADMPSIIVKYKLFSKEDVKGSFFSTGDVPEESASENTVG
                     LKGKNIEITIRNNREFYYSDNGIKPAGATDEKACRKNIREFLSRLNITDNLDTGISED
                     IEGYKRFLFSQSFKGAEIYNSIMEFYVNDSGVHRARIVWFDTIRQAGKKTEVISPLIA
                     LLYVPEHNKKSELPGREVVEIRQGYYFGTGAGEQVDVSEVVEGTAFPVWKIETDRDII
                     YINAYNEKVEGIEKRQ"
     gene            25994..27253
                     /locus_tag="PKU88_00130"
     CDS             25994..27253
                     /locus_tag="PKU88_00130"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPX67731.1"
                     /translation="MEEKLIIKGNKKLQGEVCISGAKNSAVGIIPAALLSDSGCIIKN
                     LPDIEDVRYLKKLLQQVGAKVNDGNTGELEVISKGMKNYRVNFEIAKKMRASYYFLGA
                     FLGKFKKAEVPFPGGCDIGIRPIDQHIKGLEALGAKIKVEHGIIKAKASELVGAPIYL
                     DVVSVGATINIMLAACKAKGVTTIENAAKEPYIVDIANLLNAMGANIKGAGTDVIKIK
                     GVEHLGSCEHMIIPDMMEAGTYMIAAAATQGDLLVKNVIPTHLESVSAKLREMGIEVD
                     ENGDSIRVTTVARPKAVNIKTIPYPGFPTDLQQPMSVLLSISEGTGIITESIWEGRFK
                     HVDELKRMGANIKVEGRVAIIEGINKLSGSPVHATDLRAGAAMVIAGLTAEGETEIDN
                     LKHIDRGYENMVEKLRNAGADITRVQC"
     gene            27281..28078
                     /locus_tag="PKU88_00135"
     CDS             27281..28078
                     /locus_tag="PKU88_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX67732.1"
                     /translation="MGIRLCSLSSGSSGNCIFVGNDRSGLLVDCGISGKETLKNLHSI
                     GVCSSTIKAVVVTHEHSDHIKSVGIISRKLGIPIYANINTWNSMRALIGTVKPENIRS
                     FKTGEELEIAGIGIKSFNIPHDAADPVGFSFYEGNNKVSIATDLGYFSDTVRENIRGS
                     DMILLESNHDIEMLMTGRYPFFLKRRIMSERGHLSNEAAGNAVNELLQTGVREVLLGH
                     LSQENNFPELAYETVKGILKQNNINVDADIKLDLAPRNGISRFYNID"
     gene            28160..29374
                     /locus_tag="PKU88_00140"
     CDS             28160..29374
                     /locus_tag="PKU88_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPX67733.1"
                     /translation="MDNFDENSYPEIQGNKTAEENNTSAPTAYRNEPIRYKRPSGFLF
                     LTTALVGAVIGGLIVAAIVPSYLYDRMLSVQQGGIAPSGQQLVIQPSNEMNVAAAVAQ
                     KVTPAVVRISTVTVERDLFFGKRSYEGVGSGVIIDPKGYILTNAHVVGEHPEELTVFF
                     KDGKELDGTVLWQDSLLDLAVVKVEATGLPSAELGDSDSLIVGEISIAIGNPLGMRFE
                     RTVTQGIVSGLNRSIMVEQGSVMEDLIQTDAAINPGNSGGPLINGRGQVIGINTVKAS
                     AEGLGFAIPINITKPIIRKLIDTGEFIPTYMGITPLDSEMLGYYAINIEIQKGIYIYN
                     IMKGAPAASAGLEAGDVITHINGTEVNTLVKFKSLLYSLNPGDTVSVRYLRSNTEYNS
                     DIKLAEMPEEYR"
     gene            29374..29853
                     /gene="rlmH"
                     /locus_tag="PKU88_00145"
     CDS             29374..29853
                     /gene="rlmH"
                     /locus_tag="PKU88_00145"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPX67734.1"
                     /translation="MNISIITVGKLKERYLKEAVEEYSTRLSKYCKLDIMEVPDEKAP
                     ENLSPAQELTVMQKEGQGILRHIKEDTYVIALAIQGRQISSEGLADFINDLGIKGRSN
                     IAFIIGSSLGLSEEVMKRADFKLSFSPMTFPHQLMRVMLLEQVYRGFRIIKGEPYHK"
     gene            30072..30644
                     /locus_tag="PKU88_00150"
     CDS             30072..30644
                     /locus_tag="PKU88_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX67735.1"
                     /translation="MVTRKEQKYKRRNEILEAGLDLFIRRGYASTKISDIANQVGMST
                     GLLFHYFESKEKLYVELIKNGLAGPMSIMTDSGMEPIVFFKTTADWILGSLKTDTVFA
                     KYFVLMSQAYYNESAPQSVKDMLKGFDIYTPTSHLIKKGQANGSIKEGEPYALAIAYW
                     CAIQGIAEQMAMTPEYPCPEGQWIVDILRR"
     gene            30648..32159
                     /locus_tag="PKU88_00155"
     CDS             30648..32159
                     /locus_tag="PKU88_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPX67736.1"
                     /translation="MKKIIIVGAGIAGLSAGIYARQSGMDVTIFESHTIPGGASTSWR
                     RKGYLFEGGMHWLTGSSPKVPLNKVWRELGALNDDVPVYNKDPFLTVEFEGRTAFLYR
                     DVEKLRQHLLELAPEDRNEIDKLCRDIKRFSKVSMPIRDIKNVKVKKKTSVPLSDFIK
                     MLPAFSRMSFYANQTAGEFAMRFKSPILRALIRCIIGEEYSAIGLIFTIATLASGDGG
                     YPEGGSLAMASRMAKYFESLGGKINYKSRVTQVSVKDGISDGVIINGEHFPADAIIVT
                     QDTLVAIDSLFDPPINEPWAYKMRQSIKPTLNTFICLGIEADLSDLPETISFMPHKPL
                     LCGGAEVEFISINNYSGYEGYAPNGCTAVTSIISGDTYDYWKACREHGTYEEEKRKLA
                     ENFIEILAERFPEIREKVALWDVATPLTYERYLSSYKGSWMSITGKGEKMNGYPSKSE
                     KIQNVYFAGQRLSPPGGLPVAVETGRKAVQYLCRDTGTIFQANINDGMVPGTY"
     gene            32131..32586
                     /locus_tag="PKU88_00160"
     CDS             32131..32586
                     /locus_tag="PKU88_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPX67737.1"
                     /translation="MTVWCPALIRRRKDYTGGYDKDGNLTDDPNAIFDGGEVLPSGYW
                     RGSGFSIVIDMVVSALSVGKSTYDMGMNKNDKGVSQVFIAINPIAYAGENHADAYIEN
                     MSQYIKTSAKKAGVTDILYPGEKAARERIENMRENIPVNKEMWETLLKL"
     gene            32682..33326
                     /locus_tag="PKU88_00165"
                     /pseudo
     CDS             32682..33326
                     /locus_tag="PKU88_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            33405..33980
                     /gene="msrA"
                     /locus_tag="PKU88_00170"
     CDS             33405..33980
                     /gene="msrA"
                     /locus_tag="PKU88_00170"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPX67738.1"
                     /translation="MATAHITGTDKKLQTAIFAGGCFWCMVSPFDVLEGIIEVKSGYS
                     GGHKENPTYKDVKSQTSGHYEVIKIIYDPEIIGYDKLLQAYWRQVDPTDDGGQFQDRG
                     ESYRTAIFYTNEEQRQMAEASRKAMDESGRFPGPIVTPIIPAAVFYEAEEYHQDFYKK
                     SPKEYKEDRKISGRDEFIKKCWGDEYYDIYK"
     gene            34080..35255
                     /locus_tag="PKU88_00175"
     CDS             34080..35255
                     /locus_tag="PKU88_00175"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPX67739.1"
                     /translation="MMNKLEIMGRDIRKDLQLFAQELVRIKSTSGNEETIIRFIASKM
                     EALGYDEVIIDGFGNVVGRVGSGEKAVMFDSHVDTVEVKGAELWKVPPFSGEIIDGWL
                     WGRGSVDMKSGAAASIYAAAIAKSQGLLEGKRVYVSCTIFEEDCDGENLKHMFKELQF
                     KPDFFVTCEPSGNIITTGHKGKAQIVIKTQGISAHGSAPEKGVNAIYEMAEIINRVEK
                     TNLELMTKAAPKGTMVMSRISSTGASLNAVPSECEVYLDRRMVVGETEEIIKNEIEKI
                     IKGKNATWEIGTLHRKTWTGMDITYEPFHLAWEISLDHKLTKAFIDSYKEVFGTEPEK
                     YDFWDFSTNAVATVSMGIPTIGFGPGEYKLAHMTNERCEISQIIDSCSIYTNVITKL"
     gene            35298..35858
                     /locus_tag="PKU88_00180"
     CDS             35298..35858
                     /locus_tag="PKU88_00180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX67740.1"
                     /translation="MSVSMFHRLEGRNIYFKPLNIIDAEAIHSYASDENVKRFIGWPL
                     MKSLEETRSYIEDMLKRESAGTHLYASVALKSTREIIGTVMIFNFDREANHAEIGYVF
                     HSKHWGKGHGTESVALMCSFAFEILALHKIHARVVDANISSARILEKNGFLLEGRLKD
                     HYFIDGRYYDSLIYGKFEADEKTLSY"
     gene            35878..36006
                     /locus_tag="PKU88_00185"
     CDS             35878..36006
                     /locus_tag="PKU88_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67741.1"
                     /translation="MAKVVIQECREYELNGLMIKINDGIERLGGWDSFVHPGIRFY"
     gene            35967..36380
                     /locus_tag="PKU88_00190"
     CDS             35967..36380
                     /locus_tag="PKU88_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPX67742.1"
                     /translation="MGFICTPGDKVLLKVNLIGSKSSDSAAVTHSEFVRAVARILKQR
                     GCEVWIGDSSGGAIGGIAPTAKSFRTAGYEKAAMEEGAVKNVFGCIPGLRKARYHKMA
                     PNPESFGEILADIHQNAEFKLPKRYKSGKSETIKY"
     gene            complement(36672..38021)
                     /locus_tag="PKU88_00195"
     CDS             complement(36672..38021)
                     /locus_tag="PKU88_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX67743.1"
                     /translation="MKKARFILPLIIMMMFLFSLDIFAEELLSAEDYYNKGLAFFEQS
                     NYSEAINNYKKAIELDPTYIQAYLDMSDACFYQRKYEEAIANYDAVIELEPANAYAYY
                     SKGVMLYFLFKYDEAIKMYDEAIKIDPDYGDAYQSKGNSLFSLERYSEAIECYDKALK
                     LNPFDADIIEALGDTYYLQELYEKAIEYYDLAIYYNTSDKFVYIYKGCSLYHLSKYDE
                     AIKALELGLEKLPDDAVANYYMSLSLVKTGNTKDYCPYLKKAVSLNKLYLKRAPSEEA
                     FKNLLQDEAFIEIIYDAEEEIHDIFENSDILESDALAEIHFLNMKTSGNAKEDAKALK
                     AERDFLNNFYNGNIYNSASAKKCHISSNSSHITDFIKSGRLIKGQALVGLKITHNISK
                     ENILYIGKQDGVLKAVIFEIAGEIKMDDFSLAINDYIIFIYNNGIWEYFGNLEPKEL"
     gene            complement(38040..39416)
                     /locus_tag="PKU88_00200"
     CDS             complement(38040..39416)
                     /locus_tag="PKU88_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPX67744.1"
                     /translation="MKKDKEVIFFILILVLLLSVSNVYADEEPIIPATPVLTPDITDQ
                     TDKTVMVTISNWDTADTKEYKIDNGPWQEYTEPVALTTNATIYARGSASSGTVSDIAS
                     LKITNIRRLLSKTEVSVQASLTVIIVIYDINNNVVGYGSGFIVSSDGQVVTNYHIIDM
                     APRIDVITTNNETYAVAGVSAYDKKLDLAVLKLEKVKDLPAVILGDSDSLQLGDDIVA
                     IGYPLGLPVTVSFGNVSSLNTPGVLYRTGQKDIQITAPISSGSSGGPLINMYGEVVGI
                     NYSSIENAQNINYSIPINDLKPMLAPASIKKLPDIIKKVYPSMNYDQLAKYLYFNYLD
                     KISGDYTFSFTDIEIRDSGNQPDLVDVYLDLNSLKYAEILLAELEGNKKEVEKWIDEI
                     YSKVKAINPRKNVYVHVALYWSFATRPQGYTNQEAYYNADTKRWVIYKKKVKYGYKNN
                     KPDITWYK"
     gene            39538..39636
                     /locus_tag="PKU88_00205"
                     /pseudo
     CDS             39538..39636
                     /locus_tag="PKU88_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546024.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
     gene            40072..41835
                     /locus_tag="PKU88_00210"
     CDS             40072..41835
                     /locus_tag="PKU88_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX67745.1"
                     /translation="MKEGVTDMAKFNNIKLSNKMSLLLTIPIIAIVITTYISVTNIGT
                     VSEVLVKKLYNEINQSTALMLNADRDFYQALADQMNMQKSTNQEELQRYRESYNENAK
                     QVVDRVHEAYGILLRQKSVYEKFRHQVSNKTVFELIEAFNRDYNRWYGLFNASTNVVS
                     DEEQYINAFNSARESMNQIEEVLEVYGAEIVAQNNKTVLQTQRYVVVITALAVILSLL
                     LGIIIITGISKRTRKTLELIKKTANLDLENSAEYDKFLKEKDEFGLIINAESEVRKEL
                     VNIIKEVSNNASTINKIVDLTNQRMSRLGEEIDGVSATTEELSAGMEETAASVQEMNA
                     ASNEIERASENIAENAKEGSLEAEKISKTANELNKSFTVSQESAYRIFEEVKISLEEA
                     LERSKAAEQISILADSILEITSQTNLLALNAAIEAARAGEAGKGFAVVADEIRKLAEN
                     SKNTVTEIQNITQTVLSSVNDLSSSSKSLLDFMNSDVVKDYGMMLQATKQYKKDAEFV
                     NGLVENLSATAEMLLVSIQSTVKALDEVSAATNEGAEGTSNIAQQTSNIVENAGEVIK
                     NIANAKEVSAKLNQMIAKFKI"
     gene            complement(41989..42285)
                     /locus_tag="PKU88_00215"
     CDS             complement(41989..42285)
                     /locus_tag="PKU88_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPX67746.1"
                     /translation="MGNKVNLWSKIELNKTEIALSLAIKLLLFSGLLGSIIGMISILA
                     SLSNLSKIGPNLAVSFLTMLYSVLFVFILLPVQSKVKAMILSMDKEYIDEKSAN"
     gene            42335..42505
                     /locus_tag="PKU88_00220"
     CDS             42335..42505
                     /locus_tag="PKU88_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67747.1"
                     /translation="MGIVKDRIINMLGRSIKNAMEPEIIAENTKKAMKRTIPAKYINN
                     YLPNKNYVYGMS"
     gene            42715..44112
                     /locus_tag="PKU88_00225"
     CDS             42715..44112
                     /locus_tag="PKU88_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HPX67748.1"
                     /translation="MKSIDTKKLHNSRFVENARIVTVSILLIYLTVSLYFMNHFFFNT
                     VINGADVSLKAYNEADEVIIRYVKDYKLQLIERNEKTEEIAGQDIGMKFNRNNSISKI
                     HKMQTSFKWPISLFKEQSFYVNDLYFYNRENLEDRIDNLDCLNKDITEPQNVSFKYTN
                     GCYETVKEIYGNKLHKGRLYEVIKSSILRGQRELDLDKSLCYINPKYTLSSAETSQTQ
                     SLLNKYVSAKITYILRSEKEILDQNTISQWLSVDEGLEVVIDENAVRRYVKGLSKKYD
                     TAGAARKFKTSVNKTLEVKGGFYGWKTDIAAEAQALTENIKRGEVLEKEPIYIQKALS
                     RDENDIGDTYVEINITRQYLWYYKAGRLIAQGHIVSGNPNRGNATSLGVYMLNYKETD
                     TTIRGPGYEAEVTYWMPFNGNIGIHDADWRYSFGGNIYKRNGTHGCVNVPLYLAKRIY
                     ENIEVGTPVICYEEQ"
     gene            44240..45289
                     /locus_tag="PKU88_00230"
     CDS             44240..45289
                     /locus_tag="PKU88_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPX67749.1"
                     /translation="MRKKCSKTGFIFVLIIMMVALTANITYADSDPSQPSNWAKAEIE
                     KAIEINLLPERLQGEYGSNITREEFSEVAVNLYEAISGKEAELQGENPFTDTQSTEVR
                     IANNLGIVKGIGDGRFAPDNAITRQEISVMMYRTLQAAKPGYDYTKKYEHIFADYDMI
                     STWAREAVSYLYVMEVVNGVGDNRFSPKGNTTREQAVVLAKRMHDKVSASKDNLIASR
                     DGTSRQENDLKLKLAKLISQELGKPYKWGGTGPDGYDCSGLVYSIYGKLGIPLPRTAR
                     DQAGAGIYVSKEALQYGDLVLFARDGKNINHVGIYVGDGMMVHSPQTGDVIKKTTIMS
                     GYYERCYYTARRVIE"
     gene            complement(45320..45778)
                     /locus_tag="PKU88_00235"
     CDS             complement(45320..45778)
                     /locus_tag="PKU88_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPX67750.1"
                     /translation="MIIRKAILPHVEEMYELVNYYAEKGLMLPRSRSALCENIRDFVI
                     IEIDGQVAGIGALHVFWNDLAELRTLAVRSGMERQGIGRRIVEFILKEAKELGLQKVF
                     TLTYQPGFFEKMGFKVVDKETMPQKVWTDCINCPKFPNCNEICLEINIQQ"
     gene            complement(45870..46412)
                     /locus_tag="PKU88_00240"
     CDS             complement(45870..46412)
                     /locus_tag="PKU88_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67751.1"
                     /translation="MSIHYEEVSEDNIICIKDLCNDLMTYQKSKAYIHPEWFDNMSYE
                     TRVIPSVKSAKANYIVVAKDEDKIIGYVYSNISSKYTYSGGFATLKPVDFFDFDSVKT
                     DDVGCLSQFFIKEDYRNTGIGSALFEKSMNWLSTFKAISDLFIFVSNGNDNALNFYVH
                     KGFKVSHQILDGFITVLRNN"
     gene            46556..47563
                     /locus_tag="PKU88_00245"
                     /pseudo
     CDS             46556..47563
                     /locus_tag="PKU88_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416341.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            47830..48519
                     /locus_tag="PKU88_00250"
     CDS             47830..48519
                     /locus_tag="PKU88_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67752.1"
                     /translation="MKKALENSNDSLRYIVKNGIGSVKELYLNEVNRENIDKIDLPSA
                     SAGIYRIGEGIFEYLLIGDICPVIEDIEGNIKEIKDTKLEVLDNIAVNELRRLMASRG
                     LSFNEAREGIQEILLKHRRLKNTDKGYWTLEFEPSAVDYCIYDKLPLESISKALFMTD
                     GFFAIHSSYNYFSVKDLISFTDMKGLEKVYKTLRDIEEEDKECNRYPRFKKSDDASAI
                     YIKYEDVQAVY"
     gene            48606..49835
                     /locus_tag="PKU88_00255"
     CDS             48606..49835
                     /locus_tag="PKU88_00255"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX67753.1"
                     /translation="MSYFDSYRGLPRSVYIIFFAQIINRFGDFVLPFLSLFLVKKLGL
                     SYQDAGIAVMLTSLSTIPGSFAGGKIADHLGRKKSYAFFQLSAAVCLFFCAFSENPQF
                     IIALICMASFSNGAVRPIISAIITDVLPAEKRKMGFSLSYMGINLGVSLGPLVAGFLF
                     NRYIPLIFIGDAVTSLIAVILVLTNIKETLPDYSDNKCLNEEEKSEEGNVFSVLIRRP
                     KILIFLTINIVFNVVYAQHSFSLPMMLDNIFGSSGASNFGVIMSCNALTVIILTMLIA
                     HATSKWKPLSAITIAGLLYAIGFGMITFISSMPLFIISTVIWTTGEIMTVTNFGVYIA
                     NNTPQNFRARFNAISSLSWAVGAALGTSVMGRYMDFFGVKAVWPLAFCLGLAGAAGMY
                     LLESHSNFRVSRAKELN"
     gene            49880..51241
                     /locus_tag="PKU88_00260"
     CDS             49880..51241
                     /locus_tag="PKU88_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX67754.1"
                     /translation="MNGDMTQGSSLKALITFSVPLMLENILQQLYYITDAVILGRFVG
                     KNALAAVGVANPIMSTAIFFMFGVCIGTAIFMSQIFGAAKYEILKREVSTALIAGAVF
                     TGLLSIISVLSARYILIAIGTPEEIMNDAEIYLKILSGGLIFSFLYNFYSFTLKSIGD
                     SKTPFIFLSISCVLNGILCYCFVALLGLGVVGSAAATIIAQVVSSVMCISYVYIKVPM
                     ISLKPNELVMDKSLLRKTIKYSSITALQQISLYMGKLLVQGVVNPFGTNTIAAYNTVT
                     RIDAFVLSPGDNFVVSVSTYTAQNMGARKWKRIIEGYKIGNIIITIYNLAVAAIVFFG
                     AEKLMHLFISASEREVIMIGVEYLRLMPLFYVLTGFCNIFQGIFRGIGKLHITFFATV
                     TQVSARVILALIFAPYFGISGICYAVGIGWVIMIIYEGLIYRKEYLRMKAFDTAQDVL
                     DSA"
     gene            51264..52361
                     /locus_tag="PKU88_00265"
     CDS             51264..52361
                     /locus_tag="PKU88_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HPX67755.1"
                     /translation="MIDYEGQYPVYEISASKIKSNARKIYEMCRANGIEPAGVVKGFS
                     AIPEVAELFIEAGFKTLGDSRMKEIIKLRRAGIKGELMLIRIPMPCEIPELVSFADSS
                     LNSEMETIRLINKEAGLQGKVHGVILMVDLGDLREGFFNREEAIEAAKEVEMLENVKL
                     LGIGTNLGCYGSIKPSEKNLRELCRLAELIEKEIGRSLEIISGGASTSLSLLVNGKMP
                     ERINHLRIGEAIITAKDFPYVWGIEMPGIYRDPMVLKAQVVEVKTKPSYPIGEIFVDA
                     FGNRPVYTDRGMRKRAVLAVGNKDVGDFSKLKPRHEGIEIIGSSSDHLILDIEDYAGD
                     IKVGDVLEFDTFYAAMVFLCSSDCVRKAVVE"
     gene            complement(52449..52628)
                     /locus_tag="PKU88_00270"
     CDS             complement(52449..52628)
                     /locus_tag="PKU88_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-type small acid-soluble spore protein"
                     /protein_id="HPX67756.1"
                     /translation="MDQRRAKAITASPVMTHVTYNGIPIYIENVDEETGIADIHPLDQ
                     PSNKLEVPVTSLVEH"
     gene            53058..53255
                     /locus_tag="PKU88_00275"
     CDS             53058..53255
                     /locus_tag="PKU88_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPX67757.1"
                     /translation="MVGTVKWFNGEKGYGFITGEDGTDVFAHFSQINAEGYKSLQEGQ
                     KVSYDVTNGPKGPQAENITII"
     gene            53444..54034
                     /locus_tag="PKU88_00280"
     CDS             53444..54034
                     /locus_tag="PKU88_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPX67758.1"
                     /translation="MKVKGLIITACIVFALASFLPGCSVSSSNNTNINQEKEEPKVLT
                     IYSGRQEKFIIPLVEKFEEDTGIKVNLISGKATEYAHRIIEEKENTQADVFLSNDGEI
                     LEYLRIENMLSPINSKLLEEVPGNYVGKDNTWTALTIRYRVFIYNTDLITLDEMPDSI
                     FDLTDPKYKGQFTMNRAGNESMITYFASIQLKLKLL"
     gene            54092..54379
                     /locus_tag="PKU88_00285"
     CDS             54092..54379
                     /locus_tag="PKU88_00285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPX67759.1"
                     /translation="MIKVVAKSFAKPDKLDKILKLSAEMVEKTVKEEGCIKYELFQDI
                     KAPEVLVIVEEWESEEALSRHMASEHFRRIVPQLNELREKASEVNILKKLF"
     gene            complement(54428..54844)
                     /locus_tag="PKU88_00290"
     CDS             complement(54428..54844)
                     /locus_tag="PKU88_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPX67760.1"
                     /translation="MEVNTSLTQNIDALFTNIETFTQNEGLLGKPVSQGDKTLIPIIS
                     VTIGYGGGNSSGKMSGGAAGSNQGTGNAGMDALGLGARISTDALALVDGENVSILPVN
                     ANPGNVNQLINQLPQIMGKGQNNQQQNQQQNSQQNS"
     gene            complement(54873..55352)
                     /locus_tag="PKU88_00295"
     CDS             complement(54873..55352)
                     /locus_tag="PKU88_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67761.1"
                     /translation="MTLLFILLLSLLLFIFLLFTVASRIDLSYDSARSDMHLTLRWLH
                     PLLRSVIVKEGNGFLLLVYLLKKRVLAKLMKPKQYTKRSGNILKSLKPTEIHVNTQYG
                     FRDPFVTGLACGAVSAVSQLLNVESLHQKPDFLANSDYINLDATAKLNLGRLLMKLI"
     gene            complement(55598..56056)
                     /locus_tag="PKU88_00300"
     CDS             complement(55598..56056)
                     /locus_tag="PKU88_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPX67762.1"
                     /translation="MSIIIGDNAPDFSLKDNKEQIIRLSDFRGKKVLLSWHPLAWTSV
                     CTDQMRSLEVNWEKFHSLNTVPLGLSVDPVPSKKAWSAVIAIDNVSLPSDFWPHGQVA
                     RDYGIFIEKKGISERANIIIDENGIVRWIKVYPISQLPDINEVLEVLSGI"
     gene            56209..57690
                     /locus_tag="PKU88_00305"
     CDS             56209..57690
                     /locus_tag="PKU88_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX67763.1"
                     /translation="MFKRYFKVCQLFLDSYHKGLLWASRETGFVCCVSVNYMDWKIYS
                     MEEYKNFVITIVLIFSLLIISVIMLAAYINRISKIKRELQIKNEELTASDDKLKKQLE
                     EHERVQHSLITSEKRYLLLFEKMMNAFFIFEPVIDEEGIISDIRFLKVNPGFELHTGL
                     KGAEIIGKKWMEVYNYPNKNLHIYHRILLTGKPEHFETYYEQEDKYYLTNGFKISDNE
                     VGAIFENITRYKKAIKEITMLNEELEHKVAERTDELQNALNELEAFTYTVSHDLKSPL
                     RAIEGYSKFVIEDLESGPGEETAEMIHNIRNISLDMIEMINKLLEYSTMTKTEIAKES
                     VDSGELFKSIINELLSVNTDRNIELKAVTELPVVFADKVMFKQVLYNILSNAVKFTKL
                     KDRAIIEVGCSINEGEYIFHVRDNGVGFDMQYSGKLFGIFQRLHTKDEFDGSGIGLVT
                     VKKIIQKHGGRVWIKGSMGQGATVYFTLPVERGGIYAAGNDCG"
     gene            57665..58795
                     /locus_tag="PKU88_00310"
     CDS             57665..58795
                     /locus_tag="PKU88_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX67764.1"
                     /translation="MLQVMIVDDVDIVRKELKRLKLWGDKTGFVISGEACNGHEALEK
                     LEESKFDMVITDIRMPKVDGIELLKEITGRKLCPCVVLLSDYSEFVYARQGLVLGAFD
                     YIIKPVAEEELEKLLQRAKEYISEKRLEEQRILQLEQKLVEKVDMFFPQSEVSQLIDA
                     IMAGHTKTIENAGSIFEIVCTNMNCDLIKVKSLLNSIMLEITNKISASCKWLDKYTDI
                     NAIRTIDFSGANDLNSIKNRFVSAVGRISGLFARLKHGTEDNGITGQVCRYVLENIDG
                     NITLSAVSESLFMSKSYISEVFKQKTGLSFIEYLTIVKMERAKKLVGDGRLKTYEVAE
                     LMGFKDIEYFSRLFKKYTGITPTEYRQGMEGEKYKDPKKFGV"
     gene            58882..59247
                     /locus_tag="PKU88_00315"
     CDS             58882..59247
                     /locus_tag="PKU88_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX67765.1"
                     /translation="MKQIDVLVVEDSFYSADLSIRVLKKAGFNVRYKIASSSITMREA
                     LKDRHWDLILSDNYMPGFDALQAIEIRDQSGSLAPFVIVSEYIQEIDIRKAMEKGCCA
                     YIAKEDLDKLAQHIEKILE"
     gene            59296..60954
                     /locus_tag="PKU88_00320"
     CDS             59296..60954
                     /locus_tag="PKU88_00320"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HPX67766.1"
                     /translation="MVFNKNEKKLERIEKVTFGNEYREFLGKGELESNQISLRIALTY
                     MIVVALWILLSDKILGLLAADNAVITRISMIKGWVYVLISGILIFVIVSYSLKRVKIA
                     EGKLYDSCQNLSEANMELEHLAYHDQITGVRNKISLIKRINELVSSDDSKKIAILIAD
                     IDNFKYINDAMGHSIGDQVLIKFSSRLKGLAGVDSTVYRLGGDGFVIALEGYEEASSV
                     KRLAESLLKDFKTPLDVNGSKLFITISIGVSMYPENGDGMDELLKNADIALNKAKATG
                     KNRVVFYNRTMNEAVSERVNIEKHLRAALINNEFELYYQPQWDIVQNKVSGLEALIRW
                     KNPELGYVNPLKFINIAEDTHMIIPIGEWVLKSACTTLKELHRQGHEGLSVSVNISMV
                     QLLQDDFVCKVTGILDYAGLSPEYLELEITESVLMESCDALEGKLKILKDMGIKIALD
                     DFGKGYSSLNYLKQLPISTLKIDKSFIDTIESDSKHKSLTNVIVKLGRIMGLSVVAEG
                     VETKEQLDYLTRHRCDKVQGYLFSKPVPGEKVIEAIRGLNVSFR"
     gene            61287..62861
                     /gene="gpmI"
                     /locus_tag="PKU88_00325"
     CDS             61287..62861
                     /gene="gpmI"
                     /locus_tag="PKU88_00325"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002847861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPX67767.1"
                     /translation="MSKRPVVLVVMDGIGINEEEKGNAVKAANTPTLDMLMNHSPYTL
                     IKAHGTAVGLPSDEDMGNSEVGHNALGSGQIFSQGAKLVNESIENGKMYSSDTWRKIA
                     GNCVANGSALHFIGLLSDGNVHSNIAHLKSMIIRAKEDKIKKVRVHALLDGRDVLATS
                     ALDYVEQMEKLFKELNDGSFDGRIASGGGRMKITMDRYEANWDMVRLGWDTHVSGKGR
                     QFGSASEAICIYREELPGVIDQDLPPFVIAENGIPAGTINDGDSVVLFNFRGDRALEL
                     SMAFDYEDFDKFERGRYPKVIFCGMLQYDGDLKLPKNFLVSPPNIKNTLSEHLVKHGI
                     KQYAVSETQKYGHVTYFWNGNRIEKFDDKLEVFEEIPSDRVSFDERPWMKAAEVTDKL
                     IDALKSGKYDFLRANYPNGDMVGHTGSFNATRIAVEAVDLCLARVIKAADEARAILII
                     TADHGNADEMLEKTKGGTVKAKTSHTLNPVPFIIHDKVQRHEIKEGSFGLANITATIT
                     TLLGIDHPDCWEESIV"
     gene            complement(63356..64546)
                     /locus_tag="PKU88_00330"
     CDS             complement(63356..64546)
                     /locus_tag="PKU88_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPX67768.1"
                     /translation="MERITCTISRGLRAPIIRQGDNIADIVVNTVLKASESEGFSIND
                     RDIVAVTESVIARAQGNFASIDDIAEDIKSRFGSSTIGVIFPIMSRNRFAICLKGIAK
                     GAGKAVLMFSYPTDEVGNQLIDIDTFSEKNLNPWSDVLTEGDFRRYFGYSRHTFTGVD
                     YIEYYKSIINEQGIECEIIFSNNPLTILKHTKNVLCCDIHTRARTKRILKENGGEKVY
                     GLDDIMSSPVGGSGFNEVYGLLGSNKSTESSVKLFPRNCQPVVEKIQAMLKEKTGRTV
                     EVMIYGDGAFKDPVCKIWELADPVVSPAYTSGLEGTPNEIKLKYIADNNFAHLKGEEL
                     KKAVSEYIRCKGSNLVGCMEAQGTTPRKLTDLIGSLCDLTSGSGDKGTPIVYIQGYFD
                     NYVK"
     gene            64838..65185
                     /locus_tag="PKU88_00335"
     CDS             64838..65185
                     /locus_tag="PKU88_00335"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPX67769.1"
                     /translation="MIEKRRHKRLPLKLELKISSLFRQDYEIIDDINEGIEIRNISKT
                     GIGFFCTHELPLNYYFDAKIQLTPDSHFYAVVKIIRMEKLEEGYFAGCEFIGLADILS
                     MRVDMYEDELNNI"
     gene            complement(65224..65622)
                     /locus_tag="PKU88_00340"
     CDS             complement(65224..65622)
                     /locus_tag="PKU88_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018671081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HPX67770.1"
                     /translation="MMPMVTFGSKKEKKYVQQKESSYEYKFIEETQTHNHEFLGSTML
                     PSQDVEAHNHRFAGVTSEVIFVPGGHVHTFMTDTDFFFGHIHGVGGTTGLQVPVDGGR
                     HVHFASDTTTFDACHDHDFQFATLIENPLE"
     gene            complement(65809..66000)
                     /locus_tag="PKU88_00345"
     CDS             complement(65809..66000)
                     /locus_tag="PKU88_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscE/YscE/SsaE family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HPX67771.1"
                     /translation="MSGKNAKQQVQEVFSQLNNAKTSLNQALNSAEKPENKQQIQNTL
                     NAIENAINTTSTALSNYKD"
     gene            66175..66927
                     /locus_tag="PKU88_00350"
     CDS             66175..66927
                     /locus_tag="PKU88_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPX67772.1"
                     /translation="MKLISWNVNGLRACIQKGFLEYFREADADVFCIQESKLQEGQVD
                     LKIPGYHQYWNYAEKKGYSGVAVFSKAEPLNFTCGIGREEHDKEGRVITLEYNDMYIV
                     TVYTPNSQEELARLGYRMQWEDEFRSYIKGLDGKKPVVVCGDMNVAHKEIDLKNPGSN
                     RRNAGFTDEERNKFSEFLEAGFIDTYRYFYPDRAGVYSWWSYRFNARANNAGWRIDYF
                     CVSERLAERLISADIHSQVLGSDHCPVELIMK"
     gene            67091..68269
                     /locus_tag="PKU88_00355"
     CDS             67091..68269
                     /locus_tag="PKU88_00355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPX67773.1"
                     /translation="MIKTFDELGMDERLIEGLKKQGITEPTPIQADTVKLALENKDIV
                     GQSPTGSGKTLAFLLPIFQRVDAGKRENQAIVLEPTHELVMQIEKQARLLAANSGIAV
                     TSAAIIGEVNITRQIEKLREKPNIIIGSAGRVLELIKRRKINAQTVRTIVIDEGDRML
                     DEHNLGTVREVIKTTLRDRQLMVFSATIGEKALSTAREFMKDPQVVRIEDRGKISPDI
                     RHIYLAAEKRDKIEVLRKLAASMKPERAIVFINKSEELQLTAAKLKYHHLSACDISGS
                     SSKEERKKALEGFRKGDIKLLVASDIAARGLDIKGVTHIFNLDLPEDPKGYLHRAGRT
                     GRMGEPGTVVSIVTEREVPFLRKCERDLKIKIYEKIIARGKVLDPPQSKTSAGKGAGR
                     "
     gene            68610..70538
                     /locus_tag="PKU88_00360"
     CDS             68610..70538
                     /locus_tag="PKU88_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX67774.1"
                     /translation="MIKIGIIVPNDLVNIAKSAAEGIEDEITILYGSMTEGISLAKQL
                     EECNYDIIITRGGTQVLLTQSNINIPVISIPITPIDVYEAVNEAEKISKDISFIVSQN
                     MISIIESYIKISGRNFKVFQIKEESEIEKKIRELATEGKKVVVGLGTIAKYASSYGLV
                     PIVIKSGKESFLSTIMEAKRIVNATKKEKKDKERIKAIIEHTIEGIICVDKKGHIIII
                     NEVARKLLKCDNNGLVGKMISSVLPDLQLEDTLYNGIMETEVIKNLKDTKVMVYKIPI
                     IVDDEIENAVAVLRDIDEIQKADEKIRQDIAVSGHFAKYTFNDVIGCSESIREIVRIG
                     KEYAKVNSTVLMEGETGTGKEIIAQSIHNYSNRSNKPFVAVNCAALSESLLESELFGY
                     SSGAFTGADRKGKRGLFEQAHTGTIFLDEISEINPLLQGRLLRVLQEKQVMRIGDNKL
                     IPVDVRVISATNKDLHNLVVAKKFRDDLYYRLNVLRIELIPLRYRREDIPCLIDYFLR
                     AYCAKLGKENIVFTSEAAEHLSKYDWPGNTREVRNFCERLAVMAKKTIITLEDVTNHI
                     INIGVNYNNADELVDIVGKNKNLSKDNLNLNNMTKQSIELALIHSNGSITNAAKELGV
                     SRTTIWRKMKKYNLLVKK"
     gene            70735..71895
                     /locus_tag="PKU88_00365"
     CDS             70735..71895
                     /locus_tag="PKU88_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPX67775.1"
                     /translation="MERRTYLQIPGPTNIPDRILRSLSQPLINHRGPEFGELLTKCVE
                     GLKKVYKTKNDILIFPSSGSGVIESAIVNLFSPGDTILTASIGVFSERAATIAEKFGL
                     NVIRITKEWGESVKPEDVKKVLEEDTDKKIKGVFVPHNETTAAVASDIKGISEVIKEI
                     NHPAVLIIDAVSSLACLPIETDDWAIDIVLTGSQKGLMLPPGMGIVSISPKAWELVEK
                     STLPKWYWSYKAVKERMEIKEFPYTPATTLLFGLSEALDIIEEEGLENVIARHELLAT
                     AVREAAKAMGLSIFTEEGYESNSVTAIIMPEGISYKDLAGILKTKFGVVVGGGLQKLK
                     GKILRIGHLGSIHKADIYAIMCAVEAALFELGYKVELGSAARAVSKVFLNDK"
     gene            71962..73041
                     /locus_tag="PKU88_00370"
     CDS             71962..73041
                     /locus_tag="PKU88_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPX67776.1"
                     /translation="MYYTTKEIEHKFVTDVFMHLGVKEEHAKMAADVVVQSDITGVTT
                     HGLARMPMYVRRLLQKTVNPTPNIRQTNDSENLIALDGDNGPGNVVGPTAINMCIEAA
                     KKHGIAAVSIKYSNHYGVGNYYAWRFAEANLIGITMTNSTPSMAPTGGKTPMLGTNPI
                     TIAVPAGKRYPICLDMATSVVAKGKLLKAVEANSKIPFEWAVDKEGRPTDDPNEALEG
                     SLLAIGGYKGYGLAMIIEILSAVISQSAYGLDIGDKFDADCTDQEKIGHFMLAIDVSK
                     FLDLDTFKKSMDQYIETMKNTEKAAGVEEIYVPGELEFLRAEKIKETGIPVPEGIQAK
                     MLKIAKDVGIGSDIKTIDDLFDRYK"
     gene            73114..74154
                     /locus_tag="PKU88_00375"
     CDS             73114..74154
                     /locus_tag="PKU88_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HPX67777.1"
                     /translation="MKQYIDLRSDTVTQPTQEMRDAIYNAIVGDDILGEDPTVKELEE
                     LAAKMFNKEEGLFVSSGTMANQLAVMTFTNRGEEVIVGDTSHIYNLEVGGLSALSQVQ
                     TRPIHVPDGIYDIDILEELIQPKGIQSPKTGLICIENTNNLNAGLVVPVDNINEVCNL
                     AKKKDIPVYMDGARIFNAAIALDMNVKEIVKNTDAVMFALSKGLAAPFGAVLVGSKEF
                     INKARWFKQRIGGGYRQAGFMAAPGVLALRTMISRISIDHDNAKLLGNGLSNIVGLRI
                     DTTRIHTNIVSGSIELLNITIEDFLNRLLSKGIKAKRISETSFRMVTHLGIDESHINY
                     VIESVKDIITDL"
     gene            74443..74772
                     /locus_tag="PKU88_00380"
     CDS             74443..74772
                     /locus_tag="PKU88_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HPX67778.1"
                     /translation="MSEDTILIGGYSNFPSGAAAKEVYGMLGVALEIDPKTGKIVNAA
                     TTLLTDVTKNFVSRILVGQNMNEGLKEPIKSIERRYFGRNKKALIAALMDAHRIYEKY
                     LEEKYNL"
     gene            75054..76145
                     /locus_tag="PKU88_00385"
     CDS             75054..76145
                     /locus_tag="PKU88_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPX67779.1"
                     /translation="MYAERHNKLLEVMRDKGWDCVILWPSPNMYYMTGYYPKPDERLQ
                     IAFIPVQGEPVLIVPRLYSGESKHKCWIKDQRSWIDGDDILALTKDVIKDLGLKDARI
                     ALDDTMGFLQVSTIQAACPKAEFGLAGTVMSPLRLEKGPEEIALMQKSSQISDEVMQI
                     GIDACLSGQNEQQLVDLVEYELRKRGMANGFSNLIASGTHSALPHHVSSKRVPQKGDA
                     VFLDIGGAYERYWSDITRTIHIGPPSAKFIETYEAVKEAQQRASEAVKPGVTAEEVHE
                     TAFGYLEKKGLSKYFFHRVGHGMGLEGHELISINRGNHLVLKPGMTFSVEPGVYFEGE
                     FGIRIEDTVVVTETGHKSLTEFTRDLIVL"
     gene            76209..77789
                     /locus_tag="PKU88_00390"
     CDS             76209..77789
                     /locus_tag="PKU88_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX67780.1"
                     /translation="MKRMRYLALVMVLVMLLSVGCSSSGGEAPGAEAPSEAGSEASTP
                     AKTTLVYGVGVDATTMDPHFANNTPTGNVSFQVHENLTAFDDPKTMKPVGKLAKDWKI
                     SEDGLTWTFNLREGITFHDGAPFNAEAVKKNFDRILNPDLGAPRRSNFKMIDRLEVVD
                     EYTVNIITNVPYAALPQLLTCYNASILSPKASDEYGDQYSAHAAGTGPFKLGEWIPGE
                     SMTLDRNDNYWGEKPSLEKLVFKIIPEDASRVMAVQTGEVDYISNVPPFEIENLKSSG
                     DVTVEMHEGFRTIYLGMVINRPPFDNIKVRQALNYAIDRDAIINDFLEGSATYAIGPE
                     ATSIPGASSDLFKYTYDPEKAKALLAEAGYPDGFEIRFVSPHGRYIMDRQIAEAIQAQ
                     LSKVGIKANLQILDFSLLLNMLPTGKDAELMLMGKGSPASDPDFDLTNYFTTDGTNNW
                     YFISDPKIDELTEKQRATMDLNERYEVLQELQEKVQEELPWASLHYERQIVALGKGVA
                     GVTVWPYEYIDFSKAKFE"
     gene            77873..78793
                     /locus_tag="PKU88_00395"
     CDS             77873..78793
                     /locus_tag="PKU88_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX67781.1"
                     /translation="MFKYLKRRLLMLIPLLLVVSIIVFSMIHLMPGDPAMIIAGEGAS
                     QADIEAVKHKHGLDKPLFVQYFTWLGNIIRGDFGKSLRTGRPISQEIGFRFVNTIKLA
                     TLAIVIGSILGLVMGIVSATKRYSFFDNVTMVIAMVGVSITPFYLGLMSILLFSVKLG
                     WLPMMGVESWKGWILPATTLAFRPAAMIARMTRSNMLEILGQDYILSARALGLPNKVV
                     FVKYALRNALNTVVTVIGLQLGLLMGGAVMTETVFSLPGIGRLAVESIKGRDFPSIQV
                     SVLLIALSFVIINLAVDILYAIINPRISYN"
     gene            78816..79724
                     /locus_tag="PKU88_00400"
     CDS             78816..79724
                     /locus_tag="PKU88_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX67782.1"
                     /translation="MESNNNTASMPMNDAKMEREARIRELKRFWLQFSKRPVAVLGLV
                     IVLIFLVMCITAPLIAPFDPYESDTSKRLLKPGEEGHILGTDELGRDLFSRIIFGSRY
                     SLLIGVGSVGLGALIGIPIGLIAGYYEGKIGNVLMRIMDVFLAFPNILLALIIVSILG
                     PSLYNALIALSIWSIPSFARIARSEVLALKNNEFVEASKAMGSRDSWIVLWHIFPNLV
                     SQLIVWITLNISSAILTGAGLGFLGLGAQPPSPEWGVMLANGRQYIMRAHHLTTYPGL
                     AILFLALGLNLLGDGLRDTLDPRLKD"
     gene            79747..80709
                     /locus_tag="PKU88_00405"
     CDS             79747..80709
                     /locus_tag="PKU88_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX67783.1"
                     /translation="MHTTLKIKDLSVEYHTFDGIVKAVNNLNLEVKKGESIGLVGETG
                     AGKTTTALSILKLLPYAGHVVNGEIEFNGKSLLKASEQEMYRIRGNKISMVFQDPLTS
                     LNPVYNIGTQIRDVLTHHQSLNKEEAIEKAKEMIELVGIPSNRLNEYPHQFSGGMRQR
                     VGIALALACNPELLIADEPTTALDVTIQAQILALMTELKQKFQMSLIMITHNLGIVAE
                     VCDSVAVMYSGEIIEIGSVKKVFNNPMHYYTKGLFGSVPSPDVKISRLNPIKGRMPDP
                     KNLPKGCKFYPRCNAARPECLKMPELIKVEEDHFVACVAGKEGV"
     gene            80709..81659
                     /locus_tag="PKU88_00410"
     CDS             80709..81659
                     /locus_tag="PKU88_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX67784.1"
                     /translation="MSKTFIEIKNLKKYFNVKDGKKLHAVDDVSFNVDKGEIVGLVGE
                     SGCGKSTTGRVILRLLEATAGNVFLEGEDIIQANKNRLKQLRRRMQIVFQDPNSSLNP
                     RKSVRQIISEPLIVQGILKSKEMQEKVYTLMEETGVAFDLVGKYPHELDGGVRQRVGI
                     ARALALGPEFIVCDEPVSALDVSIQAQMLNLFQDLKEKKDLTYLFISHDLSVVRHISD
                     RILVMYVGRLVEEAEADDLFINPMHPYTKALLSAIPVPKIRKESNRILLKGDVPSPID
                     PPPGCRFAKRCWMAKEECFEKEPELKEYSPGHRAACHFVE"
     gene            81687..82445
                     /locus_tag="PKU88_00415"
     CDS             81687..82445
                     /locus_tag="PKU88_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPX67785.1"
                     /translation="MTENKAYILPEMTWVEAEKAYKEAKIAIIPVGSHEQHGPGIKMK
                     VDGVQAYELAKLVAQGVHPLAVVTPPVNIGISHHHMKYPGTVTLKSSTFMAVIEDIVT
                     SLYRFGFKKFLIINAHGGNQYACNLATNDLKEKLGVEIAYTLYLDLVPELVERVPPEK
                     RGHGADPGLAPALYLDEDLVYMDRLEKGKEHWPYKMIGREKVKVNFPYYTHEISDNGV
                     WGDPEGTSKEFGKEICDAVVERLGVFIRDFVKNA"
     gene            82460..83239
                     /locus_tag="PKU88_00420"
     CDS             82460..83239
                     /locus_tag="PKU88_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPX67786.1"
                     /translation="MVNSTKEMKVAIHDLTWPEVDKMRDQVKTVIIPVGSTEQHGPNL
                     CISTDATRAYKFSLRVAKALYPHVLVAPCIHVGISGHHMDFPGTITLRPETMISTITD
                     YVSSLMKHGFKKFFIINAHGGNEGTLQVAVENIRTDFGIRIPFVNYKVLCQDVTAEIV
                     GTKRVDHSGEFEVSDAMFLAPEIVREDAICKGDDGNFPLVYTDIYGKYRINYPLNWKQ
                     VAPSGAMGEAHKATPEKGKQLNDAALKRAVEFLEQFMEDED"
     gene            83321..84046
                     /locus_tag="PKU88_00425"
     CDS             83321..84046
                     /locus_tag="PKU88_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPX67787.1"
                     /translation="MYINQELCVQCHACIPYCPMKAIEYKEESKRVEINIDECVECEI
                     CGNADVCPTGALTMEEMKWPRILRNQFSNPLITHPDTGVPGRGTEEMKTNDVTGRFKR
                     GMVGIAIEMGRPGTGTRFYDVEKVAMAVAKVGVHFEPHNPVTKLMTDNTTGKMKDDVL
                     NEKALSAIVEFDIPIEKTKTVLEQLKTVTPLINTVFSLDLACRLNEDGSNPAVCLAEE
                     AGYKVSVNGKTNVGLGRPLAKEE"
     gene            84049..84672
                     /locus_tag="PKU88_00430"
     CDS             84049..84672
                     /locus_tag="PKU88_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67788.1"
                     /translation="MTHTLHRKGDKEGLSNDYIVFAISAQTVNAKGSAPKFKEFGDIV
                     LKYNPVNYGDMKTGNAYNVGVDKVHESYKDNSIVHAVFTEEDIVAKVLKELREADLGL
                     SIVLSGIVEHTSECCHKISIEPHTIEYSLGIHGKTDSLPEDNVVEISTMCGHGMVSFS
                     LIEHFCEEIEKGRITADEVARKLAKQCHCGVFNPTRAAEILRLMTDM"
     gene            84914..85306
                     /locus_tag="PKU88_00435"
     CDS             84914..85306
                     /locus_tag="PKU88_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HPX67789.1"
                     /translation="MTIYGAAIIAGCMFVGSFIGDALGGLMGIDANVGGVGFSMLFLI
                     LITGSDLFGKKSSAATSEGLEYWQSMYIPVVAAMTASQNVVQAVSGGVMAVLAGLIAV
                     AVGFALLPVLNNMFKSYEVERETEVKGE"
     gene            85303..86073
                     /gene="madM"
                     /locus_tag="PKU88_00440"
     CDS             85303..86073
                     /gene="madM"
                     /locus_tag="PKU88_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HPX67790.1"
                     /translation="MNAVFEVAQNIMINNGLVMSFLFIGLLMWFSTIISKYLTKGRIH
                     SSAIAIILGLVLAYVGGVLTGGNKGIADIAAFTGVGVLGSSMLRDFTIVSTAYGASIS
                     EIKKCGIVGIISLLLGVVSAFLIGAIFAVCFGYADAVSITTIAAGTVTFVVGPVTGSA
                     LGASSPVITISIAAGVVKSIAVMVVTPFAAKRIGLTTPKAAMIYGGLMGTTSGVAAGL
                     AATDTKLVPYGAMTATFYTGLGCLLCPTVLFWLTRLMF"
     gene            complement(86172..87122)
                     /locus_tag="PKU88_00445"
     CDS             complement(86172..87122)
                     /locus_tag="PKU88_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPX67791.1"
                     /translation="MKNYYYDYVLALAEERNFTKAAKKLCISQPSLSQCITKVEKELD
                     VSLFNRSTNPIEITEAGRIFIDTAYKIRTLQYDLENKTSDIADFKNSSLTVGMTPYRC
                     STILPGILKAFTTSMPKVKFHLITGTMQELSNYAQEVSIDIFVSTESYLNKNIFNYKV
                     IAREIILLTVSKSNPINNKLKSYQLSYDDIVNANFDSKKMPIELLSYFKDEPFLLVNT
                     SQHLYKKSVELCRLSKFEPNPFLITANIHTIFNLTLSGLGVSFTPHSFVKYGNLANHP
                     IYYYLDNVNSSRNLIAAYKKNHYLSKPARKFINIMKAVLN"
     gene            87548..88546
                     /locus_tag="PKU88_00450"
     CDS             87548..88546
                     /locus_tag="PKU88_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HPX67792.1"
                     /translation="MKKLVSLLMVVILIVALSGCSGTTGGPAEEAKEPAEKSSKYPSK
                     AIEIVVPYAAGGGVDMNTRAIQPFLEKELDTTVVVMNKPGGGGVLGVTYAIMSEPDGN
                     RLLLTNLIAMCSASALNTLQVDLLEGVEYIGGTVVDPGVIAVSKNSKFNSLDEIIEEL
                     KKNPGSLSFGVTGETSLDNLICKNLEVITGAKFNTVSFDGGSEAMAALLGGHIDIMGG
                     TLSEMLTYHKSGEVKSLAVGHGERIDSISDVPTFDELGYKMAVTAAKRAIMMPAGADE
                     EIVKVLQDAVKAAASSEEFAKECESLGLMASYFTPEECVKTVTEVSEFLNGLYGKK"
     gene            88786..90255
                     /locus_tag="PKU88_00455"
     CDS             88786..90255
                     /locus_tag="PKU88_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPX67793.1"
                     /translation="MRIQNMEMLQNHGNKVGRVHVAQMLEAGMNMVDSYIGASRLISR
                     KDNWLIFDGKEFEMDNDPRSGTAKYDLDKFDRVIVIGAAKGVQRCAILLEEVLGEYLT
                     GGHVIGKHGDDIICKKIGVTLAGHPIPDQMCVEGCKKIHEWIKDINEKDLIITIAGSG
                     VSSLMTWPIAGFTIDDLRNLTHMMQIEKGALTKDLNAIRNHLDRFKGGKILRYMKKAT
                     IVNLATNDLGGASWSSPGIRETYEELMKGNRFLPTIADSTTFQQAVEAFHKYDAWNRI
                     PKHIQKFFTTADPNEETVKMDEYEKMNSRLFKLTSRINTIYPAVIKKAEQFGYNAYIL
                     SDTITAEAVEAGKVISSIALNIQNMNQPLKKPCVLISSGELIVTVGKENGVGGRNQEF
                     CTAAALRIAGSSKIVIGSADTDGTDGPGGFIVDGVRTCLAGAIVDGYTCQEAAEKNIN
                     LEASLKRHDTSSDLWDLGCGIKSTQSISALDLRIVAIME"
     gene            90286..91524
                     /locus_tag="PKU88_00460"
     CDS             90286..91524
                     /locus_tag="PKU88_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HPX67794.1"
                     /translation="MSRIEDIRAFEMLNARGKPTISVELTTSNGIAVEATTPTGTSKG
                     KYEAYELYDGEKRYQGRGVKKAVENVNGIIRKTLIGMDVSNQSAIDQCMIELDGTKNK
                     SRLGGNAILPVSVAVCKAGAAEAGLPLYRYIGGLSAKRIPNMISTVITGGEYSLSGLE
                     FEDYILVSDRKEKSVEDGVEMISSLRRRLEEVLRAKYGAFPDTTGALAPPLSSSKEAF
                     EVMLGVIQELGYDNYFTLGLDVAANELFDESNGSYRLMDRIVSPEELLEYYCSLVSEY
                     PITYIEDGFEQNDFKHFAQLKKAVPQIQIVGDDLFATNIERLKEGINNDSANTLLLKM
                     NQIATVSETIKTCNYAHQNGYDVTASLRSGETIDSFVADLAVGLGTHQIKLGSPVRGE
                     RNAKFNRLMRINYELLSVGL"
     gene            91608..92054
                     /locus_tag="PKU88_00465"
     CDS             91608..92054
                     /locus_tag="PKU88_00465"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HPX67795.1"
                     /translation="MSLVQIFSIIMVIAGALGIYGALQLDIVYNNIVGPAVMPLIYSI
                     ALLALVAVYFAKGRNAPKIEWNMMLKPPKSDAWIFYILNIVMFILLYLLGTIPSILVF
                     SVGTSLCLKRMNLVKTIVFSALWVAALYFIFVVYLGVQFSTGILIK"
     gene            92074..>93356
                     /locus_tag="PKU88_00470"
     CDS             92074..>93356
                     /locus_tag="PKU88_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HPX67796.1"
                     /translation="MAETLSYLMLGFQEVLVPTTLLMTFIGVVMGTVVGVLPGLGPSA
                     ALSVLLPMVYGKEAIPSLAMLAGIYYGAMFGGMITSISINVPGESASVVTSFDGYPLA
                     KQGKAGMAMGISAISSFCGGTFGIILLTIMGAALAKFALAFGPPEYFAIYLFTFVAIM
                     TIGSGGKIIESAIALIFGLLVSCIGLDVMTGTARLTFGSVNLLAGVDFLPAVVGLFGL
                     SEIVLSLSEGEKIEIDKKKGNYKQYSFKNVFPKLHDFIHSLPAIIRGGVVGFIVGVLP
                     GAGATIATFLTYNIEKRVSKDPDSFGKGNIRGVAVAEAANNGAASGAFVPLLTLGIPG
                     SATTAILIGAFIMVGITPGPALFSEHPDVAWGLISSMYVGNVMLIIITTAFIPFFIWV
                     LGISQKTLPIIVASLCVVGTYSLNNSVFDVLLMLV"
     assembly_gap    93357..93456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <93457..94576
                     /locus_tag="PKU88_00475"
     CDS             <93457..94576
                     /locus_tag="PKU88_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPX67797.1"
                     /translation="SIAITCGSRGIDNYPLIIKEIVQFCKDKGANPFIIPAMGSHGGA
                     VAEGQRLICETNGVTEEYCGCPIKATMSVTRIGVTEDGSFDVCIDSYAAEADGIILVN
                     RIKNHTALRGDYESGLMKMMAIGLGKQYGASLCHRAGYERMAELVPMIGKTVLNNASV
                     LFGVGIVDNAYDKNYKIAVLTKDEIPIVEKELLKEAKRLMPRLLPGSADVLVVDRIGK
                     NISGSGADPGITGRLTYKYSGVESFQAGKVAVLDITDETHGNIHGVGYVDVINRRVFE
                     KADLDKTYPNSITSTTLMSDKIPVMMKNDRDTIKCAIKTCQPADPENIRLIRITDTLH
                     ISEILISECMVDSVRHIPNLEITDEIIDWEFNEEGNLW"
     gene            94633..95358
                     /locus_tag="PKU88_00480"
     CDS             94633..95358
                     /locus_tag="PKU88_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX67798.1"
                     /translation="MYINKGLCIKCCACIPYCPMQAIEYNSEKKQVEINANECVECEI
                     CKNANVCPKDALIMEELKWPRILRSQFSNPLVTHPDTGVPGRGTEEMKTNDVTGRFKK
                     GMVGMAIEMGRPGTGTRFSDVEKVAMAVAEAGVHFEPYNPVTILMTDKETGKLKDDIL
                     NEKVLSAIIEFDMPINKTKPVLEKLKSVIPLIDTVFSLDLAYRLTEGDSNLAVKIANE
                     AGFTVSINGKTNVGLGRPLAKEE"
     gene            95362..95982
                     /locus_tag="PKU88_00485"
     CDS             95362..95982
                     /locus_tag="PKU88_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67799.1"
                     /translation="MTHTLHRNGNFESLSNDYIIFAITAQTVNAKGSARKFKEFEDIV
                     LKYNPINYGDMKTGNMFNIDISKIQEGYRDNSIVHAVFCDEDTVAKVLNELKEADLGI
                     SIVVSGILDRVSECCHEKGIKPHTIEHSLGIHGRVDYLPNDNVLEISTMCGHGMVSFS
                     LIEYLSEQILKDRITVEEAAKKLAKQCHCGVFNPARAESILRAMTK"
     gene            96454..97650
                     /locus_tag="PKU88_00490"
     CDS             96454..97650
                     /locus_tag="PKU88_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX67800.1"
                     /translation="MNYDIQLKNNINKNYIFTLLQNIDLTRGIWMIYLAGKGMSLTQL
                     GLLEMIFHIISFLMEVPTGAVADIFGRKISRILGRVLSLISVVILLAADGFLWFAISF
                     VFTALSFNLESGAGEALIYDSLKEIGEEDRYMKISGRKEVFYQAAGVISFLAGGYMAT
                     KSYNIAFAITIIIGAAAVLQSFSFKEPTIGRNKEEQKGRNIFLNQLKESLRVVKSNPR
                     IVSLIVFTEIILTFCTCIFFYLQNFLKGEGYNEAIIGVVYAASFVAAALTASWVHRIE
                     RVIKEQGILLIIPFVTVACLWGIALSPYHFVFFILLTVTEEIVYIAMSDYINKMIPSE
                     NRATILSFSSMVFSFFMIAIFPLVGLLGDRYSLSYAIKCLGGLGIVFVVINSVLLLNP
                     GRKIRK"
     gene            97742..98683
                     /locus_tag="PKU88_00495"
     CDS             97742..98683
                     /locus_tag="PKU88_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPX67801.1"
                     /translation="MKLDRLVSIIVLLLRKERVQARELAEIFEVSVRTILRDIEAINL
                     AGIPIVTYQGANGGIGIAEGYRLDKSVLTENDMSTIISTLRGIDGTIPDSRHGILMEK
                     LRNALPSSQLEALDAKVQQLIIDLSPWGPNELLKYSVTCIRKAIENRNEIEFEYIDST
                     GKRTNRRVEPYTLVLKGQKWYLYAWCHTRQDFRLFKLSRMRGLEVTTAVYQPKKVSLE
                     RLDWEDPWSTSESMVFLRLVFEKEMESIVTDWFGDNVEKLEDGRLLANAVLPENNWLY
                     GFILSFGAGAEVIEPAHIRKILAEISKQIYEKYSMET"
     gene            98742..99200
                     /locus_tag="PKU88_00500"
     CDS             98742..99200
                     /locus_tag="PKU88_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPX67802.1"
                     /translation="MDYNIELKEQGLQPVLSVRKTTSVGNLPREIGKAYGSIMQYLQE
                     LGEQPAEAPYTAYYNLDMEHLDVEMGFPVSKQLAGKGEIKAGSIPAGKYAECVYKGPY
                     AEMAPVYEAMNKWIAEKGLEAVGTSYEVYYNSPAEMPESELLTKIMLPVR"
     gene            99223..99846
                     /locus_tag="PKU88_00505"
     CDS             99223..99846
                     /locus_tag="PKU88_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPX67803.1"
                     /translation="MKYDWKKQDKELYLPKNKPEIVMVPPMWFFMLDGRGNPNNEDFA
                     KAVGVLYSLAYAVKMMLKKGVTPEGYYEYSVFPLEGIWDLAPEARELEALDKDSLIYT
                     IMIRQPDFVTEEIAQRALEAAAKKTKHPLLSKVRFGCIEEGLCVQMLHIGHYDSEPAS
                     FAVMEEFCRANNLNRISKVHREIYLSDARKTPAEKLQTVLRFKVEKI"
     gene            99883..100326
                     /locus_tag="PKU88_00510"
     CDS             99883..100326
                     /locus_tag="PKU88_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HPX67804.1"
                     /translation="MSEYDLILDGSHTPEYDEIVEYINMPARDLWRQINGFIQQRYKS
                     LPRIMYSVCSGKPGWNVKYQKSGKSICTLYPEQDGFVALVVVTLDLMPVIEALSGELT
                     EETRQTIRSAKPFNGTKWLMLQVKNDLQLEDVKQLISLKQGAIAK"
     gene            complement(100384..100875)
                     /locus_tag="PKU88_00515"
     CDS             complement(100384..100875)
                     /locus_tag="PKU88_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67805.1"
                     /translation="MNPFIKKLADSKFMTKAQGNYIEEAVMRKDSIIISGHKGFGILP
                     LFATISATAKSNFKIKQIKGFEDLGDEADYYLIADLSNIDYAKLIAEAMLKQGSSFIA
                     LKDPDHPYSVFKILGDVYKINGDTSKVYQLIECVKIDGVPQLSKITRITLNENGKPVK
                     TDF"
     gene            complement(100881..102356)
                     /gene="gcvPB"
                     /locus_tag="PKU88_00520"
     CDS             complement(100881..102356)
                     /gene="gcvPB"
                     /locus_tag="PKU88_00520"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPX67806.1"
                     /translation="MMKNYNSLIFELSKEGRKAYSLPPCDVSDISLDSVIPPSLMRDS
                     EPELPEVSEVDVVRHFTNLSNKNYGVDTGFYPLGSCTMKYNPKINEDMANLSGFSKIH
                     PLQPEATVQGALKLMYELDGMLSEISGMDRVSLQPAAGAHGELTGLMIIKAYHESRGD
                     KKRKKIIVPDSAHGTNPASAAVAGFEIVEIESDKNGAVDLDRLKAVLSDEIAGLMLTN
                     PSTLGLFEKNIKQIANLVHEAGGLLYYDGANANAIMGIARPGDMGFDVIHLNLHKTFS
                     TPHGGGGPGSGPVGVKSELAKFLPVPVIEKMGDTYILDYDRPDSIGKVKSFYGNFGVA
                     VKAYAYILSMGGEGLTDVSRKAVLNANYMMSKLKKYYELPIDQVCKHEFVLSGLLEND
                     NHIATLDVAKRLLDYGYHPPTIYFPLIVNNAIMIEPTETESLETLDSFIDVMIKIAEE
                     AKEAPDILKTAPHGTVVRRIDEAKAARSLILKWDGTCMNKE"
     gene            complement(102353..103693)
                     /gene="gcvPA"
                     /locus_tag="PKU88_00525"
     CDS             complement(102353..103693)
                     /gene="gcvPA"
                     /locus_tag="PKU88_00525"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPX67807.1"
                     /translation="MHRYLPHTEDDERLMLESIGAKSTDDLFTDIPRELRLGRKLNLN
                     NPLSEIELSDHMKGLSNKNKSSDDLICFLGAGAYDHFIPSVVKHLAMRSEFYTAYTPY
                     QPEISQGTLQAIFEYQTMICSLTGMDVTNASMYDGATACVEAAMMAVENARRSSIIVS
                     RTVHPEVRKVLKSYMRFRDVEVIEADMADGITDVEKLKPLVEAGIAGVIVQNPNFFGI
                     IEDLTEIEKMVHANKALLIDYADPISLGILKSPGESGVDIVAGEGQSLGNSLSFGGPY
                     LGFLATTSKLMRKMPGRIVGQSEDADDKRAFVLTLQAREQHIRRYKATSNICSNQGLN
                     ALAAAIYMTTMGKKGLKEAAEQSAKKAHYAMKQLTSSGKFKPLFNKPFFKEFAVTGET
                     PAGKINDELLKHNILGGFELDKEYPELKNGLLFCVTEKRTKAQIDKLAQVMEVV"
     gene            complement(103695..104078)
                     /gene="gcvH"
                     /locus_tag="PKU88_00530"
     CDS             complement(103695..104078)
                     /gene="gcvH"
                     /locus_tag="PKU88_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPX67808.1"
                     /translation="METKSGLLYSKDHEWVRVEGEKAYVGITDYAQHALGEIVYVELP
                     AVGDELNAGDVFSVIESVKAASDSYLPVAGKVLEVNEALSDSPQLVNEDPYGSWIVVV
                     EITDKAGLDELMSEQEYQEFCDKEA"
     gene            complement(104111..105217)
                     /gene="gcvT"
                     /locus_tag="PKU88_00535"
     CDS             complement(104111..105217)
                     /gene="gcvT"
                     /locus_tag="PKU88_00535"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPX67809.1"
                     /translation="MSEPKKTSLYDVHEKYGGRVIDFAGWALPVQYEGITAEHEAVRN
                     CAGIFDVSHMGEVEVRGPQATDFVQNLVTNDVSVMNENQVIYAMMCYPNGGIVDDLLV
                     YKHANDFYLLVINASNVDKDFQWMKDNIGNYDAELKNISEDVSEIALQGPKAEEVLQE
                     LTDTNLSEIAFFYFKTNVKIAGKNCLVSRTGYTGEDGFEIYMKHEDSEHVWDKILETG
                     KDKGVKPAGLGCRDTLRFEANLPLYGNELSPDITPLEAGFGMFVKLGKPEFIGKEALV
                     KQKEEGLKRKIVGFEMEGPGIPRHGYDVLSDGKVIGFVTTGYSSPTLKKNIGLAMVDI
                     EYSELGTPLEIQIRKNAVKARVVDRRFYTKKYRK"
     regulatory      complement(105308..105382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(105386..105486)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(105578..105868)
                     /locus_tag="PKU88_00540"
     CDS             complement(105578..105868)
                     /locus_tag="PKU88_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67810.1"
                     /translation="MELKEPLVCTKCSGRHFTAKREATYLYSYDLENKKSEALPFLFD
                     NRELIKSHEYLQCEQCGAKFPCTLDERSNTINFTIEQKAVRSDHVTNPEFLG"
     gene            106143..106355
                     /locus_tag="PKU88_00545"
     CDS             106143..106355
                     /locus_tag="PKU88_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67811.1"
                     /translation="MKEFLSNNGIIYEYIDITDSMKNLKIYLKLRDTRPEFEEIKRIG
                     RVGIPFIMLDNGEKLIFDQPELDELK"
     gene            106391..106798
                     /locus_tag="PKU88_00550"
     CDS             106391..106798
                     /locus_tag="PKU88_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67812.1"
                     /translation="MKKRFVIYGLAGWIAETIFTGLGSLFKGDLTMHTWTYIWMFPIY
                     GLMILLEPVHDNIRNAPVVARGGVYTIIIFGIEYTTGWILREIIGKCPWDYSAWPYSI
                     DGIITLSFIPVWFFGGLIFEKLHDYLIRRKILM"
     gene            complement(106803..108410)
                     /locus_tag="PKU88_00555"
     CDS             complement(106803..108410)
                     /locus_tag="PKU88_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HPX67813.1"
                     /translation="MQFDAHKYNYPSRRNIIYARKGMIAASHPLAAQAGLEMLKKGGN
                     AIDAAVATAACLTVVEPTSNGIGGDAFALVWSEGKLHGLNASGPAPASIPAEIIEKIQ
                     SRGIPKHGWMPVTVPGIPSAWSALSKRFGRLPLSEALSPAVAYAYEGHPVAPTVAKSW
                     ESAYRTYRIALKDELFRHWFDTFAPSGRAPKAGELWSSSAHGKTLQEIGETNAESFYR
                     GALAEKISEFAGKTGGYLSLKDLESFEPEWVEPISINYRGYDIWEIPPNGHGIVALMT
                     LNILKGFDFELRDSIDTYHHQLEAMKLAFADGIRYIADRRSMKVSTEELLSDNYAAER
                     RKCIGSKALIPTPGRPPEGGTVYLAAADGEGNMVSYIQSNYMGFGSGIVIPGTGISLH
                     NRGCNFSTDPSRENCIAPGKKPYHTIIPGFLTREGRAIGPFGVMGAFMQPQGHVQVIM
                     NTLDFHMNPQDCLDSPRWQWIAEKDIEVEEGFPLDAVQGLINKGHNIKINPYSGSFGR
                     GQIIWRDERNILVGGTEPRTDGAIAAW"
     gene            108677..109696
                     /gene="galE"
                     /locus_tag="PKU88_00560"
     CDS             108677..109696
                     /gene="galE"
                     /locus_tag="PKU88_00560"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPX67814.1"
                     /translation="MAVLVTGGAGYIGSHCVCSLIDRKEKVVVADNLQTGHEGAVHGE
                     ARFYKGDIRDGKFLDKIFNENDIDTAIHFAADSLVGVSMKEPLSYFDNNVYGTEVLLS
                     AMNRHNVNRIVFSSSAAAYGEPESIPILETDRTEPTNAYGETKLVMEKMMKWADIAHG
                     IKYIALRYFNVAGAHESGRIGEDHCPETHLIPLILQVPLGKRESISIFGDDYDTYDGT
                     CIRDYIHVTDLTNAHIMALDRLRGGAASTVYNLGNGEGFSVKEIVEAARRVTNHPIPA
                     VICPRRAGDPSTLIASAKKAGDEMGWQPRYNSIDEIIATAWKWHKNNPEGFKGKRIVE
                     GDRQK"
     gene            109693..110376
                     /locus_tag="PKU88_00565"
     CDS             109693..110376
                     /locus_tag="PKU88_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67815.1"
                     /translation="MKEIIKRMAKLFFGLFLYGLGIVMTINANLGLAPWEIFHQGLSI
                     KTGITMGQASIYVGMVLVGLDSLLGERLGWGTLFNMLFIGLFMDILMLNHLIPVFQSV
                     ILRFAMMLLGMFVIGIASYFYISVGLGSGPRDGLMVALTKKTGKSVRFIRNSIEITAL
                     AAGYILGGSVGIGTAIMAITAGYFVQFSFKLFKYNLKETKHRFIDEDLKFLYDRVRGK
                     KEDRKAEDQ"
     gene            110403..111359
                     /gene="menA"
                     /locus_tag="PKU88_00570"
     CDS             110403..111359
                     /gene="menA"
                     /locus_tag="PKU88_00570"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HPX67816.1"
                     /translation="MSLKSFLKLVEIQTKVASVIPFALGTLYTVYHFGIFNPRNFIIM
                     FISLLSFDMATTAINNYYDYKRANKTQGFNYESHNAIVKCNLKESTVKLTITALLTVA
                     SVFGLMLFLNTSPVILLLGVLSFAVGILYSFGPIPISRTPFGEVFSGLFMGFVITFIS
                     IYVHVYDTGIAGLTYSGSMVGISFNLTEGIYIFLLSLPAVNGIANIMLANNICDIEDD
                     IENRRYTLPIYIGKERALWVFKALYYISYADLLLLLVLGVTPAATVITLFTIFPVSSN
                     IKSFFEKQTKKDTFILSVKNFLIINVVQVLAIGTTIIIEGLI"
     gene            111846..112730
                     /locus_tag="PKU88_00575"
     CDS             111846..112730
                     /locus_tag="PKU88_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX67817.1"
                     /translation="MKDQAVVSGSRRGYLNKDFEFFHLKDRKIMEFELHFHDFNKIII
                     FMSGNVTYLIEGRAYKLKPWDMLLIGSSEIHKAIVGTSGTYERMIIWVNSNFLHKYKS
                     PEGDLLRCFDIASKEKRNILRLNPKLLRQGKHILSQLKEECKSGGFGSQVLKNSLFLQ
                     FIVYLNRIYLEPDSIEHKADGDYDESIGEVIRHINENICEDMPIEGLASKFYMSKYHL
                     MHKFKSQTGYTIHSYILQKRLMMANTLIKSGKSVAEACTECGFGDYSSFVRAFKRMYG
                     LSPKKHIKMVEELEKAGK"
     gene            112854..113675
                     /gene="speD"
                     /locus_tag="PKU88_00580"
     CDS             112854..113675
                     /gene="speD"
                     /locus_tag="PKU88_00580"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPX67818.1"
                     /translation="MLMKAENGKLKLYGFNNLTKSLSFNIYDICYTKSREDRKKYIEY
                     IDEQYNSTRLTKILSDVTEMIGASVLNVAKQDYDPQGASVTLLISEEEVPLYVLDPSC
                     NRGIITPIRENIVGHLDKSHVTVHTYPESHPQNEISTFRVDIDVTTCGTISPLKALNY
                     LIGSFDSDIITIDYRVRGYTRNIRGRKHFIDHKINSIQNFIAKDTINRYDLIDMNIYQ
                     SNIFHTKMRIRDLDLNNYLFEIEEDDLTPKEKKDISKKLDREITEIFYGINLPKI"
     gene            113778..114395
                     /locus_tag="PKU88_00585"
     CDS             113778..114395
                     /locus_tag="PKU88_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPX67819.1"
                     /translation="MMLSFKKFIYNTIKKLYISSELLKSEEKKLLHISDTPLCFYSEL
                     KRLIGWLKPAYIVHTGDLTDNIKLELYPALIHEHEKGVKVLADMLEASGAEVILAIGN
                     HDNIDVVNKHFVRSRIIIEAETINLEGLSFRISHFPKGVQENPSRYNLFGHDLTLKNG
                     CIDDKLYFNGITYISIIGLESGEFTTLNYPLYTNDARVGRRKVGL"
     gene            114423..115430
                     /locus_tag="PKU88_00590"
     CDS             114423..115430
                     /locus_tag="PKU88_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67820.1"
                     /translation="MLSLVRGGPKVLLLESSRIEELRNYFIQEKGAEAYNPSDVFENA
                     GEDHTILFIIDKIKEAVDFDEADNGILINEEPDVILCNMLNDKKIDLVSRSRIATRII
                     VLRAMGDLERVVRDISGDYQCIVGSPLDIIKSSDEKGTLVVVTEKPLHRNISYKKDIY
                     EKGLLINEKYKPLIRSLRSRALKYLNNGIGNKDWYELEIRIYDKYGEYDLHYTRLINI
                     MESLEIGIILGASWGKDYPRPMMAIEIYRVRFFTYYDPIYIKKLLMGLEFLEDGTRIV
                     DYDLFYKRKKLYWAETVEKDIKERHLIAAKYRKEILSRLNEEEINKMYEIENNILATR
                     K"
     gene            115508..115909
                     /locus_tag="PKU88_00595"
     CDS             115508..115909
                     /locus_tag="PKU88_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67821.1"
                     /translation="MYDDEKDDIELDYELCESCPFHRQRPPTFGPFGPQFQPPGVGPG
                     SSDGPPSGPPPSITPQFGGPQGIGTLAVDPGSIRRCRFSFVYLQLRNGREFWMWLTFV
                     GRRSISGWRWTGRRWVFFGVDLRQIESFYCY"
     gene            116103..117512
                     /locus_tag="PKU88_00600"
     CDS             116103..117512
                     /locus_tag="PKU88_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67822.1"
                     /translation="MGITLTTLHWVYLIFILLVIAFIVMRKDTSFICVAGILVLGFIA
                     TSTITHSVTGVFNSFAYAIKELLGTILIISIIYGMSKVLMKTGINEAMVYPFTRLIRT
                     PALAYWVIGILMMIISWFFWPSPAVALMGAVLLPVALKAKLPAMGVAVAMNLFGHGIA
                     LSGDFIIQGAPKLTADGAGIPVIDVVAASVPLVIIMGAVTTISAFWILRKDLKSGALK
                     VEDENMRKSMIVQINDGKASMKEIPPVVKKLLAAMVPVLFAVDILMMFILKLQGGDAT
                     ALIGGTSVLILIIVSFFAYSADPLGKITEHLIEGLQFGFRVFGPVIPIAAFFYLGDSA
                     IIDIFGKVLPEGSNGLVNDLGVALAGAVPLNRGIGAVTATAVGAITGLDGSGFSGISL
                     VGSIAKLFSIALGTGAATLTALGQVAAIWVGGGTLIPWAVIPAAAICGVDPFELARRN
                     LKPVVIGLIITTIAAVFLV"
     gene            117611..118534
                     /locus_tag="PKU88_00605"
     CDS             117611..118534
                     /locus_tag="PKU88_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPX67823.1"
                     /translation="MKKLKAVIIMLICTAIAAAGCSTADNKEPDITEIPKLKIGVMPA
                     ADSAPIFLAEEKGYFKELGLDAEIQVYTNAANRQSALQSGELDGAMVDLIAFVNNVHN
                     GFDIKITTSTDGSFPFLVNKDLKEKKNIKVGMMEVSVTNFLSEEYLGDKYEMEKVYIN
                     EIPARLEMIKAGNLDIANIPEPMASMGELNGLKKVVYENKDDFMPEAMAFTGKALKEK
                     EKAIELFHRAFDKAVDDIKRDDTLAREVLIEKLQLKPGIKDKIILPVYHKTRLPDEAY
                     IRKIVEWVEKIQNIKVNVKYDDMIEGKFINQ"
     gene            118531..119259
                     /locus_tag="PKU88_00610"
     CDS             118531..119259
                     /locus_tag="PKU88_00610"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX67824.1"
                     /translation="MIEVNNLQVSYGNVRALEGISLKVERNATCAIIGPSGCGKTTLL
                     YTLAGLIRPEEGTIYIGGEELSGIRRSTGIILQDYGLFPWKNVWNNVALGLQVRGETK
                     EKISETVSNILAVLGIGHLSCKYPAELSGGEKQRVAIGRTLAMKPDLLLMDEPSSALD
                     AITKEHIQNLILKLYKSAPLTMVIVTHSIEEAVFLGQKIIVMGKAKIKHVLDNPYFGD
                     EELRSRMEYCKICLEVRKWMDRGE"
     gene            119261..119995
                     /locus_tag="PKU88_00615"
     CDS             119261..119995
                     /locus_tag="PKU88_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPX67825.1"
                     /translation="MIRSKSLYGTLMIFLFWYILYIMLQSPVIPSPFAAVLNFITILP
                     AVLLPHELASLARIIAAAAFSLLLGLPLGLLTGMNNKADRLITPIVYILYPIPKVAFL
                     PVLMILFGLGNASKIILISIIVIFQIIVTTRDGVHELGKEYFLSAESLDMSRAQLYRH
                     IIIPAIMPKIITALRLTVGTSIAVLFFSENFAARYGIGYFIMNSWVVVNYVEMFSGIL
                     ALSIMGLLLFKIIDKLESRFCAWMEV"
     gene            120252..121427
                     /locus_tag="PKU88_00620"
     CDS             120252..121427
                     /locus_tag="PKU88_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX67826.1"
                     /translation="MKKLLSLTLVVAMALSLLTGCAQPAPAPSGEPAAPAEEEVIKIG
                     VFEPLTGANAAGGELEMEGIELAHELYPEVLGKKVELIVVDNKSDKVEAATAAARLVE
                     KEKVTAIIGSWGSSLAMAAGDIVKNAKVPAMGTSCTNPLVTQGNDYYFRVCFIDPFQG
                     VVMSNYAYNGLGAKKAAIVQEVSNDYAVGLAKFFTDNFKKLTGDENSIVAVANYNTGD
                     QDFTAQLTNIKAQNPDVIFAPGNFTESALLIKQARQLGIKTPFIGGDTYETPEFAEIG
                     GDAVEGVVFSTFFATERPITSESTKFLDAYRAKYPGKEPAAVTALGYDAYLILIKAIE
                     QAGAADSASIHNALLNIKDFQGAAGVVNFDENGDAVKSAVIKTVKDGKFTFMDVVEP"
     gene            121820..122713
                     /locus_tag="PKU88_00625"
     CDS             121820..122713
                     /locus_tag="PKU88_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPX67827.1"
                     /translation="MTLELFLQHLANGISLGSLYALVAIGYTMVYGILRLINFAHGDI
                     FMMAVYFAFFGVMTFGMPWYVSFIIAIALTGVLGITVETLAYRPIREAPRISILISSI
                     GASYLLENLAIVIYGGRPKTFPQIPLFTDMVQFGTVHIQMLTFMIPVVTAVLLMLLMY
                     LINKTKSGMAMRAVSKDFETAKIMGIDVNRTISFTFGIGSLLAAVGGIMWGLKFPSIL
                     PLMGVMPGIKCFIAAVIGGIGNIQGAVIGGFILGIGEILLVAFLPELSGYRDAFSFIL
                     LILILLFRPTGIMGEKISEKV"
     gene            122716..123756
                     /locus_tag="PKU88_00630"
     CDS             122716..123756
                     /locus_tag="PKU88_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPX67828.1"
                     /translation="MKRNTIYTITAIIVLFVFVYFGNKYLDSYLLRILNLCAIYTVLG
                     LSMNLINGFTGLFSLGHAGFMAVGAYTTAMLTLPIATKQMNFFLAPLVSPLDKIVWPF
                     LPSLLLAGILSAFVAFLIGAPVLRLKGDYLAIATLGFSEIIRVVFTNTQTITNGALGL
                     KGIPATTNLWWSFGTAVITVFIMVSLINSSFGRAFKAIREDEIAAESMGISLFKHKVL
                     SFAIGAFFAGIGGGLLGNMMGTIDPLMFRFFMTYNILLIIVLGGMGSITGTIISAFII
                     TIAQEVLRFLDEPIFPNVPALPGLRMVVFSILLMLVVLFFRQGIMGTREFSWDKFFRF
                     FTKRPLSKGGAK"
     gene            123756..124562
                     /locus_tag="PKU88_00635"
     CDS             123756..124562
                     /locus_tag="PKU88_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX67829.1"
                     /translation="MNVLRTDNITMQFGGLTAVKEFNLELNKGEIVALIGPNGAGKTT
                     AFNMITGVYTPTKGKVFLNQKSTVDGKEEHKERDITGLRPDQITKLGIARTFQNIRLF
                     KDLSVLDNVLIANHLRIDSNVFEAIFKLPRYKREEKQMVETSMSLLEKVGLADSRYEK
                     SSSLPYGQQRRLEIARALATDPSILLLDEPAAGMNPKESEDLTVFIKEIRDEFDLTVF
                     LIEHHMQVVMGISDRIYVLDYGITIAQGNPYEIQNNQRVIVAYLGVDEDA"
     gene            124555..125268
                     /locus_tag="PKU88_00640"
     CDS             124555..125268
                     /locus_tag="PKU88_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX67830.1"
                     /translation="MLKIDNLVVAYGGIEALKGISLEVEEGKIITLIGANGAGKSTTL
                     RSIMGLVKPKQGEITYMGKNLLSMKTQEMVRHGITLVPEGRRIFPNLSVLENLRVGAF
                     ARNDEKGIKEDIEMVYGLFPILKERSWQTAGTLSGGEQQMLAVGRALMSRPKLMMMDE
                     PSLGLAPLIVKEIFNIIKEVNKQGVTILLIEQNANAALHIADLGYVMETGRITLKGTG
                     AELLANPDIKKAYLGAEAH"
     gene            complement(125436..126680)
                     /locus_tag="PKU88_00645"
     CDS             complement(125436..126680)
                     /locus_tag="PKU88_00645"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HPX67831.1"
                     /translation="MELTVQRARNIINALKNGVVPDCDLELLCVGRDREIKEFQRCFQ
                     VVSEGSGTVKFLNGEYGSGKSFFMNVIKQAAVKNDFIVSKVQVDRGFRFNNLEHLYYH
                     IMHNLALNIAEGSAASFESIFDLWISKLQESSNKDSAAAEINHVISSLNRYNMSFARA
                     FLAYIRARIEKNTVLSDTVASWLMGEKNIPASIKAKFDVVGSVDRLNSIDFLKAFVKL
                     IKLLGYSGLIILIDELELIMNERIDIRRVSYENLRNIIDVCCSGELGSCMFVFAATND
                     FFENPEKGIKTYEALSQRLGDAIDKSNSALYDVRQPVIRLHKLNTEEFQELTDRVINI
                     YKMLYNFVPANSNESIRSWTFISLRKFGGKLAELSIREYLTKLIEVLDIMEQHPENKL
                     FTSELKAVKKGDTEVYINRNRG"
     gene            127104..128750
                     /gene="abc-f"
                     /locus_tag="PKU88_00650"
     CDS             127104..128750
                     /gene="abc-f"
                     /locus_tag="PKU88_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HPX67832.1"
                     /translation="MLLLEINGLKKYYVERLILDIKDFKAYYGERIGIVGANGAGKTT
                     LLDIIAGRNIPEEGSIKLYGEISYITQLDLEAASEVDNRMAKEFGLNFNSLDTASGGE
                     KTRFKIAGSLSENNSILLADEPASNLDIQGIELLEKKLASYKGLILIVSHDRELLDKL
                     CSKIVEIEGGELKQYSGNYSQYKLQKEMEFKRAQFEYDQYIDSKRALEETVKEKKNRA
                     ASMKKAPSRMGNSEARLHKTGNQKAKANLDKAIKAMETRIRKLEKKEKPKDQQHAIID
                     IQGAEKPVSRVLISGSGVSKSFGSKELFREAEFEVLNGSKTALLGGNGTGKSTLLNMI
                     MGGDESIKLANGVKLGYFSQGTDILKDDETILENVMKESIYPEHFVRTILARLLFRRD
                     DVYKKAGKLSGGERVRTCFAKIFCSDANVIILDEPTNYLDISSMEAVENAMRLHEGTI
                     FFASHDRKLISSTADRIIYIEGRRLNTFEGSYGEYVEFRRRAADSGETERKRQKLLLD
                     TRLSEILSRLSMPGKDDDVEQLDMEYKRVLAEIRQLKSGR"
     gene            128827..129482
                     /locus_tag="PKU88_00655"
                     /pseudo
     CDS             128827..129482
                     /locus_tag="PKU88_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
     gene            129581..130909
                     /locus_tag="PKU88_00660"
     CDS             129581..130909
                     /locus_tag="PKU88_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPX67833.1"
                     /translation="MDVAAALIVTFMLLMLSVYRGINILYPLLIGLIIFITVAYKRGY
                     KPKEIIGMILKGIRKSMLIISIMMLIGAIMSVWRASGTVAYVVYYGIRFVDPNLFILY
                     AFLLSCVVSFLLGTSFGTVGTIGTILMVMVKGGDININIVAGAIMSGIYFGDRCSPMS
                     SSANLVSVLTETDLYTNIKNMFRTTAVPFLIASIIYGILSFQNPLQLGENRIITGIVQ
                     TYNLNWVVSMPAIIILVLAFFKVDIKISMAVSILCGAAVAVFFQKETIGDVLKYIIMG
                     YSRPEQSFLGDIMAGGGIISMINVVLIVLIAFSLSGVLEGASLLKFAGNLIIKLGKKV
                     GIFAAVILTSIIAGAFGGSQTLAVMLTYQLVRNMYDSAGIDKYELAVDLENTAIVMAP
                     LIPWNIAGAVPAAALTADNSYVLYSFYLYLIPLANLVMKKFEIIKRHKMA"
     gene            130939..131208
                     /locus_tag="PKU88_00665"
     CDS             130939..131208
                     /locus_tag="PKU88_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67834.1"
                     /translation="MDVILNALQSVLSMAVLTMLGYFLTLVIFIPSLMTKVFVIQSAM
                     PVIAQSAIVAAAFGGDQYYATYMVTITSILSVVFIPAYMVLLNGV"
     gene            131237..132763
                     /locus_tag="PKU88_00670"
     CDS             131237..132763
                     /locus_tag="PKU88_00670"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPX67835.1"
                     /translation="MISRVKRKSTIFIITALFILIAGGCSVKQESPNIPVPAVVPSPT
                     AKGTDVNQYIIDAVLDTANKELTAEQQVIYVNNDDVELSELYFHIYANAFRKQETAPF
                     LFDDFSMAYSRGFKPGYTEIEAIEFSKGQNRNALEYSLQGVGETILRIKLPEPLKPEG
                     SVNLRFKYKVIIPPAGERFGYGDANINMGNWYPVAAVFDDEGWNLDKYYSIGDPFYSD
                     VSDYIVSIKAPEEYIIAASGSLAEERIEGGYKNWKFHGANMRDFAFVASNNFRVAEDK
                     VGNTIVKSYYYKDHEKRGKEVLDIGKRSIEVFNSAYGEYPYPTYSIVETEFPSGMEYP
                     GLVYISTKYYDNDSGADIFMYTTVHETAHQWWYGVVGNDQIDEAWLDEGFATYSEGVF
                     TEMEYGEGNGDLYYKYLEESAKESIRAKAYDGVILKPLSRYENWDDYGPAVYTGGAVL
                     LNEIRKQVGDHAFFIILQTYYDEYSFKIATTEDFLRICENVGNAEFDDLFHKHLSSVK
                     "
     gene            133157..134452
                     /locus_tag="PKU88_00675"
     CDS             133157..134452
                     /locus_tag="PKU88_00675"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPX67836.1"
                     /translation="MIKKKVGSITLAVGLITVGALLFAQNFMDIPVKDIYKYWPVLLI
                     GLGLEMILYMVLYGRNKTEVKLSVDGLCIVFIIILGIISNGIRFIDINIPGRHFFGFN
                     GSSMFEGFKYRTEISEKYSKDNISGGFNVKTLKVTNDFGEIKILPAGGNTIRLEAEVM
                     VKCNDENKAREYARNAVEIIEGEVTELSPKIPPNWNKNDHARAQVDFVVYVPKEISLI
                     ARGSFGDIEAEGIGGECRIENKNGEITASNIGGDVDIDNSFGDIEVSNIGGKADISNH
                     NGEISADNIGGSAVIENHFGDIEAEKVGGDLKVVNNNGRIYVKEVGGKADLVNAFGDI
                     GIKRADGGITVENKNGRIEAEDVAGNAQIKNAFGDIYYKSASADNGDIYARTKFGSIN
                     CNKPLQIKKEGQDTVAQGRLGTGQYKIELETNNGSIVIE"
     assembly_gap    134486..134585
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            134743..135399
                     /locus_tag="PKU88_00680"
     CDS             134743..135399
                     /locus_tag="PKU88_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPX67837.1"
                     /translation="MYKDIKAVIFDVDGTLIDSMWIWKQVDIEFLGKRGIPLPERLQM
                     EIEGMSYSETAVYFKERFNLPESLEEIKEEWRLMAEDYYKCHIQLKSGAKEFLKLLYD
                     KGLTLGIATSNSRELVDCMLANHGIRKYFSNIRTSCEVDKGKPYPDVYLKVAEDMAID
                     PCRCLVFEDTLSGVMAAKSAGMRVFAMADEISLESKDKLIELAEGYIHSFNEVMEYFA
                     "
     gene            135498..136085
                     /locus_tag="PKU88_00685"
     CDS             135498..136085
                     /locus_tag="PKU88_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPX67838.1"
                     /translation="MRDEEVVSYIVKSKTELFSVIIDRYQSKVFSTAFHYTHDYEEAR
                     DLTQEIFIKLYNNLQKYKGKASFSTWLYRIAVNRCIDWARKKKPQTVSAICDGSEEEL
                     DIYDTIADSGGGPEEDLIKQENAVYLRKLVESLPEIYKTVIILYYFEDFSPQEISEIT
                     GVPKRTIDTRLFRGKNLLKLKMEELICGGEQNVLR"
     gene            136072..136575
                     /locus_tag="PKU88_00690"
     CDS             136072..136575
                     /locus_tag="PKU88_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPX67839.1"
                     /translation="MCCDENRGMLHEYAAGKLSELESYAVARHLKSCDRCRRELAEIK
                     ALKVFLKSGFQESIIPSVDLKSSIMASVDLKRYKKSYRNTLGEMANLGMSLVAAGLIL
                     LLLNTVPKDDLVRVQNELYNTGENITQKISQPFSSINERIDKLTNSITQLDGIMGRIE
                     REKEGGK"
     gene            136577..137272
                     /locus_tag="PKU88_00695"
     CDS             136577..137272
                     /locus_tag="PKU88_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HPX67840.1"
                     /translation="MNCKYHKESEAKFICDKCKQPICEECSVDVNGNKICSSCIQKSL
                     FSENRQYQKPGFFENLAFFCFAVVPGAAQMYMGLFKRGFQLMFGFISAIVLFSYLNTE
                     GMIMLIIIPTWFFSFFDSYAMRRRIKKGEEVEDSVIFDYNILIENKRIVGMVMLFLGI
                     LGVVNAFGRSVFQLVFGGDLYWSIRRSIMPIALIGAGVYMLSKSKKKEEAKNTEDIIE
                     IEEESEEEIEAAE"
     gene            137493..137681
                     /locus_tag="PKU88_00700"
     CDS             137493..137681
                     /locus_tag="PKU88_00700"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HPX67841.1"
                     /translation="MQAPTVQLGKPDKAENFDLYSVDGVDAYIKKGTRIRNDQIHIFL
                     RKFLWIKDLAVDGVRVDY"
     gene            137753..137829
                     /locus_tag="PKU88_00705"
     tRNA            137753..137829
                     /locus_tag="PKU88_00705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137787..137789,aa:Arg,seq:acg)
     gene            complement(137860..139062)
                     /locus_tag="PKU88_00710"
     CDS             complement(137860..139062)
                     /locus_tag="PKU88_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX67842.1"
                     /translation="MIQRKEYLNKLIEWREKQVIKVVTGVRRCGKSTLFSLYIDYLKK
                     TGVSEEQIILINLEDIEYENLLDYRPLYDYIKARIVKGKYTYVFIDEVQQCKSFEKAV
                     DSLFLKENVDVYITGSNATMLSGELATLLSGRYIEISMLPLSFSEYLEFAKGRDVKST
                     FDEYLKFGSFPYVAVLNKSDAVVRTYIDGIYNTILIKDVAARERIKDISVLKSIVKFL
                     CSNIGSPVSVKKISDAINASGRKISVNTVENYLRALYQSYMFYKVDRYDIKGKQQLKT
                     LGKYYIVDTGLRNLLLSSSAADIGHLLENVIYLELIRRGYKVNIGKLAEKEIDFVAGN
                     TEGTEYYQVSASVLDRNTLQRELEPLQKIPDHHPKFLLTLDDIMPNANHDGIRQINAI
                     DWLIKKKP"
     gene            139281..139685
                     /locus_tag="PKU88_00715"
     CDS             139281..139685
                     /locus_tag="PKU88_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPX67843.1"
                     /translation="MMSKKNYKMIGILICIAAIGFGVFYFINIAPANRVLYEVRNAEI
                     KDIDLNEIADGKYTGEFSYNNTRCMVEVIVNNHRIEAINVLECGTPEYGKKAEAVIDR
                     IIDKQKTNVDAVSGATTTSKALLKAVEAALSK"
     gene            complement(139708..139833)
                     /locus_tag="PKU88_00720"
     CDS             complement(139708..139833)
                     /locus_tag="PKU88_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67844.1"
                     /translation="MAFISSVMVIVRWFKGKKKKESDSGITEESNEQICDNNESA"
BASE COUNT        44009 a        24271 c        32593 g        39041 t
ORIGIN      
        1 atacgcagca tgtttgacat gagaaatttc accgatttag tttacaggta atatatctaa
       61 acagggcgtt tatgccgcga aagcctattt acatggggtg gtaccctctg gtaaaatctt
      121 ggcagcggaa aggcgcaaag aatataggct aattccgcct aaaaataggt gtccagaggg
      181 tacaggggtc cccatgtcaa taggatcgtg gaataaatgc gaccaaattg acaaaaacat
      241 ttttagtaaa aatttgtatg aaggtaagtt gccaacgttt acttgacagt aacttcaagc
      301 ttcataatat tgacttggaa actatattct gttaggatga aatttgaata aaaatactta
      361 tttaatttgc acagcaatat tctgtcagta cgatattgct gtgaagatgg aggtcggtgc
      421 cctaatgaaa tacgatgtta taatagtcgg cgcagggcca agcggaatat ttgcagctta
      481 tgagcttatg caatatgatg caggtattaa ggttgctata ctggaaaagg ggaatgagct
      541 ggaaaaaaga gcatgtccta tagataataa aaaaataaaa tcatgcatta actgtcccac
      601 ctgcagtatt atgaacggat tcggcggagc aggaggaatg tctgacggaa aatacaacat
      661 aaccaataat tttgggggag agctttacaa atacataggc aggaaagaag ctcttgacct
      721 tatgtactac gtggatgagg taaattgcaa aatgggcggc gaaggcgcaa tgctttattc
      781 tacggagaac agcagcctta gaacgcaggc attgcgacac gatctccatt tgctggatgc
      841 caaggtgcgt catatgggaa cggacaaaaa cctcgtaata atgaaaaatc tgtatgagta
      901 cctaatggat aaggtagaaa taatatttaa taatgaagta tcggatattg aaaaacaagg
      961 agataccttt atcgtatcag cagaaaaagg agaatattct tgcagtcatt tgattctggc
     1021 tgccggacgt tcaggttcaa aatggatttc gcagatttgt gaaaagctgg ggataaaaac
     1081 ggaaagcaac agagtggaca ttggtgtgcg ggttgaaatt ccggcttcaa tatttgagca
     1141 cattaccagc caggtttatg aaagcaagat tgtatatagg acagagaaat acaatgattt
     1201 ggtaagaacc ttctgcatga atccccatgg aaaagtggtc actgaaaata cgaacggaat
     1261 tgtaacagta aacggacaca gttattcaga ccccaagcac cataccgaga atacgaattt
     1321 tgcgctgctg gtttccaaca gatttacaga gccctttaag aacagcaatg agtatggtga
     1381 atcaatagca aagctcagca atatgctggg cggaggagta ttggtccaga gattcggtga
     1441 cttggtaaaa ggacgcagat ccagtgaaag gcgtatggaa aaaagctttc tgcgcccgac
     1501 actgaatgct acaccgggag atttgagcct tgtcattcct aaaagacagc tggatgatat
     1561 catagaaatg atttatgcat tggacaaaat tgctccggga acagcaaatg aagatacgct
     1621 gctgtatggt gtggaagtca aattttataa ttcccaggta gaggttgaca ataacctgga
     1681 gacaataatc aaagggctgt atattttggg tgacagctca ggagtaacac attcgttatc
     1741 ccaggcttca gcaagcggag tatatgttgc ccgtttgctg ggggaaaaat actgcaagca
     1801 cagctaagct cagagaaatt aattaaagag gtgttatata tgtcatcatt ggtaatagta
     1861 ggagcacaat ggggagacga aggaaaggga aagctcacgg attatcttgc cgaaaaagcg
     1921 gatgtagttg taagatatca gggaggcaat aatgccggac atactgtaga ggttgataat
     1981 gaagtatata aactgcagct tattccttcg gggatattgt acgctgataa aatgtgcatc
     2041 ataggcaatg gggttgttat tgatcctgaa gcatttctta atgagatcag aaatctggaa
     2101 agcagaggca taagtacggg aaatctcagg ataagcgaca gagcgcatgt attattcccg
     2161 tatcataaga agattgatga gctttccgag cttaaaagag ggagcgatga tctggggaca
     2221 accaaaaaag gaataggccc ctgttatgcg gacaaaacag aacgaatcgg gataagagtc
     2281 tgcgatttat tgaaaaaaga ggttctcgca aagaagctca aaaccaattt ggagctgaag
     2341 aatgcatatc taaagaaaat atacgatttt gaaggctatt catatgaaga aatgctgtcc
     2401 aaatacctgg actatgcgga acaggtaagg ccttttgtcg aagatacatc ggtattgatt
     2461 tacgatgcaa ttaaagcagg aaagaaagtg ctcttcgaag gagcacaggg aacgctgctg
     2521 gatctggact tcggaacata tccttatgtt acttcctccc atcctattgc agggggagta
     2581 tgtataggcg caggagtcgg gcctgccctc ataggcaagg tcataggagt tgtaaaagca
     2641 tatacaacca gagtaggaaa gggtcctttc cccacagagc tttttgatga gacaggagaa
     2701 tatatcagga ctacgggcaa ggaattcggc actgtaacag gaaggccaag gagatgcggc
     2761 tggtttgacg ccgttatagt cagatacgct gcgagaatca gcggacttac aggtattgcc
     2821 ataaccaagc ttgatacact gtcaaatatt ccgactatta aaatgtgcgt aggctatagg
     2881 ctggatggaa aaataatcag ggattttcct gcaagcttgg aggatctgga aaattgtgaa
     2941 ccgatatatg aagagtttga aggctgggga gatatcggtg atattaccca atacaacaag
     3001 ctgcccacaa gtgttctgcg ctatctggaa agaatcgaag aaatctgcgg aacaaagata
     3061 tcgattatct ctgtaggccc aaagagaaat caaacaataa tgattgataa gttttaggta
     3121 tgcatgtgat ataattgaaa caacagatgc agaaaatgta tattgacaaa ttgattctga
     3181 cattgtagaa tgtataaatg tgagccgcaa aaattgctca catcgtgact cactagctca
     3241 gtcggtagag cacatgactt ttaatcatgg tgtccggagt tcgattctcc ggtgagtcac
     3301 cattaatggc ccgttggtca agcggtcaag acatcgccct ttcacggcgg taacgggggt
     3361 tcgattcccc cacgggtcac cataaaatgg gggcatagct cagctgggag agcatctgcc
     3421 ttacaagcag agggtcatag gttcgagccc tattgtcccc accagctttt tccgcttcgc
     3481 ggaaaaagac taacattcct tgatttaatg cgtagcacat gcggagcaat ttatgacccg
     3541 cagagcgtaa aagtaaaaag accgacattc attgattaaa gccgtagcat ttgcggaggc
     3601 aaatacttgg cccagtagtt cagttggtta gaatgccagc ctgtcacgct ggaggtcgac
     3661 ggttcgagcc cgttctgggt cgccatatgc tggtgtagct caataggcag agcagctgac
     3721 ttgtaatcag caggttgatg gttcaattcc gttcaccagc tccataagaa attttaaggg
     3781 gttcgtaaaa ctatttaaga taataaatag ttagcgatac cccttttctt attgattgtt
     3841 tttgtatata atataattag acatttatct aaatatctaa ttatattaat aaaagaggcg
     3901 atgaaattgt ccatactcaa tatgccgttt aaagccctgt ctgatccaag caggcgaaaa
     3961 ataatccaga tgctgaaggg aaaagatatg actgccggag aaatagctga caattttcaa
     4021 atgtcaaaac ccagcatatc tcatcatctt agcattctga agcaggctga tctggttaca
     4081 gacgaaagga gaggccagaa tatttactat tcattgaata ctacggtgtt tcaggaagtg
     4141 gtcaattggt tttttaacat gacagataga agcggagggt ttgaaaatga aaaagattga
     4201 tttaaacatt aaaaaagaac tgccgatcat actcataatt gtcttgatgt ttgcagcaag
     4261 tatatacttt tatccccaat taccggataa aatccctaca cattggaatt ttaaaggaga
     4321 aatagacggg tactcgggaa agctgacggg agcattcctt atgcccgtaa tgaatataat
     4381 aatgtacggg ctctttatat ttatacctgc tattgatccc aaaaaggaaa actataaatt
     4441 atttgaaagc acctatatat atttcagata ccttttccac atcttctttt tgggtataca
     4501 cggcctgatc atagcatcag ccctgggcta caatgttgat acgggaaggc tggttacttt
     4561 ggcagtttca ctgctgctca tgctcatggg caatgtgatg ggaaggctga agcacaatta
     4621 ctttgtcggt ataaaaaccc cctggacctt ggctaatgaa gaggtatgga gaaaaaccca
     4681 caggcttggg gctatccttt ggacagccgg cggtttggca ggagtgatca tgtcactgct
     4741 taggctgagt atgggatggg catttatagc agtaattgcg gttatgactt ttatacccat
     4801 agtgtattca tatgtaatat acagcaaatt caagaaataa gaaacaactt tgcattatgc
     4861 agccttcggt tattttgatt tgcacttgat atttatactg ctatgtttaa taataaagat
     4921 aataaaatgc tgtaatccat aaaataatat aatatgctat taataataca aggaggagag
     4981 tcaacatgaa aatcgtagta cttgacggat atacattaaa ccccggggat ttaagctggg
     5041 acggacttga aaaacttggg gaaataacgg tatatgaccg cgtatcatat gatgaaaaag
     5101 atgaagatat ggtagcggaa agcgttggcg atgcagaaat tgtttttact aacaagactc
     5161 ctgttacaag aagggcaatg aagaaaatgc ccaacttaag atttatcgga gtattggcaa
     5221 caggatataa tattgttgat gtggttgccg ccaaggaaaa gggagtagtt gttacaaata
     5281 ttccaaccta tggtacggat tcagtaggtc agtttgcaat tgcgttgctt cttgaaatgt
     5341 gccatcatgt cggtgcacac agcgactcgg tattaagggg agagtgggca aataaccctg
     5401 attggtgcta ctggaacttt cctttaatcg aactggccgg gaaaacaatg ggtataatag
     5461 gatatgggag aatcggccag acaacgggca ggattgccca ggcattgggc atgagggtta
     5521 ttgcagctga ttcgtataag aatcccgcat tagagagtga tacaatgaaa tatgtcgaac
     5581 ttgatgagct gtttgccaaa tctgatgtga ttgctttgca ctgtccgttg tttgaaagta
     5641 cgaagggaat aataaacagg gaaaatattg caaagatgaa ggatggagtt atgattataa
     5701 ataactcaag aggcccgttg attgttgaag aagaccttgc agaagcttta aacagcggca
     5761 aagtagccgg agcggctgtg gatgttgtca tgactgagcc gataaagcct gataatccat
     5821 tgttaaaggc aaagaactgc attataactc ctcatatttc atgggcacca aaagagtcaa
     5881 ggaaacgtct gatggatatt gcagtaaaca atctggagaa attcctagca ggtacaccgg
     5941 taaatgtagt taatccataa gaaagattaa gagaaaacac agcacttata tacaaatctt
     6001 tattatatgt cgatattttt aaaagatgtc gacatataat ctattgacaa cgaaaaatat
     6061 atatgtaaaa atatagttag acaaaaagac ggtatctaac tgacattaga caccgtcttg
     6121 tgtgaatccg atggttcaca cgtgctttat gcatactata agaatatttg ataagctgta
     6181 ttttgtcaat atccatcgga gaaaggaagg taccacaatg gcgttttctc aatcagtagt
     6241 ggatgcggca tggatacgtt caggtggtaa gtgcgagtgt acacgttcat cacatggcca
     6301 tagtattaga tgtaataaaa ctctctccta cgataaccgt ggtaaagatg gaaattgggg
     6361 tgcatgggaa gcacatcata agatttcagt ggctgcaggt ggcagcgaca atttgtccaa
     6421 ttgtgaaatt ctttgtatag attgccacaa gaagacaggg agttatggtg gataattagt
     6481 tatttgtatg tatagctgaa taaggatagg ggaaacccta tccttgaagc ttataggaga
     6541 gtgaaagaat ggataaatta aagccttata atgatttgac accgataagt caattggcta
     6601 ttgaagaaaa caagccatta ttaaagcttg cggaagatac cagggtagct attgaaatac
     6661 tgaattatga aatcaattca cttccatctc catctataat gattgatacg ttgactttgc
     6721 aggaggcaaa ggtaagcagc catattgaga acattgtcac gacaaacgat gatctgtata
     6781 aaggaattgt atttgataat tatactgcag aagcaaaaga agttacgaat tacaaaactg
     6841 cattgtttat cggttttgaa aggttgaagg aaaaaggtgt gttgtctatt ggcgatattg
     6901 aagccataaa tgaaccagtg aataaaaaga caaaaggcat aagggtcaac atgccggagt
     6961 ttgagaacag ccttaccagg attgcaaaca aacggggcaa tgaaattgaa gtgctctata
     7021 cccctcctca tggtaaggag ctgctccaaa agctgcttat tgatatgctt gaatatttat
     7081 atgatgatga aacatacaca attcatcctt taataaaaat tgcactggca cattaccagt
     7141 ttgaatgtat acaccctttc tatgacggta atggaagaac aggccggata cttaatgtgc
     7201 ttatgttatg ccaaaagaga tatctggact atcctatttt atttgccagc tcatatataa
     7261 tcaagaacaa gaatgagtat tatgagttat tacagacatg caggcagaag gataattata
     7321 catctattat agaatatatg ctgaattctt tcaaagtaac ggcggaagac acactgaaca
     7381 aggtaaacag cattaaaaag cttatagaaa aatattcaga ccaagaattc ctggagacat
     7441 taaagggaca aaaaaaagta ttgagtcagg tcatggagct gatttttaag aaagtatatg
     7501 taaggattga agatattgtt gagatgggaa tacatagaca gacggcatcg gcatatttga
     7561 agcagcttgt tgaaaaaggc ctgcttcagg aagaaaaggt gcagcgggat aaagtgttta
     7621 agaatattga actgttaaaa ttgtttgaag gagaacaaat atgataaacc aagcagatat
     7681 aaatgcaaaa gtaaatcttg ttaccaaaat tgccaacaag cttagaggcc cctatacatc
     7741 tgagcagtac ggcagtgtta ttatacctat gtcaatctta agaaggtttg actgtgtgct
     7801 tgagggacag aacagcaaaa taaaattaat actggaaaag tttaaatcgt tgaatgtaga
     7861 tgaaaaactg attgaacagg ctgtacagaa ggaatgcgga atacccttct acaacaagaa
     7921 gtgtgtcacc tttgaagact taatcagcga tagtgataat atcaatgcaa attttatgga
     7981 attcctgacc tcctattctc caagcatagc gaatataatt aaaggtttga agttcaaaga
     8041 agaagttgaa ttcatgataa aaaagggaat actgtttgag gttgtgaagg ctttttcgga
     8101 agttgacttt catcctgata ctaccgacca attatctatg ggatacatct ttgaggaaat
     8161 aatcagaact tacaagtcca atgccgaagc gggtgatcac tatactccca gggaagtaat
     8221 tgagctttgt gttgaattaa tggtagccga gaatattgat gaattaaaaa aggaagggaa
     8281 gataataagg gtatatgacg gctgctgcgg cacagggggg atgctcacag taaccaagag
     8341 gaaacttggt gaattgcttg gcagtgatga aagagtccgt cttttcggac aggacaacaa
     8401 tcctgaagca tatgcaatat gctgtgcaga gatgctgatg ctgggagagg atgttaaaaa
     8461 tatatatgag ggaaataccc tgactgaaga ccacttaaaa ggtgagaagt ttgatattct
     8521 gattaccaat cctccctttg gagtggagtg gaaaacagaa aaagccattg tagaaaaaga
     8581 gctaaacagt ccaaataacc gttttgttgc aggtacgcca agagtcagcg acggtcagct
     8641 gctgttcctg caaaaccttc tggcaaaagc agagaagaac gccaggattg ctataatatt
     8701 gaatggatct ccgctttttt ccggcgatgc cgaaagcgga gaaagtgaaa tccgccgcta
     8761 tgtgctggag aacaatcttc ttgaagcaat tgtcgctttg ccggatcaaa tgttttataa
     8821 cacaggtatt ttttcatata tctggatatt atcaagaaac aaatcagaga agcgggataa
     8881 aaaggttcag cttataaatg ccgtagattt ttgggagccc cagcagccga aatccctcgg
     8941 taataagcgc aaatttatat ccccacataa cagaaaaaaa atagtggagc tttacagtaa
     9001 atttgaggag aatgagtatt caagaaaatt tgacaatatc gattttggat atcataaggt
     9061 tacaatagaa aggcccctaa gggatgaaac cggtgctccg attataaaaa aaggaaaagt
     9121 gcaggcggat ggagctttgc gtgatacgga aaatataccc cttaaggaag atatcaatga
     9181 atacttcaat agggaggtta agccttatgc tccggatgcc tggatagatc atgacaaaat
     9241 aaaggttgga tatgagatac cttttacccg gtatttctat aaatatgttc cacccaggaa
     9301 gtcaggggat atattatctg agataattga gattgaggat gatataaaag gtactattga
     9361 gactctgctg gggaggagag agtaatgcat aattcacaat gcataattta taatgggaag
     9421 ataagtatta atgaagtaaa gacaaagtat ttattcaaaa tagtaaatgg ggctacacct
     9481 tcgagcagta atattgaata ttgggatgga aatattactt ggataacgcc tgcagatatg
     9541 agtaaatgta ttagtaatat catcatagat tcagacagaa aaataactga agaaggattg
     9601 aattcatgcg gcacatcatt agttcctgct ggtagtatta tattatcaac acgtgcacct
     9661 attggatcgg tttgtatagc tggtgttgag ctatgtacta atcaaggatg caaggcattg
     9721 gttgcaaagg ataatgttaa tgaacgtttt tatttttatt atttattgat atctgctgaa
     9781 ctgctgaatt ctttaggcag aggtactaca tttttagaat tatccggtag cgacctaggg
     9841 aattttttgc tgccctttcc gaagaaagct aagcaagacg aaatagctaa ctatctcgat
     9901 caaaagtgtg cggaaattga tagagttgtt gctgcaaagc agaagcagaa tgaactttta
     9961 aaagaatata aacagtcaat catatatgaa gcggtaacaa aagggcttga caaaaatgta
    10021 aaatataagg atagtggtgt tgaatggatt ggggagatac cggaggattg ggaaaagtgg
    10081 aaaataaaat acgtttgtga tgttgataaa aatagattat cagaggatac agatccgaat
    10141 tatttattta agtatataga tatcagcagt gtcacagaaa tgggaggtat tggagagact
    10201 gttgaaatga cttttgatac aagcccttca agagcaagaa tgattgtgtc aaaaggagat
    10261 actataatat cgactgtgag aacatattta aaggcgatag cttttattga agagagcaat
    10321 aaatatattt gctcaaccgg tttcgcagta ttaacatcaa agaagggact attaccgagg
    10381 tttttatttt atcaggccca aagcgaatat tttattcagg aggttgttag cagatcagtt
    10441 ggggtaagct atccggcaat aacttcaagt gcattaagca atattagttt ttttgtacct
    10501 tcgcttccta ctcagcagcg tattgccgat tacctggatc aaaaatgctg cgagatagac
    10561 cgaataataa agagcaatga tgatatgatt acaaagctta aggaatacag acaaagtgtt
    10621 atatacgaag cggtgacagg gaaaattgaa atgtgattga aggtgaaaag cttggcagga
    10681 gaggttttaa gagaaaaccc attcagagat gagatatata attatctttt aagtatcgga
    10741 tacaaagaga gcataaaagc ggactatgac tacatgaatg caattgatag ggcaaagctc
    10801 tttgaatttc tggagaactc ccagaaggag cagcttgaaa gctttaagga tacttacaaa
    10861 gatagctgga tggaacggtt tattaagcta ttgaatgata aaatcaataa cgaaggcctg
    10921 cttgcagctt tgaagggcaa gctggaggac tatcaaagca gcaccggatt ttctcttgca
    10981 ttttttggtt caaatattga aagtatgaca tataacaagg agctttacga aaaaaatatt
    11041 ttctctgcct gccgggaatt tgcctatgaa gacaagcagg atagctttcg agttgatatt
    11101 gcaatctttt taaatggtat acccataata atgattgaac tgaagaagca gactacagga
    11161 cagaaggcgg gctttgaagg aacaagtcag ttcagaaaca cgagaaatcc cgacgagctt
    11221 attttcggtt ttaacaagag aaccttggtg tattttgctg ttgatgaatt tgaagccttt
    11281 gctgctaccc gccttaatag aaagaacact gtgtttgttc ccttcaacag gggatcctct
    11341 gatgaagggg caggaaaccc aattgtagaa ggcaagcact gtactaacta tgtatgggaa
    11401 gaaattcttc ggaaaaatat gcttttaaag attattcgtg agttcatgtt cattgatgaa
    11461 gacggagaaa tgatatttcc cagatatcat cagctggatg cagtgctgaa gcttgaagcg
    11521 gatgtgaagc aaaacggtat aggcggaaga taccttatct ggcatagtgc tggaagcggc
    11581 aaaacaaaga ctattgcatg gctttccaaa aggctgatta atatacagga tatacatacc
    11641 gtaattgtaa tcagcgaccg taccgttatt gacagccaat taggcatcga aatacagaca
    11701 cttgacggaa agaagggtgt agcaaagtgg atagacacta catcggcaga gcttctcaag
    11761 atgcttaggt cgggaggcta tatcgttatt accacacttc agaaattcgg aaatattctt
    11821 gatgagctga aaaacatccc caacaggaat tatgcaatta taattgatga ggcgcactcg
    11881 tcaacaggcg gaaaatcatt gtcgaagacg gcggagaccc tggatggaaa atccctgaag
    11941 gattcgatac ttatagacga aacatacgag gaactggagg ataatcagaa tcgtattctg
    12001 aagcaaggga gcaaaatcaa gtcaacaaaa aacataagct attttgcttt tactgctact
    12061 ccaaagaccg aaacagtaga gctgtttggt acacgcactg ccatcgggaa aaaatacttc
    12121 cacaaatact ctatgaaaca ggctattcag gaaggattta tattgaaccc catagtatgt
    12181 tatacaattt ataatgagaa atttgaactg gcaaaaaaga aggatgatcc taccgaatac
    12241 gaaaccgcta aagcttccgc cgccattttg aatcatatta ccagttcagc cgaggttatt
    12301 gattccaaaa cggaaataat tatggaggat tttttgatta aacgtataaa ctggcttgac
    12361 ggaaaggcaa aggctatgat tattaccccc tcccgcaagc atgcggtttg ttacaagctg
    12421 gcggttgata agtacttgat aaaaaagggg tataatataa agacaatagc agcatttacg
    12481 ggaacaatag aacttaacgg aatcaagtat accgaagaaa atatgaatcc gggttttaag
    12541 gaaaaggaca taaaggaaat aataaaaaac aacgatgatg tgagagtcat aatcgttgca
    12601 gacaaattgc agacaggctt tgacgagaaa aaactatgta tcctatatgt tgataaaaag
    12661 ctcaatagtg ctgtaaaagc agttcagact ctgtccagaa tcaacagaat ctgtaaaggt
    12721 aaaaagacct ttatacttga ttttgtgaat aagacggaag aaataaaagc atatttcgaa
    12781 cagtactacg ggggggagct gttcttgccc tctgaaaatg aaacagatcc taatatactt
    12841 tttacaaagc gggacagaat tctgaattat tttgtattta caattgaaca ggcagaatac
    12901 gcgtatagtc taataaatga caatgcaaga cactcaggtg aattgacatc aatatttgca
    12961 aaagctaaag aaaaatataa tgagatggat aaagacagaa aaaaattatt tcttgccgaa
    13021 atttcaaaat ttgtaaagtt gttttattat gtttcaattg tatataacat ttggaacagt
    13081 gatatgaaac gatttgcagt gttccttgat gctctttaca atgtgcttta cgaaaagtcc
    13141 gacgatacaa gaatcaatcc gggggaattg gttgagcttg ttgaatactc agttaaaaag
    13201 gcttcggagg aagaagatct gcttgtaaat gttgcagatt attcgctgga aagggtgccg
    13261 acggaatctt atgttaaaga ggaaagctat tctttaattg atgagataat atatagaata
    13321 aatgctcgtt acggcaaata tgaaaatgct catcaggaat tggttgagat tgtgaatact
    13381 ttaagcagtg acaatgaaat gataataaat gtaagagaca gttcacccag tgcttatgag
    13441 gcagaagcaa tggataaaat cggacagata tttgtaaatg ggattttgtc tgcggcagaa
    13501 gaacgctcac agttttatgc acaaatcagc agtgataaag atgtcataag atctcttgca
    13561 aaggcagtta taagaaggat tcaggatgaa cttaaagcag gataggggat aacaagaccg
    13621 tactggcatt tgttgcacag tacggtactt gcttttgagt ctttatgaaa tacctttcat
    13681 tataagacga atatcttttc ccacaaactt gcacgcttca aagttaccaa taattcttga
    13741 aactattctc tcggagtatg tgctttgaaa atctacgccg cttaagtttg tggatataat
    13801 cattttcttc atattgacta ttcttttatt tagtatattg tatatagcct gattggaaaa
    13861 ctgagtggaa agctcagttc ccaaatcatc cattattaaa aggtcacaat tgtatatttc
    13921 atttaaataa tgcgcatagt tttcttcttt atcaaaatca aacttatatt ttcgcatcgt
    13981 atcaatcaaa tccggaacag tcatataaat aacacttcta cctttatcca acagatcctt
    14041 ggctatcgag ttgcatagaa atgtctttcc taatccggga ctgccgataa aaaacagatt
    14101 tttattatgt tcatcaaatt gctcaacaaa gtcgatgcat tttttatata tctcctgcat
    14161 gtttttcaaa ggggatatgc cattttcgtc aggatcatcg gaataataat cccaacggaa
    14221 ttgctcaaaa ttctcttttt cgagtataaa acttaaattg gaatactcat agttcaagta
    14281 tatttccctc tgtttataac atttgcattt ttggtaattg acaaaacctg tatccttgca
    14341 caacttacat ttatatatgg aatcaagata atcaattgga tacttattag ctgatagtat
    14401 ttctgctttt tctactttca aatcaagctg cttattcttc agtttatcta ttaaaacttc
    14461 agcagtatta gatttgctaa gtatagctct agttatgtca atacttagct tcaccatttc
    14521 atcttctatt tctttaagcc taggtaattt atcatataga gttttcttac gtgcttctat
    14581 ctcttccatg ttttcttttc gaattagttc atattgtttt tgaattattt gattgttatt
    14641 cacttaactc acccctgctt ttcttaagcc atgcttcttc aagtgctttt gcgtcatatg
    14701 tacggttggc aaaattgttg aaagtcctct tttcgggctt tgaattcctg ccggcctttg
    14761 aagggttctt aaccaggtaa gcctccacct cgggcatggt ggaaaggccc ttcccgtgcc
    14821 attcggtcaa tatcctgtca atataactga aggatggctt cagggtgctt gcagtcagct
    14881 cgcaggcttt aagcactata tcctcgctgt attcaaaggt atagaaccac ttctgcagca
    14941 tttcctcttc ggccgaggtc ggctgcctgc caattcttaa aaagctgagc actttgttgt
    15001 acttctgcca tttttcctta taacgcgaat tatattgctt tgctttgtcc acagagtcta
    15061 ttccggcgtc aaaccaattt tttgccacct gcttgaggta tggcatctct ttcctatccc
    15121 ttgatacgca ttcctccacc agcattttaa ttacatcggg aggaaagtta tagtcatcca
    15181 tccaatcgag gaatacaaaa agctcatttg gtgacagctc tcttccgaac agtttacgga
    15241 aggaatcaaa catagccctt acatcctgat tgccccgggc atttattatg cgctcaggac
    15301 tatatttacc tgcactcggc ttttcttcct ttatattcag catgacttcc ttgatactga
    15361 gatattcaac cgtaaagccg ctttcagcat catccgggga gtagggatac agcttgatta
    15421 tatcttgctc cgaccagtac ctccaagcag catgcacctc atcccgggtg ataccgaggg
    15481 tctttgctac ggtatcggtg gaaagcatat tgtttacata actttgagaa tacttgagcc
    15541 ccaacaggta taccttgaca taatctgccg gtgcaaaggg catgtaatgg cttatgaaaa
    15601 ggttctcaac aatggtttgc ccaagatcct cgttatttga ttttattctg aacaagttca
    15661 taaataatgc actccttgga actatttaag cgtatatcat aattatacca catatgacat
    15721 atttatatgt cgtttatagt actgcttgta caaaaaaagt gaataaaaag ggcagctttg
    15781 aatactaata attacaagcg cttacacagt agatggtaaa gaatggacgt aaacataaaa
    15841 atttgaatat tctctgtttt tattgtagaa tagttctatg cagactatat gtcttgtgcc
    15901 agatacatat agctgacaaa accgtaatta ctgctgaagg aggcgaatag atggagggaa
    15961 gtaagttaaa ggaagcagca gcacggtact ggaacaagat ttctggcgga attgaaaata
    16021 ttaaaaagca tattaaaaat ataattaaaa agatagcaaa taacggagct aattcattaa
    16081 ataaacatac atattccaga aaaattgtga acaaaagaag gtttgccata gcctgcggat
    16141 tgattgcact cctggtattt gcctcgattt ttgtctatgc ttcagggaaa gcgtacaccg
    16201 tgaaggtgaa tggagttgaa atctgtaaag ttaaaaaccc aaagacggtc gaaagcgcat
    16261 taaatatttt gaagcagaaa taccaggaaa aggctgattc cgaaataagc ttcaccagcg
    16321 aagttacata tgaaaaaaca agagcatcaa gcaaggaaat acttaaggac gatgcattgg
    16381 ttgaagtatt gggcaagaat gttgaattca aggttcaggg gtgcagctta tatgcagacg
    16441 gtaatattat tgcaattctg aagacaaagg aaaaggccga ggagcttttg aaggagattc
    16501 aggacaggag cctggcggga gcagacaggt caaagctcaa ggaaattggt tttgcggaaa
    16561 aggtagagct tagggatgag tttatagatg cttcggaaat agctgataaa gacgagataa
    16621 tagattttat tataaagggg accaacgaaa tcaggatcca tgaggtaaag tccggcgaaa
    16681 gcttctggtc catatcacga aagtacaata tgtctcttct ggatttggaa aaggccaatc
    16741 cgggagttaa tcccgaaagg attcagatcg gccagaagat aaacctgatt gttcccaagc
    16801 cgcttatcag cgtaaagacg gtggaaacca tcacatttat tgagaaggcg gaatttgatc
    16861 agaaggtgga attcagcagc tcattatata aggatgagac tagtataagg gtcaaaggca
    16921 tatatggtga aaaagaagtt gttgccgatg ttacaaaggt taacggcatt gagaccggaa
    16981 ggacgattct cagtgagaaa atgctcaaag agcccaaaac acagataatc gttaaaggga
    17041 ccaaagagcc gccgcccaag aagggaacag gaacgttctc atatccggcc aggggaagca
    17101 taacatcaag atatggaatg cgatggggca gaaggcatac aggtatagat atcggagcac
    17161 cttatggttc tgatgtaaaa gcttcggatg gaggagaagt gacctttgtc ggttatcaag
    17221 gcggatacgg caagcttata ataatagatc acggtgccaa ttttgtatca tactacggac
    17281 atctcagcaa gtacagtgta aaggtcggac agaaggttta caagggccag aagataggcg
    17341 aggttggcag ctcgggaaac agcacggggc ctcatgtaca ttttgagata cgcaagaacg
    17401 gcgtcataca gaatccggca aactatttaa agtaatatga agtatatata aaaagattga
    17461 gggattaaaa ttaaaatttg aattccccaa tcttttttat ttcaataata ccggctccct
    17521 gagcggtctt aagctcattc agtgagatgc agaatctctt aagcaggctt ttaatatctg
    17581 aaggcggcag gttaggcttt ttctgataca gaagcactgc ggcagcggca gtcatgcttg
    17641 ccgcagcaga ggtcccgctt agttgagtgt attgctgccc tgaaagcttt gactggtatg
    17701 acgatttact tcccggtatg taatcggtat cggcattaag ggttgctatc ttatatccgg
    17761 gcataactgc atcaggtttg tcaaacttgc catccacagg accgcggccg ctgaagggtg
    17821 caactcttga agaagatccg gaaacagtac atgctcctgt tgtgaaggcg gaaccgcaaa
    17881 ttccgggaga agttatactg gcctcatgag ggcccaggtt tccggcacag gtgcagataa
    17941 ataggccgtt gttccaaagg gcctccgatg ccaggctcag gatatcgaaa tgcctgcttg
    18001 tgcagcagca gccaaaggga agcacaagga ccttgatgtt gtatttttcc tttatactgt
    18061 atatccactg cattgcagcc aatatatccg agaagcatcc atagcccaag ctgttgaagg
    18121 cctttgcaca tacaatattg cagtcataag ctgctgcctt gaactttccg tcaatagagg
    18181 ctccaaaggc cgctcctata caggcggtgc catggccgtt atcatcataa ggctcattga
    18241 tttcattaat ataatcctta aaggctgtga ttctgttccg gggtcttgtc aggtccgggt
    18301 ggggatacac gccggaatca ataaaggcgg cagttatgcc ttttccgctc aaatgaacag
    18361 aggctgcttc ggggctttca agggccttat ccccgctttc agtgccgccc ataagttttg
    18421 cagtaccgtc aaaatatatt tttgcaattt cgacaaaact actgacagaa tcaaaattac
    18481 cgatacttat tttaccggtg actgcattta gaaagggaat atggtatttt atcctgagac
    18541 ccttccgctt cagtgccgat tcggttttcg gatcaagagg ccttttgaaa gttataataa
    18601 caggtatagt tttaaccttt ttgatggaaa tatatctttt tactgccgga tcaacctttt
    18661 taaaactttt taaacataag gaaattgaaa tcatggttat cccccggtat tattgattta
    18721 tcaatagcaa aagcctaatt tcataatatg ctgttgaaaa aaattggtga atggtatata
    18781 aatatttata gtaaaggagt gttgctcatg gctttaatag attttaaatg catcaagtgc
    18841 ggtgaaaaat ttgaagagat tgtaaagcta tcggacagag agaacataaa gtgtccaaaa
    18901 tgcggaagcg aggatataaa acaggttttt gaaggaaaat gctgcagtgt gggatcatca
    18961 aaaggggttg gaggctgcag cagcggaagc tgcggaggct gcagcggctg ccattagagt
    19021 atcagggtat caaaaaatac caatgaagtt aacataaaat tttgatataa tatatttgat
    19081 tcactgattt tcaggagtta ttgctcttgt tagaagggat taagtatatg aaaaaaagaa
    19141 tatcaaaacc ggcaatagta ctaatattag cagcagtact attttttttc accggcgata
    19201 aaggctttat ggctgtaaaa atgcagggat atgaagccct caaagtattt gctcatataa
    19261 aaagcagtga attcacccct gagttcaata tatatgagac ggagcatttc atcattaagt
    19321 ataccgataa agatgaggat acagtaagag acatagcggg aatttttgaa aagagctata
    19381 aagtagaagg aatacactac ggtttttatt caaaggataa aaccatagtg tttgtgtaca
    19441 atgatcagga aagcatgtgg gattatcagc agtctgtcaa aggacaagca gttatggggc
    19501 tttacaatct gggtattatt cacgtgctgt cacccaaagc atatctggac agcatgcaaa
    19561 tatatgccaa ggattatgaa gcggacgggc cgatacttca tgagtacgta cacagggtga
    19621 ttgactatag aagcggaggg aacgtagagc tgtggcttac ggaaggactt gccctttatg
    19681 aggagtatga tgtggatggg acagagtggg caaaggggtt tagctatgaa aggcactaca
    19741 ctgcccatga gcttagggat gactttatgg aactggatga aacacagtcc tacaggcagt
    19801 ccttcgatat tgtatggagt cttattgagc agaggggaag gaatagcata aacctcatgc
    19861 tggacgaact taaatatgga agcaccctgg atgaggcttt tattagaata tacggagaag
    19921 atctgaatga atatattgat tcaggtgcct ggaagaggta gaaaaaaaag agcggtcgaa
    19981 aagaccgcct tctttatatc cggggggata taaaacatat tgttgagatg tttacatttt
    20041 atattactct ttccaaaatt agtataaggt acaggcctta ttttaatagg acaatggtcc
    20101 ttaattgatg ttagtactaa cgtccttttg tactgtatcc gccggaaaaa cctgacttgt
    20161 tgcgtaatag gagcataagt agtaaaatat gtgtaagaag aatgcgactt tgtcgcattc
    20221 ttcggacacg agatattcag ggtattcctt atgggtatta tggaacgggt atcacggaac
    20281 acacggattt aacacggagg acacggctga ttttattttt cccgcagtga attctgtgga
    20341 tttcggtgtt ttcagtgtta tgcgttacct gtgtcacatt tttctatata ttaatggggt
    20401 ggtgtatttt gatggaaaac agaagaattc ttgtggtaga tgatgaaagg ccaatttcag
    20461 atatcataaa attcaacctg gagaaggaag ggtacaaggt atttgtggcc gaagacggac
    20521 aacaggctgt gaacatgacc tacgagcata aaccggatct tattatactt gatattatgc
    20581 ttcctataat ggatggcttt actgtgtgca agaagctgag ggagaatgtc agcactccga
    20641 taataatgct tacggcaaag gaagaggaag tggacaaggt tctgggcctg gagctgggtg
    20701 cggatgacta tatgacaaag cccttcagcg tgcgggagct tatggccaga gtcaaggcca
    20761 acataagaag ggccaatttc cttaattccg gtttggaagg cagcgggcag gttgtaaaat
    20821 caggcaatct gataattgat ttgaacaaat acgaggtccg caaggatgat accgtactgg
    20881 agcttactct aagagaattt gagcttttga gatatctggc acttcatgcc gaacaggtct
    20941 tttcaaggga aacgcttctg gaagaggtct ggggttatga atactacggg gatgtaagga
    21001 ctgttgatgt tacggtaaga agactgagag agaagattga ggatgacccg agcaatgccg
    21061 tgtatgtgca gacaaagagg ggcgtaggat attacttcag gaggatttaa tagatgttta
    21121 aaagcatcca atggaagctt gtaattatat ccttcctgtt ggtatggctt gcaatgtcca
    21181 tagtaggcgt attcatcacc gaagctataa aaaaggaaca gattgacagc atgacagata
    21241 ctattatcac aaggggctat tatctggctg acaatctgaa ggacaaaata gggggagcgt
    21301 ctaatattca ggagattgtg tcaaattggt ttgtaggcca gggtaagcag gtaagggctg
    21361 tttttgtgta tgaaaacggg ggctttacag ccagccagac caattatgaa ggagctgtgg
    21421 atgatcttga cctcctgggg ctctggatag tggaggaagc tatagaccaa gcacaggaaa
    21481 tcacatggcc cgttgataac tcaaaatatc tcatgagtat tgccgtgcct attatttcct
    21541 ctaataatga tgtagccggg gcaatatata tcagtgcaga tttattcggt attgaagaaa
    21601 acataatgag catcaataaa atacttacca gtgcgactat tttggcacta tgcataactg
    21661 cgcttctggg caatctgctg tcaaggactt ttacaggtcc catcaaagaa gtaacttcaa
    21721 aggctgagag actggcaaag ggggattttg ggcagacaat agccgtaaaa tccagagatg
    21781 aggtaggcca gctgaccgaa atgttcaact atcttacggt gaggctgaag agcacattgg
    21841 atgagatgtc cagggaaaag aacaaggttg aagcaattct tacaaacatg accgacggaa
    21901 taatagctgt aaatgccgaa ggggtagtaa tacacgcaaa tcctgccgct tataagattt
    21961 tcaatatccg ggaggaagac ctttataaca ggaatttcga cgatgccgca gagaagctgg
    22021 atcttgggat aaccttcaac gatattctta atgatagtga taataattac aatattttaa
    22081 gcataaacaa cctcataata aaaatcagtg tagtgcagat aataaatgaa cataataagg
    22141 ctgaaggcgc catgctggtg cttcaggatg taaccgaaca ggaaaagctg gataaaatgc
    22201 gcaaggaatt tgtggccaat gtttctcacg agctgcgaac accgcttact acaatcagga
    22261 gctataccga aaccctactt gacggagcat tggataacaa ggaatatacc ttgaattttc
    22321 taaaggttat aaacagtgag tcagaaagga tgaccaggct ggtaaaggat ttgctgcagc
    22381 tttccaagct tgattatgac aagatggaat ggaacatgaa aagtcttagt atactgaata
    22441 tattaagaga ttgtgtcgta aagatggaga tggccgccaa gcaaaaaaat cagtccttaa
    22501 gctttgaagc tattggagag ctgtgtgaaa taaacgggga taaggataga atagagcagg
    22561 taataataaa tataatcagc aacgcaataa aatatactcc ggaaaacggc agcatcaaag
    22621 tgacggcaaa aagactgaag gatagcgtag agataaggat agccgacagc gggataggga
    22681 taccaaagga cgatctgcca aggctgtttg aaaggttcta cagggttgat aaagcgcggt
    22741 caagatccat ggggggtacg gggctgggcc tttcaattgc caagaacatt gtggaagccc
    22801 ataaaggctc aataagaata gaaagcgaat atggaaaagg cagtgaggtt ataattaatt
    22861 tcccttgtga ggaaagttta ttgtaaaata agtttaatta gtctgtaata atgctgtaat
    22921 aaaattctta tataataata ctaaggatta gtatgtatta atatggtaat tagttaggtt
    22981 aatatgtccg cgggaggaat tctcatgttc aggaaggtgc tgattacagc aatcattgtt
    23041 acagcggtat ttgcagcagg ctcggcagtg tatgcccaat cagggacaga ctatgcagaa
    23101 gaaactgccc cggtatttaa gctgctttca gattatgaat caaaaacatt tgacagggat
    23161 tacatgatta ccggcagtgc aagtgaaggt actgtagtta ctatagaact gtattggttt
    23221 aagagcgaaa agagcattat agtcaataag aaacagtccg cgtcctcgga aagtgaagga
    23281 gattggattt ttcaggattc aaaagagtac acagtcggat cctcagggat tttcgcagaa
    23341 cctgtggccc ttaatctcgg gaaaaataaa attgtgcttt tgattagtaa tgcagacggg
    23401 gaaactgaag aaataacatt ggaattggtg cgcagtcttg agaaggatgc aaaagaggaa
    23461 gtcaatggtc cttccttgaa taagttcgtt gaggatttat caagtaaagt gaactcgact
    23521 gaataagctc atatgggtga ttatattgaa ccgcgaacgg attaaatcag tatctctttt
    23581 atttcttgta atattaagtt ttgtattgac tacccgaata tggttttata cttccattga
    23641 gggtgttttt attatgtccg gaaacaaatc aataaagccg gattataatg cggaatatga
    23701 caaatcggat cttctcaggc ctcataagct tatagtcagc tttggagaaa gaagtacgct
    23761 tctatatgac aatgaagaat acagtgaagc atacggcagg atattcaaag aagccaagag
    23821 ggtaatgcgt actgcaatat tggtgaacag caaggaagtt gtgaaaaaaa ttcccatgga
    23881 ggaactgtac aaaatcagaa atgcaagggg aataaccttc atctataatt tgcctcttga
    23941 gatggaagca gtatataagc ttatgaatat aaacagctat ccggatacag gcatcaggag
    24001 cattgatgag ataatcgttg ctcagagcct ggacagggca tatctgtgtg attccgtcca
    24061 gggaattctt tatgagttca ggacagaagg aatacagaat aatttggatt atctgatttc
    24121 gaatctggaa aagcagccta cggtgttaag cctttatctt gacagtattc aaccggagat
    24181 gtatggcaaa aactctgcag tgccggcgag aatcggcgca aatgggtttc cggttttgtc
    24241 gggacaaaag gaggtggaag ggtcggatga gatacctgaa agcatagcgg gcttgttcga
    24301 tgatgaaata acttccctca gcataattaa gaatatggac ggtactattg tgtacaccaa
    24361 cagagagggc caggtggtta aactgtatac tgacggcatg ctggaatatg tgaattttaa
    24421 tctgcagccg gaggacaaaa ccacggtcgg tttgggcggt gccattgata taagcactga
    24481 atttgtaagc aggcattttg gctttcccga ggattgctat atatctgata taattaggat
    24541 aaacagtatg ggggatgaca agtatataat cagatacaga tatgcctatg agggattgcc
    24601 tgtactgtca gcctccgata taagcagcgg ctctattgaa gttgagatcg taggggatga
    24661 agtcagaaga tataaaagga tcagacggac attgaaccat aactttgagt ataagcaggc
    24721 catgaattcc ataaatgtgc ttgatatact gcttgacaaa aaagtagagg ccttaagcgg
    24781 tgaaagaata aagaaagtca acgatatgta cctggcctat tatgaaagat tcggtcaaaa
    24841 cagcataaca tatgtgcctg tttgggttgc cgaagttacg gtagaaagga ttgacaaggg
    24901 tacggtactg aaccagaaat atataataaa tgcagagaca gggatcatac tggataaata
    24961 ggatccgata taccttaata cggaactgtc agtgcaggac atacaggaga gggtttttat
    25021 ggattgggca aaagcaaaga caattcttat agttgttttt acagcgatca atatctttct
    25081 tggatatatg gtttttgcag ccggcacagg tggttttgga tatgttgaca gtgagaaggc
    25141 aaagcaaata actgactatc ttgcagaaaa ggatgtaact gtaacaggca gaattccggg
    25201 gaaaaaagcc gatatgccgt ctattatagt taaatataag cttttcagca aagaagatgt
    25261 aaaaggaagc tttttctcca caggggatgt gccggaagaa tcagcctcag aaaacacagt
    25321 ggggttaaaa ggaaagaata ttgaaataac aattagaaac aaccgggagt tttactattc
    25381 agacaacggc ataaagccgg ccggggcgac agatgagaaa gcttgcagaa agaatattag
    25441 ggaatttttg agcaggctta atataactga taatcttgat accggaatat cggaggatat
    25501 tgaaggctat aagaggtttc ttttttcgca gtcattcaaa ggagcggaga tttacaacag
    25561 tattatggag ttttatgtaa atgattccgg agtgcatagg gcaaggatag tatggtttga
    25621 tacaataagg caggcaggga agaagacaga agtgatttct ccgttgatag cgctgctgta
    25681 tgtgccggaa cataataaga aaagtgagct gcccggcagg gaagtggtgg aaatccgtca
    25741 gggatattat tttggaaccg gtgcaggaga acaggttgat gtttctgaag tagtggaagg
    25801 aacggctttc cctgtatgga agattgagac tgatagggat attatctata taaatgcata
    25861 taatgaaaaa gtggaaggaa ttgagaaaag gcaataaaat tgcctttttc taatatattt
    25921 aataaaatat aaatcaatga tataataaaa atggcatatt ttcattcatt aacatacaaa
    25981 ggagcatata cttttggaag aaaaacttat aattaaaggg aataaaaaac tacaggggga
    26041 agtgtgtatc agcggtgcaa aaaactctgc ggtagggata atacctgcag cgctgctgtc
    26101 cgattccggg tgtattatta aaaatttacc ggatatagag gatgtgagat atcttaagaa
    26161 gcttttgcag caagtcggcg caaaggtgaa tgatggaaat accggggaac tggaagtcat
    26221 cagcaagggc atgaaaaact acagggtgaa ttttgaaata gcaaagaaaa tgagggcttc
    26281 ctattacttt ttgggagcgt ttctgggcaa gttcaagaaa gccgaagtac cttttcccgg
    26341 aggctgtgat ataggaataa ggcctattga tcaacacata aaaggccttg aagccctggg
    26401 ggcgaaaatc aaggttgagc atggtattat taaggcaaaa gcatcggaat tggttggcgc
    26461 acctatatac ctggacgtgg tgagcgtagg tgctaccata aacataatgc tggctgcatg
    26521 caaggcaaaa ggagtaacca caatagaaaa tgcggcaaag gagccataca tagtggatat
    26581 tgccaaccta ttgaatgcaa tgggcgccaa tataaaggga gcagggaccg atgtcataaa
    26641 gataaaggga gtagagcatc ttggaagctg tgagcatatg ataatacccg atatgatgga
    26701 agcgggtact tacatgattg cagcagccgc tacccagggg gatttgcttg taaagaatgt
    26761 catcccgaca catcttgaat cggtatctgc aaagctaagg gaaatgggca ttgaagtaga
    26821 cgagaacgga gactccataa gggtcacgac tgtggcaagg ccaaaggcag ttaatattaa
    26881 gacaataccg tatcccggct ttcctaccga tctgcagcag cccatgagcg tgctgctttc
    26941 catatccgag ggcacaggaa tcatcaccga gagcatatgg gaaggcaggt tcaagcatgt
    27001 ggatgagctg aagcggatgg gtgccaacat caaggtagaa ggacgcgttg caattattga
    27061 aggtataaac aagctttcgg gatctccggt acatgccacg gatctgagag cgggagcggc
    27121 aatggtaata gcaggattga ctgctgaagg ggagacggaa atagataatc tgaagcatat
    27181 tgacaggggc tacgaaaaca tggtggagaa attaagaaat gcaggagctg acattacaag
    27241 ggtgcaatgc taacagttgt aatcggcaat ggaggaagag atggggataa gactttgctc
    27301 cctttcaagc gggagcagcg ggaattgtat ttttgtgggg aatgaccgca gcggattgct
    27361 ggtggactgc ggcataagcg gcaaggaaac actgaaaaac ttacatagca taggtgtttg
    27421 ttcaagtaca ataaaagcgg tcgtagttac tcatgagcat tcagatcata tcaagagtgt
    27481 cggtatcata tcaaggaagc ttggaatacc gatatatgcc aatattaata cctggaacag
    27541 catgagagct ttgataggaa cggtcaagcc ggaaaacata agatctttca agacaggaga
    27601 agaactggag attgcaggaa taggaatcaa gtcattcaat attccccatg atgcggctga
    27661 tcctgtaggc ttcagcttct atgaagggaa taataaggtt agtatagcta cagaccttgg
    27721 atatttcagc gatacagtaa gggagaatat cagaggctct gacatgattt tgctggaatc
    27781 caaccatgac attgagatgc ttatgacggg aagatatcca ttttttttaa agagaaggat
    27841 aatgagcgag cgggggcacc tttccaacga agcggcggga aatgccgtaa atgagcttct
    27901 gcagacggga gtgcgtgaag tgcttcttgg acacctgagc caagaaaaca acttccctga
    27961 gcttgcctat gaaacagtaa aggggatatt gaaacaaaac aatataaatg ttgatgcgga
    28021 tattaagctg gatcttgctc caaggaacgg gataagcagg ttttataata ttgattgaaa
    28081 ttttatgccc atcagatgga tatattaata tcagagtatt tacccggata aaataaattt
    28141 ggctgtggag gatgaaatta tggataattt cgatgaaaat tcatatccgg aaattcaggg
    28201 caataaaact gcagaagaga ataatacttc agctccgact gcttatagga atgagcccat
    28261 aagatataaa aggccctcgg gatttttatt tctgaccact gcgctggtag gcgccgtaat
    28321 aggaggactc attgttgctg caatagtgcc gtcatatttg tatgacagaa tgctgtctgt
    28381 gcagcagggg ggcattgcac cttcaggaca gcagttggtg atacagccat ccaacgagat
    28441 gaatgtagct gcggcagtgg cacagaaagt aacccccgca gtagtcagga taagtacggt
    28501 gacagtggag agggacttgt ttttcggcaa gaggtcttat gaaggagtag ggtcgggtgt
    28561 aataattgac ccaaagggat acatacttac taacgcccat gtggtgggtg agcatcctga
    28621 ggaactgaca gtgtttttta aggacggaaa agaactggac ggaacggtgc tatggcagga
    28681 cagcctcctg gatctggcag tggtaaaggt tgaggcaacc ggtttacctt cagcagagct
    28741 tggggactct gacagcctta ttgtagggga aatcagcata gcaataggaa atcctctggg
    28801 tatgcgtttt gaaaggacag ttacccaggg tatagtgtcc gggctcaaca ggagcattat
    28861 ggtggaacag ggctctgtaa tggaggacct tatacagacc gatgccgcca ttaacccggg
    28921 gaacagcggc ggccccttga ttaacggcag gggacaggta ataggcataa atacggtaaa
    28981 ggcatctgca gaaggcctgg gttttgccat tccgataaat attacaaagc cgataatccg
    29041 aaagcttata gataccggtg agttcatacc tacatatatg ggtattactc ccctggacag
    29101 cgagatgctg ggctattatg caataaatat agagatacaa aagggaatat acatatacaa
    29161 tattatgaag ggtgcccctg cagcatccgc aggactggag gcaggagatg tgataactca
    29221 tatcaacggc actgaggtaa acacacttgt aaagttcaag agcctgcttt acagcttgaa
    29281 tcccggggat accgtaagtg taaggtactt aaggagcaat accgaataca attcagatat
    29341 aaagctggca gaaatgccgg aggaatacag gtaatgaata tatcgataat cacagtcgga
    29401 aaactcaaag aaagatattt aaaagaagcc gtggaggaat actcaacacg gctttctaaa
    29461 tattgcaagc tggatattat ggaagtaccg gacgaaaagg ctcccgagaa cttaagccct
    29521 gcccaggagc tgacagtcat gcaaaaagag ggacaaggca tactccggca cataaaggag
    29581 gatacctatg taattgccct tgccatacag ggaaggcaga tatcctcaga ggggctggcc
    29641 gacttcatca acgacctggg cataaagggc aggagcaata tagcttttat aataggcagt
    29701 tctctgggcc tcagtgaaga agtgatgaag cgggctgact tcaagctctc cttctccccg
    29761 atgacctttc ctcaccaact tatgcgggtg atgctgctgg agcaggtata cagggggttc
    29821 aggattatca agggggagcc gtatcataaa taaaggcaaa aagtacataa actcaatcag
    29881 gctataattg aatactcatt ttgtaagact tatttcataa gataattaga ctaagtttta
    29941 taaaatagat taacacctcg aatgggaggt gtttttttat agaaaaagac ttgaaatagt
    30001 tagcataacg gattaatata ttgaatatag atttaatgaa ctaaaattta atgattagga
    30061 gactttgtaa attggttaca agaaaagaac aaaaatataa acgaaggaat gaaatccttg
    30121 aagcagggct agatcttttt atccgcagag gttatgcttc cacaaagata agtgatattg
    30181 caaatcaggt tggaatgagc acaggattat tatttcacta tttcgaatca aaggagaagc
    30241 tgtatgtgga actcattaag aatggattag ctggccctat gagtattatg acagattcgg
    30301 gtatggagcc cattgttttt tttaagacta ctgcagattg gattttgggt tctctaaaaa
    30361 ctgatacggt ttttgcaaag tattttgtac ttatgagtca agcatattac aatgaatcag
    30421 cacctcaaag tgtgaaagat atgctaaagg gatttgatat ttatactcca acttcacatt
    30481 tgataaaaaa gggccaagca aatggctcaa tcaaagaagg tgaaccctat gcacttgcca
    30541 tagcatattg gtgcgctata cagggaattg ctgagcaaat ggcaatgacc cctgaatacc
    30601 catgccctga gggtcaatgg atagttgata tattaaggag gtaaagtatg aagaagataa
    30661 ttattgttgg tgcaggtatt gctggactat cagcaggcat ttatgcaagg cagagcggaa
    30721 tggatgttac catttttgaa agtcatacaa taccaggtgg agcctccaca agctggagaa
    30781 gaaagggata cctttttgag ggaggaatgc actggttgac gggatcctct ccaaaagtgc
    30841 cgcttaataa ggtttggaga gaacttgggg cgctgaacga tgatgtccca gtttataata
    30901 aggacccttt cctgactgtg gaatttgagg gtaggacagc atttctttac cgtgatgttg
    30961 aaaagttaag gcaacatttg ctggagcttg cgcctgaaga tagaaatgaa atagacaagt
    31021 tatgcaggga tataaaaagg ttctccaagg taagtatgcc tattagggat ataaagaacg
    31081 taaaagtgaa aaagaagact tcagtaccat tatcagattt cattaaaatg ctgcctgcat
    31141 tctcaagaat gtctttttat gcaaatcaaa ctgccggaga gtttgcaatg aggtttaaaa
    31201 gtcccattct cagggctttg attaggtgta tcattggaga ggagtattcc gcaattggtt
    31261 tgatatttac aatcgcaact cttgcttcgg gtgatggagg ctatccagag ggtggatcac
    31321 tggctatggc tagccggatg gcaaagtatt tcgaatcatt aggtggtaaa ataaattaca
    31381 agagcagggt aacacaggtt tctgtaaaag acggaatcag cgatggagtc attataaatg
    31441 gggaacactt tcctgctgat gcaataatag ttacacagga taccctagtg gcaattgaca
    31501 gcctttttga tcctccaata aatgaaccat gggcttacaa gatgcgtcaa agcataaagc
    31561 caacgctgaa tacatttata tgcttaggta tagaggcaga tttgtcagat ctacctgaaa
    31621 caattagctt catgcctcat aaaccgcttt tatgtggtgg agctgaagta gaatttattt
    31681 ctatcaataa ctattctggc tacgaaggat atgcaccgaa tggttgcacc gcagttacct
    31741 caataatttc tggggatacc tatgattatt ggaaggcatg cagggaacat ggcacctatg
    31801 aagaagaaaa gaggaagctg gctgagaatt ttattgaaat actggctgaa aggttcccgg
    31861 aaatcagaga aaaggtggct ttgtgggatg ttgcaacacc actgacatat gaaaggtatc
    31921 tgagttccta taaaggctca tggatgtcaa taacgggcaa gggagaaaag atgaatggtt
    31981 atccatcaaa gtccgaaaaa atacaaaatg tatattttgc aggtcagcgg ctttctcctc
    32041 caggtggttt gccagtagct gttgaaaccg gaaggaaggc tgtacaatat ctttgcaggg
    32101 atacgggaac aatttttcaa gcgaatataa atgacggtat ggtgcccggc acttattagg
    32161 agaaggaaag actacacagg cggctatgat aaagatggaa atcttacgga cgaccccaat
    32221 gccatatttg atggaggaga ggttcttcct tcaggttatt ggaggggatc tggattttcc
    32281 atagtcattg acatggttgt ttcagcacta tcagttggca aatctaccta tgatatgggg
    32341 atgaacaaaa acgataaagg cgtttcacaa gtgtttattg caataaatcc catagcatat
    32401 gcaggtgaga accatgctga tgcttacata gaaaatatgt ctcagtatat taagacatca
    32461 gcaaagaagg caggtgttac tgatatcctt tacccgggag aaaaggctgc cagagaaagg
    32521 atagaaaata tgagagaaaa tatccccgta aacaaggaaa tgtgggaaac attactgaag
    32581 ttataactag cgccttttaa gtgacaatgg gacaggcaca ttgttccaag atatatcaat
    32641 aataccaggc tctcatgtat cagagcataa aggagagaac tatgaataac gctgatttct
    32701 acaacacgat atttaagaga aagtccatac gcaaatatag aaaagaaccg ctggaaagct
    32761 ccaggcttat ggaattgcag aagtttatgg aaaggcttgc gcctttatat gaggatatca
    32821 aaacagaaac agccatactg tcggagaagg atattaagtt actgctgcct ataagatcac
    32881 cgcattatat tgcgatatat tctgaaacaa agaatggata tttgcaaaat gcaggcttta
    32941 tgctgcagca gttggatctg tatctgtctg caaaggaaat gggggtttgc tggctgggcc
    33001 tgggcctgcc tcagaaggag tctgcttcaa ggaatggcct ggagtttgta atagctatgg
    33061 catttggata tgctgatgag ccccttcaca ggcaaagcac aacagagttc aagcgcaagt
    33121 cattgcctga aatatcttct gtaaacggtg ctgaggaact tcttgaagcc gtcaggctgg
    33181 cgccatctgc ttcaaacaca cagccgtggt atttttcagg cagtacggat tgcataataa
    33241 taagccgcag gctgcctaat atattgaagg aagcactgta cagcaagttc aacagaattg
    33301 atattggcat tgcaatgtat catctgtggg ttgcggcatt gcatcagggg aaatccatag
    33361 agtttaccag ggataattat acagtgccaa aaggaaatga atatatggcc acggcccata
    33421 tcacaggcac tgacaaaaaa cttcagactg ccatatttgc aggcgggtgt ttctggtgca
    33481 tggtttctcc ctttgatgtt ttagagggaa tcattgaagt aaaatcggga tattcgggag
    33541 gacacaagga aaaccccaca tataaggatg tgaaatcaca aacctccgga cactatgaag
    33601 ttatcaagat tatttatgac cctgagataa ttggttatga taagcttctt caggcatatt
    33661 ggagacaagt tgaccctaca gacgacgggg ggcagtttca ggatcggggt gaatcctata
    33721 gaacagctat tttctataca aatgaagagc agaggcaaat ggctgaagca tcaaggaaag
    33781 ccatggatga gtccggacgg ttcccgggcc ccatagttac tccaataatt ccggctgctg
    33841 tgttttatga agcggaagaa taccaccagg atttttataa aaaaagccca aaggaatata
    33901 aggaggacag aaagatttcc ggcagagatg aatttataaa gaagtgttgg ggcgatgaat
    33961 actatgatat ttataaataa agcccggcaa atatgatgcc tggcacttat gcttttacct
    34021 ccaaatcgtg tataatcagg tatgaatggt agttcggaat ctggtttttt gggaggaata
    34081 tgatgaataa actggagatt atggggagag acatcaggaa ggatctgcag ttatttgctc
    34141 aggagctggt ccgtatcaaa agtacgtcgg ggaatgagga aacgataatt agatttatag
    34201 caagtaaaat ggaagcctta ggctatgatg aagttataat cgatggattc ggcaatgtgg
    34261 ttggccgtgt tggaagcggt gaaaaggcag tcatgttcga ttcccatgtg gacacagtgg
    34321 aggtaaaggg tgcggagctg tggaaggtgc ctccattcag cggcgaaatc atagatggct
    34381 ggctctgggg aagaggctca gtggatatga aatccggcgc agcggcgtcc atatatgcgg
    34441 cggcaattgc caaaagccag ggactgctgg aaggaaaacg ggtttatgtt tcctgtacta
    34501 tttttgaaga ggactgtgat ggggagaact tgaagcatat gtttaaagaa cttcagttca
    34561 agccggattt ctttgtaact tgtgaaccgt cgggaaatat aataactacc ggccacaaag
    34621 ggaaagcaca gattgtaata aagacacagg ggatttctgc tcatggctcg gcaccggaaa
    34681 aaggagtgaa tgccatttat gaaatggcgg aaatcataaa cagagtagaa aaaacaaacc
    34741 tggagcttat gactaaggca gctccgaaag gaacaatggt gatgtcaaga atctccagta
    34801 ccggtgcatc gctcaatgca gtgccttctg agtgtgaggt ttatttggac cggcgtatgg
    34861 ttgtcggtga aacagaggaa ataatcaaga atgaaattga aaagatcata aagggaaaga
    34921 atgccacttg ggaaatagga actttgcata ggaaaacatg gaccggaatg gacataactt
    34981 acgagccctt tcatttggca tgggaaatct ccttggatca caaacttaca aaagcattta
    35041 ttgattccta caaggaggtt tttggtactg aacctgagaa atatgatttc tgggacttta
    35101 gtacaaatgc tgttgcgaca gttagtatgg gcattcctac cataggcttc ggccccgggg
    35161 aatacaagct ggctcacatg acaaacgagc gctgtgaaat aagccagatt atcgattcct
    35221 gcagcatcta tacaaatgtg ataactaaat tgtaactatg ttttccggca cttattcaaa
    35281 gcatgagggg gaataacgtg agtgtaagca tgtttcatag gttagaaggc aggaatatat
    35341 attttaaacc actgaatata attgatgccg aagcgataca cagctatgcc tcggatgaga
    35401 atgtcaaacg atttattggc tggccattga tgaaatcatt ggaggaaacc cgcagctaca
    35461 ttgaagacat gctgaagcgt gagtcggcag gaacacattt atatgcatcc gtagctctta
    35521 agtcaacccg ggaaatcata ggcacggtga tgatttttaa ttttgaccgg gaagccaatc
    35581 atgcggaaat cggttatgtt tttcacagca aacattgggg taaggggcat ggtacggaaa
    35641 gtgttgcttt aatgtgcagc tttgcatttg aaatacttgc tcttcacaag atccatgccc
    35701 gtgtggtcga tgctaatatt agttctgccc ggatattgga aaagaacgga tttctgctgg
    35761 aagggcggtt aaaagatcat tatttcatag acggaaggta ttatgacagt ctgatttacg
    35821 gaaagtttga agctgatgaa aaaacattgt catactaagt aaaggagctg gattaagttg
    35881 gctaaagtag ttattcaaga atgcagggaa tatgaattaa acggtttgat gataaaaatt
    35941 aacgacggga tagaaagatt gggaggatgg gattcatttg tacacccggg gataaggttc
    36001 tactgaaggt aaatctcata ggctcaaaat catctgattc agcggctgta actcattctg
    36061 aatttgtccg ggcagtggca aggattttaa agcagcgagg atgcgaagtg tggatcggag
    36121 atagttcggg gggagccatt ggcggcatag cacctactgc aaagagcttt aggacagcag
    36181 gctacgagaa agcggcaatg gaagaagggg ctgtcaaaaa tgtattcgga tgcattcccg
    36241 gcctgcgaaa agcaagatat cataaaatgg ctcccaatcc ggagagcttt ggtgaaatcc
    36301 tggcggatat tcatcagaat gcagaattta aactgccaaa gcgctataaa agcggtaaaa
    36361 gcgaaacaat aaagtactga ttaaggttat tgatttcttt aaaaccgcac aaaaggtcaa
    36421 caggaaaaag tgcaggaatt gtaatgtatg tgtggagagc tgccctgtaa aagcgattaa
    36481 cagggatact aagcaaattg attataaggt ttgtattgaa tgtatgtgct gtcatgaatt
    36541 atgtatgtat aaagcagtag agctaaagaa ggaaaacttc cttgccggca tattaatcgg
    36601 ccttaaccgc ggtaaataca gataaaaaaa caggcagtcc aattgacgtg cctgaattca
    36661 catattagca cttacaattc tttaggttcc agattcccga agtattccca aataccattg
    36721 ttatatataa atataatata atcgttaata gcaagagaga agtcgtccat ttttatttca
    36781 cctgcaattt caaagattac agctttcaat acaccatctt gcttccctat gtaaagtata
    36841 ttttccttgg aaatattgtg ggtgattttc agacctacaa gggcctggcc ttttattaat
    36901 ctgcctgatt ttataaaatc ggttatgtgg gatgaattac tactaatgtg gcattttttg
    36961 gcacttgccg aattatatat gttaccatta taaaaattgt ttaaaaaatc tcgttcagct
    37021 tttagtgctt ttgcatcttc ttttgcattc ccggatgtct tcatattaag aaaatgtatt
    37081 tctgcaagtg cgtcgctctc cagtatgtct gagttttcga atatatcgtg aatttcttct
    37141 tcagcatcat agattatctc tataaaggct tcgtcttgca acagattttt gaaggcttct
    37201 tcagatggtg ctctttttaa atataattta ttcagggaaa cagctttttt taaatatgga
    37261 cagtagtctt tggtatttcc cgttttaacc aacgataagc tcatatagta atttgcaacg
    37321 gcatcgtcag ggagtttctc caaccccagt tcaagtgcct ttatggcttc gtcgtatttg
    37381 gataaatggt atagggaaca ccctttgtat atgtagacaa atttatcgga ggtattgtaa
    37441 tagatagcta aatcataata ctcgatggct ttttcataca attcttgaag ataataagta
    37501 tcgcctaagg cttcaatgat atcagcatca aatggattga gctttaaagc tttgtcatag
    37561 cattctatgg cttcggaata tctttcgagg gagaataagg aattcccttt actttgatag
    37621 gcatctccat aatccgggtc aatttttatt gcttcatcat acatttttat tgcttcatca
    37681 tatttgaaga gaaaatataa catcacaccc ttgctgtaat aagcatatgc gttggcaggc
    37741 tccaattcta tcacggcatc atagtttgca atagcttctt catattttct ctggtaaaaa
    37801 caggcatcac tcatgtccaa gtacgcctgt atatatgtag gatccagttc aattgccttt
    37861 ttataattat tgatagcttc tgaataattt gactgctcga agaaggcaag tcccttatta
    37921 tagtagtctt cggcggagag caattcttct gcaaatatgt ctaatgaaaa caaaaacatc
    37981 atcattatga taagcggtag aatgaaacga gcttttttca tatccaaatc tcccttatgt
    38041 tatttatacc aggtaatatc aggtttattg tttttatagc cgtatttcac cttttttttg
    38101 tatattaccc atctttttgt atctgcattg taataggctt cctggttggt atagccttgg
    38161 ggccgtgtag cgaacgacca ataaagcgcc acatggacat aaacattctt acgaggatta
    38221 atggctttta cctttgaata aatttcatca atccatttct caacttcctt cttgtttcct
    38281 tcaagttcgg ccagtaaaat ttccgcatac ttcaggctgt tcaggtccag gtatacatcc
    38341 acaagatcag gttgattacc ggagtctctt atctcaatat ctgtgaagct gaaggtgtaa
    38401 tcaccgctta ttttatcaag gtagttaaaa tataaatatt tagccagctg gtcataattc
    38461 atggatggat atactttctt gatgatatca ggcaatttct ttatactcgc aggagcaagc
    38521 attggtttca aatcgtttat aggtattgaa taattaatat tctgtgcatt ttcaatggag
    38581 gaatagttta ttcctacaac ctcaccatac atattaatta acggcccgcc gctgcttcca
    38641 ctggatatgg gtgccgtaat ctggatatcc ttttgcccgg ttcggtataa cacacccgga
    38701 gtattgaggc tgcttacatt tccgaatgat acagtgaccg gcaggcctaa cggatatcct
    38761 atagctacaa tatcatctcc caactggagg ctatcagaat ctccaagtat tactgccggc
    38821 aaatccttaa ctttttccag cttcaaaact gcaaggtcaa gttttttatc gtatgccgag
    38881 accccggcga cagcataggt ttcattattc gttgtaatga catctattct tggagccata
    38941 tcaattatat ggtagttagt gacaacttgt ccgtcggaag aaactataaa accgcttcca
    39001 tagccgacta cattattatt aatgtcataa attacgatta ttactgtcaa ggaagcttgc
    39061 actgatacct ctgttttgct taaaagtctt cttatgtttg ttattttcag actggcaata
    39121 tcggatactg tcccggaaga agcactgcct cttgcataaa tagttgcatt tgttgttaag
    39181 gccaccggct cggtatattc ctgccagggg ccgttatcga ttttgtattc tttggtatca
    39241 gcagtatccc agttgctgat tgtgaccata acagttttat cagtctgatc tgtgatatcc
    39301 ggtgtcaaaa ctggggttgc cgggatgatt ggttcttcat ctgcgtaaac gtttgataca
    39361 gataataaca gtaccagaat aagaataaaa aatataactt ctttatcttt tttcattaca
    39421 aagcccttcc gtttataata tttttccatt attaccatta caatattatt ttaccagata
    39481 taggctttga aataaagctt attattatgg tgcgttgcag cggaagctga cagaattatc
    39541 cgcaaataac ctactatgcg ggaaattcgc tatttggata agctgatcga tgagttggca
    39601 aagggaaaag cgatagatga gattttgcgg aaataatgcg ttctgccatc ctgtcagaaa
    39661 gattgatgct tagagtactt gcataagcct tcaccataat ttttttaatg tcatcaggga
    39721 cagccgcagc gttcacttta ttgacaaaag taataataag atttaccttg ctattgaatt
    39781 cactgatact catgtttccg cccacattca aaatattata tctcaatata tatattcgca
    39841 ggaggggaat tattgccggg attgtttttt atgtaaatta ttccaaattc gtaaggttca
    39901 tgttcagagc atgcaggagg gggcttgaca tcagtatgga tttgcttttg cggactagta
    39961 ttatattggc gtttctcaag gaattgatta cagtcggttt aatcattcca atttttctaa
    40021 gaaattgaag gaatgataaa agaagtgtcg aaatatatga ggaaatctga aatgaaagaa
    40081 ggtgtaacag atatggcaaa attcaacaac ataaaattaa gcaacaaaat gtctctatta
    40141 ctcacaatac ctattattgc tattgtaatt actacttata tttcagttac taatatagga
    40201 actgtttcag aagtattggt caaaaaactg tataatgaga tcaaccaaag cacggcttta
    40261 atgttaaatg ccgacaggga cttctatcaa gcccttgcag accagatgaa catgcagaaa
    40321 tcaacaaatc aggaggagct gcaaagatat agagaatcat acaatgagaa tgcaaagcag
    40381 gttgttgacc gtgtacatga agcgtatgga attctactta ggcaaaagag tgtatatgaa
    40441 aaattcaggc atcaggtttc aaataaaaca gtatttgagc ttattgaggc ttttaatagg
    40501 gactataata gatggtatgg attgttcaat gcaagtacca atgtcgtttc agatgaggag
    40561 cagtatataa atgcctttaa ttcggcaaga gagagtatga accagataga agaggtatta
    40621 gaggtatatg gggctgaaat tgttgctcag aataataaaa ctgtcttgca aacacagaga
    40681 tatgttgtcg tgataacagc tttggccgtt atactttctc tgctgcttgg aattattata
    40741 ataaccggca taagtaaaag aaccagaaaa actctggagc tgataaaaaa gactgccaat
    40801 cttgatttag aaaattcggc agaatatgat aaattcctga aggaaaaaga tgaattcggg
    40861 ttaataatca atgccgaatc tgaggtgcga aaagaattag taaatataat caaagaagta
    40921 agcaataatg catcaaccat aaacaaaata gttgatttga ccaatcaaag aatgtcacgc
    40981 ctgggagaag aaattgacgg tgtatctgct acaacggaag agctatcggc aggaatggaa
    41041 gaaaccgccg cctcagttca ggaaatgaat gcagcctcca atgaaatcga aagggcctcg
    41101 gagaatattg ccgaaaacgc caaagaagga tccttggaag cggaaaaaat cagcaagact
    41161 gccaatgaat tgaataagag ctttactgtt tcccaggaaa gtgcttacag aatattcgaa
    41221 gaagtaaaaa taagcctgga agaagcattg gaaagatcaa aggctgccga acagattagt
    41281 atattagccg attctattct ggaaataact tcacagacaa acctgcttgc cttaaatgcc
    41341 gcaattgaag ctgcaagagc cggagaagca gggaaaggct ttgcagttgt agctgatgaa
    41401 ataaggaagc tggcagaaaa ctccaagaat acggttacag agattcaaaa cattacacag
    41461 actgtattga gttcagtcaa tgatttatca tccagttcca aaagcttgct ggactttatg
    41521 aatagcgatg ttgtaaagga ctatggaatg atgcttcaag caacaaaaca atataaaaag
    41581 gatgctgaat ttgtaaacgg gctggtagaa aatctgagcg ctacggcgga aatgctgctg
    41641 gtctcaattc aaagcacggt taaagcgctt gatgaagtat ctgcagcgac aaatgaaggc
    41701 gccgagggga cgagcaatat agcacagcag acatccaaca ttgtggaaaa cgccggtgaa
    41761 gttattaaaa acatcgcaaa tgcaaaagaa gtatcggcca agcttaatca gatgatagcc
    41821 aagtttaaaa tataggcagt atgtaagtct gcatacaaat aaactcccat taagggagtt
    41881 ttatttgatt tctgcatgtt aagtatcaaa tgtattatgc tgccataaag cattggcaaa
    41941 agtgactcga caatacccca accggctaaa tcggataacc ccaatatttt aattagctga
    42001 tttttcatct atgtattcct tatccatcga taatatcatt gcttttacct tagattgtac
    42061 aggtaaaagt ataaaaacaa aaagtactga gtaaagcatt gtaaggaatg atactgccag
    42121 gtttgggcct atcttactta aattgcttaa gcttgccagg atagaaatca tgccgattat
    42181 gcttcccaat agccctgaaa acagcaatag cttaattgca agacttagag caatctctgt
    42241 cttattaagt tcaatttttg accaaagatt tactttatta cccattattt tgaagcctct
    42301 gatgaagtcc gtaaaaaggc cggatgccat tagcatggga atagtaaaag acaggataat
    42361 taatatgctg ggaagatcga taaaaaatgc aatggaaccc gaaattattg cagaaaatac
    42421 caagaaagca atgaaaagaa cgatacctgc aaaatacata aataattacc tcccgaataa
    42481 aaattatgtg tatggtatgt cttaatatta atccatatcc ggggtgaaag ccatactcca
    42541 gcggaagtaa ttaagaagta gttccggtta atatcgcgat gaaagcatca aatacagctt
    42601 tctgactgat gtgcctgccg ttatttttga gggttaaaat cgaagataaa aacatagaat
    42661 taattagtca ttatggctga atatttttgt ttcgggtgaa ggatttttgc cgatatgaag
    42721 agtattgata caaaaaagct gcataacagc aggttcgttg aaaatgccag aattgtaaca
    42781 gtttcaatac tactgatata tttgactgtt tcattatact ttatgaatca tttctttttt
    42841 aatactgtaa ttaacggagc agacgtttca ttaaaagcct ataatgaagc agacgaggtt
    42901 attataaggt atgttaaaga ttataagctg cagctgattg agcgaaacga aaaaacagaa
    42961 gaaattgcag gacaagatat tggaatgaaa ttcaatagaa ataacagtat ttctaaaatt
    43021 cataaaatgc aaacctcgtt taaatggccg atttcattat ttaaggaaca aagtttttat
    43081 gttaatgatt tatactttta taacagagaa aacctggaag acagaataga taatttggac
    43141 tgcctgaaca aagatatcac agagcctcaa aatgtgagct tcaaatatac aaacggctgc
    43201 tatgaaacag taaaggagat atatgggaat aagcttcata aaggcagatt atatgaagta
    43261 ataaaatcga gcatattaag aggacaacgg gaattggatc tggataaaag cctatgctac
    43321 ataaatccca agtacacctt aagctccgcc gagacttcgc aaactcagag cctgctgaac
    43381 aagtatgtat cggcaaaaat cacttatata ctcagaagcg agaaggaaat actggatcaa
    43441 aataccataa gtcaatggct cagtgttgat gaaggcctgg aggtggtaat agacgaaaat
    43501 gctgtcaggc gctatgtaaa gggattaagc aaaaaatatg atacagccgg tgctgcaaga
    43561 aagtttaaga catccgtgaa taaaacatta gaagtgaaag gcggttttta cggatggaaa
    43621 acagatattg ccgccgaagc ccaggcactg acagaaaata taaaacgcgg cgaagtgctt
    43681 gaaaaagaac ccatatacat acaaaaggct ttatcccggg atgaaaatga tataggggac
    43741 acttatgtgg aaataaatat tacaaggcag tatttgtggt attataaagc cggcaggctt
    43801 atagcacaag gccacatagt atcaggcaat cccaacaggg gaaacgccac cagtctcggt
    43861 gtctatatgc tgaattataa ggaaactgat actactataa gagggcccgg ttatgaagcc
    43921 gaagtaacct attggatgcc ttttaacggg aatataggaa tacatgatgc tgactggaga
    43981 tattctttcg gaggaaatat ctataaaagg aacggaaccc atggatgcgt aaatgttccg
    44041 ttatacttag ccaaaagaat ttatgaaaat attgaagtag gaacgcctgt tatttgttat
    44101 gaagagcagt gacagtgcgg gtatgcagga aatacaaatt ataactgaaa tggaataata
    44161 aagccgctcc attcaatgga gttttgtgat atcatgtata taacaaagac aagcttcata
    44221 agagaggatg aagtagagga tgagaaagaa atgcagtaaa actggcttta tttttgtatt
    44281 aataattatg atggttgcat tgactgcaaa cattacatat gctgattcgg atccgtccca
    44341 gccaagtaat tgggcaaaag ctgaaattga aaaagcaata gaaatcaatc ttctcccgga
    44401 aaggctgcag ggagaatacg gaagcaatat caccagagaa gagttcagcg aggttgctgt
    44461 gaatctgtat gaagccataa gcggaaaaga ggctgaattg caaggagaga atccttttac
    44521 cgatacgcaa agcacggaag tcaggatagc aaataatctg ggaatagtaa agggcattgg
    44581 agacggcagg tttgctcccg acaatgcgat aacccggcag gaaatcagtg tgatgatgta
    44641 tcgtacactg caggcggcaa aacccggata cgattataca aaaaagtatg aacatatatt
    44701 tgcggactat gatatgattt ccacatgggc acgggaggca gtaagttatt tatatgtaat
    44761 ggaggttgta aacggagtcg gagacaacag attcagcccc aagggaaata caaccaggga
    44821 acaggcggta gttcttgcaa aaagaatgca tgacaaagtt tctgcatcca aggataatct
    44881 aattgcgtca cgagatggga caagcagaca ggagaacgat ttaaaattaa aattggcaaa
    44941 acttatctcc caggagttgg gcaagccata caagtgggga ggtacagggc ctgacggcta
    45001 tgactgctcc ggattggtgt attccatata tggcaagctt ggcataccac tgccaagaac
    45061 agcgcgggat caggcgggag ccggcattta tgtatcaaag gaagcactgc agtatggtga
    45121 tttggtgctt ttcgcaaggg acggaaagaa tataaaccac gtcggaatat acgtgggaga
    45181 cggcatgatg gtgcactctc ctcaaacggg agatgtgata aagaagacaa caattatgtc
    45241 gggatattat gagagatgtt attatactgc gagaagggtt attgaataga aaaaaatgcg
    45301 ccgcaaggcg tatttttttc tattgctgaa tatttatttc caggcagatt tcattgcagt
    45361 taggaaactt ggggcaattt atgcagtctg tccagacctt ctgaggcata gtttccttat
    45421 ctacaacctt aaagcccatt ttttcaaaaa atccgggctg ataagtcagt gtaaatacct
    45481 tttgaagtcc cagttccttt gcttctttta atataaactc aacaatcctt ctgcctattc
    45541 cctgtctttc cataccgctc ctgactgcca gggtccgcag ctccgccagg tcgttccaaa
    45601 acacatgaag tgctccaatc cctgcgactt gaccgtctat ttcaataatg acaaaatcac
    45661 ggatgttttc acataaggca ctgcgggagc ggggaagcat caagcctttt tctgcataat
    45721 aattcactaa ttcatacatt tcttcaacat ggggcaatat tgccttacga ataatcaaca
    45781 caaacacctg ccttagtatt atatataatg catatcataa caaatgatgc ataatatttc
    45841 aatactaatg cataaaaatt ttgaaatggt taattattcc ttaacactgt tataaacccg
    45901 tcaagtattt gatggcttac tttaaaacct ttatgcacgt agaaattcag cgcattgtcg
    45961 tttccgtttg acacaaagat aaacaaatca cttattgcct taaaggtgct taaccagttc
    46021 atcgactttt caaataaagc acttcctatg ccggtattac gataatcttc ctttataaaa
    46081 aattgagaaa ggcagccgac atcatcggtt ttaacagaat caaaatcaaa gaaatctaca
    46141 ggcttaagag ttgcgaatcc gcctgaatat gtatatttcg aagaaatatt gctatacaca
    46201 taacctataa ttttatcttc atcctttgcc acaactatat agttggcttt ggcacttttg
    46261 acagacggaa tcactctggt ctcataagac atgttgtcaa accactcagg atgtatatat
    46321 gctttggatt tttgataagt catcaaatcg ttgcataaat ccttgatgca aataatatta
    46381 tcttcagaaa cttcttcata atggatactc atatttgttt tcctcctaaa aaaatgtttt
    46441 actgagcaat aagctttgat atcattatat cagcaactat tactataaca agtatgcagt
    46501 attttctgcc caagaaagga aaaactgagt aaaggagtga tactatgaat gaccaatcat
    46561 tattctggct gatttgttta ttgcggtaac aacgctgttt atggctctgt tgttcatgct
    46621 gggctataaa tccatagtac tgctttttgc aatgtctgct ttcagggcgg taggcaccgg
    46681 tgtacagaat ccggctgtgg gagcattcat tccccaaata gtacccgaag acaagctgac
    46741 aaaggtgaat ggagccaacg gaagtattca ggctgttata tcccttgttt ctccgatgat
    46801 cggcggggcg cttcttacta tgtccactct ggaggcaatt ttctttatcg atgtctttac
    46861 agcagcggcg gctgtattta ctctgctgct gttcctgaag gtgccggctc atgccaaggc
    46921 gcagaagccg caggaatcaa gctatttcaa ggatttgcac gagggtattg tatacatatg
    46981 gaatcacagt tatgtgaaga agtatttcat attctgtgcc gtattctttt tcatgcttgc
    47041 tcccatagca tttttgactc cccttcaggt taccaagagc ttcggaagcg atgtatggcg
    47101 attgactgcc gtagaagtcg ccttttctat cgggatgata ctgggaggaa tttttatttc
    47161 gtcctgggga ggattcagca acaggattta taccatggct cttgcaagct ttgtaacggg
    47221 tatctgtact cttgcccttg gagttgtacc gttattctgg gtatatttat ccataatggg
    47281 cataaccgga actaccatac ccctttttaa taccccttca acggtactgc ttcaggaaaa
    47341 agtagaagcg gatttcctcg gaaggatatt cggagtattg aatatgattg caagctcaat
    47401 gatgcctctg gggatgctgg tgttcgggcc cgttgccgat gttgtaaaaa ttgagtggat
    47461 gcttattggc acgggttttg tgatgtttat ccaaggcttt ttcctgattg gcagtaagga
    47521 ccttgtggaa gcgggaaggg caaaggatat aaccgggaca tgattcatta tcatttgttg
    47581 tagggtgact tatttaagtg ctataataat tgcaatacat atatagcggc taagaaagcc
    47641 ataaagctga caaataacta tcccaaattt tattgtactc tcgggaggct gcaggcaata
    47701 aaaggggact ttgaaaatgc aaagaaaaaa tatggatgga ttacggatgg ggctaccgga
    47761 ctcagggaag caaactttac ttcggataag accgacgcca ggtggtttgt gaaggcatgg
    47821 agcgaatatt tgaaaaaggc cttggagaat tccaatgaca gtttaaggta cattgttaag
    47881 aatggtattg gttcggttaa agagctatac cttaatgaag ttaataggga gaatatagat
    47941 aaaattgact tgccctcggc atcagccgga atttatagaa ttggggaagg catttttgag
    48001 tatttgctga ttggcgatat atgcccggtc atcgaagata ttgagggcaa tattaaagaa
    48061 attaaggata caaagctgga agtactggat aatatagctg ttaatgaact gagacggctg
    48121 atggccagcc gggggcttag ctttaatgag gccagggagg gtatccagga gattcttcta
    48181 aagcacaggc ggcttaaaaa cactgataaa ggatattgga ccctggaatt tgagccaagc
    48241 gccgttgact attgcatcta tgacaagctg cctttggagt ctataagcaa agctttgttt
    48301 atgaccgacg gattttttgc cattcattca agctataatt atttcagtgt taaggattta
    48361 atatcattca cagatatgaa agggctggag aaagtatata aaactctgag ggatattgaa
    48421 gaagaagata aagagtgcaa tagatatccc agatttaaaa aaagcgatga tgcctcggca
    48481 atatacatta aatatgagga tgtgcaggct gtttattgat tgctgtataa tgcctggtat
    48541 gacaatactg ccatattggt ttttgttttt tgagtgttga aattttgagt agggggttaa
    48601 ataaagtgag ttatttcgat tcctatagag gattgcccag aagtgtttac ataatatttt
    48661 ttgcacagat aatcaaccgt tttggtgatt ttgtactgcc gtttctgagt ctgtttctgg
    48721 ttaaaaagct tgggctgagc tatcaagacg caggaattgc ggttatgctg acttccctgt
    48781 caacaattcc gggatctttt gcagggggga agattgcgga tcatttagga cgcaagaagt
    48841 cttatgcatt cttccaatta agtgcggcag tatgtttgtt cttttgtgcc ttttcagaaa
    48901 atccgcaatt cattatagct cttatatgta tggcttcatt ttctaacgga gcagtccgcc
    48961 cgataatatc agcaattatc actgatgttt tgcctgctga aaagagaaaa atgggatttt
    49021 cattatccta tatggggata aatcttggcg tttccctggg gccccttgtc gcaggctttc
    49081 tgttcaaccg ttatattccc cttatattta tcggtgatgc ggtaacttca ttgatagcag
    49141 ttattctggt tttgacaaat atcaaggaga cattgcctga ttactctgac aataaatgct
    49201 taaacgaaga agaaaaaagt gaagaaggca atgttttttc cgttttgata agaaggccca
    49261 aaatactgat ctttcttacc atcaatatag tttttaatgt tgtatatgca cagcactctt
    49321 tttcgctgcc tatgatgctg gataatattt ttggcagcag cggggcgagc aactttggag
    49381 ttattatgag ctgcaatgct ttgacggtca ttatactgac aatgttgata gcccatgcta
    49441 caagtaaatg gaagccttta agcgctatca ccatagcagg gctgctgtat gccataggct
    49501 tcgggatgat aacctttatc agctcaatgc cgttgtttat tatttccacc gttatctgga
    49561 caacaggtga gataatgacg gtgacaaact ttggagtata tatagcaaac aatactccgc
    49621 aaaatttcag ggcaaggttc aatgcgatat cctctttaag ctgggctgtg ggggctgctt
    49681 tgggcacatc agtaatgggc aggtatatgg acttttttgg cgtaaaagcc gtatggcctt
    49741 tggctttctg tctggggctt gcaggtgcag caggcatgta tctgcttgaa tctcacagta
    49801 attttagagt aagcagggca aaggaactca attaatgata taataatttc gttggatgat
    49861 aagacgctgg gggattttta tgaacggtga catgactcaa ggaagttcgt taaaagcgct
    49921 gattaccttt tccgtacctt tgatgctgga gaacatattg cagcagcttt attatataac
    49981 tgatgctgtg atattgggca gatttgtcgg caaaaatgct ttggcagcgg tgggagtggc
    50041 caatcctatc atgtccactg caattttctt tatgtttggc gtatgtattg ggacagctat
    50101 atttatgtct cagatttttg gggctgcaaa atatgaaatc ttaaagagag aagtatcaac
    50161 ggctttaatt gccggtgcgg tttttaccgg tttattatct ataatatccg tactttcagc
    50221 aagatatatt ctaattgcta tcggtactcc ggaagagatt atgaatgatg cggaaatata
    50281 cctgaagata ttatccggag ggctgatatt ttctttcttg tacaactttt actcttttac
    50341 attgaaatct attggggatt caaaaacccc attcattttt ttaagtatat cctgtgttct
    50401 taacggaatc ttatgctatt gctttgttgc tttgcttgga cttggagtgg ttggatcagc
    50461 agctgcaacg ataattgcac aggttgtgtc ttctgtaatg tgcatatcat atgtttacat
    50521 aaaagttcca atgataagcc ttaagcctaa tgagttggtt atggacaagt ctttgttgag
    50581 aaagacaata aaatacagca gcattacggc attgcagcaa atcagcctgt atatggggaa
    50641 actgctggtc cagggagttg tcaatccctt tggaacaaac accatagcgg catataatac
    50701 agtaaccaga atcgatgctt ttgtgctttc cccaggtgac aactttgtag tgtctgtctc
    50761 tacttatact gctcagaaca tgggagcaag gaagtggaaa aggataatag agggctataa
    50821 aatcggcaat attattatta caatatataa tcttgctgtt gccgcgattg tattttttgg
    50881 agcggagaaa ttaatgcatc tgtttatatc cgcttctgaa agagaggtta ttatgatagg
    50941 agtggagtat ttaagactaa tgccattatt ttatgtgctt acaggcttct gcaatatctt
    51001 tcagggtatt ttcagaggaa tcggaaagct tcatataaca tttttcgcga cagttacgca
    51061 ggtatctgca agagttattc tggctttaat attcgccccc tatttcggca tttcgggcat
    51121 atgttatgca gttggtatag ggtgggtaat tatgattata tatgagggat taatatatag
    51181 aaaggaatat ttgaggatga aggcatttga tactgcgcaa gatgttcttg attcggcttg
    51241 ataaatatga aggagggata aaggtgattg attatgaggg acagtatccc gtatatgaga
    51301 tttcagcctc aaaaataaaa agcaatgccc gcaaaatata tgagatgtgc agggccaacg
    51361 ggatcgagcc ggcaggtgtt gtgaagggct tcagcgcaat accggaggtg gcggaattat
    51421 ttatagaggc aggctttaag acgttagggg attcgaggat gaaggagatt attaagctcc
    51481 ggagggccgg tatcaaaggg gagcttatgc ttatccgaat acctatgcca tgtgaaatac
    51541 cggagcttgt aagctttgca gattcaagcc tgaactccga aatggaaaca atcaggctta
    51601 tcaacaaaga agcaggcctg cagggaaaag ttcacggagt catattgatg gttgatttgg
    51661 gagacctgag agaaggcttt tttaacaggg aagaggcaat tgaagccgcg aaggaagttg
    51721 aaatgctgga gaatgtcaag ctgttgggaa tcggtactaa tttaggctgc tatggttcca
    51781 tcaagccttc tgaaaaaaac ctgagagagc tgtgccggct ggcagagtta attgaaaaag
    51841 aaatcggaag aagccttgag ataatatcag gcggagccag cacctcatta tcgttattag
    51901 ttaatggtaa gatgcctgaa cgaatcaacc acttgagaat aggtgaggca atcattactg
    51961 caaaggattt tccctatgtg tggggtatag aaatgccggg gatttaccgg gacccaatgg
    52021 tactgaaggc acaggtggtg gaagtaaaaa cgaagccaag ctatccgata ggggaaatat
    52081 ttgtggacgc ttttggcaac aggccggtat ataccgacag gggaatgcgg aaaagagcag
    52141 tactggcggt agggaacaag gatgtcggcg acttttcaaa gcttaagcca aggcatgaag
    52201 gaattgagat aatagggagc agcagtgacc atctgatact ggatattgag gattatgcag
    52261 gggatatcaa agtaggggat gttttggaat ttgatacctt ttatgcggca atggtctttt
    52321 tgtgctccag tgattgtgtc agaaaggcag ttgttgagtg aacccctgct gatatgaatg
    52381 gtattcagag caaaataagc aggaatcggg cacttaagtg aattgaatcc tgcttatttt
    52441 tacttcaatt aatgttctac cagactggtt acaggcactt caagcttatt tgaaggttga
    52501 tcaaggggat gaatatcagc aattccggtt tcttcgtcca cattttcaat atatatcggt
    52561 attccgttat aggttacatg agtcataacc ggtgaagcag taatggcttt tgcacgtctt
    52621 tgatccatag aataaacctc ctgtattttt tattaaacat agtttttgca ttaaaaattc
    52681 aattattcct attgaagaaa taatttcatc aggaaattac aactgtaatt gcagacagca
    52741 aaaagtgccg ctaggcattg aaattactag attcttaatt gcgcaaaaga attatttagc
    52801 gcaatgtgac acaatattac aaaaaaccgc atagttttta ttcaatcaca tttttcctca
    52861 caattaagtt taatattgca atttagcttg atttttatta tcaagtatga cattattaat
    52921 aatatcttaa ataattagta aataatgtaa cacatatata tttcaataag tactaaaaat
    52981 atagtaaacg gtagttgaaa ttttgtggtg agttatgaca aagtatttga gaaataagat
    53041 tttggaggta cacaagtatg gttggtacag taaaatggtt caatggtgaa aaaggttatg
    53101 gatttattac aggagaagat ggcacggatg tctttgcgca tttttcccag ataaatgcag
    53161 agggttacaa atcacttcaa gaaggacaga aagtatctta tgacgtaaca aacggaccca
    53221 aagggcctca ggcagaaaat atcactatta tataaggttg atattttacc ccttgacagc
    53281 atggctgtcg aggggttttt agttaccaaa ggacacatga gctatatgcg gctgcagccg
    53341 catttttttt gtacaaaatt gggggtattg attgtacggc ttgttttgca ccaaacttgt
    53401 caggtctgta tatactgtaa tgaaaagaaa aaggaggtat agcttgaaag taaaaggttt
    53461 aattatcact gcttgtatag tctttgcctt agcttctttc ctaccgggat gttctgtatc
    53521 atcaagcaat aatacaaata ttaatcagga gaaggaagag cctaaggtgc ttacaatata
    53581 cagcggcagg caggaaaagt tcataatacc gctggttgag aaatttgaag aggataccgg
    53641 aataaaagtt aatcttattt ccggaaaagc aacagaatac gcccacagaa taattgaaga
    53701 aaaggaaaat actcaggcag acgtattttt atcaaatgac ggcgagatat tggaatatct
    53761 ccgcatagaa aatatgctgt caccgattaa ttcaaagctt ttagaggaag ttcccgggaa
    53821 ttatgttggg aaggataata cttggaccgc tcttaccata agatatcgtg tgtttatata
    53881 caatacggat ttaataacat tggacgaaat gccggactcc atttttgacc tgacggatcc
    53941 aaaatataaa gggcaattta ccatgaacag agcaggaaat gaatcgatga ttacctactt
    54001 tgcttctatt cagttaaaat taaaattatt atagtataat ataattaaga taaaaacagt
    54061 atagaggata tacgatggga ggaaatatga aatgataaaa gtagttgcaa aaagctttgc
    54121 aaagccggac aagcttgata agattctgaa gctgtcggca gagatggtgg aaaagaccgt
    54181 taaagaagag ggctgtataa agtatgagct ttttcaggat ataaaagctc ctgaagtact
    54241 tgttatcgtt gaggaatggg aaagcgaaga ggccttgagc aggcatatgg cttcggagca
    54301 cttcagaaga atagtgcccc agttgaatga gctgagggaa aaagcatcgg aagtcaatat
    54361 actaaaaaaa ctcttttgaa ataatgtttc aggccccgcg gcgacatttg ccgcggggcc
    54421 ttttatttta tgaattctgt tgtgaatttt gctgctgatt ctgctgctgg ttgttttgtc
    54481 ctttgcccat tatctgaggc agctgattta tcagttggtt cacattgccc ggattggcgt
    54541 tgaccggcag gatggagaca ttctcaccat ctaccagtgc caaagcatcg gtactgattc
    54601 tggcaccaag gcccagagca tccattcctg cattgccggt tccctggttt gagcctgcag
    54661 ctcctccgga cattttaccg gagctattgc cgcctccgta tccgattgtt acggagataa
    54721 tgggtattaa ggtcttatcc ccttgggaaa caggctttcc caacagtcct tcattttgag
    54781 taaaggtttc aatattagtg aacagtgcat caatattctg ggttagagaa gtattaacct
    54841 ccatagtttt cctccttaga tataattact tgtcaaataa gcttcattaa taagcggccc
    54901 aagttgagct ttgcagtggc atccagattg atatagtcgc tgtttgccag aaagtccggt
    54961 ttttgatgca gggattccac atttaataac tgtgaaaccg cacttactgc accgcaggcc
    55021 aggcctgtaa caaaaggatc ccggaacccg tattgagtat tcacatggat ttcggttggt
    55081 ttaagacttt tgagaatatt gccgctcctt ttggtatatt gttttggttt cattaatttt
    55141 gctaaaaccc tttttttcag aagatataca agtaagagga atccattgcc ttccttaact
    55201 atcactgacc taagcagagg atgaagccat cttaatgtca ggtgcatatc tgatctggca
    55261 gagtcatagc ttagatcgat tcttgatgcc acggtaaaca ataaaaaaat aaaaagcaga
    55321 agagacagta acagtataaa cagtaaagtc atggaattaa cctccatgta cgcatttgtt
    55381 cagcctttaa tagtttaacc atttcggact tatttactgt gtcagaatat actgcctctt
    55441 acatatatga ctatacaata tcctgtcatt tatttttttg cttatatgaa ggctgccgtc
    55501 actttgcaat tcagcctggt atcagcaaaa tcgcagtaaa aaaacaaaaa aataaaagag
    55561 cttcaaaaga agctcaaaga acagcaatgt gcagccgcta tattccggac aatacctcca
    55621 gaacttcatt aatatccggc agctgactga taggatatac cttaatccat ctgacaattc
    55681 cgttttcatc tataataata tttgctcgct ctgatattcc ttttttttct ataaaaatgc
    55741 cgtagtcccg ggcaacctgt ccatggggcc agaaatcgga aggaagactg acgttatcaa
    55801 ttgcaattac tgcactccag gctttttttg aaggtaccgg gtccacactc aggccaaggg
    55861 gtacggtatt gaggctatgg aatttctccc aattgacttc caaggacctc atctgatcgg
    55921 tgcaaacgga agtccaggca agagggtgcc atgaaagcaa cacctttttt cctctgaaat
    55981 cagataagcg tattatttgc tccttgttat ccttaaggga aaaatccggt gcattatctc
    56041 ctattataat actcactaca aactcatctc ctttcttgat tatgtattaa tcataccata
    56101 agtattgtat cagggaaaca gggggtttgg cacaaagttc ctatattgtc atattatatc
    56161 gatttaaggt ataataagag aaaagaagac agaaagaagg ttgacagtat gtttaaaaga
    56221 tattttaaag tatgtcagct gttcttagat tcatatcata aaggcttgtt atgggctagc
    56281 cgggagactg gcttcgtctg ctgcgtatcc gtgaactaca tggactggaa aatatactca
    56341 atggaagaat ataaaaactt cgtaataaca attgtgctta tattctcgct tcttattata
    56401 tccgttatta tgcttgcagc ctatataaac agaatcagca agataaagag agagctgcag
    56461 ataaaaaatg aagagcttac agcatccgac gataagctta aaaagcagct tgaagagcat
    56521 gagagagttc agcacagttt gataaccagt gagaaaagat accttttact gtttgaaaaa
    56581 atgatgaacg cattttttat atttgaaccg gttattgacg aggaaggaat tatttccgat
    56641 atacgctttc ttaaggtaaa tccggggttt gaactccata caggtctgaa aggagctgaa
    56701 ataatcggta agaaatggat ggaggtatat aattatccca ataaaaatct tcatatatac
    56761 catagaattt tgctgaccgg aaagcctgaa cattttgaaa cctactatga acaggaggat
    56821 aaatattatc tgaccaatgg atttaagata tcagacaacg aagttggggc aatatttgaa
    56881 aatattacac gatacaaaaa ggcaatcaaa gaaataacaa tgcttaatga agaactggag
    56941 cataaggttg cagagagaac cgacgagctg caaaacgcgt taaacgagct tgaagctttt
    57001 acatatacag tatcccatga tttgaaatca cccctcagag ccatagaagg ctacagcaag
    57061 tttgtaattg aagatctgga atcgggaccg ggcgaggaaa cggcggagat gatacataat
    57121 atcaggaata tcagcctgga tatgatagaa atgataaaca agctgcttga atattcaact
    57181 atgactaaga ctgaaatagc aaaggaaagc gttgactcag gggagctgtt caagtcaata
    57241 attaatgaat tattatcagt caatacagac agaaatatag agctaaaggc ggtaacggag
    57301 ctgcctgttg tgtttgctga caaagtgatg tttaagcagg ttctgtacaa tatactgtcc
    57361 aatgcggtaa agttcacaaa acttaaagac cgggctatta tcgaagttgg ctgctcaatt
    57421 aatgagggag aatacatttt ccatgtcagg gacaatgggg ttgggtttga tatgcaatat
    57481 tccgggaagc tgtttggcat atttcagaga ctccatacaa aagatgagtt tgacggaagc
    57541 ggtatagggc ttgttacggt aaaaaagatt atacagaaac acggaggcag ggtttggata
    57601 aaaggcagca tgggacaagg ggcaacagta tattttacat tgcctgttga aagaggtgga
    57661 atatatgctg caggtaatga ttgtggatga tgtggatatt gtaagaaaag agctgaaaag
    57721 gctgaagctg tggggtgaca aaacaggctt tgtcatctcc ggtgaagcat gtaacgggca
    57781 tgaggcattg gaaaagctcg aggaaagcaa gttcgatatg gttattacag acataaggat
    57841 gcctaaagta gacggtattg agctgcttaa agaaataaca gggagaaagc tttgcccctg
    57901 tgttgtactg cttagtgatt acagtgaatt tgtgtatgcc agacagggat tggtattggg
    57961 agcttttgac tatataatca agccggtggc agaagaagaa ttggaaaagc tgcttcaaag
    58021 agcaaaggaa tatatatccg agaaaaggct tgaggagcaa aggattttac agctggagca
    58081 aaagctggtg gaaaaggtgg atatgttttt cccgcagtcg gaggttagcc agcttattga
    58141 tgccataatg gcaggacata caaagactat tgaaaatgca gggagcatat ttgaaatagt
    58201 ttgtacaaat atgaattgtg atttgattaa ggtgaaaagc ctgctaaaca gtataatgct
    58261 tgaaataacc aataaaatat cagcgagctg caaatggctg gataaatata cggatattaa
    58321 tgctataaga acgattgatt tttccggagc aaatgatttg aattcaataa aaaacagatt
    58381 tgtttcggct gttgggagaa tctccggctt gtttgcgaga ttgaaacacg gcacggagga
    58441 taacgggatt acaggtcagg tatgcaggta tgtactggag aatattgacg gaaatataac
    58501 gctgagtgca gtatccgaat cccttttcat gagcaaatcc tatatcagcg aggtttttaa
    58561 gcagaagaca ggcctatcct tcatagagta tctgactatt gtaaaaatgg aaagggcaaa
    58621 aaagcttgtc ggggacggaa ggttaaagac atatgaggtg gcagagctta tggggtttaa
    58681 ggatattgag tatttcagca ggttgtttaa gaaatataca ggtataacac ccaccgaata
    58741 tcgtcagggc atggaaggag aaaaatataa agacccgaaa aaattcgggg tataaaccca
    58801 taatttttca ttcatgaaat aaggataatt aagtattatt atacagtaat actgattatc
    58861 cttattttat aggagacaaa tatgaagcag attgatgtat tggtggtgga agactcattc
    58921 tattcagcag atttgagtat aagggtatta aaaaaagcgg gattcaatgt tcggtataaa
    58981 attgcttcaa gcagtataac tatgcgagaa gctcttaagg accggcattg ggatcttata
    59041 ctaagtgata attatatgcc gggttttgat gcacttcagg ctattgagat cagagaccaa
    59101 tcaggaagcc tggctccctt tgtgattgtc tcggagtata ttcaagagat cgatatcagg
    59161 aaagcaatgg aaaaggggtg ttgtgcttat atagccaaag aggatctgga taagctggcg
    59221 cagcatattg aaaaaatttt ggaataataa atttagaaat ccattgatgt aatatttgtg
    59281 tcacaggagg aactaatggt ctttaataaa aatgaaaaaa aacttgaaag gattgaaaaa
    59341 gtgacttttg gtaatgagta tcgggaattt ttaggaaagg gtgagttgga atccaatcag
    59401 atatcattaa gaatagcact tacttatatg attgtagtgg cattgtggat acttttatcg
    59461 gataagatac tgggattgct tgcggcggat aatgcagtca taaccaggat aagcatgata
    59521 aaaggatggg tttatgtatt aatatcaggc atattgattt ttgttatagt cagttattca
    59581 cttaagagag tgaagattgc agagggaaag ctttatgaca gctgtcagaa cctgagtgaa
    59641 gcaaatatgg aactggaaca ccttgcatac catgaccaga ttacaggtgt ccggaataaa
    59701 atttcactga taaagcgtat taatgagctt gtcagttcag atgacagcaa gaagattgca
    59761 attcttattg cagatattga taattttaag tacattaatg atgcaatggg gcatagcatc
    59821 ggcgatcaag tgctgattaa attcagttct cgtcttaaag gcttggctgg agtggattct
    59881 accgtataca ggcttggcgg cgatgggttc gtaatagcct tggagggcta tgaggaggca
    59941 tcttctgtta aaaggctggc agaaagtttg ttgaaggact ttaagacccc ccttgatgtt
    60001 aacggcagta aattattcat tacaataagt ataggagttt caatgtatcc ggaaaatgga
    60061 gacggtatgg atgaacttct taaaaatgcg gatattgcat taaataaggc aaaagcaacg
    60121 gggaagaaca gagtggtttt ctataataga accatgaatg aagcagtatc agagagagta
    60181 aacatagaaa agcatttgag agcagcgttg attaataacg aatttgaatt gtattaccag
    60241 ccccagtggg atattgtcca aaataaggta tcaggcctgg aagcgctgat aagatggaaa
    60301 aaccctgaac ttgggtatgt aaatcccctt aaatttatca acattgctga ggatacacat
    60361 atgattattc ctataggtga atgggtattg aaaagtgctt gcacgaccct gaaagaattg
    60421 cacaggcaag gccatgaagg gctttccgta tccgtcaata tttccatggt ccagttgctc
    60481 caggatgatt ttgtatgcaa agtaaccggt attcttgatt atgccggctt gagtccggag
    60541 tatcttgagc ttgaaataac ggaatcagta ttgatggaat cctgtgatgc attggaaggg
    60601 aagctcaaga ttctgaagga catgggaata aaaattgcat tggatgactt tggaaaggga
    60661 tattcctcgc ttaactattt aaagcagctg ccgatatcca ctctgaagat agataaatca
    60721 ttcattgaca cgatagagtc cgatagtaag cataaatcct tgacaaatgt tattgtaaag
    60781 ctgggaagaa tcatgggact gagtgtagtg gcggaaggtg ttgaaactaa ggagcagttg
    60841 gattatctta ccaggcacag gtgtgataag gtccaggggt atctgttcag caaaccggtg
    60901 ccgggggaaa aggtcatcga agctataaga gggctgaacg tcagttttcg ctgatcagca
    60961 aaaatatatg aaatatttat taaaaagcgg ttcgtgaagt gtaccgcttt tttttcggca
    61021 aaatgcatat gaaacttatc agccttataa tcactctatt attgattttc actgtaatgg
    61081 tgagatgtac gctgcagaaa gccggaaaaa cttttattaa aacagtaagt ttggaaaaag
    61141 aaaacattca gcaagaaaat ttgatgaatt attattaagg aacctcttat ctttgttgac
    61201 agtgttgggg aaaattaata taattaccgt aagtatggga cagttcctga ctgtcccaat
    61261 ttactgaaaa ggacggagtg gatcggatga gtaagagacc tgttgtattg gttgttatgg
    61321 atggcatagg aataaatgaa gaggaaaagg gcaatgcagt gaaggctgca aatacaccga
    61381 ctcttgatat gcttatgaat cacagtccgt atacattgat aaaagcccac ggcacggccg
    61441 ttggcctccc ttctgatgag gacatgggga attcagaggt aggtcataac gctttaggca
    61501 gcggacagat tttcagccag ggggccaagc tggtaaatga gtcaatagag aatggaaaaa
    61561 tgtacagttc cgatacatgg cgaaagatag ccggaaattg tgttgctaac ggctccgcct
    61621 tgcattttat cggactgctg tcagatggta atgtacattc caatattgcc catttgaaat
    61681 caatgattat cagggccaag gaggacaaaa tcaaaaaagt gagggttcat gccctgcttg
    61741 acggcaggga tgtactggca acatcggctt tggattatgt tgaacagatg gaaaagctgt
    61801 tcaaggaact gaacgacggc agctttgacg gaagaatagc cagcggcggc ggaagaatga
    61861 agattaccat ggacagatat gaggcaaact gggatatggt aaggcttggc tgggataccc
    61921 atgtatcggg caagggcagg cagtttggtt ctgccagtga agcaatatgc atatataggg
    61981 aggaactccc cggggtaatt gaccaggatc tgcctccttt tgtaattgca gaaaacggca
    62041 ttccggcagg tactatcaat gacggagaca gtgttgtgct gtttaatttc agaggtgaca
    62101 gggcgttgga attgagcatg gcttttgact atgaggattt tgacaagttc gaaagaggca
    62161 ggtaccccaa ggttatattc tgcggtatgc ttcagtatga cggtgatttg aagctgccta
    62221 agaatttcct ggtctctcct ccgaatatta agaatacatt atccgagcat ctggttaagc
    62281 acggtataaa gcagtatgca gtatctgaga cacagaagta cgggcatgtg acatacttct
    62341 ggaatggcaa cagaatcgag aagtttgacg ataaacttga ggtttttgag gagatacctt
    62401 cagacagggt atcctttgat gaacgtccct ggatgaaggc tgcggaagtt acggataagc
    62461 ttatagatgc cttgaaatcc ggcaaatacg acttcttgag agccaattac cccaatggag
    62521 atatggttgg acataccggc agctttaatg ccaccagaat agcagtggaa gccgttgacc
    62581 tttgccttgc aagagtcata aaagcagcgg atgaggcccg ggccatactc atcattacgg
    62641 ctgatcatgg aaacgcagat gagatgcttg aaaaaacaaa aggaggcact gtaaaggcaa
    62701 aaaccagcca tacactcaat ccggttcctt ttataataca cgacaaagtg caaaggcacg
    62761 aaataaagga agggagcttc gggcttgcaa atattaccgc aactataaca accctgctgg
    62821 gaatcgacca cccggattgc tgggaagaaa gtatagtata aaaaatctat tgacaaataa
    62881 taaaatacgt aatatattac ttaagtcggt aatatattac gtatttttta attcctcatc
    62941 tccaacatat tggaagagac gctgaaagat gccggacaag gcataataag ctttaagctt
    63001 aagaatatga atataatgcc ctgcaggtca tgaggagcct gcggattccc ggatataacc
    63061 aggatagagc ctctgctgaa ctgctgcagg ctatttgccg aagaaactga aatgaggtgg
    63121 cttgggggcc ttgccttcgg aggggggccg ttgataaacg gagcttttat accggccctt
    63181 ggcggcttac atgaacaata acgccggaaa gcaggcacgt cttaacggaa atatcgatta
    63241 tgcaagaaag gtttatcttg aataggccaa aaacagtaat atatagtcac gtataagcat
    63301 actttgacta tacattactg ttttttcttt ttattatgtc agtgcactcc gtcggctatt
    63361 ttacataatt atcaaagtag ccctgaatat acacaatagg ggtaccttta tccccgctgc
    63421 ctgaggtcag gtcgcagagg gagcctatca ggtctgtaag ctttctgggg gtggtgcctt
    63481 gggcctccat gcagcctacc aggtttgagc ctttgcacct aatgtattca gatacggctt
    63541 ttttcaattc ttcccccttt aaatgagcaa agttattatc ggcaatatac ttcagtttta
    63601 tttcgttagg cgtcccttcc agtccggagg tatatgcagg ggataccaca gggtcggcaa
    63661 gctcccaaat cttgcacacc ggatccttaa aagccccatc gccataaatc ataacctcca
    63721 ctgtcctgcc tgtcttttct ttcagcatag cttgaatttt ttcaactaca ggctggcagt
    63781 tgcgggggaa gagctttacg ctgctttccg ttgatttgtt tgaacccagc agtccgtata
    63841 cctcattgaa gccgctgccg ccaacgggtg aagacataat gtcatccaag ccgtatacct
    63901 tttctccgcc gttttccttc agtattcttt ttgtcctggc tcttgtgtga atatcgcagc
    63961 agagtacatt ttttgtatgc ttgagaatgg ttaaggggtt gttggagaat attatttcac
    64021 actcaatgcc ttgttcgttg attatggatt tataatattc aatataatca acaccggtaa
    64081 aggtatgcct gctgtatccg aagtatcggc gaaaatcacc ttctgtcaat acatccgacc
    64141 agggattcag gttcttttcg ctgaaggtgt ctatatctat caattgattt cccacttcat
    64201 cggtgggata actgaacatc agtaccgctt tccctgctcc ttttgcaata cccttaaggc
    64261 atatggcaaa ccgattacgg ctcatgatag ggaatattac tccgattgta ctgcttccga
    64321 accttgattt aatatcttct gcgatatcat ctattgatgc aaagttaccc tgcgcccggg
    64381 caatcaccga ttcagtaaca gcaacaatat cacggtcatt aatgctgaaa ccttctgatt
    64441 ctgaagcttt taatacagta ttcacgacaa tatctgctat attgtccccc tgcctgataa
    64501 taggggcacg gagccctctt gaaatagtac atgttattct ttccataaaa atctctcctc
    64561 aaccgtatca gattatcgga tatatagtgt ttccatacta tactatacaa tagcatttga
    64621 cataagtaaa attaaaacta tttgtttcaa caattatatt tacaaaaact gcaggaaact
    64681 gttatgtttt tcatatttat tatgttataa taaatattgc ttaagatctg ttatattcct
    64741 tccggataaa gtgatatcag gtacggagca tcagcgtaag aagggtgtgc aggtattgag
    64801 gggtattagc cggtaaaaag ggaggaataa aggaactatg attgagaaaa ggagacacaa
    64861 aaggcttcct ttaaaactgg aactgaagat ttcttcattg ttcaggcagg attatgaaat
    64921 aattgatgat attaatgagg gtattgagat taggaatata tctaagacag gtataggatt
    64981 tttctgtact catgagctgc cgctgaacta ttattttgat gcgaagatcc agcttacacc
    65041 ggatagccat ttctatgcag tagtgaagat aatacggatg gaaaagcttg aggaaggata
    65101 ctttgcaggc tgtgagttta taggattggc agatatattg agcatgaggg ttgatatgta
    65161 cgaggatgaa ctgaataata tttagattcg gggcggtatt tattccgccc cgtagttatt
    65221 aaattattca agggggttct cgattagtgt ggcaaattga aaatcatgat cgtggcaggc
    65281 gtcaaaggta gtcgtatcac tggcgaagtg aacatgcctg ccgccgtcga ccggaacctg
    65341 aaggcctgtt gttcctccta ccccgtgtat atgaccgaag aagaagtctg tatcggtcat
    65401 gaaggtgtgc acatgtcctc cgggaacaaa tatcacttca ctggtgactc ctgcaaatct
    65461 gtggttatgt gcttccacgt cttgtgaagg aagcattgta cttcccaaga attcatgatt
    65521 atgggtctga gtctcctcga tgaatttgta ttcatatgag gattctttct gctgtacata
    65581 ttttttttct ttttttgaac cgaaagttac cattggcatc attatttccc tcctttcgct
    65641 gcaatatttt taatataacg gtttgtccca atacacaata tgcagcagcc tgagggatgt
    65701 tactgttaat taagaggaca cgggggacac ggggacgttt ctcctgtccc gctttcagcg
    65761 ggacaggaga aacgtccccg tgtccccccc gtgtcctctt aaatcctgtt agtctttgta
    65821 atttgaaagt gcagtacttg tggtgttaat tgcattttct atggcattca gggtattttg
    65881 aatctgctgc ttgttttcag gtttttcggc tgaattaaga gcctgattca atgaggtttt
    65941 agcattgtta agctggctga aaacctcctg tacttgctgt tttgcattct ttcccgacat
    66001 ttattcgtac ctccgtatgt ttataatttg atttacaata ttattatctt aattttgcag
    66061 gaaataatgc ttgaactaaa tggatgttaa gacaattcct atcaagttgc cacattgtac
    66121 aacaaatgtt aaaataagct atgatgtata taaaattgcc cggagggata taatatgaag
    66181 ctgatttcat ggaatgtaaa tggtctcaga gcctgtatac aaaagggctt tttggaatat
    66241 ttcagagaag cggatgctga tgttttctgc attcaggaga gcaagctgca ggaggggcag
    66301 gtggatttga aaattccagg ctatcatcag tattggaatt atgctgagaa gaagggctat
    66361 tcaggggttg ccgttttcag caaagctgag ccgttgaact tcacttgcgg aataggcagg
    66421 gaagagcatg acaaggaagg aagagtaatt actcttgaat ataatgatat gtatattgtt
    66481 acggtatata cccccaattc ccaggaggaa ctggcaaggc tgggctacag aatgcaatgg
    66541 gaggatgagt tcaggagtta cataaaggga ttggacggca agaagcctgt tgtggtctgc
    66601 ggggatatga atgtggcgca taaggaaata gatttgaaga atccgggcag caacaggaga
    66661 aatgcaggat tcaccgatga ggaaagaaac aagttcagcg aatttttgga agcgggtttt
    66721 atagatacct atcggtattt ctaccctgac agggcaggtg tttactcctg gtggtcatac
    66781 cgcttcaatg caagagctaa taatgccgga tggcgcatag actatttttg tgtttctgaa
    66841 aggctggccg agaggctgat cagcgcggat attcattcgc aggtactggg atcggaccac
    66901 tgtccggtag aattgattat gaaataacga ggcgaaaaga tatcctccat ttctataagt
    66961 tgtgggtacc gttttcgttg tccattaggc ggttaacatt tttcccgcct cgttgaaacg
    67021 cccgggtagg tttttgcaag tgtgcaaaaa cccttattga aatcttgtta taacagaaca
    67081 ggggatgcat atgattaaaa cttttgatga gttgggaatg gatgagcgtc tgattgaagg
    67141 cttgaaaaag caggggatca cggaaccgac accaattcag gccgataccg ttaaactagc
    67201 tttggaaaac aaggatatag tcggacaatc acctacggga agcggaaaga ctctggcatt
    67261 tctgcttcct atttttcaaa gggtggatgc cggcaaaaga gagaaccagg caatagtact
    67321 ggaaccgacc catgagttgg tgatgcagat agaaaagcag gcaaggctgc tggctgcaaa
    67381 ctcaggcatt gccgttactt ccgcagcaat tataggagaa gtgaacatca cccggcagat
    67441 agaaaagctg agagagaagc ccaatataat cataggttcc gcaggaaggg tgctggagct
    67501 tattaaaaga agaaaaatca atgcccagac ggtaagaacc atagtcattg atgagggaga
    67561 cagaatgctt gacgagcaca atttgggcac tgtcagggag gttataaaaa ccaccttacg
    67621 ggacaggcag cttatggtct tttccgccac cataggtgaa aaagccttga gtaccgcaag
    67681 ggaattcatg aaggatcccc aggtagtccg aattgaagac agaggaaaaa taagtccgga
    67741 tatccggcat atatacctgg cagctgagaa aagggataag attgaagtac ttcgtaaact
    67801 ggcggcatca atgaagcctg aaagagccat agtatttatt aataaaagcg aggagctgca
    67861 gctgacggct gcaaagctta aatatcatca tctcagcgct tgtgatatat ccggcagctc
    67921 ttcaaaggaa gagcggaaaa aagcattgga gggcttcagg aagggtgata taaagctatt
    67981 ggttgcctcc gatatagcag ccagagggct ggatataaaa ggggtaacac atatattcaa
    68041 tctggacctg ccggaagacc ccaaaggata tttgcacagg gcaggaagga caggaaggat
    68101 gggggaaccc ggcacggtag tctctattgt tacagaaagg gaggttcctt tcttaagaaa
    68161 atgtgaaagg gatctcaaaa taaaaatata tgaaaagata atcgccaggg gaaaagtgct
    68221 ggatcctccc caaagtaaaa cgtcagcagg aaaaggggcc gggagataag ctgatatacc
    68281 gcatatagct tcccggagag gcgttatata tttggaataa ataaaccaaa tatgaatatt
    68341 catgatataa ttacttttta atcattcatt gtattataat aataaatgtt attaatgagt
    68401 gattaatgaa atgcacatat ggaggaggca gcagtattaa aaatcttacg agtagaagtg
    68461 catagggatg ggtactttat agatcgaaaa tttgtggggt tggctgacat accaggcatt
    68521 aaatctattt atatggtgat gaatagaaat caaaaatgag cggtcaatgt ttgtactctg
    68581 ctagtattat tgagatagga gggattacgt tgataaaaat tggcataata gttcctaacg
    68641 atttagtaaa tattgcaaaa tcagctgcag agggaattga agatgaaata acgattttat
    68701 atggttcaat gactgaagga attagtttag ctaaacaact ggaagagtgc aactatgata
    68761 ttattattac gcgtggcggg acacaagttt tattaaccca gtctaatatt aatataccag
    68821 ttatatcaat tccaataact ccgatagatg tttatgaagc tgttaatgag gctgaaaaga
    68881 ttagtaaaga tatatcgttt attgtatctc aaaatatgat ttcgataatc gaaagttaca
    68941 taaaaataag tggtagaaat tttaaggtat ttcaaattaa agaagaatca gaaattgaaa
    69001 aaaaaataag ggaacttgcc acagaaggga aaaaggtagt agttggctta ggtacgatag
    69061 ctaaatatgc tagttcatat ggtttggtac ctatagtgat taaatcgggc aaggaatctt
    69121 ttttatctac tattatggaa gcaaagagaa ttgttaatgc tacaaaaaaa gaaaagaaag
    69181 ataaagaaag aataaaagcc attattgagc atactattga aggtataata tgcgtagaca
    69241 agaaaggtca tataattatt atcaatgaag ttgcacggaa attacttaaa tgtgataata
    69301 atgggttagt aggtaagatg ataagttcgg ttttaccgga tctccaactt gaagatactt
    69361 tatataatgg tataatggag acggaagtca ttaaaaattt aaaagacact aaagttatgg
    69421 tttataaaat acctataatt gtcgatgacg agatagaaaa tgcggtagct gttttgagag
    69481 atatagatga aatacaaaag gcagatgaaa aaatacggca ggacatagct gtaagcggtc
    69541 attttgccaa atatactttt aacgatgtaa tagggtgcag cgaatctata agagaaatag
    69601 tcagaatagg aaaagagtat gcaaaggtta actctactgt tttaatggaa ggggaaaccg
    69661 gaacgggtaa agaaattatt gcacagagca tacataatta cagcaataga tctaataagc
    69721 cgtttgttgc tgttaactgt gcagcactat cggaaagctt gttggaatct gaattatttg
    69781 gatattcctc cggtgctttt acaggagctg atcgtaaagg taaaagaggc ttgtttgaac
    69841 aggctcatac gggtactata tttctggatg aaatatctga gataaaccca ttattacaag
    69901 gaagattgct tagggttctt caagagaagc aagtaatgag gataggagat aataagctta
    69961 ttcctgttga tgtgagagtt atttcagcca cgaataaaga tttgcataac cttgttgttg
    70021 ccaaaaaatt tagagatgac ctatactaca ggctcaatgt cctaagaata gaattaatac
    70081 ctttaaggta cagaagggaa gacatacctt gtctgataga ttattttcta agagcttatt
    70141 gtgcaaaatt aggcaaagag aatattgttt ttacttccga agcagcagaa catttatcaa
    70201 agtatgactg gccaggtaat actcgtgaag tcagaaattt ttgtgaacga ttggctgtaa
    70261 tggcaaaaaa aacaataatt acattagagg atgtaaccaa tcatataata aatattgggg
    70321 ttaattacaa taatgcagat gaacttgttg atattgtcgg aaaaaataaa aacctatcaa
    70381 aagacaactt aaatcttaat aacatgacaa agcaaagtat tgaacttgct ttaatacatt
    70441 ctaatggaag tataactaat gctgcaaaag aattaggagt ttcaagaact accatatgga
    70501 gaaagatgaa aaagtataac ttattggtta aaaaatgaaa cacggctact taaagcgttg
    70561 cataatgaaa cacatttgaa acataaagaa tttaacatgc agtagcaatg ctgatattct
    70621 aataatggaa gctatgcctt actagttggc atagcttttg ctattaatta gtgtaaaaga
    70681 aattagtgcg aaaatcaata tagaaagaat tacattatta agggaggaaa ttttatggaa
    70741 agaagaacat acttacagat tcctgggcca acaaatattc ccgacaggat attgaggagc
    70801 ttatctcagc cattgataaa tcatagaggt ccggagtttg gagaattact gacaaaatgc
    70861 gttgaaggtc ttaagaaggt ttataaaaca aaaaatgata tactaatatt tccgagttca
    70921 ggtagtggcg taatagaatc cgcaatagta aatttgtttt ctcccgggga tacaatatta
    70981 acagcttcca taggggtatt tagtgaaaga gcagctacga tagctgaaaa attcgggttg
    71041 aatgttatta ggataacaaa ggaatgggga gagtcagtca aacctgaaga tgttaagaaa
    71101 gtattagaag aggatacaga caaaaagatc aaaggagttt ttgtaccaca caatgaaact
    71161 actgctgctg ttgcaagtga tataaaagga atttcagaag ttataaaaga aatcaaccat
    71221 ccagctgttc taattattga tgcagtaagc tctttagcat gtttgcctat tgaaacagat
    71281 gattgggcaa tagatattgt actgactggc tctcaaaaag gtttaatgct gcccccggga
    71341 atgggaattg tatcaattag tcctaaagct tgggaattgg tagaaaagtc aacattacca
    71401 aaatggtact ggagttataa ggctgttaaa gaaagaatgg agattaaaga atttccttat
    71461 acaccggcga ctacattatt attcggctta agtgaagccc tcgatattat cgaagaagaa
    71521 ggattagaaa acgtaattgc aagacatgaa ctattggcca cagcagtaag agaagcagct
    71581 aaagctatgg ggttaagtat atttactgag gaaggatatg agtctaattc cgttaccgca
    71641 attattatgc cagaaggtat tagttacaaa gatttagctg gtatattaaa aactaaattc
    71701 ggcgttgttg ttggaggagg tttacagaag cttaaaggaa aaatattaag aatcggacat
    71761 ttaggttcta tccataaggc agatatttat gcaataatgt gtgctgtaga agcggcattg
    71821 tttgaattgg gatataaggt agaacttgga tcggcagcta gagcagtttc aaaagttttt
    71881 ttaaatgata agtaagataa atctttagct taaagcagaa atgcaataat tgcttgaata
    71941 ttacaaaatg gaggtattta aatgtattat actactaagg aaattgaaca caaatttgtt
    72001 acagatgttt ttatgcatct tggagttaag gaagaacatg ctaagatggc cgcagatgta
    72061 gtagtacagt cagatattac tggtgtaact actcacggat tggctcggat gccgatgtac
    72121 gtaagaagat tattacaaaa aacagttaac ccaactccaa atattagaca aacaaatgat
    72181 tctgagaatc taatagcttt agatggagat aatggtccgg gaaatgtagt aggaccaaca
    72241 gctataaata tgtgtataga ggcagcaaaa aaacacggga ttgcagctgt atcaatcaaa
    72301 tatagtaacc actacggtgt tgggaattat tatgcatgga ggtttgctga agcaaatctt
    72361 ataggcatta ctatgacaaa tagtactccc agtatggctc caaccggagg taagacacct
    72421 atgctgggaa caaatccaat aactatagca gttccggcag gaaaacgtta tccaatatgt
    72481 ttagatatgg ctaccagtgt tgtagcaaaa gggaaattat taaaagcagt tgaggcaaat
    72541 agtaaaattc catttgaatg ggcagttgat aaagagggaa gacctacaga tgatccgaat
    72601 gaagcattgg agggctcatt attagcaatt ggtggatata aaggatacgg tttagctatg
    72661 attatagaaa ttctttcagc agttatatcc caatcagctt atggattaga tattggagat
    72721 aaatttgatg ctgattgtac agaccaagaa aaaataggtc actttatgct tgctatagat
    72781 gtttccaaat ttttggattt agatacattt aagaaatcta tggatcaata tatagaaaca
    72841 atgaagaata ctgaaaaggc tgccggagta gaagaaatat atgtgccggg tgaattggaa
    72901 ttcttaagag ctgaaaaaat aaaagagacg ggaattcctg ttccggaagg aatacaagca
    72961 aagatgctaa aaattgcaaa agatgtagga ataggatctg atattaagac tatagatgat
    73021 ttgtttgaca gatataaata ataaaattga agagctgtag gtgaaactct acagctctca
    73081 ttccattttt tacaaaaagt gcgaggtgcc ttaatgaaac agtatattga tttacggtca
    73141 gatacagtta ctcaaccaac ccaagagatg agagatgcca tatataatgc tatagtaggg
    73201 gatgacatac tgggtgaaga tcctacagta aaggaattag aagagctggc agcaaaaatg
    73261 ttcaataaag aggaaggact ttttgttagc tcgggaacta tggctaacca actagcagtt
    73321 atgactttta ctaatagagg agaagaagtc attgttggag acacatccca tatatacaat
    73381 ttggaagtgg gtggcctatc agccttgtca caagttcaaa ccagaccaat acatgttccg
    73441 gatggcattt atgatataga tattctggaa gaattaatac agccaaaggg gatacaatca
    73501 ccgaaaactg gattaatatg catagaaaat actaataatt tgaatgctgg tttagtagta
    73561 ccagtagata atattaatga agtatgcaat ttagctaaaa aaaaggatat ccctgtttat
    73621 atggatggtg caaggatatt taatgctgca atagcgttag atatgaatgt aaaagaaatt
    73681 gttaagaata cggatgcggt tatgtttgca ttatcaaagg gtctggctgc tccttttgga
    73741 gctgtgctgg taggaagcaa ggagtttatt aataaagcaa ggtggttcaa gcagcgcatt
    73801 ggcgggggtt atagacaggc aggatttatg gccgcaccgg gagtattggc attaagaact
    73861 atgatatcgc gaatttccat agatcacgat aatgctaaat tactaggtaa tggcttatca
    73921 aatatagttg gtctaagaat agatacaaca cgaatacata ctaatatcgt ttccgggtcc
    73981 attgaattgt tgaatattac aattgaggac ttcttaaata ggttattgag caaaggaata
    74041 aaagctaaaa ggatttcaga aacatccttt aggatggtaa cacatttggg tattgatgaa
    74101 tctcatatta attatgttat agaatctgta aaagatataa ttactgattt atgattattc
    74161 attgtagccg tttacataat aatcatgata ttgtccaaaa aataatgagg aggatgctac
    74221 gggcaatgta gaataacatt ataatgaaca tgtcatttta tcttattgac ataataattt
    74281 atatagaata aaatacttat aatacttttt atccatgaac gatacaaatt accggattaa
    74341 tactaatgtt tgatgacatt gttagttaga tttaacaaat agaattatta cctatcatgc
    74401 agccctgcct agtacattag gaaaatcggg gggaggggta aaatgagtga ggatactata
    74461 ctaataggag ggtattcgaa ctttcccagc ggagcagctg caaaggaagt ctatgggatg
    74521 ttaggtgtag cgcttgagat tgacccaaag acgggaaaaa tagtaaacgc tgctactact
    74581 cttctgacag atgtgacaaa gaattttgtg agccgaatat tagtaggaca aaacatgaac
    74641 gaaggtttaa aagagccaat taaatcaatc gaaagaagat acttcgggag aaataagaaa
    74701 gcattaatag ctgcattaat ggatgcacac aggatctacg aaaaatacct ggaggaaaag
    74761 tataatcttt aaaaaaggaa tagtgaatga ccgcagggct gctgtgttat ttattgtaga
    74821 gttaacaact ctatcaaaca taaataagta ttcaggaggt tatgaatact tattttttat
    74881 ttttaatagt ctgaatattc atcctattaa aaaatcagaa cgcagtgctg cccgaatact
    74941 gaaaatcgaa gcaatgattt taagcctaaa gggggtgagc ctatattaca atgggtcatt
    75001 cgctaaagta tgattcattt ttacaataca aggagggtga gaaagtggaa aagatgtatg
    75061 ctgaaaggca caacaagctg ctggaggtaa tgagagacaa aggatgggac tgtgtgattt
    75121 tatggccgtc gccaaacatg tattatatga cgggttatta tccaaaacca gatgagcgat
    75181 tgcagatagc ttttatccct gtgcagggag agccggttct tatagttccg agattatact
    75241 ctggcgaatc caagcataaa tgctggataa aggatcaaag aagctggata gatggcgacg
    75301 atatacttgc tttaaccaag gatgtaataa aagaccttgg tttaaaagac gcacgcatag
    75361 ccctggatga tactatgggt tttttgcagg tatcaaccat tcaagcagcg tgtccaaaag
    75421 ctgaattcgg tttagccggt acagtgatgt ctccgctcag gcttgaaaag ggtccggagg
    75481 aaatcgcgtt aatgcaaaaa tccagtcaaa taagcgatga ggtaatgcaa ataggtatcg
    75541 atgcatgttt gagcggacaa aacgaacagc agctagttga cttagtggag tatgaattaa
    75601 ggaaaagggg tatggccaac ggtttcagca acctgattgc ctcaggtact cacagtgctc
    75661 ttccgcacca tgtttcgagc aaaagagttc ctcaaaaggg cgatgcggta tttcttgaca
    75721 taggcggtgc ctatgaaaga tattggtcgg atataacaag gactattcat ataggaccac
    75781 catccgctaa atttattgaa acgtacgaag cggttaaaga agcccagcag agggcaagtg
    75841 aggcagtaaa gcccggagtt actgcggaag aagtgcatga aacagccttt ggatacctgg
    75901 aaaagaaggg tcttagcaag tatttcttcc acagggtcgg tcatggaatg ggtctggaag
    75961 gtcatgagct catttcgatt aataggggaa accaccttgt attgaaacca ggaatgactt
    76021 tttctgttga gccgggagta tacttcgagg gtgaattcgg tatcagaatt gaagatactg
    76081 tagtagtgac cgaaaccggt cataaatcgt taactgagtt tacgcgtgac ctgattgttc
    76141 tgtaagcatt gtttgtttat tgacattaga tatatatctg atagataaat aaaaggagga
    76201 gaaagtaaat gaagagaatg cgttatcttg cactggttat ggttcttgtt atgctgttga
    76261 gcgtaggttg tagctcaagc ggcggagagg ccccgggggc agaggcacca agtgaagcag
    76321 gaagcgaggc tagtactcca gcgaaaacca cattggtata tggagttggt gtggatgcaa
    76381 caaccatgga cccgcatttt gcgaacaaca ctccaaccgg aaatgtatca ttccaggtac
    76441 acgagaattt gacagctttt gatgatccaa aaacaatgaa accggtgggt aaactggcaa
    76501 aggattggaa aatttctgag gatgggctta catggacatt caatttaagg gaagggataa
    76561 ccttccatga tggagcgcca tttaatgcgg aagcggtaaa gaaaaatttt gaccggatac
    76621 tgaatccgga tttaggtgct ccgcgccgat caaacttcaa aatgatagac agactggaag
    76681 ttgtagatga gtatactgta aacataataa ccaatgtccc ctatgctgcg cttcctcaac
    76741 ttcttacctg ctataacgca agcatattga gccctaaagc aagtgatgag tatggagatc
    76801 aatactctgc tcatgctgca ggaacaggtc cctttaagtt gggtgaatgg atacccggag
    76861 aatcaatgac actggatagg aatgacaatt attggggtga aaagcccagt ttagaaaaat
    76921 tagtgtttaa aataataccg gaggatgcca gccgtgtaat ggcggtacag accggagaag
    76981 tagattatat ttccaatgta cctccctttg aaattgaaaa cttgaaatct agtggggatg
    77041 ttacagttga gatgcacgaa ggtttccgca caatatatct tggaatggtc attaacagac
    77101 ctccctttga taacatcaag gttagacaag cattgaacta tgcaatcgac cgtgatgcga
    77161 ttataaacga tttcttggag ggctctgcta cttatgcaat aggtcccgaa gcaacctcaa
    77221 taccgggggc atcaagtgat ctctttaagt atacctatga cccggaaaag gcaaaggctc
    77281 ttctggctga ggcagggtat cccgatggat ttgaaataag atttgtaagt ccccatggtc
    77341 gttatataat ggacagacag atagcagaag cgattcaggc acagcttagc aaggtaggaa
    77401 taaaagcgaa tctgcagata ctcgactttt cgctgctgct taacatgctg cctacaggaa
    77461 aggacgcgga attaatgttg atgggtaaag gctcacctgc ctccgatccg gattttgact
    77521 taacaaatta ttttactacc gatgggacaa acaactggta ttttatatcg gacccgaaaa
    77581 tagatgagct cactgaaaaa cagcgggcaa cgatggactt aaacgagcgc tatgaggttt
    77641 tacaagaact gcaggaaaag gtacaggaag aactgccttg ggcatcactc cactatgaga
    77701 gacagatagt agcgttgggt aagggcgtag ccggagtgac agtatggcca tatgagtata
    77761 ttgactttag taaggcgaaa ttcgaataaa tcacataaac aagaatagtt gtttgacatt
    77821 gggaggctac tgcctcccaa tgacaaactc cattttatag gtggtggaaa atttgtttaa
    77881 atacttaaaa cggcgcttat taatgttaat tccattgctt ctggtggtat ctatcatagt
    77941 tttttcaatg atccacctga tgcccggtga ccctgctatg ataattgcag gcgagggtgc
    78001 aagccaggca gatatagaag cggtaaaaca caagcacggc ctagacaagc cgttatttgt
    78061 acagtacttt acatggttgg gaaatattat acgcggtgat ttcggtaaat cattacgtac
    78121 cgggcgtcct atatctcagg agattggctt tcgttttgta aacacaataa aacttgctac
    78181 tttagcaata gttataggaa gcatactcgg tctagtgatg gggattgtat ctgctaccaa
    78241 gagatattca ttttttgata atgttacaat ggtaattgca atggttggtg tatctattac
    78301 tccattctat ttaggactga tgtcaatatt attgttttca gtaaaattgg gctggttacc
    78361 aatgatggga gttgaatcct ggaaaggttg gatacttccg gccactactt tggcgtttag
    78421 gcctgcagcc atgatagccc gtatgacccg ttccaatatg ctcgagattt tgggtcagga
    78481 ttatatattg tcagctcgcg ccttaggatt acccaataaa gtagtttttg taaaatatgc
    78541 attaaggaat gctttaaaca ctgttgttac agtaatcggt ttgcagcttg gcttgttaat
    78601 gggcggtgcc gtgatgacag agactgtatt cagcctgccc ggcataggac gccttgcagt
    78661 tgaaagcata aaaggaaggg attttccgtc catccaggtt tcggttctat taattgcatt
    78721 gagctttgtt attataaacc ttgcagttga tatactgtat gccataataa acccaagaat
    78781 tagttacaac tagtcgctga agggagggga acactttgga atcaaataat aatactgcct
    78841 caatgccaat gaacgatgcg aaaatggaga gagaggccag aataagagaa ttaaaacgtt
    78901 tctggttaca atttagtaag cgaccggtag cagtactggg tttagtgatt gttttaattt
    78961 ttttagtaat gtgtataact gcgccgctga tagctccctt tgacccatat gaatcagata
    79021 ccagcaaaag attgctgaaa ccgggagaag aaggtcatat cctgggaacc gatgagttag
    79081 gaagggacct tttcagccgg ataatattcg gctctcggta ttcattgctg ataggggtag
    79141 gttcggtagg tctgggagct ttgataggaa ttcctattgg gcttattgca gggtattatg
    79201 aaggtaaaat tgggaatgtt ctaatgagga ttatggatgt atttttggca tttcctaata
    79261 tattattagc tcttattatt gtaagtatcc tgggaccaag tttatacaac gcactgatag
    79321 ctttaagtat atggtcaatt ccttcttttg cccgtatagc aagaagcgaa gtattagcgc
    79381 taaagaataa tgaattcgtt gaggcttcca aggctatggg ttcaagagac agttggatag
    79441 tactatggca catatttcca aaccttgtgt cccagctgat agtatggatt actctaaata
    79501 taagcagtgc aatactgaca ggtgccggat tgggatttct cggtcttggt gcacagccgc
    79561 catctcctga atggggagtt atgctggcaa acggcaggca gtatataatg agggcacatc
    79621 atttaactac atatcccggt ctggcgattt tgttccttgc acttgggtta aatcttctgg
    79681 gcgacgggct gagggataca ttggatccaa ggttgaagga ttaagtatac ggaatgaggt
    79741 gacaaagtgc atactacttt aaaaataaaa gatctgtcag tggaatacca taccttcgac
    79801 ggtattgtaa aggcagttaa taatttaaat ctagaagtga agaagggcga aagcattggt
    79861 ttggtcggag aaacaggagc agggaaaaca acaaccgccc tatccatact taagctattg
    79921 ccttatgcgg gtcatgtagt aaatggggaa attgaattta atggtaaatc tttattaaag
    79981 gcatcagaac aagagatgta cagaatacgg ggaaataaaa tttcgatggt tttccaggac
    80041 cccttaactt ccctaaatcc ggtttataat attggaaccc agatacgtga tgttttgacg
    80101 catcaccaat cgctgaacaa ggaagaggca atcgaaaagg cgaaagagat gatagaattg
    80161 gttggaatac cgtcaaacag gctaaatgaa tatccccatc aatttagtgg aggtatgaga
    80221 cagcgtgtag gtattgccct tgctttagcc tgcaatcctg agctattgat tgccgatgag
    80281 cccacaacag cattggacgt tactatccaa gcccaaattc ttgctcttat gacagaactt
    80341 aagcaaaagt tccagatgtc acttatcatg ataacccata acctaggaat agtggcagaa
    80401 gtctgtgaca gcgttgctgt tatgtactcg ggagaaatta tagagatcgg gtctgtgaaa
    80461 aaggttttta acaatcctat gcattactat acaaagggac tttttggatc tgttccgagc
    80521 cctgatgtca aaatcagcag actcaatcct atcaagggga gaatgccgga tcccaaaaac
    80581 ctgcccaaag gatgtaagtt ctatccacgc tgcaacgctg caaggccgga atgcttgaaa
    80641 atgcctgaac taattaaggt agaagaggat cattttgtag catgtgttgc aggcaaggag
    80701 ggagtatagt gagcaaaacg tttattgaga taaaaaattt aaagaaatac ttcaatgtca
    80761 aggacgggaa aaaattacat gctgtagatg atgtttcttt caacgtagac aagggtgaaa
    80821 tagtaggatt ggtaggagaa agcggctgcg gtaaatctac caccggcagg gttattctaa
    80881 ggcttttaga ggctactgca ggtaatgtat tcttggaagg ggaagatatt attcaggcta
    80941 acaagaatag actgaaacag ctgcgcagga gaatgcagat agtgttccag gacccgaatt
    81001 cttcactaaa cccaagaaaa tcggtgcgtc agattatttc ggaaccgcta atagttcaag
    81061 gaatattaaa gagcaaagag atgcaggaaa aggtatatac ccttatggaa gagaccgggg
    81121 tggcgtttga tttagtgggc aaatatcccc acgagctgga tggtggggtt aggcagcgtg
    81181 taggaatagc aagggccttg gctcttggtc cggaatttat tgtgtgtgat gagcctgttt
    81241 cagccctaga cgtatcgata caagcccaga tgttaaatct ttttcaggat cttaaagaaa
    81301 agaaggacct gacatacctg tttatttccc acgatttaag cgttgtcaga catatctccg
    81361 accgtatact ggttatgtac gtaggtcggc ttgttgaaga ggcagaagca gacgatttgt
    81421 ttataaaccc gatgcatccc tacactaagg ctttgctgtc ggcaatacct gtacccaaga
    81481 tacgcaagga gagtaatagg attcttttga aaggcgacgt tccaagccca atagatcccc
    81541 caccgggatg caggtttgca aaaagatgct ggatggcaaa ggaagaatgc tttgaaaaag
    81601 aacctgagct aaaagaatat agcccgggtc atagagcagc atgccacttc gttgagtaaa
    81661 actataccgc tgaaaggagc ataattatga cagagaataa agcttatatt ttaccggaaa
    81721 tgacttgggt tgaagcggaa aaggcctata aagaggccaa gattgccata atacccgttg
    81781 gctcccacga acagcacggg ccgggcataa agatgaaggt agacggtgtt caggcttatg
    81841 aactggcaaa actcgttgct cagggagtac acccgcttgc ggtggttact ccgccggtaa
    81901 atatcggtat ttcccatcat catatgaaat accccggaac agtaacgctg aagagttcaa
    81961 catttatggc agtaattgaa gatatagtta caagcctata taggtttggt ttcaaaaagt
    82021 ttcttatcat aaacgcacat ggcgggaacc agtatgcttg caaccttgct acaaatgacc
    82081 ttaaagaaaa gttgggagta gaaattgcct atacacttta cctggatttg gtgccggaat
    82141 tggtggaaag ggtacctccg gaaaagcgcg gacatggcgc agacccgggt ttggcaccgg
    82201 ctctgtacct ggatgaggac ctagtgtata tggatagact cgaaaagggt aaagaacact
    82261 ggccttataa aatgatagga cgggaaaagg ttaaagttaa tttcccctat tatactcatg
    82321 aaatcagtga taatggagta tggggagatc cggaaggcac cagcaaagaa ttcgggaaag
    82381 aaatatgcga tgctgtagta gagcgcctag gtgtatttat aagggatttt gtaaaaaacg
    82441 cataatatga ggtgatataa tggtaaactc aaccaaggaa atgaaggttg caatacatga
    82501 ccttacttgg ccggaagttg ataaaatgag agatcaagta aaaactgtaa taattccggt
    82561 aggctcaaca gagcaacatg gccccaacct ctgtatatct accgatgcaa cacgggctta
    82621 taagttttct cttcgtgttg ccaaggcttt gtatcctcac gtattagttg caccgtgcat
    82681 tcatgtcgga atctccggcc atcatatgga ttttcccgga actattacat taaggccgga
    82741 aacaatgatt agcacaataa ccgattatgt gagcagtttg atgaagcacg ggttcaaaaa
    82801 gttcttcatt attaatgccc acgggggtaa tgaaggtact cttcaggtgg ccgttgaaaa
    82861 catcagaaca gattttggta taagaatacc cttcgttaat tataaggttc tttgtcaaga
    82921 tgttaccgca gagattgtgg gaacaaaaag agtagaccat tcgggtgaat ttgaagtatc
    82981 tgatgctatg tttttggctc cggaaattgt aagagaagat gccatatgta aaggagacga
    83041 tgggaacttc cctttagtat atactgatat atatggtaaa tacagaataa attatccgtt
    83101 gaattggaaa caggtagctc caagcggagc aatgggtgag gctcataaag cgactccgga
    83161 aaaagggaaa cagcttaatg atgctgcact taaaagggct gttgagttct tagagcagtt
    83221 tatggaagat gaagattagt gttgagaaag ttggcaaaaa cgtgccgtgt catgttggct
    83281 tggctattga ccatcattaa tcggataagg aggtatatca atgtatatta accaagaact
    83341 ttgtgttcaa tgccatgcct gtattcctta ttgtccaatg aaagcaattg aatacaagga
    83401 agaaagcaag cgtgtagaga taaatataga cgagtgcgtg gaatgtgaaa tctgcggtaa
    83461 tgcagacgtg tgtcccacag gtgcactgac catggaagag atgaaatggc cccgtatatt
    83521 aagaaatcag ttttctaatc cgcttattac ccaccctgat accggggtgc cgggacgtgg
    83581 caccgaagaa atgaagacca acgacgttac gggcaggttt aaaaggggta tggtgggaat
    83641 agctattgaa atgggacgcc cgggaaccgg aacacgtttt tatgatgtgg aaaaagtagc
    83701 tatggctgtt gcaaaggtag gagtacattt tgaaccccat aatccggtga caaagctgat
    83761 gacggataat accaccggaa aaatgaagga cgatgttcta aacgaaaagg ccttgtccgc
    83821 aattgttgaa tttgacattc cgattgagaa aaccaaaacg gtattggaac agttgaaaac
    83881 tgtaacgcct ttaattaata ctgtgtttag ccttgacctt gcttgtcgtc ttaatgaaga
    83941 cggcagcaac cctgcagttt gcctggctga agaagcaggt tataaagtat ctgtaaacgg
    84001 gaaaacaaac gtcggtttag gcagaccttt ggctaaggag gaataaaaat gacacatact
    84061 ttacatcgca aaggagataa agaaggttta tccaatgatt atatagtatt cgctatctct
    84121 gctcaaactg ttaatgcaaa aggcagcgca cctaaattta aggagtttgg agacattgtg
    84181 ttaaaatata atccggtaaa ctacggggac atgaagacgg gaaatgcgta caatgtaggt
    84241 gttgacaaag tacatgaaag ctataaggat aactctattg tacatgcggt gttcacagaa
    84301 gaagacattg ttgctaaagt gcttaaggaa cttagggaag cggacttagg actttcaatt
    84361 gtgctatcgg gaattgttga acacacatcc gaatgctgtc acaaaattag tattgaacct
    84421 catactattg agtattccct tggtatacat ggaaagacag acagtctccc ggaagataat
    84481 gtagttgaga ttagtactat gtgtggtcat ggaatggtgt ccttttcgct gattgaacac
    84541 ttttgtgaag agatagaaaa gggtaggatt actgcagatg aggtggcaag aaagctggct
    84601 aagcagtgtc actgcggagt gtttaatcca acgagggcgg cagagatact acgattaatg
    84661 actgatatgt aagatttgtg atatcagtaa gggcagaaga taaggaatct tctgccctta
    84721 ttgttaagtt tagacattta ataagtaaag aaacagaatg gcgtataata actttgccta
    84781 ttgcaaaata gagtccaaaa aagacgagaa atatagttat aaatgtgtta tatataatta
    84841 tattggtgaa ctttgagttt gaaatgtacg ttcaaattag ctattgctaa gattatttga
    84901 aggagagaat actatgacca tatatggagc ggcaattatt gccggatgta tgttcgttgg
    84961 aagctttata ggtgatgctc tggggggatt gatgggcata gatgccaatg taggaggcgt
    85021 tggattttcc atgctttttc taattctaat taccggttct gatttatttg ggaaaaaatc
    85081 gtcggcagca acttccgaag ggctggaata ctggcagagc atgtacattc cggtggtagc
    85141 ggcaatgact gcatcacaaa acgttgtaca ggctgtgtca ggtggggtta tggctgtcct
    85201 agcagggctt attgcagttg ctgtaggatt tgcacttctg ccggtattga ataatatgtt
    85261 taagtcatac gaagtggagc gggaaacgga ggttaaagga gaatgaacgc ggtttttgag
    85321 gttgcccaaa atattatgat caataacggg cttgtcatgt cattcctttt tataggcctg
    85381 ttaatgtggt tttctactat aatttcaaaa tatctgacta aaggaagaat acatagttcg
    85441 gctatagcca taattctagg gcttgttttg gcttatgtgg gtggtgtatt gaccggggga
    85501 aacaagggaa ttgcagatat tgcagccttt actggcgtag gcgtccttgg aagctcaatg
    85561 ctgcgtgact ttactattgt atcaactgca tatggtgcct cgatttccga aataaaaaaa
    85621 tgtggcattg tcggtattat atcactatta ttgggggtag tgtctgcttt cctgataggg
    85681 gcaatttttg ccgtttgctt tggttatgca gatgcggtaa gtattactac aattgcagcc
    85741 ggaacggtta catttgtggt ggggccggtg accggttcgg ctttaggtgc tagttctccg
    85801 gtgatcacta tttcaatagc tgccggagtg gtaaaatcta ttgcggttat ggtggtaaca
    85861 ccatttgcag caaagagaat tgggttgact acaccgaaag ctgctatgat atacggcggt
    85921 ttgatgggca ctaccagtgg agtagcggcg ggattggcgg caaccgatac taagcttgtc
    85981 ccctatggcg caatgacagc aacgttctat accggtcttg ggtgtttgct ttgccctact
    86041 gtactttttt ggttaacgcg gcttatgttt taaaaggata tccctgttta tatggatggt
    86101 gcaaggatat ttaatgatgc agtagcgtta aataagacga tgcatatcca cttattcctg
    86161 ctccacaact cttaattcaa tactgctttc ataatgttaa taaatttcct tgcaggcttg
    86221 gaaaggtaat gattcttttt ataggcggct attaggttac gagaggaatt gacattgtct
    86281 aaataataat atataggatg attcgctaga tttccgtatt tgacaaagct gtgaggagta
    86341 aatgacactc ctaatcctga aagtgtcaaa ttaaaaattg tgtgaatatt ggctgtaatt
    86401 aaaaatggat ttggttcaaa ttttgatagc ctgcaaagtt caactgattt tttgtatagg
    86461 tgctgggaag tgttgacaag taaaaaaggt tcatctttaa aataactcag aagttctata
    86521 ggcatttttt tagaatcaaa attagcattt acaatatcat catacgaaag ttgatagctt
    86581 tttagcttgt tgtttattgg attagatttt gataccgtta ggagtattat ttcacgagct
    86641 attactttat aattgaaaat atttttattc agataagatt ctgttgaaac gaaaatatcg
    86701 attgatactt cttgtgcata atttgataat tcctgcattg ttcctgttat cagatgaaat
    86761 tttacttttg gcatactggt tgtaaaggct tttaaaatgc cgggcaatat ggtagaacaa
    86821 cgataaggag tcattccgac tgtaagagaa ctgtttttaa aatcagcgat atctgaagtt
    86881 ttattttcca gatcgtattg cagtgttcta attttatatg cagtatcaat aaatatacga
    86941 cctgcttcag ttatttcaat aggatttgta ctgcgattaa acaatgaaac atccaattct
    87001 ttttcaactt ttgttatgca ttggcttaaa gagggctgtg aaatacaaag ctttttggca
    87061 gctttggtga aattcctttc ttcagccaaa gccaaaacgt aatcgtagta gtaatttttc
    87121 atatctactc acattccttc ctcttaatac tcaatatttt tagtgtaatt ttactcgctt
    87181 gttgcttagt acctataaat cgcctataag aaccatgcag aattcattcc ggttatgctc
    87241 gatcttcatt ttgtttataa tgtgatatag gttttaccta tgatagattg agttattata
    87301 ttagacaata gcattatata gtgttataaa aattataggg catatttatt aacaaatcaa
    87361 ttatttcagt agctgaatct ttaaacatta aattagtaat ggaagtgtta cttattagag
    87421 agaaagactg gaaatttctt tcataattct gtcattattt gaaagtaaag acttccgact
    87481 attggcatat taaaaaataa ggggattaat agttcaccat attaaaaaat tacgaggagg
    87541 aagtaaaatg aaaaagctag tatctttatt aatggtagtg atactcatag tagcactgtc
    87601 aggatgttcc ggcactacag gaggtccggc tgaagaagca aaggaacctg ctgaaaaaag
    87661 cagtaaatac ccttcaaaag ctattgagat tgttgtccct tatgccgcag gtggtggagt
    87721 agacatgaat acacgtgcta ttcagccttt cctggaaaaa gaacttgata caacggtggt
    87781 cgtaatgaac aaacccggcg gaggcggcgt tctaggcgta acatacgcca ttatgtcaga
    87841 accggatggg aataggttgc tccttactaa cctaattgct atgtgttctg ccagtgcact
    87901 gaatacgctg caagtagact tactagaagg tgtggaatac atagggggta ctgtagttga
    87961 ccccggagtt atagcagttt caaaaaacag taaattcaat agtttagacg aaatcattga
    88021 agaactaaaa aagaatcccg gcagtttatc ttttggtgta acaggagaaa ctagtctgga
    88081 caatttaata tgtaagaacc ttgaggttat aacaggtgca aagtttaata cggtttcatt
    88141 tgatggcggt tcagaggcta tggctgccct tctaggcgga catattgata tcatgggggg
    88201 aacactttct gaaatgctga cttatcataa atccggcgaa gtgaagtcat tggcagtagg
    88261 tcatggagag agaatcgatt caatatctga tgttcccaca tttgatgaat taggttacaa
    88321 aatggctgta actgcagcaa aacgtgccat aatgatgccg gcaggtgcag atgaagagat
    88381 tgtaaaggtt ttgcaagatg ccgttaaagc agctgcttcc agtgaggaat ttgcgaaaga
    88441 atgcgaatct ttaggtttga tggctagtta ttttacacct gaagaatgtg ttaagacagt
    88501 aaccgaagta tctgagttcc ttaatggatt gtatggaaaa aaatagagtt tgtgtgaata
    88561 gaatacacaa atagaatttg tcatttaatt tactgtgttg attcttaata gttgattctc
    88621 aacgatgaaa gaatcaacac agtaaaaaaa gtatagtttt gattcgagcc atattttctg
    88681 atccgttaaa gtcagcaata attataatta ttaaggcata taaaagtata tttaagaatc
    88741 attaatggct tgactaacaa ataagttttg gaggtataat tccccttgcg cattcagaat
    88801 atggagatgt tacaaaatca tgggaataag gttggcaggg ttcatgttgc acagatgctt
    88861 gaagccggga tgaacatggt ggattcttat atcggagcta gcaggctaat atcccgtaag
    88921 gataactggc ttattttcga tggaaaagaa tttgagatgg acaatgaccc gcgaagtgga
    88981 actgctaagt atgatctgga caaatttgac agagtaattg taattggcgc agccaaagga
    89041 gttcaaagat gtgcgatatt gttggaagag gttctgggag aatacctaac cggcgggcat
    89101 gtcataggta aacacggtga tgatattata tgcaaaaaaa taggtgtcac cctggcaggt
    89161 caccctattc cggaccagat gtgtgtagaa ggctgtaaaa agattcacga atggattaag
    89221 gatataaacg aaaaagatct tattattaca attgcaggta gcggagtatc ttcattaatg
    89281 acgtggccga tcgcaggatt cactattgat gatcttcgaa acctgaccca tatgatgcaa
    89341 attgaaaaag gtgctttgac aaaagattta aacgcgatac gaaatcattt ggataggttt
    89401 aagggtggca aaattttacg ttatatgaaa aaggctacaa ttgttaacct tgcaacaaat
    89461 gatttggggg gagcaagctg gagttcgcct ggtatccgag aaacatatga ggaactgatg
    89521 aaaggaaacc gattccttcc gactattgct gacagcacaa catttcagca ggccgtagag
    89581 gcatttcata aatacgatgc ttggaatcgt attccgaaac atatacaaaa attttttaca
    89641 acagcagatc ctaatgaaga aactgtaaaa atggatgaat atgaaaaaat gaattcgcgc
    89701 ttgtttaaat tgacatctag aataaacaca atatatcctg cagttataaa aaaggcagaa
    89761 caatttggct acaatgctta tattttatct gatacaataa cggcggaggc ggttgaagca
    89821 ggcaaagtca tcagctctat agcattaaac atccaaaaca tgaaccagcc tttgaagaag
    89881 ccgtgtgttt tgatttcctc aggtgaacta attgttactg tcggtaaaga gaatggtgtt
    89941 ggagggcgaa accaagaatt ctgtactgca gcggcattaa gaattgcagg aagttcaaaa
    90001 atagtaatag gatcagcaga cacagacgga actgacggac cgggaggttt tattgtggat
    90061 ggtgtccgga catgtcttgc aggggcaatc gtagatggtt atacttgcca ggaagctgcg
    90121 gagaagaata ttaatctcga agcttcattg aaaagacatg atacatcatc agatttatgg
    90181 gatcttggct gtggaattaa atcaacccaa agcataagcg cattggattt acgtattgtt
    90241 gctattatgg aatagaaaat ccaaattatg agagaagaga tgaatatgag tagaattgag
    90301 gatattagag catttgaaat gcttaatgcc agaggtaaac cgacaatttc ggtggaattg
    90361 acaacttcaa atggcatcgc tgttgaagcg acaaccccta ccggaacttc aaaaggaaaa
    90421 tatgaagcct atgaactata tgatggcgag aaacgatatc aaggacgagg ggtaaaaaaa
    90481 gcagtagaaa acgtaaacgg aataatacgc aagacgctta taggaatgga tgttagcaat
    90541 cagtccgcaa ttgatcaatg tatgattgaa ttggacggga cgaagaataa aagtagattg
    90601 gggggcaatg caattcttcc ggtttcggta gcggtctgta aagccggtgc agctgaagca
    90661 ggtctgccgt tatatcgtta tatcggggga ttatcagcaa aaagaattcc aaatatgata
    90721 tcaacggtta ttaccggtgg tgaatattcc ctttccggat tggaattcga ggactacata
    90781 ttagtttcag atcggaaaga aaagagtgtg gaagatggtg ttgaaatgat ttcctccctg
    90841 cgcaggcgtt tggaggaagt actaagggca aagtacgggg cgttccctga tacgacaggg
    90901 gctctggcac cgccgttgtc atcatcaaaa gaggcttttg aagtaatgtt gggtgtgatt
    90961 caagaacttg gatatgacaa ttattttact ctgggtttgg atgttgcagc aaatgagcta
    91021 tttgatgaaa gcaacggcag ttatcgatta atggatcgta tagtatcccc ggaggaatta
    91081 ttggaatatt attgctccct ggtatcagaa tatccaataa cctatatcga agacggtttt
    91141 gagcaaaatg actttaaaca ttttgcccaa ctaaaaaaag ctgttccaca gatacaaatt
    91201 gtcggtgacg atttattcgc aacaaatatt gagcggctca aagaaggaat aaacaatgac
    91261 agtgcgaata cgctactctt aaaaatgaat cagatcgcta cggtcagtga aacgattaag
    91321 acatgcaact atgcgcatca gaacgggtat gatgttacag catcattacg ttcaggagaa
    91381 actattgatt catttgttgc ggatcttgct gttggtctgg gtactcatca gattaaactt
    91441 ggttcgcctg taagaggtga gcgaaatgct aaattcaatc gtttaatgcg tattaattat
    91501 gaacttttat cagtgggatt ataagatgtc aataagaagc atttgtttgg aaggttgcaa
    91561 tagaatcttg cttaaatgga aataaaaatt gtaaggagta aatgataatg agtttagtcc
    91621 aaatattctc tattattatg gtaattgcag gcgcattggg catttatgga gctttgcagc
    91681 ttgatatagt ctacaacaac attgtaggtc cggctgttat gccgttaata tactctattg
    91741 ccttattagc attagtggcg gtgtactttg cgaagggcag aaatgcacca aaaatagaat
    91801 ggaatatgat gctaaaaccg cctaaatcag acgcatggat tttttatatt ttaaatatcg
    91861 tgatgtttat attactatat ttattgggaa ctatacccag tattctggta tttagcgttg
    91921 gaacgagctt atgtctaaaa cggatgaatc ttgttaagac aattgttttc agtgcattat
    91981 gggtggcagc attatatttt atatttgttg tatatctggg ggtacaattt tcgacaggca
    92041 tattaattaa atagtattct atgaggtgat taaatggcag aaacattatc ttatttaatg
    92101 ttaggatttc aggaagtact tgtaccaact acattgctaa tgacgtttat tggagtagta
    92161 atgggcacag ttgttggtgt attgccgggt ttggggcctt cagcagcatt atcggtactg
    92221 ttaccgatgg tttatggcaa agaagcaatt ccttcattgg ctatgcttgc aggtatttat
    92281 tatggcgcta tgtttggggg aatgataact tcaatttcaa taaatgttcc cggggaaagc
    92341 gcatcggtgg ttaccagttt tgatggttat cccttggcta agcaggggaa agccggtatg
    92401 gctatgggga tttctgcaat ctcttcattt tgcggcggaa catttggaat aattctttta
    92461 acgattatgg gagctgcatt ggcaaagttt gcgcttgcct ttggaccgcc ggagtatttt
    92521 gctatatatc tttttacatt tgtagcaatt atgacaatag gaagcggcgg aaaaataata
    92581 gaatcagcta tagctctgat atttggctta ttagtttcct gtataggttt ggatgttatg
    92641 acaggtaccg cacggcttac ttttggttcg gtgaacttgc ttgcgggagt tgactttcta
    92701 cctgcagttg ttggcttgtt cggactttca gagatagtat taagtctttc tgagggtgag
    92761 aagatagaaa tcgataaaaa gaaaggaaat tataaacaat attcttttaa gaatgtgttt
    92821 cctaaactgc atgattttat tcactccctt ccggcgatta tacgcggcgg agtggtagga
    92881 tttatagttg gcgtactgcc gggagcaggt gcaactattg ctactttttt gacttataat
    92941 attgaaaagc gtgtttctaa agatcctgat agttttggaa aaggaaatat ccgtggtgtt
    93001 gcagtagcgg aagctgcgaa caatggcgcg gcatccggcg catttgtccc tctgctgact
    93061 ttaggtattc cggggtcagc cacaactgcc attcttattg gggcgtttat aatggttgga
    93121 ataacaccgg gaccggctct atttagcgaa catccggatg tagcctgggg tttgatttcc
    93181 agcatgtatg tcggaaatgt tatgttgata ataataacga cggcatttat tccatttttt
    93241 atctgggtat taggtatttc gcagaagacc ttacctatta ttgttgccag cttgtgtgtt
    93301 gttggaacat attccttgaa caattcagtt tttgatgtat tattaatgtt ggttttnnnn
    93361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    93421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagt attgcaatta cctgcggcag
    93481 cagaggaatt gataattatc cattaataat caaggaaatt gtacaatttt gcaaagataa
    93541 aggtgcaaac ccttttataa ttccggcaat ggggagtcat ggcggagccg ttgcagaggg
    93601 acagcgtctt atatgtgaaa ctaacggggt tacagaagag tattgcggtt gtcctataaa
    93661 agcaactatg tcggtgacaa gaattggcgt tacagaagac ggttcttttg atgtatgtat
    93721 tgatagttat gcggctgagg ccgatggaat catcttagta aatcgcatta aaaatcatac
    93781 tgctttgaga ggtgattatg aaagcggatt gatgaaaatg atggcaatag gactgggaaa
    93841 acagtatgga gcatccttat gccatcgtgc cggttacgaa agaatggcag agttagttcc
    93901 aatgattgga aaaacagtat taaataatgc atcggtactt tttggagtcg gtatcgttga
    93961 caatgcctat gataaaaact ataagatcgc tgttttgact aaagatgaga ttccgattgt
    94021 agaaaaggaa ttgctgaaag aagcgaagag attaatgcct cgcctattac cgggaagtgc
    94081 agatgtgttg gttgtggaca gaatcggtaa aaacatcagt ggttctggtg cagatcccgg
    94141 cattacagga agattaacct ataagtattc cggagttgaa tcttttcagg caggaaaagt
    94201 tgctgtttta gatattactg atgagacaca tggaaacatt catggtgtag ggtatgtaga
    94261 tgttataaat cgaagggttt ttgaaaaagc tgatttagat aagacgtatc caaattcaat
    94321 aacatcaaca actcttatgt ctgacaaaat tccggttatg atgaagaatg accgggatac
    94381 tataaaatgt gctataaaaa cttgccaacc ggcggacccg gaaaacataa gacttatacg
    94441 cataacggat actctgcata tatctgaaat tttgatatct gaatgtatgg ttgattccgt
    94501 gcgccacatc ccaaatttgg agattactga tgaaattata gattgggaat tcaacgaaga
    94561 aggtaatctg tggtagaaga tgactgtata attaaatcgc aatattatat aaagatttta
    94621 gggggtttac ctatgtatat taataaggga ctatgtataa agtgctgtgc ttgtattcca
    94681 tattgtccaa tgcaggcgat cgaatataat tctgaaaaga agcaagttga aattaatgca
    94741 aatgaatgtg tggaatgtga aatatgtaaa aatgcaaatg tatgtcccaa agatgcatta
    94801 attatggaag aattaaaatg gcctcgtatt ttgaggagtc agttttcaaa tcctttggtt
    94861 actcaccctg atacaggggt gccgggacgt ggtactgaag aaatgaagac gaatgatgta
    94921 accggcagat tcaaaaaagg tatggtagga atggctatcg agatgggtcg cccaggaaca
    94981 ggaacccgtt tcagcgatgt agaaaaggta gcgatggctg ttgctgaggc aggtgtgcat
    95041 tttgaaccgt acaatcccgt aacaattcta atgaccgaca aagaaaccgg aaaattgaaa
    95101 gatgatatcc taaatgagaa agttctgtct gcaattattg aatttgatat gccgattaat
    95161 aaaaccaaac cggtgctgga gaaactaaaa tctgttattc cgttaataga tactgtgttt
    95221 agccttgatt tggcttatcg cttaacagag ggtgacagca atttagcagt aaaaatagcc
    95281 aatgaggcag gctttaccgt atcaatcaat ggcaagacta atgttggatt aggcagacca
    95341 ttagcgaagg aggaataaaa aatgacacat actttacata gaaatggaaa ttttgaaagc
    95401 ctgtcaaatg attatataat ttttgctatt actgcacaaa ctgtcaatgc caaaggcagt
    95461 gcccgaaaat ttaaagaatt tgaggacatt gtattgaagt ataaccccat aaactatgga
    95521 gatatgaaaa ccgggaatat gttcaatata gacataagca agatacagga aggttatcgt
    95581 gataattcta tagtgcatgc agtattctgc gatgaagata ctgtagcaaa agtactaaat
    95641 gagctaaaag aagcagatct tggaatctct atagtagtat caggaatttt ggaccgtgtt
    95701 tcagaatgtt gccatgaaaa agggatcaag ccccatacta ttgaacattc tcttggtatt
    95761 cacgggaggg tagattactt gcctaatgat aacgttttgg aaataagcac tatgtgtggt
    95821 catggaatgg tatctttttc acttattgag tacctgagcg agcaaatact aaaagacaga
    95881 attaccgtgg aagaggctgc aaaaaagctg gctaagcaat gccattgcgg ggtttttaac
    95941 cctgccaggg cagaatccat attgagagct atgactaaat agtatgggtc ttaataggca
    96001 ttttgaaatg taacaaggtt ttattttatg aagagaattc ataagggatt cgaatccttg
    96061 agggcaattt gcactaagga aacgatcata tgcgagtatt ttaagagtat tgtgggaacc
    96121 cggcacggta gtctctattg ttacagaaag ggaggttcct ttcttaagaa taagaaactc
    96181 ttgattcggg cagcaaaata tattacaata atttcattgt ttggattcat aaaggtagag
    96241 gtgcagaatg agcaagttta gcttcggcat aattgaatat ctggcttatg ctcacgcaat
    96301 tacaggtgat tttggagtac aagggagaag tgtacccaaa attgaacatt atgtaaacta
    96361 ataagccaaa actttactga taaaaattta tagctattgc ccctgagtaa ggtttttact
    96421 taggggtttt ttgcgtttaa ggagaagatt attatgaatt atgatattca attaaaaaat
    96481 aacattaaca agaactatat atttacattg cttcagaata ttgacctgac caggggaata
    96541 tggatgatat accttgcagg taaaggtatg agcctcacac aattaggcct tctggagatg
    96601 atattccaca taatctcttt tctgatggag gttcccaccg gagctgtagc tgacattttc
    96661 ggaaggaaga taagcaggat actgggaagg gtgttgtcat tgatcagtgt tgtgatactg
    96721 cttgctgccg acggcttcct ttggtttgca atatcctttg tgttcactgc cctatccttc
    96781 aatcttgagt caggagccgg ggaagctctt atatatgatt cactgaaaga aatcggtgaa
    96841 gaggacagat atatgaagat atccggaagg aaggaggttt tttaccaggc tgcgggggtg
    96901 atttcattcc tggctggtgg ttatatggct accaagagct ataatatagc ttttgcaatc
    96961 acaattatca taggagcggc tgctgtattg cagtccttca gtttcaagga gccgaccata
    97021 ggcagaaaca aagaagaaca aaaagggaga aatatatttt taaatcagct taaagaaagc
    97081 ttgagagtag taaaaagcaa tcccaggata gtatctctca tagtttttac agaaataata
    97141 cttacttttt gcacctgtat attcttctat ttgcagaact ttttgaaagg agaaggctat
    97201 aacgaggcaa taataggggt tgtatacgct gcttcgtttg ttgctgcggc tttgaccgca
    97261 tcctgggtcc acaggataga gagagtgata aaggaacagg gaatactgct tataatacct
    97321 tttgtcacag tggcgtgctt atggggcata gcacttagcc cataccactt tgttttcttt
    97381 atactgctta cagttacgga agagatagtg tatatagcca tgagtgacta tataaacaag
    97441 atgataccca gtgagaaccg tgctactata ctctcttttt caagcatggt attcagcttc
    97501 ttcatgatcg cgatttttcc tcttgtgggc cttcttggag acaggtattc attgagttat
    97561 gcaatcaagt gtcttggtgg gctgggtatt gtatttgtgg taataaacag tgtattgctg
    97621 ctgaatccgg gcaggaaaat ccggaaatag cattctgtct ctgcctatgg agaaaactgt
    97681 ttcctttgca ggagtattat gagatgataa tagtgaataa atttgcattg gcggtgataa
    97741 attgaagctg gacagactgg tttccattat tgtgctgctg ctcaggaaag aaagggtaca
    97801 ggccagggag cttgcagaga tattcgaggt atctgtgaga accatcctga gagacataga
    97861 ggcaataaat cttgcaggaa ttcctatagt cacttaccag ggagcaaacg gaggaatagg
    97921 tattgccgaa ggctaccgcc ttgacaaaag tgtgcttacg gagaatgaca tgtccaccat
    97981 catatctacg ttaagaggta ttgacggaac aatacctgac agccggcacg gtatactgat
    98041 ggaaaagctc cggaacgctt tgccgtcatc acagcttgag gctctggatg ccaaggtaca
    98101 gcagcttatt attgatttat ccccctgggg ccctaatgaa ctgttgaagt atagtgtcac
    98161 atgcataaga aaagcaatag agaatcgcaa tgaaatcgag tttgaatata tcgattcgac
    98221 aggcaaaagg acaaatcgca gagttgagcc ctacaccttg gtgctgaagg ggcagaaatg
    98281 gtatctgtat gcatggtgtc atacaaggca ggatttcagg cttttcaagc tttccaggat
    98341 gagagggctt gaggtgacga cagcagtcta ccagcccaaa aaggtatctt tggaaaggct
    98401 tgattgggag gatccctgga gtacttccga aagtatggtt ttcctccggc tggtgtttga
    98461 aaaagagatg gaaagcattg ttacagattg gttcggggac aatgtcgaaa agctggaaga
    98521 cgggagactg ctggcaaacg ccgttctgcc tgaaaataac tggttgtatg gatttatcct
    98581 gagcttcggc gcaggagcgg aggtaataga gcctgctcat attcggaaaa tactcgcaga
    98641 aatttcaaaa cagatatatg aaaaatattc catggaaaca tgacatacag gtgtcatgta
    98701 tgcagtgtta taatcaagaa aaagtcaagg aggaaataat aatggattat aacatcgagt
    98761 tgaaagaaca agggcttcag cccgtattat ccgtcagaaa aacaacatct gtaggaaatc
    98821 ttccccggga gatcgggaag gcatacggaa gcattatgca gtacctgcaa gagctggggg
    98881 aacagccggc ggaagcgcca tatacggcat attataacct ggatatggag catctggatg
    98941 tagagatggg ttttcctgtt tcaaaacagc ttgcaggaaa gggagaaatc aaagcaggtt
    99001 caattccggc aggaaaatac gcggaatgtg tgtacaaagg gccttatgcg gaaatggcgc
    99061 cggtatatga agcaatgaac aaatggatag ccgaaaaggg cttggaagct gtcggaacat
    99121 cctatgaagt ctattacaac tcacctgcag agatgcctga aagcgagctt ttgacaaaga
    99181 taatgctgcc tgtaagatag agaaatattt ggaggtgttg ttatgaagta cgattggaaa
    99241 aaacaggata aagagctgta tttgcctaag aataagccgg aaattgtaat ggtgcctcct
    99301 atgtggtttt tcatgcttga cggcaggggt aacccgaata atgaggattt tgccaaggca
    99361 gtaggtgtat tgtattctct tgcctatgca gtgaagatga tgctcaagaa gggtgtgacc
    99421 ccggaagggt actatgagta ctctgttttt cccctggagg gaatatggga ccttgcccct
    99481 gaggcaaggg aactggaggc attggacaag gacagtctaa tatataccat aatgataagg
    99541 cagcctgact ttgtcacgga agaaattgca cagcgggctt tagaagccgc agcaaaaaag
    99601 acaaagcatc cgctgctttc aaaagtcagg tttggatgca tcgaagaggg attgtgcgta
    99661 cagatgcttc acataggcca ctacgacagt gagccggctt cctttgccgt aatggaggaa
    99721 ttctgcaggg ccaataacct gaataggata tccaaggttc acagggaaat atacctttcc
    99781 gatgcgcgga agactcccgc agaaaagctt cagaccgttc tgcggttcaa agtggagaaa
    99841 atctgaggaa gctttaattc cttaaataaa ggaggctgtt atatgtcaga atatgatctc
    99901 atactggacg gaagccatac gcctgaatat gatgaaatag ttgaatatat caatatgcct
    99961 gcacgggatt tatggaggca gatcaatggt tttatccagc agagatataa atccttaccc
   100021 aggataatgt acagcgtatg ctcaggaaaa cccggatgga atgtaaaata tcagaaatcc
   100081 gggaagtcca tctgcacttt atatccggag caggacggtt ttgttgcctt ggttgtcgtc
   100141 acccttgacc ttatgcctgt aattgaagcc ttatcgggag agcttactga agaaacccgt
   100201 caaactataa gatcggcaaa gccgttcaat ggaaccaagt ggttgatgct tcaagtaaag
   100261 aatgatttgc agcttgaaga tgtcaagcag ctaatttctc tgaagcaagg ggcaattgca
   100321 aaatagaaga ataatgcgac acgggacatg gggacgtttc ccctgtctcg cgtcgcatta
   100381 ttcttaaaag tcggttttta ccggttttcc gttttcgttc aaggtaatcc gagtaatctt
   100441 ggaaagctga ggaacaccgt caatctttac acattcaata agctgatata cctttgatgt
   100501 atccccgttg atcttataga catcacccag tatcttgaag actgaatagg gatggtcggg
   100561 atccttgagg gcgatgaagg atgagccctg cttcagcatc gcttctgcaa taagctttgc
   100621 atagtcaata ttgctcaggt ctgcaatcag gtagtagtca gcttcatctc caaggtcttc
   100681 aaagcccttt atctgcttta tcttgaaatt gctttttgct gttgcgctta tagttgcaaa
   100741 aagaggcagg attccgaatc ccttgtgccc ggaaataata atggagtcct ttctcattac
   100801 agcctcttca atgtaatttc cttgagcttt ggtcatgaac ttcgagtcag ccagtttttt
   100861 tataaacgga ttcatataaa tcactccttg ttcatgcaag taccgtccca cttgaggata
   100921 aggcttcttg ccgcttttgc ttcgtctatc ctcctgacta cagtgccatg gggagcggtt
   100981 tttaatatat cgggagcttc ctttgcttct tcggcgattt ttatcattac atctatgaag
   101041 ctgtcaaggg tctccaggct ttcagtttcg gtaggctcta tcattatcgc attatttact
   101101 ataagcggga agtaaatggt cggggggtga tagccatagt ccagaagacg ctttgcaaca
   101161 tccagagtcg caatatgatt gtcgttttcc agaagcccgc tcaaaacgaa ctcatgcttg
   101221 caaacctgat ctatcggaag ctcatagtat ttcttcagtt tactcatcat atagtttgca
   101281 ttaagaaccg ctttccggct tacatccgtt aagccttccc cccccattga cagtatgtag
   101341 gcataagcct ttacggcgac tccgaagttg ccgtagaagc tctttacctt gcctatggag
   101401 tcaggcctgt catagtccag tatataggta tcgcccattt tctcaataac agggacaggg
   101461 agaaacttag caagctcgct tttaacccct accggtccgc ttcccgggcc gcccccgccg
   101521 tgaggtgtgg aaaatgtctt atgcaggttg agatggatca catcaaaacc catatccccg
   101581 ggccttgcaa ttcccataat ggcgttggca ttggctccgt cgtagtaaag gagtcctcct
   101641 gcctcatgaa ccaggtttgc gatttgcttg atatttttct cgaaaagccc gagtgtgctt
   101701 ggatttgtca gcattaggcc ggcaatttca tcactcagca ctgccttcag cctatcaaga
   101761 tcaacagctc cgtttttgtc agattcaatt tccactattt caaagcctgc gacagctgcg
   101821 ctggcagggt ttgttccgtg ggcagagtcc ggcactatta tcttttttct tttcttgtcg
   101881 ccccggctct catgatatgc cttgataatc ataaggccgg taagctcacc gtgagcaccg
   101941 gcggcgggct ggagggaaac cctgtccata ccgctgattt ctgaaagcat accgtcaagc
   102001 tcgtacatca gcttcagggc tccctggaca gtagcttcag gctggagggg atgaattttg
   102061 ctgaatcccg aaagattagc catgtcttca tttattttgg gattatactt catggtacag
   102121 gagcccagag ggtaaaagcc ggtatccact ccataattct tatttgagag atttgtaaag
   102181 tgtcttacca catctacttc actgacctca ggcagctcag gttcactatc cctcataagc
   102241 gaagggggaa tcacactgtc aaggctgatg tcagaaacgt cgcaaggcgg caggctgtat
   102301 gcttttcttc cttctttgga aagctcaaaa ataaggctgt tgtaattttt catcatacca
   102361 cctccattac ttgtgccagt ttgtcaatct gagcttttgt ccgtttttca gtcacacaga
   102421 aaagcaagcc gttcttcagc tcagggtatt ccttatccag ctcaaagcct ccaagtatat
   102481 tatgcttcag cagctcgtca ttaatcttac cggcgggtgt ttcacccgta acggcaaatt
   102541 ccttgaagaa gggcttgtta aacagaggct tgaactttcc gctgcttgtc agctgcttca
   102601 tggcatagtg ggcctttttt gcgctttgtt ccgctgcttc cttaaggccc tttttgccca
   102661 tcgtagtcat gtaaattgct gcggcaagag cattaaggcc ctggttggag catatgttgg
   102721 aggttgcctt gtatctgcgg atatgctgtt ccctggcctg cagggtaagt acaaaagccc
   102781 tcttgtcgtc tgcgtcttcc gattgcccta caattcttcc gggcattttt ctcatcagct
   102841 tggaggttgt ggcgagaaac cccaggtaag ggcctccgaa gctgaggctg ttcccaagag
   102901 actggccctc ccccgcaact atgtccaccc ctgactcacc ggggcttttc agtattccga
   102961 gagaaatggg gtccgcgtaa tcaataagca atgctttatt tgcatgcacc attttttcta
   103021 tttcggtcaa atcctctatt ataccgaaga aattcggatt ttggactatg acaccggcga
   103081 tgcctgcttc cacaaggggc ttcagctttt caacatctgt tatgccatca gccatatcgg
   103141 cttcaatgac ttccacatcc cggaacctca tatagctttt caatactttt cttacttcag
   103201 ggtgcacggt tcttgacact attatcgagc tgcgccttgc attttcgaca gccatcattg
   103261 ccgcttcaac acaggctgta gctccgtcat acatggaggc attggtaaca tccattcccg
   103321 tgaggctgca aatcatagtt tgatactcaa atattgcctg gagcgtaccc tggcttatct
   103381 caggctggta aggagtatat gcggtataga attccgacct cattgcaaga tgctttacga
   103441 cagagggaat aaaatggtcg tatgctcctg ctcccagaaa gcagatcaga tcatccgaac
   103501 tcttgttttt attgctcaga cccttcatat ggtctgaaag ctcaatttcc gaaagaggat
   103561 tgttcagatt aagctttctt ccaagccgaa gttcccgggg tatatctgta aataaatcat
   103621 ctgttgattt tgctccgatg ctctcaagca tcagtctttc atcatcctcg gtatgaggta
   103681 aatacctgtg catattatgc ctccttatcg cagaattcct ggtactcctg ctcgctcatg
   103741 agttcatcaa ggccggcttt gtcagttatc tcaaccacta caatccagct tccataggga
   103801 tcctcgttta caagctgcgg tgaatccgac aatgcttcat ttacttcaag aacttttccg
   103861 gcaaccggaa gatatgagtc ggaagctgcc tttacagatt caataacgct gaaaacatcc
   103921 ccggcattga gttcgtcccc tactgccggc aattccacat agactatttc ccccaaagca
   103981 tgctgggcat aatcggtgat gcccacatag gccttttcgc cttcaaccct gacccattcg
   104041 tgatcctttg aataaagcaa tcctgatttt gtttccattt ttcattcctc ctgataaata
   104101 tatttttata ttattttctg tactttttag tatagaatct tctgtcaaca accctggcct
   104161 ttacggcatt ttttcttatt tggatttcta atggagtacc aagctcggag tattcgatat
   104221 caaccattgc aaggcctata ttcttcttta gggtaggcga actgtagccg gtcgttacaa
   104281 agccgataac cttgccgtca gataggacat catacccatg ccttggaatt cccggcccct
   104341 ccatctcaaa tccgactatc ttccttttca ggccttcttc cttctgcttt accagagctt
   104401 ccttacctat aaattcgggt ttccccagct ttacaaacat gccgaagcct gcttccaaag
   104461 gagtaatatc cggcgacagc tcatttccgt aaaggggaag gttggcctca aatctgaggg
   104521 tatccctgca gcccagcccg gctggtttta cacccttatc cttgcccgtt tcaagtattt
   104581 tatcccagac atgctctgag tcttcatgct tcatatagat ttcaaagcca tcctcgccgg
   104641 tgtagccggt gcgggataca aggcagtttt tgcctgcaat tttgacattt gtcttgaagt
   104701 agaagaaggc tatttcagag aggtttgtat cggtaagctc ctgcaatact tcctcggcct
   104761 ttggcccctg cagggctatc tccgatacat cctcggaaat atttttaagt tcagcgtcat
   104821 aattgccgat attatccttc atccattgga agtccttatc tacgttgctg gcattgatta
   104881 caagcaaata aaagtcattt gcgtgcttgt agaccagaag gtcgtctact atgcctccgt
   104941 tgggatagca catcatggca taaatgacct gattttcgtt cattaccgac acatcattgg
   105001 tgaccagatt ctgtacaaag tctgttgcct ggggccctct gacctcaacc tcacccatgt
   105061 gggacacatc aaatattccg gcgcaattcc tcactgcttc atgctctgct gttatgcctt
   105121 cgtactgtac cggcaatgcc catccggcaa aatcaattac tcttccgccg tatttttcgt
   105181 ggacatcgta aagggacgtt ttcttaggtt cgctcataaa ataatcctcc ttttagtaac
   105241 ttagtaatat gttatccaaa ttaaaaaggg atataatagc acaataaatt tatgtgccat
   105301 tatatccctc tgttcttttg cctgagagct tctacatatt tatgtatttc tcctacggcg
   105361 ccgataaacg gtctcttcag agtaccatcc cgtggcgatc cttttgcctg aaagtgtctg
   105421 tgaagagggg ctgctcttca cattgcttct tcggctacct tacaaggtat ctctcgccat
   105481 tttcatctgg ttcagcatta tttaattact aatataatac aatgcatagc aaatatttac
   105541 aacccattga tatttattta actttatggg agaagggtta tcccagaaat tccggatttg
   105601 ttacatggtc cgatcttacg gctttttgct ctatggtgaa atttattgta ttgctgcgct
   105661 catccagagt acaggggaat tttgcaccgc attgctcgca ctgcagatat tcatggcttt
   105721 ttatcagttc tctgttatcg aataaaaaag gcagtgcttc ggattttttg ttctcaaggt
   105781 catagctgta taaataggtg gcttcacgct ttgcggtaaa atgcctgccg ctgcattttg
   105841 tgcatacaag aggctctttt aattccaata tgcatcttcc tttcttaaag attatggcaa
   105901 cagtattatg gtatttaata tttgataaaa tatgcttata acaagatttt aatgagggtt
   105961 tttgcataca tgcaaaaacc tacccgagcg tttcaacaag gcgagagata tgctcactgc
   106021 ctgttagaca atatcttttc gccttgttat aacttataat aagaatattg aacaataagg
   106081 aggaatacaa aatgaaaaaa ataactgtat tcggcagcag gcattgaccg gattgccagc
   106141 cattgaaaga gtttctttca aataatggga ttatctacga atatattgat ataacagaca
   106201 gtatgaagaa cctgaaaatc tatctcaagc tgcgggatac aaggcctgag tttgaagaaa
   106261 tcaagagaat cggcagagtg ggaataccct ttataatgct tgataatggg gagaagctca
   106321 tatttgatca gccggaattg gatgaattga aataacgaat tatttttata tacatgcaga
   106381 aaatatctgt atgaaaaaaa gatttgttat atatggactg gcaggatgga tagcggagac
   106441 aatatttacg ggattaggat ccctgttcaa gggcgatttg acaatgcata catggactta
   106501 tatatggatg tttccgatat atgggcttat gatactttta gagcctgtcc atgataatat
   106561 aagaaacgct ccggttgtgg caagaggggg tgtgtacact ataatcatat ttggaataga
   106621 atacacaacc ggatggattc tcagagagat tataggaaag tgtccatggg attacagtgc
   106681 atggccatat tctatcgacg ggataatcac cttatccttt atcccggttt ggttttttgg
   106741 agggctcata tttgaaaagc ttcacgacta cctgatacgc cgcaaaattc tgatgtagcg
   106801 gattaccagg cggcgattgc gccgtctgtc cttggctcag tgccgccaac caaaatattt
   106861 ctctcgtccc gccatatgat ctgtcctctt ccaaagctcc cggaataagg attaattttt
   106921 atattatgtc ctttattaat aaggccctgt acagcatcta aggggaaacc ttcttccact
   106981 tcaatatcct tttccgcaat ccactgccat ctgggtgaat ccaggcaatc ctggggattc
   107041 atatggaagt ctaaggtatt cataatcacc tggacatggc cttgaggctg cataaatgca
   107101 cccattactc cgaaagggcc gatagctctg ccttcccggg taaggaatcc gggaataatt
   107161 gtatgataag gcttcttccc cggggcaatg cagttttcac gggacggatc agttgagaaa
   107221 ttgcagcctc tgttatgaag gctaatacct gtcccgggta tgacaatacc cgagccaaag
   107281 cccatatagt tgctttggat atacgatacc atattgcctt ctccatcggc tgccgccagg
   107341 tataccgtgc ccccttcggg aggcctgccc ggcgtgggta tcagcgcttt gctgccgata
   107401 cacttccttc tttcggcagc ataattatca gagagcagtt cctcagtgga taccttcata
   107461 cttctgcgat cagctatata ccttattccg tcggcaaatg caagcttcat cgcttccaac
   107521 tgatgatgat aagtatcaat ggaatccctt agttcaaaat caaatccctt taatatattc
   107581 aaagtcatta aagccactat tccgtgcccg tttgggggga tttcccatat gtcataccct
   107641 cgatagttaa tacttatggg ttcaacccat tccggttcaa aagactcaag atccttcagg
   107701 gacaaatatc ctccggtttt tccggcaaat tcggaaattt tttctgccaa ggctccccga
   107761 tagaagcttt cggcatttgt ttcacctatc tcctgaaggg tttttccatg ggcggatgaa
   107821 ctccacagct ccccggcctt tggcgctctg cctgatgggg caaaggtatc gaaccagtgc
   107881 cggaataatt cgtcctttag ggctattcta taggtcctgt atgcgctttc ccaggatttt
   107941 gcgacggtag gtgcgacagg atgcccttca taagcataag caactgcagg agatagggct
   108001 tctgaaagag gaagcctgcc aaaacgtttt gacagcgccg accatgcgga gggtataccg
   108061 ggaacggtga caggcatcca gccatgcttg ggaatgccgc ggctttggat tttttcgatt
   108121 atttcggcag gaatggaagc cggtgcaggc ccgctggcat tcagcccatg gagttttcct
   108181 tctgaccata caagggcaaa ggcatctcct ccgataccgt tggaggtggg ctccactaca
   108241 gtaaggcagg cagccgtcgc aactgcggca tcaatggcat tgcctccttt tttgagcatt
   108301 tccaggccgg cttgtgctgc caggggatgt gaggcggcga tcatgccttt tcttgcatat
   108361 ataatatttc ttcttgaagg gtaattatat ttatgggcat caaactgcat ttccattcct
   108421 ccttttgaat gctaaataaa ttataaaaca gcctatgttg aaaagcaaat gcatggagga
   108481 cacggggacg tgggacacgg ggacgtttct cctgtctcct gaaagcggga caggagaaac
   108541 gtccccgtgt cctccgtccc cgtgtcccat gttcttgatt ctataatagt aatacccggg
   108601 aaattatatt ataatattaa aggacgagac caatcagcat ggaaataaac agcccctgtg
   108661 aaggagggaa taataattgg cagtcttagt aacgggaggc gcaggatata ttggttccca
   108721 ctgcgtctgc agtcttattg acagaaaaga aaaagttgtt gttgcagata acctgcaaac
   108781 aggccacgag ggtgctgtgc atggagaagc aagattttac aaaggggata tccgggatgg
   108841 gaaatttctc gataagatat ttaatgagaa tgatattgat acggcaatcc attttgctgc
   108901 cgattccctg gttggcgtga gcatgaaaga gcctctttct tactttgaca ataatgtata
   108961 tggaacagaa gtactgcttt cagcaatgaa caggcataat gtgaacagaa tagtattttc
   109021 atcctctgca gctgcctacg gcgagccgga gagcattccc attctggaga cggacaggac
   109081 tgaaccaact aatgcctatg gggaaaccaa gcttgtaatg gaaaaaatga tgaagtgggc
   109141 ggatattgcc catggtataa agtatatagc cctcaggtac tttaacgtag ccggagccca
   109201 tgaaagcgga agaatcggcg aggaccactg ccctgagaca catctgattc cgcttatact
   109261 tcaggttccc cttgggaagc gggaaagcat ctcaatattc ggtgatgatt atgatactta
   109321 tgacggtacc tgtataaggg attatataca tgtaacggat ttgacaaatg cgcatatcat
   109381 ggctcttgac cgcttgagag gcggagctgc aagcactgtt tataacctcg gaaacggtga
   109441 aggcttctca gttaaggaaa ttgtcgaggc agcaagaagg gtgaccaatc acccgatacc
   109501 ggcagtaata tgccccagaa gggcaggaga cccttccaca ctgatagcct ccgcaaaaaa
   109561 ggccggggat gagatgggat ggcagcccag gtacaatagc atcgatgaaa ttatagcgac
   109621 tgcatggaag tggcataaga ataatccgga gggatttaag ggaaagcgaa tcgtagaagg
   109681 agatagacag aaatgaaaga aatcataaaa cgtatggcaa aattgttctt tgggttattt
   109741 ttgtatggtt tggggattgt tatgacaatc aatgccaatc tgggtttggc accttgggag
   109801 atatttcatc agggattgtc aataaaaacc ggaattacaa tgggtcaggc aagcatatat
   109861 gtcgggatgg tccttgtagg acttgacagt ctgcttggag aaaggctcgg atggggaaca
   109921 ctgttcaaca tgctattcat tggattgttc atggatatac taatgctgaa tcacctgatt
   109981 ccggtatttc agtcagtaat actaagattt gcaatgatgc ttctgggcat gttcgttatc
   110041 ggaatagcca gctatttcta cataagcgtc ggattgggct ccggcccaag ggacggtctg
   110101 atggtggcgc tgacaaaaaa gaccggcaag tccgtcaggt tcataagaaa cagcatcgaa
   110161 ataaccgcac tggccgcagg atatatcctt ggaggttctg tgggaatagg aactgcaatt
   110221 atggcaatta cagccggata ttttgttcag ttttccttta agcttttcaa atataacctg
   110281 aaggagacta agcacagatt cattgatgag gatttaaaat tcttgtacga cagggttcgc
   110341 ggcaaaaagg aagacagaaa agcagaagac caatagttat actgtcaaaa aggagaattg
   110401 caatgtcttt gaagagtttt ctcaaactgg ttgaaataca gacaaaagtt gcaagcgtaa
   110461 taccctttgc attgggaacc ttatatacag tttatcactt tggaatcttt aatcccagga
   110521 acttcataat aatgttcata tcattgctat cctttgatat ggcaactaca gcaattaaca
   110581 attactatga ttacaagaga gcgaacaaga cccagggctt taattacgaa agtcataatg
   110641 ccatagtaaa atgcaatttg aaagaatcaa ctgtaaaact aacaataacc gctttgctga
   110701 ctgttgcatc tgttttcggc cttatgcttt ttcttaatac atctcccgtt attctgctgc
   110761 tgggggtttt gtcctttgca gtaggaattc tgtattcctt cggaccgata ccgatttccc
   110821 ggacaccctt tggagaggtg ttttccgggt tgttcatggg gtttgtaatt actttcatat
   110881 caatatatgt acatgtatat gacacgggta ttgctggtct gacatattcc ggcagtatgg
   110941 tgggaataag cttcaacctg actgaaggaa tatatatatt tcttctgtca ttacctgcag
   111001 taaacggcat tgccaatatc atgctggcaa acaacatatg cgatattgaa gacgatatcg
   111061 agaacagacg ttatactctg cccatatata taggaaagga aagggcttta tgggtattta
   111121 aagccttata ctacatcagc tatgcagatt tgctgctgct gcttgttctg ggagttaccc
   111181 ccgcggcaac agtcattacg ctttttacaa tttttcccgt aagcagcaat ataaaaagtt
   111241 tttttgaaaa gcagaccaag aaggacacct ttatattgtc cgttaagaac tttctgatta
   111301 taaatgtggt acaagtgctg gcaataggaa ctaccatcat tattgaagga cttatatagc
   111361 aaaaagggct attgcacaat agtagaataa tgcgacatat ctgaggtttg cagtcatcct
   111421 gcagagaacg cgcatatcaa ggcacataaa acagcatccg aatccctaag tggcctttgt
   111481 gtatcagagg acgagattgc aaggctgaat actattatat cgccccttgc tcagaaagcc
   111541 aatctgttca tcaaatatcg ctgctcacaa atctgaacaa ccttacaccc tgcgaaacgt
   111601 tcagctttta ccacggagaa gaggttttac ataaacttgg atgctcatcg gcagccgccg
   111661 gtgatatcat gttaaaaccc gctcttctca aaaaatagta tgaactgtgc ctcgctatat
   111721 tcaaaatttt tgctataaat attgcttata attcttgata acttgcaaaa cctgcgatat
   111781 tattgaggca atgggtatgc agaacgaaag tctcagcaac ttcggtaacg aattggaggt
   111841 aaagcatgaa ggatcaggca gttgtttcgg gcagcagaag gggatattta aataaggatt
   111901 ttgagttttt tcatttaaaa gacaggaaaa ttatggagtt tgaattgcac tttcatgatt
   111961 ttaacaaaat aatcatcttc atgtcgggaa atgttacgta tctgatagaa ggcagggcgt
   112021 acaagcttaa accctgggat atgcttctta tcggcagcag cgagatacat aaagcaatag
   112081 taggcactag cgggacttat gagaggatga taatatgggt caattcaaat tttctgcata
   112141 agtataaaag tcctgagggt gatttgttga gatgctttga tattgcctca aaagagaagc
   112201 gcaatatact gaggctgaat cctaagcttc tcaggcaggg gaaacatatt ctttctcagc
   112261 tgaaggagga atgcaagagc ggcggttttg gaagtcaggt gcttaagaac tcattattcc
   112321 ttcaattcat agtatatctt aatagaattt atcttgagcc tgattccata gagcataagg
   112381 ctgacgggga ttatgacgaa agcataggag aagtaataag gcatataaac gagaatattt
   112441 gtgaggatat gccaatagaa ggcctggcat ccaaattcta tatgagcaag tatcatttga
   112501 tgcataagtt taaaagccag acaggttata ccattcacag ctacatcctt caaaagcggc
   112561 tgatgatggc caatacactc ataaagtcag gaaaatcagt agcagaagca tgtacggaat
   112621 gcggctttgg cgattattca agcttcgtaa gggcctttaa aagaatgtac gggctttcac
   112681 cgaagaaaca tataaaaatg gtggaggaac tggaaaaggc ggggaaataa ttactgtaaa
   112741 acattttatc tgacaaaata gaaggaacat tgacatttgc atttttaggg cttaatatga
   112801 ttatgatttg cattttaaat aaatgctaat aagattaatg gaaatggggt gtgatgctta
   112861 tgaaagccga aaatggcaag ctgaagctct atggcttcaa taatcttacg aaatctttga
   112921 gttttaacat ctatgatatt tgctatacca aatctagaga agaccgtaag aaatatattg
   112981 aatatattga tgaacagtat aattctacaa ggcttacaaa gatactcagt gatgttaccg
   113041 aaatgatagg tgccagtgta ctgaatgtag ccaaacagga ttatgaccct cagggagcca
   113101 gtgttacact tcttatttcg gaagaagagg ttcctcttta tgtgctcgat ccttcctgta
   113161 acagaggaat aataacccct atcagggaga atattgtggg acaccttgac aagagtcacg
   113221 tgacagtaca cacgtacccc gagagccatc ctcagaatga gataagtacc ttcagggttg
   113281 atatagatgt gactacctgt ggaactattt ccccactaaa ggcattgaat tatcttatcg
   113341 ggagctttga ttcggatata attactatcg actacagggt caggggatat acaagaaata
   113401 ttcgaggccg gaaacacttc atagaccata agataaactc aatacaaaac ttcattgcca
   113461 aggacacaat aaacagatat gaccttattg atatgaacat ataccagtcc aatatattcc
   113521 atacaaaaat gaggataagg gatttggatt tgaacaacta tttatttgag atagaggaag
   113581 atgacttgac tccaaaggag aaaaaggata tatcgaagaa gctggacagg gaaatcacag
   113641 agatttttta cggaataaat ctgccaaaga tttaatatta agaatatatt taggaatagc
   113701 aaacaagcta ttctttttgt ttgacttata cgtgaataga tggaataata ttatttacac
   113761 attgaaataa ggagcatatg atgttaagct ttaagaaatt tatatataat acaataaaaa
   113821 agctttatat ttcttcggag ctgttgaaat ccgaagaaaa aaaactgctg catatttctg
   113881 atacccctct ttgtttttat tctgaactca aaaggcttat tggatggctt aagcctgcat
   113941 atatagtgca tacgggggac cttacggaca atataaagct ggagctttac cctgcattga
   114001 tccatgagca tgagaagggg gtaaaggttc tggcagacat gcttgaggct tccggagcag
   114061 aagtaatatt ggcaattggt aaccatgaca atattgatgt tgtcaataag cattttgtaa
   114121 gaagccgcat aataatagaa gctgagacaa ttaatttaga gggattgagt tttagaatca
   114181 gccattttcc taaaggcgtt caagaaaacc cgtcccggta taatctgttt ggacatgatt
   114241 taacgcttaa gaatggatgt attgatgata aactgtattt taacgggata acatatataa
   114301 gcattatagg gttagagtca ggagagttca cgactctaaa ttatccttta tatacaaatg
   114361 acgcaagggt aggaagaagg aaagtgggac tgtaaaaaaa taatatcaag ggagggttat
   114421 ttatgctcag cttggttaga gggggaccca aagtattact tcttgaaagc agcagaatag
   114481 aagaattaag gaactacttt attcaagaga agggcgcaga agcttataat ccaagtgatg
   114541 tatttgaaaa tgcaggggaa gaccatacaa ttctttttat cattgataaa attaaagaag
   114601 cagttgattt tgatgaagca gataacggga ttttaattaa tgaagaacct gatgtaatac
   114661 tatgcaatat gttgaacgat aaaaagattg atcttgtaag caggtcacgg attgcaacga
   114721 ggataatagt attaagagca atgggggacc tggaaagggt agtaagggat ataagcgggg
   114781 actatcaatg tattgtggga agcccgctgg atattattaa atccagtgat gagaagggga
   114841 ctctggtggt tgttacagaa aaacccctgc atagaaacat aagttataag aaggatattt
   114901 atgaaaaggg tctgcttatt aatgaaaaat acaagcctct tatacgaagc ctcagatccc
   114961 gggcattaaa atacctgaac aacggtatag gcaataagga ctggtatgag cttgaaatca
   115021 ggatatatga caaatatggg gagtatgacc tccattacac aagactcatt aatataatgg
   115081 aatccctgga gattggaatt atactggggg catcctgggg aaaagactat ccgaggccta
   115141 tgatggcgat tgagatatac agagtaaggt tttttaccta ttatgacccg atatatataa
   115201 aaaagttatt gatgggtttg gaattcctgg aggatggaac cagaatagta gactatgacc
   115261 ttttttataa gagaaaaaag ctgtattggg ctgaaacggt tgaaaaggat ataaaggaga
   115321 ggcatctgat agccgctaaa tacaggaaag aaatattatc aagattaaac gaagaagaaa
   115381 ttaataagat gtatgaaatt gaaaataaca tattggctac cagaaaataa ccggataagt
   115441 atggacttgc agcttggacc tgctccatgc atataatgta agaaagtgtg catgggagtg
   115501 aatatatatg tatgatgatg aaaaagacga tattgaactg gactatgagc tctgtgaaag
   115561 ctgtccgttt cacaggcagc ggccgccaac cttcgggcct tttggaccgc aatttcaacc
   115621 gccgggtgta gggccgggct cttcggatgg ccccccatca gggccgccgc cttctataac
   115681 accacagttt ggggggccgc agggtatagg gactctggca gtagatcccg ggtcaataag
   115741 aagatgcaga ttcagtttcg tttacttaca gctcagaaat ggcagggaat tctggatgtg
   115801 gctgacattt gtgggtagac gctcaatttc aggctggagg tggacgggac gcaggtgggt
   115861 attcttcgga gttgacctga ggcagataga aagcttctat tgctattaaa aaggtataaa
   115921 agatatgttc agcagctttg ccgggcatat cttttttaat atggacaata gaagaataat
   115981 gcgacgggga cgcggggacg tttccccgtc gcattattct tattttgcga agaatttagc
   116041 tcccttattg atgcctctgc atattgtgta gtaattacat tcttttataa ggaggaggct
   116101 caatggggat tacacttaca acactgcatt gggtgtactt gatcttcatt ctgctggtaa
   116161 ttgccttcat tgtaatgagg aaggatacca gcttcatctg tgttgccgga attctcgttc
   116221 tggggtttat agcaacctcc acaatcactc attcggtaac cggggtcttt aacagttttg
   116281 catacgctat taaggaacta ttggggacaa tattgattat ttcgattatt tacggaatga
   116341 gtaaagtatt gatgaaaaca ggtattaatg aagctatggt atacccattt acaaggctga
   116401 tacgtactcc ggccctggca tactgggtta taggcatact gatgatgatc atttcctggt
   116461 tcttctggcc ctctcctgct gttgcgctga tgggggcagt acttctgccg gtcgcactta
   116521 aagcaaagct tcccgccatg ggcgtagctg tggctatgaa ccttttcggc cacggcatag
   116581 cattatcagg agactttatt atccagggag cgccgaaatt aactgccgat ggtgccggga
   116641 tacctgtaat cgatgtggta gcggccagtg tgccattggt gataattatg ggtgcagtca
   116701 caaccatctc tgcattctgg atacttcgaa aggacttgaa gtccggagca ttgaaggttg
   116761 aagatgaaaa catgagaaaa agcatgattg tgcaaatcaa cgacggtaag gcaagtatga
   116821 aagaaatacc tcctgttgta aaaaaactcc ttgctgcgat ggtacccgta ctgtttgccg
   116881 tagatatatt gatgatgttt attctaaagc ttcagggcgg ggatgcaaca gctttaatcg
   116941 gcggaacatc agtactgata ttgataattg tatccttctt tgcttatagt gcagatcctc
   117001 tgggcaaaat tacagagcat ttaatagaag gattgcagtt cggcttcagg gtgttcgggc
   117061 cggttattcc tattgcagca tttttctact tgggcgattc ggctataatc gatatattcg
   117121 gcaaggtgct tccggaaggc tcaaacgggc ttgtaaatga cctgggtgtt gccttggcag
   117181 gcgcagtgcc gctgaaccgg ggaatcggag ctgtcacagc cacagccgtc ggtgcaatta
   117241 caggacttga cggttcagga ttttcaggca tatccctagt cgggtctatt gcaaagctat
   117301 tttctatagc cttaggcaca ggagcggcga cgcttacagc cttaggccag gttgcagcca
   117361 tctgggtagg gggaggcact ctgatacctt gggcggtaat ccctgcggca gcaatatgcg
   117421 gagtcgatcc cttcgaattg gcccgccgca atctgaagcc tgtagtaata gggcttataa
   117481 taactacaat agccgccgtg tttttagtat aaattcatat aaatcagccg catttgcggc
   117541 tttttttctt gtcatttgga agaataagaa ataatatata tacatatcag aaaaaccgga
   117601 ggttgtgtga atgaaaaagc tcaaggcagt aataatcatg ctgatctgca cagcaatagc
   117661 tgctgcagga tgtagtacgg cagacaataa ggaacctgat ataactgaaa tacccaagct
   117721 gaaaatcggg gtaatgcccg cagcagactc cgcaccgata ttccttgctg aagaaaaggg
   117781 atactttaag gaactgggat tggatgcgga aatacaggta tatacaaatg cggctaacag
   117841 gcaaagtgcc ctgcagagcg gagagcttga cggtgctatg gtggacttga ttgcatttgt
   117901 gaataatgta cataacggct ttgatataaa aataaccaca agtactgacg gaagcttccc
   117961 atttctcgta aacaaggatt taaaggaaaa gaagaatatt aaagtaggaa tgatggaagt
   118021 gagcgttaca aattttctgt cggaggaata cttgggggac aagtatgaaa tggagaaggt
   118081 atatatcaat gagattcccg caaggctgga gatgataaaa gcggggaatt tggacattgc
   118141 caatattccg gaacctatgg cctccatggg agaacttaac ggactaaaaa aggttgtcta
   118201 cgagaacaag gatgacttta tgcctgaggc aatggccttt acgggaaaag cactcaaaga
   118261 gaaggaaaag gcaattgagc tgttccacag agcctttgac aaagccgtag acgatataaa
   118321 aagggatgac acccttgcaa gggaagtact gatagaaaaa ctgcagctta aaccgggaat
   118381 aaaggataaa ataatactcc ccgtgtatca caaaaccagg cttcctgatg aagcttacat
   118441 ccgcaagatt gtggagtggg ttgagaaaat ccagaatatt aaggtgaatg taaagtatga
   118501 tgatatgatt gaagggaagt tcattaacca atgatagaag ttaacaacct gcaggtgtct
   118561 tatggaaatg tgcgggcgtt ggaaggaatc agcctgaagg tggagcgcaa tgccacctgt
   118621 gcgataatcg ggccctctgg atgcggaaag actaccttgc tttatacatt agcggggctt
   118681 atccgtccgg aggagggaac aatatatatc ggcggtgaag agctttccgg cataagaaga
   118741 agcaccggaa taatactgca ggactatggc ctttttccat ggaaaaacgt ctggaataat
   118801 gttgctctgg gcctgcaggt aagaggtgaa accaaagaaa aaatcagtga aaccgtaagc
   118861 aatatacttg cagtgcttgg aatcggacat cttagctgta aatatcccgc agagctgagc
   118921 ggaggagaaa aacagagggt ggcaataggc agaacccttg caatgaagcc tgacctgctt
   118981 ctgatggatg aaccttcctc agcccttgat gccattacaa aggagcatat acagaacctt
   119041 attctgaagc tgtataaaag tgctcccctg accatggtca ttgtgactca cagcattgag
   119101 gaggctgttt tccttgggca gaaaatcata gtaatgggca aggcaaaaat aaagcacgtg
   119161 ctggacaacc cttatttcgg tgacgaagag cttcgaagca ggatggagta ctgcaaaata
   119221 tgcctagaag tgcgcaaatg gatggatagg ggggaataag ttgataagaa gcaaaagcct
   119281 ttatggaaca cttatgattt ttcttttctg gtatatattg tatattatgc ttcaatcccc
   119341 tgttatacca agcccctttg ctgcagtatt gaactttata acgatacttc ctgcggtgct
   119401 tctgccccat gaactggcca gtttggccag gataattgcc gcagcagcct tttccctgct
   119461 cctggggttg ccgctagggc tgcttacagg tatgaacaat aaagccgaca ggcttataac
   119521 acccatagta tatatacttt atccaatacc aaaggttgct ttcctgccgg tattgatgat
   119581 tttgttcgga ttggggaatg cgtccaagat aattcttatt tcaataatag taatatttca
   119641 aataattgta accaccaggg acggagtgca tgaactggga aaggaatact ttctttccgc
   119701 tgaatcctta gatatgagca gggcacagct gtacagacac atcataattc cggcaataat
   119761 gcccaaaata ataacagcct tgcggcttac agtgggaacc tccattgcag tgctgttttt
   119821 cagtgagaac tttgccgcca gatacggtat aggatatttt ataatgaaca gttgggttgt
   119881 cgtcaactat gtggagatgt tcagcggcat tttggctcta agtattatgg ggctcttgct
   119941 gtttaagata attgacaaat tggaaagcag attctgcgca tggatggagg tatgaaattt
   120001 tacaaattgt ttgactataa ttagatttta ttatattatt tgaatagata tatttttgat
   120061 atattataat atttttagag ggaaatccgc cactttcaga agtggtggtt atactgtaat
   120121 tggatttttt gttgttgatg cactatcaaa tattggaggt ggttatagaa ggatagtttg
   120181 gcattatcaa gatagctcaa atggggaagg gctatataat ggtcagttcg aaaatgagaa
   120241 ggaggaatag tatgaaaaag ttattatcct tgactttagt tgttgcgatg gcactatcct
   120301 tgcttacagg gtgtgcacag cctgctccgg ctccaagcgg tgagcctgca gctccggctg
   120361 aagaagaagt tattaagatc ggtgtatttg aacctttgac aggtgcaaat gctgccggcg
   120421 gtgagcttga aatggaaggt atcgagcttg cccacgaatt atatcccgaa gtattgggca
   120481 aaaaggttga gcttatagta gttgataata aatcagacaa ggttgaagca gctacagctg
   120541 cagccagatt ggttgagaaa gaaaaagtta ctgcaattat aggaagctgg ggaagctccc
   120601 ttgcaatggc agcaggagat atcgtaaaaa atgcaaaggt tcctgcaatg ggaacatctt
   120661 gcaccaatcc tcttgttaca cagggtaatg attattactt cagggtttgc tttatagatc
   120721 ccttccaggg agtagtaatg tcaaactacg cttacaatgg ccttggtgcc aagaaggcag
   120781 ccattgtgca ggaagtatcc aacgactatg cagtaggact tgcaaagttc tttacagata
   120841 acttcaagaa gctgacaggt gatgaaaaca gtatagttgc agttgccaac tataatacag
   120901 gagaccagga cttcactgca cagcttacaa acataaaagc acagaatcct gatgttatat
   120961 tcgctccggg taactttaca gaatcagctc ttcttataaa gcaggccaga cagcttggaa
   121021 taaagactcc gtttataggt ggagatactt atgagactcc ggaatttgct gaaataggcg
   121081 gagatgctgt agaaggtgtt gtattctcaa cattctttgc aacagaaaga ccgataactt
   121141 cagaatcaac aaaattcctg gatgcataca gggcaaaata tcccgggaaa gaacctgcag
   121201 cagttacagc ccttggatac gatgcatacc ttattctcat aaaggctatc gaacaggcag
   121261 gggcagccga ctccgcatca attcataatg cactgcttaa tataaaggat ttccagggtg
   121321 cagctggtgt tgtaaacttt gatgaaaacg gagatgctgt taagagcgct gttataaaaa
   121381 cagtaaaaga cggcaaattt acattcatgg atgtagttga accctaaata aaaatcaacg
   121441 gatggataaa ccttccccat atcagtaaat ataaaaagta gagtaaaatg catttcctcc
   121501 cttaaaggga ggaagtgcgt tttatgataa gacttaacta aagtaccttt caacacctaa
   121561 agctgcatga tacctgccta tttgctccac tactgcgttg atgtcaatcg ccgtagccac
   121621 tgctgcgcct ctttccatcg ccttgtattg gaatcaaata ttctgcgtat cataatacaa
   121681 caattagatg ttacaaggta ccaggaattt ttttatgtta taaaattaat gctacattgt
   121741 tcggaagagt atgttttccg gcataatgag aaatgaatgg gaaggttccc tgacgaaatc
   121801 ttacttaggt ggtgtatata tgacactgga attgttctta cagcatttgg ctaatggtat
   121861 atcactggga agcctttatg cactggttgc cataggatac acgatggtgt acggtatatt
   121921 gaggctgata aattttgccc acggtgatat attcatgatg gcagtatact ttgctttctt
   121981 tggagttatg acctttggaa tgccgtggta cgtgtcattc attattgcaa tagctctgac
   122041 gggagtcctg ggtattactg ttgagacact ggcttacagg ccgataagag aagcaccgcg
   122101 aatttcaatt cttatttctt ccataggtgc ttcttacctg ttggagaatc tggctatcgt
   122161 gatatatggc ggaaggccga agacctttcc gcagatcccg ttgttcaccg atatggtgca
   122221 attcgggacc gtccatattc agatgctgac ttttatgatc cctgtggtaa cggcggttct
   122281 cctgatgtta ttaatgtacc ttatcaacaa gacaaaaagc ggtatggcca tgagggccgt
   122341 atcaaaggat tttgaaactg cgaagataat gggtattgat gtgaacagga caatatcctt
   122401 tacctttggg ataggatcct tactggcagc ggtaggaggc ataatgtggg gcctcaagtt
   122461 ccccagcata ttgcctttga tgggcgtaat gccgggaatt aaatgcttta ttgcagcagt
   122521 aataggcgga attggaaata tacagggtgc ggtaattggc ggattcatac tcggtatagg
   122581 agaaatcctt ctggtagcat tcctcccgga gctttccggt tacagagatg cattctcctt
   122641 tattctgctt atattaatac tcctatttag gcctaccggc ataatgggag aaaaaatatc
   122701 ggagaaggtg taaatatgaa gaggaatacg atttatacaa ttaccgccat aattgtactg
   122761 tttgtatttg tttatttcgg caataaatat ttagactcat atcttttgag aatactgaac
   122821 ctttgtgcga tttatacggt attgggtctt agtatgaatc taataaacgg ttttaccgga
   122881 ttgttctctt tggggcacgc aggctttatg gccgtgggcg cttatactac ggcaatgctg
   122941 acattgccta ttgccaccaa gcagatgaat ttcttcctgg cgccccttgt ttcacctttg
   123001 gacaaaattg tgtggccttt tctgccgtca cttttgctgg caggtatact atctgcattt
   123061 gtggctttcc ttataggagc accggtattg aggcttaagg gggattatct tgccatagct
   123121 actctgggtt tttcagagat tataagagta gtattcacaa acacacagac aataaccaac
   123181 ggtgctttgg gactaaaggg aattcccgct actacaaatc tctggtggag ctttgggaca
   123241 gcggtgataa ccgtttttat aatggtatca ctgattaaca gcagcttcgg aagagccttc
   123301 aaggcaataa gggaggatga aattgctgca gaaagtatgg gaatcagttt gttcaagcat
   123361 aaggtgcttt cctttgccat aggtgctttc tttgcaggca taggaggagg cttgctgggt
   123421 aatatgatgg gaacaattga cccgttgatg ttcagattct tcatgacata taacatactt
   123481 cttataatag tgctgggagg aatgggaagc atcaccggga caattatatc agcctttata
   123541 ataacaatag cacaagaagt tctgaggttc ctggatgaac ctatatttcc caatgtacct
   123601 gcgctgccgg gcttgagaat ggtcgtattt tcaatacttc taatgcttgt agttctgttc
   123661 ttcaggcagg gaatcatggg aaccagggaa ttcagctggg acaagttctt cagattcttt
   123721 acaaaaaggc ctctttcaaa aggaggtgcc aaatgatgaa tgtattgaga acagataata
   123781 taacaatgca gttcggagga ttaactgctg tcaaggagtt caatcttgag cttaacaaag
   123841 gggagatagt ggcacttatc gggcctaacg gcgccggcaa aaccacagct tttaatatga
   123901 ttaccggagt ttatacacca accaaaggga aggtgttttt aaaccaaaag agcactgttg
   123961 acgggaaaga agagcataag gagagagaca taacaggcct caggcctgac cagataacaa
   124021 aactgggtat tgccagaacc tttcagaaca taaggctttt taaggattta agcgtacttg
   124081 acaacgtgct tattgccaac caccttcgca tcgactctaa tgttttcgaa gcgatattca
   124141 aactgccaag atataagaga gaagagaagc agatggtgga aacatcaatg agcctgctgg
   124201 aaaaagtggg cctggcagac agcaggtatg agaaatccag ttcccttcca tacggacagc
   124261 agaggagact ggagattgca agagcacttg caacagatcc gagtatactg cttctggatg
   124321 aacctgcggc aggaatgaat ccaaaggaaa gtgaagactt gactgtattt ataaaagaga
   124381 tcagagatga atttgatttg acggtattcc tgatagagca tcatatgcag gttgtaatgg
   124441 gtatctcaga caggatttat gttctggact acggaatcac aatagctcaa ggaaacccct
   124501 atgagatcca gaataatcaa agggtaatag tagcgtatct gggggtggat gaagatgctt
   124561 aaaatagaca atctggttgt cgcatacggc ggaattgaag ccttgaaggg aatcagcctt
   124621 gaagttgagg aagggaagat aataacactt ataggggcga acggtgccgg caagagtacc
   124681 accctgagat ccataatggg gcttgtgaag cctaaacagg gtgaaataac ctatatgggg
   124741 aaaaacctgc tcagcatgaa gactcaggaa atggtaagac atgggataac ccttgtaccc
   124801 gaaggaagaa gaattttccc aaacctttcg gtgcttgaaa acctcagggt aggagctttt
   124861 gcaagaaatg acgaaaaggg aataaaagaa gatattgaga tggtgtatgg cttgtttcct
   124921 atacttaagg agagaagctg gcagacagca ggcacattgt ccggaggcga gcagcagatg
   124981 cttgcggtag gcagggcgct gatgtcaagg ccgaagctta tgatgatgga tgagccgtca
   125041 ttggggcttg caccgcttat agtcaaggaa atattcaaca tcataaagga agtaaacaag
   125101 cagggagtta caatcctcct tattgaacag aatgcaaatg cagctctcca tattgccgat
   125161 ttgggatatg ttatggagac agggagaata accctgaagg ggacaggtgc ggaattgctt
   125221 gcaaatcctg atatcaagaa agcatatctg ggagcggaag cccattaaaa gaaacgaagg
   125281 ggtatcgata gcgatacccc ttctcatatg cacgggacac ggggacgttt cacgggacac
   125341 ggggacgttt ctcttgtccc actttcaatg ggacaagaga aacgtccccg tgtcccgaac
   125401 gtccccgtgt ccctctccgt gtcctttcgg cttctctatc ctctatttcg gtttatgtat
   125461 acttcagtat cacccttttt tactgccttc aattcggaag taaagagctt gttttccggg
   125521 tgctgctcca ttatatcaag tacctctatc agtttggtca agtattctct gatactcaat
   125581 tcggctaatt tcccgccgaa ctttcttaag gaaatgaagg tccagcttct gatggattca
   125641 ttgctgtttg cgggtacaaa attataaagc attttatata tattaattac tctgtcggta
   125701 agctcctgaa attcttcggt attaagtttg tgaagcctta ttacgggctg ccttacatca
   125761 tacaaagcgg aattggattt gtctatggca tctcccagtc tttgggacag agcctcataa
   125821 gtttttatac ctttttcggg attctcaaag aagtcattgg tggcggcaaa aacaaacatg
   125881 cagcttccca attcccccga gcagcataca tcaataatat ttctgagatt ttcataggaa
   125941 actcttctga tatctattct ttcgttcata atcagctcaa gctcgtctat aaggattata
   126001 aggccgctgt atcccagcag tttgatcagc ttcacaaaag ccttcaggaa gtcaatagaa
   126061 ttaagcctgt caacgctgcc taccacatca aattttgctt taatacttgc gggaatgttc
   126121 ttttctccca ttagccagga ggctactgtg tccgataata ccgtgttctt ttcgattctt
   126181 gcccttatgt atgccagaaa agccctggca aaggacatat tataccggtt aagggatgaa
   126241 ataacatggt ttatttccgc agcagcactg tctttgttgg atgactcctg aagcttggag
   126301 atccaaaggt caaatatgct ctcaaagctt gcggcggagc cttcggctat attaagagcg
   126361 agattatgca taatatgata gtataagtgt tcaagattat tgaatctgaa tcctctgtcc
   126421 acctgtacct tagacacgat aaaatcgttt ttgacggcag cttgcttgat tacattcata
   126481 aaaaaggact ttcccgagcc gtattccccg ttaagaaact tcacagtacc gctgccttcg
   126541 gaaaccactt ggaagcacct ttgaaattcc ttaatctccc tatctcttcc tacacagagg
   126601 agttccaggt cgcagtcagg gacaacgccg tttttcagtg catttattat gtttcttgct
   126661 ctttgtacag taagttccat gacgtgctcc tcttgagtta ttatgctaag tcagtaaatt
   126721 cgacataatg aaacaatttc cttccggaac atgaataact attttcccgc agcttgactt
   126781 tcacaagccg ctttactata ataagtacga aacgaagaaa ggtaaggtat taaaagcatg
   126841 aggatattat tctaattagg aacaactaaa catttagcgg atttatcccc gcaccggggg
   126901 ttattctgct attcattcag ttccggttgg gatacatagt cctatgtata ttataaataa
   126961 cagtatgttt gctgctatag gcggttattg cttcaggcat gctttctgca gctatgtatc
   127021 ctctccggaa aagcaggagg ggaattttta ttgtattaac aagtgtaatg cttatacaat
   127081 agagatgcaa gggggaaatg attatgctct tattggagat taacggcctg aagaaatatt
   127141 acgttgaaag gctaatattg gatattaagg actttaaggc atattacgga gaaagaatcg
   127201 gtatagtagg ggcaaacggt gcaggcaaga caacgctcct tgatataatc gcaggaagga
   127261 atattcccga ggaaggcagt atcaagcttt atggagagat atcatatata acgcagcttg
   127321 atttggaggc ggcctcagag gttgacaacc gtatggcaaa ggaatttgga ttgaatttca
   127381 acagccttga tacggcaagc ggaggagaaa agacaagatt caagattgcc ggcagccttt
   127441 cggaaaacaa cagcatactg ctagctgacg agcctgcttc aaacttggat atacagggga
   127501 tagaacttct tgaaaaaaaa ctggcaagct ataaaggctt gatattaatc gtatcccatg
   127561 acagagagct tttggataag ctttgcagca agatagttga gattgaggga ggagaattga
   127621 agcagtacag cggcaattac tcccaatata agctgcagaa ggagatggaa ttcaaaaggg
   127681 ctcaatttga atatgatcag tatattgaca gcaaaagggc attggaagag actgtcaaag
   127741 aaaagaagaa tagggcggcc tccatgaaga aagcaccttc ccgcatggga aactcggagg
   127801 cgcgtcttca taaaacggga aaccagaagg ctaaagcgaa tcttgacaaa gcaatcaaag
   127861 ccatggagac cagaatcaga aagcttgaga aaaaggaaaa accgaaggat cagcagcacg
   127921 ctattatcga tattcagggt gccgaaaaac ctgtaagcag agtactgata tcgggtagcg
   127981 gcgtatcaaa atcctttggc tcgaaggaat tgttcaggga agcggagttt gaagttctga
   128041 acggatccaa gactgccctg ttagggggaa atggcacggg caaatctact cttctcaata
   128101 tgataatggg gggagatgag tcaattaaac ttgccaatgg tgtaaaactt gggtatttca
   128161 gtcagggaac ggatatttta aaggatgatg aaacaatact tgaaaatgtg atgaaagaaa
   128221 gcatatatcc tgaacacttt gtaaggacga tacttgcaag gctgctcttc agaagggatg
   128281 atgtgtataa aaaggcagga aagcttagcg ggggggaaag ggtacgaacc tgcttcgcaa
   128341 aaatattctg ctcggatgca aatgtcatta tccttgacga gcccaccaac tatcttgaca
   128401 taagctctat ggaagcggtg gaaaatgcaa tgaggcttca tgagggcact atcttttttg
   128461 catcccatga caggaagctt atcagcagca ccgccgacag gataatatac attgagggcc
   128521 gcagactgaa tacctttgaa ggaagctacg gtgaatatgt tgaattcagg aggcgtgcag
   128581 cggattccgg tgaaactgaa aggaagagac agaagctgtt gcttgatacc aggctttcgg
   128641 agatattaag caggctgtcc atgcccggaa aggatgatga cgtagaacag ctcgacatgg
   128701 aatataaaag ggtactggct gaaataagac agctgaagtc cggcagataa cttgtgcata
   128761 agcagggaaa gctgttgcag tattggttgc tgacaaaata tgattatatc ggaggttgaa
   128821 cagatgaaaa aagtaatctt agacgcgtta aaggaatccg ggacggattt atccgagata
   128881 gatgttattg taggcagggg agggaatatg aagcccgtac aaggaggcgt ttatgagata
   128941 aatgaagcaa tggtggaaga tttaaggctc ggtgtaatgg ggcagcatgc gtcaaaccta
   129001 ggaggtataa tagcttactt tatagcagcg gagttaggca tccattcata tgttgtcgat
   129061 cctgtggttg ttgatgaact tgaggaatat gcaaggattt cgggaatacc ggatatcgag
   129121 agaaaatcca aggatcatcc ccttaatcag aaggctgccg ccaggatggc agcaaaagaa
   129181 ctgggaggaa attacacgga tttcaatttt gttgttgccc atttaggcgg cggtatctcg
   129241 gtaggggcac acaaaaaggc agaatggtag atgtcaataa taccttggac ggagacggcc
   129301 ctttctctcc tgaaagatcg ggcggaatag cccatgatac ggagtttgtt tcatggataa
   129361 aagaaagaat ggcatttatt gctccggtta aggtatatcc gggagagact gaaatgaaag
   129421 cactggttca gggagtgtta agggtattaa agggcgagga gcagctaaaa tcatataatt
   129481 gaaaaacacg aagaggcaag aaaatatttc tgcaatcgaa aattgtctgc tgatgcgata
   129541 taattaactt agattttgtc ttacggctgg aggtaatagc ttggatgtag cagctgcact
   129601 tattgtcaca tttatgcttc tcatgcttag cgtatacagg ggaataaaca ttttatatcc
   129661 tctccttata gggctgataa tatttattac agttgcatat aaaagaggtt ataagccaaa
   129721 agaaattatc ggcatgattt taaaaggtat aaggaagtcg atgcttataa taagcataat
   129781 gatgcttata ggtgccatta tgtctgtttg gagagcttcc ggaactgttg catatgtagt
   129841 atattacggg ataaggttcg ttgatccgaa tctttttata ctatatgctt tcttgcttag
   129901 ctgtgttgtt tcttttttgc tgggtacttc ctttggaacg gttggtacta taggcacgat
   129961 acttatggta atggtaaagg gcggggatat aaatatcaat attgttgcag gtgcgattat
   130021 gtcaggcata tactttggag acagatgttc gcccatgtct tccagtgcaa acctggtatc
   130081 tgttcttaca gagacggacc tttatacgaa tattaaaaac atgttcagaa ccactgcagt
   130141 acccttcctg attgcttcaa ttatatacgg gatactgtca tttcagaatc ctctgcagct
   130201 tggagaaaac cggataataa cagggatagt acagacatac aatttgaatt gggtagtgtc
   130261 tatgccggcg ataataatac tggtactggc ctttttcaag gtagatataa aaatctccat
   130321 ggctgtaagt atattgtgcg gggctgctgt tgcggttttt tttcaaaagg agaccatagg
   130381 ggatgtgctg aaatatatta ttatgggcta cagcaggccg gagcaaagct ttttgggaga
   130441 tataatggcc ggaggcggta ttatctcaat gattaatgtg gtactgattg tactgatagc
   130501 cttttcatta tccggagtgc ttgaaggcgc atccttgctg aaatttgccg ggaacctgat
   130561 tataaaactc gggaaaaaag tcggcatatt tgctgctgtt attctaacca gtataatagc
   130621 gggggctttc ggcggcagcc aaaccttagc cgtgatgctg acatatcaat tggttagaaa
   130681 tatgtatgat tccgcaggta tagacaaata tgagcttgca gtggatcttg aaaataccgc
   130741 aatagtgatg gcgcctctta tcccgtggaa tattgccggg gcggtacctg ctgccgcatt
   130801 aactgcagac aacagttatg tcttgtattc gttttattta tatttaatac ctttagcaaa
   130861 tctggttatg aaaaaattcg agataattaa acgacataaa atggcttaat ttattttttg
   130921 aacatgggag aacatataat ggatgttata ttaaacgcgc ttcaatctgt attaagtatg
   130981 gcagtgctga ctatgctggg atatttcctg acacttgtta tttttattcc aagcttaatg
   131041 accaaggtgt ttgtaataca atccgctatg cccgttatcg cacagtctgc aatagtagct
   131101 gctgcttttg gcggggatca atattatgca acatatatgg ttacaataac ctcaattctg
   131161 agcgtggttt tcatccctgc gtatatggta ttattgaacg gggtataaat aagttgttat
   131221 agaaaggttg attttattga tttcaagagt caagcgaaaa agcacaattt ttataattac
   131281 agcgttattt atattgattg ccggaggctg ttccgtaaag caggagagtc cgaacatacc
   131341 ggttcctgct gttgtgcctt ctccgacagc taagggaacg gatgtcaatc agtatattat
   131401 agatgcggtt cttgatacgg ctaataagga gctgacagca gagcagcagg ttatatatgt
   131461 gaataatgat gatgtggaac tttcagagct ttattttcat atatacgcca atgcattcag
   131521 gaagcaagag acagctccgt tcctctttga tgatttttcc atggcctatt ccaggggttt
   131581 taagccggga tatacagaaa tcgaggccat tgagttttca aaggggcaga atcggaacgc
   131641 ccttgaatac agccttcagg gagtcggaga aacaatactc agaatcaagc tccctgagcc
   131701 tctgaagccg gagggcagtg taaatctccg gtttaagtat aaggttatca ttccccctgc
   131761 gggagaaagg ttcggctatg gagatgccaa tataaacatg gggaattggt atccggtggc
   131821 ggctgtattt gatgatgaag gatggaatct ggataaatat tactctatag gagatccctt
   131881 ctacagtgac gtttcggatt atattgtgag tataaaggca cctgaggaat atatcatagc
   131941 agcttccggt tccttggcgg aagaaagaat agaaggcgga tataaaaatt ggaagttcca
   132001 tggagccaat atgagggatt ttgcatttgt cgcctccaat aatttccggg tagctgagga
   132061 taaggtcgga aatactatag tcaagtcata ctactataaa gatcatgaaa agagaggaaa
   132121 ggaagttctg gacattggga agaggtccat agaggttttc aacagcgcct atggtgagta
   132181 cccctatccg acatactcga ttgttgagac ggaatttccc agcggtatgg agtatcccgg
   132241 acttgtatat ataagcacaa agtattacga taatgattcc ggtgcggata tttttatgta
   132301 tactacggtt catgagacag ctcaccagtg gtggtatggt gttgtcggca acgaccagat
   132361 tgatgaagca tggctggatg aaggttttgc cacctactcc gagggtgttt ttacagaaat
   132421 ggaatatggt gagggtaatg gagatctgta ttataaatac ctggaggaaa gcgcaaagga
   132481 aagtatcaga gcaaaagctt atgacggtgt gatacttaag cctctgagca gatatgagaa
   132541 ttgggatgac tacggtcctg cggtatatac aggcggagca gtgctcttga atgaaattcg
   132601 aaagcaggta ggagaccacg ccttctttat tatactgcag acctattatg atgaatattc
   132661 cttcaaaatt gcaactaccg aagacttttt aagaatatgc gagaatgttg gcaatgctga
   132721 atttgacgat cttttccata agcatctcag cagtgtgaaa tagaagaatg cgtcacattc
   132781 tccgggtacg aggttcgagg cacgagagtt gaggtagggc ttcgaaggaa gtgctgtcat
   132841 actgagggtc gctaagtatc ttgacccaag attcccagca ggcttgaaat gacaagagcc
   132901 ctgtggattt tgccagatcc gcaggactct ttttgaagta aaaagtatag agcataggtc
   132961 tatacttata acgttgcgaa aagatgtctc ccaacaggtc atgagcatat ctcccgcctc
   133021 gtttattgaa atcttgttat aaatttccga aatatattag actttttccg gcggtattcg
   133081 tagtatacta tagaaatcgc aaaaatgaaa agtaatttat tgattgcaaa ataaatagat
   133141 cggaggatag gttgaaatga taaaaaagaa ggtaggcagc ataaccctgg ccgtaggact
   133201 tattactgtg ggtgcactgc tgtttgcaca aaattttatg gatatacccg taaaggacat
   133261 atataaatac tggccggtgc ttttaatcgg attaggtttg gaaatgatac tttatatggt
   133321 actgtacgga aggaacaaaa cagaagtcaa gctcagcgtt gatgggttgt gcatagtctt
   133381 cataataatt ctcggaatta tatccaatgg aatcagattc atcgatatta atattccggg
   133441 gcgccatttc ttcggattca acggaagctc catgtttgaa ggctttaagt acaggacgga
   133501 gatcagcgaa aaatatagca aggataatat atccggcggc tttaatgtta agacccttaa
   133561 agtaaccaat gatttcggcg aaatcaagat tctgcctgcc ggtggaaata ccatcaggct
   133621 ggaagcggag gtaatggtta agtgcaatga tgagaacaag gcaagggaat atgcaagaaa
   133681 tgccgttgaa ataattgagg gggaagtcac ggagctttct cccaaaatcc cgcccaactg
   133741 gaataagaat gatcatgcaa gagcgcaggt tgactttgtg gtatacgtac ctaaggaaat
   133801 aagcctgata gcccgtggga gctttggaga tatcgaagcg gaaggaatag ggggagaatg
   133861 cagaattgag aacaaaaatg gtgaaataac agcttcaaac ataggcggag atgtggatat
   133921 tgacaattcc ttcggagata tcgaagttag caatatagga ggaaaggcgg atatatccaa
   133981 ccataacggg gagatatcag cagacaatat cggcggttct gctgtaattg aaaatcattt
   134041 tggggatatt gaagcggaaa aagtcggcgg agatttgaag gtagtaaaca acaacggaag
   134101 gatatacgtc aaagaagttg gaggcaaagc tgacctggtt aatgcttttg gagacatagg
   134161 cataaaaagg gccgacggcg gcattactgt ggaaaacaag aacggaagga ttgaagcgga
   134221 ggatgtagcc ggaaatgcgc agataaagaa tgccttcggg gatatttatt acaaatctgc
   134281 aagtgcagat aacggagata tatatgccag gacaaaattc ggaagcatta attgcaataa
   134341 gccgctgcag attaaaaagg aagggcagga taccgtcgca cagggaaggc tgggaacggg
   134401 gcaatacaaa atagagcttg aaactaataa tgggagtatt gttatagaat aaggctcaaa
   134461 gaggacacgg ggagggacat ggggannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   134521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   134581 nnnnngggga gggacatggg gacgtttccc ctgtcccgct ttcagcgaga cacgcgaaac
   134641 gtccccgtgt ccctccccat gtcctagtcg cattattttt ctattatgct ataataagtt
   134701 tagtttgagt aatatattga taataataga tacggagggc atatgtataa ggatataaag
   134761 gctgtaatat ttgatgtgga cggtaccctg atcgattcca tgtggatatg gaagcaggtg
   134821 gatatagaat tcctggggaa aaggggtatt ccgcttcccg aaaggctgca aatggagata
   134881 gaaggaatga gctattccga aactgcggtt tatttcaagg aaaggttcaa ccttcctgaa
   134941 tccttggagg agataaagga agaatggcgg cttatggctg aggattatta caaatgccat
   135001 atccagttga aaagcggggc aaaggagttc ttaaagctgc tttatgataa gggcttgaca
   135061 ttaggaatag ctaccagcaa ttccagggag cttgtagact gtatgcttgc taatcatggg
   135121 ataagaaagt acttttccaa tatcagaacc tcctgtgagg ttgacaaagg aaagccgtat
   135181 cccgatgtat acctgaaggt ggctgaggat atggcaatcg atccatgccg atgccttgta
   135241 ttcgaggata ctctttcagg ggtgatggca gcaaagtcgg cgggaatgag agtgtttgcc
   135301 atggcggatg aaatatccct ggagtcgaag gataaactga tagagttggc ggaaggatat
   135361 atccacagtt tcaatgaagt aatggagtat tttgcatgaa agaacaataa cttgttcttt
   135421 ttttgcgcct ttgaagtatg gatagaagct gattcgtatt aatacataga tgggcaggta
   135481 aaggaaggtg atgggtattg agggatgaag aagtggtttc gtacatagta aaaagcaaga
   135541 ctgagttatt ctccgtgata attgacagat atcaatcaaa ggtattttcc accgcctttc
   135601 actataccca tgattacgag gaagcaaggg atttgacaca ggaaatattc ataaagcttt
   135661 acaacaacct gcagaaatac aagggcaaag cctctttctc cacctggctt tacagaattg
   135721 cagtgaaccg atgtatcgac tgggcaagga aaaagaagcc gcaaacagta tcagccatct
   135781 gtgacggcag cgaagaggag ctggatatat atgatactat agcagacagc ggcggagggc
   135841 ctgaagaaga cctgataaag caggagaatg cagtatattt gagaaagctt gtggaaagcc
   135901 ttccggagat ttataagacc gtaattatac tttattattt tgaagacttc agtccccagg
   135961 aaatatctga aataaccggt gttcccaagc gtaccataga taccaggctg ttcaggggaa
   136021 agaacctgtt gaagcttaaa atggaagagt tgatttgtgg aggtgaacaa aatgtgctgc
   136081 gatgaaaata gaggaatgct tcatgaatat gcagcgggaa agcttagcga actggaaagc
   136141 tatgctgtgg caaggcatct taagtcctgt gacagatgca gaagagaact ggcggaaatt
   136201 aaggcgctga aagtattcct gaagtcaggt tttcaggaaa gtatcatacc atccgtggat
   136261 ttgaaatcct ccattatggc ctctgtagat ctcaaaaggt acaaaaaatc atacaggaat
   136321 accctggggg aaatggccaa cttgggaatg agccttgttg cagcaggttt gatactcttg
   136381 cttctgaata cagttccaaa agatgatctg gtgcgcgtac agaatgagct gtataatacc
   136441 ggggaaaaca tcacgcaaaa gatatctcaa cccttcagct caataaatga gcgaatagac
   136501 aagctgacaa acagtattac tcagctggac gggattatgg gcaggataga aagagaaaaa
   136561 gagggaggaa aataaaatga attgtaaata tcataaagaa tcggaagcga aattcatctg
   136621 cgataaatgc aaacagccga tttgcgagga atgctccgtt gatgtaaacg gaaataagat
   136681 atgcagcagc tgcattcaaa agtcgctgtt ctcagaaaac aggcagtatc aaaagcccgg
   136741 cttcttcgaa aatcttgcat ttttctgttt tgctgtagtt ccaggagcag cccaaatgta
   136801 tatgggcctt ttcaagagag gctttcagct gatgttcggt ttcatttcgg ctattgtatt
   136861 gttcagttac ttgaatactg aaggcatgat aatgcttatc ataataccta cctggttttt
   136921 cagcttcttc gacagctatg ccatgagaag aaggattaaa aaaggagaag aagtagaaga
   136981 cagtgttata tttgattata acatacttat agagaataaa aggattgtag gtatggtaat
   137041 gctgttcctg ggaattctgg gagtagtaaa tgcttttgga cgctcagtct ttcagcttgt
   137101 ctttggagga gatctttatt ggtcaattag aagaagcata atgcctattg cattgatagg
   137161 agcaggagtt tatatgcttt caaaatcaaa gaagaaggaa gaagcaaaaa acactgagga
   137221 cattatagaa attgaagaag aaagtgaaga agaaattgag gccgcagaat aaaatggcag
   137281 acagatttca tgcaagtctg atataatata tattaggatg aaactcgtaa aattaaatat
   137341 tttacgggtt tttttcaaca gacgctgtta gaaacgaggt gaattctatg aacttaatta
   137401 ttgaaaaaga tgctgcagct tatataaaaa aacatactaa agatagctca gtgaccctat
   137461 atatacatgc ggcaggcggc ggctgatgtt caatacaagc tccgacagtt cagttgggga
   137521 agccggacaa agccgaaaac tttgatttat atagtgttga tggagttgac gcatatataa
   137581 aaaagggaac caggatccgc aacgaccaga ttcatatttt tttgagaaag ttcttatgga
   137641 taaaggattt ggcagttgac ggcgtacgtg tcgattacta atagccttat ttatacattg
   137701 acttatcata ggcatttaat tataatgaaa aatgaacttc aaaatcaata atgcgcccgt
   137761 agctcagctg gatagagtgt ctgactacga atcagaaggt cgcaggttcg actcctgtcg
   137821 ggcgcaccat atttgccgat tatcagaaac cttgagaaat caaggttttt tcttgatcag
   137881 ccaatcaatt gcatttatct ggcggatacc atcgtgatta gcatttggca taatgtcatc
   137941 gagggttaat aaaaattttg ggtggtggtc gggtattttt tgcagcggct caagttctct
   138001 ttgtaatgtg tttctatcaa gcaccgatgc ggacacctga tagtattctg tgccttcagt
   138061 attgcctgct acaaaatcaa tttctttttc tgcaagcttt ccgatattta ctttataccc
   138121 cctgcgaata agctcaagat atattacatt ttcaagcaaa tgccctatgt cggctgctga
   138181 acttgataac agcagattac gaagcccggt atcgacaata taatatttac caagagtttt
   138241 cagctgctgt ttccctttaa tatcatatct gtcaacttta taaaacatat agctttggta
   138301 cagggcgcga agataattct caacagtatt aaccgatatt ttacgtccgc ttgcatttat
   138361 agcatcgctg atttttttta cggatactgg gcttcctata ttgctgcata aaaactttac
   138421 aatgctttta agaaccgata tatcttttat tctttcacgg gctgcgacat ccttaatcag
   138481 aattgtgtta taaataccgt caatatatgt tctgacaacg gcatcgcttt tatttaggac
   138541 tgctacataa ggaaatgatc cgaatttcaa gtattcatcg aaagttgatt tgacatctct
   138601 gcccttggca aactcaaggt attctgaaaa tgacagcggc agcatggaga tttctatata
   138661 ccgtcccgat aacagagtag caagttctcc tgacaacatg gttgcatttg agcctgtaat
   138721 atatacatcc acattttctt ttaagaataa gctgtcaacc gctttttcaa aagacttaca
   138781 ctgctgaact tcatctatga agacataggt atattttcct tttactattc ttgctttaat
   138841 ataatcataa agcggcctgt aatccagcag gttttcatat tcgatatctt caaggtttat
   138901 caatattatc tgttcttcgg atacgccggt ttttttcaaa tagtcaatgt acaaggaaaa
   138961 caaggtagac ttgccgcatc ttcttacacc cgtaacgacc tttatgacct gcttttcacg
   139021 ccactctata agcttattta agtattcttt tctttgtatc attataaaca cctctcttaa
   139081 aatgagtata acaaaaaaat accgaaaagc aaagtttttt ctgtaatcag aaaaaagtat
   139141 cataattcga caagtatttt ctgataacac atacctttgg tcaggctgtt tattcaagct
   139201 aatagatgct tttgcaggat atatcagaca tttctagaat tatatcgtat cagttattga
   139261 atactttaga aaggggtact atgatgagca agaaaaatta taaaatgatc ggcatactga
   139321 tttgcattgc agcaatcgga ttcggggtct tttattttat caatattgca ccggccaata
   139381 gggttttgta tgaagtaaga aatgctgaaa taaaagatat tgacttgaac gagatcgctg
   139441 acggcaaata taccggggaa ttcagctata ataatacacg ctgcatggtt gaggttatag
   139501 taaataatca caggatagag gcaataaatg tcctggaatg cggtactcct gaatatggta
   139561 agaaagctga ggctgtaatc gatagaatta ttgataagca gaaaaccaat gttgatgcag
   139621 tatcaggggc aactacgaca agcaaagccc tgcttaaggc ggttgaggcg gcgttaagca
   139681 agtaaattta tagatgattg gaccggttta agcagattca ttgttatcgc agatttgctc
   139741 atttgactct tcagttatgc cgctgtcgga ttcttttttc ttttttcctt tgaaccatct
   139801 gactattacc ataacagagg atatgaaagc catagcagaa ccgccccaat aattaatctt
   139861 atcaagaaaa tcaccttgat ttatattttt catcgaacta tcgggtttat cgtaaacagt
   139921 gaagtaaaat gaacggctta ttgggtatct gtcagtaagt atggtttctc tgctacttgt
   139981 tcaacagtaa gatttgtcac cggatttgaa gggtggactc tctatcgagg gcgttaagag
   140041 gcttaaaaag cagtgggtat ataatctaaa cagcaggaaa gcaaaccaaa ataaatacaa
   140101 aaaaagagaa gaaa
//
LOCUS       DAOOAB010000002        85469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_301_length_85469_cov_16.415049, whole genome shotgun sequence.
ACCESSION   DAOOAB010000002 DAOOAB010000000
VERSION     DAOOAB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 85469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 85469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85469
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_301_length_85469_cov_16.415049"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2325)
                     /locus_tag="PKU88_00725"
     CDS             complement(106..2325)
                     /locus_tag="PKU88_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX67845.1"
                     /translation="MKVKIKLLLLALVYMTTTLIPLTVHAQVHQSPQIQWQKAFGGSD
                     NTEISEVIQTSDGGYLAIGTTNSPDGDITTKQRVNEPGSGFREDDDLLVVKFDSNGQP
                     VWHQTLGGSAGDMGFAVRQHSDGTFILAGMTFSKNGDVVSNQNNPSSDFVVWMVKLSA
                     KGDILWEKVLQDVDTEASKVISLHIIDDGYLMGIQGGKNNFYNAYAVRLDFQGSIRWQ
                     KTLPGYYLYDIAQLNNGDFISAGDFIVRFTQDGDILWQEPSVKEEQSIEGTADGGFVW
                     AVSVNQYHRELIDLYKSVTKRSFPMALYSDTYNTSPIIMVVKFDSGKNPQWANYLGGS
                     RNEHVISIKQTSDGYYAILARSESRDGDISDSKGNSDFWLTGLSSEGILIWEKSLGGS
                     NADEPKSLVAEKDGYIVAGYTNSNDKDVKGLHNPTKSYSYDAWIAKLGWNEKLDTYAG
                     NYPSSWAQTDVEEAKTLGLTSNNLLGSYTENITREDFSELVVRLYEILTKHEAPLPEA
                     NPFNDTVNLYVLKACELGIVTGAGNNMFMPKDSLTREQASLMFFRLMNKIKPGLDTHV
                     SSPKMFEDHTNISNWAVDAVYYINYRGIIQGSNNQMTPKGALTREQAIILAKRMYENI
                     DKYQLDEFRLVQQSPIIVSAGQTININVIVRGSDFSNSEKITTWKVDDNSIASISGDI
                     VGGYTNWILGEVAEKTVIISGYSASVTGNKEGTTRLTVFIGERSQVQSRTYEIIVKR"
     gene            complement(2362..3882)
                     /locus_tag="PKU88_00730"
     CDS             complement(2362..3882)
                     /locus_tag="PKU88_00730"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HPX67846.1"
                     /translation="MKCINLFIRYKHWGKIVFTLAMSMIIILGGLLEVYAITFSGSLP
                     IGSYVQFGMYKDKPIVWMVVHNDENGSILFAKDIITVKAFDASGDRAEGRDSKYNTRV
                     AEGSNYWTKANLCEWLNSEEQTVNYSHQNPDAANTMGVSYSTFVNGKSSILAEDVSYA
                     DEPGFLTNFHDMEMAAIQTISLRTYLSEFDLPVKDGGNQPWNLNGDNCNAYYKLSSHK
                     IFLLSVDEVKAWIEENELNRTAPWYTAPNGWQINTYWLRTPDYDYAASVMTIPSNKLS
                     DPHGFNAYQANGVRPALCLKPGYSMSGPGTEYQPYVIRYEVPEEDAEGDISDKPSFWA
                     EEEVTRAIRNNLVDESLLSQYQNDITRQEFCTLAVKLYEELSGRKATYDMVTPFSDTN
                     DISVLKSYNLKIIQGVGNGRFAPNENISREQIAVMFYRILEAILPSRPDIQLKTSHEI
                     RFYDNNEISDWAVKGVTVMNGEKVILGTGTGFNPKGTATREQAFILANRMFEKYHR"
     gene            4115..5590
                     /locus_tag="PKU88_00735"
     CDS             4115..5590
                     /locus_tag="PKU88_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67847.1"
                     /translation="MEFHDKLNFLMDITKTTNISLARASSMDPSYISRLRNGKRKPVK
                     NASYMIVFARFFSKRIHSEKQMDVFREVLLPYTMFPESESALTDFLHHWLVNNDFKNG
                     HLNLPVLHRLTDAEPRGGFNKNLNENVKPFFKTACGLYQGIQERRLAAVHFLSTILNQ
                     NSPRTLFFFSDEDMSWMTGDPVFYQKWSHLMGQIIARGNRIKIIHDLTRGLDEILSSI
                     QLWIPLYLSGAIEPYYYPRKRDGIYKRSLYIAPGLCAVSSDSIGTMTADESTAVFHQD
                     LRMVEALENEFHHYLNLCRPLMSIFTPDKHQNYLSLLESFEREYAPTIFKTPHLSMLS
                     MSESLMESILLRYCKTSYNKNRSFYNKRLENFRKTLKRHSFTEIFSIPEKEALLAGKA
                     RINLPNPFESIEGFYTPDDYRRHLDHVAVLTKHYDNYNVCIEKNECSLGYTLYAKEGI
                     GVIVAKTKTPAVVLFIQESNLFAGFWDLLIEMKEHGARHDV"
     gene            complement(5851..6822)
                     /locus_tag="PKU88_00740"
     CDS             complement(5851..6822)
                     /locus_tag="PKU88_00740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX67848.1"
                     /translation="MFNKKCRLLLVSLALVLLTVFPTACNMVRVNPEVDNKTVVAKVS
                     GKEILKEEFNKMFDIFKTQYEQQFGTEVWDQEVEGRKFGDVAREKLLEMLIDEKLQME
                     KAAELGITVTDDEVNAEIENARKYFDSEEKLNEFLKGQGIDLEYFKESVKKELTINKL
                     VEKLTEDITVTDEELKNYYDTHQDEFMNVKASHILLDTKEEAEKMLTRVKAGENFAEL
                     AKQYSKDPSAKENSGDLDYFRHGDMVEPFEKAAFALKPGEISEIVQTDFGFHIIKVED
                     SKRDKFEDVKEMLKGKLLYEKKGSEYEKLLEEMRKNANIEKFVKNLK"
     gene            complement(6873..10391)
                     /gene="mfd"
                     /locus_tag="PKU88_00745"
     CDS             complement(6873..10391)
                     /gene="mfd"
                     /locus_tag="PKU88_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPX67849.1"
                     /translation="MSIGNVLKPLYEMEEFNELLSSLEANLNTVEVYGISDTQKAAAA
                     AFVCRHFGKACLFITYNDLLSRKIYEDISFFEPDMAALMPGRELVFHRIDARSNEILQ
                     NRLRAYDDIISGKNCIVCTSIEALLTRTVSPELFASMTRELSVGQTVSLDEIKEYFIK
                     AGYERIEMVEGKGQFSIRGGIIDFFSPIHQNAVRIELFDDEIDSIRYFDVLTQRSISK
                     TKKVKITPAREFIVKEEEFLEGAEKLQSELSARLRASGNERRRKSSDIRVREKISEDI
                     EKMRQGIHFEGIEKYSPYFSDKNYSLIDYMGDCVIFIDEPNRIKQRCEAIGTEFMEHF
                     NQLLEKGEVLPGQFEALFTYEDVVPKIREKKTVCLDSLYKSSSDFIPQKTISITSRSM
                     HPFHGKLSMLMEEIAVWRKNRYRVLILSGSRDRGLRLASDLRDRDIDAVYKDELDIEL
                     KEGHVIILPGILNSGFDFPSIKYAVISDREPFGSKSRKKIEKKKGKAINVFTDLKAGD
                     FVVHENHGIGQYIGIEKLKIDNTTRDYLHIRYSGNDKLYIPTDQLDMIQKYIGAEDKG
                     PKLNKLGGTEWVKVKAKAKKAIEAMAMDLLKLYAERQQAVGFEFSEDTRWQKEFEDMF
                     QYQETQDQLRCIEEIKKNMEDSRVMDRLLCGDVGYGKTEVALRAIFKCVMDNKQAAIL
                     VPTTILAQQHYNTCIQRFANFPVNIDVLSRFKTHAEQSRIIQNIKNGNVDIIIGTHRL
                     LQNDVEFRDLGLLIVDEEQRFGVAHKEKIKSLKKNVDVLTLTATPIPRTLHMSLSGIR
                     DISIIEEPPEERYPVQTYVMEYNEEVIKDAIAKEIARGGQVYYLHNRVRSIQKIAAKL
                     KAMLPDARVAVAHGQMEEKMLEDTMLDFYNNEYDVLVCTTIIEAGLDIPNVNTIIISD
                     ADKMGLSQLYQLRGRVGRSNRLAYAYLTYQKDKVLNEVAEKRLQAIREFTEFGSGFKI
                     AMRDLEIRGAGSLLGREQHGHLEAIGYDLYIKLLEDTVKELKGEEVTETVDTSIELKI
                     SAFIPESYISDENQKIEIYKKIAYIGSQEDIYDIEEEIEDRFGDLPQVVRNLLDISYI
                     KHLAGKCNVVSITQKKNSILVKFSSDRSIKPETAIRVAGEYIDRLLFTASGQPYFTIK
                     VDEDRPDEYIKFLKEFLGKISSFQNG"
     gene            complement(10457..11023)
                     /gene="pth"
                     /locus_tag="PKU88_00750"
     CDS             complement(10457..11023)
                     /gene="pth"
                     /locus_tag="PKU88_00750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPX67850.1"
                     /translation="MYLIAGLGNPGDKYKYTRHNVGFMVLDYFASVHNISISRIKHKA
                     LLGEGMIGEEKLVLAKPQTFMNLSGESIYELVNWYKCDISKLIVVYDDVDIDIGRIRI
                     RTEGSSGTHNGMKSIIYLLQTDQFPRIRVGIGKQPDFMDLADYVLSRFSDEEIPLMEE
                     AVKNSSLAIEEIIKNGINSAMNWYNREK"
     gene            complement(11057..12004)
                     /locus_tag="PKU88_00755"
     CDS             complement(11057..12004)
                     /locus_tag="PKU88_00755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPX67851.1"
                     /translation="MNVHGKSIKVLNCNANKDLAKDIAFNLGIPVGDAQVSTFSNGEI
                     GVSINESIRGADVFVVQSTCTPVNDNLMELLIMIDALKRASAGRINAVMPFYGYARQD
                     RKSKSREPITAKLVANLIEAAGADRVITMDLHAPQIQGFFDIPVDHLYAAPLIVNYFI
                     NKKLDDIVVVSPDVGGVTRARSLATKLDTPLAIIDKRRPKPNVSEVMNIIGDIENKNC
                     IIIDDMVDTAGTMVNAAKVMKEMGARDIYVACTHPVLSGPAFERIRDSVIKELIVTNT
                     IPLLAGKPVDKIKVLSVAPLFAEAIKRIYEGLSLSKLFD"
     gene            complement(12029..13399)
                     /gene="glmU"
                     /locus_tag="PKU88_00760"
     CDS             complement(12029..13399)
                     /gene="glmU"
                     /locus_tag="PKU88_00760"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014522003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HPX67852.1"
                     /translation="MSNVYSITPIILAAGEGKRMYSKVPKVLHKVCGLPMVEHVINCA
                     REVSGAEPVVVIGHGAEQVRKEVTGVKFVMQERQLGTGHAVMQAEKYINDGDILILYG
                     DTPLLRPDTLLEMREMHRAEGCSATILTSDMDDPAGYGRIIRIGEKLIEAIVEDKDAD
                     SSTKLIKEINSGIYFFDGKELQEALGYLKNDNTQGEYYITDVIGIMRKKGLKIGAFKI
                     KNAEEILGVNNRYQLSEANKIMQRRIVKKLMLEGVTIIDPDSTYIDASVTVEKDVTIY
                     PGCIIEGNTVIQEDSVIGPYSRIKDAKIDRGVKIQNSVVLESSIGEGTSIGPFAYLRP
                     GNKIGKHAKVGDFVEMKNSTFGDNSKASHLTYVGDGDVGSNVNLGCGVVFVNYDGKNK
                     YRTYVGDNSFVGCNVNLVAPVRVNENTYVAAGTTVTKDVPQDSLAIGRARQENKEGWV
                     TKRKNK"
     gene            complement(13540..14379)
                     /gene="nadC"
                     /locus_tag="PKU88_00765"
     CDS             complement(13540..14379)
                     /gene="nadC"
                     /locus_tag="PKU88_00765"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPX67853.1"
                     /translation="MDNIIIDRIIKNALREDLGWGDVTTNSTIPDTALIRGNFIAKEE
                     GIICGIEVCRRVFEMVDGTIDFQARMKDGQRAFKGDIIAEISGNARSILKGERTALNF
                     FQRMSGIATMTSKYVSEISGLKTRIADTRKTAPGLRILDKYSVKAGGGFNHRFNLSDM
                     VLIKDNHIKAAGGITPAVAAARAKGSHGLKIEVEVESIRELIEAIEAGADIVMLDNMT
                     LDMMKEAVSIASGKVLLEASGNITIGGERSVRAVAETGVDIISAGALTNSVKAMDISL
                     RFL"
     gene            complement(14394..15950)
                     /gene="nadB"
                     /locus_tag="PKU88_00770"
     CDS             complement(14394..15950)
                     /gene="nadB"
                     /locus_tag="PKU88_00770"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPX67854.1"
                     /translation="MPLRYLTSFDTRKIESEKWDVVVIGSGVAGLYSAVNLNPGYKVC
                     VLSKETMDENNSYLAQGGIAAAIGEDDMPEYHFMDTIKAGAGHCNEEAVTVLVEEAPK
                     DIKLLEKLGTNFDREPDGTLTRTREGGHGRFRIVHALGDATGKEVVDSLLRVCRDREN
                     ITIKENCFAIDLLVSEGRCIGVLVATGIDTEATKKVLYCRSVICASGGVGQVYRNTTN
                     SEVVTGDGISMAYRAGAVLSDMEFVQFHPTAFYTPEYTGNRFLISEAVRGEGGILLNK
                     NHERFMYKYNEMGEIAPRDIVSRAIFSEMKKTGSEYVYLDITHKDSDYLKKRFPTIYK
                     SCLEHGVDITKDYIPVSPVQHYFMGGIQTDLLGRTNIKCLHACGEAANTGVHGANRLA
                     SNSLLEGLVFGRRSAEYINAGIKDIKLEEVHVSNVYGASGKTLNVMDIRNEIKTLMDA
                     HAGIERNGPDMEKSLNRINEIIGSLEDAEMGSVSAMETLNIAYIASLILKGAIARKKN
                     CGSHYRTDSE"
     gene            complement(15963..16871)
                     /gene="nadA"
                     /locus_tag="PKU88_00775"
     CDS             complement(15963..16871)
                     /gene="nadA"
                     /locus_tag="PKU88_00775"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPX67855.1"
                     /translation="MQNNLAEEIQRLKKERNAIILAHNYQVPEVQDVADVVGDSFSLS
                     QHAAETDSDVIVFCGVHFMAESAKILSPDKTVLLPVRDAGCPMADMVTAPKLREMKAR
                     YPDAAVVCYVNSSAEVKAESDVCCTSSNALKVVESLENKQVIFVPDKNLGSFVASKVK
                     NKEIILWKGFCITHKRVKVEEVQKIRQLHPNAKILMHPECEPEVQKLADFLGSTSAII
                     KYAGEIPEKDVIIGTEEGILHLLKRQNPDKNFYLLSTGLICTNMKKTRLDDVHSALLN
                     MQHEIHVDEDIRIKALKSLERMLKIK"
     gene            complement(17071..17406)
                     /locus_tag="PKU88_00780"
     CDS             complement(17071..17406)
                     /locus_tag="PKU88_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67856.1"
                     /translation="MTDKKKQYPKAEKPEMDMHDMEIPEMEMPMHGCPMICPMMCPMM
                     HHGEWQGMEDMRMPDWYEEDDSDYSSDESDEYPVYWKHKKKHKYSPYPFYWPPFYPVK
                     PYKKKHKRW"
     gene            complement(17527..17787)
                     /gene="spoVG"
                     /locus_tag="PKU88_00785"
     CDS             complement(17527..17787)
                     /gene="spoVG"
                     /locus_tag="PKU88_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HPX67857.1"
                     /translation="MEITDVRIRKVNAEGKMKAVVSVTFDNEFVVHDIKVIEGQEGLF
                     IAMPSRKTPDGEFKDIAHPINSSTRERLQTSILEEYGKVKNE"
     gene            18247..19626
                     /gene="murC"
                     /locus_tag="PKU88_00790"
     CDS             18247..19626
                     /gene="murC"
                     /locus_tag="PKU88_00790"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPX67858.1"
                     /translation="MFSLNNVKNVHFVGIGGISMSGLAQILLESGYNVTGSDINDSNL
                     INKLRKNGAEVTIPHNPKNVETAELVVYTAAVKQDNCEIIRAKELGIPVIDRATFLGM
                     IMKNYNYGIAVAGCHGKTTTTSMISIIIKNAGLDPTALIGGEIEAIDGNVRVGKSEYF
                     ITEACEYCESFLKFYPFVAVILNIERDHLDYYKDLEHIISAFRKFAKLVPADGALVVC
                     SDNENTLNVSKDVECKVLTYGIKNKADFYARKIKYDKLGHPSFEVMHRDKKLGTFSLN
                     IPGEHNVLNALAAIAAAYNAGVSMNIVKKSLKEFKGTYRRFDIKGVRNNITIVDDYAH
                     HPTEIKATLRAAKQFPHQKIWCVFQPHTYTRTRTLFKEFSEAFYDADNVIITDIYAAR
                     EKDTGLVNPSELVDSINKCSANAVYRNDFRCIANLIAHEAKPGDLIFTMGAGDVFKLG
                     LMILDEIKI"
     gene            19771..21363
                     /locus_tag="PKU88_00795"
     CDS             19771..21363
                     /locus_tag="PKU88_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HPX67859.1"
                     /translation="MEESSINSTYHTINHWNNFKLKLVFEGVIVGAFSGLVVVLYRYL
                     IELAGHFSKKIYLMQKGNNLFIVLWLVFLIFLGYFIGVLIKKEPMISGSGIPQVEGVL
                     LRYFSMNWWKILIGKIAGSILSIGAGLSLGNEGPSIQIGAAVAKGYCETFKKKKLEEK
                     FLITSGASAGLAAAFNAPLSGVMFSLEEIHKNFSPVVLTSAMAASITADVISKYFFGM
                     KPIFNFQGISPLPLQSYHYLIILGIILGLLGAVFNYSMLKAKDLYSMQKWLPEHLRPV
                     FPFIIAGVLGLLLPEVLGGGHELIASMVNLSYSLNILMLILVVKFMFTMISSGSGAPG
                     GVLVPLLVIGALIGSIYGNVLSDVFSFNRECVKNLIILAMAGYFTAIVRSPITGIILI
                     IEMTGSFNHLLSVSIVSAIAYVTADILGSKPIYESLLDRMVNKGSSRFKGDSKTKVII
                     ETAVCMGSLIEGKKIKELKLPSECLLVAVKRGEHEIIPKGDTQIHAGDYLITLTNEDN
                     AAEIREKLTAIAEVLSASIYEK"
     gene            complement(21371..21973)
                     /locus_tag="PKU88_00800"
     CDS             complement(21371..21973)
                     /locus_tag="PKU88_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HPX67860.1"
                     /translation="MHVFEYGQKEIEYLKKKDKKLGAAIDRIGMIKREVTPDPFTALV
                     SSIVSQQISSKAADTVWNRLEALVGTITPESIAQAEILEIQACGMSERKAGYIKGAAE
                     AALSGQVDFTALHTLSDEEIIKKLSSLRGVGVWTAEMLLIFSLCRPDVVSFKDLAICR
                     GMMNLYGIKELSKEKFESYRKRYSPYGSVASLYLWALSAE"
     gene            complement(21973..22437)
                     /locus_tag="PKU88_00805"
     CDS             complement(21973..22437)
                     /locus_tag="PKU88_00805"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPX67861.1"
                     /translation="MVYTYFYDYLIGRIGITEKDSKITNVYITDKELSQDMQLCETPL
                     LKEAARQLKSYFAGELKEFSLPLEPSGTDFMKQVWTALCGIPYGKTATYGEIAGKVGR
                     PKAARAVGLANNRNPIPIFIPCHRVIGADGSLTGYAGGLDMKKKLLELEKDK"
     gene            complement(22434..23003)
                     /locus_tag="PKU88_00810"
     CDS             complement(22434..23003)
                     /locus_tag="PKU88_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HPX67862.1"
                     /translation="MKEHFRISDNEKWQAVVNCDKSYDGLFLYGVKTTGIFCRPSCRA
                     KTPVRGNVIFFDNAVNAIDEGFRPCKKCRPDEAVFEPNLELVNKAKDIIEANYDKPID
                     ISHISKQLGVSTNHLTRLFRQNSGLTPKHYTTKLRISKSAELLGQADKNIIEIAYMTG
                     FNSLSNFYKHFKEQVGQTPNEYRKSRGVL"
     gene            complement(23075..23521)
                     /locus_tag="PKU88_00815"
     CDS             complement(23075..23521)
                     /locus_tag="PKU88_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67863.1"
                     /translation="MIEEIRKLFLEYARSLEIDLDFQDFETELNTLPGKYQPPDGALI
                     LALVEGREAGCIALRKISKDICEMKRLYVRDEYRGLGLGKKLVGMIIDEAMNNNYQYM
                     RLDTLPTMKSAQSLYLSFGFYDIEPYVYNPIQGTRFMELKLKKIDW"
     gene            complement(23629..23862)
                     /locus_tag="PKU88_00820"
     CDS             complement(23629..23862)
                     /locus_tag="PKU88_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HPX67864.1"
                     /translation="MKNRDRIKVSKEKRDDMLSAIKYYFLKERDEELGDLAASLILDF
                     IIEELAPEFYNKGVCDSQKYMNDRVEDLLSIQI"
     gene            complement(23996..25171)
                     /gene="gguB"
                     /locus_tag="PKU88_00825"
     CDS             complement(23996..25171)
                     /gene="gguB"
                     /locus_tag="PKU88_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPX67865.1"
                     /translation="MGIVNEARDLIKKNIREYGMYIALVVIMLTFSMATDGLFMSSRN
                     ISNLINQTGYIAVLAVGMTLIIVIRHIDLSVGYIAGFLGAIAAILLTKYAMPVWITIA
                     VVLVLGASIGIIIGFLVTKMSIPSFVVTLAGMQVFRGALLRVTSSTGTIIVPNADFNA
                     IGNGYIPKLFSINGVHGLTLVLGAAAIVLYILAEFNNRNNKLKYGFEVLSNVMFTVKL
                     LFVSLVIGYITWILAGYNGFSWTVVVVIIVVLVYNFITEKTVLGRHIYAIGGNPDAAK
                     LSGINVNRVSMIVFSSMSMLAALSGILYTARLQSATTTAGLAFELDAIAAAYVGGASA
                     AGGAGKVTGSIIGAFVMASLTNGMNLMGVDISYQYIIRGAVLVAAVIFDVKTRNKKA"
     gene            complement(25183..26706)
                     /gene="gguA"
                     /locus_tag="PKU88_00830"
     CDS             complement(25183..26706)
                     /gene="gguA"
                     /locus_tag="PKU88_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPX67866.1"
                     /translation="MKEYILKMNNITKVFPGVKALDNVNFQVENGEIHCLVGENGAGK
                     STLMKVLSGVYPFGEYSGDIILDGSVRKFAGISDSEKAGIAIIYQELALVPEMTVYEN
                     IYLGHEIKKGRLIDWNETILQAGEALKKVRLNNLNPATKVKDLGVGKRQLVEIAKALS
                     KNVKLLILDEPTAALNEDDSENLLELLRELKEHGVTSIMISHKIKEVIEIADNVTVLR
                     DGRTVASMSARDGGILEGNIIKHMVGREIENIYPKRNVQKYEEKTLEVNNWNACDSKT
                     GREILKDMSFFVKKGEIVGIAGLMGAGRTELAHSIFGNPDEYKISGDLYVMGQKKHFR
                     GPHEAIKAGVAYVTEDRKGNGLILISDIKTNITIANLKALAKQGIVNENEEVKTANYY
                     KGSLDIKAPSIEQKLENLSGGNQQKVSLGKWLYVKPKVLILDEPTRGIDVGAKYEIYT
                     LMNRLVEDGMSIIMISSELPEIMGMSDRIYVVSDGRITGELPAEGATQEKIMEMATN"
     gene            complement(26823..27941)
                     /gene="chvE"
                     /locus_tag="PKU88_00835"
     CDS             complement(26823..27941)
                     /gene="chvE"
                     /locus_tag="PKU88_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPX67867.1"
                     /translation="MKRLIAVLMAFAMLFALTACGSAPASGETGGDSKTVDVGIVLPT
                     KDEPRWIQDETRFSEALGSTDYSVEILFSQGSSAKEKENVDALIAKGINVLIICPHDG
                     DAAASAVEAARKEGIKVISYDRLITGTDAVDYYVTFDSIAVGEAQAKYLVDQASGSGN
                     PLYLYAGAASDNNAFLFFEGAWNVLQPKIADGTFVIKNSSEAVALQGKAKLTRDEMGK
                     IIGQVTTNWDFNEAKNKAEAHLTAAQAADKGNVFILAPNDGTARAIADAFAADPAVTS
                     YKVTGQDAEKASVQYIIDGKQSMTVFKDVRTLVKDSISMAVSLLEGKEPQTTGSYNNG
                     KIDVKAKQTEVIVVKADNVKSALIDSGYYPASDFTNLK"
     gene            complement(28158..29228)
                     /locus_tag="PKU88_00840"
     CDS             complement(28158..29228)
                     /locus_tag="PKU88_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPX67868.1"
                     /translation="MAKENKVKNSIIVVLFTVFLMLILSIASILNRTDRGFFQSNKNI
                     EEREKIKIGFSLGTLKEERWIKDRDILIAKAREFGADIIVQNANNDDQDQMKQVRYLL
                     EQNIDVLIIVPNDLNKAAHAVELAKRQGIPVISYDRLVLNSNVDLYVTFDNVKVGELM
                     AERITDSMKGENLLIVNGAKTDNNTKMIKQGYDKILGPLEAEGKIKIIAEEWAADWLK
                     EYAFKVVDDMLQKGIRIDAVISGNDSLAEGVIEALSEHRLAGKVSVTGQDADLTACQR
                     IVEGMQLMTVYKPIEKLAEAAVEMAVRLVKKEPLNVQDYINDGTYEVKYYVLTPIAVD
                     KENIDETIIRDGFHLEDEVYRH"
     gene            complement(29221..30831)
                     /locus_tag="PKU88_00845"
     CDS             complement(29221..30831)
                     /locus_tag="PKU88_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX67869.1"
                     /translation="MTKLLIADDEYLVLDSLKMIIRKNMKDIDIVATASTGREAIEKA
                     IELKPDIIFMDIHMPGIDGIEAIRQIKAVNSSILFVIITAYEFFDYAKEAINLGVSEY
                     ILKPIEKSKVIDTLNNLCNTIKQNRRLLLKEAELKERINRIIPFIESQFVSYQLFTSG
                     TISDIEFYEEIFNINLRQGYALTVLLKNFEGKIKEDNLKLSLEKQNFYEKFSLELKRI
                     CPCLIGNPLLDRITAFIPSEKGIDSYEIRNESIKIANRIIDRIKPGTGIEYTIGIGRS
                     YEAGSFNKSCNEAYIAASVSNGHAVMHYEDITPSNNIPDTYPAQKEAIFANRMLTGNL
                     QGAGEAFEEIYLWLITNYYEDMDKIKSKLIDLLFVIEKALPYKASSFSKSKQSYIFSI
                     LKTNSKEVLKRQFMDIISQLSEEIQEQIRSERDGIIPKVLEYLNNNYHKEINLADAAR
                     YVNLSYHYFSKIFKDEAGKNFSDYLTELRIEKSMGFLKDHKISIKEVCFKIGYNDPNY
                     YCKIFKKITGMTPTEYRAVINIRGDDIG"
     gene            complement(30809..32350)
                     /locus_tag="PKU88_00850"
     CDS             complement(30809..32350)
                     /locus_tag="PKU88_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPX67870.1"
                     /translation="MNPGLLTFIGIRKKLIMYFLLTILLLGVISIFSYYNARVVLRNT
                     NSIITDYSYLNSLNNDVNSLMTELEKYLMTASSESLLNYYTHYNILQETTRKIPRTAE
                     YNNELLLMKDIGNMIDELLIETDNAIQAKRGRISSRYISHFQRSNRISDYIKLYNNKI
                     LDSKLKSGTEKYENINKTMNFISYMNLLIIIAALLANIYIAVVSTYRLTKPISELSYS
                     AEKIAEGDFDIELRQTHTGDETDILAGAFIKMVNSIKAYINELKRQAEVEKRLKEQEM
                     QNLKMMSHLKEAELKFLQSQINPHFLFNTLNAAAQLAAIEGADKSSEFIVSISNLFRY
                     NLKNIDELTTLEDEIQYVKYYMQILKTRFGSRIDFYTDIDETTFDIMIPRIVIQPIVE
                     NAFIHGLQNLERHGEIHLNVRREGDCIMVEVIDNGIGMDSACIEEILRFEEDGSKYRN
                     RLTGIGMSNVLYRLQLFFNIEDPRKLLSIESEIGHGTKVTLKLPIMKGARREGEDTYD
                     KASDS"
     gene            complement(32386..33387)
                     /locus_tag="PKU88_00855"
     CDS             complement(32386..33387)
                     /locus_tag="PKU88_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPX67871.1"
                     /translation="MKILRNLLRFLYVHAILILTLMVIAASAVLFWLQREDIKTYISK
                     PEYHFYFIAQNSVDPFWNEVMQGVQNSAEDNNVVVEYNAPRFSNPEEELQFMDIAIIS
                     KVDGIITHVGGTGFTEMIDKAYDNGIPVITVENDDSESKRYAFAGTNSFELGREAARL
                     LIEATNADAKVAVILNGDLEQEAIEYNLKMNGFISTLKEYPNMKIIRTYTSKMGILSA
                     EEITQSIIDSDEGINAIFTAGSADTLGSAQLIVDRSKVGKIVLVGYGNSEEILRYIDK
                     EIIYGTVASDPYKMGYESVRALVDVKNGKSVPSFIDAQVKVIAKKNLSSYKKAYKLD"
     gene            complement(33543..34082)
                     /locus_tag="PKU88_00860"
     CDS             complement(33543..34082)
                     /locus_tag="PKU88_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67872.1"
                     /translation="MKRVISFALVLLIVVPLLGCVTDNSNDKASDKAAQNEIHIRVGQ
                     QDINYTLTSRPFTSKEDFKKAYLEIDREKLIYVPRGTKITLDMKEQDVKASVKAYWIN
                     REGAPNYNDSFGAPLDMDVEVEKIGDSKYEFTAERLLQAGLSSHYEENEKITAGYVAE
                     LQKNGETRYCFFIIQIDKN"
     gene            complement(34079..34654)
                     /locus_tag="PKU88_00865"
     CDS             complement(34079..34654)
                     /locus_tag="PKU88_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPX67873.1"
                     /translation="MLDAGCGEGYYICRLKNSLTDFCDCIKTDIYGIDVPKAAIHYAS
                     GRDKGIRFAVASNYHIPVLKGTLDCILCSFAPRDEGEFRRILKPKGKLIVASPGARHL
                     YSIREALYESPEEIGQKGTVGEGFTLLEKKKVNYEIILKSKQDILNLFTMTPYSRHAE
                     TSALEKLSEFATEVDINIFVYSARPAGRSFK"
     gene            complement(34676..34954)
                     /locus_tag="PKU88_00870"
     CDS             complement(34676..34954)
                     /locus_tag="PKU88_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67874.1"
                     /translation="MARIMMHSIFQCPNCFKALKQGERQYFCPTGHSFEIAKKGYVNL
                     LLPSHTGAGTPGDSKEMLQSRREFLDKGYYEGFSNMLNVIVLSALLID"
     gene            complement(34942..35262)
                     /locus_tag="PKU88_00875"
     CDS             complement(34942..35262)
                     /locus_tag="PKU88_00875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPX67875.1"
                     /translation="MSDKREGFFKRVYEIVGRIPEGKVAAYGGIARMLGCSGGARTVG
                     WAMRSAPEDMKLPCHRVVKATGELSPGHVFGDPEIQRAMLEAEGITFRADGTIDMERH
                     LWQG"
     gene            complement(35277..35666)
                     /locus_tag="PKU88_00880"
     CDS             complement(35277..35666)
                     /locus_tag="PKU88_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016767347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPX67876.1"
                     /translation="MKIEHIAIWTKDIERLKMFYTNYFDGIAGEKYINPKKSFESYFI
                     KFDSGARLELMQMSGSPLDSNDTINQHIGLTHIAVSTGSAEKVNSLTDELRKAGYTVV
                     SEPRHTGDGYYESCILDPDGNRLEITV"
     gene            complement(35879..36268)
                     /locus_tag="PKU88_00885"
     CDS             complement(35879..36268)
                     /locus_tag="PKU88_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPX67877.1"
                     /translation="MTEKALTMKLMEGLYGVCRLDKDEAIPEWVSQCSFYSVTKTLDE
                     LSVVCSQDNIPDKIKCEKDWRILRVEGPLDFSLIGILSSISTTLANKGISIFAISTYD
                     TDYILVKDKNIDTAINALSAEKYEIIR"
     gene            complement(36336..36842)
                     /locus_tag="PKU88_00890"
     CDS             complement(36336..36842)
                     /locus_tag="PKU88_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67878.1"
                     /translation="MKHKAYKAAVWAGAFLFLLSSIVYANSSWHWVTASPMKVLPFAV
                     VFTILIETAFVTKLGGVSDSRKAFYIICLANLMSFIAPYLERAYRFIPTSGGYSIFAA
                     FNKGPYYIVLAGYLILTVAVELPIVYFLLRKDSLNRKNLIVSILLSNIVTTLLVAVCE
                     RIICVGQW"
     gene            complement(36902..37063)
                     /locus_tag="PKU88_00895"
     CDS             complement(36902..37063)
                     /locus_tag="PKU88_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67879.1"
                     /translation="MAVSLGDIEKVNSLTYELMSADYTVVSEPRYTVDGCYGNCILDP
                     DGNRLELTV"
     gene            complement(37077..37793)
                     /locus_tag="PKU88_00900"
     CDS             complement(37077..37793)
                     /locus_tag="PKU88_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67880.1"
                     /translation="MCKKDKLLLIMILSMAMLIIVSAVCYGNSAEPPSILIIAPDAPD
                     ELEISIGPADIKASMRDKIIERYYVFYSADLRIAKDYNINISTGEDSFEVLLEEPLKT
                     YNNIYTLDLENQTLTPGKSLSRSILLVSLRVILTLIIEAIVFWLFVFRKKNSWLAFLI
                     INLITQGALNIWLNSSVPLASYLVFSLIFAEFFVFIAEIVLFLTLIKEHSRRRTVLYV
                     IAANFLSLFAGGYIITVLPV"
     gene            complement(37856..38095)
                     /locus_tag="PKU88_00905"
     CDS             complement(37856..38095)
                     /locus_tag="PKU88_00905"
                     /inference="COORDINATES: protein motif:HMM:NF022308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2651 family protein"
                     /protein_id="HPX67881.1"
                     /translation="MEFLMALLIFPLLSFLFGIIGQVLIRKIFIVAGITFALWSIAAF
                     TVFNSSFLVWVFVYTVLSALGASIVHFGKDLKKRS"
     gene            complement(38279..39439)
                     /locus_tag="PKU88_00910"
     CDS             complement(38279..39439)
                     /locus_tag="PKU88_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67882.1"
                     /translation="MMIVKKPNVLLSTILAIFLIMAFVSGCNDTGKSHKEITALTDEE
                     LSYFNGDEFFNGEYLNIRNQFLSSTYERPEKIDIFQLFYCGSGIEEISTEEERAAVVA
                     YNNWDMEPDCACMKISRSNMESVLGKYMGLTLADTDEIGLENFTYLEEYDAYYYYHGD
                     TNYRMEIIFSGGERDGDIVRLFYDDVFFSDGKKILTLQEKDGSYLFVSNVYCEKPIED
                     GNKADVGLDQRSDGSIFSLSIPGTGTLSLNAYPVDFPFSLDLTEKSRKSFRAESFGNV
                     TVVESDNLQVTYLNPDEGVHTIITIRTVKEGCSSAGVTIGDTEETLLEHWTEKPLKKM
                     DSISYDDEVWFGKCDLAYAHTPEEGTKSVVFLIEDGLVSGIEIINGLDGAIY"
     gene            39833..41116
                     /locus_tag="PKU88_00915"
     CDS             39833..41116
                     /locus_tag="PKU88_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPX67883.1"
                     /translation="MNLNEKIKILSDSAKYDVSCSSSGSSRKNKKGGIGNAAPSGICH
                     SWTPDGRCISLLKILLTNYCIYDCKYCVNRSSNDVPRAAFTIDELVNLTINFYRRNYI
                     EGLFLSSAVCVSPDHTMELLLKSVKRLREEVNFNGYIHVKAIPGASNKLIEETGKYVD
                     RMSVNIELPSGESLKLLAPQKTKESIIKPMGLIKSGIIQYQEEKLKFKSTPQFVPGGQ
                     STQLIVGATKDNDLKILKLSESLYSSYKLKRVYYSAYVPVSNHPNLPAISGPPLLREH
                     RLYQADWLLRFYGFNSGELLNEENPNFDELLDPKSGWAIRNLDKFPVEVNKADYYTLL
                     RVPGIGVKSAQRIVQGRRVSMLDFDDLKKLGIVLKRAKYFITCKGRYFPQKSIPTNAV
                     SIKNRLLLEDNIKNRGNIEQLSIFSLFPGIMDGEF"
     gene            41116..41853
                     /locus_tag="PKU88_00920"
     CDS             41116..41853
                     /locus_tag="PKU88_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPX67884.1"
                     /translation="MLYYIYDGSFDGLLTAIYDAYYNKDFPEQIVPQDSFTDNFLINK
                     VYIRTEEEKSRKVHDAVLQKISRRAMAGIMYAYLSEDENAGISILEYLRFGFKVGRTV
                     DSYLADDRVNNINRLVTKVAREKHLLAGIIRFRLLHNNIYYAPIEPQYNTLCLLGKHF
                     ADRMSDQIFVIHDVKRSYGVFYDKTKWFMSDIEMNKDIVLHENELFYQELWKRYFDNI
                     TIRSRINPKLQSNHMPKKYWKYLIEMK"
     gene            complement(41854..42576)
                     /locus_tag="PKU88_00925"
     CDS             complement(41854..42576)
                     /locus_tag="PKU88_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HPX67885.1"
                     /translation="MSRFIIEQPFWDIFPECEIGVLLINDIDNTEEGCEKLRSDIIEF
                     LEQSNQEARKYLTEPVLSQNRVISVWRDAFSKFKTKKGARSSIEALLKRIEKGNEVGV
                     INPLVDIYNGISLRFALPCGMEDVDTFKGDLRLTVTNGGDPFLALGDEEIDYTLPGEI
                     CYLDDEGAVCRCWNWRDGQRTMLTEDTKNAIAVIESVDPLRHKDLEAALDTLSAWIEK
                     VRIGKVNTKVIMGKNNSTLTTR"
     gene            complement(42882..45665)
                     /locus_tag="PKU88_00930"
     CDS             complement(42882..45665)
                     /locus_tag="PKU88_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPX67886.1"
                     /translation="MVIDFERPLMLFLIPLAIFFVFFTARKLRKMYRLRRRLILFLRN
                     SVFVLLILALTGVNIKWIVDTTTTIFVIDASDSMREYRTTSEDFVRKALEKKGSKDQT
                     GVVAFGDNSLIESFVSGDSVFNKIETEPRGIYTDIEGALTTSVSLLPQDSKKRIVLIT
                     DGEENEGNSSKLASSLIGQDIDFKVYRINREIGKEAAVESVTVPKSLRVGEEFNIIVT
                     VESTVQTEAKLTLISGKDKAAEEKVALQKGSNRFVFRDTAETGGFKSYKALLEPDTDT
                     ESRNNEASTFTNVLDRPRILIVEDVAGEADEVARMLEATGMNYVRVNAAGVPGTLEGL
                     SAYKSIITCNVSAENLSDGFLNSLDSYVKDMGGGFIAAGGENSFALGGYYKTPLEKVL
                     PVHMELKGRKEIPDMSIVLVIDKSGSMTEGRGGIMKLDLAKEAAARTLESLRPKDQIG
                     VLTFDDTNYWVVPTRKADDAEKIRDDIGTIRPGGGTSIIPALQEGYDSIKKTDTKIKH
                     IILLTDGQAERTGYTELVEKLAGDNITVSTVAVGQGSDVQLLQDIAKGANGRFYYTDE
                     FANIPRIFAKETFMAARAYLNNREFVPAITNTHPVLEGAAEEGLPALLGYVASSPKSA
                     SRVVLASDQEDPILTVWQYGLGKTAAWNSDINGRWSRNYVGWENNIKLWQNLINWTIE
                     KYREDDITVEAEVQGGKGTITLNNKADKGEFESKASIVSPSLESSEITLYPVAPGQYR
                     GDFEITETGSYIIKALQQKDGETINAASTGISVQYSPEYRIQPERGYLDRLIKEAGGT
                     LINTPEDVYKGEIKDIFGTVDLTPALLILALILYMLDIALRRLNLPLAAVEEKLSAAR
                     ARLIPQIKRSAKVKRPISTMDEALKAAEKKVHMPPQAEGKPEAKEKEAKPEKPKTENL
                     DTSTLLKKKKSRE"
     assembly_gap    45705..45804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45805..46682)
                     /locus_tag="PKU88_00935"
     CDS             complement(<45805..46682)
                     /locus_tag="PKU88_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HPX67887.1"
                     /translation="MTLFSPWALWFLSFMPLVVLMYILKQKFEERQISSIYLWQQVLK
                     DIEVNTPWQKLKKNLLLFLQLLSILFLVFALSDPYLYTGGGMHSNLVIVIDNTGSMNA
                     RYENGTRLAKAKQLAEEMISRSGTKANITLLTVGRSPRVEIGKTTDKGEAISKLRGIN
                     PGNSSGNINDSVSLVRAMIKQYEGSDSYKAVFYTDSPVNTEDLNAEVVPLASELVNVS
                     LDYISYSDDNGKLTVLVRASNRSNTSLKREISLYGNDKVMDIKNVEIPAGETMSVYFE
                     GISANAPYIWAELTEK"
     gene            complement(46687..47565)
                     /locus_tag="PKU88_00940"
     CDS             complement(46687..47565)
                     /locus_tag="PKU88_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPX67888.1"
                     /translation="MGERIFDSDFLKKLDTIVINVRIMLNTGSGGNRKSRSKGSSVEF
                     SDFREYSLGDDFRRIDWNAYGRFDRLFVKLFMEEREAMVNIFVDSSRSMNFGEPKKSL
                     SALKLSGALAYLALNNLDRVCLNSLAGTLVKQSSVLSGRGMFRQCADFLENMQFDGIT
                     DLNSCIKKKEFKGSGVSIVFSDFFTTGGIEDAIKYLLYKKQDVILVHVLAPEELSPEI
                     EGQIRLLDSETGETRDISVTPALLNQYDKKLNSFINSIKEFCSRMGATYIQVSSEEPV
                     EKIVLEEFTRAGIIGK"
     gene            complement(47578..48591)
                     /locus_tag="PKU88_00945"
     CDS             complement(47578..48591)
                     /locus_tag="PKU88_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPX67889.1"
                     /translation="MEINEKQIRDIIDVINNVEAEIGKAIIGQKDIVKQVLISILAGG
                     NVLLEGVPGLGKTQLVKTLSKVMELSFSRIQFTPDLMPADVMGTNIIVKDDSGNSRFE
                     FQKGPVFANLVLADEINRATPKTQSALLEAMQEQTVTVGKVTYSLPQPFLVLATQNPI
                     EMEGTYPLPEAQMDRFLFKLNVLFPSFDELKGIMDITTSNKEVELNKIVDGESILEMR
                     KIIREVPIAKPVQEYALRVVLGTHPEIEGAPEGAKKYIRFGASPRAAQAIITASRARA
                     LMEGRYNVSFEDIKYVAHPSLRHRFFLNFDAVADGITTDGLIDQLLDEISADIVPARH
                     IKG"
     gene            complement(48633..>49055)
                     /locus_tag="PKU88_00950"
     CDS             complement(48633..>49055)
                     /locus_tag="PKU88_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67890.1"
                     /translation="NGRQGQGNQPVGNGQGSGQGSGAGSGTDMGNEDQAPIPSGSSGI
                     SKKDGSVKKDGEYEKIFTPQTLGGDGTTSNLSGKKGTGGTVEQVITDKSRTVRGTSVP
                     YNQIIGRYKDRAMESISASDIPPGMKDMVKEYFTSLKE"
     assembly_gap    49056..49155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<49156..50129)
                     /locus_tag="PKU88_00955"
     CDS             complement(<49156..50129)
                     /locus_tag="PKU88_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67891.1"
                     /translation="MSPVDKELIRMVRGIRDRLHAAFIINSIILGFILALCLGIGVAV
                     AARFVPIYNVFLTIFKIAASVVLAAFLYSSFRTPGNDYAALKADSMGLQERTVTALEL
                     IGSQSAFAVLEKNDALAHLKVTDYKKKIPLKPNRRYLLICVILTIVLALSGFIPNPMA
                     ERAEELHSLKEKITEQQKKADKLVEKVKRNPKLSEEQKKELEARLAELKQELKAAKDE
                     KEISKALGRAEKKLEYIKEKYAKVEDLENIVDVLSKNEMTKALADMIKNGDEKAFKEN
                     IRKMAEALRKLSTVEKQKLAEELSRLAQEIKNNPELSKAFAGLAEKLAS"
     gene            complement(50159..51070)
                     /locus_tag="PKU88_00960"
     CDS             complement(50159..51070)
                     /locus_tag="PKU88_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67892.1"
                     /translation="MNINPVLLKELKVRMRGWKAAGIIALYLLVLTVVAVFIIYTTFM
                     DPYSSNIDPQVSIGAYTALAVFQFMLIMFIVPALTAGAISGEREKQTLDLVLCTRLKP
                     ISIITGKLFASTSQTLLLIIASFPLFSMVFLFGGISIKEVMQLFGFYIVTAVTIGCIG
                     IFFSAHLKRTTASTVFTYGTLAFLAFGTIFIGVFYIRIFYNWDYNKFLPILYSNPLTG
                     FGSLLAEQFGYYGGINAFLGLSAGSGQNPNAANAISPWLGNIIFDIVLSAVLLILSAA
                     RINPVRKSIFSYIRFRGRKKKKASDSI"
     gene            complement(51067..51996)
                     /locus_tag="PKU88_00965"
     CDS             complement(51067..51996)
                     /locus_tag="PKU88_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX67893.1"
                     /translation="MLSIRNLNKRYGRFQAVSDLNLDVPEGEIFGFVGPNGAGKTTTM
                     KIICGLLRATSGEITLDGVDIIQNSRRMKEKIGYMPDFFGVYDDLKVSEYLEFYASIY
                     NIKGQERKRITDDLLELVDLGSKREAYVDSLSRGMKQRLCLARSLVHNPRLLVLDEPA
                     SGMDPRARFEMKEILKNLKGMGKTIIISSHILPELAELCTSIGIIDQGRMVISGSNEE
                     IMQQVYNKKVVRLKVRDRLDDAVLILKEYPFVDKLVIGENTIQAGFGGGDGEMSRVLS
                     NLINRGIPVVSFAQMDGNLEDVFMKVTKGDDEQ"
     gene            complement(51996..54401)
                     /locus_tag="PKU88_00970"
     CDS             complement(51996..54401)
                     /locus_tag="PKU88_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67894.1"
                     /translation="MKKVLRAAAFILMLALIIGRPAMQLLQAEAKDEIRIDMKVGFDK
                     FYKIGFAAPVYFEIENSLRDINGELQVEMPNQYGSITIYAINVNLPRNSVKKFLMNVP
                     MNVFNTKINVNLAEGDNIISKKSFRIDPGLSADTYAIGMLSDDFDSVKYINKVSMKSF
                     GNISTKNVKLDEDSFPEDMDVLKAFNVIVINNYDTSRLSTAQYETLKRWVSEGGMLII
                     GTGPSHNKTLAVFKDDFITGEVGEVSTLTTSSLNEMAGSKTGPAMSISVSDITMNNST
                     PGIKDGDFVLLQKIEKGKGVVGVASFDFGMEPLSTWVGNSAFADNLIVGIMPQYYFSD
                     IYQKRIIVRDDFYAIDNALRNIPELPQPKPFHMVFIYIAYILLAAPISYFILKRMDRR
                     ELMWLTVPVLSIAFSGVVYLTGAGTRLTGPVTNVISLVDIDNSGIISPKVYAGVFTPN
                     KDNIRIEAAGDFKVKPLRMNDNYYRGPIDNENTSGRIDSKITLAPRTVLEFYKSGVWS
                     MKTLAMETGKEFSGRLESNLNYAKGSFTGTIINSSGFDLDECYIITPTQYAGVGPVKN
                     GETKQIDVKPSSYFGQRYELINAIYKDPYSGPNRVNQNSRLTPEEMAEFRLNLQKRHV
                     LEHSLMYEAYQGYEAQLIAWSSTPVAEDLLVNGRETRKYEKAFITSKVNLRFRDGKNV
                     EYPLGFLKPEIVNNLDAGNYDDYGKTFYGRGSFEVHYKIDDIRAESINIQYTVGDTQR
                     VKQYIWDNEEKEWDEGDYRDFEIHGELLGKYLDGSSMLKLKIELSDSDAQLPRISVKG
                     SVK"
     gene            complement(54652..55224)
                     /locus_tag="PKU88_00975"
     CDS             complement(54652..55224)
                     /locus_tag="PKU88_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67895.1"
                     /translation="MFKKKKNYLKQIYKMNPDTNAYIIEVSLIDYNEIFNGWDPSPIK
                     RRDVDPELISFLEECDSDIPLEFPLELMFYLPEDQYDKEKEKLSRVGIKNYFDYSVHF
                     IRKELLQIRQKIFFYIVMAVAFLSVGYLSGQKAEPGFFTVILIEGLTVGGWVFLWEAF
                     SLFFFARQEVSGRLKRYIRLKNTKISFKYN"
     gene            55407..57059
                     /locus_tag="PKU88_00980"
     CDS             55407..57059
                     /locus_tag="PKU88_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPX67896.1"
                     /translation="MNIDRMKNLLFDLCSIPSISETPGEIEMAEKIYETVMNMDYFKN
                     NPANAGINPIKNDPFGRYYVHALMEGKPESKKTVILLSHFDVVNVEDYGAYKDYAFKP
                     LEYTELLKNGSGISLSKEAETDLASGDYIFGRGIMDMKFGLALDVEIMHQIGNKLDNF
                     PGNILLLSVPDEENNSAGMLASVEMLAALKNEKGLEYVCCIVSEPHFPKYPGDNSKYI
                     YTGAVGKLLPVFYCVGKETHAGDPFSGLNPNLLTAKIIEEIELNPELSDVSASFRTPA
                     PVCLKQSDTKNSYSVSTPAAAYTYFNFITVTSSPDEVLNKLMGISKKTFEEVLHNIEN
                     KADRLQALTGSKPKLPSIKPMVITFRELYKMCLEVGEKEFDEHMESFVKKSPESDLRE
                     LSVDIVREAHKFCPYRDPMIVLFLAPPYYPHSGARGQNGKIAEITTKIIKKANSKYGV
                     NLYLEPFFPGLSDMSYLGLPDNIDVESLKTNLPLWGDKYTIPLDAIASLNIPFMNIGP
                     LGKDAHKYTERINIPYSFEIASKLVFEAVLSALDASPEDFGI"
     gene            complement(57066..57608)
                     /locus_tag="PKU88_00985"
     CDS             complement(57066..57608)
                     /locus_tag="PKU88_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67897.1"
                     /translation="MKRYLNYFAAMFLVLAVTDLCLGGALTGFRGQNSDSAPEGLNEV
                     NNIQIQPEVQENAEVMNIDELGEFIASKSCEFVQAAFERDRSKVESMLCKGTMYVVSE
                     DNSSYIRYTSGDLHVEGYMATDRKLMQVRQSWHITEDDGTIASGVEVFIEGEEAPQIW
                     YIHYRRSFGRWKIFMLENGI"
     gene            complement(57690..58757)
                     /locus_tag="PKU88_00990"
     CDS             complement(57690..58757)
                     /locus_tag="PKU88_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX67898.1"
                     /translation="MNLPSSILKPGMTAYEDIFNDLGVLILNKNTILDQWQIEKIHAN
                     GVDRVRVRLTENDSLYAVQEEEIPSIYDPGKIRAFKEKYVAKVDEVTYIIKEIGRGAA
                     VDVQTVGQISRHIIREFSTVSEVINYLHLARPLDDYTYSHSLNVSLMSIIIAKWLNLG
                     NEEIDEIATAGLLHDIGKTKIAERLLQKPGKLTKSEFEEVKKHTIFGYMMMDNILDAT
                     ANMKYSVLMHHEKIDGSGYPIGSLDEDIPLFPKIVAIADIYDAMTSNRSYRNRMCPFE
                     VIKNFEMHTFGKLDTKVLTVFLKNIANSYINDFVELNSGELCEVVFINPNRIWQPIVR
                     SGADYIDLSRDKDKRFIKAII"
     gene            complement(58910..59908)
                     /locus_tag="PKU88_00995"
     CDS             complement(58910..59908)
                     /locus_tag="PKU88_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67899.1"
                     /translation="METRIWCEFTPPEDVCRDYIIDAFKRYNVILNYKLEYGHDNEGF
                     YDMVRTYNDHHVPLSIWATLSDEMGYWINEKNAEQFDRYVRELAERFEYKGLKIKGLC
                     IDLESPLQDIKSLCEPKNIIGLLITCGKMLTANLNRKRFTEAGRILSETARFLRSKGL
                     ESYAACIRHCYYDIRFKSELIQNALEIPVFGVGWDKYNLMYYSTMIRNELRKIRKTDV
                     DYLMYHQISYLKEVLKDKLAVSIGVTNAGKMGNEPYYENISEFEKDIGILKEIGINSF
                     SLFSLDGIIEKSQLLGYLAAMERAKPYKPAICSRVIRNEEISGLLFSIGKLYYRLR"
     gene            60336..60998
                     /locus_tag="PKU88_01000"
     CDS             60336..60998
                     /locus_tag="PKU88_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPX67900.1"
                     /translation="MAASYRMLKGYADTCFVEKKSRFISYAQPVYSEEEALQFLAGIR
                     KKHYDATHNCYAYILGESMNIQRSSDDGEPSGTAGVPILEVLKKEELTNSIVIVTRYF
                     GGIMLGAGGLIRAYTQGAVNGIKAAGTVRMQPFAVYELKMDYSLLSKLQYEISKKDYI
                     INSIEYLENVTMRVLAASDLKDAFEKDIAQWTNGGILTESLGEQMLKVDEESGMIVTN
                     QS"
     gene            complement(60989..61501)
                     /locus_tag="PKU88_01005"
     CDS             complement(60989..61501)
                     /locus_tag="PKU88_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67901.1"
                     /translation="MGKKFKCPMMCDMHMMHGVAPMDMMPYMPLAEEIEMDNFYEDEE
                     DDRHFMKMYPESCRKIMIYVKVEVDRMEGKDKEIHDYRPDREMINMMTDNAYNRMVKD
                     MPEMAEGEETRQYPARRFSRDLLRLLLLNELFRRRRRRRRRNSYGYPFGGYDFDFDND
                     DFDFDYDNYD"
     gene            complement(61640..62443)
                     /locus_tag="PKU88_01010"
     CDS             complement(61640..62443)
                     /locus_tag="PKU88_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX67902.1"
                     /translation="MRKLSVFSLFDNEKRFNLDAFEMKVKELLTRQNSLVILLNSPAQ
                     NPTGYALSDEEWEQVIMIIKREALDESKKITLICDIAYIDYAGRASDTRRFMRLFSGL
                     PSNVLVVVAFSISKSHTMYGLRTGAAVCVSSDKGVAEEFSDVFEASNRGTWSNGTRCG
                     MKLLSVIMNDKELLRKVDEEREQVRLLLEKRAEVFTNEAKSVKLDICPYNAGFFVTIP
                     AAQPEESCKLLKKDNIFAVPISRGIRFAICSVPLYKMKGIAEKIRKAVK"
     gene            complement(62474..62881)
                     /locus_tag="PKU88_01015"
     CDS             complement(62474..62881)
                     /locus_tag="PKU88_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67903.1"
                     /translation="MNESFSMVAKHVNTVRSEDKIFALNREAGNAVLQFGKEKVVNAT
                     VGSILDEDEKLAVLPSVLETLRNLPDSEFAAYAPILGTPDYLESAIQAAFKEYRPEGY
                     IKAAATPGGSGALRQTIWNYSEIGDTILTSDWY"
     gene            complement(62940..64982)
                     /locus_tag="PKU88_01020"
     CDS             complement(62940..64982)
                     /locus_tag="PKU88_01020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPX67904.1"
                     /translation="MEDNKSYITKSLLLWGALLVISILLGYSIYNAFSGRIYANVFKS
                     MNNNLRFAESSYGVFMNQMKMGMLQASVETSIKEMITEKDSAALEDLLKKWGEHRDYV
                     DEWYVVGEDGNIISSAGKGKDLLIKASGKTKIQEIVGEALSSGTVITGTELIPIGSRG
                     EFTLVQYVIVPVMENDRGVIGGIVTLMNLDGDDNITGKIMSDSGMHSIISAKDRIIAS
                     SLESELYRLNKGNSLPEDIRKEVLEKDTPFVKRLEIFRGSWEDEDSYFGAFRAIKDIE
                     GNIIGVQGIIHNDPLAETTLGQIRDFTVIAVILLAAVFSIIIWSYINAQKVLFKEKQF
                     SNRLSSIKRFSDLVRQASNEDEVYEILFDVLKKKSGITQVVVAKKEYNDKCLTLYKAM
                     DMEKIQSIKNTMTVEENCWAVKSGKEFINNGNNNDFDCKDFYSESASYICLPIILGGI
                     VSGVIQIQSDMKNYFNDDLIAEIKVYIDNITPVISNIRLLESLNNMASIDTLTKAYNR
                     RYLEKYLEEQIKISKENNLHLSIIMIDIDFFKIFNDTYGHDAGDYVLMYFADTLKFNV
                     READVVSRYGGEEFIVVLPRTNHRGAYRVAEKLRKKVEEMSLTAISNENPPKITCSLG
                     ISVYPLHGNNIDMLIHSADKALYKAKNSGRNRTCISGDDNEREGIQERHDDINNTI"
     gene            complement(65100..65912)
                     /locus_tag="PKU88_01025"
     CDS             complement(65100..65912)
                     /locus_tag="PKU88_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HPX67905.1"
                     /translation="MEKEIKKLMQISAIITSILLITALISFLYVLKIYPASNRRIIVY
                     MIAGVSVSLLLIWGVSLELSRAYRTGKAKGFSIYVLEAGIGILLPLYIYLTGLFSGDK
                     DDIRRLYININNIAVRHNLQKNRPSKILMLLPGCMQDKDCSCKITDDMRNCRRCGGCR
                     IGEAADLVERCAVRAIVVKGGTAARNTAKEFNPDFILAVACERELLSGMADVGRIPVM
                     GVINQRPNGYCTNTTVDILQLKEILAALEEKGPELRCTKQGVKRDVKVQKNY"
     gene            66168..66623
                     /locus_tag="PKU88_01030"
     CDS             66168..66623
                     /locus_tag="PKU88_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67906.1"
                     /translation="MALACFYGAIAAAWIIFLICALKKSISFKHIMVAIAGMGFSLLY
                     ETALGEYAGLYHYINQSDSLLYIIISGVFLYPVIEMLYALFLPERIKPAIVYTVICIM
                     LLLVFEILSLYTRTIVFTGWRIVPWSIATYILTFVWVNLLFRYLKKKGL"
     gene            complement(66712..68196)
                     /locus_tag="PKU88_01035"
     CDS             complement(66712..68196)
                     /locus_tag="PKU88_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPX67907.1"
                     /translation="MKEKKFFAVIIALLFVLMLAVGCSYNQAAPAITTNPIPADAEQP
                     QETTVTEVYEAPDPIESDQDEKLGVVYISASKLKVRESAGTDAKALDDLIKGTAVDIV
                     EEKMDDSGKAWYRISYDGITGSNEGWIAAEYTVRDRTELLDENLKKLDFSPQNKTYEY
                     PGNPRVQARGIYLTKYSASNDRLDKLIEMTKRTKINTFVIDVKDDKGHMLFPTKAAEK
                     YSPEANKYATVKDIGALMKKLKENNIYTVARIVSFKDPTYTKQHPDRAIIYRDSGKPF
                     TNSDGLIWASAYDKVLWEYNLEVSKEAAETGFNEIQFDYVRFPASDGGKLDKSLDYRN
                     NEGESKPQAIQDYLKYAREQLAPLNVYISADVYGLVGSVADDMSLGQYWEAVSNAVDY
                     ICPMMYPSHYANGTYGLPVPDAYPYETVFNSAKDSAARNKNIETPGAIRPWIQDFSAP
                     WVPGHIKYSDKQVKEQIRALEENGIGEYLLWNAGNRYSEGAVTE"
     gene            complement(68345..68875)
                     /locus_tag="PKU88_01040"
     CDS             complement(68345..68875)
                     /locus_tag="PKU88_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67908.1"
                     /translation="MTEYKGRWEAIRGSLIVTAALIALITCIYVLVPHGFWAYFFIGL
                     LLLFIPTAYSGLILKITMDEKKIVVVRPFTRVTVKFEDVALCAVNCVGDGKYLIYAFV
                     KQRYRGGYTVKGIKPKLPFDEVIKMSLKEECLDLDVNFNRAKKIPVSFVENGEELKDR
                     FLLEVGKHQVKIMKEV"
     gene            69115..70404
                     /gene="hcp"
                     /locus_tag="PKU88_01045"
                     /gene_synonym="priS"
     CDS             69115..70404
                     /gene="hcp"
                     /locus_tag="PKU88_01045"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPX67909.1"
                     /translation="MFCNQCEQTPHGGCTKVGVCGKNEDIAALQDTMIFGLKGIAAYA
                     THAREMGYIDPEVDGITHEALYMSLTNSNFNLKEHIDMVLKVGSAAVKVMELLDKAHT
                     ARLGVPVPVTVSQDKIQGRCILVTGHNLYALEELLKQTEGKGINIYTHSEMLPAHGYP
                     ELKKYSHLKGNAGKAWYDQRKLFEEFPGAILGTTNCLMPIRGTYSDKFFSYGTAGLEG
                     VQKIINDDFSPIIEKALSLPEVDIESDKTLITGFHHQTVLSIAPEIIDAVKTGKIRRF
                     FVIAGCDAPTKGRDYYRELALSLPSDCVLLTTSCGKFRFNDHDFGTVPGTNIPRYIDL
                     GQCNNSGSAAKIAIALAEAFNCSVNDLPLSIVLSWFEQKAVAVLLGLLHLGVKNIHIG
                     PKAPEWFTSNVAEVLQKNFNLQLISGDAKADLEKMLG"
     gene            complement(70418..71041)
                     /locus_tag="PKU88_01050"
     CDS             complement(70418..71041)
                     /locus_tag="PKU88_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPX67910.1"
                     /translation="MEIIYTKTSLIAAQNSLELSRKGYELLDKKRNVLIMTMMSFIEK
                     AEEIQKKVEVVFGDAYEALQTANTTMGISNIEQIAQSIPEASGFEVLDKSIMGVEIPE
                     ISHQEEKLIHYYSFYHTNTALDVAMQKFHEVKLLLYELSEIEDSVYKLAMEVKRTQKR
                     TNALQNIQIPKYEEIVGKILEVLEEREREDFFRLKVVKKKGEKKAAK"
     gene            complement(71050..72423)
                     /locus_tag="PKU88_01055"
     CDS             complement(71050..72423)
                     /locus_tag="PKU88_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPX67911.1"
                     /translation="MRLKYLKLQKVEGPLIILSGAKEAVFGEVVDIEVEQEGHKKGKV
                     VQIDGDKVIIQVFESTSGISLNNASVSFTGKPMEIPLSRDILGRTFNGIGEPIDGAGF
                     YADSKYDVNGRPINPVGRRYPRNYIETGISSIDGLSTLVRGQKLPLFSGDGLPHNELA
                     AQIVKQARISGDNESNFAVVFAAMGIKHDEADFFRKQFDSSGVASRVVMYINLADDPI
                     VERITAPRCALTAAEHLAFVHNMHILVIMTNMTSYSEALRELSASREEVPSRKGYPGY
                     LYSDLASLYERAGMIKGSKGSITQIPILTMPNDDITHPVPDLTGFITEGQIILSRELH
                     QKAIFPPINVLPSLSRLQKESIGEGFTREDHPDVANQVFSAYSHVQDVRSLAQVIGED
                     DISEMDKRYMEFGRQFEQKFLNQGSEVNRSMEDTLDIMWELLTFLPKSELDRINPELL
                     DKYYGKR"
     gene            complement(72420..74204)
                     /locus_tag="PKU88_01060"
     CDS             complement(72420..74204)
                     /locus_tag="PKU88_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPX67912.1"
                     /translation="MDTNKGKIVYINGPVVKVAEANDLKMREMVLVGRRQLIGEVISV
                     DKETATIQVYEETTGLMRGEGIISTGRPLSVKLGPGIIKYIFDGIERPLLSMYEAGTA
                     FIPEGIGMITIDEEKLWDAEIIVGIGDYLDSGDIYAKVQETSLIEHRLMVPPGISGRV
                     VFAEKSGSYNIEHVLVKLETETGLHELKMYQMWPVRGARPVSRRNPIAKPLITGQRII
                     DSFFPLAKGGTAAIPGGFGTGKTITQHQLAKWSDADIIVYIGVGERGNEMTEVLEDFP
                     KIIDPKSGKSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYNIAIMADSTS
                     RWAEALREISGRLEEMPAEEGYPAYLPSRLAQFYERAGDVEALGGRDGSITIIGAVSP
                     AGGDFSEPVTQNTKRVVSTFLALDKSLAYARHYPAINWLKSYSGYITDLSSWYEENIA
                     PDMMELRAKLLRLLQEEEKLMEIVKLVGEDILPDDQRLILEISRVIKVGYLQQNAYHK
                     NDAHVSLKKQYGMLRVIDRLYDKAHNCVKMGAPISKVKNDELFYKITTMKYNVPEDKP
                     EMFDELINEIDNFYDSLKTMYGREVSRQ"
     gene            complement(74209..74835)
                     /locus_tag="PKU88_01065"
     CDS             complement(74209..74835)
                     /locus_tag="PKU88_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPX67913.1"
                     /translation="MSVSVEDKIELFRNIIFKDIEETAYDRRHKAAEGFNQNKDRLLQ
                     EVEIRKNQIMQEAEKKAEKEKQQLIAKTKSQGQHRLLEKRQQSINEITEMLIRKADSF
                     VSEEGYKEYLAASLKKAAVDFENAASVQFAFTKRDLRTLKGFIEQEIASGELKDRCQL
                     RESEKNIIGSFYAEDEKREIQADYTLRSLIEENRELIGSSISRRLDEV"
     gene            complement(74847..75155)
                     /locus_tag="PKU88_01070"
     CDS             complement(74847..75155)
                     /locus_tag="PKU88_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPX67914.1"
                     /translation="MRFFLLSDNIDTLTGMRLAGINGVLVHEREEILAAFDEACSSND
                     IGIILMTELLAEKVPEELKAIRLDASKPIITIIPDRHGERRRRDYITSYIKESIGLKI
                     "
     gene            complement(75195..75614)
                     /locus_tag="PKU88_01075"
     CDS             complement(75195..75614)
                     /locus_tag="PKU88_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPX67915.1"
                     /translation="MNTILGIAIGIVIFTIGLGVVLYRSAANIQGRKIRNILRASLVS
                     YMFLIALFLFILTPDIVNAAQEGDSGAGMGFIAAGISTGLATIGAGYAVGAVGSSALG
                     AVSENPRILGKTLIFVGMAEGIAIYGLIVSIMILGAL"
     gene            complement(75667..77655)
                     /locus_tag="PKU88_01080"
     CDS             complement(75667..77655)
                     /locus_tag="PKU88_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPX67916.1"
                     /translation="MAVERMKITSIIGKLTDLDKVSRLIVINGSTHILNAISELNSNN
                     LNLSVPQKHMQILQELVNIKPYISGRDFSGDEGIIRLFEDLFNLKLKICEDYLCVGDE
                     YENTMAELRKVHEDVRHISEDMAERAANIEKLQQYLNNVSYLRNTDLRLEEINNLNFM
                     KFRLLMLSNENFKKLKLNIENVPAIIFQAAVFNGNTVIASITPKSLEEEAERIFISLN
                     YKELELPQEFSGTIEDVINQINAKSSNEKRQVEELRESVAEMSRTYSDTVFKAYTLLE
                     LEKKAEKLKQETALGSSMFFVFGFVPESEVEGVRIELKQYFGESIIFIADDVEDKRLG
                     NAPPTKMKNISLFKPFESLISMYGMPSYGEKDPTPFFALSYMLMFGAMFGDMGQGLII
                     LLAGLLISYRTANKSFGGILTRLGVSSMIFGALYGSIFGSEELIGALLIRPMANINTM
                     LIGAIVLGIILITISYMYSLLNHYSSGNIEEGLFGREGLTGFLFFLTLVFGALGKATG
                     ELTVGIGLPIGIMVVLLLIMVFKQPLAGMLTGRRLYEDSPGDYYIEAGFGVIETILSV
                     ASNIISFIRVGAFALNHVGLYIAFAAMAEMLNSKTAGIMVLIIGNIFIIGLEGLIVFI
                     QSLRLEYYELFSKYYSGYGVEYKPVGLFGKGDSGNEMV"
     gene            complement(77672..78715)
                     /locus_tag="PKU88_01085"
     CDS             complement(77672..78715)
                     /locus_tag="PKU88_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPX67917.1"
                     /translation="MGNITRFAAVNTKIKSMEGELLKDEDYTLLLGLESVTEVAGYLK
                     ERTAYSEVLAEVDTGTIHRGMLENLIKQKMVKSIDRIIHYFTGDYKSFVKTLYAKYEI
                     EELKILARAVYNGKDAAAFRNNVFVGKHSRIDTSKIYEAKYIRDIIYALQGSEFYKFL
                     IPLVDGDFSENPFRFEMVMDMSYYSILQNKWSKLEKRDIKALEQAQGIIADLLNIQWI
                     YRGIKFYRLSPEELLNYTINIGYRMDFNMLKTLCYSKSLEEFNRIVKSTRYGFMLKHD
                     GTTDIYMERRMERYIYFELKSMMKNNALSIISAFAFIIFLQFEVKDIISIIEAIRYKV
                     PADQAYKYIVRKI"
     gene            complement(78721..79047)
                     /locus_tag="PKU88_01090"
     CDS             complement(78721..79047)
                     /locus_tag="PKU88_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67918.1"
                     /translation="MDKNIDVYIRRLIELDSRAVELKRERDAEISELEMRGENELKGL
                     DEELEEAVIKAKQRRDEIIEAGRLQAKEMEEAVNLKIDKLQTHFENFKEDAARDIWKQ
                     LLAIER"
     gene            79328..80902
                     /locus_tag="PKU88_01095"
     CDS             79328..80902
                     /locus_tag="PKU88_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPX67919.1"
                     /translation="MEIFHNEYFSLFSVNGELYICVYHTGYEIREFNSLLMDMPFLQL
                     NSFTTLKNALDEASRLRICIGKIKPRVEVEISDDEMEASIMLNISAKEFAENKVPISS
                     EIIEALNNAEVVFGHDNIFKKPITVQKKIKAAKGTKPEDGKDTVIKYYEIPDKKPIVK
                     EDGTVNHYELNLIDNVKKGDWLGEKIPATEGKPGITVTGKVLPARKGIDLTLKYDKKT
                     VEEHEEGGKIVLRAKTDGAVKFENDKIKVDNHLIITKDVDYETGNISFEGYVTVKGIV
                     KDGFSVTAKNDISIQGNMGLGVINKIISKEGSIYIKGGIFGKNISEIQAKKSVFIKYC
                     NECKIVAGEDINIGFYSLDSHLEAKKVILDPMHGKIIGGSVNAEIQVVTGIIGNRSEK
                     KTYISVSGFNREAIRKEIEQLSQKYSVTLNEAAKAKKQIESFEINIPGAEYVNSREYH
                     QCIRKYENILDEIKLLDEYRKKLERILETKGEGEIDVSKAAYPETYIQIKNMQKKIDS
                     IVKGSFYALDKKLHHN"
     gene            complement(81034..82272)
                     /gene="tyrS"
                     /locus_tag="PKU88_01100"
     CDS             complement(81034..82272)
                     /gene="tyrS"
                     /locus_tag="PKU88_01100"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPX67920.1"
                     /translation="MVPKYNVYDVLKERGFIAQVNHEEELRELLGKECITFYIGFDPT
                     ADSLHVGHFLQIMVMAHMQKAGHRPIALLGAGTAMVGDPSGKSDMRKMLTFEMIDHNA
                     RNFRKQFEKFLDFRNDRAIMDNNANWLLKLNYVDFLRDIGVHFSVNRMLSAECYKSRY
                     DKGLTFLEFNYMLMQSYDFLELYRKYGCRLQLGGDDQWSNIISGTDIIRRVEGADAYG
                     MTFTLLTTSEGKKMGKTESGAIWLDESKTSPYEFYQYWRNVDDADVEKCLALLTFLPM
                     DEVRRLGSLPGERINEAKKVLAYELTRMVHGEKEAAKAQNTAESLFGGSGDSEHIPTT
                     ELSKEQLDKGVNIVDLLVMTGLAPSKGEGRRLINQGGVLVEDTRVESIDLIIGTANLK
                     DGKLIIKKGKKTYHRIKIKE"
     misc_binding    complement(82318..82596)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            82742..83617
                     /locus_tag="PKU88_01105"
     CDS             82742..83617
                     /locus_tag="PKU88_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX67921.1"
                     /translation="MKKSLKADLALILVTIFWGAGFPATKIALQTMTPFYHIGIRFAV
                     ASLLLSLLFYRKLRSLNKSLIKPALILSSLLFATYAFQTVGIQYTTASKSGFFSGLAV
                     LIVPIFSIFYLKTKLELKTIISVATATLGLFLLSYTGSDFNFNIGDFLTILCSICYAW
                     QLLFTGTYVQKHDATLLAIVQLFFVSLYGMAFAVILEPIPVNMSVPSFWSLMFSAVFC
                     TAFAFWMQTTAQKFTAASHIALIFTMEPVFGALTSFLLLNEVLGARGIIGGILIVSAM
                     IISEFQWAKSQSTSS"
     gene            complement(83604..84122)
                     /locus_tag="PKU88_01110"
     CDS             complement(83604..84122)
                     /locus_tag="PKU88_01110"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPX67922.1"
                     /translation="MSILIQALLIFFARIIDVSIGTMRTILLVKGQRRIASVLGFFEV
                     MIYLIVLGKVVGNIDKPMLILAYCSGYAAGNIIGSKIEERLSIGRLVVQVIVKEALEE
                     LVESLREAGFGVTIIEGEGKEGKSYMLNIILDRKQVKTLYEIVDGCDCGAFITTMDVR
                     SSLGGYFQPKKM"
     gene            84325..85269
                     /locus_tag="PKU88_01115"
     CDS             84325..85269
                     /locus_tag="PKU88_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPX67923.1"
                     /translation="MKKYKALFSEAAILLLLVSSLGGCTYDPARRYIQTDPNTNNMAA
                     PNTPAIPNNVVGRADNNADGNKISATYEVVDFSYITCNTNNLNVRAGAGNKFPSVGTL
                     RSGQKIRALGKLDGWYVVKMPDSGRIGCIPSASARPYGTSANTGTTGAGTVTPSPNQG
                     AITGAGAGAGTTGAGTTGGGTAAGSGAMSSDESRILQLVNAERAKAGAKALSSSSDCT
                     RLARMKSQDMADNNYFSHQSPTYGSPFDMLKSNNVSYMYAGENIAMNQSAEAAFKAWM
                     NSEGHRKNILNPNFTELGVGIAPKGNGSYIYTQLFIGR"
     gene            complement(85305..>85469)
                     /locus_tag="PKU88_01120"
     CDS             complement(85305..>85469)
                     /locus_tag="PKU88_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPX67924.1"
                     /translation="LPLEGLALVAGIDRILDMARTTINITGDSACAVIIAASENELDR
                     DCQIKHTYSA"
BASE COUNT        22886 a        20408 c        15182 g        26793 t
ORIGIN      
        1 aatcctcctg gtaattatac ccatataata ccaaaaggat taatctctgt caatatctgt
       61 ctatctgtcg tgcctggcac ctattcttca cctattcttc catcgtcacc tcttaacgat
      121 aatttcatag gttcttgatt gaacctgact tctttcaccg ataaatacgg tcaatctcgt
      181 ggttccttcc ttattgcctg taactgatgc actgtaaccc gatataatga cggtcttttc
      241 cgcaacttca ccaagtatcc agttagtata ccctcccaca atatcgccgg atatgcttgc
      301 tatactgtta tcatccacct tccatgtcgt aatcttttct gaatttgaaa aatcactgcc
      361 gcggacgata acattaatgt ttattgtctg tccggcagaa acaataatgg gtgattgctg
      421 gaccagcctg aattcgtcca gttgatattt atcaatattc tcatacattc ttttggcaag
      481 aataattgcc tgctccctgg tcaaagcacc tttaggtgtc atttgattat tggacccctg
      541 aatgattcca cggtaattga tatagtaaac tgcatccacc gcccagtttg agatattagt
      601 gtgatcctca aacattttgg gggaagaaac atgtgtatct aatcccggtt ttattttatt
      661 cataagtctg aaaaacatca atgaagcctg ttcacgggtt aaagagtcct tcggcataaa
      721 catattattt cctgctcccg taacaattcc aagctcgcag gctttcaaaa catagagatt
      781 aacagtatcg ttgaagggat ttgcctcggg gagtggcgct tcatgtttcg tcaggatttc
      841 ataaagtcga accaccagtt cactaaaatc ctcccgtgtt atattttcgg tgtagctacc
      901 cagcaagtta ttagaggtaa ggcccaaagt tttggcttct tccacatctg tctgtgccca
      961 ggagctggga taattacctg cataggtatc caatttttcg ttccatccca gttttgcgat
     1021 ccatgcgtca tatgaatagg attttgtagg attgtgaagt ccttttacgt ccttatcatt
     1081 agaattggta tacccggcta ctatatatcc atccttctct gctaccaagc tttttggttc
     1141 atccgcattg cttccgccca agcttttttc ccatatcaat ataccctcgg aggacagacc
     1201 ggtcaaccag aaatccgagt tcccttttga atcgctgata tctccgtctc tggattcact
     1261 gcgtgcaagt atagcataat agccgtcact tgtctgttta atgcttatca catgctcatt
     1321 ccggctcccg ccaagatagt ttgcccactg gggattctta ccgctatcaa atttaacaac
     1381 cataataatc ggactggtgt tatatgtgtc agaatacaga gccatgggga aactccgttt
     1441 cgtgacgctc ttatataggt caatcagttc ccggtgatat tggttcaccg acaccgccca
     1501 aacaaatccg ccatctgcgg ttccttcaat gctttgttct tcctttacag agggctcctg
     1561 ccagagaatg tccccatcct gtgtgaaccg gacaataaaa tcaccggcag agataaaatc
     1621 cccgttattc agctgcgcta tatcatataa gtaatatccg ggcagggttt tttgccacct
     1681 tatacttccc tgaaaatcca gcctaaccgc atatgcatta taaaaattat tttttccgcc
     1741 ttgaatcccc atcaggtacc catcgtcgat aatgtgaaga cttatcactt tacttgcttc
     1801 cgtgtccaca tcctgcaata ctttttccca tagaatatca cccttggcag acagttttac
     1861 catccagacc acaaagtctg aggacggatt gttttgatta ctgacaacat ctccgttttt
     1921 cgaaaatgtc atccctgcca gtataaaggt tccatcactg tgctgccgta cagcaaaccc
     1981 catatcacct gcggaaccac caagggtttg atgccatacg ggctgtccgt tggaatcaaa
     2041 ttttacaacc agcaaatcat catcttcacg aaagccggag ccgggctcat tcacccgctg
     2101 ctttgttgtt atatctccat ccggagaatt ggttgtgcct atagccagat aaccaccgtc
     2161 tgaagtctgt atcacttcag atatttcggt gttatcactg ccgccgaaag ccttttgcca
     2221 ctgaatctga ggcgactggt gaacctgagc atgaacagtt aaaggaatta aagttgttgt
     2281 catgtaaact aaagccaata acaaaagttt tatttttact ttcatggtta accacccttt
     2341 catagtatga ttttattcat attatctgtg atatttttca aacatcctgt tggccagtat
     2401 aaaagcttgc tctcttgttg cggttccttt gggattaaaa ccggttcccg tacccaggat
     2461 aaccttttcg ccattcatca ctgtaacccc cttaaccgcc cagtcagaaa tctcgttatt
     2521 atcatagaac ctgatttcat gtgatgtttt taactgaata tccggcctcg aaggcaggat
     2581 tgcttccaga atcctataaa acatcaccgc aatctgttcc cttgatatgt tttcatttgg
     2641 agcaaaacgg ccatttccta caccctgaat aattttcaaa ttgtatgatt ttaagacgct
     2701 gatatcattt gtatccgaaa aaggagttac catatcatag gttgctttcc ggccactaag
     2761 ctcttcatac agcttaacag ccagtgtgca aaactcctgc ctggttatat cattttgata
     2821 ctgactgagc aagctctcat ccaccaggtt gttccgaatt gctctggtta cttcttcctc
     2881 agcccagaaa cttggtttat cggaaatatc accttcagca tcttcttccg gaacttcata
     2941 ccgaataaca taaggctgat attccgttcc cggccctgac atcgaataac ccggtttcaa
     3001 gcacagtgca ggtctgacgc cattagcctg gtatgcattg aacccgtgtg gatcacttaa
     3061 tttgtttgat ggaattgtca tgacagacgc tgcgtagtca taatcgggtg tccgcagcca
     3121 ataggtattg atttgccagc cattgggggc agtataccag ggtgctgttc gattcaattc
     3181 gttttcttcg atccaggctt ttacttcatc aacagacaac aaaaatattt tgtggcttga
     3241 caatttataa taggcattac aattgtctcc attgagattc catggctgat tcccaccatc
     3301 cttcaccggc aaatcaaact cactcagata tgtacgaagc gatattgtct gtattgccgc
     3361 catttccata tcatgaaagt ttgtaagaaa acccggctca tccgcatagc tcacatcctc
     3421 tgccaatata ctgctcttac cgtttacaaa ggttgaatac gataccccca tagtattggc
     3481 agcatcaggg ttttgatgtg aataattcac tgtctgttcc tcgctgttca accattcaca
     3541 taagtttgcc ttcgtccaat agttgctgcc ttccgccact ctggtattgt acttgctgtc
     3601 tcggccttct gccctgtctc cgctggcatc aaaagctttt accgtaataa tatcctttgc
     3661 aaacaagata ctcccgtttt catcattatg tacaaccatc catacaatag gtttgtcttt
     3721 atacatgcca aactgcacat aacttcctat cggcagcgaa cccgaaaaag ttattgcata
     3781 tacttctagc agaccgccca aaattattat catactcatg gccagggtaa aaactatttt
     3841 cccccaatgc ttatacctga taaacaaatt tatgcatttc attttaatca cctcatacag
     3901 tattttatat actatgtaaa accggttcac tatcaatcat ctgtgttagt ttaattataa
     3961 caagacaaac cttataacag tctgcagcct atattttgtc aatgacaaaa tgagtcacaa
     4021 atcatgacat aattctgcat gaatcgcatg ttaactgcca ttaaaccttg ttgttagata
     4081 atctctgtgg tatatttata cagaggtgat tttcttggaa tttcatgaca aattaaactt
     4141 tttgatggat ataacaaaaa caacaaacat cagcctggca agggcctcat caatggatcc
     4201 ttcctatatc agccggctgc gtaacggaaa aaggaaacct gtaaaaaatg cctcttatat
     4261 gatagtcttt gcccgttttt tttccaaaag gatacactca gaaaaacaaa tggatgtttt
     4321 tagggaggta ttgctgccat acaccatgtt tcctgaaagc gagagtgctt taacggattt
     4381 cctgcatcat tggcttgtga ataatgactt taaaaacggg cacctgaacc tgcctgttct
     4441 gcataggctt actgatgcag aaccaagagg cggcttcaat aaaaatttga atgaaaatgt
     4501 gaagccattt ttcaaaaccg cctgcggtct ttaccagggt atacaggaaa ggcggctggc
     4561 tgcggttcac tttctgagca caattctcaa ccagaattct ccgagaaccc tgtttttttt
     4621 cagtgatgaa gatatgagct ggatgactgg cgatccggta ttctatcaaa agtggtctca
     4681 tttgatgggt caaatcatcg ccagaggaaa tcggataaaa atcattcatg atttgacccg
     4741 tggcctcgat gaaatactgt cctcaataca gctctggatt cccctctatt tgtccggagc
     4801 catcgaacct tattattatc ccaggaaacg agacggcata tataaaagat cactgtacat
     4861 tgcccccggc ttgtgtgcag tttcttccga ctctatcgga acgatgacgg ctgatgaatc
     4921 caccgcggtt tttcaccagg accttagaat ggtagaagcc cttgagaatg aattccatca
     4981 ttatctcaac ctatgcagac cccttatgag cattttcact ccggataagc atcagaatta
     5041 tctgtccctt cttgaatctt ttgaaaggga gtacgctcct accatattca aaaccccgca
     5101 tctttccatg ctctccatgt ccgaatcctt aatggaatcc attcttttac gatattgtaa
     5161 gacaagctat aataaaaaca gaagttttta caataaaaga ttggaaaatt tccgaaaaac
     5221 attgaaaaga cacagcttta cggaaatttt cagtattccg gaaaaagaag ctctgctggc
     5281 aggcaaagct agaatcaacc tgccaaatcc ctttgaatcc attgaaggtt tctacacccc
     5341 tgatgattac cgtcgtcatt tagatcatgt ggcagtcctg actaagcact atgacaacta
     5401 caatgtctgc attgaaaaaa acgaatgcag tctgggatac actctttatg cgaaagaagg
     5461 tataggcgtt atagtagcta aaaccaaaac ccctgctgtt gttcttttta ttcaggaaag
     5521 caatttattt gccgggtttt gggatcttct gattgaaatg aaagagcatg gtgccagaca
     5581 cgatgtttag acagttatac tcatctgctt tggaatacat aacctaccct cttgtatttt
     5641 acattaaaaa tacttgctta ataattattg atttgatgta ttatttcctg aagctttttt
     5701 tcaatagtgg ctggcactcg ttcgacctgc ataaaagcct tccctgatag cattaccgac
     5761 ctttccaacc tgctgacagt caccgattat ccgagtcttt gtatggaggt tatgataata
     5821 aaaaatacga ccgcagtcgt attttttata ttattttaag tttttgacaa acttttcgat
     5881 atttgcgttc tttctcattt cttcgagaag cttctcatat tccgagccct tcttctcata
     5941 aagcagtttc cccttaagca tttcttttac atcctcaaat ttgtcccgct tactgtcctc
     6001 aacttttata atgtgaaaac caaagtcagt ttgaacgatt tcactgatct ctccgggctt
     6061 tagtgcaaaa gccgctttct caaaaggctc caccatatct ccgtgcctga agtagtcaag
     6121 atctccgctg ttttcctttg cactgggatc ctttgaatac tgctttgcca actcggcaaa
     6181 attctctccg gcttttaccc ttgtaagcat tttctctgct tcttccttgg tatccagcag
     6241 tatatggctt gccttgacgt tcataaattc atcctgatgg gtatcataat aattttttag
     6301 ctcttcatca gtaactgtta tatcctcagt aagcttttca acaagcttat ttatggtaag
     6361 ctcctttttc accgattcct tgaagtattc aagatcaata ccctgcccct tcagaaattc
     6421 atttaacttt tcctctgaat cgaagtattt tctggcattt tcaatttctg cgttcacttc
     6481 gtcatcggta acggtaatcc caagctccgc agctttttcc atctgaagct tttcatcaat
     6541 cagcatctcc agaagctttt ccctggcaac atcgccgaac ttccttcctt caacctcctg
     6601 gtcccacact tcagtaccaa actgctgctc gtactgtgtc ttgaatatat caaacatttt
     6661 attaaattct tcttttaaaa tttctttacc ggaaaccttt gcaactacag ttttattatc
     6721 aacttcggga tttactctga ccatattgca ggcagtcgga aatactgtca gcaatacaag
     6781 tgccaaagat accagaagca gcctgcattt tttattaaac aatttacatc gctcccctac
     6841 ttgtttttag tcaaggttaa ttatacccta tatcagccgt tctgaaaact actaattttt
     6901 cctagaaatt ctttcaggaa tttgatatac tcatccggtc tgtcttcatc aacttttatg
     6961 gtgaaatatg gctggccgct tgcggtaaaa agcagcctgt cgatgtattc ccctgctacc
     7021 cttatagctg tctcaggctt aatgctcctg tcgctgctga atttcaccaa tatgctgttt
     7081 ttcttttgag ttatggatac tacattacac ttacctgcta aatgctttat ataggatata
     7141 tccagcagat tcctgactac ctgcggtaaa tctccgaacc tgtcctctat ttcctcttca
     7201 atgtcataga tgtcctcctg gcttccaatg tatgcaatct tcttataaat ctcaattttt
     7261 tgattctcat cggatatata gctttccgga ataaatgcgc taatcttaag ctctattgat
     7321 gtatcaacgg tctcggtgac ctcttcgccc ttaagctcct ttaccgtatc ctcaagcagc
     7381 tttatataaa ggtcatagcc tatggcctcc aaatgcccat gctgctcccg gccgagcaag
     7441 ctgcctgccc ctctgatttc caaatccctc atggctattt taaaaccgga accgaattcg
     7501 gtgaactccc ttatggcttg aagccttttt tccgcaactt cattcagcac tttatccttc
     7561 tgatatgtca gatatgcata agcaagcctg ttggaacgcc caacccttcc cctgagctga
     7621 taaagctgtg ataatcccat tttatccgca tcacttatta ttatggtgtt tacattgggt
     7681 atatccaggc ctgcttctat aatagttgta cagaccagta cgtcgtattc attattataa
     7741 aaatccagca tggtgtcctc aagcatcttc tcttccatct gcccatgggc taccgccacc
     7801 cttgcatcgg gcagcatcgc cttaagcttt gcggcaattt tctgtatgct tcttacacga
     7861 ttgtgcaaat aatatacctg gccccctctt gctatttctt tggcaattgc gtctttgatt
     7921 acctcttcgt tatactccat tacataggtc tgaacgggat acctttcttc cggaggctct
     7981 tcaattatgc ttatgtccct tatgcctgaa agggacatgt gcagcgttct tggaatggga
     8041 gtcgcagtca gcgtcagcac atccacattc tttttcaggc tctttatctt ttccttatgg
     8101 gcaacgccaa acctctgctc ttcatcaact atcaaaagcc ccaaatccct gaactccacg
     8161 tcattctgca gcagcctgtg ggttccgatt attatatcca cattgccgtt ctttatattt
     8221 tggattatcc tgctttgctc agcatgagtc ttgaatctgg ataaaacgtc tatattcaca
     8281 gggaagttgg caaacctttg aatacaggta ttataatgct gctgcgccag tattgtcgta
     8341 ggaaccagga tcgccgcctg cttgttgtcc atgacgcatt taaatatcgc ccttagagca
     8401 acctcagtct taccgtatcc cacatccccg cacaacagcc tatccattac tcttgaatct
     8461 tccatgttct tcttgatttc ttcaatgcat ctcagctgat cctgagtctc ctgatattgg
     8521 aacatatcct caaattcttt ctgccatctg gtatcctctg aaaactcgaa gcctaccgcc
     8581 tgctgccttt ctgcataaag cttcagaagg tccattgcca tagcttctat ggccttcttg
     8641 gcttttgcct taacctttac ccactctgtt ccgcccagtt tgttcagctt ggggcccttg
     8701 tcctccgctc ctatatattt ctgaatcata tccagctggt ccgtgggtat ataaagcttg
     8761 tcattccccg aatacctgat atgaaggtaa tctctggtag tgttatctat cttcagtttc
     8821 tctatcccaa tgtactgtcc tataccgtga ttctcgtgaa cgacaaaatc ccccgctttg
     8881 agatcggtaa acacatttat ggctttgcct tttttctttt caatcttctt cctgctcttt
     8941 gagccgaaag gctctctgtc gcttatgacg gcatacttta tcgatggaaa atcaaatccg
     9001 ctgttcagta ttccgggaag aatgattaca tgcccttcct taagctcaat atcaagctca
     9061 tccttgtata ctgcatcaat atccctgtct ctaaggtctg aagccagcct caagcccctg
     9121 tcccttgagc ctgaaagtat cagaaccctg tatctattct tcctccatac ggcaatctct
     9181 tccataagca tggaaagctt gccgtggaag ggatgcattg accggctggt aatgcttatg
     9241 gttttttgag gaataaaatc actgctgctt ttatacaatg aatccaggca aactgtcttt
     9301 ttctcccgga tcttcggaac aacatcttcg taagtaaata atgcttcaaa ttgccccggt
     9361 aaaacttcac ccttctccag aagctgatta aaatgctcca tgaattccgt ccctatggcc
     9421 tcgcagcgct gttttatcct gttaggctca tctatgaaaa taacacagtc ccccatgtaa
     9481 tctataagac tgtaattctt atctgaaaaa tacggcgaat atttttctat tccctcaaag
     9541 tgtataccct gcctcatttt ttcaatatcc tcggatatct tttcccttac cctgatatcg
     9601 gaggattttc tcctgcgctc atttcctgat gccctaagcc ttgcagaaag ctctgactga
     9661 agcttttccg ccccctcaag gaattcctcc tccttaacga taaattctct ggcaggcgta
     9721 attttgactt tttttgtctt gcttatagag cgctgggtca gcacgtcaaa gtatcggatg
     9781 gaatctatct catcatcaaa aagttcaatt cttactgcgt tctggtgtat gggagaaaag
     9841 aagtcaatta tacctcctct tattgagaac tgtcctttgc cctctaccat ttcaattcgc
     9901 tcatatcctg cttttatgaa atattccttg atctcatcca gtgagacggt ttgccccact
     9961 gaaagttccc tggtcattga tgcaaaaagc tcaggagaca ctgtccttgt gagcagtgcc
    10021 tctatggaag tgcagacaat gcaattttta ccgctgatga tatcgtcata tgcccttaat
    10081 cggttctgca ggatttcatt gcttctggca tcaatcctgt gaaatactaa ttccctgccg
    10141 ggcattagcg ccgccatatc cggttcaaaa aaagaaatgt cctcataaat ctttctggaa
    10201 agtaaatcat tgtaagttat gaaaagacat gcctttccaa aatgcctgca aacaaatgcg
    10261 gctgccgcag ccttctgagt atcggaaata ccatagactt ctactgtatt cagatttgct
    10321 tcaagagatg aaagcagttc gttgaactct tccatttcat aaagaggctt aagcacgttg
    10381 ccaatgctca agttatcacc tcctgagcca gaatcaataa tgtcagaaaa aaaaggcttc
    10441 tgttgaagcg ttgccttcat ttttccctat tataccagtt cattgccgag tttattccat
    10501 tttttattat ttcctcgata gccagagacg aattcttgac agcctcttcc atcaggggta
    10561 tttcctcatc tgaaaacctg ctcaatacat aatcagccaa atccataaaa tcaggctgtt
    10621 tgccaatacc aaccctaatc ctggggaact ggtccgtttg taataaatat attatcgact
    10681 tcattccgtt atgagtgcct gagcttcctt cagtccttat cctgattctt ccgatatcaa
    10741 tatccacatc atcatatacc actatgagct tggatatgtc acacttgtac cagttaacaa
    10801 gctcatatat gctttctccg ctcaggttca taaatgtctg gggctttgcc agtacaagct
    10861 tttcctctcc tatcatgcct tctcccaata atgccttgtg ctttatcctg ctgatgctga
    10921 tattatggac agaagcaaaa taatccagta ccataaagcc cacattatgc cttgtatatt
    10981 tatatttgtc tccgggattt cccagtcctg caatcagata cataataacc tccgcaatga
    11041 ttgctgcaaa atatttttag tcaaacagct tgctcaagga caatccttca tagattctct
    11101 tgatagcttc agcaaacaaa ggcgcaacag acaatacctt tatcttgtct acaggtttgc
    11161 cggcaagaag aggtatcgta tttgtaacaa taagctcctt tatcactgaa tccctgatgc
    11221 gttcaaaggc aggccccgac aaaaccggat gcgtacaggc aacatagata tccctggcgc
    11281 ccatttcctt cattaccttt gcagcattca ccatggtacc tgccgtatct accatatcgt
    11341 ctattattat acagttttta ttctcaatat ctcctatgat attcataact tccgacacat
    11401 tcggtttcgg ccttctctta tcaattattg ccagcggagt atcaagcttt gtggcaaggg
    11461 atcgcgccct tgtgactcct ccgacatccg gagaaaccac aactatatcg tcaagcttct
    11521 tgttgatgaa atagtttaca ataagggggg ctgcatacag atgatccacg gggatgtcaa
    11581 aaaagccctg aatctgcggt gcatgaagat ccattgtaat tactctgtct gcccctgcgg
    11641 cttctataag gttggcaacc agctttgcgg taatgggttc tctggacttg gacttcctgt
    11701 cctgccttgc atagccgtag aatggcatta ctgcattaat cctcccggcg gaagctctct
    11761 tcaatgcatc tatcattatt aagagttcca tgagattatc atttacggga gtgcatgtgg
    11821 actgaaccac aaatacgtct gcgcccctaa tggactcatt tatacttact ccgatctccc
    11881 cattgctaaa agtacttacc tgagcatctc ccacgggtat gccaagatta aaggctatat
    11941 ccttagctaa gtctttattg gcattacaat ttaggacttt aatgctcttc ccatgtacat
    12001 tcatttcaac agaaacctcc caaaaatatt atttgttttt tcttttagtt acccaaccct
    12061 ctttattttc ctgccttgcc ctgccaatgg ccaggctgtc ctgaggcaca tcttttgtca
    12121 cagtagtacc ggctgcaaca tatgtgtttt catttaccct taccggtgca accaggttga
    12181 cattgcagcc gacaaaggaa ttatccccta catatgtccg gtacttgttc ttcccgtcat
    12241 agttgacaaa tacgactccg cacccaaggt ttacattgct gccgacatcg ccgtcgccta
    12301 cataggttag gtgtgaagct ttcgaattgt caccaaaggt ggaattcttc atttcgacaa
    12361 aatcaccgac ttttgcatgc tttccaattt tgttgcccgg tctcagatat gcaaaaggac
    12421 cgatagaagt gccttctcct atagagcttt ccaacactac cgagttctgt atctttactc
    12481 ctctgtctat ttttgcatcc ttaatccttg aataagggcc tatcacagag tcctcctgta
    12541 taaccgtatt cccctcaatt atgcatccgg gatatattgt aacatccttc tcaactgtta
    12601 ccgaagcgtc tatatatgta ctgtccggat caattattgt cactccctcc agcataagtt
    12661 tcttaacaat cctgcgctgc ataattttgt tggcctcgct cagttgatac ctgttattta
    12721 cccccaatat ctcttcggca ttttttattt taaacgcccc gatcttaagt cccttttttc
    12781 tcattattcc aataacatca gtaatatagt attctccctg ggtattatca tttttcaaat
    12841 accccagcgc ttcctgcaac tcttttccgt caaaaaagta tatccccgag ttgatttcct
    12901 ttattagctt agtactgctg tcagcgtcct tatcctctac aattgcttca ataagcttct
    12961 ctccgattct gattatcctt ccataccctg cagggtcatc catatcagaa gtcagtattg
    13021 ttgcactgca gccctcagcc ctgtgcatct cacgcatctc tagaagggta tcgggccgaa
    13081 gcagcggagt atctccatac aatataagta tatcgccgtc attaatgtat ttttctgcct
    13141 gcataacagc atggccggta ccaagctgcc tttcctgcat aacaaattta acaccggtga
    13201 cttccttgcg aacctgttcc gctccgtgtc ctatcactac aacaggctct gcaccggaaa
    13261 cctcccgggc acagttaatt acatgctcta ccattggtaa cccgcatact ttgtgtaata
    13321 ccttgggtac tttagagtac atcctcttgc cctctccggc ggcaagtatt attggcgtaa
    13381 tactgtatac attactcaat ctatcacctc aatcactagt taattacaat actattttat
    13441 cataacgaaa cctcaggaaa aagaaccaat aggaaataca ataaaatagc tttagtttag
    13501 tttgcttaat aaaaggcaaa ccattccaaa gctgatttac tacaaaaatc ttaagcttat
    13561 gtccattgcc ttaacagaat ttgtcagcgc ccctgcagat attatgtcta ctcctgtttc
    13621 tgcgactgct cttacgcttc tttctccccc gattgtaata ttacctgaag cttccagcag
    13681 caccttaccg gatgcaatgc ttacagcctc cttcatcata tccagtgtca tgttatccag
    13741 cataacaatg tcggcaccgg cttcaatggc ttctataagc tctcttattg attccacttc
    13801 cacttctatt ttcagcccat gagagccctt cgctcttgct gcggcaacgg cgggagttat
    13861 accccctgcg gcttttatat gattatcctt tattagtacc atatcagaaa gattgaacct
    13921 atggttgaaa ccccctccgg cctttacgga atatttatcc agaattctga gccccggtgc
    13981 agtttttcgc gtgtccgcaa tcctggtttt caacccggag atttcagaaa catatttgga
    14041 agtcattgta gctatccctg acatcctttg gaaaaaattc aaagccgttc tctcaccctt
    14101 taatatgctt cttgcattac cgcttatttc tgctataata tctcctttga aggctctctg
    14161 cccatccttc atgcgggctt ggaagtcaat ggttccgtct accatttcaa atacccgcct
    14221 gcatacttct atgccgcaga taatcccctc ttcctttgca ataaagttcc ccctgataag
    14281 agccgtatcc ggtatggtag agttagttgt aacatccccc cagcccaaat cctcccggag
    14341 agcattttta attattctat caatgataat attatccagc acgggacatc ctcctactca
    14401 ctgtctgttc tgtaatggct gccgcaattt ttcttccttg caatggctcc tttcagtatg
    14461 agagatgcaa tatatgcaat attaagtgtc tccatagcac ttacgcttcc catttctgca
    14521 tcttcaaggc tgccgattat ttcattaatc cggttcaatg atttttccat gtccggtccg
    14581 ttcctttcaa ttccggcatg ggcatccatc agggttttaa tttcgtttct gatatccatt
    14641 acattcagag tttttcctga agctccgtaa acatttgaaa catgcacttc ttcaagctta
    14701 atatctttaa tgccggcatt aatatactct gcacttcttc ttccaaatac aagtccctcc
    14761 agcagggagt tgcttgccag cctattagcc ccgtgtactc ctgtatttgc tgcttcaccg
    14821 caggcatgaa ggcactttat atttgtcctt cccaacagat cggtttgtat gcctcccata
    14881 aaatagtgct gtacgggaga aaccggtata tagtcctttg taatatccac tccgtgctcc
    14941 agacagctct tataaattgt gggaaatctc tttttcaaat agtcggaatc cttatgggtt
    15001 atatcaagat atacatattc gctgcctgtt tttttcattt cggaaaatat agcccttgat
    15061 acaatatccc tgggagcaat ctctcccatt tcattatatt tatacataaa tctctcatga
    15121 ttcttgttca ataatatgcc gccttcgcct ctgaccgctt cagaaatcag gaaacggttc
    15181 cctgtatatt caggtgtata aaaggctgta gggtggaact gaacaaattc catatcggaa
    15241 agcaccgctc ccgccctgta tgccatggaa attccgtccc ccgtaaccac ctctgaattg
    15301 gtagtgtttc tgtatacctg tcctacacct ccgctggcgc atataacact gcggcagtac
    15361 agcaccttct ttgttgcctc ggtatcaatt cctgtcgcaa ccaatacacc tatgcatctt
    15421 ccttctgaaa cgaggagatc tatggcaaag caattctctt taatcgtgat attttcccta
    15481 tccctgcaaa ccctgagaag cgaatcgaca acctccttgc ctgtagcatc cccaagagca
    15541 tgtacaattc tgaaacgccc gtgccctcct tcccttgttc tggtaagggt accgtccggt
    15601 tctctgtcaa agttcgtccc cagcttctca agcagcttga tatcctttgg agcttcttca
    15661 acaagtacgg ttactgcttc ctcattgcag tggcctgcac cggctttgat tgtatccata
    15721 aaatggtact ccggcatatc atcctcgccg atagccgcgg caatccctcc ctgagccaga
    15781 taggagttgt tttcatccat tgtctccttg ctcaggacac atactttata gccgggatta
    15841 agattaacag ctgaatagag tccggcaaca ccgctgccaa ttaccaccac atcccacttt
    15901 tcgctttcaa tttttcgggt gtcaaaactt gttaagtatc tcagcggcat aaatatcact
    15961 ccttatttta tcttcagcat tctttccaaa gatttcaaag cttttatcct tatatcctca
    16021 tcaacatgta tttcgtgctg catattcaga agcgcgctgt gaacatcatc cagccttgtc
    16081 tttttcatat ttgtacatat caatcccgtc gaaaggagat aaaaattctt gtccggattc
    16141 tgcctcttaa gcaaatggag aatcccttcc tccgttccga tgattacatc tttttcgggt
    16201 atctctcctg catatttgat tattgccgat gtacttccaa ggaagtcggc aagcttctga
    16261 acctcaggct cacactcagg atgcatcagt atctttgcat tgggatgaag ctgtcttatc
    16321 ttttgcactt cctcaacctt aactcttttg tgagttatac aaaagccctt ccagagaatt
    16381 atttccttgt tctttacttt tgatgccaca aaactaccaa gatttttatc cggtacaaaa
    16441 atcacttgtt tgttttccag gctctctact accttcaaag catttgaaga ggtacagcaa
    16501 acatcgctct cagccttcac ctcggcggag gaattcacat aacataccac cgctgcatca
    16561 ggatacctgg ctttcatttc tctgagcttt ggcgctgtga ccatatctgc cataggacat
    16621 ccggcatccc ttacaggaag caggactgtt ttgtccggtg acagtatttt agcactttct
    16681 gccatgaaat gcactccgca gaatactatt acgtcactgt ccgtttcagc cgcatgctgg
    16741 cttaaggaaa acgagtcccc aacaacgtcg gcaacatcct gaacttcagg cacctgatag
    16801 ttatgtgcca gtattattgc attcctctct tttttcagcc tttgtatttc ttcagccaaa
    16861 ttgttttgca tctcaaccta ccttctttat tttacaatta tttacttaag tggtgcgggc
    16921 aataaaatca atatttttta ttgtatagaa aagttatatt tcccaataca ataaaaattg
    16981 atattcttag ttgaatatat cacttattat gccctttttc aacaatgtat aaaaaagata
    17041 gaatgaatat cattctatct tttaaatgga ttaccacctc ttatgctttt ttttgtaagg
    17101 ctttacagga tagaagggcg gccagtaaaa cggatatgga ctgtatttat gttttttctt
    17161 atgcttccaa tagacagggt attcatcaga ctcatcactg ctgtaatcac tgtcatcttc
    17221 ttcataccag tcaggcatcc tcatatcctc catcccctgc cattctccat ggtgcatcat
    17281 ggggcacatc atcgggcata tcatcggaca gccatgcatc ggcatttcca tctccggaat
    17341 ctccatatca tgcatatcca tctccggctt ctcagccttg ggatattgtt ttttcttgtc
    17401 ggtcattctt ttaccccctt cggattttaa tattaagctt tattaatata tacaatgaag
    17461 ggtatcaaaa aagaccgctt aaagaaaaca tccacctatg ctctaggtgg atatctatag
    17521 tccgtattac tcgttcttta cttttccata ttcctccaat attgaggttt gcagtctctc
    17581 tcttgttgaa gaatttatcg gatgagctat atccttaaat tctccgtcgg gagtctttct
    17641 gctcggcata gctatgaaca atccttcctg gccttctata acctttatgt catgaacaac
    17701 aaattcatta tcaaaggtta ctgagactac tgccttcatc ttcccctctg cattaacctt
    17761 tctgattctt acgtctgtta tttccattct agttcaccac cttcccgtcg ttcgttttgt
    17821 catttcaata ataattcgtc atatattagt gatttccttc tttttctaac aaacaatttt
    17881 gcaaataata caggaaacgg tatgtttatc ctgcttttat ctttaagtat ctttcatgaa
    17941 atatcaattg cggtaaatgt tttgcactga ataaattgtt tatagtattg tttgtagttt
    18001 tactttttta aatctatcat aaatttttca atttttagtg agacatgggg acgtttctta
    18061 aaaattagat gttacaatgt actagtaaca aaaaattggc tataataata atatggatat
    18121 taatgatgct tcagaatctt gataatacca aaacaaaagt tgacagtata agcataagaa
    18181 tgtaaaatca atacgtcaaa taatatatta attatataaa cctaatcttt ttaaggagtg
    18241 tatcctatgt tttcccttaa taatgtaaag aatgttcatt ttgtgggtat tggcggtatc
    18301 agtatgagcg gcctggctca gatattgctg gaatcaggct ataacgtaac cggctccgat
    18361 ataaacgaca gcaatttaat aaacaagctc cggaaaaacg gtgccgaggt tactataccc
    18421 cataatccga aaaatgttga aactgcggaa cttgtggttt atactgcagc agttaagcag
    18481 gacaattgtg aaataatcag ggcaaaggaa cttggaattc ctgttattga tagggctaca
    18541 ttccttggca tgataatgaa aaactataat tacggtattg cagttgcggg ctgccatgga
    18601 aaaacaacaa ccacttcaat gatttcaatt attattaaga acgcaggttt ggatcctaca
    18661 gcacttattg gcggtgaaat tgaagcaatt gatggaaacg tcagggtagg caagagtgaa
    18721 tacttcataa cagaagcatg tgaatactgt gaaagcttcc tgaaattcta tccctttgtg
    18781 gctgtcatat taaacataga aagggatcac ttggactatt ataaagattt agagcatatc
    18841 atatctgctt tcaggaagtt tgcaaagctg gtaccggcgg atggagccct tgttgtttgc
    18901 agcgataacg aaaatacact gaatgtctct aaagatgttg agtgtaaggt actgacctat
    18961 ggcataaaga acaaagctga tttttatgcc agaaaaataa aatatgacaa actgggacac
    19021 ccttctttcg aagtaatgca tagggataaa aagcttggca ccttctctct taatattccg
    19081 ggagagcata atgtgctgaa tgcccttgca gctatagcag cagcctataa tgcgggcgta
    19141 tccatgaata ttgtaaagaa aagccttaaa gagtttaaag gtacttacag gcgctttgac
    19201 ataaagggcg tccgaaataa tataacaata gttgatgatt atgcgcacca tcctactgaa
    19261 atcaaagcca cattgcgggc tgcaaagcaa ttcccccatc agaagatatg gtgtgtattc
    19321 caacctcata cctataccag gaccaggaca ctgttcaagg agttttctga agccttctat
    19381 gacgcagaca atgtgataat aacagatatt tatgcggcaa gagaaaagga tacgggactg
    19441 gttaatccct ctgagttggt ggacagtata aacaagtgca gtgcaaatgc tgtttataga
    19501 aatgatttcc gatgcatagc caatcttata gcccatgagg caaaacccgg cgacctgata
    19561 tttacaatgg gtgcaggtga tgtcttcaag ctggggctaa tgatacttga tgaaattaaa
    19621 atttaactga cagcttaaaa gtctttgcgg cttaaccgta aaggcttttt tatccgggca
    19681 gggaacattt cccttgtctc ttgttatact atattttcag tattataata agggagtaaa
    19741 acaagtccat aaatttattc ggaggtctta atggaagaga gtagtattaa cagtacatac
    19801 cataccatta atcattggaa taacttcaaa cttaagcttg tgttcgaagg ggtcatcgta
    19861 ggagcctttt caggtttggt cgtggtcctg tacagatatt tgattgaatt ggccggccat
    19921 ttttcaaaaa aaatatacct gatgcaaaag ggaaacaatc tgtttattgt tttgtggctt
    19981 gtatttctta tatttctcgg atattttata ggagtactca ttaagaagga gccaatgatc
    20041 agcggcagcg gaatacctca ggttgaaggt gtactcctca ggtatttcag tatgaattgg
    20101 tggaaaatcc taataggaaa gattgcaggc agtatattat caattggggc cggactctct
    20161 ctgggcaatg aaggaccgtc tattcaaata ggcgcagctg tcgcaaaggg atactgtgaa
    20221 acttttaaga agaaaaaact tgaagagaaa tttcttatta caagcggtgc aagtgcgggt
    20281 cttgcggcag cctttaacgc acctttatca ggagtaatgt tttccctgga ggagattcac
    20341 aaaaatttct ctcctgtggt cttaacttct gccatggcgg catcaattac cgctgacgtc
    20401 atttccaaat actttttcgg aatgaaaccc atatttaact ttcagggcat cagtcctttg
    20461 cctcttcaaa gctatcacta cttaatcatc cttggcatca tattaggttt gcttggagca
    20521 gtatttaatt attccatgct gaaagcaaag gatctttatt caatgcaaaa atggcttcct
    20581 gagcatttga ggcctgtttt cccatttata atcgccggcg tccttgggct ccttctgcct
    20641 gaagtattgg gaggaggcca tgagttgatt gcttcaatgg ttaacctaag ctattccttg
    20701 aatattctga tgctgatact tgtagtcaag tttatgttta caatgataag ctccggttcg
    20761 ggtgctcccg gaggagtatt ggttcccctg cttgttattg gtgcactaat aggcagcata
    20821 tacggtaatg tgctttcaga tgtattcagt tttaatcgtg agtgtgtaaa aaaccttata
    20881 atacttgcca tggcaggata tttcactgca attgtcagat ctcccattac cggaatcata
    20941 ttaattattg aaatgacagg atcttttaat catctgcttt ctgtgtcaat tgtatctgct
    21001 attgcttatg taacagcaga tatactcggt tcaaagccaa tatatgaatc actgctggac
    21061 aggatggtta ataaaggaag cagcagattc aaaggtgaca gcaagacaaa agtgataata
    21121 gaaacagcag tatgcatggg ttctctcatt gaaggcaaaa aaattaaaga gctgaaattg
    21181 ccgtcagagt gtttacttgt agcagttaag cgaggtgaac atgagataat ccccaaggga
    21241 gatacacaaa ttcatgcggg ggactacctt atcaccctga caaacgagga taatgcagcg
    21301 gaaatcagag aaaaactcac agcaatagcc gaagttcttt ctgcatcaat ttatgaaaaa
    21361 taagactaac ttattctgcg gaaagtgccc acaggtataa ggatgctacc gatccataag
    21421 gagaatatct ctttctataa ctttcaaact tctcctttga taattcctta atgccgtata
    21481 gattcatcat ccccctgcat atcgccagat ccttgaagct taccacatca gggcggcaga
    21541 gagaaaatat aagcagcatc tcggccgtcc atacacctac tccgcgcagc gaggaaagct
    21601 tttttattat ttcctcgtca gatagtgtat gaagggcagt gaagtccact tgcccggaca
    21661 aagcagcctc cgcagctcct ttaatgtatc cggccttcct ttcggacata ccgcaggctt
    21721 gtatttccaa aatttctgct tgtgcaatac tctcaggagt aattgttcca accagcgcct
    21781 ccagcctgtt ccatacagta tccgccgcct tgctggaaat ctgctggctg actatacttg
    21841 ataccagtgc tgtaaaagga tccggagtaa cctcacgttt tatcatgcct attctgtcaa
    21901 ttgccgctcc aagcttctta tccttttttt tcaaatattc tatttccttt tgcccatatt
    21961 caaatacatg cattatttat ctttctccaa ttccaacaat ttctttttca tgtccaatcc
    22021 tcccgcataa cctgtaagtg aaccgtctgc acctatgacc ctatggcacg gaataaatat
    22081 aggaatagga ttgcgattat tggcaaggcc cactgccctt gcagccttcg gcctgcctac
    22141 cttcccagct atctccccgt atgtggcggt cttgccatag ggtattccgc acagagcagt
    22201 ccaaacctgc ttcataaagt ctgttccgga gggttcaaga ggtaaggaga attctttcag
    22261 ttccccggca aaataactct tgagctgcct tgctgcttct ttcagcaatg gtgtttcaca
    22321 taattgcata tcctgggaca attccttgtc ggtaatgtaa acattagtta ttttgctgtc
    22381 cttttcggtt attccgattc tccctatcaa ataatcataa aaatatgtat acactataaa
    22441 acacctctgc tttttctgta ttcattcgga gtttgtccaa cctgctcttt aaagtgctta
    22501 tagaaattgg ataggctgtt aaagcctgtc atgtaagcaa tctcgataat atttttatct
    22561 gcctgtccaa gaagttcggc tgatttgctt attctcaatt tagtagtata atgtttagga
    22621 gtaagcccgc tattttgcct aaaaagtctt gtcaagtgat ttgtgctgac tccaagctgc
    22681 tttgaaatat ggcttatatc tatcggcttg tcataattcg cttcgattat atccttggct
    22741 ttattcacca gttccaagtt cggttcaaaa actgcttcat caggccggca ctttttacat
    22801 gggcgaaatc cttcatcaat tgcatttact gcgttatcaa aaaaaatcac attcccgcgt
    22861 acaggggtct ttgccctgca agaggggcgg cagaaaatcc ctgttgtctt tactccgtat
    22921 aaaaacaggc cgtcatagct tttatcacag tttacaacgg cttgccattt ctcattatcg
    22981 gatattcgga agtgttcttt catggatttt ctcctttcgt tttgaaccga taagcatatt
    23041 ttacatgagc cataaatata aatcttcctc aatttcacca atcaatcttt tttagcttta
    23101 gttccataaa acgtgtgcct tggatgggat tatacacata gggctcaata tcatagaagc
    23161 caaaggataa atataaactc tgtgcgctct tcatggtcgg aagggtgtcc agcctcatat
    23221 attgatagtt attattcatg gcttcatcaa taatcattcc gacaagcttt ttacccaagc
    23281 ctaatccgcg gtattcatcg cgtacgtaca gccttttcat ttcgcagata tccttggaaa
    23341 tcttccgaag tgcaatacac cctgcctccc tgccctcgac cagcgccaat atcagagccc
    23401 cgtcaggagg ctgatactta cccggcagtg tattaagctc tgtttcgaaa tcctgaaaat
    23461 caagatcaat ttccaatgaa cgcgcgtatt ccaaaaatag ctttcttatc tcttcaatca
    23521 tttctccttc tgttacaaga aagaattccg gtttgttcac acttatcact ccaaaacact
    23581 ctattcattg ttaagttcat acatccggca tatatgtatt tgatgccgtt aaatctgaat
    23641 tgacaataaa tcctcaactc tgtcattcat atatttttgt gaatcgcaga ctcctttatt
    23701 gtaaaactcc ggtgccaatt cttctataat aaagtccagt atcaggcttg cagccaaatc
    23761 gcccagttct tcatcccttt ctttcagaaa ataatacttt attgcagaaa gcatatcatc
    23821 tcttttttct ttggatactt taattctatc cctattcttc atgtaatgcc tccttgattc
    23881 ttagttatat ttatatttta tcagtacata tgccttccta caattatgaa aattgcaaaa
    23941 aataaaatac ggcagatgca gcgcctgccg tattttactt ccttatgcat ttatcctaag
    24001 cttttttatt tctggtcttt acgtcaaaaa ttactgccgc taccaatacc gcgcctctga
    24061 ttatgtactg ataggaaata tcaacaccca tcagattcat gccattggtc agggatgcca
    24121 taacaaaggc accgataatt gagcctgtga ccttgcctgc accgccggct gccgatgctc
    24181 cccccacata tgcagctgca attgcatcca gctcaaaggc gagccccgct gtagttgttg
    24241 cagactgaag gcgggcagta tataatatcc cggacaaagc tgccagcata ctcatagaac
    24301 tgaatacaat catagatact ctgtttacat ttataccact cagctttgcg gcatcgggat
    24361 ttccccctat tgcatatata tgtctgccca ataccgtttt ttcggtaata aaattatata
    24421 ccagcacaac tattatgact actactactg tccaggaaaa cccgttataa cctgcaagta
    24481 tccatgttat gtatccgatt accagggata caaacaacag tttgacggta aacatcacat
    24541 tggataaaac ttcaaagcca tatttcagtt tattattgcg attgttgaat tccgccaaaa
    24601 tataaagtac aatcgctgcc gcacccaaaa caagtgttaa tccgtgaaca ccgtttatgc
    24661 tgaataattt cggtatatac ccgtttccga tagcattaaa atcggcattt ggaactatta
    24721 tcgttcccgt ggatgaggta accctgagca aagcccccct gaatacctgc atacctgcca
    24781 aagtcacaac aaaggaaggt atactcattt tagtaaccaa aaagccgatt atgattccta
    24841 ttgatgcccc caaaaccaat acaactgcaa ttgtgatcca gacaggcatt gcatattttg
    24901 tcaataatat tgcggcaata gctcccagaa aaccggcaat atatcctacg gataaatcta
    24961 tatgcctgat tactattata agggtcatgc ctacggccag aaccgcaata tacccggtct
    25021 gattaatcag gttgctgata ttccttgagg acataaaaag accgtctgtt gccattgaaa
    25081 aagtcagcat gatgacaacc agagctatat acatcccata ttcacgtata ttcttcttta
    25141 ttaaatctct cgcttcatta acaattccca taaccaagcc tcctagttag ttgccatttc
    25201 cataattttt tcttgagtcg ctccttctgc gggcaattca cctgtgattc tcccgtcaga
    25261 tacaacatat atcctgtcac tcatacccat aatctccggc aattcagatg aaatcattat
    25321 tatactcata ccgtcttcta ccaatcggtt cattaaggta tatatctcat actttgcgcc
    25381 tacatcgatt cctcttgtag gctcgtccag aatcagtacc tttggcttaa cgtacagcca
    25441 cttcccgaga gaaaccttct gctggttgcc gccgctcagg ttctcaagtt tttgttctat
    25501 actgggagct tttatatcta atgaaccctt gtaataattg gcggttttta cttcctcatt
    25561 ttcatttaca atgccttgct ttgccagggc tttcagattg gcaatggtaa tgtttgtctt
    25621 aatgtcgctt atcagtatca gtccgttgcc ctttctgtct tccgtaacat atgcgacacc
    25681 ggcttttatt gcttcatggg ggcctctgaa gtgtttcttt tgccccataa catacaagtc
    25741 accgctgatt ttatattcgt ccggatttcc aaaaatgctg tgggcaagct cggtacgtcc
    25801 cgctcccatt aggccggcta taccgacaat ttcacctttt ttcacaaaaa aactcatatc
    25861 cttaaggatt tctctccctg tctttgaatc acaggcattc cagttattaa cttccagagt
    25921 cttctcctca tatttttgca cgtttctttt aggatagata ttttcaattt cacggcctac
    25981 catatgcttt attatatttc cttcaagaat ccctccatcc cgggctgaca ttgaagctac
    26041 ggtccgcccg tcccgaagca ccgtaacatt atctgctatt tcaataactt ccttgatttt
    26101 atgcgatatc attattgaag ttactccgtg ctccttaagc tctcttaaaa gctccagcag
    26161 gttttcgctg tcatcctcat taagtgcggc agtaggttca tccaatatca gcagctttac
    26221 attcttgctc aaggcttttg ctatctcaac caactgcctc ttgccaaccc ccaggtcctt
    26281 aaccttggtt gcaggattca gattgttcag cctgaccttc tttaaggcct cccctgcctg
    26341 caagatggtt tcgttccagt caatcaatct tccttttttt atttcatggc ctaaatatat
    26401 gttctcgtat acggtcattt cgggtactaa agccaattct tgatatataa ttgcaatccc
    26461 ggctttttca ctgtcactga tccctgcaaa ttttctgaca ctgccgtcta gtataatgtc
    26521 tccggagtat tctccaaagg ggtaaacgcc gcttagaacc ttcattaagg ttgatttccc
    26581 cgctccgttt tctcctacca ggcagtggat ttcgccattt tccacctgaa aatttacatt
    26641 gtccagagcc tttactccag gaaagacctt tgtgatatta ttcattttca atatatattc
    26701 cttcatagcc atatgctctc ctcgataaat caatcacagg gttacaaaag gtatgccaat
    26761 atatagctga atagtgatag acagagctat cactattcag ctatttttat ttatgcaaat
    26821 aattacttca ggttggtaaa atctgatgca ggatagtatc ctgagtcaat caatgctgat
    26881 ttaacattat cagctttgac aacgataact tcggtttgct ttgcttttac gtctattttt
    26941 ccgttattgt atgagcctgt cgtttgcggc tcttttcctt ccaataatga aactgccatt
    27001 gatattgagt ctttaacaag ggtacggaca tctttgaaaa cagtcattga ctgtttaccg
    27061 tcaataatgt attgaacaga agctttttca gcatcctggc ctgttacctt atagctggtt
    27121 actgccgggt cggcggcaaa tgcatcagct attgcacggg cagtaccatc atttggcgct
    27181 aatatgaaca cattgccctt atcagctgcc tgtgctgctg tcaggtgtgc ttcagctttg
    27241 ttcttggctt cattgaaatc ccagtttgta gttacctggc caattatctt acccatttcg
    27301 tctctggtga gcttggcttt cccctgaagg gcaactgctt cgcttgagtt ctttattaca
    27361 aatgttccgt cggctatttt gggctgcaat acattccagg ccccttcaaa gaataagaaa
    27421 gcattattat ctgaagctgc gcctgcatac aagtataacg gattaccgct gcctgatgcc
    27481 tgatcgacca ggtattttgc ctgagcttct ccgacagcta tgctgtcaaa ggtaacataa
    27541 taatcaaccg catctgttcc ggttatcagc ctgtcataag atataacctt gatgccttct
    27601 tttctggccg cttctactgc agatgctgca gcatcgccat catgaggaca aattattaga
    27661 acatttattc cttttgcaat caaagcatca acattctctt tttcctttgc cgaagaaccc
    27721 tggctgaaca gaatttccac tgaataatca gtacttccca aggcttcgct aaaccttgtt
    27781 tcgtcttgaa tccatcttgg ttcatccttt gtcggaagca ctataccgac atctaccgtt
    27841 ttggaatctc ccccggtctc accggatgca ggagccgagc cgcacgccgt caatgcaaac
    27901 aacattgcaa atgccattaa tactgcaata agccttttca ttttttcgtc ccccttaata
    27961 atatttttga tatcaagttt tgaaagctgc attctgcagc ttccggttca attatttcat
    28021 gaattataca tcatgtctct gtatgcattt gccggattac attactattt ggcgcaattt
    28081 aaaaacaccc caatatgaca gtaatatttt gctgtaaaat aataccgcct gcaagctaaa
    28141 tcctgcaggc ggcatattta atgcctataa acctcatctt caaggtgaaa tccatctctg
    28201 attatcgtct catctatatt ttctttatca actgctattg gggtcaggac ataatatttt
    28261 acctcatagg taccgtcatt tatatagtcc tgtacattca gcggctcctt ctttacaagt
    28321 ctgactgcca tttcaaccgc tgcctcggcc agcttttcta ttggcttgta tacggtcatc
    28381 agctgcatac cttcaactat cctctgacat gccgtcaagt ccgcatcctg tcctgtaacg
    28441 gatacctttc cggccagcct atgctcggaa agcgcttcta ttaccccctc tgcaaggctg
    28501 tcatttcccg aaattacggc atctattctt atccctttct gcagcatatc gtctacaacc
    28561 ttgaatgcgt attccttcag ccaatctgca gcccattcct ctgcaataat cttaattttt
    28621 ccctccgctt ccaacggccc caagatctta tcataaccct gttttatcat ctttgtattg
    28681 ttatcggttt tggcgccgtt tactatcagc aggttttccc ctttcatgct gtctgtaatc
    28741 ctctctgcca tcagctctcc aaccttgaca ttgtcaaatg tgacatacaa atccacattt
    28801 gagttcaata ccagcctgtc ataggatata acaggtatcc cctgcctttt ggccagttcc
    28861 actgcatggg cagctttatt caggtcgttg ggaacaatta tcagtacatc tatgttttgc
    28921 tcaagcaaat atctaacctg cttcatctgg tcctggtcat cattgtttgc attttgtaca
    28981 ataatatccg ctccaaattc ccgtgctttt gctatcaaaa tatccctatc ctttatccac
    29041 ctctcttcct ttaaagttcc gagagaaaag ccgattttta tcttttctct ttcttcaata
    29101 tttttattgc tttgaaaaaa gcctctgtct gttctgttca atatactggc aattgagagt
    29161 ataagcataa ggaatacagt aaaaagcacc acaatgattg agttttttac tttattttcc
    29221 ttagccaata tcatcacctc ttatgtttat tactgctcta tattctgtgg gtgtcattcc
    29281 cgtgatcttc ttgaatattt tgcagtagta attgggatcg ttatacccta tcttgaagca
    29341 cacttctttt atgctgattt tgtgatcctt caggaatccc attgattttt ctattctcaa
    29401 ctcggtgaga tagtcggaaa agttcttgcc cgcttcatcc ttgaatattt tgctgaaata
    29461 atgataactg agatttacat atcttgctgc atcggcaagg tttatttcct tgtggtaatt
    29521 attatttaga tattccagca ccttcggaat tattccatcc ctttcgcttc ttatttgctc
    29581 ctgtatctcc tccgacagct gggagatgat atccataaac tgtcttttca atacttcctt
    29641 gctgttggtt tttaatatgc tgaaaatata agattgtttt gatttgctga aggagcttgc
    29701 cttatacggc agggcttttt ctatgacaaa tagcaggtca ataagcttgg actttatttt
    29761 atccatgtcc tcataatagt tggtgattaa ccaaagatat atttcttcaa aggcttcccc
    29821 ggcaccttgc aggttccccg taagcatcct gtttgcaaat attgcctcct tttgtgcggg
    29881 ataggtatcc ggtatattgt tggatggcgt aatgtcctcg taatgcataa cagcatgtcc
    29941 atttgataca gaggcagcta tataagcctc attgcacgac ttgttaaagc ttcctgcctc
    30001 atagcttctt cctataccta ttgtgtattc gatacccgta ccgggtttga ttctgtcaat
    30061 aatccgattt gcaattttta tagattcgtt tctgatttca taagagtcaa tccccttttc
    30121 cgaaggtata aatgccgtga ttctatccaa taacggattt cctataagac aagggcatat
    30181 ccgctttaac tcaagagaaa atttctcata gaaattctgt ttctccaagc ttaatttcaa
    30241 gttgtcttct tttatcttcc cctcaaaatt tttcaaaaga actgttaatg cataaccctg
    30301 cctcagattt atattgaaaa tttcctcata aaattcaata tcgcttattg ttcctgaagt
    30361 aaacagctga taggatacaa actgactttc aataaagggt ataatcctgt tgatcctttc
    30421 cttcaattca gcttccttca gcaaaagcct ccggttttgt ttgatggtat tgcataagtt
    30481 gtttagtgta tcaatcactt tgctcttttc tataggcttc agaatatatt cagatactcc
    30541 cagattaatt gcctccttgg cataatcaaa gaattcataa gccgtaataa ttacaaacag
    30601 aatactgctg ttaacggctt ttatctgtct gattgcctca atgccgtcaa taccgggcat
    30661 gtgaatatcc ataaatataa tatccggctt cagctcaata gccttttcaa tggcttccct
    30721 tcccgttgaa gctgttgcca cgatatctat gtccttcata ttcttccgga taatcatttt
    30781 taaggagtcc agaacaaggt attcatcatc agctatcaga agctttgtca tatgtatcct
    30841 caccctccct tcttgcacct ttcattatcg gaagcttcaa ggtaaccttt gtgccgtggc
    30901 ctatttcact ctcaatcgat aacagcttcc ttggatcttc tatgttaaaa aataattgca
    30961 gcctgtatag cacattgctc attccaatgc ccgtaagacg attcctgtac ttactgccat
    31021 cctcttcaaa ccgaagtatt tcttcaatgc aggcagaatc cattccaatt ccgttatcaa
    31081 taacctctac cattatgcag tccccctccc gccttacatt taaatgaatt tctccatgcc
    31141 tctcaaggtt ctggaggcca tggataaaag cattctcaac aataggctgg attactattc
    31201 tgggtatcat aatgtcaaag gtcgtttcat caatatcggt gtaaaagtct attctgctgc
    31261 cgaaccttgt cttaagaatt tgcatatagt atttcacata ctgaatttca tcctccaggg
    31321 ttgtcagctc atctatgttc tttaaattgt acctgaataa gttggatatg cttacaatga
    31381 attcggagga cttgtccgcg ccttctatgg cagccaattg agcagctgca ttcagagtgt
    31441 tgaaaagaaa atggggatta atctgggatt gaagaaattt caattccgcc tccttaagat
    31501 gactcatcat cttcaggttc tgcatttcct gctccttaag acgcttctcc acctctgcct
    31561 gtctctttaa ttcattgatg tatgctttga tactgtttac catttttata aaggctcccg
    31621 ccagtatatc cgtttcatcc ccggtatgag tctgcctcag ctcaatgtca aaatcccctt
    31681 cggcaatttt ttctgcggaa taggacagct ctgaaatagg cttggtcaac cgataggttg
    31741 acacaaccgc aatataaatg tttgccagca gcgcggcaat aattatcaat aagttcatat
    31801 aacttataaa attcatggtc ttattgatat tttcatattt ctccgttcct gattttaatt
    31861 tactgtccag aatcttatta ttataaagct taatatagtc gcttatacga tttgatcttt
    31921 gaaaatgtga aatatatctg ctgctgatcc ttccgcgctt cgcctgtatg gcattgtccg
    31981 tctctataag cagctcatct atcatattcc cgatatcctt catgagcagc aattcattat
    32041 tatactctgc agttctgggt atctttcttg tggtttcctg caatatattg taatgagtat
    32101 agtaattaag caggctttct gaggatgctg tcatcagata cttttccagc tcagtcataa
    32161 gcgagtttac atcgttgttc aggctgttca ggtaggaata atcagtaata atgctgtttg
    32221 tattcctcaa aaccaccctt gcattgtaat acgaaaaaat gcttataaca cccagaagca
    32281 atatggtcag cagaaaatac attatcaatt tttttcttat accaataaag gttaataatc
    32341 cagggttcat attctcaccg tctattccaa ttctccgcag cccttttaat ccagtttgta
    32401 tgcctttttg tagctgctca gatttttctt tgcgataacc ttaacctgtg catcaataaa
    32461 actggggaca cttttgccat tctttacgtc tacaagtgcc ctgacgcttt catatcccat
    32521 tttgtacggg tcgctggcaa cagttccgta gattatttct ttatcaatgt acctcagtat
    32581 ttcttctgaa ttgccatatc ccaccaatac tattttacct actttgcttc tgtcaacaat
    32641 caactgggcc gagcccaagg tatcggctga accggcggta aaaattgcat tgattccctc
    32701 atcgctgtca ataatgctct gagttatctc ttccgcgctc aatatgccca tctttgaggt
    32761 ataggtcctt attattttca tattgggata ttctttcagc gtgctgataa aaccgttcat
    32821 tttcaaatta tactctatag cttcctgctc caaatcaccg tttaagatta ctgcaacctt
    32881 cgcatcggca ttggtggctt cgattaataa tctggccgct tccctcccca attcaaagct
    32941 gtttgtaccc gcaaaggcat atcttttgct ctcactgtca tcattttcta cagttatgac
    33001 aggaattcca ttgtcgtaag ctttatcaat catttctgta aagcccgtgc cccctacatg
    33061 ggtaattatt ccgtcaacct ttgatataat cgcaatatcc ataaactgca gttcctcttc
    33121 aggattactg aaacgaggtg cgttgtattc gactactaca ttattatctt cagctgaatt
    33181 ctgaactcct tgcattactt cattccaaaa gggatcgaca gaattctgcg caataaagta
    33241 gaaatgatat tccggcttgg aaatataggt ctttatatcc tccctctgca gccagaacag
    33301 cacagcactt gcagctatta ccatcagagt cagtatcaaa atagcatgta catataagaa
    33361 tcgcagcaga tttctaagta tcttcattag catatttccc cttaaatgga attatgtatc
    33421 ttatattata acacagaaaa aatagttttt gctttgcgtg aaaaataaaa agggccaggc
    33481 ttgccatgca gctgtcctgc ccttatccta tcattatgcg attgaatcca tgaagataac
    33541 agtcagttct tatctatttg aattatgaaa aaacaatacc gggtttcacc atttttctgt
    33601 aattcagcca cataaccggc tgtaatcttc tcattttctt cgtaatgtga actaaggcct
    33661 gcttgaagca gcctctctgc agtaaattca tattttgagt ccccgatctt ttctacttca
    33721 acatccatat ctaacggtgc cccgaaagaa tcgttataat taggggctcc ctccctgtta
    33781 atccagtatg ccttaacgct tgcttttaca tcttgctcct tcatatccag ggttattttc
    33841 gttcctctgg ggacatatat cagcttttcc ctgtcaattt ccaggtaagc tttcttaaaa
    33901 tcctcctttg atgtaaaggg ccttgaagta agcgtatagt tgatatcctg ttgaccaact
    33961 cttatatgta tttcattttg agccgctttg tctgaggctt tatcgttact gttatctgtg
    34021 acgcaaccca ataatggtac gacaataagc aaaaccaatg caaaacttat aacccttttc
    34081 atttaaaact ccttcccgca ggccttgcag aataaacaaa tatgttgata tccacttccg
    34141 tggcaaattc actcagcttc tccagggcgc ttgtttctgc atgccggctg taaggtgtca
    34201 tcgtaaaaag attcagaata tcctgcttgc tttttaatat gatttcgtag tttacctttt
    34261 tcttttccaa aagggtaaac ccttctccca cagttccctt ttgaccgatt tcctccggac
    34321 tttcatacag tgcttccctg atactgtata aatgccgtgc cccgggagat gcaactataa
    34381 gctttccctt tggtttgagt attcgcctga actcgccttc atccctgggt gcaaagctgc
    34441 acagaatgca atccagtgta cctttcaaaa ccggaatgtg ataattgctt gcaactgcaa
    34501 atctgatgcc cttgtccctg ccggaggcat aatgtattgc agcctttgga acatcaatgc
    34561 cgtaaatatc agtttttata cagtcacaga aatctgtcaa gctgtttttc agcctgcaga
    34621 tataataccc ttccccgcat ccggcatcca gaatgctgac ggtttcttcc gaatcttaat
    34681 caattaacag cgcggataga acaatgacat tcagcatatt ggaaaatccc tcataatacc
    34741 ccttatcaag gaactccctg cggctctgaa gcatctcttt gctgtctccc ggagtgcctg
    34801 ccccggtatg gctgggaagc agcaggttta catacccttt ttttgcaatt tcaaagctgt
    34861 gcccggtggg gcaaaaatat tgtctttccc cttgctttaa tgctttaaag caatttgggc
    34921 attgaaatat ggaatgcatc attatccttg ccatagatgc cgctccatat cgatagttcc
    34981 gtctgccctg aaggtaatgc cctcagcttc aagcattgcc ctttgtattt cggggtctcc
    35041 gaacacatgg cctggggata gctcccccgt tgctttaacc accctgtggc agggaagttt
    35101 catgtcttca ggagcacttc tcatagccca ccccactgtc ctggcacctc cggagcatcc
    35161 cagcatcctg gctattcctc catatgcagc aaccttaccc tccggtattc ggccaactat
    35221 ttcataaact cttttaaaaa aaccttctct cttgtcagac attacctttt ctcctcttac
    35281 accgttatct ccaatctatt tccgtcagga tccaatatgc agctttcata atagccgtct
    35341 ccagtatgac gaggctcgct gactaccgta taacccgctt tccttaattc atccgtaaga
    35401 ctgttaacct tttcggcact tcctgttgaa acagcaatat gagttaaccc gatatgctga
    35461 ttgattgtat catttgaatc caatggagac ccggacatct gcataagttc aagtcttgca
    35521 cctgaatcaa atttaataaa atacgattcg aaagacttct tgggatttat atatttctct
    35581 ccggcaatcc catcaaaata atttgtataa aacattttta atctttcaat atctttggtc
    35641 catatagcta tatgctcgat tttcatgatt aatataatcc ccctccttaa attcttacga
    35701 aaagctatat aaaagacata ttagaagaat aatgcgacgg ggacacgggg acgtttcgcg
    35761 tgtcccactg aaagcgggac aggggaaacg tccccatgtc cccgggagaa acgtccccgt
    35821 gtcctcgtgt cgcattcttc atattatgcg atgcaattag ttatgccttg gttagacttt
    35881 atctgattat ttcatacttt tctgcagaca gtgcatttat cgccgtatca atatttttat
    35941 ccttgacaag aatataatct gtatcgtatg tagaaattgc aaatatgctt atccccttat
    36001 ttgcaagtgt tgtactgatt gaggaaagga ttccgatcaa tgaaaaatcc aatggacctt
    36061 caacccttaa tattctccaa tccttttcac actttatttt atccggaata ttatcttgag
    36121 aacatacaac tgataattca tccagcgttt ttgtaacgga ataaaagctg cattgggata
    36181 cccattcggg aattgcctcg tccttgtcta acctgcagac tccatacaat ccctccatta
    36241 acttcattgt taaagctttt tctgtcatat ctcctcttcc tcctctgata tcttagtcat
    36301 attattgtct cttttcgtac ataatcaaca attttttacc attgcccgac gcatattatc
    36361 ctttcacata ctgcaactaa caacgttgta acgatattgg acaatagaat tgaaacaatc
    36421 agattctttc tgttcaaaga atctttcctg agcagaaaat acacaattgg caattctacg
    36481 gcaacagtca atattaagta tcctgccagt acaatatagt aggggccttt attgaaagct
    36541 gcaaatatgg aatagccgcc gcttgttgga ataaatctgt atgccctctc caaatatggt
    36601 gcaataaaag acattaggtt tgcaaggcat ataatataaa atgctttcct ggaatctgaa
    36661 actccgccta attttgttac gaaagctgtt tcaataagta ttgtaaaaac aactgcaaag
    36721 ggcaagacct tcattgggct tgcagtcacc caatgccagg atgaatttgc atagacaatc
    36781 gacgaaagta gaaataagaa ggcaccagcc catacggctg ctttataggc tttatgtttc
    36841 atatataccc caccctcact caaatttctt cataatacat aaatattatg caatatcgaa
    36901 cttataccgt taactccaat ctgtttccat cagggtccaa tatacagttt ccatagcaac
    36961 cgtctactgt ataacgaggc tcactgacta ccgtataatc tgcactcatt aattcatacg
    37021 taaggctgtt aaccttctca atatctccta atgaaacagc aatgtgtgtt agtccgttat
    37081 accggcagga ctgtaattat atatcctccc gcaaacaagc tcaggaaatt cgctgctatt
    37141 acatataata ctgttctcct gcgactatgc tcttttatca atgtaaggaa taaaacaatt
    37201 tctgcaatga atacaaagaa ttcagcaaat atcagtgaaa aaaccagata gcttgccaaa
    37261 gggacagagc tattcagcca aatatttaat gctccctgag tgatcagatt tattattaga
    37321 aaagcaagcc atgagttctt tttcctgaaa acgaacaacc agaatacaat tgcttctatt
    37381 atcagtgtca ggataacacg cagagacact aagagaattg accttgacaa cgatttgccg
    37441 ggtgtaagtg tttgattctc caaatccaat gtatagatgt tgttgtatgt tttcagaggt
    37501 tcttccagta atacttcaaa actgtcctct cccgtactga tattgatatt gtaatccttt
    37561 gctatcctta aatctgccga gtaaaaaaca taataccttt caataatttt atccctcatg
    37621 cttgctttaa tatctgccgg gccaatgctt atctccagtt catcaggcgc gtcaggggct
    37681 ataatcagta ttgacggcgg ctctgcagaa tttccatagc acacagcaga gacgatgatt
    37741 aacattgcca ttgacaaaat cattatcagg agcagtttgt cttttttaca catctatgac
    37801 agcctccttt taaagcattg aaagcattgg ggacggttct ttttgcttcc gaaactcagc
    37861 ttcttttctt taaatccttt ccgaaatgca ctatacttgc acccaatgcg gataataccg
    37921 tataaacaaa tacccataca agaaaagaac tattgaatac tgtaaatgca gcaatagacc
    37981 acaatgcaaa agttattccc gcaactatga atatttttct tatcaatact tgcccgataa
    38041 taccaaacaa aaaggataat aagggaaaga taagcaatgc catcagaaat tccatagtta
    38101 acccctccta taaacaaaat gtcattaata agaatgatgc gtattaacat gtacgggtag
    38161 cttagaatga cttattaatt attcttcctt caataatttt aatttcttta ataaatagct
    38221 ccatgctgat tatttgctct ttagtcagac cttttgcatc aaagtggtta tccaaatttc
    38281 aataaatagc tccatctaag ccgttaatga tttcgatacc ggaaaccaat ccgtcttcaa
    38341 tgagaaaaac cacgcttttg gtgccttctt caggggtatg ggcataggca aggtcacatt
    38401 tgccgaacca tacctcgtcg tcgtaggaga tgctgtccat cttctttagc ggtttttcgg
    38461 tccaatgttc taaaagggtt tcctctgtgt cgcctatggt gacgcccgct gaggaacagc
    38521 cttccttgac agtacggata gtgataatgg tatgcacgcc ttcatcagga ttaagatacg
    38581 ttacctgcag attatcgctt tccacaacag ttacgttacc aaagctctca gccctgaagc
    38641 tcttgcggct cttctcggtc agatccaagc tgaaagggaa atccaccgga tacgcattca
    38701 gagatagggt gccggttccg gggatagaaa gagaaaatat gcttccgtct gagcgttggt
    38761 ctaagcccac atccgccttg ttgccgtctt caattggttt ttcacaatac acatttgata
    38821 caaagagata gctgccgtct ttttcctgca gtgtcagtat ctttttcccg tcggaaaaaa
    38881 agacgtcatc gtagaaaaga cggacgatat ccccatcccg ctcgcctccg gaaaaaataa
    38941 tctccattct ataattggtg tcaccgtggt aatagtaata ggcatcatat tcctccaggt
    39001 aagtaaaatt ctccagccct atttcatctg tatccgctaa agtcagtccc atatatttgc
    39061 caagcaccga ctccatgttg gagcggctga ttttcataca ggcgcaatcc ggctccatat
    39121 cccagttgtt atatgcaaca acagctgctc tttcctcttc tgtggaaatt tcttcaatgc
    39181 cgctgccgca atagaataat tgaaatatat cgattttttc gggtctttca tatgtggagc
    39241 tcaaaaactg attgcggata ttcaagtatt ctccgttaaa gaattcgtct ccgttgaagt
    39301 atgaaagctc ctcatcggtc aaagctgtta tctccttatg acttttcccg gtatcattac
    39361 atccggatac aaaggccata atgagaaaga tggcaagtat tgtgctgagc agcacattag
    39421 gtttttttac aatcatcata atctactcct caatttactc ggaaactttt tgatatagct
    39481 aaaattacct gctgttccat tcctctaata atagacgaat aaagtttcca aatgtttcat
    39541 atatcaagaa tgatgcgacg gggacatggg gacgcgggga catggggacg cggggacatg
    39601 gggacgtttc ccctgtcccg ctttcagtgg gacacgcgaa acgtccccgt gtccccgccc
    39661 gtgtccccgc gtccccatag catttttatt ctcatatgca atttcctctt tccattataa
    39721 cgcaaatcca aatataattt aacaataatc ggataatata tgcgaacata tgtttgcttt
    39781 ttgcggtaaa gtttggtaat ataataatat aaatatcttc aggggtgttt ttatgaacct
    39841 taatgagaaa atcaaaatat tatcggattc tgcgaaatat gatgtatcct gctcctccag
    39901 cggaagcagc agaaagaaca aaaaaggcgg tataggaaat gctgctcctt caggcatctg
    39961 ccacagctgg actcctgacg gcaggtgtat atcactgttg aaaatactgc ttacaaacta
    40021 ctgcatatac gattgcaaat attgtgtcaa ccgctcttcc aacgatgttc ccagagctgc
    40081 ctttaccatc gatgaactgg ttaaccttac aataaatttt tataggagga attatattga
    40141 aggccttttc ctaagctccg ccgtatgcgt aagccccgac cataccatgg agctgctcct
    40201 gaaatctgtc aaaaggcttc gggaggaggt caatttcaac ggatacattc atgtaaaggc
    40261 cattccggga gcaagcaata agctgataga ggagacagga aaatatgtgg acaggatgag
    40321 tgtcaacatt gagcttcctt cgggcgaaag cctcaagctg ctggcgcccc agaagaccaa
    40381 ggaatcaata atcaagccta tggggctcat aaagtcagga ataattcaat atcaggaaga
    40441 aaagcttaag tttaaaagca ctccccaatt tgtcccggga ggccaatcca cccaattaat
    40501 cgttggtgcc acaaaggata atgatttaaa gattttgaag ctgtccgagt ctctttatag
    40561 cagctacaag ctaaaaaggg tatactattc cgcctatgtg ccggtatcaa atcaccctaa
    40621 tcttccggca atcagcggtc cgcccctatt gagggagcac cggctgtatc aggcagactg
    40681 gctcctccgg ttttatggtt ttaactccgg tgagttatta aatgaagaaa acccgaactt
    40741 tgatgagctg ttggatccaa aaagcggctg ggctatcagg aaccttgaca aatttcccgt
    40801 agaagtcaac aaagcagatt attatacatt gctgagagta cccggaatag gagtcaaatc
    40861 agctcaacgg atagttcagg ggagaagggt atccatgctt gactttgatg acctaaaaaa
    40921 actcggaata gttctaaaac gggcaaaata cttcattacc tgcaaaggca ggtatttccc
    40981 gcaaaaaagc ataccgacca atgctgtatc cataaaaaac aggctgctgc tggaggataa
    41041 tattaagaac cgcggaaata ttgagcagct ctccatattt tcacttttcc cgggcataat
    41101 ggatggtgaa ttttgatgct gtattatatc tatgacggca gctttgacgg gcttttgaca
    41161 gccatatatg atgcatacta caataaagat tttcctgagc aaatagtacc ccaggacagc
    41221 ttcacagaca actttttgat taacaaggtt tacatccgga cggaagaaga gaaatcaagg
    41281 aaagtccatg atgccgtact tcaaaaaata tcaagacggg caatggctgg tattatgtat
    41341 gcttatttat cggaagatga gaatgccgga atatctattt tggaatacct gcgttttggc
    41401 ttcaaagtcg gaagaactgt agattcttat ctcgccgatg acagggtaaa caatattaac
    41461 agactggtaa caaaggttgc gcgggaaaag catctgctgg caggaattat aaggtttcgt
    41521 ctgcttcata acaacattta ttatgctccc attgagcctc agtataatac attatgcctc
    41581 ctgggtaaac attttgccga caggatgtct gatcagatat ttgtcataca tgatgtgaaa
    41641 agaagctatg gagtttttta cgacaagacg aaatggttca tgagtgatat tgaaatgaat
    41701 aaggacattg tattgcacga aaatgaacta ttctaccagg agctttggaa acgatatttt
    41761 gataatataa ccataagaag caggataaat ccaaagctcc agtcaaatca catgcctaaa
    41821 aagtattgga agtatttaat tgaaatgaaa taatcatctt gtagtcaatg tgctgttgtt
    41881 tttgcccata atcacttttg tatttacttt accgatcctg actttttcga tccatgccga
    41941 cagagtatcc agcgccgctt caaggtcttt gtgccttaag gggtccaccg actcaataac
    42001 agcgattgca tttttcgtat cttcggtaag catcgttctc tgtccgtccc tccagttcca
    42061 gcagcggcat accgctcctt catcgtccag atagcagatt tctccgggga gagtgtaatc
    42121 aatttcttca tcgcctaaag caagaaacgg gtcacctcca tttgtaactg taagtctaag
    42181 atctcctttg aatgtatcaa catcctccat tccgcacggt aaggcgaatc ggagcgaaat
    42241 tccattatat atgtctacca gcgggttaat cactcccact tcatttcctt tttcgatgcg
    42301 tttaagcagg gcttcaatgg aggaccttgc cccttttttt gtcttaaatt ttgaaaatgc
    42361 atccctccaa acggatatta ctctgttttg gctcagtaca ggttccgtta aatacttcct
    42421 cgcttcctgg tttgattgtt caaggaattc aataatatca gaccggagct tttcgcatcc
    42481 ctcctcggtg ttgtcaatat cattaatcaa cagtacacca atctcacatt cagggaagat
    42541 atcccagaaa ggctgttcaa ttataaatct gctcattttc ttcctccgtt cttttttcgt
    42601 catattttaa gaataatgcg tcgcaggtaa cgggtaacac taaacacacg gatgtatcac
    42661 ggattcctgc ggaataacac ggtaactatt atatattata tagctgcatc aggaaacttt
    42721 gatacttaca tcattatcga taaatattta tgtgccgaaa tatttatccc gccgtgcatt
    42781 tagtgatttt tcgtgtcttc agtgttacca aggcttcaaa gccctaccct gaatctcgta
    42841 tctggtgtct cgcgtctagt gtctgtgcac gttagtgcgc atcattctct gctttttttc
    42901 tttttaagca atgtggatgt atccagattt tctgtcttag gtttttccgg ttttgcttcc
    42961 ttctcctttg cctccggctt tccctcagcc tgcggaggca tatgaacctt cttttcagcg
    43021 gccttgagtg cttcatccat agtgctgatc ggccttttca cctttgcaga ccttttaatc
    43081 tgtggaatca gccttgccct tgcggccgaa agcttttcct ccactgctgc caggggcagg
    43141 ttcagccttc ttaaggcaat atcaagcata tacaatataa gcgcaagtat aagaagcgcc
    43201 ggggtcaaat ctaccgtccc gaaaatatcc tttatttctc ctttgtatac atcttcagga
    43261 gtattgataa gcgtgcctcc cgcttcctta atcagtctgt ccaaatagcc cctctcaggc
    43321 tgtattctgt attcgggaga gtattgcacc gatatccctg tactggcggc atttatcgtc
    43381 tccccatctt tctgctgcag ggcttttatg atatatgagc ctgtctctgt tatttcaaag
    43441 tcccccctgt actgccccgg tgcaacagga tagagagtaa tctcactgct ctccagagaa
    43501 ggagacacta ttgaggcttt agactcaaac tcacccttat ccgccttgtt gttaagagtt
    43561 atagtgccct tccctccctg cacttcagcc tctacagtta tatcgtcctc ccggtatttt
    43621 tcaatagtcc agttgataag attctgccac agctttatgt tattctccca gcctacataa
    43681 ttcctgctcc atcttccgtt tatatcggag ttccatgcag cagtcttacc caggccatac
    43741 tgccatactg taagtatcgg atcctcctgg tcgcttgcca gcaccacccg ggaggcactc
    43801 ttggggctgg atgccacata gcccagaagt gccggcaatc cttcctcggc tgctccttcc
    43861 agcaccggat gagtattggt tatagcaggt acaaattccc tgttattaag atacgccctt
    43921 gcagccataa aggtctcttt tgcgaatatc ctcggaatgt ttgcaaactc atcagtataa
    43981 tagaagcgtc cgttggctcc ctttgctata tcctgaagga gctggacatc agagccctgc
    44041 cctaccgcta cggttgatac cgtaatattg tccccggcaa gcttctcaac aagttcagtg
    44101 tatccggtcc tctcagcctg gccgtctgtg agaagtatta tatgctttat ttttgtatcg
    44161 gttttcttta ttgaatcata gccttcctgc aaggcaggga ttatgcttgt tcctcctccc
    44221 ggccttatgg tacctatgtc atcccttatt ttttccgcat catccgcctt gcgggtggga
    44281 accacccagt agttggtatc gtcaaaggtc agcaccccta tctgatcctt gggtcttaag
    44341 gactccaggg tccttgccgc agcttccttg gcaagatcaa gcttcattat tcccccccgg
    44401 ccttctgtca tgcttccgga tttgtctatt acaaggacaa tggacatatc cggtatttcc
    44461 ttcctgccct ttagctccat atgtaccgga agcacctttt ccaggggtgt tttgtaataa
    44521 cctcccaaag cgaaggaatt ttccccgccg gcagcaataa agcctcctcc catatctttg
    44581 acataggaat ccaaagaatt cagaaagccg tcgcttaggt tttccgcgga tacattgcag
    44641 gtaattattg acttgtatgc agacaagccc tccaaggttc ccggtacacc ggcagcatta
    44701 actcttacgt agttcatgcc ggtagcttca agcatcctgg ccacctcatc cgcctctcct
    44761 gccacgtcct caacaatcaa aattctgggc ctgtcaagta catttgtaaa ggttgaagcc
    44821 tcattattcc tgctttccgt gtcggtatcc ggctccagaa gcgccttata gctcttgaag
    44881 cctcctgttt ccgccgtatc ccggaataca aacctgttgc ttcccttctg cagcgctacc
    44941 ttctcttcgg cagccttatc ctttccgctg ataagtgtaa gttttgcctc tgtctgaaca
    45001 gtgctttcca cagtcactat aatgttaaat tcttctccga ctctcaggct tttggggaca
    45061 gttacactct ctactgcagc ctctttgccg atttcacggt ttatcctgta taccttgaaa
    45121 tcaatatcct gccctatcag gctggaagcc agctttgagc tgttaccctc attctcctcc
    45181 ccatcggtta tgagtactat tctttttttg ctgtcctggg gcagtaatga tactgatgta
    45241 gtcaatgccc cttctatatc tgtataaatt cctcttggct cagtttcaat tttattaaaa
    45301 accgaatccc cggatacaaa gctctcaatt agagaattat ccccaaaagc cacaacccct
    45361 gtttggtcct tggagccctt cttttccagg gctttcctta caaaatcctc ggaggttgtc
    45421 ctgtattccc tcatgctgtc tgaggcgtca atcacaaata ttgtggtggt tgtatccaca
    45481 atccacttaa tattcacacc ggttagtgca agtatcagaa gcacaaatac actgttcctg
    45541 agaaacaata tcagccttct tctgagccta tacatttttc tcagcttacg ggcagtgaaa
    45601 aatacaaaaa atattgccaa tggaataaga aaaagcataa gaggtctttc aaaatcaata
    45661 accacgtata tacaccaccc attctatagc tgccgccatc agcannnnnn nnnnnnnnnn
    45721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45781 nnnnnnnnnn nnnnnnnnnn nnnntccttc tcggtaagct ccgcccatat atatggggca
    45841 ttagcggata ttccctcaaa atataccgac attgtctcac ctgcaggtat ttcaacattc
    45901 tttatatcca tcaccttgtc atttccataa agtgatattt cccttttgag agaagtattt
    45961 gacctgttgg atgctcgcac cagtaccgtc agctttccgt tatcatcgga atacgaaata
    46021 taatccaggc taacattcac aagctccgat gcaagaggca ccacctctgc attcaaatcc
    46081 tcggtattta ccggactgtc ggtatagaat acagccttat agctgtcact gccctcatac
    46141 tgcttgatca tggcccttac aaggctcaca ctgtcattga tgtttccaga ggaattgccg
    46201 ggattaatac ctctcagctt gcttattgcc tcccccttat ctgttgtctt cccaatttca
    46261 accctgggac tcctgcccac cgtcaaaagg gttatattgg cctttgtccc cgaacggctt
    46321 atcatctctt ccgccagctg ctttgcttta gccagtcttg tgccattctc ataccttgca
    46381 ttcatgcttc cggtattgtc tattacaatt accagattgg aatgcatacc tcccccggta
    46441 tatagatagg gatcggaaag cgcaaaaacc aaaaaaagaa tcgataaaag ctgcaaaaag
    46501 aggagcaggt ttttcttaag cttctgccaa ggggtattga cttcgatatc cttcaatacc
    46561 tgctgccaaa ggtagatgct cgaaatctgc ctctcttcga atttctgctt caatatgtac
    46621 ataagcacta caaggggcat aaaggataaa aaccacaatg cccaaggtga aaacagcgtc
    46681 ataaaatcac ttcccaataa tacccgctct ggtaaattcc tcaagaacta tcttctctac
    46741 gggttcttct gatgaaacct gtatataggt cgcacccatt ctgctgcaaa attctttaat
    46801 actgtttata aatgaattca gctttttatc atactgatta agtaaagccg gagtaacgga
    46861 tatgtccctg gtctcaccgg tctcgctgtc taaaagtctt atttgaccct caatttccgg
    46921 gctcagttct tcaggtgcca atacatgcac cagaatcaca tcctgctttt tatataacag
    46981 gtattttatg gcatcctcaa ttccaccggt cgtaaaaaag tcagaaaaga ctattgagac
    47041 tccgcttccc ttgaactctt tcttcttaat acaggaattc aaatcggtga tgccgtcaaa
    47101 ttgcatattc tccagaaaat ctgcacattg ccggaacatc cctctgccgc ttaaaactga
    47161 agactgcttg acaagtgttc ctgccaagct gttcaggcat actctgtcca ggttgttcaa
    47221 tgccaggtaa gccagtgccc ctgaaagctt caaggctgac agggacttct ttggctctcc
    47281 gaaattcatg gatctgctgc tgtcaacaaa aatattgacc attgcctccc gctcttccat
    47341 gaaaagcttg acaaaaagcc tgtcgaacct gccgtaggca ttccagtcta tacgccggaa
    47401 atcgtccccc agggagtatt ccctgaaatc ggaaaactcc acggaacttc ccttgctgcg
    47461 ggacttcctg ttgcctcccg aaccggtatt caacataatc cttacgttta tcactattgt
    47521 atcaagtttt ttcaaaaagt ctgaatcaaa gatccgttca cccaaatcaa acacccgcta
    47581 tcccttgata tgccttgcag gcacaatatc agcacttatt tcatccaaaa gctggtcaat
    47641 taatccgtct gtggtaattc cgtcagccac ggcatcgaag ttcaggaaga acctgtgcct
    47701 cagagaaggg tgcgcaacat atttaatatc ttcaaaggag acattgtatc tgccttccat
    47761 gagagccctt gccctggatg cggtaattat tgcctgagcg gctctcgggc ttgccccaaa
    47821 ccgaatatac ttctttgcac cttcaggtgc cccttcaatt tcaggatgtg ttcccaatac
    47881 caccctgagg gcatattcct gtacgggctt ggctattggc acttctctta taatcttacg
    47941 catctccagt attgactccc catccacaat cttattaagc tccacttcct tattggaagt
    48001 cgttatgtcc attatgccct tcagttcatc gaagcttggg aacaaaacgt ttaatttgaa
    48061 tagaaaccgg tccatctgcg cttcgggaag ggggtatgtg ccttccatct ctatgggatt
    48121 ctgtgttgcc agcaccagga aaggctgggg aaggctgtag gttacttttc ccaccgtcac
    48181 cgtctgctcc tgcatggcct ccagaagtgc ggactgggtc ttgggagtag ccctgtttat
    48241 ttcatcagcc agcacaagat tggcaaacac cggccccttt tggaactcga aacggctgtt
    48301 gccgctgtca tcttttacaa ttatgtttgt ccccataaca tcggcaggca tcaaatccgg
    48361 cgtaaactgt atcctggaaa aggacagctc cattacttta gacagggtct ttaccaattg
    48421 ggtttttcca agccccggaa ccccctcaag aagcacattc cctcctgcca ggattgaaat
    48481 caatacctgc tttactatat ccttctgtcc gattattgct ttcccaatct ctgcttcaac
    48541 attattgata acatcaataa tatctcttat ctgtttttca ttaatctcca tatttttatc
    48601 tcctttttcc ttggatagtg ctttatcccg catcattctt tcagtgaagt gaagtattcc
    48661 tttaccatat ccttcattcc cgggggtatg tctgatgcac taatgctttc catcgctctg
    48721 tccttatacc ggccgattat ctgattatac ggcaccgatg ttccccgaac cgtcctgctc
    48781 ttgtctgtta tgacctgttc aacagtgcct cccgtacctt tctttccgct gaggttggag
    48841 gttgtgccgt ctccccctaa tgtctgcggg gtaaatatct tctcatactc cccgtctttt
    48901 ttcactgagc cgtccttttt actgatgcct gagctgccgg aaggtatggg ggcctgatcc
    48961 tcattaccca tgtccgtacc gcttccggct ccgcttccct gtccggatcc ctggccgttt
    49021 ccaaccggct gattgccctg gccttgccgg ccattnnnnn nnnnnnnnnn nnnnnnnnnn
    49081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49141 nnnnnnnnnn nnnnngaggc aagcttctct gccagccctg caaatgcctt gcttaactcc
    49201 ggattattct ttatttcctg tgccagcctg gacaattctt ccgcaagctt ctgcttctcg
    49261 accgttgaaa gcttccttaa agcttccgcc attttcctta tattttcctt gaaagccttc
    49321 tcatcaccgt tttttatcat atccgcaagg gcttttgtca tctcattttt tgaaagaaca
    49381 tctactatat tttccaaatc ttcaaccttt gcatattttt ccttgatata ctccagcttc
    49441 ttttcagctc tgccaagggc tttgcttatt tccttctcat ccttggctgc cttaagctcc
    49501 tgcttaagtt ccgcaagcct tgcctccagt tcctttttct gttcttcgga aagcttcgga
    49561 tttcttttta ccttctcaac cagcttgtca gccttcttct gctgttcggt tattttttcc
    49621 tttaggctgt gaagctcctc tgccctttct gccataggat tcggaatgaa accggaaagt
    49681 gcaagaacta tcgtcaaaat aacacatatc aataaatacc tcctgtttgg cttaagaggt
    49741 attttcttct tgtaatccgt tactttaaga tgtgccaggg catcattctt ttcaagaacc
    49801 gcaaaggcgg attggctgcc gataagctca agggcagtga ctgtcctttc ctgcaggccc
    49861 atggaatctg ccttcagtgc agcataatcg ttcccggggg tcctgaaaga ggaataaaga
    49921 aatgcagcca gcacaaccga tgccgctatc ttgaatatgg tcagaaatac attgtagata
    49981 ggaacaaatc ttgcagcaac agccacccct atcccaagac acaatgccaa tataaaccca
    50041 agtataatac tgtttatgat aaaagcggca tgcagcctgt ctcttatgcc cctcaccatt
    50101 cttatcagtt ctttatcgac aggactcata aatatcactc ctcataaccc cataacccct
    50161 atatgctgtc cgaagccttt ttcttttttc ttcctcggaa tcttatataa ctgaatatgc
    50221 tctttcttac cggatttatc cttgcagctg acagtatcag cagcactgca gacaatacaa
    50281 tatcaaatat tatattccca agccagggac tgattgcatt cgcagcattg ggattctggc
    50341 ctgatcctgc agaaagccct aaaaaagcat taatacctcc ataataccca aattgctcgg
    50401 caagaagcga accgaagcct gtcaacgggt ttgaatataa tatcggcaag aatttattat
    50461 aatcccaatt gtaaaaaatc cttatataga atactcctat gaatatggtt ccaaaagcca
    50521 ggaacgccaa ggtgccatat gtaaaaaccg tggaggctgt cgtccttttc aggtgcgcag
    50581 agaaaaatat tccgatacag cctatagtca ccgccgtcac aatataaaag ccgaaaagct
    50641 gcattacctc ctttattgat atccctccga acagaaatac catggagaaa agcggaaatg
    50701 aagcaataat gagcagcagg gtctggctgg tggaagcaaa aagcttcccc gttataatgg
    50761 atataggttt aagtcttgta caaagcacca gatccagagt ctgcttctcc ctttctcccg
    50821 atatggctcc tgctgtaagt gccggaacaa taaacattat aagcatgaat tgaaacacgg
    50881 caagagccgt atatgcacct atggatacct gaggatcaat gtttgagcta taaggatcca
    50941 tgaatgtggt gtagatgata aaaactgcta caacagtaag caccagaagg tataaagcaa
    51001 ttattcccgc tgccttccag cccctcattc ttactttcag ttcctttaaa agcacaggat
    51061 ttatgttcat tgctcatcat ccccctttgt caccttcatg aatacatcct ccagattgcc
    51121 gtccatctgt gcaaaggata caacaggtat tcccctgttt atcaggttag acagcaccct
    51181 gctcatctcc ccgtcgccgc ctccgaagcc cgcctgtatg gtattctcac cgatcacaag
    51241 cttatcgaca aaaggatact ccttcagaat caacaccgca tcatcaagcc tgtctctgac
    51301 cttaagcctc acaaccttct tattataaac ttgctgcatt atctcttcgt tactgccgct
    51361 tatcaccatt ctgccctggt ctatgatccc tatgctggtg caaagctccg ccagctcggg
    51421 aagtatgtgg gagcttatga ttatcgtctt tcccatgcct ttcaggttct tcaatatttc
    51481 cttcatttcg aaccttgccc tgggatccat tccggaagcc ggttcgtcaa gcacaaggag
    51541 cctggggttg tgaaccaggc tgcgggcaag acagagccgc tgcttcatac cccttgaaag
    51601 gctgtctaca tatgcttctc tcttactgcc gaggtctaca agctccaaaa ggtcatcggt
    51661 gattctcttc ctctcctgac ctttaatatt atatattgag gcataaaact ccagatattc
    51721 gcttaccttc aggtcgtcat acactccgaa gaaatcgggc atgtatccga ttttttcctt
    51781 catcctcctg ctgttttgta tgatatccac tccatccagg gttatttccc cggaggtagc
    51841 tcttaaaagc ccgcagataa ttttcattgt agtggtttta cccgctccgt tggggcctac
    51901 gaaaccgaat atttctcctt ccggaacatc cagattcaga tccgagactg cctggaacct
    51961 tccgtacctc ttgttcaaat ttcttataga aagcattatt tgacacttcc cttcacagaa
    52021 atccgaggaa gctgagcatc ggaatcactc aattctattt tcagcttaag catgctgctg
    52081 ccgtccaaat attttccaag gagttctcca tgtatttcaa agtccctgta gtctccctca
    52141 tcccattcct tctcttcatt atcccatata tactgcttaa cacgctgggt atctcctacc
    52201 gtgtactgta tattaatact ctcagctctg atgtcgtcaa ttttgtaatg tacttcaaag
    52261 gaaccccgtc catagaacgt ctttccgtaa tcatcataat tccctgcatc caaattattg
    52321 acaatctcag gcttcagaaa gccaaggggg tattccacgt tttttccgtc cctgaatctc
    52381 agattcacct tggaggtaat gaaggccttt tcatactttc tggtctccct gccgtttacc
    52441 agcagatctt cggctacagg ggtgctgctc caggcaatga gctgtgcctc atagccttga
    52501 tacgcctcat acataaggct atgctccagc acatgccttt tttgaagatt cagcctgaat
    52561 tccgccatct cctccggagt cagcctggaa ttttgattga ctctattagg tccggaatag
    52621 ggatccttgt atattgcatt tatcagttca tatctttgtc cgaaataact gctgggtttt
    52681 acatctatct gctttgtttc cccatttttt acaggcccta caccggcata ttgagtcgga
    52741 gtaattatat agcattcgtc caggtcaaag cctgaactgt tgattattgt tcctgtaaaa
    52801 gaaccctttg cataattcag attactctcc agcctgccgg aaaactcttt accggtctcc
    52861 atagcaaggg tcttcatgct ccatacgccg cttttgtaaa attccagcac cgtcctggga
    52921 gccagggtaa tctttgaatc tattcttcct gaagtatttt cattgtctat aggccctctg
    52981 tagtaattat cgttcattct gagaggctta accttaaaat ctccggctgc ttcaatcctt
    53041 atattatctt tattgggagt gaagacccct gcataaacct tggggcttat aattccgctg
    53101 ttatcaatat ctaccagtga tatgacattt gtgacgggtc cggtcagtct ggttcccgca
    53161 ccggtaagat atacgactcc cgagaaggct atcgaaagca caggtacggt caaccacatc
    53221 agctcccgtc tatccatgcg cttcagtata aaatagctta tgggggcagc cagcaatata
    53281 tatgcaatat atatgaacac catatggaag ggttttggct gaggcagctc cggtatattc
    53341 ctgagcgcat tatctatggc ataaaaatca tctctgacta taattctttt ttgatatatg
    53401 tcactgaaat agtactgcgg cattatgccc acaatcaagt tatccgcaaa ggcgctgttc
    53461 ccgacccatg tggaaagggg ctccattccg aagtcaaagg aggctactcc caccacgcct
    53521 ttgcctttct cgatcttctg cagcaataca aaatcaccgt ctttaatccc tggtgtgctg
    53581 ttattcatgg ttatatccga tacgcttatg ctcattgccg gcccggtctt gctccctgcc
    53641 atctcattca ggcttgaggt tgtgagtgta ctcacttctc ctacctctcc cgtaatgaaa
    53701 tcatccttaa agactgccag ggtcttatta tgggaaggcc cggttcctat aataagcatt
    53761 ccgccctccg acacccacct cttcaaagtc tcatactgtg ctgtactgag ccttgaagtg
    53821 tcataattgt ttatgactat tacattgaat gccttcaaca catccatgtc ctccggaaaa
    53881 ctatcctcat ccagctttac gttttttgtg cttatattgc cgaagctctt catggacacc
    53941 ttatttatat atttaacact gtcaaaatcg tcacttagca tccctatggc ataggtatcc
    54001 gcacttaatc ccggatctat cctgaaggat tttttactta taatgttatc cccttctgca
    54061 agatttacat taatcttggt gttgaataca ttcataggaa cattcatcag gaactttttg
    54121 acggaattcc tgggcagatt tacgtttatc gcatatattg taatactgcc gtattgattg
    54181 ggcatttcaa cctgcagctc accgtttata tcccgaaggc tgttttctat ttcaaaatat
    54241 accggtgcag caaagccaat tttatagaat ttgtcaaagc ccaccttcat atcgattctt
    54301 atttcatcct ttgcctcagc ctgcaagagc tgcatcgcag gccgcccaat aatcaaagca
    54361 agcattagga tgaatgcagc tgctcttaat acttttttca atgcttaacc cccgtatcaa
    54421 gtcaattttt ctttcattat gaacagtcat acccattatt atagacgcaa tgcatagggc
    54481 aaatgtttcg cagtatatga agaatgcgac acgaggacac ggggacgttt ctcccgggga
    54541 catggggacg tttcccctgt cccgctttca gtgggacacg cgaaacgtcc ccgtgtcccc
    54601 gtcgcattat tcttcatatg cgaatttaaa tataattaag tcttgtctga cttaattata
    54661 tttaaagctg attttcgtat ttttgagtct tatatatctt ttcagcctgc ccgaaacctc
    54721 ctgcctggca aaaaagaata atgaaaatgc ctcccataag aatacccaac cgccgacggt
    54781 aagcccctct attaagataa cggtaaaaaa accgggctct gctttctgtc ctgacaagta
    54841 tcccaccgat aagaaagcaa cagccattac aatatagaaa aatatttttt gcctgatttg
    54901 caataactct tttctgatga aatgaacgct atagtcaaaa taattcttta tccccactct
    54961 tgaaagtttt tctttttcct tgtcatattg gtcttcaggc aggtaaaaca tcagttccag
    55021 aggaaattca aggggaatat cggaatcgca ttcttccaga aaacttataa gttccggatc
    55081 cacatccctt ctcttgatcg gagaaggatc ccagccgttg aaaatctcat tgtaatcgat
    55141 taatgagacc tcaataatat acgcatttgt atccggattc atcttatata tctgtttcag
    55201 atagttcttc tttttcttaa acatgaggaa cctccgtaca tggcataaat actgatgtta
    55261 tgtatatatt atcccaatta ctggcttttg taaactttcc ggatgccagg caccatttat
    55321 tctggtaaaa tgtaagtata tattttgcac atgtgaatac aatgcaaagt gtttagtgca
    55381 gaatacacgg aagcggagga gtattaatga atattgatag aatgaaaaat ctgctgtttg
    55441 atctttgcag tattccaagc atatctgaga cacctggaga aattgaaatg gcagaaaaaa
    55501 tctatgaaac agtcatgaat atggattatt ttaagaacaa cccggctaat gccggaatta
    55561 acccgataaa gaatgacccc ttcggaagat actatgtcca tgccttgatg gagggaaagc
    55621 cggagagcaa aaaaaccgtt atactgctga gccactttga tgttgtgaat gtagaggatt
    55681 atggcgctta taaggattat gcattcaagc ctttggaata taccgagctt ctcaaaaatg
    55741 gcagcggcat cagcctctca aaagaagctg agacggatct cgcctcaggt gattatattt
    55801 ttggccgcgg aataatggat atgaagttcg gattagccct tgatgtcgaa atcatgcatc
    55861 agataggaaa taaattggac aatttcccgg gaaatatcct gttgctgtcg gttcctgacg
    55921 aagaaaacaa ttccgcagga atgctggctt ctgttgaaat gctggccgca ttgaagaatg
    55981 agaagggact ggaatatgta tgctgcattg tatcagaacc gcattttccg aaataccccg
    56041 gagacaacag caagtacata tatacagggg ctgtcggaaa actactgccg gtcttttact
    56101 gcgtcggaaa agagacccat gcgggagatc ctttttccgg cttgaatccg aatcttctca
    56161 cggcaaaaat aatagaagaa attgaactta acccggaact gtcggatgta tcggcaagct
    56221 tcaggacgcc tgctccggta tgtcttaaac agtcagacac aaagaactct tattctgtct
    56281 ccactccggc agcagcctac acttatttta atttcataac cgtaacttct tcaccggatg
    56341 aagtacttaa taagcttatg ggcatcagca aaaaaacatt tgaagaagta cttcacaaca
    56401 ttgaaaacaa ggctgaccgc ctccaagccc ttacaggcag caaaccaaag cttccatcca
    56461 taaagcctat ggttattaca ttccgtgagc tttacaaaat gtgccttgaa gttggggaaa
    56521 aagaatttga tgagcatatg gagtcctttg taaagaaatc accggaaagt gatcttaggg
    56581 agttgtccgt tgatatagtc agggaagcac acaaattctg cccctatcgt gatcctatga
    56641 tagtcctgtt cctcgcaccc ccttactacc cccactcggg agcaagggga cagaacggca
    56701 aaatagctga aataacaacg aaaatcataa aaaaggcaaa ctcaaaatat ggtgtgaatc
    56761 tatatttgga gcccttcttt ccgggcttgt ccgatatgag ctatctgggg ctgccggaca
    56821 atatagatgt tgaaagctta aagacaaatc ttcctctgtg gggggacaaa tacacaatcc
    56881 ccttggatgc gatagccagc ctcaatatac catttatgaa tattgggcct ttgggaaagg
    56941 atgcacataa atacacagaa aggatcaata tcccatattc ctttgaaata gcttcaaagc
    57001 ttgtgtttga agcagtattg tcagcccttg atgcaagccc tgaagatttc gggatatgac
    57061 aagtatcata tcccgttttc cagcatgaat attttccatc ggccgaagct tctcctgtaa
    57121 tgtatatacc atatttgagg ggcttcttcc ccttctataa aaacctcaac tccggaggct
    57181 atcgttccgt catcctctgt tatatgccag ctctgcctta cctgcataag cttcctgtca
    57241 gtggccatat agccttccac atgcaaatcc cctgaggtgt accttatata actggaatta
    57301 tcctcagaaa ccacatacat cgtacccttg caaagcatac tctcgacttt tgacctgtcc
    57361 ctctcaaaag ccgcctgtac aaattcacag gattttgaag caatgaattc gcctaattca
    57421 tcaatattca tcacttctgc attttcttgt acctccggct gaatttggat attattcact
    57481 tcgtttaagc cttccggtgc agaatcggag ttttgccccc tgaaccctgt cagtgcgcct
    57541 ccaagacaga gatcggtaac tgcaaggacg agaaacatgg cagcaaaata gttcaaatat
    57601 ctcttcactg tgtccacccc aattattatt gaaactcata gtgaagttat tatttgccaa
    57661 gctgcttaat ataatacaga gtatattatc tatattattg ctttaatgaa tcttttatcc
    57721 ttatccctgg acaaatcaat ataatctgcc ccggacctga ctataggctg ccaaattcta
    57781 ttggggttta tgaacacgac ctcacacagt tcaccgctat tgagttccac gaaatcatta
    57841 atataagaat tggcaatatt cttcagaaac actgtcagca ctttggtatc cagcttgcca
    57901 aaggtatgca tttcaaaatt cttgattacc tcaaaggggc acatcctgtt ccggtaggat
    57961 ctgttggagg tcattgcatc atatatatcg gctattgcta ctatcttggg aaacaggggt
    58021 atgtcttcgt ccaatgagcc tatgggatac cctgaaccat caattttctc gtgatgcatc
    58081 aatactgaat acttcatatt agctgtagca tccaagatat tatccatcat catatatcca
    58141 aaaatcgtat gcttctttac ctcttcaaac tcgcttttcg taagctttcc gggtttctgc
    58201 agaagccttt cggctatctt ggtcttccct atatcatgaa gcaatcctgc tgtcgcaatc
    58261 tcgtcaattt cttcattgcc aagattcagc catttcgcaa ttatgattga cataagcgat
    58321 acgttcagtg aatgggaata tgtataatca tccaaaggcc ttgccagatg aagatagttt
    58381 ataacttcac ttacagtgct gaattccctt attatatgcc tgctgatctg gccgactgtt
    58441 tgtacgtcta cagcagcacc tctgccaatt tctttaataa tgtaagttac ctcgtcaact
    58501 ttggcaacat acttttcttt aaaagctctt attttccccg gatcatagat actgggtatc
    58561 tcttcttcct gaactgcata taaactatca ttctcggtga gtctgaccct gactctatct
    58621 acaccgttcg catggatttt ctcaatctgc cattgatcca gtatagtatt cttgttgaga
    58681 atcaaaaccc ctaaatcatt gaaaatatct tcatatgctg tcatcccggg ttttagtatg
    58741 cttgacggaa gattcatcac tttcacagac tctcacctcc taaatgatat ataggcaata
    58801 tagcaattgc aaattcttgt aaatatctag aattattata ccactttatt acgtcaaaat
    58861 aaacctgata tttacatgat attacaatta taggataatc attattcttt catcttaacc
    58921 tataataaag cttcccgata ctaaaaagca gtccggaaat ctcctcgttc cttatcacgc
    58981 ggctgcatat tgccggtttg tacggcttcg ctctctccat agcagcaaga taacccaaca
    59041 gttggctttt ttcaataata ccatccaaac tgaacaaaga aaaactgtta attcctattt
    59101 cctttaatat cccgatatcc ttttcaaatt cggagatatt ctcatagtat ggttcattgc
    59161 ccattttccc tgcatttgtc actccaattg agacagcaag cttatccttc agcacttctt
    59221 tcaaatatga tatctggtga tacatcaagt aatccacatc tgtcttcctg atctttctca
    59281 gttcatttct aatcatagtt gaataataca taagattgta tttatcccat cccacaccaa
    59341 aaacaggtat ttcaagggca ttttgaatca gctctgattt gaatcgtatg tcataataac
    59401 aatgccttat acaggcggca tagctttcca gaccctttga acgcaagaag cgggcagtct
    59461 cggacaaaat cctgccggct tcagtaaagc gctttctgtt aagatttgca gtaagcattt
    59521 tgccgcatgt tataagaaga ccgataatat ttttaggttc acacaggctt ttaatgtcct
    59581 gtaacggtga ttccagatca atacacagtc cctttatttt caaaccttta tactcaaatc
    59641 tctctgccaa ttccctcaca tatctgtcga attgctccgc atttttttca tttatccaat
    59701 accccatttc atcggaaagg gttgcccata tggataaagg cacatggtgg tcattgtagg
    59761 ttctgaccat atcgtaaaaa ccctcgttgt cgtgcccgta ttccagctta tagttcagga
    59821 ttacattata ccttttgaat gcatcgatta tataatctct gcaaacatcc tccggaggag
    59881 taaattcgca ccatatcctg gtttccatat ttgcctccta tattctgtgt acaaaatctt
    59941 ttgcgtatta gaagaataat gcgacgtagg taacgggtaa cacgggagac acggctgttt
    60001 catggaatcg ctgcgcgatt gcacggtaac ttttatatat tatatagtta cataagaaaa
    60061 tttcgatact tacatcatta tcgataatta tttatgtaac gtaatattta tcccgccgtg
    60121 catttagcgg tttttcgtgt cttcagtgtt atcataactc cgaagccctg ccctaaacct
    60181 cgaacctcgc gcctcgtacc tcgtacccga agaatgcgtc gcattattca tattatgccc
    60241 tgttgcggca aaaacctttg gatttcgcaa atagtgattt ttgccataat gttgtataat
    60301 aatatacagc aaaagacaga gatgcaggtg atattatggc agcaagctat agaatgttaa
    60361 aaggttatgc tgatacttgt ttcgttgaaa agaaatcaag atttatatcc tatgcccagc
    60421 cggtatattc agaagaagaa gcccttcaat ttctagcagg tatcagaaag aaacattatg
    60481 atgctacaca taactgctat gcctatatcc ttggggaatc aatgaacata cagcgatcca
    60541 gcgacgatgg ggaaccttca gggactgccg gagttcctat ccttgaggtc ttaaaaaagg
    60601 aagaactcac taattccata gtaattgtca ccagatactt cggaggaata atgcttggag
    60661 ccggggggct tatcagggca tatacccagg gagcggtcaa cggcattaaa gccgccggca
    60721 ctgtgaggat gcagcccttt gcagtatatg agcttaaaat ggactacagc cttttgagca
    60781 agctgcaata tgagatatcc aagaaggatt atataataaa cagcatagag tatcttgaga
    60841 atgtaacaat gagagtcctt gcagcatcag accttaaaga tgcttttgaa aaagatatag
    60901 cccagtggac aaatggaggt attctgacag agtccctggg cgaacaaatg ctcaaagtgg
    60961 atgaagaaag cggaatgata gttactaatc agtcgtaatt atcataatca aaatcaaaat
    61021 catcattgtc aaagtcaaaa tcatatccgc cgaaggggta gccataggaa ttccttctcc
    61081 tgcgtcttcg ccttcttctg aaaagctcgt tcagtaacag cagacgcaga agatcccttg
    61141 aaaaccttct tgccggatac tgtctggttt cttccccctc cgccatttca ggcatatcct
    61201 tcaccattct gttatatgca ttatccgtca tcatatttat catttcccta tcggggcggt
    61261 aatcgtgtat ttctttatcc ttcccttcca tcctgtcaac ttcaactttt acatatatca
    61321 ttattttcct gcagctttcg ggatacatct tcataaaatg cctgtcgtct tcctcatcct
    61381 cataaaagtt atccatttca atttcttctg ccaacggcat ataaggcatc atatccatag
    61441 gagcaactcc atgcatcata tgcatatcgc acatcatcgg acatttgaac ttttttccca
    61501 tttgacaacc tccaaactta ttcatcataa taccagtaat atgaaacggt tttgggaaag
    61561 tgcaagtctt attgcaatgt gcatccatat ttttacatga aaaaacccat ccttcctcga
    61621 aagatgggtt tgagtcaatc tatttcactg cctttcttat tttttcagct atacccttca
    61681 ttttgtataa aggtaccgag caaattgcaa atcttatccc ccttgaaatc ggtactgcaa
    61741 atatattgtc ctttttcagc agcttgcagc tttcttcagg ttgtgcggca ggtatggtga
    61801 caaaaaatcc tgcgttatac gggcatatat ccagcttgac gctttttgct tcattagtaa
    61861 aaacttccgc ccttttctcc agcagcagcc taacctgttc cctctcttca tccaccttgc
    61921 gcagcagctc tttatcattc ataatgacag aaagcagctt cattccgcat cttgtcccgt
    61981 tggaccaggt acccctgttg gaagcctcaa agacatcgga gaactcttca gctacacctt
    62041 tatcggagct tacgcatacg gcagcccctg tacgaagccc atacatcgta tggctcttgg
    62101 atatactgaa ggctactaca accagaacat tcgacggcaa tccggagaaa agcctcatga
    62161 atctccttgt gtctgaagcc cttcctgcat agtcgatata tgctatgtca caaataagtg
    62221 taattttctt gctttcatcc aaagcttccc tttttattat cataataacc tgctcccact
    62281 cttcatcgct taaagcataa ccggtgggat tctgtgcagg ggaattcagc agaattacca
    62341 agctgttctg tcttgtcagc agttctttga ctttcatttc aaatgcatca agattgaatc
    62401 ttttttcatt gtcaaaaaga gaaaaaacgc ttaacttcct caagttctct tccgcaagag
    62461 tcttatacgg tcctcagtac cagtcggagg tcagtatcgt atctccaatt tctgagtaat
    62521 tccagattgt ttgtcttaaa gctccgctgc cgccgggagt tgctgcggcc tttatgtatc
    62581 cttcaggcct gtattccttg aaggcggcct ggattgccga ctcaagatag tcaggagtcc
    62641 ccagtatagg tgcatatgcc gcaaattcag agtccggaag atttctcagt gtttccagca
    62701 ccgaaggcaa aaccgccagc ttctcatcct catcaagtat agagcctacg gtggcattca
    62761 ctaccttttc cttgccgaac tgcagtactg cattgcctgc ttctctgttt aaagcaaata
    62821 tcttgtcctc tgacctgaca gtattaacat gcttcgcaac catggaaaat gattcattca
    62881 tattttttcc tccagttcta tatgtattga ttatatataa atgtttttat taacatatat
    62941 tatatggtat tgttaatgtc atcatgcctt tcctgtatcc cctcccgttc attgtcatcc
    63001 ccggatatac aggttctgtt cctgcctgag ttctttgctt tgtataacgc cttgtctgca
    63061 gaatgtataa gcatgtctat gttattgcca tgcagaggat atacagatat ccccagactg
    63121 caggttatct tcgggggatt ttcattgctt attgcggtca aggacatttc ctccaccttt
    63181 tttcttagtt tttccgcaac cctatatgct ccgcggtgat tcgttcttgg aagtaccact
    63241 atgaattcct ctcctccata ccttgacact acatcagcct cccgaacatt gaacttcaag
    63301 gtatcggcaa aatacatcag tacataatcc cctgcatcgt ggccgtaagt atcattgaat
    63361 atcttgaaga agtcaatgtc aatcattatt atactcaagt gaaggttatt ctcttttgat
    63421 attttaatct gctcctcaag gtatttttca agataccttc tgttatatgc ctttgtcagg
    63481 gtatcaatgg aagccatatt attgaggctt tccagcagcc ttatgttgct gattacagga
    63541 gtaatgttat ctatgtatac ttttatctct gctatcagat catcattgaa ataattcttc
    63601 atatcgcttt gtatctgaat tacccctgag actatacctc ccagtattat cggaaggcag
    63661 atatagcttg ctgattcact gtagaaatcc ttgcagtcaa aatcattgtt attgccgtta
    63721 tttatgaatt cttttcctga ctttacagcc cagcagttct cttcaaccgt cattgtgttt
    63781 tttatagatt ggatcttctc catatccatg gccttatata atgtcaagca tttgtcattg
    63841 tactcctttt tcgccaccac aacttgagtt atgccgcttt ttttcttcag cacgtcaaac
    63901 aatatttcat atacctcatc ttcattggaa gcttgcctta ccaaatccga aaaccttttt
    63961 atgctgctca gtctgtttga aaactgcttc tccttaaaaa gaaccttctg tgcattgata
    64021 taagaccata taattattga aaaaactgca gccagcagta taactgcaat aacggtaaaa
    64081 tcccttattt gtccaagtgt agtttcagcc aaagggtcat tatgaataat tccctggacc
    64141 cctattatat tcccttcaat atcctttata gccctaaatg caccaaagta tgagtcttca
    64201 tcctcccaac tgcctctgaa aatctcaagc ctcttaacaa atggcgtatc cttttcaagc
    64261 acttccttac ggatatcttc cggcaaactg tttcctttgt ttaatctgta cagctctgat
    64321 tcaagactgc ttgcaataat ccgatccttt gcggatatta tactgtgcat gcctgaatca
    64381 ctcattatct ttccggttat attatcatcc ccgtcaaggt tcattaatgt tacgattccc
    64441 cctatgacac ccctatcatt ttccatcaca ggtactatga catattgaac caaggtaaac
    64501 tcacccctgc tgcctatagg aatcagttcc gtacctgtaa taacagttcc ggatgacaat
    64561 gcttctccta caatctcttg aatctttgtt ttgccggagg cctttatcag caggtctttt
    64621 ccctttcccg ccgaagatat gatattcccg tcctctccca caacatacca ttcatctaca
    64681 tagtccctgt gctctcccca ttttttcagc aaatcctcca aggcagcgct atccttttca
    64741 gtaatcatct ctttaatact cgtttctacc gatgcctgca gcatgcccat tttcatctga
    64801 ttcataaata cgccgtagga gctttctgca aatctcaggt tgttgttcat ggatttaaaa
    64861 acatttgcat aaatgcggcc ggaaaaagca ttgtatatgg aatatccgag aagaatactg
    64921 ataaccagta aagcgcccca taaaagaaga gatttcgtta tatatgactt attatcttcc
    64981 atattgccat ccccctcata atgcatgctt tacatcaatt cattgccttt attaatatat
    65041 cgacagcatt cgacatgata ttaatatcct ctatttcgtc tccaaaagcg aatcgacaat
    65101 caataattct tctgaacctt tacatctctt tttactcctt gttttgtgca tcttaactcc
    65161 ggcccttttt cctccagcgc cgcgagaatt tccttaagct gcagtatatc aactgtcgtg
    65221 ttggtacagt atccgtttgg cctctggttt attacgccca taaccggtat cctgcccaca
    65281 tctgccatac cgctcaacaa ctctctttca catgcaacag caagtataaa atcaggattg
    65341 aattcctttg cggtatttct ggccgctgtc ccgcccttta caactatggc cctgacagca
    65401 cacctttcaa ccaggtctgc agcttctcca atcctgcatc cgccgcatct gcggcaattt
    65461 ctcatgtcat cggttatctt gcagctgcaa tccttgtcct gcatgcagcc ggggagaagc
    65521 ataagtattt tggatgggcg gttcttttga agattatgcc ttaccgcaat attattaatg
    65581 ttaatatata accttctgat atcatccttg tctccgctga ataatcctgt caaataaata
    65641 tataacggca gcagaatacc gattcccgct tccagtacat atatgctgaa acccttcgcc
    65701 ttgcctgttc tgtatgctct tgacaattcc agggataccc cccatattaa cagcaaagac
    65761 accgatacgc cggcaatcat atagacgatt atccttcggt tcgaagcggg atatatcttt
    65821 aatacatata aaaaggatat taatgctgta attaataaaa tactcgttat aattgctgat
    65881 atttgcatca gtttttttat ttccttttcc aaactgcttc ctccatagcg tgatatatta
    65941 gaagaatgat gcgacgggga cgcggggaca cggggacacg gggacgtttc gcgtgtccca
    66001 ctggaagtgg gacaggggaa acgtccccgt gtccccatgt ccccgtcgca ttattcatat
    66061 tatacggatt attaggttta tatattcaga cttggattaa tcagactgcc ttgggtcata
    66121 ctaataaata gagcattgtt tgcagaaaac cggaggaaga tatttaaatg gcactggcat
    66181 gtttttacgg cgctatagca gctgcatgga tcatatttct aatatgcgct ctgaagaagt
    66241 caatatcctt caagcatata atggttgcta ttgccggcat gggattttcc ctgctttatg
    66301 aaacagcatt gggggaatat gccgggctat accactatat caaccaatca gactcactct
    66361 tatatattat aatatcaggt gtatttcttt atccggtgat tgaaatgcta tatgcattat
    66421 tcctgcccga aaggattaaa cctgccatcg tgtacaccgt catatgtata atgcttttac
    66481 tggtttttga aattctaagc ctgtatacaa gaacaatagt gtttacgggc tggaggattg
    66541 ttccttggtc catagcaaca tatatcttaa cttttgtctg ggtgaaccta ttgttcagat
    66601 atctgaaaaa aaaaggcctg tgatcaggcc cgattccttc attgattatt tctcccatgc
    66661 tttttcatca atttgaaccg tgcatagata ttattcttgt cagctctaat tctattccgt
    66721 taccgctcct tccgaatacc tgtttccggc attccaaaga agatattctc caatgccatt
    66781 ctcctcaaga gctcttattt gttcctttac ctgcttgtcc gaatacttga tatgcccagg
    66841 tacccaaggt gcagaaaaat cctgtatcca tggccttatg gccccgggtg tctctatatt
    66901 cttgttccgg gctgcagaat ccttggcgga attgaaaaca gtttcgtatg gatatgcatc
    66961 aggtaccgga agtccataag tgccgttggc ataatggctt ggatacatca tcggacaaat
    67021 atagtcaact gcgttgctga ccgcttccca gtactgtccc agcgacatgt catccgctac
    67081 cgagcctaca aggccatata catcggccga tatatataca ttcaaaggtg ccagctgctc
    67141 ccttgcatat ttcaggtaat cctgtatagc ctgcggtttt gattcccctt cgttattcct
    67201 gtagtccagg gatttgtcca atttccctcc gtccgatgca gggaatctta catagtcaaa
    67261 ttgtatttca ttaaagcctg tttccgctgc ttcctttgac acttccaggt tatattccca
    67321 cagaaccttg tcgtaggcag atgcccaaat caaaccgtcg ctgtttgtaa aaggctttcc
    67381 gctgtccctg tatataatgg ccctgtcagg atgttgtttg gtataagtag gatccttaaa
    67441 ggatactatc ctggcaactg tatatatatt attttcctta agctttttca taagtgcccc
    67501 aatgtccttg acagtagcat acttattggc ttcaggactg tacttttccg cagccttggt
    67561 gggaaaaagc atatgtcctt tgtcgtcctt gacatctata acaaatgtat ttatctttgt
    67621 cctttttgtc atttcaataa gcttgtccag cctgtcattc gaagcagaat atttggtgag
    67681 gtatattccc cttgcttgta ctctgggatt ccccggatat tcataggtct tattttgagg
    67741 tgaaaaatca agcttcttca gattttcatc cagaagctcg gtcctgtccc tgactgtata
    67801 ctcggcagct atccaacctt cattacttcc tgttataccg tcatagctta tcctgtacca
    67861 ggccttcccc gaatcatcca ttttttcttc aactatatct acagcagtgc ctttgataag
    67921 atcatccaat gcctttgcat cagttcccgc cgactctctt accttgagtt tactggcact
    67981 tatgtatacc actccaagct tttcatcttg atctgattct ataggatccg gagcctcgta
    68041 tacctcggta acagttgtct cttggggttg ttcagcgtcg gcaggtatgg gatttgtggt
    68101 tattgccggt gcagcctgat tgtatgagca gccgacagca agcattaata cgaataatag
    68161 tgcaataatt acggcaaaaa acttcttctc tttcataggc acctcacaat ttaaaatata
    68221 ttgcttcata aactaattat agcatgaaat atgacgtttt ctgccatgat attgttgcaa
    68281 tttgtcatgt aaataaatac attttgctaa aaatcttcaa aaaaaaagac ttcatctgaa
    68341 gcctttatac ttctttcata atttttactt ggtgttttcc tacttccaac aggaacctgt
    68401 ctttaagctc ctcgccgttt tctacaaatg ataccgggat tttcttagcc ctgttgaaat
    68461 tcacatccag atccaggcac tcctccttta aagacatctt aataacttca tcaaaaggaa
    68521 gcttgggttt tatacccttt acagtataac ctccccggta tctctgttta acaaaagcat
    68581 aaatcagata ttttccatca ccaacacagt tcactgcgca taaggccaca tcctcaaact
    68641 taaccgtcac cctggtaaag ggccttacta caactatctt cttttcatcc attgttattt
    68701 tcaatatcag gccgctatat gcagtgggga taaacagcag aagcaatccg ataaaaaagt
    68761 aagcccagaa gccgtgaggg actaaaacgt atatacatgt tattaatgct attaatgcag
    68821 ctgtcactat taaagaacct cttatcgctt cccagcgccc cttatactca gtcacattct
    68881 ttcctcctta gctatttgcc gaatccttgg atttcataag atattctact ttattataat
    68941 taatctcttc tgttaaagca ataccccggc aaaatccacg tgccttgctt cctgcttaga
    69001 agtaaagttt tatgattatt atcatccctt tgtgtgattt atatcacatc cataaacaga
    69061 ttcttttagt aaaatccaat taagtcaaaa aatatattat caggaggaaa caatatgttc
    69121 tgtaatcaat gtgaacaaac cccccacggc ggatgcacaa aagtgggagt atgcggaaag
    69181 aatgaggata tagcagcact tcaggataca atgatatttg gcttaaaagg tatagctgcc
    69241 tatgctaccc atgcaaggga aatgggttac atcgaccctg aggttgacgg cataactcac
    69301 gaagctcttt atatgagtct gacaaattca aacttcaatc tgaaagagca cattgacatg
    69361 gttctcaagg taggttctgc cgcagttaag gttatggagc ttctggacaa ggctcatacg
    69421 gcaagattag gtgtaccggt tcctgttaca gtatcacagg acaaaatcca aggccgctgt
    69481 atacttgtta caggccacaa tctttatgcg ttagaggagc ttctcaagca gacagaaggc
    69541 aaaggtatca atatttatac tcattccgaa atgcttcctg cccatggata tcccgaactt
    69601 aagaaataca gccatctgaa aggcaatgca ggtaaagcct ggtatgatca aagaaagctt
    69661 tttgaggaat tcccgggtgc catactggga actaccaact gtctcatgcc aatcagagga
    69721 acatattcag acaaattctt ctcctacgga acagcagggc tggaaggagt acagaaaatt
    69781 attaatgatg atttctctcc cattatagaa aaagcacttt cactgccgga agttgatatt
    69841 gaatcagata agacccttat tacaggtttc catcatcaaa ctgtcctctc aatagcaccc
    69901 gaaataatcg atgcggtaaa aaccggtaaa ataagaaggt tctttgtcat tgcaggctgt
    69961 gatgctccga ccaaaggcag ggactactac agagagcttg ctctttcact gccgtcagat
    70021 tgcgtgctgc tgaccacttc ctgcggaaaa ttcaggttca atgaccacga cttcggaaca
    70081 gtcccgggca caaacatacc gaggtatata gacctggggc aatgcaacaa ctcaggctcc
    70141 gcagcaaaaa ttgccattgc tcttgcagaa gccttcaact gctccgttaa tgaccttcct
    70201 ctgagtattg tactctcatg gtttgagcag aaggccgtag cggtactcct ggggcttctc
    70261 cacctgggtg tgaaaaatat ccacataggg ccaaaagctc ccgaatggtt cacctcaaat
    70321 gtagcggaag tattgcagaa gaacttcaac ctgcagctta taagcggtga tgcaaaagca
    70381 gatttagaga aaatgctggg ataagaaaga gctgccttta cttggcagct tttttttcac
    70441 cctttttctt cactactttc agtctgaaga agtcttccct ttcacgttcc tccagtactt
    70501 ccagtatctt gcccactatt tcctcatatt tgggtatctg aatattctgc agtgcatttg
    70561 tccttttctg agtccttttt acctccattg caagcttgta aaccgagtcc tcaatttccg
    70621 aaagctcata gagcagcagc ttcacctcat ggaatttctg cattgccaca tcaagagccg
    70681 tgttggtatg ataaaagcta taatagtgga taagcttctc ttcctgatga cttatttcag
    70741 gtatttcaac acccattatg cttttatcca gcacttcaaa gcctgatgct tccggtatgg
    70801 actgggcaat ttgctctata ttgcttatac ccatagtagt atttgcagtc tgaagtgcct
    70861 cataggcatc gccgaatact acctctactt tcttctgaat ctcttcagcc ttttcaataa
    70921 agctcatcat ggtcataata agcacatttc tttttttgtc cagcagttca tagccctttc
    70981 tcgaaagctc aagagaattc tgtgcggcta tgaggctggt tttggtataa atgatttcca
    71041 ttgttttcac tacctcttgc catagtactt gtcaagcagt tccggattga tcctgtcaag
    71101 ctccgatttt ggaagaaatg tcaaaagctc ccacatgata tccagggtat cctccatgct
    71161 tctgttcacc tcagagcctt ggttcaggaa cttttgttca aattgtctcc cgaactccat
    71221 atatctttta tccatttcag aaatgtcatc ttcacctatt acctgggcca gtgaccttac
    71281 gtcctgaaca tgagaatatg ccgaaaatac ctgattggcg acatcaggat ggtcctccct
    71341 ggtaaagcct tcacctatgc tttccttttg cagcctggac aacgacggaa gcacatttat
    71401 gggagggaat attgccttct ggtgaagttc tctcgataat ataatctgcc cttctgtaat
    71461 aaacccggta agatccggca ccggatgagt tatgtcatca tttggcattg taagtatcgg
    71521 tatctgagtt atgctgccct tcgaaccctt tatcattcct gcccgctcat aaaggcttgc
    71581 caaatcagaa tacagatatc ccggatagcc tttccttgaa ggcacctctt ctctggatgc
    71641 cgaaagctct cttaaagctt cactgtagct ggtcatattg gtcattatta ccagtatgtg
    71701 catattatga acaaatgcca ggtgttctgc tgcagtcagc gcgcatctgg gcgcagtaat
    71761 tctttctaca atgggatcgt ccgccaggtt tatatacatt accaccctgc ttgcaactcc
    71821 tgacgaatca aactgctttc tgaaaaaatc agcctcatca tgttttatcc ccatcgcagc
    71881 aaatacaacg gcaaaattgc tctcattatc ccctgatatc cttgcctgct taactatttg
    71941 tgccgcaagt tcattatggg gcaatccgtc acctgaaaac agcggcagct tctgtcccct
    72001 tacaagagta gaaaggccgt ctattgaaga tattcctgtt tctatatagt ttcgcgggta
    72061 tcttctccct acgggattaa tagggcgccc gttgacatca tacttactgt ccgcataaaa
    72121 gcctgctcca tctataggct cacctatgcc attaaaggtt cttcccagta tatcccttga
    72181 cagtggtatc tccatcggtt ttcccgtaaa ggaaacactg gcattattga gtgatatacc
    72241 ggaagtactt tcaaaaacct gtattataac tttgtctccg tcaatttgta cgaccttccc
    72301 tttcttatgt ccttcctgct ccacctcaat gtcaacgact tctccaaata cagcctcctt
    72361 ggccccgctg agaattataa gaggcccttc taccttttgc agcttcaaat attttaatct
    72421 cattgtctgc tcacctctct gccgtacatg gttttcaggc tgtcatagaa gttatcaatc
    72481 tcatttatca attcatcgaa catctccggt ttgtcttccg gaacattata tttcatagtt
    72541 gtaatcttgt aaaacaactc atcattcttg accttggata ttggagcacc catttttaca
    72601 cagttatggg ctttatcata cagcctgtct attaccctga gcattccata ctgctttttc
    72661 agggatacat gggcatcatt cttatgatag gcattctgct gaagatagcc taccttgata
    72721 accctggaaa tttccagtat caaccgctgg tcatcgggca atatgtcctc tcccaccagc
    72781 ttgactattt ccatgagctt ttcctcttcc tgaagcagcc tcaatagctt tgccctaagt
    72841 tccatcatat ccggagcaat gttttcttca taccacgaac tcaggtcagt gatatatccg
    72901 ctgtatgact tcaaccagtt aatggcagga tagtgtctgg cataagccag tgacttatcc
    72961 agagcaagga aagtgcttac aaccctcttg gtattctgag taaccggttc cgaaaaatct
    73021 cctcccgccg gtgacacggc ccctattatt gtaatagaac cgtctctgcc gcctaaggct
    73081 tccacatctc cggctctttc atagaattgg gcaagtcttg agggaagata agcaggatat
    73141 ccttcttcag caggcatctc ttcaagcctt ccggaaatct ctcttaaggc ttcagcccat
    73201 cttgaggtag agtctgccat tattgctatg ttgtagccca tatcacggaa atattcggct
    73261 atggttattc cggtataaat tgacgcttcc ctggctgcca caggcatgtt ggaggtattt
    73321 gctatcagta ctgtcctctc cataattgat tttccgcttt taggatcgat tatcttcgga
    73381 aagtcctcga gcacttctgt catttcattt cctctttctc cgacaccaat atagacaata
    73441 atatcggcat cactccattt tgcaagctgg tgctgagtta tcgtcttacc tgttccgaag
    73501 cctcccggaa tggctgctgt gccgcccttt gccaatggaa agaaagagtc tattatcctc
    73561 tggcccgtaa tcagcggttt tgctatggga ttcctgcggc tgacaggccg ggcacctctt
    73621 acaggccaca tctgatacat tttcagctcg tgaagtcccg tctccgtttc cagctttaca
    73681 agcacgtgct caatattata gctgccgctc ttttctgcaa aaaccaccct cccgctaatg
    73741 ccgggaggaa ccataagcct gtgctcaata agcgatgtct cctgtacttt tgcataaatg
    73801 tcccctgagt caaggtaatc tccgattcct acaattattt ccgcatccca cagtttttct
    73861 tcatctattg taatcatacc gattccttca ggtatgaatg ctgtgcctgc ttcgtacata
    73921 ctaagaagcg gacgctctat accgtcaaaa atatacttta ttattccggg ccccagtttt
    73981 accgataaag gccttccagt ggagattata ccttctcctc tcataagtcc tgtggtctcc
    74041 tcatatacct gtattgtggc tgtctcctta tcaacagaaa tcacttctcc gatcaactgc
    74101 ctgcgtccta ccagcaccat ttccctcatc ttaaggtcgt tagcctcagc aaccttaact
    74161 acagggccgt taatatatac aatttttcct ttattagtgt ccattgcctt acacctcatc
    74221 aagcctgcgg gatatgctgc tcccaatcag ctcacgattt tcctcaatca aggatcttaa
    74281 tgtataatcc gcctgtattt cccgtttttc atcctccgca taaaagctcc ctattatatt
    74341 cttttctgat tctctaagct gacatctgtc cttcaattct ccggaggcta tttcctgttc
    74401 gataaatccc tttaaggttc tcaaatccct ctttgtaaaa gcaaattgta ctgaagcggc
    74461 attttcaaaa tcgacggcag ccttcttcag acttgctgcc agatattcct tgtagccctc
    74521 ctcggataca aagctgtcgg ctttccgaat caacatctct gttatttcat ttatggactg
    74581 ctgccttttt tcaagcaaac gatgctgccc ctgagatttt gtctttgcta taagctgctg
    74641 tttctctttc tctgcctttt tctccgcttc ctgcattatc tgattctttc taatctcaac
    74701 ttcctgcaaa agtctgtcct tattctgatt aaagccttcc gcagctttat gccttctatc
    74761 ataagcagtc tcttctatat ccttgaatat tatgtttctg aaaagctcta tcttatcttc
    74821 aaccgatact gacaagactc atcacctcaa atcttcagcc ctatggactc tttaatgtag
    74881 cttgttatat aatctcttct gcgcctttct ccatgcctgt cgggaataat agtaattata
    74941 ggcttggacg cgtccagcct tatagcttta agttcctccg gaaccttttc tgccaacagc
    75001 tccgtcatta gtattattcc gatatcatta ctgctgcatg cttcatcaaa agcagcaagt
    75061 atctcttctc tttcatgaac caacacacca tttattcctg caagcctcat tcccgtcaaa
    75121 gtatctatat tgtcgcttaa taagaaaaat ctcatcgccg ttctcctctt taaaatggct
    75181 gagagcaagc cgttctaaag tgctcccaat atcattattg atacaataag tccataaatt
    75241 gctatacctt cagccatgcc tacgaatatt aatgttttcc ccagtatcct tgggttctct
    75301 gaaactgctc ccaaagctga tgaacccact gcaccgactg catagccggc tccgattgtg
    75361 gcaaggccgg tggatatacc tgcagctatg aagcccatgc ctgcgccgga atcaccttcc
    75421 tgtgcagcat ttacgatgtc aggagtaaga atgaagagaa acaaagctat tagaaacata
    75481 taagaaacca ggctggctct taaaatgttt ctgatctttc tgccttgaat attggctgcc
    75541 gaacggtata atacgactcc cagacctatt gtaaatatga caatccctat tgcaattccc
    75601 aatattgtat tcatttatat gccccctgtc tatatttgtt ttatatttga attcatctga
    75661 ccggaatcat accatctcat ttccggagtc tccttttccg aacaatccaa ccggcttgta
    75721 ttcaactccg tagccggaat agtatttgct gaagagctca tagtactcaa gcctgagact
    75781 ttgtataaac actataagtc cttccaagcc aataataaag atatttccaa taataagaac
    75841 cattattcct gctgtttttg agttcagcat ctcagccatg gcggcaaagg ctatatacaa
    75901 tcccacatga ttaagagcga aagctcctac ccttatgaaa gatattatat tggatgcaac
    75961 ggaaagaatt gtctctataa ccccgaagcc tgcttctata taataatccc ccggagaatc
    76021 ctcatatagc ctgcggcctg taagcatgcc tgcaagcggt tgtttaaaca ccattataag
    76081 cagaagcaca accataatac ctattggaag gcctatccct acagtcagtt cccctgtggc
    76141 ctttcccaat gctccgaata ccagagtcag gaaaaacagg aaacccgtca ggccttccct
    76201 gccgaaaagc ccttcttcta tattcccgga gctgtaatga ttcagcaggc tgtacatata
    76261 gcttatggtt atcagtatga ttcccaatac tattgcacct atgagcattg tatttatatt
    76321 tgccataggc cttataagca gtgccccaat caattcctcc gacccgaaaa tgcttccgta
    76381 taatgctcca aaaatcatgg aactgactcc cagtcttgtc agtatccctc caaagctctt
    76441 atttgctgtt ctataactta tcagcaagcc tgccaaaaga attatcagtc cttgtcccat
    76501 atctccaaac attgctccaa acatgagcat atatgacaag gcaaaaaacg gtgtaggatc
    76561 cttctcaccg tagctgggca tcccatacat acttatcagt gactcaaagg gtttgaataa
    76621 tgaaatattc ttcattttag tcggaggagc atttcccaac cttttatcct ctacatcgtc
    76681 agctataaat ataatacttt cgccgaaata ttgcttcagt tcaatgcgga caccttcaac
    76741 ttcactttca ggcacaaagc cgaacacaaa aaacatgctg ctgcccaaag ctgtttcctg
    76801 ctttagcttt tccgcctttt tttcaagttc cagcagtgta tatgccttga acacagtatc
    76861 actgtacgtt cggctcatct ctgctacgga ttctctaagt tcctcaacct gtcttttttc
    76921 attactgctc ttcgcattta tctgatttat cacatcctct atggttccgc tgaattcctg
    76981 gggcaactcc agttccttat aattcaatga tataaaaatt ctttccgcct cttcctccag
    77041 gctcttgggt gtgatcgaag caattactgt atttccgtta aaaactgctg cctgaaatat
    77101 tattgcgggc acattttcaa tatttagctt aagctttttg aaattctcat tagaaagcat
    77161 taacaatctg aatttcataa aatttaaatt atttatttct tccagcctta aatcagtatt
    77221 cctaagataa ctcacattat tcagatattg ctgcagcttc tcaatattag ctgctctctc
    77281 tgccatgtct tcggaaatat gcctgacatc ttcatgcacc ttcctcaatt ctgccatagt
    77341 attttcatac tcatcgccaa cacataaata atcctcgcat atcttcagct tgagattgaa
    77401 taagtcctca aataatctta ttattccttc gtccccggaa aagtcccttc cggaaatata
    77461 cggcttgata tttaccaatt cttgaagaat ctgcatatgc ttctgcggaa ctgacaggtt
    77521 caggttattg gaattcaact ctgaaatagc attcagtata tgagtgctgc cgtttatcac
    77581 aattagtctt gatactttgt ccaggtctgt aagctttcct attatactag taattttcat
    77641 tctctcaaca gccaatgatt ttcatctcct atcagatctt ccttacgata tatttatagg
    77701 cttgatccgc cggtacttta taccttatgg cttcaattat cgatattatg tcctttacct
    77761 caaactgcag aaatattatg aatgcaaaag cactaataat agaaagagca ttatttttca
    77821 tcatggattt gagttcaaaa tatatatagc gctccatcct cctttccata taaatatcag
    77881 tggttccgtc atgcttcagc ataaaaccat acctggtgga ttttacaatt ctgttgaatt
    77941 cctccagact ttttgaatag cataaagtct tcagcatatt aaaatccatc ctgtaaccga
    78001 tattaatggt atagttcaac agctcttccg ggctaagcct ataaaactta atacccctat
    78061 atatccactg aatattcagc aggtcggcaa taattccctg agcttgctcc aatgccttta
    78121 tatcccgctt ttcaagcttg ctccatttgt tctgcagtat agagtagtaa gacatatcca
    78181 tgaccatctc gaatctgaaa ggattctcgg aaaaatctcc gtcaaccaaa ggtatcaaga
    78241 acttatagaa ctcagagcct tgaagtgcat atatgatatc tcttatatac ttagcctcat
    78301 aaatcttgct tgtatcaatc ctgctgtgct tccctacaaa gacattgttc ctgaaagcag
    78361 ctgcatcctt tccgttatat actgctcttg ctaaaatctt taattcctcg atttcatatt
    78421 ttgcatagag ggttttgaca aagctcttat aatcccctgt gaagtaatga attatcctgt
    78481 caatgctctt taccatcttc tgctttatca ggttctccag catgcctcta tggatagttc
    78541 ccgtgtccac ttctgcaaga acttcagaat aggcagtcct ttctttaaga tatccggcta
    78601 cttctgttac cgattccaat ccaagcaata aggtataatc ctcatccttt aggagttccc
    78661 cctccattga ttttatctta gtattcacag cagcaaacct ggtgatatta cccatcaaac
    78721 ttacctctca attgcaagaa gctgcttcca aatatctctt gcagcatctt ctttgaagtt
    78781 ttcaaaatgg gtctgaagct tatcaatctt taaatttacg gcttcctcca tctcttttgc
    78841 ctgaagcctg ccggcctcaa ttatctcatc tcgtctctgc tttgccttaa taacggcttc
    78901 ctccaactct tcatccaagc ccttaagctc attttcgccc ctcatttcca gttccgaaat
    78961 ctctgcatcc ctttcccgct taagctccac agcccttgag tcgagttcta taagccttct
    79021 tatatatacg tctatgtttt tatccatata tatcaccttc aaactattta ataataatta
    79081 tattattatg cagcatttag aaatgagagt cattcgcgct aataatacaa gtgtttctgt
    79141 ttaagcttaa tatttcaact tttgctcttt tatatccata atctatgtct ttagtttaga
    79201 ttttgctaaa atacgcaaaa agagcaattt agggagcaat tttttacata ctttatttgc
    79261 aaaaaacttg tttattcgca aacagcatga tagaattgtt ataaaatgtg taaatcgagg
    79321 taatattatg gaaatatttc ataacgaata tttttcgctt ttcagcgtta atggggagct
    79381 gtatatttgt gtatatcaca ccgggtacga gattcgcgaa ttcaactctc tccttatgga
    79441 tatgccattt ttacagctca atagttttac aaccctgaaa aatgcattgg atgaggcttc
    79501 caggctcagg atatgtatag gaaaaatcaa gccaagggtg gaagtggaaa tatctgatga
    79561 cgaaatggaa gcatcaataa tgctcaatat ttcggcaaag gaatttgccg aaaacaaagt
    79621 tccgatttct tcggaaataa ttgaggcgct taataatgcc gaggttgttt tcggtcatga
    79681 caacattttc aaaaaaccta ttaccgttca gaaaaaaatt aaagccgcaa agggcaccaa
    79741 accggaagac ggcaaggata ctgtaatcaa gtactatgaa atccccgata agaagcccat
    79801 tgtaaaagaa gacggaactg taaaccatta tgagcttaat ctgattgata atgtcaaaaa
    79861 aggtgactgg ctgggggaga aaatacctgc cacggaagga aaacccggca taaccgtcac
    79921 ggggaaagta ttgcctgccc gaaaagggat tgacttaaca cttaaatatg ataagaaaac
    79981 cgttgaagag catgaagaag gaggcaaaat cgtactcagg gctaaaacag acggtgccgt
    80041 aaaatttgag aatgacaaga taaaggtgga taatcacctc ataataacaa aggacgtaga
    80101 ctatgaaacg ggtaacatat cctttgaagg atatgttacg gttaaaggta tagtaaagga
    80161 cggtttctct gtaacagcta aaaatgatat atcaatacaa ggcaacatgg gcttgggagt
    80221 aataaataaa ataatctcaa aagagggaag catatatata aaaggtggta ttttcggtaa
    80281 gaacatatct gaaatacagg caaaaaagag tgtatttata aagtactgca acgaatgcaa
    80341 aattgtggca ggagaagata tcaatattgg tttttattct cttgacagcc atctcgaagc
    80401 aaaaaaagtg attttggacc ctatgcacgg taaaattatc ggaggttccg taaatgctga
    80461 aatccaagtt gttaccggta taattggaaa cagatcagag aaaaaaacgt atatatccgt
    80521 ctcaggtttt aaccgggaag ctattcggaa ggagattgaa caactctccc aaaaatacag
    80581 tgttactctg aatgaagctg ccaaggcaaa aaaacaaatc gaatcctttg aaatcaatat
    80641 tccgggagct gaatacgtaa acagcaggga ataccatcaa tgtatcagaa aatacgaaaa
    80701 tattctggat gaaataaagc tgctggatga atataggaaa aagctggagc ggattcttga
    80761 aaccaaaggc gaaggtgaga ttgatgtttc aaaagctgca tacccggaga cctatattca
    80821 aatcaaaaat atgcagaaaa aaatcgattc aatagtcaaa ggcagttttt atgcactgga
    80881 taaaaaactt catcataatt gaagaaaatt gcacagcaat tttcttcagg ttgcgaggag
    80941 ggacacgggg acgtttctcc tgtcccgctg aaagcgggac aggagaaacg tccccgtgtc
    81001 cctccccgtg tcctccccat gtatcgtgac gcatcattct tttattttta tcctatgata
    81061 tgtctttttg ccttttttta tgataagctt gccgtcttta agatttgcag ttcctataat
    81121 cagatctatg ctctcaactc tggtatcctc aactaatact ccaccctggt ttataagcct
    81181 cctgccctct cctttggaag gggcaagacc ggtcataacc aaaagatcta ctatgtttac
    81241 acccttatcc aattgttcct ttgacaattc agttgttggt atatgttcag agtctccgct
    81301 tcctccaaac agcgattctg ccgtgttctg agcctttgcc gcctcttttt ctccgtgaac
    81361 catcctggtc agctcatagg caagaacttt tttcgcttca tttatccttt caccgggcag
    81421 gctgcccaat cttcttactt catccatggg caggaaggtc agcagtgcaa ggcatttttc
    81481 aacatcggca tcatcaacgt ttctccagta ctgatagaat tcatagggac tggtttttga
    81541 ttcatccagc catattgccc ctgattctgt ctttcccatt tttttccctt cgctggtggt
    81601 cagcagtgta aaggtcatac cgtaggcgtc agccccctca actcttctta taatgtcagt
    81661 tccgcttatt atgttggacc actggtcatc tccgcctaac tgaagcctgc agccgtactt
    81721 tctgtatagc tccaggaaat cataggactg cataagcatg taattgaatt ccaggaaggt
    81781 aagtccttta tcgtacctgc tcttatagca ttctgcactc aacattctgt tgactgaaaa
    81841 atgaactcct atatctctca aaaagtcaac atagttaagc tttaacagcc agttggcatt
    81901 gttatccata atagccctgt catttctgaa gtccaggaac ttctcaaatt gcttcctgaa
    81961 gtttcttgca ttatgatcaa tcatttcaaa ggtcagcatt tttctcatat cgctttttcc
    82021 gcttggatcc cccaccattg ctgttccggc acccaaaaga gctatcggcc tgtggccggc
    82081 tttctgcata tgagccataa ccattatctg caggaaatgg ccgacgtgaa gactgtctgc
    82141 agtaggatca aatcctatat aaaatgttat acactctttc cccaacagct ctctcaattc
    82201 ttcttcatga ttgacctgcg caataaagcc tctttctttt aatacatcat atacattata
    82261 ttttggtacc attgtcataa taaacacctc ccaaaattaa taaaggccct tcgtccacaa
    82321 agggacgaaa ggctcgcggt accaccctaa ttggccctgc agatataaaa cctgcaaaac
    82381 ccactctgga atacttacat attcccttcc ctgtcataac ggaggaagct tccggcaaag
    82441 gcctacttgc aatgctttca acccgcagtt cagaggtgta tttcataagc ttcagctgcc
    82501 ggcttgcacc taccgccggc tctctggccg tgccatgcat ctgatgcata tgcacgtaag
    82561 cctccgcaga ttacttatcc tcatcattac tgttagaaat atagataaat taattatata
    82621 gtcatagtat tactgtgtca ataaaaagta tacaataaat gcattaattg atatttgtta
    82681 ttatatgcta tacaatagat tatgcataat aatttaaaca tagcttagga gagaacagac
    82741 catgaaaaaa tccctgaaag cagacctggc tttgattctt gtaactatat tctggggagc
    82801 aggcttccct gcaacaaaaa ttgctctcca aaccatgacc cccttttatc atatcgggat
    82861 cagattcgct gtggcgagtc tgcttctttc cctgctgttt tacaggaaac tgcgaagctt
    82921 aaataaaagc cttataaaac ctgcgttgat actgtcatca ttgctttttg caacctatgc
    82981 tttccaaact gtaggcatac agtatactac agcttcgaaa tcaggcttct tctcaggcct
    83041 tgcagtgctt atagtgccta ttttctcaat attctacctg aaaacaaagc tggagctgaa
    83101 aacaataata agcgtagcta cggctaccct gggcctgttt ttgctatctt atacaggcag
    83161 cgattttaac tttaacattg gggattttct aacaatatta tgttcgatat gttatgcctg
    83221 gcagctccta ttcaccggaa cctatgtaca gaagcatgac gcgacattgc ttgccattgt
    83281 tcagttattc ttcgtatcat tgtacggaat ggcctttgcc gttattcttg aacccattcc
    83341 ggtaaatatg tctgtcccca gtttctggtc tttaatgttc tcggcggtat tctgcacggc
    83401 ttttgcgttc tggatgcaga ctacagctca gaaatttacc gccgcatccc acattgccct
    83461 gatttttaca atggagccgg tattcggagc actgacctct ttccttctgc tgaacgaagt
    83521 gcttggtgca agaggaatta tcggcggcat acttatagta tctgcaatga ttatttctga
    83581 atttcagtgg gccaaaagcc aatctacatc ttcttaggct ggaaatatcc acctaaagag
    83641 gatcttacat ccattgtggt aataaatgct ccgcagtcac atccgtcgac tatttcataa
    83701 agcgttttca cctgttttct gtccagtatt atattcagca tatagctttt cccttccttg
    83761 ccttcgccct caataatagt gactccaaaa cctgcttccc tcagactctc taccaactcc
    83821 tccaaggctt ccttcactat tacctggaca acaagacgcc caatggacaa tctctcttct
    83881 atcttgcttc cgattatatt gccggcagca taaccggaac aatatgccag tataagcatc
    83941 ggtttatcaa tatttcctac taccttaccc aacacaataa gataaatcat tacttcaaaa
    84001 aaacccaata ccgaagcaat tcttctctga cctttaacaa gcaatatggt tctcatggtt
    84061 cctattgata catcaataat tcttgcaaaa aatattaata aagcctgtat taggatactc
    84121 acactttttc cccctgtata atctgttaaa taaaattcag caatataatt attaattcta
    84181 cacctttctg ctttcttcct gcattttttt gtctctgcaa aaattggcaa aattagagtc
    84241 aatattttat tgtgtttgag tcaaaatata tatatgaaaa taaataatat tcaattctaa
    84301 attcacctta ggagggaatt agttatgaaa aaatacaaag ccctattttc agaagctgca
    84361 atattacttt tgcttgtttc ctctcttggc ggctgtacct atgacccggc aagaagatac
    84421 atccaaaccg acccaaatac caataacatg gctgcaccga atactccggc cattccgaac
    84481 aatgtggtcg gaagggcaga taataacgct gacggcaaca aaatatccgc tacttatgag
    84541 gtagtggatt tcagctacat tacatgcaat acaaataacc tgaatgtaag agcgggagcc
    84601 ggaaacaaat tcccatccgt cggcacttta aggtcaggac aaaaaatcag agccctcgga
    84661 aaactggatg gctggtatgt agtaaagatg cccgacagcg gcagaatcgg ctgtataccg
    84721 tctgcaagtg caaggcctta cggtacttct gcaaataccg gcactacagg cgccgggact
    84781 gttactcctt ctcctaatca aggtgctata acaggagcag gtgcaggtgc gggtacgacc
    84841 ggagcaggca ctactggcgg agggacagca gcaggatcag gtgccatgag ttccgatgaa
    84901 tcaagaatac tgcagcttgt caatgctgaa agggcaaaag ccggtgcaaa ggctctcagt
    84961 tccagcagtg actgcacaag gctggcaaga atgaagtcac aggatatggc agataacaat
    85021 tacttcagcc atcagtcccc gacttacggc tcaccctttg atatgcttaa atctaataat
    85081 gtaagctata tgtacgcagg agagaatatt gcaatgaacc agtccgccga agcagccttc
    85141 aaggcgtgga tgaactcgga aggccatagg aaaaacatac tgaatcccaa ctttaccgaa
    85201 ttgggtgttg gaattgcacc aaagggtaat ggaagttata tctatacgca gttgtttatc
    85261 ggaagataaa gtaaaaaaaa gcggcttttc gccgcttttt tgatttatgc cgaataagta
    85321 tgctttatct ggcagtctct gtccagctcg ttttcggatg ctgctattat aactgcgcat
    85381 gctgagtctc ctgtaatgtt aatggttgtt ctggccatat ccagtattcg gtcaataccg
    85441 gcaaccagtg ccaggccttc aagaggcag
//
LOCUS       DAOOAB010000003        84270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_308_length_84270_cov_16.461046, whole genome shotgun sequence.
ACCESSION   DAOOAB010000003 DAOOAB010000000
VERSION     DAOOAB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 84270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 84270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84270
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_84270_cov_16.461046"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..370)
                     /locus_tag="PKU88_01125"
     CDS             complement(200..370)
                     /locus_tag="PKU88_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67925.1"
                     /translation="MDQNDNNKELEDKLNDISLYGEFMSTYNYWVSSEEQKNKAQHLQ
                     SIGKPKGTKNRK"
     gene            complement(392..628)
                     /locus_tag="PKU88_01130"
     CDS             complement(392..628)
                     /locus_tag="PKU88_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67926.1"
                     /translation="MVERMISVGGNVTLNDVRRIVDTVKHMDSNEQLVVSMNSQDSYN
                     AQNIFSVLEKNNFEWSTKGGHGGRDYYIIARKKL"
     gene            complement(933..3014)
                     /gene="fusA"
                     /locus_tag="PKU88_01135"
     CDS             complement(933..3014)
                     /gene="fusA"
                     /locus_tag="PKU88_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPX67927.1"
                     /translation="MKDYKTENLRNISLFGHGSAGKTTIAEAMLFNTGVLDRFGKVDD
                     GNTVTDFDPEEIKRKISISAATAPCDWKECRINAIDTPGYFDFVGEAVASLKVVDGAV
                     IALDSIGGVEVGAEKAWDFVNEGKKACVFFVNKLDRENASFSKVVEQLRGVFGNKIVP
                     FALPIGSEASFKGIVDIIDMKAKIREGNKTVEGDIPAELKAEAEEYRNAIIEAAAQTD
                     EELMDKYFGGEELSREEIIRGLRLGVVAGDVAPVLCGAAIKNIGIDVLMDAVMNYMPS
                     PADAQAPEAVNPKSNEKVKIPADVNAPFSAQVYKTVADPFVGKISMFKVMSGKLTADM
                     EVLNSSKEKKEKLSSLFLLRGKKQVAADKLVAGDIAAVAKLQFTTTGDTLCDANNPVV
                     FEPIQFPAPSISMAIEPKAKGDEDKIGNGLQRLVEEDPTLKVEKNTETKQTLISGMGE
                     QHLEVVTKKLQNKFGVEVVLKDPKIPFRETIRKSAKAEGKHKKQSGGHGQYGHVWVEF
                     EPILDGSADFEFVDKIVGGVVPRQYIPAVEKGLRECIEEGVLAGYPVVNLRCTLYDGS
                     FHPVDSAEMPFKIAASLAYKKGMAAASSVLLEPIYHVEVLVPDEYMGDIIGDLNKKRG
                     RILGMEPCGKMQKVSAEAPLAEMFKYATDLRSMTQARGSFTMEFERYEEVPAQIAQKI
                     IEAANAEREKE"
     gene            complement(3217..4890)
                     /locus_tag="PKU88_01140"
     CDS             complement(3217..4890)
                     /locus_tag="PKU88_01140"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67928.1"
                     /translation="MNRYMVLTGVMLKNFNLLNLRPGKSGKNPIRGILLSLLIFAAFL
                     PMILGIGSLAMVGYEVLFEIRQEGLILVTGFSISSLTIFFFGIFYVMSVFYFSKDIES
                     LLPLPLKPSEILAAKFTVAMLYEYLTEAIFLGPILIGYGIASKAGLLYYLYGTLLFLA
                     LPVLPLVYASAISMLVMGFTNLAKNKDRFRIAGGITAMFLAVGINAFVTKLTSSTTSA
                     LELQKLLEQGNNSYAEIISGIFFSNRFAVLALLNSEVLKGFVNLALFMLICMLFVILF
                     LSFGEGVYYKGVVGISEASVKRKKFSETELERYGRQKPVLRAYTIKELKLLFRTPVYF
                     MNCVLTSLIFPLFIILPFIVQPDAMKELQGLSIYLSGERGSAIALAAAFGISVFVTST
                     NGIAASAISREGTNIFVCKYLPVSYKTQITAKVLSGMSIGMVGTLSMLLTASVFITVP
                     AYLMRLIVVVSIFGIAFSSFAGILIDLNFPKLIWDNEQKAVKQNFNVVLNMLVSVLVA
                     GIPIFTIIALHFTLWMTLLTLVLVFGILDLLLYNILSTIGVRLFERIEV"
     gene            complement(4883..5608)
                     /locus_tag="PKU88_01145"
     CDS             complement(4883..5608)
                     /locus_tag="PKU88_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002120909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX67929.1"
                     /translation="MIELINVNKSYSGIVKAVDDLTLTIPEGQITGFLGPNGAGKTTT
                     IKIITGVLNADSGSITINGKNIRENPLEAKRQFGYVPDNPDMFLRLKGMEYLNFMADI
                     YNVPAELRRERIKGLAESFEMEAALTDQIQSYSHGMRQKIVIMGVLIHDPSVWILDEP
                     MTGLDPKSSFTLKEMMKTQAAAGKTVFFSTHVLEVAEKLCDRVAIINKGKLLFYGTME
                     EMRENFRSNGSLEKIFLELTENE"
     gene            5843..6868
                     /locus_tag="PKU88_01150"
     CDS             5843..6868
                     /locus_tag="PKU88_01150"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPX67930.1"
                     /translation="MKNAVNIEWNKLGFSYIKTDYRYISYWKEGSWDNGKLTSDNTLT
                     ISEGSPCLHYGQQCFEGLKAYTTKEGKIQLFRPDQNAKRMIKSCKRLFMPEVPEEKFI
                     DACIQVVKANEAWVPPYGTGATLYLRPLVIGIGDNIGVRPATEYIFTVFCMPVGPYFK
                     GGLTPLDFSVSDYDRAAPQGTGAAKVGGNYAASLLPHEQAREEGYADCIYLDPATHTK
                     IEEVGAANFFGITKDNRFVTPISDSILPSITKYSLLHVAKEYLELETEERDIYLDKLD
                     ELAEAGACGTAAVISPIGSITYKDKKHVFHSLTEVGPVTKKLYDTLVGIQFGDVKAPE
                     GWIVEVK"
     gene            complement(6915..7829)
                     /locus_tag="PKU88_01155"
     CDS             complement(6915..7829)
                     /locus_tag="PKU88_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPX67931.1"
                     /translation="MPLEIICNDITKMRVDAIVNAANSRLQQGGGACGAIFAAAGADK
                     LRDECNRIGYCPVGSAVITDGYALPAKHIIHTVGPVWQGGGYNEEKLLSSCYTNSLEL
                     ALKHGLGSVAFPLISSGIFGYPKDEALRVAVSAIGSFLLKYDMMVYIVIFDRAAYKIS
                     DKLFKPIKAYIDEKYAEKHTSRINPDDVGNRIGESFTEMLLRLIDESGKADPEVYKRA
                     NLDRKQFLKISSNLKYKPGKNTALALAVALELDLDRTADLLRRAGYALSPGSRFDLIV
                     EYFIREGNYNIFEINEALFAFDESLLGA"
     gene            complement(7855..10782)
                     /locus_tag="PKU88_01160"
     CDS             complement(7855..10782)
                     /locus_tag="PKU88_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPX67932.1"
                     /translation="MQFKVGDIYHGFRLVEENDIKETNSKVRLFRHEKSGARLFYMEN
                     EDDNKVFSVTFRTPPRDSTGLPHILEHSVLCGSRKFPTKEPFVELVKGSLNTFLNAFT
                     FGDKTMYPVASRNDKDFENLMDVYLDSVFHPNIYKYPEILKQEGWHYELEDTSGELKY
                     KGVVYNEMKGALSSPESVLSRKLQETLFPDGPYGYESGGDPEVIPELTQEGFISFHKK
                     YYHPSNSYMFLYGKLDVLGRLKFIDEEYLKGFEAIHVDSAIPVQKAFEEMQSYELEYP
                     ISAQEDERDKTYLSLNYAVGLSTDPELYIAMEILEHILLATPASPLKKALIEADLGRD
                     VFGKYDNSILQPVFSIIVKNSNTEVLEDFKRVVSNTLEGLVRNGLDSQLAEAAVNLKE
                     FQLREADFEGYPKGLIYNIKIMDSWLYDKDPTMHLRYEHIFEKIRAEFNNRYFEGLIE
                     KYLLKNNHSSLLVLKPKKGLSEEKDKETKEKLRSIKAKMSEEQLRELVKETGELKKWQ
                     NTPDTPEQLALIPMLSIEDIDRKGEVLPQQVKEEAGVKVLAHHMFTNDIVYAGLLFDT
                     TAVPQELLPYIPLLSGILGRISTENYSYGELSKEIDMHTGGIRFSTQVYGEKDDDSKY
                     HPVFSIRGRALASKTEKMFELISEIVARSKFDDSRRIKEIIQENKSRIEMRISNEGYT
                     IACKRLFSYFSQEGSYLEAISGLTYYRFIADIERNFENRIEKVKENLQKLKRLIFRKE
                     GLIAGVTCEEDDYNRFAGGLGLVLKDLSNEKHPAFKYAFKVESLNEGLMTQGKVQYVA
                     QGYNFIKLGYPYTGKLQVLKTISRYSYLWNRIRVQGGAYGAFSGFEKNGNMFMVSYRD
                     PNLKETLKVYDEMYDYMRNFQIEEREMTKYIIGTISKLDLPLTPYMKGERAIENYMRK
                     ITSEDLQKEREEILGTRQQDIRELSDMMFELIKRRSYCVLGSEIKIRENSELFGSLVE
                     VFE"
     gene            complement(10869..12692)
                     /locus_tag="PKU88_01165"
     CDS             complement(10869..12692)
                     /locus_tag="PKU88_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX67933.1"
                     /translation="MGKDTKQIAIIGGGYGGVHAARLLGKAARKGADISVLLADKKPY
                     HTLMTELHEVAGNRVEAESVQIDLREIFGASGVRVATEEIKSIDFKGRKLESESESYS
                     YDYLIIDTGAEPAFFGTPGVKENGFTLWSFEDAIRIREHVKNMFDKARTEKDHSRRRA
                     MLTFIVAGAGFTGIETMGELLEWRKELCREYEIDSREVSLMVVEAMGSILPILNERLI
                     KKSERYMKRHDVEIITSAPITEVKKESIILKDGREIPARTLIWTCGVQGSDFAAKTEL
                     THGGRGRIQVNEYMQSVDYQNVFAVGDNAFYEEPDGKGGKKPIPQIVEAALQTAETAV
                     HNILASIDKRGKKPLKSNYHGFMVSIGGRYAVANIGGRSMDGFFAMALKHLVNIHYQY
                     GVGGFARIWDYIMHEFIYVREGRSILGGHLSARTPILWLAILRVYLGVIWLIEGVKKI
                     NEGWLDPGKSFIAAMPRPEDVPLESTDAVTNATQVTQYYPEPLLKEPPRVFQWFMETF
                     IEPNAYLFQVLIVLTEAAIGLALIAGLFTILASLGSIFLCLNFILSAMAGSEIFWYIF
                     AGIAMFGGAGRAFGLDYYVIPWIKHRWKRTAFARRTHLYVR"
     gene            complement(12849..18836)
                     /locus_tag="PKU88_01170"
     CDS             complement(12849..18836)
                     /locus_tag="PKU88_01170"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HPX67934.1"
                     /translation="MVKRIISFLLVMSMLLLALPTAVLADSLKQEPENEYIDVNKADW
                     FYESVMYCLENGIFGGTGEGTFSPGTSLTRAMYVTVLGRVAGVDTSQYNTSAFKDVAV
                     GAWYAPYVAWAAENGITHGTDNDTFSPDEAITREQMATMTVRYFKSCNISYEAERQIA
                     SGPKDAPAISSWALDSVLSLWHAGLLAGDENGNFNPASNATRAEAAAFCMRISKTVKQ
                     QEEKKQPTPAPAPTPASSQGSGNDRDESRSDTKYTITFVTNGGDAIDKLRLKPGTALD
                     NLPVPYKMDAVFLGWYKDSSLSTPVSSTDIITGNMTLYAKYAASTGFTEELSVPTISK
                     LDQDKTFTIIVVDSTDGMTAAEVKAGMTFDSPSNPGFAGIQVDESSDGEFIVSAIGGA
                     FDEGGTFKLTLDNDNLSFKGEDESTRICTFTIAKSPVMNLSLNKNMKYLSAGQISDLT
                     QNGFSVKSLSIPLVRVAGDGEGLSGIDAEGGTFVYDGGGINVGDTVAIYEGIRPDQRD
                     LDTSGDDAGDIAYVTITAISGTTYSYVKADEKDILFTPDVLPVSTTADTDGSSDNNSV
                     TVPQSVMAYTDAIYADIGLNAATTVDVGDFIAFHTGTFGSADAENAGYAEITSVTVSG
                     DNYIIAYISTTPEQMWEATDLYTTKEVDGDELLENVDIAALEESVERQAIESGFADEA
                     AQYLAEMAMKTDSFRQLNLTAMADTSSYGAMSMLEAFGTGPEVKNLKVNARVFNYLQH
                     LGGSGARVTLTISCDIVFQAAGEDNNIVIRLSGAFEQEVKVDLNVSCETKWTLLDGWF
                     WWAEDTLVTASIDTGTYTSININASIGTFEKGAQYDLSKFQDIGKQIKDIMNGKKPED
                     SAIADTLREKYKAMLEDDSDWVDIYKKEIFTFEQAIDPFHIVVFGMDVDFVVGAKMNV
                     SIGCNFSYESEKRYIFSLYINAQTATSDTIDLRPEQYNFTFYIMGTLGLRAGISIEIK
                     VGLFSLDLASIGIEGEVGAYVQLWGYFYYEASYRQATGLTSKYGGAMYLEFGIYLEIS
                     FVAQAIDNMFEYEATLYENQWPLLTFGSQEDVYDFEYSADKAPTAFMAKQTKSFVLPD
                     SVFSMNYMDMKTGDIESKNYDPSNFDYSTTNSDFTYDPATKTVTVAPGEKDITEGALV
                     LIWKGNPVAFTSVPIARAVPLHWEDLKSAYTISFNSRGGSAVDTIALPYKGVITPPAN
                     PAKAGYVFGGWYKDEALTQSYTFPATMPAEDTVLYARWVPAPNTKYRVEHYKQNLEND
                     LYTLAETEYRTGTTDSVVHVSPKSYEGFSLVPSPEIVQILPDGSTVHVFHYNRNSYSA
                     TFQPNNGKIGADVENTVKFGGKINIPVFTRARHSFAGWSPAVPETMPAQNMTFEAQWT
                     TVGQVAYKVEHYKEGLDGSYALAETENLTDVANQMVTATAKSYGGFSYNRNAPGTVPS
                     GIVTSDGNLVLKLYYTRNSYTLTFDANGGTGGITRQAKFGEPISAPAVAREGYSLTGW
                     SPAVPATMPAENATYTAQWQAVGLIPYRVEHYLENLDDGYILTDIENLSGAEGDTATA
                     STKSYNGFEYLADPPTVASGTIAADGSLVLKLYYMRKDVTLEFLADGGSAGDNELDYQ
                     YGDFFNEFPPGTIEGYAFNGWSPAFMGTAPAEDTTYTAQWTSNISEALDVTTAVYANQ
                     DYTAGGVYWDYEAQTLTLTDVVITSPDSAESGVDNFALKVPDGTTIILVGSNYLQSGK
                     GPGINSYGILCDGDLIIQGPGTLTALGTPNFMSLADQIVCYGIYAAGTITIEDDAAVI
                     AKSTGATGNSCGIFAEDVVITDSTVLASSNESANGIRCGIYASGDIDITNSTVNTPSG
                     IYASGDAAISESTVNALNSGIEYPDDVFNYYGIYGDNVAITGGTVYVTSQSADGYGIY
                     AAAGVEITGSAAVTARCSDFGGPGTGSGIYAGTGSISISGSTVIAAGKDALAMNKAPV
                     LNNAEAFCASTSADGSGGVIYDSGDIATYKYIQIRAD"
     gene            complement(18888..19145)
                     /locus_tag="PKU88_01175"
     CDS             complement(18888..19145)
                     /locus_tag="PKU88_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPX67935.1"
                     /translation="MSKILKVTPNEDYTLLVEFEHGNKIIFNMRDIIKTIPFSSLEDL
                     NRFKNVTVEEKAISWPEPVPGEKSIMPLRLTVDNILFAIRD"
     gene            complement(19268..21739)
                     /locus_tag="PKU88_01180"
     CDS             complement(19268..21739)
                     /locus_tag="PKU88_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX67936.1"
                     /translation="MKKRRTLKRERINRELTNIFKYPLTIVEAPMGYGKTTAVSEFLA
                     LKGVPVIWTSFLSEEDTAHGFWERLAAEIGKFDKAAESRLKSLGVPADTPQTTMAVSI
                     INEMGYKPDTTLVIDDFHLARSMRVTALFRRFVMEMPDDFHIVILTRDTTNLDITELS
                     AKGLCYIVSQNMLRFTDSEIRDYCALMGFTASEGEIRKIGDYTGGWISLIYLIMLGME
                     RGIPVGRNGTINELVEKVLYNAYDERIQRFLLCLSVMDSFTAEQAVFVTQEEKSEETL
                     KKLRRENAFIAFDEAAGVYIIHNVLLDFLRGKQKDEEERAALYRRLGEWYLAQRKYRT
                     AYGYLYRAGESERILALLDNGDTITSDSATFDGALDLFAAMPREMLHKYPLAYLQYIA
                     IILTNGDPDDFQDGVIRLNELREACERAEDPVPHGRDRVLAELYLTQIFAVFNDAKEM
                     AACIEKAQRLLDGDVSFLQKREAEFTLGSPHLLYTCYREPGSLKELTELMASDFPIFA
                     ELADGCSTGCDYVVRAEYALETGNWQAAELDAFKGIYKAQTKEQIGLVICAYFVLIRL
                     YLYKGKTNEALELLRQLRQDVKRTNNLYYNTGLEIVEGYVYGCLTRLNSIPLWLQTGD
                     MSPAQFFYEGLGFKYIVYGKALLLSKNYIKLEMLTEEFARCFAVFHNQLGFLHNQIFR
                     ATAKYPLYGMEVGCAALREAFSMAREDHIILPFAEYAPAVIDMVRHIAHADSRDAYIK
                     KVLEACVHYMESLKHSIQSAVSLSARELEILTLAAEGLKRDEIAGKLHVTAGTVQTHL
                     HNIYLKLEVSGRMAAIKKAQKLKLL"
     gene            complement(22122..22988)
                     /locus_tag="PKU88_01185"
     CDS             complement(22122..22988)
                     /locus_tag="PKU88_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPX67937.1"
                     /translation="MDTVLLIDSCSDLPRSYVDENAIPFVSLVCNFKDKEYKDDFGKS
                     ISYKEFFDAVRSGEMPSTAQVNVYEYTEIFKKYATEGKSVIYLGFSSALSGSLGSAYL
                     ARDMVMEEIKDADITIVDSKSASLGEGLLAYNANEMLKAGASKDEVVSWLETNKLKVN
                     HWFTVDDLGHLRRGGRLSGTAAFLGTLLDIKPVLKVDDEGKLIPISKNKGRKKSIKTL
                     FEMLQENIEAPEEQVIAISHGDCIEEAEYLKEMILKEYNVKDVIINHVGPVIGAHTGP
                     GVVALFFFGNKR"
     gene            complement(23097..24365)
                     /locus_tag="PKU88_01190"
     CDS             complement(23097..24365)
                     /locus_tag="PKU88_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX67938.1"
                     /translation="MSLIKDLISPYKGMPREIYIIFISRIVNAMGCFVMPLMTIIMTD
                     RIGLSNEAAGFFLSLNSMVFPVASILGGKLADTFGRKHLIIIFGTLGALLYMSCGFIK
                     PSMQLIYVILGASTLMFVAGPAHDSLIADLTTPENRAKAYSLSYLGWNMGFAVGPIFG
                     GLLYRRYLPLVFIGDAVTALIATGLIYFFINETIESTKNDITDESRKLEKREKGSIIS
                     VLLRRPILIYFSIIALGYEFSYSQWNFLMPLHSVKNFGEMGAQYFGWIASFNGLVVML
                     FTPILTRLTRKTRGIRIMVHGILLYAIGFGMLGLIYKLQYFFVSALIFTLGEVLLTIS
                     VMPFIVNHTPASHRGRMSAVLTTITGTGYAVGPMAMGRILNFISIEKAWIFIGIFTAM
                     TAVLMFGLEKYEDRENTMREKEGSAVKVQQ"
     gene            complement(24656..26242)
                     /locus_tag="PKU88_01195"
     CDS             complement(24656..26242)
                     /locus_tag="PKU88_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPX67939.1"
                     /translation="MKNLKFEELNLSDEILKAIGNKGYEEATPIQAQSIPYIMEGRDI
                     IGQAQTGTGKTAAFGIPILEKFNQGEKTVQGIVLCPTRELAIQVAEEIRELAKFKRGV
                     EVLPIYGGQPIDRQIKALKRKPQIIVGTPGRVMDHMNRGTLKIDKVRMVVLDEADEML
                     DMGFIDDIVTILKVVPEERQTMLFSATMPKPILELTKRFQKDPKHVMVVHKELTVPKV
                     EQVYFEVKEKTKLEVLSRLIDMYNPKLSLVFCNTKRKVDELVAELQGRGYFADGLHGD
                     MKQVQRDRVMSKFRSGAVDVLVATDVAARGIDVDDIEAVFNYDLPQDEEDYVHRIGRT
                     ARAGRGGRAFTFVVGREIFKLRDIQRYAKAKITLQKAPSSEDVEEVRTNLFLDKVIEI
                     MDEDDLSQYVSIIERLAGEDYTSIEIAAALLKMAVGNKDKEVQHEETDFNDTGAEDGM
                     VRLFINIGRNQKIRPGDIVGGIAGETGIPGKLIGSIDIYDKYTFVEVPREYAGDVIRI
                     MKDNQIKGRRINIEPANAKH"
     gene            26725..27186
                     /locus_tag="PKU88_01200"
     CDS             26725..27186
                     /locus_tag="PKU88_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPX67940.1"
                     /translation="MSLESVRKFFDENGYSDLQIIEKDASTATVELAAKAHGVAPALI
                     AKTMAFGLKDRNILIVVSGDSRIDNRKFKDCFHEKASMLNPEKVLEATGHAVGGVCPF
                     GLKNPMDIYVDISMKEFEAVYPAAGSANSAVKITPDRLQEVTGGTWIDVCK"
     gene            complement(27201..27626)
                     /locus_tag="PKU88_01205"
     CDS             complement(27201..27626)
                     /locus_tag="PKU88_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HPX67941.1"
                     /translation="MRIAVIDGQGGGIGKAIVERIRQKLEDKAEIVALGTNSLATSLM
                     LKAGANEGATGENAIIHNACKVDIILGPIGIISANSLLGELTPLMAKAIAESPAKKIL
                     IPMSKCNIAVAGIEIKPIPSYIEDAVEIVKTMIKDTKVV"
     gene            complement(27659..29194)
                     /gene="cls"
                     /locus_tag="PKU88_01210"
     CDS             complement(27659..29194)
                     /gene="cls"
                     /locus_tag="PKU88_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPX67942.1"
                     /translation="MQKLLRMIFSRVFLVGTAIFIQVFALLIMIWRFSNYFVYFYTFS
                     TLLSIVVVLCIVNGKSNHAYKLAWIIPIMAFPIFGGLFYLMFGGNKSSKRNKRKMQVI
                     TDKMQNVLVQNLKVMQNLEKEDRGAANQSKYIENYSSCPVHTNTRTEYLTPGERKFER
                     MLEELKKAERYIFLEYFIIDKGVMWDTILDILKEKAMQGLDVRVIYDDVGCILTLPYG
                     YDKELERMGIKCCVFNPFVPVLSIRLNNRDHRKIAVIDGRVAFTGGINLGDEYINAYE
                     KYGHWKDASIAIEGDAVWSLTVMFLSMWDFLRKEDEDYEKFRPTYEYTKDPSYNGYVQ
                     PFADSPLDEEAVGETIYLNLIGEADRYIYINTPYLILDNEMNSALCMAAKRGVDVRIT
                     TPYIPDKWYVHAVSQCNYEALIESGVSIYEYTPGFIHAKTFVVDDLYAVVGTINLDYR
                     SLYLHFECGVWMYKTNSILEVKADFLKTLKVCQRITLGCCRDEVLYKRLGRTILRIFA
                     PLM"
     gene            29358..30170
                     /locus_tag="PKU88_01215"
     CDS             29358..30170
                     /locus_tag="PKU88_01215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HPX67943.1"
                     /translation="MNILVTLNSNYITPLKVMLKSLFFNNWRERFSIYIMQSSLTDRE
                     LQQIEDIVINQGHEFHVISIKDEYFKDAPVVMHYSKEMYYRLLAFKFMPPKIDKLLYL
                     DPDILIINGIRELYNTDLTGYLYAAAYHHRGSINKINKIRLKPYDIEAYYNSGVLLFN
                     LNQQRNTIDEKDIFDFVRENADTLILPDQDILNALYSKSIKPVDEILYNYDPRYYNYY
                     KIISNGKYDMNYIIYNTSIIHFCGKQKPWKKNYSGKFHSLYKHYERIALEQS"
     gene            complement(30173..30676)
                     /locus_tag="PKU88_01220"
     CDS             complement(30173..30676)
                     /locus_tag="PKU88_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPX67944.1"
                     /translation="MAVREILLLGNENLYNVSKEVSKEEIDKAKQIVDDLHDTMADFR
                     KKYGFGRAIAAPQINEFFRIIYMNFDDREIALINPRLEFTDDEKFEMWDDCMSFPGLE
                     VRLSRYKKCRVYYKDLEWKDCIFEPEGDISELIQHEYDHLDGILAVQRAIDNKSYRIN
                     KNKAGCY"
     gene            complement(31207..31722)
                     /locus_tag="PKU88_01225"
     CDS             complement(31207..31722)
                     /locus_tag="PKU88_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67945.1"
                     /translation="MKKLLLFALILLLSALMIGCTAAGSQSRENEEKTQQTDEADKEA
                     AANIVEGFGKKLQEVSLLAPKEVLEKSMEESYGDFVTSELIAKWAKDPMNAPGRLTSS
                     PWPDRIEILAIEKSAENEYEVKGEIIEITSTEKAEGGISAKRPITLAVRKIEDRWLID
                     DVKLGDYEKIK"
     gene            31913..32869
                     /locus_tag="PKU88_01230"
     CDS             31913..32869
                     /locus_tag="PKU88_01230"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPX67946.1"
                     /translation="MVLRNALDLISNTPVVALDNITGNIEGRIFAKLEFWAPGLSKKD
                     RVALQVIEDAEKSGRLKPGQPVVELTSGNMGTGLAIVCKLKGYKFIAVISKGNSIERA
                     KMMKAFGAEVVLVDQMPDSIPGQVSGKDLELVEIEAQRLTKELNAFRVDQFYNPSTAR
                     AGEVGVANEIIQQIDERIDAFVDFMGTGGSFIGVTTTLKKHFPDIKCFGVEPENARRY
                     SNPQDIGSGKHKIQGGGYNMELPLMTGDKLKLVDGFITVSDEEAIQAARRLACEECIF
                     AGFSAGANLAAALKLLKVDFKGKNIVILLPDSGTKYLSTDLW"
     gene            complement(32893..34101)
                     /locus_tag="PKU88_01235"
     CDS             complement(32893..34101)
                     /locus_tag="PKU88_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPX67947.1"
                     /translation="MSNFAQRMTAMEKSAKIIRNLFGAMNDPGIISFGGGAPAKEALP
                     IDIVREIANEVIRADKRGAEALQYGPVMGAADLREAVVNDLLAPKGVAASVDNIMITS
                     GGLETMNLMCQLFIDPGDVILVESPSFVHCVEIFEMFQANCIAVDMDDNGMIPEDLEA
                     KILKYRPKMVYVIPTFQNPTGRTLSLDRRQKIAEMGSRYDVIILEDDPYRDIRYSGSD
                     LLPIKAFDQTGHTVLANSFSKIFSPGSRLGYALANDEIISRLFDAKTATNSHTSMLPQ
                     VICAEFFKRGYYPAHHKMICDLYRERRDIMIECIDKYFPEGTKRTFPDGGLFTWAELP
                     EGINTTELLAEAVTNPDVKVAYVAGEGFFTEGNGAGSDCMRISFGSAAPERIRTGIER
                     LGRLICSKLK"
     gene            complement(34157..34645)
                     /gene="ybaK"
                     /locus_tag="PKU88_01240"
     CDS             complement(34157..34645)
                     /gene="ybaK"
                     /locus_tag="PKU88_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HPX67948.1"
                     /translation="MEKVKTNAMRLLEKAGIKYNTYAYEHSDGLIDGVSVAGKIGCPV
                     EKVYKTLVAQGTSREFYVFIIPVAAELDLKAAARSVGEKAVEMIKVADINKVTGYIRG
                     GCSPLGMKKQYKTVIDSSCKAMDTIIVSAGKIGHQIELAPEDLIKLTGCRTEAVCARG
                     NL"
     gene            complement(34701..39008)
                     /locus_tag="PKU88_01245"
     CDS             complement(34701..39008)
                     /locus_tag="PKU88_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPX67949.1"
                     /translation="MSNLFHVGIDIGSTTVKVVVLNENCDMVYSRYERHYADIKKKLQ
                     QMLYDAFRKLKNSQLTVMITGSGGISIANSLGVSFTQEVVASTKAIKTYYPETDVVIE
                     LGGEDAKITYFADGIEQRMNGTCAGGTGSFIDQMASLLRVDAEGLNELAENYKVIYPI
                     AARCGVFAKTDVQPLLNEGVAKEDIAASVLQAVVIQTISGLACGRPIRGNVAFLGGPL
                     YFLSELRERFIDTLKLKENQVIFPENSQLFVAIGAALSSLNEKPVFFESLIGSLKSHE
                     SASGGESERLERLFSDEKEYEEFRSRHQKSSVIRRELSTFEGECFLGIDAGSTTTKIA
                     LIDDEGAILYTYYSSNEGSPLKSTVKALKELYRLLPKNARIVNSTVTGYGEGLLKAAL
                     SVDIGEIETISHYKAAEFFCPGVDFILDIGGQDMKCLKIKNGVIESIMLNEACSSGCG
                     SFLDTFANSLGMSVEEFAKEALSAENPVDLGSRCTVFMNSKVKQAQKEGATVGDISAG
                     LSYSVIKNALFKVIKVRNLNELGEKIVVQGGTFYNDSVLRSFELLSGREAIRPDIAGI
                     MGAFGSALIARERYQKGMHTSLLGEHELNEFNLETVVSRCGLCSNNCLLTINKFGNGQ
                     SFISGNRCERGTGKIKEQNLLPNLYKYKYERVFRYKPLDSSLARRGTVGIPRVLNIYE
                     NYPFWFTLFTELGFRVELSDRSSNKLYEKGIETIPSESVCYPGKLVHGHIMNLVEKGI
                     KFIFYPSITHELKEQKKADNHFNCPIVVSYPEVIKNNIDVLREKGILFMKPFLPFDDE
                     DRLVDRLHKEFKMFKISKMEIKESVYTAYMEQEAFKRDIQKAGEEALSYIRANGLKGI
                     VLAGRPYHIDPQINHGIPEMINSLGMAVLTEDSVAHLGRVERPLRVVDQWAYHSRLYA
                     AASFTAKQEDVELVQLNSFGCGLDAVTTDQVQEILNENSKIYTTLKIDEGNNLGAARI
                     RLRSLKATILERHKNGFELKKVDNRYKRIEFTEDMRTKHTILVPQMSPIHFQFLEKAF
                     RLSGYNMVVLASMDKGAVDEGLKYVNNDACYPAIIVIGQLIEALKSGKYDLNNTSVMI
                     SQTGGGCRATNYIGFLRKALKDAGFEKVPVISLNAVGMEKNSGFKFTTELLNRAMMGL
                     VYGDLLMNVLYRVRPYERIKGSANMLYEKWAEICKESVEYASRKVFKQIIRQIVKEFD
                     ELEMINQAKPKVGLVGEILVKFHPTANNNVVNIVEAEGAEAVVPGLTDFLLYSAYGLE
                     YRYKYLSGSKKSQLFGRAAVMIIEFYRKAYREALSASKRFYAPQTIEEIAEGASSVVS
                     LGHQTGEGWLLTGEMVELINNGVRNIICMQPFACLPNHVTGKGMMKELKRVYPGTNIV
                     AIDYDPGASEVNQLNRIKLMISAAFENMNKFEAHKVGSYVTTDYRRMRSTLIMRTRL"
     gene            complement(39284..40003)
                     /locus_tag="PKU88_01250"
     CDS             complement(39284..40003)
                     /locus_tag="PKU88_01250"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPX67950.1"
                     /translation="MDKKPSFKPYNQGQITFLPPSLEDLIPENHIVRVVDSAIESIDT
                     KPLYEKYEGVGASSYNPVMMLKIIIYAYTQKIFSCRSIAKNCRENIMFMWLSGMNMPD
                     YKTIDRFRIERMKDIIPDIFTEVIALLHSKGYIKLERYFPDAGKDSWAWVKNPRRFRE
                     GLREKCEELLESIDEIERQDTREYGEGDLPELDAGANIDAQSIRDTTDKINEKLDMRL
                     GDRTSRWDGLKKLWRGFSRTK"
     gene            complement(40170..46340)
                     /locus_tag="PKU88_01255"
     CDS             complement(40170..46340)
                     /locus_tag="PKU88_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX67951.1"
                     /translation="MRILKRTIAFALILAFFISSTGFTQLVYAAAEENTLMVHNQFVS
                     YVVDKNTGRFGISTVEGAPRRSGDQDAPLLFEGSKPDTSFTTFRIDGEDYIFGNSYGF
                     LGIKGGMVKAPKTDQMVNTSLWTLGDLEVEQRLTLVSDSGNPDLGNVRVTYRVSNKGK
                     TEKSVGSRLLFDTKLGSNDGSPIIIPGIDKPVEHEISLEGEEVPAFWQSADADISPSV
                     VSYGLISGWENETPDRMTVAHWGGIGSTKWNYQPDSTVKFTSPFNKHNKSDSAVALYW
                     DPEVLAPGETRVYETFYGLGVFATADGHTFMANMSGPDSLELKEDKTGYIQEEFEISL
                     SLDNGLPVSVPMENVRAVLELSGGLELAPGQSKEINLGLLQRDSRANLVWKVKGVFAE
                     TYRIGQATVYLHSDSLYEPQAYGRYVILPGSSGKVPDIQYTGITPQNLYYEDSRKSFN
                     INGTGFGLMKDRSRWELKLIKEGQNPRVYNINHKQINLAGDTTIQVTLGDMLRDISDA
                     GTYTVKLEHKDFQGCSFEKGLNISTDNQYMSRKYGILAVSDEGNKTYKIKVFNNEGDL
                     KKQPDSVKNKLLLIVRGDIQQKEAGIYEAYSRTETPVEMNEVLLYNSTTPISISESNG
                     SVKLAGNGGLSVSGSITFWKWDFEINLKKGSQYTLKPPDDGDLVNKKIEIALTGAAGG
                     LQNILAGFNLKFNTAYFYKDTDGNGLIFGGSMKLSLSGGGDEDENNNNNNNDNIITNE
                     DKKDEKKDPFKIEAKVDTVAMGQKPDNTIGFKGIAAQATVGFPKDFFPPPVDIGAEAT
                     LKVDTFSDPGEVGLALDVDLKVIKVNGEIEFILKPYPFPDKLYLYVGTDVGVDIIPAI
                     PVATLYGVGGGIDNISNLVNWDSSAPPLTIMLTASAEIGRILKMDKVTLSVSWQHAEL
                     KGNIGIKGYDIIKNATLRLRWYNPIGFHASATIEAFSCIEGKVLLNIYKDSFIGMASI
                     RLFVPNGVPVVGGLTIAGAEAGIDTEKLWGKLTVIGVTMGVTYLFGEEKVEFDVGQNG
                     KLPEGLIASLDGAEGLYAINYRDEGTGEVGRMIFGTNMKLVGSSRQPERVYAKDSIYL
                     AAAGEDRRFLFMDMPVVTALDDTTYRLNVQSREAALFEIEYEGTKPNVRVYRPDGTEY
                     MLVEDDINGNMRYQTIPAKDSDSGRDEQKLWISVVGPQPGMWTIVSDKPLTAAQLYDI
                     KMAPEYTSLDAEKIGTGSVKVDWSGKYLDGAKVNLYLLEEGSSEAGRLLVQDIDAAAG
                     TYTVTLPEDVMTGSYVIRAELSKEDYGYTSRTTGPFTVTDLKAPSKPRNLKVTAAGNG
                     CLKVEWEEGTEVRYPAQGYILTVLNDDGSSVQGYPESYVSGRTEAIIGGEVTQQDGTT
                     LRLETGKSYKISAIAHREEDLVEGEDIPRQHYSEAILSDTVFLPAPHPPALKLSLARE
                     GSMLPLNTAESGIDEYYSTGKEAMLSVEADTAVGANIVLNKETVYSFNPAVRHLQQLA
                     LNEGENMVEIRAENSSGDFTDKVIRIICDTIPPLLLINSTEVTQSNGAASALIKGKCE
                     PGSKLTINGGNVLSDENGLFEYTLDMGDNMSMEIFAVAEDPIGNISEYSGTVYNDRLK
                     DIQRVAITPGGATVELGESLELSLNAIDSEGKHLSIKPELVKWSLMADTGTAVLENGA
                     RIKALKPGKAYIMAEYKVTEDYSHTDAVVVNVVESMGNKPITASRRSSSPAGVLERII
                     EAEKGKKDIYTARLQPGTETKLTADDDLTLDIPAGFAVNSADINISRYDNQEELMKKF
                     PGMKLLSPVYDISLNKGASTGTPVSVSFRYEADQAADLRRIAVYRLDEQQGGWDYIGG
                     TPDKEKGIITVRLSSFSKYAVLENSSLRLLDDVDDARWSRDVIYSLVHMNIVEGIKVG
                     EKYYYMPGNYITRAEFIKLLSAGLEAGDTEADSVQLPFADKKEIPDWALSHIKAAYAN
                     GWVNGRIAGDKRLFGPKEQITREEACAILGRMLGDGIKPKRVYFTDRNQVAKYAINYL
                     DILADMGVLSGYGDDTFRPKNFITREEAAAMIDKYLKNAS"
     gene            complement(46340..52114)
                     /locus_tag="PKU88_01260"
     CDS             complement(46340..52114)
                     /locus_tag="PKU88_01260"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HPX67952.1"
                     /translation="MRNLTVKGMISVIMALVLLTSQIVPVAGSGTDVRFDNGAHFASK
                     GIKNCYPDKGQWSTNETNSFYIESHPETGDEIKPKNINNSFKYFWTMPLKGADNQAGL
                     KYKVRLQAYGQAFDGDVASIFLDFWAPLGDGRSGSITVERMQHDFSGWNDVSFSGYVP
                     EGATGMQLTLAGEKGISILDDNKCEIYFRNIEVYITDDVAPIPKYVEFGERRELGTPE
                     ERRMKDYYGIGDDVYWDLEFNEPIFINDPGYLAYVRGLNATERNSFLNAQLSPNYPDE
                     KLLLEHVRDNNGRGILQQFGELKMKFKYRNADGRDMEGYAYPVDKSRYNKETYGNDYS
                     KQIKFKYTVRPGDEFRASDIYEMVLEGGVITDNAFNPMPNEYRIISFASGSASGVSNV
                     YRIYTRDFRVETTAPVLLEINGGLPEGTLDRSQTLPLYFKFSEPVYITISDGAFIKHN
                     INKLEFENYQRAGRFSSYDAKLYLNALNRDWTGALDPYRSGTPDAYYASGNGTTMLEF
                     RFDNMENSFDPLEITGSIYSKWKESGSTERVSYGELYVMDAAGNKAPLLPIGGVKLSD
                     SKRYVGDTEPPVITVHTKEAEKEKGGFYVKINVTDEVSGVDYDTLKFGIGYTNADFGG
                     IMRAAKSLAPGRWYHSHELAGMFGIDPGRTSNYRLFVHAKDKKGNMFYGSTHGYIGYG
                     WATDINVTRTDIDISSIETQKEMSVSLETNLGGAAEGRVYLCSADWVREDGVNWRCKG
                     LWYCMTREPVMPEFSENSGIWRYKESHRINTSEDFEGDPRERDGYYYLHIIAADEERK
                     PIGSKTVPQPLLFDFKPPQVTANAEMQADGSMKVTIHVSDNYTPRKDIEVKYILTNSD
                     RQQLPENGEIIIGKDELKPDTYVNVTATDAHQNNRNMLFGPYGNAESKLSASAPYINF
                     YRGSKYTNEDFAELHIFNSVEEFSWSMDGVSWSGWIPLEKGATSFETQGYSPCIPLPD
                     KEGPITFYTKYRDSAGKESDAAASTVIRDVTPPTARVKYSSYYGSNYFFTAELVDISD
                     NLCPSEDIKVIGYVPGYYKEIIARGEPHYFILEDEAGNKGAVKACVEPIPVVIQPVVD
                     DDDDDDEPEPEPPDTESPTIVVTPNGNHSNAMSVSPKIKVSDNKNIARIEYAFTTESN
                     IEDVTDWTEIPMDDYNPNGNTVSFSGVSGTYYLHVKATDDSGNTGEFTSNPYNMVLEY
                     TEPDIVYSGEHEGVMRAFIVSPEPITVSEAVYDLRAGEEYTFRYTYDSDGPDAEDWLI
                     TAAWESEIDPDMLECKGTVTMSPYDEVTSGPVTVTIEANDGYPMEFMPERLDCDWETL
                     EGALNQYLIDNDNINIIEGRVMQSAFPEDITIRTINSEYLNADLLEELASDEYYYLEV
                     DKAEITFDVNGYFLYMAWGNLYTVQVGHIVNEDWVDNVTVAMTEGSRRAYAPGSLLAS
                     AGKYSGMLKASDSSSVDDLTPPSGRVSYTWADPKKGPVTANLKLSDDSRGNVTIINNG
                     GSGRYVFKNNGQFVFEFEDEAGNQGRALAQLTAIPAEGPRVAVSYSSVYPTREAVKVT
                     MTPEPGSELKSGDIQTVQENDSYTFITEVNGSWNFTFVNEYGIEKVVKPSVENIDRIP
                     PKLWLDYIYDSYKNTITAIVRSDELITPAEGSTLSQVYPCTDKERYTMRALDELGNEV
                     VVTVSAAYLDEPGAGDVKINFSTAELSKNPVTITLTSDKEFTVLNNGGSKELTAQQNG
                     QYIFVIRDSLGQIKALRLEVKNIILEAPVITLGYPDYIEINRRGSIDLMNFTAVDSID
                     GDVKDKVKITGAVNTLKPGIYTVTYSVVNSVGNEAVKTLTVKVRSDEEQIVRINGVRY
                     EGEPIPLAASKLQVTAEGFVGQVSLKLAEGFRNASYFKTDGTAGSLDNIPVSKKGWLT
                     LYVYDNERNSKLINVLITDLGGEQ"
     gene            complement(52147..52383)
                     /locus_tag="PKU88_01265"
     CDS             complement(52147..52383)
                     /locus_tag="PKU88_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPX67953.1"
                     /translation="MSYIRTVLPMKDYRLFMEMEGGSTVIVDLSGKLQTAKYAELTDE
                     AFFRTAVTDGDYVLWGNGRVRLTVGELMDVILLG"
     gene            complement(52733..55204)
                     /locus_tag="PKU88_01270"
     CDS             complement(52733..55204)
                     /locus_tag="PKU88_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX67954.1"
                     /translation="MGRSRISRGELHYFSDSLKQKLKDILHALVTVVEAPSGYGKTTA
                     IRDFLKAELSQGTPVYWFTAVDEAPAAGFRRLCHEIDKIDSQAGERLLKIEFPNAVTA
                     GEACDAIRSIQCRHEAYLVIDNFQLLHTFLPPSFFTALLEHGGEGLHIIIVTQLLKRN
                     MLTAIAGRGFLHITASDLKLDAEDIRRYYALAGLRITHEDALAVEHYTEGWIIAVYLQ
                     LCAYLDTGVLYGMSGILALMEHLVWDTLTEEQQVFLLRLSPFEMITVQQACALNGFDK
                     LPEYALDALESPFIRHEAYEGRYELHSILSQLLIQKSSERGSAFERKCFLSAGDLCRD
                     EGKISSALGFYWKAEDFERMLSMDFSNNILENIGDSHFAELALDIAANCPIDIKRNNM
                     LSMLRVAWALYMNGKKAQYELLLEELRELPELSGDSDLMGEWLLLASYRSYPKIAEMT
                     AALRQAAVLFKGRCSRVILPSAPWCLGHYCTLAELHTVPGEVDREAEELEEYIGLYSQ
                     LTGGHGSGADVLFRAELAYHRGNLSEAEILAYKAAFLAESRQQSIVLLGSTIQLAEIA
                     LHKADTAGWQNAISSMERAASFTSQDSFILRQALDIARGVLFNELHQLERIADWLKKG
                     DFSDKRLTPLMISNAGFVHISYLMHRGEFPRMLGMLEATYPERLGPNPFRDMILYLTL
                     AVGYLSVGNRDQACALVARAGEMALPDGLIFPFAAYSWLLQGLSDELVRKKYPELTDK
                     FNEIKDRFSSGWTRLHEDITPEEFPPDLTPREYEVAKLAAEGLHNSEIAKRLVVTEST
                     VRTHMRAIFQKLDIDRRTKLAEKLK"
     gene            complement(55469..55810)
                     /locus_tag="PKU88_01275"
     CDS             complement(55469..55810)
                     /locus_tag="PKU88_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67955.1"
                     /translation="MEHVYDSQSRLQGYVDGDVIYDGWNRVAGYTDGRMIYDEYSNPM
                     AYVDDRFVYGMDGRPIGFYSNDYRLYDMSGRYMGYGNQGFRGLLGTVLLLLLLRRFRR
                     RRRRRRFGFGF"
     gene            56214..58871
                     /locus_tag="PKU88_01280"
     CDS             56214..58871
                     /locus_tag="PKU88_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPX67956.1"
                     /translation="MWFSKSPEEVLREFDVSQSVGLSGEEALKRLEKFGENKLKGKPK
                     KSLISLFLGQLRDMLIYILLAASVITILIGEYVDAVIILMVVILNAVIGVIQEYKAEK
                     AIEALQKMTTPKALVRRDGEVKEINSEELVPGDIVVIDAGRYIPADLRLLGSVNLQIE
                     ESALTGESVPVDKNANALFDNPKAPLGDLSNMAFMSTLSTYGRGEGVVVATAMETEIG
                     KIAKILDEDSDEMTPLQKRLDELGRILGFLCIGICALIFIVALIQKRELFEMFLTAIS
                     LAVAAIPEGLAAIVAIVLAIGVTRMSKINAIVKKLPAVETLGCVNIICSDKTGTLTQN
                     KMTVVKHYTLNNLKELPAAQTAFEAVGDEAELIKSFVLCSDATYENGKGTGDPTEIAL
                     VVMGDKYNLSKTSLNEKFERVGEKPFDSDRKLMSTLNKEGNGYRVHTKGALDNILKIS
                     ASALVNGKIVPLTDGMKADYMKVAEEMSDEALRVLGAAFKDTGCIITPEEMEKDLTVI
                     GFVGMIDPPRLEVKDSIREAKTAGITSIMITGDHKNTAVAIAKELGIAESREQSMTGS
                     EIDELSVEEFAERIGSYRVFARVSPEHKVKIVKAFKSHGNIVSMTGDGVNDAPSLKYA
                     DIGVAMGITGTDVAKGASDMILTDDNFTTIVHAIEEGRNIYNNIKKSVIFLLSCNLGE
                     VISIFASILFFWPIPLLPTQILWMNLITDTLPAIALGVDPGDKDVMKKKPRDSKESFF
                     ARGAGVKAITGGSLIGILTLAAFYFGLHEFGYSLGSGEIPEEVLTYARTMSFVVIVAS
                     QLFFSLSMRNATKSIFQIGFFTNMKLIGAIIAGFILQFGVITIPVFARAFKVHTLSLY
                     DWSLVIAFALVPFLVNELLKVFMKAVYKE"
     gene            59126..59344
                     /locus_tag="PKU88_01285"
     CDS             59126..59344
                     /locus_tag="PKU88_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67957.1"
                     /translation="MVQDSGPLIELDSMLNEFNSEKLPFVGDRSGPIGKFTNTKAVLK
                     ENVHITLPNALSTIAPGKKASCSTLMLC"
     gene            59741..59962
                     /locus_tag="PKU88_01290"
     CDS             59741..59962
                     /locus_tag="PKU88_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67958.1"
                     /translation="MQSHRIEDTGTIDFKAFILYFENTFLETIAEELFGNRNLVLKNN
                     CFELSPELRELIYIHPRMPCKAAGLFHDA"
     gene            59952..60158
                     /locus_tag="PKU88_01295"
     CDS             59952..60158
                     /locus_tag="PKU88_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX67959.1"
                     /translation="MMLDLKIEKAKDLLQSTGFSIEQICDLCGFSSLSYFSQIFKNKT
                     SLTPTQFKGQYNHSSKLFFFKGKE"
     gene            complement(60175..60615)
                     /locus_tag="PKU88_01300"
     CDS             complement(60175..60615)
                     /locus_tag="PKU88_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67960.1"
                     /translation="MRKIIDERIIAGKRKINSSAFGICFLALWGILLYRQFMLHQDIR
                     EYADIFLLTVGISIYVVVSNILQGFYYTYRSRSTQKKTLLIGALTGTIVFSTVQILIM
                     DYDLANGKDVSKLVLQAIIFLAVWIACQHALFKISEKQADKDIE"
     gene            complement(60619..60819)
                     /locus_tag="PKU88_01305"
     CDS             complement(60619..60819)
                     /locus_tag="PKU88_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX67961.1"
                     /translation="MGDKNNKLRLARIERDLTQEQLADKIGVTRQTIGLIEAGKYNPT
                     IKLCIAICKELNKTLDDIFWED"
     gene            complement(60869..61753)
                     /locus_tag="PKU88_01310"
     CDS             complement(60869..61753)
                     /locus_tag="PKU88_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX67962.1"
                     /translation="MRVLLPIAYAHRKMAGIPLKGWLSFAQAIERRFIELGGSIQYQS
                     KVERLIIENGAVKGIILADGSRHFADRVLSAVDGRFSQSLLLGKPEGDTKQTFNPNAV
                     SDQPVQVNIGVDMDFSAEEGPVTYILPDSFNAAGREHGRITVHNKCFDPDAAPKGKSA
                     ITVFLDSDYLWWKDFASDKARYKREKDRCAEAVIDIMDRYRPGFKDKIEVIDVTTPLT
                     RERYTGNWMGAMQAWKPDSSIIRALLQSRPRYAYKDIQGLYMAGQWVEAWGGITTAAQ
                     SGRKAVQAMCRHDGKCKI"
     gene            complement(61921..62859)
                     /locus_tag="PKU88_01315"
     CDS             complement(61921..62859)
                     /locus_tag="PKU88_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HPX67963.1"
                     /translation="MLSNFEYIRESLELNLFFMRIAKEHSIFIEAAFTAKNREPAEEA
                     EAFKIVFSNLLIKAIELSDGILSEDVTGSGELVTDLTLEAEKETEFYTGISINSRITK
                     AELAFANEEEPSIDPVLLQDVYMLNQQALASTRALANYKARLLRDVLACRLFTNNYPL
                     LLDHILREAKFYIRSLMRLQRKNSSNTIDDAIKEEIFWNRIMAEHSKFIRGLLDPTEV
                     KLFDTANDFGNRFDMLTAEAEKLEDNLNLFAKVTTETSDAAKDIRDFKRQGTQGLINC
                     TIRSIAYPLLGDHVVREANHYLRLLKKYASMPVSSR"
     gene            complement(63078..64169)
                     /locus_tag="PKU88_01320"
     CDS             complement(63078..64169)
                     /locus_tag="PKU88_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPX67964.1"
                     /translation="MNSKIKCGAVIAAAGMSSRMNDFKPLLPLAGTTLIGRVINTLKS
                     AGVSPIVVISGKFAERLKEYLTGFDVICLYNGNYERTDMFYSACMGFEYIQDKAEQFF
                     FLPGDVPLFSKQSLDAMMEHMSISGCDILLPQRCGRQGHPILIKSSVVPAVLSYDGEN
                     GLRGVIENYKGPKEAMELPDIGITLDADSPRDYELIKLLADDTVPEDIRRHSALVAQL
                     AVSLGEKLISLGYSLDLHRIELAALLHDISRAQPNHACAGAEHLEKIGHPLLAEIVSQ
                     HMKLLPEQEDKISEVTIVYLADKFFIGGEIATLEQRFENKRREFHDKPGALQALEENY
                     QTSLGILRLFENALGFSIVPNELLKKQTV"
     gene            complement(64171..64482)
                     /locus_tag="PKU88_01325"
     CDS             complement(64171..64482)
                     /locus_tag="PKU88_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HPX67965.1"
                     /translation="MEFKNATIVKKANIYFDGKVSSRTIFTANGERKTLGVMLPGEYE
                     FGTNAEELMEVLGGEMDVCLPGEKVFKTYKEGDSFIVPANSKFKLILKTVADYCCSYK
                     E"
     gene            complement(64524..65759)
                     /locus_tag="PKU88_01330"
     CDS             complement(64524..65759)
                     /locus_tag="PKU88_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HPX67966.1"
                     /translation="MTLISPVQQWAAERTGLKEKLSLQTLMAWQLDKVRKAVEYARNN
                     CDYYCKKLKGIDISGIKTLRDMQDIPFTYPEDINEDPKAFVCVPTREITRITSLSTSG
                     SQGSPKRIYFSENDLERTIDFFACGMSTMVHSGQSVLIMMSSRTENSIADLLEKALKR
                     IGVSSDIHGNVKDAAAALEAAKGYDCLVGVPAEIMYLCRTDDRLRPESVLLSADYVPE
                     SVIRYLEETWHCKVFTHYGMTETGFGGGVQCEAGEGYHLRDADLLIEIVDSITGKQLN
                     AGKLGEVVLTTLRNEAMPLIRYRTGDIARILTKPCPCGGILPRLDKVRGRSANIIRLE
                     NGKSINIHDLDEIMFAIPGLRNYSARLVGRNRLLLTADTKSQLDAELLREKLPCDIKV
                     EYGTVVPFTGAGKRNICLE"
     gene            complement(65756..66391)
                     /locus_tag="PKU88_01335"
     CDS             complement(65756..66391)
                     /locus_tag="PKU88_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX67967.1"
                     /translation="MKNICTGDICRPGGYKITERALEFCGFSKDAKLADIGCGLGATV
                     RHIRRNYDYDICGIEKDKDAVKLAVTQFVYAGDSTELPFDSEELDGLLFECSFSKMDN
                     PELVLTECQRVLKPGGYLIISDMYARGSSAALSGLLGRIEAKEEILDKLIMSGFDIKL
                     FEDYSDELHRMWGQLIFEHGAKALCENLGTDSGKLKEIKCGYCLIIAQKGA"
     gene            complement(66388..67698)
                     /locus_tag="PKU88_01340"
     CDS             complement(66388..67698)
                     /locus_tag="PKU88_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX67968.1"
                     /translation="MSIPKIIRSTYSVCPVCLSRIPAQHVEIGSNVYLQKKCVQHGFF
                     SSVIWRSKTPIGEWIGDTPRIKKNENLNCPKACGICPEHRQGTCCVLLEVTSRCNLRC
                     KYCFADSRNTDDPSLKQVQAWLRQLAVPGKTLVQLSGGEPTVRDDLPEIISSAKEAGC
                     RYVQLNSNGIRLAEDKEFVRKLAEAGLSFVFMQFDGTNDEINRKLRGVDLLALKKQAI
                     ENCAAYNIGVTLVPTMVPGVNTDNIGELLRFAIAESPAVRGVHFQPVSYFGRIPERPV
                     DEVRYTLDELIYDIEVQTKGQIKAKNLIPSRCDHPLCGFHGDFVVMPHGIEPLSGKYT
                     SKGDCCGSEPVSADKNRAFVARRWERQVQEQESHSCCSKPDIHDMDYFLKRVKSHGFT
                     ITAMAFQDAGNMDLERLRRCSLHVFDNGRFVPFCAYYLTGWDMT"
     gene            complement(67699..67893)
                     /locus_tag="PKU88_01345"
     CDS             complement(67699..67893)
                     /locus_tag="PKU88_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67969.1"
                     /translation="MADNKVIICCKCKKELIPKKVYFKYLGHSFFTDILKCPDCGEVF
                     IPEELVKGRMAEVEMQLEDK"
     gene            complement(67897..70146)
                     /locus_tag="PKU88_01350"
     CDS             complement(67897..70146)
                     /locus_tag="PKU88_01350"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX67970.1"
                     /translation="MVLEMEKFKEKIKTCLQNEPAFCTAACPFRLDIHDFMPKMQRGG
                     FNAAYRTYLNTVGFPGIVSELCHEPCKNVCPRRLKDEAIAMKHLEKAAIKYARDLNPN
                     SYNLPPKNKKIAIIGAGISGLGCALRLASKKYNVTVFERTDRIGGHLWDVLPPEVFLA
                     DIERQFMHEKYTLNLNTEITSLDELDFDAIYVATGKGGTDFGLKPDPEGAFASDKPGV
                     FMGGELCGRNIIEALADGLQAINAIERYIKTGNMNQPKAKDKTEILINTDLISYMKPV
                     LPDEGEVFTQENALYEAKRCLRCSCDACVRHCDLMSYYGKYPKRIGEEVEITVNPGTL
                     DGNGTVATRLISTCNQCGLCKGICPQEIDTGDFLLQSHRAMRGKGAMPWAFHDFWLRD
                     MEFTNSEAAGMCKLPKGFDKSRYVFFPGCQLGASDPWYVIESYRFLREKTPDTALILG
                     CCGAPAEWAGDEQLHEAAVAKLREDWISLGKPIAVFACPTCRQMFRKYLPEIEGIFLY
                     NLISELGIKPSPRVNGDTVSVFDPCTTREEPELQHVIREIAEKAGFNLEPLPYEGKYA
                     KCCSWGGQVSIANPSFTREVVKARISQNSNPYITYCINCRDIFDAAKKPVYHILDIIF
                     GSHDKDQMLPTITQRRSNRINLKHKLLSEFWRDEAIMEQKESRIKLLITPELRQKLHK
                     EMILETDIETVIEHCESTGRKVQIPESGHFFGHLQIGNMTYWVEYLPEDGKFNLINAY
                     CHRMSIEEA"
     gene            complement(70258..73179)
                     /locus_tag="PKU88_01355"
     CDS             complement(70258..73179)
                     /locus_tag="PKU88_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPX67971.1"
                     /translation="MKLRKMWLNVNGANRMFACNPEKDTLASVLRRMGLTGVKVGCGT
                     GVCGACSVILNGKVIRSCTKKISTVEEYSKVITIEGIGTPNNLHPLQVAWMNYGAVQC
                     GFCTPGFIVSAYALLQENNNPTREEVRDWFQKHRNVCRCTGYKQLVDAVMAAAKVVRG
                     EATIEDISFKLPEDKEYYGKPVVRPTALAKVCGLADYGNDQELKMPAETLHVAATQPR
                     VAHHAKILNIDTSEAEKMPGVVKIITHKELKEAGGTNVMAEAQFHERTTVMVPSRKVL
                     CEDKIFRYGDVVALAVADTKEHARAAAAKVKVEIEKLPEYLSYLDAVMPDAMRVHEDT
                     PNIFSMQPVLKGVGLEDAAKVQEVIDNSAYSVSGSFYSTREPHLTIEGTTVQAYFDEE
                     DNLTIQCKSQGVYSSKGRMGDSVGIPKDKLRIVMNPTGASFGWSTNAGDICLAAAAAV
                     VTKMPVALSMSYEEHQHFSGKRCPCHSNGRAACDENGKITAVEFDFGMDHGAYSWGGD
                     DLMTKQVRFAFFPYKVPNAAGLTRIANTNHNFGTAYRAYGSPQAYTLSEAMMDMLAKK
                     AGIDPFEFRWINIAREGDTNINSYPFRQYPMEEIMKIMRPYYEKAVAEAKAADTPQKR
                     RGVGLSWGGFNVTEGGTDSSSVALELNPDNTFTKYDTYQELGQGGDIGSLMLTLEALK
                     PLGVKPDQIRLVQNDTKLCPDSGMSGASRTHFMSGKATILAAEKLMNAMRKPDGTYRT
                     YDEMAAEGIPTKYQATYSNTTIPGICRLDPNTGIGDPTPAYTYCLNLAEVEVDTATGK
                     VRVLRFTCVDDVGKIGNIDAVNGQAYGGISHCIGFALSENYDDVEKHSNMLGAGVPYI
                     KDIPDEINVIHYERPDEVGPFGSSGASEAFQSSGHVAVLNAIDNACGVRIYEMPATPE
                     KVKAGLDILAAGGKIEPPKKYFLGSDLYDELENIKANPVKYGGDDFFIPLGDATAERF
                     F"
     gene            73773..74213
                     /locus_tag="PKU88_01360"
     CDS             73773..74213
                     /locus_tag="PKU88_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPX67972.1"
                     /translation="MENIMTLEKVNELRSQGFNCSQIVFLYGSERLGLEPEKALKIAG
                     AFGGGMYNGETCGCVTGALMVLGLKYGQSVPYDLAAKNLLIQKKTEFEEKFKEAHETL
                     ICKEILGYDISNPSEKSIIVEKGLLDEICPYLIWDACQILDEML"
     gene            complement(74342..75244)
                     /locus_tag="PKU88_01365"
     CDS             complement(74342..75244)
                     /locus_tag="PKU88_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX67973.1"
                     /translation="MNRIKLVLVMIIWGSLGVFTKSIPLSALSLAFLRASIALPVLFV
                     VMKMKKADKLKWTLLKPYLISGILLGFGWLTLFYGFKNTSIASAVIIYNMSPVYVMIF
                     APLALKETISKIQVAVILTSFLGLFLIVGHNLLEGYGYMGMLLSAVSGMIYASIVLIN
                     RSIKVRVDNQTATFVQIFTAMLVLLPFVLIEGNLLTAVHLDAKAAIYTILLGVLHTGV
                     AYTMFFSLYTNMKSVEIISYSYLEPLFGILFSVIFMGETLIFTQIIGGILILGSTFIG
                     EMLKNRGYLEKKIHSNLSANVTDR"
     gene            complement(75316..76095)
                     /locus_tag="PKU88_01370"
     CDS             complement(75316..76095)
                     /locus_tag="PKU88_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX67974.1"
                     /translation="MSDKSRMEFSRSIIEGVEIKLCRNDPHAYKPHVHNELSLGYIVE
                     GTTDLTLNGSTIHYGPGDGVIIPPLMTHRCAPNDINHWAYVMLFVDPGYYGDLVTFNQ
                     AKKLTGNQLQRLVGFIEQLLTEKIPNTLENILIELLIEFGEDDIPETAAANTIDNIKK
                     IHDYILNHVSDDITLDKLQQISGLNKFSIIRNFKKLYLTTPAAYHLQYRVAEAKRLLS
                     KGADVFEICVELKFYDQAHLIREFKKMYGVTPGAYIKQLMG"
     gene            complement(76343..79774)
                     /locus_tag="PKU88_01375"
     CDS             complement(76343..79774)
                     /locus_tag="PKU88_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HPX67975.1"
                     /translation="MLTDNAVKVLEKRYLIKDENGKPVETPEHMFRRVASTIAAADSF
                     YGSPADVAETEEKFYKLMSSLEFIPNSPTLMNAGRELGQLSACFVLPVEDSMEGIFDA
                     IKNAAMIHKSGGGTGFSFSRLRKKGSAVATTGGVASGPVSFMKVFNAATEAVKQGGTR
                     RGANMGILRVDHPDIIEFIQCKKDNSDITNFNISVAITEKFMEAAEKGEDYELIDPAT
                     GKPAGKLNAKEVYDLIVDMAWNNGEPGIIFLDRINRDNAVPRLGEIESTNPCITGDSW
                     VLTRKGPVQVKDILGRQVQLALNGSYSMSDEKGFFSTGKKSIIRVCTDRGYELKLTKD
                     HLIRTAANMTRFKIDEEWKAAGDLRPGDRIIMSNNRSIRWEGKGSFDEGYLLGVLIGD
                     GTLKKEGGIISVWGSDEEAVSLMKAAGEAACALPHRSDFRGFQTVISDREENRMRLSA
                     LRDLALEYGVMPGDKKIASEVEKGSYEFYRGFLRGMFDTDGTVAGTQEKGLSVRLWQN
                     DLDTLKAVQRMLGRMGIISAIYSNRKEPGIKSMPDGKGGLKDYNVKAGHELVISKDNL
                     YVFHELIGFSSSKKQNRLKSGLGNYKRALNRERFVASVTEIIESGEAEVFDVQVPGIN
                     AFDANGIYIHNCGEQPLLPYESCNLGSINLMSCIKKKNKFGKVIDYEKLGSIVDESVH
                     FLDNVIDVNKYPLPMVDEMTKGTRKIGLGVMGFADMLVELGIPYNSEEAVNTAESIMK
                     FINERSKAASMKLAEKRGTFKYYEDSVFKEKGIRIRNATTTTIAPTGTISIIAGVSSG
                     IEPLFALSFVRNVMDNDELPEVNPLFERVARERGFYSTELMKKIAAHGTIQDIKSIPK
                     DIRKVFVTAHDIEPEWHIRIQSAFQKYTDNAVSKTVNFSNSAARDDVSAVYMLAYRMG
                     CKGVTIYRDGSRESQVLNIGSANKDNKEKPAPANEVLVPRTRPEVTTGVTEKVKIGCG
                     NLYITVNSDENGICEVFTNLGRAGGCPSQSEATSRLISIALRAGVDIGEIIEQLRGIR
                     CHSTLRQKGLKVLSCPDAIGRALERYMNTKVDNKDIKTMDELAMEIEERGNCINNCSL
                     CAEPCHAVTVEEGSISYNAYGSFNGNMKNCPECGSTIQHEGGCVICRNCGYSKCN"
     gene            complement(80033..81202)
                     /locus_tag="PKU88_01380"
     CDS             complement(80033..81202)
                     /locus_tag="PKU88_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPX67976.1"
                     /translation="MMRKWTIQAAVILLGIMFQMNAANAERYIYGKNEIDLSYEKDCY
                     LQQEGPDWELMKKRLKVKGIYMTGYTVAMTERFNKLIDLVKTTELNAVVIDVKDDNGL
                     MTYSSDLPDVDFTGANKKVRIKDIDAVMKILRGNNIYSIARIVTFKDRIAGDKYANLA
                     VKNTSGGIWRDRHGMSWLNPYNRESWDYLVDIAEEAAAKGFNEIQFDYVRFPTDGNTK
                     IIDYGTSAAGKSKAEAIAEFLSYGRERLSKMGVVVSADVFGLVTTATDDMRIGQHLES
                     IAKSVDVICPMVYPSHYGKGSYGVAEPDFEPYKIVNRSMSVAQERINAMKDVRRKAVL
                     RPWLQDFTASYLDRYKRYGPAEVRAQIDATYDAGQEEWLLWNAGNVFTSGALLKE"
     gene            complement(81387..83048)
                     /gene="ilvD"
                     /locus_tag="PKU88_01385"
     CDS             complement(81387..83048)
                     /gene="ilvD"
                     /locus_tag="PKU88_01385"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPX67977.1"
                     /translation="MRSDQMKAGIERIPHRALLKGLGLTDEEIRRPMIGIVNSQNDVI
                     PGHLHLDDIMEAVKAGVRMAGGTPFQFPAIGVCDGLAMGHEGMKYSLISREHIADSVE
                     IMAMAHPFDALVFIPNCDKIVPGMIMAALRLNIPSVFVSGGPMMPAFLDGKKLTLSST
                     FEAVGAAGANKMNEQEILDYENYSCPGCGSCAGMFTANSMNCMTEAIGLGLPGNGTIP
                     AVNAARVRLAKLAGMKVMEMLEKNIRPRDIVTADSLHNALTADMALGCSSNTVLHLPA
                     IAHEAGLKIDLKDINDISKATPHLVKLAPAGPNAMSDLWQAGGVQAVLKELEGMGLLK
                     TDCITVTGCTAAENLKDVTVKDREIIRTKETAYSQDGGLAILWGNLAPDGAVVKKGAV
                     LPEMMVHRGPAKVYNSEEDCMAAIMGGKVVPGDVVVVRYEGPKGGPGMREMLSPTSSI
                     AGMGLDSSVALITDGRFSGASRGASIGHISPEAAAGGLIGIVEDGDIISIDIPNNKLE
                     LLVDEKTIAERKTKFVPFEKPVPNGYLKRYRRMVTSANTGAVFED"
     gene            83354..84043
                     /locus_tag="PKU88_01390"
     CDS             83354..84043
                     /locus_tag="PKU88_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPX67978.1"
                     /translation="MANAFIPISQNIANEISDMIFLQKKYKPNDKLPNEHQLAKELGV
                     SRTTIREAVKILVANGVLTIERGRGTFVTERPDSQNDPFGISYIEDKKKLVHNWFEMR
                     LIIEPANVRMVVERASDEEIQEIIAYEKEAAELIASGKSFSAADQQFHAAIAKATHNS
                     VIELMLPAIELAIRDAISTAEYVGVHERAIENALINHRNIAHFIEQRDADGAALAMYY
                     HIKRGMTDLDF"
     gene            complement(84130..84205)
                     /locus_tag="PKU88_01395"
     tRNA            complement(84130..84205)
                     /locus_tag="PKU88_01395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84170..84172),aa:Asn,seq:gtt)
BASE COUNT        21694 a        21210 c        16499 g        24867 t
ORIGIN      
        1 tttatgcagc ataaaagaat aatgcgacgg ggacgcaggg acacggggac gtttcccctg
       61 tcccacttcc agtgggacac gcgaaacgtc cccgtgtccc cgtgtcccca tgtccccgtc
      121 gcattattct tctgatgcaa aaagggattt cgcaaactat atattcgata gtattgcgaa
      181 accccttttt tgatacaatt tattttctgt tttttgttcc cttgggcttt cctatggact
      241 gcaagtgctg agccttgttt ttctgttctt ctgaactcac ccaataattg taggtagaca
      301 tgaattctcc gtacaagctg atatcgttaa gtttgtcttc aagttccttg ttattatcat
      361 tttgatccat gttactcctc cttatggtag tttataactt ttttctggct atgatgtagt
      421 agtccctgcc cccatgtccg cctttggtag accactcaaa gttgtttttt tccagtactg
      481 aaaaaatatt ttgagcattg tatgaatcct gactgttcat tgataccacc aactgttcat
      541 ttgaatccat atgcttgaca gtatcaacaa ttcttctcac atcgttcagc gtaacatttc
      601 ctccaacact tatcatcctc tcaaccattc aactgcctcc tattattgag tgcttttcac
      661 aaaaatccat gaaattgctc atttatagtt tatgaaactg ttttttaaat atacacaaca
      721 aaaaaaactg tctcacgacg ttttttttcg ctagggaacc ctatggttcc cttcgacgga
      781 tgcagaatgc atcctgagca ccctccttcc acggcaggtg gattctcccc cttgcatccc
      841 cccattttat aggaaagtat cctttcctat aaaataaaaa aaagcaataa aaaacatggc
      901 aagtttttaa tttgccatgt tgtttctgtt ccctattcct tttctctttc tgcattagcc
      961 gcttcgataa tcttctgggc tatctgtgca ggtacttcct catatctttc aaattccatc
     1021 gtaaagcttc ctcttgcctg ggtcatagat cttaagtcgg tagcatattt gaacatctca
     1081 gccaaaggtg cttccgcaga aaccttctgc atctttccgc agggttccat tcctaaaatc
     1141 ctgcctctct ttttgttcaa atctccgatt atatctccca tgtattcatc cggaaccagc
     1201 acttccacat gatatattgg ctcaagcaat acggagcttg cagctgccat acccttctta
     1261 tatgccagag atgccgcaat cttaaaaggc atttccgccg agtcaacagg gtggaaggat
     1321 ccgtcataaa gagtgcatct gagattaaca accggataac ctgccagcac accttcttcg
     1381 atgcattccc tgagtccctt ctcaactgca ggtatatact gtcttggaac tacgcctcca
     1441 actatcttgt caacaaattc aaaatcagcg cttccgtcaa gtatcggctc aaactcaacc
     1501 caaacatggc cgtactgtcc atgtccgccg gactgcttct tatgcttccc ttcagcctta
     1561 gccgattttc ttatggtttc tctgaaaggt atcttgggat ccttaagaac cacttccacg
     1621 ccaaacttat tctgcagctt ctttgttaca acttcaagat gctgctcgcc cattcccgaa
     1681 atcaaagtct gttttgtttc ggtgttcttc tccactttta acgtaggatc ttcttcaaca
     1741 agcctttgca ggccgtttcc tatcttatct tcgtcccctt ttgccttggg ctcaatagcc
     1801 attgaaatac ttggagccgg gaattgtatg ggttcaaata ctaccggatt attggcatca
     1861 cacagggtat ctcctgtagt agtaaactgt aattttgcaa ctgcagctat atctcctgcc
     1921 acaagcttat ctgcagccac ctgcttctta cctcttaaaa ggaacaggct gctgagcttt
     1981 tccttctttt ccttgctgct gttcaatact tccatatctg cagtcaattt tcccgacatt
     2041 accttgaaca ttgaaatctt tccgacaaag gggtcggcaa ctgttttgta tacctgtgcg
     2101 ctgaaaggag catttacatc agcgggaatc ttaaccttct cattgctctt tgggttgact
     2161 gcttcaggag cctgtgcatc agccggtgac ggcatataat tcattacagc gtccatgagc
     2221 acatctattc ctatattctt tattgcagcg ccgcatagta ccggcgctac atccccggct
     2281 acaactccaa gcctcagccc tcttattatc tcttctcttg aaagctcttc gccgccgaag
     2341 tatttgtcca taagttcttc atcagtctgg gccgctgctt ctattatcgc attcctgtat
     2401 tcttcggctt cagccttgag ttctgcggga atatcccctt caacagtttt attgccctct
     2461 ctgatcttgg ccttcatatc tataatatcg actattccct tgaagcttgc ttctgatcct
     2521 atcggaagag caaaaggaac tattttattt ccaaaaactc ccctaagctg ttcaacaacc
     2581 ttcgagaagc ttgcattttc tctgtcaagc ttgtttacga agaacacaca tgccttcttg
     2641 ccttcgttaa caaaatccca agccttctcc gcacctactt caactcctcc gatgctgtca
     2701 agagctatta ccgctccgtc aacaaccttc aggcttgcga ctgcttcacc tacaaagtcg
     2761 aaatatccgg gagtatcaat tgcattgatt ctgcattcct tccagtcaca tggagctgtt
     2821 gccgcactaa tggaaatttt tcttttgatt tcttccgggt caaagtctgt aacagtattt
     2881 ccatcatcaa cctttccgaa cctatccaga actccggtat taaacagcat agcttctgct
     2941 attgttgtct ttccggcgct gccgtggcca aataaactaa tattccttag gttttccgtt
     3001 ttgtaatctt tcataaaaat tccccctttt tatgtttatt ctgtccgtca ttagcaattt
     3061 taaacgtgca aatactttat taacataccc tgcttcctgc tgttcaatgt atcagcacac
     3121 tttccaatgt gtgccctgag gatatatgtg cactgtaact ctaattttat ctttccgtga
     3181 aataaatgta aagaggaaaa taacaaatga ctggtttcaa acctctatcc tttcaaaaag
     3241 ccttacccct atcgttgaga gtatattgta cagcagcagg tccaatatcc caaagaccag
     3301 caccagtgta agcaatgtca tccacagggt aaagtgcaat gctataattg taaaaatcgg
     3361 tatacccgcg actaatacac tgacaagcat attgagcaca acattaaaat tttgctttac
     3421 cgccttctgt tcattatccc atataagctt tggaaagttc aggtctataa gtattcccgc
     3481 aaaggagctg aaagctattc caaaaatact tacaacaaca ataagccgca tgaggtaggc
     3541 cggaaccgtt ataaatactg aagctgtcaa cagcattgaa agtgtgccaa ccatacctat
     3601 gctcataccg gatagcacct ttgccgttat ctgagtctta tagcttacag gcagatactt
     3661 gcacacaaat atgttagtgc cctccctgga tattgcggaa gctgcaattc catttgttga
     3721 agtgacaaaa acacttatgc cgaaggccgc cgccaaagct atggcactcc ctctttcacc
     3781 acttaaatat atgcttaatc cttgaagctc cttcattgca tcaggctgca caataaaagg
     3841 cagtataata aataacggaa aaattaaact cgtcagcaca cagttcataa aatatacagg
     3901 agtcctgaaa agcagcttaa gctcctttat ggtatatgcc ctcagaaccg gcttctgcct
     3961 gccgtacctt tcaagctcgg tttctgaaaa cttcttcctt tttacggaag cctccgatat
     4021 gcccaccaca cctttgtaat acactccttc tccaaagctc aggaacaaaa tcacaaaaag
     4081 catacaaata agcataaaca gagccaggtt cacaaagccc ttcagtactt cactgttcag
     4141 cagcgccagc accgcaaacc tattgctgaa aaaaataccc gatatgattt ccgcatagga
     4201 attgttgccc tgttccagca gtttctgaag ctccagggcg ctcgtggtgg aactggtgag
     4261 ttttgttaca aaagcgttta tccctacagc caaaaacatg gctgttatcc cgcccgctat
     4321 cctgaacctg tctttatttt tggcaaggtt ggtaaacccc attaccagca tgcttattgc
     4381 agaggcatat acaaggggca gcaccggcaa cgcaagaaac agaagggtac cgtataaata
     4441 gtataacaac cccgccttgc ttgcaattcc gtagcctatg agaataggcc ccaggaatat
     4501 cgcctctgtc aggtattcat aaagcatagc aactgtaaac ttggccgcca gtatttccga
     4561 cggctttaaa ggcagcggca gcaggctttc gatgtccttg gaaaaataaa aaacactcat
     4621 aacgtagaat atgccgaaaa agaatattgt caggctggat atggaaaagc ctgttacaag
     4681 gatcagaccc tcctgccgga tctcaaaaag cacttcatat cctaccatgg ccagactgcc
     4741 gatacctaat atcatgggta aaaacgcggc aaatatcagc agggatagca atattcccct
     4801 tatgggattt ttgccggatt tccccggcct caaattcagc aaattgaaat ttttcagcat
     4861 tactccggta agtaccatat atctattcat tttccgtcag ctccaggaat attttttcaa
     4921 gagacccgtt gcttctgaag ttttcccgca tctcctccat agtaccgtag aacagcagct
     4981 tgcctttatt gataatggcc accctgtcgc acagcttctc tgccacctca aggacgtggg
     5041 tggagaagaa caccgtttta ccggcggctg cttgggtttt catcatctcc ttgagagtga
     5101 aggaggactt ggggtcgagt cccgtcatcg gctcatccaa tatccacacc gaaggatcat
     5161 ggataagcac tcccataatg actatcttct gtctcattcc atgagaatag ctctgtatct
     5221 gatccgtaag ggccgcttcc atttcaaagc tttcagcaag ccccttaatc ctttcccttc
     5281 tcagttcagc ggggacatta taaatgtcag ccatgaaatt caggtactcc atacccttca
     5341 agcgaaggaa catatcgggg ttatcaggga cataaccgaa ctgccttttt gcctcaagag
     5401 gattctccct gatatttttc ccattaatgg ttatgcttcc gctgtcggca ttcagtactc
     5461 ccgttataat ttttatggta gttgtctttc ccgccccatt ggggcccaga aatcctgtga
     5521 tctgaccttc cgggatggtg agagtcagat catccactgc ctttacaata ccgctgtatg
     5581 acttgtttac attaatcagc tctatcatgt attctcctcc cggctggcta tttttgccat
     5641 ttaattataa catatatgtc agaaaaataa agccttctaa ataatatttc actatcggaa
     5701 ggctttcata cactttagtg ttccttccat acataccctg gacttacagt attctacagc
     5761 atactattta caaagggaag ctattatctt atgatattga tataatcatt tattaacatt
     5821 atttacttgg aggaatgaat taatgaaaaa tgctgttaat atcgaatgga ataagctggg
     5881 gttttcctac ataaaaacgg actaccgcta tatttcatac tggaaggaag gctcctggga
     5941 caatgggaag cttacttccg acaacactct cacaatcagc gagggttccc cctgcctgca
     6001 ttacggccag cagtgctttg aaggtttgaa ggcttacacc acaaaggaag gcaaaataca
     6061 gcttttcagg cccgaccaga atgcaaagcg tatgataaag agctgtaaac gactcttcat
     6121 gccggaagta cccgaagaaa aattcattga tgcttgtata caggtagtca aagccaatga
     6181 agcctgggtt cctccttacg gaacgggggc tacactttac ctcaggcctc tggtcattgg
     6241 tatcggagac aatatcggtg tgagacccgc cactgaatat atatttacag tattctgcat
     6301 gccggtaggc ccatatttta aaggcggatt gacacctttg gatttctcgg tttcggacta
     6361 tgacagggca gcgcctcagg gaacaggtgc ggccaaggtg ggaggcaatt atgcggcaag
     6421 tctgcttcct catgaacagg caagggaaga gggttatgcc gactgcatat atcttgatcc
     6481 ggcgacccat acaaagattg aggaagtagg cgctgcgaat ttcttcggca taacaaaaga
     6541 caacaggttt gtcactccca tatctgattc cattttgccc agtataacaa aatattccct
     6601 tctgcatgtg gcaaaagaat atttggagct tgaaacggaa gaaagagaca tatatctcga
     6661 caagcttgat gagcttgctg aagcaggggc atgcggaacg gccgcagtta tttcgcccat
     6721 aggaagcatt acctacaagg acaaaaaaca tgtattccac agcctgactg aggtagggcc
     6781 tgtaacaaag aagctatatg atacgcttgt cggaatccaa ttcggagatg taaaggctcc
     6841 cgaaggctgg atagtggaag taaagtaaca gaaagaggca tatcccataa tgatatgcct
     6901 cttatgcaat cctgtcatgc ccccagcaaa ctttcatcaa aagcgaataa tgcctcgttt
     6961 atctcaaaga tattataatt tccttctctt atgaaatact ccacaatcag gtcaaagcgg
     7021 ctgccgggag atagggcata tcctgcccgg cggagcaaat cggcggttcg gtcaaggtcc
     7081 agctccaatg caacagccag ggctaatgcg gtgttcttgc cgggcttata cttcagattg
     7141 ctgcttatct ttaaaaactg ctttcggtca aggttggcgc gtttatatac ctccggatcg
     7201 gctttaccgc tttcatcgat aagccgaagc agcatttcgg taaagctctc gccgatacgg
     7261 tttcccacat cgtccggatt tatgcgtgaa gtatgcttct ccgcatactt ctcatctata
     7321 tatgccttaa ttggcttaaa taatttatca cttattttat atgccgccct gtcaaatatg
     7381 acaatataaa ccatcatatc gtacttcagg agaaagcttc cgatggccga tacagccact
     7441 ctcagagctt cgtccttggg gtatccgaaa ataccggaag atatgagagg gaaggcaacg
     7501 gaaccgagcc catgcttcaa agcaagctcc agagaatttg tataacatga ggaaagaagt
     7561 ttttcctcgt tataaccgcc gccttgccat accgggccga cggtatgaat aatatgcttc
     7621 gcagggagcg cataaccgtc ggtaataacc gcgcttccaa ccgggcaata gccgattcgg
     7681 ttacattcat cccgaagctt gtccgctccg gctgcggcaa aaattgcgcc gcaggcacca
     7741 cccccttgct gcaggcggct gttggctgca tttacaatag cgtcaacacg cattttagtt
     7801 atatcgttgc agataatttc cagtggcatt gattacactc ccttcagaat attattactc
     7861 aaaaacctcc acaagacttc cgaacagttc actgttctcc ctgattttta tttcactccc
     7921 caatacgcaa tagctccgtc tctttataag ttcaaacatc atatcggaca gttccctgat
     7981 atcctgctgc cttgtgccga gaatttcctc cctctccttc tgcaaatcct ctgatgtgat
     8041 tttcctcatg taattctcaa tggccctttc ccccttcata tacggggtta acggcaagtc
     8101 cagcttgctg attgttccga taatatattt tgtcatttct ctttcttcaa tctgaaaatt
     8161 cctcatatag tcatacattt catcgtatac ctttaaggtt tccttcaggt ttggatccct
     8221 gtacgaaacc ataaacatat tgccgttctt ttcaaagcct gaaaaagcgc cgtaagctcc
     8281 cccctgaacc ctgatacggt tccataggta gctgtatctg gatattgtct ttaatacctg
     8341 cagtttcccc gtatagggat aacccagttt gatgaagtta taaccctgag ctacgtactg
     8401 aaccttaccc tgggtcatca gcccttcgtt aagggactca actttaaaag cgtatttaaa
     8461 cgccggatgc ttctcattgc ttaaatcctt aagcaccaaa cccaggcccc cggcaaacct
     8521 gttgtaatca tcctcttcac aggtcactcc tgcaataagg ccttcttttc tgaatatcag
     8581 tctcttcagc ttctgaaggt tttctttcac cttttctatt ctgttttcaa aattcctttc
     8641 tatatcggcg atgaacctgt aataagtcag cccggaaata gcctccagat agctcccttc
     8701 ctgggagaag tacgagaaca gccttttgca ggcaattgtg tatccctcat tggatatcct
     8761 catttcgatt ctggacttgt tctcttgtat tatttccttg atccttctgg aatcatcgaa
     8821 tttagacctt gcaacaatct cacttatgag ttcaaacatc ttttccgtct tgcttgcaag
     8881 agccctgccc cttattgaaa atacagggtg gtatttgctg tcatcatcct tttcgccgta
     8941 gacctgtgtg ctgaacctga tgcctccggt atgcatgtca atttcctttg aaagctcccc
     9001 atagctgtag ttttcagtgc ttattctccc cagtatccct gacagcaaag gaatatacgg
     9061 cagcagctcc tggggcaccg ccgttgtatc aaataaaagg ccggcatata caatgtcatt
     9121 ggtaaacatg tggtgtgcaa gcaccttaac ccccgcttcc tccttaacct gctgaggaag
     9181 cacctcgccc ttcctgtcta tgtcctctat tgaaagcatc ggtataagcg caagctgctc
     9241 gggtgtatca ggagtattct gccacttttt aagctctcct gtctccttca ccaactcccg
     9301 aagctgctct tcgctcattt ttgccttgat gctcctgagc ttttcttttg tttctttatc
     9361 cttctcctct gacaggcctt tcttgggctt aagcaccagt agcgagctgt ggttgttttt
     9421 cagcaagtat ttttctatca gaccttcaaa atatctgtta ttgaattctg ctctaatctt
     9481 ttcaaatata tgctcataac ggagatgcat tgtggggtcc ttgtcgtaaa gccagctgtc
     9541 catgattttt atattatata tcaaaccctt gggataacct tcaaaatccg cttccctcag
     9601 ttggaactct ttcaggttga ccgccgcctc cgccaattga ctgtccagcc cattacggac
     9661 aaggccttcc aaggtatttg agaccactct tttaaaatct tccaggactt ctgtatttga
     9721 gttttttaca ataatactga aaacaggctg aagtatgctg ttgtcatatt taccaaaaac
     9781 atccctgcca agatcggctt caatcaatgc tttcttcaga ggagatgccg gagttgccag
     9841 cagtatatgc tccaatatct ccattgcaat ataaagctcg ggatcggtag acagccctac
     9901 ggcataattc aggctgagat atgtcttgtc tctttcatct tcctgggcgg atatggggta
     9961 ttccagttca tagctttgca tttcctcaaa agctttctgc accggtatgg ctgaatccac
    10021 atggattgct tcaaaaccct taaggtattc ttcatcaata aacttcaggc gtccaagcac
    10081 atccagcttg ccatacagga acatataact gtttgaagga tgatagtact ttttatggaa
    10141 ggaaataaac ccctcctgtg taagctccgg tatcacctca gggtcgcccc cggattcgta
    10201 accgtatggg ccgtccggga acaaggtctc ctggagcttt ctcgaaagca ccgattccgg
    10261 cgatgagagt gcccccttca tttcattata tacgacaccc ttgtatttca gctccccgga
    10321 agtgtcttcc agctcatagt gccagccttc ctgcttaaga atctccggat atttgtatat
    10381 gttgggatgg aatactgaat ccagataaac atccatcagg ttctcaaagt ccttgtcgtt
    10441 cctgcttgct accggataca tcgtcttgtc tccgaaggta aaagcattca gaaaagtatt
    10501 cagggagcct ttcaccagtt ctacgaaggg ctcctttgtg ggaaacttcc tggagccgca
    10561 gagcacggaa tgttccagta tatggggcag tcccgtactg tccctggggg gtgttctgaa
    10621 agtaaccgaa aacaccttgt tatcatcttc attttccata taaaagagtc tggctccgct
    10681 cttttcatgc ctaaaaagtc taactttcga atttgtttcc ttgatatcat tttcctcaac
    10741 caaccggaaa ccgtgatata tgtcaccaac tttaaattgc atccctttcc agcctccaac
    10801 aaaatatttc atcatttata ttaattataa tatatgcccg ccgtgtttta catattatta
    10861 tgtgaacact accttacata taaatgggta cgccgtgcaa aagcggttct tttccaccgg
    10921 tgcttgatcc acggtatcac ataatagtcc aggccgaaag ccctccctgc gccgccgaac
    10981 attgcaatgc cggcaaatat ataccagaat atttcagagc ctgccattgc cgataatatg
    11041 aaattcaggc acagaaatat ggagcccaga gatgcaagta tggtgaataa tcctgcaata
    11101 agtgcaaggc ctatagctgc ctctgtaagt acaatcagga cctggaacag gtaggcattc
    11161 ggctctataa aggtttccat gaaccactga aatacccggg ggggctcttt aagcagcggt
    11221 tccggataat actgtgtaac ctgagttgcg ttagttacgg catcggtaga ttccaggggg
    11281 acatcctcgg gcctcggcat tgccgcaata aagcttttcc ccgggtcaag ccaaccctca
    11341 tttattttct tcacaccttc aatgagccat ataacaccta gatataccct gagtatggcc
    11401 agccacagta taggcgtcct ggcggacaaa tgtcctccca gtatggacct gccctccctc
    11461 acataaataa attcatgcat tatataatcc caaatccttg caaatcctcc caccccatat
    11521 tgatagtgga tatttaccag gtgcttcagc gccattgcga agaatccgtc catggatctt
    11581 ccccctatgt tggctaccgc ataccggcct cctatggaca ccatgaaccc gtggtaattg
    11641 gactttaagg gctttttgcc cctcttgtca attgacgcaa gtatgttatg caccgctgtt
    11701 tccgcagtct ggagtgccgc ttctacaatc tgggggattg gcttctttcc gcctttgccg
    11761 tccggctctt catagaatgc attgtctcct acggcaaaga cattttggta atctaccgac
    11821 tgcatgtatt cattaacctg tatcctcccc ctgcctccgt gagtaagctc tgttttggcg
    11881 gcaaagtcgc tcccctgcac gccgcaggtc cagatgaggg ttctggcagg tatctccctt
    11941 ccgtccttca gtattatgga ctcctttttt acctctgtta tgggtgctga tgttattatt
    12001 tcaacatcat gcctcttcat atatctttcg gatttcttta tgagcctctc attgagtatg
    12061 gggagtatgc tgcccattgc ctccacaacc ataaggctca cttccctgct gtcaatttcg
    12121 tattccctgc aaagctcctt tctccactcc agaagctccc ccattgtctc tatccccgta
    12181 aagcctgccc ccgctacgat aaatgtaagc attgcccttc ttctgctatg gtccttttct
    12241 gtcctggctt tgtcaaacat atttttcaca tgttccctga tccttattgc gtcttcaaag
    12301 gaccacaggg taaaaccgtt ttcctttact cccggtgttc cgaaaaaagc gggttccgct
    12361 cctgtatcta taatgagata atcgtaggaa tagctttctg attcagactc aagcttccgg
    12421 cccttaaaat ctatgctctt gatttcctct gttgccacac gaacccctga tgcaccgaat
    12481 atctccctaa ggtctatctg taccgattca gcctctactc tgtttcctgc cacctcatgg
    12541 agttccgtca tcaaggtgtg atacggcttt ttgtccgcca gcagcacgct tatatccgct
    12601 ccttttcggg cagccttgcc taaaagcctt gctgcgtgaa ctcctccgta accgccgcct
    12661 attattgcta tttgctttgt atcttttccc attttatcaa gattcacttc cttatgattt
    12721 taaatacaat catatgaagt attccacaaa tacatttgca atatgaattc ttcaaaaaac
    12781 aataaaatga aacaagcaag gcacttcgaa ggaagtgcct tgctttatct gttttccggc
    12841 aatcagcatc agtcagcccg tatttgtata tatttatatg ttgctatgtc gcctgaatca
    12901 tagatgactc cgcccgagcc gtctgcgctt gtactggcac agaaagcctc agcattattg
    12961 agtacaggag ccttgttcat cgccagcgca tccttgcctg ccgctatgac ggtgctgccg
    13021 gatatgctaa ttgaacctgt ccccgcataa ataccgctgc ctgtgccggg tcctccgaaa
    13081 tcactgcacc ttgcggttac cgctgcgctg ccggtgattt cgaccccggc ggcggcgtaa
    13141 attccgtagc catcagcaga ctgcgaagtg acatataccg ttccgccggt gattgccacg
    13201 ttgtccccat agattccgta atagttgaat acgtcgtcgg ggtattcaat tccggagttg
    13261 agcgcgttga ccgtgctttc gctaatcgcc gcgtcgccgg atgcgtaaat accgctgggc
    13321 gtatttactg tactgttggt aatgtctata tccccgcttg cgtaaatacc gcagcggatg
    13381 ccatttgccg attcattact tgaagctaag accgtgctgt ctgtgataac tacgtcttcc
    13441 gcaaaaatgc cgcagctgtt cccggtagct cccgtgcttt tagctattac ggcggcgtcg
    13501 tcctctatag tgatggttcc ggctgcgtaa ataccatagc aaacgatttg gtcggctaag
    13561 ctcatgaagt ttggtgtacc caaagcggtg agcgtgcccg gcccctgaat tatcaagtcg
    13621 ccgtcacaca gaatgccgta actgtttatt cctggtccct tacccgattg cagatagttg
    13681 gagccgacca atatgatagt cgtgccgtca ggtaccttta aggcaaaatt gtcaaccccg
    13741 ctctcagcag aatccggcga tgtgattact acgtcggtca atgtaagggt ttgggcttcg
    13801 taatcccagt acacaccgcc ggctgtatag tcttgattgg catataccgc tgtcgttacg
    13861 tcaagcgctt ccgaaatatt tgacgtccac tgtgcggtat aggttgtgtc ctccgccgga
    13921 gctgttccca taaaggcggg ggaccaaccg ttaaaggcat agccctctat tgttcccggc
    13981 gggaattcat tgaaaaagtc gccgtattgg taatcaagct cattgtctcc cgcactgccg
    14041 ccatcagcca aaaactccag cgtaacgtct tttcgcatat agtacaattt gaggacaagg
    14101 ctgccgtcag ctgctatcgt tccggaagca acggtcggag gatcggcaag atattcaaac
    14161 ccgttatagc ttttcgtgct tgccgtcgcc gtatctcctt cagccccgct tagattttcg
    14221 atatccgtaa gaatgtagcc atcatcgaga ttctcaagat aatgctccac cctatacgga
    14281 atcagcccaa cagcttgcca ctgtgccgta taggttgcat tctccgccgg catagtggcc
    14341 gggacagcgg gagaccaacc ggtcaggctg tagccttccc tcgcgactgc gggcgcgctg
    14401 ataggttcgc cgaacttcgc ctggcgggta atgcctcccg taccgccgtt agcgtcaaag
    14461 gtcagcgtat agctgttccg tgtatagtac agcttgagta cgagattgcc gtcagatgtg
    14521 actatacctg acggaaccgt acccggggcg ttacggttat agctaaatcc gccatagctt
    14581 tttgctgttg ccgttaccat ctgatttgcg acgtcggtga ggttttccgt ttcggctaag
    14641 gcatagctgc cgtccaagcc ttctttgtaa tgttcgacct tataggctac ctgccccacc
    14701 gtcgtccatt gggcttcgaa agtcatattc tgcgcgggca ttgtctcggg gacagccgga
    14761 gaccagccgg caaaagagtg ccttgccctt gtaaagacag gtatgttgat ttttccgccg
    14821 aacttgactg tgttttcgac gtccgcgcca atcttaccgt tattcggttg gaatgttgcg
    14881 ctatagctgt tgcggttata atgaaacaca tgcacagtgc tgccgtcagg caaaatctgg
    14941 acgatttccg gtgatggcac taaagaaaat ccttcatagc tttttggtga aacgtgaact
    15001 actgagtcgg tcgtgcctgt acgatattct gtttctgcca gcgtgtacag gtcgttttcg
    15061 aggttctgct tataatgctc caccctgtac ttagtgttag gagccggtac ccacctggca
    15121 tagagtacag tgtcctcagc cggcatcgtg gcagggaagg tatagctttg cgtcaaggct
    15181 tcgtccttat accagccgcc gaatacatag ccagcctttg caggattcgc cggaggtgta
    15241 attactcctt tataaggtaa tgcgatggtg tccaccgcgc tgcctccgcg tgaattgaag
    15301 cttatagtat acgcgctttt caggtcttcc caatgcagcg ggaccgcgcg cgcaatgggc
    15361 acggaagtga aggcaacagg attgcccttc catatgagaa ccaacgcacc ttcggtgatg
    15421 tccttttcgc ctggcgcaac tgtgacggtt ttcgtcgccg gatcgtacgt aaaatcgctg
    15481 tttgtagtac tataatcaaa attagatggg tcataatttt tgctttcaat atctcccgtt
    15541 ttcatatcca tataattcat gctgaagacg ctgtccggca ggacaaagct ttttgtctgc
    15601 ttggccatga aagcggttgg tgccttatcg gcactatact caaagtcata gacatcctcc
    15661 tgcgacccga aggttagcag cggccactgg ttttcataaa gcgtcgcctc gtattcaaac
    15721 atattgtcaa tcgcctgcgc aacaaagctt atttcaagat aaatgccgaa ttccaggtac
    15781 atcgcgccgc catatttgga cgtcaagcct gttgcttgcc gataacttgc ctcgtagtaa
    15841 aaatatcccc ataattgcac ataagcgccc acctcgcctt caatacctat actcgccaag
    15901 tctaaagaaa acaaacctac tttaatctca atgctgatac ccgcgcgcaa gcccagcgtt
    15961 cccataatat aaaacgtgaa gttatactgc tccggcctaa gatctattgt atcgctggtg
    16021 gcagtctggg catttatata tagcgagaat atatatctct tttcgctttc ataggagaaa
    16081 ttgcagccaa tggacacgtt catttttgcg cctaccacaa agtctacatc catcccgaag
    16141 actacgatgt ggaaggggtc aatcgcctgt tcaaaggtaa atatctcctt tttgtatatg
    16201 tcgacccagt cgctgtcatc ttcaagcatg gctttgtatt tttcccggag ggtatcagct
    16261 atagcactgt cctccggctt tttgccgttc atgatgtctt ttatctgctt accaatatcc
    16321 tggaacttac ttaagtcata ttgcgctcct ttttcaaaag tgcctatgct ggcgttgata
    16381 ttgatactgg tataagttcc agtatcgata ctcgcggtca caagagtgtc ttcagcccac
    16441 caaaaccatc catctaataa tgtccatttc gtttcgcagc tgacattcag atcgaccttt
    16501 acctcttgct caaaggcgcc ggacagacgt atgacaatat tattatcctc tcctgccgcc
    16561 tgaaacacga tatcgcagct gatggtcagc gtcacacggg cgccgcttcc gcctagatgc
    16621 tggagataat tgaagaccct agcgttaacc ttgagatttt tcacttcagg cccggtgccg
    16681 aaagcttcaa gcatggacat agcaccatag gaggacgtgt cagccatagc cgtaagattc
    16741 aactgcctaa agctatctgt cttcatcgcc atctccgcca gatactgggc ggcttcgtcc
    16801 gcaaacccgc tctctatcgc ctgccgctca acgctctctt ctaacgcggc tatatccacg
    16861 ttttcaagca gttcatcgcc atcaacttcc tttgtggtat acagatccgt tgcctcccac
    16921 atctgctccg gtgtcgtgga tatgtaggct ataatatagt tatctccgga aacagtcaca
    16981 gacgtgattt ccgcataacc ggcattttcc gcatccgccg atccaaaggt gccggtatgg
    17041 aaggcgataa agtctccgac gtcaacagtg gtagcggcgt taagtccaat atccgcatat
    17101 atggcatcgg tataagccat aacactttga gggacggtga cggagttgtt atcggaagag
    17161 ccgtccgtgt ctgctgtggt actgaccggc aggacatccg gcgtaaataa aatatccttt
    17221 tcatccgctt ttacatagga ataggttgtg ccgctgatgg cagtgatagt aacataggca
    17281 atgtctcccg catcgtcacc gctcgtatca agatcgcgtt ggtccggcct gattccctca
    17341 taaatggcaa ccgtatcgcc cacattgatg ccgccgccgt catatacaaa ggtacccccc
    17401 tcggcgtcaa taccgctcag gccttcgccg tcgccggcta cccgtaccaa cggaatggat
    17461 agtgatttga cggaaaagcc attttgcgtc aaatcggata tctgtccggc agataggtat
    17521 ttcatattct tgttgaggct gagattcatc acgggcgact tggcaatggt gaaagtgcaa
    17581 atcctcgtgg attcgtcctc tcccttgaag gaaaggttgt cgttatcgag agtcagctta
    17641 aaggtgccgc cctcgtcaaa agcgcctcca attgcagata cgatgaattc tccgtcagag
    17701 gattcatcca cttgaatgcc cgcaaaaccg ggatttgagg gagaatcaaa ggtcatgccc
    17761 gctttgactt ccgctgcggt cattccgtct gttgaatcca caactattat tgtaaaggtc
    17821 ttatcctggt caagcttgct tatcgtcggg acgctcagct cttcggtgaa accggttgaa
    17881 gccgcatatt tggcatataa cgtcatgttt ccggtaataa tgtcggtgct gctcaccggc
    17941 gttgaaaggc tgctgtcctt ataccaaccc aggaaaacag cgtccatttt atatggaacc
    18001 gggagattgt caagtgcggt cccgggtttt agtctcagct tatcaattgc atctccccca
    18061 tttgttacga aagtaatggt atatttagtg tcagatctgc tttcgtccct atcgtttcct
    18121 gagccctgtg atgacgccgg tgtcggcgcc ggtgccggcg tcggttgttt tttctcctct
    18181 tgctgcttca ccgtcttgct tatgcgcatg cagaaagctg cagcttcagc gcgggtagcg
    18241 ttggatgcag ggttaaaatt gccattctca tctcctgcaa gcagccccgc atgccaaagg
    18301 cttaatacag aatcaagagc ccatgaagaa atggcgggtg catctttagg tccggatgct
    18361 atctgccttt cagcttcgta agatatatta cagcttttaa aatatcttac cgtcattgtt
    18421 gccatctgtt ctcttgtaat agcttcatcc ggcgagaaag tatcattgtc tgtgccgtgg
    18481 gtgataccgt tttctgctgc ccatgccaca taaggtgcat accaagcgcc aaccgccaca
    18541 tctttaaacg ccgatgtgtt gtattggctt gtatcaacac ccgcaaccct tcccagcacg
    18601 gtcacataca tcgcgcgcgt caaggaagta cccggcgaaa atgtaccttc accggtaccg
    18661 ccaaaaatgc cattttccaa acaatacatc acagattcat aaaaccagtc ggctttgtta
    18721 acatctatgt actcattttc gggttcttgt tttaaactgt ccgcaagcac agccgttggc
    18781 agtgcgagca gcaacataga catcacaagc aaaaaactta ttattctctt taccatgtta
    18841 aatggtcccc ctttcttata tcaattctca attcatttct gaatgtttta atcccgtatg
    18901 gcgaacagaa tgttatccac cgtcagacgc aagggcataa tacttttctc gcccggtacc
    18961 ggctcgggcc agcttatggc tttttcctcc actgtgacgt tcttgaaacg gtttaaatct
    19021 tccagactgc tgaaaggtat tgtttttatg atatcgcgca tattgaagat gatcttgttg
    19081 ccgtgctcga attccaccag cagggtataa tcttcattgg gcgtgacctt gagtatttta
    19141 ctcataatac gaagtctcct tcttctattt ggcggatgtt tcggacataa aaaatccccc
    19201 atactgagaa gtatagggga taatttttta tttctatatc taccgaacgg tatatttttt
    19261 cgaaaaatta caaaagcttc aacttttgcg ccttttttat ggctgccatc ctgccgctga
    19321 cctccagttt caggtagata ttatgcagat gagtctgtac ggtgcctgcg gtaacatgaa
    19381 gctttccggc aatctcgtcc cttttgaggc cctccgctgc cagggtaagg atttcaagct
    19441 ctcttgccga cagggaaact gcgctctgta tagagtgctt caggctctcc atataatgca
    19501 cgcaggcttc aagaaccttc tttatgtatg cgtcccgcga atcagcatgg gcgatgtgcc
    19561 tgaccatgtc tatgacggcg ggcgcatact cggcgaaggg caggatgatg tggtcttccc
    19621 gggccatgct gaatgcttcg cggagggctg cgcagccaac ctccatgccg taaagaggat
    19681 atttcgcagt tgccctgaaa atctgattgt gcagaaagcc gagctggttg tggaagacgg
    19741 cgaaacagcg cgcaaattct tcagtgagca tttcgagttt gatgtaattc tttgaaagca
    19801 gaagcgcttt gccgtagacg atatatttaa agcccagacc ctcgtaaaaa aattgcgcgg
    19861 gggacatatc cccggtttgc agccacaagg ggatgctgtt tagccgggtg aggcagccgt
    19921 aaacgtaccc ttccacgatc tcaagcccgg tgttgtaata gaggttgttc gtccgcttca
    19981 catcctgcct cagctgccgc agaagctcca atgcttcatt ggtcttcccc ttatagagat
    20041 acagcctgat caaaacaaaa taagcgcaga tcacaagacc gatctgttcc tttgtctgcg
    20101 ctttataaat gcctttaaag gcatccaact ccgccgcctg ccagttgccg gtttccagcg
    20161 cgtattcggc tcgaaccaca tagtcgcagc cggtgctgca gccatcggca agctcggcaa
    20221 aaatcggaaa atccgatgcc ataagctctg taagctcttt gaggctgccc ggctccctgt
    20281 aacaggtata gagaagatgg ggagagccca aggtgaactc tgcttcccgc ttttgcaaga
    20341 aggaaacatc accgtcaagc aggcgctgtg ccttctctat acaagcggcc atttccttag
    20401 catcattgaa cacggcaaaa atctgagtga gataaagttc cgcaagaacg cggtctctgc
    20461 cgtgcgggac ggggtcttcc gcccgctcgc aagcctcccg caactcgttt aagcgtatca
    20521 caccgtcctg aaaatcgtcg ggatcgccgt ttgttaaaat gatggcgata tactgcaaat
    20581 aagcaagcgg atatttatga agcatctcgc ggggcattgc ggcaaacaag tctaaagcgc
    20641 cgtcaaaagt ggcggaatcg ctggtgatgg tatccccgtt gtccagcagc gcgaggattc
    20701 gctccgactc acccgcgcgg tagagataac cgtatgcagt tctatatttc ctttgtgcaa
    20761 ggtaccattc accaagccga cgatagagcg cggcacgttc ttcttcatcc ttctgcttac
    20821 cccgaaggaa atccagcagc acattgtgta ttatatagac ccctgccgcc tcgtcgaagg
    20881 caataaatgc gttttcgcgg cgcaactttt taagggtttc ctccgatttt tcttcttgcg
    20941 ttacaaacac agcctgctcc gccgtgaagc tgtccatcac ggaaaggcac agcaaaaacc
    21001 tttggatgcg ctcatcgtat gcgttataga gcaccttctc caccaactca ttgattgtgc
    21061 cgtttcggcc cacgggaatg ccccgttcca taccgagcat gataagataa atcaatgaaa
    21121 tccagccgcc cgtgtaatct ccgatttttc ttatctcgcc ttcgctggcc gtgaaaccca
    21181 tcagggcgca atagtcccga atctcactgt ctgtaaaccg cagcatattt tgagacacaa
    21241 tatagcacag cccttttgcg gaaagctcgg tgatgtcaag atttgtcgta tcacgggtca
    21301 agatgacaat gtgaaaatcg tccggcattt ccattacaaa gcgtctgaaa agtgcagtca
    21361 cccgcatact tcttgcaagg tggaaatcgt cgatgacgag cgtggtatcg ggcttgtaac
    21421 ccatctcatt gatgatggaa acagccattg ttgtctgcgg tgtgtctgca ggaacgccga
    21481 gacttttaag cctgctctcc gccgccttat caaatttacc gatttcggcc gcaagccttt
    21541 cccaaaaacc atgcgccgtg tcttcttcgg ataaaaagga agtccatatg acaggcacgc
    21601 cttttaaagc aaggaattca ctcactgcag tggtcttgcc gtagcccatg ggcgcttcca
    21661 caatcgtcag cggatattta aatatattcg tgagttcacg gttaatgcgc tcacgcttaa
    21721 gtgttcttcg ctttttcatg aacttacttc cttccgagct aagcaatcaa catggattga
    21781 ggatttgctt catttttctg tcaataagca gcgtccaaag tttattgcag cttaccgcca
    21841 tggtaattta cctcgaaata aatctcttcc ggatttacct atgttgatta tatcacgtcc
    21901 cttgctcgaa tacatctatt taacacaaaa aaagtcagaa ttatcatatt tatgccaaat
    21961 ttagcaacta tctccgaaaa gtgccaaaga agccgagaaa gtactggggc aacagtaaag
    22021 gcagccagct taatctgctg cctgcctcgg caaattaccc acggttcaca ttatgtccct
    22081 atggaatggg acatttccgt ccccctgtta ttgtataccc tctatctttt gtttccaaag
    22141 aagaaaagtg ccactacacc cggcccggta tgtgctccta taaccggccc tacatgattt
    22201 attattacat cctttacatt atattccttc agtatcattt ccttaagata ttctgcttcc
    22261 tcgatgcaat ccccgtggct gatggcaatt acctgctcct caggagcctc tatattttcc
    22321 tgaagcattt caaaaagcgt cttaatggat ttctttcttc ccttattctt tgatatcggt
    22381 atcagctttc cctcgtcatc aaccttaaga acaggcttga tatcgaggag cgtgcccaga
    22441 aaagcagctg ttccggataa ccgtcctcct cttctcagat gccccaaatc atctaccgta
    22501 aaccaatggt tcaccttgag cttgtttgtc tcaagccagc ttaccacttc atcttttgaa
    22561 gctcctgcct taagcatctc atttgcattg taggccagga gcccttcccc gagggaggca
    22621 cttttactgt caactattgt aatatcagca tccttgattt cttccataac catatccctt
    22681 gcaagatatg cgctgccgag acttccgctc aaagcagaag aaaaacccag atatataact
    22741 gatttacctt ctgttgcata cttcttgaaa atctcagtat attcataaac gttaacctgt
    22801 gccgttgaag gcatttcacc gcttctcact gcatcaaaaa attctttata gctgatagac
    22861 ttgccaaaat cgtccttata ttccttatct ttaaagttgc atacgagact gacaaaaggt
    22921 atcgcatttt catccacata gctgcgcggt aggtctgagc aggaatcaat caaaagcacc
    22981 gtatccatca tgttctcacc tctctgcaat attatattac tactatagta accatttttc
    23041 aggtataaat caataagaca atcagccttc agggcaaatg gatcaaatta ttgccgtcac
    23101 tgctgcactt tcacggcact tccttctttc tcccgcattg tgttctccct gtcctcatac
    23161 ttttcaagtc caaacatcaa aacagcagtc atggccgtaa atatcccaat aaatatccat
    23221 gctttttcta tgctaataaa attcagaatc ctgcccatag ccatggggcc aaccgcatag
    23281 cccgtacccg ttatggtagt caataccgca ctcatccttc ccctgtgcga tgccggtgta
    23341 tgattcacga taaaaggcat gacacttatt gtcaatagca cctctcccaa agtgaaaatc
    23401 agagcggaga caaagaaata ctgtagcttg tatattagcc ccagcattcc gaagcctatg
    23461 gcataaagca gtatgccatg gaccatgatt ctgatgcccc ttgtttttct tgtgagcctg
    23521 gtcagaatcg gagtaaataa cataaccacc aagccgttga agctcgctat ccatccaaaa
    23581 tactgagcac ccatttcacc gaagttcttt acggaatgca gcggcatcaa aaaattccac
    23641 tgggaatatg aaaattcata tcctaatgca ataattgaaa aataaataag aataggcctt
    23701 ctgagcagca ctgaaataat tgagcccttt tccctttttt cgagcttcct gctttcatcc
    23761 gttatatcat tctttgtgct ttctatggtc tcattgataa aaaagtaaat aaggcctgtt
    23821 gctataagcg cagttactgc atcccctatg aatacaaggg gcaggtatct cctgtaaagc
    23881 aaacctccga atattgggcc gacagcaaag cccatgttcc agccaagata gcttaatgaa
    23941 taagcctttg ccctgttttc cggcgtggta aggtcggcta tcagggaatc atgtgcgggt
    24001 ccggctacaa acataagcgt acttgccccc aggatgacat atataagctg catggagggt
    24061 ttaataaaac cacaggacat atacaacaaa gccccaaggg ttccaaatat tattataaga
    24121 tgcttcctcc caaaggtatc ggcaagcttt cccccaagga ttgaagccac gggaaaaacc
    24181 atgctgttca ggctcaggaa aaagcccgca gcctcgtttg acaagcctat cctgtcggtc
    24241 attattattg tcataagcgg cattacaaag cagcccatgg cattcactat acgggatatg
    24301 aatattatgt atatttccct cggcataccc ttatagggac tgattaaatc ctttatcaac
    24361 gacatattgt acttcccctc tcataaaaaa ataaaagcca tggaacaaac acattccatg
    24421 gccaaaaatt tcacggtgtc tcctccttag agcagcccgc agttatatat ccatcaaccc
    24481 taaaaaactc tcaaagcagg ctgcgacact atattatttt ttatgtctgc aattatacta
    24541 attctcatat tcgcaacctc cttttccatt ttttccttta cccttgtaag tatactccag
    24601 tcctttcatt actgtcaatc agcaagcaaa attgtaatac aatattaata atatgctaat
    24661 gtttcgcatt ggcaggctca atattgattc tcctgccttt tatctggtta tccttcatta
    24721 ttcttattac atcgccggca tattctctcg gcacttcaac aaaggtgtat ttgtcatata
    24781 tgtcaatgct tcctattagc tttccgggaa tgcctgtttc ccctgcaata cctccgacaa
    24841 tatcccccgg ccttatcttc tgatttctgc cgatgttgat gaacagcctt accattccgt
    24901 cctccgctcc ggtgtcatta aaatcagttt cctcatgctg cacttcctta tccttattcc
    24961 cgacagccat ctttagaagt gcggcggcga tttcaatact ggtatagtct tctcctgcca
    25021 gcctttctat aatactgaca tactggctga gatcatcttc atccatgatt tctattactt
    25081 tatccaaaaa taaatttgtc cttacctctt ccacatcttc tgaggaaggt gccttctgca
    25141 gggtaatctt tgcctttgca tacctctgaa tatctcttaa tttaaatatc tctctgccca
    25201 caacaaaggt aaaagccctt cctcccctgc cggctctggc tgttcgtcct attctgtgta
    25261 cataatcctc ttcatcctgg gggagatcat agttgaatac tgcttctata tcatccacat
    25321 ctatacctct ggcagcaacg tcagtagcta ccagtacgtc tacagctccg ctcctgaatt
    25381 ttgacatcac cctgtctctc tgaacctgct tcatatctcc atggaggccg tccgcaaaat
    25441 accccctgcc ctgcagctct gccaccagct cgtctacctt tcttttagta ttgcagaaaa
    25501 ccaacgaaag ctttggatta tacatgtcta taagtcttga cagcacttcg agttttgtct
    25561 tttccttcac ctcaaaatat acctgctcaa ccttgggtac tgtcagttcc ttatgcacca
    25621 ccattacatg cttaggatcc ttctgaaacc ttttagtaag ctccagaatc ggcttgggca
    25681 tagttgccga gaaaagcatc gtctgccgtt cctccgggac aacctttaat atggtaacta
    25741 tatcatctat aaatcccata tccagcattt catccgcctc atccagcaca accatcctga
    25801 ccttgtctat tttcagtgtc cctctgttca tgtgatccat aacccttccg ggcgtcccta
    25861 caatgatttg cggttttctt ttcaatgcct ttatctgcct gtcaataggc tgaccgccgt
    25921 atatgggaag tacctctact ccccttttga acttggccaa ttccctgatt tcttcagcga
    25981 cctgtatggc aagctctctg gttgggcata acactatccc ttgaactgtc ttttctccct
    26041 ggttaaactt ttccagaatc ggtataccga aagcagctgt cttgccggtt cctgtctgag
    26101 cctggcctat tatgtccctt ccttccatta tgtaaggaat agattgggct tgtatcggag
    26161 ttgcctcttc ataaccttta tttcctattg cctttaaaat ctcatcggat aaatttaatt
    26221 cttcaaactt cagatttttc atttactacc ttccaatctt ttttaatgta actatataac
    26281 aatacattta ttatgctcaa attgcaactt tttttttcat attagaagaa taatgcgcca
    26341 taggtaacgg gtaacacggg agacacggct gtttcacgaa atcgctgcgc gattgcacgg
    26401 taactattat atattatata gttgcataag aaaatttcga tacttatatt attatcgata
    26461 attatttatg taacgtaata tttatcccgc cgtgcattta gcggtttttc gtgtcttcag
    26521 tgttatcata actccgaagc cctgccctaa acctcgaacc tcgcgcctcg tacctcgtac
    26581 ccgaagaatg cgtcgcattc ttcttatatt atgcaaatgc aaaatccatg attgatatct
    26641 caaatttttg tcaatctatg atataatgtg tatggtcata tactgccgtc ttgagaattt
    26701 atacaaaaga aaggaagtaa tgtgatgagt ctggaaagtg taagaaaatt ctttgatgaa
    26761 aatggttatt cggacctgca gattattgaa aaagatgcaa gcactgcaac tgtcgagctg
    26821 gcggcaaaag cacacggtgt tgcacctgcg cttattgcaa aaacaatggc ctttggattg
    26881 aaagacagaa atattcttat agttgtaagc ggtgactcaa gaatagataa tcgcaagttt
    26941 aaagactgct ttcatgaaaa agcctcaatg cttaacccgg aaaaagtgct ggaagcaaca
    27001 ggtcatgcag tgggaggagt atgccccttc ggcctcaaga atcccatgga tatttacgtg
    27061 gatatttcaa tgaaggaatt tgaagctgta tatcccgcag caggatctgc aaattcagct
    27121 gtaaaaataa cccctgaccg cttacaggaa gtcacagggg gtacatggat tgatgtatgt
    27181 aaatagagaa tctatcccaa ctacactacc tttgtatctt taatcatagt tttcactatt
    27241 tctacggcat cttctatata agaaggaatc ggttttattt ctataccggc tacagcaata
    27301 ttacatttac tcataggaat aagtatcttt tttgccggac tctccgctat agcctttgcc
    27361 ataagcggtg tcaactcacc taacagtgaa tttgcgctta ttattcctat ggggcccaaa
    27421 attatatcca ctttgcaggc gttatgaata atggcatttt ctcctgttgc accttcatta
    27481 gcgcctgctt tcagcatcaa tgaagttgcc agagagttag ttcccaaagc cactatttct
    27541 gctttatctt caagtttctg cctgattctt tcaactatag cttttccaat tcctccaccc
    27601 tgaccatcga ttactgcaat tcgcatatta tccctccaat aagtcataaa taaatccttt
    27661 acatcaaggg tgcaaatatt ctgaggatcg ttctgcccaa ccttttatac aacacttcgt
    27721 ctctacagca tcctaaagta atcctttggc aaacctttag agtttttaga aaatccgctt
    27781 taacttctaa gatactattt gttttataca tccagacacc gcactcaaag tgaagataca
    27841 aactgcgata atctaagttt attgtaccaa ctacagcata cagatcatcc actacaaagg
    27901 tttttgcatg gataaaaccg ggtgtgtact catatataga aactccgctt tctatcaatg
    27961 cttcataatt gcattgggat actgcatgaa cataccattt atccggaatg taaggggttg
    28021 tgatgcggac atctacacct ctttttgctg ccatacacag agctgaattc atctcattgt
    28081 caagtatcag gtatggcgta ttgatataga tatatctgtc agcttcacct atcaaattaa
    28141 ggtatattgt ttcacctacg gcttcctcat caagaggact atccgcaaaa ggttgtacat
    28201 aaccattata gctaggatct ttagtatatt cgtaagtcgg tctaaacttc tcgtagtcct
    28261 catcttcttt tcggagaaaa tcccacatgg aaaggaacat tacagtaagg ctccatactg
    28321 catccccttc aatggcaata gacgcatcct tccaatggcc atacttttca taagcattga
    28381 tatattcgtc tccaaggttg ataccccctg taaacgctac acgaccatca attacggcaa
    28441 ttttacgatg atcacggtta ttgaggcgga tagacaaaac cggcacaaag ggattaaata
    28501 cacaacattt aatgcccatc cgttccagtt ctttatcata tccataaggc aatgtcaata
    28561 tacagcccac atcatcataa atgacacgaa catccaagcc ctgcattgcc ttttctttca
    28621 aaatatccaa tatggtatcc cacataacgc ccttatcaat aataaaatac tcaagaaaaa
    28681 tataacgctc ggcttttttt aattcctcca acatgcgttc aaattttctc tcaccgggtg
    28741 taagatattc tgttcttgta tttgtatgaa caggacagga agaataattc tctatatatt
    28801 ttgactgatt ggcggcgcct ctgtcttcct tctcaagatt ttgcatgact ttcaaattct
    28861 gaacgaggac attttgcatc ttatcagtaa ttacctgcat ttttcgttta ttccttttgc
    28921 ttgacttatt tccgccaaac atcaaataaa aaagtccgcc gaaaatcgga aaagccatga
    28981 taggaataat ccaagcaagc ttatatgcat gattactctt cccattaaca atacacagca
    29041 caacaactat actaagtaat gtactgaagg tatagaaata tacaaaataa ttgctgaacc
    29101 tccatatcat tattaacaag gcaaaaacct gaataaatat ggcagtccca accaaaaaaa
    29161 ccctgctaaa tatcattctc aacagctttt gcataataac ctccatcgga ctccgtttta
    29221 tccataccct tatgtttatt atacagttct aatcggaaca tagcaaaata tatattgata
    29281 ttactataat tgacttaatt tgctgcataa cttatattta aacatagaaa caactatatt
    29341 atctaaggag actgatcatg aacatccttg taacattaaa ttcaaattat ataactcctc
    29401 tgaaggtgat gctaaaatcc cttttcttca ataattggag agaacgcttt agcatttata
    29461 ttatgcaatc cagcttaaca gatagagaac tgcagcaaat agaagatatt gtaattaacc
    29521 aaggacatga atttcatgtg atatcgataa aagatgagta ttttaaggat gcaccggtag
    29581 tcatgcatta cagcaaagaa atgtattata ggcttttggc atttaagttt atgcctccga
    29641 aaatagacaa gcttctctat ctggatcctg atatattgat tattaatggc attagagagc
    29701 tttataatac tgatctgacc ggttaccttt atgcggctgc atatcatcat agggggtcta
    29761 tcaacaaaat taacaaaata agactaaaac cttatgatat tgaagcttac tacaactcgg
    29821 gagtgcttct ttttaacctg aatcagcagc gtaacacaat agatgaaaag gatatctttg
    29881 attttgttag agaaaatgcc gatacactaa tactcccgga tcaggatata cttaatgccc
    29941 tttactccaa aagcataaaa ccggttgatg aaattctata taattacgac cccagatatt
    30001 acaattatta taaaattata tccaatggca agtatgatat gaattatatt atttacaaca
    30061 catctataat tcacttctgc ggaaagcaaa aaccatggaa gaagaattat tccggcaaat
    30121 tccactcact gtataagcat tatgagagaa tagcattaga gcagtcttga tatcaataac
    30181 agccggcttt atttttatta atcctataag acttattgtc tattgctctc tgaactgcca
    30241 atataccatc aagatgatcg tattcatgct gaatcaattc tgatatatcc ccttcaggtt
    30301 caaaaatgca atccttccac tccagatcct tataatagac tctgcacttt ttatatcttg
    30361 ataatcttac ttccaatccg ggaaaactca tacagtcatc ccacatttca aatttttcat
    30421 cgtcagtaaa ctcaagcctt ggatttatta atgctatttc tctgtcatca aaattcatgt
    30481 agattatccg aaaaaactca ttaatctgag gcgcagctat agctcttcca aaaccgtatt
    30541 tttttctgaa atctgccatt gtgtcatgca aatcgtcaac aatttgtttt gctttatcta
    30601 tttcttcctt tgaaacctct ttggatacat tgtatagatt ttcattcccc agcagcaata
    30661 tctctctgac ggccataatc ttccctcctt gatgctgcgg caggacgcaa agtttttaaa
    30721 ggtcatcttt tcgcctattt atatatatta tacagtttta atcaagtcct ggcaaaagaa
    30781 cctttattat cttagtatta tttttttctg ttccggacaa attaatatcg taggaacata
    30841 acggttgagc caagactcat atatgctcct tagcgggggc ttgtatgggt cggccaaaga
    30901 ttgtatatgg gtcgaaaggc ctgtgtgcag tcggagggaa gatcctgtat ggtaaggaaa
    30961 ggttctgtta tagtcgacta cccttgatta tagtaggatc ccaacttatc atacagggtc
    31021 gagctaagat tatgcagggt gccgtaatgg tcatatacag ccgggaggtt gtatgtggct
    31081 gtgcaggtac tttgcatggt cgaacgaacc atcattgcag gtggtgaact gcttggtcat
    31141 ggtcgaaaga tcatggctgg gtaataagct gtctgcattg gtgcaaggta gatcgttatg
    31201 ttccttttat tttattttct cataatcgcc aagctttaca tcgtcaatga gccaacggtc
    31261 ttcaattttc ctgactgcaa gagtgatcgg ccgctttgcc gatattccgc cctctgcctt
    31321 ttcagtgctg gttatctcga ttatttcacc ttttacctca tactcatttt ctgccgattt
    31381 ctcaatagcc aggatctcta ttctatcggg ccaggggctc gaagtcagcc ttcccggagc
    31441 attcatggga tcctttgccc attttgcaat aagctctgaa gtcacaaaat cgccataact
    31501 ctcttccatg ctcttttcca atacttcctt tggagccaga agcgagacct cctgaagctt
    31561 tttcccaaag ccctcaacaa tatttgctgc tgcttcctta tcggcttcat cagtttgctg
    31621 cgtcttttcc tcattctccc gggactgact tcctgctgcc gtacagccga tcatcaaagc
    31681 gctcagcaac agtattaaag caaacaacaa tagttttttc atatgatacc tcccaaatag
    31741 tctttgatta atagacgtaa taagcaccct tttcgttcca tcaatcaagc acttctttaa
    31801 gccttaatat gccattcctc aatccgcccc ttgtgaatag taatgcccaa tgctataata
    31861 tgcagcataa tacactatat gacaaaataa tcaatgaaag aggtgttcct atatggtact
    31921 caggaatgca ttagacctga tcagcaacac gccggttgta gcccttgaca acataacagg
    31981 gaatattgag ggaagaattt ttgcaaagct ggagttctgg gctcccggtt taagcaagaa
    32041 agacagggtt gcattgcagg tgattgagga tgcggagaaa agcggaaggc tgaaaccggg
    32101 ccagccagtt gttgaactga ccagcggaaa tatgggcacc ggattggcta tcgtctgcaa
    32161 gctgaaaggc tataaattta tagccgtgat ttcaaagggc aattctatag aaagggctaa
    32221 aatgatgaaa gccttcggtg ctgaagttgt ccttgtggat cagatgccgg actcaattcc
    32281 tgggcaggta tccggaaagg acctggaact ggtggaaatc gaagcacaaa ggcttacaaa
    32341 agagctgaat gctttcaggg tagaccagtt ttataatccc agcaccgctc gtgcggggga
    32401 agtcggggtt gccaatgaga taattcagca aatagatgaa agaatcgatg ctttcgttga
    32461 tttcatgggt accggaggca gctttatcgg tgttaccaca acattaaaaa aacacttccc
    32521 cgatataaag tgttttgggg ttgaacccga aaatgcaagg cgctacagca atcctcagga
    32581 tatcggaagc ggaaagcata agatacaagg gggaggctat aatatggagc tgcccctgat
    32641 gaccggggat aagctgaagc tggtagacgg tttcataacc gtgtcagatg aagaagcaat
    32701 tcaggcagcc agaaggctgg catgtgagga atgcattttt gcaggcttct cagccggggc
    32761 gaatctggca gcggcactaa agctgcttaa ggtagatttt aagggaaaaa acattgtaat
    32821 attgctgccg gactccggca caaaatatct gagcacagat ttgtggtaag aatacgtcgc
    32881 attcttcagt gtttatttca atttggagca aatcagcctg cctaaccttt caatgcctgt
    32941 tctgattctt tccggagcag cactgccgaa actgatcctc atacagtcgc tgcccgcgcc
    33001 attaccttcg gtaaagaagc cttcccctgc cacgtaggca accttgacat cgggatttgt
    33061 aacagcttca gccaacagct cagtggtatt gatgccttcc ggcagctccg cccatgtgaa
    33121 caggcctccg tccgggaagg ttctcttggt cccttcggga aaatacttat cgatacattc
    33181 aatcatgatg tctcttcttt cgcggtacaa atcacatatc atcttatgat gggccggata
    33241 atacccgcgt ttgaagaatt cggcgcatat tacctgaggc agcatggatg tgtgggaatt
    33301 ggttgccgtt ttggcatcaa acaatcttga tattatttca tcatttgcaa gagcatagcc
    33361 cagcctgctg cccggcgaga atatcttgga gaagctgttt gccaagacag tgtgaccggt
    33421 ctggtcaaag gcttttatcg gcagcaagtc gcttccgctg taacgaatgt cgcgataggg
    33481 atcatcttcc agaataatca catcataacg gcttcccatt tcagcgattt tctgccttct
    33541 gtccaaggac agggttctgc cggttgggtt ctggaatgtg ggaataacat acaccatttt
    33601 gggtctatac tttaatattt ttgcctccag gtcttcagga atcatgccgt tatcatccat
    33661 atccacagca atgcaatttg cctggaacat ttcgaatatt tcaacacaat gtacaaagga
    33721 aggtgattca accagaatca catcaccggg atcaataaac agctggcaca ttaggttcat
    33781 ggtttcaagg ccgccgctgg taatcataat attatcaaca cttgcagcaa ctccctttgg
    33841 tgccagcaaa tcattaacaa cagcctcccg aagatccgcc gcacccataa cgggaccgta
    33901 ctgaagagcc tccgcgcctc ttttgtcagc tctaatcact tcatttgcta tttcacggac
    33961 aatatctatg ggaagtgcct cttttgccgg agcaccgccc ccaaaggaaa tgataccggg
    34021 gtcattcata gcaccaaaaa gatttcggat aatctttgcg gatttctcca ttgcagtcat
    34081 acgctgcgca aaatttgaca caataacctc tccttttaat tttatatatt agtatcaaca
    34141 tatcatttga tttcagctac aaatttcctc gtgcacaaac agcctctgtc ctgcagccgg
    34201 tcagctttat taaatcctcc ggggctaatt caatctgatg tcctatcttt cctgcgctta
    34261 cgattattgt atccatggct ttacaggagc tgtcaatcac tgtcttatac tgctttttca
    34321 tgcctagggg agagcatccc ccgcgaatat atccggtaac tttattgatg tccgcgactt
    34381 ttatcatctc aacggctttc tcccccacag accttgccgc tgctttcaaa tcaagctcag
    34441 cggcaacagg gattataaaa acatagaatt ccctgcttgt cccctgggcg acaagggttt
    34501 tgtacacttt ttcaacaggg cagccgattt taccggcaac tgaaactccg tcaatcaagc
    34561 cgtcgctgtg ttcataagca tatgtattat acttgattcc tgccttttca agaagccgca
    34621 ttgcatttgt ctttaccttt tccaattcta cccctcactc catgaatatc gtgaacaaag
    34681 gaagctgaac taaagtgttc ctagagcctg gttctcataa tgagagttga acgcatccgt
    34741 ctgtaatctg ttgttacata ggagcctact ttatgagcct caaacttatt catgttttca
    34801 aatgctgcgg aaatcataag ttttatcctg ttcagctgat tcacttcact tgcacccggg
    34861 tcataatcaa tagctactat gttggttccg gggtaaaccc gtttcagctc cttcatcatt
    34921 ccttttcccg tgacatgatt cggaaggcat gcaaagggct gcatgcagat tatatttctg
    34981 actccgttat taataagctc aaccatttcc cctgtcaaaa gccatccttc cccggtctgg
    35041 tggcctaaag acacaactga tgaggcaccc tccgctatct cttctattgt ttggggagca
    35101 taaaaccttt tgcttgcaga tagcgcctcc ctgtaagcct tcctgtagaa ctcaataatc
    35161 ataacggctg cacgcccaaa gagctgggat tttttgctcc ctgacaaata tttatatcta
    35221 tactccaaac cataggcact atacagcaga aagtccgtaa gtcccggcac tactgcttcc
    35281 gctccctcgg cttcaactat attgacaaca ttattgtttg ccgtaggatg aaacttgaca
    35341 agtatttccc caaccagccc aacctttggc ttagcttgat tgatcatttc caattcatca
    35401 aattctttaa caatttgtct gataatctgc ttgaatactt ttctgctggc atattccaca
    35461 gattccttac atatttcagc ccacttctca tacagcatat tggcggagcc cttaatcctt
    35521 tcatagggcc tgactctgta cagtacattc attagcaaat cgccgtatac aagtcccatc
    35581 atggctctgt tcaaaagctc cgttgtgaac ttgaagcctg aattcttctc catacctaca
    35641 gcattcaacg atataacggg aactttctca aaacccgcat ccttaagggc ttttctcagg
    35701 aagcctatat aattggtagc cctgcagccc ccgcctgttt gagatatcat taccgaagta
    35761 ttattcaaat catatttgcc ggacttcagt gcttctataa gctgtcctat tacaataata
    35821 gcagggtagc aggcatcatt attcacatac ttcaggcctt catccacagc tcctttatcc
    35881 attgatgcca aaacaaccat attataacct gatagtctga acgccttctc caggaactga
    35941 aagtgtatcg gagacatttg aggcactaaa atagtatgct tcgttctcat atcctctgtg
    36001 aattcaattc tcttatatct gttgtcaact ttcttaagct caaacccgtt cttatgcctc
    36061 tccaaaattg tagccttaag tgaccgaagc cttatcctgg ctgctcccag attattaccc
    36121 tcgtctattt tcagggttgt ataaatcttg gaattctcat taagtatttc ctgcacctgg
    36181 tctgttgtaa cagcatccag cccgcagccg aaggaattca actggaccag ttccacgtcc
    36241 tcctgctttg cagtaaagct tgccgcagca tacagcctgg agtggtatgc ccattgatcc
    36301 actactctga ggggcctttc aacccgtccc agatgggcta cggagtcttc cgtcaataca
    36361 gccatgccaa gggaatttat catctccggt atgccgtggt ttatctgagg gtctatatgg
    36421 tacggccttc cggcaagcac tatccccttt aatccattag ccctgatata actcaaggct
    36481 tcttctccgg ccttttgaat atccctctta aaagcttcct gctccatata agcggtatat
    36541 acgctttcct taatttccat cttactgatt ttgaacatct taaactcttt atgcagtctg
    36601 tcgacaagcc tgtcttcgtc atcgaaaggc aggaagggct tcataaacag tatacccttt
    36661 tccctcagca catctatatt gtttttaata acctccggat atgaaaccac aattgggcaa
    36721 ttaaaatgat tgtccgcttt cttttgctct ttcagctcat gggttatgga tggatagaaa
    36781 atgaacttaa tacccttctc aacaagattc atgatatgcc cgtgaaccag tttccccgga
    36841 tagcacaccg actctgaggg tattgtttca attccttttt cataaagctt gtttgaagac
    36901 ctgtcggata attccactct gaagcccagt tccgtaaaca gggtaaacca gaaagggtaa
    36961 ttttcgtata tgttcagaac ccttggaata ccaacagtac cccttcttgc taatgagctg
    37021 tccaagggct tgtatctgaa tactctttca tatttatact tatacaggtt cggcagtaaa
    37081 ttctgctctt ttatcttgcc ggttcctctt tcacatctgt tccctgatat aaagctctgc
    37141 ccgtttccaa acttgtttat tgtaagtaaa cagttgtttg agcataaccc gcatcttgat
    37201 accaccgttt caagattgaa ttcattgagt tcatgttctc ccagcaagct tgtatgcatg
    37261 cccttctgat atctttccct tgcaatcagg gcagatccaa aagcacccat tattcctgct
    37321 atatcaggcc ttattgcctc tcttcccgat aggagttcga agctccttaa caccgaatca
    37381 ttataaaagg tgcccccctg gacaactatt ttttctccca gttcgttcag gtttctaact
    37441 tttattacct taaacaatgc attcttaatt accgaataag ataatcctgc tgaaatatcc
    37501 cctactgtcg cgccttcctt ttgtgcctgc tttactttag aattcatgaa tacggtgcac
    37561 ctggagccca gatctaccgg attctctgca gacagtgctt ccttcgcaaa ttcttcaaca
    37621 ctcatgccca atgagtttgc aaaagtgtcc aggaatgaac cgcagcctga agagcaagct
    37681 tcgttcagca ttatgctctc aataacccca ttctttattt tcaggcattt catatcctgg
    37741 ccgcctatat ccaatatgaa atcaactccg gggcagaaaa attccgccgc tttataatgt
    37801 gatatcgttt caatttcacc tatgtccaca cttaatgctg ccttcagcag gccttcccca
    37861 tatcccgtaa cagttgaatt aactatcctg gcattcttcg gcaaaagccg atacagctct
    37921 ttcagtgctt taacggtgga ctttaacggg cttccctcat tactgctgta ataggtgtaa
    37981 agtatcgctc cctcatcgtc tataagagct atttttgtgg tggtagagcc tgcgtcaatc
    38041 cctaagaagc attcaccttc aaaggttgat aattcacgcc gtatgacaga gcttttttga
    38101 tgcctggatc tgaactcttc atactccttc tcatcagaaa acaggcgttc caatctttct
    38161 gattccccgc cggaggcact ttcatggctt ttcaggctgc ctatcaatga ttcgaaaaat
    38221 acaggctttt cattaagaga agataatgcc gcgcctattg ctacaaagag ctgcgaattt
    38281 tcaggaaaaa tcacctgatt ctctttaagc ttcaacgtat caataaatct ttccctcaac
    38341 tctgaaagga aatataacgg gccgcccaaa aaagccacat tccccctgat aggccttccg
    38401 catgccagtc cgcttatagt ctgaattaca acggcctgaa gtactgaagc tgcaatatct
    38461 tccttggcga ccccctcatt cagaagaggc tgcacatcgg tctttgcaaa aaccccgcat
    38521 cttgccgcta tgggataaat aaccttgtaa ttctcagcca actcgtttag cccctctgca
    38581 tccactctga gcaaagaggc catttggtct ataaaagaac ctgtgccccc cgcacaagta
    38641 ccattcattc tctgttctat tccgtcggcg aaataagtaa tctttgcatc ctctccgccc
    38701 agttctatta caacatcggt ttcaggataa taggtcttaa ttgcttttgt agaagcaaca
    38761 acctcctgtg taaaagaaac ccccaagctg tttgcaatac ttattcctcc ggaacccgta
    38821 atcattacag tcaattgact gtttttcagc tttctaaagg catcataaag catctgctga
    38881 agctttttct taatatcagc ataatgtctc tcatacctgc tgtaaaccat atcgcagttc
    38941 tcatttaaaa caactacttt aactgttgta gaaccgatat caataccaac atgaaataaa
    39001 ttactcataa ccccacagca tgcaagctgt gcccacctcc acggctctga attataaccg
    39061 ggcaaccgga ataataaatg caaatcatat tataagtatc ttctaagtat catgctgatt
    39121 aaagcttatt aacattttat atgggtacac tgaataattc aataggcaat agaatggcaa
    39181 atgccaaata ctatatgttt ttctatatat attcataata atccacaaat attgataatg
    39241 cgggtattct ccagcctgat ttttgcagat tcccttccta gtatcacttt gtccttgaga
    39301 atcctctcca cagctttttt aagccatccc atctgctcgt cctgtctcca agcctcatgt
    39361 caagcttctc attaattttg tcagttgtgt ctctgattga ttgtgcgtca atattggctc
    39421 cggcatcgag ttccggtaaa tccccttccc catattcccg ggtgtcctga cgttcaattt
    39481 catctattga ttcaaggagt tcctcacact tctccctgag gccttcgcgg aacctcctgg
    39541 gatttttcac ccatgcccag ctgtccttgc ctgcatcagg aaaatacctt tcaagcttta
    39601 tatatccctt actgtgaagc agagctatga cttctgtaaa aatatccgga ataatatctt
    39661 tcatacgttc gattcgaaat ctgtcaatgg ttttataatc gggcatattc attccgctaa
    39721 gccacataaa cattatattt tccctgcaat tcttggcgat gcttctgcat gaaaaaattt
    39781 tctgggtata agcatatatt attattttca gcatcataac aggattgtaa ctgcttgctc
    39841 caaccccttc atatttctca tacaaaggtt ttgtgtcaat gctttctatc gcgctgtcaa
    39901 ctactcttac tatatgattc tcgggtatca aatcctccag acttggagga agaaaggtaa
    39961 tctgcccttg attgtatggt ttgaaggaag gtttcttatc cataatatct ccctttcatt
    40021 tatattaatc ttctttcctg ccattgactg catttgacta cattatagca ctttttgaat
    40081 aaaaatcata gcagggcttt gcaaaaccaa tggttctgca aagccctgcc ctaatctcga
    40141 atctcgtgtt tcttgtctga agaatgcgtc tatgacgcat tcttcagata cttatctatc
    40201 attgctgcag cttcctcacg ggtaatgaag ttcttgggcc tgaaggtatc gtccccataa
    40261 ccgcttaata cccccatatc tgcaagtatg tccagatagt tgattgcgta ttttgccacc
    40321 tggttcctgt ctgtgaaata tactctctta ggcttaattc cgtctcccag cattctcccg
    40381 aggatggcac aggcttcttc acgggttatc tgctcctttg gaccaaacag cctcttgtct
    40441 cctgcaatcc ttccatttac ccatccgttt gcataggctg cctttatgtg ggaaagagcc
    40501 caatcgggta tctccttttt atccgcaaag ggcagttgga cactatcagc ttcggtatcc
    40561 cctgcttcca agcctgctga aagcaatttt ataaactcag ctcttgtaat atagttgcca
    40621 ggcatgtaat agtatttctc acctactttt ataccttcta ctatattcat atgaacaaga
    40681 gagtatatca catcccttga ccacctggca tcgtccacgt cgtccaacag tctcaaactg
    40741 ctgttttcca gcactgcgta cttgctgaag cttgacagtc ttactgttat gatacccttt
    40801 tccttgtccg gagtgcctcc tatatagtcc catccgccct gctgctcatc aagcctgtat
    40861 actgcaatcc ttctgaggtc tgcggcctga tccgcctcat acctgaagct tacacttaca
    40921 ggagttcccg tactcgcacc cttgttgagg cttatatcat aaacgggact taaaagcttc
    40981 atgcccggga actttttcat aagctcttcc tgattgtcat acctgctgat atttatgtca
    41041 gcagaattta ctgcaaaacc ggccggtatg tcaagggtca gatcgtcatc tgcagtgagt
    41101 tttgtttctg ttcccggctg cagtctggct gtgtagatat cctttttgcc cttttcagcc
    41161 tcgattatcc tttcgagtac gccggcaggg cttgaagacc ttctgcttgc tgttatgggc
    41221 ttatttccca tgctttccac tacattgaca accacggcat cggtgtgaga ataatcttcc
    41281 gttactttat actccgccat aatatacgcc ttaccaggct tcagagcttt tatcctggct
    41341 ccgttttcaa gaactgcggt tccggtatcg gccatcaggc tccattttac aagttccggc
    41401 tttatgctca ggtgttttcc ctccgaatca attgcattca ggcttagctc caagctttct
    41461 ccaagttcaa cggtagctcc tccgggggtg atcgccaccc tctgtatgtc tttcagcctg
    41521 tcattatata ccgtaccgct gtattcgctg atattcccta tggggtcctc cgcaactgcg
    41581 aaaatttcca tgctcatatt atcacccata tccagggtat attcaaataa tccattctcg
    41641 tccgataaca cattgccgcc gttaatagtg agcttgcttc ccggctcgca ttttcccttt
    41701 attaatgcgg atgcagcgcc gttgctctgc gtgacctctg tggaattaat aagcagcagc
    41761 ggcggtatgg tatcgcatat tatccttatt actttatcgg tgaagtctcc ggagctgttt
    41821 tcagccctta tttcaaccat gttctcccct tcgttcaggg caagctgctg cagatgtctc
    41881 accgccgggt taaaggagta cactgtttct ttgttcaaaa ctatatttgc gccgacagcc
    41941 gtatccgcct ccaccgacag cattgcttcc ttccctgtgg aatagtattc gtctataccc
    42001 gactctgccg tattaagagg aagcatgctc ccttcccggg ccagcgaaag ctttagtgcc
    42061 ggcggatgcg gggccggaag aaatacagtg tccgacaaaa ttgcttcact ataatgctgc
    42121 ctgggtatgt cttctccttc aaccagatcc tcttccctgt gggcaattgc cgaaatctta
    42181 tagctcttgc ctgtttcaag cctcagggta gttccgtcct gctgagtgac ttctccgcct
    42241 attatcgcct cggttcttcc ggatacgtag gactccggat aaccctgcac cgatgaaccg
    42301 tcatcattca gaacagtcaa tatatagcct tgggccgggt atctgacctc agttccttct
    42361 tcccattcca ccttcaggca gccgttgcct gccgcggtca ccttcagatt cctgggcttg
    42421 ctcggagcct tgagatcggt taccgtaaac ggccctgtgg ttctgctggt atacccgtaa
    42481 tcctccttgg aaagctctgc ccgtatcaca tagctgccgg tcattacatc ctccggaagc
    42541 gtcacggtat aggtccctgc tgctgcgtca atatcctgaa ctaacagtct tcccgcttcg
    42601 ctgcttcctt cctcaagcag gtaaagattt acctttgccc cgtccagata ctttcctgac
    42661 caatcaacct taacactgcc ggttcctatt ttctcagcgt ccaaggacgt atattccgga
    42721 gccattttta tatcgtacag ctgtgctgcc gtaaggggct tatccgatac tatcgtccac
    42781 atacccggct gaggccccac aacggatatc cacagcttct gctcgtctct tccgctgtcg
    42841 ctgtccttcg cgggaatggt ctgatatctc atatttccgt ttatgtcgtc ctccaccagc
    42901 atatactctg tcccatccgg cctgtagacc ctcacattgg gctttgtgcc ttcatattct
    42961 atttcgaaca gagcggcttc cctgctctgg acattcaacc ggtatgtggt gtcatccagt
    43021 gcagtgacga cgggcatatc catgaataaa aatctcctgt cctcccctgc tgccgccaaa
    43081 tatatgctgt ccttcgcata caccctttcc ggctgtcttg aagaacccac aagcttcata
    43141 ttggtgccga atatcatcct gcctacctcg ccggttcctt catccctgta atttattgca
    43201 taaagcccct cagcaccatc cagggatgca attaaccctt ccggcagttt tccattctga
    43261 cctacatcaa actctacctt ttcttcgcca aagagatacg ttacccccat ggtcacacct
    43321 attactgtaa gcttccccca cagcttctcc gtatctatcc ccgcttcagc tcccgcaatg
    43381 gtcaatcccc ctactaccgg aactccatta ggtacaaaga gccttatcga agccatgcca
    43441 atgaagctgt ccttgtatat gttgagcagc acctttcctt cgatgcagct gaaggcctct
    43501 atggtggcag atgcgtggaa gcctatgggg ttgtaccatc tgagcctcag ggtggcattt
    43561 tttattatat catagccttt tatccctata tttcccttta attcggcatg ctgccaggag
    43621 acactaaggg tcaccttgtc catctttagt atcctgccga tttccgcaga tgctgtcagc
    43681 attatggtaa gaggcggagc tgagctatcc cagttcacaa ggttggaaat attatctatt
    43741 ccgccgccca ctccatacag ggtagccacg ggtatcgcgg gtattatatc cactcccacg
    43801 tcggttccta catataaata taatttatcc gggaagggat agggcttgag tatgaactct
    43861 atctctccgt ttacctttat aaccttaaga tccacatcca gagctaatcc cacttcgccg
    43921 ggatcgctga aggtatccac cttcagggtg gcttcagctc ctatatccac aggaggcggg
    43981 aaaaaatcct tcgggaagcc tacggtggct tgggcggcta ttcccttgaa ccctattgtg
    44041 ttatccggct tctgtcccat agccaccgta tccaccttgg cttctatctt gaaaggatct
    44101 ttcttttcat ctttcttgtc ttcattagtg ataatattgt cgttgttgtt attgttattg
    44161 ttttcgtctt cgtccccgcc accacttaaa gaaagcttca tggagccccc gaatatgaga
    44221 ccatttccgt ctgtatcctt ataaaagtat gcggtattaa acttcaaatt gaacccggca
    44281 agtatgtttt gcagacctcc tgccgcaccg gtcagagcta tttctatctt tttgtttaca
    44341 aggtcaccat catcaggagg tttcagagta tattggctgc ctttctttaa gttgatctca
    44401 aaatcccatt tccagaaggt aatggaaccg gataccgaaa ggcctccgtt tcctgccagc
    44461 tttaccgacc cattgctttc gcttatgctt atcggcgtcg tgctgttgta aagcagtact
    44521 tcgttcatct ccacaggcgt ttcggttctg gaataggcct catatatccc cgcttccttc
    44581 tgctgtatgt ctccccttac aatcagcagc agtttgtttt tgacgctatc cggctgcttt
    44641 tttaaatcac cttcattatt aaaaacctta atcttgtatg ttttgttgcc ttcgtcactg
    44701 accgcaagta taccgtattt ccggctcatg tattgattat ccgtagagat atttaacccc
    44761 ttctcaaagc tgcacccttg gaaatcctta tgctccagct tcaccgtata ggttccggca
    44821 tcacttatat ctctcagcat atctcccaga gttacctgta tcgttgtatc ccccgccagg
    44881 tttatctgct tgtggtttat attgtatacc cttgggttct gtccttcctt tatcagcttc
    44941 agctcccacc tgctcctgtc tttcatcaga ccgaagcccg ttccgttaat attgaaggat
    45001 ttccgcgaat cctcatagta caggttttgg ggagttattc cggtatattg tatatccggc
    45061 actttgcctg agcttcccgg cagtatcaca tatctgccgt atgcctgggg ctcataaagg
    45121 gaatcggaat ggagatatac agttgcctgg cctatcctgt aggtctcggc aaacaccccc
    45181 tttaccttcc agaccaggtt tgccctggaa tccctctgca gcagtcccag gtttatctcc
    45241 ttagactgcc ccggggccag ttccaggccg cctgaaagct ccagcacagc cctgacattc
    45301 tccatgggca ccgaaacagg aaggccgttg tccagtgaca gggatatctc gaattcctcc
    45361 tgtatatacc ctgttttgtc ctctttaagc tcaaggctgt cgggcccgct catgtttgcc
    45421 atgaaggtat gcccgtctgc tgtggcaaat actcccagac catagaaggt ttcatacacc
    45481 ctggtttccc ccggggccag cacctccgga tcccaataca gggctaccgc actgtcgctc
    45541 ttattgtgct tattgaaagg ggaagtaaat ttcacggtgc tgtccggctg ataattccat
    45601 ttggtggagc ctatacctcc ccaatgagcg acggtcatcc tgtccggggt ttcgttttcc
    45661 cagccgctta tcaatccgta ggacaccacc gaagggctta tatccgcatc cgccgactgc
    45721 cagaaggcgg gtacttcctc tccttcaagg cttatttcat gctcaaccgg cttgtcaatt
    45781 ccgggtatta ttataggaga accgtcattt gaccccagct ttgtgtcgaa cagcagcctt
    45841 gagcccacgc ttttttctgt cttcccctta ttggataccc tataggtaac ccttacgtta
    45901 cccaaatcag gatttccact gtctgagacc aaggtcagcc gctgctccac ctccagatcc
    45961 ccgagggtcc agagggaagt atttaccatt tggtcggttt tcggagcttt caccatgccg
    46021 ccctttattc ccagaaaacc gtagctgttc ccgaatatgt aatcctctcc gtctatgcgg
    46081 aaagtagtaa aggaggtgtc gggctttgac ccttcaaaaa gcagcggcgc atcctgatcc
    46141 ccgctcctcc ttggagcgcc ttcaacggtg cttattccga accttccggt attcttatcc
    46201 accacatagc tcacaaactg attatgaacc atcagggtat tctcttccgc tgccgcatat
    46261 acaagctgag taaaaccggt ggatgaaata aagaacgcaa gaatcagcgc aaaggcgatg
    46321 gttcttttca gtattctcat tactgttcac cccccaaatc ggttattaat acattaatca
    46381 gcttgctgtt cctctcattg tcatatacgt acagtgtcag ccagcccttc ttgctcacgg
    46441 gtatattatc aagagaccct gctgtaccgt cggtcttaaa gtaggaggca ttcctgaagc
    46501 cctctgccaa tttgaggctg acctgcccca caaagccctc tgcagtcacc tgcagcttgc
    46561 ttgctgcaag aggtataggt tccccttcgt acctaactcc gtttatccgg actatctgtt
    46621 cctcgtcact tctgaccttc acagtgagag tttttaccgc ctcatttccc acactgttca
    46681 caacactgta ggttacggtg tatatcccag gcttcagggt gtttacagca ccggttattt
    46741 ttaccttgtc tttcacatct ccgtctatac tgtcaacagc cgtaaaattc atcaggtcta
    46801 tggaacccct ccggttaatc tctatataat ccgggtatcc cagggtaatc accggagcct
    46861 ccaggatgat attcttcacc tctaacctca gagcctttat ctgtcccagg ctgtccctta
    46921 ttacgaagat gtactgcccg ttctgctggg ctgtaagctc tttgctgcct ccgttgttaa
    46981 ggaccgtaaa ctctttatcg gaggtgagag tgattgttac cggatttttt gacagttcgg
    47041 cagtgctgaa gtttatcttg acatcccctg cccccggctc gtcaaggtaa gccgccgaga
    47101 ctgttacaac cacctcgttc cccagttcgt ccagggctct catggtatat cgctccttat
    47161 ccgtgcaagg gtacacctgg ctgagagtgc ttccctccgc cggggttatc agttcatctg
    47221 accttactat agcggtaata gtgtttttgt aagaatcata aatataatcc agccaaagct
    47281 tcggaggaat cctgtctatg ttctccacag aaggctttac tactttttct atgccgtact
    47341 catttacaaa agtgaaattc caactgccgt ttacttcggt gatgaaagta tagctgtcat
    47401 tttcctgcac tgtttgaata tcgccgcttt ttaactctga tcccggctcc ggcgtcatcg
    47461 tcactttcac cgcttccctg gtgggatata cggaactgta agagaccgcc acccttgggc
    47521 cttctgccgg tatagcggtt aactgtgcaa gggctctgcc ttgatttccc gcttcatctt
    47581 cgaattcaaa cacgaactgg ccgttgtttt taaacacata tcttccgctt ccgccgttgt
    47641 ttattatagt aacatttccc ctgctgtcgt ccgaaagctt caggttagcc gtaacaggcc
    47701 ctttcttggg atcagcccat gtgtagctta cccgtcccga aggaggggta agatcatcca
    47761 cagatgagct gtccgatgct ttaagcatac cggagtattt acctgcggat gccagcaatg
    47821 accccggggc atatgcccgc ctgcttccct ccgtcatggc aaccgtcaca ttatccaccc
    47881 agtcttcatt tacaatgtgg cctacttgga cagtgtataa attgccccaa gccatgtata
    47941 aaaaatatcc gttcacatca aaagttatct cggctttatc tacttccaga tagtaatatt
    48001 catctgacgc aagctcttcc agcaaatcgg cattaagata ttcagaatta attgtcctta
    48061 tagttatatc ctccggaaag gctgactgca tcacacgacc ttctattata tttatattgt
    48121 cgttatctat caaatattgg ttcagcgcgc cttccagagt ttcccaatcg caatcaagtc
    48181 tttcgggcat gaactccata ggatagccat cgtttgcctc aatagtcact gttacaggcc
    48241 ccgaagtcac ttcatcgtag ggcgacattg ttactgtgcc tttacattca agcatatctg
    48301 ggtcaatttc agactcccag gcggcggtta tcaaccaatc ctccgcatcg ggtccgtcgg
    48361 agtcataggt atagcggaag gtatactctt cacctgctct caggtcatat accgcttcac
    48421 ttaccgttat gggctcaggt gaaacaatga aggctctcat aactccctca tgctcccctg
    48481 agtatacaat atcaggctcg gtatattcta aaaccatatt atacggattt gacgtaaatt
    48541 cgccagtatt ccctgaatca tcagttgcct ttacatgcaa gtaataggtt ccgctcactc
    48601 cggaaaagct tacggtatta ccattgggat tgtagtcatc cataggtatc tccgtccaat
    48661 ctgtaacatc ctctatattg ctttcagtag taaaggcgta ctcaattctt gctatgtttt
    48721 tattgtcgct gacttttatt ttaggtgaaa cactcattgc attgctatga ttgccgttgg
    48781 gagtgactac aatcgttggc gattcggtgt ccggaggctc cggttccggc tcatcatcat
    48841 cgtcatcatc gtcaacaacc ggctgaatta ccacaggtat aggttccaca catgccttta
    48901 ccgctccctt attcccggcc tcatcctcca gtataaagta atgaggttct cccctggcaa
    48961 tgatttcctt gtagtatccg ggaacatatc caattacctt tatatcctcg gaagggcaca
    49021 agttgtcact gatgtcaaca agttccgcag taaagaaata gttcgaacca tagtagctgc
    49081 tgtacttgac ccttgccgta ggaggagtta catcccttat aaccgtagat gcagctgcat
    49141 ccgactcttt ccctgcacta tccctgtatt ttgtatagaa ggttatgggc ccttccttgt
    49201 caggcaaagg aatacaaggg ctgtaaccct gggtttcaaa ggaagtagcc cctttttcaa
    49261 ggggtatcca gccgctccag cttactccgt ccatggacca ggagaattcc tctacagagt
    49321 tgaaaatatg gagctctgca aaatcctcat tggtgtattt gcttcctctg taaaaattaa
    49381 tgtatggggc tgaggctgaa agctttgatt cagcattgcc gtatggcccg aaaagcatat
    49441 ttctgttgtt ctggtgcgcg tccgtcgccg ttacattcac ataggtatcc ggcttcagtt
    49501 catccttgcc tattattatt tccccgttct ccggaagctg ctgccggtcg gaattagtta
    49561 aaatgtactt tacttcgata tccttccggg gagtataatt atcggataca tggatcgtga
    49621 ccttcatgga tccgtccgcc tgcatttccg catttgcagt cacctgggga ggcttgaagt
    49681 cgaaaagcag cggctgcggg accgttttgc ttcctatagg cttcctttcc tcgtctgccg
    49741 caatgatgtg caaataataa tagccgtccc tctcccgggg atctccttca aaatcttccg
    49801 aggtattgat cctgtggctc tccttgtacc tccatatccc tgagttctct gaaaactcag
    49861 gcattacagg ttcccgggtc atgcagtacc agagcccctt gcagcgccaa ttcacgccgt
    49921 cttcccttac ccagtctgca gagcataaat agacccttcc ttcagcggcg ccccccaggt
    49981 tagtctcaag actgacgctc atttcttttt gagtctcaat ggaggatatg tcaatatccg
    50041 ttctggtcac atttatgtcc gtcgcccagc cgtatcctat atatccatgg gtcgagccgt
    50101 agaacatatt gccctttttg tcctttgcat gtacaaataa cctgtagtta cttgttctgc
    50161 ccggatcgat cccgaacatt ccggccaact cgtgggaatg gtaccatctg cccggagcca
    50221 gggatttcgc agctctcata atacctccga agtccgcgtt ggtataccca atgccgaact
    50281 tcaaggtatc ataatccact cccgaaacct catccgtaac atttatcttt acgtaaaagc
    50341 ctcccttttc cttttccgct tccttggtgt ggacggtaat taccggaggc tcggtatctc
    50401 ccacatatct tttactatcc gaaagcttca ctcctcctat gggaagcagc ggcgccttgt
    50461 tccctgctgc atccatcaca tacagctccc cataggaaac tcgttctgtc gacccgcttt
    50521 ccttccactt gctgtatata ctccctgtta tctcaagggg atcgaagctg ttctccatgt
    50581 tgtcgaaacg aaattccagc attgttgtgc cgttcccact cgcatagtat gcatcagggg
    50641 taccggagcg gtacggatcc aatgcccctg tccagtcccg gttcaatgcg ttcagataca
    50701 gcttcgcatc gtagcttgaa aatctgcctg ccctctgata gttctcaaac tccagcttgt
    50761 ttatgttatg tttgataaag gctccatcgg atatcgttat atatacaggc tccgaaaatt
    50821 tgaaatacaa aggcaatgtt tgagaacggt ctaacgttcc ctcgggcagt ccgccgttta
    50881 tctccagcag taccggagct gtcgtttcca ctctgaaatc ccgggtatag atcctgtaga
    50941 cattgctgac tccgcttgcg gagcctgagg caaaggatat gatcctgtat tcattgggca
    51001 tcgggttgaa agcattgtct gtgatgactc ccccctccag caccatttca tatatgtctg
    51061 aggctctgaa ctcatctccc ggccgcacgg tatatttgaa ctttatctgc ttgctgtaat
    51121 catttccgta ggtctcctta ttatacctgg atttgtcaac cgggtaggca tatccctcca
    51181 tatcccttcc gtcggcgttc ctgtacttga acttcatctt cagctcgccg aattgctgca
    51241 atatccccct tccgttgttg tcccgcacat gctccaacag cagcttttca tcagggtaat
    51301 ttggggacag ctgggcattt agaaatgaat tcctctcggt ggcgttcaag cccctcacat
    51361 aagccagata acccggatcg tttatgaata tgggttcgtt aaactcaagg tcccaatata
    51421 catcgtcccc tatgccgtag taatccttca tccttctttc ctcgggagtc cccagttccc
    51481 gcctttctcc gaattccaca tatttgggta taggggccac atcatcagtt atatatacct
    51541 ctatattcct gaaatatatt tcacatttgt tatcatcaag tatactgatg cccttctccc
    51601 cggcaagggt cagctgcata ccggttgcac cctccggcac atagcccgaa aagcttacat
    51661 cattccagcc gctgaaatca tgctgcatcc gttccaccgt tattgaaccg cttctcccgt
    51721 cacccaacgg agcccagaag tccaggaata tactagccac atctccatcg aaagcctgtc
    51781 cgtaagcctg cagccttacc ttgtatttca gcccggcttg attgtcggcg cccttaaggg
    51841 gcatggtcca aaagtattta aaactattat ttatattttt aggcttgatt tcatcccctg
    51901 tctcgggatg ggactcaata taaaagctgt ttgtctcgtt tgtgctccat tgtcccttgt
    51961 caggataaca gttttttatc cccttggatg caaaatgtgc tccgttgtca aagcgcacat
    52021 cggtacccga ccccgcaacc gggacaatct gcgatgtaag caataccaat gccataatta
    52081 ccgatatcat acctttgact gtcaagtttc gcataaaacc accctcttac atttataatt
    52141 tcatactcag ccaagcagaa taacatccat aagctctcct acggtaagcc gtacccttcc
    52201 gttcccccat aggacataat cgccgtcagt tacggcggtc ctgaaaaatg cttcatccgt
    52261 aagttcggcg tatttcgccg tctgcagctt accggacagg tcaacgatta ccgtactgcc
    52321 tccctccatt tccatgaaca gccgatagtc cttcatgggc agtacggttc ttatatagct
    52381 cataagcaat cctccttccg gaattcaata gaaatatgca aaaaaatcct atcttactaa
    52441 tatattgtaa gacaggattt tttatcccgc atcgtccaaa ccgacgatat ttttaattta
    52501 tttgaaattc ttattcagcc ctgcacattc ctgaatacag ttcgataaaa gcagcactgc
    52561 cccccagcag tgtgcagaac agcaccgggc gccggaacaa gcggttaaat aacctattac
    52621 cctatttggg gcatcaagta tcccggcagt ttttgtccgt ctgaggcttg cccggttgaa
    52681 aaaggcggaa acaataagct tttatctcct tgtccataaa actcctccta catcacttca
    52741 gcttttccgc aagctttgtc cggcggtcta tgtccagctt ctggaatata gcgcgcatat
    52801 gagttctgac ggtgctttcc gtaaccacca gacgctttgc tatctcacta ttatgcagtc
    52861 cctccgccgc cagctttgcc acctcatatt cccggggcgt caggtcagga ggaaattcct
    52921 ccggtgttat atcttcatgg agccttgtcc agcccgatga gaacctgtct tttatttcgt
    52981 taaacttatc ggtaagttca gggtatttct ttctgaccag ttcatcggac aaaccctgaa
    53041 gcagccagga gtatgcagca aagggaaata tcagtccgtc aggcagagcc atttccccgg
    53101 cccgtgcaac aagggcacat gcctgatcac ggttacccac cgataaatac cctacagcca
    53161 gagtcagata caggatcatg tctctgaagg gattgggtcc caacctctcc ggatacgtgg
    53221 cttcaagcat tcccagcatc ctcgggaatt ctcccctgtg catcagatag cttatatgca
    53281 cgaaccctgc attggaaatc atcaatggag tgagcctctt atccgaaaag tccccctttt
    53341 taagccaatc ggctattctc tcaagctgat gcagttcgtt aaaaagcacc ccccgggcta
    53401 tatccagggc ttgcctgagt atgaaggaat cctgagaggt aaaggatgcc gcccgctcca
    53461 tggagctgat ggcattctgc cagcctgccg tatcggcttt gtgcagtgct atctcggcca
    53521 actgtatcgt agaccccaac agcacaatgc tttgctgcct gctctcagcc agaaaagcag
    53581 ccttgtatgc aagtatctca gcttcactca ggtttcccct gtgataggcc agctccgccc
    53641 ggaacagtac atccgctccg ctgccgtggc ctcccgtaag ctgtgaatac aacccgatat
    53701 actcctccag ttcctccgct tccctatcga cttctcccgg cacagtatgc agttccgcca
    53761 gggtgcagta atggcccagg caccatgggg ctgaaggcag tataacccgg gaacatctgc
    53821 ctttgaaaag caccgccgcc tgcctgagtg ctgcggtcat ttcggcaatt ttcggatagc
    53881 ttctgtagga agcaagaagc agccattccc ccatgaggtc ggaatccccc gacagctccg
    53941 gcagttctct cagttcctcc agcagcagct catactgagc ctttttccca ttcatgtaca
    54001 gtgcccaggc aactctgagc attgatagca tattatttct ttttatatca atcgggcagt
    54061 ttgcagctat atccagagcc agttcagcaa aatgcgaatc gcctatattt tctaaaatat
    54121 tattggaaaa atccatggaa agcatacgct caaaatcctc ggctttccaa taaaagccca
    54181 gggcgcttga gattttgccc tcatccctgc ataaatcccc ggcggacaga aaacacttcc
    54241 gttcaaaagc acttccgcgt tcactggact tctgaataag cagctggctc agaatgctgt
    54301 gaagctcata tcgtccttca taagcctcat gacggataaa tgggctttca agggcatcca
    54361 gagcgtattc cggcagctta tcgaagccgt tcagggcaca ggcctgctgt acggtaatca
    54421 tctcaaaggg agacagccgc agaagaaata cctgctgctc ttcggtcagg gtatcccata
    54481 caagatgctc cataagagcc agtataccgg acataccgta taaaactccc gtatccagat
    54541 aagcacagag ctgcaaatat actgctatga tccagccctc agtgtaatgc tcaacagcca
    54601 gggcatcctc atgagtgatt cttaaacccg caagggcata gtaacgtctt atatcctctg
    54661 catccagctt caggtcggag gcagtgatat gaaggaatcc gcggcctgca attgccgtaa
    54721 gcatattgcg tttaagcagc tgggtcacaa ttatgatatg cagcccttct cccccgtgtt
    54781 ccaaaagtgc agtaaaaaaa gaagggggca ggaatgtatg cagtaattgg aagttgtcta
    54841 tcacaaggta ggcttcatgt ctgcattgta tgcttcggat tgcatcacaa gcctcccctg
    54901 cggtaacggc attgggaaat tcaattttta acagtcgttc tcctgcctgg ctgtctattt
    54961 tgtcaatttc atggcaaaga cgacggaatc ctgcggcagg agcttcatca acagcagtaa
    55021 accaataaac aggcgtaccc tgagaaagct ccgctttcaa gaaatccctt attgccgtgg
    55081 ttttgccata accggaggga gcttccacaa cagtaaccag cgcatgaaga atgtccttta
    55141 atttttgctt caggctgtcg gagaaataat gcagttctcc tctgctgatt ctgcttcttc
    55201 ccatatactc cctcctttga tggctattgt aattattata caaatattat gaaaaaatgg
    55261 caatatactg caaagttttg cacaatagaa gaataatgca acagaggaca cggggacgcg
    55321 gggacgcggg gacgcgggga cgtttcgcgt gtcccactgg aagtgggaca ggggaaacgt
    55381 ccccgtgtcc ccgtgtcccc atgtcctcag aaagcaagcg gggaatttct tttgtagctt
    55441 ttctgtctca atgtaaaaat gcaaagagtt aaaacccgaa tccgaatctg cgtcttcgtc
    55501 ttcgccttct gaagcgtctg agaagcagca ggaggagcac agtaccaagc aatcctctga
    55561 agccttgatt tccatagccc atatacctgc ctgacatatc gtatagtctg tagtcgttac
    55621 tataaaaccc tataggcctg ccatccattc cgtaaacaaa cctgtcgtct acatatgcca
    55681 tagggttgga atattcatcg taaatcatcc ggccatctgt ataacctgca acacgattcc
    55741 agccgtcata aatcacatca ccgtcaacgt atccctgaag cctgctttgt gaatcatata
    55801 catgctccaa aatctcaccc ccttttttat ttcgtttata taaattaaag aaacttcatt
    55861 cccctatcat tattatgtag catgtacatt ccttgacatc atcaaattgc atattgtatc
    55921 aaaataccgg atatggtatt ctataataga agtaagttgt gtgaatgtta tctgttaaag
    55981 gggagtaact gcctgataaa ggtgatttaa gtcaacaacc ggtagttggc acactgcctg
    56041 gcttaatcct ttgacgattc aaagagtaag acctttagca taattttatc cgtaatgata
    56101 aaattttgct aaaggtcttt tttaaacaca gcaagagagt gacaccccgc actcttttat
    56161 aaagaatgct gattatattt aataatattt atacgaacgg agcgtgaagc aaaatgtggt
    56221 tttcaaaatc accggaagaa gtactgaggg aatttgatgt cagccagtcc gtcggccttt
    56281 caggtgaaga agctttgaaa aggctggaaa agttcggtga gaacaagtta aaggggaaac
    56341 cgaaaaaaag cctgatttcc ttgtttctgg gacagcttag ggacatgctg atatacatac
    56401 ttctggcagc atcagtaatt accattctca taggcgaata tgtagatgcc gttatcattc
    56461 ttatggttgt catattgaat gcagtaatag gagttataca ggaatataag gccgaaaaag
    56521 caattgaagc acttcagaag atgactactc ccaaagccct tgtcaggcgt gacggtgaag
    56581 tgaaagaaat caactcagaa gaactggttc ccggcgatat agtcgttatt gacgccggaa
    56641 gatatattcc tgctgaccta aggcttctcg gaagcgtaaa tctgcaaata gaagaatcag
    56701 cacttacagg agagtctgtt cctgtagata agaatgcaaa tgctttattt gacaatccaa
    56761 aggctccctt gggtgatttg tcgaacatgg cattcatgtc cacactgtct acttacggca
    56821 gaggtgaggg tgttgtcgtt gctacggcca tggaaacaga gataggaaaa atagcaaaaa
    56881 tacttgacga agacagcgat gaaatgacac ctctccagaa aaggcttgat gaactgggaa
    56941 gaatacttgg ttttttgtgc ataggaatat gtgcccttat attcatagtt gcgcttatac
    57001 aaaaaagaga actttttgag atgttcctga cggcaataag ccttgcagtt gctgcaattc
    57061 cggaaggcct ggcggcaata gtggccatag tgctggctat tggggttaca cggatgtcca
    57121 aaataaatgc catagtcaaa aaactcccgg cagtggaaac tctcggttgt gttaatataa
    57181 tctgctccga taaaacagga actcttactc aaaacaaaat gacggttgtt aaacactata
    57241 ccctgaataa tttgaaggaa ttacccgccg cccagactgc tttcgaagca gttggggatg
    57301 aggcggaatt gatcaaatcc tttgttttat gttcggatgc cacctatgaa aacggcaagg
    57361 gtacaggaga ccctaccgaa atagccctgg ttgtaatggg tgataaatat aacctgtcga
    57421 agacaagctt gaatgaaaaa tttgaaagag tgggagaaaa gcccttcgac tcggacagaa
    57481 aactgatgtc aaccttgaat aaggaaggaa acggttacag agtgcataca aaaggtgcat
    57541 tggataatat actgaaaata tcagcaagtg ccctggtaaa cggaaagata gtaccattaa
    57601 ccgatggaat gaaagccgat tacatgaaag tcgccgaaga aatgtccgat gaggctttaa
    57661 gagtacttgg agcggcattt aaagataccg gctgtataat aacacctgag gaaatggaaa
    57721 aagacttgac ggtaataggc tttgtgggaa tgatagatcc tccgaggctg gaagtcaagg
    57781 attccataag ggaagcaaaa actgccggca ttacatcgat aatgataacc ggagaccata
    57841 aaaataccgc ggttgcaatt gcaaaggaac tggggattgc cgaatcaaga gagcaaagca
    57901 tgacaggctc ggaaatcgat gaactgtcag ttgaggaatt tgcagagaga ataggcagct
    57961 acagagtatt tgcaagagta tcgccggaac ataaggtaaa gatagtcaaa gcattcaaat
    58021 cccacggcaa cattgtatcc atgaccggtg acggcgttaa tgacgctcct tctctgaaat
    58081 atgcggatat cggtgttgca atgggcataa ccggaactga cgtggccaag ggtgccagtg
    58141 acatgatatt gactgatgac aacttcacga ccatagtcca cgcaattgaa gaaggcagaa
    58201 atatttataa caatatcaaa aaatccgtaa tattcctatt gtcctgcaat cttggagaag
    58261 ttatttcaat attcgcatcc atattgttct tctggccgat accgttactg ccaacccaga
    58321 ttctctggat gaatcttatt accgatacat taccggctat tgcacttggc gtcgatcccg
    58381 gagataagga tgtcatgaag aaaaaaccca gagattccaa agaaagcttc tttgcaagag
    58441 gcgcaggcgt aaaagcaatt accggcggtt cactcattgg tatacttact ttggcagcct
    58501 tttatttcgg gctccatgaa ttcggataca gcctgggttc aggtgaaata cccgaagaag
    58561 tactgactta tgccaggaca atgtcctttg ttgtaattgt tgcttcacag ctgttcttct
    58621 ctctttcaat gaggaatgca acgaaatcca tattccaaat tggattcttc acaaatatga
    58681 agctgatagg tgcaattatt gcaggtttta tcctgcagtt cggagtaata acaataccgg
    58741 ttttcgcaag ggcatttaaa gtccacacct taagcttgta tgattggagc ctggtaattg
    58801 catttgctct ggttccgttc ctggtaaacg agttattgaa agtattcatg aaagctgttt
    58861 ataaagagta atagctgaaa aactcttctg acacctgact tcggtcaggt gtttttttgc
    58921 ttataccgca atatatcata aataaatcag caatctgaat aatatctatt ggtctggcag
    58981 gttctatact gttattagaa aatatccgat taaactgtat agttggaggt gtaaatgtat
    59041 acaagcaagc aaaaactctt gtttcggtgc ttctcatctt tgctttgatg ttcgcgccga
    59101 tactgctgcc cccaatcagg cagccttggt tcaggatagc gggcccctca tagagcttga
    59161 cagcatgctt aatgagttta actcagaaaa actgcctttt gtaggggaca gaagcgggcc
    59221 cataggaaag tttacgaata caaaggcagt gcttaaggaa aatgttcata taactttacc
    59281 caatgcattg agtactatag caccggggaa gaaggcatcc tgttcaaccc tgatgctgtg
    59341 ctgaccatag aaggagcaag caagatcgtc cataatgcag tgtcaatcct tctgggtaat
    59401 gctcaacaat atgataaaaa agaaggaagc gggaaatagt ctgcttctta atattgcccg
    59461 ataagcatga gatgagttta ttgatataat cttttgtaag agagtgagat tatggaagac
    59521 tttttgctgg cactaaaacc aacttttcct gatgtgcaaa tgaataagtc ccaagtatat
    59581 ccatgcacaa atctggcggt catagaaacc tcctatataa tgttggagga atgcgcatgc
    59641 aatcaattcc attatataat ttccatgaaa cagtcaccct tggtcagaat ggagaataaa
    59701 ctgcatatat taaaaaaaaa acgttttccc atgcaatcct atgcagagcc atcgaataga
    59761 agatacgggc actatcgatt tcaaggcttt tattctctat ttcgaaaata catttcttga
    59821 aacaatcgcg gaagagctgt tcggcaatag aaatctggtg ctgaaaaata attgttttga
    59881 actcagtccg gagcttaggg agttgatata tatacatccg cgaatgccgt gcaaagcagc
    59941 cgggctgttc catgatgctt gatttgaaaa ttgagaaagc aaaagacttg ctgcaaagta
    60001 caggtttttc aatagagcaa atttgtgatt tatgcggctt cagcagtttg agctatttct
    60061 ctcagatttt caagaataaa acaagcctta cccctacaca gtttaaaggc caatataatc
    60121 attcatccaa gctgttcttc tttaaaggga aagaataacg gtttttgttc cggactactc
    60181 aatatcctta tccgcctgct tttcacttat tttaaataaa gcatgctggc aggcaatcca
    60241 tactgccaag aaaataattg cctgaagtac gagctttgat acatcctttc cattagccag
    60301 atcatagtcc attatcagta tctgaactgt tgaaaatact atggttcctg tcaatgcacc
    60361 tattaataag gtttttttct gtgtgcttct gctcctgtag gtatagtaaa acccctgcag
    60421 tatattgctg acaacaacat atatggaaat gccgacagta agaaggaata tatcggcata
    60481 ttccctgata tcctgatgaa gcatgaactg cctgtaaagc agtatgcccc ataatgccag
    60541 aaagcagatt ccgaatgcac ttgagtttat cttccttttg ccggctatga ttctttcatc
    60601 tataatcttt ctcataaact aatcctccca gaaaatatcg tctaaagtct tgttcagctc
    60661 tttgcatatt gctatgcaca gttttattgt aggattgtac ttgccggctt cgataagacc
    60721 tatagtttgc ctggtaaccc ctatcttatc tgccagctgc tcctgcgtca gatctctctc
    60781 tattcttgcc agtctcagct tattgttttt atctcccaac gtatcaccct cttaccgcga
    60841 ttgtaacata tatattgctt aatgtcaact atattttgca tttaccatca tgcctgcaca
    60901 ttgcctgtac agcttttctg cctgattgag cagctgtagt aatacctccc caggcttcca
    60961 cccattgccc cgccatatac agaccttgta tgtccttata agcatatctc ggcctgcttt
    61021 gtaaaagagc ccttataata cttgaatccg gtttccaggc ctgcatggca cccatccagt
    61081 tcccggtata acgctctcgt gtaaggggtg ttgtgacatc tatcacttct atcttatctt
    61141 taaagccggg gcggtaacga tccattatat cgataacagc ttcagcacag cgatctttct
    61201 ctcttttata gcgggcttta tccgaagcaa aatctttcca ccaaagataa tccgaatcca
    61261 aaaaaactgt aatggcactc ttacctttag gggctgcatc cggatcaaag catttattgt
    61321 gtacagtgat tctaccgtgc tcccgaccag ctgcattgaa actgtcaggc agtatatatg
    61381 taacaggccc ttcttcagcg gaaaaatcca tgtcaacacc tatattaact tgtaccggct
    61441 gatccgaaac agcatttgga ttaaaggttt gttttgtatc tccctcaggc tttccaagca
    61501 gtaacgactg agaaaagcgg ccatcaacag cagaaaggac cctgtccgca aaatgtctgg
    61561 agccatctgc aagaataata ccttttacgg ccccattttc tataataagc ctttccacct
    61621 ttgattgata ttgtatactt ccacccagtt ctatgaacct tctttctatg gcttgtgcaa
    61681 aggaaagcca tcctttaagg ggtattcctg ccattttcct atgtgcatac gcaataggaa
    61741 gcagaacccg caagcccggc tacactccca tcaaataggt acccttttct ttcccatgat
    61801 gtacatagtc ctcccggttt atcccccatt tcaaagatgg ttgcccgata cccgttcatt
    61861 cttgcataac acccggaagt aaggccggca actccgcctc caataattaa ttatctgcat
    61921 ttatctggat gatacaggca tacttgcata tttcttaaga agcctcaaat aatggtttgc
    61981 ctctctgact acatgatcgc ccaaaagcgg ataggcaatc gatctgatag tacagttaat
    62041 caaaccttgt gtcccttgcc ttttaaaatc cctgatgtct tttgcagcat ccgaggtttc
    62101 cgttgttacc ttcgcgaaca agtttaaatt atcttccagt ttctctgcct cggcagtcaa
    62161 catatcaaat ctattgccaa aatcgtttgc agtatcaaaa agcttcactt ctgttgggtc
    62221 cagcaggcct cttatgaatt ttgaatgctc cgccattatt ctgttccaga atatctcctc
    62281 tttaatagca tcatctattg tatttgatga atttttccgc tgcaatcgca tcaagcttct
    62341 tatataaaat tttgcttctc tcagaatatg atccagaagc aatgggtaat tattagtaaa
    62401 tagtctgcag gctaaaacat cgcggagcaa ccttgctttg taatttgcca atgcgcgggt
    62461 cgaagctaag gcttgctggt tcagcatata aacatcctgc agcaaaaccg gatcaatact
    62521 gggttcctcc tcatttgcga aggctaattc cgctttggta attctgctgt taatacttat
    62581 ccctgtatag aattcggttt ccttctccgc ctccagggtt aggtccgtaa ccaattcccc
    62641 ggaaccggtt acatcttcac taagtatacc atccgaaagc tcaattgcct ttatcagaag
    62701 attgctgaaa acaattttga aagcctcagc ctcttctgcc ggttctctat tttttgcagt
    62761 aaaagccgct tctataaata ttgagtgctc ttttgctatt ctcataaaaa ataagtttaa
    62821 ctccagtgat tctcttatat attcaaaatt agacagcata tattaccaac tcctttaaaa
    62881 ataagtattc acgatatata tattcatgcg acgcgcgcat tagtaatgaa ctcaatataa
    62941 tttaaaaggc aggcacttta gagtacctac cttaaattgg ttaatatatg cagtttttta
    63001 gtacagataa caatattgcc gcattaactg tgtcttcttt cttcttgatt gattcttaag
    63061 tttttgaagt gactctttca tacagtttgt ttctttaaaa gttcattagg aactatacta
    63121 aatcctaaag cattttcaaa aagccgcagt atgcctaatg aagtttggta gttttcttcc
    63181 aacgcctgca atgccccggg tttatcgtga aattccctcc gcttgttctc aaagcgctgc
    63241 tccagagttg caatttctcc gcctataaaa aatttatccg caaggtatac aatagtaacc
    63301 tcagatattt tgtcttcctg ctccggtaac agcttcatgt gctgactcac aatttcggca
    63361 agaagaggat gcccgatttt ttccaagtgc tcagctccgg cacatgcatg atttggctgc
    63421 gcccttgata tatcgtgaag caaggcggca agctcaatcc ggtgcaaatc aagtgagtat
    63481 ccgaggctga ttagtttttc tcccaagctt actgccaact gtgcaaccaa agccgagtgt
    63541 ctgcggatat cctccggcac ggtatcatct gccagcagct ttattagctc atagtcccgg
    63601 gggctgtctg cgtccagtgt tatcccaatg tccggaagct ccattgcttc ttttggcccc
    63661 ttataatttt cgataacccc cctcaagccg ttctccccgt cataagaaag aacggcagga
    63721 acaacactgc ttttaattaa tattggatgt ccctgccttc cgcatctttg cggcaataag
    63781 atatcgcagc cgcttatact catatgttcc atcattgcgt caaggctttg cttcgaaaaa
    63841 agcggtacgt ccccggggag aaaaaagaat tgttcggcct tgtcctgaat atactcaaaa
    63901 cccatacaag ccgaataaaa catatctgta cgctcatagt tcccattata aaggcatatc
    63961 acatcaaatc cggtcaaata ttccttcaaa cgttccgcaa acttgcctga tatgacaaca
    64021 attggtgata ctcctgccga tttcagggta ttgattaccc tgccgattaa tgttgtaccg
    64081 gcaagcggaa gcagcggctt aaagtcattc atccttgagg acattcccgc cgctgctatc
    64141 accgctccgc attttatttt actattcaaa ctactctttg tatgagcagc aataatccgc
    64201 cactgtcttt aaaatcagtt tgaatttgga gttggcagga acaataaagc tatcgccttc
    64261 tttataggtt ttgaatactt tttcaccggg caggcaaaca tccatctcac cgccaagaac
    64321 ctccataagt tcctcagcgt ttgtgccgaa ttcatattcg ccggggagca tgacccccag
    64381 agtcttcctc tctccgtttg cggtaaatat tgttctgctg cttaccttcc cgtcgaaata
    64441 gatattggcc ttttttacga tagtcgcatt tttgaattcc attttttacc ctcctttaat
    64501 tagttattat ttttatatta atactattcc aggcaaatgt tgcgtttccc tgcgcctgta
    64561 aaaggaacaa cagtgccgta ttccaccttt atatcacagg gcagtttttc cctcaaaagc
    64621 tcagcatcca attgggattt tgtgtctgct gtaaggagaa gtctgttgcg gcccacaagc
    64681 cttgcgctgt aattcctgag accgggaatt gcaaacatga tttcgtccaa atcatgaatg
    64741 ttgattgatt tcccattctc cagacggatg atattggcgc tgcgccccct gaccttgtcc
    64801 aaccgcggta atataccccc acacgggcat ggttttgtca gtattcttgc gatatctccc
    64861 gtgcggtatc tgattaaagg catagcttcg tttcgcagtg tagtaagtac cacttccccc
    64921 aattttcccg cattcaactg cttgcccgtt atgctgtcta caatttcaat aagcaggtct
    64981 gcatccctta aatgatatcc ttcacccgcc tcacactgca caccgccgcc gaatcccgtt
    65041 tctgtcatgc cgtaatgagt aaacactttg caatgccatg tttcttcaag gtatctaatg
    65101 acgctctccg gtacatagtc tgcacttaac agtacacttt caggccggag tctgtcatct
    65161 gtacggcaca gatacatgat ttccgccggt acacctacaa ggcagtcgta acctttcgcc
    65221 gcctccaatg ctgccgctgc gtcctttaca ttgccgtgaa tatcagagga gactccgatc
    65281 cgtttcaggg ctttctcaag caaatcggca atactgttct ccgtccggct ggacatcatt
    65341 attaaaacag attgcccgct gtgaaccatg gttgacatgc cgcaggcgaa gaaatctatt
    65401 gtccgctcca ggtcattttc actaaaatat atccttttgg ggcttccctg gctgccagaa
    65461 gtggaaagtg aggtaatccg cgtaatctcc cgggttggta cgcaaacaaa ggcttttggg
    65521 tcttcattga tatcttcagg ataagtaaag ggtatatcct gcatatcccg taaagttttg
    65581 ataccggata tatcgattcc cttcaacttc ttgcaataat aatcgcagtt attacgggca
    65641 tattcaacgg cttttcttac cttatccaat tgccaggcca tcagtgtctg cagcgaaagc
    65701 ttttccttca ggccggtgcg ttccgctgcc cattgctgaa ccggtgaaat caaagtcatg
    65761 cccccttttg agctattata aggcaatagc cgcattttat ttctttaagc ttcccgctgt
    65821 cagtccccag gttttcacat aatgcttttg cgccatgttc aaaaatcaac tgcccccaca
    65881 tcctgtgaag ctcatcactg tagtcttcaa aaagcttaat atcaaacccg ctcattatga
    65941 gcttatccag gatttcctct tttgcttcta tgcggcccaa cagacccgaa agcgctgcac
    66001 ttgaacctct ggcatacata tcggatatta ttagataccc tccgggcttc aatactcgct
    66061 gacactcagt caaaacaagc tccggattat ccatttttga gaagctgcac tcaaaaagaa
    66121 gcccatcaag ttcctcgctg tcaaaaggca attccgtact gtccccggca taaacaaatt
    66181 gagtcaccgc cagcttcact gcatccttat ccttttcgat tccgcagata tcatagtcgt
    66241 aattccgtcg gatatgccgc actgtggcac ccagcccgca gcctatatcc gcaagctttg
    66301 catccttcga aaaaccacaa aattccaagg ctctttctgt tattttatag cccccggggc
    66361 ggcagatatc accggtacaa atattcttca tgtcatatcc catccagtca ggtaatatgc
    66421 gcaaaaaggc acgaatcttc cattatcaaa aacatgcagg ctgcacctgc gcagccgttc
    66481 caaatccata ttcccggcat cctggaatgc catggctgta attgtgaagc catgggattt
    66541 tacacgcttc aggaaataat ccatatcatg aatatccggc ttgctgcagc aggaatgcga
    66601 ttcctgctcc tgcacctgcc tctcccagcg ccttgctaca aaagccctgt ttttatcggc
    66661 ggataccggt tcgcttccgc agcagtcccc cttggaggta tatttaccgg aaagcggctc
    66721 aattccatga ggcataacca caaagtcccc atgaaagccg caaagggggt ggtcgcacct
    66781 ggagggtatc aggttttttg ctttgatctg cccctttgtc tgcacttcaa tatcatatat
    66841 aagctcatca agagtgtatc ttacctcatc cacaggcctt tcgggtattc gtccgaaata
    66901 acttaccggc tgaaaatgaa cacctctgac agcaggtgat tctgcaatgg caaaacgcag
    66961 cagttcccct atgttatcgg tattgacacc gggaaccata gtcggtacaa gggtcacacc
    67021 tatattgtaa gctgcacagt tctcaatggc ctgttttttc agggcaagta aatccacccc
    67081 tcgaagcttt ctgtttattt catcgttggt cccgtcaaac tgcataaata caaaagataa
    67141 cccggcctca gccagctttc ttacaaattc tttatcttca gccaaccgta ttccgttgct
    67201 gttaagctgc acatacctgc agcccgcctc cttggcactg cttatgattt cgggaagatc
    67261 atcccttaca gtaggctcac cgccgctgag ctgtaccagc gttttgcccg gtactgccag
    67321 ctgccgcagc caggcctgca cttgcttcaa gcttggatca tctgtattcc tgctgtctgc
    67381 aaaacagtat ttacatctca gattgcagcg gcttgtcacc tccaggagaa cacagcatgt
    67441 cccctgccta tgttcggggc agataccgca agcctttggg caattgaggt tttcgttctt
    67501 ctttattctt ggtgtgtcac ctatccactc accgatagga gtcttggagc gccatattac
    67561 actggaaaag aagccgtgct gtacacactt cttctgtaaa taaacattac tgccaatttc
    67621 aacatgctgc gccgggatgc ggctcagaca aaccgggcat acgctatagg tgcttcgtat
    67681 tattttcggt atactcatct atttgtcctc cagctgcatc tcaacctcag ccatgcgccc
    67741 cttgaccagc tcttcgggaa taaaaacctc accgcaatcc ggacatttaa gtatgtcggt
    67801 gaaaaagctg tggcccaaat acttgaaata tacttttttc ggtatcagtt cctttttgca
    67861 cttacagcat atgataacct tattatccgc catttgtcag gcctcctcta tgctcatccg
    67921 gtgacagtat gcattaatca gattgaattt cccgtcctca ggaaggtatt ctacccaata
    67981 ggtcatattt cctatctgca gatgcccgaa aaaatggccg gattccggaa tctgaacctt
    68041 ccgcccggtg ctttcacaat gctcgatcac cgtctcaata tctgtttcca gtatcatttc
    68101 cttatgcagc ttttgtctga gttccggtgt tataagcagc tttatcctgc tttccttttg
    68161 ttccataata gcttcatccc tccaaaactc gctgagcagc ttgtgcttaa ggtttatgcg
    68221 gttgcttctg cgctgggtaa tggttggcag catttgatcc ttatcatggg atccaaatat
    68281 tatatctaat atatgatata cgggcttttt cgctgcgtca aaaatatcac ggcaattaat
    68341 gcaatatgtg atataaggat tgctgttctg agagattctg gccttaacaa cctcccgggt
    68401 gaaggatggg ttcgcgattg acacctgccc gccccagctg cagcacttgg cgtactttcc
    68461 ctcgtaagga agcggttcaa ggttgaaacc ggccttttcc gctatctctc gtattacatg
    68521 ctgaagctcc ggctcttccc tggtggtgca agggtcaaaa accgatacgg tatctccgtt
    68581 gactcttgga gatggtttaa tgcctaattc agaaattaag ttgtatagaa atatcccctc
    68641 gatttccggc agatatttcc ggaacatttg cctgcaggtg ggacatgcaa agacagcgat
    68701 cggtttcccg agggaaatcc aatcctcccg aagcttggca acggccgcct catgaagctg
    68761 ttcatctccc gcccattcgg caggagcgcc gcagcagccg agtatcaggg ctgtatccgg
    68821 ggttttttcc ctcaggaatc ggtagctttc aataacatac caagggtccg atgctcccag
    68881 ctgacatcct gggaagaata catacctgct tttgtcaaac cccttcggaa gcttacacat
    68941 tcctgctgcc tcgctgttgg tgaattccat atcccgcagc cagaaatcat gaaatgccca
    69001 cggcatggca ccttttcctc gcattgcccg gtggctttgc agcagaaaat cccctgtgtc
    69061 aatttcctgc gggcagattc ctttgcaaag tccgcattgg ttgcaggtag aaataaggcg
    69121 ggttgcgaca gttccgtttc cgtccagtgt tccggggtta actgtaattt ctacttcttc
    69181 tcctattctt ttgggatatt ttccataata gctcatcaag tcacaatgac gtacacaagc
    69241 gtcacaggag caccttagac agcgtttggc ctcatacagg gcattttcct gggtaaatac
    69301 ttctccttca tcgggaagca caggcttcat atagctgata agatcagtat ttatcaatat
    69361 ttccgttttg tcctttgctt taggctgatt catgttaccg gtctttatat accgttcaat
    69421 ggcgtttatc gcctgaagcc catcggcaag ggcctctatt atatttcttc cgcataattc
    69481 tccgcccata aaaacaccgg gtttgtcact ggcaaaggca ccttccggat caggcttcag
    69541 tccaaaatcc gtcccccctt taccggtggc aacatatatg gcatcaaagt ccaactcatc
    69601 cagacttgtt atttccgtat tcaagttaag ggtatacttt tcatgcataa actgccgctc
    69661 aatgtcggcc aaaaaaactt cagggggcag aacatcccat aaatgcccgc ctatcctgtc
    69721 tgttctttca aataccgtta cattgtattt ttttgacgcc aatcggagcg cacatccaag
    69781 tccgctgatg ccggcgccta ttattgctat tttcttattc tttggaggca ggttatagct
    69841 attggggttt aaatccctcg catactttat ggcagccttt tcaagatgct tcatggcgat
    69901 tgcctcgtcc ttcaagcgtc tgggacatac gtttttgcat ggctcatggc ataattcgga
    69961 cacaatgccc gggaatccaa ctgtattcaa ataggttctg taggctgcat taaagccgcc
    70021 gcgctgcatt ttgggcataa agtcatgaat atccagacga aatgggcagg cagcagtgca
    70081 aaaggcaggt tcgttctgca ggcaggtttt tattttttct ttaaactttt ccatttcaag
    70141 taccaatatg cacctttctc agctaggata aatcattatg gtattgttgc gcccgaatta
    70201 tgcaattctg cgtaatccgg gcgcaaatca atatttcagt tcagctgcaa tctttgatta
    70261 gaagaatctc tctgcggtgg cgtcccctaa cgggataaag aaatcatcgc cgccgtactt
    70321 aacgggattt gcctttatgt tttcaagttc atcgtacaag tcggagccaa ggaagtattt
    70381 ctttggcggt tctatctttc cgccggctgc gagtatatcc agccccgctt tcaccttttc
    70441 aggcgtagca ggcatctcat agatgcgcac accacaggca ttatcgatag cgttcagaac
    70501 cgctacatgc ccggatgact ggaaagcttc ggaagctccc gaggaaccga aggggccgac
    70561 ctcatccggc ctttcatagt gaataacatt gatttcatcc ggaatgtcct ttatatacgg
    70621 cacaccggcg cctaacatat ttgaatgctt ttctacatca tcataatttt cactgagggc
    70681 aaagcctatg cagtgagata tgccgccgta tgcctggccg tttacggcat ctatgtttcc
    70741 tattttacct acatcatcca cgcaggtgaa gcgaaggact cttaccttgc ctgtagcagt
    70801 gtcaacttcc acctctgcca ggttcaggca ataagtatag gcaggagtgg gatctccgat
    70861 accggtattg ggatccaggc ggcaaatccc cggaatggtt gtatttgaat atgtagcttg
    70921 atactttgtc ggaattcctt cggctgccat ttcatcatat gtcctgtagg tgccgtcagg
    70981 cttacgcata gcgttcataa gcttttctgc ggcaagtatg gttgctttac cgctcatgaa
    71041 gtgtgtacgg cttgcacctg acataccgct gtccgggcaa agcttggtat cattctgaac
    71101 aagcctgatc tgatccggtt ttacccccag aggcttaagc gcctccaatg ttaacattaa
    71161 ggaaccgata tcaccgccct ggcccaattc ctgataggta tcatatttag tgaaggtatt
    71221 gtcaggatta agctctaatg caacggaaga actgtcggta ccgccttcag tgacattgaa
    71281 gcctccccat gaaagaccta cgccgcggcg cttctgtggt gtatcagctg ctttcgcttc
    71341 cgcaacagcc ttttcataat aaggccgcat aatcttcatt atttcctcca taggatactg
    71401 gcggaacggg tagctgttta tattggtgtc cccttccctt gcaatattta tccagcggaa
    71461 ctcgaaagga tcaattccgg ctttctttgc aagcatatcc atcatagcct cggataaagt
    71521 atacgcctga ggagaaccgt aagcgcggta agctgtaccg aagttatggt tagtgttggc
    71581 tatacgggta aggccggctg cattaggtac cttatagggg aagaaagcaa agcggacttg
    71641 ttttgtcatc agatcgtctc cgccccacga gtaggcacca tgatccatcc caaagtcaaa
    71701 ttcaaccgcc gtaattttcc cattttcatc acaagccgca cgcccgttgg agtggcaggg
    71761 gcagcgtttc cccgaaaaat gctggtgctc ttcatagctc atggataaag ctaccggcat
    71821 ttttgttact acggctgcag cggcagccag gcaaatatct cctgcgttgg tagaccaacc
    71881 gaagctggct cctgtggggt tcatgacaat gcgcaattta tcctttggaa tgccgactga
    71941 atcgcccata cgtccctttg atgaataaac cccttgagat ttgcactgaa ttgtcagatt
    72001 gtcctcttca tcaaagtaag cctggactgt agtaccttca atggtcaaat gcggttcccg
    72061 ggtagaataa aagctaccgg acacactgta agcagaattg tctattactt cctgcacctt
    72121 ggcagcatct tccagaccta cgcccttcaa aaccggctgc atagagaaaa tattgggtgt
    72181 atcctcatga acacgcattg catccggcat aacagcatcc agatagctaa ggtattcagg
    72241 cagcttttca atttcaactt tgacctttgc cgctgcagca cgggcatgct cctttgtatc
    72301 agcaacagca agggctacaa catcgccata acggaatatc ttatcctcac acaacacctt
    72361 acggctggga accataactg tagtacgttc atggaactga gcctcggcca tcacatttgt
    72421 gcctccggcc tccttgagct ccttgtgcgt aataatcttt acaactcccg gcatcttctc
    72481 tgcttcagag gtgtcaatat tcaaaatctt tgcatggtgg gcaaccctgg gctgcgttgc
    72541 tgcaacatgg agagtctctg ccggcatctt aagctcctgg tcatttccat aatcggcaag
    72601 tccacaaacc ttggcaagtg ctgtaggacg aacaaccggc ttaccataat actctttgtc
    72661 ctccggaagt ttaaaggaaa tgtcttctat tgttgcctca ccgcggacaa cctttgctgc
    72721 tgccataaca gcatcaacaa gctgtttata gcccgtacaa cgacaaacat ttcggtgttt
    72781 ctgaaaccaa tccctgacct cttcacgggt tgggttatta ttttcctgca aaagtgcata
    72841 agcggatacg ataaacccgg gtgtacagaa accgcattgc acagcaccat agttcatcca
    72901 tgcgacttgc agcggatgca agttattcgg ggtaccaata ccttcaatag taattacctt
    72961 gctgtactct tcaacggtac tgattttttt cgtacaagag cgaatcactt tgccgttcag
    73021 aataactgaa caagcaccgc atacccctgt accacagcca accttaacac ccgtcaaccc
    73081 catacggcgc agaacagaag ccagcgtgtc cttttccggg ttgcaggcaa acattcgatt
    73141 tgcgccattg acgttaagcc acatttttct aagcttcatt cacatatacc tccttaattt
    73201 ttatgagtct gccaatatta aagataattg taataattat tatctgtctg atggaataat
    73261 attgattgtt tgattatata aaaaacggtg cgcccaaaaa gatcgcaccg catatataat
    73321 caaacaaaaa taaaaatgat tactactgcg attacgactc ctctacaaca ggtggcataa
    73381 gtgtttccct atatacccta atgttcttat tcccatagca taagctttac ttaatagaat
    73441 cggagtcaat tacgtaattc tgttcataat aatgatatac taggcatgtt atttttgtca
    73501 atattggcgt ataatattat tcacaagaga ccctccttgg tgtttatttt gttttgcagc
    73561 taatatttta ccaaaaagag tggtctcttg ctattttttg acctacaaca attttacact
    73621 aaggtattac atgcattcca tagactctga aatgatattc cgaattgctt catctcttac
    73681 gacagccctt gacatttcaa agcgtaaaaa ttatattcta atataaggca catagtgaat
    73741 tcatcttata ttaccggagg tgtttcagag ctatggaaaa catcatgaca ttggaaaaag
    73801 tcaatgagct tcggtcccag ggctttaact gttcccagat tgtgttcctg tacggatctg
    73861 aaaggttagg gctggaaccg gaaaaagcat taaaaatcgc aggagctttc ggcggtggaa
    73921 tgtataatgg tgaaacctgc ggctgtgtaa caggcgcact aatggttttg ggattaaaat
    73981 atggccaatc tgtgccctat gatttagcgg caaagaatct tctcattcaa aaaaagacag
    74041 aatttgaaga aaaatttaaa gaagctcatg aaaccctcat ctgcaaagaa atacttggtt
    74101 atgacatctc caatccttca gaaaaatcaa taattgtgga aaaaggattg ctggacgaaa
    74161 tctgccctta tctcatatgg gacgcttgcc agatacttga tgaaatgctg taaacactaa
    74221 tgctatgtgt ttcaaatcat tgatttccct cccttggaag cacaaatcaa ttccggttat
    74281 ctgcggtaca aaataaaaga taggtacact attgagtacc tatctgcact aaagcttccc
    74341 attacctatc agttacgtta gcgcttaaat ttgaatggat ctttttctcc agataccccc
    74401 tattcttaag catttcccct ataaaggttg agcccaatat caaaatcccg ccaatgatct
    74461 gagtgaatat taacgtctct cccataaaaa tcacactgaa taaaatacca aataaaggtt
    74521 ctaaataact gtatgatatt atttcaacag atttcatatt tgtgtagagg gaaaagaaca
    74581 tggtataagc cacacctgta tgcaataccc ccaacagaat ggtatatata gctgctttgg
    74641 catccaggtg tacagccgtt aaaagatttc cttcaatcaa tacaaatggc agtaaaacaa
    74701 gcatcgctgt aaatatttgc acaaaagtgg cagtttggtt atccactctg actttaatgc
    74761 tgcggttaat aagcacaatg cttgcataaa tcatccctga tacagcacta agcagcatcc
    74821 ccatgtatcc gtacccctcc agtaaattat ggccaacaat cagaaagagg ccaagaaacg
    74881 aggttaaaat cactgcaact tgaatttttg aaatagtctc tttcaatgcc agcggtgcaa
    74941 atatcatgac atataccggg gacatattgt agataataac agcagatgcg atactagtat
    75001 ttttgaaccc atagaacaaa gttaaccagc caaaacccag taatatccct gaaataaggt
    75061 atggctttaa aagcgtccac ttcaacttat ctgctttttt cattttcatt acaacaaaaa
    75121 gaaccggaag cgcaattgat gcccgaagaa atgccaagct taaagcggac aatggaatgg
    75181 attttgtaaa tacacccagg ctgccccaaa taatcataac aagtactaat ttaattctat
    75241 tcataatcat actccttatc tttgataaat ccagattagc agaaggataa ggagctgtat
    75301 tgtataaaat ttacactatc ccattaattg ttttatatat gcccctggcg taacaccata
    75361 cattttttta aattcccgaa ttaaatgggc ctggtcataa aattttaatt ccacacaaat
    75421 ttcaaacaca tcggcgcctt tacttaataa cctttttgct tcagctaccc gatattgtaa
    75481 atggtatgca gcaggtgtgg ttaaatataa cttcttaaaa ttcctgatta ttgaaaattt
    75541 atttagcccg gatatttgct gaagcttatc taatgttata tcatcactga catgatttag
    75601 tatataatca tggatctttt tgatattatc aattgtattt gcagcagcag tttccggaat
    75661 atcatcttct ccaaactcta tcaacaattc aataagtata ttttccaatg tgtttggaat
    75721 cttttccgtt agtagttgct caataaaacc tactaacctc tgaagctgat tacctgttaa
    75781 cttttttgct tgattaaaag ttactaaatc gccatagtaa ccgggatcaa caaataacat
    75841 aacatatgcc cagtgattga tatcattcgg cgcacatctg tgagtcatta acggcggtat
    75901 aattacacca tcacccggtc cgtaatggat agtactgccg ttcaatgtta agtcagtggt
    75961 tccttctacg atatatccca atgagagttc gttatggaca tgaggcttat aagcatgggg
    76021 gtcgttcctg caaagcttga tttccacgcc ttcaataata ctcctgctaa attccatgcg
    76081 gcttttatct gacatattca aatacaaata cctcctccca ttctattaac tactgcacta
    76141 tatcaattca ggtttctgca ctcttaattc tttatataat tataacatca tttccataat
    76201 ttgttcattg ctattgacat attttgcacg aatatattgc agcaataagc agtggtttat
    76261 acaataaaag ataggcattt tttaataatg cctatctcat ataaatatat tagtctgtac
    76321 agtttttcag ataatataca ggttaattac acttcgaata accacagttc ctgcatatca
    76381 cacagccgcc ttcgtgctgt atcgtgctgc cgcattccgg gcaattcttc atattcccat
    76441 tgaagctgcc ataagcattg tatgatatac tgccctcctc aacagtcact gcatggcagg
    76501 gttccgcaca aagggagcaa ttattaatgc agtttcccct ttcctcaatt tccattgcca
    76561 gttcatccat tgtcttgata tccttattat ccaccttggt attcatatat ctttcgaggg
    76621 cccttcctat tgcatcaggg caggaaagta ccttaagccc tttctgtcta agggtcgaat
    76681 gacatctgat gcctctgagc tgctctatta tctcccctat atccactccg gccctcaagg
    76741 caattgaaat aagacggctt gttgcttcag actggcttgg acagcctcct gctcttccaa
    76801 ggtttgtgaa tacctcacaa atgccgtttt catctgagtt taccgttata tataaattgc
    76861 cgcagcctat tttaactttc tcggttactc ccgttgtcac ttccggtctg gttcttggca
    76921 caagaacctc attggccgga gccggttttt ccttattatc cttgtttgcc gagccgatgt
    76981 tcagcacctg ggattcgcgg ctgccgtccc tgtatatcgt cacgcccttg caacccatcc
    77041 tgtaagccag catatatacg gcacttacat catccctggc agcggaattt gaaaagttga
    77101 ctgtcttgga tacggcattg tcggtatact tctggaaggc tgactgtatc cttatatgcc
    77161 actccggctc aatatcgtgg gcagtgacaa atacttttct tatatccttc ggtatgctct
    77221 tgatatcctg aatggtgcca tgagctgcaa ttttcttcat gagttcagtg ctgtaaaagc
    77281 ctctttccct ggcaaccctt tcaaacaacg ggtttacttc gggaagctca tcattatcca
    77341 ttacgtttct tacaaaggac agtgcaaaca gcggctctat accgcttgaa actccggcaa
    77401 ttatggatat ggttcctgta ggtgcaattg ttgttgttgt agcattcctt atcctgatgc
    77461 ctttttcctt aaacacgctg tcctcatagt atttgaaagt gcctctcttt tctgcaagct
    77521 tcattgaagc agcctttgac ctttcattta tgaacttcat tatgctttct gccgtattga
    77581 ccgcttcttc cgagttatat ggtataccca gctctaccag catatcggca aagcccatga
    77641 ctccaaggcc gatttttctc gtcccctttg tcatctcatc aaccattggc agaggatatt
    77701 tgttgacatc tataacatta tcaaggaagt gtaccgattc atctactata ctgcctaact
    77761 tttcatagtc tattaccttc ccgaacttgt tcttcttctt aatgcagctc atgaggttta
    77821 tagaacccaa attgcaggat tcatacggaa gcagaggctg ttcaccgcag ttatgtatat
    77881 atatgccgtt ggcatcaaaa gcgtttatcc ccgggacctg aacatcaaac acttcagctt
    77941 ctcccgattc gataatctcc gtaacggaag caacaaaccg ctctctattc aaagctcgtt
    78001 tgtaattgcc caatccggat tttaagcgat tctgtttctt actgctgctg aagcctatta
    78061 attcatggaa aacatataag ttgtccttag aaataaccag ttcatggcct gcctttacat
    78121 tataatcctt caaaccgcct ttgccgtcag gcattgattt aatgcccggc tccttacgat
    78181 tactgtatat ggcagatatg attcccattc tgcccagcat tctctgcaca gccttaagag
    78241 tatccaggtc attctgccaa agtcttactg acagaccttt ttcctgtgta cccgcaacag
    78301 ttccgtcggt atcgaacata ccccttaaaa acccccgata aaactcatag cttccctttt
    78361 caacctctga agcaatcttt ttgtctccgg gcatgactcc atattccaaa gcaaggtcac
    78421 gaagtgcaga aagcctcatc cggttttctt ccctatcgct gattactgtt tgaaatcctc
    78481 tgaagtcgct tctatgaggc aatgcacagg cagcttcacc tgccgctttc attaaggata
    78541 ctgcctcttc atcgctgccc catacggata ttattcctcc ctctttttta agtgtcccat
    78601 cccctataag cacacctaac agatatcctt catcaaagct tccttttcct tcccatctta
    78661 tactcctatt gttggacata atgattctgt ctccgggcct gagatcccct gccgccttcc
    78721 attcctcatc aatcttaaac cgcgtcatat tcgctgccgt tcttatcaaa tggtcctttg
    78781 tcagcttcag ttcataaccc cggtcggtac aaaccctgat tatacttttt ttgccggtgc
    78841 tgaagaaccc cttttcatct gacatggaat atgaaccgtt tagtgcaagc tgtacctgcc
    78901 tgcctaaaat atccttaacc tgaacagggc cttttcgggt cagaacccat gagtcccctg
    78961 ttatgcacgg attggtgctc tcgatttctc ccagcctggg taccgcatta tccctgttta
    79021 tcctgtcaag gaatatgatt cccggctctc cgttgttcca agccatatct actataaggt
    79081 cataaacctc ttttgcatta agtttcccgg caggtttccc ggtcgcaggg tcaataagct
    79141 cataatcttc gcccttttct gcagcttcca tgaacttttc agtaatggca acacttatat
    79201 tgaaattggt tatatcgctg ttatcctttt tgcactgtat aaactcaatt atatccggat
    79261 gatccaccct aaggataccc atattagcgc ctcttctggt tcctccctgc ttcacagctt
    79321 cggtggcggc attaaacacc ttcataaagc ttacgggccc tgatgctacg ccgccggtag
    79381 tcgccacagc cgatcccttt tttcttaagc ggctgaagct gaagcctgtg ccaccgccgc
    79441 ttttatgtat cattgcagca tttttgatag catcaaaaat accctccata gaatcctcaa
    79501 caggaagcac aaagcatgct gataactgtc caagctccct tcccgcattc atcaaagtgg
    79561 gagaatttgg aataaactca aggctgctca taagcttata aaatttttct tctgtttctg
    79621 caacatctgc cggactgccg taaaaactat cagcagccgc tattgtgctt gcaactctcc
    79681 tgaacatatg ctctggggtc tcaaccggct ttccgttctc atcctttata agataacgtt
    79741 tttccaatac tttaacggca ttgtctgtta acacggtaaa tcaccacctc cggtctgtat
    79801 tttgtatctt gctgcgtatc tgaacactat atattgtatc atatggcaaa atataatact
    79861 atttatattt gctaaattat ttgtcaaaat ttcgacaaat aacggggaca tggggacgtt
    79921 cggggacatg gggacgtttc tcctgtcccg ctgaaagcgg gacaggagaa acgtccccat
    79981 gtccccgaac gtccccatgt ccccaaaata tcttcttgta cctgttcctg ctctattcct
    80041 tgagcaatgc gcctgaagtg aatacattgc ctgcgttcca cagcagccat tcttcctggc
    80101 ctgcatcata agtcgcatct atctgagccc ttacttcggc agggccgtat cttttatacc
    80161 tatcaagata tgaagctgta aaatcctgaa gccacggcct taataccgcc ttcctcctta
    80221 catccttcat ggcatttatt ctttcctgtg caacggacat acttcggtta actattttat
    80281 agggttcaaa gtccggctcc gccaccccat agctcccttt tccgtaatgg gacgggtaaa
    80341 ccatcggaca tattacgtca acacttttgg ctattgactc caggtgctgg cctattcgca
    80401 tatcgtccgt ggcagttgta accagcccga aaacatcagc tgataccaca actcccattt
    80461 tgctcagcct ttcccttccg taggacagaa attccgcaat tgcctctgct ttggactttc
    80521 ctgctgcact ggtgccgtag tcgataatct ttgtgttgcc gtcggtagga aatcttacat
    80581 aatcaaattg tatctcatta aaaccttttg ctgcagcttc ttctgcaatg tcaaccaaat
    80641 aatcccatga ttctctgttg tatggattca gccaggacat tccgtgccgg tccctccata
    80701 tcccgccgct ggtattcttt accgcaagat tggcatattt gtctccggct atcctatcct
    80761 tgaaagtaac tattctggct atggaatata tattattgcc acgcagtatc ttcattactg
    80821 catctatatc ctttatgcgt acttttttgt ttgctcccgt aaaatccaca tcaggaaggt
    80881 cgctggagta agtcatcaat ccgttgtcat ccttcacgtc aattaccacg gcatttaatt
    80941 ctgtagtctt taccaggtca atcagcttgt tgaacctttc ggtcatggca acagtatatc
    81001 cggtcatata gatccctttt actttgagcc ttttcttcat cagctcccag tcgggaccct
    81061 cctgctgcaa gtagcaatct ttttcataag ataaatcaat ttcgtttttt ccataaatat
    81121 atctctctgc atttgctgcg ttcatttgaa acataattcc caaaagtatc actgctgctt
    81181 gtattgtcca ttttctcatc atataactcc tcgataaatg aagtaatttc tgataatagt
    81241 atatcataaa agcaatacgg atattatcct tatcgctgtt tatgccaaaa aatgcgcctt
    81301 atggcgcatt ttgtcaaata atatcttata atgctgtctc cgctatataa tgacagccgg
    81361 tatgaaaagc ggcggcgtta tcatgcctaa tcttcgaata ctgcgccggt attggcagaa
    81421 gtcaccatcc tgcggtagcg cttgagatat ccgttcggta caggtttttc aaaaggtaca
    81481 aattttgtct ttctctctgc tatggttttt tcatcaacca aaagttccag tttattattc
    81541 ggtatgtcta tactgataat gtccccgtct tcgacaatac caatcagtcc gcctgcagca
    81601 gcttcaggag atatatgccc gatggatgcg cctctggatg caccggagaa tctgccgtcg
    81661 gtaatcagcg ctacactgct gtccagtccc attcctgcaa tagatgaagt cggtgaaagc
    81721 atttctctca taccgggacc acctttaggc ccttcataac ggacgacaac cacatctccc
    81781 ggtacaactt tgccgcccat aattgcagcc atgcaatcct cttcgctgtt gtataccttt
    81841 gccgggcccc tgtgaaccat catttcagga agcactgccc ccttcttgac tacggctccg
    81901 tcaggtgcca aattgcccca aagtattgca agtcctccat cctgggagta tgctgtttct
    81961 tttgtcctta ttatctccct gtcctttacg gttacatctt taaggttttc agctgctgtg
    82021 caacctgtta cggttatgca gtccgttttc aacaacccca ttccttccag ctctttcaaa
    82081 actgcctgta caccgcctgc ctgccacaaa tccgacattg cattgggtcc ggcaggggca
    82141 agcttaacaa ggtgcggtgt cgctttgctt atatcattta tatctttcaa atcaattttc
    82201 aatcctgctt cgtgtgcaat tgcaggaaga tgcagcacgg tattggatga gcatcccagt
    82261 gccatatctg ctgtaagtgc attgtgaagc gaatcagctg taactatatc tcggggcctt
    82321 atattcttct ccagcatttc cataaccttc atgcctgcca gtttggcaag acggacacga
    82381 gccgcattaa cagccggtat tgttccgttc ccgggtaagc caagcccgat tgcttctgtc
    82441 atacagttca ttgaattcgc agtgaacatg cctgcacaag agccgcagcc cggacaagaa
    82501 taattttcat aatccagaat ttcctgttcg ttcattttgt tagcccctgc tgcgcctaca
    82561 gcttcaaatg tgcttgaaag tgtcagtttc tttccgtcca aaaaagccgg catcattggt
    82621 ccgccgctta caaatacgct ggggatattc agccttaaag ccgccataat cattcccgga
    82681 actattttgt cgcagttggg aatgaacacc aatgcatcaa agggatgcgc cattgccatg
    82741 atttcgacag aatcggcaat atgttcacgg cttataagag aatacttcat tccttcatgt
    82801 cccattgcca atccatcaca tacgccaatt gcaggaaatt ggaagggagt ccctcccgcc
    82861 atccgcactc cggcttttac tgcttccata atatcgtcca gatgcagatg accgggaatt
    82921 acatcatttt gtgaattaac gataccaatc ataggccttc tgatctcttc gtcggtgagt
    82981 cccagtccct ttaacagggc cctgtgcgga atccgctcta tgcccgcctt catttgatca
    83041 cttctcattc agttttccct ccgtttcaga ttcattaata taataggatt aaagctgaag
    83101 caagcacctc attggaaatt aaatgttgta tgacgtctcg aaaacttttc tgatgaataa
    83161 ctgataatca aacacaatat ccataagagt tgtatgatgt cttaatccaa tactatgcct
    83221 tttttgtcat aatgtcaaca taattataaa attttatata gagagatact acctctttct
    83281 taaataaaaa actataatgg gctttggaaa aaatgtggta taatgtctta tataagttta
    83341 aaggagaaat aatatggcta atgcttttat acctatatca caaaatattg caaatgaaat
    83401 tagcgacatg atctttttac agaaaaaata taagcctaat gataagctgc caaatgagca
    83461 tcaattggct aaagagcttg gtgtcagccg tacaacaata cgtgaggcgg taaaaatact
    83521 tgtagcaaat ggtgtgctga caatagagcg aggccgcggc acgtttgtta cggaaaggcc
    83581 ggattcgcaa aatgacccct ttggaatctc atatatagaa gacaagaaga aattggtgca
    83641 taactggttt gagatgcgcc tgataattga acctgctaat gtgcggatgg tagtggagcg
    83701 ggcaagtgat gaagaaatac aggaaataat agcatatgaa aaagaagccg ccgaactgat
    83761 agcatccggc aagtcatttt cagcagccga ccagcagttt catgcagcca ttgcaaaggc
    83821 aacgcacaac agcgtaattg aattgatgct gccggcaatt gagttggcta tcagagacgc
    83881 tatcagtaca gcagaatatg taggagttca tgaacgggct attgaaaatg ctctgatcaa
    83941 tcatcgcaat atcgctcact ttattgaaca gagagatgcc gatggtgcgg ctcttgccat
    84001 gtattatcat attaaaagag gaatgactga tttggatttt taaaaatgat atatttcatt
    84061 aaatttaaaa agatatgatg aatgtctttt ctgatattca ccatatcttt ttaatgcaaa
    84121 aaataaaaat ggctccccaa gttggactcg aaccaacgac cctgcggtta acagccgcat
    84181 gctctaccgg ctgagctatt gaggagtaat taaatctggc ggcgatctac tctcccggga
    84241 ccctgcggtc cgagtaccat cgacaccgga
//
LOCUS       DAOOAB010000004        78624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_357_length_78624_cov_16.838550, whole genome shotgun sequence.
ACCESSION   DAOOAB010000004 DAOOAB010000000
VERSION     DAOOAB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 78624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 78624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_357_length_78624_cov_16.838550"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKU88_01400"
     CDS             complement(<1..637)
                     /locus_tag="PKU88_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPX67979.1"
                     /translation="MKIAVLSGKGGTGKTLISVNLAAAAGKSVYLDCDVEEPNGHLFF
                     KPENFYEEEVSIKIPVADEKQCSGCRVCVDFCKFNALAYIKDRLVVFDEVCHSCGGCI
                     LLCPEKALNEKEKIIGKIQKGVSGQVTVNTGILNTGEASGIPLIKKLLDENRKVKGMT
                     TFIDCPPGSACIVMESIRDADYCILAAEPTLFGVHNLNMVYELVKLFRKPYG"
     gene            complement(639..1007)
                     /locus_tag="PKU88_01405"
     CDS             complement(639..1007)
                     /locus_tag="PKU88_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPX67980.1"
                     /translation="MKIAIPVDDKNMEASVCISFGRTPYFLIHDTESNRSLFFENSAA
                     ASQGGAGIKAAQFLVDHKADILLTPRCGENAAEVIKAANIKMYKTIPGSAKDNVNAFT
                     EGKLSLLEEIHAGFHGHGEK"
     gene            complement(1022..1375)
                     /locus_tag="PKU88_01410"
     CDS             complement(1022..1375)
                     /locus_tag="PKU88_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPX67981.1"
                     /translation="MPRPRKWRKVCCLPESNRFGPLGSPADESNYIVMTVDEYETIRL
                     IDLEGFTQEECASRMNIARTTVQGIYVDARKKLAESLVNGKVLWIEGGEYRLCDGSGN
                     ACGRGGCRRQRFGSK"
     gene            complement(1438..1686)
                     /locus_tag="PKU88_01415"
     CDS             complement(1438..1686)
                     /locus_tag="PKU88_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPX67982.1"
                     /translation="MPGRDGTGPMGAGAATGRGLGFCSGVNAAGLGFGCRRGFVRGYG
                     KGFAVNWASAKTQKEMLQTQKDLLESRLKIVNEQLENI"
     gene            complement(1775..3328)
                     /locus_tag="PKU88_01420"
     CDS             complement(1775..3328)
                     /locus_tag="PKU88_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPX67983.1"
                     /translation="MSYSPSLGSSLTNTKMRTPEHISDFSGMCSVCTASCIGTCEIGL
                     SAVRGSEAIYPFETDKNQFASEKSYPLDFSHFNINGRVFGALGCPEDANEATFPKANI
                     AVDFGINNKIKLKAPIILPAMAKLNWKDYYAGAALSGVLVVIGEDAVAKDKELVLENG
                     KVAGSPLLKEMVSSFRQYYRGFGDIILQGNYDDENLGVLDYAIKELGVKTVELKFGQA
                     AKGIQGMGRVKDIDAALEYQKKGYLVYPDPSDPEIAKNYKEGKGQVFEKIGKLPMWNE
                     DILINRVKELRKLGAERICFKTGPFDPKDLIRILKIASKAGVDLVTFDGAGGGSGNSP
                     VKMMNEWGIPTVYLESLLYNILKRMKEKNYNLPQVAITGGFAMEDQVYKGLALGAPYI
                     NIVGIGRAAMAAAMAGKQIGDLMKEGMTPKEYLRFGTAKEEVFGDIRELKLLYGEDAL
                     NISTGAVGLYSYINRIAAGLKQLMALNRKFSLEYIDRSDIIPLTEIASKVTGLEAVYR
                     YNNELDKEI"
     gene            complement(3527..5059)
                     /gene="guaA"
                     /locus_tag="PKU88_01425"
     CDS             complement(3527..5059)
                     /gene="guaA"
                     /locus_tag="PKU88_01425"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPX67984.1"
                     /translation="MADKELVLILDFGGQYNQLIARRVREANVYCEVVPYDYSLDEIR
                     KKNPKGIIFTGGPASVYEEEAPKCDPGMFEMGIPILGICYGAQLMAHMLGGKVARAVK
                     REYGKTQVKLLENTLFREVPQDSICWMSHTVYVDSPPAGFDIIGATETCPVAAMGSKS
                     GKLYGVQFHPEVEHTEYGKQILRNFLYDICGLSGDWTMGNFIDENVERIKELAGDKKV
                     ICALSGGVDSSVAAVLVHKAIGNRLTCIFVDHGLLRKNEAEQVEEVFRKRFDINLIKI
                     NAQDRFLDKLKGVSEPERKRKIIGEEFIRVFEEEANKLGEIDFLVQGTIYPDVIESGT
                     KTAAVIKSHHNVGGLPENMRLKLIEPLRELFKDEVRLVGLELGLPEDLVWRQPFPGPG
                     LGIRVLGEITKEKLHIVKESDAILREEIKNAGLERCIWQYFTALPDIRSVGVMGDERT
                     YSHTVAIRAVTSSDAMTADWARIPYEVLEKISNRIVNEVEHVNRVMYDITSKPPATIE
                     FE"
     gene            complement(5150..6601)
                     /gene="guaB"
                     /locus_tag="PKU88_01430"
     CDS             complement(5150..6601)
                     /gene="guaB"
                     /locus_tag="PKU88_01430"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPX67985.1"
                     /translation="MEKFVKEGLTFDDVLLVPAKSEVLPKNTDTSTMLTRKIKLNIPL
                     MSAGMDTVTESKLAIAIAREGGIGIIHKNMSIEKQAMEVDKVKRSEHGVIVDPFHLSP
                     DHVISDAMELMERYRISGVPITDEKGKLVGILTNRDLRFETEFSKKISEVMTKENLIT
                     APVGTNLEQAEQILKKHKIEKLPLVDDKGYLKGLITIKDIEKAIQYPNSAKDPGGRLL
                     VGAAVGVTEDMIERAEALVSSKVDVVVIDTAHGHSKGVIEAVRRIKGRFPELQLIAGN
                     VATAGAVKELIEAGADAVKVGIGPGSICTTRVVTGVGVPQITAIYDCAAEADKYGISI
                     IADGGVKYSGDIVKAIAAGASAVMLGSLFAGTAESPGEMEIYQGRSFKVYRGMGSLGA
                     MACGSKDRYFQEDARKLVPEGVEGRVPYKGPLAETIYQLVGGLKSGMGYCGTHTIEQL
                     RKNTQFMRITAAGLRESHPHDVSITKEAPNYSL"
     regulatory      complement(6659..6760)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            6991..7173
                     /locus_tag="PKU88_01435"
     CDS             6991..7173
                     /locus_tag="PKU88_01435"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HPX67986.1"
                     /translation="MLLFYLNTYNKNSISSLTLIPSSGGVLKVKLNNELIFSKKSAGR
                     FPKEDEVEKLPIARLQ"
     gene            complement(7186..7716)
                     /locus_tag="PKU88_01440"
     CDS             complement(7186..7716)
                     /locus_tag="PKU88_01440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67987.1"
                     /translation="MNDYQIGRPKPEEICSINEFFEIVVRDTFAKNGISHLTELMEGE
                     IECKKKYLMQDMDSEGRDRFFLVVRDKGRIIGTIEYGRPNELIMKCTKGRYIDVIEIG
                     TVFVHPDYQYKGIGSMMLRMMYDELKNKGYTEFCLDSGYKTAQKVWTGKWGKPSCIME
                     DYWGKGAHHLVWRVDL"
     gene            complement(7709..8275)
                     /locus_tag="PKU88_01445"
     CDS             complement(7709..8275)
                     /locus_tag="PKU88_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX67988.1"
                     /translation="MKTNTMFSDVEIKLKKRNKILFSRDSKCLQELIKLIQLQNHRTL
                     VMWALDCAKLPLEQFEAKYPDERRPRICLELCEAWARGKIKMPTAKKAILNSHAAAKE
                     INDSEYGALCHAIGHAGATVHVGTHALGLPMYELTSIVLRYGKDNFLIPVSEKINYYY
                     KRLLYWQENTDKLKLDWADFLLRGDFNE"
     gene            complement(8276..8770)
                     /locus_tag="PKU88_01450"
     CDS             complement(8276..8770)
                     /locus_tag="PKU88_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67989.1"
                     /translation="MVEIIKASEEYAESFYQALDVVARERKYLGIVKGFPLQGVKDFV
                     SMNVANDYAQYYAIDGNKVIGWCDIIPKRNEGFTHVGILGTGLLSEYRNQGIGSKLIE
                     KAMEHAVKKNGIEKVELEVFESNINAIRLYEKFGFVYEGKRIKSRKLDGVYDNVVMMG
                     KEVL"
     gene            complement(8858..9709)
                     /locus_tag="PKU88_01455"
     CDS             complement(8858..9709)
                     /locus_tag="PKU88_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HPX67990.1"
                     /translation="MTKGEKEKMSSGDYYSAVDEELVRERDYARNLTYEFNHTRPTEK
                     ERRLEIIVLSLLPGILILAFYLLVSWYLVPFSIPSILVIFVAIAVILVPFELGFLIYQ
                     GRKVNKRISLNNVVLFREKVPVIQFIILVVILIKWSEFVFRCLSTRIDTLFVNNFFYW
                     VPKWFFLDSFYQHISQYSKTVLLVTWILGLIFNGIIGPIVEELYFRGYLLPRMKHMKG
                     WAPIINVLLFSLYHFFTPWQNVTRIIALLPLVYVVWWKRNIYIGIVTHCALNITGMIG
                     MLPIVLK"
     gene            complement(9959..10801)
                     /locus_tag="PKU88_01460"
     CDS             complement(9959..10801)
                     /locus_tag="PKU88_01460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX67991.1"
                     /translation="MFNNLKAINERPKPFQYYTAAELWTDEHTSKKMLDYHLNEEIDV
                     SSRNARFINRSVSWIIEKFNLNNKSNVLDFGCGPGLYTNRFAERGINVTGVDFSKRSI
                     EYAAQIASSKNLKVNYVHKNYLEYETQDRFDLITMIMCDYCALSPEQRGIMLNKFKEL
                     LKHSGNVLLDVYSLKSYNRREEIAAYEFNHLNNFWSPEDYYCFVNTFKYCDEKIILDK
                     YTIIEKAQTRVIYNWLQYYSVETLRREFEDNGLEVLEFYSDVCGLPYDAESLEFAVAA
                     RQKN"
     gene            complement(10863..11213)
                     /locus_tag="PKU88_01465"
     CDS             complement(10863..11213)
                     /locus_tag="PKU88_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HPX67992.1"
                     /translation="MSASVKPNITIDILDKIDIRVGTIISVEDIKDSNKLVKLSVDFG
                     DFLRTILVGMKGERENPKEIEGQQALFVVNLAPKKMAGELSEGMLFDIGYADGIVPVL
                     AQPEKAVPNGTRAG"
     gene            complement(11238..11369)
                     /locus_tag="PKU88_01470"
                     /pseudo
     CDS             complement(11238..11369)
                     /locus_tag="PKU88_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
     gene            complement(11471..11872)
                     /locus_tag="PKU88_01475"
     CDS             complement(11471..11872)
                     /locus_tag="PKU88_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPX67993.1"
                     /translation="MRSELIAPCGMNCRLCMAFQRKKNKCIGCNSDDCTKPAYCLHCI
                     IKNCVTIQNSSSGLCYECDKFPCRRLKQLDKRYRTKYHMSMIENLENIKQNGMKEFLK
                     NEEARWACIECGNIVSVHRNACPICKTQYIE"
     gene            complement(11969..12202)
                     /locus_tag="PKU88_01480"
                     /pseudo
     CDS             complement(11969..12202)
                     /locus_tag="PKU88_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(12218..12601)
                     /locus_tag="PKU88_01485"
     CDS             complement(12218..12601)
                     /locus_tag="PKU88_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPX67994.1"
                     /translation="MKAQINLITIWADDINKMKNFYNQVLGFKISNDLGNYVEFENEG
                     VRFAICLREVMYAYSSEYEKKTGGQAFELAFPCEDPEDVDKSFEWLISKGATPIHQPQ
                     NMPWNQRTALFADLEGNIHEIFAEL"
     gene            complement(12661..13413)
                     /locus_tag="PKU88_01490"
     CDS             complement(12661..13413)
                     /locus_tag="PKU88_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002066342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPX67995.1"
                     /translation="MRLVEIERFIISLFESSENEHFHEQSGTTVEGTEEIRRIGYCTN
                     LTLDTIEEGKKNNVDLIITHHDAWDFIFGLKDACREKLSEYNISHYFTHLPLDDCNFG
                     TNNSLIEKLGLSIIKKSHNEDGFYCGRIAEFDEEVEFEELVRKLEMILGEPTKAWKFK
                     DGCIKRVGLVCGGGGSTADVREAFEKNCDVYITGEKVLYTIEYAKFVGMNLIIGSHTF
                     TELFGIESLARKIADKYRGVNIIRLSEEHLEA"
     gene            complement(13577..14029)
                     /locus_tag="PKU88_01495"
     CDS             complement(13577..14029)
                     /locus_tag="PKU88_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67996.1"
                     /translation="MEVISIKKSPEYKDRAIKYLQSKWASPNSMKVYDDCITNCITTL
                     SPLPQWYLLIDNDRIIGCAGLITNDFISRMDLYPWVCAVFIEEEYRGNAYGSLLIEQA
                     KIDAKAGGFSHLYLCTDHIGYYEKYGFKYIGTGYHPWGDSSRIYDAAL"
     gene            complement(14148..14591)
                     /locus_tag="PKU88_01500"
     CDS             complement(14148..14591)
                     /locus_tag="PKU88_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67997.1"
                     /translation="MEVKPIKGNKKLFLDLLLLADEQEDMIDKYLERGEVFALYDGDL
                     KSICVVTQEDENTCELKNLATYEKYRGKGYGKYLISYIFDYCRDKCKTLLVGTGDIPW
                     IISFYEHCGFEVSHKVENFFTNNYDHPIYEDGIQLVDMVYLKKDF"
     gene            complement(14626..15354)
                     /locus_tag="PKU88_01505"
     CDS             complement(14626..15354)
                     /locus_tag="PKU88_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPX67998.1"
                     /translation="MYTFTNQFMSNNKNVEFLKANMMGPNAMRLAEELASHLNINENM
                     RILDLGCGCGLSTLLLVKKYGVSVFAADLWISPTENYERFESVGIDEKAVPIFSDATK
                     GLPFANGYFDLLFTVDAYHYFGDTAEMLPSLIPFVKKEGHIAVAIPGLKYEFGENVPD
                     EMQPFWDSDMERTLHSLDWWKDLWKSAKGIEVMDIREMGCCRQAWEEWLASDHPIAAE
                     DIKMMEAEGGKYFNIIQLIAKVKR"
     gene            complement(15911..16372)
                     /locus_tag="PKU88_01510"
     CDS             complement(15911..16372)
                     /locus_tag="PKU88_01510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX67999.1"
                     /translation="MTVRIAKLSDIDVIYDLICDMENSKLDYERFQEVFKKYLEDDRY
                     FCLVAEHSGTVIGCLNLRIEYQLHHTAKIAEILELAVMDKYRSKGVGKKLFEKASEIA
                     KDNECLQIEVCCNQLRSRAHNFYEKQGMHNFHYKFSLNFKEEEIKENRLGL"
     gene            complement(16497..16790)
                     /locus_tag="PKU88_01515"
     CDS             complement(16497..16790)
                     /locus_tag="PKU88_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68000.1"
                     /translation="MGITPFKSSGRGPAILRNCILGVWVLMFAFKQSTTFMARNSGEV
                     TEIVAGLLIIFFSLYDTYVRREKNDVWGIPTWILGGLILTIGILFYLYKNPFA"
     gene            complement(17207..17752)
                     /locus_tag="PKU88_01520"
     CDS             complement(17207..17752)
                     /locus_tag="PKU88_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68001.1"
                     /translation="MINEREKVIKAKEVLIKIANGINPVDGTPINEESFLNDPRIIRC
                     FFYISELLNRVIAGQTNQNLPKPTELHITKEEKSLVKFPENKIGVNEFAKCINNVIDL
                     NKSKKLSGVELNKQLKKMGVLSENIDEQGKVRTIINNKSVDYGFESEKRNYNGNEYEM
                     VVINDIGKAYILENLEDIMKY"
     gene            complement(18000..18191)
                     /locus_tag="PKU88_01525"
     CDS             complement(18000..18191)
                     /locus_tag="PKU88_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX68002.1"
                     /translation="MKNKIKQLRKNKGLRQEDIAMLLNVSRQTIVAIENDKYNPTLEL
                     AMKMARLLDTTVEELFILE"
     gene            complement(18188..18553)
                     /locus_tag="PKU88_01530"
     CDS             complement(18188..18553)
                     /locus_tag="PKU88_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68003.1"
                     /translation="MKSLFSKLGFRKMDEMEKNIALKAQQNALIYVLLALAMWSFYES
                     FKAYSQHTSINSIPSFLLVTTSLVQVFSQLVLQKRVVKDDDEYKDDNPLLKIIIVGVI
                     ISAIIVSIGALLIVSGIQK"
     gene            complement(18949..19740)
                     /locus_tag="PKU88_01535"
     CDS             complement(18949..19740)
                     /locus_tag="PKU88_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPX68004.1"
                     /translation="MKIISIISSSRKNGNTENLVSILEEELKSVSELEHEEIEIERIY
                     LGQANLKMCMGCRICFDKGEQFCPLKDDLLTIREKVVEADGVILASPVYVEDVNGIMK
                     NWIDRMAFYCHRPALAGKVAVTITTSGAGSTNHALKTISFAIMSWGAYLGGQYKFRTG
                     ALMKREEIYEHNHSKVNEVARKLIDDIKNQKAFMPTLYSLIIFKVQQKLWQKNTDSQN
                     SIDYTYWNNKGWLEPKCVFYTTIRTNWFKVKIARLFGAIIALFFK"
     gene            complement(20165..20524)
                     /locus_tag="PKU88_01540"
     CDS             complement(20165..20524)
                     /locus_tag="PKU88_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68005.1"
                     /translation="MSQNNKSLNKIKWAARISAMVIIAFGLPFYFGYGNPLPFINPDY
                     TLWDNMWLMIFPIMFIGLALGLKFEKTGGYLVIISVGLGLLMGLFMQGELVSHMLVPL
                     LVGVLYLIVGYKSDKNY"
     gene            20978..21253
                     /locus_tag="PKU88_01545"
     CDS             20978..21253
                     /locus_tag="PKU88_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPX68006.1"
                     /translation="MPIIRPISDLRNNFNDISEICHTESEPVFITKNGQGNLVVMSMA
                     LYEKQQALIELYQKLSVAEAESKYSDNRISHKNLMKELRETINGKEV"
     gene            21237..21542
                     /locus_tag="PKU88_01550"
     CDS             21237..21542
                     /locus_tag="PKU88_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPX68007.1"
                     /translation="MEKKYKIEYLPSSAKDLAEIVDYIKIDSPQSALNFLDKIDESIS
                     RLEQFPFIGVIPKDIRLQSLGYRILIIDNHLIFYVVFDDIVEIRRIISGKRKYDFLF"
     gene            22035..23243
                     /locus_tag="PKU88_01555"
     CDS             22035..23243
                     /locus_tag="PKU88_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HPX68008.1"
                     /translation="MNNPALCIDVSKSSSFATGFLSYNKPYSKPFDFSHTPDGMKSAL
                     HCLKELQDNSNSKPDVVLEATGNFYKPVTQYFIDLGYSVYVLNPLQTSMEKRKSIRKI
                     KTDPVDTYRIAQVYYTNDLKPFKEQSHDSQELRSLCRQWDGFNTLNTETQLRFRSILA
                     LVFPKYDSVFDHLCNPTSLRVIAYFPSPKAILSAKREELFNLIKLCRHSNEWCNHKVD
                     MLIAAARESLSFDGAQQSNIRVLQSYVKSLLTHKEILADIRAQINEWTKLSHNFQLLL
                     SIPGVGEVTAATILGEIGDISNFDTAKQLVAYAGLDPSVYQSGSFRAKNTKISKRGTP
                     YLRKALYQAASAAVRKRPKGPCNSVLYEYYTNKVNAGKPPKVALVATCNKLLRIIFGI
                     LSSKTEFKAE"
     gene            complement(23945..24331)
                     /locus_tag="PKU88_01560"
     CDS             complement(23945..24331)
                     /locus_tag="PKU88_01560"
                     /inference="COORDINATES: protein motif:HMM:NF022172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HPX68009.1"
                     /translation="MKLNHDCIRALLIYLEENLEVKANGLPEGLKLGHIDEDDLSGFS
                     QEDIFYSAKKLVEAKYIEVANKEVAPRAMIIKEITWNGHDYLDSIRDPKVWYEIKSRT
                     KDLSGIALEVVKSLAIEITKQMLGLG"
     gene            complement(24487..25542)
                     /locus_tag="PKU88_01565"
     CDS             complement(24487..25542)
                     /locus_tag="PKU88_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPX68010.1"
                     /translation="MAICVRKGKPGVLELVFPYNEEYIAKVRGIPGRRWDKDNKKWLV
                     PDCEESINQLQELFTKEELVIVKGSSNDQFLKKMEEGLKLRGYSQKTIKSYMSHARLF
                     LGYCGKNANQLVKEDINCYLLYLLEQKGNAHSFVNQAVSSIKFLFGKVLMEDDLTLDI
                     VRPKKEKKLPEVLSQNEVRHILDNVTNFKHKAILFLIYSSGLRVGEVVRLKLKDIDSD
                     RMLIHVVQGKGRKDRYTVIAEVALGVLREYVQKFRPVEWLFQGEEVGRHITERTVQRV
                     FENAKEKSGIQKKVSVHSLRHSFATHLLEGGIDLRYIQELLGHQSSKTTEIYTHVTEK
                     GIKNIASPLDKLMSRRE"
     gene            complement(25826..26137)
                     /locus_tag="PKU88_01570"
     CDS             complement(25826..26137)
                     /locus_tag="PKU88_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPX68011.1"
                     /translation="MPDFGNSFSGLAKDRKLTDQELIRAIRFMVAAEYEAIQLYMQLA
                     ESTDNMLAIEVLKDIADEERVHAGEFLRLLYELAPDEEKFYAEGAEEVEEEIEAFKRK
                     K"
     gene            complement(26236..26679)
                     /locus_tag="PKU88_01575"
     CDS             complement(26236..26679)
                     /locus_tag="PKU88_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPX68012.1"
                     /translation="MKGKVLAVNISEKKGVFKKPIEQGEFKVDHGLAGDAHAGNWHRQ
                     VSLLGIESINKMKALGIEGLSTGKFAENLTTAGLELWKLPVGTKLKIGETEQEVTQIG
                     KECHVKCEIFQKIGSCIMPTEGIFTKVLKGGIIKPGDEIEVQGDV"
     gene            complement(26697..27677)
                     /gene="moaA"
                     /locus_tag="PKU88_01580"
     CDS             complement(26697..27677)
                     /gene="moaA"
                     /locus_tag="PKU88_01580"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPX68013.1"
                     /translation="MKDELGRNINYLRISVTDRCNLRCRYCMPEEGIEKKEHLDILTL
                     EEIFEVVRVCADLGTNKIRITGGEPLVRNGLTGLIERITALESIKDLALTTNGSLLKK
                     YARELKKAGLKRINISLDTMDDKKYEYLTRGGKLKDVLEGFHEALEVGLKPIKINTVL
                     TKGFNEDEIGDFIRLTQNYDIDVRFIELMPLGHAAGFASKHYISNLVVLERYKELELA
                     EAADKSSPAMYYKLPGAKGRVGFINPISHKFCDNCNRLRLTADGKLKPCLHSNLEIDV
                     KGILRGRNSDDRYLALQKAVEEAVQAKPGHHTLNDLYNKPIDRDMYTIGG"
     regulatory      complement(27790..27921)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(27962..28774)
                     /locus_tag="PKU88_01585"
     CDS             complement(27962..28774)
                     /locus_tag="PKU88_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPX68014.1"
                     /translation="MEKRMLTPKEIAMATCDAGAAKAKLSIAQMMVLGIFAGVFIGFG
                     AQGAITIGQTLAKVDTGLQKFAFAGAFPVGLMLVVICGAELFTGNNLMTLGCLNCRYK
                     VSDILRNWFFVYTANFIGSVILAAIIANTGLMAAGTPIAESAIKIASNKVAIPFGAAV
                     LRGILCNIMVVLAVWMATGAKDVISKIFACWFPIMLFVLCGYEHSVANMYFIPLGMFL
                     GAPVTWGQIWINNLIPVTIGNLIGGALIVPLFYHVAYMKAPTAAAPTKDAKA"
     gene            complement(28920..29453)
                     /locus_tag="PKU88_01590"
     CDS             complement(28920..29453)
                     /locus_tag="PKU88_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPX68015.1"
                     /translation="MLEKILEHNRKFVQERKAAGEDKPISGHAHKDIMVFTCMDTRLV
                     ELLEPAMGFGRGDIKILKNAGNIIRNNCSDVIRSVAVGTVLMGLKEVYVVGHSDCGMR
                     KQKPEDIRAKMRERGIPEEAIEAIDIKEWFGLLQDEAENVKDAVLKLKNSPYIPKDVK
                     IHGLLMDPNSGEISVIE"
     gene            complement(29719..30204)
                     /locus_tag="PKU88_01595"
     CDS             complement(29719..30204)
                     /locus_tag="PKU88_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPX68016.1"
                     /translation="MKIGVISDTHIRSSAKLLPNIIYSVFDGVDAIFHAGDILIDEVI
                     IELETIAPVYAVAGNNDSYEMLYKYSTKRIIEANGKRIGLTHGTSRVNAYAGAYSMFA
                     KNNVDCVVYGHSHKPHNEVIDGVLFFNPGSPTSKRFESRYSLGLLHIGRDIRGEIIYF
                     D"
     gene            complement(30182..31000)
                     /locus_tag="PKU88_01600"
     CDS             complement(30182..31000)
                     /locus_tag="PKU88_01600"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HPX68017.1"
                     /translation="MNFFVQMRESVIDFKFYRSIRDNKFSRSFVYLLLLFLIIYFING
                     TRTFIVTRIFMDDLAVHLAENVPEFRLENGVFSFEGDMPYYISSSTNEAFIIDTTGQV
                     TESILEDAGTGMLITRDKIFVKRNKIETRQFSLSELEGFTLAKSDVLEFMPKLSWIVF
                     ILIAFSFIFVLGWKLLNAVILALLGIAVNALLKGRLRYNNLLNISIYALTLPMLVQLA
                     VNLYGYPIPGFGLIYWLISILYLDLAIKSCIDQDKESEAGNELEDIKGEDRSNF"
     gene            complement(31098..32102)
                     /gene="amrS"
                     /locus_tag="PKU88_01605"
     CDS             complement(31098..32102)
                     /gene="amrS"
                     /locus_tag="PKU88_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPX68018.1"
                     /translation="MKEAKHYEKQEGGNVKCRLCPHECNIAPGKYGICRVRNNIGGIL
                     YTHNYGKISSIAMDPMEKKPLYHFFPGRYILSIGTVGCNLRCSFCQNYEIAQRGAAEE
                     GGEDNLYEASEAYLLSLCRKEEDCIGVAYTYNEPSIWFEYILDTAGHIRSEGFKNVLV
                     TNGYISREALSELLPYIDAMNIDVKGFTEKYYRNICGGSLEHVKKTVEAAAAECHVEI
                     TTLVVPGHNDSEREIQELSKWLSSINPSIPLHLSRYFPRYRMKEQPTPAGTLISLKKA
                     AESYLEYVYIGNMQSEAVNTYCPQCGALLIRRTGGIIIEHLDDGICFKCGKAISITGI
                     "
     gene            complement(32118..33491)
                     /gene="amrA"
                     /locus_tag="PKU88_01610"
     CDS             complement(32118..33491)
                     /gene="amrA"
                     /locus_tag="PKU88_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPX68019.1"
                     /translation="MGKIAGAFIFPHPPIMVEEVGGSETKKVSNTVVGALEASERIAE
                     IKPDVIVLITPHGAMLKDAMVIAADERLEGSLARFGAPGTKLSFDNDLELADKIMEQA
                     DKKQAYCIPMHEDVKNTYRYASGIDHGAMVPLYFVSKKYRHFKLVHINYSLLSREQHY
                     KFGIAIRDAVAELDRRAVIIASGDLSHRLTKNAPAGFDPRGEEYDKKYMKIIKEGDVT
                     GLLSMPAGLLEDAGECAYNSTVVLMGCLDGCSVKGEVFSYEGPFGVGYCVAEIHAEAC
                     ADKKKVISSDPYVQLAIDTLETYIKTRRKIDVPEGLPDEMTTRRAGVFVSLKKYGELR
                     GCIGTIKPTTKNIAAEIIQNAISAGTRDPRFLPVEESELSELAYSVDVLGEPEPINTK
                     DELDVKRYGVIVRSGYKSGLLLPNLEGVDTVDYQVSIALQKAGIRPGEPYKMERFEVV
                     RHYQYME"
     gene            33908..35743
                     /locus_tag="PKU88_01615"
     CDS             33908..35743
                     /locus_tag="PKU88_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX68020.1"
                     /translation="MPDLKRIVILGAGYAGVEAAKVLGKKFKKDDSVEITLINDNPFQ
                     TLLTELHEIAGRRTEKESVMVDLYKVFKATKVNFVRDRIESIDYEAKTLTSSKGTYSY
                     DYLVLGCGAEPAYFGIEGVKEYGYTIWSLKDALKIREQVLKSFEAARDEKDAAKRKEL
                     LTFVVAGAGFTGIETVGELFEWKDVLCDEYGIDSKEVTIYNVEAMPTILPILRPSLQA
                     KAASFLTKKGVKLMTNSPIVKATPDGIVLKDGQEIKTKTLIWTCGVQGNSFTASSGLT
                     PGKRNRVQVNDYMQSMDKEEVYLVGDNAYYEIDGKPIPQIVETAIQTGECAAHNIAAD
                     IAKTEKKKFKLNTHGFMVSIGSHYCVAELMGTPLSGFIAMAMKHLVNLHYLWGVGGVR
                     LIWNYLLHEFFHMKNNRAFVGGHLAHRTHTSWLVLLRLYVGVMWFLEGWKKVQEGWLN
                     PANIKIVSVAGTSGSSAAGAAAEAVTSASQAAGAAGEAAAAAGPVPILAEPLGIYQWF
                     QDTFIIANPFFFQAVVVLMEVGIGLALMAGLFTFFASLGSLFLTANFIVSAMAGYDIL
                     WYVFASIALMGGAGGAFGLDYYVQPWIKKAWKKTKFAQASYLYFD"
     regulatory      36220..36353
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            36422..37219
                     /locus_tag="PKU88_01620"
     CDS             36422..37219
                     /locus_tag="PKU88_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HPX68021.1"
                     /translation="MAAAIDINAVVERIDKYHAAFRCYKGLNYGRSVLCMKKNLKSII
                     CVTIICLLLVGQTIGVFADTYYGLLKFGSRGTAVVKLQQALINKGFSPGAADGIYGKM
                     TENAVIKFQQANKLRIDGIAGRETQSRLFAAAAVASRGTSYTSTDLYWLSRIINAEAE
                     AEPYAGKVAVGNVILNRVKSGSFPNKVKGVIFEYYKGIPQFSPVADGTIYNDPNADSI
                     KAAKAAFSGTSYAGNATYFFNPNKSEGTWIVNNKTFVKRIGNHVFYK"
     gene            37353..38042
                     /locus_tag="PKU88_01625"
     CDS             37353..38042
                     /locus_tag="PKU88_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPX68022.1"
                     /translation="MCGCEHRRNHANCIESVPIFGSLSREEMLEIANITSAKTFEKGD
                     MVYMAGDKSNNLYVLHTGRVKISRLGKNGKEQVIRVIGPGEFMGELSLFGSLPLTDNA
                     EVLETSTMCVIEGSKLKELMAKYTSIAFKVMGELSKRLEKAENLIEAISLNSVEQRLA
                     QALLALADRKNEVTLNMSKGDFASQMGMSQETLSRKLAVFQAEGIIELKGHKKIIILN
                     KAGLEEISAAV"
     gene            complement(38039..39490)
                     /locus_tag="PKU88_01630"
     CDS             complement(38039..39490)
                     /locus_tag="PKU88_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPX68023.1"
                     /translation="MNFRLVLRVLGMVLACEALFMLPSLAVSVYFGGDDIAAFALSIL
                     ITAVIGIPLTYIKANKHGMYARDGFAIVALGWILLSFFGSLPFVISGAIPSYIDAFFE
                     ASSGFTTTGASILTQIERLPKGILFWRSFTHWVGGMGVIVLTLAILPSVGAGGLQMMK
                     AESPGPTPGKIVPKVAETAKILYGIYIVITIVEIIALKMAGLPLFDSAIHTFGTVGTG
                     GFSNMNTSVGEYNNVYAEAIIAVFMFICGVNFSLYYQMLKGDLKAAFKDEEFLFYLRT
                     VLIAIILITINLYGNVFSSVWESLRHSSFQVVSIISTTGYVTSNFEEWGMFSKIILFM
                     LMFVGGCAGSTGGAIKNIRFLLLFKVMKRELLQIIHPKAVYSVRLGGRAVNERTISEV
                     LGFFFMYIIIFIAAVLIISLDNLDWATTIGSVAATIGNIGPGFGIVGAVGNYSTLSNL
                     SKIVLSMCMIIGRLEIYPILLLGMPSFWKRTSV"
     gene            complement(39504..40847)
                     /gene="trkA"
                     /locus_tag="PKU88_01635"
     CDS             complement(39504..40847)
                     /gene="trkA"
                     /locus_tag="PKU88_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPX68024.1"
                     /translation="MKIIIIGAGKVGYNLAENLSKSNHDVIIIDKNYAALSKAEENLE
                     VLCIKGSGVSTNILMEAGIETADLLIAVTNSDEVNMVCCLTAKKLGVKHTIARIRDHE
                     YAHELLLLKEELGLDLIINPEQAAADEIANVLNFSSAINVENFAKGRVKMIEIKVAED
                     MPMVGMRLKDIGSKFESSVLVGVVIRDDEVIVPNGDFIINENDSIYLIGQPSHLYNFC
                     KVFGKVPHKIRNVMIVGGGRIAVYLGKLLAEMGMKVKVIEIDREKCIQLSELIPEALI
                     IHGDGTDEELLRSENIDEMDAFIAVTGMDEENMMAALIAKHNGAKKVISKASRTNYIS
                     IIKSLGIDSVISPKQITTNQILKYVRGNSVESLYRIIEGQAEITEIIAEPESKILNTK
                     IKKLGLPKDTIIATIVRKNEVVIPHGNDIIKEGDRVIVISKNKNISSLDEVAAVS"
     gene            complement(40885..41343)
                     /locus_tag="PKU88_01640"
     CDS             complement(40885..41343)
                     /locus_tag="PKU88_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68025.1"
                     /translation="MGSNIRYISSRQQLIDEVETLLNNSQKLDIMVSGVWTKSLYEDI
                     IGLIMDKEFSSTCRVIIPKLIFKSSISLTLLRNLCKAEGQVRVNNSMTNNVLMIGRYA
                     FVLSFSSRLNSFNMLSTTFECSVMTDEVEIVNELKEQFDNIFENSVIFRM"
     gene            complement(41485..42420)
                     /gene="cysK"
                     /locus_tag="PKU88_01645"
     CDS             complement(41485..42420)
                     /gene="cysK"
                     /locus_tag="PKU88_01645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPX68026.1"
                     /translation="MKKVSKSITELIGNTPLLETVNYNSQNGIKGKLIAKLEYFNPGG
                     SVKDRVGYAMIKDAEDRGLVSKDTVIIEPTSGNTGIALALVAAARGYRLILTMPETMS
                     IERRSLLAALGAELVLTPGSDGMKGAIKKAEELVSEIPNAFMPQQFNNPANSAAHRNT
                     TAEEIWRDTEGQADIFVAGVGTGGTVTGVGEVLKGKNPDIRIIAVEPYDSPVLSGGTP
                     GPHKLQGIGAGFIPNVLNASIIDEIIKVKSEEAFAAARKLAAAEGILVGISSGAALHA
                     ATEVAKRPDSAGKNIVVLLPDTGERYISTALFQNA"
     gene            complement(42483..42929)
                     /locus_tag="PKU88_01650"
     CDS             complement(42483..42929)
                     /locus_tag="PKU88_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPX68027.1"
                     /translation="MKISTKGRYGLRAMVDLSVYSAGDHISLKSIAERQNISEGYLEQ
                     VFSTLRKAGLINSIKGAQGGYALADKPANITVGAVLRALEGNLSVIDEKSMEEKTNDK
                     IEECIREKVWSAINSSIGRVIDSITLEDLTNEYRKMTSNEAYMYYI"
     gene            complement(43048..43122)
                     /locus_tag="PKU88_01655"
     tRNA            complement(43048..43122)
                     /locus_tag="PKU88_01655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43087..43089),aa:Glu,seq:ctc)
     gene            43405..44823
                     /locus_tag="PKU88_01660"
     CDS             43405..44823
                     /locus_tag="PKU88_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX68028.1"
                     /translation="MLIHVVKPTETLWQIANRYRVSTASIAEVNGLPDPNRLVVGQAL
                     VIPTEDIFHTVLPGETLWKIAQAYGTTVQAILQNNQIANPDNIYPGLLLYIPALRYRI
                     QPGDTLWSVAQRFGVPLNSLIKINNIENVNLVYPGTVLVIPRKQRPPIDVNGYIYILG
                     QEAVPIIEEAGEHLTYLSPFAYLIREDGSLQAINDIPAIQAAKSYDAIPMMSITNFTS
                     TALGENLAHVVLNSPAVIERLLANIINIMKGKGYSGLNVDFENVLPADREPYNRFLQQ
                     AVTRLHQEGFFVSTAVAPKTSGVQTGLLYEAHDYEAHGRIVDFVILMTYEWGWRKGPP
                     QAISPLNQIRRVLDYAVTVIPREKIFMGFQIYARDWLLPFAWGQEAETFSVQEAVSRA
                     VRYGASIQYDTAAQSPFFRYTDAQGRAHEVWFEDARSTQAKFDTAKNYNLRGISYWAL
                     GYPFPQNWVLLEDNFTIRKGGS"
     gene            45019..46224
                     /locus_tag="PKU88_01665"
     CDS             45019..46224
                     /locus_tag="PKU88_01665"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HPX68029.1"
                     /translation="MSDLNKLKNISNVRTFKENEYIFHEGDTGNDMYILLSGRVSIFI
                     KSKDNLQIKLCDICAGGFFGEMSLLEGEPRTATALTIENTVALSVNKDNFEAFVVEQP
                     SMAFKLLKGLSSRIRALDEELSRLKKGSQYVIPPADSTDALSDSAPCISASNPEDLKG
                     VLYHDEHGTYEGESPDTDTFYIYDKKITCPICGKEFELSVTSQSKLRLNSIDFDFRHR
                     YDGFEPLWYNVLICPFCYFAGYSNEFDNHMDNLCKAFTEKTAPIRKKLAFKYSQPRKL
                     DEVFTAYYLALHSAKALSAGPLKSARIWLQLSWLYKDAGDEKMYAIATDQAFEHYYDV
                     LYGSSLTKLTDEQDQQCCLILGELYLLKNDRNQAVRQFHAAIRRGGGNALYNKQAQDR
                     IYYVKHEMK"
     gene            complement(46334..47068)
                     /gene="cwlD"
                     /locus_tag="PKU88_01670"
     CDS             complement(46334..47068)
                     /gene="cwlD"
                     /locus_tag="PKU88_01670"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HPX68030.1"
                     /translation="MRIYIIDRSKAVGYMIKFAWILVLFISICFIGKSTTAAFSLPIR
                     STVVVIDAGHGGRDPGASGSSGITEEEVNLKIALKLRRLIEQGGGTAIMIREDNSGLY
                     TEGGNRQGTRKSEDLKNRHALINSCGADILISIHLNSFPQSQYFGAQTFYMKNEEKSR
                     KLAEGIQNELIKVLDRGNERKAKATDSMYILKNNNMPGALVECGFLSNREEERLLDDE
                     HYQEKIAWSIFVGIVKYMEEEKKAIE"
     gene            complement(47181..47792)
                     /locus_tag="PKU88_01675"
     CDS             complement(47181..47792)
                     /locus_tag="PKU88_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPX68031.1"
                     /translation="MINVLAIYGSHRRGQNSDMLADKILEGIGSNEASIERIYASSQG
                     IKSCTACERCYELGYCVIKDEMQQVYEAMDKADIVVSSSPVYFYSVSSHMKKLIDRCQ
                     AVWASKYRTEGSLISKKHRIGYIVCTAGEPEGKSRFECAVMVFELLYKCINVEFVGSL
                     LVSDVDNQHVRGREDIRQKAFEAGRLLRESISSIKSHNLHNSI"
     gene            complement(47891..48220)
                     /locus_tag="PKU88_01680"
     CDS             complement(47891..48220)
                     /locus_tag="PKU88_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HPX68032.1"
                     /translation="MNRLGDTEYKSISLPRLNNLKPNIESTALKLMEEAGELAQLIGK
                     YRGLNGEKCRMEERQCVDRIAEELLDVAQVAISMMFVLEESYSIDIQEKVESHIKKLK
                     GKGYIKG"
     gene            complement(48246..48905)
                     /locus_tag="PKU88_01685"
     CDS             complement(48246..48905)
                     /locus_tag="PKU88_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPX68033.1"
                     /translation="MEFRNGQFLVILSPFTDSEAYGYINSITEDSLILSVNPAGFLRN
                     GWGILCIFLENDDIYEFYSEITYLEGNEIIIKKPIENKLNTIEKRRFARVDCEIGFVA
                     RPVTINNVSVGKLGKTFLGTIKNISAGGVLAETNLCLPVDSVFSFKLKTNYFIDCTAK
                     VLRVNELPSEKKFQMGCKFVNMRIEDFKAISMFTFKEQLKKKRKQFYETAAKQKIHIS
                     C"
     gene            complement(49160..49420)
                     /gene="rpsI"
                     /locus_tag="PKU88_01690"
                     /pseudo
     CDS             complement(49160..49420)
                     /gene="rpsI"
                     /locus_tag="PKU88_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(49938..50066)
                     /locus_tag="PKU88_01695"
     CDS             complement(49938..50066)
                     /locus_tag="PKU88_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68034.1"
                     /translation="MLRYEGDETFLSDVIGKSALQPGDRVTLSFSAQDALVLPAQA"
     gene            complement(50050..50502)
                     /locus_tag="PKU88_01700"
                     /pseudo
     CDS             complement(50050..50502)
                     /locus_tag="PKU88_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(50491..50898)
                     /locus_tag="PKU88_01705"
                     /pseudo
     CDS             complement(50491..50898)
                     /locus_tag="PKU88_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(50895..51215)
                     /locus_tag="PKU88_01710"
     CDS             complement(50895..51215)
                     /locus_tag="PKU88_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68035.1"
                     /translation="MEPAPAEEGGKLGGELNLLVMPGYEEEKIIKPFEEKYGVKINAK
                     VYSTSDQMFSLLANSAEGEWDIVTPDTPWVAKLVDADLIDELNPKIIRKLIISMTAGR
                     ILTR"
     gene            complement(51652..52296)
                     /locus_tag="PKU88_01715"
     CDS             complement(51652..52296)
                     /locus_tag="PKU88_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPX68036.1"
                     /translation="MQNCRLFSGLSDRQIKLAKQQLDGVINSYREAEVIVEEDAIVNR
                     IGILLQGNAYISKFTMDGKELLMQKLVPFYLVCAEVAFTRKQNAPYTIYCEKDTDIYW
                     FSIEKITKNGLIDDGIREILLRNIMLYIADENMRKYHKIEAISIKGIRERIVHYLALQ
                     QKINGSNQFYIKFDREKLANFLGMNRSVLSHELKRMEKEGLITFKKNYFEIFRL"
     gene            complement(52497..53534)
                     /locus_tag="PKU88_01720"
     CDS             complement(52497..53534)
                     /locus_tag="PKU88_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX68037.1"
                     /translation="MKRLLALTMAIIMLMVAFSGCAPANSQPDVITDFTVTDMAGRQV
                     TIPGEIKSIATFGSVGVLNAFVELMGSGDLLCNQMPARFTKSDKWKMQYEFAPQIKDA
                     PLLANSNDELLLEEVLKLQPDLCITMTKETAEQLGENGIPCIYLEWKQTEDVKAAVNL
                     MGEILKKEDIAEDYLAYFDSMVQEASDLTAKLTDEDKKTVLYGNVTNLTQPHVIAEWW
                     IDVAGGISVTNDGKTTGQSREYTQEELLLWDPDIIIVTERDMIDKIKEDKLYTDVTAV
                     KAGSIYYIPTVAHTWGNRTVEQPLTVFWTLNKLYPELVSDNMLAEKVSYFYSHFFKYD
                     LTPQQISEIIE"
     gene            complement(53582..54373)
                     /locus_tag="PKU88_01725"
     CDS             complement(53582..54373)
                     /locus_tag="PKU88_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68038.1"
                     /translation="MIRVENVIFSYRHSAPVLRDVSFNFERGESLCVLGPNGTGKTTL
                     FRCLLNLHSIQGGKIFIDGKDAASLRPRVRARLISYVPQASALTFPYTAWETVLMGRV
                     AHQKLGASVSLKDRIAAEAAMERLNVLCMAQCRFHELSGGERQLVLIARALAQETDCL
                     ILDEPTASLDYANQIRILQVIRQLAEDGYAILMTSHLPDQAFLAATRVLMLKDGIVFA
                     DGTPEDIITSRNLSSLYGLDVYVADAQAVFNSGTQESKVCIPSMS"
     gene            complement(54375..55391)
                     /locus_tag="PKU88_01730"
     CDS             complement(54375..55391)
                     /locus_tag="PKU88_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPX68039.1"
                     /translation="MKKQIRYPLMMVMFLCGLVAAFFISFLIGRYPIAIRTVADIILS
                     RFFTITAYWEGTLEKVVLHVRLPRILLAILVGGSLSMAGASYQTLFKNPMVSPDILGV
                     SAGAGFGAALAMLQNGSWAGIQFFALTFGLLAVGLAYLIASLSGGRQNLTVLILAGIV
                     TSSLFQSLLSVVKTLADTDNQLPSITFWLMGSLGKGNNDDVLIMLPVVTASAFVLFLF
                     RSHIDALSAGEDEALSMGVNVPLVKIVVIVSSTLLTVVSVSICGIIGWVGMVVPHIAR
                     ALTGASYGRLCSLSFLIGALFLLLIDNIIRGIPGVELPLGVMTALIGTPAFAVMLARI
                     KRGW"
     gene            complement(55388..56224)
                     /locus_tag="PKU88_01735"
     CDS             complement(55388..56224)
                     /locus_tag="PKU88_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPX68040.1"
                     /translation="MSKNEILLESVHIVKGRLKSELSNITIDRAVLGLFFSGVKLSTG
                     HGGLCFTPVKDIPAAVCCPSSAAAMPLSGKLRGRSAEQYLEDVFSDNILKKTLGIAAL
                     NALSNLIWELEPPEGYSIAIGMDAFDNVDVSQYKKSVVIGALVPMLKKLITGGCDFRV
                     LEQDPRTLKSGELPYYAPAEDAHKYVPDADLLVITGVTILNDTLPGILNMAKPGAQII
                     VTGPTASMLPDALFSRGVTMVGGILVTKADELLDIISEGGSGYHFFGKSAERTVIFKN
                     QP"
     gene            complement(56305..57504)
                     /locus_tag="PKU88_01740"
     CDS             complement(56305..57504)
                     /locus_tag="PKU88_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPX68041.1"
                     /translation="MYLNQKQFLMRSEALKELFGRWNPEIATESIAVSEAVGRVLSDA
                     VSSVNTLPVCRSSKLDGIAVSFEALMQASGGQFPWTGTIAHVSADTGDDFDDAFDTVI
                     AVERIVRHSDGSLTITSKEPIKQGQNIRPRGDTLKEGQQLLKKDTLLQPVHLAFLSTA
                     GVSTVKVYKKPVVSFIPTGNELIPIGVSPKRGQNIESNSLMVRTMLAQWGAKAHCCPI
                     VKDDNVLMEETLLNACSKADIVLINGGSSMGSEDYASRLLQKHAEWFQHGIRCIPGIP
                     IALAIIDKKPVINLPGPTLASYYAMDWCVKAMVFHMLKQDMPLRRTVRAVLKQKVQKP
                     SHLEMYVRLRVKCINGRYTAEVLSPQKGLAQLLADCNALFIAPIGKETFEAGEMVRAE
                     LLSGFDI"
     gene            complement(57772..58167)
                     /gene="rpsI"
                     /locus_tag="PKU88_01745"
     CDS             complement(57772..58167)
                     /gene="rpsI"
                     /locus_tag="PKU88_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPX68042.1"
                     /translation="MAKKVQYFGTGRRKQSVARVRLVPGEGKIIINDREIDDYFGLET
                     LKLIVRQPLILTDTLSKFNVLVNVIGGGFTGQAGAIRHGISRALLEADDTLRPMLKKA
                     GFLTRDPRMKERKKYGLKKARRAPQFSKR"
     gene            complement(58189..58623)
                     /gene="rplM"
                     /locus_tag="PKU88_01750"
     CDS             complement(58189..58623)
                     /gene="rplM"
                     /locus_tag="PKU88_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPX68043.1"
                     /translation="MKSYIPKTDEIQRKWYVVDATDKVLGRLASEVAKILTGKNKPIY
                     TPHIDTGDYVIVINCDKVVLTGKKLDQKMHRTVTTRVGSMKETPYRKFMAEQSDKAVY
                     EAVKGMLPKNSLGRKMLKKLRVYKGAEHENAAQMPEALELDI"
     gene            complement(58848..59597)
                     /gene="truA"
                     /locus_tag="PKU88_01755"
     CDS             complement(58848..59597)
                     /gene="truA"
                     /locus_tag="PKU88_01755"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPX68044.1"
                     /translation="MRNIKLIIEYDGTNYCGWQVQENGPSIQAEIEKALLSVTGEAIS
                     INGSGRTDAGVHARGQAASFITESSIPPEKFAYALNNKLPEDIVIKESGEVPLDFHAR
                     FSAVGKKYSYLVINSRFPSALLRNHAYHVNYCERLDIDKVKTAAEAFIGTYDFSGFMA
                     AGSKVSNTVRTIYDISIEQEGELIRFIYKGSGFLYNMIRIITGTLLYAGIGKINPEDM
                     KDVILSKNREQAGITVPARGLYLEEVYYNLS"
     gene            complement(59661..60452)
                     /locus_tag="PKU88_01760"
     CDS             complement(59661..60452)
                     /locus_tag="PKU88_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPX68045.1"
                     /translation="MIKDITIGQYIPGDTAVHNLDPRIKIIITFVFITLLFLVGNYFS
                     YLYIIAFMGLAIGLSKISLKFIAKGLKPLIVIIVLTVILNAFMTQGEVIYRIWGLSIT
                     KEGLYQAGFMGLRLIFLITGASLLTLTTSPIALTDGIEKLLNPLKRIGVPAHELAMMM
                     TIALRFIPTLLEETDKIMKAQMARGADFETGNLINRAKAMVPLLVPLFISAFRRADEL
                     ALAMEARCYRGGEKRTRMKQLKLEFRDYSAMMIIAVFCFLVIWSR"
     gene            complement(60459..61307)
                     /locus_tag="PKU88_01765"
     CDS             complement(60459..61307)
                     /locus_tag="PKU88_01765"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPX68046.1"
                     /translation="MPVKVENLTHIYMKDSVFEHVAIDNISFEIQDGEFIGLIGHTGS
                     GKSTLIQHLNGLLKPSSGKVFIDGEELGSKEVQMKKIRQKVGLVFQYPEYQLFEETVY
                     KDVAFGPRNLGLSEDEVDARVRKAIELVGLDFDSVSNVSPFELSGGQKRRVAIAGVLA
                     MEPKVLILDEPAAGLDPKGRDDILKGFKTLHETQKITVVLVSHSMEDIANLVDKVLVM
                     NKGKIAYFDTPRNVFGEAETLEKIGLGVPQMTYLVRELRKKGYPIADCINIDEAKNEL
                     LKLFKR"
     gene            complement(61292..62131)
                     /locus_tag="PKU88_01770"
     CDS             complement(61292..62131)
                     /locus_tag="PKU88_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPX68047.1"
                     /translation="MANMITTENVRFEYGTNSGSAKPALDDININIAKGEFVAILGHN
                     GSGKSTLAKHFNALLLPGAGTVYVKEMNTKNEKLLWDIRQTAGMVFQNPDNQIVATIV
                     EEDVAFGPENLGIPPLEIRRRVDDSIKTVDMTEYAKHSPHLLSGGQKQRVAIAGIIAM
                     KPECIILDEPTALLDPVGRKEVLNTIIKLNKEEGITIILITHYMDEAIQADRIYVMKN
                     GKIIMEGKPKDVFKEVETVKAAGLDVPQVTELSHRLNREGLQLSPDILTVEEMVVELC
                     RLK"
     gene            complement(62324..62665)
                     /gene="rplQ"
                     /locus_tag="PKU88_01775"
     CDS             complement(62324..62665)
                     /gene="rplQ"
                     /locus_tag="PKU88_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPX68048.1"
                     /translation="MAGYRKLGRPSDHRKAMLRNLVTSFLEHGKIVTTETRAKETQSI
                     AEKMITLGKRGDLHARRQALGYITKEDVVKKLFDDIAPKYKERNGGYTRIAKVAPRRG
                     DAAEVVVLELV"
     gene            complement(62733..63680)
                     /locus_tag="PKU88_01780"
     CDS             complement(62733..63680)
                     /locus_tag="PKU88_01780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPX68049.1"
                     /translation="MIEIEKPKIECIEMSEDYTFGKFVVEPLERGYGTTLGNSLRRVL
                     ISSLPGVAVTSIKIDGVLHEFSTIPGVAEDVTEIILNIKNLALKMHGENTKIIKIDVQ
                     GEKEIKASDIICDADVEIINKDLHIATVDEGHRLYMEMTVEMGRGYQISEKNKQPGQP
                     IGIIPIDSIFTPVRKVNYLVENTRVGQVTDFDKLVLEVWTDGTINPDEAISFGAKILS
                     EHLKLFITLTEHVGNVEIMVEKEEDKKEKVLEMTIEELDLSVRSFNCLKRAGINTVEE
                     LTQRTEEDMMKVRNLGKKSLEEVQQKLEALGLSLKTSED"
     gene            complement(63790..64416)
                     /gene="rpsD"
                     /locus_tag="PKU88_01785"
     CDS             complement(63790..64416)
                     /gene="rpsD"
                     /locus_tag="PKU88_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPX68050.1"
                     /translation="MARYTDAKCRLCRREGSKLYLKGDRCYTDKCAVARRAYAPGQHG
                     QGRKKVSEYGIQLREKQKVRRIYGILEKQFRDYFVEAERQKGVTGENLLRLLELRLDN
                     VAYRMGFGESRAEARQLVRHGHFTVNGRKVDIPSYQTGLNDLIAVKPGSKSTDKFKGL
                     AENAAGKTAPQWLSINTEMMEARIVALPAREDIDLPIEEHLIVELYSR"
     gene            complement(64437..64835)
                     /gene="rpsK"
                     /locus_tag="PKU88_01790"
     CDS             complement(64437..64835)
                     /gene="rpsK"
                     /locus_tag="PKU88_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPX68051.1"
                     /translation="MVTKPKAKKTRRRKERKNVERGAAHIQSTFNNTIVTLTDSTGNA
                     LSWSSAGSLGFRGSKKSTPFAAQMAAEVAAKGAMEHGLKIIEVFVKGPGAGREAAIRS
                     LQAAGLEVSLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(64862..65230)
                     /gene="rpsM"
                     /locus_tag="PKU88_01795"
     CDS             complement(64862..65230)
                     /gene="rpsM"
                     /locus_tag="PKU88_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPX68052.1"
                     /translation="MARIAGVDLPRDKRAEIGLTYIFGIGRATSNRILAEAGVSPDTR
                     IKDLTEAEIGRLREVIDRSVKVEGDLKRDIALNIKRLMEIGCYRGLRHRRGMPVRGQR
                     TKTNARTRKGPKKTVGVRRK"
     gene            complement(65324..65437)
                     /gene="rpmJ"
                     /locus_tag="PKU88_01800"
     CDS             complement(65324..65437)
                     /gene="rpmJ"
                     /locus_tag="PKU88_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPX68053.1"
                     /translation="MKVRPSVKPICEKCKIIRRKGKVMVICENPRHKQKQG"
     gene            complement(65455..65673)
                     /gene="infA"
                     /locus_tag="PKU88_01805"
     CDS             complement(65455..65673)
                     /gene="infA"
                     /locus_tag="PKU88_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPX68054.1"
                     /translation="MSKQDVIEVEGKVVEALPNAMFQVELENGHKILAHISGKLRMNF
                     IKILPGDKVTIELSPYDLTRGRITWRGK"
     gene            complement(65702..65965)
                     /locus_tag="PKU88_01810"
     CDS             complement(65702..65965)
                     /locus_tag="PKU88_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPX68055.1"
                     /translation="MQDISLGQIVISAAGRDKGCKLVVLCIIDEKYVYISDGDIRKVD
                     KPKLKKVKHLKKLNHVAENIKEKLESCEKLSNSEIRKLLKSID"
     gene            complement(65985..66731)
                     /gene="map"
                     /locus_tag="PKU88_01815"
     CDS             complement(65985..66731)
                     /gene="map"
                     /locus_tag="PKU88_01815"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPX68056.1"
                     /translation="MISIKSPREIELMRIAGRIVAETHELLSKAIRPGITTLELDTIA
                     EEFIRKRGAIPAFKDYNGFPASICSSINEQVVHGIPGPTALKDGDIIGIDIGAVYDGY
                     YGDAARTYWIGSIDKETERLLKVTEESFFKGIEYALPGNRLSDISHSIQKHVESNGFS
                     VVRDFVGHGIGRNMHEDPQIPNYGLPHKGPRLAAGMALAIEPMVNQGKYAVKVREDGW
                     TVVTADGKPSAHYENTIVITNGKPEILTLL"
     gene            complement(66728..67378)
                     /locus_tag="PKU88_01820"
     CDS             complement(66728..67378)
                     /locus_tag="PKU88_01820"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPX68057.1"
                     /translation="MRIVLLGSPGSGKGTQASFIEKEFGIPHISTGDIFRDNISRNTA
                     IGAEAKKFIERGLLVPDSLTVSIVESRLLEEDCNEGFLLDGFPRNIAQAEALDKDLEK
                     MGKKLDAVVNLEVSAETIIERMAGRRICSDCGESYNLCFLKPAADGICDKCGGKLYIR
                     EDDKPETVMNRLNVYKNQTFPLIEYYKGKGILISVNGEQPADKVYGDICRVLERFK"
     gene            complement(67442..68698)
                     /gene="secY"
                     /locus_tag="PKU88_01825"
     CDS             complement(67442..68698)
                     /gene="secY"
                     /locus_tag="PKU88_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPX68058.1"
                     /translation="MFNTLRNAWKIPDLRQKMLFTLVMLIIFRLGSFIPVPGMNTEAL
                     SRLIQSGGLLGFFNTISGGAFDNFAIFAMSITPYINSTIIMQLLTIAIPSLEALAKEG
                     EEGRRKIAQYTRYGTVVLALIQAAALSYGLRGYLLRTDAASVILVALSLTAGTAFLMW
                     LGEQINEKGIGNGISLLIFAGIISRVPGGALKIFELYKVKSTNLIEIAFFLIFAILII
                     MAVIFIQEGERRIPVQYAKRVVGRRMYGGQSTHIPLKVNQAGVIPVIFAMSLIAFPGQ
                     IAQFFPNSWAYRFFNTYLGWGSWAHSILYALLIIGFTYFYTAVSFNPIEVANNIKKYG
                     GFIPGIRPGKPTSDFITKSLNRITLVGAFFLAVIAIVPIFLMNFTGIKVYFGSTSLLI
                     VVGVAMDFVKQVEAQMVMRNYQGFLK"
     gene            complement(68700..69140)
                     /gene="rplO"
                     /locus_tag="PKU88_01830"
     CDS             complement(68700..69140)
                     /gene="rplO"
                     /locus_tag="PKU88_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPX68059.1"
                     /translation="MKLHELKPAEGTTKSKKRLGRGTATGQGKTAGKGQKGQKSRSGG
                     GVRVGFEGGQMPLYRRLPKIGFTNVFRKEYAVVNLSDLERFDNGTIVNPEVLKEAGLV
                     KAMLAGVKILGNGDLTKNLTVQAHKFSKTAAEKIAAAGGKVEVI"
     gene            complement(69157..69336)
                     /gene="rpmD"
                     /locus_tag="PKU88_01835"
     CDS             complement(69157..69336)
                     /gene="rpmD"
                     /locus_tag="PKU88_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPX68060.1"
                     /translation="MAKLKVKLIKSTIGRKDDQIATVKALGLKKINSEVEHNDTPQIR
                     GMIQKVIHLVEVKEM"
     gene            complement(69349..69849)
                     /gene="rpsE"
                     /locus_tag="PKU88_01840"
     CDS             complement(69349..69849)
                     /gene="rpsE"
                     /locus_tag="PKU88_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPX68061.1"
                     /translation="MQPIDSSKLDLKEKVVAINRVAKVVKGGRNFRFSAIVVVGDMNG
                     YVGVGTGKSVEIPEAIRKGIEDAKKNLIKVSIVGTTIPHEVIGVAGAGRVLLMPAKEG
                     TGVIAGGPARAVLELAGIKDIRTKSLRSNNPRNMVNATIQGLASLKTVEDVAKLRGKA
                     VEEIIG"
     gene            complement(69868..70236)
                     /gene="rplR"
                     /locus_tag="PKU88_01845"
     CDS             complement(69868..70236)
                     /gene="rplR"
                     /locus_tag="PKU88_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPX68062.1"
                     /translation="MITKASKNSTRQKRHLRMRKKVFGTSERPRLNVYRSLNNIYAQI
                     IDDTKGVTLVSASTLDKELKDNPGHGGNKESAKAVGTLVAKKALDAGIKQVVFDRGGY
                     IYHGRVKELADAAREAGLEF"
     gene            complement(70258..70800)
                     /gene="rplF"
                     /locus_tag="PKU88_01850"
     CDS             complement(70258..70800)
                     /gene="rplF"
                     /locus_tag="PKU88_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPX68063.1"
                     /translation="MSRIGKLPIEIPQGVEVKIADDNTVSVKGPKGQLIKKMHEDINI
                     EKKDGKLLVTRPTDQKMHRALHGLTRALLNNMVEGVSKGFEKVLEINGVGYRAAKSGK
                     KLTLTMGYSHPVEMDDPEGITTEVPAPNKIIVRGADKEKVGTHAANIRAVREPEPYLG
                     KGIKYSDEVIRRKEGKAGKK"
     gene            complement(70828..71226)
                     /gene="rpsH"
                     /locus_tag="PKU88_01855"
     CDS             complement(70828..71226)
                     /gene="rpsH"
                     /locus_tag="PKU88_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPX68064.1"
                     /translation="MVTTDPIADMLTRIRNANTVKHETVDIPASNMKKAIAQILLDEG
                     YIKGYEIIGDGSTNSIRISLKYGSNKQKVITGLKRISKPGLKVYAKNEDIPKVLGGLG
                     IAIISTSKGIMTDKNARLQGVGGEVLCYVW"
     gene            complement(71407..71592)
                     /locus_tag="PKU88_01860"
     CDS             complement(71407..71592)
                     /locus_tag="PKU88_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPX68065.1"
                     /translation="MAKKSMVLKQQRTPKYKTRAYTRCKICGRPHAYLRKFGICRICF
                     RELAYKGQIPGVKKASW"
     gene            complement(71622..72161)
                     /gene="rplE"
                     /locus_tag="PKU88_01865"
     CDS             complement(71622..72161)
                     /gene="rplE"
                     /locus_tag="PKU88_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPX68066.1"
                     /translation="MARLKEKYLNEVAPAMMQKFQYKSVMQIPKLEKVVINMGLGDTK
                     DNAKGLDSAVSELGTITGQKPVITKAKKSVAAFKVRQGMSVGAKVTLRGNQMYEFLDK
                     LFNIALPRVRDFRGVSVNSFDGRGNYSLGIKEQLIFPEIEYDKVDKIRGMDIIFVTTA
                     ETDEEARELLRLLGMPYAS"
     gene            complement(72185..72523)
                     /gene="rplX"
                     /locus_tag="PKU88_01870"
     CDS             complement(72185..72523)
                     /gene="rplX"
                     /locus_tag="PKU88_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPX68067.1"
                     /translation="MANAEVKKLHVKKGDTVVVISGKDKGKKAKVLIALPQEDKVLVE
                     GVNMSVKHKKPGKNMQQGGIIHQESPIFSSKVMLWCDKCKKGVRLGHRILDDKSKVRY
                     CKSCGEILDK"
     gene            complement(72538..72906)
                     /gene="rplN"
                     /locus_tag="PKU88_01875"
     CDS             complement(72538..72906)
                     /gene="rplN"
                     /locus_tag="PKU88_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPX68068.1"
                     /translation="MIQQETRLNVADNSGAKELLCIRVVGGSTRRYGNIGDVIVATVK
                     DATPGGVVKKGEVVKAVIVRSVKGKRRPDGSYIRFDDNAAVIIKEDKQPRGTRIFGPV
                     ARELREKDFMKIISLAPEVL"
     gene            complement(72929..73183)
                     /gene="rpsQ"
                     /locus_tag="PKU88_01880"
     CDS             complement(72929..73183)
                     /gene="rpsQ"
                     /locus_tag="PKU88_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPX68069.1"
                     /translation="MDRNNRKTRIGKVVSDKMDKTIVVGIETLVRHPLYGKSIKRTTK
                     FKAHDENNECKIGDKVKIMETRPLSKDKRWRLVEIIEKAK"
     gene            complement(73203..73409)
                     /gene="rpmC"
                     /locus_tag="PKU88_01885"
     CDS             complement(73203..73409)
                     /gene="rpmC"
                     /locus_tag="PKU88_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPX68070.1"
                     /translation="MKANKFRDMSVKELEQKLNELKGELFNLRFQMATGQLENPMRVK
                     DVKKSIARIKTILKENELKSVQAK"
     gene            complement(73399..73842)
                     /gene="rplP"
                     /locus_tag="PKU88_01890"
     CDS             complement(73399..73842)
                     /gene="rplP"
                     /locus_tag="PKU88_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPX68071.1"
                     /translation="MLIPKRVKHRKQMRGSMSGKATRGNFIAYGEFGLQATEPGWITN
                     NQIEAARQAMTRYTKRGGKIWIKIFPDKPVTQKPAETRMGSGKGAPEYWVSVVKPGRV
                     LFEVAGVEEEIAREALRLAQNKLPIKCKFVTRRETEEMVGDTNES"
     gene            complement(73842..74540)
                     /gene="rpsC"
                     /locus_tag="PKU88_01895"
     CDS             complement(73842..74540)
                     /gene="rpsC"
                     /locus_tag="PKU88_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPX68072.1"
                     /translation="MGQKVNPHGLRVGIIKDWDTKWYADDKSFADFLVEDNKIREFLK
                     TKLFTSGISRVEIERTAGKIKINIHVAKPGMVIGKGGAGIEELKKQLEKLTNNKGVTV
                     NITEIKVPELDAQLVAENIAAQLEKRISFRRAMKQSMQRTMRVGAKGIKTMCAGRLGG
                     AEIARSEWYREGTIPLQTLRANIDYGFAEAKTTYGRIGVKVWIYKGEVLPQAKTRKSE
                     QAKLNEAAAEGGEA"
     gene            complement(74557..74892)
                     /gene="rplV"
                     /locus_tag="PKU88_01900"
     CDS             complement(74557..74892)
                     /gene="rplV"
                     /locus_tag="PKU88_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPX68073.1"
                     /translation="MEARAIAKYIRISSRKVNIVIDLIRGKKVSEAFAILKHTPKAAS
                     VLLEKLLKSAVANAENNYEMNVDKLYISEIYATQGPTLKRFRAASHGRGVRILKRTSH
                     ITLAVKEKE"
     gene            complement(74937..75221)
                     /gene="rpsS"
                     /locus_tag="PKU88_01905"
     CDS             complement(74937..75221)
                     /gene="rpsS"
                     /locus_tag="PKU88_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPX68074.1"
                     /translation="MSRSTKKGPYVDEKLLKKIIDMNKTNKKVVLKTWSRSSTIFPEM
                     VGHTIAVYDGRKHVPVYMTEDMVGHKLGEFAPTRTFRGHGHHTERSSALK"
     gene            complement(75243..76076)
                     /gene="rplB"
                     /locus_tag="PKU88_01910"
     CDS             complement(75243..76076)
                     /gene="rplB"
                     /locus_tag="PKU88_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPX68075.1"
                     /translation="MAIKKYNPTSPARRNMTVSTFEEITKTEPEKSLVEILKSKAGRN
                     NYGKITVRHQGGGHKRQYRIIDFKRDKVDIPAKVAAIEYDPNRSANIALLYYADGEKR
                     YIIAPNGLKVGDTIISSENADIKVGNALPLKSIPIGTVIHNIELKAGKGAQLVRAAGN
                     SAQLMAKEGEYAQVRLPSGEVRMIRIECKATIGQVGNLDHENISIGSAGRKRHMGIRP
                     TVRGSVMNPADHPHGGGEGRSPIGRPGPVTPWGKPTLGYKTRNNKKASSKFIVKRRNE
                     K"
     gene            complement(76091..76378)
                     /gene="rplW"
                     /locus_tag="PKU88_01915"
     CDS             complement(76091..76378)
                     /gene="rplW"
                     /locus_tag="PKU88_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPX68076.1"
                     /translation="MNAYDIVRKPVITEKSMSEMADKKYTFIVDVNANKTEIKNAVEE
                     IFKVKVDKVNTVKVLGKMKRMGKYEGRRPGYKKAIIKLTKDSKGIEFFEGL"
     gene            complement(76378..77001)
                     /gene="rplD"
                     /locus_tag="PKU88_01920"
     CDS             complement(76378..77001)
                     /gene="rplD"
                     /locus_tag="PKU88_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPX68077.1"
                     /translation="MPNVALYNISGKQVGEIELNENIFGVNVNVEAMQQVVKMYLANQ
                     RQGTQSALTRAEVRGGGIKPWRQKGTGRARHGSIRSPQWKKGGIVFAPKPRSYRYTVP
                     KKIKRIAMKSALSSKANENSIVVIEELNFDAPKTKQVVSLLENLKIDSKTLIVLADNN
                     ENVVKSARNIDGVKATFVNTLNVYDILKYDKFVITKDAVKKVEEVYA"
     gene            complement(77034..77663)
                     /gene="rplC"
                     /locus_tag="PKU88_01925"
     CDS             complement(77034..77663)
                     /gene="rplC"
                     /locus_tag="PKU88_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPX68078.1"
                     /translation="MKKAILGLKVGMTQIFDNEGKAVPVTVIAANPNIVVQKKTVENE
                     GYEAIQVGFDDKAEKKVTKPLKGHFAKAKVATKRYLREFRLEDAASYEVGQEIKVDIF
                     KEGDRVDISGVSKGKGFAGAIKRHNFHRGPMKHGSKYHRWAGSMGASSSPSRTIPGKK
                     LPGHMGAENTTVVNLEVVKVDPEKNIILVKGGIPGIRGSLVMIKDTVKA"
     gene            complement(77815..78123)
                     /gene="rpsJ"
                     /locus_tag="PKU88_01930"
     CDS             complement(77815..78123)
                     /gene="rpsJ"
                     /locus_tag="PKU88_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPX68079.1"
                     /translation="MAKQKIRIRLKAYDHILIDQSAERIVETAKRTGAKVSGPVPLPT
                     EKEIVTILRAVHKYKDSREQFEMRTHKRLIDILNPTPKTVDSLMRLDLPAGVDIEIKL
                     "
BASE COUNT        20691 a        19052 c        13840 g        25041 t
ORIGIN      
        1 ccccataagg cttgcgaaac agctttacca gttcataaac catattaaga ttatgaacgc
       61 cgaacaatgt aggttcagct gccagaatac aatagtccgc atccctgata ctctccatta
      121 ctatacaggc actgcccgga gggcaatcaa taaaggtagt cattcctttc acttttctgt
      181 tttcatccag cagcttctta atcaggggga tgcccgaagc ttcaccggta ttgagtattc
      241 ctgtatttac agtcacttga cctgaaacac ccttctgaat tttccctata attttctctt
      301 tctcattcag cgccttttcc gggcaaagca gaatacaccc gccgcaggaa tgacaaacct
      361 catcaaaaac taccagtctg tcttttatat aagcaagagc attgaatttg caaaagtcaa
      421 cgcaaacccg gcatccgctg cattgctttt catctgcaac agggatttta attgaaacct
      481 cttcctcata aaaattctcc ggcttgaaaa aaagatgacc attgggctct tccacatcgc
      541 aatccaaata aacagatttc ccagccgctg cagccaaatt caccgatatc agtgttttcc
      601 ctgtgcctcc cttgccgctt aacacagcaa tcttcatgtt atttttctcc atggccgtga
      661 aatccggcat gtatttcttc aagcaaagac aattttcctt cagtaaatgc attaacatta
      721 tctttggcag aaccgggtat agtcttatac attttaatgt ttgcagcttt tataacctcg
      781 gctgcgtttt caccgcatcg gggtgtcagc aatatatcag ctttatgatc tacaaggaat
      841 tgtgccgctt tgataccggc acctccctgg ctggctgctg cgctgttttc aaagaacaga
      901 cttcgatttg attcagtgtc gtgaatcaga aaatatggtg tgcgtccaaa ggatatgcat
      961 acacttgctt ccatattttt atcgtctaca ggtattgcaa ttttcataaa aatcctccat
     1021 tctatttact gccgaatctt tgcctgcggc atccgcctct cccgcaggca ttgccgctgc
     1081 cgtcacaaag cctgtattct ccgccttcaa tccacaacac ctttccattt accagtgact
     1141 ctgcaagttt ttttcttgcg tcgacataaa tcccttgaac ggttgtgcgt gcaatattca
     1201 tccggctggc acattcttcc tgtgtaaatc cctccagatc aatcagcctt atggtttcat
     1261 attcgtcaac ggtcataaca atgtaattcg attcgtctgc cggggacccc agagggccga
     1321 agcggttgct ttcaggcagg cagcatactt ttctccattt tcttggtctt ggcatgtgat
     1381 tcacctctct taacagcaga aataactgga gcgtcctccg gttatttctg cttctggcta
     1441 tatattctcc aactgttcat taacgatttt gagccggctt tccagcaagt ccttttgtgt
     1501 ttgcagcatc tccttttgtg tttttgcgga agcccagttt acagcaaagc cttttccgta
     1561 acctcttaca aaaccacgcc tgcatccaaa tcccaatccg gctgcattaa ctccgctgca
     1621 aaagcctaaa cctcttcccg tagctgctcc ggctcccatc ggacctgttc catctcttcc
     1681 tggcatatga ttcacctcca aatagtttat gacatatgtc gctttcaatt tttattatac
     1741 tatgtttctg acatatgtca ataactaatt tgaattagat ttccttatcc aactcgttat
     1801 tataccggta tactgcctct aatcctgtaa cctttgaagc tatttcggtc aaggggatta
     1861 tatcgcttct gtcaatatat tccagagaga atttcctgtt cagtgccata agctgcttta
     1921 gccctgcagc tatcctgttt atgtatgaat ataaccctac cgctccggtt gaaatattca
     1981 aagcatcttc cccatacagc agtttaagct ctctaatgtc tccgaatacc tcttccttag
     2041 cagtgccaaa cctcagatat tctttgggag tcattccttc cttcattaaa tctcctatct
     2101 gcttgcctgc catggctgct gccattgccg cccttcctat gccgacaata tttatatatg
     2161 gggcacctaa cgccaatcct ttatatacct gatcctccat ggcaaagcca ccggttatcg
     2221 caacctgcgg aagattatag ttcttctcct tcattctctt taaaatgtta tacaacaggc
     2281 tctccaagta tactgtcgga attccccatt cattcatcat cttgacgggg ctgttccctg
     2341 atcctcctcc ggctccgtca aaggttacca aatccactcc tgcctttgaa gctatcttaa
     2401 gaatccttat taaatcctta gggtcaaagg ggcctgtctt gaagcatatg cgctcagctc
     2461 caagctttct taattctttt acccgattaa tcagtatatc ttcgttccac ataggaagct
     2521 tgcctatttt ctcaaatacc tgtccttttc cctctttata attttttgct atttcaggat
     2581 ccgacgggtc agggtaaact aagtagccct tcttctggta ttcaagagca gcatctatat
     2641 ccttaactct ccccattcct tgaattccct tggctgcctg tccgaacttc agttcgacgg
     2701 tttttacacc aagttcttta attgcataat ccaatacgcc gaggttttca tcatcgtaat
     2761 tcccctggag tattatgtct ccaaatcctc tgtaatactg cctgaaggat gataccattt
     2821 ccttcagcag cggagagccg gcgaccttcc cattttccag taccagttcc ttatctttcg
     2881 ccacagcatc ttctccaata acaaccagca cccctgacaa tgcagctcct gcataataat
     2941 ccttccagtt cagcttggcc atggcaggaa gaattatagg cgcctttaat ttaattttgt
     3001 tgttgattcc gaaatccaca gcaatattgg ccttgggaaa tgttgcttca tttgcgtcct
     3061 ccgggcagcc gagcgcgcca aagaccctgc cgttgatatt aaagtgggaa aaatccagag
     3121 gatagctttt ttcagaggca aactggtttt tgtctgtttc aaagggatat atggcctctg
     3181 aacctctgac agcagacagg cctatttcac aagttccgat gcagcttgca gtacataccg
     3241 aacacattcc cgagaaatcg gaaatatgct ccggagtcct catttttgta ttggttaaag
     3301 aacttcctaa ggatggacta tatgacatag aaaacccccc ttaattattt ttaagttaat
     3361 tccttcaatt tcaattataa ggtatttatg acatatgtca attattattt ccgtcctctg
     3421 ccagtagtat atatccgacc tgtcccgcaa aaaagtacac ctccaaatga tagatttttt
     3481 atctgacatt tggggtgtac ttcacttttc tggacttcat gtacttctat tcaaactcta
     3541 tggttgccgg tggtttgctt gttatatcat acattacgcg gttaacatgc tccacttcgt
     3601 ttactatcct gttggatatt ttctcaagta cttcataggg tatccttgcc caatcggcag
     3661 tcatggcatc ggagctggta acggcacgga ttgccaccgt atgggaatag gttcgctcgt
     3721 cccccattac ccctacgctt cttatgtcgg gcagggcagt aaagtactgc catatgcatc
     3781 tttccaatcc tgcattcttt atctcttccc tgagaattgc atcgctttcc tttactatgt
     3841 gaagcttttc ctttgttatt tctcccaata ctcttatgcc aagtcccggt cccgggaagg
     3901 gctgcctcca taccaaatcc tccggaaggc caagctccag gccaaccagc cttacctcat
     3961 ccttgaagag ttcccttaaa ggctcaataa gctttaaccg catgttttca ggcaggccgc
     4021 ccacattatg atgggacttt attacagccg cagtcttggt tccgctttca ataacatcag
     4081 ggtaaattgt accttgcacc aggaagtcaa tctctccaag tttatttgcc tcttcctcaa
     4141 aaaccctgat gaattcttcg cctattatct ttctttttct ctcgggttcc gatactccct
     4201 tcagtttgtc aaggaatctg tcctgggcgt taatcttaat aaggttgatg tcaaaacgtt
     4261 tcctgaatac ttcttctact tgctccgctt cattttttct cagcaagccg tggtcgacaa
     4321 atatgcaggt caggcgattt cctatggcct tatgcaccaa tactgccgct actgaggaat
     4381 caacaccgcc gcttaaggcg catattacct tcttgtctcc ggcaagttct tttatccgct
     4441 ctacattttc atcaatgaaa tttcccattg tccaatcccc tgaaagcccg catatatcat
     4501 aaaggaaatt cctcaatatc tgtttcccat actcagtatg ctctacttcc gggtggaact
     4561 gaactccgta caacttcccg gacttgcttc ccatggccgc tacagggcat gtctccgtgg
     4621 ctccaataat gtcaaatccg gccggagggc tgtctacata aacggtatgg ctcatccagc
     4681 atatactgtc ttgtggtact tctctgaaaa gagtattctc cagaagcttc acctgcgtct
     4741 ttccgtattc cctcttaaca gcccttgcaa cctttcctcc cagcatatgt gccataagct
     4801 gcgctccata gcagatgccc agaataggta tccccatttc aaacattccc ggatcgcact
     4861 tgggagcctc ctcttcatat acacttgcag gcccgccggt gaatattatc cccttgggat
     4921 tcttcttcct tatttcatcc aggctgtaat cataaggcac aacttcacaa tatacattgg
     4981 cctctcttac tcttcgggca ataagctgat tgtattgacc cccgaagtcc agtatcaata
     5041 ccagttcctt gtctgccata ttacactctc cattcattag ctgcctcgcg gcacacggct
     5101 cgcagcccgt gcatctttgc atattcctaa tatagataac attagttccc tacagactat
     5161 agttgggagc ttcctttgtt atgcttacat cgtgggggtg gctttctctc aaacctgcag
     5221 cggttatacg catgaactgc gtatttttcc tcagctgctc aatagtatgt gttccacaat
     5281 agcccatacc ggatttcagg cctcctacca attgataaat cgtttctgcc aaaggccctt
     5341 tataaggtac tctgccttcc actccttccg gaacaagctt cctcgcatcc tcctggaaat
     5401 acctgtcctt actgccgcat gccatagcgc ccaaggaacc catacccctg tataccttga
     5461 agcttcttcc ctggtaaatt tccatttctc cggggctttc cgcagtaccg gcaaaaaggc
     5521 tgcccagcat aactgcactt gctcctgcgg caattgcctt tacgatatct ccgctgtatt
     5581 tgactcctcc atccgctatt atggatatgc catatttatc tgcttccgct gcacagtcat
     5641 atatagccgt tatctgaggc actcctacac cggtcaccac tctggtggta cagatcgaac
     5701 cggggcctat acctaccttc acagcatccg caccggcctc gattagctct tttaccgccc
     5761 ctgctgtagc tacattaccg gcaatcagct gcagttcagg gaatcggcct tttatcctcc
     5821 ttacagcttc tataacaccc tttgaatgcc catgggcagt atctattact acaacatcga
     5881 cctttgatga taccagagcc tctgctctct caatcatatc ttcggttacg ccgacagctg
     5941 cgcccacaag aagccttcct ccgggatcct ttgcggaatt tggatattgt atggctttct
     6001 caatatcttt tattgtaatc agccctttaa gatatccttt atcatccaca agagggagct
     6061 tttcgatctt gtgtttcttc aatatctgtt ccgcctgctc taaattggtg cctacaggtg
     6121 cggttataag gttttctttt gtcattacct cggatatttt ttttgaaaac tccgtctcaa
     6181 agcgaagatc cctgtttgta agtatcccta ccagtttgcc tttttcatca gttataggga
     6241 ctccggaaat cctgtaccgt tccatcagct ccatggcatc ggatataacg tgatcggggg
     6301 acaggtgaaa gggatcaact atgactccgt gctccgacct ctttacctta tccacttcca
     6361 tagcctgctt ttctatggac atattcttat ggattattcc gataccgcct tcccttgcta
     6421 ttgcaatagc aagctttgac tccgtaacgg tatccatccc tgccgacatc agcggaatat
     6481 tgagcttgat tttcctggta agcattgtcg aggtgtctgt attctttgga agtacttcag
     6541 attttgccgg aaccagtaga acatcatcaa aggtcagacc ctctttcaca aacttttcca
     6601 ttgggcttaa acacactcct ttttaatttg tagaaaaaag tctgcaatag tgaaattgca
     6661 gacaagaaat ttcactcata gaaacataat taacggttat gttgtagata cttccgagcc
     6721 gtatcctcga aattatatga gtttaaaaaa cgtattttta atagtatatt taatgctgca
     6781 gtttttgtca aattatttct gactttgcag aacccggtta cagagttgct catatgccgc
     6841 tcctcctgtt tacagtgctc ccagtataac ccctgcaagc tgtccgatta taagcccggg
     6901 tatgcctaaa gcaataagcc caagtattcc cccgaggaat ccgcctataa ggccatacat
     6961 aatacatact ataactgcag ttgctttgca atcttacttt tttatttaaa tacatataat
     7021 aaaaatagta tatcctctct tacattgatt ccatcctccg ggggggtttt aaaagttaaa
     7081 ctgaataatg agctgatttt ctcaaaaaag agtgcaggca ggttcccaaa agaggatgag
     7141 gttgaaaaac tgccgatagc aaggctgcag tgatatatga aagggctaca aatctacacg
     7201 ccataccaga tggtgtgctc ccttgcccca gtagtcctcc attatgcaag acggtttgcc
     7261 ccacttgccg gtccaaacct tctgtgcagt cttataaccg ctgtcaaggc aaaactcggt
     7321 atatccctta tttttcaact catcgtacat catcctgagc atcatgcttc ctattccttt
     7381 atattgataa tccggatgaa caaacactgt ccctatttct ataacgtcaa tgtatcttcc
     7441 ctttgtgcat ttcataatca attcatttgg cctgccgtat tcaatagtgc ctatgatcct
     7501 gcctttgtcc cttacgacca gaaaaaacct atcacggcct tcactatcca tatcctgcat
     7561 aagatacttc ttcttgcact caatttcgcc ttccatcagc tccgtcaaat gggaaatacc
     7621 atttttcgca aaggtatccc tgaccacgat ttcgaaaaac tcgtttatag agcatatttc
     7681 ctccggcttt ggcctgccaa tctgataatc attcattaaa atcacccctt aacaaaaagt
     7741 ccgcccaatc aagtttaagt ttatcggtat tttcttgcca gtacaacagg cgtttatagt
     7801 agtaatttat cttttcactg accggtatta aaaagttatc ttttccgtac cttaaaacaa
     7861 ttgacgtcaa ttcatacata ggcagcccca aggcatgtgt ccctacatgc acggttgctc
     7921 cggcatgacc gattgcgtgg cacaaagcgc catattcact gtcattgatt tcttttgccg
     7981 ctgcatgtga attcaagata gccttttttg ccgtgggcat tttgattttg cctcttgccc
     8041 aagcctcaca aagctccaaa catattcttg gcctccgttc atcaggatac tttgcttcaa
     8101 attgctccaa aggcagtttt gcacaatcca gtgcccacat caccagggtt cggtgattct
     8161 gcagttgaat aagttttatc agctcttgca aacatttact gtcgcgggaa aacaatattt
     8221 tatttctttt ctttaatttg atttctacat ccgaaaacat agtgtttgtt ttcaatcata
     8281 atacttcctt tcccatcatc accacgttat cataaacacc gtcaagcttg cgtgatttta
     8341 ttctcttccc ctcgtaaaca aatccgaact tctcataaag ccttatagca ttgatattgc
     8401 tttcaaatac ttcaagttca accttctcaa taccattctt cttcactgca tgttccatag
     8461 ccttttcaat cagcttgctg cctataccct ggtttctata ttcgctcaat aagcctgtgc
     8521 ctagtattcc aacatgagta aaaccttcat ttctttttgg aattatgtca caccatccga
     8581 taactttatt tccatcaata gcataatatt gggcatagtc atttgctaca ttcatactga
     8641 caaaatcttt taccccttgc agcggaaatc ccttgacaat gcctaaatac ttacgttccc
     8701 ttgctacgac gtcaagcgct tgatagaagc tttcagcgta ttcttccgac gcttttatga
     8761 tctcaaccat ttatattcct ccttttaatt actataaatt acctattgag ggtttatgag
     8821 gtgtggttgc atgaggcatt gttaggcgta agtgatttta ctttaaaact atcggtaaca
     8881 tcccaatcat tccagttata tttaatgcac agtgtgttac aatgccaatg taaatatttc
     8941 ttttccacca aaccacataa acaagaggta atagtgctat tattctagta acattctgcc
     9001 agggagtgaa gaagtggtaa agagaaaaca acaatacgtt gattattggc gcccatcctt
     9061 tcatatgctt cattctgggc agtaaataac ctctgaagta gagttcctct actattggcc
     9121 cgattatccc gttaaaaatc aggccaagaa tccacgtaac caataagact gtcttagagt
     9181 attggctaat atgttgataa aagctatcaa ggaaaaacca tttcgggacc caataaaaaa
     9241 aattgttaac gaataatgtg tcaatcctgg tagacaggca tctgaaaaca aattcactcc
     9301 actttatcag tatgacgaca agaataataa attgaatgac aggcaccttt tctctaaata
     9361 gcaccacatt attaagggat attcttttgt tcacctttct accttgataa atcaaaaatc
     9421 ccaattcaaa gggaaccaaa attactgcaa ttgcaacaaa aataactaaa attgaaggta
     9481 ttgaaaacgg aaccaagtac cagctaacca gtaagtagaa tgctaaaatc aaaatgcccg
     9541 gcaatagcga caatacaata atttccagcc ttctctcttt ttcagttggt cttgtatggt
     9601 taaactcgta agtcaaattc cttgcataat ctctttctct tactagctct tcgtcaacag
     9661 cactataata atctcccgat gacatttttt ctttttcacc ttttgtcata ttcttctcct
     9721 tcaaataaat cactgcagtt gaaagaatca tttttcatag tcttccgcaa aatatcgctt
     9781 gcctttcttt actactttat ggttttcaat ttcaagcatc atcttttgtt gatcaatacc
     9841 gcccgggtac ttttcattac ttcatgatat tcgataggta cagctataac catattgccg
     9901 cccatgccgc agcgtgaggg cattgtttga tgcagtaagt acgctatact atactccatt
     9961 agttcttttg ccttgctgca acagcaaatt ctaatgactc tgcatcgtaa ggcagtccgc
    10021 aaacatccga ataaaactcc aaaacctcaa ggccattgtc ctcaaattcc cttctgagtg
    10081 tttcaacact gtaatactgc agccaattat atataactcg cgtttgagcc ttttcaataa
    10141 ttgtgtattt atcaagtatt attttctcat cgcagtactt aaacgtatta acaaagcaat
    10201 aataatcctc cggagaccaa aaattattta gatggttgaa ttcataagcg gcgatttctt
    10261 ctctccggtt atatgatttt aatgagtaga catcaagtaa aacatttcct gaatgtttca
    10321 gaagctcttt aaatttgttt aacataatac ccctttgttc gggactcaac gcacaataat
    10381 cacacataat cattgtaatt agatcaaacc tgtcttgtgt ttcatactca agataatttt
    10441 tgtgaacata attaactttc aagtttttgc ttgaagctat ctgtgcggca tactcaattg
    10501 atctttttga aaagtctact ccggtaacgt taatacccct ttctgcaaac ctattagtgt
    10561 aaagtcccgg gccgcatcca aaatctaaaa catttgattt gttattgaga ttaaactttt
    10621 cgattatcca ggatacagat cggttaataa aacgagcatt tcgagaggaa acgtctatct
    10681 cttcattaag atgataatcc agcatctttt ttgatgtatg ttcatcggtc catagttctg
    10741 cggcagtata atactgaaat ggttttggcc tttcattgat tgcttttaaa ttattgaaca
    10801 taaagatatc tccctaaaaa ttctttcggt gtttattgca tataattacg aactaataag
    10861 cgctatccag cccttgtgcc attaggtaca gctttttccg gctgagccaa tacaggaaca
    10921 ataccatctg catatccaat atcaaataac atcccttcgg aaagttctcc cgccattttc
    10981 ttaggagcca ggttaacaac aaataacgcc tgctgtcctt ctatctcttt tggattctct
    11041 ctttcacctt tcatcccgac taatatagtc ctcaaaaagt caccaaaatc aactgaaagt
    11101 ttaaccagct tatttgaatc ttttatgtcc tcaactgata ttatggttcc tacccgaata
    11161 tcaattttat caagtatatc tattgtaata ttaggcttta ctgaagcact cattgcactc
    11221 tctccttcgt aaattattta ggattgtatg tatatctcaa ttctttcttt accactgccc
    11281 aaattgtttc tttttaagga tatagaacta atttctcctg ttttttttaa atgcgtccct
    11341 ccgcagggaa ctttggcaaa cccttctatg ggttttccca aaatattttc ttaacactcc
    11401 tcatcatata gcctctcttt cacatgtact ttatatatta caattctaaa agtatgcagt
    11461 atagctgctg ttactctata tattgcgttt tacatatagg acaagcattt ctgtgtacgc
    11521 taacaatatt gccgcattca atgcaagccc atcgagcttc ctcattcttc aggaattctt
    11581 tcataccgtt ttgcttaata ttctcaagat tttcaatcat gctcatgtga tattttgttc
    11641 ggtatcgttt atccagttgc ttcaatcttc tgcatggaaa tttatcacat tcatagcata
    11701 agccagaact gctattctgt atggtaacac aattcttgat aatacaatgc aggcaatacg
    11761 ctggttttgt acaatcatca ctattgcatc cgatgcactt gtttttttta cgttgaaaag
    11821 ccatgcaaag cctgcaattc ataccgcacg gggcaataag ttctgatctc atattatcca
    11881 ttcaattccc ctcatacttt ttattgatat actggcggat cagcctttcc ggtgaagggg
    11941 tgtaaagtgc ggcaacgatg ccgcggctct atatataacg tctaacctta ttcatatatc
    12001 tcatatattc ttctccaaat ttcttgatgc accatctttc ttccgatagt ataatccaat
    12061 gggaagatat ttgaaaaact atcaataatg taaataacgc ccatgatttt gatataagta
    12121 cacatcctaa caaacaaatg aaatatgcaa tgtacatagg attgcgggat attcggtata
    12181 agccttttac attcattcca tgaaaatgct atgtttctta gagttctgcg aagatttcat
    12241 gaatattccc ttctaggtct gcaaataatg cagttcgttg attccacggc atattttgag
    12301 gttgatgtat aggagttgct cccttcgaaa tcagccattc aaaggattta tcgacatctt
    12361 caggatcttc acaaggaaag gcaagctcaa atgcctgtcc accagttttt ttctcatatt
    12421 cactactgta agcatacatg acttccctta aacaaatagc aaaccgaacc ccctcattct
    12481 caaattcaac ataatttccg aggtcattac taatcttaaa tccaagcacc tgattataga
    12541 aatttttcat tttgtttata tcatctgccc aaattgtaat aaggttaatt tgtgctttca
    12601 tagcaatttc cacccttcag atttattaag gtctctttta ttatcttatg gatacgcatt
    12661 ttatgcttca aggtgctctt cggataacct tataatatta acacctctgt acttgtcagc
    12721 aatttttctg gcgagactct caatcccaaa tagttcagta aaggtatggc tgccaattat
    12781 taaattcatt cctacaaatt ttgcatactc aatagtgtaa aggacttttt cgccagttat
    12841 atatacatca caattttttt caaatgcctc tcttacatct gcagttgaac ccccgccccc
    12901 acatactaaa ccaactcttt ttatacagcc atctttgaat ttccacgcct ttgttggttc
    12961 tccaagaatc atctccaatt ttctcacaag ctcttcaaat tctacctctt cgtcaaactc
    13021 agctattctt ccgcaataga atccgtcttc attatgagat ttcttgataa tacttaaccc
    13081 cagcttttca attaaagaat tatttgtccc aaaattacaa tcatctaatg gcagatgagt
    13141 aaaataatgt gatatgttgt attccgataa tttttcccgg catgcatctt taaggccaaa
    13201 tataaaatcc catgcatcat gatgagttat aatcaggtca acgttatttt tcttgccttc
    13261 ttcaattgta tcaagagtta aatttgtaca atatcctatt cttctaatct cttccgtacc
    13321 ctctactgta gttccgcttt gttcatggaa atgttcattc tcactgcttt caaacaagct
    13381 tataatgaat ctctctatct caactaatct catattctct cctcctttat agttaaaatt
    13441 ttatctgcat gtcatctaac ggttctgcac ttacgacatc cctgatctga gcaataattc
    13501 catattgttt ccgatacccg gggtcttgcc tgttccgctt gtacagatat tgtctccctt
    13561 tggcttaaat cactaattat aacgctgcat catatatgcg agaactgtct ccccacggat
    13621 gataacctgt accgatatac ttaaatccat acttctcata atatccaata tggtctgtac
    13681 agaggtataa gtgagaaaag cctcctgctt ttgcgtctat ctttgcctgc tctattagaa
    13741 gggacccata agcatttcct ctgtattctt cctcaatgaa tacagcacaa acccaagggt
    13801 ataaatccat acggcttata aaatcgtttg ttatgagacc tgcgcagcca attattctat
    13861 cattatcaat caacagatac cattgaggca atggactcag ggtagtaata caatttgtta
    13921 tgcagtcatc atagaccttc atactatttg gggatgccca tttactctgt aagtatttaa
    13981 ttgccctatc tttatactca ggagattttt ttatagatat gacttccatt tccttcaccc
    14041 ctttgtgata tgcaaacatt tttcaacctc cacctttgca tcacccttct tcatgcgcct
    14101 acttcctgaa cccgcttcgt gactttgcta tctgcaccct tgcgctatca gaaatctttc
    14161 tttaaataaa ccatatcgac caattgaatt ccatcctcat aaatagggtg gtcatagtta
    14221 tttgtaaaga aattttcaac tttatgagat acttcaaatc cacaatgttc ataaaatgaa
    14281 attatccacg ggatgtctcc tgtaccgact aatagtgttt tacacttatc cctgcagtaa
    14341 tcaaatatat aacttattag gtattttcca taaccttttc cgcgatattt ttcatatgta
    14401 gctaagtttt tcagctcaca ggtattttca tcttcctgcg ttactacaca aatgcttttt
    14461 aaatctccat catatagagc aaatacttct ccccgttcta aatatttatc aatcatatct
    14521 tcttgttcat ctgctaatag tagtaaatct aaaaaaagct ttttatttcc tttaataggc
    14581 ttgacttcca ctcttaaccc ccccataaga taaatatcct ctgtactacc ttttgacttt
    14641 agctatcaac tgaataatat taaaatactt tccaccctca gcctccatca ttttgatgtc
    14701 ctcagcagca atgggatgat cactggccag ccattcttcc caagcctgtc tgcaacagcc
    14761 catttcacga atgtccatca cttcaatgcc ctttgcactc ttccataaat ctttccacca
    14821 atcgagagag tgcaaggttc tttccatgtc gctatcccaa aacggctgca tttcatctgg
    14881 gacattttcc ccgaattcgt attttaagcc gggaatagcc acggcgatat gcccttcctt
    14941 tttcacaaaa ggaatgagcg atggaagcat ttccgccgta tcaccaaaat aatggtaagc
    15001 gtcaacggtg aacaacaagt cgaaatatcc gtttgcgaaa ggtaagcctt tggttgcgtc
    15061 tgaaaaaatt ggaacagcct tttcatcaat tccaacggac tcaaaacgct catagttttc
    15121 agttggtgaa atccaaaggt cggcggcaaa aacagacaca ccgtattttt ttactaacaa
    15181 aagcgtggaa aggccacacc cgcaaccgag gtcaagaatt cgcatattct cattaatgtt
    15241 caggtgtgac gccaattcct ctgctaaccg catggcgttt ggccccatca tattggcttt
    15301 aaggaactca acatttttat tatttgacat aaactggttt gtaaatgtat acatggcatt
    15361 catcctttac tcttgcttac ttttaagaat agactcatat ctctttgcaa tatctctatt
    15421 acgagcggaa agccatattt caaaggattt ctttgcgtgt aagtaaacaa tggagaattt
    15481 taatccttta tctttaagtg tttttgtgct tactgaaaaa tagctcatgt ccgtatagcc
    15541 ttgatataaa ctgcctatgt cgtaatgcgg atattttttt taatgtctgc tctcaatttg
    15601 ccgataaact ctaatatccc tttgtacgca gcttgtattt cgccctgctg cagatggtat
    15661 gtgtacttat ctattagtcg gttcatagtc tttatcactt cacttcctaa taaatttcca
    15721 atacaaaata ttaaaccagt aaatttcaga gggccccaca gggactttcc ctatatctta
    15781 ctataccgtt tccggtaccc cggacttgtc atgtgccgct taaataaata ttgtctatat
    15841 taggttcaaa tctctgacaa gctctgcatg ggggcaggaa tgatgtgtcc gattatcatt
    15901 gagataattg ttataatcct agcctgttct ctttaatttc ttcctcctta aaatttaaag
    15961 aaaatttata atgaaagtta tgcatgcctt gtttttcata aaagttatgt gctcttgatc
    16021 ttaattgatt acaacaaacc tcaatttgaa gacactcatt gtctttcgca atttcagatg
    16081 ctttctcaaa aagtttcttt cctacccctt tagaccggta tttatccatt accgctaatt
    16141 ctaaaatttc tgcaattttt gctgtatgat gaagttgata ctctatccga agatttaaac
    16201 aaccaatgac tgtcccgcta tgttccgcca caaggcagaa atacctatca tcttcaagat
    16261 attttttaaa gacctcttga aatctttcat aatccaattt tgaattttcc atatcacaaa
    16321 tcaaatcata tattacatct atatcagaca gttttgctat tctaacagtc atttttctca
    16381 tctccatttc tcatatagta tacatataaa tcaactctgc ttcacatatt ttggcagtat
    16441 gacggctatc gctattgttt cgtgaaagta ataattatat atgcattatt ttattgtcaa
    16501 gcaaatgggt tcttataaag gtaaaataat ataccaattg tcaaaatcaa cccgccaaga
    16561 atccatgttg gtattcccca cacatcattc ttttctcttc ttacataagt atcatacaat
    16621 gaaaaaaata taattagcaa acctgcaaca atctcggtta cctcaccact gtttctagcc
    16681 ataaatgtcg tgctttgctt aaaagcaaac atcaaaaccc atacccctaa tatacaattt
    16741 ctcaatatag caggccctct gccagaagat ttaaatggag taattcccat actcttacct
    16801 cccattgcga ttgcattaaa tctaattaat tatgacttct ctctattagc ttgcagccaa
    16861 taacttcagc agcaacactt taaattctac atacttgttg cgtttctaat atggcgtccg
    16921 gtgaaacaag tattggcaga tctgctacgt cccttatatc agggtactta tctatttcga
    16981 taacttgagg tgtataaaca aaggatttat atcctaaaat aattctatgt tttctacttt
    17041 ttgaatttta gcatggtatg gcgtataacg gttccgtgct accgtagcca ccgagcctta
    17101 aggcgtcagc cgggcgcgat gcgcttaggc ccggggatta gtatcgtagc gaagcgaagt
    17161 cggcaacaca ttgttaggca agtctgatac tctagtaagt aaattattaa tacttcataa
    17221 tgtcttctaa attttctaat atgtaggcct tgccaatatc gttaattacg accatctcat
    17281 attcattgcc attatagttt cttttttctg attcaaaacc atagtcaacc gatttattat
    17341 taataatcgt ccttacctta ccttgctcat caatattttc actcaacact cccattttct
    17401 ttaactgttt gtttaattct actcccgata atttcttgct tttatttaaa tcaatcacat
    17461 tattaataca ttttgcaaac tcatttaccc caatcttatt ttcaggaaac ttaaccaaac
    17521 ttttttcttc ctttgtgata tgaagctctg ttggcttagg caagttttga tttgtttgtc
    17581 cagctattac ccggtttagt aattcggata tgtaaaaaaa acatcttatg attctaggat
    17641 cgtttaaaaa actttcttca ttaataggcg ttccatcgac aggatttatt ccatttgcaa
    17701 tcttaatcaa aacttcctta gccttaataa ctttttctct ttcattaatc aaaatgtacc
    17761 acccccttat atacacaaat aagatttcgt ctaacgtttc cgcactcacg acaccgctga
    17821 accgaacacg cagagccttg ctcttttttc tataccgttc cggtacccga aaccttaaat
    17881 gtgttgctta tttagtattg tctctcttga gtttaaatca tacaaactcc gcatataatt
    17941 tgaacattat aagaagaatg cgaattaact caactttaca ttatgctcat gccaataagt
    18001 tactctaata taaacaattc ttccactgtc gtatccaata acctggccat tttcatagcc
    18061 aattccagtg taggattgta tttatcgttt tctatagcaa ctattgtttg ccttgaaaca
    18121 tttaatagca tagctatatc ttcttgtcgt aaacctttgt tttttcttaa ttgtttgatt
    18181 ttattcttca tttttgaatc cccgaaacga ttaataaagc gccaatcgaa acaataatcg
    18241 cacttataat tacacctact atgataattt tcaaaagtgg attatcgtct ttatactcat
    18301 catcatcttt tacaactctt ttttgtaaca cgagttgtga gaaaacttga accaaactag
    18361 tggttactaa taaaaaactt ggtattgagt tgatagatgt gtgttgtgaa tacgctttaa
    18421 aagattcata gaaagaccac attgctaagg ccaataaaac ataaatcaaa gcattttgtt
    18481 gtgctttaag tgcaatgttc ttttccattt catccatttt acgaaaccct aattttgaga
    18541 aaagtgattt catagtacac ctccaaatgt aaaaagttct ttacattcat tgtatcataa
    18601 aggttattaa tgtcaagaac cttttacatt aataatcttc atttcgcttt tttttaccat
    18661 catacaatat agcaatcaga cttcttatgt aagccaattt gcaccatctg tcgcataacg
    18721 gtcccgcact cacgacacat ccgaactggt cttacagagc cttacccctt ggcggaacga
    18781 ccctcccggt gaagggatgt ggtaatgccc aaggcacgaa tactacttgc acctgcccat
    18841 cgcgccgaag cgtgaaggca ttgttatata aagcaccacg cctccagagt caatagcgac
    18901 acggatgccg cggtccaata aagctcttgc ctgatcttca atacataact atttgaaaaa
    18961 cagcgctatt attgccccaa ataatcttgc aatttttact ttgaaccaat ttgttctaat
    19021 agtagtataa aatacacact ttggttcaag ccacccttta ttgttccaat atgtataatc
    19081 aatactattc tgtgagtctg tattcttttg ccagagcttt tgctgtactt taaatataat
    19141 aagcgaataa agcgtaggca taaaggcttt ctgattcttt atatcatcaa ttagctttct
    19201 tgctacttca ttaactttac tatgattgtg ctcgtatatt tcttctcttt tcatcaaagc
    19261 gcctgtacga aatttatact gtccgccgag atatgctccc catgacataa ttgcaaaact
    19321 aattgtcttt aacgcatgat tggttgatcc tgctcccgat gtcgttatag tcacagctac
    19381 cttacctgcc aatgctggcc tatggcaata aaatgccatc ctgtcgatcc agttcttcat
    19441 aataccatta acatcctcaa cataaaccgg gcttgcaagt ataacaccat cagcttcaac
    19501 caccttctct ctgatggtta ataagtcatc cttcaacgga caaaattgtt cccctttatc
    19561 aaagcagatc ctgcacccca tacacatttt aagattagcc tgtccaagat atattctttc
    19621 aatttctatt tcttcatgtt ctaattctga cactgatttt agttcttctt ctaatatact
    19681 aactagattt tctgtatttc cattcttccg agaactactg attattgaga ttattttcat
    19741 aaccttacct cttgttatta gtattttcat tggattataa attcttaatc caaaggcttc
    19801 ctttcaaaca cccgcacttg ggcggcactt caaagcaaga tatttcacaa aacgggttcg
    19861 cactcacgac gtccctgaac cggaccggca gtgaccttcc ccctggcgga gatttgttcc
    19921 cagtgaaggg gtgtggcagc gcccaaggca cgctacttaa tactccagcc caacctgccg
    19981 cagcgtgagt gcattgttag atggtggatg atgccccgca acgcctcgct taataatctt
    20041 tatataccgc tttcggtacc cgggatctga catgtgtcgc ctgtataaat attacctctc
    20101 ttgggtttaa atctctcaca aactctgcat agaagtttac acataatgtg aacagtttga
    20161 ttcatcaata attcttatcg cttttatatc caacaattaa gtaaagaaca cctaccaaaa
    20221 gtggtacaag catatgagac actaattctc cttgcatgaa caatcccata agtagtccta
    20281 gccctaccga aatgatcaca agatagccgc cagttttttc gaacttcaaa ccaagcgcaa
    20341 gtcctataaa cataatcggg aaaatcatta gccacatgtt gtcccataaa gtgtaatcag
    20401 ggttaataaa gggcagtgga ttgccatatc caaaatagaa cggcaaaccg aatgcaatta
    20461 taaccatagc gctaattcgt gcagcccatt ttatcttatt taaactctta ttattttgac
    20521 tcattcactc gcctcctttt tatataatat attcagatta tcgcactgca aaactaaatc
    20581 ttttcacttc gcaaattact aataatacga aatataagaa tagtacttct catataagtc
    20641 ataatctgca ttatctgtca gctaacggtt ccgcactcac aacatcactg aaccggaccc
    20701 acagagcctc tccccctggc agagcgaccc tcccggtgaa gaggtgtggt tgctacttaa
    20761 gtcagactac agtattgcat atctttgcaa cccaaggcac gcattctcct tgtccttgcc
    20821 caccgtgccg cagcgtgagc gcattgttag atgatgtatg gcgggctcca gcggcaaact
    20881 gaataaaata taaaaatata aatttttctc atattttatc attattatga tataataata
    20941 ttattataat gataaaaagt tagtgaggtg agttataatg cctattatta gaccaatttc
    21001 tgatctacgc aataacttta atgatatatc cgaaatatgc catactgaat ccgaacctgt
    21061 atttataaca aagaatggtc aaggtaacct tgttgtaatg agtatggctc tttatgaaaa
    21121 acagcaggca cttattgaat tataccaaaa gttatctgtc gcagaagctg agagcaaata
    21181 ctccgacaat agaatctctc ataaaaatct tatgaaagaa ttaagggaga ctatcaatgg
    21241 aaaagaagta taaaattgag tacctgccaa gttctgcaaa ggacttagcg gaaatagtcg
    21301 attatataaa aattgatagc cctcaatctg cattaaattt cctagataaa attgatgaat
    21361 caatctctcg tcttgaacaa ttccctttta taggtgtaat cccaaaagat attaggcttc
    21421 aaagcttagg ctatagaata ctaataattg acaatcactt gatcttctat gtagtgttcg
    21481 acgatatagt tgaaataaga cgtatcatat ccggcaaaag aaagtatgac tttctatttt
    21541 agcatctcac taggtattta gcagagataa tactactact ccatgaaatc cgccatatgt
    21601 cacataacgg gttcgcactc acgacatccc tgaaccggac ccacagagcc ctgccccctg
    21661 gcggtggtga cacccggtga agaggtgtgg ttgctactta aatctaacta cagtttagca
    21721 tatcctctgc aacccaaggc acgtctgccc catgacactg cccgacgtgc cgcagcgtga
    21781 gggcattgtt cgtatactag gttcataacc agcttacctg ggtatgaact ttttccatta
    21841 tcttgtatgc ttagattgaa taatctgggt gaaaaacgtg cgcgttgttg ggactctgat
    21901 cccgtcgcat aaactgtttt agccttgctg acgctcatac ctcgattgag ctggttgtgc
    21961 ttttagcacg catcaccgta cgaacctgtg gagggtcgcc agatgaggaa acccagaata
    22021 aaaggaggta ttctatgaat aatcctgccc tttgtatcga tgtatccaaa tcatcaagct
    22081 ttgctaccgg ctttttatcc tacaataaac cttactcaaa accctttgat ttctcccata
    22141 ctcccgatgg aatgaaatcg gcacttcatt gtcttaagga gctgcaagac aacagcaata
    22201 gcaaacctga tgttgtcctt gaggcaacag gcaacttcta caaacctgtc actcagtatt
    22261 ttattgatct tggttacagt gtatatgtgc ttaatcctct ccagacaagt atggagaagc
    22321 gtaagtcaat ccgtaagatc aagactgacc ccgttgatac ctaccgtatt gctcaagtct
    22381 attacactaa cgatctgaaa ccttttaagg aacaatctca tgactcgcag gaactacgaa
    22441 gtctttgcag acaatgggac ggcttcaata ccttgaatac tgaaactcaa ttaaggttca
    22501 gaagtatatt ggctcttgtg ttcccaaagt atgattctgt atttgatcat ttatgcaatc
    22561 ctacttctct aagggttatc gcttattttc cttcacctaa ggctatcctc tcagctaaac
    22621 gtgaagagct ctttaacctt attaaactat gtagacactc caatgaatgg tgcaaccaca
    22681 aggtggacat gcttattgct gctgcaagag aaagcttatc ctttgatggg gcccaacagt
    22741 caaatatccg ggttctgcaa agctatgtga agtccctgct gacccacaag gaaatcctgg
    22801 ctgatatacg agctcagatc aatgaatgga ccaagctctc tcacaacttc caactcctac
    22861 tatccatccc cggcgtggga gaagtaacag cagccaccat tcttggagag attggtgata
    22921 ttagtaactt tgatactgct aaacaattag ttgcatatgc tgggctggat ccttccgttt
    22981 atcagtctgg cagcttcaga gcgaagaaca ccaagatatc caaaaggggt actccctact
    23041 taaggaaagc actttatcag gctgcatcag cggctgtcag aaagcggcct aaagggccat
    23101 gcaactctgt gctatatgaa tattatacca ataaagtaaa tgctggaaag cctcctaaag
    23161 ttgctttggt tgctacctgc aacaagcttc taaggattat tttcgggata ttaagtagta
    23221 aaactgagtt taaagctgag tagcaaagta ttggtattgc ttatgtagtg cctatgtatc
    23281 cataggttag ctttgctatt gcctatttcc cttagataaa ttattttgct aaaccacttg
    23341 actgtgatta gctggtttat gcgccgtcca cgggctttag cttcagcggc tgacacggac
    23401 gtcagccacc taaatacagt atatcatacc aataagtacc atgcggtgtt tttgcttctc
    23461 tccagtctct caatttcata tcgaccgaag ctccctgccc cacccaaggc acggatgcct
    23521 ggagataata agaaaaagga tagcgtatgt ggcgatatgt atggtacttc tcttatactc
    23581 tataacttcc taatacggca atatattagt ctcgaataaa tgaatccgtg agatggcgta
    23641 taacgggttc gcactcacga caccactgag ccggacctca gagcccttct ccctggcgga
    23701 gcttcgctcc cggtgaagag gtgtggttgc tgcgtaaatc aaaccaaagt ttagcaaatc
    23761 ttctgcaacc caaggcacgt ctactccctg cctttactgc cgtgccgcag cgtgagggca
    23821 ttgttagctg atggctcatg tccacagagc cctaccctaa ttatcattcg ctgtaactgt
    23881 ttagcattag tattatcgcg atttatcatt actaccatta aacaagattg cagtatttcg
    23941 attgtcatcc aaggcccagc atttgctttg ttatttcaat agccaaactc tttactactt
    24001 caagcgcaat cccggacaaa tctttagttc tactcttaat ttcataccac actttcgggt
    24061 ctcgaataga gtctaaatag tcgtgcccgt tccaagtaat ttccttgatt atcatcgctc
    24121 ttggggctac ttctttgttg gcaacctcga tatattttgc ttccacaagc ttctttgctg
    24181 aataaaaaat gtcttcttgt gagaacccag atagatcgtc ttcgtctata tgtcctaatt
    24241 ttaagccttc aggtaatcca ttagccttaa cttccaagtt ttcttctaag taaattaata
    24301 atgctcgtat acaatcgtga ttcagtttca ttttactcac tccttgttta ttgatcaaat
    24361 ctccatccaa tcatgtacat gagctgtcag ctaactaccg cattcctgtc atatgtcgtg
    24421 aatgatgcca atatgttatg ataggcgacg agtgtcgtga attaccccat atgtaggtat
    24481 tcagttctat tcccttctac tcattagttt atccagcgga ctggcaatat tctttattcc
    24541 cttctctgtc acatgtgtat atatctctgt tgtcttgctg ctttgatgtc ctagaagttc
    24601 ctgaatatat ctcaagtcta tccctccctc caaaagatga gtcgcaaaag aatgtcgcaa
    24661 actatgtaca gataccttct tttgtattcc tgacttttcc ttcgcgtttt caaatacacg
    24721 ctggacggtt ctttcagtaa tatgcctgcc aacctcttcc ccttggaaca gccattcaac
    24781 cggcctgaat ttctgcacat attcgcgcag tacaccgaga gcaacttcag cgatcactgt
    24841 atatctgtct ttcctcccct taccctgaac tacgtgaata agcatcctgt cgctgtcaat
    24901 atctttcagt ttaagccgta caacctcacc cacccgcagc cctgatgaat atataagaaa
    24961 tagtattgcc ttatgcttga aattggttac attatctaat atatgcctca cttcattttg
    25021 gctaaggact tccggcagct ttttctcttt tttgggtctg actatgtcta gcgttaaatc
    25081 atcttccata agcacttttc caaacaggaa cttaattgag cttacagctt ggtttacaaa
    25141 tgaatgtgca ttaccttttt gttccaacaa atataacaga taacaattaa tatcttcctt
    25201 cactaattgg ttagcattct tgccgcaata cccaagaaac aatcttgcat gactcatata
    25261 tgactttatg gttttttgac tatatcccct tagttttaat ccttcttcca tcttttttag
    25321 aaactggtca ttggagctgc ctttcacaat aaccagttct tctttcgtaa acagctcctg
    25381 taattgatta atggactcct cacaatccgg taccaaccat ttcttattgt ctttatccca
    25441 tctgcgcccg ggtatccccc ttactttagc aatatattcc tcattatatg gaaacacaag
    25501 ctccagcact cccggttttc cctttctcac acaaattgcc aaaagaatcc cctcttttgc
    25561 atcagattcg ctgccaacat ttacctatta tattctatta tacgatatat gctttctaaa
    25621 tcaaatacac tatcatataa aatccgacac tatttatacg ccaaattttg atattcttca
    25681 atacatcccc attgtatagg aaagtaatgc tttcgcgatc aaaaaaaaag gacatttcgt
    25741 ttgtcagcat gaaaaacaaa tgtcctctct atttaaataa actgttctgg agacagcata
    25801 aggagtttga tcctctccct gcactctact tcttcctttt gaacgcttca atctcttctt
    25861 ctacttcttc tgcaccttca gcatagaact tttcttcatc aggagccagc tcgtagagca
    25921 gtctgagaaa ttctcccgcg tgaacccgtt cttcatcggc aatatccttt aatacttcaa
    25981 ttgccagcat gttgtctgtt gattctgcca gctgcatata cagctgtatt gcctcatatt
    26041 ctgccgcaac catgaatctg attgccctga ttaattcctg gtcagtcagc ttacggtcct
    26101 tggcaagtcc ggaaaatgag ttaccaaaat caggcatatt attctcctcc tttatttttt
    26161 ggtcttatac ccttataacg aggcgaaaag atgtcctcca acaggcggtg agcatatctc
    26221 cgtctcaagg acagcttaaa cgtccccctg tacctctatt tcgtcgccgg gttttattat
    26281 tccgcccttc agaacctttg taaatattcc ttctgttggc attatgcagc ttcctatctt
    26341 ctggaatatt tcacacttca catggcattc cttgcctatc tgggttacct cctgctccgt
    26401 ttcgcctatt tttagctttg taccgacagg gagcttccag agctcaaggc ctgctgtggt
    26461 caggttttct gcaaatttgc ccgtagaaag cccctcgatg cctaaggctt tcatcttgtt
    26521 tatgctctct atacctaata agctcacctg cctgtgccag tttcccgcat gggcatcccc
    26581 tgcaaggccg tggtcaacct tgaattctcc ctgttctatg ggctttttga atacaccttt
    26641 cttttcgctt atatttactg ctaatacctt tcccttcata catatccccc ttttctttat
    26701 ccgcctattg tgtacatgtc acgatcaata ggcttgttat aaagatcatt cagtgtgtgg
    26761 tgcccgggtt tggcttgtac agcttcttca actgccttct gcaatgccag gtatctgtca
    26821 tcggaatttc ttccccgcag tattcccttt acatctatct ccaggttgga atgcaaacag
    26881 ggcttcagtt ttccgtctgc agtaagcctc agcctgttgc agttgtcaca gaatttgtgg
    26941 cttatgggat taataaaccc tactcttccc tttgcgccgg gcagcttgta gtacatggcc
    27001 ggactgcttt tatcggcggc ttccgccaat tccagctcct tatatctttc cagcaccact
    27061 aaattggaaa tatagtgctt cgatgcaaag ccggcggcat gccctaaagg cataagctca
    27121 ataaatcgga catcgatgtc ataattttgt gtaagcctta tgaaatcacc gatttcatcc
    27181 tcattaaagc cttttgtaag cactgtgttg attttaatgg gtttcagccc aacctccaat
    27241 gcctcgtgga atccctccag gacatctttg agcttccctc ccctggtcaa atattcatac
    27301 ttcttatcat ccatggtatc caggcttatg tttatccttt tgagtcctgc cttttttaat
    27361 tcacgggcat attttttgag aaggcttcca ttggtggtaa gagccaagtc ctttatactt
    27421 tcaagtgctg ttattctctc aataagcccc gtaagcccat ttcttacaag aggctctcct
    27481 ccggtaattc ttatcttatt tgtacccagg tcagcacata ccctcactac ttcaaatatt
    27541 tcctccaagg tcaatatatc caaatgctcc ttcttctcta tgccctcctc aggcatgcag
    27601 tagcggcatc tgagattgca cctgtctgtt acggatattc gcagataatt tatatttctc
    27661 cctaattcat ccttcatttg tgacttccat cctttacaac agcacacgtc aaattatgat
    27721 atgtgaatta tttctgtaaa aaagaataac aatccggaaa aggattgtta ttaataatat
    27781 atcaaaaaat atatcataaa atattttcct ttcctaatac gggaggctcg gggatttctc
    27841 cctgaaccct tatgggattt gcccatcggt cgtggcttca tggttgcctt tagaagcgat
    27901 gactcggaaa acttatatat ttatgtccaa ttatatacta taataattgc ttttacaatt
    27961 cttatgcctt agcatccttt gtcggagctg ctgctgtcgg agctttcatg tatgctacat
    28021 ggtagaataa cggaacaata agcgctccac caatgagatt gcctatagtt acaggtatca
    28081 gattattaat ccaaatctgt ccccaggtaa ctggtgctcc caagaacatt ccaagaggaa
    28141 taaagtacat gtttgcaaca ctatgctcat atccgcagag tacaaagagc ataatcggga
    28201 accagcaagc gaatatcttc gatattacgt cttttgctcc tgtggccatc catactgcaa
    28261 gaacaaccat tatattgcag agtattcctc ttaatactgc agcgccaaaa ggtatggcga
    28321 ccttgttaga tgcgattttt atagcagatt ctgcaatagg agttcctgcc gccatcaatc
    28381 ccgtatttgc aataatagcc gcaagtatga ccgaaccaat aaagtttgct gtatagacaa
    28441 agaaccagtt cctaagaata tccgatacct tgtaacggca gttcagacat cctaaagtca
    28501 tcaggttatt acctgtaaac agttctgcac cacagataac caccagcata agtcctacag
    28561 ggaatgctcc ggcaaatgca aacttctgca aacctgtatc aacctttgcc agtgtctgtc
    28621 cgatggttat tgccccctgt gctccaaagc ctatgaacac acctgcaaag atacccagca
    28681 ccatcatctg cgcaattgag agctttgctt tcgcggcacc ggcatcgcag gttgccattg
    28741 caatttcctt tggagtcaac atccttttct ccattctaaa agaccctcca ttctaaactt
    28801 tgttaaatgg tatatataag ttaagtactt ttttaacaat ttaagtttag catcttttta
    28861 acaatatgtc tataattcta gagagttcta aaaattcaga tttcttgact cccgtataat
    28921 tactcgatta ccgatatttc tcctgaatta ggatccatta gtaatccgtg tatttttaca
    28981 tccttcggta tatatggaga atttttcagt ttgagaaccg catcctttac gttttctgct
    29041 tcatcctgca gcaggccgaa ccattccttt atatctatgg cttctattgc ctcctcaggt
    29101 atgcctcttt ccctcatttt tgctcttata tcctctggtt tctgctttct cattccgcag
    29161 tcactatggc ctaccacgta tacttctttt aatcccatca gtactgttcc tacagctacg
    29221 cttctgataa cgtcactgca gttgtttctt ataatgttgc ctgcattctt aagtattttt
    29281 atatcccctc tgccaaaccc catggcaggt tccaatagtt ccaccagcct tgtatccatg
    29341 caggtgaata ccattatatc cttatgggca tggccgctta tgggtttgtc ttctcctgca
    29401 gcctttcttt cctgcacaaa tttcctgtta tgctccagta tcttttctaa cataatgacc
    29461 tcctgaatat ttatttaatc atttctgaag aactgccgtg tttcaattca tattttacat
    29521 tattgttaaa aaatacacaa ataaaaaacg cgtctcacgg cgcatttttt tcgctagggc
    29581 tttaaataag tacccttcgt ttttgcttcg catcctgagc accttccttt atcgacaggg
    29641 ggcttctccc ccttgcatcc cccatcgtat aggaaattat aatttcctat acgataaaaa
    29701 aacgcctcgt accgcacttc aatcaaaata tattatttct cctcttatat ccctgcctat
    29761 atgcaaaagt cccaaagaat acctggactc aaaccttttt gaggtcggtg aacccgggtt
    29821 gaaaaataat actccgtcaa ttacttcatt atgcggtttg tgactgtgtc catatacgac
    29881 acaatccaca ttgttctttg caaacattga ataagcgccc gcatatgcgt ttaccctgct
    29941 tgttccgtgg gtcagcccta ttctttttcc gttagcctca attattcttt ttgtgctgta
    30001 tttatacagc atttcataac tgtcgttgtt tcccgccact gcataaacag gggcaatagt
    30061 ctcaagttca attattacct catcaataag tatatccccc gcgtggaata tcgcatccac
    30121 gccgtcaaac acactgtata ttatgttggg aaggagcttc gcgctgcttc ttatatgtgt
    30181 atcagaaatt actcctatct tcacctttaa tatcctccag ttcatttcct gcttcagatt
    30241 ccttatcctg gtctatgcag ctttttatcg ccaaatccag gtatagtatg gaaataagcc
    30301 agtatattaa cccgaatccg ggtatagggt atccgtatag gttaacggct aattgaacaa
    30361 gcattggaag tgtcagcgca tatatgctga tattcagcag attattgtat ctcagcctcc
    30421 ctttcagcaa agcattgact gcaatcccca ataatgccag tatgactgca ttaagcagct
    30481 tccatcccaa tacaaaaata aaactgaaag caattaaaat gaaaacaatc cagctgagct
    30541 tcggcataaa ttccaataca tcagattttg caagtgtaaa gccttccagt tcggacaggc
    30601 tgaactgcct ggtttctatc ttgtttctct tcacgaatat tttgtccctg gttatcagca
    30661 ttccggttcc ggcatcctcc agtatgcttt ccgtcacttg tcccgtagtg tcaataataa
    30721 aagcctcatt ggtgctgctg ctgatatagt agggcatatc tccttcaaag ctgaatacac
    30781 cgttctccag cctgaattcc ggaacatttt cagccagatg gacagccaga tcatccataa
    30841 atatccgggt tactatgaaa gttctggttc catttatgaa atagattatc agaaaaagca
    30901 gcagcagata aacgaagctc ctgctgaatt tattatccct aatgcttctg taaaacttga
    30961 aatcaattac gctttccctc atctgcacga aaaaattcat ttcatcgcct cccgcactcc
    31021 tgcaattcat atctatatgc caggataatt ataccaggaa taaccagcag atgctatccg
    31081 gcatattcaa tatattgcta aataccggtt atacttatag ctttaccgca tttgaagcat
    31141 ataccgtcat ccagatgctc tatgataatg cctcccgtcc ttcttataag caaggctccg
    31201 cactgcgggc aataagtatt tacagcctca ctttgcatat ttcctatgta cacatactcc
    31261 agataacttt cggccgcttt tttcaaagaa atcagagttc ctgccggggt aggctgctcc
    31321 ttcatcctgt atctcggaaa gtaacgggat aaatggagag gaattgaagg attgatagac
    31381 gacaaccatt ttgagagttc ctgaatctcc ctctcactgt cattgtgccc cggaacaaca
    31441 agagtagtaa tttctacgtg acattccgca gcagccgctt ctaccgtttt cttcacatgc
    31501 tcaaggctgc ccccgcaaat attgcggtaa tacttctccg taaatccttt gacatcaata
    31561 ttcatggcat caatataagg cagcagctca gaaagagctt ctctgcttat atatccgttt
    31621 gtgaccagca catttttgaa tccttcagac cttatatgcc ctgccgtatc caatatatac
    31681 tcaaaccata tactcggctc attgtaggta tatgccacac ctatacaatc ctcttccttc
    31741 ctgcataagc taagcagata agcctcagat gcttcgtaca gattatcctc tccgccctcc
    31801 tcagctgctc cccgctgtgc aatctcatag ttctggcaaa agctgcatct gagattgcag
    31861 ccaacagtac ctatagacag gatatatctg cccggaaaaa aatgataaag gggctttttc
    31921 tccatggggt ccattgcaat tgaagagatt tttccatagt tatgagtata gagaataccc
    31981 ccgatgttat tccttactct gcaaattccg tatttgcccg gtgcaatgtt acattcgtga
    32041 ggacagagcc tgcatttgac attgccccct tcttgtttct cataatgctt tgcttctttc
    32101 aattgaccca taatgaatca ctccatatac tgataatgcc ttaccacttc aaaacgctcc
    32161 attttataag gttcgccggg cctgatgcct gccttctgca gtgctatact cacttgataa
    32221 tcaacggtgt ccacgccttc cagattggga agcagcaatc cggatttgta cccgcttctc
    32281 acaataaccc catacctttt cacatcaagc tcatcttttg tattgatggg ctccggttcc
    32341 cccaatacat ccactgaata ggccaattca gaaagctcac tttcttctac cggtaaaaac
    32401 cgggggtctc tggttcccgc actgattgcg ttctggatga tttccgcagc aatatttttt
    32461 gtggtaggct ttatggtccc tatgcaacct ctgagttcgc cgtatttctt tagcgaaaca
    32521 aaaactcccg ccctgcgtgt ggtcatttca tcaggcaaac cttcaggcac atctattttg
    32581 cgccttgttt ttatataagt ctccagagta tctatagcca gctgcacata tggatcgctg
    32641 cttataacct tctttttatc cgcacacgcc tctgcgtgaa tctcagccac gcagtagcct
    32701 accccgaagg ggccttcgta ggaaaatacc tctcccttca cgctgcagcc atccagacag
    32761 cccataagca caacagtgga attgtaggca cattcccctg catcctccag aaggccggca
    32821 ggcatggaaa gcagtcctgt gacatctcct tctttaatga ttttcatata ctttttatca
    32881 tattcttctc ctcgggggtc aaaacctgcg ggagcatttt ttgtaagcct gtgggaaaga
    32941 tctccgcttg caattatgac tgcccttctg tcaagctcag ccacagcatc ccttatggca
    33001 atgccaaact tatagtgctg ctcccttgaa agaaggctgt agtttatatg caccagcttg
    33061 aaatgcctgt atttcttact cacaaaataa agggggacca tggcgccatg atctattccc
    33121 gacgcatatc tgtatgtatt ctttacatcc tcatgcatgg gaatgcaata tgcctgcttt
    33181 ttatcagcct gctccattat tttgtccgcc agctccaagt cattgtcaaa gctcagcttt
    33241 gtccccgggg ctccaaacct ggctaaactc ccctccagcc tttcatccgc cgcaataacc
    33301 attgcatctt tgagcatcgc accgtgaggt gttataagca ctataacatc cggttttatt
    33361 tcagcgatgc gctctgaggc ttccagagca ccgacaactg tgtttgaaac cttttttgtc
    33421 tcacttcccc caacttcctc caccataata ggaggatgcg gaaaaatgaa agcacctgct
    33481 atcttaccca tcttctttcc tccttctgac acttcatata tttccatttt tttaatattc
    33541 cccggctgat aatattaatc cttccgggac aaattgccgc acaatttttc tatttcaatt
    33601 atataatttt tgaataacta attcgattta aatttttaca tcattgctaa tttttgaaat
    33661 aattggagta atgatttccg gcacagcagc aataaggaat tgaaagtaaa attcttcagg
    33721 tatgataggc tgaacggctt gatattcggc agtgataata taccattaat aaaactgaga
    33781 tttttttcac aaaatagcga taaaaactat cgcatttctt tgatatatat tatataatga
    33841 taatgaatat tattatactt aatattttta ttctttaata gttaattata tacaaggagg
    33901 aatcattttg ccggatttaa aaagaatagt catactcggc gcaggctacg ctggagtcga
    33961 ggcagctaag gttctgggca agaagtttaa aaaggatgac agcgttgaga taactttaat
    34021 taatgataat cctttccaga ctcttcttac tgagctgcat gaaattgccg gacgtagaac
    34081 agaaaaggaa agtgtaatgg tagaccttta caaggtattt aaggctacca aagtcaattt
    34141 tgtgagggac agaatagaaa gcattgatta tgaagcaaaa actctgacat caagcaaagg
    34201 aacatacagc tatgattacc ttgtacttgg ctgcggagca gaacctgcat acttcggaat
    34261 cgaaggtgtt aaagaatatg gttatactat atggtccctt aaagacgctc tgaaaatcag
    34321 agagcaggtc ttaaagagct ttgaagctgc aagggatgaa aaagatgcag caaagagaaa
    34381 agaactgctt acctttgttg tagccggggc aggcttcacg ggaattgaaa ccgtaggtga
    34441 actcttcgaa tggaaagatg tattgtgtga tgaatatgga atcgattcga aagaagttac
    34501 aatatataat gttgaagcaa tgcctactat attgccgatt ttaaggccaa gccttcaggc
    34561 taaagctgca agctttctta caaaaaaagg cgtaaagcta atgacaaatt cacctatagt
    34621 caaagcaaca cctgacggta tcgttcttaa ggacggacag gagataaaaa caaagactct
    34681 tatctggact tgcggtgtac aaggcaacag tttcacggct tcatcaggcc tgactccggg
    34741 caagagaaac agagtgcagg ttaatgatta catgcagagc atggacaaag aagaagtata
    34801 tcttgtcggt gacaacgcat attatgaaat tgacggaaaa ccgataccgc agatagttga
    34861 aacggctatt cagacaggtg aatgtgcagc ccacaacata gcagcggata tagccaaaac
    34921 tgaaaagaaa aaattcaagc tcaacactca tggctttatg gtttccatcg gaagccacta
    34981 ctgtgtagcg gagcttatgg gcacaccctt atccggcttc attgccatgg ctatgaagca
    35041 ccttgttaac cttcactact tgtggggagt cggcggcgta aggctcatat ggaattacct
    35101 tctgcacgaa ttcttccata tgaagaacaa cagggctttt gtaggcggac acctggcaca
    35161 caggactcat acttcctggc tggtgcttct gcgtttatac gtcggcgtaa tgtggttcct
    35221 tgaaggttgg aaaaaggtac aggaaggctg gctgaacccg gctaatatca agatagtatc
    35281 ggttgcggga acttcaggct catcagcagc aggagcggca gcggaagccg taacaagcgc
    35341 ttcacaggca gcgggagctg ccggagaagc tgcagcggca gcagggccgg tgccgatact
    35401 ggcagaacct ctgggaatat atcagtggtt ccaggataca tttatcattg ctaatccgtt
    35461 cttcttccag gcagttgttg tattgatgga agttggaata ggccttgcac ttatggcagg
    35521 actctttaca ttctttgcat cactgggctc attattcctg actgcaaact ttattgtatc
    35581 tgcaatggca ggctacgaca tactctggta cgtattcgcc tccatcgctc ttatgggcgg
    35641 cgcaggcgga gcattcggcc ttgactatta tgtacagccc tggattaaaa aagcttggaa
    35701 aaaaacgaag tttgcccagg caagttattt gtacttcgat taaaatatta gaataaaaaa
    35761 caaacaccgg attttcggtg tttgtttttt atttgcgttc tatgaataat atcccattta
    35821 aatctagaaa ctattatctg atagtattcc aaaaacaatc taaaggagga tttcttgtgg
    35881 aagcaggagt aagaaacagg gtgtcaggtg aaatcgaagc aataaaagct gacgaaatca
    35941 tggcggaagt caagatgaga ggcacatgac aggacaatac catgagattc acgtccgtga
    36001 tgacgagaga aagccttgat gatgcgggtt tcaagcaggg cgacagagta gatgctctgg
    36061 ttaaagcaat caatgtagtc tttgtaaaac attaaagctt aagagggcaa atttccctct
    36121 taagctttat tcaaatactt tatgtttact tcatgttttc caatttctgc gttgatttga
    36181 catataactt gatttattat ataatatgca ttatgtaaac acaaacattt gctgaatccc
    36241 ggctttgccg ggtacggagg aaccaattct ggggttaatc catggacaac cgtggtaggg
    36301 aaccttctgt cccaaacccg acagctaact tcggaggcta gtaccttgta atacctgact
    36361 gttgcgttat gatacgcagg atatttgctt ccagtacaag gcgatggaaa gaggcgtagc
    36421 ggtggctgcg gcgattgaca tcaacgcagt agtggaacga atagacaagt atcatgcagc
    36481 ttttaggtgt tacaagggac tcaattatgg aaggagtgta ttgtgtatga aaaagaatct
    36541 taaaagcatt atttgtgtta ctattatttg tttgctttta gtcggacaaa ccataggcgt
    36601 ttttgcggat acctattacg gtttgttgaa attcggaagc agaggcactg ctgtagtaaa
    36661 attgcagcag gcattaataa acaagggctt ttcaccggga gcagccgatg gtatttacgg
    36721 caaaatgacg gaaaacgcgg tgattaaatt ccagcaggca aataagctca ggattgacgg
    36781 tattgcaggc agggaaactc aaagcaggct tttcgcggct gctgccgtcg cttcaagggg
    36841 gacaagctat acatcaacag atctgtattg gctttcaaga ataatcaatg ccgaagcgga
    36901 agctgaaccc tatgcaggga aggttgccgt cggtaatgtt attttgaaca gggtaaagtc
    36961 aggttcattc cccaataaag taaagggtgt aatattcgag tattataaag gcatccctca
    37021 gttcagcccg gttgcagacg gtactattta taatgatccg aatgcggaca gcataaaagc
    37081 tgcaaaagcc gcatttagcg ggacaagcta tgcgggaaat gcaacttatt tcttcaatcc
    37141 gaataaatca gaaggtacgt ggatcgtaaa taacaagacc tttgttaaaa gaattggtaa
    37201 ccatgtgttt tacaaataaa acttgaaaaa acggcatagc cgcagtaagc ggctgtgccg
    37261 tttttcgttg gcctaaggac agcatttgtg gtatcatatc aatagtagat attggcaaaa
    37321 aaaattccgg gtttatatag gaggcgtttt gggtgtgcgg atgtgaacat agaaggaacc
    37381 acgcaaattg tatagagagt gtgccgatat ttggaagcct gagccgtgag gagatgcttg
    37441 agatcgccaa tatcacctct gctaaaacct ttgaaaaagg cgatatggta tatatggccg
    37501 gcgataaaag caacaatctt tatgtgctgc ataccggcag agtgaagatt tcaaggctgg
    37561 gcaaaaacgg taaagagcag gtaatccgtg tgatagggcc gggtgagttt atgggcgaac
    37621 tctcactctt cgggtccttg cccttgaccg ataatgcaga ggtattagaa acctcaacta
    37681 tgtgcgtaat tgaaggatca aagcttaagg agcttatggc aaagtatact tccattgcct
    37741 ttaaggtgat gggtgaactg agtaaacgat tggaaaaggc ggaaaatctg atagaagcta
    37801 ttagcctgaa ttccgtagaa cagcgtttgg cacaggcttt actagcttta gctgacagga
    37861 aaaatgaggt tactttgaat atgagcaaag gagattttgc ctctcagatg ggaatgagcc
    37921 aggagacctt gagcagaaaa ctggctgtat tccaggcaga aggcataatt gagctgaaag
    37981 ggcataagaa aattatcatt ttgaacaaag ccggactgga ggaaatcagc gcagctgttt
    38041 agacacttgt tctcttccag aaggaaggca taccgagaag caagatggga tatatctcca
    38101 atctccctat aatcatacac attgataata ctatcttgct taaattcgac agtgtcgagt
    38161 agttgccaac cgcacctaca ataccgaaac cgggtcctat gtttccaata gtagcagcaa
    38221 cagatcctat tgttgttgcc caatcaagat tatcaaggga aataataaga acagccgcaa
    38281 taaaaattat aatatacatg aaaaagaatc ccaatacctc ggaaattgtt ctttcattca
    38341 ctgctctgcc gccaagcctg actgaataaa ccgcttttgg atgtattatt tgcagcagct
    38401 ctcttttcat aaccttgaac aatagcagaa atctgatatt ttttattgcg cccccggtgg
    38461 aacctgcaca tcccccgaca aacatcagca taaacagtat aattttactg aacattcccc
    38521 attcttcaaa attcgatgtt acatatccgg tagtactgat tattgataca acctgaaagg
    38581 atgaatgcct cagcgattcc cagacggagc tgaatacatt tccatacagg ttgattgtta
    38641 ttaagattat agctattagc actgttcgta aatagaaaag gaattcttca tccttaaaag
    38701 cagctttaag gtcgcccttc agcatctgat aatataaaga gaagttgaca ccgcatatga
    38761 acatgaatac tgctataata gcctcagcat acacattgtt atattctccg acgcttgtgt
    38821 tcatattgga aaatccgccc gttccgaccg taccaaaggt atgtatggca gaatcaaaaa
    38881 gaggcaatcc cgccattttc agcgcaatga tttccacaat agttatcaca atatatatac
    38941 catacaagat tttagctgtt tcagccacct tgggtacaat tttccccgga gtcggcccgg
    39001 ggctttccgc tttcatcatt tgcaatcctc cggcacctac tgaaggcaga atagctaagg
    39061 tgagtactat aacgcccatg ccgcctaccc agtgagtaaa gcttctccaa aaaagtattc
    39121 cctttggaag cctttctatt tgtgtaagta tgcttgcacc ggtagtcgta aatccggagc
    39181 ttgcctcgaa aaatgcgtca atgtatgaag gtatggcacc gctgatcaca aaaggaagcg
    39241 aaccgaagaa ggacagtagt atccatccta atgcaacaat ggcaaaaccg tcacgggcat
    39301 acataccatg cttgtttgct ttgatatagg ttaacggtat gccaataacc gcagtgatta
    39361 atatacttaa agcaaatgca gcaatatcat ctcctccaaa atataccgat accgccagtg
    39421 agggcagcat gaacaatgct tcacatgcca aaaccatgcc caagactctt aatactaatc
    39481 taaagttcaa tatcatatcc tccttaagaa accgcagcta cctcatccaa gctggagata
    39541 ttcttgtttt ttgaaatcac aattactcta tccccttctt tgataatatc atttccatga
    39601 gggattacaa cttcattttt cctgacgatg gtagcaataa tagtatcctt tgggagtccc
    39661 agtttcttga tcttcgtatt taatatctta ctttccggtt ctgcaatgat ttcagttatc
    39721 tccgcttgtc cctcaatgat tctgtataat gactcaacac tgttgccgcg aacatatttt
    39781 aatatctgat tggtggtaat ctgctttggg cttattacac tgtctatacc caggctttta
    39841 ataatgctta tatagttggt tctgcttgcc ttggatatga cctttttggc accattatgt
    39901 tttgctatca gtgcagccat catgttttcc tcatccattc cggtcactgc aataaacgca
    39961 tccatctcat caatattctc agatctcagc agttcttcat ctgttccatc cccatggatg
    40021 ataagtgctt cgggaatcaa ctcagataac tgaatacact tttctctgtc aatctcaatt
    40081 actttgacct tcatgcccat ctctgccaaa agctttccaa ggtaaacagc aattctccct
    40141 ccgcctacaa tcattacgtt tctaatctta tgaggaactt tgccgaacac cttgcaaaaa
    40201 ttgtacaggt gtgaaggctg ccctataaga taaatgctgt cattctcatt aattatgaaa
    40261 tccccgttgg gtacgataac ttcatcatcc cgtattacaa ctccaaccaa aacagaggat
    40321 tcaaacttac ttccgatgtc cttaaggcgc atgcccacca tgggcatatc ttccgcaact
    40381 tttatttcaa tcattttaac cctgcccttt gcaaagtttt caacattgat tgcagaagag
    40441 aagttcagca catttgcaat ttcatccgca gcagcctgct cgggatttat gataaggtca
    40501 agcccaagct cttctttgag gagcaaaagc tcatgtgcat attcatggtc tcttattctt
    40561 gctattgtat gtttaactcc caatttcttg gcggtgagac agcagaccat atttacttca
    40621 tcactgtttg tcacggcaat tagcaaatca gcagtctcta ttcccgcttc catgagaata
    40681 ttggtactta ctccgcttcc tttgatgcaa agcacttcaa ggttctcttc cgctttgctc
    40741 aaagcagcat aattcttgtc tattataatt acatcatgat tactctttga taagttttcc
    40801 gccagattat accctacttt tccggcgccg ataatgataa ttttcattcc ttaagccccc
    40861 atataaatgt ttttacttgc agcgttacat cctaaaaatt acactatttt caaagatgtt
    40921 gtcaaattgt tccttaagct cattgactat ctcaacctcg tcagtcatta ctgagcactc
    40981 aaaagtagta cttaacatat taaaactgtt cagtctgctg gagaaagaaa gtacaaatgc
    41041 atatctgcct atcatcagca cattgttggt catagaattg tttacccgca cctgtccttc
    41101 agccttgcac aagtttctta gcaaagtcag ggaaatgctg ctcttgaaaa tcagctttgg
    41161 tataatcact ctgcaagtac tgctgaattc tttatccata attaatccga ttatatcttc
    41221 atatagtgac tttgtccata ctccagaaac cataatatca agtttttgac tattgttcaa
    41281 tagggtttct acttcgtcaa taagctgctg tctggatgaa atatatcgaa tattgctccc
    41341 catggcacac ctccaaactt ttctcattaa aaataaatat aaaactataa tatcattcta
    41401 cagataatga cagctataag caagcttatc attagaagaa aacccctgcc tatttttgat
    41461 ttggcagggg ttttcttaca gaagctatgc gttttgaaat agagctgtgg atatatacct
    41521 ttctcccgta tccggaagca gtactacaat atttttgccg gcactgtcag gcctctttgc
    41581 gacttccgtt gccgcatgga gcgctgcccc cgaggaaata ccgaccaata taccctccgc
    41641 agccgcaagc tttctggccg ccgcaaaggc ttcctcgctt tttaccttaa taatctcatc
    41701 tataattgaa gcgttcaata cattcggaat aaaacctgcg cctataccct gcagcttgtg
    41761 gggacccggg gttccccccg acaataccgg tgaatcataa ggttccacag caattatcct
    41821 gatatcggga ttctttcctt tcagcacctc tccgactcct gtaacagttc cgccggtacc
    41881 gactccggca acaaatatat ctgcctgccc ctcggtatcc ctccagattt cttctgctgt
    41941 tgtattcctg tgagctgctg agttcgcggg attgttaaac tgctggggca taaaagcatt
    42001 cggaatctca gaaaccagct cctcagcttt ttttatggca cccttcatcc catcactgcc
    42061 gggggtaaga accagttctg ctcccaatgc tgcaagaagg cttcttcttt ctatgctcat
    42121 tgtttcaggc attgtaagta taagcctgta accccttgct gccgcaacaa gtgccagagc
    42181 tataccggta ttgccgcttg tgggctctat tattaccgta tccttgctaa caagccctct
    42241 atcttctgca tcctttatca ttgcatatcc taccctgtcc tttacgctgc ctcccggatt
    42301 gaaatattcc agctttgcta taagctttcc ttttatgccg ttttggctgt tatagttgac
    42361 ggtttccaga agcggtgtat taccgatgag ttcagtgatg ctttttgata cttttttcat
    42421 gacaatattc ctccatttta tactatttgt cctatttgaa tattaggttt tgcgttaaaa
    42481 tattatatgt aatacatata tgcttcattg cttgtcatct ttctatactc gtttgtcaaa
    42541 tcctccagtg taatgctgtc tatcactctg ccaatacttg agttaattgc actccatacc
    42601 ttttccctta tacattcctc aatcttgtca ttagtttttt cttccatgct tttttcgtca
    42661 ataactgaaa gatttccttc caaagccctc aatacagcac ctacagttat gtttgcaggt
    42721 ttatccgcca aagcataccc cccctgtgcc ccctttatac tattaatcag gcctgctttt
    42781 ctcagggttg agaagacctg ttccagatag ccctctgata tattctgtct ttcggctatg
    42841 cttttcaatg atatatgatc tcctgcagag tatacggaaa gatccaccat tgcccttagc
    42901 ccatatctgc cttttgtaga aatcttcatg tgcatctcct actattccaa tatgttttat
    42961 aatattacta ttatagcatc gctttttaag aattacaatg aaaaaatgca acttagttgc
    43021 atttttaatt aaagattatt ttattattgg tgacccatag gggattcgaa cccctgttac
    43081 cgccgtgaga gggcgatgtc ttgaccactt gaccaatggg ccttataaaa attactacgg
    43141 tactattata attgatacac atacttttgt aaatcgaaaa taattataat aagaccaggt
    43201 attattctat taataaaacc gtgcaatcca ataataatca cctatatttt gccttcactt
    43261 ccagcactcc ggatacggag ctgcctgcgt ccattgtgaa tgtgtctttg gttcccatag
    43321 aataggtaga agccataggt acattccgct atcataatga aaatatttct tcatatattg
    43381 ttagtgaatg aaaggaggtg cattatgctc attcatgttg taaaaccgac agaaacacta
    43441 tggcaaattg caaaccgcta tcgtgtctca actgcatcga tagcggaagt aaacgggctt
    43501 ccggatccga atcggctggt agtcggtcag gctttggtca tacctacgga ggatattttt
    43561 catactgtat taccgggaga aacattgtgg aaaatcgccc aagcatatgg aaccactgtt
    43621 caggcaattc ttcagaataa ccagattgcc aatcccgaca atatatatcc cggcctgcta
    43681 ttatatattc ccgctctccg ataccgtatc cagcccggtg atacattatg gtctgtggcc
    43741 cagaggtttg gggttccttt aaacagtctg ataaagataa ataatattga aaatgtgaat
    43801 cttgtttatc ccggtactgt attggtaatc ccccggaagc agaggcctcc aatcgatgtg
    43861 aacgggtata tctacatttt gggccaggag gctgttccga taattgagga ggcaggtgag
    43921 catctgacat acctaagccc cttcgcttac ttgataagag aggacggaag cctgcaggcc
    43981 attaatgaca tccctgcaat tcaagctgcc aaatcttatg acgccatacc catgatgtcc
    44041 attacaaatt ttacttcaac tgctttgggg gaaaaccttg cccatgtcgt attaaacagt
    44101 ccggcagtca tagaacggct gcttgccaat atcattaata tcatgaaagg aaaagggtac
    44161 tcgggattga atgtggactt tgaaaacgtg cttccggctg accgtgagcc ttataaccgc
    44221 ttccttcagc aagcggtaac ccgtttgcac caggagggct ttttcgtatc tactgcagta
    44281 gctccaaaaa caagcggcgt gcagacgggc ttgctgtatg aagcccatga ttatgaagcc
    44341 cacggcagaa tagttgattt tgtaattctg atgacgtatg aatggggctg gcgtaaaggc
    44401 cctccgcagg caatttcgcc tttgaatcag atacggcgtg ttcttgacta tgcagtcaca
    44461 gtcattccaa gagaaaagat ttttatgggc tttcagattt atgcaagaga ctggctgctt
    44521 cctttcgcct gggggcagga agcagaaacc ttcagtgtcc aggaggctgt atcaagggct
    44581 gtcagatacg gcgcctcaat acagtatgat acagcagccc aatcgccctt cttcaggtat
    44641 acggatgcgc aagggagggc acacgaagta tggtttgagg atgcccgcag cacccaggca
    44701 aaatttgata cggcaaaaaa ctataacctg cgggggatca gttattgggc tcttgggtat
    44761 cccttcccgc aaaactgggt attgttggag gataacttta caatcagaaa aggcgggtcg
    44821 taagcagaaa gaagaataat gcgacgcgga cgcggggaca cggggacacg gggacacggg
    44881 gacgtttccc ctgtcccact tccagtggga cacgcgaaac gtccccgtgt ccccgtgtcc
    44941 gtgtcgcatt attctattta tatgtatttc cctttatagt ataataatac atataatact
    45001 tttaaggggg tgccggtttt gtctgacctt aacaagttga agaatataag caatgtaaga
    45061 acctttaagg aaaacgaata tatattccat gagggagata ccggaaacga tatgtatatt
    45121 ctcttgtccg gcagagtgtc gattttcatc aaatccaagg ataatctcca aatcaagctg
    45181 tgcgacattt gcgcaggagg ttttttcgga gaaatgtccc tgcttgaggg ggagccccgt
    45241 actgctactg ctttaactat tgagaacaca gtagcccttt cagttaataa ggataacttt
    45301 gaagcatttg tagttgaaca gccaagtatg gcatttaagc tgctaaaagg cttaagctcg
    45361 agaataaggg cattggatga agagttaagc cggttaaaga agggaagtca atatgtcata
    45421 cccccggcgg attcaactga tgctttatca gacagcgccc cttgcatttc tgcctccaac
    45481 ccggaagatt tgaaaggcgt cctgtatcat gatgaacatg gaacttacga aggggaatcc
    45541 ccggatactg atactttcta tatctatgat aagaaaataa cctgtcccat atgcggaaag
    45601 gaattcgagc tgtcggttac atcccagtcc aagctcagac tcaactcgat tgatttcgat
    45661 ttcagacatc gatatgatgg atttgagcct ctgtggtata acgtcctgat atgtcctttc
    45721 tgttattttg ccggatattc caatgagttt gataatcata tggacaacct ctgcaaggca
    45781 ttcacggaaa aaacagcccc catccgcaaa aagctggctt ttaagtattc ccagccccgt
    45841 aagctggatg aggtttttac ggcttactat ctggctctgc acagtgcaaa agctctgagt
    45901 gccggtcctc ttaaaagcgc aaggatatgg cttcagctgt cctggcttta taaggatgcc
    45961 ggagatgaga aaatgtatgc tatcgcaaca gatcaagctt ttgagcacta ttacgatgta
    46021 ttgtacggaa gctccctgac gaagttgacc gacgaacagg atcaacagtg ctgcctcata
    46081 ttgggagagc tatatttgct gaaaaacgac cgcaatcaag ctgtgaggca attccatgcc
    46141 gcaataagga ggggcggagg aaacgcccta tacaacaaac aggcccagga caggatatac
    46201 tacgtaaaac acgaaatgaa ataggctgga cggggacagg gggacgggga cacggggacg
    46261 tttcccctgt cccacttcca gtgggacacg cgaaacgtcc ccatgtcccc gtccccctgt
    46321 ccccgtcgca ttcttattct attgccttct tctcctcctc catatacttc acaatgccca
    46381 cgaatatgct ccatgcaatc ttctcctgat agtgctcgtc atccagcagc cgttcctcct
    46441 cgcgatttga aagaaatccg cattctacaa gggctcccgg catattgttg ttttttagaa
    46501 tatacatgga atcagtggcc ttagcctttc tttcattccc tctgtccagc accttgatca
    46561 gttcattctg tatgccctcg gccaacttcc tggatttttc ctcattcttc atataaaagg
    46621 tctgggcccc aaaatattgg gactgtggaa agctgtttaa atgtatgctt ataagaatat
    46681 cagctccgca gctgtttatc agggcatgcc tgttcttgag gtcctcgctt tttcttgtcc
    46741 cctgtctgtt tccgccttca gtatagagcc cggaattatc ctctcttatc ataatagccg
    46801 tgccccctcc ctgctctatg agccttcgga gcttaagcgc aatcttcaga ttaacttcct
    46861 cttctgttat accgctgctg cccgacgctc caggatctct tcccccatgc cctgcatcta
    46921 taacaacaac agtacttcta atgggaaggg aaaatgccgc agtagtgctt ttgccgataa
    46981 aacatatgct tatgaacaac accaatatcc atgcaaattt gatcatatat ccgacagcct
    47041 tgcttctgtc gattatataa attctcactg aacacacccc caaatactcc ggcaaaactt
    47101 aaaataactg ttcagtttaa gatattcacc gaaccatgta caaaagaacc gaaagtatca
    47161 tggggttata tgggaagggc ttaaattgag ttgtgtaaat tgtgggattt gattgatgat
    47221 atgctttctc tcaacagacg tcctgcttca aaggcttttt gccgaatatc ctccctgcct
    47281 ctgacgtgtt gattatctac atcggagaca agcaggcttc cgacaaattc aacatttata
    47341 catttataaa gcagttcaaa aaccatgact gcacattcaa aacggctttt gccctcaggc
    47401 tctcctgcag tgcacacaat ataacctatc ctatgctttt ttgatatcag gctgccctct
    47461 gtcctgtact tgcttgccca tacagcctgg cacctgtcga taagcttctt catatgggaa
    47521 gataccgaat aaaagtatac cggcgatgat gaaaccacaa tatcagcttt atccatagct
    47581 tcataaacct gctgcatctc atcctttatc acacagtatc ccagctcgta gcatctctcg
    47641 caagccgtac agctctttat accctgcgaa gaagcatata tccgctctat gcttgcttca
    47701 ttgcttccaa taccctcaag tatcttatca gccagcatgt cactgttctg tccccgccta
    47761 tggcttccat agatagctaa tacgtttatc ataatatgta tatttccctt cctgatatta
    47821 gtgcatgtgt gctgctcgac tctcgtatct cgcacctcgt gcctcgtacc tgaccctgaa
    47881 gcatgaccct ttagcccttt atatatcctt tccctttaag cttctttata tggctctcaa
    47941 ccttctcctg tatatctatc gaatagcttt cttccagaac aaacatcatt gaaatcgcca
    48001 cctgggccac atccagcagc tcttcagcta ttctgtcaac gcattgtctt tcttccatcc
    48061 tgcatttttc tccgttcaac cctctgtact ttccgataag ctgtgccagt tcacctgcct
    48121 cttccatcag cttaagggct gtggactcta tattgggttt aaggttatta agcctcggca
    48181 ggctgatgct cttgtattcg gtatctccaa gtctgttcat taagaattcc tccgtaaata
    48241 ttttgttaac aacttatatg tattttttgc ttagccgcag tttcataaaa ctgtttacgt
    48301 ttctttttta actgttcttt aaaagtgaac atggatattg ccttaaaatc ttctattctc
    48361 atattaacaa atttgcagcc catttgaaac tttttttcgg agggtaattc attaactctt
    48421 agaactttag ctgtacaatc aataaaataa ttagtcttga gcttgaaact gaaaaccgag
    48481 tctaccggga gacataaatt tgtctctgcc aatacccctc ctgcgctgat attttttatc
    48541 gttcccaaaa atgtcttccc taactttcca acagagacat tatttatagt taccggtctg
    48601 gctacaaagc caatttcaca atctaccctg gcaaatcttc ttttttctat tgtatttaat
    48661 ttattttcta ttggtttttt tattataatc tcattgcctt caagataggt tatttcactg
    48721 taaaactcat atatatcatc attttcaaga aaaatacaca atatccccca tccgtttctc
    48781 aaaaaaccgg caggattcac actcagaatc agtgagtctt cagtaataga atttatatac
    48841 ccatatgctt cagaatcggt aaatggtgac aatataacaa gaaactgccc atttctaaac
    48901 tccataataa gcttacctcc tcccgtttaa gagcaactac taattagtat taagtaaaac
    48961 tttttttact attatatcaa atttcaatat aattcgacat ttaattgcta taatctgtca
    49021 ttttcattat atgtttggct cccaaccaaa caaacaacaa ttcatccggc tctcttatac
    49081 aacgctgaaa actcgtatct ccaatcaaaa catgcaaaaa gagaaggcat atatgccttc
    49141 tccaattccg taacaataat tatctctttg agaattgagg tgctcttctt gcttttttga
    49201 gcccgtattt ctttctttcc ttcattctag gatctcttgt aaggaatcct gcttttttaa
    49261 gcataggcct gagtgtatcg tcagcttcca aaagcgccct cgaaatgcca tgccttatgg
    49321 ctccggcctg gcctgtaaag ccgccgccga aaacatttac caatacattg aattttgaaa
    49381 gggtatccgt aagaatcaga ggcagcagat aatgcactaa cccaatggta gttgaagtat
    49441 ttgtatatag tatttgtata ggttgctcaa tgaatccgat gtttttcagc aaagtgttta
    49501 ttatgccggt gtttcccagt acgataaacc aggaataagc acgaatcaga tagccggtcc
    49561 agaacggcgc aatgatcagc accagaaaga agttgcgcca gcgtttcggg actgcgtaat
    49621 acaacaccat tgccagagga taggcaacaa gtgtagtaat agtcacaact ttaagggaaa
    49681 gtaaaaggga attcttcaac gccttccaat acataacact ggtgaaaatc ctctcatagc
    49741 tttggaaggt aaattcgctg gtaagcttat aatttcccat ggttttaaaa cttgttgcaa
    49801 cgatgaatag aagaggaata agatagaaaa atcccatcca gaatgaaatc ggcaggcacc
    49861 aaagagcgga gcggtttcct cccggacttg cattgcttgc attatttttc tttttcattt
    49921 taacaacacc tcccagtcta tgcctgcgcc ggcagtacca gcgcatcctg tgcagaaaat
    49981 gaaagggtta cccggtctcc cggttgtaaa gcagatttcc ctataacatc cgacaggaag
    50041 gtttcatctc cttcgtatct gagcatccag cgcacccaga ggttcatcca acagcatgac
    50101 aacaggatgc aacaccaagg atcgtgccaa ggctatacgc tgacgctgac cgccggaaag
    50161 ttgactgggt ttcctctttt cgtatccctg gggccctacc atattcagtg catgaaggat
    50221 tctttccctc gcttttttat gctctataaa catacatcca atttttctat catttaggca
    50281 cctttccaaa gcaaagagag gctggcagtg ccttccccct ttgtttttca aaagaatata
    50341 taagtataca tagatgtccc tacttgcttt tgaattcgtc ccagatagcc tgaacctcag
    50401 gagtcgtaag atactgtatg aatgccttgg ctgcttcctt gttttactgg cctttacgat
    50461 cgaaattgat tctgtccatg aaacgccgcc ttccttgggc actgtaactt cgatgggctt
    50521 gccttcttct ttcagaagaa cctggagcca ctcacccccg aagctgatcc atgcactttc
    50581 attggcaaga gcttgaattg tgtctgcatt ggtcgcagca atcataccaa cctgcggttt
    50641 cagctggttc aaggtatcct taacttttga aagttcatcc cctgaaatgt cgtagggttc
    50701 atcatagcct gcatatcggg aaagcattcc catattcggc aggtaccagt caaacagaac
    50761 aactttgccg gctgccttag ggttgtataa aaatgcagtg gatgaggcct cctcctctgt
    50821 aatataattg gagttgtata cgattccata atagccccaa cggctgacaa ctgcgtacag
    50881 cttatcccca accttcacct ggtcaaaatc cttccagcgg tcatagaaat tatcaatttc
    50941 cggataatct tcggattcag ctcatcaatc aggtctgcat ccaccagttt ggctacccaa
    51001 ggtgtatccg gagtaacaat atcccattcc ccctctgccg aattggcaag aagtgaaaac
    51061 atctgatcgg atgtagaata tacctttgca ttaattttga caccgtattt ttcctcaaag
    51121 ggttttatta tcttttcttc ctcatagcct ggcatcacaa gcaaattcaa ctcaccgccc
    51181 agctttccgc cttcctcggc cggtgccggc tccacgggtt tgttctccta gcatccgctt
    51241 aataccggca gcagcatcgt cagtaatact acaaaaccaa cgcctttttc attatttctt
    51301 cctcccatac tgaatatttt gctgttcctt tctctacgcc gtaacaatta tcttgctgta
    51361 tataatactt tcaagtgctt ttcgacacta taccaagcgt cagagtttga actatatgta
    51421 tacatatact accgtatgca tatatatgtc aggaggatat tatctgtatt attattattt
    51481 ttcctttgcc gcccatgggc atttcccgaa ggaaacctcg gtgcgagaag catacttttc
    51541 tatattatac aaagtccctc aggcacaatt gcctgaagga ctttgtattt tgcaaaccct
    51601 aacgttttga gaattgagac ggggcagcgg ggcaccaagg caggtatctc gttacaacct
    51661 gaaaatttca aagtagttct ttttaaatgt aattagccct tccttttcca tccgcttcaa
    51721 ttcatgagaa agcacgctgc gattcatacc taagaagttg gctaactttt cacgatcaaa
    51781 cttgatatag aattgattgc tcccattgat tttctgttgc aaagctaagt aatgtacaat
    51841 acgctcccta atacctttta tggaaattgc ttcaatttta tgatacttac gcatgttttc
    51901 atcagcaata tagagcatga tattccgtag tagaatttct ctgattccat cgtcaattaa
    51961 tccattttta gttattttct caatagaaaa ccaatatata tccgtatctt tctcgcaata
    52021 aatagtataa ggggcgtttt gttttctggt aaatgcgact tcagcacaaa caagatagaa
    52081 tggaacaagt ttttgcatga gaagttcctt accatccatc gtgaactttg aaatatatgc
    52141 atttccctga agaagtattc caatccggtt tacgattgca tcttcctcaa caatcacctc
    52201 ggcttcccgg tacgaattaa ttacgccatc taactgttgt tttgccaatt tgatctgtcg
    52261 gtctgataag ccggaaaata atctgcaatt ttgcaaatct tacacccctt tcccaactta
    52321 ctccgtatgc cgtcggcaat ctgccgacac ttttctttgc agagtcaata cataatatag
    52381 cataggttac ccccaaaact caagaaaagt ttgcaatatt tacctgtttc aaaatatgaa
    52441 aactacccca ctaaagtttc ggtgctcctg cggggtcatt cttttgtagt tgtgacttac
    52501 tcaatgatct cggaaatttg ctgtggagta agatcatatt taaagaaatg gctgtaaaaa
    52561 tagcttactt tttctgccag catgttatca gaaaccagct cgggataaag cttgttgagc
    52621 gtccagaaaa cggttagagg ctgctctacc gtacggttgc cccaagtgtg ggcaactgtg
    52681 gggatataat agatgctacc cgcctttaca gcagttacat ctgtataaag cttatcttct
    52741 ttgatcttat caatcatgtc acgctcggtg acgattatta tatccggatc ccacagcaat
    52801 agctcctctt gagtatattc acgggattga ccggtggttt taccgtcgtt tgttacactt
    52861 atgccgcctg cgacatctat ccaccactca gcaataacat gtggctgggt gagattggta
    52921 acattgccat agagaacggt tttcttgtct tcatcggtga gcttggcggt caaatcggaa
    52981 gcttcttgca ccatagaatc aaaataagcc aaatagtcct cagcgatatc ttctttttta
    53041 aggatctcgc ccatcagatt gacggcagcc ttcacatctt cagtctgttt ccattccaga
    53101 tatatacagg ggataccgtt ttcaccaagc tgttctgcag tttccttggt catagtaata
    53161 caaagatcgg gttgaagctt tagaacctcc tcaaggagca gttcgtcgtt actgttagcc
    53221 agcagtggtg cgtccttaat ctgcggcgca aactcatact gcattttcca tttgtcggat
    53281 ttggtgaatc ttgccggcat ctggttgcac agcagatcac cactgcccat cagctctaca
    53341 aaagcattaa gtacgccaac tgagccgaat gtagcgatag acttgatctc gccgggaatc
    53401 gtcacctggc ggccggccat atcagtcaca gtaaaatcgg taatgacatc cggctgcgag
    53461 ttggcgggcg cacaaccgga gaatgcgacc atcaacataa taatggccat agtaagtgct
    53521 aataaacgtt tcatcttaat cctccttaat tgtaagatat ataatcgcac acatcctgtc
    53581 gttaggacat ggatggaatg caaactttcg attcttgcgt acctgaatta aaaacagcct
    53641 gagcgtctgc tacgtataca tctaacccgt atagtgaaga taggttccgt gacgtaataa
    53701 tgtcttcagg ggttccgtca gcaaaaacaa tgccatcttt gagcatgagt acccgcgtgg
    53761 cagctagaaa agcctgatcc ggcaagtgtg aagtcatgag aatagcataa ccatcctccg
    53821 caagttgtct gatcacctgc aagatacgga tttgattcgc atagtcgagg ctggcggtag
    53881 gctcatctaa aataaggcaa tccgtctcct gtgcaagagc acgtgctata agaacaagct
    53941 ggcgttcacc accgcttagc tcgtggaaac ggcactgcgc catgcacaat acattgagcc
    54001 gttccatggc agcttcggca gctattctgt ctttaaggga aacagatgcc cctaacttct
    54061 gatgtgccac acggcccatc aaaactgttt cccatgcagt gtagggaaaa gtaagtgccg
    54121 acgcttgcgg tacataggag atcagtcggg cgcggactct cgggcgtaaa gaagcagcat
    54181 ctttcccatc tataaaaatt tttcctccct gtatgctatg cagattaagc aaacagcgaa
    54241 acagtgtagt ctttccggta ccgttcggtc ctaatacgca aagactttca cctctctcaa
    54301 agttgaagga cacgtcccgt agtaccggtg cactgtgccg atagctaaat atcacattct
    54361 cgactctaat catgtcacca ccccctttta atccgggcca gcattacggc aaaagcagga
    54421 gtgccgatga gagctgtcat aacaccaaga ggcaattcca ctcctgggat accccggatg
    54481 atattatcaa tcagcagtaa aaaaagtgcg ccgatcagaa atgaaaggga acacaatctc
    54541 ccgtaactcg cacctgtgag tgcacgggca atgtgaggaa ctaccattcc tacccaaccg
    54601 atgataccgc atatgctcac agaaactacc gtgagcaatg tggaactgac gataaccaca
    54661 attttcacca aaggcacatt gacacccatg cttaaggctt catcctctcc tgcagacagt
    54721 gcgtcgatat gtgatcgaaa aagaaataaa acaaaagcgg aagctgtcac caccgggagc
    54781 attatgagca cgtcatcatt gttacccttt ccaaggctcc ccatcaacca gaatgttatt
    54841 gaaggaagtt ggttatcagt atcggctaag gttttgacaa ctgaaagcag agattggaat
    54901 agactggacg ttacaatacc tgccagtatg agaaccgtta aattctgtcg tccaccactg
    54961 agggatgcga ttaggtacgc aagccctaca gccaaaagac caaatgtaag tgcaaaaaac
    55021 tgtataccag cccatgagcc gttttgaagc atagctaaag ccgcaccaaa gcctgcgcct
    55081 gccgatacgc ccaaaatatc cggcgagacc ataggattct taaacaatgt ttgataagac
    55141 gctcctgcca tggaaaggga tccgcccaca aggatggcca gcaggatgcg gggcaagcgc
    55201 acatgcagca ccaccttttc aagtgtaccc tcccagtatg ccgttatggt aaaaaagcgc
    55261 gacaggataa tatctgccac cgttctaata gcgattggat accgaccgat aaggaatgag
    55321 atgaaaaagg cggcaaccaa cccgcatagg aacatgacca tcatcagagg gtaacgaatc
    55381 tgtttcttca tggctgattc ttaaatatca cggttctctc tgccgatttg ccgaagaaat
    55441 gatatccgga acccccttcg gatatgatat ctaaaagctc gtccgccttt gtcacaagga
    55501 tgccgcccac catggtaact ccccgtgaga acagtgcatc cggcagcatg ctggctgtgg
    55561 gaccggttac gataatctgt gcgccgggct tagccatatt caatatgccg ggaagggtat
    55621 cattgagaat ggtcacgccg gtaatgacga gaaggtcggc atccggcaca tacttatgtg
    55681 catcctcagc gggagcgtaa taaggcagct caccggattt aagcgtgcgg ggatcctgtt
    55741 cgagaacgcg aaaatcacag cctccggtta tcagcttttt gagcataggt acaagtgcgc
    55801 cgatgacaac ggacttttta tattgtgaaa catccacatt atcaaatgca tccataccga
    55861 ttgcaataga atatccttcc ggcggctcca actcccatat caagttgctc aatgcattga
    55921 gagcagcaat gcccagagtc tttttgagaa tgttgtcact gaacacatct tccaaatatt
    55981 gctctgcact gcgtccccgc agttttcctg acaacggcat ggcagctgcc gaactggggc
    56041 agcataccgc tgcagggata tccttaacag gtgtgaagca tagcccccca tgtccggtgg
    56101 acagcttgac cccggagaag aaaagaccca ataccgccct atctatggta atattcgaaa
    56161 gttctgattt tagacgccct tttactatat gtacagattc aagtaatatt tcatttttcg
    56221 acatgacgta ttcctccttg tgcattgtca tgattagata tagctgaact cgttctgttt
    56281 tctctgcaat ccattacccg tatttcaaat atcaaagcca ctcagcaact ctgcacgcac
    56341 catttctcct gcttcaaaag tctccttgcc aatgggagca ataaacagtg cattacaatc
    56401 tgccaacagt tgtgccaagc ccttctgggg gctgagaacc tcagcagtat accggccatt
    56461 tatacatttc actcgcagcc gtacatacat ctccaaatgc gatggctttt gcactttttg
    56521 cttcagaaca gccctgacgg ttctgcgtaa aggcatgtcc tgcttcagca tatggaaaac
    56581 cattgcctta acgcaccaat ccatagcata gtaggatgcg agggtaggcc ccggcaggtt
    56641 gatgacaggc tttttgtcaa tgatagccaa tgcaatcggg atacctggga tacaacgtat
    56701 accatgctga aaccattcgg catgtttttg cagcagacgg ctggcgtaat cttcgctgcc
    56761 catagatgat cctccgttta ttaagacgat atcggctttt gagcatgcat tcagcagtgt
    56821 ttcttccatg agtacgttat catccttaac gatagggcag cagtgcgcct ttgcacccca
    56881 ttgcgcaagc atggtccgta ccattagact gttgctttcg atattttggc ctcgcttcgg
    56941 agacacgcca atgggaatca attcattgcc tgtaggaatg aatgagacaa caggtttttt
    57001 ataaaccttt accgtactaa ccccggcagt gctcaggaaa gccaaatgta caggctgcaa
    57061 aagggtgtcc tttttcagca gctgctgccc ttccttcaat gtatccccgc gcggcctaat
    57121 attttgtccc tgttttattg gttccttgga agtaatggtt agcgatccat ccgaatgtct
    57181 tacgattctt tcaacggcaa tcaccgtatc gaaggcatca tcaaaatcat cgccggtatc
    57241 cgctgataca tgggcaattg tgcctgtcca ggggaattga cccccactgg cttgcattag
    57301 cgcctcaaag gatacagcga tgccgtcaag cttggaactc ctgcataccg gcaaagtatt
    57361 cacagaactg acagcatcgg aaagcacacg tccgactgct tccgataccg caatgctctc
    57421 tgtggctatc tccggattcc aacgtccaaa aagctcctta agtgcctcag agcgcataag
    57481 aaattgcttc tgattcaggt acatattaaa aacctcctgt cccggtactt tattgcttat
    57541 atggttttat ttcctcagat tagtgaccac acaaagactt tattatcatt atctactaaa
    57601 tcctctataa tgtaagttgt tataacaaca tttttaatga tccggggagg tgttaacatt
    57661 gagcggattc gcaaatccgg tacgggcagg gacaggtggg acaaggtgcc tgtccatctg
    57721 cccaataaaa aaacgccaga ccgtttccaa tctgacgctt gtgttttgaa attatctctt
    57781 tgagaattga ggtgctcttc ttgctttttt gagcccgtat ttctttcttt ccttcattct
    57841 aggatctctt gtaaggaatc ctgctttttt aagcataggc ctgagtgtat cgtcagcttc
    57901 caaaagcgcc ctcgaaatgc catgccttat ggctccggcc tggcctgtaa agccgccgcc
    57961 gataacattt accaatacat tgaattttga aagggtatcc gtaagaatca gaggctgtcg
    58021 cacaataagc ttcaatgtct ctaatccaaa atagtcatct atttctctat cattaattat
    58081 tatcttacct tctccgggaa ccagtctgac tctagcaaca gactgctttc ttcttcctgt
    58141 tccaaaatac tgaactttct ttgccacagt aacttcctcc tttcaaacct atatgtccag
    58201 ttcaagagct tccggcatct gagctgcgtt ttcatgttcg gcgcctttat aaactcttaa
    58261 ctttttaagc atttttctgc ccaagctgtt tttaggaagc atgcctttaa ctgcttcata
    58321 tacggccttg tcagactgtt cagccatgaa ctttctgtat ggagtctcct tcatgctgcc
    58381 gacacgggtt gttactgtcc tgtgcatctt ctggtcaagc ttcttgcctg taagtacaac
    58441 cttgtcgcag tttataacta ttacgtaatc gcccgtatca atatgaggcg tataaatcgg
    58501 tttatttttt cctgttaata tctttgcaac ttcacttgca agtcttccga gaaccttatc
    58561 ggttgcatca actacatacc attttctctg aatctcatcg gtctttggta tatatgactt
    58621 cattttttcc actccttgtc tataatatta tgacagatgt gaactttaca gcatgcaatt
    58681 gtgttccaca ttcacaaaag ccggctgccg attcgttcaa ataagcacac taaatttttc
    58741 tatttttcaa gtttatttat tctgccaata ttaatacatt gcaagtgatt ggggctaaga
    58801 atcacttttc gcaacatata atattttaat acattgaacc acgtgtgtca agacaaatta
    58861 tagtatacct cttccaaata cagaccccgt gccggaacgg ttataccagc ctgttccctg
    58921 ttctttgaca gtattacgtc cttcatatcc tccggattga tttttccaat tccggcatac
    58981 agcagggtgc ctgttattat acgaatcata ttgtacaaaa atccgctgcc cttgtaaata
    59041 aaacgaatta attccccttc ctgctctatg gaaatatcat atattgtcct gacagtattg
    59101 gaaaccttgc tgcccgctgc catgaacccg gaaaaatcat aagtacctat aaaagcttct
    59161 gccgcagtct ttaccttgtc tatatctaat ctttcacaat agttgacatg atatgcatga
    59221 tttctaagaa gcgcagaagg aaatctgctg tttataacga gataactgta cttttttccg
    59281 acagcgctaa atcttgcatg aaaatcaagg ggcacttctc ccgattcctt tataacaatg
    59341 tcttccggca gcttgttgtt caaagcatag gcaaacttct ccggaggtat ggaactttcc
    59401 gtgataaagc ttgccgcctg ccctctggca tgaactcccg catccgtcct gccggagccg
    59461 tttatgctta ttgcctcacc ggttacggaa agcagagcct tttcaatttc agcctgaatg
    59521 gaaggcccat tctcctgaac ctgccatccg cagtaattcg tcccgtcata ttctataatt
    59581 aattttatgt ttctcatata tatcccctcg cgcctcgtac cccgtgcctc gagccaatac
    59641 cccatcgccc atccctaggg tcatctgctc catatcacca gaaagcaaaa aactgctata
    59701 atcatcatgg cactgtaatc tctgaattcc agcttcagct gcttcattct cgtccttttc
    59761 tctccgcccc tgtagcacct tgcctccatt gcaagagcaa gctcatcggc cctcctgaat
    59821 gcgctgatga ataacggaac caacagcgga accattgctt tagccctatt tatcagattg
    59881 cccgtctcaa aatccgcacc tcttgccatt tgcgccttca taatcttgtc tgtttcctca
    59941 agaagggtag gaataaatct caaggcaatg gtcatcatca tggccagctc atgagcaggt
    60001 actccgattc tctttaaagg attcagcagc ttctcaattc catcggttaa agctatcggc
    60061 gatgttgtca gtgtcaaaag agaggctccg gttatgagga atatcagcct cagccccata
    60121 aatcccgctt gatacaaacc ttccttggtg attgaaagcc cccagatcct gtatattact
    60181 tctccttgtg tcataaaagc attcaatatc accgtcaaga caattataac aatcaacggc
    60241 ttcaggccct tggcaataaa cttcagggat atctttgaaa gcccaatagc caaacccatg
    60301 aacgcaataa tataaaggta agaaaagtaa ttcccgacta aaaacaataa tgttataaag
    60361 acaaaggtta taattatttt tatcctggga tccaggttat ggacggctgt atctccggga
    60421 atgtattgtc ctattgtaat gtccttaatc atattctatt acctcttaaa aagcttcagc
    60481 agctcatttt ttgcttcatc tatatttatg caatcagcaa tggggtaccc cttttttctg
    60541 agttctctta ccaggtatgt catttgagga acacccagcc cgattttttc caaggtttct
    60601 gcttctccga aaacatttct tggtgtatca aagtatgcta ttttgccttt attcataacc
    60661 aataccttgt ccaccaggtt agcaatatct tccatgctgt gcgacaccag cactactgtt
    60721 attttctgcg tctcatgaag tgtcttgaac cccttgagta tatcatccct tcccttgggg
    60781 tccaaacctg cggcaggttc atccaatatc agcacttttg gctccatcgc cagcactccc
    60841 gctattgcaa cccttctctt ttgccctccg ctgagttcaa agggcgatac attgcttacg
    60901 ctgtcaaaat ccaatcctac aagctcaatt gctttacgga ctcttgcatc aacctcatct
    60961 tcacttaaac ccaggttccg ggggccgaaa gccacgtctt tatacaccgt ctcttcaaaa
    61021 agttggtact cggggtattg gaaaaccaaa cctaccttct gtctgatttt cttcatctgt
    61081 acttccttgg agcccagttc ttccccatcg atgaatacct ttccgctgct gggcttcaat
    61141 agtccgttta aatgctgtat gagtgtcgac ttccctgagc ccgtatggcc aataaggcct
    61201 atgaattccc cgtcttgtat ctcaaagctt atattgtcta ttgcaacatg ttcgaataca
    61261 gagtctttca tatatatatg ggtaaggttc tctactttaa ccggcacaat tccaccacca
    61321 tttcttcaac agtcagaata tccggagaaa gctgcagccc ctccctgttc agcctgtgac
    61381 tcagttctgt aacctgagga acatcaagtc ctgcggcttt taccgtttcc acttccttga
    61441 atacatcctt aggctttcct tccataataa tcttgccgtt tttcattaca tatatcctgt
    61501 ccgcctgtat ggcttcatcc atgtagtggg taatgaggat tatggttata ccttcctcct
    61561 tattgagctt tataatggta ttcagcactt ccttccttcc aaccggatca agaagtgcag
    61621 taggttcatc aagtatgatg cattccggct tcatggcaat aatccctgct atggccactc
    61681 tctgcttctg gcctcccgac agcaggtgcg ggctgtgctt ggcatattcg gtcatatcta
    61741 ccgtttttat tgagtcgtct acccttcttc ttatttccaa aggcgggatc ccaagattct
    61801 caggcccgaa ggctacatct tcttcaacta tggtagctac tatctggttg tcagggttct
    61861 gaaacaccat tcccgcagtc tgtctgatat cccataagag cttttcattc ttggtgttca
    61921 tttcttttac gtatacagta cctgctccgg gcagcagcag ggcattaaag tgttttgcca
    61981 gggtagactt acctgaccca ttgtgcccaa gtatagccac aaactcaccc ttggcaatat
    62041 taatatttat atcatccaaa gccggtttgg cgcttccgct gttagtcccg tattcaaatc
    62101 ttacgttttc agtggtaatc atatttgcca tttttaattc cttctttttc ttccgagcag
    62161 tcgtttagtc tgcccaacct atagatattc ttcgaatcca gtctttaacc cctcgtgtcc
    62221 cgaacctcga acctcgtgcc ggcgttttgc gcacaaaacg cctcatagca aaaggggact
    62281 aagcagtcac ttaatcccta ataattcgat atgatctacg cttctacacc agttccagta
    62341 caactacttc cgctgcatcg ccgcgtctcg gagcaacctt cgctattctt gtatatccgc
    62401 cgtttctctc cttgtacttc ggcgctatat catcaaagag tttttttaca acatcttctt
    62461 ttgtaatata tcccaaggcc tgacgtcttg catgcaggtc accgcgcttg ccaagtgtaa
    62521 tcattttttc ggcaatgctc tgagtttcct tcgctctggt ctcggtagtc acaatctttc
    62581 catgctcaag aaaagaagta accaagttcc taagcatggc tttcctatga tctgaaggac
    62641 gtcccagctt tctgtatcca gccattgcaa tacctccttt tgttttgtat tgttaatcaa
    62701 tatccgtatt aaactgctta cgcaccagaa tcctagtcct cgctcgtttt taagcttaat
    62761 cccaaggctt caagcttctg ctgcacttct tccaatgatt tcttacccag atttcttacc
    62821 ttcatcatat cttcctctgt cctctgggtc agctcctcaa ctgtatttat tccggctctc
    62881 ttcaggcagt taaaggatct gaccgaaaga tccagctctt caatggtcat ttcaagaacc
    62941 ttttccttct tgtcttcttc tttttccacc attatttcaa cattgccaac atgctcagtc
    63001 aaggttatga ataacttcaa gtgctcgctg agtattttgg caccaaagct tattgcctca
    63061 tccgggttga ttgtaccgtc tgtccagacc tcaagcacta atttgtcaaa atcagtaacc
    63121 tgtccgactc ttgtattctc taccagatag ttgacctttc tgaccggcgt gaaaatcgaa
    63181 tctatcggga ttattccgat tggctgtccc ggctgtttgt tcttttccga tatctgatat
    63241 cctctgccca tttccaccgt catttccata tataacctat ggccttcatc aaccgttgct
    63301 atatgaagat ccttgttaat aatttcaaca tcggcatcac atatgatatc ggaagcttta
    63361 atttcctttt caccctgaac atctattttg ataatcttgg tattttcccc gtgcatctta
    63421 agcgcaagat tcttgatgtt taatattatt tccgtcacgt cttccgctac accgggaatt
    63481 gttgaaaact catgaagcac accgtcaatc ttaatggaag ttacagcaac tcccggaagt
    63541 gacgaaatca ataccctcct taaagagttt ccaagggtag taccgtaacc tctttccaga
    63601 ggctccacta cgaacttccc gaaagtataa tcctccgaca tttcgataca ttcaattttg
    63661 ggcttttcta tttcaatcat cctacaaacc ctccttttat ctatggcttt tttattctga
    63721 gggcaactga tacgtcaagt ttatataaag aaacttcatc ttctatcgct tatgctgcac
    63781 taaaacttat tatcttgaat acaactcgac tattagatgc tcttctatcg gaaggtcaat
    63841 atcctctctg gccggaagag caactattct tgcttccatc atctccgtat ttatagataa
    63901 ccactgtgga gcagttttcc ctgctgcgtt ttccgcaagt cccttgaact tgtcagtact
    63961 cttgctcccg ggttttacag ctatcagatc gttaagacct gtctgatagg atggaatatc
    64021 taccttcctg ccgttaactg taaaatggcc atgtctaacc aattgtctgg cttcggctct
    64081 tgattcaccg aatcccattc tgtaagccac attgtcaagt ctgagctcaa gaagtctcaa
    64141 aaggttctca cctgttacac ccttctgtct ttcagcttca acaaagtagt ctctgaattg
    64201 cttctcaagt attccgtata ttcttcttac tttttgtttt tctctaagct gtattccata
    64261 ctctgacacc tttttcctgc cctgtccgtg ctgacccgga gcgtatgccc ttcttgcaac
    64321 agcacattta tccgtataac agcggtcacc cttcaaatac agcttggatc cttctcttct
    64381 gcaaagcctg cacttagcgt ctgtatatct tgccatctat cacacctcct caataactaa
    64441 actcttctgc gttttggcgg tctgcaaccg ttgtgaggta taggagtaac atcttttata
    64501 aggctgactt ccagtcctgc agcctgaagt gatcttatag ctgcttctct gcctgcgcca
    64561 ggtcctttta cgaatacctc tattattttt agaccatgtt ccattgcacc ttttgcagcg
    64621 acttcggctg ccatctgtgc agcaaagggt gtgctcttct ttgatcctct aaagccaagt
    64681 gaaccggcac tggaccaaga caatgcattc cctgtgctgt ctgtcagggt aactatggta
    64741 ttgttaaaag tagactgtat atgtgcagct cctctttcaa cattttttct ttcttttctt
    64801 cttctggttt tcttagcctt tggctttgtt accattaaag caaacctcct tatttaccaa
    64861 tttactttct tcttacacca acagtcttct tcggaccttt ccttgtcctc gcatttgtct
    64921 tggttctctg ccctcttaca ggcatacctc ttctgtggcg taatcccctg tagcatccta
    64981 tttccattaa tcttttgata tttaaagcta tatctctctt aaggtcacct tcaactttaa
    65041 cgcttctgtc gattacttcc cttaacctgc ctatttctgc ttcagtaaga tcctttattc
    65101 ttgtgtcagg acttacacct gcttctgcca aaattctgtt tgatgttgct cttccaatgc
    65161 caaatatata tgtgagccca atttctgctc ttttgtctct cggtaagtca acaccggcaa
    65221 ttctcgccat tagtacttgc acctcctaat tgctttttgt ttatactaaa atcaatcggg
    65281 aacacccatt tatcatgggc cagccgcgtc ccccgaactg gttttatcct tgtttctgtt
    65341 tatgtctagg attttcacag ataaccatta ctttgccttt tcttctgatt atcttacact
    65401 tttcacatat aggctttact gatggtctga ctttcatctt aaaccctccc tgtatcattt
    65461 gcctcgccaa gtgatcctgc ctctggtaag atcataaggc gataactcta ttgtaacttt
    65521 atctccgggc aaaatcttaa taaaattcat tctgagcttg ccggatatat gtgctaaaat
    65581 tttatgaccg ttttccagct ctacctgaaa cattgcattt ggtaatgcct caactacttt
    65641 tccttctacc tctattacat cctgctttga cataaggtaa tcaaacctcc cgttcccgat
    65701 atcaatcaat agattttaac aatttcctga tctcgctgtt actgagtttc tcacaagatt
    65761 ccagtttttc tttaatattc tcggcaacat gattcagctt ttttagatgc tttactttct
    65821 taagcttagg cttatctact tttcttatgt ccccatcaga aatatagaca tacttttcat
    65881 caattataca taaaacaact aatttgcagc ctttatcccg tcctgcggct gatattacta
    65941 tctgtcccaa acttatatcc tgcatatatt caccttccga acagctacaa aagcgtcagt
    66001 atttcaggct tgccatttgt aatgacaatc gtattctcat aatgtgccga tggttttccg
    66061 tcagccgtta ccactgtcca accgtcttcc cggaccttta ctgcatattt gccctgattt
    66121 accatgggct caattgcaag cgccataccg gccgctaatc tgggtccctt gtgcggcagc
    66181 ccgtaattag gtatctgcgg atcttcatgc atgtttcttc ctattccatg cccgacaaaa
    66241 tccctgacta cagaaaatcc gtttgactcc acgtgttttt gtatgctatg ggaaatatca
    66301 gacaatcggt tacctggcag agcgtattca atccctttga aaaaactctc ttcagttacc
    66361 tttaaaagcc tttccgtttc tttatcaatg ctccctatcc agtaggtcct ggctgcatcc
    66421 ccataatatc cgtcataaac ggctccgata tctatgccta ttatatctcc gtccttcaac
    66481 gctgtaggcc ccggtattcc gtgtacgacc tgctcattta ttgatgaaca tatgctggct
    66541 ggaaatccgt tataatcttt gaatgcaggt attgctcctc ttttccttat aaactcttct
    66601 gcaattgtat caagttccaa tgtagtaata cccggtctga ttgccttact caaaagctca
    66661 tgggtttccg caactatcct gcccgctatt cgcatcaact cgatttctct gggagacttt
    66721 atgctaatca tttaaatctc tccaataccc tgcagatgtc tccatatact ttatcggcag
    66781 gctgttcacc atttacagaa atcagaattc cttttccttt gtaatattca ataagaggga
    66841 atgtctggtt tttatataca ttcaaacggt tcatgactgt ttcgggctta tcatcttccc
    66901 tgatataaag cttccctccg cacttgtcgc aaattccatc cgccgcgggc tttaaaaagc
    66961 atagattata gctttctccg caatcactgc atattctcct gccggccatt ctttcaataa
    67021 ttgtttcggc ggatacttcc aggttcacaa ctgcgtcaag tttttttccc attttttcaa
    67081 ggtccttgtc taaagcttca gcctgagcta tgtttctcgg aaaaccgtcc aacaggaatc
    67141 cttcattgca gtcctcttca agaagcctgc tctcaacaat gcttacagtc aaactgtccg
    67201 gaaccaataa acctctctca ataaacttct ttgcttctgc tccgatggct gtatttctgc
    67261 tgatattgtc tctgaatata tccccggtgg atatgtgcgg tattccaaac tccttttcta
    67321 taaagctcgc ttgtgtacct ttgccggatc ccggcgaacc caacagcact atcctcattt
    67381 ctacctctcc aatccggagt tgtgcagaat tttacacaac tgcgttcaat ataaacctaa
    67441 tttattttaa gaatccctgg taattcctca tcaccatctg cgcttccacc tgctttacga
    67501 aatccattgc aacaccgaca actatgagca gtgaagtgct tccgaaatac accttgatac
    67561 ctgtaaagtt cataaggaat atcggaacta tagctattac agcgaggaaa aatgctccta
    67621 ccaacgtaat tctgttaagt gattttgtta taaaatcgga ggtcggtttt cccggcctga
    67681 ttcccggtat aaatccgcca tacttcttga tattgttagc gacctctata ggattaaagg
    67741 atactgctgt atagaagtat gtgaatccaa taatcagcaa tgcatatagt atgctgtgag
    67801 cccaggatcc ccaacccaaa tatgtgttga aaaacctgta tgcccaggaa ttcggaaaga
    67861 attgtgcaat ttgtcccggg aatgctatta atgacattgc aaatataacc ggtattactc
    67921 cggcttgatt taccttaagc ggaatatgag tgctctgtcc cccatacatc cttctgccga
    67981 caacccgctt tgcatactgt accggtattc ttctttcacc ctcctgaata aatattacag
    68041 ccattattat caatatcgca aatatcagga agaaggcgat ctcaataaga ttggttgatt
    68101 ttaccttgta gagttcaaaa attttcagag ctccgccggg cactcttgag attataccgg
    68161 caaaaatcaa aagagatata ccgtttccga tacctttttc atttatctgc tcgcccagcc
    68221 acatcaggaa ggctgttccg gctgtgaggg aaagtgctac caatataaca gatgcagcat
    68281 ctgttctcag aagataccct ctgagtccat aagacaaagc cgccgcctga attagtgcaa
    68341 ggacaaccgt gccgtatctt gtgtactgtg ctatttttct tcttccttct tccccttctt
    68401 tagccagggc ttccaggctc ggaattgcaa ttgtaagaag ctgcattata atagttgagt
    68461 taatataagg tgttatgctc attgcgaata ttgcgaagtt atcaaatgct ccacctgaaa
    68521 tagtgttaaa aaatccgagc aaaccgccgc tctgaataag ccgtgacaga gcttcggtat
    68581 tcataccagg tacaggaatg aagcttccca gtctgaatat tataagcatt acaagtgtaa
    68641 aaagcatttt ttgtctcaga tcaggaatct tccaagcatt tcttaatgtg ttaaacaccc
    68701 taaatcacct ctacctttcc tcctgctgct gcaatctttt ctgcagctgt tttgctgaat
    68761 ttatgtgcct gaaccgtcaa gttttttgta agatcgccat tgccaagtat tttaacaccg
    68821 gcaagcatag cttttaccaa gcctgcttct tttaatacct ccggatttac aattgttccg
    68881 ttatcaaatc tttcaaggtc gctgaggtta acaacagcat attcttttct gaatacattg
    68941 gtgaaaccaa tctttggcag ccttctgtac aaaggcatct gtccgccttc gaagccgacc
    69001 cttacaccgc cgccggacct ggatttctgt cctttttggc ctttgcctgc ggttttgccc
    69061 tgacctgttg ccgtacctct gccaagtctt ttcttagact tcgttgtacc ttctgcaggt
    69121 ttaagctcgt gtaatttcat atttacacct ccttgattac atttccttta cttcaaccaa
    69181 atgtataacc ttctgaatca tacctcttat ctgaggtgta tcgttatgtt caacttcact
    69241 gttgattttt ttgagtccca gagccttaac tgtagctatc tggtcatctt tcctaccgat
    69301 tgtactcttt atgagtttga ctttaagttt agccaaggta cttccctcct agcctattat
    69361 ctcttcaacc gctttgcctc tcagctttgc tacatcttca accgttttca atgaagcaag
    69421 gccttgaatc gttgcattta ccatgtttct tggattattt gatctcaagg attttgttct
    69481 tatatcctta atgcctgcca gctcaagaac cgcacgagca gggccccctg ctataactcc
    69541 ggtaccttct tttgccggca tgagaagcac tctgcctgcg ccggcaactc caataacctc
    69601 atgaggtatt gtagttccga ctatggatac ctttataaga ttcttcttgg catcttcgat
    69661 accctttctt attgcttcag gtatttctac tgattttccg gtgccgactc ccacatagcc
    69721 attcatgtcg ccaaccacta cgatggcgct gaatctgaag ttcctgccgc ctttaacaac
    69781 cttagcaact ctatttatgg caacaacctt ttccttaagg tcaagtttac ttgaatctat
    69841 tggctgcatc tatttccctc ccttatccta gaattccaat ccggcttctc tagctgcgtc
    69901 agccagttcc ttgactctgc catgatatat atatccgcct ctgtcgaaaa caacctgctt
    69961 aatgcctgca tctaatgctt tttttgctac cagcgtgcca acggccttag ctgattcctt
    70021 gtttccgccg tgacctggat tatcctttaa ctctttatcc agtgttgagg ctgatactaa
    70081 agtaacaccc tttgtgtcgt caattatctg tgcatatatg ttgttaaggc ttctgtaaac
    70141 atttaatctg ggcctctctg atgttccgaa aaccttcttt ctcattctta aatgtctttt
    70201 ctgccttgta ctgtttttac tggctttagt tatcaccctg tgtcacccct ttgctattta
    70261 tttcttacct gcttttcctt ctttacgcct aattacttca tcagagtact taatgccttt
    70321 gccgagatac ggctccggct ctcttacagc tctgatattg gcagcatgtg ttccgacctt
    70381 ttccttgtcc gcgcccctca caattatttt gttcggagcc ggtacctctg ttgttatccc
    70441 ttccggatca tccatttcaa caggatgtga gtaacccatc gtaagtgtga gcttcttgcc
    70501 cgacttggca gctctgtatc ctacgccgtt tatttcgagt actttctcaa atcccttcga
    70561 aacaccctca accatattgt tcaaaagcgc tctggtaagt ccgtggagtg ccctgtgcat
    70621 tttctggtcg gttggccttg ttactaataa tttgccgtct tttttctcta tattaatatc
    70681 ttcatgcatc ttttttataa gctgaccttt aggccccttg actgaaaccg tattgtcatc
    70741 agctattttt acctcaactc cctggggaat ctcaataggt aatttcccta tacgtgacat
    70801 gcttgcacct cctctatacg taagatatta ccaaacgtag caaagcactt ctccgccgac
    70861 gccctgaagt ctcgcattct tgtccgtcat tatgcctttg gatgttgaaa tgattgctat
    70921 gcctagccct ccaagtacct ttggtatatc ttcatttttt gcatatactt taagtcccgg
    70981 tttgctaatt ctctttaatc ctgttattac cttctgcttg tttgatccat atttgagact
    71041 gattctgata gaatttgtgc ttccgtctcc gattatttca taacccttta tatatccttc
    71101 atcaagcagg atctgcgcaa tagctttttt catattggat gcaggaatat caacagtttc
    71161 atgcttgact gtgttggcat tcctgattct tgttagcata tcagcaattg gatcagttgt
    71221 aaccattaat cttaacctcc ctttttcaga tgtcatcttt ctgaggtctg agaccagaaa
    71281 tcggaggact gattttccct cctgttctga actccgccat aaacttttat tctaacttta
    71341 tatacaattc ttccgcctgt cttgtctgcc ttcgggcatc cggcttccga cttccgactt
    71401 ccgaaattac cagctggcct tctttacacc tggtatttgc cctttataag ccaattccct
    71461 gaagcagata cggcagatac cgaacttcct caaataagca tgaggccttc cgcatatctt
    71521 gcatctggtg taagctctgg tcttgtactt tggtgttctc tgctgcttta ataccattga
    71581 ttttttagcc acaattttac ctccttcgca aagtttctgt attagcttgc ataaggcatt
    71641 ccaaggagtc tcaagagttc tcttgcttcc tcatcagtct cggctgtggt tacaaaaatt
    71701 atatccatgc ctcttatttt atcaacttta tcatattcta tttcagggaa tatgagctgt
    71761 tccttgattc cgagggagta attgcctctt ccgtcaaatg aattaacgga tacgcccctg
    71821 aagtctctaa ctctcggcag agctatattg aataatttat caaggaattc atacatctga
    71881 ttgccgcgaa gagtaacctt agcgccaaca gacattccct gtctcacttt aaatgccgct
    71941 accgattttt ttgcttttgt tataaccggc ttctgtcctg ttattgttcc aagctcagat
    72001 actgcggagt caaggccttt ggcattgtct ttagtatcac ccaagcccat atttataaca
    72061 accttctcaa gttttggtat ttgcatcaca cttttatatt gaaacttctg catcattgca
    72121 ggagctactt cgttcaggta tttctctttt agtctggcca tttcttatta cctcccttca
    72181 gagactattt atccaatatt tcgccgcagc ttttacagta tctgaccttt gacttgtcat
    72241 ccagtattct gtgtcccagc cttacgcctt ttttgcattt gtcgcaccaa agcataacct
    72301 tggaactgaa tatcggcgat tcctgatgta ttattccgcc ctgctgcata tttttgccgg
    72361 gctttttatg cttaacgctc atgtttacac cctcaacaag taccttgtct tcctgcggta
    72421 aagctataag cactttggcc ttcttgcctt tatccttgcc ggagatcacc accaccgtat
    72481 ctcccttttt aacatgtaat tttttaactt ctgcatttgc cattcaaaac acctccctta
    72541 aagcacttca ggtgcgaggg atattatttt cataaaatcc ttttcacgaa gctccctggc
    72601 tacaggcccg aatatacggg ttcctcttgg ctgtttatct tcttttatga ttactgctgc
    72661 attatcatca aatcttatat agcttccgtc cggacgtctc ttgcccttta cagatcttac
    72721 gataacagct tttacaactt ctcccttttt aacaacaccg cctggtgttg catctttaac
    72781 agtagcaact attacatcgc ctatgtttcc atatcttctg gtggaaccgc ctactacgcg
    72841 aatgcacagc agttccttcg ccccggaatt gtccgctacg ttcaaccgtg tttcctgttg
    72901 aatcatttaa aaccctcctt tcggaatttt acttggcctt ctcaattatt tcaacaagtc
    72961 tccatctctt atctttgctt aatggccttg tttccataat tttaacttta tcgcctatct
    73021 tgcattcgtt gttttcatca tgggccttga acttagttgt tctctttatt gatttgccgt
    73081 aaagaggatg acgaaccaat gtctcaatgc ctacaactat tgttttgtcc atcttgtcac
    73141 ttacaacctt gccgattctt gtcttcctat tatttctatc caatgtctaa acctccttct
    73201 tactactttg cctgtacaga cttaagttca ttttctttaa ggatggtttt tattctagca
    73261 atagatttct tcacatcttt taccctcata gggttctcaa gctgtcctgt agccatttga
    73321 aatctaagat taaaaagctc tccttttaat tcattcaact tctgttccag ttcttttacc
    73381 gacatatcgc gaaacttgtt agctttcatt tgtatcacca accatttcct cagtttcccg
    73441 gcgggttaca aacttgcact ttatgggaag cttattctgc gccagcctaa gagcttctct
    73501 ggctatctct tcctcaacac ccgctacttc aaataatact ctgcccggct ttacaactga
    73561 cacccagtat tcaggagcac ccttaccgga acccatacgt gtttcagcag gtttctgagt
    73621 gaccggttta tccgggaaaa tctttatcca tatctttccg cctctttttg tataccttgt
    73681 cattgcctgt ctggcggctt caatctgatt gtttgtgatc cagcccggct cagtagcctg
    73741 aagcccgaat tcaccatatg ctataaaatt gcctcttgtt gccttgccac tcatgctgcc
    73801 tctcatctgc ttacggtgtt tgactctttt tggtatcaac attatgcttc tcctccttcc
    73861 gcagctgctt cattcagctt agcctgctcg ctcttacgag ttttggcttg tggaagaacc
    73921 tcccccttat atatccagac cttaacgcct atccttccat aggtggtttt tgcttctgcg
    73981 aatccataat ctatatttgc tctcaaagtc tgtagaggaa ttgttccttc acgataccat
    74041 tcgcttcgtg caatttctgc tccgccaaga cggcctgcgc acatagtctt aatacccttg
    74101 gcccctactc tcattgtcct ttgcattgac tgcttcattg cccttctgaa tgatattctc
    74161 ttttccagct gtgccgctat attttctgca acaagctgag catccagttc cggtactttt
    74221 atctctgtaa tattaactgt aacacccttg ttgttagtga gcttttccaa ttgtttctta
    74281 agctcttcaa taccggctcc gccttttccg attaccatac ccggctttgc cacgtgtata
    74341 ttaatcttta tttttccggc agttctttct atttcaactc ttgaaattcc tgaggtgaaa
    74401 agcttggttt tcaagaactc tcttatctta ttatcttcta caagaaaatc tgcaaagctc
    74461 ttatcatctg cataccattt tgtgtcccaa tccttaataa tgccaactct tagtccgtgc
    74521 ggatttacct tttggcccac taaaatccct ccttcttcac tctttttcct taaccgccaa
    74581 tgtaatatgg cttgttcttt ttagtatcct tacacctctg ccgtgtgaag ctgctctaaa
    74641 cctcttaaga gtgggtccct gagtggcata aatttctgag atatacaatt tgtcaacatt
    74701 catttcataa ttattctcag catttgccac cgctgatttt aagagcttct ccagtaacac
    74761 tgatgcggct tttggcgtat gtttcaatat tgcaaaggct tcactgacct ttttacctct
    74821 tatcaaatct ataactatat taacctttct tgaggaaatt ctaatatatt tagctattgc
    74881 cctagcttcc acaggaaata cctccttccg tattatcgct tagtttggca ctctttctat
    74941 tttaaagctg aggacctctc cgtatggtgt ccgtggcccc tgaaagttct ggtgggagca
    75001 aattcaccca gcttgtgtcc taccatatcc tctgtcatgt atacaggaac atgcttcctg
    75061 ccgtcatata cagctatggt atggccgacc atttcaggga atattgttga actccttgac
    75121 caggtcttaa gcacaacctt tttgttggtc ttattcatat ctataatttt ctttaatagc
    75181 ttctcatcaa cataaggtcc tttttttgtt gagcggctca ttgatttgcc tcctttcgag
    75241 tactatttct catttcttct cttaacaata aacttgctcg aagctttctt attgtttctg
    75301 gtcttatatc caagtgtcgg cttgccccaa ggggtaacag gccccggcct tcctattggt
    75361 gacctgcctt caccgcctcc atgaggatgg tctgcaggat tcattacaga gcctcttact
    75421 gtcggtctta tgcccatgtg cctcttcctg ccggcactgc ctattgaaat attttcatga
    75481 tctaggttcc ctacctggcc tattgttgcc ttgcattcga ttctaatcat cctgacttct
    75541 ccggagggaa gccttacctg tgcatattcc ccttcttttg ccataagctg tgcagagttt
    75601 cctgctgctc ttaccagctg cgctcccttg cctgccttaa gttctatgtt gtgtatcact
    75661 gtacctatcg gtatgctttt taaaggtaaa gcatttccga cctttatgtc ggcattttct
    75721 gacgatatta tcgtatctcc cacttttagt ccgttaggag caataatata cctcttctcg
    75781 ccgtcagcat aataaagcag tgctatattg gcagacctgt taggatcata ctcgattgcc
    75841 gcaacctttg ccggtatatc aactttgtcc cttttaaagt caattattct atactgtctc
    75901 ttatgtccgc ccccctgatg ccttactgtt atcttgccgt aattatttct tccagcttta
    75961 ctttttaata tttcaaccag ggatttctcc ggttcggttt ttgtaatttc ttcaaaggtt
    76021 gaaaccgtca tattacgtct agctggagaa gtcggattat actttttaat tgccattttt
    76081 tctcctcctt ttacagtcct tcgaagaatt cgatgccctt gctatctttt gtaagcttta
    76141 tgattgcttt cttgtagccg ggtcttcttc cttcatattt tcccattctc ttcattttgc
    76201 ccaatacttt gactgtgttg actttgtcta cttttacctt gaatatctct tcaacagcat
    76261 tttttatttc agtcttgtta gcattaacat caactataaa ggtgtatttc ttgtctgcca
    76321 tttcgctcat gcttttttcg gttattacag gttttctaac tatatcgtaa gcattcatta
    76381 tgcatacacc tcctcgactt ttttaacagc atcctttgtt attacgaact tgtcatactt
    76441 caaaatgtca tatacattca atgtatttac aaaagtggcc tttacaccgt ctatgttcct
    76501 tgctgattta acaacgtttt cattgttgtc agccaaaaca atgagagtct tggaatcaat
    76561 cttgagattc tcaagcaagc ttacaacctg ctttgtcttt ggtgcatcaa agttcagctc
    76621 ttcaataaca actatgctat tctcattagc ctttgaactt aatgcagact tcattgctat
    76681 tctctttatc ttcttaggta ctgtatacct gtatgatctt ggctttggag caaatactat
    76741 gccacccttc ttccactgcg gggacctaat actcccgtgc cttgccctgc ctgttccctt
    76801 ttgtctccag ggctttattc ctccgcctct tacctcagct ctggttaagg ctgactgagt
    76861 tccttgcctc tggtttgcaa gatacatctt aactacctgc tgcattgctt caacgtttac
    76921 atttacgccg aatatatttt catttagttc gatttctcca acctgtttgc cacttatatt
    76981 gtaaagtgct acattaggca ttttacatcc tcctttctac ctcgaatgct ttatcaagct
    77041 ttcactgtat cctttatcat taccagtgaa cctcttattc ccggaatacc gccttttaca
    77101 aggataatgt tcttttccgg gtcaaccttt actacttcga ggtttaccac tgttgtattt
    77161 tctgcgccca tatgccccgg aagcttcttc ccgggaattg ttcttgaggg actggatgac
    77221 gcacccattg aacctgccca tctgtggtat ttggagccgt gcttcattgg ccctctgtgg
    77281 aaattgtgcc tcttgatggc gcctgcaaat cctttaccct ttgatacgcc gctaatatct
    77341 actctgtcgc cttccttaaa gatgtccacc tttatttcct ggccgacttc gtaagatgct
    77401 gcatcttcaa gtctgaattc cctaagatat ctttttgttg caacctttgc tttggcaaaa
    77461 tgtcctttta atggctttgt gacctttttc tcggctttat catcaaagcc aacctgtatt
    77521 gcttcatatc cttcattttc tacagttttt ttctgtacaa ctatgttggg attcgcagct
    77581 attacagtta cgggaaccgc tttgccttca ttatcaaata tctgagtcat tccaactttt
    77641 aaacccagta ttgctttttt cattaattac acctccgaaa tttacagcgg accgcttgcc
    77701 ggtcatacta aatttgatct ggctcgtggt tcagggtccg tggttcgggg tttttgtccg
    77761 gcttcgaagc ccttctcaat cctcgcaccc ggtacctcgt acctcgcacc tgttttataa
    77821 ctttatttcg atgtcgacac ctgccggcag atcaagtctc ataagcgaat caaccgtctt
    77881 tggtgtcggg ttcagtatgt caatcagtct cttatgagtc ctcatttcga actgctctct
    77941 tgaatccttg tacttgtgca ctgcacgaag tattgtgact atttccttct cagtcggaag
    78001 cggcaccgga cctgatacct ttgcacccgt cctttttgct gtctcgacta ttctttctgc
    78061 tgactggtca atcagtatgt gatcatatgc cttcagtctg attctgattt tttgttttgc
    78121 cattttcaat tccctccttt tcatcgcacg ccttattttg ctggcgtgca accgcatact
    78181 gttatacact tatccgggtg tgttctattt ttttatcagt gtccggcatc ccggacactt
    78241 cattttcacc cgcagcagca taatcagtag agtcgcccga attctagaaa cggacattac
    78301 tctgtaagaa ttcagccgca aatcacttca actgcggctt tccagaatac gcccattaag
    78361 gcttaatctg caacctctta cttcatcgca tgctgaataa gtatcgcaac cttatatatt
    78421 ttataacatt ttaggacaaa ttacaatacc tttaaaacca ttgtttaaat atttttttac
    78481 tgtccatttt tacagcctca aaggaccggg ataatcccag tccttgaagc ctttccaagt
    78541 gctcgtgcct cgcacctggc aacctcgtac ctgcgaatag gcgcagccta ttcgattacc
    78601 gatgcaacag cacctgatcc gact
//
LOCUS       DAOOAB010000005        77621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_370_length_77621_cov_16.073383, whole genome shotgun sequence.
ACCESSION   DAOOAB010000005 DAOOAB010000000
VERSION     DAOOAB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 77621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77621
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_77621_cov_16.073383"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            551..982
                     /locus_tag="PKU88_01935"
     CDS             551..982
                     /locus_tag="PKU88_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPX68080.1"
                     /translation="MKSSFEELKEKLKMTNIYLSHQRLKVLEYLVQNHCHPTVDQIFT
                     DLQKDIPTLSKTTVYNTLRILVKSGLVRVITIEDNETRYDIVTENHGHFKCESCGTIF
                     DFPIDIDSLVTGDLSKFKINDKNVYFKGLCPRCLSNINEIK"
     gene            996..1667
                     /locus_tag="PKU88_01940"
     CDS             996..1667
                     /locus_tag="PKU88_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPX68081.1"
                     /translation="MAKYVCSVCGYAYDEAKGIPEAGITPGTAWVDLPEDWICPLCGA
                     TKPEFEKQGEIASTEVKKPTFVIEPSTDIKEMSALEISALCTNLARGCEKQYKLEEAA
                     LFNELAGYFKKASAPAESPDIDQMIALIEKDLEEGFPNANALAADVKDRGALRALVWS
                     EKVTRILKSLLTRYKKEGDAMLENTGVYVCTICGFIYIGDNLPDVCPVCKVPNWKFEK
                     IEGRS"
     gene            1670..2830
                     /locus_tag="PKU88_01945"
     CDS             1670..2830
                     /locus_tag="PKU88_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPX68082.1"
                     /translation="MPKKYAVRNVRLCTKDCLCLYVCPTGATDTENSIIDVAKCIGCG
                     DCADACPSGAISMVPVEYPTQQSKNETVVNSLNVLLRSKSEQENIAAGLSGKLAIAIE
                     KSNHIMAEDIIREAGYMLPESENARAFLNSLLDKKQSEDFPIEVVKKLLDSFKTNNIK
                     LQEDKKMVKYRCSVCGYIHEGELPEGFTCPRCKQPASVFVKVEEKTNSGNQYVGTKTE
                     KNLMEAFAGESQARNKYTYFANIAQREGYDQLSEIFLKTARNEQEHARIWYEELGNLG
                     KTAENLLHAAEGENYEWTDMYDRFAKDAEEEGFKDLAERFRNVAAIEKAHEERYRALL
                     KNVEMQQVFEKGEEIMWECRVCGHLVIGKKAPEVCPVCKFAQSYFEVRKENY"
     gene            2854..3159
                     /locus_tag="PKU88_01950"
     CDS             2854..3159
                     /locus_tag="PKU88_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPX68083.1"
                     /translation="MPDFGQPFSGLKKEHKISHQELIRSIRFMIAAEYEAIQLYMQLA
                     ESTDNKLAIEVLKDIADEERVHAGEFLRLLKELDPDEEKFYAEGAEEVEEEIEKLKK"
     gene            3990..4190
                     /locus_tag="PKU88_01955"
     CDS             3990..4190
                     /locus_tag="PKU88_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX68084.1"
                     /translation="MKNKRMKIARMEVDMKQEDLAKAVGVTRQTISLIESGEYNPTLN
                     LCIAICKELSKTLNDLFWEESK"
     gene            4187..4681
                     /locus_tag="PKU88_01960"
     CDS             4187..4681
                     /locus_tag="PKU88_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68085.1"
                     /translation="MKKQNSQDERVVAQRRKINSEAYGILMIALLCSMLVQQFLLNAP
                     FEQYAVEFICFFGMSLYLMIRHLTLGLNIYGEGKRAKTIPIINGIVTGIVVTTITGVS
                     NYTQYAEHYKEDGIGYFIAVLAITFISATVFSFVVLYCFHYLNKKKQARIQKQLDEKE
                     QDEL"
     gene            complement(4739..5404)
                     /locus_tag="PKU88_01965"
     CDS             complement(4739..5404)
                     /locus_tag="PKU88_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HPX68086.1"
                     /translation="MIAKMIALDWRAMKYYQIRLLLLPVFVFIFGVYSPLTVIPMSVI
                     LSLSFSLNPFAVEEKGELNNLYLTLPVRRKSIVAGRYILSIIMLLCGIAIGILIMPLA
                     NKLSISKWFIGIEEFIVVISLSYLLYAILNLFMFPMLFRLGYHKGKFWGFYLPMVFFA
                     LLFGAYLAITHLPGNKTLTLNFIVYASENLLMVSGSIVVLATMLLILSFMISLKLYSK
                     RDF"
     gene            complement(5401..6153)
                     /locus_tag="PKU88_01970"
                     /pseudo
     CDS             complement(5401..6153)
                     /locus_tag="PKU88_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902276.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(6128..6525)
                     /locus_tag="PKU88_01975"
                     /pseudo
     CDS             complement(6128..6525)
                     /locus_tag="PKU88_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004053920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6750..6989
                     /locus_tag="PKU88_01980"
     CDS             6750..6989
                     /locus_tag="PKU88_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68087.1"
                     /translation="MKNQKWKADEMEIAINYRAMRIAFVFSIIALLAYCIYEYVALKR
                     LPTIPFVIVCVQEVLFFISKVFITSKMTKESDDEE"
     gene            6979..7173
                     /locus_tag="PKU88_01985"
     CDS             6979..7173
                     /locus_tag="PKU88_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX68088.1"
                     /translation="MKNNIRNLRKDKGLRQEDLAQILGVTRQTIIAIENDKYNPTLEL
                     AMKLARFLESSVEDIFILED"
     gene            7443..7883
                     /locus_tag="PKU88_01990"
     CDS             7443..7883
                     /locus_tag="PKU88_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPX68089.1"
                     /translation="MKVEIKIYAEVIEPYAVIYTNEITAEISRIASFIEDSADNSIIT
                     VTDNERMFVLRPEEVYMLRVENEKTVVYTKVKKYNSNKRLYEFETILGSGFIRISKSV
                     LVNLKYLDCVEPSFGGIMLLVLKNGCKDYISRMYLPAFKKYLGI"
     gene            7897..8331
                     /locus_tag="PKU88_01995"
     CDS             7897..8331
                     /locus_tag="PKU88_01995"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HPX68090.1"
                     /translation="MKKLLVKIEFGVAIGCFGFVSMLFIASAFAGGANAFIGQKTGEE
                     MLRLAACFIIIAIGFSVPSLVYEKENLTNSLKVLIHMAAGTVVYLITAFFAGWIEKSF
                     GAIVVYILIALGAAAIIWAIFMLAFKLQADKINRKIREKHED"
     gene            8363..8500
                     /locus_tag="PKU88_02000"
     CDS             8363..8500
                     /locus_tag="PKU88_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HPX68091.1"
                     /translation="MRSFEWILCPVCQSKTRIRILPATVLKNFPLFYPKRKQKTLVNI
                     K"
     gene            8926..9570
                     /locus_tag="PKU88_02005"
     CDS             8926..9570
                     /locus_tag="PKU88_02005"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPX68092.1"
                     /translation="MAVRIKYDDKEKERLIRMTEYERKCYEKGYKLVAGVDEVGRGPL
                     AGPVVAAAVILGEGVLIPGVNDSKKLSEEKREYLYDEIKSKALCCSIGIVDEKTIDEI
                     NILNAACLAMKKALDGLAERPDYILLDAVTLKDMDIPQKGIIKGDTLSLSIAAASIIA
                     KVERDRMISAYDELYPQYAFSKHKGYGTREHIECIKKYGLLPIHRRSFTKNFQG"
     gene            9576..11270
                     /locus_tag="PKU88_02010"
     CDS             9576..11270
                     /locus_tag="PKU88_02010"
                     /inference="COORDINATES: protein motif:HMM:NF040034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPX68093.1"
                     /translation="MKIDASSFRLIKPELIELLKNLETGDVLKGKVLEALGNSILIRA
                     KSGEIFTAILQEGASIPKDAFVELVIKDIADGKIFAELKGEDKASDPDIKVSDLLKQL
                     NLPVNEKNMEAAKLLLKYKLPLNRETIGNITGLQKSIDNLNNSTGEKMGLMLSGLNIK
                     DTPVDVLNKIVLNWPDDFAAFEAILENDGQKPEANEAAIIKQTTAGSASDSKAAEIPG
                     KAAVITITDSSEALKGAVITDSNETPREAAITGRGETPKEGVGYVSPLSDMAAAESNK
                     GVELIRALAGLGIESESEIKMLAAKVESILSSIKNTDMEAITYLVSKEMEITPRNLGM
                     LIKNIENRDVISQFLDKLQEKIIRDDNPKLKAISESIRKVFLEPRQLEDREEVARQLK
                     DIASLGEKLESYLKTGGDKDPEIREALSNLKDCIDFIRNVNQHNNFLQIPLLINDNTT
                     AAKLYVFKDGKREKRINPEDATVVVALDLMNLGHLESMIRVKGKTVDITFRTENKSIG
                     SMIEKNILMLKEALGEKGYYLSPIRVINLEQPFSLLSLEAMINESGYEKIHFDMRV"
     gene            11272..11553
                     /locus_tag="PKU88_02015"
     CDS             11272..11553
                     /locus_tag="PKU88_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HPX68094.1"
                     /translation="MDKKLKKAAALTYEAGDDAPRVTALGKGEIANRIIETAKKNNVP
                     IFEDSGLVDALLQLDIGGQIPQELYSVVAEVLVFVSKIDKLKGEKYGQQ"
     gene            11540..11920
                     /locus_tag="PKU88_02020"
     CDS             11540..11920
                     /locus_tag="PKU88_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPX68095.1"
                     /translation="MGSNKILGAFGENKACEYLEECGYRVLERNFACRTGEIDIIALE
                     GEIIAFIEVKTRSSERYGLPAEAISRNKQNKLVTAALYYMQRNGYQNRMCRFDVMEII
                     IDGEDNYRINLIKDAFQYSGRYGY"
     gene            11939..12508
                     /locus_tag="PKU88_02025"
     CDS             11939..12508
                     /locus_tag="PKU88_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX68096.1"
                     /translation="MLLSNSLAISHEIIKKAVKQGDIVVDATMGNGNDTLLLAGIVGV
                     EGKVYSFDIQDTALINTAKKLREAGVSDIVELICDGHENIDAYVPKGIKAVMFNLGYL
                     PKGDHSIGTKACTTIEALKKSMEMLTDGGIITVVIYYGGDSGFEEKEAVMEYIRTIDC
                     RKYSVLVCDFVNQINCPPIAVCIEKIGRQ"
     gene            13167..13937
                     /gene="pdaB"
                     /locus_tag="PKU88_02030"
     CDS             13167..13937
                     /gene="pdaB"
                     /locus_tag="PKU88_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family sporulation
                     protein PdaB"
                     /protein_id="HPX68097.1"
                     /translation="MNFRFAKNKYILNIMLVVIVVSVSLIYNYAGYKQILNVFRNNNR
                     ELPIYCVDTPEKKVAISFDAAWGADYTESLLKILKNYDVKTTFFLVGFWVDKYPEMVK
                     RIDEEGHEIGNHSSRHPHMSQLSKEQIIDELAKTSEKIEAITHKKVTLFRPPFGDYNN
                     RVIETSRAMGIQVIQWDVDSLDYKDYGADAIVKRVLSKVRNGSIVLFHNNAAYTADAL
                     PIILENLQKEGYKIVPVSELIYKENYYIDHTGMQKLLR"
     gene            14016..15173
                     /locus_tag="PKU88_02035"
     CDS             14016..15173
                     /locus_tag="PKU88_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPX68098.1"
                     /translation="MSKIELVAPCNFAVEAILSREIKSLGYEVTKVEDGRVSFMADEE
                     GICRSNLWLRTAERILVKIDEFEALSFEELFEKTKRLQWNRWIPKDAEFPVAKASSIK
                     SKLFSTSDIQSIVKKAVVESLKTKYKQQWFEETGERYPIHVFLNRDKASLYLDTSGLS
                     LHKRGYRELSNAAPIKETLAAAMVLLTPWKADRAFIDPLCGSGTIPIEAAMIGLNIAP
                     GRGRSFVSDNWRWIGSKLWNRAKEEAGALERRDDVLNIQGYDIDESALKVARINAKMA
                     GLEDKLHFQKRDISELSSKEKYGFIVTNPPYGERLSDSKSVEGLYKEMGKVFSRLETW
                     SFYIITSHEGFERFFGRRADKKRKLYNGMMKTDLYQFFGPKPPRKVEKIDE"
     gene            15224..15409
                     /locus_tag="PKU88_02040"
     CDS             15224..15409
                     /locus_tag="PKU88_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68099.1"
                     /translation="MMDRRFIKKLRKVLVLEAFFHGASSLSKVLDATESDLADIMKYF
                     DKKGSKKELPVGKPNKK"
     gene            15569..16882
                     /locus_tag="PKU88_02045"
     CDS             15569..16882
                     /locus_tag="PKU88_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPX68100.1"
                     /translation="MNVDSHEGIGVTADPQKVRPGMIYVDLSCRRNRRRIYEAYEKGA
                     YMIFTPYNISDPALPVIKVESPRDTLCMLFEKFFEKQKSNAKLIGVFGESDKSILVGM
                     LQSIFSGKDNSRFDMDIIPITINTNENNLFYLDSMSFDSAILTDRCALGKCCGNKPAA
                     DFVSGLTEVRAVIMNNDEYYEINVSENCKRAGLITYGLNKKAVATASSIDVDEITCFN
                     YCVQRSFLSGSGARIEPFEIPVRLNVLGSHSIYNALAAITCGLYYDLDISDIKESIES
                     YKGSARHFQKIYEDGFTVIDNYCNSAYDYTAAFDSIQILSYENLVLVISVSQDVNMNF
                     HEEKAGLIAEWVRILKCKEVILTSCMDGDSHIGTLPLKSMKLYKRIFRENDISFRYYH
                     LLHHAIEKGLSLVNRNDLLVLLGSEEMDAASRIVYKLLGPMKKKN"
     gene            17067..17735
                     /locus_tag="PKU88_02050"
     CDS             17067..17735
                     /locus_tag="PKU88_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPX68101.1"
                     /translation="MTDKVIESNNVTVYGTVTSKPEFSHEMYGEGFYTVYLEVPRLSD
                     ISDTLPITISERLVQGLNIDVGSTLLVEGQLRSYNKYHEGNNKLILTVFARNLKTMDQ
                     DVKNPNQIYLDGYICKKPVYRTTPFGREITDMLLAVNRPYNKSDYIPCIAWGRNARYS
                     ENLKVGDRIRIWGRIQSRDYQKKVSEDEVITRTAYEVSISKMEVIEGETGEKKDNSEE
                     EPEE"
     gene            17853..18389
                     /locus_tag="PKU88_02055"
     CDS             17853..18389
                     /locus_tag="PKU88_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPX68102.1"
                     /translation="MKLEKGLVQVYMGNGKGKTTAALGQGLRSCGRGLRVCMVQFLKG
                     GDTGELHSVEKLYPLFEIFRFERERGFFWTLSEQEKRELKKDIDKGFEFIKNMLRENS
                     CDVLIIDELLGVLENKLLEVNDVLELLKSKPVNMEVIITGRKAPKEIIDAADLVTEMK
                     EIKHYFNSGIPAREGIES"
     gene            18386..19234
                     /locus_tag="PKU88_02060"
     CDS             18386..19234
                     /locus_tag="PKU88_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPX68103.1"
                     /translation="MRKLRYVPILIMIVLGASLGGIAFNAFLIPHKLLSGGVSGVALI
                     LNYIFGLNPGVLIFIFNIPIFLAGYKFMEKEFILLSLVGMTVFSVSIDLFSFLKNVVY
                     IEDTLLSCLYGGVLNGIGMGLVFRNRASQGGVDIVAVIVKKYFSMNLGKTSFLINIIV
                     IGIASLFFGLEPAMYTLVSMYVASTVLDKVQQGFGSSKSVMIITDNEQQVADAILKTL
                     DRGVTYLEGEGAYTGNKKRVIYCIVALKQLAKLKQIVNEIDSSAFMAVSDTAEVLGYG
                     FGNRGI"
     gene            complement(19338..20543)
                     /locus_tag="PKU88_02065"
     CDS             complement(19338..20543)
                     /locus_tag="PKU88_02065"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPX68104.1"
                     /translation="MAIIVQKYGGTSVETIEKINNIAEHIVKRKSEGNDMVIVVSAMG
                     KTTDHLINMAHEISPDPDKRELDVLMATGEQVSMSLLSIALNNLDADAVSFTGQQVGI
                     RTNGSHTKSKIADINEEKIREEHRKGKIVIVAGFQGVNEYNEITTLGRGGSDTTAVAL
                     AAKLGAQCEIYTDVDGIYTIDPRLYPRAKKLDCISYEEMLEMASSGASVMHSRSIELA
                     EKYKVPVLVALSRGDIPGTIIKEMDNTMENTAITGLAVNNDEAIITLNGVPHDIKVIA
                     EIFQSIANKDINIDMISQTFPVNKLVSISFTLPKTDLYQASKVLNTFKKKLFTFTYET
                     CDNITKLSVVGLGMNTQSGVAAKVFNLLAENDIPVSIVTTSEIKISYVISPDDQKKAI
                     EAIAKEFDL"
     gene            complement(20557..21273)
                     /gene="dapD"
                     /locus_tag="PKU88_02070"
     CDS             complement(20557..21273)
                     /gene="dapD"
                     /locus_tag="PKU88_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HPX68105.1"
                     /translation="MNQRLDITNPYEIARYIKESKKVTPVKAYVNGDLKDADMSGIRA
                     YGADNFWILFGDHSDMEKFLSDNKSLIRSFELEYDRRNSAIPLLDTRYVDARIEPGAV
                     IRDKVLIHKNAVIMMGAVINIGAEVGENTMIDMNAVLGARAVIGSNCHIGAGSVIAGV
                     LEPPSADPVIIEDNVLIGANAVVLEGVRIGKGAVVAAGSVVTKNVEPDTVVAGIPARM
                     IKSVKDLNEDKKKLLDDLRG"
     gene            complement(21273..22037)
                     /gene="dapB"
                     /locus_tag="PKU88_02075"
     CDS             complement(21273..22037)
                     /gene="dapB"
                     /locus_tag="PKU88_02075"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPX68106.1"
                     /translation="MIKLMINGCNGKMGQVITRLAQGCDDLSIAAGVTRNPSRLPNSY
                     PVYRSFKEAEVKADVVVDFSNPEMIPETVDYCVKHHTALVVGTTGLSETDLKMLEKAS
                     ESIPVFVSANMSFGIRVLIDLALKAAATLGDEFDIEILEKHHNEKKDAPSGTAMMIAN
                     ELKSRINDGLEFVYDRSKVREKRKPNEIGISAIRGGTIPGEHTVFFAGKDEVIEIKHS
                     ALSRDMFGRGAIRAARYIASKQKGYYTMKTMLEELI"
     gene            complement(22043..22930)
                     /gene="dapA"
                     /locus_tag="PKU88_02080"
     CDS             complement(22043..22930)
                     /gene="dapA"
                     /locus_tag="PKU88_02080"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPX68107.1"
                     /translation="MAIFKGSGVAIVTPFNDNGVDFNKLGELLDWHVENGTDAIIICG
                     TTGEAPTMSDDEKKAAYKFTVDKIAGRIPVIAGTGSNDTHHSIELSKYAESVGCNGVL
                     CVTPYYNKTTQKGLIAHYTAIADSINIPVIIYNVPGRTGINTNADTLKALSVHENIGG
                     IKEASGNISQVVEIGMFCDDNFCMYSGNDDQVVPLLSVGGIGVISVVANIAPKLMHDM
                     VDSFLKGDVKTAMKLQLDIKKLNDALFCEVNPIPIKTAMNLLGMNVGKLRLPLVDMSE
                     GNLNRLKNELINSGFELRG"
     gene            complement(22956..23948)
                     /locus_tag="PKU88_02085"
     CDS             complement(22956..23948)
                     /locus_tag="PKU88_02085"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPX68108.1"
                     /translation="MNKYDVAVVGATGVVGRMFLKVLEERNFPIKNFYAFASVNSAGT
                     KITFSGKEYVVEELSEKSFDRHIDIALFSAGGETSKKYSPVAASKGVVVVDNSSAFRM
                     DPDVPLIVPEVNPHAVKNHSGIISNPNCSTIQAVVALKPLHDKYKIKRIVYSTYQSVS
                     GTGVKGVVDLEEGIKGNAPKIYPHPIAYNCLPHIDVFLDNGYTKEEMKMVDETKKIFD
                     DQSLKITATTVRVPVYYAHSESINAEFENDFDIDELKGLLAGSPGIIVQDDPANCIYP
                     LAINAAGRDEVFVGRIRRDYSVDYGVNLWVVADNIRKGAATNAVQIAELLISGK"
     regulatory      complement(24043..24235)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            24448..25614
                     /locus_tag="PKU88_02090"
     CDS             24448..25614
                     /locus_tag="PKU88_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPX68109.1"
                     /translation="MLHIDDKVQALKDEVISIRRHLHRIPEKGLNEHETTGYIYEYLK
                     DLGINAEISPAGTGVIAYIEGKPGSKAIAFRADIDALSIEEQTGVEYCSKNEGFMHAC
                     GHDGHTAVLLGFARLLVNMGNALDNNVLLVFQPAEEGPGGAEIIVKNGALEQYDASCI
                     FGLHIFPDIEEGKIGCRPGALMAQTGEFDLYIEGKSCHGAMPHKGNDALVAASNVILA
                     LNTIVGRFTDPLEPAVVTVGKMVSGERMNVIAGEALLQGTMRAFNDTTYNTLKDRVIK
                     MADSISGAYGCSCRYEIRDMYPAVHNDERLYKILAEDIGDEDFVYMNPIMLAEDFSYY
                     QKRIPGLFFMLGAGNREKGYIHPLHSNMFNFNDEILITGVQVFYNLLRRCKQFQ"
     gene            25656..26972
                     /locus_tag="PKU88_02095"
     CDS             25656..26972
                     /locus_tag="PKU88_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68110.1"
                     /translation="MGKVIRLLVFTVLVMIMFAVAAYADSIDTGWKGYGLYNLNKLDV
                     TITNESDNITIDGEKVTAVYEYTIKSNADKDIAVNFGIPDNGIVKFSIYDGSKFLNYK
                     TRNTSYLKSTYGAENLQTPDGRWYLFTMVFTPGQTRSISINIEAQMTKEENDTYGLSF
                     FKDRGYSYAIKSEKTWITLNLSDFKPYDIYELEGVKEGQISDNGVATLSFSGDYGNGV
                     FLRYQPIDKMALDSLGKSTQKKPKSIVKAFNEKKYVEALNLCNEYISSPSDSSLNIEQ
                     VKFIKAECMRLSGNYAEYLSTVEQLDISQLYPGRIRFKILYDRLTAYKNTENAEGVNT
                     ILKELIPETQQIYPFLYHWLDRNGYKLLEAEPDAEDEASLNNQVSTENKGFDILGAVI
                     GFFAFVTESRWTYVLLGFIAGFIAGFIIGRITKRRKKSRSVYLFRD"
     gene            27051..27239
                     /locus_tag="PKU88_02100"
     CDS             27051..27239
                     /locus_tag="PKU88_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HPX68111.1"
                     /translation="MKSKKTKKKQIILTPEDKMKYEIANELGLGEKLSQVGWGGLTAE
                     ETGRIGGLITRKKRHLPS"
     gene            27312..28037
                     /locus_tag="PKU88_02105"
     CDS             27312..28037
                     /locus_tag="PKU88_02105"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPX68112.1"
                     /translation="MEIYKKAAELIKKCKPSVVLTGAGISTESGIPDFRSPGSGLWEK
                     IDPMEALSTEVLYKNPKKFYDLGFSIITSMKDSLPNKAHYLVAELEEKGLINLVVTQN
                     IDGLHHKAGSRNVYEVHGTVRTCSCDSCGELYDTGIIEKRIKAGEIPPKCRCRGIIRP
                     DVVLFGDMLPDCFYDSIKKVEEAELLIVIGSSLSVSPVNYLAEICKRLLIINLGETGY
                     DRKSDLIIRESTSSALERIVSFI"
     gene            28034..28777
                     /locus_tag="PKU88_02110"
     CDS             28034..28777
                     /locus_tag="PKU88_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPX68113.1"
                     /translation="MNCYNVLAELYDMFMEEIDYKAWCRYVEDIFKSCGIRPARVLDT
                     ACGTGNITIQMSKSGYNLWGLDISADMLTVAENKARAAKQKITFLNQDMTKMNLKEKF
                     DAVLCMCDGVNYINDEEALKNYFKLVYERLEDIGIFIFDISSYYKIKYIIGNNTFYEE
                     KNNKHYIWNNNFDEKSETVEMDLIFFVPEEGLYRKFSEYHVQKAYRSEYLARLLESTG
                     FEEVSCFDGFSFNKPEEKSERIFFAALKK"
     gene            28805..29695
                     /gene="hslO"
                     /locus_tag="PKU88_02115"
     CDS             28805..29695
                     /gene="hslO"
                     /locus_tag="PKU88_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPX68114.1"
                     /translation="MKDYVVRGASIDDSVRFFSCITTNMAEEGRKIHNCSPVSIAALG
                     RMLTAGSMMGIMLKSDEETITLQINGKGPAGSIVVISDSTGITRGYITNPDVELMHRE
                     NGKLDVGRAVGVDGVFTVIKDMGMKEPYVGQIPIVTGEIGDDISSYFAVSEQIPTAVG
                     LGVLVGIDCSVAAAGGFIIQLMPEADHDMITRLEDRLREIRSVTEMISSGLDAEGMIK
                     SILGDIEYKILEKKQVGYKCSCSRERMERALISIGRNDLEELIQEQGCAEIVCHFCNK
                     KYFFEKNELIEMFEKASSSI"
     gene            29822..29897
                     /locus_tag="PKU88_02120"
     tRNA            29822..29897
                     /locus_tag="PKU88_02120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29855..29857,aa:Val,seq:tac)
     gene            30110..30186
                     /locus_tag="PKU88_02125"
     tRNA            30110..30186
                     /locus_tag="PKU88_02125"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30144..30146,aa:Asp,seq:gtc)
     gene            30198..30273
                     /locus_tag="PKU88_02130"
     tRNA            30198..30273
                     /locus_tag="PKU88_02130"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30231..30233,aa:Phe,seq:gaa)
     gene            30280..30354
                     /locus_tag="PKU88_02135"
     tRNA            30280..30354
                     /locus_tag="PKU88_02135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30312..30314,aa:Gly,seq:gcc)
     gene            30363..30436
                     /locus_tag="PKU88_02140"
     tRNA            30363..30436
                     /locus_tag="PKU88_02140"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30395..30397,aa:Cys,seq:gca)
     gene            30597..31349
                     /locus_tag="PKU88_02145"
     CDS             30597..31349
                     /locus_tag="PKU88_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68115.1"
                     /translation="MKFLKTSFIPDSDVSLAMVDYRISPKIEESLNKIGIECLKTVRC
                     PELYDAVDGHPDMLMFHIGGNRIVLAPNVYEKMAPIIEKKGFAVTKGATWLVRNYPGN
                     IAYNVLRIGKTAFHNIRHTDAEIIKALEKENVKMLNVNQGYTKCSVCILNDNTIITSD
                     EKISEIAEKNGIESFLTKPGGIDLKGLNYGFLGGASGLVSKNKIAFTGSLYTLEDNRK
                     LIDYINKKGFEIVHLSKGRLMDYGSILPLKCR"
     gene            31572..32468
                     /gene="ytxC"
                     /locus_tag="PKU88_02150"
     CDS             31572..32468
                     /gene="ytxC"
                     /locus_tag="PKU88_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HPX68116.1"
                     /translation="MFLVAIGSLDDYEIIKSEIYRQFENAKLEGIDTVYEDYQLGNTL
                     FISCGIKDKLEEFKYILSDILAGVIIGHYESKLIRKIVKENFFYLNGKEQAIVIDNAC
                     KLLGEEKQAQPVGFYKADRKSIIRREILKYLDTEKELNIEGFINFRLSMYVNELNETV
                     ERALEIFVAEREYNEFIKLLRYFVEIQECKVDTVHLLQSGDGSYLLYDDKKNSISSEY
                     FDELRSEILDDTINYDDLLISTLITVSPNRILIHDIDEFKNKELIQTISNVFAERITI
                     CRSCDLCSSLQESSETHSVKLL"
     gene            32551..33921
                     /locus_tag="PKU88_02155"
     CDS             32551..33921
                     /locus_tag="PKU88_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HPX68117.1"
                     /translation="MIIQLLTEEGIRNLQESILELVIKGDGFADVVAKVKEQTDALGR
                     EICKQIFESLDESIYQDPKRKKDWYVERKAESKKIITHLGEIEYKRNYYRSRKGKGYR
                     HLLDELLGIDVHERIDQGVRADMTEHAANISYQKAGELACDVKISRQTVMNGVHKLKE
                     LKVESLGAEHKGIETLYIDADEDHISLQSGKKVMPRLIYIHEGVKKSGSRNELINPFY
                     FASLRKNPDALWQEVYEHVEDTYNTDIIKTVYISGDGAAWIKKGLEYFPKAKFVLDRY
                     HMNKYVIKATANNREYRSRIWEALNLSDLKALKKVMKELYDETESERKRKEIRESFLY
                     FKNNWDGIETYGKERESIVGCSAEGHNSHILADRMSSRPKGWSMEGAEKMARLRAFKA
                     NGGNVYDFMKEQKKEKKLYDITTRLLKETKKKLKQKKTAEIIGNIEVFKIGRITGLYK
                     ALKELC"
     gene            34151..34747
                     /locus_tag="PKU88_02160"
     CDS             34151..34747
                     /locus_tag="PKU88_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HPX68118.1"
                     /translation="MYEALFLIVVGAIIGWLTNVLAIKMLFRPIRPFRIPLLNITIQG
                     LIPKRRNDIAKSIGNVVEKELVSINEIFDRLVTEENKQEVIAIIKEKLLKVIDYKIPA
                     IIPYSIKSRIIDYFDEQINKDAMSILDSSVDGIISNTAYKVKIGEMVEEKIDELELEN
                     MERLILNLTSKELKYIELLGGLLGGIIGFVQTVIIRLL"
     gene            35078..36988
                     /gene="thrS"
                     /locus_tag="PKU88_02165"
     CDS             35078..36988
                     /gene="thrS"
                     /locus_tag="PKU88_02165"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPX68119.1"
                     /translation="MVKITLKDGSVKEYKKGTTVEEIVASIGSGLLKAALAAKLNGKV
                     ADLRTPVNEDCSLEVLTFDDDDGKWALRHTGSHILAQAVKRLYPDAKLAIGPAIDTGF
                     YYDIDSDKSFSEEDLAAIEKEMENIVKEDLRLERFELPRSEALDFMGVMGESYKVELI
                     NDLPEDAVISFYKQEEFVDLCAGPHAPSTGKVKALKLLSVAGAYWRGNEKNKMLQRIY
                     GTAYTKKSELEEYLKMLEEAKRRDHRKLGKELDLFSLHEEGPGFPFFHPKGMIIRNEL
                     ENFWRNIHRKHGYKEIKTPVILNEDLWHQSGHWDHYKDNMYFTTIDEKTYAVKPMNCP
                     GGILIYKNAMYSYRDLPLRMGELGLVHRHELSGALHGLMRVRNFTQDDAHLYLTPEQI
                     KEEVLGVISLTDYVYKLFGFKYRVELSTRPENSMGSDEDWENATEALRNALEASKLPY
                     KINEGDGAFYGPKIDFHLEDCIGRTWQCGTIQLDFQMPEKFNLTYIGADSEKHRPVMI
                     HRTIFGSIERFIGILIENYAGAFPVWLAPVQVIILPVSEKQRDYAEKLNKQLEDLEIR
                     AEADLRSEKVGYKIREAQLQKIPYMLVVGDKEMESGSVSVRDRKEGDAGVMKFEDFLS
                     KIQDEIKNKTNK"
     gene            37136..38743
                     /locus_tag="PKU88_02170"
     CDS             37136..38743
                     /locus_tag="PKU88_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX68120.1"
                     /translation="MKIGNKTLIVLSAFAIIIAVMSGTLKDAARGSYDTEKRLIVGRA
                     NDSVTLDPSCTVEMDSFKVTVNILETLVKCENGGNEVVPLLAESWESSEDGLKWVFKL
                     RRGVKFHDGTAFDADAVVLNFNRWMYEDNPYHKGSFNYWNYIFGGFPGLVKSITALNN
                     YSVEIKLNKPFAPFLYAMTMPAFGIASPEAIKKHQGDLSEHPVGTGPFVYRSWERNKS
                     IILERNDKYWNDAAKVERVEFKVIPGNKDRLEELRQGTIHIADYLSPNDIAGIKYDPN
                     LYLYFRPSFNVGYMAMNNEKSPFNNRLVRAAINHAVDKDKLINDVFDNLAKPAATFIP
                     PSLWGYNGSLEIYEYNPEKSRQLLAEAGYPNGFNTTIWVMNASREYFPDPLKTAQFIR
                     ENLRQVSINAEIVVFNWNEYLKKIRNGEHEIALIGWTGDYADPDNFLYTMLASENAKP
                     GLAGNYSFYKSEDVDQLLAQARQTTNMSFRRSLYRRIQETVNYDMPSVPMVHTMPVLA
                     ARLSVKGYTPHMTGIESLEQVSLENER"
     gene            38763..39362
                     /locus_tag="PKU88_02175"
     CDS             38763..39362
                     /locus_tag="PKU88_02175"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HPX68121.1"
                     /translation="MKRNVFRMVLIIILALLLVLVNMPYKKKAETIVFTLPGLPRPIA
                     KVPVVITSAGQSTDTYLINDISNQLMIRSFFMPQAEAEGIEDMNSIVFAVGYSSLGLK
                     LQGISYEDEKARIEKLINMAKDNKLTIITVVLGGEQPIDVRNEEIISLVGAKSDYIIG
                     LKGSSNEELLADLAENGNIPLTLVRKVKDISVPFASAFR"
     gene            39390..40805
                     /locus_tag="PKU88_02180"
     CDS             39390..40805
                     /locus_tag="PKU88_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX68122.1"
                     /translation="MTASQRKINENILLKTIAAGGVLLIITGMLFPVFFQNTWFNTIM
                     KIREAITTGDSGHLILASASLNSLCSIQSTLLYMGSILLIFNTKLSSRLKSIDVFLVS
                     LVSIVFLHWMDSLIFEIPWEPVSTLLALIISLFLFEKLFVETYSFIQVFIVSVQVFFA
                     FQWLNIMPYFSSYRFGRSDVPYSVKISGIYLNADTVLNFAGFAFFIPFIISAFITATL
                     FISYSRNIHMMRENHIKENEIRDMRAKALENQIYQEVKSLVHDLKTPLVTIRGLNSLM
                     VSENANGKLAEYSERIENSVAKMSDMISSFLYEYSRQKLKASDLISYIRAQLPLEDEK
                     IRFSISIEEDLPDIYINKIRIARAVINVLENAIVVPCKHSYKMINFDVRRAEKGINII
                     IKDNGIGIDESNLTKIWEIGYSTNNTSGLGLPFAKRIIEDNAGTIEVGSQRDFGTTVT
                     IFLPSVDNFEDYGRGEAIEKK"
     gene            40792..41427
                     /locus_tag="PKU88_02185"
     CDS             40792..41427
                     /locus_tag="PKU88_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX68123.1"
                     /translation="MKKNNTPEIVIIDDEPDILYTIKEICLYGGWEAATASNGRQGYE
                     LCRMHKPSLAIVDYHMPDWDGLTTVKRIRQTDNAVSILVLTVDERQETAERFIAAGAT
                     DFAIKPIKSPDLISRIKINLKINEIQKKYILDKQQIYLDKGISPATLSIILDYLKEQG
                     HGLTIDDIANGLNLAYQTVHRYVSYLLEEKRIEIVPIYGQLGRPKNKYKLL"
     gene            41622..42812
                     /locus_tag="PKU88_02190"
     CDS             41622..42812
                     /locus_tag="PKU88_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HPX68124.1"
                     /translation="MKKALALMLAVVMLFSFTACAKPAEPAAAEPAASEAKPEEAKDN
                     WKIAIVTNTVSQNEEEYRSAEFVQKKYGKDKIVHELWPDNFMTEQEQMISVLTKLAAD
                     PDIKAVVINQAVPGTNPAIDKFLEARNKDEVLIVYASPQENAPDVSTRADVILQPDEL
                     SMGSAIPNQALKQGAKTFVHYSFPRHMSIPILAERRELMKAEAAKIGLQFVDATAPDP
                     TSEAGVTGAQQFILEDVPKMVKKYGKDTSFFSTNCSMQVPLIKAAFDAGAIYTQPCCP
                     SPYHGYPSALGLNVPEDIVGGLQDVIDQTTKIVEDAGLSGRFSTWPVPAAMMATIAGA
                     EYAVKWLNGETDGKFDKKVLAECMSDYAGMDISLNPYVENGVTYDNYLLFLVDFLTYG
                     KLPS"
     gene            42956..44554
                     /locus_tag="PKU88_02195"
     CDS             42956..44554
                     /locus_tag="PKU88_02195"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPX68125.1"
                     /translation="MGDKLLTIKNISKEFYGNQVLKDINIEIKKGEIHALIGENGAGK
                     STLMNILFGMPVIHETGGYQGSIELDGKEVMFNSPHEAMFHGIGMVHQEFMLIPGFTI
                     TENIKINRELTNPNLVSKIFGKQYESLDMKRMNSDARKALDIMGMGIEEYTLVAGLPV
                     GHMQFIEIAREIDKTGIKLLVFDEPTAVLTESETQNLLAAIEKLAAQGIGIIFISHRL
                     EEIMQVSHRVTILRDGEHVVTKDTKDTNVYEIAELMVGRKVSLDRSDSDSRRIQDESV
                     LSIKNLEVKMPGEEVKGFDLEVKKGEIIGIGGLAGQGKLGVANGIMGIFPARGQVIFE
                     GRPLELNNPRGAIVAGISFLSEDRKGVGLLLDESIELNTVFTSMQIHKEFLYNLGFIK
                     VMDSQKIRNHTNKMIKDLDIRCTSSLQHAGSLSGGNQQKVCMARALTQKPKLLLVSEP
                     TRGIDIGAKKLILDLLKSLNEEQGMTIVMTSSELAELRSICDRIAIVCDGKVKGILSP
                     EASNAEFGLMMSGVHADSQMGGAE"
     gene            44555..45637
                     /locus_tag="PKU88_02200"
     CDS             44555..45637
                     /locus_tag="PKU88_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68126.1"
                     /translation="MLNNEDKQNNSVKKNLDIPIVKSIGLPTLIIAAFFLLVMITAGV
                     LKQSLPMLISDILRRFGMNGVLVLAMVPAIKSGTGPNFALPLGIICGLLAWTIGIEMG
                     FTGIVLLLITSIIATVFAAATGYLYGRLLNAVKGAEMTIATYTGFSIVALMSLVWLMA
                     PFKSKQMGWMMGKGLRETIKLDGVNAGEILNNFLSFEIIGVKIPTGLLLTFFAFCFLV
                     WLFFRTKTGVAIIAGGSNPEFAAASGIDIDKNRVIANILSTVLGALGIILYSQSYGFF
                     QLYSGPLMMAFPAVAGVLIGGATASKANISHVIIGTFLLQGLLTASMPVANQLFAGTD
                     LSEIMRVILQNGIILYALTQVKGGGK"
     gene            45637..46947
                     /locus_tag="PKU88_02205"
     CDS             45637..46947
                     /locus_tag="PKU88_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68127.1"
                     /translation="MKKAKNMLGKQMPYLTDNIVPIIFIAFTLLGFALTDNVPINYFL
                     SELSSRFYRNAFIVLSLIIPVIAGLGLNFGIVIGAMSGQLAIIIVRYLSIEYLHLSGV
                     WELFFCIALALPIAAFIGFLTGRLYNKTRGQEMIASLIVGFFANGVYQFILLFATGVI
                     IPVRATHPMIKPDGVGVRMTVDMGELHYSLQNIMKTPFMWTVLVVSAVLLILVVALYL
                     TGRKRPDKGNSVIFIIKVLLLAATIAYSAYAIATNNNITKVKDVPTVTIIFIGLLCLF
                     TELIMKTKLGQDFRSVGQSQHIAQSNGINVDKTRIIAVIMSTVMAAWGQIFYLQDMGT
                     LNVYGAHTQIGMFSVAALLVGGASVRKATVSQALIGTILFNSMFIMSPEIGKAVFGNA
                     LLGEYFRTFMVYGIIGMALGIYVWNDNRKARITLEPIELNTPDN"
     gene            47207..48616
                     /gene="pepV"
                     /locus_tag="PKU88_02210"
     CDS             47207..48616
                     /gene="pepV"
                     /locus_tag="PKU88_02210"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HPX68128.1"
                     /translation="MKLEKIVESMKDDIIKAAQELVRIRSIEGNPEPGAPFGKEVRIC
                     LDRSLDICSKLGFKTENFDGFAGHAEYGEGDEIVGILVHVDIVPEGSGWSQNPYGGDV
                     VNDKIYGRGTIDDKGPAAAAIYALKAVADSGVKFNRRVRIIIGCDEESGRWGCMKHYF
                     KYAEAPMCGFSPDADFPIINSEMGILIFNLEKEFEPNEDSRCGGLRIKRIQGGNRVNM
                     VPDYCECELEMKKDFTDRVIKTVEYMKNEQNMKLEMEIRDDKCIINSYGISAHGATPG
                     KGVNAISQLISCICKLPLCVSEQTEYVNFLNSSIGMEIDGKSFGVAMNDEVSGNLVFN
                     LGTIDMSDKKGSVGINIRYPVTKTGKEVYDIIRRKSSEAGIKVIEGNGQDPLYVPADN
                     FMIKILQKVYENVTGQKADLISIGGGTYARAIKNAVAFGPLFPGKEETAHQKDEYIEV
                     DDLMKCTKMFAEAIYELVK"
     misc_feature    48699..48810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            48827..49342
                     /gene="infC"
                     /locus_tag="PKU88_02215"
     CDS             48827..49342
                     /gene="infC"
                     /locus_tag="PKU88_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPX68129.1"
                     /translation="MSSNKDLQINEEIRDRELRIIDSNGDQLGIMSSRQALELAAERQ
                     LDLVKIAPQAKPPVCRIMDYGKFKFEQSKKEKEARRNQKIVNIKEIRFSPTIEEHDLN
                     VKAKNAQKFLQEGDKVKVTIRFRGREVDHVSFGEKALGRFYDIVKDYCVVEKPAKLEG
                     RNMIMILAPKQ"
     gene            49409..49606
                     /gene="rpmI"
                     /locus_tag="PKU88_02220"
     CDS             49409..49606
                     /gene="rpmI"
                     /locus_tag="PKU88_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPX68130.1"
                     /translation="MPKQKTHRASAKRFSFTKTGKVKRACAYKSHILTKKTTKRTRKL
                     RKGAYTTATEAKMIRKLVPYK"
     gene            49628..49987
                     /gene="rplT"
                     /locus_tag="PKU88_02225"
     CDS             49628..49987
                     /gene="rplT"
                     /locus_tag="PKU88_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPX68131.1"
                     /translation="MARVKRAINAHKKRRKILKLAKGYVGARSKKYRIANETVMRALR
                     FAYIGRKLKKRDYRKLWIARINAAARNNGISYSKFINGLKLSGIEINRKMLSELAIND
                     AAAFTKLVDTVKEKLNA"
     gene            50125..51471
                     /locus_tag="PKU88_02230"
     CDS             50125..51471
                     /locus_tag="PKU88_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPX68132.1"
                     /translation="MRKKITMTPTQIIVIGFASMILIGTLLLNMPMASRNGQPVGLIN
                     SLFTATSSVCVTGLVVVDTGTYWTAFGQVVILLLIQIGGLGFMTMSTLLSLLLGRRIT
                     LKERLLIQESLNQFDLEGLVRLTKNIIIATIIIEFIGAIVFTIAFIPDYGLRKGIAFG
                     VFHAVTSFCNAGFDLIGGFRSFTPYVDSCIININTMFLIIIGGLGFSVWMDIYKAIKE
                     RDMRNVTLHSKVVVSTTIILILTGALFIFIMELNNPDTIKKLSLSGKLLASFFQSVSP
                     RTAGFNTLDMAVLTTPTKLMTIILMVIGGSPGSTAGGIKTATAAILFLTVISVIKGKE
                     DTEVFGRHLPQYLVYRAMSVSFISLSLIVIVTMLLSITENADFMTVFFEAASAFGTVG
                     LTLGYSPNLSPFGKLIISMTMFAGRVGPLTIVLALARRAINNRGNVKYPEDRIMVG"
     gene            51489..52157
                     /locus_tag="PKU88_02235"
     CDS             51489..52157
                     /locus_tag="PKU88_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPX68133.1"
                     /translation="MKQFVVIGIGRFGGAVAETLSDLGHEVLVIDTDEEAIQKISDKV
                     THAVTADATDESVLKSLGARNFDVAVVAIGSDIQSSILITLMLKELGVRHVVAKAQNE
                     LHARVLMKIGADKIVFPEREMGERVAHNLISSSILDFIELSEEHSIIEYAVPESWKGK
                     ALRDINMRARYGVTVVAIRNMSNESINISPKADSEIKDGDIMIVIGHNDDLKKLERKT
                     EKEI"
     gene            52154..52963
                     /locus_tag="PKU88_02240"
     CDS             52154..52963
                     /locus_tag="PKU88_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPX68134.1"
                     /translation="MKEIISSSSNNTLKYIKSLQMKKARDEYGKFLIEGEKLFWEALD
                     YNVPISMVLISQSYAESSKHQEYAAALTGRNIPLHYADDRVFKRVCETETPQGIIAVA
                     EKLKYDIDNIISKDKLSLILLHELRDPGNVGTIIRAADACGLDAVAISKGSADLYNGK
                     TIRATMGSLFHIPVIQSVDTAEIIDKLKQRGVVTIGADPHSSINCTELPYQMKSAIII
                     GNESQGISSEIKEMLDINTTIPMPGRAESLNAGIAASILMYELYVRKSLQS"
     gene            53201..54733
                     /locus_tag="PKU88_02245"
     CDS             53201..54733
                     /locus_tag="PKU88_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPX68135.1"
                     /translation="MIYKTNTCALYGIEGHVIEVETDLSNGLPGFDIVGLPDAAVRES
                     RERVRAAIKNSGFLFPQKRITVNLAPADIRKEGSVFDLAIASGILRASMYITNEALEG
                     FAFLGELSLNGSVKGIKGVLPMCLSLKEKGFYKLILARDNADEAALVNGMEIYPINSI
                     TDMAEFLNGNRFITPHKVDLEAILRNDTAETYDFSEVRGQNAAKRALEIAAAGAHNIL
                     MLGPPGSGKTMLARRLPSILPDMSFEESLEVTKIYSIAGLLKDKLYLVTRRPFRAPHH
                     TMSAGSLIGGGRVPHPGEVSLAHYGVLFLDEMPEFQKNVLEVLRQPLEDECVTISRVG
                     GTITYPAKIMLICSINPCPCGYYGDESGRCNCSPNHIKGYLGRISGPLMDRIDLHIEV
                     PSVNFNDLSCSPKEESSAAMKKRINRARQLQLERYENEGIFFNSQLKPKHMTAYCSIG
                     KKEKELLRNAFDRFNLSARAYNRILKVARTIADLEESESIGIDHIAEAIQYRSLDRKY
                     IL"
     gene            54757..55188
                     /locus_tag="PKU88_02250"
     CDS             54757..55188
                     /locus_tag="PKU88_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPX68136.1"
                     /translation="MSTNERDICSVIEKLPVYGKLLIKLYKSADIRKGQKLLLSAGIA
                     YSVSPIELIPGIIPVAGQLDNLIVMLRCLRKVLRSSDEKARERFLKESGMTIEEIDED
                     IKISVDTLKSIGRGTVRIVTNTGKFAGYSVMRHIKEYKNKR"
     gene            55361..56449
                     /gene="dprA"
                     /locus_tag="PKU88_02255"
     CDS             55361..56449
                     /gene="dprA"
                     /locus_tag="PKU88_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPX68137.1"
                     /translation="MKDKKYLMWLSSIPGVGSKTCLRLVKHFSSAENVFHCSFSELMN
                     SGIVREKIAESIKADRNTEKVNEYFRIVKDNDIKVYTIFENEYPENLKHIYDPPPVLY
                     VKGELTAGDSIAISIVGSRKASDYGLKTAHRIALRLAESGITIVSGMAMGIDSAAHRG
                     AIAANGRTIAVFGCGLKYVYPAANFRLSREIEKRGALISEYPFDTEAYPSQFPARNRI
                     ISGMSLGVIVVEAGEKSGSLITADFALEQGREVFAVPGNITSTNSKGTNELIKNGAKL
                     VSRIEDILEEFNIEPICREKSNTFDNRSCEISAEERLILELLHSCGGDRDEIAAASGL
                     QTGKAMAALTMLEVKGLIRQTGGNYHLV"
     gene            56506..58596
                     /gene="topA"
                     /locus_tag="PKU88_02260"
     CDS             56506..58596
                     /gene="topA"
                     /locus_tag="PKU88_02260"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPX68138.1"
                     /translation="MKTSLIIVESPAKVKTIKKFLSSSYKVEASMGHIRDLPKSQLGI
                     DIENNFEPKYITIRGKGEITDKLRKEAKNCDKVYLATDPDREGEAISWHLANILNLNT
                     REVSRIEFNEITKSAVTKALKNPRKLNFNLIDAQQARRVLDRLVGYKISPLLWKKVKK
                     GLSAGRVQSVAVRLICDREDEIRTFVPREYWTLAAKLNDPDTRKSFDAKFFGTENEKI
                     EISSESEMEKILEALKGAKYTVKRIKTGEKRRNSPNPFITSTLQQEAYRKLGFTAKRT
                     MMVAQQLYEGVDIKGEGTLGLVTYIRTDSVRISEEAKKDAAGYIMNKYGKSFLGGEIK
                     QNKSGKKVQDAHEAIRPTSVYREPESIKDSLDKEQYKLYKLIWERYVASQMQAAVFDT
                     VTADIEANGYIFKAYGSILKFPGFMSVYKESSDEKEEEEISLPRLTEGQLLKLKKLEH
                     KQHFTEPPLRYTEATLVKILEEKGIGRPSTYAPIISTILARGYVIRDKKFLVPTDLGE
                     KVTGIMKEYFSDIVDAEFTANMEGKLDNIEEGEKDWVHVIREFYGPFEKTLKSAEEKV
                     GKIKIQDEVSDVVCEFCGRNMVYKQGRFGKFLACPGFPNCRNTKAIVESTGINCPNCT
                     GELIARRTKKGRKFYGCSKYPECSFVIWDEPVAEKCPECGSLIVKKYNKKGFEYKCIN
                     EACGYKRSDNNEVK"
     gene            58599..59909
                     /gene="trmFO"
                     /locus_tag="PKU88_02265"
     CDS             58599..59909
                     /gene="trmFO"
                     /locus_tag="PKU88_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPX68139.1"
                     /translation="MKEKAVVIGAGLAGSEAAWQLAKRGIKVRLYEMRPVASTPAHHT
                     DKFGELVCSNSLRSNQLENAVGLLKEEMRRLGSLIMECADSKALPAGGALAVDREGFS
                     QEITDRITGNSNVEIIREEVTCIPENEYVIVATGPLTSEKLSFSIGRMMDSDYLYFYD
                     AAAPIITYESINKDKVFMASRYEKGDSDYINCPMSEEEYVNFHRELVNAETAPLKSFE
                     KEVVFEGCMPVETMAKRGLQTLLFGPLKPVGLTNPRSGKQPYAVVQLRQDNKEGTLYN
                     IVGFQTHLKWGEQKRVFGMIPGLENAEFVRYGVMHRNTFINSPKVMRPTLQFHNNDKL
                     FFAGQMTGVEGYVESASSGLVAGINMARIIDGREPVVFPETTAHGALCRYITDRTIKA
                     FQPMNVNFGIFPQPEYRIKNKKEKSSYYAERALKDLEKFIRERL"
     gene            60012..60542
                     /gene="hslV"
                     /locus_tag="PKU88_02270"
     CDS             60012..60542
                     /gene="hslV"
                     /locus_tag="PKU88_02270"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPX68140.1"
                     /translation="MFHGTTIAAVKRNDKGAIAGDGQVTFSQSTIIKSTAKKVRTLFD
                     GKVLVGFAGSVSDAFTLSEKLEEKLEQHSGNLKRAAVELAKDWRRDKVLRQLEAMLIA
                     MDKETLLVISGSGEVIEPDDGVIAIGSGGNYALAAAKALIQNTDLPSHEVVRKSLEIA
                     ASICVFTNNNIIVEEL"
     gene            60552..61952
                     /gene="hslU"
                     /locus_tag="PKU88_02275"
     CDS             60552..61952
                     /gene="hslU"
                     /locus_tag="PKU88_02275"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPX68141.1"
                     /translation="MNELTPKQIVAELDKYIIGQDKAKKSVAVALRDRYRRNLLDEEM
                     RAEVTPKNILMIGPTGVGKTEIARRIAKLVNAPFVKVEATKFTEVGYVGRDVESIIRD
                     LIEFSVRLVKAEKISKVNEKAFDIAAEKVAEILVPSSNKSSMSKNPFEMIFNYGGSKE
                     TESDEETKKKEESLAANRYYVMQNVKNGDLNNENIEIEIEDTSGHSIEMLSGPGMDQM
                     SINLQDMFGGILPKKTKRRRVTVKEALKLLTQQEAQKLIDMDEVIEEAVKKAEERGII
                     FIDEIDKIAGRGNIGGPDVSREGVQRDILPIVEGSTVITKYGPVKTDYMLFIAAGAFH
                     VAKVSDLIPELQGRFPVRVELDSLSKNNFVEILTKPNNALIKQYKALLRTEGININFE
                     EDAVEEIASIAAFMNEQYENIGARRLYTVMERLFEDISYEAPDMKSGELNITKEYVQK
                     ALIHDIKRKDVSNYII"
     gene            61967..62749
                     /gene="codY"
                     /locus_tag="PKU88_02280"
     CDS             61967..62749
                     /gene="codY"
                     /locus_tag="PKU88_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HPX68142.1"
                     /translation="MSSTLLEKMRKVNKVLQTSGTQSVSFHDLSKILSEVLNANVYIL
                     SRKGRILGYSLGTSEECEFLKNELLTEKKFPDEYNKSLLSYHETRSNLINKDKLCVID
                     NRERCDMGTNYSTIVPVNGSGERLGTMMLAKYNQEFTDEDLVLAEYGATVVGLEILRS
                     KHDEIEEEARKKAVVQMAIGTLSYSELEAIEHIFKELDGNEGLLIASKIADKVGITRS
                     VIVNALRKFESAGVIESRSLGMKGTHIRILNDKLLEELKKVR"
     gene            63074..63478
                     /gene="flgB"
                     /locus_tag="PKU88_02285"
     CDS             63074..63478
                     /gene="flgB"
                     /locus_tag="PKU88_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPX68143.1"
                     /translation="MIDKISNKTLMLEKALDAAWMRNEAISNNIANVNTPGFKKSYVR
                     FEEYMADAQEKFQISGAKKDPRFLPIGKDRVISANPEIVRENSTSMRRDGNNVDIDVE
                     MAELAKNTIKYNALIAQLSKEFAKIKFAINGR"
     gene            63484..63927
                     /gene="flgC"
                     /locus_tag="PKU88_02290"
     CDS             63484..63927
                     /gene="flgC"
                     /locus_tag="PKU88_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPX68144.1"
                     /translation="MAFLNSINISASGLTAEKLRMDVISRNIANVNTTRTAEGTPYRR
                     QVVVFREGEGSTPFSEYLSDASRRLIGAGVKVTGIKEDKTPFKSVYDPGHPDADENGY
                     VKMPNVDIMTEMVNMISASRAYEANVTAINSTKSMALKALEIGRR"
     gene            63964..64260
                     /gene="fliE"
                     /locus_tag="PKU88_02295"
     CDS             63964..64260
                     /gene="fliE"
                     /locus_tag="PKU88_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPX68145.1"
                     /translation="MKINFPVGQFGIDSNLNNNAASRKEALSFTDFLNNAIKDADKLQ
                     TESEQLNQAFAMGLNDNLHQIVIASEKADIALQFTVQIRNKVLEAYQEIMRMPV"
     gene            64290..65885
                     /gene="fliF"
                     /locus_tag="PKU88_02300"
     CDS             64290..65885
                     /gene="fliF"
                     /locus_tag="PKU88_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPX68146.1"
                     /translation="MGEFFNNLRNNTVEFWNGLKKGQKVRIIIAGLLSFVLIVSFITY
                     MSRPQMGILYSDLSQGDAGTIIARLKEMNVNTRIEGTTIYVPVKRIDELRAQLAVEGI
                     FGDDILYPEELQATFYETSQDKNQRYLIAKQNKLKKGLSAIEGVEYADVNLYIPEDQT
                     FIIDQNSSEATASLIIKMKPGYPALDRNQVNGIVQYIARSVKGLKPENISIIDENGRS
                     LVPDTGTPNSIISSQMEMQEAVQDKIEKSITKFLEAPFGINNVKVKAAVKLNYDSVTQ
                     NITTYEAPNTEQNEGIVRNMQDIRKEWVDAGQGGVPGTDSNTDINQYAEVDSSKAQYN
                     EASTIVNYEINEIKEQIIKEMGSIESLSVSVLINTLDSEGNPKQIPQELQDRVGALVK
                     YALQGFNYTASTEENEGIEVVFAPFDMELANKIKADQEAERKAVFWERIIMIGTGVAA
                     LLVFGGVIFIMLRKRTGAYGAGSGYAVDSTGRAMSEAALSGEPIAEIDIEDKNEVKKK
                     IEKFVGLKPETVAQLLKTWVNEE"
     gene            65897..66913
                     /gene="fliG"
                     /locus_tag="PKU88_02305"
     CDS             65897..66913
                     /gene="fliG"
                     /locus_tag="PKU88_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPX68147.1"
                     /translation="MSRGLKGTFNGRQKAAVLLITLGPEKSAAIFKHLKEEDIEQLTL
                     EIANMRTVTPEEKEEVLNEFYQICIAQEYISEGGISYAKDILERALGGQKALDVINRL
                     TASLQVRPFDFVRKADPAQILNFIQGEHPQTIALILAYLKPQQAAAIISSLPQDKQAD
                     VARRVAIMDRTSPEVIKEIERVLERKLSSMVTQDYTIAGGLAAVVDILNSVDRGTEKF
                     IMETLEVQDIDLAEQIRKRMFVFDDIVTLDNRSIQRVTREVENSDWALALKNAGDEVK
                     KAIFSNMSKRLAEMITEDMEYMGPVRLRDVEEAQQKIVNIIRKLEDAGEIVISRGGGD
                     EIIV"
     gene            66906..67661
                     /locus_tag="PKU88_02310"
     CDS             66906..67661
                     /locus_tag="PKU88_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPX68148.1"
                     /translation="MSKVYKSRNITIGFPKHLVHVFRKETIKEEECKTINTDDNNTED
                     EEEYANSIVEDAKQMYLKIIEEANSEARRIMEAAEEEARSLLSNSREEGYNEGYESGY
                     LEGKNEAQSIIDEASEIKEFLDRRRESLYKEAEEQIVDLVLSISKKVLGQELSQNKET
                     LLSLVNQALQKCTFKNKLVLKISPSDFEFVKENKYRICKMVEGISDIDIVSDLSLPKG
                     SCIVETPSGEINSSIEVQFNEIKKIFTYLLRNE"
     gene            67668..68981
                     /gene="fliI"
                     /locus_tag="PKU88_02315"
     CDS             67668..68981
                     /gene="fliI"
                     /locus_tag="PKU88_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HPX68149.1"
                     /translation="MMGIDMEKYNKALLSNELIKYTGRVSQVIGLTIESHGPAVNLGE
                     ICNIYPLKGNTPIKAEVVGFKESIVCLMPLGSMKGVGPGSKIEATGESLSVGVSNDML
                     GRVIDGLGRVIDGKGTINNCTMYPVENSPPNPLSRKRITEILPVGVRAIDGPLTLGKG
                     QRIGIFSGSGVGKSTLMGMVARNTKADINVIGLIGERGREVREFIENDLKEDGLSRSV
                     VVVATSDQPALIRLKAAQLATSVAEYFRDQGKDVMLMMDSLTRFAMAQREVGLAIGEP
                     PVTRGYTPSVFSVMPRLLERSGTSDKGSITGLYTVLVDGDDMNEPITDTVRSILDGHI
                     VLSRSLANKNHYPAIEVLASVSRVMPNIVDDEHKENANEFKNIMSVYKDAEDLINIGA
                     YVKGSSSSIDRSIEKIGAIERFLKQGVDEKTEMEEILNEMRIIVS"
     gene            68991..69434
                     /gene="fliJ"
                     /locus_tag="PKU88_02320"
     CDS             68991..69434
                     /gene="fliJ"
                     /locus_tag="PKU88_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPX68150.1"
                     /translation="MAVYKFRLQKLLDYKTKVEEEKKNKLGSAVKRLEKEKHKMAELK
                     KEFSDMQSLFQEKTSQGMAVNELKILANYIDYYKRGIKAQKVRIKMAEDYLSACREEL
                     IAATREKRMIEKLKEMDYSKYLYEEQKKEEKLVDDLVSFKESNKS"
     gene            69447..70088
                     /locus_tag="PKU88_02325"
     CDS             69447..70088
                     /locus_tag="PKU88_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68151.1"
                     /translation="MNQPSNTNEKKPAAEKSKKADSKRGKTREKKANTLLLLIIVIFL
                     SAIVAFGAIFMFNFAGLRAETSVWISNLPVVGKLLQPVLQKKNPEQIEREVIEREKSN
                     IAIQQKELNNLEKELLSREKEIEKKEKELADKELEISKLHEQLSEKLRSIQEQVQYLE
                     KVDNQKAMQIILNMNDKASAVQILRNMKKEKAASILAQMDPLQAAQILEDLAD"
     gene            70103..71659
                     /locus_tag="PKU88_02330"
     CDS             70103..71659
                     /locus_tag="PKU88_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPX68152.1"
                     /translation="MTFSIGPMPAFNVSKPDNSSNAAFFQGRPENEFSSFREIFRNTL
                     KSGDRYDTKTAAEEDTKMELAGRAEIRHKKSSNRDPEPVNVGDENPAAEKEVRFKDVI
                     ANDVIAEGANVEKAVADDEAVKEAIVKELLMKLEKLNKMKDGENTDAEKQALLEGIKE
                     LLQELAEIRTEISGEEAAGICEMVSELENVPSKLRDVIIAIFEKPAGTKAAVSAEVQN
                     DIIPTEELNSEKNPVKVPTPEKADAKDNLGDESSKMPAPDESQKNDVKTVSSSKPSVP
                     EQKGDKAETDAKEENIKAAMDDKVEKVSVEENPEKVQETGKNNNRKDAAKETHDTINT
                     THNKLDMEGTAVRQEQNIIFSKAETVQNQAEMPKAQPVSKVEIINQIVKKAEVILTGT
                     QSEMKMQLEPENLGKLTLRIAVERGVITAKFTAESYEVKQVIESNFNELRDMLQDKGL
                     DIQNLSVSVGHESKEFNFSKNYETWRDKLKSGAQNMRHDTYGGYQDEIGTTAAINPYS
                     IHTGRFDQRA"
     gene            71671..72327
                     /locus_tag="PKU88_02335"
     CDS             71671..72327
                     /locus_tag="PKU88_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPX68153.1"
                     /translation="MPVSGINGQYTAYEPNKPKEYNSNLDKDAFLKILVTQLRYQNPL
                     EPTKDNEFIGQMAQFSSLEQSQNSNKAIRMNSANNMVNKLIKANYRAEDSTETKELIG
                     LVEKVMLKDNEIYLTMDVLGTKYDVKFDDVREVTELENSVEQIYLMNQTMRSTTAFNL
                     IGKNVKGTYEETEKVNGVERIKTVEIEGTVEKVRKEGTSIYLTVNGKEIYLEDITEVN
                     "
     gene            72349..72738
                     /locus_tag="PKU88_02340"
     CDS             72349..72738
                     /locus_tag="PKU88_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HPX68154.1"
                     /translation="MSVIYNNKPIIPIGKPIQPTKPSDGRQSDKSISFDAVLQQELSR
                     QSEVKFSKHAAERLQMRNIHLTKDDLNKLNEAVNKAAEKGVKETLIIMGNSAFIANVK
                     NKMIITAATEDNLKNNVFTNIDGAVII"
     gene            72817..74202
                     /locus_tag="PKU88_02345"
     CDS             72817..74202
                     /locus_tag="PKU88_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPX68155.1"
                     /translation="MMRSMFSGVSGLRAHQLKMDTIGNNIANVNTVGYKSQRATFQEV
                     FNQTLRGAGSPQAGKGGTNPQQVGLGISLSSIDTFHVRGAVQRTDNLTDLAINGDGFF
                     ILSNSEDGLSRSYTRAGNFSLDDDGNLVSANGFKVLGYMVDPDTNVLRSQLEGVKISK
                     SEMVAAKETTYATFEGNLDNQIAVAPGADADYVFTAGGDENTPDTLTIPADTKYWETT
                     STVYDTLGGKHTLKFTFIRGMEADGAVGGGPSQWQVFIKDQDLGKYYNESGGFAASDT
                     LETITHGLTFKPDGTLDTTSTNGIVELTVPGKNGSGDIEDLKIDFTKLTQFANESNAA
                     MTGSDGYPQGSLDTFSIGPTGEINGIFTNGMSKIIGQIALAAFKNPAGLEKTAENMFQ
                     VTPNSGDPIIGLPGFSGLGSLNSGTLEMSNVDISNEFAEMISTQRGFQANSRIITTSD
                     EMLQELVNLKR"
     gene            74267..74479
                     /locus_tag="PKU88_02350"
     CDS             74267..74479
                     /locus_tag="PKU88_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HPX68156.1"
                     /translation="MIKVTRLNGIVYYLNCEHIETLENTPDTVITLRDGKKLLVSESP
                     EEIIEKIIEYKKRIFDLSSLIIKDNF"
     gene            74496..75287
                     /locus_tag="PKU88_02355"
     CDS             74496..75287
                     /locus_tag="PKU88_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPX68157.1"
                     /translation="MDLASVFGLVGGIAVILITILISGDLIGYVDIPSIICTFGGTIA
                     MTFMAFPIKKLKEGIAALKYVFVYAETDAEYIIKSIIDLANTARKEGLLALEEAAQQL
                     KDDFLQKGILLIVDGTDPELVRNILETELSFIEERHKSNQGVYEFMAAIGPGFGMLGT
                     LIGLINMLANLSDPSTVGPNMAVAIVTTLYGSIIANVFCNPVISKLKIRSSQESLMKE
                     VMLEGMLSIQAGENPRIIEEKLKAFMSPKLRNMLDSAKEPKEGEE"
     gene            75290..76012
                     /locus_tag="PKU88_02360"
     CDS             75290..76012
                     /locus_tag="PKU88_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPX68158.1"
                     /translation="MARKRVQDEPSPGAPAWMSTYGDMVTLLLCFFVLLFSFATLDVQ
                     KFEAIKQSMSGSLGVLDSGMTLSMEPLANAFPADSPTEEAEEFKKLYEQMSEYIKENN
                     LEASITLRLDERGLLVRFMDNVLFDSGKADLTPNASDIINKVAEIIRQNDKIIRIEGH
                     TDNVPINTFRFPSNWELSTTRAVNVVKYLIEENGIYAYRLSASGYSDQHPVDDNNTAE
                     GRQKNRRVDMVILRDDIKDLNP"
     gene            76032..76457
                     /locus_tag="PKU88_02365"
     CDS             76032..76457
                     /locus_tag="PKU88_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPX68159.1"
                     /translation="MKRNIILIAAIVLLLLVIVSGIIIYMFFNRPKVAEEEPIKIDKK
                     LIMLSLGDSFVNNVKDSKKICKLTFKLEVDKEVAELVTYRESEIRDRINALIRSKTEA
                     DLEGKEGQEKLQKEIVEIIKKIINSKKIKNVYFDEFIVQ"
     gene            76474..77469
                     /gene="fliM"
                     /locus_tag="PKU88_02370"
     CDS             76474..77469
                     /gene="fliM"
                     /locus_tag="PKU88_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPX68160.1"
                     /translation="MSEVLSQNEIDALLSALNSGEIDVQEIKEEKEERKVRDYNFKIP
                     NKFAKDHTRTLQIMHENYSRLLQTYLSGYLRALVQIEVISVDQLTYNEFTNSMPNPSV
                     LGIVEFAPLTGSIIVEMTPTISFAMIERILGGAGHAFERVRGFTEIELSLLEKIIGQI
                     INFFKDPWKNVIELRPRLKKIETNPQFAQILSPNETVALITLNTKIGSIEGMIHICIP
                     HLVIEPVIPKLSTKFWFSGITKEITGSDLKIIEKKIQSTLLPIRVVLGDSEITVRDFL
                     ELGAGDVIQLNTSVNDDLKIYVGNLKKFSGKPGLKKNKVSVKITRIEKKGDDYDE"
BASE COUNT        25607 a        13383 c        18225 g        20406 t
ORIGIN      
        1 caaagtggta tccaaaatat gcgcgttttt ggtatctttt atattgacgt ttacatttgc
       61 ttaaaacccc acataaaata cataatatcc cttaattcag agcggcataa taacaaagat
      121 atctctttat caataagtta atttattaat tcaaagtaat attttgataa aatttatgcc
      181 atataatcat tggaatttca acaccaaaat aagttaatac gtggtgacag gcaccgacag
      241 cttaatagaa aaccacgctg accagagcct tgattatgat gaagagttga taaggttctt
      301 agttgagaaa gcaactatat ttgaaaacaa cgtggttgtg aggtttaagt caggaattga
      361 agtagaagta taaacgagac ttggagacag gccttcgatc aggagatttt gttcctgttg
      421 gggaaagcaa gatatttatt taaaatcttg ttgatataag tgtataagag atgtataatg
      481 aatttgtaac ggttacagcc ttataataat aacaaatcta aaattccatt atatcaggga
      541 cggtgattat atgaaatcct cattcgaaga attaaaagaa aaattaaaga tgacgaatat
      601 ttatctttcg catcagcggt tgaaggtttt agaatatctt gtccaaaatc attgccatcc
      661 aactgtggat cagatattta ccgaccttca aaaggatata cctacgctat caaaaacaac
      721 tgtatataat actttgagaa ttttggtgaa atcaggtttg gtaagggtaa ttacaatcga
      781 agataatgaa actagatacg atattgtaac tgaaaatcat ggacatttta aatgtgaatc
      841 ctgtgggact atatttgatt ttcccattga tatagattct ttggtcactg gagatttgag
      901 caaatttaag atcaatgata aaaatgtata ttttaaagga ctttgtccaa gatgcctttc
      961 taatataaat gaaatcaaat gaaggaggta atgatatggc taaatatgta tgttctgtct
     1021 gcggatatgc ctatgacgaa gcaaagggaa ttcctgaagc aggtattaca ccgggtacag
     1081 catgggtgga tttgccggag gattggattt gtccactctg cggtgcaaca aaaccagagt
     1141 ttgagaaaca gggtgagatt gcttctactg aagtgaagaa accaacattt gtaattgagc
     1201 cgtcaacaga tataaaggaa atgtctgcat tagagattag tgctctttgc actaatcttg
     1261 cgcgaggctg cgaaaaacag tataagcttg aagaagcagc cctcttcaat gaattggcgg
     1321 gatattttaa gaaggcatca gcgcctgctg aatctccgga tattgatcag atgattgcat
     1381 taattgaaaa agaccttgag gaaggtttcc cgaacgcaaa cgctttggct gctgatgtca
     1441 aggatagagg ggcgcttcgc gctttagtct ggagtgagaa ggttactcgt attttaaagt
     1501 cgcttttgac tcgttataag aaagagggcg atgcaatgct cgaaaatact ggtgtatatg
     1561 tttgtactat ctgtggcttt atttacatag gtgataatct tccagatgtt tgtcctgttt
     1621 gcaaggtgcc aaattggaaa tttgaaaaga ttgaagggag gtcataagaa tgcctaaaaa
     1681 atatgcggta agaaatgtac gtttatgtac caaagattgc ttgtgcctct atgtctgccc
     1741 taccggagca acagatacgg agaacagcat tattgatgtt gccaaatgca ttggttgtgg
     1801 agattgcgca gatgcctgtc catccggggc gatttcgatg gtgcctgtag aatatccaac
     1861 tcaacaatct aaaaatgaaa cagttgtgaa ttcacttaat gtgctcctac gaagtaagtc
     1921 agaacaggaa aacattgctg ccggactatc tggcaaactg gctatagcta tagaaaaatc
     1981 caatcatatt atggctgaag atattatacg tgaagcagga tatatgcttc cagaaagtga
     2041 aaatgctagg gctttcttaa attcactgct tgataaaaaa caatcagaag attttccgat
     2101 tgaggttgta aaaaaattgt tggatagttt taaaactaat aatatcaaat tacaggagga
     2161 taaaaaaatg gttaaatatc gttgttctgt ttgtggatac attcatgaag gagaattacc
     2221 agaaggattt acatgtccaa gatgtaaaca accagcttca gtatttgtaa aagttgaaga
     2281 gaaaactaac tctggaaatc aatatgttgg aacaaagaca gagaaaaatc ttatggaggc
     2341 ttttgcaggg gaaagccaag ctcgaaacaa atacacttat tttgcaaata ttgctcagag
     2401 agaaggttat gatcagctat cagaaatctt tttgaaaaca gcaagaaacg agcaggaaca
     2461 tgctcgaatc tggtatgaag agcttggtaa tctgggaaaa acagcggaga atcttttaca
     2521 tgcagccgaa ggtgaaaatt atgaatggac agatatgtat gatcgttttg cgaaggatgc
     2581 tgaagaagaa ggttttaaag atttagcaga gagattccgc aatgttgccg ctattgaaaa
     2641 agcacatgaa gaacgttatc gtgcattatt gaagaatgta gagatgcagc aggtatttga
     2701 aaaaggtgag gaaatcatgt gggaatgccg tgtatgcggg catctggtaa ttggtaaaaa
     2761 agctcctgaa gtatgtcctg tatgtaaatt tgctcaaagc tattttgagg taagaaaaga
     2821 aaactattaa attttattgt ggaggtaaag aatatgcctg attttggaca acccttttcc
     2881 ggactaaaga aagaacataa gattagtcac caggaattaa ttagatcaat tcggtttatg
     2941 attgctgcag aatatgaagc gattcagctc tatatgcaat tagctgaatc gaccgataac
     3001 aaattggcaa tcgaggtttt aaaggatatc gctgatgaag aaagagttca tgccggtgag
     3061 tttttgagat tacttaaaga acttgaccct gacgaggaaa aattttacgc tgaaggggcc
     3121 gaagaagtag aagaagaaat agagaaactt aaaaagtagt taacctgaag gtaaaatgca
     3181 ataaggcacc ctacgttgga atttcgtaga gtgccttgct tgctttaaat cgtattcttc
     3241 tatattcacc gtcagtcaaa ttcaaatttg gttgactgca tctatcgaac agacaaaaag
     3301 tgtggactgt atctaccgaa ccaataggtt gaaaatgtgc ggttgttgta attaaaaaca
     3361 tacggtcgag ccacgtggag actatagtat tgataacaag ggtaaatgag tgaagttgtg
     3421 aaaaagcctt atataccaag gcttttggca catatgggtg taaaacctat tgaatgaaaa
     3481 tcgtcaaaat atcgcttcgt gggaacaagt ccaacagaga tattttgata gttaagtggt
     3541 cgggttagat gtcatagctc agtgagtgta caggttagat gtcagcgttg acgagtggtc
     3601 aggttagatg ttttttcgga tatttttgag gttgtgtggt caggattgat aaccgcatgt
     3661 aaaaaatgca gcttatcgtt aataaacatg gatttgttgc gcaggatttg atctttgggt
     3721 tatttaaata atggacagga ataaatacat ggctatagca aggttgcata tttcagcgta
     3781 acagggtatt tatatttttt tagagtgaca taaaccccac aagagtcttt agaattgctc
     3841 ctgtgggttt tttctttttt acatagaaag cagttgccat gaaaatttaa caaatataaa
     3901 cttaatactt aattgcaaac cgaaatgcaa tatataatag acaccatgca ataaatagtg
     3961 tatactatga ctataaaagg aggcatttta tgaaaaacaa gagaatgaaa attgcccgaa
     4021 tggaagttga tatgaaacag gaggacttgg caaaagccgt aggcgttacc cgacaaacta
     4081 tcagtctgat agaatcaggg gaatataacc ccacgctaaa cctttgtata gcgatttgta
     4141 aggaacttag caaaacatta aatgatttat tttgggagga atcgaaatga aaaagcaaaa
     4201 ttcgcaagac gaaagagttg tagcacaacg ccgtaaaata aatagcgaag catatggcat
     4261 tttaatgatt gcgcttctct gttcaatgct cgtacagcag tttctgttga acgctccgtt
     4321 tgaacagtat gccgttgagt ttatatgctt cttcgggatg tccttatact tgatgattcg
     4381 acacctgacc ttgggactca atatatacgg cgaaggaaaa cgtgctaaaa ccatccctat
     4441 tattaatggt atagtaaccg gaatagtagt aacaacgata accggtgttt cgaactatac
     4501 acagtatgca gaacactata aggaagatgg tataggttat tttatcgccg tgttggcaat
     4561 tacttttatc agcgcgactg ttttttcttt tgtagtactc tattgctttc attatctgaa
     4621 caagaaaaaa caggcaagaa ttcagaaaca gttagacgaa aaagaacagg acgaattata
     4681 ggaagtcaaa gcggtgataa gtttaaatac ctgctccgca aggctcaggc tcatttgttt
     4741 aaaagtcgcg tttggaatac agtttaagag aaatcataaa agagaggatt aaaagcatag
     4801 ttgccaaaac aactatgctt ccgcttacca ttaacaaatt ttctgaagcg tatacaatga
     4861 aattaagtgt aagcgtttta ttccccggca agtgtgtaat tgccaagtat gctccaaaaa
     4921 gcaaagcgaa aaaaaccata ggtaaataaa agccccaaaa tttaccttta tgatacccaa
     4981 gcctgaatag cataggaaac ataaaaagat taagaatggc atacaaaaga taactcaatg
     5041 atatgaccac tataaactct tctattccaa taaaccattt tgatatagaa agtttattcg
     5101 caagcggcat aatgagaata cctattgcta taccgcataa cagcattatt atagatagaa
     5161 tatatcttcc cgcgacgatt gattttcttc tcacaggtaa ggttaaataa agattattta
     5221 actctccctt ttcctcaact gcgaaaggat ttaatgagaa acttaatgac aggattacgc
     5281 tcatgggaat tactgtcaac ggagagtaaa ccccgaaaat gaaaacgaaa accggcaaca
     5341 gcagtaatct tatttggtaa tatttcattg cacgccaatc aagggcaatc attttagcaa
     5401 tcataactcc gatcccttcc tgttcatatg aaccaaaata tcatctagtg aaactgtttc
     5461 agtaattacg cccggcggga aacctccgat ttccgcaacc tcaatcatac cctcaaaaac
     5521 gccgatatgc tctcttagtc ctataatact tttacgtttt gactgtggca agtccccgtt
     5581 accgcccctg attagggagt atttatcgat cagttcatgt ttgagcccac tatatacgat
     5641 tcggcctttg tctatataga taatgtaatc tgcgattttt tcaaggtcgc ttgtgatgtg
     5701 ggtagaaaag aatacgcttt tattttcttc aaccatgtat tcacgaagta tataaagcac
     5761 ttcatcacgc ataacagggt caagcccgga agtgggctcg tcaagcacca ggagtttagc
     5821 gttatgggat aaagtaaccg ctagaccgag tttcattttc ataccgcgcg acatggtttt
     5881 gtatttttgt ttgggattaa gagaaaattt attaagatac tgatgaaacg ccgaactgtc
     5941 ccactgccta tagaatggat acagagcctt ttcaatatcg ccgccatctc ttaaagccat
     6001 gccaagcata agcttcatcg ttgtcgtttt acccgcacca ttttagccga taaacccgca
     6061 aataaagcca cttggtacag taaagctaat gttgttcaac agaaagcctt tatacttttt
     6121 tgtgaggtta tttaccataa tcgcgttttt cattatacgt ctcctccagt tcatctaata
     6181 atttatgaag ctccgacatt gtcaaccctg ccattttact ttgtcgaata gcggtgttca
     6241 acgtggcttt tgtctcctta acatattggc ttcttataaa atcctcctca attctttaac
     6301 atagcacccc ttacccggta cggtttgaag gaaaccttca agttccaaat cgttatacgc
     6361 tcgtgtagtt gttatgacgc taacttttaa gtcacgagca agctgacgaa tagagggcag
     6421 caattcatct tcttgcaact cgccagataa aactgaggtc ttaatctgtt cttttatttg
     6481 ctcatatatt ggaacgcttg actggtataa caaaacaata cgcatatctt taacccctat
     6541 tttcaccgtc ctagcattgc agtttacctt tctatttgga tatacctaca cagtacccgc
     6601 tattagctta aataattcat aatgcgtgta ttttcaattt ccgtaacaaa aaacttaaaa
     6661 agtaaaatgt aaaaacatct tgacatcttc tgctctgtga gtataatagg aaatgtaaag
     6721 agttctttac atataggagg attatcaata tgaaaaatca gaaatggaaa gctgatgaaa
     6781 tggaaatcgc cataaattac agagctatgc gtattgcttt tgtattttca atcatagcat
     6841 tattggcata ttgtatttat gaatatgtgg cgttgaaaag actgcctaca attccttttg
     6901 tcattgtatg tgtgcaagaa gtactgttct tcatttcaaa agtattcatt acgagtaaaa
     6961 tgacaaagga aagcgatgat gaagaataat atacgaaatt tgcggaagga taaaggcttg
     7021 cggcaagaag atttagcgca aatattggga gttacccgac aaacaattat tgctattgag
     7081 aacgacaagt ataatcctac tttggagctt gctatgaaac ttgcccgatt tcttgaatca
     7141 tctgtcgagg acattttcat tcttgaagat tagtcaaggt aattgtcaac aaataggcgg
     7201 tgatgaaatg gggcatatct taaggcggat acggaaactt tgagagaaaa tccctggtat
     7261 gcaaagtttt atagaatgtg gctgttacca taaagacgca gaaaaaagca acttaatcaa
     7321 tgggtgacaa ctgagtattt gcatgttacc tcttaaaatc agccacttac ctgaaatccc
     7381 cggacaaaca cgcgcaacta ccctataatg tacatgcaag cagaaagcga ggtgagatac
     7441 gagtgaaagt agaaattaaa atatacgcag aagtcataga gccatacgct gttatatata
     7501 ccaatgaaat aacagcggaa atcagccgca tagcctcatt cattgaagat agcgcagaca
     7561 acagcataat taccgttacc gacaacgagc gcatgtttgt cttacgcccg gaagaagttt
     7621 atatgttacg ggtagagaac gaaaagaccg ttgtatatac taaagtcaaa aaatacaaca
     7681 gcaataaacg cttatatgaa tttgaaacaa tactcggcag cggctttatc agaatatcta
     7741 aatccgtatt ggtcaatctg aaatatcttg attgcgttga gccgtccttt ggaggaatta
     7801 tgctgcttgt tctgaaaaac gggtgcaagg actatatctc acgaatgtat ctgcctgctt
     7861 tcaaaaaata tcttggcata tagggaggtg tgttttatga aaaaactgct tgtaaaaatt
     7921 gaatttggag ttgcgatagg gtgctttggg tttgtgtcca tgctgtttat cgcttccgct
     7981 ttcgcgggcg gcgcaaacgc tttcatcggt caaaagacag gcgaagaaat gttgcggctt
     8041 gctgcatgtt ttattattat agcgattgga ttttccgtcc cctctttggt ttacgaaaag
     8101 gaaaacctta cgaacagctt aaaagtgctt attcatatgg cagcaggaac ggttgtatat
     8161 cttattaccg ctttctttgc tgggtggatt gaaaagagtt ttggagcgat agtagtttac
     8221 attttgatag cattaggagc agcggcaatc atttgggcaa ttttcatgct tgcttttaag
     8281 ctgcaagctg ataaaatcaa cagaaaaatt agagagaaac atgaagatta aaagtgataa
     8341 gtcttaggca agtgttggtg agatcagaag ttttgagtgg atattgtgtc ctgtttgtca
     8401 aagcaaaacg cggattagaa tactccccgc tacggtgctt aaaaacttcc cactattcta
     8461 tccgaagcgt aaacagaaaa cgctagtcaa tatcaaataa ccgaatatgt cagttatcaa
     8521 agaggcagac gcacaggcgc agagccgata acacacagat tagaaacgcg gttatcggcg
     8581 gctgctgata caaatccagg atatcttcca tacaggccac aaaagcaaat tcggggctat
     8641 caaaaatctc aaatttttgt tttttctttc gataccataa attttttagt tggctgttac
     8701 aatgtttttg cagactgttt ttggcaatgg atcatcattt ggcgaattga atggtgcttc
     8761 ctaaaatact taatatcaat gaaagttcaa tttcagaata aaataactca aactcctgca
     8821 tcgccacaac aatatgctat aataagcatg tacatcaaaa tgtatatgct tatattttta
     8881 tgcagatata tccgataatt ctatatatac gaatgaggtg taaaaatggc tgtaaggata
     8941 aagtatgacg ataaagaaaa ggaacggctt atccggatga cggaatatga gcggaagtgc
     9001 tatgagaagg gctataagct ggttgcaggg gtagatgagg tgggaagagg cccactggcg
     9061 gggcctgtag ttgcagctgc agtcattctt ggagagggag tgctcatacc cggggtcaat
     9121 gactccaaga agctgtcgga ggagaaaagg gaatatcttt atgatgaaat aaagtccaaa
     9181 gccctgtgct gcagcattgg aatagttgat gaaaaaacca tagatgaaat aaacattctg
     9241 aatgctgcct gccttgctat gaagaaagcc ttggatgggt tggcagaaag gcctgattac
     9301 atacttctgg atgctgttac cttgaaggat atggacatac cccagaaggg aatcataaag
     9361 ggagataccc tgagcctttc cattgctgcc gcatcaatta tagccaaggt ggaaagggac
     9421 aggatgatat ccgcctatga tgagttgtac cctcaatacg cattcagtaa acacaagggt
     9481 tacggaacaa gggagcatat tgagtgcatt aagaaatacg ggcttctgcc catccacagg
     9541 agatccttta ccaagaattt tcaggggtga ttataatgaa gatagatgct tcaagcttta
     9601 gattaataaa accggagctt attgaactcc ttaaaaacct ggagacagga gatgtactga
     9661 agggcaaggt gctggaagct ctgggaaaca gcatactgat aagggcgaaa agcggagaga
     9721 tatttactgc cattcttcag gaaggggcaa gtatacccaa ggatgctttt gtcgaattgg
     9781 ttataaagga tatagctgac gggaagatat ttgcggagct gaaaggggag gataaggcct
     9841 ccgatccgga tataaaggtt tccgatctct taaagcagct caatctgccg gttaatgaga
     9901 aaaacatgga ggctgcaaag ctcctgctaa agtataagct tcctctgaac agggaaacaa
     9961 tcggtaatat cacagggctg caaaaaagca ttgataatct taataacagc accggagaga
    10021 aaatggggct gatgctatcg ggattgaata taaaggatac gcccgttgat gtattgaata
    10081 agatagtact caactggccg gatgattttg cggcttttga agccatactg gaaaatgacg
    10141 gtcagaaacc ggaggcaaat gaggcagcta ttattaagca aacaactgca ggatcggcta
    10201 gtgacagcaa ggcagcagaa attcccggaa aggctgcagt tataacaata acagacagca
    10261 gtgaagctct caagggggct gtaataacag acagcaatga gactcccaga gaggctgcaa
    10321 taacaggcag aggtgagact cccaaggagg gtgtcggtta tgtgagccct ttgagcgata
    10381 tggctgcagc ggaaagcaat aaaggagttg aactgataag agcattggcc ggactgggaa
    10441 ttgaatctga aagcgaaata aaaatgcttg cggcaaaagt cgaaagcata ctatcttcaa
    10501 taaaaaatac cgatatggaa gccattactt atctggtttc gaaggaaatg gagataacgc
    10561 ccaggaatct gggcatgctt ataaaaaaca tcgagaacag ggatgtgata tctcagttcc
    10621 ttgacaagct gcaggaaaag attattcgag acgataatcc aaagcttaaa gccataagtg
    10681 aatccataag gaaggtattt ctggagccca ggcaattaga ggacagagaa gaagttgccc
    10741 ggcagcttaa ggatattgca agcctgggag aaaagctgga aagctatctg aaaaccggcg
    10801 gggataaaga ccctgaaata agggaggcat tgtcaaacct caaggattgt attgatttta
    10861 ttcgcaatgt taatcagcat aataattttc ttcagattcc tttactgata aatgacaata
    10921 caaccgcagc aaagctgtat gtatttaaag acggcaaaag ggaaaagagg ataaaccctg
    10981 aagacgcaac tgttgttgta gccctggacc tgatgaacct ggggcatttg gagagcatga
    11041 taagggtaaa gggcaaaacg gttgatataa ccttcaggac agaaaataag agcataggaa
    11101 gcatgataga aaagaatata ctaatgctga aagaagccct gggggaaaaa ggctattatc
    11161 tcagcccaat tagggtaata aatctggagc agcccttcag cctcctttct cttgaagcaa
    11221 tgataaatga aagcggatat gaaaaaattc attttgatat gagggtatag tatggataag
    11281 aagctgaaaa aagcggcggc tctcacctat gaggcagggg atgatgcccc aagggttacg
    11341 gcattgggca aaggtgagat agccaaccgt attattgaaa ccgcaaagaa aaacaatgtg
    11401 cccatatttg aagacagcgg cctggtcgat gcactgctgc agctggatat aggggggcag
    11461 atcccgcagg aattatacag cgttgtggct gaggtattgg tatttgtttc gaagatagac
    11521 aagctgaaag gggaaaagta tgggcagcaa taaaatattg ggagccttcg gcgagaacaa
    11581 ggcatgtgaa taccttgaag aatgcggtta cagagtgctg gaaaggaatt tcgcctgcag
    11641 gacaggggag attgatataa ttgctttgga aggtgaaata attgccttta tcgaagtaaa
    11701 aaccagaagc agtgaaaggt acggactgcc cgcggaggca ataagcagaa ataagcagaa
    11761 taagctggtc acggcggccc tttattatat gcagcggaat ggatatcaaa accgcatgtg
    11821 cagatttgat gtaatggaaa ttattattga tggtgaggat aattacagga taaaccttat
    11881 caaggatgcc ttccaatact caggaaggta cggctattaa agaaaggaag cttttcatat
    11941 gctgctcagc aattcactgg ctatttccca tgagataata aaaaaagcgg ttaagcaggg
    12001 tgatatagtt gtagatgcca ccatgggaaa cggaaatgat acactgcttc ttgcaggcat
    12061 tgtgggggtc gaaggaaagg tatattcctt tgacatacaa gacacggcac tgataaacac
    12121 ggcaaaaaaa ctcagggagg ctggagtttc agacatcgtt gagctaatat gtgacggcca
    12181 tgagaatatc gatgcctatg tacccaaagg cataaaagcg gtaatgttca atttgggtta
    12241 tcttccaaag ggggaccata gtatcggcac aaaggcctgc actaccatag aagctctgaa
    12301 aaagtccatg gagatgctaa cagacggcgg tataattacg gtggtcatat attacggagg
    12361 ggacagcggc tttgaagaga aggaggcagt gatggagtat ataaggacaa tcgactgcag
    12421 aaaatactcc gtgcttgtat gtgattttgt gaaccagata aattgtcctc cgattgcagt
    12481 atgcatagag aaaataggca ggcagtaaaa taatagtatg ccgcagccaa atatgtataa
    12541 atatggtaat atagtcatgg ttaaaatagt actagctcag cttcttcaat aattgaaggg
    12601 ttgatataat ataaaagaca agcatagtct tgttgcagca cctcccggcc tggtgtgaaa
    12661 ggagggaaaa gaatcactgt gaaacgcagt tgtaaaagaa cgttgcagta tgtgtttaga
    12721 gtgatgaata cccttacaat ctcaacattg atcaggttgg ttgctattgc attagcagct
    12781 attttgctgg taaactatgc gaaatttact ccgagagaat ttatagagct gatacataca
    12841 atcattactt gataaatgat tgagatagaa aaaaacggac actcatggag gagcctgtgc
    12901 ggctacacgc cattgctgct aataaggcga ggccggtgct gcttttacga gggagcagta
    12961 tgatgaagtg agtgtccgtc attttttgca ctttgatgtt tagactaaac aacagcattg
    13021 aaaaattgta gaaaatctgg ttaatataat gaaatttggc gcgaaatttt aatttcgcgc
    13081 caaattatgc tgttttatgc ttggaaatat tctacttcct gatattttga atagattaat
    13141 actaataata tatcaggagg tgcattatga atttcagatt cgcgaagaac aaatacatct
    13201 tgaatataat gctggtggtt attgttgtca gtgtttcgct gatatacaat tatgcagggt
    13261 acaaacagat tttaaatgta ttcagaaaca ataacaggga gcttcccata tattgtgtgg
    13321 atactccgga aaagaaggtg gcaatatcct tcgatgcggc atggggggcg gattatacag
    13381 aaagcctatt gaaaatactt aaaaattatg atgtaaaaac tacgtttttt ctggttggat
    13441 tctgggtgga taaatatcct gagatggtaa agagaattga tgaggaaggc catgaaatcg
    13501 gcaaccattc atcaagacac ccgcatatgt cacagctctc aaaggagcag ataatagacg
    13561 agcttgcaaa gacctcggaa aagattgaag caattacaca taaaaaggtg actttattcc
    13621 gcccgccttt cggtgattat aacaacaggg ttatagaaac ctcaagagct atggggatac
    13681 aagttataca gtgggatgtg gattctcttg attacaagga ttacggggct gacgcaatag
    13741 tgaagcgggt gctctcaaag gtcaggaacg gatcgatagt gctgtttcac aataatgccg
    13801 cctatacggc tgacgcgctt cccattattc ttgagaacct gcagaaggaa gggtacaaga
    13861 tagtgccggt gtccgagctt atatacaagg agaattatta tatcgatcat acaggtatgc
    13921 agaagctgct gagatgaata ataattgacg gatacagtat aattaatgat atactcttaa
    13981 ttaagtaggt acagatatca caaaggagga tggccgtgtc aaaaattgaa ttggtggcac
    14041 cctgcaattt tgcggtagaa gcgatactgt caagggaaat aaagagcctg ggatatgaag
    14101 taacaaaggt agaggatggc agagtctcat ttatggcgga tgaagaagga atatgcagat
    14161 caaatctgtg gcttcgcacc gctgaaagaa tacttgttaa aatcgatgag tttgaagccc
    14221 tttcctttga ggagcttttt gaaaagacaa agaggctgca gtggaaccgc tggataccca
    14281 aagacgcgga gtttcccgta gccaaggctt catcgataaa gtcaaagctt ttcagcactt
    14341 cggatattca gtcaatagta aaaaaggctg tggtggaaag cctgaaaaca aaatacaagc
    14401 agcagtggtt tgaagaaaca ggagagcgct accccataca tgtattcctt aacagggata
    14461 aggcatcact gtacttggat acaagcggtt tgtccctcca caaaagaggc tacagggaat
    14521 tgtcaaatgc agctcccata aaggagaccc ttgccgctgc catggtgctg cttacaccat
    14581 ggaaagcaga cagggccttt attgatcctt tgtgcggttc cgggacaatt cctatcgaag
    14641 cagccatgat cgggctcaat attgctccgg ggcgaggaag gagcttcgtt tcagacaact
    14701 ggagatggat aggaagcaag ctgtggaata gggcaaagga ggaagccgga gcccttgaaa
    14761 ggcgtgatga tgtcctcaat attcagggat atgacatcga tgagagtgct cttaaggtag
    14821 caagaataaa tgcgaagatg gcaggccttg aggataagct tcattttcag aaaagagata
    14881 taagtgagtt aagcagcaag gaaaagtacg gcttcatagt gacaaatcct ccatacggcg
    14941 agaggctttc cgacagtaaa agcgtggaag ggctgtacaa agaaatggga aaggtatttt
    15001 caaggcttga aacttggtcc ttttatatta tcacctccca tgagggcttt gaaaggtttt
    15061 tcggaagaag ggcggataaa aaaaggaagc tttataacgg aatgatgaag acggatttat
    15121 atcagttttt cggcccgaag cccccaagga aagttgaaaa aattgatgaa tagcccggtc
    15181 aattatcata ctcccttaat ataatgtata aagggaggga gtcatgatgg atagacggtt
    15241 tataaaaaag ctcaggaagg tgcttgtgct ggaagcgttt tttcatggtg cttcatcatt
    15301 gtcaaaggta cttgatgcta cagagagtga tttggcggat ataatgaaat attttgataa
    15361 gaaaggcagc aaaaaggagc ttccggtggg taaaccaaat aaaaaatgag gtaattagct
    15421 ttatacagcc ggggaaccat acataaataa atgactgtat gccaattata taatcacgag
    15481 gagaaaagat atccctcact tgtgtctgca ttttcctacc ctcgtttaaa cattgtacag
    15541 atctaactat agtccgggag ttggtaaaat gaacgtggac agtcatgaag gaataggtgt
    15601 tacagcagat ccgcaaaagg taaggccggg tatgatatat gtcgatttgt catgcagaag
    15661 gaacagaagg aggatatatg aagcctacga aaagggagca tatatgatat tcactcctta
    15721 caatatatca gatcctgcat tgccggtgat aaaggttgag agccccagag atacgctttg
    15781 catgctgttc gaaaaatttt tcgagaaaca gaaaagtaat gcaaagctaa tcggagtatt
    15841 tggcgaaagt gataaaagta tattggttgg gatgctgcag agtatttttt ccgggaaaga
    15901 taacagcagg ttcgatatgg acatcatacc tataactatt aatacaaatg agaacaacct
    15961 tttttatctt gacagtatga gctttgacag tgccatactg actgacaggt gtgccttggg
    16021 taaatgctgc gggaataagc cggcagctga ttttgtctca gggctgacgg aggtcagggc
    16081 agtaataatg aacaatgatg agtattatga aataaatgtc tcagaaaact gcaagagggc
    16141 aggattaatc acttacgggc tgaataaaaa ggctgttgca acagcttcca gtattgatgt
    16201 tgatgagata acctgcttca attattgtgt gcagagaagt tttctgtccg gaagcggcgc
    16261 aagaattgaa cccttcgaga taccggtgcg tttgaatgtc ctgggaagcc acagcatata
    16321 taatgcattg gcggcaatta cctgcggcct ttattatgac ttggacattt cagatattaa
    16381 agagtcaata gaaagctata agggctcagc caggcatttt cagaaaatat atgaggatgg
    16441 ctttactgtt atagataatt actgcaattc agcttatgac tacacggcag cttttgattc
    16501 aattcaaata ttgagttatg agaatcttgt acttgtcatt tctgtttcac aagatgtgaa
    16561 tatgaacttt catgaggaaa aagccggcct gatagccgag tgggtaagga ttctgaagtg
    16621 caaggaggta atcctgacca gttgtatgga tggtgattcc catatcggaa cgcttcctct
    16681 gaaaagtatg aaattatata aaagaatttt cagggaaaat gacatttcat tcagatatta
    16741 tcatttgctg caccatgcta ttgaaaaggg cctgtcgctg gttaacagaa atgatttgct
    16801 ggtgttgctg ggaagcgaag aaatggatgc ggcaagcagg attgtatata agctgctggg
    16861 gcccatgaag aaaaaaaatt agaataataa agaaaaagag caatggaatg ctgtttctga
    16921 tgaggacaca acattccatt ttttatgctt attttgaggc agactctttt ctgaatttaa
    16981 aaataattca tattatatgt ttgtagtgaa tatgtatact gtagattaaa cctgaaatac
    17041 acaagaacag gaaaggagcg gggcttatga cggataaagt catcgagagc aacaatgtta
    17101 ccgtatacgg tacagtaact tccaaaccgg aattcagcca cgaaatgtac ggcgagggtt
    17161 tttatacggt atatctggag gttccgaggc ttagtgatat ttccgatacg cttccgataa
    17221 cgatatcgga aaggcttgtg caagggctca atattgatgt ggggtcaact cttcttgttg
    17281 aaggacagct tagatcgtac aataaatatc atgaagggaa taataaactt atacttactg
    17341 tatttgcaag aaatttgaag actatggacc aggatgtaaa gaatccgaac cagatttatc
    17401 tggacggcta tatatgcaag aaacctgttt acaggactac gccctttgga cgcgagataa
    17461 cggatatgct gctggccgtc aacaggccat ataacaagtc tgattatata ccgtgcattg
    17521 catggggcag aaatgcaaga tattcggaaa atttgaaggt aggagatcgt attaggatat
    17581 ggggcagaat acaaagccgc gattatcaga aaaaagtatc tgaggatgag gtaataacca
    17641 gaacggcata tgaggtgtca atttcaaaaa tggaagtaat tgaaggggag acaggagaga
    17701 agaaagataa ttcggaggaa gaaccggagg aataaaagga aaagcgcagt gacaatgcgc
    17761 ttttttttac ttatttatat ttgaaatatt ttctacagaa gttataataa actatatgga
    17821 tgagcataaa acgaaacgga ggggtcgaag ctatgaagct tgaaaagggg cttgttcagg
    17881 tatatatggg aaacggtaaa ggcaagacta ctgcggcact gggccaggga ttgagatcct
    17941 gcggccgggg gctcagagta tgcatggttc aatttttaaa aggcggagat acgggggaac
    18001 tccatagtgt cgagaagctg tatcctttgt ttgaaatatt ccgttttgaa agggaaagag
    18061 gctttttctg gactctttcc gaacaggaaa agcgcgaact gaagaaggat attgacaaag
    18121 gttttgagtt tataaaaaat atgttacggg aaaattcctg cgatgtccta ataattgatg
    18181 aattgttagg tgtattggaa aacaagcttt tggaagtaaa tgatgtactt gagcttctta
    18241 agtcaaagcc tgtcaatatg gaggttatta ttaccggaag gaaagctccg aaggagataa
    18301 ttgatgcggc ggacctggta accgaaatga aggagatcaa gcattatttc aacagcggga
    18361 ttccggcaag ggaaggcata gaatcatgag aaaactcaga tatgttccga ttctgattat
    18421 gatagtactg ggagcttcgc tgggaggtat agcctttaat gcatttttga ttcctcataa
    18481 gcttttaagc ggaggcgtca gcggggtagc gctgatatta aactatattt tcgggctgaa
    18541 tcccggagtg cttatattta tattcaacat tccgatattt cttgcaggct ataaatttat
    18601 ggagaaggag tttattctgt taagccttgt cgggatgacg gttttttcag tctccattga
    18661 tttgttctca tttttgaaga atgttgttta tattgaggat acgctgttgt catgtttata
    18721 cggaggtgta cttaacggaa taggaatggg gctggtattc agaaacaggg cttcacaagg
    18781 cggagttgat attgttgcag ttatagtcaa aaaatatttt tccatgaatc ttggaaaaac
    18841 ttcattttta ataaatataa tagtaatagg aattgcttca ctctttttcg gactggagcc
    18901 cgccatgtac acattggttt ctatgtatgt ggcatccaca gttcttgata aagtgcagca
    18961 gggcttcgga agcagcaaat cagtaatgat tattaccgac aatgaacagc aggttgcaga
    19021 tgcaatacta aaaactcttg acagaggtgt tacctatctg gagggggaag gagcctacac
    19081 aggtaataag aagagggtta tatactgcat agtggcgctg aagcagttgg ctaagctgaa
    19141 gcagatagtc aacgagatag acagcagtgc ttttatggcg gtgagcgata cagcagaagt
    19201 gctgggctac ggcttcggga acagggggat ataaaagccg caaaaaacgg ataacactga
    19261 attcactgaa atccactgaa ctcttcattc cgtggatttt catgttttta gtgttacccg
    19321 ttattcttga aatttatcta caaatcaaat tcctttgcga tagcctcaat tgccttcttc
    19381 tggtcatcag ggctgataac gtaggatatt ttgatttcag aagttgtcac aatgctgacg
    19441 ggtatgtcgt tctctgcaag gaggttgaat actttggcgg caacacctga ctgagtgttc
    19501 attcccaagc ctacgacaga aagttttgtt atattgtcac aggtttcata agtgaaggtg
    19561 aataattttt tcttgaatgt gttcagtacc tttgaagcct gataaagatc agtcttgggc
    19621 agagtgaagg atatgcttac aagcttgttg accgggaagg tctggcttat catatcaata
    19681 tttatgtctt tgttagcgat gctttggaat atttccgcaa ttaccttgat atcatggggt
    19741 acaccgttta aggtaattat tgcttcatca ttattaaccg ccaaaccggt tattgccgta
    19801 ttttccatgg tattgtccat ctcctttatg attgtacctg gtatatcacc cctgcttagg
    19861 gctacaagaa caggaacctt atatttttct gcaagttcta tagatcttga atgcatgaca
    19921 cttgccccgg agcttgccat ttcaagcatt tcttcatagc tgatgcagtc aagcttcttt
    19981 gctctcggat aaagccttgg atcaatagta tatataccgt caacatcagt atatatttca
    20041 cactgggccc cgagctttgc ggcaagtgcc actgctgtcg tgtctgagcc gcctctgcca
    20101 agtgtagtaa tctcattata ttcatttact ccctgaaagc ctgcaactat gacaattttt
    20161 cctttcctat gctcttcacg gattttttct tcattaatat cagctatttt ggatttcgta
    20221 tgagagccgt ttgttcttat cccaacctgc tggcctgtaa aagaaacagc gtctgcatcc
    20281 aaattgttga gcgcaataga taatagagac attgaaacct gttctccggt tgccattaaa
    20341 acgtccaatt cccttttatc aggatcgggg ctgatttcat gtgccatatt tatcagatgg
    20401 tctgtagttt tgcccatggc agatactaca attaccatat cattgccttc cgattttctt
    20461 tttacaatgt gttctgctat attatttatt ttttcaattg tttcaacgga ggtgcctcca
    20521 tacttttgta caattattgc catttgtgtt cacatcctaa cctcttagat catccaataa
    20581 ttttttctta tcctcgttca aatccttaac gctttttatc attcttgcag gtattcctgc
    20641 cacaaccgta tcaggctcta cattttttgt taccactgaa cctgccgcaa caactgctcc
    20701 ctttcctata cgcacgcctt caagcacaac ggcatttgca cctataagaa cgttatcttc
    20761 aattatcaca ggatcggcgc ttggaggttc aaggactccc gcaattactg aacccgcccc
    20821 tatatggcag ttgctgccta taacagctct ggcaccaagc actgcgttca tatcaatcat
    20881 agtgttttcg cctacttcgg caccgatatt gataactgca cccatcatga ttacagcgtt
    20941 cttgtgtata agaaccttat cccttataac tgcacccggc tcaatcctgg catcaacata
    21001 cctggtatca agcaggggaa tagctgaatt tcttctgtca tattccaatt cgaagctcct
    21061 tattaagctt ttattatcgg agagaaattt ctccatatcc gaatgatccc cgaataaaat
    21121 ccagaagttg tccgcaccgt atgcacgtat gccgctcata tccgcatcct tcaggtcacc
    21181 gttgacataa gccttgacag gggtaacttt tttggattcc tttatatatc tggctatttc
    21241 atatggattg gtaatatcca atctttgatt catcaaatca gctcctcaag cattgttttc
    21301 atagtgtaat atcccttttg ttttgaagct atataacgtg cagccctgat tgcaccgcgt
    21361 ccgaacatat cacgggacag ggcggaatgc tttatttcga ttacttcatc cttaccggcg
    21421 aaaaatactg tatgttcacc gggaatggtg ccgcctctta ttgcggatat cccaatctca
    21481 ttgggctttc tcttttcacg gaccttcgac ctgtcataaa caaactcgag gccgtcatta
    21541 atccggcttt tcaattcatt ggctatcatc attgcagtgc cgctgggagc atcttttttt
    21601 tcgttatggt gtttttcaag tatttcaata tcgaattcat cccctaaggt tgcggctgct
    21661 tttaaagcca aatcaatcaa taccctgatg ccgaagctca tgtttgctga tacaaataca
    21721 ggtatggatt ccgatgcctt ctcaagcatt ttcagatctg tctctgaaag ccctgttgtt
    21781 cctactacaa gagctgtatg atgcttgaca cagtaatcta cagtctcggg tatcatttca
    21841 ggatttgaaa agtccactac aacatcagct ttgacttcgg cttccttgaa gctgcggtat
    21901 acaggataac tgtttggcag ccttgatgga tttcttgtca cacctgctgc tatactaagg
    21961 tcatcacatc cctgggcaag ccttgtaatt acctggccca tcttgccgtt gcagccgttt
    22021 atcattagct taatcaagta cgctaccccc ttaattcaaa tccggagttt atcagttcgt
    22081 tcttaagcct gtttaaattg ccttctgaca tatccacaag gggaagcctc agtttgccga
    22141 cattcatacc gagaagattc atagcggtct ttatcggaat cggattgacc tcacagaaaa
    22201 gggcatcatt cagcttcttt atgtcaagct gaagcttcat ggcggttttc acatcaccct
    22261 tcaggaaact gtcaaccata tcatgcatta gcttaggtgc tatattggcc actaccgaaa
    22321 taacaccgat tccgccgacc gagagaagag gaaccacctg gtcatcgttt ccggaataca
    22381 tgcagaaatt atcatcgcag aacattccta tttcaacaac ctggcttata tttccgcttg
    22441 cttcctttat gccgccgatg ttctcgtgga cggacagagc ctttaaagta tctgcgtttg
    22501 tattaattcc tgtcctgccg gggacattgt aaataattac gggaatgttt atggaatccg
    22561 ctattgccgt gtagtgtgca ataaggcctt tttgcgtggt tttgttgtaa taaggagtaa
    22621 cgcacagaac tccgttgcag cccacagact cagcatattt tgacagttct attgagtgat
    22681 gggtatcgtt gcttccagtg cctgcaataa ccggaatcct tccggcaatt ttgtccactg
    22741 tgaatttata tgcagctttt ttctcatcat ccgacattgt gggtgcttca ccggtggtgc
    22801 cgcatataat tattgcatct gtaccgtttt caacatgcca atccaataat tctccaagtt
    22861 tgttgaaatc tactccattg tcattgaaag gggtaacgat tgcaacccct gaacctttga
    22921 atatagccat tccaaatcct cctcacattt atatattatt tgccgctgat taacagctca
    22981 gctatctgta ctgcgtttgt ggccgcaccc tttcggatat tatctgcaac cacccagagg
    23041 tttactccat aatctacgga ataatccctt cttattcttc cgacaaaaac ttcatctctt
    23101 cctgctgcat taattgcaag ggggtagatg cagtttgccg gatcatcctg cacaattatt
    23161 cccgggctgc ctgcaagcag tcctttaagc tcgtcaatat caaagtcatt ttcaaattcc
    23221 gcatttattg attccgagtg tgcgtaataa acgggtaccc ggacagttgt cgctgtaatt
    23281 ttcaagcttt ggtcatcaaa tatctttttg gtttcatcga ccattttcat ttcttcctta
    23341 gtatatccat tatccaggaa cacgtctata tgagggagac agttataggc gatgggatgg
    23401 ggatatattt ttggagcatt cccttttatg ccctcctcca gatcgacgac tccttttaca
    23461 cctgtccctg agactgactg gtatgtggaa tatacaattc tttttatttt atatttgtca
    23521 tgaagcggct ttaaggcaac aacagcttgt attgttgaac aattagggtt tgaaattata
    23581 ccgctgtgat tttttaccgc atgagggttc acttccggaa ctataagggg aacatcgggg
    23641 tccattctga aagcactgct gttatccaca acgacaacac cttttgaagc tgcaacgggg
    23701 gaatattttt tgctggtttc ccctcctgcc gagaacagcg caatgtctat gtgcctgtcg
    23761 aaggattttt cgctaagctc ttcaaccaca tattcctttc cgctgaaagt gattttggtg
    23821 cctgcagaat ttacactggc aaaagcataa aagtttttaa ttgggaagtt tcgctcttcc
    23881 agcaccttga gaaacattct tccgaccacg ccggttgcac caacaactgc tacatcatac
    23941 ttgttcatct aattgcctcc tttaaaatta aagtgccagc ccggtaaaaa agctgacacg
    24001 aaaaagatat ttattcagaa caaaactgaa caaacacact tattcatgta tagctctcca
    24061 ccggaggttg tgcatagact ctccggtgac agtccgaact ctgtttgagc cgggaccagg
    24121 tcagaaatcc gtggcatatt tccgccttcg gcgcttttac ctttctggaa attcgggaag
    24181 ccacatccca attttgaata cccatactat ttaaaagcgc gcctctatcc tagaatagaa
    24241 acttttctta aattagaata atactatcat agggtttggg acgtgtcaac agctaatatt
    24301 aatttttgct ttatttattg ctatgataac ggttacaata gtattcatac tgcttaacat
    24361 cgaaagacaa aaatttgaaa tacagaacca tataatatac aatatattat atgaataact
    24421 ggaaagtact cagcaaggag tgatattttg ctgcatattg atgataaagt acaagcactt
    24481 aaggatgagg ttatctctat aagacggcat ctgcacagga ttccggaaaa agggctcaat
    24541 gagcatgaaa ccaccgggta catatatgaa tatttgaagg atttgggaat aaacgcagaa
    24601 ataagtcctg caggaaccgg tgtaatagca tacattgaag gcaagcccgg gtcaaaggcc
    24661 atagctttca gagcggatat agatgcgtta tcaattgaag aacagaccgg agttgaatat
    24721 tgttcaaaaa acgaaggctt tatgcatgcc tgcggccatg acggccatac tgcagtgctg
    24781 cttggttttg ccagactatt ggttaacatg ggtaacgcat tggacaataa tgtgctgctg
    24841 gtattccagc ctgcggaaga aggccccgga ggagcggaaa taattgtaaa aaacggggcg
    24901 ctggaacaat atgacgcaag ctgcatattc gggttacaca tatttccgga tatagaagaa
    24961 ggcaagatag gttgccggcc cggagcacta atggcacaga caggggaatt cgatctgtac
    25021 atagaaggca agagctgtca cggagcaatg ccccataaag ggaatgatgc ccttgttgcg
    25081 gcttcaaatg ttatcttggc ccttaatacc atagtcggca gatttacgga tcctttggag
    25141 cctgcagttg taacagtggg gaaaatggtc agcggcgaaa ggatgaatgt aatcgcaggt
    25201 gaagcattac tgcagggaac catgcgagcc ttcaacgata cgacctataa tactttaaaa
    25261 gaccgtgtca taaaaatggc tgattctata agcggagcat acgggtgcag ctgcagatat
    25321 gaaatacgtg atatgtatcc cgctgttcac aatgatgaaa ggctttacaa aatccttgcg
    25381 gaggatatag gagatgaaga ttttgtatat atgaatccga taatgcttgc agaggatttt
    25441 tcatattatc agaaaaggat acccggtttg ttttttatgc tgggggcagg caacagggag
    25501 aagggctata tacatcctct tcacagcaac atgttcaatt tcaatgatga aatattaatt
    25561 acgggagtac aggtttttta taatcttttg agaagatgca agcaattcca ataatttatg
    25621 ggatgcagca catatgaaga aaaggaggtt gggtaatggg taaggtaata agattacttg
    25681 tttttactgt tctggtaatg ataatgtttg cagtggctgc atatgcagac agtattgaca
    25741 cgggctggaa gggctatggg ctttataacc ttaacaagtt ggatgtcact attaccaatg
    25801 agtcagacaa tattacaata gacggggaaa aagtaaccgc cgtatacgag tacaccataa
    25861 agtccaatgc cgataaggat atagcagtta atttcggtat accggataat ggaattgtca
    25921 agttcagcat atatgacgga agcaaattcc tgaactacaa gaccagaaat acttcatacc
    25981 tcaaaagtac ctacggagca gagaatcttc agacccctga cggcagatgg tatttattca
    26041 ctatggtatt tacacccggg caaacccggt ctataagcat aaacattgaa gctcaaatga
    26101 caaaagagga aaatgataca tacgggttga gcttcttcaa ggatagaggc tattcctacg
    26161 cgataaaaag tgagaaaaca tggataactt tgaatttgtc agattttaag ccatatgaca
    26221 tatatgaact tgaaggagta aaggaagggc aaatatccga taatggggtt gctacacttt
    26281 ccttcagcgg agactatggg aacggagtat tcctgagata ccagcccatt gacaaaatgg
    26341 ctttggacag ccttgggaaa agtacgcaaa aaaagccgaa atccatagtc aaagccttta
    26401 atgaaaaaaa atacgtggaa gcgcttaatc tttgcaatga atacataagt tcaccctctg
    26461 acagcagcct gaatattgaa caggtaaagt ttataaaagc agaatgtatg aggttgtcag
    26521 gtaattatgc agagtatttg agcacagtgg agcagcttga catatctcag ctttaccccg
    26581 gaaggatacg gttcaaaata ctgtatgaca ggctgacagc atataaaaat acagaaaatg
    26641 ccgaaggggt gaatactata cttaaagagc tgattcctga aactcagcaa atctatcctt
    26701 ttctttatca ttggcttgac agaaacgggt acaagctttt agaagccgag ccggatgcag
    26761 aagatgaagc atccctgaat aatcaggtaa gcactgaaaa taagggtttt gatatactcg
    26821 gggcggtcat tggttttttc gcttttgtta ctgaaagcag atggacatat gtattattag
    26881 gctttattgc cggcttcatt gccggcttca ttatcggaag gattacaaaa agaaggaaga
    26941 aaagcagatc tgtttatctt tttagggatt aaaacagaat aatgcaagaa aaaattcaca
    27001 taatattgct atccaatgca ctaaaaaaca attcttgagg aagtgatatt atgaagtcaa
    27061 aaaagacaaa gaaaaaacaa atcatactga cccctgagga taaaatgaaa tatgaaatag
    27121 caaatgaact tggccttgga gagaagctgt ctcaagtcgg atggggaggc ctgacggcag
    27181 aagagaccgg taggataggc ggacttatta ccaggaagaa gaggcacttg ccaagctgat
    27241 cattagcata attacagccg catgatgcgg ctgttgctct taagcgcaat cgatgaaagg
    27301 atgggtatcc gatggagatt tataaaaaag ccgcagaatt aataaaaaaa tgcaagcctt
    27361 cagtagtgtt gacaggagcc ggaatttcga cggaaagcgg tattcctgat ttcagaagcc
    27421 ccggcagcgg actgtgggag aagatagacc ccatggaggc tctgtcaacc gaggtattgt
    27481 ataaaaaccc aaaaaaattc tatgatttag gttttagcat tataacttcc atgaaggata
    27541 gccttccgaa taaggctcat tatctggttg cggaactgga ggagaaaggg ctgattaacc
    27601 ttgtagtgac gcagaatatt gacggattgc atcataaggc aggcagcaga aatgtatacg
    27661 aggtgcatgg aaccgtgagg acctgcagct gtgacagctg cggagaacta tatgataccg
    27721 gcataataga aaaacggata aaagcgggag agattccgcc aaaatgcaga tgcagaggga
    27781 taatacggcc tgatgttgtg cttttcgggg acatgctccc cgactgcttt tacgacagca
    27841 ttaagaaggt tgaagaggcg gaacttctga tagtgatcgg atccagcctc tcggtgtcac
    27901 ccgtaaacta tcttgcggaa atatgcaaaa ggctgttgat aataaacctg ggagagactg
    27961 gttatgacag gaagagcgat ttaataataa gggaaagcac atcttccgcc ttggaaagga
    28021 ttgtgagctt catatgaact gttataatgt gctggctgag ctttatgata tgtttatgga
    28081 ggaaattgac tataaagcct ggtgcagata tgtggaggac atttttaaaa gctgcgggat
    28141 cagaccggca agagtacttg atactgcctg cggcacggga aatattacaa tccaaatgtc
    28201 aaaatccggt tataatctat gggggcttga catatcggcc gatatgctga ctgttgcgga
    28261 gaacaaggca agagcagcga agcagaaaat aacatttttg aatcaggata tgacaaagat
    28321 gaatttaaag gaaaaattcg acgcagtgct ttgcatgtgt gacggcgtta attatattaa
    28381 tgatgaggaa gccttgaaaa attattttaa gctggtttat gaaaggcttg aagacatagg
    28441 gattttcatt tttgatataa gcagttatta taaaatcaaa tacataattg gtaataatac
    28501 cttttacgaa gaaaaaaata ataagcatta tatttggaat aataattttg atgaaaaatc
    28561 tgagactgtg gaaatggacc tgatattttt tgttcccgag gaagggctct acagaaagtt
    28621 cagtgaatat catgtacaga aagcttacag gagcgaatac ctggccaggc tgctggaaag
    28681 tacaggcttt gaagaagtaa gctgcttcga cggtttcagc tttaacaagc ctgaggagaa
    28741 aagcgaaaga atcttttttg ctgctttaaa gaaatagaat gttgaatgat aactggagga
    28801 aataatgaag gattatgttg taagaggagc aagtattgac gacagcgtaa ggtttttcag
    28861 ctgtattacc acgaatatgg cggaagaggg aagaaaaatc cataactgca gtccggtttc
    28921 aattgcagct ttgggaagaa tgctgactgc gggaagtatg atgggaatta tgcttaaatc
    28981 ggatgaagaa accataactc tgcagataaa tggcaaaggg ccggccggaa gcatagtagt
    29041 catatccgat agcacaggaa ttacaagggg ctatatcaca aatccggatg ttgaattaat
    29101 gcatcgggag aacggcaagc ttgacgtggg aagggcagtg ggtgttgacg gcgtctttac
    29161 tgttattaaa gatatgggaa tgaaagagcc ctatgtgggg caaataccga ttgtaacagg
    29221 agaaataggc gatgatatat cctcttactt tgccgtatcc gaacagatac caactgccgt
    29281 tggcctggga gttctggtag ggatagactg cagcgtagcc gctgcaggag gattcataat
    29341 ccagcttatg ccggaagcag accatgatat gataacaagg cttgaagacc ggctcaggga
    29401 aatacgctcg gtaacggaaa tgatcagttc ggggttggat gccgaaggta tgataaagag
    29461 cattttaggc gatattgaat ataagatatt ggaaaagaaa caagtaggct acaaatgcag
    29521 ctgcagcagg gaaaggatgg aaagggcgct gataagcatt ggcaggaatg atttggagga
    29581 gcttatacaa gagcaaggat gcgcggaaat cgtatgtcat ttctgcaata aaaagtattt
    29641 ttttgaaaag aatgagctga ttgagatgtt tgaaaaagca tcatcaagta tataatatag
    29701 tatgctaaag attatcaaag gaattcagtg caataaaatg ataaatgtaa gaaatacaaa
    29761 aaattaagtt ttgactttag attatctgta ggttatacta aatgatgtcg caagtttcag
    29821 cgggggcata gctcagctgg gagagcatct gccttacaag cagagggtca taggttcgag
    29881 ccctattgtc cccaccaact ttttccgctt cgcggaaaaa gaccgacatt ccttgattta
    29941 atgcgtagca cttgcggagc aatttatgac ccgcatagcg caaaattaaa aagaccgaca
    30001 ttccttgatt taatgcgtag catttgcgga gcagctttaa ccaaactact tcgcggaaaa
    30061 agaccgacat tccttgattt aatgcgtagc acttgcggag gtaataagtg gcccagtagt
    30121 tcagttggtt agaatgccag cctgtcacgc tggaggtcga cggttcgagc ccgttctggg
    30181 tcgccattta atattatgcc ttggtagctc agtcggtaga gcagaggact gaaaatcctc
    30241 gtgtcggtgg ttcgattccg ccccaaggca ccatacaatg cgggtttaac tcagtggtag
    30301 agtgtcacct tgccaaggtg aaagtcgcga gttcgaatct cgtaacccgc tccaaatttt
    30361 atggcgacat agccaagtgg taaggcagag gactgcaaat cctttacccc cggttcaagt
    30421 ccgggtgtcg cctccatttt taacttcaac ttattgaata caataagttt ttttgttttt
    30481 atatgtgtct aagggcgtgg agaaatgcaa ataccgacgt ggggaggaca cacttcccca
    30541 acagtcggag tatttcacat tctccatata ttttattcag aaaagagctg gtacatatga
    30601 aatttttaaa aacttccttt atacccgatt ctgacgtaag ccttgcaatg gtggattaca
    30661 gaataagccc taaaattgaa gaaagcctta ataaaatagg aatagaatgc ttaaagacag
    30721 tgagatgccc tgaactgtat gatgcggtgg acgggcaccc ggatatgctg atgttccata
    30781 taggaggaaa caggatagta ctggctccca atgtttatga aaagatggcg ccaataattg
    30841 agaaaaaagg atttgcagtg acaaagggtg caacttggct ggttagaaac tatccgggca
    30901 atatcgcata taatgtattg agaatcggga aaacggcatt tcataatata cggcataccg
    30961 atgcggaaat aataaaggct ctggaaaagg aaaatgttaa aatgctgaat gtaaatcagg
    31021 ggtatacgaa atgctcggta tgtattttaa atgacaatac aattattact tctgatgaaa
    31081 aaatatcgga gatcgcagaa aaaaacggaa tagaaagctt tttaacaaag cccggaggaa
    31141 ttgatcttaa aggattgaat tatggcttcc ttggaggtgc cagcggctta gtatccaaaa
    31201 ataaaatagc atttacagga agcctttata ctcttgaaga taatagaaaa cttattgatt
    31261 atataaataa aaaaggtttt gaaattgtgc atttgtcaaa gggaagactt atggattatg
    31321 gttcaattct gcctttgaaa tgccgttaat tgaaacattt acagaccaag tataatgata
    31381 gtacacaaaa ctcatgaaaa taaagtgcgg cagcgttttg aaaaaattaa cctgtcactt
    31441 ggctttgaat acaagaaata tttaggcaaa tactaatcat tgaaagacga tttaatgatt
    31501 cctgaaattt ttttggatac tctccgtaca attaattatt tgcagtatcc ggcaaggagg
    31561 gagcagaaca aatgtttctt gtagcaattg gttccttgga tgattatgaa attataaaat
    31621 ctgaaatata cagacagttt gaaaatgcga aactggaagg cattgataca gtttatgagg
    31681 attatcagct tggaaataca cttttcataa gctgcggcat aaaagacaag cttgaggaat
    31741 tcaaatacat tctttcagat attcttgcag gagtgattat aggccattat gaatcaaagc
    31801 ttatcaggaa aattgtaaaa gaaaacttct tttatctgaa tggaaaagaa caagcaattg
    31861 ttatcgataa tgcatgcaag ctccttggtg aggaaaagca ggctcagccg gtaggattct
    31921 ataaagccga cagaaaaagt attatcagga gagaaatact taaatacctg gataccgaaa
    31981 aagaattgaa tatagaagga tttataaatt tccgcctcag tatgtatgtg aatgaactta
    32041 atgaaacagt tgaaagagca ttggagattt ttgtcgccga aagggaatat aacgagttca
    32101 taaaactgct gagatatttt gttgaaatac aggaatgcaa ggtagatact gttcatttgc
    32161 ttcaatccgg ggatggcagt tatctgttgt acgatgataa gaaaaacagc ataagcagcg
    32221 aatactttga tgagctgcgt tcggaaatac tggacgacac aatcaattat gacgatctgt
    32281 tgataagcac attaatcacg gtttctccga atagaatatt gatacatgat atagatgagt
    32341 ttaagaataa ggaactgatt caaaccatat ccaatgtgtt tgcggaaaga ataaccatat
    32401 gccgcagctg cgatttgtgc agcagtctgc aggaaagcag cgaaacacat agtgtaaaat
    32461 tactgtaggg aaaagaatga aaagagacca accttctgat agaatgaaga tgcaaagtca
    32521 aacacctaaa agaaggaggt ctctttggat atgattatac aacttttaac ggaagaaggc
    32581 attagaaatt tacaagaatc catattagaa ttggtgataa agggcgatgg ttttgcagat
    32641 gtagtagcaa aagtaaagga acagacagat gctttaggac gtgaaatatg caaacaaata
    32701 tttgagtctt tagatgagtc tatctaccag gatcccaaaa ggaaaaaaga ctggtatgtc
    32761 gagagaaaag ctgaatctaa gaaaattatt actcatttag gagaaataga gtataaacga
    32821 aactactatc gttcgcgcaa gggcaaagga tataggcact tacttgatga acttttaggt
    32881 atagatgtac atgaaagaat agatcaagga gtgagagcgg acatgacaga gcatgcagca
    32941 aatatctcgt accagaaggc aggagagtta gcatgtgatg taaaaatcag caggcaaaca
    33001 gttatgaatg gtgtacataa gcttaaggaa ttaaaggttg aatcattagg ggctgagcat
    33061 aagggaatag aaactttata tatagatgca gatgaggacc atatatcatt gcaaagtgga
    33121 aaaaaggtca tgcctaggct aatatatata cacgaaggcg ttaaaaaatc tggaagcaga
    33181 aatgaattaa taaatccctt ctactttgca agcctaagaa agaaccctga cgcattatgg
    33241 caggaagtat atgagcatgt agaggataca tacaatactg acattataaa gaccgtttac
    33301 atatcaggag acggagcagc ttggattaag aagggactgg aatattttcc gaaagccaaa
    33361 tttgtattgg atcgatatca tatgaacaaa tatgtaatta aggcaacggc taataatagg
    33421 gaatatagga gcaggatatg ggaagcattg aatttaagtg accttaaagc attaaagaaa
    33481 gtaatgaaag agctttatga tgaaacagag agcgaaagaa aacgtaaaga gatccgggag
    33541 agttttcttt actttaagaa caattgggat ggaatagaaa cctatggaaa agaaagggaa
    33601 agtatagtag gatgcagtgc ggaaggacat aacagccata tacttgccga tcgcatgagt
    33661 agcaggccaa agggctggag tatggaaggt gcggaaaaaa tggcaaggtt aagggcattt
    33721 aaagcaaacg gcggaaatgt atatgatttt atgaaggaac agaagaaaga aaagaaactc
    33781 tatgatatta ctacaaggct cttaaaagag acaaaaaaga agctcaaaca gaaaaagaca
    33841 gccgagataa ttggaaacat tgaagtcttc aaaataggaa ggataacggg actttataag
    33901 gcattgaaag agctctgctg agatatgttg ttgatatatt aatgaaggtg aagtctaatt
    33961 cttattttcc tatagtaaat tgacacgatc gcgaaacaaa caaatatagc ctgtgattgt
    34021 gaactgtggt ataataagcg taaagcatat tatactgctt tttttattgt atgggaaatt
    34081 atgctttctc gagagacgag ttttttattg taggggaatg tatgttcctt tgcatccggg
    34141 aggagaacaa atgtacgagg cactttttct gatagttgtc ggggcaatta ttggctggct
    34201 tacaaacgta cttgccataa aaatgctttt caggcctata aggcctttta gaattccgct
    34261 gctgaatata acaatacagg gccttattcc aaaaagaaga aatgacattg ccaaaagcat
    34321 aggcaatgtg gttgagaagg agcttgtttc aataaatgag atttttgaca ggctggtaac
    34381 ggaagaaaac aaacaggaag ttatagctat tataaaggaa aagctgctta aggtaattga
    34441 ctataagatt ccggcaatca taccttactc tataaagagc aggattattg attatttcga
    34501 cgaacagata aacaaggatg caatgtccat tcttgacagc tccgttgacg gaataatcag
    34561 caatacggct tataaagtca agataggaga aatggtggaa gaaaaaatag acgagctgga
    34621 actggaaaac atggaaagac tgatattgaa ccttacttca aaagaactga aatatattga
    34681 gctgttaggt gggctgctgg gtggaataat aggatttgtc cagacagtta taataagatt
    34741 gttataacgg gatgctcaaa aaatgccttt gttgcatttt ttatttgaat aatgtataat
    34801 ctaatacaaa taatatactt gattgctatg ataaggacga gtatagcaga gggttccaaa
    34861 agggaggaaa tgccgcagac tggaagcatt tcctggatta tctgctggaa aaccacctta
    34921 gagcgccgtg ttgaacaagc tgaagcttaa gtattcgcgg acgttgatta cgttacaatc
    34981 cagagtacaa atcagggtgg aaccgtggga aaatatcccg ccccttcagg gacgggagtt
    35041 tttgtttttt tgctgaaaat caaaggaggt atacagtatg gtgaaaataa ctttaaagga
    35101 tggttctgtt aaggaatata agaaagggac tacagttgaa gaaattgtcg cttcaatagg
    35161 aagcgggctc ttaaaggccg ccctggctgc aaagctgaac ggtaaggtgg cagatttgcg
    35221 cacccccgta aatgaagatt gcagtcttga ggttctgaca tttgatgatg acgacggcaa
    35281 gtgggcattg agacatacag gatctcatat actggcccag gcggtaaaga ggctttatcc
    35341 cgatgccaag cttgcaatcg gtccggctat tgacactggt ttctattatg acattgattc
    35401 ggataaatct ttttcggaag aagatctggc ggctatagaa aaagaaatgg agaatatcgt
    35461 aaaagaggat ctaaggcttg aaagatttga actgccgaga agtgaagctc ttgattttat
    35521 gggcgtgatg ggtgaaagct ataaggttga gcttattaac gaccttcctg aggatgccgt
    35581 aatatccttc tacaaacagg aggaatttgt agacctgtgt gccggacccc acgcaccttc
    35641 cacaggaaag gtaaaggctc tcaagctttt gagtgtggca ggggcatatt ggagaggcaa
    35701 tgagaaaaat aagatgctgc agaggattta cggaactgcc tataccaaaa agagtgaatt
    35761 ggaagaatac ttgaagatgc tggaggaagc taaaagaaga gaccacagaa agctgggaaa
    35821 agagcttgac ctcttcagcc tccatgaaga aggccccggt tttccgtttt tccatcccaa
    35881 gggaatgata ataaggaatg agcttgagaa cttctggagg aatatccaca ggaaacatgg
    35941 ctataaggaa ataaagactc ccgtaatact gaatgaggat ttgtggcatc aatcagggca
    36001 ttgggatcat tataaggata atatgtattt tactacaata gatgaaaaaa cttatgcggt
    36061 taagcccatg aactgtcccg gagggatatt gatatacaag aatgcaatgt acagctacag
    36121 ggatttgcct ctcaggatgg gagaattggg ccttgtgcac aggcatgagc tttcaggcgc
    36181 tcttcacgga ttgatgcggg taagaaactt tacccaggat gacgcacatt tatatctgac
    36241 ccctgaacag attaaagaag aggtcctggg cgtaataagc ctgacagact acgtttacaa
    36301 gttattcggg tttaaataca gggttgagct ttcgacacgg cccgaaaact cgatgggcag
    36361 tgatgaggat tgggagaatg ctaccgaggc gcttcggaat gcattggaag catccaaact
    36421 gccgtataaa attaatgaag gggatggcgc tttttacggc cccaagatag atttccatct
    36481 ggaagactgc atcgggcgta cctggcagtg cggaactata caattggatt ttcagatgcc
    36541 tgaaaaattc aaccttactt atataggtgc agacagtgag aagcacaggc ccgttatgat
    36601 ccacaggacg atattcggaa gcatagaaag atttataggt atattgatag agaattatgc
    36661 cggtgcattt ccggtatggc ttgctcctgt gcaggtaata atacttccgg tgtctgaaaa
    36721 acagagggat tatgccgaga agcttaataa gcagctggag gatctggaaa tccgcgctga
    36781 agctgatttg agaagcgaaa aggtagggta taaaataaga gaagcacagc ttcaaaagat
    36841 tccgtacatg cttgtggtag gagacaagga aatggagagc ggatctgtat cggtaaggga
    36901 cagaaaagaa ggagacgcag gagttatgaa atttgaagat ttcctgtcta aaatacaaga
    36961 tgaaataaaa aataaaacca ataagtaagt aaatatacaa gagtcaaaaa taaaaataaa
    37021 ggcaggtata acgattgttt gattgttaat tacctgtctt tgatttttat cgaattttaa
    37081 gctacaatca tattaacagg catgtattgc cgaaataatc cgtggaggtg aagtcatgaa
    37141 aatcggtaat aaaacattga tagtattatc agcctttgca attattatcg ctgtcatgtc
    37201 cggaaccttg aaggatgcgg cacgcggttc atacgataca gaaaaaaggc tgatagtagg
    37261 ccgtgcaaat gactcagtta ctttggaccc ttcctgtact gtcgaaatgg attccttcaa
    37321 agtaactgta aacatattgg aaacactggt gaaatgtgaa aacgggggaa atgaggtagt
    37381 gcctctcctt gcagaaagct gggaatcaag tgaggacggc ttgaaatggg tattcaagct
    37441 gcgcagggga gtaaagtttc atgacggaac tgctttcgat gctgatgcag ttgtattaaa
    37501 ttttaaccgt tggatgtatg aggataaccc ctatcataaa ggatcattta attattggaa
    37561 ttatattttc ggcggtttcc cgggcttggt aaaaagtatc actgcattaa acaattattc
    37621 ggtggaaata aaactgaaca agccctttgc tcctttctta tatgcaatga caatgccggc
    37681 attcggtata gcaagccctg aagctataaa aaaacatcag ggagatttat ctgaacaccc
    37741 ggtaggtaca ggcccctttg tctataggag ctgggagcga aataaaagta ttattcttga
    37801 gcgcaacgac aagtattgga acgatgccgc aaaagtagag cgggttgaat tcaaagttat
    37861 acccgggaat aaggatagat tggaagaatt gaggcagggt accattcata tagctgatta
    37921 tctgagccct aatgatattg caggcataaa gtatgatcca aatttgtact tgtacttcag
    37981 gccaagcttt aatgtgggct acatggcaat gaataatgag aaatctccat ttaacaaccg
    38041 attggtgaga gcggcaataa atcatgccgt tgataaggac aagcttatca atgatgtttt
    38101 tgacaatctt gcaaagcctg cggcaacctt cataccgccg tctctctggg gttacaacgg
    38161 aagcctggaa atctacgagt ataatccgga aaaatccagg cagctgctgg cggaagccgg
    38221 ttatccaaat ggattcaaca cgacaatatg ggtaatgaat gcttcaaggg agtattttcc
    38281 agacccattg aaaacggcac agtttataag ggaaaatctg cggcaggtaa gtattaatgc
    38341 agaaattgtg gtattcaact ggaatgaata tttgaaaaaa atacgtaacg gagagcatga
    38401 aattgcttta ataggctgga ccggagatta tgccgatccg gacaatttcc tttacacaat
    38461 gttggcttcc gaaaatgcaa agcccggact tgcaggcaat tattcctttt ataaaagcga
    38521 ggatgtagat caactgcttg cacaagcaag gcagacaacc aatatgtcat tcaggagaag
    38581 tctttacaga aggattcagg aaactgtgaa ctatgacatg ccttcagtac cgatggtaca
    38641 cacaatgcct gttcttgctg ccaggctatc ggtgaaggga tatactcctc acatgaccgg
    38701 tattgagtcc ttggaacaag tctcacttga aaatgagcga tgaagggtag aggatgggaa
    38761 ttatgaagcg aaatgtattt agaatggtac ttattattat acttgcccta ttgcttgttc
    38821 ttgtaaacat gccttataag aagaaagctg agacaatagt ttttactctt ccggggctgc
    38881 cacggcctat tgcaaaagtc ccggttgtaa ttacatctgc aggacagagt accgatacat
    38941 acttaattaa tgatatttca aaccaattga tgataagaag ctttttcatg ccccaggctg
    39001 aagctgaggg tatagaagat atgaattcaa ttgtttttgc ggtgggttac agttctttgg
    39061 gattaaagct ccagggcata agctacgaag atgaaaaagc acgtattgag aagcttatca
    39121 atatggcaaa ggataataag cttacaatca taactgttgt tttaggcggt gaacagccta
    39181 ttgacgtcag aaatgaggag attatcagtc tcgttggtgc caaatcggat tatatcatag
    39241 ggctgaaggg gtcaagcaat gaggaattac ttgccgattt ggcggagaat ggcaatatac
    39301 ccctgactct tgtaagaaaa gtgaaggaca tatctgtacc tttcgcttcg gcttttcgat
    39361 aaaggataat atttgtgaga ggttttacta tgactgccag ccaaaggaaa ataaatgaaa
    39421 acatattact aaagaccatt gctgcgggag gtgttttgct tattattaca ggcatgctgt
    39481 ttcctgtctt ttttcaaaac acctggttta atactattat gaaaatcaga gaagcgataa
    39541 ccaccggaga cagcgggcat ctcatacttg catctgcatc attgaacagc ctttgttcaa
    39601 ttcaaagcac ccttttgtat atgggatcca tactgttaat ttttaataca aaacttagca
    39661 gccgcttaaa atccatagat gtatttcttg tatcattagt cagtatagta tttttacatt
    39721 ggatggattc gctgatattt gaaataccct gggaacctgt gtcaacctta ttggcattga
    39781 ttatttcatt gtttcttttc gagaaactgt tcgttgaaac ttacagcttt attcaggttt
    39841 tcatagtttc ggtacaagtc ttttttgctt tccaatggct taatataatg ccttactttt
    39901 catcataccg gtttgggaga agcgatgttc cttacagcgt gaaaatatca gggatatatc
    39961 ttaatgccga taccgtacta aattttgcag ggtttgcgtt ttttatacct tttatcatat
    40021 cggcattcat tactgcaaca ctatttatca gttattcaag gaacattcat atgatgaggg
    40081 agaatcatat aaaagaaaac gagataaggg acatgagagc aaaggctctc gaaaaccaga
    40141 tatatcaaga ggttaaatcc ctggttcacg atttgaagac ccctttggtt actattcgcg
    40201 gcttgaattc cttgatggtt tccgaaaatg caaacggaaa gctggcggaa tacagtgaac
    40261 gtatagaaaa ctcagtagct aaaatgagtg atatgatttc cagcttccta tatgaatatt
    40321 cacgccagaa attgaaggca tctgacctta tcagttatat aagggcccag cttccgctgg
    40381 aagatgaaaa gataagattt tccatcagta ttgaggagga tcttccggat atatatataa
    40441 ataagataag aatagcaagg gctgttataa atgtacttga aaatgcaata gttgttccct
    40501 gtaaacattc atataagatg ataaactttg atgtgaggcg tgcagaaaag ggtatcaaca
    40561 taattataaa agacaacgga atcggcatag atgagtctaa tctaactaaa atatgggaga
    40621 taggctattc aaccaataat acgtcaggct tgggcctacc atttgcaaag cggattatag
    40681 aggataatgc aggaacaata gaagttggga gtcaacggga ttttggaacc actgtaacta
    40741 ttttcctgcc gtcagttgat aactttgaag attatggaag gggcgaggca attgaaaaaa
    40801 aataatacac cggaaattgt aataatagat gacgaacccg atattcttta taccataaaa
    40861 gaaatatgcc tgtatggagg ctgggaagca gctactgcgt ccaacggaag acaggggtac
    40921 gaactgtgca ggatgcacaa gcccagtctg gccatagtag attatcatat gccggattgg
    40981 gatgggctga caactgtcaa aagaatccgg cagacggaca atgccgtatc aatactggta
    41041 ttgactgtcg atgagaggca ggaaactgct gaaagattta ttgctgcggg tgcaactgac
    41101 tttgctatca aacctattaa atcaccggat ttgatttcgc ggataaaaat aaatcttaaa
    41161 ataaatgaga ttcaaaaaaa atatattttg gataagcagc agatttacct tgataaggga
    41221 atcagccctg ctactttgag tattatactt gattatctga aggagcaggg acacgggctg
    41281 acgattgatg atatagcaaa cgggctgaat cttgcgtacc aaacggtaca tagatatgta
    41341 tcttatctcc ttgaggagaa aaggatagaa atagtaccga tatacggaca actgggcagg
    41401 cccaaaaata aatacaagct gctataataa atgctataaa aaatattcca gagaaatatg
    41461 ttataaaaaa aggatttatt tatattttac agaataatca atatacattg tattagaaaa
    41521 aataatgttc tgttatcttt tttgcttttg taaataggga ttagtacccg gaaatacaat
    41581 tcccggtcta ataaataaca aaaattaatg gaggggaaga aatgaaaaaa gcattagcat
    41641 taatgctggc agttgtgatg ctttttagct tcacggcatg cgcaaaacca gcagagcctg
    41701 cagcagcaga gcctgctgct tctgaagcta aacccgagga agctaaggac aattggaaaa
    41761 ttgcaatagt caccaacact gtttcacaga acgaagaaga gtatcgttct gccgagtttg
    41821 tacagaagaa atacggcaag gataaaattg tccatgagct ttggccggac aatttcatga
    41881 ctgaacaaga acagatgatc agtgtattga ctaagcttgc tgcagatcct gatatcaaag
    41941 ctgtagtcat taaccaggct gtacctggaa caaatccggc gattgataag ttcctggaag
    42001 caagaaataa ggacgaagta cttattgttt atgcttcgcc tcaggaaaat gctccggacg
    42061 tatccacccg tgcagacgtc atacttcagc ccgatgagct gtccatgggt tcggcaatac
    42121 caaaccaggc attaaagcag ggcgcaaaga catttgtaca ttattcattc ccgagacata
    42181 tgtctattcc gatacttgca gagagacgtg agctcatgaa agcggaagct gcaaagattg
    42241 gtcttcaatt tgtcgatgct acagctccgg acccaaccag tgaagctgga gttacaggtg
    42301 ctcagcagtt catcctggaa gacgtaccga agatggtaaa gaaatacgga aaagatacat
    42361 cgttcttctc cacaaactgc tctatgcagg tgcctctgat caaagcagct tttgatgcag
    42421 gtgcaatcta tacacagcca tgctgcccgt caccttacca cggatacccg tcagctttgg
    42481 gtctgaatgt tccggaagat atagtcggcg gtttgcagga tgttattgac cagaccacga
    42541 agatcgttga agatgccggc ctaagcggac gcttctcaac ttggcccgta ccggcagcca
    42601 tgatggctac tattgcaggc gctgaatatg ccgtcaagtg gttgaacggt gaaactgacg
    42661 gcaagtttga caaaaaagtt ctggcagaat gcatgtctga ttatgcgggc atggatattt
    42721 ctctgaaccc ctatgtagaa aatggcgtta cttatgacaa ttacttactg ttcctggtag
    42781 acttccttac ttacggaaag cttccaagct aattgttgta taaagattta agttgcatcc
    42841 tggcaactga agtataactt gtaatgaggg tgtttcaata ctgttcggct tgagacatcc
    42901 tcattaagtt tgcaaaccgc tactcataag agcatatgtg gcaggaggga ttaacttggg
    42961 agacaaactc cttactataa aaaacatatc aaaagagttt tacggcaacc aggtactaaa
    43021 agatatcaat attgaaataa aaaagggtga aatccatgcc ctcataggag aaaacggggc
    43081 aggcaaatcc actttgatga atatattgtt cggtatgccg gtgattcatg aaaccggcgg
    43141 ataccagggc agcattgaac tggatggtaa agaggtcatg ttcaattcgc cccatgaggc
    43201 tatgttccat ggaattggaa tggtacatca ggagtttatg cttataccgg gttttaccat
    43261 tacggaaaac atcaaaataa accgtgaact gactaatcca aacctggtca gtaagatatt
    43321 cggcaaacaa tatgaaagct tggatatgaa gcgaatgaac agcgatgcca gaaaggctct
    43381 tgacattatg ggcatgggta tagaggagta cactctggtg gcagggctgc cggtagggca
    43441 tatgcagttc atcgaaatcg ccagggaaat cgataaaacc ggaatcaaac tacttgtttt
    43501 cgatgagccc accgcagttt tgacggaatc cgaaacccaa aacctgttgg ccgccattga
    43561 gaagttagcg gcacaaggaa taggcatcat atttataagc catcgtttgg aagaaattat
    43621 gcaagtttct catcgcgtca ccattttgcg tgatggggaa catgtggtaa caaaagatac
    43681 aaaggataca aacgtgtacg aaatcgcaga attaatggtg ggacgaaagg tatccctgga
    43741 tcgttcagat tcggattccc gccggattca agatgaatcc gtgcttagca tcaagaatct
    43801 tgaggtaaaa atgcccgggg aagaggtaaa aggttttgac ctggaggtaa agaaaggtga
    43861 aataataggc atcgggggcc tggccgggca agggaagctt ggagtagcta acggaattat
    43921 ggggattttt cccgccagag gccaagtaat ttttgaaggc cgcccactgg aactgaataa
    43981 ccccagggga gcaattgttg cggggataag ctttttaagc gaagacagga aaggcgtcgg
    44041 actgctatta gatgagtcca ttgagcttaa tacagttttt acgagtatgc agatccacaa
    44101 ggaattcctt tataatcttg gttttattaa ggttatggat tctcaaaaaa tccgcaatca
    44161 tacaaataag atgataaaag atctggatat tcgatgcaca tcctctttgc agcatgcggg
    44221 cagtttatcc ggaggcaacc agcaaaaggt gtgcatggcc cgggctctta cccagaaacc
    44281 gaaactgctg ctggtcagcg agcccacccg cggtattgat ataggtgcaa agaagcttat
    44341 tttggacttg cttaaaagcc ttaatgagga acaagggatg actatagtaa tgacatcatc
    44401 tgaattagcg gagcttagaa gtatttgtga caggatagct atcgtctgtg atggtaaagt
    44461 aaagggcatt ctgtctcctg aggcttcgaa cgctgaattt gggttgatga tgtccggcgt
    44521 tcacgcagat tcccaaatgg ggggtgctga ataaatgtta aacaatgaag ataagcaaaa
    44581 taatagtgtt aaaaagaacc tggatatccc tatagtaaaa agcattggct tgccaaccct
    44641 tattattgct gcatttttct tattggtaat gattacggca ggcgtactga aacaatcttt
    44701 gccgatgctc atcagcgata tattgcgtcg ttttggtatg aatggtgtat tggttttggc
    44761 aatggttcct gcaattaaat ccggaacagg ccctaatttt gctctgccct tgggcatcat
    44821 ttgtggttta ttagcctgga ctataggaat tgaaatggga tttaccggta ttgtgctcct
    44881 ccttataaca tcaataatag caacggtttt tgcggctgca accgggtact tatacggaag
    44941 gcttttaaat gcagtaaaag gcgcagagat gacaatcgca acttataccg gcttttccat
    45001 cgttgctctc atgtctttgg tttggctcat ggcacccttt aagagcaaac aaatgggttg
    45061 gatgatgggc aagggtttgc gtgaaacaat aaaactggat ggggtgaatg caggagagat
    45121 tctaaacaat tttctttcct ttgagataat tggagttaag ataccaacag gtttgctcct
    45181 gacgtttttt gcattttgct tccttgtctg gttatttttc aggacgaaaa caggcgttgc
    45241 aataatcgct ggcggcagca atcctgaatt tgccgctgcc tcaggtattg atattgataa
    45301 aaaccgagta atagccaata ttctttccac ggtcttaggt gccctgggaa taattcttta
    45361 ttctcagagt tatggttttt tccagctata ttcagggcca ttgatgatgg cgttcccggc
    45421 tgttgccggt gttctcatcg gcggagcaac cgcctcaaaa gctaatatct ctcatgttat
    45481 aatcggtaca ttcctattac agggtctgct tactgcatca atgcctgtgg caaatcagtt
    45541 gttcgcagga accgacttat ccgagataat gcgtgtaata ctgcaaaacg gtattattct
    45601 ttacgcactt acacaggtca aaggaggcgg taaataatga aaaaagccaa aaatatgctg
    45661 ggcaagcaga tgccgtattt aacagataat atagttccta ttatttttat tgctttcaca
    45721 ttgttaggtt ttgcactaac cgacaatgtg cccattaatt actttttatc tgaattatcc
    45781 agccgtttct atcgtaatgc tttcattgta ctgtctctaa taattcctgt tatagcagga
    45841 ctggggctga actttggtat tgtaatcggc gcaatgagcg gccagttggc cataatcatt
    45901 gtacggtatc tcagcattga atatctgcat cttagcggag tttgggaatt attcttttgt
    45961 atagccttgg cattgcccat tgccgctttt atcggcttcc tgacaggcag gctgtacaat
    46021 aagacccgcg gccaggagat gatagccagc ttaattgtcg gtttctttgc caatggggta
    46081 taccagttca ttcttctctt tgcaactggt gtaattatac ctgtccgggc tactcatccc
    46141 atgatcaaac ccgatggcgt cggagtgcgt atgacagtgg atatgggcga gctgcactat
    46201 tccttgcaga atataatgaa aacgcccttc atgtggacgg tactcgtcgt ttcggcagta
    46261 ttgttaatat tggttgttgc cctttacctt acgggaagga aaaggcctga taaaggcaat
    46321 agtgttatat ttattatcaa agtccttttg ctggcagcaa caattgctta tagtgcatat
    46381 gcaattgcga ctaataacaa cataacaaaa gtgaaagatg tacctacggt aaccattatc
    46441 ttcattggcc tgctctgcct tttcacagaa ttgattatga aaacaaagct cggccaggat
    46501 tttagaagtg taggccaaag tcagcatatc gcccagtcca atggaataaa tgtagacaaa
    46561 acccgaatca ttgcagttat aatgtccact gttatggccg cctggggaca gatattttac
    46621 ttgcaggata tgggtactct gaatgtctat ggtgcacata cacaaatcgg tatgttctca
    46681 gtagcggcat tactggtagg cggggcttcg gtgcgcaagg ctactgtatc acaggctttg
    46741 attggaacta tacttttcaa ttccatgttt ataatgtcgc ctgaaatcgg caaggctgta
    46801 tttggcaatg cccttttagg tgaatatttc aggacattta tggtatacgg cattatcggt
    46861 atggctttgg gcatctatgt ttggaatgat aacagaaaag cccgcatcac cttggagccg
    46921 atagagctga ataccccgga caattaaaaa aataggggat tgagataact cagccccttt
    46981 tttatcgtat aggaaattat aatttcctat acgatggggg atgcaagggg gagaagcccc
    47041 ctgccgataa aagagggtgc tcaggatgct tgcatccgtc gaagggaacc atagggttcc
    47101 ctagcgaaaa aaatgcgtcg tgagatgcat tttttattga agagcaaatt cattccgcga
    47161 gtataaaaaa aacttattaa aaaaaactac aggcaggtga tattttatga agcttgaaaa
    47221 aattgttgaa tcaatgaagg atgatattat aaaagcagct caggaactgg tgagaataag
    47281 aagcatcgaa ggaaatcccg agccgggtgc gcctttcggc aaagaggtaa gaatatgcct
    47341 ggacagaagt ctggatatat gcagtaagct tggctttaaa acagaaaatt tcgatggctt
    47401 tgcgggacat gccgaatatg gagaaggtga tgaaatagta ggcatacttg ttcatgtgga
    47461 tattgtacct gaaggttcag gctggtcaca gaatccatac ggaggcgatg ttgtcaacga
    47521 caaaatctat ggcagaggaa ccattgacga caaggggccc gcagcagcgg caatatatgc
    47581 attgaaagcg gtggcggatt cgggtgtgaa gttcaacagg agagtcagga taataatcgg
    47641 ctgtgatgaa gaaagcggca gatggggctg catgaagcac tacttcaagt atgcggaggc
    47701 tccgatgtgc gggttttcac ctgatgcgga ttttccgata ataaatagcg aaatgggaat
    47761 actaatattc aatctggaaa aagaatttga accgaatgaa gacagccggt gcggcggtct
    47821 gagaataaaa aggatacagg gcgggaacag ggtcaatatg gttccggact actgcgaatg
    47881 tgagcttgag atgaagaagg attttaccga cagagtcata aagactgtag aatatatgaa
    47941 aaatgaacaa aacatgaagc tggaaatgga gattcgcgat gataaatgta tcataaattc
    48001 ctacggaata tcggctcacg gagctacacc aggaaaaggt gtgaacgcaa tttcacagct
    48061 tatctcatgc atctgcaagc ttcctctttg cgtgtcggaa cagactgaat acgttaactt
    48121 tcttaactca agtatcggaa tggagataga cggaaaatcc ttcggagttg caatgaatga
    48181 cgaggtctcg gggaatctgg ttttcaatct gggcacaatc gatatgagtg ataagaaagg
    48241 aagcgtcgga ataaacatca gatatcccgt cacaaagaca gggaaagaag tatatgatat
    48301 catcagaaga aagtcatccg aagccggaat caaggtcatt gaaggaaacg gccaggatcc
    48361 cctctatgtt cccgcagata attttatgat taagatactt caaaaggtat atgagaatgt
    48421 gacagggcaa aaggcggatt tgatatcaat cggcgggggc acgtatgcaa gagccataaa
    48481 gaacgctgtt gcattcggtc cgttatttcc gggaaaggaa gaaacagccc atcagaagga
    48541 tgaatatatt gaagttgatg acttgatgaa gtgcacgaaa atgtttgccg aggcgatata
    48601 tgagctggtt aaatagcacg ttgccaaaaa aaagtgttga catcgggttt gcgtagtgtt
    48661 attattttaa agcagcagag aaaaataact taatatattg atttaagaag aagttccact
    48721 tctcacctta cgactatgtc agtaaggtta tggttctcaa gaataaaact ttgggcaggt
    48781 ggatatttct acctgccatt ttaattttgc atggaggtga aaggttataa gcagcaacaa
    48841 agatctacaa attaacgagg aaatcagaga cagagaatta agaataattg attcaaacgg
    48901 agatcaactg ggaataatgt cttccagaca ggctttggaa ctggcagcag aaagacagct
    48961 tgatcttgtt aaaattgcgc cgcaggcaaa acctccggta tgcaggatta tggattacgg
    49021 caaattcaag tttgaacaga gcaagaaaga aaaggaagcc aggaggaatc agaaaatagt
    49081 aaacatcaag gaaattcgtt tctctcctac tattgaggaa catgacttga atgtaaaagc
    49141 aaaaaatgct caaaagttcc tgcaggaagg cgacaaggtc aaggtaacaa taaggttcag
    49201 aggcagagaa gtggatcatg taagctttgg tgaaaaagcc ctgggaagat tttatgatat
    49261 agttaaggat tactgtgtgg tcgaaaaacc tgccaaattg gaaggcagaa atatgataat
    49321 gattttagcg ccgaaacagt aagcctggta attctatgaa tatttcataa gttcaaaata
    49381 tgaccaatac agaaggagag gatttttaat gcctaaacag aagacccaca gagcatcagc
    49441 aaaaagattc agctttacca agaccggtaa agtaaaaaga gcatgtgcat ataagtcaca
    49501 tattcttacc aagaagacta caaagcgtac gagaaagctg aggaaaggcg cttatacgac
    49561 tgctacggaa gcaaaaatga ttagaaaatt agttccatac aaataagaga cagggaggca
    49621 gcataacatg gcaagagtaa aaagggcaat aaacgctcat aagaagcgta gaaagatact
    49681 taaactggct aaaggatacg tgggagccag aagtaaaaaa tacagaatag ccaatgaaac
    49741 tgtaatgagg gctttaagat ttgcgtatat cggaagaaag cttaagaaga gggattacag
    49801 aaagctttgg attgcaagaa ttaatgcagc agccagaaat aacggtatat cctacagcaa
    49861 gtttattaac ggactcaagc tgtcaggtat tgaaatcaac agaaagatgc tttccgagct
    49921 tgcaataaat gatgcagcgg catttacaaa gcttgttgat acagttaagg aaaagctgaa
    49981 cgcataataa gcatattaga aaaaggccga ataggtcttt tttcttttag cggttataaa
    50041 cgcatacaca attgagaaaa ttattagtat accggatttt agagcagcat cacagaggtg
    50101 attgaaataa tcggatttaa cataatgagg aaaaagatta caatgactcc gacacaaata
    50161 atagtcattg gatttgcttc aatgatattg attggtaccc tgcttctgaa tatgcctatg
    50221 gcatcgagaa acggacagcc ggttggatta ataaattcat tatttacagc tacctcatca
    50281 gtctgtgtta ccggccttgt tgtcgttgac acaggtactt actggacggc attcggacag
    50341 gttgtaatac tgttgcttat acagataggc ggccttggtt ttatgacaat gtccaccctg
    50401 ctttcgcttt tgctggggag acgaataacc ttaaaggaac ggcttctgat acaggaatca
    50461 ttgaatcagt ttgacctgga aggtttagta cgactgacga agaatataat aattgcgact
    50521 attatcattg aatttatagg tgcaatagtt tttacaattg cgtttatacc ggattacggg
    50581 ctgagaaaag gaattgcttt tggagtattt catgctgtaa ccagcttctg caatgcaggc
    50641 tttgatctta tcggaggctt cagaagcttt accccgtatg ttgatagttg tattataaat
    50701 ataaatacca tgtttctgat aataataggc ggtctggggt tttcggtatg gatggacatt
    50761 tacaaggcaa taaaagaaag ggacatgaga aatgtcacac ttcacagcaa ggtagtagta
    50821 tcgacaacta taatactgat tttgaccgga gctttattta tattcataat ggagcttaat
    50881 aatcccgata caataaaaaa attatcctta tcagggaagc tgctggcttc tttctttcaa
    50941 tcggtatcgc cgagaacggc cggttttaat acccttgata tggcggtgct tacgactccg
    51001 acaaagctta tgacgattat tctgatggtc attggaggat ctccgggttc aacagccgga
    51061 ggaattaaaa cagccacggc cgctattctt tttttaacgg ttatttctgt tattaaaggt
    51121 aaggaggata ctgaggtatt tggaagacat cttccccaat acctggttta cagggcaatg
    51181 tctgttagtt ttataagcct gagtctgatt gtaattgtga caatgctgct atcaataaca
    51241 gagaatgcgg attttatgac tgtttttttt gaagcagctt cagcctttgg cactgtgggc
    51301 cttactcttg gttattcacc gaatctgagc ccctttggaa aattaataat cagtatgacc
    51361 atgtttgccg gcagagtcgg gcctttaaca attgtattgg ctttggcaag aagagccatt
    51421 aataacagag gaaatgtcaa atacccggaa gaccgcatta tggtaggtta gaaaggaatg
    51481 aaggcaaaat gaaacaattc gtagtaatcg gtatagggcg atttggaggg gctgttgctg
    51541 aaacacttag cgatctgggg catgaggttc ttgtgataga taccgacgag gaagcaattc
    51601 agaaaatatc cgataaggtt acacatgcag ttactgctga tgcaacggat gaaagcgtat
    51661 taaagtcttt gggagcgaga aattttgatg ttgctgtagt ggctataggc tctgacatac
    51721 aatccagcat cttgattaca ctaatgctga aagaactggg agtaaggcat gtggttgcaa
    51781 aagcacagaa tgaacttcat gccagagtac ttatgaagat tggagcagat aaaatagtat
    51841 tccctgaaag ggaaatgggt gagagggtag cccataacct gatttcttcc agcatacttg
    51901 actttattga actttcggaa gaacacagca ttattgaata tgcagtaccg gaaagctgga
    51961 aagggaaagc tttgagagat ataaacatga gggcgagata cggagtgact gttgttgcca
    52021 taagaaatat gagcaatgaa agtattaata tatccccaaa agctgacagc gaaataaaag
    52081 acggagacat aatgattgtc ataggacaca atgatgacct gaaaaaactt gaaaggaaaa
    52141 cagagaaaga gatatgaaag aaataatctc aagcagctcc aataatactc taaagtacat
    52201 aaaatccctg caaatgaaaa aagcaaggga tgaatacggc aaattcctga ttgaaggaga
    52261 aaagcttttt tgggaggccc tggattataa tgtgccgata tcaatggttc ttatcagtca
    52321 aagctatgcg gagagcagca aacaccaaga gtatgccgct gctctcaccg gaagaaacat
    52381 accgctgcat tatgccgatg acagggtttt caagagagta tgtgaaaccg aaacgcctca
    52441 gggaataata gctgtagctg aaaagctgaa atatgatatt gacaatatta tctcgaagga
    52501 taagctttcc cttatattgc tgcatgaatt aagggaccct gggaatgtgg ggacaatcat
    52561 aagggctgcc gatgcctgcg ggcttgatgc agtggcaata tcaaaaggga gcgcggactt
    52621 atataatgga aagacaataa gggcaaccat gggctcttta ttccatatac ccgttattca
    52681 gagcgttgat actgcggaaa taatcgataa gctgaagcaa aggggagttg ttacaatagg
    52741 ggcggacccc cacagcagca ttaactgtac agagctgcca taccaaatga aatccgcaat
    52801 aattataggc aatgagtcgc agggaataag cagcgaaata aaagaaatgc ttgatataaa
    52861 tacaacgata ccgatgccgg ggagggctga atccctgaat gcggggatag cagcgtcaat
    52921 actgatgtat gagctttatg tgagaaaaag tttacagtca taattccaga gcaataacct
    52981 tgagacatgg ggacgtttcg cgtgtctcgc tttcagtgag acaggagaaa cgcccccgtg
    53041 tctcgtgact atacaagctt cttgcttgat tgtgcaaact tataactatg cagcattatt
    53101 tttcacttac taaataatgt aattatttaa aataaaaaga ggaaataata ggaataaata
    53161 gaattatata tcattaaatg ctaaaaaatg gagtgatatg atgatatata agacgaacac
    53221 ctgtgcttta tatgggattg aagggcatgt gattgaagtt gagactgact tgtccaacgg
    53281 gctgccgggg ttcgacatag tgggccttcc tgatgcggca gtaagggagt caagagaaag
    53341 agtaagagct gcaataaaga attccggttt tctgtttccg caaaaaagaa taacagtaaa
    53401 ccttgcccct gccgatataa gaaaagaggg ttcagttttt gaccttgcaa ttgcttcagg
    53461 aatactgaga gcatcaatgt acataacgaa tgaagcattg gaaggctttg cttttttagg
    53521 agaattgtca ttaaacggca gtgtaaaagg aataaaggga gtactgccca tgtgcctctc
    53581 cttaaaagaa aagggcttct ataagctgat tcttgcaaga gataatgccg atgaagctgc
    53641 ccttgtcaac ggtatggaaa tatatccgat aaatagcatt actgatatgg cagagttctt
    53701 aaacggaaat aggttcatta caccccataa ggttgatttg gaagcaattt taaggaacga
    53761 tactgccgaa acatatgatt tttcggaggt gcgaggccag aatgccgcaa aaagagcatt
    53821 ggaaattgct gcagcgggag cacacaatat tcttatgctc ggccctccgg gctccggaaa
    53881 gactatgctg gcaagaaggc tgccttccat actgccggat atgtcttttg aagaaagcct
    53941 tgaggtgaca aaaatataca gcatagcagg attgcttaaa gacaagctat atcttgtaac
    54001 aagaaggcct tttcgtgctc ctcatcatac catgtcggcc ggttcgctta taggaggggg
    54061 aagagtgcct catcccggtg aagtatctct ggctcactat ggcgtactat ttctggatga
    54121 aatgcctgag tttcaaaaaa atgtgctgga agtattaagg cagcctcttg aggatgaatg
    54181 tgtcacaatt tcaagagtag gaggtacaat aacatatccg gcgaaaatta tgcttatatg
    54241 ctctattaac ccatgcccct gcggctatta cggagatgaa tcaggaagat gcaattgcag
    54301 ccccaatcat ataaaaggct atctgggaag aatttcgggg ccgctgatgg acaggataga
    54361 cttgcatata gaggtgccct ccgtgaattt caatgacctg tcctgcagcc ctaaagagga
    54421 aagctccgca gccatgaaga aaaggattaa cagagccagg cagctccaac tggaaagata
    54481 cgaaaacgaa ggaatttttt ttaattcgca gttaaagccc aagcatatga cagcatactg
    54541 ttccataggg aaaaaagaaa aagagcttct caggaatgca tttgacaggt ttaacttgag
    54601 tgcaagagca tataatagga tattgaaggt agcaagaacc atagcggatt tggaagaaag
    54661 cgaaagcata ggaatcgatc acattgcaga ggcaattcag tacagaagcc tggacaggaa
    54721 gtacatatta tgaacaactc atgcgaggtg taagcgatga gcactaatga acgtgatata
    54781 tgctcggtaa tagaaaaact cccggtttac ggcaaattgc tgataaaact ttataaaagc
    54841 gctgatataa gaaaagggca aaagctttta ctttctgcgg gaatagctta cagcgtctcc
    54901 cctatagagc ttatacccgg tatcatacct gttgccggac agctggacaa tcttatcgta
    54961 atgctgcgct gtctgaggaa ggtacttaga tcttcggatg aaaaagccag ggaaagattt
    55021 ctcaaagagt caggtatgac aattgaagag attgatgagg atataaagat tagtgtggat
    55081 actttaaaat ccattggcag aggaaccgtc aggatagtca caaacacggg taagtttgcc
    55141 ggttactcgg taatgcgcca cataaaggaa tataaaaata aaagataatt aaaaaccggt
    55201 agcatcacac taccggttaa aaaataggag ggggaagaat taattccagt tatatccttg
    55261 atgattttat tatatcagaa caatattaca aagttgttat catagtgtaa caatttaatg
    55321 aagattgtaa caaaattgta tcaattcaaa gggggttgct atgaaagata aaaaatacct
    55381 tatgtggctg tcttctattc ctggagtcgg aagcaaaacc tgcctgaggc ttgtaaagca
    55441 tttcagctcg gctgaaaacg tatttcattg ctcattttca gagcttatga actctggaat
    55501 tgtaagggaa aagattgcag aatcaattaa agcggacaga aatacagaaa aagtaaatga
    55561 atattttaga attgtgaaag ataatgatat aaaagtctat actatatttg aaaatgaata
    55621 tcccgagaat ctgaagcaca tatatgatcc gccccccgta ttgtatgtaa agggtgaact
    55681 gacagccggg gattcaattg caataagtat tgtaggctca cggaaagcaa gcgattatgg
    55741 tctgaagacc gctcatagga tagctttgcg tctggcagag tcgggtatca caatagtaag
    55801 cggaatggcc atggggattg attccgcagc acacagggga gcaatagcgg caaacggaag
    55861 gacaattgca gtattcggat gcgggctgaa gtatgtttac cctgcggcta atttcagatt
    55921 gagcagggaa attgagaaaa ggggtgccct gatatcagaa tacccctttg atacggaggc
    55981 atatccttct cagtttcctg caaggaacag gataataagc ggtatgtctt tgggggtcat
    56041 tgtcgttgag gccggtgaaa agagcggatc ccttattact gccgattttg cccttgaaca
    56101 aggcagggag gtgtttgccg tcccggggaa cataacctct accaacagca aaggaacaaa
    56161 tgaactgata aagaacggcg caaagcttgt aagcagaatc gaagatattc tggaggaatt
    56221 taatattgaa cctatatgca gggaaaaaag caatacgttt gacaatagaa gctgcgaaat
    56281 aagcgctgag gaaaggctga tactggaatt acttcattca tgcggcggcg acagggatga
    56341 aattgcagcg gcatcagggc ttcaaaccgg taaggcgatg gctgcattga caatgctgga
    56401 ggttaaaggg ctgattcggc aaacaggcgg gaattaccat ttagtttgat tttttctaat
    56461 tttctgttat aatatgattt gaatttttaa aatgaggtga attatttgaa aacatcattg
    56521 ataatagtcg aatcacctgc aaaagtaaaa acgataaaaa agttcctgag ttcaagctac
    56581 aaggttgagg catcaatggg acatataagg gacttgccga aaagtcaatt gggtattgat
    56641 atagaaaaca actttgagcc aaagtacata actataagag gcaaggggga aattaccgac
    56701 aagctgcgca aagaggcaaa aaactgtgac aaggtatatc ttgctactga ccctgacaga
    56761 gaaggggaag ccatatcatg gcatctggca aatatactta atctgaacac cagggaagta
    56821 tcacggattg agtttaatga aataacaaag tcagcggtga caaaagcctt gaaaaatccg
    56881 cggaagctta atttcaattt aatagatgcc cagcaggcga gaagagtatt ggacagactt
    56941 gtgggataca aaataagccc gctgctctgg aagaaggtca aaaagggctt aagtgccgga
    57001 agagtccagt ctgtggcagt aaggcttatt tgtgacagag aagatgaaat cagaacattt
    57061 gttcccaggg aatactggac tttggcagca aagttgaatg atcccgatac aagaaagagc
    57121 tttgatgcaa aattcttcgg cacagaaaat gagaaaattg agatctcaag cgagagcgaa
    57181 atggaaaaaa tacttgaggc tcttaagggt gcaaaatata ctgtcaagag aatcaaaacg
    57241 ggagagaaaa ggagaaactc tccaaacccg ttcattacaa gcactcttca gcaggaagcc
    57301 tatagaaagc ttgggtttac tgccaagagg acaatgatgg tagcgcagca gctttatgag
    57361 ggtgtggata tcaagggcga aggaaccttg ggccttgtta cttacataag gactgattcg
    57421 gtaagaatat ccgaggaagc caaaaaagat gctgccggat atattatgaa taaatacgga
    57481 aagagtttcc ttggggggga gattaagcag aacaagtccg ggaagaaggt acaggatgcc
    57541 cacgaggcca tcaggcctac ttcggtttac agagaaccgg aaagcataaa agactccctt
    57601 gataaagaac aatacaaatt atacaagctt atttgggaaa ggtatgttgc aagccagatg
    57661 caggctgccg tattcgatac cgtcacggct gacattgaag caaacggcta tatttttaaa
    57721 gcatacggaa gtattttgaa gtttccgggc ttcatgagtg tttataaaga aagcagcgat
    57781 gaaaaggaag aagaagaaat atcactgccg agacttaccg aggggcagtt gctgaagctt
    57841 aagaaactgg agcacaagca gcattttaca gagcctcctt taagatatac ggaagctacc
    57901 cttgtaaaga ttcttgagga aaaaggaata ggcaggccaa gcacatatgc tcctataata
    57961 tcaaccatac tggcaagagg ctatgtaata agagacaaga aatttcttgt tcctaccgat
    58021 ttgggcgaaa aggttacggg gattatgaag gaatattttt ctgatattgt agatgccgag
    58081 tttactgcca atatggaagg gaagcttgac aatatagaag agggcgagaa ggattgggtg
    58141 catgtaataa gagagtttta cgggcccttt gaaaaaacac tgaaaagtgc ggaagaaaag
    58201 gtcgggaaaa taaagataca ggatgaagtc agtgatgtcg tctgtgaatt ctgcggcaga
    58261 aacatggtat ataagcaggg aagattcggc aaattcctcg catgtcccgg ttttccaaac
    58321 tgcagaaaca caaaggccat agttgagtca acaggcataa actgtccgaa ttgtacgggg
    58381 gaattaattg ctagaagaac caaaaaagga aggaagtttt atggctgcag caaatatccg
    58441 gaatgcagct ttgtaatctg ggacgaacct gttgccgaga aatgccctga gtgcggaagc
    58501 cttattgtga agaagtataa caaaaagggt tttgaataca aatgcatcaa tgaagcatgc
    58561 ggttacaaaa ggtcagataa taacgaggtg aaatagtctt gaaggaaaag gccgtagtta
    58621 taggtgcagg tttggcagga agcgaagcag catggcagct tgccaaaaga ggaataaagg
    58681 taaggcttta tgaaatgagg cctgtggcca gtactcctgc acaccatacc gataagttcg
    58741 gggaattggt ttgcagcaat tcccttcgct caaaccagtt ggagaatgcc gtgggactgc
    58801 tgaaagagga gatgcgaaga ttaggctctc tgataatgga atgtgccgac agcaaagcat
    58861 tgcctgcagg cggtgcgtta gctgtagaca gagagggttt ttcccaggaa ataactgaca
    58921 gaattaccgg taactccaat gtagagatta tacgggaaga agtaacatgc attcctgaaa
    58981 atgaatatgt gatagttgca acggggcctc taacctcgga aaagctttct ttcagcatag
    59041 gcaggatgat ggacagcgac tatttatatt tttatgacgc agcagctcct attataacct
    59101 atgaatcaat aaacaaggac aaggtattca tggcatcaag gtatgaaaaa ggtgacagtg
    59161 attatataaa ttgtcccatg tcagaggaag aatacgtgaa ttttcacagg gaacttgtaa
    59221 atgctgaaac tgcaccactt aagagctttg aaaaagaggt tgtttttgaa ggctgtatgc
    59281 ctgttgagac tatggcgaaa agaggattgc agacattgct gttcgggcct ttaaagcctg
    59341 tgggcttaac caatccccgc agcggaaaac agccttatgc agttgtacag ctaaggcagg
    59401 ataataagga gggtacttta tataatattg taggttttca gacacacctt aaatggggtg
    59461 agcagaaaag agtgttcggc atgataccgg gactcgaaaa tgcagagttt gtaaggtatg
    59521 gtgtaatgca cagaaatacc ttcataaatt caccgaaggt aatgaggccc acacttcaat
    59581 tccataacaa cgacaagttg ttttttgcgg ggcagatgac aggagtggaa ggatacgttg
    59641 agtccgcttc ttccggtttg gttgcaggaa taaatatggc caggatcata gatgggaggg
    59701 aacccgttgt atttcctgaa actacagccc acggagcatt gtgcagatac atcacggacc
    59761 gtacaataaa ggcattccag ccgatgaatg tgaattttgg aatattcccg cagcctgaat
    59821 acagaataaa aaacaaaaag gaaaaaagca gttattatgc tgaaagagct ttaaaggact
    59881 tggaaaaatt tattcgtgaa cggctctaag tggttgaaat atatatattc atatgctata
    59941 ttgaatctag cgagtttttt gacttttatg aatattgtaa catatttgaa atagattact
    60001 ggaggagaac tatgtttcat ggtacaacaa tagctgcagt caaaagaaac gataagggtg
    60061 cgattgccgg tgacggtcag gtgaccttca gccaaagcac cataatcaag tcaactgcca
    60121 aaaaagtgag aacattattt gacgggaaag tactggttgg gtttgccggc tcggtatcag
    60181 atgcttttac cctttccgaa aagctggaag aaaagctgga gcagcacagc ggcaatctga
    60241 aaagagccgc agttgagctg gcaaaggatt ggagaagaga taaagtacta aggcaattgg
    60301 aggctatgct gattgcaatg gataaagaaa ctctgcttgt tatttctgga agcggagagg
    60361 taatagagcc ggatgatggt gtaattgcaa ttggttccgg aggcaattat gctctggcgg
    60421 cagccaaagc tctgattcag aacacagatc tcccgtccca tgaggttgta aggaagtcct
    60481 tggaaatagc tgcctcaatt tgtgtattta ctaataataa catcattgta gaggaattat
    60541 aggaggaatc tatgaacgag ttaactccga aacaaatagt agcagagttg gataaatata
    60601 tcatcggaca ggataaggca aaaaaatcag tagctgtggc tttgagggac agatacagga
    60661 gaaatcttct ggatgaagaa atgcgggcgg aggttactcc caagaatata ttgatgatag
    60721 ggccaaccgg agttggaaaa acagaaattg cacgcaggat agccaagctc gttaatgccc
    60781 cttttgtaaa agtggaagct acaaaattca cagaggtggg ttatgtaggc agagatgttg
    60841 aatcaataat aagggatctc atagaatttt ctgtaaggtt ggtaaaggcg gaaaagatat
    60901 caaaggttaa tgaaaaggca tttgatatag cggcggaaaa ggttgccgag atacttgttc
    60961 cttcttcaaa caaaagcagc atgtccaaga atccctttga aatgatattt aattacggcg
    61021 gaagcaaaga aaccgaaagt gatgaggaaa cgaagaaaaa ggaagaaagt ttggctgcca
    61081 atcgatacta tgtaatgcaa aatgttaaga acggggattt gaacaacgag aatatagaaa
    61141 ttgagataga agatacatcc gggcacagca ttgaaatgtt atcggggccg gggatggatc
    61201 aaatgagcat aaatcttcag gatatgttcg gaggcatact tcccaaaaag actaagcgaa
    61261 gaagagtaac agtaaaggaa gcgttgaagc tgctgacgca gcaggaagca caaaagctca
    61321 ttgacatgga tgaagtaata gaagaagccg ttaaaaaagc cgaagagaga ggaattattt
    61381 tcatagatga aattgataaa attgccggga gaggcaatat cggcggccct gacgtgtcaa
    61441 gggaaggtgt acagagagat atacttccca tagttgaagg aagtaccgtt ataacaaaat
    61501 acggccctgt aaagacggat tacatgctgt ttattgcggc aggagctttt catgtggcca
    61561 aagtatccga tttgataccg gaattgcagg gaaggttccc tgtaagggtt gaattagaca
    61621 gcctgagcaa gaacaacttt gttgagatac taaccaaacc caacaatgct cttataaagc
    61681 aatacaaggc tcttctgcgt acggaaggaa taaatatcaa ctttgaggag gatgccgttg
    61741 aagagattgc atctattgca gcttttatga atgaacagta cgagaatata ggagcaagga
    61801 gactctatac ggtaatggaa agactttttg aggatatttc atatgaagcg cctgatatga
    61861 agagcggtga attgaatatt acgaaagaat atgtgcaaaa ggcattgatt cacgatatta
    61921 aaagaaaaga tgtcagcaat tatattattt aataaggagg ataaatatga gttcaacact
    61981 attagaaaag atgaggaaag tcaacaaggt actccagaca tcgggcactc aatccgtatc
    62041 ctttcatgat ctaagcaaaa tactaagtga ggtattaaac gcaaatgtat atatattgag
    62101 cagaaaagga agaatactgg gatactcatt gggcacctca gaggagtgcg aatttttaaa
    62161 gaacgaactg ctgacagaga agaaatttcc tgatgaatac aataaatcac tgctctcata
    62221 ccatgaaaca agatcaaacc tgataaataa agataaactg tgcgttattg acaacagaga
    62281 aaggtgcgat atgggtacaa attattctac tatcgttcct gttaacggca gcggtgaaag
    62341 gcttggtaca atgatgctcg caaagtataa ccaggaattt actgacgaag accttgtttt
    62401 ggcggaatac ggagcaacgg tagtaggcct ggagatatta agatccaaac atgatgaaat
    62461 tgaagaagaa gcaagaaaaa aagcagtagt acagatggca atcggaactc tttcttattc
    62521 ggaattggaa gccatagaac acatattcaa ggagctggat ggaaatgagg gcttgctcat
    62581 tgccagcaag attgccgata aagtaggtat aacaaggtcg gtaatagtaa atgcactcag
    62641 aaaatttgag agtgccggtg taatagaatc aagatccctg gggatgaagg ggacacatat
    62701 aaggattttg aatgataagc tgctggaaga actgaaaaaa gtacggtaga atcatcatta
    62761 aggattagtg gaatgctatt tggggacatg gggacgtttc tcctgtcccg ctttcagcgg
    62821 gacaggagaa acgtccccat gtccccttcc ccgtgtcccc gtcgcattct tctttttttg
    62881 cgaaattttt tagcggagga tgaaaggaat ttgctttttt ttcgataata tatatggaag
    62941 ttaaaattat gtaaataata tatttttatt ccttgaaaac ggaggaaatg ttgaatttat
    63001 gtcgaaatac taactgatat aggtatttct ggcaattaaa attctattat ggagttttat
    63061 ggaggttcct gttatgattg ataagataag caataagacc ttaatgcttg aaaaggctct
    63121 tgatgcggcc tggatgagaa atgaagcaat ttcaaacaac attgcgaatg ttaatactcc
    63181 cggattcaaa aaatcctatg taagattcga ggagtatatg gctgacgctc aggaaaagtt
    63241 ccagatatcc ggcgccaaga aagatccgag atttttgccg ataggcaagg atagagttat
    63301 atcggccaat cctgaaattg tacgggagaa ctccacttct atgagaagag acggaaataa
    63361 cgttgatata gatgtggaga tggcggagct tgctaagaat acaattaaat ataatgcttt
    63421 aatagctcag ttgtcaaagg aattcgctaa aataaaattt gcgattaacg ggaggtaatt
    63481 acaatggcat ttttgaattc aatcaatatt agtgcttccg gattaactgc agaaaagctg
    63541 agaatggatg taatatcaag aaatattgca aatgtgaata ctacaaggac cgcggaagga
    63601 actccttaca gaaggcaggt tgtggttttc cgggaagggg aaggcagtac gcccttcagt
    63661 gaatacctga gtgatgccag caggaggctt attggtgccg gtgtaaaggt taccggtatt
    63721 aaagaagata agactccctt caagtcggtt tatgatccgg gccatccgga tgctgatgaa
    63781 aacggctatg taaagatgcc aaacgtggat ataatgacag aaatggtaaa tatgatatct
    63841 gcaagcaggg catatgaagc caatgtaaca gcaattaact ccacaaagag tatggcacta
    63901 aaggcactgg aaattggaag aagataaaaa tgttagccat tttagggaag gaatgatatt
    63961 attatgaaaa ttaattttcc tgtcggacag tttggcattg attcaaattt gaataataat
    64021 gccgcatcca ggaaggaagc attatctttt accgattttt tgaataatgc aataaaggat
    64081 gcagacaagc tgcagacaga atcggaacaa ttgaatcagg cttttgcaat gggcttgaat
    64141 gataatctgc atcagattgt gatagcttcg gaaaaggcgg atattgcttt acaattcacc
    64201 gttcaaatac ggaataaggt tttggaagca taccaggaaa taatgaggat gcctgtataa
    64261 aaatttttgc ggtttcatga ggtgatggaa tgggtgaatt ttttaataat ttaaggaaca
    64321 ataccgtaga attctggaac ggattgaaaa aaggacaaaa agtgaggata ataattgccg
    64381 gattattgtc tttcgtttta attgtcagtt ttataacata tatgagccgg cctcagatgg
    64441 ggatacttta ttcggatctt agccagggtg atgcaggaac aattattgca agactgaaag
    64501 aaatgaatgt gaatacaaga attgaaggca ctacaattta cgttccggtt aagaggatag
    64561 atgagctgag ggctcaattg gctgttgagg gaatctttgg tgatgatata ttgtatcctg
    64621 aggaactgca ggccactttt tatgagacaa gccaggacaa gaaccaaaga tatcttatag
    64681 ccaagcaaaa caagcttaaa aagggtctga gcgctataga aggtgtagag tatgcggatg
    64741 tgaatctgta tataccggag gatcagactt ttataattga tcaaaacagc agtgaagcaa
    64801 ctgcatccct tataatcaaa atgaagccag gatatccggc tcttgacaga aatcaggtaa
    64861 acggtatcgt tcagtatatt gcaaggagcg ttaaaggttt aaaacctgaa aatatctcaa
    64921 taatcgatga aaacggaagg tcccttgtgc ctgatacagg aacacctaat tcaattattt
    64981 catcccagat ggaaatgcag gaagccgtgc aggacaaaat tgaaaaaagc attacaaaat
    65041 tcctggaagc tcccttcgga ataaacaatg tcaaggtaaa agcagcagta aagctgaatt
    65101 acgacagtgt tacacagaac ataacaacct atgaagctcc taatacggaa caaaacgaag
    65161 gcatcgtcag aaatatgcag gatataagaa aagaatgggt tgacgcaggt cagggaggcg
    65221 taccgggcac cgattcaaat accgacataa accaatacgc agaggtggat tcatccaaag
    65281 cacagtacaa tgaagcaagt acaattgtaa actatgaaat aaatgaaata aaagaacaga
    65341 ttataaaaga aatgggaagt atcgagtcct tatcagtatc ggtgcttata aatacattgg
    65401 attctgaagg gaatccgaag caaatcccgc aagaattgca ggatagggtc ggagcattag
    65461 taaaatacgc tttgcaaggt tttaattata cggcatccac agaagaaaat gagggtatag
    65521 aagtagtttt tgcgcccttt gatatggaat tagccaataa aattaaagct gatcaggaag
    65581 cagagaggaa agcagttttc tgggaacgta tcattatgat cggaacaggt gttgccgcat
    65641 tgcttgtctt tggaggagtg atatttataa tgttaagaaa acggaccgga gcctatggtg
    65701 ccggatcagg atatgctgtg gacagcaccg gaagggcaat gagtgaggct gcattatcag
    65761 gtgaacctat tgctgaaatt gatattgagg ataaaaatga agttaagaaa aagatagaaa
    65821 agtttgtggg gcttaagcct gagactgtcg cacaactgtt aaaaacctgg gttaatgagg
    65881 agtaggaggt tatgtaatgt caagaggatt aaaaggaact tttaacggca ggcagaaagc
    65941 ggcagttctt ctgataacac tcggacctga aaagtctgca gctatattca agcatttaaa
    66001 agaagaagat attgaacagc ttaccttgga aattgccaat atgagaacag taactcccga
    66061 ggagaaggaa gaggtactga atgagttcta tcagatatgt attgcccagg agtatatttc
    66121 cgagggtggt atttcttatg ccaaggatat acttgaaaga gcccttgggg gacaaaaggc
    66181 attggacgta ataaacaggc ttactgcctc acttcaagta agaccctttg actttgtcag
    66241 aaaagccgat ccggctcaga ttcttaactt catacaagga gaacacccgc agacaattgc
    66301 actaatattg gcatatctga agcctcagca ggcggctgcc ataatttcat ccttaccgca
    66361 ggacaagcag gctgatgttg cccgcagagt ggcaattatg gacaggacat cccctgaagt
    66421 aataaaagag attgaaagag ttcttgagag gaagctttca tctatggtta cacaggatta
    66481 cacaatcgcg ggaggtttgg ctgcggttgt agatatactg aattcagttg accgcggtac
    66541 tgagaaattc ataatggaga ccctggaagt acaggatatt gatcttgctg agcaaatcag
    66601 gaagagaatg ttcgtatttg acgatattgt aactcttgac aacagatcca tacagagggt
    66661 tacaagggag gttgaaaatt cagattgggc attggctctt aagaatgcag gagatgaagt
    66721 taaaaaagca attttcagta atatgtcaaa acgtcttgcc gagatgataa cagaagatat
    66781 ggaatacatg ggccctgtaa gactaagaga tgttgaagaa gcacagcaga agatagtgaa
    66841 tattatcagg aagctggagg atgccggcga gattgttata tctaggggcg gaggagatga
    66901 aataattgtc taaggtttac aaatcaagaa atataactat aggatttcct aaacatttag
    66961 ttcatgtctt caggaaagaa acaattaaag aggaagagtg caaaacaata aacaccgatg
    67021 ataacaatac tgaggatgaa gaagaatatg ccaattcaat agttgaagat gccaagcaaa
    67081 tgtatttgaa gataatagaa gaagcaaatt cggaagcccg cagaataatg gaagcagctg
    67141 aagaggaagc tcggagcctc ttatctaatt caagggaaga ggggtacaat gaaggttatg
    67201 aatccggata tcttgaagga aaaaacgaag cacagtcgat aattgatgaa gcttcagaaa
    67261 taaaagaatt ccttgacaga agaagagaga gcctttacaa agaggcggaa gaacagatag
    67321 tggatcttgt tctgagtatc tcaaagaaag tattaggcca ggaattatca cagaataaag
    67381 aaacgcttct gtccctggtt aatcaagcgc tgcaaaaatg cacatttaaa aacaagcttg
    67441 tcttgaaaat atcaccttct gattttgaat tcgttaaaga aaacaaatat cggatatgca
    67501 agatggtaga gggcatatcc gatattgata tagtttccga tttatcactt ccgaaaggaa
    67561 gctgcattgt tgaaacacct tccggtgaga ttaattcaag tattgaagtt caatttaatg
    67621 aaataaaaaa aatatttaca tatttgttga gaaatgagtg atcagcaatg atgggcattg
    67681 acatggaaaa gtataataag gctttgttaa gcaacgaact tataaagtac acggggcggg
    67741 tttcccaggt tatcggtttg accatcgaat ctcacgggcc cgcagtcaac cttggggaga
    67801 tttgcaacat ataccctttg aagggaaaca ccccaataaa agcggaggtt gtcggtttta
    67861 aggaaagcat agtgtgcctg atgcctttgg gcagcatgaa aggcgtaggc cccggaagca
    67921 aaattgaagc tacgggagaa agcctctcgg tgggagtatc caatgatatg ctcggcagag
    67981 taattgatgg gttgggaaga gtaatcgacg gaaagggcac tattaacaac tgcactatgt
    68041 atcctgtaga aaactcccct cctaatcctc tttcaagaaa aagaattact gagatattac
    68101 ctgtaggagt gagagctatt gacgggccgc ttacattggg aaaagggcag agaatcggga
    68161 ttttttcagg cagcggagtc ggcaagagta cattaatggg aatggtagcc aggaatacaa
    68221 aggcggatat aaatgttata ggccttatag gagaacgagg cagggaagtc agggaattca
    68281 ttgaaaacga ccttaaagag gatgggctgt cacgttctgt agtggttgtt gcaacatcag
    68341 accagcctgc actaataagg ctgaaagctg cacagctggc tacttccgta gctgagtatt
    68401 tcagagatca gggcaaggat gtgatgttaa tgatggattc attaacacgt tttgccatgg
    68461 ctcaaagaga agtggggctt gcaataggtg aaccgcctgt tacaagaggc tacactcctt
    68521 cggtgttctc cgtgatgccg agactgctgg aaagatccgg aacatcggat aaaggcagca
    68581 ttaccggact gtatacggtt cttgttgacg gagatgacat gaacgaacca attaccgata
    68641 cagtaagaag catattggat gggcatatag ttctgtcaag aagcctggca aataaaaatc
    68701 actacccagc aatcgaggta ctggcaagtg tcagcagggt aatgcccaat attgttgatg
    68761 atgagcataa ggaaaatgca aatgaattca agaacatcat gtcggtatat aaagacgcgg
    68821 aagacctgat aaacataggt gcatatgtaa aaggaagcag cagcagtata gacaggtcaa
    68881 tagaaaaaat aggagcaatt gaaagattcc tcaagcaagg cgttgatgaa aagacagaaa
    68941 tggaagagat tttgaacgaa atgagaatta tagtgtcgta ggagaagcgt atggctgtat
    69001 ataagttcag actgcaaaag cttttggatt acaaaacaaa agtcgaagaa gagaaaaaaa
    69061 acaagctggg ctccgctgta aagaggcttg aaaaagaaaa gcacaagatg gcggagttga
    69121 aaaaggaatt cagtgatatg caaagcttgt ttcaggagaa aacttctcaa ggaatggcag
    69181 taaatgagct aaagatattg gctaattata tcgattatta taagagggga ataaaagcac
    69241 aaaaagtcag gattaaaatg gctgaggatt atctttctgc ctgcagggaa gagcttatag
    69301 cagcgacccg ggaaaaaagg atgatagaaa agcttaaaga aatggattac agcaagtatt
    69361 tatatgaaga acaaaaaaag gaagaaaagc tggtagacga cctggtttca tttaaagaaa
    69421 gcaacaaatc ttaggaggaa aggctaatga atcaaccatc aaacaccaat gaaaaaaaac
    69481 ccgcagcaga aaaaagtaaa aaggctgatt caaagcgcgg taaaactaga gaaaagaagg
    69541 caaatacact gcttctactg attatagtaa tatttttgtc cgccatagtg gcttttggag
    69601 ctatattcat gtttaatttt gcagggctca gggcggaaac ttctgtgtgg ataagcaatc
    69661 tgccggttgt aggcaaactg ttgcagccgg tgttacaaaa aaagaatcct gagcagattg
    69721 aaagagaggt aattgaaagg gaaaagtcaa acattgcgat acagcagaaa gaattgaata
    69781 atctggagaa agagctttta agccgcgaaa aggaaatcga aaaaaaagaa aaagaattag
    69841 cggataagga attggaaata agcaaattac atgagcaatt gagcgaaaag ctccggagca
    69901 ttcaagagca ggtacagtac ctggaaaaag tagataacca aaaagccatg cagataatat
    69961 tgaatatgaa tgacaaggct tctgcagtac agattctcag aaacatgaaa aaagaaaaag
    70021 ctgcctctat tcttgcgcag atggatccgc tgcaggcagc acagattctt gaggatcttg
    70081 cagattgaaa ggaggtgaga tagtgacgtt cagcattgga ccaatgcccg catttaatgt
    70141 cagcaaaccg gataacagca gcaatgcagc atttttccaa ggccggcctg aaaatgagtt
    70201 cagcagcttc agagagattt ttaggaatac attaaaatca ggagacagat atgataccaa
    70261 aacagcagca gaagaagata caaaaatgga acttgccggc agggcggaaa taagacataa
    70321 gaaaagcagc aacagggatc cggaacctgt aaatgtgggg gatgaaaatc cggcagcaga
    70381 aaaagaagta agattcaagg atgtgattgc caatgatgta attgccgagg gtgcaaatgt
    70441 tgaaaaggca gttgctgatg atgaagctgt taaggaagca attgtcaagg aactgctgat
    70501 gaagcttgaa aaattgaata aaatgaagga tggagaaaac acagacgcag aaaagcaggc
    70561 acttcttgaa ggtattaaag aactgctgca ggaactggcg gaaatccgaa ccgaaatttc
    70621 cggagaggaa gctgcaggta tctgtgaaat ggtgtcagag cttgagaatg ttccttcaaa
    70681 gttacgggat gtaataatag caatatttga aaagcctgcc ggaacgaaag ctgcagtatc
    70741 ggctgaggta caaaatgaca tcattccgac agaagaatta aattcagaaa aaaatccggt
    70801 taaagtacct acgccagaaa aagcggatgc aaaggataac cttggggatg agagcagcaa
    70861 gatgccggcc cctgatgaaa gccaaaagaa tgatgtaaaa acagtgtcct caagcaaacc
    70921 ttcagttccg gaacaaaaag gcgacaaggc ggagacagac gcaaaggaag aaaacataaa
    70981 agcagctatg gatgataagg ttgagaaggt gtctgtagaa gaaaatccgg agaaggttca
    71041 ggaaacgggt aaaaacaata accgcaagga cgctgcaaaa gaaacccatg atacgattaa
    71101 tacaacccat aataagttgg atatggaggg aactgctgtc aggcaggaac aaaatattat
    71161 tttcagcaaa gccgagacag ttcagaatca ggctgaaatg ccaaaggcac aaccggtaag
    71221 caaggttgag attatcaacc aaatagtaaa aaaggcggaa gtaatactta ccggtacaca
    71281 gtcagagatg aaaatgcagc tggaacctga aaatcttgga aagcttacat taagaattgc
    71341 tgtggaaaga ggcgtgatta cagcaaagtt tacagcggaa agctacgagg ttaagcaagt
    71401 aattgagtcg aactttaatg agcttaggga catgcttcag gataagggac tggacataca
    71461 gaacttaagt gtttctgtcg gccatgaaag caaggaattt aacttcagta agaattatga
    71521 gacatggagg gacaagctta agtcaggggc ccaaaatatg aggcatgaca cgtatggcgg
    71581 atatcaggat gaaatcggaa caacagcggc aatcaaccca tacagtatcc acacagggag
    71641 atttgatcaa agagcatagg aggtgaaaaa atgccggtaa gcggtataaa cggccagtac
    71701 actgcatatg aaccgaacaa accgaaggaa tacaacagta accttgacaa ggatgcgttt
    71761 ctgaaaatac tggtaacaca gctaagatac cagaaccccc ttgaaccaac caaggataat
    71821 gagttcatag gacagatggc acagttcagc tcattggagc aatcacagaa ttccaacaag
    71881 gcaataagga tgaattcagc caacaatatg gttaacaagc tgattaaggc taactacaga
    71941 gctgaagatt ccacagagac aaaagagttg ataggcctgg tggaaaaggt tatgttaaag
    72001 gacaacgaaa tatatcttac aatggacgtg ctgggtacga aatacgatgt caagtttgac
    72061 gatgtcaggg aagttactga gcttgagaat tctgtagagc agatatacct tatgaatcag
    72121 accatgagaa gtaccacagc cttcaatctg ataggaaaga atgtcaaggg cacatatgaa
    72181 gagactgaaa aagtaaacgg tgttgagaga atcaagacag tggaaattga aggaactgtt
    72241 gagaaggtac gaaaggaagg aacatcaata tacctgacag taaacggaaa agagatatat
    72301 ttggaggata ttacagaagt aaactaagcc ttggaagagg tgatacgaat gagtgtaatt
    72361 tataacaaca aaccgataat tcccattggg aaacctatac agccgacaaa accttcagat
    72421 ggcagacaat ctgataagag cattagcttc gacgctgtat tgcagcagga gttatcgaga
    72481 caatcggaag tgaaattctc gaagcatgcg gcagaaagac tgcagatgag gaacatacat
    72541 cttacaaaag atgatttgaa caaactgaac gaagcagtga acaaagcggc tgagaagggt
    72601 gtaaaggaaa cactgataat aatgggcaat tcagctttca tagcaaatgt aaagaataaa
    72661 atgataatca cagctgctac ggaagacaat ctgaagaaca acgtgttcac aaatatcgac
    72721 ggtgcagtaa taatataagc cggacctttt taaggaggct tagcctttcg gaatgacaga
    72781 agaaaggcgt actaataatt aaggaggtca tttattatga tgagatccat gttttccggc
    72841 gtatcgggac tcagggcaca ccagctcaag atggatacga tcggcaacaa catagcaaat
    72901 gtaaatacag tcggatacaa atcacagagg gcaaccttcc aggaggtttt caaccagaca
    72961 ttaagagggg caggctcccc gcaagcaggc aagggaggca caaatccgca gcaggtaggc
    73021 cttggaatat cactttcctc aattgacacc tttcatgtaa gaggtgcggt gcagaggacg
    73081 gataacctga cagacctggc aataaacgga gacggatttt ttatactttc aaattcggaa
    73141 gacggattga gcagatccta tacaagagcc ggaaacttct cactggatga cgacggcaat
    73201 ctggtttctg ccaatggatt taaagtatta gggtatatgg tagacccgga tacaaatgtt
    73261 ttgaggagcc agttggaagg ggtaaagata tcaaagtccg agatggtagc agcaaaggaa
    73321 actacatatg caacctttga gggtaatctt gacaatcaga ttgcagtcgc accaggagca
    73381 gatgcggatt acgttttcac agcgggtgga gatgagaata ccccagatac cttgacaatt
    73441 ccagcagata caaaatactg ggagacaacc tcgacagtat acgatacact tggagggaaa
    73501 catacactta aatttacatt tatcagaggc atggaagcag atggtgcagt aggcggaggc
    73561 ccttcgcaat ggcaggtatt tatcaaggat caggacttag gcaaatacta taatgagagt
    73621 ggtggatttg ctgcttcaga cactttagag actattactc atggacttac ctttaagcct
    73681 gatggaacgt tagataccac aagcactaat ggaattgttg agcttacagt tccaggtaag
    73741 aatggctcag gtgatattga agatttgaaa atagacttta caaaactaac ccagtttgcc
    73801 aatgaatcca atgcagcgat gacaggttct gatggatacc cccaaggttc cttagacact
    73861 ttcagcatag ggcctactgg ggagatcaac ggtatattca caaacggcat gagcaagata
    73921 atcgggcaga tagcactggc agcattcaag aacccggcag gccttgagaa gacagcggag
    73981 aacatgttcc aagtaacacc caactcaggg gatccgatta ttgggcttcc cggcttcagc
    74041 ggacttggat cacttaactc gggcaccttg gaaatgtcga atgtagatat atccaacgaa
    74101 tttgccgaga tgatcagcac tcaaagaggc ttccaggcta attcaagaat aataacaacc
    74161 tcggatgaaa tgctgcagga acttgttaac ttgaagagat aatgtttatg caaatccctg
    74221 ggcctagtgc ccggggattt gtaaaatgat gaaaggaggc agttttatga taaaggttac
    74281 aagactcaac ggaattgtat attatctgaa ttgcgaacac attgaaactt tagaaaacac
    74341 tcctgatact gttattactt taagagatgg taaaaaattg ttggtttctg aaagtccgga
    74401 ggaaattatt gaaaaaatta tcgaatataa aaagaggatt tttgaccttt catcgctaat
    74461 tattaaagat aacttctaaa gtgaggtgca aataattgga tttagcttca gtatttggtc
    74521 tggtaggcgg aatagctgtt attcttataa ccatactcat cagcggtgat ctgattggat
    74581 atgtggatat accttcaata atctgtactt ttggcggaac aattgctatg acatttatgg
    74641 catttccgat taagaagctt aaagaaggta ttgccgcttt aaagtatgta tttgtatatg
    74701 cggagacaga cgctgagtat ataataaaat caataattga ccttgcaaat actgcgcgaa
    74761 aggaaggact gcttgctttg gaggaggctg cacagcagct taaagatgac tttctgcaga
    74821 aggggatact gctgattgta gacggtaccg accctgaact tgtaagaaac attcttgaaa
    74881 cagagctgtc ttttattgaa gaaagacata agagcaatca aggtgtatat gagtttatgg
    74941 ctgctatagg cccgggtttt ggaatgctgg gaacacttat cggcttgata aacatgcttg
    75001 caaacctgtc ggatccttca acagttggtc ccaacatggc agtggcaatt gtaactacat
    75061 tatatggttc aattattgca aacgtattct gtaatcctgt aataagcaag cttaagatac
    75121 gcagttctca ggaatctctt atgaaagagg taatgctgga gggaatgctg tcgatacagg
    75181 caggcgagaa tccgagaata attgaagaga agcttaaagc ctttatgtcg ccaaaactta
    75241 ggaacatgct tgattcagca aaggaaccaa aagagggaga ggaataagta tggccagaaa
    75301 aagagttcaa gatgagccaa gtcccggagc acctgcgtgg atgtctactt acggagatat
    75361 ggtaaccctt cttttgtgtt tttttgtatt acttttttca tttgcaactc ttgatgttca
    75421 gaaatttgaa gctataaaac aatctatgag cggttctctc ggtgtattag actcaggaat
    75481 gaccttgagc atggaacccc ttgctaatgc ctttccggct gattctccta cggaagaagc
    75541 ggaagaattc aagaagctct atgagcaaat gagtgaatat attaaggaaa ataatctgga
    75601 agcgagtatt actcttcgcc tggatgaacg gggactgctg gtgagattta tggataatgt
    75661 tcttttcgac tccggcaagg cagacttaac tcccaatgcc agtgatatta tcaataaagt
    75721 ggctgagata atcaggcaga acgacaaaat tatcagaatt gaaggacata ccgacaatgt
    75781 accgattaat accttcagat ttccgtcaaa ttgggagctt tcgactacca gggcagtaaa
    75841 tgtggttaaa tatcttatag aggaaaatgg gatatatgcg tacagattgt ccgcatccgg
    75901 ttattctgat cagcatcccg ttgatgacaa taatacggct gaaggccggc aaaaaaacag
    75961 aagagtcgac atggtgatac tcagagacga tattaaggat ttaaatccat aattcttggg
    76021 agggagacgg aatgaaacga aatattatcc taatagcggc tattgtgctt ttacttttgg
    76081 ttatagtatc aggtataata atatatatgt tttttaatag gccaaaagta gcagaagagg
    76141 aacctataaa aatcgacaaa aaactcataa tgttgtcatt gggtgattct tttgtgaata
    76201 atgtaaaaga cagcaaaaaa atatgcaaat tgacatttaa actggaggtc gacaaagaag
    76261 ttgccgaatt ggttacctac agggagtcag aaatcagaga cagaataaat gcacttataa
    76321 ggtccaagac tgaagcagat cttgaaggaa aagaagggca ggaaaagctt caaaaggaaa
    76381 ttgttgaaat tataaaaaag attattaatt caaagaaaat aaaaaatgtg tattttgacg
    76441 agtttatagt gcagtagaga aggaggtggc tttgtgtccg aggttctttc gcagaatgaa
    76501 atagatgcgc ttcttagtgc actgaattct ggagaaatag atgttcaaga gataaaagaa
    76561 gaaaaagaag aaagaaaagt cagggactat aacttcaaga taccgaacaa gtttgcaaag
    76621 gaccacacaa gaactcttca aatcatgcac gagaattatt caagactgtt gcagacatat
    76681 ctgtcggggt atcttagggc attagttcaa atagaagtta tttcggttga tcagctgaca
    76741 tataatgaat tcaccaactc tatgccaaac ccgtcagtgc ttggcattgt ggaatttgcc
    76801 ccacttaccg gatcaataat agttgaaatg acaccgacga tttcctttgc catgattgag
    76861 aggattcttg gaggtgcggg gcatgccttc gaaagagtga gaggttttac cgaaattgag
    76921 cttagcctcc tggaaaaaat aatcggacag attatcaact tttttaaaga cccatggaaa
    76981 aatgtaatag aattgaggcc aagacttaag aaaatagaga caaacccgca gtttgcacaa
    77041 attctgtcac ctaatgaaac agtagcgctt ataacattaa ataccaaaat cggaagtatt
    77101 gaaggaatga ttcatatttg tattccgcat ctggttattg aaccggtaat tccaaagcta
    77161 agcaccaagt tttggttctc aggcattacc aaggaaataa caggcagtga tttaaagata
    77221 atcgaaaaga aaatacagag cactctgctt cccataagag ttgtactggg ggacagcgag
    77281 attaccgtaa gagacttcct ggagcttgga gcgggtgatg taatacagct taatacatca
    77341 gtaaatgatg acctgaaaat atatgtaggc aatttaaaga aattcagcgg taagcccgga
    77401 ttgaagaaga acaaggtgtc agtgaaaatt acgcgaatag aaaagaaagg agatgattat
    77461 gatgagtgag atgctgtcac aggaagagat agacgccctg cttaagggag atagcgcaac
    77521 agcgcctcaa gataattcat cttctcaagc cggtcatgat agtgttctta caacggaaga
    77581 aatagatgct ctgggtgaga taggaaatat cagcatgggg a
//
LOCUS       DAOOAB010000006        75014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_405_length_75014_cov_13.756213, whole genome shotgun sequence.
ACCESSION   DAOOAB010000006 DAOOAB010000000
VERSION     DAOOAB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 75014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75014
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_75014_cov_13.756213"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1597)
                     /locus_tag="PKU88_02375"
     CDS             complement(155..1597)
                     /locus_tag="PKU88_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPX68161.1"
                     /translation="MLKRSDFEKIADSIKSKNLDITERSIPFHHGMVKLFYIMQLMDR
                     AALVESVVKPLILHCSSAQEPINAQMTIDTIIFADDCRLESDISKIEEYILSGMAVAL
                     FSTDEEYIVINIKKIEHRMIPTPQLTYTIRGPQDCFTENIDVNLSLIRYRLKDKNIQI
                     KKSEVGVRSKTIVAVIYIEDIANKTVVTEIQKRIESIDVDGIGESGELQSFLLNSKMQ
                     LFPNIGLIERSDMAFHSLIEGKVLVLVDGSGIALLAPKTFSEFFYSCDDRYDNKFFGL
                     FMRLIRYAAIIFAFTVSSIYVAITSFHTDILPSDYAISLAEMRVNVPFNALMGALTIE
                     FISELLREALLRVPKQIGPAVGIVGAIVIGQAAIAAEIFSPLLLIIASTSLLASFAIP
                     DYTLINPFRILKFILLLFTGMLGFYGFTLFLTFILAQLVSLNSFGVPYLAPWAPFNFY
                     DFKRSLIYSLTMDPKRPQYLSTKDRTRTKR"
     gene            complement(1611..2756)
                     /locus_tag="PKU88_02380"
     CDS             complement(1611..2756)
                     /locus_tag="PKU88_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPX68162.1"
                     /translation="MKKSGKIFLILVFTLCIPAFAAACADSKDIHEQSILMSICLDKK
                     DDEIYYYAEIANITGSNKDEGKSSSADRKYIFYYSHGKTIHEARRKLYMQLERRAYYG
                     AIRTLILTENFAKEHLVEYLYRLRTDELYRKKTMIVITTVDPEKLFKINNENDISVGF
                     SIEEMLETLDEEGQSFTRTTSRMLENLSSSYTGFLIPCIGLRNNKNSLVGYSVVNGTT
                     INGFIPVEDSKALVFLKTDKPRFFYTVPYKGNEFTIETSLKKRKIEPSYEDGNVSFDL
                     KFDFEAKLLYGDKKTPYNFDDISNEGVTKILTEILKNEIKDAVLEAQKHFKCDYLQFD
                     DEFRVKFPEEFESMDWQNEFPEASVNIDVKVVLSGSWMMDFETDVRK"
     gene            complement(2768..3850)
                     /locus_tag="PKU88_02385"
     CDS             complement(2768..3850)
                     /locus_tag="PKU88_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPX68163.1"
                     /translation="MANSITNRQIFFLLVMTLTTYTTIFLPKAMAETAGRSSMVPIMC
                     SSVIFGVVSVIITKLNNTFPGKVFFDYSQEIAGRFFSYLIASYFMLYFIFVGVFLKLT
                     FVNLMTSNFLPRTPRYEILLFGILLFAYAANKGVTNIARMFEIYGILFVLVTVVTCVF
                     MLTEGTKFNVLPFFAPSDIEGYTEAMKELIVPFGGIEVLLIVPFTEKNKEAPKIAFLT
                     LLFIGLFYVLIVESTIMILGINNTILLNDAFIEAIKITEAPVIERLDILYLTFGLSSL
                     FCGMIIVFTAALEFACRIFSRVKRHIVVVAIGVILYILCLLALNIKDIEKLLEFFSFY
                     LVIISSFLIPAAAFILAKVKKRTNIS"
     gene            complement(4052..5407)
                     /locus_tag="PKU88_02390"
     CDS             complement(4052..5407)
                     /locus_tag="PKU88_02390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPX68164.1"
                     /translation="MKISIIGTGYVGLVCGACLAKKGHDVICVDSQREIADRINNSEA
                     VIFEPYLSEYIGEAVAEGRLLATTDIEYGVASSEISIVCVDTPYKNGSIDLNNVEKAV
                     RDIGRILRIKNPYHVICIKSTVIPSATDTLVKNILEEESGKKAGEFGLVMNPEFLREG
                     EAVTDFMNPDRIVIGAYDDRSYEHFKMVYEGYFEAPLLRVSLRTAELIKYASNSLLAM
                     LISYSNEIAAISEAIGGLDAEEVLYGVTLDKRISPQIDGKFIRPEIVKYLKSGRGYGG
                     SCFPKDLRALIAFTESLGYAPKLLRATAEINEMQVGKITDRLEKELNGLEGKKILVLG
                     LAFKQNSDDIRESPAIRLVKLLSEKEAVIYAADPMATEKARKALEGYRGICFLEGYKK
                     TAISFDAAVLMTPWQEYLEFLNEGSSASLKTALLFDGCRALDKYKFNGSGIKYMGVGI
                     S"
     gene            complement(5418..6257)
                     /locus_tag="PKU88_02395"
     CDS             complement(5418..6257)
                     /locus_tag="PKU88_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotide-diphospho-sugar transferase"
                     /protein_id="HPX68165.1"
                     /translation="MFCTIVTKHRLHQCIAMYNSLKGRIPEAFLAVLAVDRESGRVLD
                     ALKLPGMLVISAEEQENDELRELKGQRSPSEYCWTLKPVLLLYLYERFADVKIFVYAD
                     SDIFFFDNPMKLFRRKDRWHVLLTTHKVNRKTNGGFAAFKRSNDSYKALKWWKDRCIE
                     WCYCKYDNGRFADQGYLDSMSGMFNGVEYIDIPGANAASWNYFKYDFSEMDGNILVNG
                     SRLIFYHFSGLRLKRYADTVALCGAEIPCVVCGIYSKALRDAIEKIEGIDRKVTDYFY
                     MGK"
     gene            complement(6268..7032)
                     /locus_tag="PKU88_02400"
     CDS             complement(6268..7032)
                     /locus_tag="PKU88_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPX68166.1"
                     /translation="MKTVILCGGYGMRMREVTEDIPKPLAIIGGRPILWHIMKIYSRY
                     GFNDFILPLGYKGEKIKEYFMNYDWMQNDFILDFSGNENKVYLLKKPEKWKITFIDTG
                     ADAMTGDRIKRIQPFIDEDTFLMTYGDGLCDVDIAKLLKYHQEMGVTATLTAVEKKSQ
                     YGILKARDGLAASFGEKTEIEGLINGGFFVLGKAIFDYLDHEGCSFEKDVLPRLAAES
                     RLAVFEHRGFWQAADTSKDLQDMIENWEAISNKLGG"
     gene            complement(7075..7479)
                     /locus_tag="PKU88_02405"
     CDS             complement(7075..7479)
                     /locus_tag="PKU88_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HPX68167.1"
                     /translation="MKSRYEVITISPYKDGRGELKKVFTMDMLGADESVEEIYVLYTK
                     GNSVRGNHYHKKNVEFFSVIKGTATIALRDLETDSTDVFRASSKDNIVIKVPENTAHC
                     FRNDEEEELVILAAATRQYEPGDTDIYPLRLL"
     gene            complement(7499..8563)
                     /locus_tag="PKU88_02410"
     CDS             complement(7499..8563)
                     /locus_tag="PKU88_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPX68168.1"
                     /translation="MSINKIIEEDVRDIYLEIGGTLKVIEGSSFLISGGVGFIGSYFM
                     DLLKFCNDNIFMESVTAICVDNFVTGVPERIGHIRHNENFMFLNADISKPIEIDYDID
                     YIINAASIASPSYYRKYPIETLETNVLGLKNLIELGMKKNIKSFLNLSSSEIYGDPDE
                     SHIPTGEDYNGNVSCTGPRACYDESKRLGETMVVNYYKEYKVPIKSARPFNVYGPGLM
                     LNDKRVIPDFFNDAIHKRKIQILSDGTPTRSFCYISDAVKGFMLVLLSEFDGEAFNIG
                     NDRTEISMKGLAELIAGIVGNVAIEYKKSKEKDYLTDNPQRRCPDLSKAKSYLNYYPK
                     VELEAGLGRLYHWYKQESNI"
     gene            complement(8567..9535)
                     /locus_tag="PKU88_02415"
     CDS             complement(8567..9535)
                     /locus_tag="PKU88_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX68169.1"
                     /translation="MEAGLLRNKKVLVTGSNGFIGSHTVSRLVKYGCEVFAAARDASD
                     LWRLDEVKDSIRMIAIDIRDNEAVRLCMARIKPEYVFHMAAYGVDSRQKDYVAAAETN
                     IMGTLNIVMAAGLAGCEKLLNTGTSMQYGNKKGAIKEEEYYSPTNIYGSTKGAATIIA
                     HQAAAGYNLDIVTIIPFGVFGEKEGSHKFFPHVILSALEDGEVSLSPCEQYRDYCYIE
                     NIIDGFMMAAVNKEVRNEIFNIGSGSIFKLKHYVNLIMKRFETGKRVCYGAVEYRKND
                     LWSPQPDLTKIRTRLGWEPRVNLEEGIDRTVEWFRKNHDKYSVKGR"
     gene            complement(9551..11404)
                     /locus_tag="PKU88_02420"
     CDS             complement(9551..11404)
                     /locus_tag="PKU88_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68170.1"
                     /translation="MIHYYCSIASSDYKYKVLALYNSMVKHDKEFNFFIICMGDEALA
                     SFAALKLENTVLIPVSAIESYYPELTKARKDRNDKEYAWTVKPSAFLYIFNNYNYIDH
                     IMWLDGDTMFLSDPGPIYLEWGRHSILLTEERYTGKHAYLSRIYGIYNTGLLGFRRDA
                     NSFECLKWFQSKVNEWCYDRMENGLWSDQMYVNDWPERFKGVAVIKNAGINMTPFILW
                     RFTAEEKRHIDARRLVLFHYYGFRYAGTDDYDLCTYKDWGFPEAVVRSIYMPYISACE
                     AALLQLKRGNAEETEQISETGAAEDKHNSQYCFCTLTTLEYLPKCLALLDSIESNTDS
                     FHIWICCMEDAAYDILTGMGLENVTLVRVSEIEDNKLRAVKKNRKDYEYCWTLKAPFM
                     LHIFEKYENVASLLYLDADIYLFSRPDRCFKALERHTVLLTHHNFSQPYKHLYRKKGR
                     FNAGIIGFKRCGTALSYLKWWRKKCIEWCFDVVSEGRFADQKYLENFLKPYGSAYLAE
                     NIGMNAAVWNLEDKEVHCRGEDIFIGGDLLVFYHFSSFMILGENEFDLWKWDSLKVSS
                     SAKELIYFPYARAVAESIKKIKPYINEISKVYSGMNAWYRASNSAIIENMD"
     gene            complement(11717..12481)
                     /locus_tag="PKU88_02425"
     CDS             complement(11717..12481)
                     /locus_tag="PKU88_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68171.1"
                     /translation="MTVPNASLPTVLCAGTTRLEVKTGGSTECKDLFFECLKVKKVFD
                     EFVLRDCVEGIKFKVCGNPHGDIIKPALILKNCKLSNVEIKDVSTNSDKRLKFSAKCC
                     CDVFGKDAKGNIIPLEVLDVPEGTNLSEGPCGELCFKFSVRRSYPNVTEENFERLLHF
                     LDQGRFELQCLGEAVIDEENNTTTGEILVTNLGAFIAVKFDAEVQLCVPVLGYCQIEE
                     DVAPIDVDFCEEFEMEDIPSFNPPQLGVPIPPYPIS"
     gene            12818..14341
                     /locus_tag="PKU88_02430"
     CDS             12818..14341
                     /locus_tag="PKU88_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68172.1"
                     /translation="MIDINPSAILEDSKGNRYFFFYRDSSIYYREVPIKGDIKDTMLI
                     SQANADFAAVIDADDSIYLTCNSRYKGVLLFIYTNSGWKFEPVVNLHNSSNIFIMDII
                     VQNGSIHIFFSKKLPIANMYNVYHINKNINDKTPYIEYSWRKNSLSEIYSQNIESSYS
                     LLPLRNGIIHYASVWYDGTLYYINYYCYDDSTKSWLHKSLSISYKNQAFIKLIHQNKK
                     INLLCYSNENEGSNIHHFLSKSSSSSEIDFRELGNVRIDTNGMIPLFYSDDKAIQMAW
                     IKDHVFHQYTFDDSAGRWRKAVDLPVTADTSINAVKIMKSLAPLSITKGFFILDKNYN
                     ISRPAEHISRSIPEDKPKEKAQAASIPDMNDYLRQILDEIRDLSDNVKYLNSRIGSSD
                     NRNSKDPTPPKSGTVDKTATEQPTLKKSGFKEKFMKNERLPNYESLLMKQENITTYVG
                     KPGTVNPASDISKDSEKQAFASPNTAAPNNKENWTEDPEEIPYKYNSLMKKIGEFFK"
     gene            complement(14356..15186)
                     /gene="dapF"
                     /locus_tag="PKU88_02435"
     CDS             complement(14356..15186)
                     /gene="dapF"
                     /locus_tag="PKU88_02435"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPX68173.1"
                     /translation="MKFYKYTGLGNDFILINNLRSNIHIDGSLARKMCDRYFGIGADG
                     IVLASPSESCDIRMEIFNSDGSMAEMCGNASRCFAKFCYEKKLVDKESFTVETLAGTI
                     SPRLNIEDGAVKEVTVDMGMPKFGSKDIPFNADLDKVVDYPIRVDGKEYLITSMFMGV
                     PHTVVFTDSIEDEKVISEGRKIEVSDYFPRNTNVNFVSIINRNEIILRTWERGAGYTM
                     ACGTGACASVVAGIASGKLGSIVRVHLRGGDLTVEWKAGEHVQMTGEAKEVFTGEYEI
                     "
     gene            complement(15233..16498)
                     /locus_tag="PKU88_02440"
     CDS             complement(15233..16498)
                     /locus_tag="PKU88_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPX68174.1"
                     /translation="MSTRAQTVWEAYRFPIILFTAIIIGSIIGIAMGADAAVFKPLGD
                     IFLNLMFTVVVPLVFITISSAVANMANMRRLGSILKNLLLVFVVTGIISAIIMIFFVT
                     VIPPAQGVNLNVNAEEPVEKISLANQAVKAITVSDFPELLSRRNMLPLIVFSVLFGFC
                     VTALGEKGKKVAEALDTLSNVMMKMVSFIMYYAPIGLAAYFAALVGEFGPELLKDYAR
                     AMAIYYPVTIAYFFIAFFVYAYYSGGMPAVKRFFKNIVSPAFTSLATQSSIATLPVNL
                     ESARKMGVPKDIREIVLPMGATMHMDGSCLSAILKISFLFGLYGKEFTGIGTFATAIL
                     ISVMSGVVMSGVPGGGLIGEMLIVNLYGFPPEAFPIIATIGFLVDPPATMVNACGDSV
                     AAMMVTRLVEGKEWLAKVIKGEAQGQFTE"
     gene            complement(16543..17796)
                     /gene="lysA"
                     /locus_tag="PKU88_02445"
     CDS             complement(16543..17796)
                     /gene="lysA"
                     /locus_tag="PKU88_02445"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPX68175.1"
                     /translation="MNYYFSDCDTVALAKEYGTPLYVMSEDIIMERLNEIRYSFTEKY
                     QDVQAYYASKAFLTREMARIIKRENMGLDVVSGGELYTAKSVDFPMEKIMFHGNNKTP
                     EEIKTALECKIGRFVCDSIWEIQLIDRLAKEMGVKADILLRITPGVDSHTHKYILTGN
                     IDSKFGIPIPHVNEAVKLSLESEGITLKGLHFHIGSQILENESYIMAVGIMLNLIKKI
                     KDEVGYVAQEFNLGGGFGIKYLDDTEKRKLSYFVDPMMNSIEEFCSNNGIKRPRIFIE
                     PGRWIVGEAGITLYTIGSIKKIPGVRTYAGIDGGFPDNPRPALYQAKYDGIIANKADS
                     PKKERVTIAGKCCESGDILIWDLYVPELQPGDILAVKSTGAYGYSMASNYNRNPKPAV
                     VMLSKGKPRLIVKRQTYEDLLRNEC"
     gene            complement(18129..19466)
                     /locus_tag="PKU88_02450"
     CDS             complement(18129..19466)
                     /locus_tag="PKU88_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPX68176.1"
                     /translation="MKKDMYSFFDMNREQEMKKESPLALRMRPSDLSEFVGQEDIVAP
                     GKLLYRAIKADKLTSLIFFGPPGTGKTTLAKIIANTSKSHFEQLNAVTSGVGDIRRVV
                     EEAKNRKGMYGQRTILFIDEIHRFNKAQQDAMLPYVEDGTVILIGATTENPYYEVNDA
                     LISRSTIFRLNPLKAKDIRKLVERCLTDEEKGLGSYNIEITEEAMAHIVNIANGDARA
                     ALNAVELAALTTERDSDGKIHIDLKTAEDCIQKRAVNYEKKGENHYDTISAFIKSMRG
                     SDPDAVLHYLAKMLYAGEDPKFIARRIMICASEDVGNADPNAIIVATAAANAVHMIGM
                     PEAQIILSQAALYVACAPKSNSAVVGIGKAMKDVENKRTGAVPLHLRNDAFKGARDWG
                     YGVEYKYPHDYEGNFVEQQYLPDEIKDAQYYFPSDNGYESKIKDPKLKNRRIR"
     gene            complement(19574..20119)
                     /locus_tag="PKU88_02455"
     CDS             complement(19574..20119)
                     /locus_tag="PKU88_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPX68177.1"
                     /translation="MKILIFGVLLLLVLIAISTYNSLVVKRNRVKNSWAQIDVQLKKR
                     FDLVPNLVETVKGYAKHESETLEAVVKARNQYISAGTPEEMMKANTEMTGALGRLFAV
                     AESYPDLKANTNFMELQGELSELEDKIAYARQFYNDTVLMYNNAIQVVPANIIAGLCG
                     FREEPYFKADESERQNVKVQF"
     gene            complement(20166..22010)
                     /locus_tag="PKU88_02460"
     CDS             complement(20166..22010)
                     /locus_tag="PKU88_02460"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPX68178.1"
                     /translation="MRRRITILLIFLITCALLSVITAYCDDRSYYMSSFKINAQLDSM
                     GNMDIIEEITYEFDGSFRGVYRTLRTAGSDGIEAVEVYKKQPDMLSPFVQDNSERDNT
                     FQLIGEDGGIKLKIFSAAVNESRTFILKYRVLNAAAKFNDIAEIYWKFMGEDTEVKIE
                     NFELVIKIPEGADKEQIKVFGHGPLSGFSEIADSKTVVLRVDELPARNFVEARVLFPP
                     ELIKDSKKVFNRDALAEIMSEEKEFADEANEIRTKARIIVGFSFVYVLFELLMIVYLY
                     FKFDKEYKAKFAGDYFRELPGSYSPAVMAVLWNFGSVKPRDLTATLMDLVRMKYLELI
                     VEKEEVNGLFGSRADNEYIFKLNKEADLMVLSPHEKYAIEWLIFRIGDGERVSLEDIE
                     NSSKTRESAIDFSRDYDIWTGLVKSEADSYSFFDKNTVKGILFGVLTAVIGMIFGGYT
                     AARHDNILGFAILMLVSIILLIYSLTIRRRSKSGVEQFKMWKAFRKFLRHFSSLDKAD
                     LPAVIMWEHYLVYAISLGVAKEVISQLRLVFREEDFNNSHLTYLYYGRYGHIHNYFDT
                     IDNITDSMVKTTESVYRQAMSKASSGSGGGGGFSGGGGGGGGGGAGAF"
     gene            complement(22153..23214)
                     /gene="orr"
                     /locus_tag="PKU88_02465"
     CDS             complement(22153..23214)
                     /gene="orr"
                     /locus_tag="PKU88_02465"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HPX68179.1"
                     /translation="MSYPAIKVNKEKLKHNTKVLAEKCGRLGIDIVPVTKVYCGMPEI
                     AEASVAAGAKMLADSRIENIKKLKELNAEKMLLRIPMLSQVDEIVEHVDISLNSEYEV
                     IKALSDRAQKRGKVHKIILMADLGDLREGVWYKDAIKFAGRIVKLPGIKLVGLGTNLT
                     CYGAVIPSVENLGLLAALAEEIEKKYDIKLDIISGGNSSSLHLVEKNEVPKGINQLRL
                     GEAIVLGNETAYGERIEGTYGDVFTYMAEIIELKEKPSVPVGETGVDAFGEKPVFVDR
                     GIRKRAILASGRQDLKADGIQPRDKDAIVIGASSDHLIIDVSDCGREYKVGDIMEFDL
                     GYGALLAAFTSEYVEKIIE"
     gene            complement(23218..24573)
                     /locus_tag="PKU88_02470"
     CDS             complement(23218..24573)
                     /locus_tag="PKU88_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HPX68180.1"
                     /translation="MKISVLVAEIGSTTTVVNAFDNIMSPCPKFLGQGQAPTSVYNGD
                     VTIGLKGAVDDLKRNLGAPELEWNEFLATSSAAGGLRMTVHGLVYDMTVRAAKEAALG
                     AGAIIKHVTAGRLRRTDLKRLEDVRPNIILVAGGVDYGERDTALHNFEMIASMGLGIP
                     VIYAGNIENQEEVRLIAEETNTRLYIVENVYPKVDMLNVEPTRKVIQEVFEEHIIHAP
                     GMSTVRDMVRGPIIPTPGAVMEAARLLKEHLGDLVVFDVGGATTDVHSVTEGSEEISL
                     MLISPEPAAKRTVEGDLGVHVNIPHIVEKIGLENLKKEFPEAEELVNNAKPIPETERE
                     KAFVERLTKEAVLTSLERHAGKLRFLYGPTGKKTVAEGKDLTNVKYIIGTGGALTRLP
                     GRIDILKSVGLQGKGTELYPGKEAKVLIDNHYIMASLGVLSKRFPEAALALMKESLGV
                     N"
     gene            complement(24563..26767)
                     /gene="oraE"
                     /locus_tag="PKU88_02475"
     CDS             complement(24563..26767)
                     /gene="oraE"
                     /locus_tag="PKU88_02475"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HPX68181.1"
                     /translation="MKLDPNKKLDIKELLQDLESYRPRRRGWHWREGRNKNRKIGDFE
                     YHETSEGLKNYIPLPGSRGFNYIDPQPDCVVTTEIASGRFEDDIRRMRMAAWHGADHI
                     MVIRTMGQSHIDSLIEGTTQGIGGIAVTRKQCRATRKALDMIEDEVGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKVMAWADILQIDGA
                     HNANATAMKAWKVRPELMVQHAINSIFSRKVGIKPENIALSTVPPTAPPAPRMRIDLP
                     YAVALRDFFSEYKMRAQQNTKYMESDMREATVTHTLDLLISRLTSADIQSTITPDEGR
                     NVPWHYNNIAAVNTAKQALNGMDGLREMVKLDREGPLGKEVRELKERAVLFMEEILET
                     GGYFKAVENGFFIDSGYYPERNGDGIPRQIDGGIGVGMIFERDKDYFAPVSVHYGYNN
                     IPSEFKKASDAIGNDTFEDPSKIIYIDELDEYDNVNTRLADLEKYYNTNTVKPEVEWR
                     ADGVVATTIFLPLDERTAEFAAIKCGEKLGLEDVTVVHKQALHPSEGTLVEIKGKVIF
                     DINVNELVIPEKVQTLSEEEIREDISAKPMFVVAATIGNDEHSVGMKETLDIKHGGIE
                     GFGIKYLYLGTSCPVEKLVDAAIETNADAILASTIITHDDVHTKNMRKIHDLCVEKGI
                     RDKVMLVVGGTQVTNEIAVEAGMDAGFGRGTHGIDVASYLVKRRREKHSNEN"
     gene            complement(26782..27138)
                     /locus_tag="PKU88_02480"
     CDS             complement(26782..27138)
                     /locus_tag="PKU88_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HPX68182.1"
                     /translation="MAVKRADDYEIRRKHLAELSDEELKKRFWELAEKIVDPMLALGY
                     KNTTPAIERSILLRMGFSSLEVKPIVEGVMRKGLMGKGAGNVVWRLSKRLGVSVREAG
                     VGLAEGKYWDDVDALF"
     gene            complement(27153..28574)
                     /gene="ortB"
                     /locus_tag="PKU88_02485"
     CDS             complement(27153..28574)
                     /gene="ortB"
                     /locus_tag="PKU88_02485"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HPX68183.1"
                     /translation="MSKDMSYAAVMARKNEIMKQAIGIDYSVFESGGISFDYERMMRE
                     AGYTLEEMQQIQAETSVGNTPVVELKNLTALARKLAPKGKGARIFIKDEAANPSGSFK
                     ARRAATAIYHAKKHGYKGVVTATSGNYGAAVASQAAKRGLKCIVVQECYDSRMVGQPE
                     ILEKQRACEAYGAEVVQLTVGPELFYTFLTLLEETGYFNASLYTPFGIAGVETLGYEL
                     AMQFREKIGRDPDKVVITHAGGGNVTGTARGLLKAGAKDVEVVGASVDLSGLHMASDR
                     DFNRKSFTTGHTGFGIPFATWPDRADVPRNAARPLRYLDRYVLVKQGEVFYITEALSV
                     LEGLERGPAGNTSLTAAFALAQEMDKDQIIVVQETEYTGAGKHPLPQLTFAKQNGIEI
                     LFGNPDRDEPGKNIILPAHPSDIKVRDYDIDKARASYIKNCINNCKVTEITKEDFEFL
                     VRDAKTDDSFVEGVLAELNVKIK"
     gene            complement(28567..28878)
                     /gene="ortA"
                     /locus_tag="PKU88_02490"
     CDS             complement(28567..28878)
                     /gene="ortA"
                     /locus_tag="PKU88_02490"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HPX68184.1"
                     /translation="MISAKKGDWVQIRQVVLKPEERAPQVPEDTKKMPLELWVKGIAQ
                     EDAKIGSTVEIVTATGRKVRGELTAVNPRYIHDYGDFVPELLKVDMQLKEILFGGDSN
                     E"
     gene            complement(29136..30182)
                     /gene="ord"
                     /locus_tag="PKU88_02495"
     CDS             complement(29136..30182)
                     /gene="ord"
                     /locus_tag="PKU88_02495"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HPX68185.1"
                     /translation="MRKENVKVIIWGLGAMGGGMADMLLKKKGVEIVGAVGRGAKIGK
                     SMYDYIKTERGSNKDVIIGSYEDVIKENAADVVLICTDSFTKGVFDKIKYCLEKKINV
                     ISSAEQMAYPKAAEPELTKQLDEIAKANGVTVLGTGINPGLIMDLLVVAFTGACEEVE
                     HIVARRVNSLSPFGPAVMEEQGIGITVDEFKEGVRTGKLAGHVGFAESIGMIADAIGW
                     KLSDFNQSMEPIVTEVDRKSPYGFAKAGDVAGCAMKGYGYVDGELKIEMDHPQQIEPH
                     LGGVETGDYIIIKGTPNVNMANKPEIPGGIGTIAMCVNMIPHVINARAGLKTMIDLPV
                     PRAIMGDMREMIEE"
     gene            30587..31384
                     /locus_tag="PKU88_02500"
     CDS             30587..31384
                     /locus_tag="PKU88_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPX68186.1"
                     /translation="MYDFHVHSHFSSDSTASMQSMAAKAKCIGLKGICFTDHYDLEYQ
                     STEISFEFSYDRYFDEINSLKANSSENLEIYTGLELGMQPHLSAENEALLKGRSFDFI
                     IGSIHCVNKKDIYCRTFLEGITENEGIRNYFDDMLQCIEEFKNFDILGHLDAVRRYLY
                     NKDNSFSLDLYEESVRNVLSKLVHSNKGIEVNTAGLRYCLSSFHPLPDILKLYRLLGG
                     EIVTVGSDAHKPDDLGYGIKNALSVLSELGFKYYAIYKDRKPIFIKI"
     gene            complement(31576..32982)
                     /locus_tag="PKU88_02505"
     CDS             complement(31576..32982)
                     /locus_tag="PKU88_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX68187.1"
                     /translation="MSLIYKSIIDTILKNIDIGIHVVDSSGKTIIYNQAMADMEGLDI
                     NEVMGKSILDIFPSLTTETSTLLTVLKMGKPIYNKYQTYLNNKGKQISTLNSTIPIRI
                     SQGKIGALEIAKNITKEKELSDKLVYLHQELASKDIDGSSLKGYTFRNLIGEDEKFLN
                     AVEIARKTAMSSSTVLIYGETGTGKELFAQSIHNESSRKHKPLIAQNCAALPESLLEG
                     ILFGTVKGSFTGAINRPGLFEQANGGTLMLDEINSMGHQLQAKLLRVLQEGNIRRIGG
                     LSDIPVDVRILATTNEDPLVLLQKGILRKDLYYRLNVISVSIPPLRERKTDIMLLTDH
                     FIKKYNNILQKDIWYVSNEVKEAFLNYNWPGNVRELENIIESAINMVNRGHVIKPEHI
                     SQEMCEVMFKAGKNNHYLNLNEDQPLDRIVAEVEKGLILEALQKCENNISRSAEMLGL
                     KRQTLQHKLKKYDINIKE"
     gene            complement(33088..34329)
                     /gene="glyA"
                     /locus_tag="PKU88_02510"
     CDS             complement(33088..34329)
                     /gene="glyA"
                     /locus_tag="PKU88_02510"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPX68188.1"
                     /translation="MKIDRIKACDSEIAEAIIKELDRERNKIELIASENFVSPAVLEA
                     MGTVLTNKYAEGYPGKRYYGGCEYVDIVEDIARERMKALFGAEHANVQPHSGSSANQA
                     VYFTVLEPGDTVMGMDLSHGGHLTHGSPVNVSGKFFKFIHYGVSQNTEMIDYDNVRAI
                     AKECRPKLIVAGASAYSRRLDFKAFRDIADEVGALLMVDMAHIAGIIAAGLHQSPVPY
                     ADFATTTTHKTLRGPRGGAILCKQQYAKAIDKSVFPGMQGGPLMHVIAAKAVCFREAM
                     SEEFRGYIEQVLKNASVLADSLTAKDIDIVSKGTDNHMMLLDLRRKGITGRDAERLLD
                     DIGITTNKNTIPFDPQSPFITSGLRIGTPAVTTRGFKEDEMKEIAEIISAALSAPESK
                     AECISRVKALCGKHPLYPEFE"
     gene            complement(34476..34802)
                     /gene="trxA"
                     /locus_tag="PKU88_02515"
     CDS             complement(34476..34802)
                     /gene="trxA"
                     /locus_tag="PKU88_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPX68189.1"
                     /translation="MSKNIKIVNQGNFDSEVLKSNKPVLVDFWAAWCGPCRMVAPVID
                     QLADEYEGKIDIAKVNVDENPELAGRYDIMSIPSVFLFDKGSKVDGVIGARPKQAFDD
                     MLKKHI"
     gene            complement(34910..35350)
                     /locus_tag="PKU88_02520"
     CDS             complement(34910..35350)
                     /locus_tag="PKU88_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPX68190.1"
                     /translation="MREIGKVVKIEGNQATISFTRGTACGECGKCQVGKDKLEMMMTA
                     ENDVGAQVGDEVEIELENMNFMTAVTIAYGFPLLALTAGIFGGYYGSLALGLEDNSAQ
                     AIGVALGLAALAVSYLIIKFKDKSIKKMKKFKPVILSIRTKDSI"
     gene            complement(35369..35641)
                     /locus_tag="PKU88_02525"
     CDS             complement(35369..35641)
                     /locus_tag="PKU88_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPX68191.1"
                     /translation="MDNKEISKEDILNRMKKIEGQAKGIQKMIEEDKCCGDIMVQISA
                     IRSAINKVGGLIMDRYIKECLKESLKDENSDKSIEEIIETIVRYVK"
     gene            complement(35813..36562)
                     /locus_tag="PKU88_02530"
     CDS             complement(35813..36562)
                     /locus_tag="PKU88_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPX68192.1"
                     /translation="MNAFSRTELLIGKDGIEKLKKSTVAIFGIGGVGSYTAEALCRCG
                     IGRLVLVDYDNICLTNINRQIHATRKTVGLPKVEVMKERLTDINPEADIVVHKKLYNE
                     ETSEMLLLPEYDYVVDAIDMVSSKISLVMKCIEMNIPVISCMGAGNKLDPTKLVITDI
                     YKTSVCPLAKVMRYELRKRGVKSLKVVYSKEEPISPKKDVADCKTNCICSNKETANCT
                     KRRQIPGSISFVPSAAGLIIASAVARDILDL"
     gene            complement(36677..38446)
                     /gene="aspS"
                     /locus_tag="PKU88_02535"
     CDS             complement(36677..38446)
                     /gene="aspS"
                     /locus_tag="PKU88_02535"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPX68193.1"
                     /translation="MARINRTHMCGELNMDNLGEKVTLAGWVQRRRDLGGLIFIQLRD
                     RSGIIQCTVNKDATPDVFAEAFKVRSEYVLKISGEVIKRDENAINPKMPTGYIEIAVS
                     SLEILSRAETTPIYIEDDLNASEQLRLKYRYLDLRRPHMQKFLFTRHKIAKVTRDYMD
                     ENGFVEVETPILIRTSPEGARDYLVPSRVQPGNFFALPQSPQLFKQLLMVSGFDRYFQ
                     IAKCFRDEDLRADRQPEFTQIDMELSFVEKEDVMEVNEGLLKRLFKEVLDVDIQTPFK
                     RLTYKEAMEHYGSDKPDTRFGLKLKDIGDLVANSEFKVFTETRANGGIIRAINAEGLG
                     AKLSRKDIDSLGEFVKTYRAKGLAWINITSEGVKSPIAKFLKDYELNGIIDRVGGKLG
                     DIIFIVSDKPKVVFDSLGQLRLELAKRFGLIDNTKYDLLWVTEFPLLDYDEEENRYVA
                     MHHPFTSPMDEDLELLDTDPLKVRAKAYDVVLNGYELGGGSIRIHRQELQEKMFKLLG
                     FTQEQAWSKFGYLLEAFKYGTPPHGGLAFGLDRIVMLFTGTDNIRDVIAFPKTQTASC
                     LMTNAPSAADPKQLEELHIEVKI"
     gene            complement(38458..39714)
                     /gene="hisS"
                     /locus_tag="PKU88_02540"
     CDS             complement(38458..39714)
                     /gene="hisS"
                     /locus_tag="PKU88_02540"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPX68194.1"
                     /translation="MLTQAPKGTKDVLPSESYKWQYVEDLIREICALHGYKEMRTPIF
                     EHTELFERGIGDTTDVVQKEMYTMKDKGGRSITLKPEATAPFVRAYIEHSMFNDIQPV
                     KVFCTTACFRYEKPQSGRLREFHQFDIEAFGSSDPSVDAEIINIAMILYGRLGVKNLE
                     IRINSVGCPKCRAEYNKKLREYLSGKIDKLCETCKDRFHKNPMRVIDCKEESCKEQLA
                     DVPYMLDNVCSECGEHFEGLKRSLEALGLEYVIDPQIVRGLDYYTKTAFEIVSRDIGS
                     QGTVCGGGRYDGMIEECGGPSTPGIGFGLGLERLLLVVENQGIKIPEPVYMDVYIANI
                     GKEAAEEALRQVNLLRKEGIKAEKDYMGRSLKAQMKYANKINARYVAVLGEEEIKSGK
                     IKVKDMNKGSEEEISLDALAGYIKNM"
     gene            complement(39720..41213)
                     /gene="hemZ"
                     /locus_tag="PKU88_02545"
     CDS             complement(39720..41213)
                     /gene="hemZ"
                     /locus_tag="PKU88_02545"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HPX68195.1"
                     /translation="MIEIRLINHEYYNEAADIVRMYFGKCEIVRSEGALADSQSFLQD
                     TGILITSRLDINGEIAVCVSTYLEGQKHEEAVEKSGIRAADGNEAKRLVKKSMYKLLA
                     AVFKREYPWGVLTGIRPVKIVHELMNNAVSTTDIPERLAEEYLVSRDRAELAVRIADI
                     ERPFVYPYNNREISIYIGIPFCPSRCEYCSFTSNSIKAYRGYVEPYMEKLTEEIKRVS
                     EFLNTSGFKVQTIYIGGGTPTALNAQQLERLIKCIGNSFGRQECEFTCEAGRPDSITK
                     EKLQVLYENNIGRISINPQTMNDDTLMRIGRAHDPGQVIESFRLAKEMGFGNINMDII
                     LGLPGETMTELAHTLEEIKKLDPESVTVHTMALKRASVLNEKYMKSALPDDENVSRMM
                     EYTKGFLGDMGMHPYYLYRQKHMVQNLENIGFSKKGFECIYNMQIIEERQTIVAFGAD
                     AVTKVVINSENRLERQHNIKDIKLYIENIEAMIRKKLDVLEQILSEE"
     gene            complement(41222..41404)
                     /locus_tag="PKU88_02550"
     CDS             complement(41222..41404)
                     /locus_tag="PKU88_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68196.1"
                     /translation="MNKSSKKILASKHKNRMYQEYNELIDMIESGMEDFEIATELGIA
                     SGIVSEVKNQIYNDDI"
     gene            complement(41482..42102)
                     /locus_tag="PKU88_02555"
     CDS             complement(41482..42102)
                     /locus_tag="PKU88_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX68197.1"
                     /translation="MMKFKSLALGIYQTNCYIVYDEKTKETAVIDPGGDFPAIKSYIE
                     ANGLNIKYIIITHAHGDHIGALKELKEYSRALVCIHREDSEMLKSKTMNFSGMLGGAA
                     IEMTADKYLEDGEVLELGDTKLNIIHTPGHSRGGISIYCDKVLFSGDTLFKNSVGRTD
                     LYGGSMTELVSSIREKLFTLPDDTMVYPGHGPSTALLHEKQENSFV"
     gene            complement(42123..42572)
                     /gene="dtd"
                     /locus_tag="PKU88_02560"
     CDS             complement(42123..42572)
                     /gene="dtd"
                     /locus_tag="PKU88_02560"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPX68198.1"
                     /translation="MRAVIQRVNSGSVAVDDKIVGSIGKGFVVLLGVNEKDTEADVLY
                     MVDKTVNLRIFEDDNEKMNLSLLDIKGELLVISQFTLYGDCRKGRRPNFMSAARPEKA
                     EELYNEFVKKCRELGVKTETGTFQAYMKVNIENDGPVTVIVDSEKII"
     gene            complement(42597..44765)
                     /locus_tag="PKU88_02565"
     CDS             complement(42597..44765)
                     /locus_tag="PKU88_02565"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPX68199.1"
                     /translation="MLEDLLKIIKKYNPTGDAEIVERAYNYAARAHDGQKRVSGEDYI
                     FHPLEVAKILAELNMDNITIAASLLHDVIEDTKCTYEDCKNLFGEEIAMLVDGVTKLG
                     KLEYKTKEEQQAESLRKMFIAMAKDIRVIMIKLADRLHNMRTLRFLSPEKQKEKARET
                     MEIYAPIAHRLGISKVKWEMEDLALRYLEPKGYYELVDKVAKKRKEREDEINQVMEVL
                     KGKLEEVGIEAHIEGRPKSFYSIYRKMYYQNKTFEQIFDLTAVRIIVDTVKDCYGVLG
                     IVHTLWKPIPGRFKDYVAMPKPNMYQSLHTTLLGFGGQPFEIQIRTWEMHRTSEIGIA
                     AHWKYKEGKTSQSEFDEKLKWLRQMLEWQNEIKDTREFMETLKVDLFTDEVYVFTPKG
                     DVVDLPVESTPIDFAYKIHSQIGNRCIGAKINGRIVPLDYKLQNGDIVEVITSAVANG
                     PGRDWLKIVKSSQAKNKIRQWFKKEKREENIQKGKELLEKEIRRNGYTTVQLMRPEWV
                     EAIYKKFSLHSLEDMYASLGYGGLTPNQIIAKLKEEFRKTQKLEEITEDSIERQAEKA
                     QERKKRYNSTGVKVKGVENILIRFSKCCNPVPGDEIVGYITKGRGVSVHQKDCPNVAG
                     LLNEEERFIEVEWSSQTKESYSADVEIRAMDRKGLLAEVTTIINESKINISSFHCRIT
                     KDKMAIINFILDITDIEQLNKLIRKFRRIEGVVDVFRAKQ"
     gene            complement(44907..45419)
                     /locus_tag="PKU88_02570"
     CDS             complement(44907..45419)
                     /locus_tag="PKU88_02570"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPX68200.1"
                     /translation="MDLIKYIRTIENFPKEGISFKDVTTLLKEGDAFKYAVKSIADNL
                     SNYDFDIIVGPEARGFIFGAPAAYELGVGFVPIRKPGKLPAETACYKYELEYGMDALE
                     IHKDAIKKGTRVAVVDDLLATGGTVLSAVRLVEQLGGEVVHIAFLIELEFLKGRENLK
                     GYDVSSIIKY"
     gene            complement(45438..47138)
                     /gene="recJ"
                     /locus_tag="PKU88_02575"
     CDS             complement(45438..47138)
                     /gene="recJ"
                     /locus_tag="PKU88_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPX68201.1"
                     /translation="MNKRWTAYSRSMDSLNPMIEEFQLPEIIIRAMINRGIDTVPKAR
                     EFFNTSLCSLHDPYLLKGMEQAADRIFKAVRSNEKICIYGDYDVDGITSTAVMIKTLR
                     KLGADGVYYIPNRLEEGYGMNLNSIDRIKEMGVSLIVTVDCGIKSCEEVDYAKELGID
                     IIITDHHECGEELPDALSIINPHQPGCGYPFKDLAGVGVTFKLAVALLNRAGSPGFAE
                     ELLDIAAIGTIADVVPLLGENRIIVKNGLVSIQNTDSQGIKALLAVCGLKGKELNSYN
                     VAFMLAPRINAAGRIDDAGECVELLLTDNAEKAMETAAKLDSDNKMRQSIENGILNCA
                     EAMIGESVDLDRDKVLVLCREDWHIGVIGIVASRLADKFYLPAFIMVCDGEFCKGSAR
                     SIPGFNIFEAMSRHGYLFERYGGHEMAAGFTIRTEKIDELRERMNFEVENTLGEDKLI
                     PEIFVDYKLEPKDVTLDTAKLLKLMEPFGTGNTVPLYVFRELSVTSFKGVGNDSRHLS
                     LKVFDGEKEISCIGYNLGNMQKILQIGEKIDIICSIENNIWNGTESVQLNIKDIKLIN
                     "
     gene            complement(47176..47520)
                     /locus_tag="PKU88_02580"
     CDS             complement(47176..47520)
                     /locus_tag="PKU88_02580"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPX68202.1"
                     /translation="MQFTIVISLVFAVIIAFFAVQNAGVVVINFLWYKVSLSQAVVIL
                     CSALIGVLIMLPFDIIRRIKYKFKLRELTGENKKIKEELKRIESEKLPAKEPVMQEKP
                     QTIEDIDKMDIS"
     gene            complement(47891..48778)
                     /gene="secF"
                     /locus_tag="PKU88_02585"
     CDS             complement(47891..48778)
                     /gene="secF"
                     /locus_tag="PKU88_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPX68203.1"
                     /translation="MKTIDFMGPRKIWFGISLALLAVGLIFLIINGGLNLGMDFVGGS
                     SIHATIGKTFDVSEIREIMDKYDPEATITHAGQNKDQVIVRTKINFDNEKRAEIIKAF
                     SDKYSIESNNISFETIGPTVGKELAMQSLIAVMLAFAGIMIYVSFRFEWRFGLASLSA
                     LIHDTAMMVAAYAVFQIPVNSTFIAALLTIIGYSINDTIVIFDKIRENLKNEHSKKID
                     YDNLVNVSVTQTLARSINTSLTTLITIFALYFLGVPAIRDFAFPMIIGIVSGCYSTIF
                     IASPLWVILRRRFKVSRAR"
     gene            complement(48780..50045)
                     /gene="secD"
                     /locus_tag="PKU88_02590"
     CDS             complement(48780..50045)
                     /gene="secD"
                     /locus_tag="PKU88_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPX68204.1"
                     /translation="MKSKSLILFLAIVLLISFFAYIAFFGLNIGKYEIVPAKDLIKLG
                     LDLRGGVTVLLEAKDKPDDPVNDEKMARAVATIRERIDTLGVTEPIITRVGADRIEVQ
                     LPEIQDPQRALDLIGQTAQLEFIEEETGTVILTGNDVKKAQAEFAPSKDGLGQTPVVA
                     FELSPEGAKKFKEATERNINKIIGIYLDKNPISLPKVESVIPDGKGIITGSATMEEAG
                     DLATLIRAGALPVPLETLSVTAVGPQLGANSFEQSVNAGQIGILLVFIFMIFYYRIPG
                     LVADITLVLYLTLTLFALAAMKATLTLPGIAGLVLSVGMAVDANVVIYERIKDELNLG
                     KTLRAAIESGFRRAFLTIFDSNLTTIIACVILFYFGSGPIRGFAVTLIVGVAVSMFTA
                     LTITKIMLKLLTSGGILKSMRYFGERGVK"
     gene            complement(50210..50725)
                     /locus_tag="PKU88_02595"
     CDS             complement(50210..50725)
                     /locus_tag="PKU88_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPX68205.1"
                     /translation="MIISYKHYKPNIGKNTFITENSTIIGRCIIGENCSIWYNTVVRA
                     DVNEIIIGRGTNIQDGCVVHCANDYKTIIGENVTVGHNAIIHGCTVGNNCLIGMGAII
                     IDGAVIGDNVIVGANSLITSGKKIPPGVLVMGSPAKVVRELAPAEIEGISHSAEEYII
                     LSKEYSKLSIE"
     gene            complement(50828..51013)
                     /gene="scfA"
                     /locus_tag="PKU88_02600"
     CDS             complement(50828..51013)
                     /gene="scfA"
                     /locus_tag="PKU88_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HPX68206.1"
                     /translation="MKHVKTINKRSLYDSVQKGGCGECQNSCQSACKTSCTVANQKCE
                     NIQNSRVRTGTSHKNTI"
     gene            complement(51111..51524)
                     /locus_tag="PKU88_02605"
     CDS             complement(51111..51524)
                     /locus_tag="PKU88_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HPX68207.1"
                     /translation="MRKFKSYTKMPPAEEKGINIYGVFLKSLGICFGVSFILIMLYAL
                     VLSFTSMSDSSMSMTIQIIMIVSITISSIYGGKKILKKGWFFGIVLGLVFTLLLVPLG
                     MCFGQSLSLDKYFAAKMLMGSSVGLIGGIIGINLN"
     gene            complement(51648..51923)
                     /gene="yajC"
                     /locus_tag="PKU88_02610"
     CDS             complement(51648..51923)
                     /gene="yajC"
                     /locus_tag="PKU88_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPX68208.1"
                     /translation="MQQFGWILPYAIVIAAFYFFLIRPQKKKEKATQEMRNSVKEGME
                     IVTIGGFYGKVVNAKEDVLTIEVGADKVKLKIARWAVGKVIENNEEK"
     gene            complement(52088..53206)
                     /gene="tgt"
                     /locus_tag="PKU88_02615"
     CDS             complement(52088..53206)
                     /gene="tgt"
                     /locus_tag="PKU88_02615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPX68209.1"
                     /translation="MTIKYELIKEEKRTGARLGKLHTPHGVIDTPVFMPVGTQATVKA
                     MSPLELKETGAQIILSNTYHLYIRPGHRLVEKAGGLHGFMNWDRAILTDSGGFQVFSL
                     NELRKISEEGVEFKSHLDGSRHFFTPEYVMEIENSLGADIIMAFDECAPYPCEHDYAK
                     KSMERTARWLKRCKEAHNNTEKQSLFGIVQGSVYKDLRIESAKRTVEMDLPGYAIGGL
                     SVGEPKELMNEVLEYTVPELPSDKPRYLMGVGSPDCLIEGSIRGIDMFDCVLPTRIAR
                     NGTVMTSRGKLVVRNAEYSEDFRPLDEECDCYACRNFSRAYIRHLIKAGEILGGRLTT
                     VHNLRFLQNLMSEIRKAIMEDKLTELRNDFFEKYGYEK"
     gene            complement(53244..53876)
                     /locus_tag="PKU88_02620"
     CDS             complement(53244..53876)
                     /locus_tag="PKU88_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HPX68210.1"
                     /translation="MKMTRIKHSIINASLYLENILAIVIILAIVIGMIDLAKYIVLIF
                     QTSTLQTYEVFQKFLGHTLLLVVGVEMVAMLVRHTPGSVIEVLLYAVARKMLISNAHM
                     LEFFLGIVSIASIFAIKKYLFISNLRDSEAINIFPASASISDINNTIGVKIPEDLADT
                     VGGLISRLSKQSCRDIHEGSYYRIADAELKVLSYNDGVIHRVWAAKYNES"
     gene            complement(53894..54919)
                     /gene="queA"
                     /locus_tag="PKU88_02625"
     CDS             complement(53894..54919)
                     /gene="queA"
                     /locus_tag="PKU88_02625"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPX68211.1"
                     /translation="MELKDFHYDLPEELIAQEPIPERDMSRLLVLDKNTGEIVHRSFR
                     DILEYLNEGDCLVMNNTRVIPARLFGVKRGSTGNMEFLLLNRKDKYVWEVMVRPGKRA
                     KIGAEFIFGDGELSGEIIEILEGGNRLVKFNYEGIFEEVLDRLGKVPLPPYIKKELTD
                     RERYQTVYSKIEGSAAAPTAGLHFTNELMHQLAEAGVNIAYLTLHVGIGTFRPVKVSN
                     IEEHKMHSEYYELTAESAEIINNTRKNGGRIICTGTTSARTLESIMQRKGSLKEDKGW
                     TDIFIYPGYQFKITDGLITNFHLPESTLLMLVSALAGRDNIMRAYKEAVKEHYRFFSF
                     GDAMLII"
     gene            complement(54934..56577)
                     /locus_tag="PKU88_02630"
     CDS             complement(54934..56577)
                     /locus_tag="PKU88_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPX68212.1"
                     /translation="MKSKKFAVLYLVITSVMFMILHADIAVPETIRIGLSFGQSQANI
                     FTLASETGMNILILEKGSYKNLLEVKNPKVVKVRRDEYYNIAGGKECEINYVRAAKYE
                     GEVVGPYHIQIGGVYANAEAALQVLKQVSSITQSVFLAYEEGWRVWSQLYLDESECLE
                     QIKIMKKEASDLKYSVVYPDKKRIQIIDNTTGKLMLLLNSEGNVKVTPKEVNGKVSSL
                     QYKGKKYRGSIVMQSFEESDITVINELSLDQYLYSVVPSEMPASWHIEALKAQAVAAR
                     NFALVTMGRHNAHGFDLCSTEHCQAYNGLAQENSNSTEAVNATKGKVITYNGVLISAF
                     YHSSSGGHTEDSENVWGAKTDYIRGVEDKFSLGSPHDNWTVELNRAELKEKLAQSGVD
                     LGEIRDVRILECSKFGRVTKLEVKGSKDTTVFEKEKIRSILGTRLLKSIWYDLKTDAD
                     VFVRGSILNSAEKGRTSNMYVVSASGKAVVKGKGNKVSVKGMNDTKAYNVIPDKYTFD
                     GKGFGHGLGMSQYGAKGMAEAGYNYQKILEHYYQNANVQ"
     gene            complement(56612..56827)
                     /locus_tag="PKU88_02635"
     CDS             complement(56612..56827)
                     /locus_tag="PKU88_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HPX68213.1"
                     /translation="MNFQYFAKIIIVLGIGMVIFGTLLYFGGKLGFGRLPGDIYIKKG
                     NFTFYFPIVTCILASIIMTIVFGFIRR"
     gene            complement(56824..57369)
                     /locus_tag="PKU88_02640"
     CDS             complement(56824..57369)
                     /locus_tag="PKU88_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPX68214.1"
                     /translation="MKLLLHMCCGPCAVYPHQVLSREHEITGLFYNPNIHPYTEYKKR
                     LDTARDFADKSGYKLITIDEYNLDEFLRNAAFREGQRCIMCYAERLERAASVAKSGCF
                     DAFTTTLLVSPFQKHDLIRRIGEEAGKKYGVEFLYKDFRGGFKEGVEKSRELGLYRQP
                     YCGCIYSERDRYMPGRKVGRK"
     gene            complement(57385..58380)
                     /gene="ruvB"
                     /locus_tag="PKU88_02645"
     CDS             complement(57385..58380)
                     /gene="ruvB"
                     /locus_tag="PKU88_02645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPX68215.1"
                     /translation="MEDRLITPKIIEDDIEIDTSLRPKSLEEYIGQSVAKEKISIFIQ
                     AANKRKETLDHVLLYGPPGLGKTTLANIISNEMGVSIRITSGPAIEKPGDLAAILTNL
                     DNNDVLFIDEIHRLNRSVEEILYPAMEDYALDIIIGKGPSARSIRLDLPRFTMIGATT
                     RAGMVTAPLRDRFGVICRLEYYTDEELFKIIKRSARILKIDTDEAGAFEIARRSRGTP
                     RVANRLLKRVRDFAQVKASGVIDKETADRALKLLEIDDMGLDDIDKKMMLTIINKFDG
                     GPVGLDTLAASIGEDSGTIEDVYEPYLLQIGFINRTPRGRVATNSAYRHFGIKKD"
     gene            complement(58410..58997)
                     /gene="ruvA"
                     /locus_tag="PKU88_02650"
     CDS             complement(58410..58997)
                     /gene="ruvA"
                     /locus_tag="PKU88_02650"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPX68216.1"
                     /translation="MYEYLSGRVDYIGEDYAVIDISGLGYRVFTSQNSMKSLKLGEIS
                     KIFTHLIVKEDDLILYGFATREELGMFKLLISVSGVGPKAAASLLNQYKASELAAAIL
                     SRDVAKLSKAQGIGKKISERIILELKDKIDTESAVGAIGAFGGSDEISQVIEALTALG
                     YNYSIAANAVMKLKDTNKPIDILIKEALRQLGTMK"
     gene            complement(59010..59501)
                     /gene="ruvC"
                     /locus_tag="PKU88_02655"
     CDS             complement(59010..59501)
                     /gene="ruvC"
                     /locus_tag="PKU88_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPX68217.1"
                     /translation="MLILGVDPGIAILGYGLVKYEANRFTVIDYGAITTDSGINMSAR
                     LTIIYDRLVDIIERHNPDAFAIEELFFNKNIKTALTVGHARGVAVLAGSKSGVQVYEY
                     TPLQVKQAVVGYGRADKNQMQQMVKVILNLGEIPKPDDVADALAVAICHGNSSKFSEQ
                     FRV"
     gene            complement(59953..60612)
                     /locus_tag="PKU88_02660"
     CDS             complement(59953..60612)
                     /locus_tag="PKU88_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68218.1"
                     /translation="MFEDRSIRRKRRYLLAAAFLLLISAAFAVGYFFNADIPEALDNP
                     DNQPSANLQIPDSLINPTTVRKPYEEVNAESNTEETANILEIDFPTPNTQLIFKTYYS
                     SCRHTVEKAVQAGSDEINMNEQQLKEKYSHWHLSGYSPPVVEFSRNIDTYCPGHYIIG
                     VDGGYIAIYVYDENGHKSLQEKTDISAAPLTPEDQQALAGGIVVDTEEQKEQTLEGFS
                     N"
     gene            60817..61701
                     /gene="uvsE"
                     /locus_tag="PKU88_02665"
     CDS             60817..61701
                     /gene="uvsE"
                     /locus_tag="PKU88_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HPX68219.1"
                     /translation="MTGLGFACISKSLKENGRFRTMSVKTYNSLKPDERIRRLRSISV
                     DNLYNTCRIILWCIENSISLYRLSSGLIPLAGCVPEWKWWEDRDIRNMCEKIKKIVDE
                     GKIRISMHPDQFCVLNSDKAEVVKGSIAILEHHNRLSNMVGNRILVIHAGSGTGGKSK
                     AIERFIKNFNMLDEDIKKKIAIENDDKVFNAEDILHLCEHLGVPMVLDIHHHNCNHEK
                     DNLPQLMDRIKSTWKGKRPKVHLSSGKTCPTDRHHADYIDFGDYRQAAELVKDEFDIM
                     MECKEKDLAVLKILTYCI"
     gene            61796..63727
                     /locus_tag="PKU88_02670"
     CDS             61796..63727
                     /locus_tag="PKU88_02670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPX68220.1"
                     /translation="MFINGKIPHLLQQTVLMTAFTLLKFTALYHFAGKPQDMLPAALR
                     TYTLLFAIYLLTNLVIGRKSFLIRIAFHEFLALIVLADTLYYKQFNILPEVADLQFLK
                     VLPTIWDSVGFLFSGIHLLLFADALILMTHQYALNRQEKASAPGILPSVIAAALLLAI
                     IFTDFSFSDIKSSGQAYEKFGLLHYHISQIPGVLQSNSNSSSRSIGKAQIPAEVEEAA
                     KEPRCFGIAKGRNVIVIQVESLQDFVINMHYEGQELTPNLNKLLLKDTIYFNRFYQQL
                     GKGNTSDAEFVTQNSLYPSIDIPAYRKYENNRFMGLPMIMRETGYGTYAFHAYKPDFW
                     NRQNMYPMLGFDRFINMNDFNLNETFGMGLSDKEFFEQSAAYISTANQPFYSFLVTLS
                     SHHPFGLPQKYKKIQLQPAHKDTLFGRYIQVINYTDEAIGLFLEKLKEYGLYDNSIIA
                     LYGDHRSIPNGVPENDKLMSELLDHEYTFDESLNVPLIIHIPGTNIIETNTIVGGQMD
                     FLPTMLNLLGIKEIRPKLFGQDLLNAKNGFVASQTGMIKGSFIDNEKIFVMSRDGVFE
                     NSTAWKLDTREPVDPEFCRNGYERALREIKKSEYILANDLIHEFITLGKSHTTFSSAI
                     EAASGYIERIAALFKSIVE"
     gene            63885..64577
                     /locus_tag="PKU88_02675"
     CDS             63885..64577
                     /locus_tag="PKU88_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68221.1"
                     /translation="MKKSIVLLSILALLAAVFAAVAAKPLKIGDVIGKIYYTDIKTYI
                     DGKLIESVNIGGRTMISAEDLQYMGYLVEWNDTARTLNITSNTSHNISGKVIIPDGKV
                     APKGGLNVEIAAFINPNPDPSVISRGYGPEFGKEIRLVIPEGHSSIEYIISVPYKFEP
                     RYITAILPGGESGEPEKLPDIKPYYVLRFRYSYYLGNHKVEHGDFMEGYLDMSTGDKT
                     GVDYTFDNIDLK"
     gene            complement(64647..65513)
                     /locus_tag="PKU88_02680"
     CDS             complement(64647..65513)
                     /locus_tag="PKU88_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPX68222.1"
                     /translation="MKKRSLGLILFTILIIISIISAGCSGKAANESAPPEPSTASSAA
                     EEQKTAGEMPATAPSQTPDRKLIVMLSMDMRVDNIEKSMLDAEAMAAASGGYTRESYQ
                     SESSGRLTIMIPAGEADTFVSSLRSLGKTINSNKKTEDVTDSYFDTQVRIKNLEAEIE
                     IMRSLLQKPGWKVSEILEIEREIRRLTDELESLKGYITNLDRQITYSEVRIRFEKSQI
                     SIDSASQDSLGYKLQLALKRGIDLLINIVTSLLSLIAFILPISPFIAAVYFLFRKPLR
                     RFKRKDKQDPGQ"
     gene            complement(65753..66499)
                     /locus_tag="PKU88_02685"
     CDS             complement(65753..66499)
                     /locus_tag="PKU88_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPX68223.1"
                     /translation="MSGHSKWANIKHKKGKADAMRGKIFTKLGKEIAMAAKNGADPST
                     NGKLADVIAKARSNNMPNDTIQRAIKKGSGEMEGVDYEEIVYEGYAPGGVAVIVTALT
                     ENRNRTAGDVRHIFDKNGGSMGVSGAVSWMFDKKGIIIVENDGTTDEEELMMHALEAG
                     AEDFNAEEDSFEIVTSPESFSEVRQKLEEAGIPMASAEVTMVPQNYIEVTDPEAVKKI
                     NKMLDMFDYNDDVQDVYHNAELPDEEDDLI"
     gene            complement(66578..67315)
                     /gene="nadE"
                     /locus_tag="PKU88_02690"
     CDS             complement(66578..67315)
                     /gene="nadE"
                     /locus_tag="PKU88_02690"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPX68224.1"
                     /translation="MINADTVINEAVEWIKNTVEGAGAKGVVFGLSGGIDSAVVAALC
                     KKAYPSDSLGVIMPCYSNPIDEEHAIAVAEAIGLETKTVVLDKAFDSMKAVLGVRESD
                     PRLAVANIKSRLRMITLYYYAGLRNYLVSGTGNRCEFTIGYFTKYGDGGADIMPLTSF
                     VKSEVVELAYKLGIPEAVIIKPPTAGLWENQTDESEMGMTYTELDNYILTGAAEEHIK
                     KKVDAMNKRSEHKRTMIPRFVPSEKQD"
     gene            67557..67916
                     /locus_tag="PKU88_02695"
     CDS             67557..67916
                     /locus_tag="PKU88_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPX68225.1"
                     /translation="MKNKNHLKTALIGFAAGLVNGIFGSGGGTILVPCLVFLMGIEDH
                     KAHATAISIILPISLISSFIYFRYDVTDLSLTLKVAAGSVLGALFGSFVLNWVPINTL
                     RKIFGMFMILAAIRMVF"
     gene            67916..68302
                     /locus_tag="PKU88_02700"
     CDS             67916..68302
                     /locus_tag="PKU88_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPX68226.1"
                     /translation="MLTVLIGFASGIISGMGIGGGTLLIPALVFIVGTKQQIAQSINL
                     LVFIPSASAALFVHARKHNIEKGLLFKLAVTGCAGAVIGSLAAVNLDSGILKKIFGTF
                     LFIMGIYEISSKSREKNKKTGKEGCR"
     gene            68299..68454
                     /locus_tag="PKU88_02705"
     CDS             68299..68454
                     /locus_tag="PKU88_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68227.1"
                     /translation="MKIRKLLAAYALARGVMKYRRSRQNSPADKFEKCENYYRCDDDE
                     KDGRGNY"
     gene            complement(68456..69346)
                     /locus_tag="PKU88_02710"
     CDS             complement(68456..69346)
                     /locus_tag="PKU88_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX68228.1"
                     /translation="MNRNKVGYVYVLLAAFFFALIAVIGKTVINSGIKIFDLLVLQYT
                     ASFVFMLIYFVIAGIRKLSLPKESLKAVLTQGLIGSSCTTVLFYLALEKLNAGIASML
                     LFTHPVLVSIYFMATKTRKITRRSNLALLTAFFGSIMVINVFNIGIAGTPFIGFVFGI
                     MSSCTYAFYNIYADVKLKDFEPLVIAFYTNLTTLAVSLILHPGFFRFEFLMTSELLIY
                     IFELAVVSGILPVIFLYKGIKLIGADKASIVATSELPITILLSFFVLGERMGLVQLAG
                     ILLIMGSIIILQYEGKLEGG"
     gene            complement(69363..71129)
                     /gene="hflX"
                     /locus_tag="PKU88_02715"
     CDS             complement(69363..71129)
                     /gene="hflX"
                     /locus_tag="PKU88_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPX68229.1"
                     /translation="MENTIGIKKSLLKRLENLKESTVEKYQLVSEEIIAEICDITEET
                     GKEIAVYIDRKGTVLDVSIGDENTVTLKDFNFRRSEESLSGIRCLHTHPNGHGGLSEV
                     DISALKRMKFDIMAAVGVSNGEMTNATMAFLGVDSDKQFQAVVMGPYSYNRLMKVNIF
                     DIIVEAEAGIKNIRKASKEVDIDAERAVLVGLEDEEALEELGELLKTAGGCEVGRMVQ
                     SRDKKDTVFFIGKGKLKELMLLCQAVEANLAVFDEELSGAQIRNLEEALGLKVIDRTQ
                     LILDIFAQRARSREGKLQVELAQLKYMMPRLIGLGQQMSRTGGGIGTRGPGEKKLEVD
                     RRRIRDRLNDLENELKQLKKHRDLQREGRVSGKAFQVCLVGYTNAGKSTLLNSLADAD
                     IYAEDQLFATLDPTTRRVTLGNGKEVLVTDTVGFIRKLPHDLIEAFKSTLEEEVYADL
                     LIHVVDGSNSDYEDQARVVIDVLSELGAGDKASIMAINKIDKCPGRFSPEEYEDMENT
                     VLISASERTGLPKLLALIEGFAAKQSRTVELMIPYAEGSVVSEIYKSADEVTEEQYGE
                     DGIRIKAVVDEISFGRLKKYVI"
     gene            complement(71212..72303)
                     /gene="rsgA"
                     /locus_tag="PKU88_02720"
     CDS             complement(71212..72303)
                     /gene="rsgA"
                     /locus_tag="PKU88_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPX68230.1"
                     /translation="MNNLISYGARERYFNEAALYPDLKLARVVSQYKNLYKLVTETGD
                     FLAEISGKFRYEAKGLSEYPAVGDFVMVDLSLGSSSNAIIHRVLTRISAFQRAAAGTE
                     HQTQVVAANIDTVFICMSLNNDYNLNRLERYISMAWNSCATPVVVLTKADLCEDLSVP
                     LSEISAVAPGIDVVVTSSNDRASCDKLLPWLQPGMTASFIGSSGVGKSTLINRLAGEE
                     LLMTSGIRKDDRGRHTTTRRELMILQQGGIVIDTPGMRELGLESVDLSKSFSDIDDLA
                     MKCRFRDCTHTTEPDCAVRKAIEDGTLTERRLQSYHKLKRETCYDGLSFKQIETQKLN
                     AMFGSVGGMKNAKKFIRQNNKREGNTKKL"
     gene            complement(72555..73298)
                     /locus_tag="PKU88_02725"
     CDS             complement(72555..73298)
                     /locus_tag="PKU88_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68231.1"
                     /translation="MKAFKTLFKTEFKLSLRGMDMFIFAICMPVVAVIILGSIFGNKP
                     AFDGASYSFLEQSFGAVATIAICAGGVMGLPLVISDYRSKKILKRFKVTPISPTVILA
                     VQVVIYAVYSFASLILVYATAAIFFNYHLNGSWLQFLGAYLIVMVSMFSIGVLVGGIA
                     PNMKMAGIIASLLYFPMLIFSGATLPYEIMPVTLQKAADIMPLTQGIKILKATSLGLP
                     IDSVLAPVIVMIVLAVICTGISIRFFQWE"
     gene            complement(73285..74019)
                     /locus_tag="PKU88_02730"
     CDS             complement(73285..74019)
                     /locus_tag="PKU88_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68232.1"
                     /translation="MESVIKVEKLSKSYSNVTAVKNLDIAVRRGEFFGLLGANGAGKS
                     TTIECILGTVKQDSGVVSILGMNPQKDRKILFERVGVQFQEANYQDKIRVMELCEITA
                     SLYKTPLDYENLLKQFGISEKLKSPVSELSGGQKQRLFIVLALIPNPEVVFLDELTTG
                     LDTRARRDVWNSLSSLKEKGLTILLTSHFMDEVEALCDKIMILKNGKSIFYGTIQEAV
                     ASTPHEKFEDAYLWYTEERGEENEGI"
     gene            complement(74079..74840)
                     /locus_tag="PKU88_02735"
     CDS             complement(74079..74840)
                     /locus_tag="PKU88_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPX68233.1"
                     /translation="MNTYKTAEIAKFIGIHPNTVRLYEELGLIPKPERKPNGYRIFTD
                     FHIEQFRFARTALKVEVLQNGLRKQAINIIKTSAAKDFDRAIYLTNCYLKQIKSELQK
                     AEETIAVTEMLLSGEDKASEDMLFTRKEVLNHLLITMDTLRNWEMNGLLTARRKQNGY
                     RVYTSEDIKRLQIIRTLRCANYSLAAILRMMNALSNNPETDIREIIDVPPKDDDIISV
                     CDKLLTSLRYAEKNAFVMLTHLDKMKKLFISNPTL"
BASE COUNT        19985 a        18443 c        12987 g        23599 t
ORIGIN      
        1 cagctcctta ttaattccta cctattaagt cggatttcaa tttaaggtta ccatagcaga
       61 aaaacattgt caatattaat ttctactttt gtagtcggaa ttaattctta ataatgaatg
      121 tataccagta ggagtcacag ttaagaaccg cgaactatct tttagtcctt gtcctgtctt
      181 ttgtgcttaa gtattgaggc cgctttggat ccattgttaa gctgtatatt aaggatcttt
      241 tgaagtcata aaaattgaat ggtgcccaag gtgctaaata aggcactcca aaactgttta
      301 gtgaaacaag ctgggctaaa ataaaggtta aaaacaaagt aaacccataa aaccccaaca
      361 taccggtaaa tagtagaagt ataaatttca atattctgaa tggatttatt aatgtgtaat
      421 caggaatggc aaaagaggca agcagtgacg tcgatgcaat gataagcaaa agcggactga
      481 aaatttctgc cgcaattgca gcttgcccta ttactatcgc ccccacaata cccacagcag
      541 ggcctatctg ctttggaact ctaagaagtg cttctctaag caactcactg ataaactcaa
      601 ttgtcaatgc tcccattagg gcattgaatg gtacatttac acgcatttct gcgagagata
      661 tcgcataatc gcttggtaga atatcagtat gaaaggatgt tattgcgaca tatatgcttg
      721 aaacggtaaa ggcaaaaatt atagcggcat accgtatcag cctcataaaa agcccgaaaa
      781 acttgttgtc atacctgtcg tcgcatgaat aaaagaattc gctgaaggtt ttgggagcaa
      841 gcaaagctat tccgctgcca tctaccaaca ctaagacctt tccttcaata agggaatgaa
      901 aagccatatc cgaccgttct atcaagccta tattagggaa caactgcatt ttactattta
      961 acaggaagga ctgaagttca cctgattcgc cgataccgtc aacgtctatg ctttctatcc
     1021 ttttttggat ctcggtaaca acagttttgt tcgcaatatc ctctatataa atgacagcaa
     1081 ctattgtctt tgacctgaca cctacctcgg actttttaat ctgaatattt ttatccttca
     1141 gcctatacct aataagtgaa agattcacat caatgttttc ggtaaaacaa tcttgagggc
     1201 cgcgaatggt atatgtaagt tgcggagtag gaatcatcct atgttctatt ttttttatgt
     1261 ttataacgat gtattcttca tctgtggaaa aaagagctac cgccatccct gagaggatat
     1321 attcttctat tttgcttatg tctgattcta acctgcaatc atcggcaaaa attatggtgt
     1381 ctattgtcat ctgtgcgttt atgggctcct gagccgagga acaatgtaag ataagtggtt
     1441 tcacaacgct ttccacaagt gcagccctat ccattagctg catgatatag aacagtttta
     1501 ccattccgtg gtggaatggg atcgaccttt ccgtaatatc cagattcttt gatttaatcg
     1561 aatctgctat cttttcaaaa tcggaccgtt ttagcatgta tttcctcctt tcattttctt
     1621 acatccgttt caaaatccat catccacgat ccgctcagta cgacctttac atcaatattg
     1681 acacttgcct ccggaaattc attctgccaa tccatgcttt caaactcttc agggaatttt
     1741 acacgaaact cgtcatcaaa ttggagataa tcgcacttaa aatgtttttg cgcctccaaa
     1801 accgcatcct ttatttcatt tttaagaatc tccgtgagga ttttggtaac cccttcatta
     1861 gaaatgtcat caaaattgta aggtgttttt ttatctccgt acagaagctt tgcttcaaag
     1921 tcaaatttca aatcaaaact tacattaccg tcctcatatg atggttcgat ctttctcttc
     1981 ttcaggctcg tttctattgt gaattcattg cctttataag gcactgtata aaaaaatctg
     2041 ggtttatcgg tctttagaaa taccaaggct ttcgaatctt ccacaggtat aaagccgttt
     2101 attgtggtac cattgactac cgaatatcca actaacgaat tcttattatt ccgcaagcct
     2161 atgcaaggaa tcaagaatcc cgtgtagctg cttgagaggt tttccagcat tcttgaagtt
     2221 gttcttgtga aagactgtcc ttcttcatca agggtttcca gcatttcttc aatagagaaa
     2281 cctacagata tatcattttc attgttaatt ttgaataatt tttcgggatc cacagtagta
     2341 atgactatca tagtcttttt tctatatagt tcatcagttc taaggcggta tagatactca
     2401 accaaatgtt cttttgcaaa attttccgtt agtataagcg ttcttatcgc accgtaatac
     2461 gcccttcttt ccaattgcat atacaatttt ctcctggctt cgtgtatggt tttaccatgg
     2521 ctgtaataaa atatgtattt tctgtctgca ctgctgcttt taccctcatc cttattgctt
     2581 cctgtaatat ttgctatctc tgcgtaatag tatatctcat cgtctttttt atcaagacag
     2641 atactcatca atatactctg ttcgtgtata tccttagaat cagcacaagc tgctgcaaaa
     2701 gcaggaatac aaagtgtaaa aaccagaatt aagaatattt ttccggattt tttcaatata
     2761 cttcctccta tgatatatta gtcctttttt tcacttttgc aagaataaat gctgccgcag
     2821 gtataagaaa actcgagatt attacaaggt agaaagaaaa aaactctaag agcttttcta
     2881 tatcttttat attaagagcc agcaaacaca aaatatataa aattactcct atagcgacta
     2941 caacaatatg acgttttact cttgaaaata ttctgcaggc aaactccaga gctgcagtaa
     3001 aaactattat cattccgcag aataagcttg ataagccaaa agtaaggtat aatatatcca
     3061 atctttcaat aacaggagct tcagttattt ttatcgcttc aataaatgca tcatttaata
     3121 gaatcgtgtt gtttattccc aaaatcatta tggtgctttc tactataagc acataaaaca
     3181 gcccaataaa caagagtgta agaaatgcga tcttaggagc ttccttattt ttttcagtaa
     3241 atggcacgat taagagaacc tctatccctc caaatggaac aattaattcc ttcattgctt
     3301 ctgtatatcc ttcaatatca gaaggagcaa aaaaaggtaa tacattgaat ttcgtgcctt
     3361 ccgtcagcat aaaaacgcat gtcaccactg ttactaacac aaataaaatt ccatatatct
     3421 caaacatcct tgcaatattc gtaacgccct tgttcgccgc atatgcaaac agcaggattc
     3481 caaacaacaa tatttcatat ctgggtgttc tgggcagaaa atttgatgtc atcaaattaa
     3541 caaatgtcag ttttagaaat acaccgacaa atataaaata taacatgaag taagaagcta
     3601 ttaagtatga aaaaaacctg cctgctattt cctggctata gtcaaaaaat acttttcccg
     3661 gaaacgtgtt gtttaatttt gtgattatta ctgagacaac accgaaaata acagaagagc
     3721 acataattgg aaccatgctg ctcctgcctg ctgtttctgc cattgctttc ggaagaaaaa
     3781 ttgtggtata tgtagttaaa gtcataacta ataagaaaaa tatctgcctg ttcgttatac
     3841 tgttagccat agtcttactc caatttatag aagttctaaa tattttatcc ccttcctccg
     3901 cttttatgct caaatgacta tactaaaagg tgcggcttgc gccgcacctc tttatataaa
     3961 ttgcctcccg atatattgct atatcgaaaa gcaacagctg tactataata catactatgc
     4021 aaggctttta aaaatgttaa ctgtgaagct gttatgatat gccgacaccc atatatttta
     4081 tgcctgaccc attaaactta tacttgtcaa gggctctgca gccatcgaac aacagtgccg
     4141 ttttcaagga tgccgaactg ccttcgttca aaaattccag atactcctgc catggtgtca
     4201 taaggactgc cgcatcaaag cttatggctg tttttttata tccctccagg aaacatatcc
     4261 cccggtatcc ttcaagtgct tttcttgctt tttccgttgc catgggatct gctgcatata
     4321 ttaccgcctc tttttcagaa agaagcttca caagccttat agccggagat tctcttatat
     4381 cgtcgctgtt ctgcttgaaa gcaaggccaa gcaccaatat ctttttgcct tccaggccgt
     4441 tcaattcttt ttccagccta tccgtaattt ttccaacctg catctcattt atctccgcag
     4501 tagctctgag cagctttgga gcatagccga ggctttccgt aaaggctatc aaagctctta
     4561 aatccttagg gaaacagctc ccgccataac cgcgtccgga ttttaaatac ttgactatct
     4621 caggccttat aaattttccg tcaatttggg gactgattct tttgtcaagg gtcactccat
     4681 acagtacttc ttccgcatcc aatccgccga ttgcttctga tatcgccgca atttcattgg
     4741 aatatgaaat cagcattgca agcagtgaat tggaggcgta cttaataagc tcggcggttc
     4801 tcaggctgac cctgagaagc ggcgcctcga aataaccttc ataaaccatt ttgaagtgct
     4861 catagctcct gtcatcatat gcccctatca caattcggtc agggttcata aaatccgtta
     4921 cagcttcacc ttcccgcagg aactcagggt tcatgacaag cccgaattct ccagccttct
     4981 tccccgattc ctcttccagg atattcttta caagggtatc tgtggcagat ggtattaccg
     5041 tactttttat gcatataaca tgataggggt tttttatcct cagtattctg cctatgtctc
     5101 ttacagcctt ttctacgtta ttgaggtcta tactcccgtt cttgtatggg gtatctacac
     5161 aaacaatgga aatttcactg cttgccaccc catactcaat gtcagtggtg gccagcagcc
     5221 ttccttcagc aaccgcttca ccaatatatt cagaaagata gggctcgaat ataactgcct
     5281 ctgagttgtt aattctatct gcaatttctc tttgactgtc tacacatatt acatcatggc
     5341 ctttcttcgc caggcatgct ccgcatacaa gccctacata accggtacct ataattgata
     5401 ttttcatacc ccttctccta ttttcccata taaaaataat ccgtcacttt cctgtcaata
     5461 ccttctatct tctctatggc atctcttaat gccttgctgt atatcccgca aacaacgcat
     5521 gggatctcgg cgccgcataa tgcgacagta tctgcgtacc tcttcagcct cagcccgctg
     5581 aaatgataaa agatcagcct gcttccgttc acaagaatat tgccgtccat ttcggaaaaa
     5641 tcatatttga aataattcca ggaagccgca tttgctccgg gaatatcaat atactcaact
     5701 ccattaaaca ttccgctcat ggaatccaaa tatccctgat cggcaaatct cccattatca
     5761 tatttgcaat agcaccattc aatacatctg tccttccacc atttcaatgc cttatacgag
     5821 tcattgcttc tcttgaaggc tgcaaagcct ccgttagtct ttctgttcac cttatgggta
     5881 gtcagaagaa catgccacct gtcttttctc ctgaaaagct tcattgggtt atcaaagaaa
     5941 aaaatatccg aatccgcata aacaaatatc ttgacatctg cgaacctttc atacaaataa
     6001 agcagcagca ccggcttcaa agtccagcag tattcgcttg ggcttctctg acccttcaat
     6061 tccctcagtt catcattttc ctgttcttca gcagatataa ccagcatacc gggaagcttt
     6121 aaagcatcaa ggaccctccc tgactcccgg tcaactgcaa gaactgctaa aaatgcttca
     6181 ggtatgcggc cctttaagga attatacatg gctatgcatt gatggagcct gtgttttgtt
     6241 acgattgtac agaacattat attcaattca accacctaat ttattgctta tggcttccca
     6301 gttttcaatc atatcctgca ggtctttgga agtgtccgcc gcctgccaga agcctctatg
     6361 ctcgaaaact gccagccggg attccgctgc aagcctgggc agaacgtcct tctcaaatga
     6421 acagccttca tggtcaagat aatcaaagat tgctttcccc agaacaaaaa aacctccgtt
     6481 tatcagccct tctatttctg ttttttctcc aaaggatgct gcgagtccgt cccgggcctt
     6541 caaaattcca tattgactct ttttttctac agccgtcaat gtagctgtta ctcccatttc
     6601 ctgatgatat ttcagaagct tagcaatatc tacatcacaa agcccgtctc cgtaagtcat
     6661 aaggaaagta tcttcatcaa taaaaggctg tatccttttt atgcggtctc ccgtcatagc
     6721 atctgcacct gtatctataa aggttatctt ccacttttca ggttttttca acaggtacac
     6781 tttgttttca ttgccgctga aatccaaaat gaaatcattc tgcatccaat cataattcat
     6841 gaagtactcc ttgatctttt ctcccttata ccccaagggc aatataaaat cattaaagcc
     6901 gtatctgctg tatattttca taatatgcca cagtataggg cggcctccga taatggcaag
     6961 aggcttcgga atatcctccg ttacttccct catcctcatc ccatacccgc cgcaaagtat
     7021 taccgtcttc aataacatca ccccggcaat ccaaaaatta ttatatgata atcatcaaag
     7081 cagccttaac ggataaatgt ccgtatcccc gggctcatat tgtcttgtgg cggcagccag
     7141 gataacaagc tcctcttcct catcattcct gaaacagtgg gctgtattct cgggaacctt
     7201 tattacaata ttgtcctttg acgatgctct gaatacatcg gtgctgtcgg tttccaaatc
     7261 cctgagggct atggtcgctg tcccttttat cacagaaaaa aattcaacgt tcttcttatg
     7321 gtaatgattg cccctgacgg aatttccctt tgtgtaaagc acgtagattt cttcaacaga
     7381 ctcatcagca ccaagcatat ccattgtgaa aacctttttc agttctcccc tgccatcttt
     7441 atagggactg attgtaataa cctcatacct gcttttcata aaaacacttc cataatatct
     7501 atatattact ttcctgctta taccagtgat acaaccttcc aaggccggct tccagctcca
     7561 ccttggggta ataattgaga tagcttttgg ctttgctcaa atccgggcag cgtctttggg
     7621 gattatcagt caaataatcc ttttccttgc tttttttata ctcaatagcg acatttccga
     7681 ctatccctgc aatcagctct gcaagtccct tcatgctgat ttcggttctg tcattcccaa
     7741 tattgaaagc ctctccgtcg aactccgaca gcaatacaag catgaagcct ttgactgcat
     7801 cgcttatata gcagaaactc ctggtcgggg ttccgtcgct taatatctgt atctttcgct
     7861 tatggatggc gtcattgaaa aaatcgggta ttaccctttt gtcattcagc atcaagccgg
     7921 ggccatatac attaaaaggc cttgccgact taatcggcac cttgtattcc ttataatagt
     7981 taacaaccat tgtttctcca agccttttgg attcatcata gcatgctctt ggacccgtgc
     8041 aggagacatt cccgttgtaa tcctccccgg taggtatgtg ggactcatca ggatcaccat
     8101 atatttcact gcttgaaagg ttcagaaagc tcttgatgtt ttttttcatt cccagttcaa
     8161 taaggttctt cagccccagt acatttgttt caagagtctc aatgggatac ttcctgtaat
     8221 aagacggcga agctatgctt gccgcattta ttatataatc aatgtcgtaa tctatttcaa
     8281 tcggctttga tatatctgca tttaaaaaca taaagttttc gttatgcctg atatgtccta
     8341 tcctttcagg cacaccggtt acaaaattat caacgcaaat agcggttaca gattccataa
     8401 aaatattatc gttgcagaat ttcagcaaat ccatgaaata agaacctatg aacccgaccc
     8461 ctccgctgat aagaaagctg cttccctcta taaccttcaa cgtccctccg atttccagat
     8521 atatatccct tacatcctcc tctattattt tatttatact caattttcat ctccccttca
     8581 cagaatactt gtcatggttt ttcctgaacc attctaccgt cctgtcaatt ccttcctcaa
     8641 ggttcaccct cggctcccag cccaatctgg ttctgatctt tgtcaaatca ggctgagggc
     8701 tccataaatc attttttcta tactcaactg ccccatagca tactcttttc cccgtttcaa
     8761 accttttcat aattagattt acataatgct tcagcttgaa aatactgccg ctgccgatat
     8821 tgaatatttc attcctgact tccttattga ccgcggccat cataaagccg tcaattatgt
     8881 tttcaatgta gcagtaatcc ctgtactgct cacagggaga cagggagact tcaccatctt
     8941 ccagcgcgga taatatcacg tggggaaaaa acttgtgact gccctctttt tccccaaaaa
     9001 ccccgaaggg tatgatagtg actatgtcaa ggttataccc ggctgctgcc tggtgagcta
     9061 ttatagtggc agcccctttt gtactgccgt atatgtttgt cgggctgtaa tactcctctt
     9121 ccttaattgc gcctttctta ttcccatatt gcatgcttgt tcctgtattc agcaatttct
     9181 cgcagcctgc cagccctgct gccatgacaa tatttaaagt acccattata tttgtctctg
     9241 cagccgccac ataatccttc tgccttgaat ccaccccata tgcagccatg tgaaatacat
     9301 attcgggttt tattcttgcc atacacagtc ttactgcctc gttgtccctt atgtcaatgg
     9361 caatcatcct tatggaatcc tttacctcgt ccagcctcca aagatcagaa gcatccctgg
     9421 ctgccgcaaa aacttcacag ccatacttaa ccagcctgct gactgtatga gacccgataa
     9481 acccattgct tcccgtaacc aacacctttt tattccttaa caacccggct tccatactgc
     9541 caccaccata tcaatccata ttttctatga ttgctgaatt tgatgccctg taccaggcgt
     9601 tcataccgga atatactttt gatatctcat taatatatgg ctttattttc ttgattgact
     9661 ccgcaactgc ccttgcataa ggaaagtata tgagttcctt tgcggagctg cttaccttca
     9721 ggctgtccca cttccacagg tcgaattcat tctcacccag aatcatgaag ctgctgaaat
     9781 gataaaatac cagaaggtct cctccgataa atatatcctc gcccctgcaa tgcacttctt
     9841 tatcttccag gttccacact gctgcattca taccgatgtt ttccgccagg tatgcactcc
     9901 catacggttt taagaaattt tcgagatatt tctgatcagc gaatctgccc tccgaaacaa
     9961 catcaaagca ccattcaatg cacttttttc gccaccactt cagatatgac agcgctgtgc
    10021 cgcacctctt gaaacctatt attccggcat tgaaccggcc cttctttcgg tataaatgct
    10081 tgtaaggctg tgaaaaatta tgatgggtca gaagcaccgt atgcctttcc agggctttaa
    10141 agcacctgtc aggccttgaa aacaagtata tatccgcatc taaatatagg agtgaggcca
    10201 cattttcata tttctcaaat atatgcagca taaacggcgc tttaagtgtc cagcagtact
    10261 cataatcctt cctgttcttc ttaactgctc tcagcttgtt gtcttctatc tcagaaaccc
    10321 tgaccaatgt cacattttcg agccccatac ctgtaagtat gtcataagcc gcatcctcca
    10381 tacagcatat ccatatatga aagctgtcgg tgttgctttc tatggaatcc agcagtgcaa
    10441 ggcatttcgg caggtattca agagttgtca atgtgcagaa gcaatactga gaattgtgtt
    10501 tatcctctgc agctcctgtt tcagaaattt gttccgtttc ctctgcattc cctcgtttaa
    10561 gctgcagcag ggcagcctcg caggcagata tataaggcat atatatgctt cggacaaccg
    10621 cttcggggaa cccccaatct ttgtaggtac aaaggtcata atcgtcagtg cctgcatacc
    10681 tgaagccata gtaatgaaac agcaccagcc ttcttgcatc tatatgcctc ttctcttctg
    10741 cggtaaacct ccatagtatg aagggcgtca tattgattcc ggcattcttt atcacagcta
    10801 ctcctttaaa tctttccggc cagtcattca catacatctg atcgctccac agcccgttct
    10861 ccatcctgtc atagcaccac tcattcactt tagactgaaa ccatttcagg cattcaaagg
    10921 aattcgcatc tctcctgaag cccaacaagc ctgtattgta tatgccgtat atccggctca
    10981 ggtaagcatg ctttccggtg tacctttcct ctgtaagaag tattgagtgc ctgccccact
    11041 ccaaatatat cgggccgggg tcggacagga acattgtatc cccgtcaagc cacattatat
    11101 ggtcaatgta attatagttg ttaaaaatat atagaaacgc cgagggcttt acagtccatg
    11161 catattcctt gtcattcctg tcttttcttg cttttgtaag ctccgggtaa taagactcta
    11221 ttgcactgac aggaataagc acagtatttt caagcttgag ggcagcgaat gaagccaatg
    11281 cttcatctcc catgcatata ataaaaaaat taaattcctt gtcatgttta accattgaat
    11341 tatataatgc aagcacctta tacttataat cactgcttgc tattgagcaa tagtaatgga
    11401 tcatcgatta cacggcccct cctaatcaat tcaaaaacgt ctttatttca gaatatgctg
    11461 tttatcgatg acaagttact taaaaaatat atttaagggc cagcatatgc tagcccttta
    11521 tcctgttatt caaaatattg ataagtttat tcaattcaat gcctctcgat tcggcagcct
    11581 gccgaagggt cctctcctga gatccccctc agccgcagcc tgcagacagc agatcatagt
    11641 atctcaatac acctttagtt cttggatagt atcttataat ctcttttatt ttcatttcct
    11701 cgcttatcat attgcattac gagataggat atgggggaat cggtactcca agctgagggg
    11761 gattgaatga agggatatcc tccatctcaa attcttcaca gaagtcaaca tctatcggtg
    11821 ctacatcctc ttctatctgg caatacccca ggacaggaac acaaagctgt acttcagcgt
    11881 caaactttac tgcaataaac gctcccaggt tggtaaccag tatctcaccg gttgttgtat
    11941 tgttctcctc atcaataact gcctcaccca ggcattgaag ctcaaatctc ccttgatcaa
    12001 ggaagtgcag cagtctttca aaattttctt ctgttacatt gggatagctt cttctcacac
    12061 tgaatttaaa gcagagttct ccgcagggcc cttctgaaag gttagttccc tcaggcacat
    12121 caagtacttc aagaggaata atgtttcctt tggcatcctt tccaaacaca tcgcagcagc
    12181 attttgccga gaatttaagc ctcttatctg aatttgttga aacatcctta atttctacat
    12241 tactcagctt gcagttcttc agtatcaaag caggctttat aatatctcca tgtggatttc
    12301 cgcagacttt gaacttgatg ccttctacac agtcccttaa aacaaattcg tcaaatacct
    12361 tttttacctt aaggcactca aaaaacaaat ctttacattc agtcgacccg cctgttttta
    12421 cctccagcct ggtggtccct gcacataata cagttggtaa tgaagcattt ggcactgtca
    12481 ttttctcacc ttcctataca tttacatttt ttcattgctg tctaatttat aatatggaag
    12541 tcaggacatt tttgtgaaca agttaaagca aatttaataa attttttaga caaatgcaca
    12601 atatacgaat aatgcgacgg ggacatgggg acggggacat ggggacgttt cccctgtccc
    12661 acttccagtg ggacacgcga aacgtcccca tgtccccgtc cccatgtccc cgtcccccat
    12721 gtccccgtcg cattattctt cagatgcgca agtccagtca tataagcata aaaatatcca
    12781 tataaatata gataaggaaa tttgaggtgg tgattatatg atagatataa acccatcagc
    12841 aatacttgag gattcaaagg gcaacaggta cttctttttt tacagggatt cttcaatata
    12901 ttacagggag gttcccatta agggggatat aaaggataca atgcttataa gccaggcaaa
    12961 cgcagatttt gctgctgtca tagatgcgga tgattccata tacctgacct gcaacagccg
    13021 atataaggga gtcctgcttt ttatatatac aaacagcggt tggaaatttg aacctgtcgt
    13081 aaatttgcac aacagctcca atatatttat tatggacatt atagtgcaaa acggttccat
    13141 acatatattc ttttcaaaaa agcttcctat tgccaatatg tacaatgttt accacattaa
    13201 caagaatata aacgataaga ccccttatat agagtattcc tggaggaaga acagcctttc
    13261 ggaaatatac tctcaaaaca ttgaaagctc ttactctctg ctccctttaa gaaacggtat
    13321 tattcactat gccagtgtat ggtacgatgg aaccctatat tatatcaatt attactgcta
    13381 cgacgattcc accaaatcct ggcttcacaa aagcctcagc atatcataca aaaaccaggc
    13441 gtttataaag ctcatacatc aaaataagaa aataaacctg ctctgctatt caaatgaaaa
    13501 cgagggaagc aacatacatc atttccttag taaatcttca agcagcagcg aaatagactt
    13561 cagggaactt ggaaatgtcc gtatcgatac caacggaatg ataccgctgt tttattccga
    13621 tgataaagca atacagatgg cctggataaa ggatcatgtt ttccatcaat acacctttga
    13681 cgattcagcg ggcagatgga gaaaggctgt tgacttgccc gttacggcgg ataccagtat
    13741 taatgcagta aaaataatga aaagcctggc cccgctgtca ataacaaagg gctttttcat
    13801 acttgacaaa aactacaaca tttcaaggcc tgcagagcat atatcaagga gtattcctga
    13861 ggataaaccg aaggaaaaag ctcaggctgc ttccattcct gatatgaacg attatttaag
    13921 acagattctt gatgaaataa gagacctctc ggataatgtg aagtacctga acagcaggat
    13981 aggaagcagt gataacagga attcaaaaga tcccaccccc ccaaagtcag gtactgttga
    14041 taaaactgca acggaacagc cgacccttaa gaaaagcggc ttcaaggaaa agttcatgaa
    14101 aaatgaaagg cttcccaact acgaaagctt attgatgaag caggagaata taaccaccta
    14161 cgtaggaaag cccggcaccg tcaaccccgc ttccgatatt tcaaaagaca gcgagaaaca
    14221 agccttcgct tcccccaata cggcagcacc aaataataaa gaaaactgga cggaggatcc
    14281 ggaagagatc ccatataaat ataacagcct tatgaaaaaa ataggagaat tctttaaata
    14341 gaattctcct tactgtcata tttcgtactc cccggtgaat acttcttttg cttcaccggt
    14401 catttgcacg tgttccccgg ccttccattc aacagtcaaa tccccgcctc gaagatgcac
    14461 cctgacaata ctcccaagct tcccgcttgc aattcctgca acaaccgaag cacaggcacc
    14521 cgtgccgcat gccattgtat agcctgctcc tctttcccat gtacgaagga ttatttcatt
    14581 cctgtttatt attgaaacaa aattcacatt tgtatttcgt gggaaataat cagatacttc
    14641 aatttttctt ccttccgaaa tcaccttttc atcctcgata ctgtccgtaa aaacaactgt
    14701 gtgaggaaca cccataaaca ttgaagtaat gagatattct tttccgtcaa cccggatggg
    14761 ataatcaact actttatcca agtcagcatt aaacgggata tccttgctgc caaacttcgg
    14821 cattcccata tccacggtta cctctttcac tgctccgtcc tcaatattca gcctgggtga
    14881 tatggtaccg gccaatgtct ccacagtaaa gctctccttg tcaaccagtt tcttctcata
    14941 gcagaacttt gcaaagcatc gtgaagcatt tccgcacatt tctgccatgc ttccgtcaga
    15001 attgaatatt tccattctga tatcgcagct ttcacttggg gatgcaagta caataccgtc
    15061 cgcgcctatg ccgaagtacc tgtcgcacat tttcctggca aggctgccgt caatatggat
    15121 attgcttctc aaattattaa tcagtataaa atcatttccc agtcccgtgt atttgtaaaa
    15181 cttcattata ttgcctccga acccggtacg gcatcactta aaattaccgc acttattctg
    15241 taaattgccc ttgagcctct cctttaatta ccttagccaa ccactctttt ccctcaacga
    15301 gcctcgttac catcattgca gctacactgt ctccgcatgc gttaaccatt gtagcgggag
    15361 ggtcaaccaa aaatccgatt gtggcaataa tcggaaaagc ttcaggcgga aaaccataaa
    15421 gatttactat aagcatttct ccgatcaatc cgcctccggg tacccccgac attacaacac
    15481 cgctcattac tgatatcaat attgcagttg caaaggtccc tattcccgtg aattcttttc
    15541 cgtaaagtcc gaacaaaaag gatatcttca aaattgccga taagcacgaa ccgtccatat
    15601 gcattgtagc acccatcgga aggactattt ccctgatatc cttcggaaca cccatttttc
    15661 ttgctgattc aagattaacc ggtaaggttg caatgctgga ttgggtcgct aaagaggtaa
    15721 atgcaggtga tacaatattt ttgaagaatc tctttaccgc cggcattcct cccgaataat
    15781 atgcgtatac gaagaaagct ataaagaaat aagctattgt aacaggataa tatatagcca
    15841 tagccctggc gtagtccttt agaagctccg gcccgaactc ccctacaaga gccgcaaaat
    15901 atgcagccag tccaatagga gcatagtaca ttataaagct taccattttc atcatgacat
    15961 ttgaaagtgt atccaatgcc tccgcaacct tcttgccttt ttcacccaag gcagttacgc
    16021 agaacccgaa cagcactgaa aatactataa gcggcaacat attccttctt gataaaagct
    16081 ccggaaagtc tgataccgtg atagccttta ctgcttggtt tgcaaggctg atcttctcta
    16141 ctggctcctc agcattgacg tttagattaa ctccttgtgc aggtggtata actgtcacga
    16201 aaaatatcat tataatagct gaaattatac cggtaacaac aaataccaaa agcagattct
    16261 tcaaaatgct gcccaatctt ctcatatttg ccatatttgc tactgcactg gatattgtaa
    16321 taaaaacaag gggtacaaca acagtgaaca tgagattcag gaaaatatcc cctaatggct
    16381 tgaaaactgc agcatccgcg cccatagcaa tacctatgat actgccaata attattgcgg
    16441 taaacagaat tatcgggaaa cgatatgcct cccaaaccgt ttgtgccctc gtactcacac
    16501 aaatcccctc tttcttataa tattttatga aattttatgc atttaacact cattcctcaa
    16561 caggtcctca taggtctgcc gctttacaat aagccttggc tttcctttgc tcagcataac
    16621 aacggccggt ttgggattcc tgttatagtt gcttgccata gagtagccgt aagcacctgt
    16681 tgatttaacg gcaagaatat ctcccggctg aagctcggga acatataaat cccatataag
    16741 tatatctccg ctttcacagc atttgcctgc aattgtaaca cgctctttct tgggggaatc
    16801 tgctttattt gctatgattc catcgtattt agcctgataa agtgccggtc tcgggttatc
    16861 cgggaatccc ccgtctatcc ccgcataagt tctgaccccc ggtatttttt taatgctgcc
    16921 tattgtgtaa agagtaatac ctgcttctcc gactatccag cgtccgggct caataaaaat
    16981 tctcggcctt tttataccgt tattactgca gaactcctca atactattca tcataggatc
    17041 aacaaaatat gacagcttcc ttttttccgt atcgtccaga tactttatcc caaagcctcc
    17101 tcccagattg aattcttgtg caacataccc gacttcatct tttatcttct ttatgaggtt
    17161 cagcataata ccaacagcca tgatatatga ctcattctcc agaatctgtg acccaatatg
    17221 gaagtgcagc ccttttaagg ttataccttc tgattccagt gaaagcttta cggcttcgtt
    17281 tacatgaggt atcggaattc cgaactttga gtcaatattg cctgtcaata tatacttatg
    17341 agtatgggaa tccactcccg gagtaatacg taaaagtata tccgccttta cgcccatttc
    17401 ctttgcaagc ctgtcaatca actgaatctc ccagatactg tcacatacaa aacgtccgat
    17461 tttgcattca agtgccgttt tgatttcttc gggagttttg ttgtttccgt gaaacattat
    17521 tttctccatt ggaaaatcca ctgattttgc cgtatacagt tctcctcccg aaacaacgtc
    17581 cagtcccatg ttttctctct tgattatcct tgccatttcc ctggtcaaaa atgcctttga
    17641 tgcataatac gcttgtacgt cctgatactt ttcagtaaaa gaatacctga tttcattcag
    17701 tctctccatt ataatatcct cagacattac ataaagagga gtgccatatt cttttgccaa
    17761 tgctacagta tcacaatccg aaaaatagta gttcattact aacaccttct tttataaaag
    17821 ttgctgcata taaaagtttg atcccgatta gcatgaactg aaaaattgtg acctatgcac
    17881 ggcttcctgt taagatcatg atttaaaatc attttaattc ttcgattgca taaaaacaat
    17941 atccaaaagt gttatcttaa cagaagcact ttttgccaca actctacatt ttaggataga
    18001 catcagctaa taatatttcg gatatcctaa aatgttttcc ttgattcata gaatacgcca
    18061 aaggatgtac attattcctg attagctgta tactggcgta ttctatatat catttcagac
    18121 atgcctgatt atctgatcct tctattttta agcttgggat cttttatttt actttcatat
    18181 ccgttgtctg acggaaaata gtattgcgcg tctttgattt catcgggcaa atactgctgt
    18241 tccacaaaat tgccctcata atcgtggggg tacttgtact caaccccgta tccccaatcc
    18301 cttgcacctt tgaatgcatc atttctcagg tgcagaggca cagccccggt tcttttgttt
    18361 tccacatcct tcattgcctt tccgattcca accactgcgg aattgctttt cggtgcgcaa
    18421 gccacgtaca aagctgcctg ggacaaaata atctgtgctt ccggcatacc aatcatatga
    18481 actgcattgg cagccgccgt tgctactatt atcgcgtttg gatcagcatt tcccacatct
    18541 tcggaggcac aaatcattat ccttcttgca atgaacttgg gatcctcacc tgcataaagc
    18601 attttggcaa gataatgaag tacggcgtca ggatcagaac ctctcatgct ttttataaat
    18661 gccgatatgg tatcgtaatg gttctcaccc ttcttttcat aatttacggc tcttttctgt
    18721 atgcagtcct ccgctgtctt caagtctata tgaatcttgc cgtcactgtc cctttcagtg
    18781 gtaagggccg ccagttccac cgcattcaaa gcagccctgg catcaccgtt ggctatattg
    18841 actatatgag ccattgcttc ctcggttatc tcaatattat aagagccaag ccctttctct
    18901 tcatcagtca ggcatctttc aaccagtttt ctgatatcct tagctttaag aggattgagc
    18961 ctgaatatag tggaccgcga aataagcgca tcattcacct cataatacgg attctctgtt
    19021 gtagctccta ttaatatgac agtaccgtcc tcaacatacg gaagcattgc atcctgttgg
    19081 gctttgttga aacggtgaat ctcatcaata aatagtattg tcctttgacc atacatcccc
    19141 ttgcggtttt ttgcctcctc aactaccctt cgtatgtctc ccactcccga agtcaccgca
    19201 ttaagctgct caaaatgaga tttgcttgta tttgctatta tctttgccaa ggtagttttt
    19261 cctgttcccg gcggcccgaa aaaaataaga gaagtgagct tgtcagcctt tatagcacga
    19321 taaagcagct tccccggagc cacaatatcc tcctgaccga caaattcgga aagatcactc
    19381 ggcctcatcc taagagcaag agggctctct ttcttcatct cctgctccct gttcatatca
    19441 aaaaaactgt acatatcttt cttcataagt atttacctgc tttctcaaca caaccattct
    19501 tatggaaaag gcaatgggct attcttgctc ccatcgccta ttgaatcaca tttgccatta
    19561 atccctgaac cgactagaat tgtactttca cattctgtct ttctgattca tcagctttga
    19621 aatagggttc ctccctgaag ccgcacaagc cggcaatgat atttgccggt acaacctgta
    19681 tagcgttatt gtacataaga accgtatcat tatagaactg ccttgcataa gcaatcttat
    19741 cctccagctc ggacagctcc ccctgaagtt ccatgaagtt tgtattggcc ttcagatccg
    19801 gatagctttc tgccactgca aacaatcggc ccaatgcccc tgtcatttcc gtgtttgctt
    19861 tcatcatttc ttccggggta cctgcgctta tgtactggtt ccttgctttt acaacagcct
    19921 ccaaggtctc tgattcatgt tttgcatagc cctttaccgt ctcaacaagg tttggtacga
    19981 ggtcaaacct tttcttcaat tgaacatcta tctgagccca tgaatttttt accctgttcc
    20041 tttttactac caaactgttg tatgtgctga ttgcaattaa aacaagcagc agcagtacac
    20101 caaatattaa tattttcata aaatttcatc ctccttttaa actttctgaa acgaaaaacc
    20161 acggcttaga atgcgccggc tccgccaccg ccgccgcctc ctcctccgcc ggaaaagccg
    20221 ccgcctcctc cgctgccgga ggaagccttt gacattgctt gcctgtagac ggattctgta
    20281 gtcttaacca ttgagtcagt tatattatct attgtatcaa agtaattgtg aatgtgtcca
    20341 tagcgtccgt agtacaaata tgtcaaatgg ctgttattaa agtcttcctc tctgaatacc
    20401 agcctgagct ggcttatgac ctctttggca actccaaggg atatggcata cacaaggtaa
    20461 tgctcccaca tgattactgc aggcaaatcc gccttatcaa ggctgctgaa gtgcctgagg
    20521 aattttctga aagctttcca catcttgaac tgctctacgc cggatttcga ccttctcctt
    20581 attgtcaaag agtatatcag cagtataatc gataccagca tcaatattgc aaatccaaga
    20641 atattatcat gtcttgccgc agtatatccg ccgaaaatca taccgataac agctgttaaa
    20701 acaccgaaga gtatgccttt aactgtgttt ttgtcaaaaa aggaataact gtctgcttcg
    20761 gattttacaa gtccggtcca aatatcgtaa tctctgctga aatctattgc actttctcgt
    20821 gtcttagaag aattctcaat atcctcaaga gatacccgtt ctccgtcccc tattctaaat
    20881 atcagccatt ctatggcata tttctcatga ggggaaagaa ccatcaaatc agcttccttg
    20941 tttaacttga atatatactc gttatcagct ctgctgccga agagtccgtt aacctcttcc
    21001 ttttcaacaa taagctccag gtacttcatt ctgaccaagt ccattaatgt agcggtcaag
    21061 tctcttggct tcacactgcc aaagttccac agcactgcca taactgcggg ggaatagctc
    21121 ccgggaagct ccctgaaata atctcccgcg aattttgcct tgtattcctt atcaaatttg
    21181 aaatacaaat aaacaatcat caatagctca aacaatacat atacaaagga aaacccgaca
    21241 attatccttg cctttgtcct tatctcattt gcctcatcgg caaattcctt ttcctcagac
    21301 attatctccg caagggcatc cctattaaat accttttttg aatctttaat caattcaggc
    21361 ggaaaaagta ctcttgcttc gacaaagttt cgagccggca attcatccac tctcaaaact
    21421 acggtttttg aatccgcgat ttcggaaaaa ccggataatg gcccatgccc gaatactttt
    21481 atctgctctt tgtcagcacc ttccggaatt ttaattacaa gctcaaagtt ctctatttta
    21541 acttccgtat cttcgcccat gaatttccaa tagatttccg caatatcatt aaactttgcg
    21601 gcagcgttta aaactctgta ttttaatata aatgtcctgc tttcatttac agcagcagag
    21661 aatatcttaa gctttatgcc gccgtcttca ccaatcagtt gaaatgtatt atccctttcc
    21721 gagttatcct gtacaaacgg agaaagcata tcaggctgct tcttgtatac ctctacagcc
    21781 tctataccat cgctgcctgc tgtccggagg gttctgtaca cgcccctgaa gcttccgtca
    21841 aactcataag tgatttcttc aatgatatcc atattcccca tactgtccaa ctgggcattt
    21901 atcttgaagc tgctcatata atagcttctg tcgtcacaat atgcagttat cacggataaa
    21961 agcgcacatg tgatgagaaa tatgagtaat atggtaattc tccttctcaa atccgacctc
    22021 acctcctaga caacattgta taacaattag tggaaaagta aaagaaaaaa gatgaaggac
    22081 acggggacgt ttctcctgtc ccgctttcag cgggacagga gaaacgtccc cgtgtccttt
    22141 atggcgaccc tgctattcta ttatcttttc cacatattcc gaagtaaagg ctgcaagcaa
    22201 tgcgccgtag cccagatcaa actccatgat gtctcctacc ttgtattccc tgccgcagtc
    22261 acttacatca attattaaat ggtcgctgct tgctcctatt actatggcat ccttgtctct
    22321 gggctgtatg ccgtccgcct ttaagtcctg cctgcctgaa gccaggatag ctcttttcct
    22381 tatgcctctg tcgacaaaca caggtttttc gccgaaggcg tcaaccccgg tctctccaac
    22441 aggtacggag ggcttttcct ttagctctat tatttccgcc atgtatgtga acacatctcc
    22501 gtaggtgcct tctattcttt ccccgtaagc agtctcattt ccaagaacaa ttgcttctcc
    22561 aaggcgcagc tggtttatac ctttggggac ttcattcttt tccaccaggt gcaagctgct
    22621 tgaattcccg ccggagatta tatccagctt aatatcatat ttcttttcaa tctcttctgc
    22681 cagggctgcc agcagcccga ggttctccac gcttgggatt actgctccgt agcaggtgag
    22741 gtttgtgccc agccccacaa gctttatacc cggcagtttc actattctgc cggcaaattt
    22801 aatcgcatct ttataccata cgccttccct gaggtctcca aggtctgcca taagtatgat
    22861 tttatgaacc ttgccccttt tctgtgccct atcggaaagg gcttttataa cctcatactc
    22921 tgagttaagg ctgatatcaa catgttcaac tatttcgtca acctgtgaca gcatcggaat
    22981 tctgagaagc attttttccg cattcagctc tttcagcttt ttaatgttct caatccttga
    23041 atcggcaagc atttttgccc ctgcagccac ggatgcttcg gcaatctccg gcataccgca
    23101 gtataccttg gttacgggta ctatgtcaat cccaagcctt ccgcactttt ctgccagtac
    23161 tttggtattg tgcttaagct tttctttgtt gacttttatt gcaggataac tcatttttca
    23221 attcaccccc aaactttcct tcataagtgc cagtgccgct tcgggaaacc tctttgaaag
    23281 cactcccagg gatgccatta tgtaatggtt gtcaatcagc acctttgctt cctttcccgg
    23341 gtacaattca gtccctttgc cctgcagccc cacggatttc aatatgtcaa tacgcccggg
    23401 gagcctggtc agcgcccctc ccgttccgat tatatattta acatttgtca ggtcctttcc
    23461 ttctgcaact gtcttctttc ctgtcgggcc gtaaaggaac cgcagttttc ctgcatgcct
    23521 ctcaagggat gtaagaacag cttccttggt cagtctttct acaaaagcct tttcccgttc
    23581 ggtttcggga atcggttttg cattatttac aagctcttct gcctccggaa attccttctt
    23641 aagattttcc aatccaatct tttcaactat atgcggaata ttcacatgta ctcccaaatc
    23701 tccctcaaca gtcctctttg ccgcaggttc agggcttata agcataaggc ttatttcttc
    23761 ggagccctct gtgactgaat gaacatccgt agtggcgcct cccacatcga aaaccacaag
    23821 gtcgcccaaa tgttccttca atagcctggc agcctccatt accgctcccg gcgttggaat
    23881 gatggggcct ctgaccatat ctctcacagt gctcattccg ggagcatgta ttatgtgctc
    23941 ttcaaatacc tcctgaatga ctttccttgt tggttctaca ttaagcatat caaccttcgg
    24001 atatacgttc tccactatat aaagccttgt atttgtctct tccgcaataa gccttacttc
    24061 ctcctgattc tcgatattcc cggcatatat taccggaatt cctaatccca tggaagcaat
    24121 catttcaaaa ttgtgcaggg cggtatcacg ctctccgtaa tccacccctc ccgcaaccag
    24181 tattatattc ggccttacat cttccagcct cttcaaatcc gttctcctga gcctcccggc
    24241 agtaacatgc tttattattg ctcctgcacc cagggcggct tcctttgcag ctctgaccgt
    24301 catatcgtaa acaagtccgt gaaccgtcat tctgaggccg ccggcagcac tgctggttgc
    24361 aaggaattca ttccattcaa gctcaggtgc tccaaggttc ctcttaaggt catccactgc
    24421 acctttaagc cctatagtaa catcaccatt atacaccgaa gtaggagctt gcccttgtcc
    24481 gaggaattta ggacaagggc tcataatatt gtcaaatgca ttgaccactg tagttgtact
    24541 gcctatctct gcaacaagca cactaatttt cattgctgtg cttttccctt cttctcttga
    24601 caaggtagct tgctacatca attccgtggg tacctctgcc gaagcccgca tccattcccg
    24661 cttcaacagc tatttcattt gtgacctgtg ttccgcccac aacaagcatc actttatctc
    24721 ttataccctt ttcaacacaa agatcgtgaa tctttcgcat attctttgta tggacatcgt
    24781 catgggtaat tatcgtgctg gcgagtattg catccgcatt tgtctctata gctgcatcta
    24841 caagtttttc aacagggcag gaggttccaa ggtacaggta cttgatacca aaaccttcta
    24901 tccctccatg ctttatatcc agagtctcct tcatccctac agaatgctca tcattcccta
    24961 ttgttgcagc tactacaaac attggttttg ctgagatatc ttcccttatt tcctcttctg
    25021 ataatgtttg aaccttttca ggtattacaa gctcattaac attaatgtca aatataactt
    25081 ttcctttaat ttcaactaag gtaccttccg acggatgcag cgcctgcttg tgcactacag
    25141 tgacatcttc aaggcccagt ttctctccgc attttattgc tgcaaattct gcagttcttt
    25201 catccagtgg gaggaatatg gtagttgcta caacaccgtc agctctccat tcaacctcag
    25261 gttttacagt attcgtatta taatattttt ccagatcagc aagccttgta ttcacattat
    25321 catactcatc aagctcatct atatagataa tcttcgaagg atcttcaaag gtatcattgc
    25381 ctatggcatc tgatgccttc ttaaattccg aaggtatgtt attatatccg tagtgaacag
    25441 atacaggagc aaagtagtcc ttatcccttt caaatatcat tcccacacct attccgccat
    25501 caatctgtct gggaataccg tcaccatttc tttccggata atatccgctg tcgatgaaga
    25561 atccattttc cacagcctta aaatatccgc ctgtttccaa tatctcttcc atgaagagga
    25621 ctgctctttc tttaagctcc cttacttcct ttcccaaagg gccttccctg tccaatttaa
    25681 ccatctctcg aaggccatcc attccattaa gagcttgttt agctgtattg accgcagcta
    25741 tgttattgta atgccacggt acattccttc cttcatcggg agtgatggtt gactgtatat
    25801 cggcacttgt aagtcttgaa atcaataaat caagagtatg tgtaaccgtt gcttctctca
    25861 tgtctgattc catatatttt gtgttctgct gagctctcat tttgtactct gagaagaagt
    25921 cccttagtgc aaccgcataa ggcaggtcaa tcctcatacg cggcgccggc ggcgccgtgg
    25981 gcggcactgt tgatagggct atgttttctg gtttaatacc aaccttccgt gagaatatac
    26041 tgtttatggc atgctgaacc ataagctcag gcctgacctt ccatgccttc attgcagtag
    26101 cattggcatt atgggccccg tctatctgca gaatatccgc ccatgccatg accttcttcg
    26161 cctcgcaggc atctacaaag cttcttacca tgtttacatt tctgtacaat acgttgtatt
    26221 gaggatcctg atgagcgcca tttactcctt cttccgcgaa cataacagca atatcaggtc
    26281 ccgcgacacc ggatatatat gaatggaaat taatcggcct tcctacttca tcctctatca
    26341 tatcgagagc ttttcttgtt gccctgcact gctttcttgt aacggctata ccacctattc
    26401 cctgtgtagt tccctcaatc aatgagtcaa tatgagattg ccccattgtt ctgataacca
    26461 taatatggtc agccccatgc catgccgcca ttctcattct tctgatatca tcttcaaatc
    26521 ttccggaagc aatttctgta gtaactacac aatccggctg cggatcaatg taattgaatc
    26581 ctcggcttcc cggcaaggga atgtagttct ttagaccttc cgaggtttca tggtattcaa
    26641 aatcccctat cttcctattt ttatttctgc cttctctcca gtgccagcct cttcttctcg
    26701 gtctgtagct ttcaaggtcc tgcaataatt cctttatatc cagcttttta ttcggatcca
    26761 gtttcatttg ctccccctcc tttaaaacaa tgcgtcgacg tcgtcccagt attttccttc
    26821 tgcaagtccg actcctgctt ctctcacaga gacccccagc ctctttgaaa gcctccatac
    26881 tacattaccg gcacccttgc ccataagtcc ttttctcatt acgccttcaa ctataggctt
    26941 tacttcaaga cttgagaagc ccatcctgag aagtatgctt ctttctattg cgggagttgt
    27001 gttcttataa ccaagagcca gcatcggatc aactattttc tcagccagct cccagaacct
    27061 ctttttcagc tcttcgtcag aaagctctgc caggtgcttt cttcttattt catagtcatc
    27121 tgcacgtttc actgccattt ctatccctcc atctacttta ttttcacgtt tagttcagca
    27181 agtacgcctt ccacaaaact gtcatcagtt tttgcatccc ttacaaggaa ttcaaaatcc
    27241 tcttttgtaa tttcagttac cttgcaatta ttgatgcagt ttttgatata tgatgctctt
    27301 gccttgtcta tatcgtaatc tcttactttt atatctgacg gatgtgccgg aagtataata
    27361 ttcttcccgg gttcgtcacg gtcgggattg ccgaacaata tttctatacc gttttgcttt
    27421 gcaaatgtaa gctgcggcag cggatgcttc cctgctcccg tatattctgt ttcctgaacc
    27481 acaataatct ggtccttatc catttcttgc gccagggcaa aagcagctgt taaggatgtg
    27541 tttccggcag gccctctctc cagtccttca agcactgata atgcttctgt aatatagaat
    27601 acttcacctt gttttaccag aacatatctg tcaagatatc ttaaaggcct tgcagcgttt
    27661 ctcggtacat ctgccctgtc aggccatgtg gcaaagggta ttccgaagcc tgtgtggcct
    27721 gtagtaaagg attttctatt gaaatccctg tcggaggcca tatgaagacc cgaaaggtca
    27781 acgcttgcac cgacaacctc aacatccttt gctcctgctt tcaaaagccc tcttgctgta
    27841 cctgttacat ttccgccccc tgcgtgtgta attacaacct tgtcgggatc tcttccaatc
    27901 ttctccctga actgcatggc aagctcatag cccagtgtct ctacccctgc tattccaaaa
    27961 ggtgtatata gggatgcatt aaagtagcct gtctcctcca gcaaggtcag gaaggtatag
    28021 aagagctccg gtcctactgt cagctgtacc acctctgctc cataggcttc acaggcacgc
    28081 tgcttttcaa gtatttcggg ctgtcccacc atcctgctgt cgtaacattc ctgaacaaca
    28141 atgcatttaa gccccctctt tgcagcctgt gatgccaccg ctgcaccata gttgccgctt
    28201 gttgccgtta ccactccttt gtatccgtgc ttttttgcat gatatatagc agtagccgcc
    28261 cttctggcct taaagcttcc ggacgggttt gcagcctcat cctttataaa aatccttgcg
    28321 ccttttccct ttggagcaag ttttcgggcc aatgcagtaa gatttttcag ctcaacaaca
    28381 ggtgtgttcc ctacgcttgt ctcagcctgt atctgctgca tttcctccag cgtataacct
    28441 gcctctctca tcattctttc gtaatcgaag gatattcctc ctgattcaaa tacgctatag
    28501 tcaataccaa ttgcctgttt cattatttca ttttttcttg ccataaccgc tgcgtatgac
    28561 atatccttac tcatttgaat cacctccgaa gaggatttcc ttaagctgca tatccacctt
    28621 taaaagctca ggtacgaaat cgccatagtc atgtatatac ctgggattaa ctgcggtcag
    28681 ctcgcctctg accttcctgc cggtggcagt tacgatttca accgtcgaac ctatttttgc
    28741 atcctcttgt gctataccct ttacccataa ctcaagaggc atcttcttgg tgtcctccgg
    28801 aacctgcggc gccctttctt caggtttcag aacaacctgg cgtatctgca cccagtctcc
    28861 tttttttgcc gagatcattt aacaacactc ctctttatca ctattaaaaa tgcgtctcac
    28921 gacgcgtttt tttttgctag ggttttaaat aagtaccctt cgtttttgct ccgcatcctg
    28981 aaactccttt atcggcaggg gcttcttccc cttgcatccc ccattgtata ggaaagtata
    29041 ctttcctata caataaaaaa tcttttcgcc ccgtaataag gcaaaaggga catatttgta
    29101 ttttaataat aaatatatgt cccttgtttt aactgctatt cttctatcat ttctctcata
    29161 tctcccataa tagctctggg cacagggagg tcaatcatgg tcttaagacc ggcccttgca
    29221 ttgatgacat gaggaatcat attgacacac attgcgattg taccgatacc tccgggtatt
    29281 tccggcttgt ttgccatatt tacatttggt gtgcctttga taataatata atctccggtt
    29341 tcaactcctc caagatgagg ttcaatctgc tgcggatgat ccatttcaat tttaagctct
    29401 ccgtcaacgt atccatagcc cttcatggca cagcccgcaa catctcccgc cttggcaaat
    29461 ccataaggtg acttcctgtc aacttcggtt acaatcggct ccatgctctg gttgaagtca
    29521 ctgagcttcc agccaatggc atccgctatc atacctatgg attctgcaaa acctacatgg
    29581 cctgccagct ttcctgtcct gactccttcc ttgaattcat caactgtaat accaattccc
    29641 tgctcttcca ttactgccgg cccgaaagga gacaagctgt ttacacgtct tgcaacaata
    29701 tgctctactt cttcacaggc acctgtaaat gcaacaacaa gaagatccat aataagacct
    29761 ggatttatgc ctgttcccag cacagtaacg ccattagcct ttgctatctc gtccagttgt
    29821 tttgtaagtt ccggttcagc agcttttgga tatgccattt gctctgcact ggatattaca
    29881 tttatctttt tctccagaca gtactttatt ttgtcaaaaa cacctttagt aaatgaatcc
    29941 gtacaaatca gcacaacatc tgcagcattc tctttaataa catcctcata ggatccgatt
    30001 attacgtcct tattgctgcc tctttcagtc tttatgtaat catacatact tttaccgatt
    30061 tttgctcctc ttccaacggc accaactatt tccacgccct tctttttgag aagcatatca
    30121 gccattccgc cgcccattgc acctaatccc cagataatta cctttacatt ctcttttctc
    30181 attaagcaaa cctcctaata ataattagag cactgcaaat tattgtaagc aaacctcatg
    30241 ccaaatgagc cattaagaca gtagataaaa aaatattttt aagtgttgat attactgcct
    30301 aattgatgcc ttaccactta attgcatatt tcagaattat gaaacatacc atatccgcgc
    30361 aaatttattt gcatctctgc caatttcttt gcactccgat tgagtttttg ggaaggctat
    30421 tccttcaatc tgcttgtttt aaaaccaata atttagatat atgcaaattt ttttgcatta
    30481 atattgcatg tattctcatg gcttgcattg actttcatta ttataaacct taaaatggat
    30541 tatatgctct acgataaata aacgcacttt ataaggagat attgccatgt atgattttca
    30601 cgtacattct cacttttcct ctgattcaac cgcatccatg caaagcatgg cagcgaaggc
    30661 aaaatgcata ggccttaagg gcatctgctt tacagaccat tatgatttgg aatatcaaag
    30721 caccgaaatc agctttgaat tttcctatga tagatatttt gatgagatta acagcctcaa
    30781 ggcaaattcc tctgaaaatc ttgaaatata caccggactt gagttgggga tgcagcctca
    30841 cctgagtgct gagaatgaag ctcttcttaa aggcaggagc tttgatttta taataggttc
    30901 catccattgt gtgaataaga aggacatata ctgcaggact tttcttgaag gcattactga
    30961 aaatgaagga ataagaaact actttgatga tatgctgcag tgtattgagg aattcaagaa
    31021 ttttgacatt ctggggcatt tggatgcagt aagaagatac ctttacaata aggataacag
    31081 tttctcattg gacctgtatg aagaatccgt caggaatgtg ctgtccaagc ttgtgcattc
    31141 aaacaaagga atagaagtaa atactgcagg ccttcgttac tgcctctctt cctttcatcc
    31201 gctgccggat attctcaagc tgtaccgttt gctgggaggc gaaatagtga ccgtaggttc
    31261 cgatgctcac aaacctgatg acttgggcta tggaataaaa aatgcattat ccgtcctttc
    31321 ggaacttgga ttcaaatatt atgcaatata taaggacaga aagcccatat ttataaagat
    31381 ataggaggca gaagcctcct atcactatca gtttgcagac aaagttcatg taaagcagta
    31441 gggacggttc ttttttaaag cagtagggac ggttcttttt gcttcaaatg caaagcaaaa
    31501 agaaccgtcc ctactgcttc aaagcaaaaa gaaccgtccc tactgctttg cctctctttc
    31561 gtaccttttt gcctcctatt cctttatatt aatatcatat ttcttcagct tgtgctgaag
    31621 ggtttggcgc tttagtccaa gcatttcggc gcttctggat atattgtttt cacacttttg
    31681 cagtgcctcc agaatcaacc ccttttccac ctctgcaact atcctgtcca ggggctggtc
    31741 ttcattcaaa ttcaggtaat ggttgttttt gccggccttg aacattactt cacacatttc
    31801 ctgggagata tgttccggct tgattacgtg ccctctgttg accatattta ttgcactttc
    31861 aattatattc tccaattccc tgacattgcc cggccaatta taattcagga aggcctcctt
    31921 tacctcattg gaaacatacc agatatcctt ctgaagtata ttgttatact tcttgataaa
    31981 atggtctgtc agaagcataa tatctgtttt tctttccctc aggggaggta tgctgacaga
    32041 tatgacatta agccgataat acaaatcctt cctgagaatt cctttttgaa gcagtaccaa
    32101 agggtcctca ttggtagttg ccagtatcct tacatcaacc ggaatatccg aaagcccccc
    32161 tattctccta atgttgccct cctgcagcac ccttaaaagc ttcgcttgaa gctgatggcc
    32221 catggaattt atttcatcca gcatgagggt tcctccattt gcctgttcaa acagtcccgg
    32281 cctgttgata gctcctgtaa agcttccttt aactgttccg aacagtattc cctccagaag
    32341 cgactccggc agtgcagcac agttttgtgc tatgagcggc ttatgcttcc tgctgctttc
    32401 attatgtatg ctctgggcga atagttcctt tccggtaccc gtctccccgt atattaagac
    32461 tgtggatgat gacatagctg ttttcctggc tatttccacg gcattaagga atttttcgtc
    32521 ttccccgatt aaatttctga aggtataccc ttttaggctg ctgccatcaa tatctttgct
    32581 tgcaagctcc tgatgcaggt atacaagctt atcggacagt tccttctctt ttgttatgtt
    32641 ctttgcaatt tcaagggctc cgatcttgcc ctgagatatc ctgataggta tagtgctgtt
    32701 taaagtgctt atctgcttgc ctttattgtt cagatatgtc tgatatttgt tgtatattgg
    32761 ctttcccatt ttcagcactg tcagaagcgt actggtctca gtggtcaggc tggggaaaat
    32821 gtccagtatg cttttgccca tgacctcatt tatatccagg ccttccatat cggccatagc
    32881 ctgattgtaa ataattgtct tccctgagct gtcaaccaca tgaataccga tatctatgtt
    32941 ttttagtatt gtatcaataa ttgacttata aattaaactc atcgtcatat cattacctca
    33001 catgcaaata caataaaaag gtatgccaat acataatata tattggtcat accttattat
    33061 atcataaaat ttgcatgaat ttatggttta ttcaaactcc ggataaagag gatgcttgcc
    33121 gcatagcgcc ttcaccctgc tgatgcattc ggcctttgat tccggggctg acagtgcggc
    33181 tgatattatt tcggcaattt ccttcatctc atcttcctta aaacctcttg tggtcactgc
    33241 cggagtaccg atcctcaatc cgctggtgat gaaaggactc tgaggatcaa agggaatggt
    33301 attcttgttc gttgtaatcc cgatatcatc aagcaacctc tccgcatccc tgccggttat
    33361 gccttttctt ctaaggtcaa gaagcatcat atgattgtca gtgccttttg agacaatgtc
    33421 aatatccttg gctgtcaacg aatctgccaa aaccgaagca ttctttagca cctgctctat
    33481 gtatcccctg aattcttcgc tcattgcctc tctgaagcat acagctttgg ctgcaattac
    33541 gtgcataaga ggccctcctt gcattccggg aaaaaccgat ttgtctatag ccttcgcata
    33601 ctgttgtttg catagtatcg caccgcccct ggggcctctc agtgtcttat gagtcgttgt
    33661 agtggcaaag tcggcataag gcacggggct ttgatgtaat ccggctgcaa tgattcccgc
    33721 aatatgcgcc atatctacca ttaagagtgc tccgacctcg tctgctatat ctctgaaagc
    33781 tttaaaatcc aatcgccttg aataggcact tgctcctgcg acaatcaact taggccggca
    33841 ttcttttgca atagccctga cattatcata atcgatcatc tctgtgtttt gtgacactcc
    33901 ataatgaatg aacttaaaaa acttgccgga cacgttgaca ggactgccgt gagttaaatg
    33961 tcccccgtgg gatagatcca ttcccattac cgtatcaccg ggctccagta cggtaaaata
    34021 aactgcttgg ttcgcacttg aacctgaatg aggctgtaca tttgcatgtt ccgctccaaa
    34081 caaggctttc atccgttccc ttgcaatatc ctcaacaata tctacatact cacaaccgcc
    34141 gtaatatctc tttccggggt atccctccgc atatttattc gtaagcactg tacccatcgc
    34201 ttccaataca gccggcgata caaaattttc tgaagctata agctctattt tattcctctc
    34261 tctgtcaagt tcctttataa ttgcttcggc tatttcgcta tcacaagctt tgattctgtc
    34321 aattttcata aaaacttcct cctgtggtgt tttatactat ttactatata tcataacaaa
    34381 gatacgtgct taattgacaa ataccctctt actttaacat tacattttct aatgaaaata
    34441 ttagaagcgc ttatagatgc tataagcgct tcgttttaaa tgtgcttttt cagcatatca
    34501 tcaaaggcct gcttgggcct tgccccgata actccgtcta ccttgctgcc tttatcaaac
    34561 agaaatactg agggtatgct cattatatcg tacctgcctg caagttcggg gttttcatca
    34621 acattaacct ttgctatatc aatctttcct tcatattcat cagccagctg gtctatgacg
    34681 ggtgccacca tcctgcaagg accgcaccat gccgcccaaa aatccaccaa taccggcttg
    34741 tttgatttta acacctcact gtcaaaatta ccttgattta ctatcttaat attcttactc
    34801 acttattagt actcctttca aatctgcagc taatttttat acagattcat atacattatt
    34861 ttatccaata ggccgattat taaattaata aacaatagaa gctgttccat taaatactgt
    34921 cttttgttct tatactaaga ataacaggct tgaatttttt catttttttg atagacttat
    34981 ccttgaattt tattatcaaa taagaaacag ccaatgcagc aagtcccaga gcaacgccga
    35041 ttgcttgcgc cgaattatcc tccaaaccca gagccaaaga accgtaatac ccgccaaata
    35101 ttccggccgt cagtgccagc aaaggaaaac cgtatgcaat agttactgca gtcatgaagt
    35161 tcatgttttc aagctctatt tcaacttcat caccaacctg ggctcccaca tcattctcgg
    35221 ctgtcatcat catctcaagt ttatccttgc ctacctggca ttttccgcat tcgccgcagg
    35281 ctgtgcctct ggtaaatgaa attgttgcct gatttccttc tatctttact actttcccta
    35341 tttctctcat aagtttgtat caatcctttt acttgacata tcttactatt gtttcaatta
    35401 tttcttcaat gcttttgtca ctattctcgt ctttaaggga ttccttgaga cactccttta
    35461 tatacctgtc cataataagg ccgccgactt tgtttattgc ggatcttatg gcggaaatct
    35521 gaaccataat gtctccgcag cacttgtctt cctcaatcat cttttgtatc cctttagcct
    35581 ggccctcaat ctttttcatc cggttaagaa tgtcttcctt acttatctcc ttattatcca
    35641 attcatcgcc tcccataccc cgtaccggta ccctaattac attataatat atattaaata
    35701 tttgtcaata taattttaaa acgtataaca ctaaacacac cgaaagccac cgagcccatc
    35761 cctaatctcg cacctcgtac cccgtacctc gtacctaccc cataggaata ccctataaat
    35821 ctaatatatc ccttgccaca gccgatgcta taataaggcc tgctgccgaa ggcacaaagg
    35881 atatacttcc gggaatctgc cttctttttg tacaatttgc agtctcttta ttgctgcaaa
    35941 tgcagtttgt tttacaatcc gcaacatcct tctttggtga aatgggctcc tctttcgagt
    36001 atacaacctt caggctttta acgccccttt tgcgcagctc atatctcatt actttagcca
    36061 gcgggcacac agatgtctta tatatatcag taatcacaag ctttgtggga tcaagcttgt
    36121 tgcctgctcc catgcagctg ataaccggta tgttcatttc aatgcatttc ataacaaggc
    36181 tgatctttga gctgaccatg tcaattgcat ccacaacata gtcatactcg ggaagcagca
    36241 gcatttccga ggtttcttca ttataaagct ttttatgtac gactatatcg gcttccggat
    36301 taatatccgt gagcctttct ttcattactt ccaccttggg caggcccacc gtttttcttg
    36361 tagcatgtat ttgcctgtta atattggtaa gacagatatt atcatagtct acgagcacaa
    36421 gccttccaat tccgcacctg cacaatgcct cggcggtata ggagccaaca ccccctatcc
    36481 caaaaattgc tacggtgctt tttttaagtt tctcaatacc gtcttttccg attaacagct
    36541 ctgtccttga aaatgcattc atagattacc tccaaagggt ttgtaagaca tgggtgggac
    36601 acggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc cgtgtcccac
    36661 cgtccccgtg tccctgctat atttttacct ctatatgaag ttcttccaat tgttttggat
    36721 ctgcggctga gggagcgttt gtcataaggc agcttgctgt ctgtgtcttg gggaatgcta
    36781 ttacatctct tatattgtct gttccggtaa aaagcattac tatcctgtca aggccaaagg
    36841 ccaatcctcc gtgaggagga gtaccatact tgaatgcttc caacagatac ccgaacttgc
    36901 tccaagcctg ttcctgcgtg aagcccaaga gcttgaacat cttctcctga agctcctgcc
    36961 tgtgaatcct tatgcttcct cctccaagct cgtagccgtt taataccaca tcataagcct
    37021 ttgcccttac tttcagcggg tcggtgtcca aaagctccaa atcctcgtcc atggggcttg
    37081 taaacggatg atgcattgct acgtatctgt tttcttcttc gtcataatcc aaaagcggga
    37141 actcggttac ccacaaaagg tcatactttg tattgtcaat cagcccaaag cgttttgcca
    37201 attccagcct caattgcccc agggaatcaa aaaccacctt cggtttatca gatacaataa
    37261 aaattatatc tcctagtttc ccacccactc tgtcaattat accgttaagt tcataatcct
    37321 tcaaaaactt tgctataggc gattttacgc cctcagaagt tatattaatc catgcaaggc
    37381 ctttggctct gtatgtcttt acaaactccc caaggctgtc tatatctttg cggctgagct
    37441 ttgctccgag cccctcagca tttatagccc ttatgattcc gccatttgca cgggtctccg
    37501 taaatacctt aaactccgaa tttgcaacca ggtctcctat atctttcagc ttcagaccga
    37561 accttgtatc cggcttgtca gaaccgtaat gctccattgc ttccttgtat gtcagtctct
    37621 tgaaaggtgt ttgtatatca acgtcaagaa cttctttgaa cagtctcttt aacagccctt
    37681 cattaacctc cattacatct tccttttcaa cgaaggaaag ctccatatca atctgagtaa
    37741 actccggctg cctgtcggcc cgaaggtctt catccctgaa acacttggct atttgaaagt
    37801 atctgtcgaa gccggacacc ataagcagct gtttgaaaag ctgcggagac tgaggcagtg
    37861 caaagaaatt ccccggctgc accctgcttg gaaccaaata atccctggca ccttccggtg
    37921 atgtcctgat aagtatcggc gtttccactt ccacaaatcc gttttcatcc atataatccc
    37981 tggttacctt tgcgattttg tgcctggtga ataaaaactt ctgcatatgc gggcgcctca
    38041 agtccagata cctgtatttc agccgcagct gctcagaagc attcaggtca tcctcaatat
    38101 aaataggtgt tgtctcagcc cggctcaata tttcaaggct gctgacagct atttcgatat
    38161 agcctgtcgg catcttcgga ttaattgcat tttcatctct ttttataact tcgccggata
    38221 tcttcagcac atattcactt ctgactttaa aagcttcggc aaatacatca ggagtggcat
    38281 ccttgttgac ggtgcattgt ataataccgc tcctgtccct taactgtatg aatatgagtc
    38341 ctccaaggtc ccttctcctc tgaacccagc cggcaagtgt caccttttcc cccaagttat
    38401 ccatgtttaa ctctccgcac atatgtgttc tgttgattct tgccataatt tcatctccta
    38461 catgtttttt atatagcccg caagtgcatc aaggctgatt tcttcttcgc tgcccttatt
    38521 catatccttc accttgattt ttccgctttt tatctcttct tcccccaata ccgccacata
    38581 tcttgcatta atcttatttg catatttcat ctgggccttc aggcttctcc ccatatagtc
    38641 cttctcagcc tttatgcctt cttttctcag gaggttgact tgcctgagcg cttcttccgc
    38701 tgcttctttt cctatgtttg caatataaac atccatgtaa acaggctccg gtatttttat
    38761 tccctgattt tcaaccacaa gaagcagcct ttccaatcca agcccgaagc ctattccagg
    38821 cgtgctgggg ccgccgcatt cctctatcat cccgtcatac ctgccgcctc cgcaaactgt
    38881 gccctgagac cctatatccc tggacacgat ttcaaaggct gtcttggtat aatagtcaag
    38941 gccccttact atttgagggt caataacata ttccagtcca agagcctcca gggatctttt
    39001 caggccttca aaatgctcgc cgcattcgct gcagacatta tccagcatgt aaggaacatc
    39061 agcaagctgc tccttgcagc tttcctcctt gcagtcaatc actctcatgg ggttcttatg
    39121 gaatctgtcc ttacatgttt cacagagctt atcaattttg cccgataaat actccctcag
    39181 cttcttattg tattccgccc tgcatttggg gcatcctacg ctatttatcc ttatttccag
    39241 gtttttaact ccaagcctgc cgtacagaat catggcaata ttgattatct cggcatctac
    39301 ggaggggtcg gaactgccga aagcctcaat atcaaactga tggaactccc tgagccttcc
    39361 ggattgaggt ttttcatacc tgaaacaagc tgttgtacaa aaaactttta ccggctgaat
    39421 atcattaaac atgctgtgct ctatataagc ccttacaaat ggcgctgtcg cttccggctt
    39481 aagggttatg cttcttcctc ccttatcctt cattgtatac atctcttttt gcacaacatc
    39541 cgtagtatcc cctattcctc tttcaaaaag ctcggtatgc tcgaaaatgg gtgttctcat
    39601 ctccttatat ccgtgcaagg cgcaaatttc ccttattaag tcttctacat actgccattt
    39661 gtatgattct gacggcagta catcctttgt gcccttgggc gcttgcgtta acatataaat
    39721 cactcctccg acaatatttg ttctaaaaca tcaagcttct tccttatcat tgcttcgata
    39781 ttctcaatat aaagctttat atcctttata ttgtgctgcc tttctaacct gttttcactg
    39841 tttatgacaa ccttggtcac agcatctgct ccaaaggcaa caatggtttg cctctcttca
    39901 ataatctgca tattatatat acattcaaag cctttttttg aaaagccaat gttctcaagg
    39961 ttttgtacca tatgcttctg cctgtacagg taataaggat gcatacccat atccccaaga
    40021 aaaccttttg tatattccat catccgggat acattttcat catcgggcaa agcacttttc
    40081 atatactttt cgttcaatac cgaagccctt ttcaatgcca tagtatgcac cgtaacgctc
    40141 tcagggtcaa gcttttttat ttcttccaga gtatgagcca attccgtcat tgtttccccc
    40201 ggaaggccta atattatatc catattgata ttaccaaaac ccatttcctt tgcaagcctg
    40261 aagctctcta taacctgtcc cggatcatga gccctcccta tacgcattag ggtatcatca
    40321 ttcatggtct gcgggtttat gcttatcctg cctatattat tctcatacaa tacctgaagc
    40381 ttttcttttg ttatggaatc aggccggcca gcttcacagg tgaattcaca ttcctgcctt
    40441 ccaaagctgt tgccgatgca tttaatcagc ctttcaagct gttgtgcgtt cagggcagtg
    40501 ggagtgcctc ccccgatgta tatggtctgc accttaaagc cactggtgtt taaaaactcc
    40561 gaaacccttt tgatttcttc cgtgagtttt tccatgtagg gctctacgta tccgcgatac
    40621 gcttttattg aatttgaggt aaatgagcaa tattcgcatc tgctggggca aaagggtatt
    40681 cctatgtata tgcttatctc cctattatta taagggtata caaagggtct ttcaatatct
    40741 gcgattctga ctgccagttc agctctatcc ctgctgacaa gatattcttc tgccagcctt
    40801 tcaggtatat cggttgtaga aaccgcatta ttcataagct catgaacaat tttcaccggc
    40861 cttattccgg taagtacccc ccatgggtat tcccttttga atactgccgc cagaagcttg
    40921 tacatggatt ttttgaccag gcgcttcgct tcattgccat ccgcggccct tattccactt
    40981 ttttcaacag cttcttcatg cttttgccct tccaggtatg tagatacgca tacagcgatt
    41041 tcaccgttta tatccagcct gctggttatc aaaatgcctg tatcctgcaa aaagctttgg
    41101 ctgtcagcca aagctccctc agaccttact atctcgcact tgccgaaata cattcttact
    41161 atatcggcag cctcattata atattcatgg ttaatcagtc tgatttcaat catagcttcc
    41221 cctaaatatc atcattgtat atttgattct taacctccga aacaatacct gaagctattc
    41281 ccaattccgt ggctatttca aaatcttcca ttccggattc tatcatatca ataagctcat
    41341 tatattcctg atacatccta ttcttatgct tactggccaa gattttttta gaagacttat
    41401 tcatataaaa ccctcccatg aaacaggtat ttattatcat agtctttcct tttgccccaa
    41461 gcatattcat ttaataatca atcaaacaaa gctgttttcc tgcttttcat gcaataatgc
    41521 tgttgaaggc ccgtggccgg gataaaccat tgtgtcgtca ggcagagtaa aaagcttttc
    41581 tcttattgag ctaaccagtt cagtcatgga tcctccgtac aaatctgtcc tcccaacaga
    41641 gtttttaaac aaggtatccc ctgaaaacaa tactttgtca caataaatgc ttatacctcc
    41701 ccgggagtgt cccggcgtat gtattatatt gagcttcgta tcgccaagct caagtacttc
    41761 cccgtcttca aggtacttat ccgctgtcat ttcaatcgct gctccgccca acatgcccga
    41821 aaaattcatt gttttgctct tcagcatttc actgtcttct ctatgtatgc aaactaaagc
    41881 gcgggaatac tccttcagtt ccttcagggc accaatatgg tctccgtggg catgggtgat
    41941 tattatatat tttatattta atccgttggc ttcaatatag ctttttattg cgggaaaatc
    42001 ccctccggga tcaattactg ccgtttcctt tgttttctcg tcataaacaa tatagcagtt
    42061 ggtttgatat attcctaaag ccagactttt aaatttcatc atataccagc acgtctcctt
    42121 ctttatatta tcttctcact atccacaatt actgtgacag gtccgtcatt ttcaatatta
    42181 accttcatat atgcctgaaa ggttcctgtt tcagttttca ctcccagttc cctgcacttc
    42241 ttgacaaact cattgtaaag ctcttcagcc ttttcgggcc ttgctgcact cataaaattc
    42301 ggccttcttc ccttcctgca gtctccatac aatgtaaatt gtgaaatcac aagcaactca
    42361 cccttaatat ccagcagaga taagttcatc ttctcattat catcctcaaa tatcctcaaa
    42421 ttcactgtct tgtcaaccat gtacagcaca tccgcctcgg tatccttctc gtttactccc
    42481 agtaaaacga caaagccctt gcctatgctt cctactattt tgtcatcaac agcaacactt
    42541 ccgctgttaa ccctttgtat aaccgcccgc attatttacc ctcctgtaac tttgcatcat
    42601 tgctttgctc tgaagacatc cactactcct tcgattcttc tgaactttct tataagctta
    42661 ttcagctgtt caatatcggt aatatccaaa atgaaattga tgattgccat cttatccttt
    42721 gttattctgc aatgaaagga actgatattt atcttgcttt cattaataat cgtagttacc
    42781 tcggcaagca atcccttcct gtccattgcc cttatttcca catcagcgct gtatgattcc
    42841 tttgtctggc tgctccattc cacctctatg aatctttctt cctcgttcaa aaggccggct
    42901 acattggggc agtccttctg atgcaccgac actcctcttc ccttggtaat atacccgaca
    42961 atttcgtcac cgggcacagg gttgcagcac tttgaaaacc ttatcaatat gttctccacg
    43021 ccttttacct tgactcctgt gctgttatac cttttcttcc tttcctgagc cttctcagcc
    43081 tggcgttcga tgctatcctc agtaatctct tcaagcttct gggtttttct gaattcttcc
    43141 ttcagttttg ctattatctg attgggtgtc aaacctccat agccaaggga tgcgtacata
    43201 tcctccaaac tgtggaggct gaatttctta tatatggcct caacccattc gggcctcatc
    43261 aactgaacgg tggtatatcc gtttctcctt atctccttct ccagcagttc ttttcccttt
    43321 tgaatattct cttctctttt ttccttctta aaccattgtc ttatcttatt ctttgcctgt
    43381 gagcttttga ctattttcag ccagtccctg cccggtccgt tggcaacggc agaagtaatt
    43441 acttctacga tatccccgtt ctgaagctta taatccaatg gaactattct tccgtttatc
    43501 tttgctccta tgcacctgtt gcctatttgg ctgtgtattt tatatgcaaa atctatcgga
    43561 gtagattcca caggcagatc tacaacatca ccctttggtg tgaatacata tacttcatcg
    43621 gtaaacaagt ccaccttcag ggtctccatg aactctctcg tgtccttgat ttcgttctgc
    43681 cattcaagca tctgtctcag ccatttcagc ttttcgtcaa actcggactg ggaggtcttg
    43741 ccttctttat atttccaatg agccgcaatg cctatttcag atgtcctgtg catttcccat
    43801 gtccttattt gtatttcaaa gggctgtccc ccaaaaccca gcaatgtcgt atgaagggat
    43861 tggtacatat ttggcttcgg catcgcaaca taatccttaa accttcccgg tatgggcttc
    43921 cataaggtat gcactatgcc cagcactcca taacaatctt ttaccgtgtc cactattatt
    43981 cttactgccg taaggtcgaa tatctgctca aaggtcttgt tctgatagta cattttcctg
    44041 tatatgctgt aaaagctttt cggccttccc tcaatatgag cctctattcc tacttcctcc
    44101 agctttccct taaggacctc cataacctga ttgatttcat cctcgcgttc cttcctcttc
    44161 ttggcaacct tgtccaccaa ttcgtaatac ccttttggtt caaggtatct taacgccagg
    44221 tcttccatct cccactttac cttagatata ccgagcctat gagcaatagg agcatatatt
    44281 tccattgtct ccctggcctt ttccttctgc ttttcaggag acaggaatct aagagtacgc
    44341 atattgtgca gcctgtcggc aagctttatc ataatcaccc ttatatcctt agccatagct
    44401 atgaacatct ttctgaggct ttccgcctgt tgctcctctt ttgtcttgta ttcaagcttt
    44461 cccaatttgg tgaccccatc tacaagcatt gcaatctctt cgccgaaaag gttcttgcaa
    44521 tcctcatagg tgcattttgt gtcttcaata acatcatgaa gaaggcttgc agcaatagtg
    44581 atgttgtcca tattaagctc ggccagtatt ttcgccactt ccagggggtg aaaaatatag
    44641 tcctccccgg atactctctt ttgtccgtca tgagcccggg cagcataatt ataagccctt
    44701 tcgacaatct ctgcgtcacc ggtcgggtta tactttttaa ttattttcaa taaatcttct
    44761 aacatagcaa caccaacttt taaacgttaa atggactgcg tatacaagta ctactaaaaa
    44821 agctggttgc gccagctttc atatatttac atttatttta gtataaattc cttcaattat
    44881 caaatataaa taataactct ccaatattaa tattttataa tcgaacttac atcataccct
    44941 tttaaattct ctcttccttt caagaattcc aattctataa gaaatgcaat gtgcactact
    45001 tctcctccaa gctgttccac caatctgacc gcagacaata cagtacctcc ggttgcaagc
    45061 aggtcatcca ccacagccac tctcgtacct ttttttattg catctttgtg aatctccagg
    45121 gcatccattc catattccag ctcatactta tagcaggctg tctcagcagg aagcttgccc
    45181 ggtttcctta tgggaacaaa accaacccca agctcatatg ctgcaggtgc tccgaaaatg
    45241 aaccccctgg cttccggtcc tacgataata tcgaaatcat aattacttaa attatctgca
    45301 atggatttca cagcgtattt gaaagcatct ccctctttaa gaagggttgt cacatccttg
    45361 aagcttatgc cttccttcgg gaaattttca attgttctta tatattttat taaatccata
    45421 aatactaccc ccatttatta atttatgagt tttatatcct ttatgttcag ctggacactt
    45481 tctgttccgt tccatatgtt attctcaata ctacatatta tatcaatttt ttcgccgatt
    45541 tgtaatattt tttgcatatt tcccaaatta taacctatgc agctgatttc cttttcgccg
    45601 tcaaaaacct ttagagagag gtgtctgcta tcattcccaa ctcctttgaa agatgtgacc
    45661 gaaagctccc tgaacacata aagagggact gtattgcctg tgccaaaggg ttccatcagc
    45721 ttcagcagtt ttgcagtatc aagggtaacg tctttcggct caagcttata atcaacaaat
    45781 atttccggta tgagcttatc ttcgcctaaa gtattttcaa cttcaaaatt catcctctcc
    45841 ctgagttcgt ctattttttc ggttcgtata gtaaagcctg cagccatttc atgtccgcca
    45901 tacctttcaa aaagatatcc gtgtctggac attgcctcaa aaatattgaa accgggtatg
    45961 cttcttgctg accctttgca gaactccccg tcacaaacca taataaacgc agggagataa
    46021 aatttatctg ccagcctcga tgccacaatt ccaataacac ctatatgcca atcctccctg
    46081 cacagaacca ataccttatc cctatccaaa tcaacgcttt cacctatcat agcttctgca
    46141 cagttcaata ttccattttc tatcgattgg cgcatcttat tgtctgaatc cagcttggca
    46201 gctgtttcca ttgccttttc cgcattatcg gtcagcaata gctcaacaca ttcgccggca
    46261 tcgtcgattc tgcccgctgc atttattctt ggagcaagca tgaaggccac attatatgaa
    46321 ttcagctctt tgccctttaa tccgcatacc gcaagaagcg cttttatgcc ttgactgtcg
    46381 gtattttgga tacttacaag gccgtttttc acaattatac ggttttctcc caacaatgga
    46441 actacgtctg ctatcgttcc gattgcagca atatccaaaa gctcttctgc aaaaccgggg
    46501 ctgcctgccc tattaagcag tgcgacggca agcttgaacg taaccccgac tccggccaga
    46561 tccttgaagg gatacccgca gcccggctga tgcggattga tgatgcttaa agcatccggc
    46621 agctcttccc cgcactcgtg gtgatccgtt ataatgatat ctattcccag ttcctttgca
    46681 taatcaactt cctcacagga ctttataccg cagtctacgg taactatcag gcttaccccc
    46741 atctccttaa ttctgtcaat tgagttgagg ttcattccgt agccctcttc caatctgttc
    46801 ggtatatagt ataccccatc agccccaagc ttcctcaatg tctttatcat tacagcggtt
    46861 gaggtaattc cgtctacatc gtagtcaccg taaatgcata ttttctcatt gcttctgact
    46921 gccttaaata tcctgtctgc tgcctgctcc attcccttca aaaggtacgg atcatgaagt
    46981 gagcaaaggc tggtgttaaa aaattccctt gctttcggaa cagtatcgat acctctgttt
    47041 atcatagctc tgataattat ctccggtaat tgaaactcct caatcatcgg attaaggcta
    47101 tccatgcttc tgctgtaagc tgtccatctt ttgttcaata ttcatcacat gccttactga
    47161 atttctggtt taaggttatg aaatatccat tttatctata tcttctattg tctgaggctt
    47221 ttcctgcata acaggttcct ttgccggaag cttttccgac tcaatccttt tgagttcttc
    47281 cttaatcttt ttgttttctc ctgtcagctc cctaagcttg aatttgtatt ttattcttct
    47341 tataatatcg aaaggaagca taatcaatac cccaatcaat gcagaacaaa gaattaccac
    47401 agcttgtgac aggcttacct tataccagag aaaatttatg acaactactc ctgcgttttg
    47461 taccgcaaaa aatgcaataa taacagcaaa aaccaatgaa attacaatag taaattgcat
    47521 ataaaaccct cccgccatta ttgattaaat atatgtaagt caccaagctt ccatgcataa
    47581 agtatacctt cactgctgca aaaacttgat actttaagtc taatatatag cttttcggcc
    47641 gatacttcaa ctgttttcta acagtcctgc tgcacaatac aagaatgatg cgtcgcatct
    47701 gtagtttgca taagcctccg caagcaaggg gacatgggga cgtttcccct gtcccgcttt
    47761 cagtgggaca cgcgaaacgt ccccgtgtcc ccgcgtcccc atgtccccgt cgcattattc
    47821 ttctaatgcg aaaagggata tcgctggcta cttatcaagt agtcttgcga caccccttta
    47881 ataacctgta tcagcgggct ctgcttactt taaatctcct tctcagtatt acccataacg
    47941 ggcttgcaat gaatatggtg gaatagcatc cgcttactat acctataatc atcggaaatg
    48001 cgaaatccct tattgcaggt acacccaaga aatacaaggc aaatattgta attaaagtag
    48061 tcagtgaagt atttattgaa cgggcaaggg tctgagtgac cgatacgttg accagattat
    48121 cgtaatcaat cttcttgctg tgttcgttct tcaaattctc tctgatttta tcaaagatta
    48181 ctatagtatc atttattgag tagcctatta ttgtcaataa cgcagcgata aatgtactgt
    48241 tgaccggtat ctggaaaacg gcatacgctg caaccatcat tgcagtatcg tgtattaatg
    48301 ccgacaaaga agccagtcca aacctccact caaacctgaa ggatacataa atcattattc
    48361 ctgcgaatgc aagcattact gcaataagtg actgcatagc caattctttt cctacggtag
    48421 gccctattgt ttcaaaagaa atattatttg attctattga atacttatct gaaaaggctt
    48481 ttattatctc tgcccttttt tcattatcaa agtttatctt ggttcttact atgacctgat
    48541 ccttgttttg tcctgcatga gttattgttg cttccggatc gtatttgtcc ataatttctc
    48601 ttatttcgga tacatcaaag gttttcccga tggtagcatg aatagagctt ccccctacaa
    48661 aatccatacc cagattcaaa ccgccgttaa tgatcaaaaa tatcaatcct acagccagca
    48721 gggcaagtga tattccaaac catatttttc tgggtcccat aaagtcaatg gtcttcatat
    48781 tacttcactc ccctttcacc aaaatatctc atgctcttta atatgcctcc gctggtcagc
    48841 agcttcagca tgatctttgt aatagtcaat gctgtgaaca tactgacagc cacgcccaca
    48901 atcagggtaa ctgcaaagcc tcttatgggt ccggatccaa agtagaacag tataacacag
    48961 gcaattattg ttgtcaggtt ggagtcaaat atcgtaagga atgctctcct gaaaccgctt
    49021 tctatggctg ccctcaaagt ttttcccaaa ttcagctcgt cctttattct ttcatatatt
    49081 actacatttg catcaactgc catacccact gaaagaacca aacccgctat acccggcaaa
    49141 gtaagtgtcg ctttcattgc cgccagtgca aataatgtaa gagtcaaata gagcactaaa
    49201 gtaatatctg caacaaggcc gggtattcta taatagaata tcataaaaat gaaaaccagt
    49261 aatatcccta tctgccctgc gttaacactc tgttcaaaag aattcgcccc caattgaggc
    49321 cccactgctg ttactgacag ggtctccagc ggtacgggaa gcgcccctgc cctaataagc
    49381 gttgcaaggt ctcctgcctc ttccatggtg gctgagcctg taattatacc ttttccgtca
    49441 ggtattacac tttctacttt tggaagtgat atcggattct tatcgagata tatcccaatt
    49501 attttattaa tgtttctttc agtagcctcc ttaaacttct ttgctccttc cggtgacagt
    49561 tcaaaggcta ctaccggagt ctggcctaat ccgtctttgc ttggtgcgaa ctctgcctga
    49621 gctttcttta cgtcattgcc cgtcagtatt accgttcctg tttcttcttc gataaattcc
    49681 agctgagcag tctgtccgat caagtccaaa gccctctgcg gatcctgtat ttcaggaagc
    49741 tgcacctcta tcctgtctgc tcctactctt gtaataatag gttctgttac tccgagagta
    49801 tctatacgtt ctctaatagt tgcaactgct cttgccatct tctcatcatt taccggatca
    49861 tcgggtttat cctttgcttc cagcagaaca gtcactccgc ctcttaaatc aagacccaat
    49921 ttgataaggt ctttagccgg tacaatttca tattttccta tattaagccc aaaaaacgct
    49981 atatatgcaa aaaatgaaat taacaaaact attgcaagaa acaatattag gctctttgac
    50041 ttcatttgct tattccccct tatttgattt cacaatggtt attatattac ttttacaatt
    50101 tttagtcaat gaagagaagt attcccattg cgaacatcag gcttccatat gtattatttg
    50161 ctgcttttct tattttatcc aatctgcaaa ttactgctta aagctttact tattctattg
    50221 acagctttga atactctttt gaaagaatta tatattcttc tgcagagtgg cttatacctt
    50281 caatttccgc aggagccagt tcccttacaa cctttgcagg actccccata acaagaaccc
    50341 ctggcggaat tttcttgcct gaagtaatca atgaattggc tcctactatt acattgtctc
    50401 caataactgc accgtcaatt ataatggctc ccattcctat caggcaattg ttccctaccg
    50461 tgcatccatg aatgattgca ttgtgcccta cagtgacatt ttcaccgata atggttttat
    50521 aatcatttgc acagtgaaca acgcacccat cctgaatatt agtgcctctt ccaatgatta
    50581 tttcatttac gtccgcccta accaccgtat tataccaaat gctgcagttt tcgcctatga
    50641 tgcatctgcc tataatcgtg ctgttttctg ttataaatgt gtttttccct atgtttggtt
    50701 tataatgctt gtacgatatt atcatatgcc ctccatcaca catgttttaa gcagtaacat
    50761 aaagggtctg ctctaaagta tccccttata ttactgccta tgtaccatag ctatatatga
    50821 tattccatta aatcgtattc ttatgtgaag ttcccgtcct aaccctgctg ttctgaatat
    50881 tttcacattt ctgatttgct actgtacagg aagtcttgca cgctgactgg cacgaattct
    50941 gacattcacc gcagccgccc ttttgtacgc tgtcatataa tgatcttttg tttatcgttt
    51001 ttacatgctt cattctgttt cctcctccat gttttgtcac tgttatgatt atagcatatt
    51061 agttttttat gggaaagaaa aatctgcctt cgtatcaaca aaaaaccggc tcaattcagg
    51121 tttatcccta ttattcctcc tatcagccca acagaactcc ccataagcat cttcgccgca
    51181 aaatacttgt caaggctcag actctgtccg aagcacatac ccaagggtac cagcagcagt
    51241 gtaaatacca gtcccaatac tattccaaaa aaccagccct tcttcagaat cttcttgcct
    51301 ccgtaaatac ttgaaattgt tatgcttaca atcattatta tctgtattgt catgctcatt
    51361 gatgaatcac tcatacttgt aaacgacagc accagcgcat acagcattat cagtatgaaa
    51421 gacacaccaa aacatattcc gagtgatttt aagaataccc catatatatt aattcctttt
    51481 tcttctgcag gcggcatctt agtatagctc ttaaactttc tcatgaaaaa taacccccta
    51541 tagtattttt acaaggtttg ttttctataa atttattcgc tataagggac atttattctc
    51601 atatgacgaa aattcagggg aatgaagaaa agtaacttgg atccaagtta cttttcttca
    51661 ttgttctcta ttacctttcc cactgcccat cttgcaattt tgagctttac tttatcagcc
    51721 ccaacctcta tggtaagtac atcttccttt gcattaacca cttttccgta aaatccccct
    51781 atggttacaa tttccatacc ctctttaaca ctgtttcgca tttcctgcgt tgctttctcc
    51841 ttcttcttct gtggtcttat cagaaaaaag taaaaagcag ctataacaat tgcataaggt
    51901 aatatccatc caaactgctg catactttac attcctcctt tcatatctta ttccaccggc
    51961 ataattattc cgaaatagct cctaaatatg aatgcaagct taaacaccag tggctggtat
    52021 tattctctga attatactat tcaatataga agtcaaaaat ccttcccgct atacaactta
    52081 tactatatta tttttcatat ccatactttt caaagaagtc attcctaagc tcagtaagct
    52141 tatcctccat tatggctttt cttatctcag acatcagatt ctgaaggaac ctgaggttgt
    52201 gaacggtggt aagccttcct cccagtattt cacctgcctt aataagatgt cttatatagg
    52261 ctcttgagaa gtttctgcag gcataacagt cacattcctc gtccaagggc ctgaaatcct
    52321 cactgtattc tgcatttctg acaaccagct ttcctcttga ggtcatcaca gtaccgtttc
    52381 ttgcaattct tgtaggcagc acgcagtcaa acatatctat tcctcttatt gagccttcta
    52441 tcagacaatc agggcttccc acccccataa gatatctggg tttatcggag ggaagctccg
    52501 gcactgtata ttccagcacc tcattcatta attccttcgg ctctccgacg ctcagacctc
    52561 ctattgcata ccccggcaaa tccatttcta cagttctttt ggcactttca atcctcaaat
    52621 ccttatagac acttccttgc actatcccga aaagagattg cttctcggtg ttattatgtg
    52681 cttccttgca ccttttcaac catcttgcag ttctttccat ggactttttc gcataatcat
    52741 gctcacaggg ataaggtgca cattcgtcaa aggccattat aatatcagcc ccaagcgaat
    52801 tctcgatttc catcacatat tcgggagtga aaaaatgcct ggacccatcc aggtgtgatt
    52861 tgaattccac accttcttcc gatatcttcc tcagttcatt gaggctgaat acctgaaatc
    52921 cgccgctgtc ggtaagaata gccctgtccc aattcatgaa cccatgaagt ccccctgcct
    52981 tctctaccag cctatgcccc ggccttatgt acaaatgata tgtattgctg agtattatct
    53041 gcgctccggt ttccttcagt tcaagaggag acatagcctt taccgtagct tgggtgccta
    53101 ccggcatgaa taccggagta tcaatgactc catggggggt atgcagcttc cccagccttg
    53161 ctcccgttct tttttcttcc tttataagct cgtattttat agtcaactaa gtatacctcc
    53221 attaaaacag cagaatatat ttatcatgat tcattgtatt tcgctgccca taccctgtgt
    53281 atgactccat cgttatagct cagcaccttt aactcagcat ctgcaatccg gtaatatgag
    53341 ccttcatgga tatccctgca ggattgcttg gataaacggc ttattaaacc gcctacggta
    53401 tcggcgagat cttcaggtat ttttacacct attgtgttat ttatatcaga tatactggca
    53461 gaagccggga aaatattgat agcttcgctg tcccttaaat tgcttataaa cagatacttc
    53521 tttattgcaa aaatactggc tatagagact attccgagaa aaaattccag catatgtgca
    53581 ttgcttatca gcatttttct tgccacagca taaagtaaaa cctctattac actgccgggt
    53641 gtgtgcctga caagcatggc caccatctca acccctacta cgaggagaag tgtatgcccc
    53701 agaaactttt gaaacacctc ataagtttgc aaagtactgg tctgaaatat caacacaata
    53761 tactttgcca gatctatcat accgataact atagctaata taataactat tgccaatatg
    53821 ttctccaaat acagactcgc attaataatg ctgtgtttaa ttcgagtcat tttcattgaa
    53881 acacctccca aaattaaata ataagcatcg catctccaaa gctgaaaaat ctgtagtgtt
    53941 ccttcaccgc ttctttatat gccctcatta tgttatctct gcctgccaaa gcacttacaa
    54001 gcataagcaa agtagattca ggcagatgaa aattagttat aaggccatca gttattttaa
    54061 actgataccc gggataaatg aaaatgtccg tccagccttt gtcttccttc aggcttcctt
    54121 ttctctgcat tatcgattcc agtgtccttg cagaggtagt gccagtacat attattctgc
    54181 cgccgttttt ccttgtattg tttataatct cggcactttc agcagtaagc tcatagtatt
    54241 ccgaatgcat tttgtgctcc tcaatattgc tgacctttac cggcctgaag gtaccaatgc
    54301 ccacgtgaag cgtcaaataa gctatattca cacccgcctc tgccagctga tgcatcagct
    54361 catttgtaaa atgcagcccg gcagttggtg ctgcagctga gccttcaatc tttgaataca
    54421 ccgtctgata acgttccctg tctgtaagtt ctttcttaat ataagggggt aaagggacct
    54481 ttcccaagcg gtcaagcact tcctcgaaga tgccttcgta gttaaattta accaggcgat
    54541 tgcctccttc aagtatttca attatctctc cggaaagctc tccgtctccg aatataaatt
    54601 ccgcacctat ctttgccctt ttccccggtc ttaccatcac ttcccagaca tatttatcct
    54661 tccgattaag caaaaggaat tccatatttc ccgtgctgcc cctcttaaca ccaaaaagcc
    54721 ttgcgggtat tactcttgta ttgttcatca ccaggcagtc tccctcattc aggtattcca
    54781 aaatatctct gaaactgcgg tgcacaattt caccggtgtt tttatcaaga accagaagcc
    54841 tggacatatc cctttcgggg atcggctcct gtgcgatcaa ttcctcagga aggtcatagt
    54901 gaaaatcctt aagttccata ccgtgctctc tttctattgt acatttgcgt tctgatagta
    54961 gtgttccaat attttctgat agttataacc tgcttcagcc ataccttttg caccgtattg
    55021 gctcattccc agaccatgcc cgaaaccctt cccgtcaaag gtatatttgt cgggaatcac
    55081 attataggct ttagtatcgt tcatcccttt aacgcttacc ttgttcccct ttcctttaac
    55141 aactgctttt cccgatgcag aaacaacata catatttgaa gttcttccct tttcggcaga
    55201 atttaaaatg gaacctctta caaagacatc cgcatccgtt ttcaaatcgt accatatgct
    55261 cttaagcagc ctcgtaccca atatgctcct tatcttttcc ttctcgaaaa ctgttgtatc
    55321 cttggagccc tttacctcca gcttggtaac tctcccgaac ttactgcact ccaatattct
    55381 gacatctctt atttcaccca aatctacacc ggattgggca agcttttctt tcaattcagc
    55441 cctgttcagt tcaaccgtcc agttgtcatg ggggcttccg aggctgaatt tatcttccac
    55501 ccctctgata taatcagttt ttgcacccca aacattctca ctgtcttcag tgtgccctcc
    55561 gctgcttgag tggtaaaaag cggatattag cacaccatta tatgtaatta ctttgccctt
    55621 ggtggcatta acagcttctg tggaattact gttttcctgc gccaatccgt tatatgcctg
    55681 gcagtgctca gtactgcaca ggtcaaagcc atgagcatta tgccttccca tagtcaccag
    55741 cgcaaagttc cttgccgcca ccgcctgtgc cttcaaggct tctatatgcc aggaggccgg
    55801 catctcagaa ggaacaacac tatacaaata ttgatcaagg gaaagctcat ttataactgt
    55861 gatatcactt tcttcaaaac tttgcattac tatgctccct ctgtatttct ttcctttata
    55921 ctgcagtgaa cttacttttc cattgacctc cttgggtgta actttaacat ttccctcact
    55981 gttcaataaa agcataagct ttccggtagt attgtcaata atctgtattc ttttcttgtc
    56041 aggatataca acagaatatt ttaaatcact tgcttccttc ttcataatct ttatctgttc
    56101 aaggcattcg ctttcatcaa gatacagctg tgaccacacc ctccatccct cttcatatgc
    56161 aagaaatacg gactgggtaa tggatgatac ctgttttaag acttgcaggg cggcttctgc
    56221 attagcatac acccccccta tttgtatatg atatgggccg actacttcac cctcgtattt
    56281 ggctgcccgg acatagttta tttcacattc cttgccgcct gcaatattat agtattcatc
    56341 cctgcggacc ttcacaacct taggattttt cacttcaagc aaattcttgt atgagccctt
    56401 ctcaagaata agaatattca tccctgtctc acttgcaaga gtaaaaatat ttgcctggga
    56461 ttgtccaaag ctcaggccga ttctgattgt ctcaggaact gcaatatcgg catgcaaaat
    56521 cataaacata actgaagtaa ttaccaaata gagtactgca aatttttttg acttcataag
    56581 cacaaactcc ttttatcacg cattccttgc actatctgcg aataaaaccg aatactatcg
    56641 tcattataat acttgcaaga atacaggtta ctatcggaaa ataaaatgta aaatttccct
    56701 tttttatata aatatctccc ggaagcctcc caaatcccag tttgccgcca aagtacagca
    56761 gtgtcccgaa gataaccatt ccgattccaa gtactattat tattttggca aaatattgaa
    56821 agttcatttt cgaccgacct ttctcccggg catgtatctg tccctttcgc tgtaaataca
    56881 gccgcagtat ggctgcctgt acaagcccag ttccctggat ttttcaacac cctccttgaa
    56941 gcctcccctg aaatccttat ataggaattc cactccgtat ttcttccctg cttcctctcc
    57001 tatcctcctg ataaggtcat gcttctggaa agggcttacc agaagtgtag tggtaaaagc
    57061 atcaaaacag cctgattttg caacagaagc ggctctctcc aacctttcgg cataacacat
    57121 aatgcatctc tgcccttcac ggaaagctgc attcctgaga aattcatcca aattatattc
    57181 atcaatggta atcagcttgt aacctgattt gtccgcaaaa tcccgggctg tgtccagcct
    57241 tttcttatac tccgtataag gatgtatatt ggggttatag aataaccctg ttatttcatg
    57301 ctcccggctc agaacctgat ggggataaac ggcacatggg ccgcagcaca tatgcagcag
    57361 cagcttcaat actctcactc ctgattaatc tttttttatt ccaaaatgcc tgtatgcgga
    57421 atttgttgca actcttcccc ttggtgtcct gttaataaat ccgatttgaa gaagatacgg
    57481 ctcgtaaaca tcctcaatag taccgctgtc ttctcctatg gatgccgcaa gagtatccaa
    57541 acctaccggc ccgccgtcaa atttattgat aatggtaagc atcatctttt tatcaatgtc
    57601 gtcaagcccc atgtcgtcta tttcaagaag cttcaatgcc ctgtcggctg tttccttgtc
    57661 aatgactccg gaagctttga cctgggcaaa atcccttacc ctcttcagca atctgttggc
    57721 aactctcgga gtacctctgg atcttcttgc tatctcaaag gctccggctt catccgtatc
    57781 tatcttcagt atccttgcag accgcttgat tatcttgaaa agctcttcat cagtataata
    57841 ttccagcctg cagattactc caaacctgtc tctcagaggt gcagtaacca taccggctct
    57901 tgtggtggca ccgatcatag taaatctagg caggtcaagc ctgattgagc gggcactggg
    57961 cccctttcct attattatat ccaaagcata atcttccatg gcaggataca gtatctcttc
    58021 cacacttctg ttcagcctgt gtatttcatc gataaaaagt acatcattat tatcaagatt
    58081 ggtaagaatt gccgccaggt ctccgggctt ctctatggca ggcccggaag tgattcttat
    58141 gcttacgccc atttcattgg aaataatatt ggcaagagtt gttttcccaa gtcccggagg
    58201 cccgtaaagc aggacatggt ccaaggtttc cttcctctta ttcgctgctt gtatgaaaat
    58261 gctgattttt tccttggcaa cggactgtcc tatgtattcc tcaagggatt tgggcctcag
    58321 gctggtatca atctctatat catcttctat tatctttggg gttatcaatc tgtcttccat
    58381 tgtatttcct cacttttatc agttcaatat tacttcatgg tcccaagctg cctcaatgct
    58441 tccttaatca agatatcgat aggtttgttt gtatccttta gcttcatcac cgcatttgcc
    58501 gctatagaat aattatatcc taaagcggtc aaagcttcaa ttacctggga tatctcgtcg
    58561 ctcccgccga aagccccgat tgctcccact gcgctttccg tatctatttt gtctttcagt
    58621 tcaagtatta tcctttctga aatcttcttt ccgataccct gcgcttttga aagctttgct
    58681 acgtccctgc tcaatatggc tgctgccagc tccgatgcct tatattgatt caatagggaa
    58741 gctgcggcct ttggccctac tccggatacc gatataagca gtttgaacat tccaagctct
    58801 tcccttgtag caaatccata tagtatcagg tcatcctcct tgactatcaa gtgtgtaaaa
    58861 atctttgaaa tctcacccag cttaaggctt ttcattgaat tctgcgaagt aaacaccctg
    58921 tagcccaggc ctgaaatgtc aattacggca taatcctctc cgatatagtc cactcttccg
    58981 cttaaatact catacattgt acttcacccc tacactctga attgttccga gaattttgac
    59041 gaattgccgt ggcaaattgc aacagccaaa gcatctgcga catcatcagg cttcggtatc
    59101 tcaccaagat tcaatattac cttcaccatc tgctgcattt ggttcttgtc agcccttcca
    59161 taacctacta cagcctgttt gacctgaaga ggggtatact catatacctg cacgccggat
    59221 tttgagccgg ccaggacagc tactcccctt gcatgtccga ctgtgagagc tgtcttgata
    59281 tttttgttga aaaacagctc ttcaatggca aaggcatccg ggttatgcct ttcaataata
    59341 tcaacaagcc tgtcataaat tatggtaagc cttgcagaca tatttatgcc tgaatctgtg
    59401 gtaatcgctc catagtcaat aaccgtgaat ctgtttgctt catattttac aagtccataa
    59461 cccagtattg caatacccgg gtctacgcca agtatcaaca atagatatac accaccaaac
    59521 atatattcgc tatatttact ataacttttt tggggaagaa atgcaacagg gcagcaggat
    59581 tgctgccctc aaattgctga caaagctcaa aataggaaca gtaatgtata gtcagagcat
    59641 aattatacag aaattgttaa tggctcttat gaggctcgac ttagaaacaa ccgtatgcga
    59701 ggcatggagg ccgagcaagc tgtctcggtc acggatgacc tttgacagcg ttaggttttt
    59761 cttagttgag ccgaattaga gccatcaatt tttttgagac acggggacgt ttcgcgtgtc
    59821 tcacttcagc gagacaggag aaacgtcccc gtgtctcatt tgctgttctt atgttgaagg
    59881 tttttcatca gcctcagggc aatagaattg ctgccttgaa ttaacggaca taagaagaat
    59941 gggttgcgtt atttaattgc tgaagccctc cagggtctgc tccttctgct cctctgtatc
    60001 taccactatc ccccccgcaa gggcctgctg atcctcagga gtaagcggag ctgcggaaat
    60061 gtctgttttt tcctgcaggg acttatgccc gttctcatca tatacataaa tcgcaatata
    60121 cccgccgtcc actcctatta tataatggcc ggggcagtaa gtatcaatat tcctgctgaa
    60181 ctcaactaca ggaggtgaat aacctgaaag gtgccaatgg ctgtattttt ctttcagctg
    60241 ctgctcattc atatttatct cgtcgcttcc tgcctgaaca gctttttcta cggtatgcct
    60301 gcaggaactg tagtatgtct tgaaaatcaa ctgagtattc ggtgtgggaa aatctatctc
    60361 taaaatattc gctgtctcct ctgtattgct ttcggcatta acctcttcat agggctttct
    60421 cactgttgtc ggattaatca ggctgtcagg tatctgaagg ttggcagatg gctgattatc
    60481 aggattatct aaagcttcag ggatatcagc attgaaaaaa tacccgaccg caaaggcggc
    60541 ggaaatcagc agtagaaagg ccgcagccag cagatacctt cttttgcgtc tgatgcttct
    60601 atcttcaaac ataattatcc ctccatattg ttctggaggg tattatttcc aaaatatata
    60661 tttttatacc tatttataga atattcttct tttacctgcc tgttagctat atcattttca
    60721 aacttgtcat gttgtcatag tatacggtat acgtatcaga tttgtgttat ttcagatact
    60781 attcgttatt aataaatgaa aatgagggtt gatactatga caggattggg cttcgcgtgt
    60841 atctcaaaga gcttgaagga aaacggcaga ttcaggacaa tgagcgtaaa aacctataat
    60901 agcctgaaac ctgatgaaag gattcgaagg cttagatcca tatcagtgga taacctgtac
    60961 aacacctgca ggattatact ttggtgcatt gaaaacagta taagcctgta tcggttaagc
    61021 tcgggcctta tacccctggc aggctgcgta ccggaatgga aatggtggga agaccgggat
    61081 ataaggaaca tgtgtgaaaa gataaaaaag attgttgatg aaggcaaaat aagaatcagc
    61141 atgcatcccg accaattctg cgtgcttaac tcagacaagg ctgaggtagt aaagggaagc
    61201 attgcaattc ttgaacacca taacagactg tcaaatatgg tgggaaacag gattctagta
    61261 attcacgcag gcagcggcac cggaggcaaa agcaaagcaa ttgaaaggtt catcaagaat
    61321 tttaacatgc tggatgaaga tattaagaaa aaaattgcaa tagaaaacga tgacaaggtt
    61381 ttcaatgcgg aagacattct gcacctgtgt gagcatctgg gagtaccgat ggtgctggat
    61441 atccatcatc ataactgcaa tcatgaaaaa gacaacctgc cccagctgat ggatcgtata
    61501 aaaagcacct ggaagggaaa aaggccaaag gtgcacctta gcagcggaaa aacctgccct
    61561 accgacaggc accatgcaga ttatatagat tttggcgatt acaggcaggc agcagaactc
    61621 gttaaagatg aatttgatat aatgatggaa tgcaaggaaa aagacctggc agtacttaag
    61681 attctcacat attgcatctg atttcaagat atactgtaca atcatccttg aatataaatt
    61741 tattgttaaa gcaaaatgtt taaaacgatg aaggtattat aggaggatgt ttgacgtgtt
    61801 cataaatgga aaaatcccgc accttttgca gcagactgtg ctcatgactg cttttacatt
    61861 attgaagttc actgccttgt accactttgc cggaaagcct caggatatgc ttcctgcagc
    61921 tttaaggaca tatacgctcc tgtttgccat atatttattg accaacctgg tcataggaag
    61981 aaaaagcttc ttaatcagaa tagcctttca tgaatttctg gctctcattg tattggcaga
    62041 tacgctttat tacaaacaat tcaatatact gccggaagtt gcggatctgc agtttctgaa
    62101 agtcctgccg acaatatggg acagtgtagg ctttcttttt tcagggatac accttctgct
    62161 tttcgccgat gccctgattt tgatgactca ccaatatgcc ctgaacagac aggaaaaggc
    62221 ttcagcccct gggatactgc cctctgttat cgcagctgcg cttttgcttg caattatatt
    62281 tacagacttc tctttcagcg atataaaaag cagcggccag gcctatgaaa aattcggcct
    62341 gctgcattat catatttcac aaattccggg cgtattgcag tccaacagca acagcagcag
    62401 ccggagtatc ggcaaggctc agattccggc agaggttgag gaagctgcta aagagcccag
    62461 gtgctttgga attgcaaagg gcagaaacgt aattgtaata caggtggagt cccttcagga
    62521 ttttgtcata aatatgcatt atgaaggtca ggagcttacg ccgaatctga acaagctgct
    62581 tctgaaagat acaatatact tcaaccgttt ctaccagcag ctggggaaag gcaatacttc
    62641 ggatgccgaa tttgtcaccc agaattccct atatccttcg attgatatac ctgcatacag
    62701 aaaatatgag aataacagat ttatggggct ccctatgatt atgagagaaa caggctacgg
    62761 cacctacgcc tttcatgcat ataagccgga cttctggaac aggcagaata tgtatcccat
    62821 gcttggtttt gaccgtttta taaacatgaa cgactttaac cttaacgaga catttggtat
    62881 gggcttaagc gacaaggaat tttttgagca atcggccgca tatatcagta ctgccaatca
    62941 gcctttttac tctttccttg ttactttatc cagccatcac cctttcggcc taccgcaaaa
    63001 atacaagaaa atacagctgc agccggcaca taaggatact ctgttcgggc gctatattca
    63061 ggttattaac tacactgatg aggctatcgg gctttttctc gaaaaactga aagaatacgg
    63121 cttatatgac aactcaataa tagcattata tggagaccat cgtagcattc ccaacggcgt
    63181 ccctgaaaac gacaagctga tgtcggagct cttggaccat gaatacactt ttgatgagtc
    63241 tctcaatgtg ccgcttataa tacatattcc gggaaccaat ataatagaaa ccaatacaat
    63301 agtcggaggt caaatggact ttctgcccac tatgctgaat ctgctgggaa taaaagaaat
    63361 ccggcctaag cttttcggac aggatttgct aaatgccaaa aacggatttg tggcatctca
    63421 aaccggtatg atcaagggtt cctttataga caatgagaaa atatttgtga tgtcaagaga
    63481 cggtgtattt gagaacagta ccgcctggaa gctggatacc cgtgaacctg ttgacccgga
    63541 attctgcaga aatggctatg aaagggcact gagagagata aaaaaatctg aatatattct
    63601 ggcaaatgat cttattcatg agtttatcac attgggtaaa agccatacaa ctttcagcag
    63661 tgctatagag gccgcttcgg gctatataga aaggattgcc gcacttttca agagtattgt
    63721 tgaatagaag cagaatgcga cgcggacatg ggggggacac ggggacgttt ctcccccatg
    63781 agcaaaagga ataatttact tgaagcaagc attgtcaaag catgatagaa tttacttgag
    63841 tatacccata ttcagcttat caaaaaaatc ggaggattat tattatgaag aaatctattg
    63901 tcctgctttc tatcctggcc ttgctggctg ccgtgtttgc agctgttgcg gcaaagcctt
    63961 tgaaaatagg cgatgttatt gggaaaatct attatactga tataaaaacc tatattgacg
    64021 gaaaactcat tgaatcggta aatattggcg ggaggacaat gatatcggca gaggatttgc
    64081 agtatatggg ctatcttgta gaatggaacg atactgcaag aacactgaat attacatcaa
    64141 acacatccca taatatttcg ggaaaggtca taatacctga cgggaaagtc gcccccaagg
    64201 gtggtttgaa tgttgaaatc gctgccttta tcaatccaaa ccctgaccct tcggttataa
    64261 gccgtggtta tggccctgag ttcggcaagg aaattagact tgtcattcct gaaggccata
    64321 gcagcataga atatataata tccgtaccat ataaattcga accgcgttat ataactgcaa
    64381 tacttcccgg cggtgaatcg ggagaacctg aaaagttgcc tgatattaag ccatactatg
    64441 tactccgctt cagatactca tattatctgg gaaatcataa agttgaacac ggagatttca
    64501 tggaaggcta tcttgatatg agcaccggcg ataaaacagg cgttgactat acctttgaca
    64561 atattgattt gaaataaacg atacaagcta aaaggagcag cgctaatatt ctaatgctgc
    64621 tcctttgtcc atagaataat tcttcattat tgccccggat cctgcttatc ctttctttta
    64681 aatcggcgaa ggggttttcg aaaaaggaag taaacagcag ctataaaggg tgatatcggc
    64741 aatataaatg caatcaggct gagaagagat gtaacaatat ttattaatag gtctatacct
    64801 cttttcaatg caagctgcaa tttgtatcca agactgtcct ggctggcaga atcaatagaa
    64861 atctggctct tttcaaacct tatccgaact tcgctgtatg ttatctgacg atcaaggtta
    64921 gttatatagc cctttaatga ttcaagctca tcagtaagac gccgtatctc cctttcaatt
    64981 tccaatatct cagaaacctt ccagcccggt ttttgcaata gactgcgcat tatttcaatt
    65041 tctgcttcca gattttttat tctaacctga gtatcaaaat agctgtctgt tacatcttcg
    65101 gttttcttgt tactgttaat agttttcccc aaacttctaa ggctcgaaac aaatgtatcg
    65161 gcttctcctg ccggtatcat aatagtgagc cggcctgagg attcgctttg ataagactca
    65221 cgggtgtagc cgcctgaagc cgctgccatt gcctcagcat caagcatgct tttttcaata
    65281 ttatccaccc tcatatccat agacagcatg actatcagct tgcggtccgg cgtctgtgaa
    65341 ggtgctgttg ccggcatttc gcctgctgtt ttttgttctt ccgctgctga ggaagccgtt
    65401 gatggttccg gaggtgcaga ttcatttgca gctttgccgc tgcagcctgc tgaaataata
    65461 cttatgataa taagaatagt aaaaaggatt aaccccaaag accttttttt cataatatca
    65521 ccccgcatat ttaatgatca ccgttcatat ttcaattgtt ggatttgcaa attagacgga
    65581 ttattccata ttaagttgct tacttatgat atattttact ttattttcat aaatattgaa
    65641 atgcctgaaa agaacagtct tggtaatatt tgcacttaag atgtttctca tgtgagtttc
    65701 gagcaaaaaa aagatagcca accaaatggc tatccattct gatagtctca tttcaaataa
    65761 gatcatcttc ttcatccggc agctcggcgt tgtggtagac gtcctggacg tcatcgttgt
    65821 agtcgaacat atccagcatc ttgtttattt tctttactgc ttcagggtct gtaacttcga
    65881 tataattctg cggtaccatt gtcacttctg ccgaagccat gggtattccc gcttcttcaa
    65941 gcttctgcct tacctcggag aagctttcgg gtgaagtaac tatctcaaag gaatcctctt
    66001 ccgcattgaa gtcttcagcc cctgcctcca gagcatgcat catcagttct tcctcgtctg
    66061 tcgtaccatc gttttctact atgattatac cctttttatc aaacatccag cttacagccc
    66121 cactaactcc catgctgccg ccattcttgt cgaatatgtg tctcacgtcc cctgctgtcc
    66181 tgttcctatt ttctgtaagt gctgttacta taactgctac tccccccgga gcataaccct
    66241 cgtagactat ttcttcatag tccacgcctt ccatctcacc ggagcccttc tttatagccc
    66301 tttgtatagt atcattaggc atattgttgg accttgcctt ggctataaca tcggcaagct
    66361 tgccgttggt gcttgggtcg gcgccattct ttgcagccat ggcaatttcc ttgccaagct
    66421 tagtgaatat cttacccctc atggcatccg ccttgccttt tttatgttta atatttgccc
    66481 actttgaatg tccggacata tataaacaac ctccccaatc atagaataaa tcgcaattat
    66541 tatatcatag aatctttcac taatgcaaaa aacttctcta atcttgcttt tcagatggta
    66601 caaatcttgg tatcatcgtt ctcttatgct cgcttctctt attcatggca tccacctttt
    66661 tctttatatg ctcctctgcg gctccggtaa gtatgtaatt gtcaagctca gtataggtca
    66721 tacccatctc actctcatcg gtttgattct cccaaaggcc tgccgtaggc ggctttataa
    66781 tgactgcttc cggaatgccc agcttatagg ccagctctac tacttcgctt tttacgaaac
    66841 tggtcaaagg catgatgtct gctcctccgt cgccgtattt tgtaaagtag cctatagtaa
    66901 attcacatct gttccctgtt ccggacacaa gataatttct taatccggca taataataca
    66961 atgtaatcat tctgagcctt gacttgatat ttgcaactgc aagccttgga tcgctttccc
    67021 ggactccaag cacagccttc atagaatcaa aggctttatc aagaaccacc gtctttgttt
    67081 ccagtcctat agcttcagct acggcaattg catgctcctc atcaatagga ttgctgtagc
    67141 agggcattat tactcccagc gaatcggaag gatatgcttt tttgcataat gcagccacaa
    67201 ccgcagagtc tatgccccct gaaaggccaa atacaacacc ctttgcacct gctccttcaa
    67261 cggtgttttt tatccattcc actgcttcat ttatcactgt atcagcattt atcatttgtg
    67321 tacctccaac aaaacaatag tttttcaatc attactgtag ttatttccta tctataaata
    67381 ttataatagc attcataaaa aatgatgtaa ataatagtta tagtttagtg caaatatcgt
    67441 tatactccaa gtgttcctta ttgagaattt ttacatgcaa taataaaacg tgtaatgcag
    67501 agattaatac tgggaaacaa taagtaaaat caaagtcatg atgtatcgga gctattatga
    67561 aaaataaaaa ccatctgaaa actgcattaa tcggtttcgc agcaggtctt gtcaatggta
    67621 tattcggttc cggaggcggt actattctgg ttccgtgtct tgtattcctc atgggcattg
    67681 aagatcacaa ggcccatgca actgcaattt ccataatact tcccatatct cttatcagtt
    67741 catttatata tttcaggtac gatgtgacgg atctttctct taccctcaag gttgctgccg
    67801 gttcagtcct tggagcatta ttcggctcct ttgttttgaa ctgggttccc atcaataccc
    67861 tcagaaagat attcggcatg ttcatgatac ttgccgccat aaggatggtg ttttgatgct
    67921 tacggtacta ataggctttg cgtcaggaat aataagcggt atgggtatag ggggaggaac
    67981 cttgctgata cccgcattgg tttttatagt aggtaccaag cagcaaatag cccagagcat
    68041 aaacctgctg gtgtttatac cctctgcctc agcagctctg tttgtgcatg cgagaaagca
    68101 caatattgaa aaaggcttgc tctttaagct tgcagtcaca ggctgtgcag gggctgttat
    68161 cggctccctg gctgcagtaa atctggactc cggcattttg aaaaaaatat tcgggacatt
    68221 cctgtttata atgggaatat atgaaatatc ctccaagagc agagagaaaa ataaaaagac
    68281 cggaaaggag ggctgcagat gaaaatcagg aaattgctgg ctgcttatgc tttggcgaga
    68341 ggcgtgatga aataccgccg gagcaggcag aacagtcctg ccgataaatt cgaaaaatgc
    68401 gaaaactatt acagatgtga tgatgatgaa aaggacggaa gaggaaacta ttaaatcagc
    68461 ccccttccaa tttgccctca tattgaagta ttattattga acccattatc agcagtatcc
    68521 cggcaagttg aacaagcccc attctttcac ccagcacaaa aaatgaaagc aatattgtta
    68581 tcggaagctc tgaggtcgca actatgcttg ccttatccgc tccgattaat tttatgcctt
    68641 tgtaaaggaa tataacgggc agtatccctg aaaccactgc aagctcaaaa atatatatca
    68701 aaagctccga agtcattaaa aactcaaacc tgaaaaaacc cggatgaagt atcaatgaaa
    68761 ctgccaaagt tgtgagattt gtatagaaag caataactaa aggctcaaaa tctttaagct
    68821 tcacatcagc atatatattg taaaaagcat aggtacagct ggacattatt ccaaacacaa
    68881 atccgataaa aggagttcct gcaataccaa tattgaacac attaataacc ataatacttc
    68941 caaaaaaagc agtcagtaat gctaaattgc tcctgcgcgt aatcttcctg gtttttgtag
    69001 ccatgaagta aatacttacc agcacaggat gggtaaacag cagcattgaa gcaattcccg
    69061 cattcaattt ctccagtgcc agatagaaaa gaactgtcgt acacgagctt ccaatcaatc
    69121 cctgtgtcag aaccgccttt aagctttcct tcgggaggct gagctttctt atacccgcta
    69181 taacaaaata aataagcata aaaacaaatg atgcagtata ctgcaatacg agcaagtcaa
    69241 aaattttgat cccgctattt attacagtct tgccgataac agctatcaat gcaaagaaaa
    69301 atgctgccaa taaaacataa acatatccca ctttatttct gttcaaagaa taacccccat
    69361 tactaaataa cgtacttttt taatcttccg aagcttattt catcaaccac agcctttata
    69421 cggattccat cctctccgta ctgctcctct gttacttcat cagcactttt atatatttca
    69481 gaaaccacac tgccctcggc gtaggggatc atcagttcta ctgtccttga ctgcttagct
    69541 gcaaagcctt ctatcaatgc gagcagcttg ggcagacctg tcctctcgga agcagaaata
    69601 agcaccgtat tttccatatc ctcatactcc tccgggctga atctgccggg gcatttatca
    69661 attttgttta tagccataat cgaagctttg tcccctgctc ccaactctga aagaacatcg
    69721 ataaccaccc ttgcctgatc ttcataatca gaattgcttc catccactac atgtatcaga
    69781 agatccgcat aaacctcctc ttccagagtt gacttaaagg cttcaatcaa atcatgaggc
    69841 agctttctta taaaaccgac agtgtcagtt accagcactt cctttccgtt tcccagcgtt
    69901 acccttctgg tagtgggatc caaagtggca aacaactgat cttcggcata tatgtcggca
    69961 tccgcaaggc tgttaagcag cgtagatttg cctgcatttg tatatcccac aaggcatacc
    70021 tgaaaggcct ttccggaaac cctgccttcc cgctgaaggt ccctatgctt ttttaactgc
    70081 ttaagctcgt tttccagatc gttaagcctg tctcttattc gccttctgtc cacctccagc
    70141 tttttctccc cggggcccct ggttccgatg cctccccctg ttcttgacat ttgctgcccc
    70201 aatccgatta atcgcggcat catgtatttc agctgcgcca gctctacctg aagcttgcct
    70261 tccctggatc ttgccctctg tgcgaatata tccagtatca gctgtgtcct gtctatcact
    70321 tttagaccaa gggcttcttc aaggttcctg atctgtgcgc cggaaagctc ctcatcaaat
    70381 accgcaagat ttgcctccac tgcctggcag agaagcataa gctccttaag ctttcctttc
    70441 cctatgaaaa agacggtatc ctttttatcc ctgctctgaa ccatcctgcc aacctcgcag
    70501 cctcctgccg ttttgaggag ttctccaagc tcctccaaag cctcctcatc ctctaatccg
    70561 acaagcactg ctcgttctgc atcgatgtca acctctttgc tcgcttttct aatattcttt
    70621 ataccggctt ctgcttcaac aattatgtca aaaatattta ccttcataag cctgttatag
    70681 ctatatggtc ccataacaac tgcctggaac tgtttatcac tgtctacccc aagaaaagcc
    70741 atagtggcat tggtcatttc cccgttggaa actcctactg ccgccataat gtcaaacttc
    70801 attcttttta aggcagagat atccacctct gaaagtcctc cgtgcccatt gggatgggtg
    70861 tgcaggcatc ttattccgct taagctttct tctgaccttc tgaaattaaa atccttcaga
    70921 gtaactgtgt tttcatctcc gatacttaca tcaagaaccg tgccttttct gtctatatat
    70981 actgctatct ccttgccggt ttcttctgtg atatcgcaga tttccgcaat tatttcctcc
    71041 gaaaccaatt gatatttctc aacagttgat tctttaagat tttcaagcct ttttaacaat
    71101 gactttttaa ttcctatcgt gttttccata taatcacctt tgctccagta gtatgctttt
    71161 agatatttta acacacaata atgttcaata tgacgaaata caaaaaatat ttcacagctt
    71221 tttagtgtta ccctctcgct tattattttg acggataaat ttttttgcat ttttcatgcc
    71281 gccgacactc ccgaacatgg cattgagctt ttgagtttca atttgtttga aggagaggcc
    71341 atcatagcag gtttcccgtt ttagcttatg atagctttgg agtcgccttt cagtaagtgt
    71401 cccatcctct atcgccttcc tgacggcaca atccggttct gtggtatgtg tacagtctcg
    71461 aaaacgacat ttcattgcca aatcgtctat gtcagaaaag gatttgctca aatccactga
    71521 ttccaaacca agttccctca tgccgggagt gtcaatgact attcctccct gctgcaaaat
    71581 catcagctct ctgcgggtag ttgtatgtct gcctctatca tcttttctga ttcccgaagt
    71641 catcaaaagc tcctctcccg caagcctgtt tatcagagta gatttcccta ctccagagga
    71701 acctataaaa gatgcagtca tcccaggctg cagccaaggc agtagcttat cgcatgaagc
    71761 ccgatcgttg cttgaagtta ctaccacatc aatacccggt gccacagctg atatctcaga
    71821 caaaggaaca gacaaatctt cacaaaggtc agcttttgtt aacactacca ccggtgtagc
    71881 acagctgttc caagccatgg agatatagcg ttcaagtcga ttcagattgt agtcattgtt
    71941 caaggacatg catataaaca ctgtgtcaat gttagcagca actacctgtg tctgatgctc
    72001 tgtgcctgct gctgcacgct gaaaggcgct tatccttgtc agtaccctat gaataatggc
    72061 attactcgaa ctacctaaac tgagatcaac cataacaaaa tcacccaccg caggatattc
    72121 agataacccc tttgcctcat aacggaactt accggaaatc tcagccagga aatcacctgt
    72181 ttcagtgacg agcttataaa gattcttata ctgcgatacc actcttgcaa gctttaaatc
    72241 aggataaagg gctgcctcgt taaaatagcg ttcccttgca ccgtaactaa ttaaattatt
    72301 cataatattt cacctcgtta tttgagaata aaaagagcat agtcacataa ctatgctccg
    72361 acagtctcta ataaaaaaac tgtttgtcat agtcctgtat gaacatagtt gcacagtaac
    72421 aaaacgaccg aatatcgccg tttagataat gcaactttta ttcattacag aacaatctcc
    72481 acaaagacac cccttttatt cgtttatata attaaattag cacaatcggt taatagggtc
    72541 aagtaaaaac tctgttactc ccattgaaag aagcgaatag aaatgcctgt gcaaattact
    72601 gcaagcacaa tcatcacaat aacaggggcc agaacactgt ctattggtaa accaagcgaa
    72661 gttgctttga ggatttttat cccctgtgta agcggcatta tgtctgctgc tttttgaaga
    72721 gtcacaggca tgatttcata aggcaaagta gcacctgaga aaatgagcat cggaaagtat
    72781 agcaggcttg ctattatacc cgccatcttc atgtttggcg ctattcctcc gacaagtact
    72841 cctatactga acatagacac cataactatc aggtatgctc ctaaaaactg taaccatgaa
    72901 ccgtttaaat ggtagttgaa aaagatcgct gcagtagcat aaactaaaat tagtgaagcg
    72961 aaagaataaa cagcatatat aacaacctgt actgctaaaa tgactgtagg gctgattgga
    73021 gtaaccttga accgctttaa gattttcttg ctacgataat cggagataac taagggaagc
    73081 cccatcacac ctccggcgca tatggctatt gttgctactg caccaaagga ttgttccaag
    73141 aagctatagc ttgcgccatc gaaggcaggc ttgtttccaa aaatcgagcc taaaataatt
    73201 actgctacga caggcatgca aattgcaaaa ataaacatgt ccatccctcg aagggacaat
    73261 ttgaattctg ttttaaaaag tgttttaaat gccttcattt tcttcacctc tctcttctgt
    73321 ataccaaaga tacgcatctt caaatttttc atgtggagtt gaagcaacag cttcctgtat
    73381 agttccgtaa aaaatgcttt tcccgttttt taaaatcata attttatcgc aaagagcttc
    73441 cacctcatcc atgaaatgag atgtcaataa aatagtcagc cctttctctt tcaggctgga
    73501 caaactattc catacatccc gccttgctcg ggtgtcaagt ccggtggtta actcatctaa
    73561 aaacacaacc tcgggatttg ggattaaagc caaaacaata aacaatcgct gtttttggcc
    73621 tccggaaagc tcacttacag ggcttttcaa cttctctgaa atcccgaatt gttttagcaa
    73681 gttttcataa tccaaaggag ttttataaag agacgccgtg atctcacata gctccataac
    73741 tcttatttta tcttggtaat ttgcttcttg aaattgaact ccaacccttt caaatagtat
    73801 ttttcgatct ttctgcggat tcattcctaa aattgatact actccgctgt cttgttttac
    73861 agtgcctaaa atacactcaa tggtagtgct tttgcctgca ccgtttgcgc ccaacaatcc
    73921 gaagaattct cctcgtctaa cagcaatatc caaattctta accgctgtaa cattagagta
    73981 agacttgctt aacttctcta ctttgattac tgattccatt cgatcaaatc cctccattct
    74041 tttctaaaaa aagaataaca cctgagaaaa ctctggtgtt atagtgtagg gtttgaaata
    74101 aaaagttttt tcattttatc aaggtgagtc agcataacaa aggcattttt ctcagcatac
    74161 ctcaaggatg taagcagctt gtcacataca gaaataatgt catcgtcctt tggaggcaca
    74221 tcaattatct cccgtatatc agtctcaggg ttattggata aagcattcat catccttaaa
    74281 attgctgcaa gagaataatt tgcacagcgt aaagtgcgta taatttgcag tcgtttaatg
    74341 tcttcagaag tatatactcg atagccattt tgttttcttc ttgctgtcaa caatccgttc
    74401 atttcccaat ttctcagagt atccatagtg attagcagat gattaaggac ttcttttcgc
    74461 gtaaaaagca tatcctctga ggctttatcc tcccctgata ataacatttc ggtaactgct
    74521 atggtttctt cggctttttg cagttcactt tttatttgct tcaaataaca atttgtaaga
    74581 taaattgctc tatcaaaatc tttggcagca gaggttttta tgatattaat agcctgcttc
    74641 ctgagaccat tttgcagaac ctcgactttt agagcagttc tcgcaaatct aaattgttca
    74701 atatgaaaat cagtaaagat ccgataacca tttggtttcc gttcaggttt gggaataagt
    74761 cccaattctt cataaagtct aactgtgtta ggatgaatac caatgaattt cgctatttct
    74821 gcagttttat aggtgttcat gctatcagag cacctccgtt ctaatatatt atagcattat
    74881 tgttacatat aagtctgggt tattctataa atgcatgtgc caacgggtaa aaaaataaca
    74941 gcctcagctg ttatttttat tacctggcgg cgatctactc ttccgggacc ctgcggtccg
    75001 agtaccatcg acac
//
LOCUS       DAOOAB010000007        74464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_411_length_74464_cov_14.255359, whole genome shotgun sequence.
ACCESSION   DAOOAB010000007 DAOOAB010000000
VERSION     DAOOAB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 74464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74464
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_74464_cov_14.255359"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKU88_02740"
     CDS             <1..215
                     /locus_tag="PKU88_02740"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104003.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPX68234.1"
                     /translation="GRSAIIEGCEKFSGAKVRATDLRAGAALVIAGLCAEGRTEIHDV
                     YHLDRGYIRIEEKLKKLGADIRREEE"
     gene            655..1614
                     /gene="spoIID"
                     /locus_tag="PKU88_02745"
     CDS             655..1614
                     /gene="spoIID"
                     /locus_tag="PKU88_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HPX68235.1"
                     /translation="MKKLAAAIAVLMILLFAIPLLLVRDKGEESGKKEVPREDLPIEV
                     YMCDTDEVVTMNLEDYILGVVAGEMPASFHPEALKAQALAARTYTMLRMRQFGGSGCS
                     KHQGAEICTDSTHCQAYRDPASVKKDLDKLKDAVYGTAGEVIVYDNKLIDAVFHSTSG
                     GKTENSEDIWSNRVPYLRSVVSQYEEHSPKYVARQEISIDNFVQCIKKLDSGVVINKK
                     DIKNEIKVLERSEGGRITRIKIGNKTFKGTDVRNTLGLNSSNFNFDVTASTVLFTVVG
                     NGHGIGMSQYGADGMAKNGASYRDIVTHYYQGVAIVSIKDLTN"
     gene            1681..2463
                     /locus_tag="PKU88_02750"
     CDS             1681..2463
                     /locus_tag="PKU88_02750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX68236.1"
                     /translation="MKFRKEFNNRHIYFLVLLIAALLTAGGVMQYRNYLLDKQAQLDF
                     EKSMEEYIEESEPSSTAQVIEEKAESGGSENVVETPDATEVSNSSSVKITEDKKDTKE
                     KVNEAKPISAIKMEEPAMETMVVPVFGTMYTEFSEDVLVYSKTLDQWSTHAGIDIKAE
                     EGSPVRAAMDGVVDQVVNDPQLGLTIVLDHGGEVYTKYSNLSTLDLVSINQSVKKGDV
                     ISGVGKTALYEIADDPHLHFEVIKQGKSVDPKKYLPKQALKK"
     gene            2572..2826
                     /gene="spoIIID"
                     /locus_tag="PKU88_02755"
     CDS             2572..2826
                     /gene="spoIIID"
                     /locus_tag="PKU88_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HPX68237.1"
                     /translation="MKDYIEERALEIAKYIISSKTTVRDAAKIFGVSKSTVHKDVTER
                     LPRINPSVALQVKEIMDQNKAERHIRGGRATKMKYQSISL"
     gene            3059..4093
                     /locus_tag="PKU88_02760"
     CDS             3059..4093
                     /locus_tag="PKU88_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPX68238.1"
                     /translation="MWGGQDIGIDMGTASVLVYIKGKGIVLREPSVVAIDRNTNKILA
                     VGEEARLMLGRTPGNIVAIRPLREGVISDYDITEKMLRYFIDKAIGRRRIIKPRMVVC
                     VPSGVTEVEKRAVIDAANNAGARTTRLIEEPIASAIGAGLDISKASGNMVLDIGGGTT
                     DIAVISLGGIVVSTSIKIAGDKFDEAIVRYMRKKHNIMIGERTAEELKINAGTAFPRE
                     EQLKVEVRGRNLISGLPKTIEVTSDEMLEALSEPVSAIADAVHSVLERTPPELSADIS
                     DRGIVMAGGGSLLNGLDKLISKRTGIPVYVAEDAISCVAIGTGQALEHIDMLQDSDIN
                     ANRKIITLNY"
     gene            4267..5040
                     /gene="flgF"
                     /locus_tag="PKU88_02765"
     CDS             4267..5040
                     /gene="flgF"
                     /locus_tag="PKU88_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HPX68239.1"
                     /translation="MIRGLYISASSAITEAKRVDVIANNIANVNTTGYKKDVMVTQNF
                     SEVLISRINDRFGKDLKVSRPFKPLGPLSYGIHSSEIMIDFKQGQLNSTGNSFDLALR
                     GKGFFCIDTPEGERYTRNGSFAKDAEGWLVTGEGWRVFGEDGYIRIEGSDMTVNEKGE
                     VFSDAQLAGKLRLVDFEDYEALRKEGNGLIRIAEEIEAEPVITAGTVQQGFLEGSNVN
                     SVKEMVEMLTMLRTYEANQRIIKIHDELIGKAVNEIARV"
     gene            5054..5848
                     /gene="flgG"
                     /locus_tag="PKU88_02770"
     CDS             5054..5848
                     /gene="flgG"
                     /locus_tag="PKU88_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HPX68240.1"
                     /translation="MMRALWTASTGMLAQQLNVDTISNNMANVNSVSYKKSRVEFKDL
                     LYETLERGIVRDGQGRPVNLQVGHGVMPAASVKFFTTGNLASTENPYDVAIEGLGFFK
                     VLGPEGQEYYTKDGAFKISPDGENFNLVTSEGYYVQGENGNIELGSDIKDIIIDARGL
                     ITVKREDGTIEELGSLSISTFVNPAGLESVGKNLYVATVASGEAFDTQNDGTGGNILQ
                     GYLEYSNVQIVEEMVNLIQAQRAYEMNSKSIQTADEMLQMANNLRR"
     gene            5851..6225
                     /locus_tag="PKU88_02775"
     CDS             5851..6225
                     /locus_tag="PKU88_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HPX68241.1"
                     /translation="MINPINNNGITDYNNAKSKAQEAKQGEFGKALEKAMEEKDEKKL
                     KQACSDLEAIFVSMVFKQMRSTVQKAGLIDGGMAEEMYEEMLYDKYAEEASKGEGIGL
                     ADLLYQQLSKSLKPEREREDVE"
     gene            6215..6643
                     /gene="fabZ"
                     /locus_tag="PKU88_02780"
     CDS             6215..6643
                     /gene="fabZ"
                     /locus_tag="PKU88_02780"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPX68242.1"
                     /translation="MLNNTDIQGILPHRYPFLLVDKITEIEYGKKAVGIKNVTVNEPF
                     FQGHFPGLPIMPGVLIVEAMAQVGAVAILGMEENKNKLAVFTGIDGMRFRRQVVPGDT
                     LRMEVEMLNFKRGIGKAKAQAYVGDELAASGELMFAIVNK"
     gene            complement(6704..7258)
                     /gene="yyaC"
                     /locus_tag="PKU88_02785"
     CDS             complement(6704..7258)
                     /gene="yyaC"
                     /locus_tag="PKU88_02785"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HPX68243.1"
                     /translation="MIEKYSIKDGICVHHTDNRAHLKISDFLKSNKLDKCLILCIGTD
                     RFIGDSLGPLTGTLLSKLSLPVPILGTLDNPIHAVNLRRVMSHIKTSYKDHKIIAIDA
                     CLGAGKSVGYVHVKEGSIQPGKGVGKRLSHVGDFSIIGVVDEAVESGDSEYENYLKIS
                     NVRLGLVMKMSETITKGIYAAFRQ"
     gene            7570..7645
                     /locus_tag="PKU88_02790"
     tRNA            7570..7645
                     /locus_tag="PKU88_02790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7603..7605,aa:Pro,seq:tgg)
     gene            7660..7734
                     /locus_tag="PKU88_02795"
     tRNA            7660..7734
                     /locus_tag="PKU88_02795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7693..7695,aa:Glu,seq:ttc)
     gene            7879..7954
                     /locus_tag="PKU88_02800"
     tRNA            7879..7954
                     /locus_tag="PKU88_02800"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7912..7914,aa:Thr,seq:tgt)
     gene            7959..8043
                     /locus_tag="PKU88_02805"
     tRNA            7959..8043
                     /locus_tag="PKU88_02805"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7993..7995,aa:Tyr,seq:gta)
     gene            8252..9004
                     /locus_tag="PKU88_02810"
     CDS             8252..9004
                     /locus_tag="PKU88_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPX68244.1"
                     /translation="MARQARKRSATGVYHVMLRGIDKRDIFLHDEDKMIFMKKLLKAK
                     ENAKFGLYGYCLMDNHIHLLILESEEIGVSIKRVSVGYASWHNNRYGRVGHLFQNRYL
                     SEPVESEGYLVHVLRYIHQNPLKANMVKKPEDYPWSSYNDYISAYQGNGVNIDADMIM
                     AYFKDQNDFEEFTNTQNDDIFVDFEPEEKFTDEALGDIIKKKIGSVFLYGKSIGDRNR
                     LIKDIYNSTGASIRQLERVFGVNKNIIAKAVK"
     gene            9059..>9245
                     /locus_tag="PKU88_02815"
     CDS             9059..>9245
                     /locus_tag="PKU88_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68245.1"
                     /translation="MEFIPAKTIISGYTENNPWFGNNYSMNIYKGCCHGCIYCDSRSE
                     DLLNSIKRHSPVLVKHTV"
     assembly_gap    9246..9345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9346..9861
                     /locus_tag="PKU88_02820"
     CDS             <9346..9861
                     /locus_tag="PKU88_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX68246.1"
                     /translation="DLLNSIKRHSPVLVKHTVTTADDSLCSKIEPNAPAASRRFEAVR
                     DLSMNGIFTGILLMPVLPFLEDNEDNISNIIDLAYEKGAKFIYPAFGVTLRQNQREWF
                     YSKIDEHFPSIRQKYVKQYGNSYKCISPRAKELWKFFRARCDRLGILYKMADIINEYQ
                     QNYLYEQISMF"
     regulatory      9965..10071
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            10157..11347
                     /gene="metK"
                     /locus_tag="PKU88_02825"
     CDS             10157..11347
                     /gene="metK"
                     /locus_tag="PKU88_02825"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPX68247.1"
                     /translation="MLKKLFTSESVTEGHPDKICDQISDAVLDAILEQDSMGRVACEV
                     SVTTGLVLVMGEISTECYVDIPKIVRKTIEDIGYTRAKYGFDCDTCGVITSIDEQSSD
                     IALGVDKALEAKKGEMTEQDIAATGAGDQGMMFGFACDETPELMPMPISLAHKLALRL
                     SQVRKEGILDYLRPDGKTQVTVEYDDGRPVRVDTIVVSTQHSPEVDHDTIERDIIDNV
                     IKPVIPEGMLDKDTKFYINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYARHGGGAFSG
                     KDPTKVDRSASYAARYVAKNVVAAGLASKCEVQVAYAIGMAKPVSVMVETFGTGKLTD
                     DEIAKIIMKVFDLRPAAIIKDLDLRRPIYRQLAAYGHMGRTDIDVPWEKTDKVEELKK
                     LAGI"
     gene            11352..13556
                     /locus_tag="PKU88_02830"
     CDS             11352..13556
                     /locus_tag="PKU88_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPX68248.1"
                     /translation="MDEVQGTICEIIFRNEYNGYTVLDLECNNELHTLIGYFSFLNIG
                     EIIKAYGSWVQHPSYGSQFKVETYTTVTPATINGIEKYLSSGMIPGIGPHTAKKLVEK
                     FGLDTLDIMQYNPDRLTEVEGIGDKKAAKISEAFQEQKELKDIMMFLEQYGIGPAYAV
                     RIYRTYGANTIKEIKENPYKLADDIFGIGFKMADRIAISMGVSKFSEYRTASGTRYVL
                     NQYHGNGHTFVPQEELVRSSAALLDVDEAHIEDTIVRLFIDNKLVAENIGDIRGVYSV
                     PFFRAESGTARRLMQLLYYKIPPMDLDMEGEIAEIEKAEGIELADKQKTAVKEALTQG
                     ILVITGGPGTGKTTTIKTIISIFEKHGLEVLLAAPTGRAAKRMQEATGREAKTIHRLL
                     EFGYGDSDEEMFFQKNEESPLECDVIIIDEVSMIDILLANNLLKAIAEGTRVILVGDV
                     DQLPSVGPGNVLRDIIDSGVLPVVRLDEIFRQAESSMIVINAHRINRGEPPVLSSKNG
                     DFFLIRQESQEDIVATIIELCTSRLPKYTGLDPYEGIQVLSPMKKGLCGVLNLNHVLQ
                     EVLNPRGSGKEEKQHREAVFRVGDKVMQIKNNYRMRWRNINNLETEGEGVFNGDLGTI
                     VSIDNEELYMEVVFDRERKVKYDFTMLDELEHAYALTVHKSQGSEFPVLVMPLTFGPP
                     MLMTRNLLYTALTRAKSMVVLVGKERFLYQMISNNHIITRHSGLRKRLSAAE"
     gene            13572..14276
                     /locus_tag="PKU88_02835"
     CDS             13572..14276
                     /locus_tag="PKU88_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPX68249.1"
                     /translation="MIKYLLDLLYPPKPKCSICGRAGRLAVCNACMASLDYLQGITCL
                     HCGKQLNEQYDDCTCPDCKTGIFYYERAYSCFEYGGMGKKLIHKLKYEGMVQLANVIA
                     GLMAERLENERLVIDAIVPVPIHKKKLEARGFNQSQIIARELGERLHKPVMGCLERMV
                     ETKEQYNLDRNQRFLNIRGAFSVKLSYNIGKYKNILLVDDIYTTGSTVNECSKLLKDA
                     GAAKVYVITAATGSNT"
     gene            14320..14745
                     /locus_tag="PKU88_02840"
     CDS             14320..14745
                     /locus_tag="PKU88_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPX68250.1"
                     /translation="MAEIRNCPRCGKIFTYIGKPICGRCIESEENEFKVVKEYIYDNP
                     GATISEVSQETEVSVEKIMRFLREERLEIKSENSNLMLECERCGRAIKTGRFCDYCKE
                     QINKDFKREFGLDRKEPEPRPQTRAEKERMYTATRRKND"
     gene            14880..15185
                     /gene="flgM"
                     /locus_tag="PKU88_02845"
     CDS             14880..15185
                     /gene="flgM"
                     /locus_tag="PKU88_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPX68251.1"
                     /translation="MGFNINKIDGVSGVYKNQKVASRKDVPESTRARGKKDEVQISKE
                     AMDFQLVMKAAKAAREIPDIREEVVAPIKEKLDNGTYEVDSKGIADKLLARRLNLKI"
     gene            15247..15759
                     /locus_tag="PKU88_02850"
     CDS             15247..15759
                     /locus_tag="PKU88_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPX68252.1"
                     /translation="MINVDKEVNAVIDILAEEYGYYSDLVELSKSKKDIIVAGKVAEL
                     DKMVKLEQDMIFSIGQLERKREDVISMLCKALDMNSERVTVHELAKVLKPELKNKLED
                     IQSKLQKALSELKAANDINGQLIEQSLEYIDYSINLIAGSGMETGSLYEDIGKNKSKQ
                     GKKNIIDTKV"
     gene            15825..17267
                     /gene="flgK"
                     /locus_tag="PKU88_02855"
     CDS             15825..17267
                     /gene="flgK"
                     /locus_tag="PKU88_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPX68253.1"
                     /translation="MSFRGLSIGVSAVFANQRALDVTGHNISNVNTAGYTRQIISNSN
                     AFYQKLGHSGNGKVMQVGYGVDVQEIRQYRDEFLDNKYKKEKTGLGYWEERYSSVREL
                     ETIFSDNSEDGLQEVMNNFWNSWEQLSKPTGGLTTRAMVKENAIAFVETVKNMDTMLL
                     NFRKSKDKEIIDNAARINEIAKEFATLNLDIKKIESHGVTANDLRDRRNLLIDELSGL
                     AKIQIIEGDYINVSLEGRLLVEGSRYEQIKTVPDTGNSGFVRLVWEIDNGSLYISGGS
                     INSSFESRDVLVGGFRERLDQFVIGIADEINAVHITGYGISDTLQRPFFINYRDNSAV
                     GINLLTIAYNPELENYDRIAAAEDAYSNEDNRIALKIAALRYTDCFTDEKYTTTAADK
                     KYNYDEFYRNIISDLGNIGQEASTAADAQKLLVDQIDYSRQALSAVSLDEEMTNLIKY
                     EHSYNAATRIVNVIDEMIEMIVNRTGLVGR"
     gene            17280..18746
                     /gene="flgK"
                     /locus_tag="PKU88_02860"
     CDS             17280..18746
                     /gene="flgK"
                     /locus_tag="PKU88_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPX68254.1"
                     /translation="MRTTFGSFNIATSGLFASQRSLDTTSHNISNANTDGYSRQISKQ
                     RATMPTYGDPTGVVGTGVETYDIIRMRSSYLDTKYWGQNKTYQEWNVKQTQLENLEGL
                     FNEPSDTGIRIVMDEFFTALEELSKQPGDSTCRVNVVEKANMLTTTINRNGHELVNSI
                     RDVNFSIKNKVSQVNSLTEQIANLNKHIFSFELGGNKANDLRDQRTLLLDELSSIINI
                     TVIEMPGPNNNNYMDVKVGGITLIDHIHFNKLATEDETKEVSDLGGGKISKVVWEGLD
                     NQEVNISSGELRGLLDVRDGNGEDYSYRGIPYYLNKLNEYVKGFTTAFNTQHKMGVDL
                     QGDPGQNFFYEPGIPEVNCISFKVNSDIIADPTTIAASSQGNGESNNDNIKLLIALRE
                     NKGMFSSLIGTPDDFVKSILSALAVDSNQAKRMTTNAQALTDHIYNSRLSESGVSLDE
                     EMTNMVKFQQAYNASARMVTTLDAILDTTVNRLGLVGR"
     gene            18759..20024
                     /gene="flgL"
                     /locus_tag="PKU88_02865"
     CDS             18759..20024
                     /gene="flgL"
                     /locus_tag="PKU88_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HPX68255.1"
                     /translation="MRVTNSMIINNLMRNLNKNLNRMEKTQRSLSSGKKFVNPSDDPI
                     GVSRSLRLNTEVATMNQYKRNADDVLSWLSTTEMAASNITEVLKRAKELTVQAASETN
                     SIDERVSISKEIEELRNQLIQIGNTTYAGSYLFSGFKTDKPLLAKDGTYDLGDSTNIL
                     SSEEIIEVNIGVGDRIGENFVGQRLFGLYSVAADLDLKNAESIKQQQIITGSFLDLAT
                     NPIDTATSFDITYQGATTTINLPNVPYNDIADVEGDISAAIMASADLNGHVTVSVANN
                     KIVFEADGEFSVQTGTAIGISDNKKSVSKVSSGDKSQMIAIFDQLLADLIADDTAGIG
                     NALNRIENQFENVNAITAEIGVRMNRVELTSNRILDDTLNLKDLLSKNEDADIAEVVM
                     NLKMEEYVYQASLSGGAKIIQPSLVDFLR"
     gene            20053..20484
                     /gene="fliW"
                     /locus_tag="PKU88_02870"
     CDS             20053..20484
                     /gene="fliW"
                     /locus_tag="PKU88_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPX68256.1"
                     /translation="MIIKTKFFDEIEISDKDIVDFDEGIPGFPGTHRYAIINDDNTFI
                     SYLQSLDSEDVCFIMIPPVFALKDYDIEISESTVKKLSIERPEDVKLYAILTILGNFK
                     DATANLKAPIVVNVNNNKAVQEILDDERYGIRHRIVKESDV"
     gene            20484..20711
                     /gene="csrA"
                     /locus_tag="PKU88_02875"
     CDS             20484..20711
                     /gene="csrA"
                     /locus_tag="PKU88_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPX68257.1"
                     /translation="MLVLTRKKDEAVVIGDDIEIIISEISEDKVKIAIKAPKNIKIFR
                     KELIKAVEDENRKSVDMSDLDIDGLREAIKK"
     gene            20874..21641
                     /locus_tag="PKU88_02880"
     CDS             20874..21641
                     /locus_tag="PKU88_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPX68258.1"
                     /translation="MRINNNIMAMNAQRQLGVNSTGQSKALEKLSSGLRINRAADDAA
                     GLSISEKMRGQIRGLAQAQRNAEDGISLIQTAEGALNEVSDMLVRMKELGVQKLNGTY
                     SAGDVANMTAELTRLNSAITSIVADTKFNGTQLLKGGTVNLMLKDTGTATAYNLANIG
                     TVPAADADLATIETAINTVSTQRANLGAVQNELEHVVKNLGTTTENLQASESRIRDTD
                     MAKEMMAFTKLNILNQAAISMLAQANQAPQGVLQLLQ"
     gene            21773..22402
                     /locus_tag="PKU88_02885"
     CDS             21773..22402
                     /locus_tag="PKU88_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68259.1"
                     /translation="MQVWFLDRTYNYSNESASVKELFDNIDRLLADPQYILAGMIIDD
                     MEIYENYVEYITKRIDRIKTIKVVIDTVENTIDSILESMSKYMDRAVPELELLAGSFY
                     SGGDEEVSIEKLGQLAEGIQWILNMIYYIDNNKALHSVLKSNSIWNECSSYGNRLGEE
                     FNNLKEAMGNIDMILIADILRYEITEVFTGINQRIKQIYGHGDEGNGNR"
     gene            22389..24275
                     /locus_tag="PKU88_02890"
     CDS             22389..24275
                     /locus_tag="PKU88_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPX68260.1"
                     /translation="MGTDNSAILRTRFESLWESLNKTTENVGPNVVIENARNSMYTLY
                     VENSGNKLYIHSRYNPVNEAEMFAGQFKDVDKYDHVIFYGIGMAYHIEAFFSKFGNKH
                     FSIYEPFLNIFKSFISYRSMDSIPLEYLDNIFIGDSLEDPYECLEALLKEIEGEVLLV
                     ILPSYERIFSQEYKSFYQRFGSILDDTKTLNRTNRLFEKLYVTNIMKNFMEIIDSPNI
                     LHLKNNKFAGNPAILVAAGPSLEYELKNLRYIKKKGLAYVLSVGSAINTLLEYDIYPD
                     AALTYDPQNHNHIVFKKLKDKGITSIPVVFGTIAGYGTVQAYEGPKLHFISSNDHVAP
                     YFFKPESAKEFDTVHDAGSIAVAAMQMLYKLGCDPIILVGQNLAYKDRKLYAKGIDYG
                     RDLSETDNKYLDSSFMVDDVEGNKIQTNKVLNHYKDEFERCIKQYGIENIINTTRGGA
                     AIGGTVYKPLKDVMENMLVSSIVDAEWYKDEGVSYDIDYMISRCKSIEAEQRHLLDIL
                     KSIKKIAAQINGNTNSVEAPESLFSFFYAEFNKMLQNTYFSTFIYPMNYYQCSLLNRK
                     INSINTEKNIVLKIQKLCREMEPFIDGCINDMHLISPLFGDMYEAIMDNDIKIMTFTI
                     KLCS"
     gene            24412..25386
                     /locus_tag="PKU88_02895"
     CDS             24412..25386
                     /locus_tag="PKU88_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX68261.1"
                     /translation="MFENKKILVIGGTGTIGQALVNKLISYNPEVIRILSRDEFKQYQ
                     MQQQLEGYKNIRFLLGDVRDKERIYKAMHGIDIVFNLAALKHVPSCEYNPFEAVKTNV
                     LGTQNVIECAIENNVKNVIYASSDKAVSPTNTMGATKLLAERLIASADYWKGGKIPVF
                     AAVRFGNVIGSRGSVVPLWEEQIVGRKEMTVTDPNMTRFMISIGSAVNLTLRACEIAK
                     GGEIFVLKMPVIRLGDLAEVVLDKVCSEKGIAKDEVNTRVVGLRPGEKMYEELMTVDE
                     AQYAVEFDDMFAIQHLSNITLPSNKPKEFRSDMCSIVEKSSIWSMLID"
     gene            25410..26396
                     /locus_tag="PKU88_02900"
     CDS             25410..26396
                     /locus_tag="PKU88_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68262.1"
                     /translation="MKNFFIKHKDKILSGQKVLKEYDIFEEIDTRLSDSYLFDRENNC
                     VKLGYSRCKKIGFDNLLLYRFGTVSRDDETLIFVPQVDEQIGDFIKRSQVFLNNPVLP
                     DETPVIHLNFDIHEKIEACIWLGNKYAYETYDMYLKGIIPWDNERKRSTEFKRTGIFI
                     GTSSIHIIEYSGTEAEHKHMVFSTPNYGNEVDCYIDLKKGYFRLGQNGSKIYHSGLKQ
                     DESCSINVSCNSRDNQVFSCTNPGLILRRFELCRNQGVIYFKSIDASLAKESRVMMLY
                     TGDIAVQYFNEKSNQWHFVKDSILFKDLHNVSLRAIMDTDDRIYALILLKVI"
     gene            26432..27262
                     /gene="kdsA"
                     /locus_tag="PKU88_02905"
     CDS             26432..27262
                     /gene="kdsA"
                     /locus_tag="PKU88_02905"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPX68263.1"
                     /translation="MQPRVVNVCDVKIGTNNPLVLIAGPCVIESEEIVMRTAEYISRL
                     AEKLRVSFIFKSSFDKANRSSITSYRGPGIDNGLKLLDKVKSSFRVPVITDIHSAEQA
                     MPVSDVADIIQIPAFLCRQTDLLTAAARTKKPVNIKKGQFMAPWDMKNVIDKILTYDN
                     TNILLTERGTSFGYNNLVVDMTSIVEMRKFGFPVIFDATHSVQKPGAMGTTSGGNCEY
                     VKYLAQAATAIGIDGLFLEVHPDPNTALSDGPNMIALNKLEELLVTIMELDRTIKGGI
                     "
     gene            27262..27756
                     /locus_tag="PKU88_02910"
     CDS             27262..27756
                     /locus_tag="PKU88_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPX68264.1"
                     /translation="MSRVKMLIMDVDGTLTDGKLYISGSGEEMKAFNVKDGYAIKNIL
                     PSMGIIPVIITGRKSKIVEIRAKELGILEVHQGIENKEETYIKLLDSYSCQDEDIAYM
                     GDDINDIPILKRAGISACPWDASAAVKSVCSYVMSSKGGEGAVSEFISHLHEEITGSK
                     LGVL"
     gene            27821..28693
                     /locus_tag="PKU88_02915"
     CDS             27821..28693
                     /locus_tag="PKU88_02915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX68265.1"
                     /translation="MNFIYNMNNYEIVQCDKCLTSTVLSMPQDDEIEDFYSGFMFCAN
                     ASNKSIFEDETFNKWFDSFNLGCNAKMLDVGGGGGFFSYAFEYFDKGDSYYIDIDNEA
                     CEFAAQELGLKNVINDNVKNLKDRTNLKFDFIYARHLIEHLKDPLEFIDSCIELLTAS
                     GVLIIQFPNGVSFEYLGYPELLKSRVKTIQSSNRNFSRIETLLTICSNKIAHGIDPIR
                     HLWAITPKGISSYLSGKDVDFEIKNAPLTDPVYSPYYKAKGVYNKVRSSIINNSLVKI
                     NGGTHLIVIIKRKQ"
     gene            28726..31752
                     /locus_tag="PKU88_02920"
     CDS             28726..31752
                     /locus_tag="PKU88_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPX68266.1"
                     /translation="MDNNVLLSTLKYNYCIGCGICRMSCPYEAIKMVLTKNYDINPKI
                     DKSKCIKCGICTEYCPHTKEKLLSEIQKVCETSSPNSFGLEKATYLLAHDKDADKRKK
                     SASGGVATALVEHLFKKKIIDGVIHATMRLGSIGELHYEASISNSFVEADRKRSSYYG
                     QIDYSHIVDEIKGKEKTYLVIGVPCAIRALTKLFKEHSSYNKNKIYTLALACSHNVNG
                     QFVDFLAESLGIPKDIQFKANLRNKDNIKDANNFNNHFFNGQQDIVKINRFKSLFTDI
                     WRNYFFSMKVCGYCSDFWGYTADISLKDAWGKWADNPLGDSIAVIRNKKLLDELLDSD
                     NIEYKYLDFETVAGSQLSTTLYKQAKAADRLNKPIWHIDNIRSGYLKHTVLSELSKFC
                     YRHFGYELSKRILFIVLAVLVKLENNARNIENIGFLKRKALIKDITKKADNKRVVFFG
                     TGKMAETLKKAIPVHVEYYVDNDEKKHNTMIDNTIIKDPNVILYENRDDVLIIVASMY
                     IDQISMQLEKMGLIPGVHFIDGRILFENIAGKPKHFKESKFAMGIEVILKPFIAVFIK
                     AKSIFVYEKTKKHKKILVVGGYGYKNTGDEAQLSATIRSLKEEFEGYIIKVLTPNQHY
                     TYFTHGECNVGDAPRVAFYDAGETALYYLDSTYKKLKFIFVSLLVYWNAFFVRAGLPL
                     FLINAKKAALLYEISTSDMIYFSGGGYLTGKTVSRLWDSMFLIRIADIFRVPVFLSGQ
                     TIGVWNGSFNKRLSKWGLSKAKLITLRDSYESVRALEEIGISGEHVFSTFDDALFCEK
                     LEDESKINNLLMNSGIRDEQANKGYVAINIHYWGLDTSEEKRQLLEKIDKVVGIVLRK
                     TDCNILFVPMVPSDEEAINDFMKAYRKDRLYNLSYDYDFRTVRALIGGSDICVTMKHH
                     PIIFALGEKVPVISLALGDYYEHKNIGALGIFGLEKCNIILSDALYYRDFDLILDEIY
                     SAKENIIRKIEESYKEVRQKKAYFISRIKEIISNKL"
     gene            31784..33484
                     /locus_tag="PKU88_02925"
     CDS             31784..33484
                     /locus_tag="PKU88_02925"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HPX68267.1"
                     /translation="MEFDYFNLFNKVKNIIYEAQAKKLVIFGTGSFAKLLSEYMPKKP
                     SYYVDNNINKLNRLFMDSDIHSPDLLKTEDSNNLLILVASMYYEEISQQLKSMGLNEG
                     EHFENGRPLYDLINVYKSFGLEEDLKEYIRKEQLITSSCTDPHKVVLANKECEWLCAD
                     NDRLYLKGLLEGYMLVFVEDNRNTHNIYFEKMDKSFRQIEYKGINLYEVSIYNICVDL
                     QLVITDIDISLNEHKDAIRKWYNISANYINTIENLTESLNVTSVMILQGYLYTSAILR
                     RFALSNEMPILALENTFNKTKAIWDNISGITVNKNLARSYYWRYYDSVDFSTAFEYCM
                     KYIDGIKNLKMEEHITPSNKFLQKSGRKTILYIGQVYSDSSVLFGIWNFKNPVEIIKV
                     LVDYCINNDFQLIVKPHPKEKDLVDVLGNKYDKLTFRMINKDKELAEKITKYGVVVDS
                     ENEYDTYSLIDYSDLCITINSQAGLEALIKNKDVILCGNCSYGGLGFSYEAHNKDMLL
                     CFMDMVLKKGISLINKEDIYKFFYIFNEKYCISKDAEAIIDKLKTIEFGLNKKNAGRN
                     "
     gene            33486..34469
                     /locus_tag="PKU88_02930"
     CDS             33486..34469
                     /locus_tag="PKU88_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPX68268.1"
                     /translation="MNNGLYEGNIRRVIQQEGEKLLQIADNLDYEKVNIAVELLLNCK
                     GKILLMGVGKSGFVARKIAATFTSIGTPAIYLNATDAMHGDIGIVSSSDIAIVISNSG
                     ESHEIIEVLPFIKNRNVSIIALVSNAKSTLAENADIVIDLMVKQEACQYNLVPTTSAA
                     AVTAFGDALALTLMEAKNIGPENYALNHPAGRLGKRLTLRVKDLMHVKESNPTIYGDS
                     NLYEIIKAISDGGLGAVSVIAEDGHLIGIVTDGDIRRAVQNNLPNNIYDLYAEQVMTK
                     DPIKVTQDILAFEAMNIMENRISQISVLPVVDNNNVSIGMIRLHDIVRSGL"
     gene            34469..35197
                     /gene="kdsB"
                     /locus_tag="PKU88_02935"
     CDS             34469..35197
                     /gene="kdsB"
                     /locus_tag="PKU88_02935"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPX68269.1"
                     /translation="MKILAVIPARYNSQRLPGKPLALIGSKPMIQRVYEGTKQCEFFN
                     KVIVATDDDRIKECVESFEGVAMLTDTNHSNGTERVAEVAQRFPEADIIVNIQGDQPF
                     ITSDILTELIHPYLNNQMPDMVTLACPLNEELDYTNPNVVKVICSTSNKALYFSRAAI
                     PYYRTKLEAPVYQHLGLYAFTREFLLKYVRLEPTPLEQCEGLEQLRALEHGYNIVVRI
                     IKESIMEINTPEDLVRTELKKKFI"
     gene            35220..36656
                     /locus_tag="PKU88_02940"
     CDS             35220..36656
                     /locus_tag="PKU88_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX68270.1"
                     /translation="MIFCTSVCANYLPKAMVLAESLKKHNPNSEFVVCLVEKTIEEAA
                     EKFEYFDYVVLSKDIGIRDFDNFIFRHTIVEASTAVKGDLFEYLLNNFKDEDKFVYLD
                     PDIMVYSSFSELDSILEKNEIVLTPHTYFPEAGEEFNNGIFLECNHLKRGVYNLGFLG
                     IRRGEESSKFITWWKNRLNLLCYDEVDKGLFVDQKWIDLAPALFNVYILKEPGYNLAY
                     WNLSKRNISIQEGKDYYANDKPLVFVHFSGWDSGFNNTAIATYAPNVNELIHEINKQY
                     NAQLSEMDKYNLSKAKWSYNYFDNGDEIPRGIRIIYRNSGVLQQLYTHPFGQLNGELG
                     EYLEGLYNIKDNNPEADKWSIIDNYINEVCLLDKEWKAKKRIELGILKLILGGVEKKC
                     IIWGASNGGKIMKKVLDVFYEDVKIAGFIDKYKTGEFEGYNIYKPGQLKELSFDYAFI
                     ATSPGKADAEKYLKSNGCLPYKDYYSLF"
     gene            36694..38376
                     /locus_tag="PKU88_02945"
     CDS             36694..38376
                     /locus_tag="PKU88_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPX68271.1"
                     /translation="MVRSVLIITKCIRMDAEGAKLIKRIKGLIENNISVIVASHGGNG
                     AAEIAEAGAEYYRFRWLNSEPDELDHKTFVENLSIIKGIIDKKQIDIIEVTDLYPALF
                     VYYLSKLVSIPYLVNFLSIEDLLADDFKYIGFVKEINSASILYAADSKLISEVQSHRE
                     ITFSNWKVIHAIDSDLRNLINEYNWVIDNFSTNDSIFLNYDDLHVTSNNSYLRFLEDK
                     DKSLVLFGASEFGLYAQDSLRMNNIMVDYFCDNDVSKCGSIISGAAIISPDQLLALYG
                     RVNILITSMYRAEIERQLLHLGFSESDIYFIPREIYGRLSYYKKSQEAISCSCERIAE
                     ALDILEDIKSKQVFINIIKHKMSFDFNLINEIIDHEQYFDSSILEFCDHETFVDAGAY
                     DGQTTMEFIAKVKGRFNKAFVFEPDKNMLDKVAGTIDSVNKDNNIKLIAGGVYDKSGV
                     IKFTPGEIGGSHISNNGSENIEIYKLDDVLKDEDVTFIKMDIEGAEMNALIGAQNIIR
                     ENKPKLAICTYHMPPQDFWEIPLYIKTLVPEYKIFIRHYGYDVRDTICYAVI"
     gene            38403..39467
                     /locus_tag="PKU88_02950"
     CDS             38403..39467
                     /locus_tag="PKU88_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX68272.1"
                     /translation="MSKNPFVSVVINNYNYSEFISEAIESVLKQTYKNFELIIVDDGS
                     TDNSVSIIEQYHKQNQRIITPVYKKNGGQASAMNTGYNMAGGEIIALLDSDDYWFPEK
                     LERIVKCHENYDIVQHNLLINGEQKYINLRNDVDWSTALKKYGYLLGYWVPTSGLSFS
                     KALLGKVFPIPEVSLRLCADAYIIWNALYYSKIFSIDEALGFYRVHGNNNWHNKFNDE
                     RIKNTIDTILDILNEKLILSGESETIQYNSTLYKDLLGLFIEKDKKYVLYGTGSLSEE
                     FTRIVEQCESEIVYYSDSNSDKWGKHFMGREIIPPEDLLSKREHFDKIVIASSFIDEI
                     LIVLEKLGFVKASDIIVPQL"
     gene            39517..40857
                     /locus_tag="PKU88_02955"
     CDS             39517..40857
                     /locus_tag="PKU88_02955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPX68273.1"
                     /translation="MNICVIGTGYVGLVTGTCFAEMGNKVWCVDVDNAKIEMLNKGIS
                     PIYEPGLKEMIERNSKNGRLKFTTDIKEGIESSLYCFIAVGTPPGEDGSSDITNVLKV
                     AGAIGQNMNKYTIIINKSTVPVGTGEKTKELVREELRLRNELNIDFDVVSNPEFLKEG
                     AAIEDFMRPDRIIIGTDNPRTAELMKQLYEPFVRNQHPILIMDIKSAELTKYAANSML
                     AARISFMNEIARLCDITGADVNNVRVGMGMDSRIGMAFLYAGTGYGGSCFPKDIKELI
                     SLGSRNGLEMKIAKAVEEVNESQKHYLVNMVTKRFGCDLKDMSFGIWGLAFKPQTDDM
                     REAPSLVIMKELTGMGANIIAYDPEAIEQSKCIMSEDEYNIKYVYDMMEAIDKVDALI
                     LVTEWRQFRQPDFREMKSRMKQSIVFDGRNQYDTIAMQENGWEYYCIGRNTNVK"
     gene            40847..41788
                     /locus_tag="PKU88_02960"
     CDS             40847..41788
                     /locus_tag="PKU88_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPX68274.1"
                     /translation="MSNKRILVTGGAGFIGSHLCERLLKDGNHVICLDNFFTGSKRNI
                     AQLMNSKDFELITGDVENPIDIEADEIYNLACPASPVHYQYDKVKTLKTNVIGAMNML
                     ELAKKYDAKILQASTSEVYGDPMIDIQDEGYRGNVNPIGIRACYDEGKRCAETLFFDY
                     MRQYNVDIKVVRIFNTYGPKMHPEDGRVVSNFIVQALKGENISIYGDGSQTRSFCYVD
                     DLLEGLISMMNTSKDINGPINLGNPEEYNMLELAEKVIKLTGSSSKLIFKELPEDDPK
                     KRKPDISLAESVLGWKPSIAVEDGLIRTIGYFSELIR"
     gene            41847..43349
                     /locus_tag="PKU88_02965"
     CDS             41847..43349
                     /locus_tag="PKU88_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX68275.1"
                     /translation="MKKSIILFGASKSGEAAMKKYTKEYKIAYFCDNDKNKWGTSFRG
                     VEVVSPDNLKMLDFDKIIITSMYYDEISKQLSNMGIEKYEVFKLSAKERSNKSLREYI
                     TSMHCKIQYIYEVSRSAYWDEQQRKWKQKLDFNKMAEELSKQLRAAGLDKPVLYDVSI
                     KNDILKERPKIIHAIPNTFVGGSTQLIVDLIEHLGHKYDMEIITAALPKEGMHKGMTI
                     HDFSGAKSGEEVSEFLKSKNADLLHIHYWGDTWYEHIFDSLNTYDCKVVENINTPIAA
                     YAHNKVDEYVYVSEYARRMFGDFPENSSVIHPGIDLSHFEPSKDNKRPYAVGMVYRLE
                     QDKLREDSIKVFIELAKRKPELLIYIVGYGTFFNSYVRQVYKENVRENFVFAGYVPYK
                     ELPAIYDKFSIFVAPVWKESFGQVTPFAMNKEMVVTGFNIGALPEILDGEVTLGNSLE
                     ELVERTIYYLDNQEAYRAKGSELRRRAQDCFCVKAMVSKYEQIYDKLLSM"
     gene            43510..44424
                     /locus_tag="PKU88_02970"
     CDS             43510..44424
                     /locus_tag="PKU88_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPX68276.1"
                     /translation="MNILVIGGQGFIGHNLVLKLVSEGHKVTVFEKNFKPERKLDSCD
                     YTIGSFSEIAQYENVLEGMDIVYHLISTTIPKTSMDNPSFDIQSNVVDTIKLLELCCK
                     HKVRKIIFPSSGGTIYGLAETIPISEDHPTNPICSYGITKLSIEKYLHMFNHLYGLDY
                     QVFRISNPYGPFQNPFSNQGAVGVFLGKILRGEEIEIWGDGSVCRDYIFISDVVQALY
                     LASEMHTDIKVLNIGSGKGVTLNDLVDIISRVTSKEAKVVNKESREIDLPVNVLDISK
                     AADKLMWFPQIPLEKGIEITWDWLMRYK"
     gene            44424..45581
                     /locus_tag="PKU88_02975"
     CDS             44424..45581
                     /locus_tag="PKU88_02975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX68277.1"
                     /translation="MNILHITTFLQGGAGRIICDLAIEQKKLGHNVYVVTSKTQESDY
                     CNYAEYINRLNNADVILYEIDSTFKRDLYLNLKVVEKVKEIIINSDIDLIHAHAAIPA
                     LVGLISRQASHKYIPVIHSMQGWGTNKTPEQEETDKIIMNGLDAIIASSQSSFDLMCD
                     KGINKEKIGLIYNGIACTDISESDKVDDDVREMIKLKHEGVKTIGCIGTICKRKNQGL
                     LLEAFKEVVKAEDAICVFVGEGELVHELMQKANEYGIADRVRFWGYKENSYQFYKYFY
                     CVVLPSLSEGHPITTVECFREKVLFIGSDIPSITELIKHGETGFLFKSNNPASLAGVI
                     KQVLSLTDDESAVIAEKAYDFYSSSFTLNAMLDNYMRLYEALILKGKGLHK"
     gene            45614..46387
                     /locus_tag="PKU88_02980"
     CDS             45614..46387
                     /locus_tag="PKU88_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020756465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPX68278.1"
                     /translation="MKGIILAGGTGSRLYPLTKVTNKHLLPVGKHPMIDHLIAKIKQA
                     GICDIMVITGKEHMGAVVNLLGSGHDYGVKFTYRIQDQPGGIAQALGLCEEFVHDDRC
                     LVILGDNIFEDDISEYVENFMQQEKGAKILIKKVPDPQRYGVAELKGGNIVGIEEKPE
                     KPKSDCCVTGIYMYDNRVFNVIKSLKPSNRGELEITDVNNWYIKDGSLTFDVLEGWWT
                     DAGTFSSFAYANELAKDKDLSGILCRDYEILREAAITKE"
     gene            46408..47289
                     /gene="rfbD"
                     /locus_tag="PKU88_02985"
     CDS             46408..47289
                     /gene="rfbD"
                     /locus_tag="PKU88_02985"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPX68279.1"
                     /translation="MKMLITGAAGQLGSELLRIAKEMNCSLGAIDRSYNNPDIMALGA
                     KELDIADLPAVRNIVGRFRPDVIINTAAYTNVDGCEKEAEAAFKVNALGARNVAIAAQ
                     ETNSKLIHISTDYVFPGGGSTPYREYDKTSPKSIYGKTKLLGEEYVREFCKKYFIIRT
                     SWLYGRNGRNFVRTILSAAKEKQYLEVVDDQKGCPTNAEDLAYHILKIAVTDEYGVYH
                     CSGNGECSWYEFAIKILEIAGIDCPVKPIKTNQLDRAAERPAYSVMDNMMLRLTVGDD
                     MRSWEAAIEHFIKGLLI"
     gene            47286..48341
                     /gene="rfbB"
                     /locus_tag="PKU88_02990"
     CDS             47286..48341
                     /gene="rfbB"
                     /locus_tag="PKU88_02990"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPX68280.1"
                     /translation="MKTYLITGGAGFIGSNFIQYMLNKYSDIYIINYDKLTYAGNPEN
                     LKSIEKDSRYNFVQGDICDKEKLSELFDKHDIDYVVNFAAESHVDRSIKDPEIFIRTN
                     VLGTVNLLNGAKADWSEGQGFRRGKKFLQVSTDEVYGSLGDTGYFTENTPLNPHSPYS
                     SSKAGADLVVKAYYDTFGMPINITRCSNNYGPYQFPEKLIPLIINNCLNKKSLPVYGD
                     GLNVRDWLYVEDHCKAIDNVIKSGEPGEVYNIGGSNERTNIHIVKYIINYLHDNVDKE
                     IDEGLIKYVEDRKGHDRRYGIDATKIGEELGWAPETGFEDGIVKTIEWYLDNIEWMSN
                     ITSGEYRKYYSSMYENA"
     gene            48369..48938
                     /gene="rfbC"
                     /locus_tag="PKU88_02995"
     CDS             48369..48938
                     /gene="rfbC"
                     /locus_tag="PKU88_02995"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPX68281.1"
                     /translation="MGRFIFSKTHIRGLYIIEPAVFEDGRGYFMETYNYNDFKEAGLD
                     MIFVQDNQSSSGKGVLRGLHLQKERPQGKLVRVLHGEVFDAAVDLRAGSDTFGSCYGT
                     VLSGENRKQLYIPEGFAHGFLVLSDTAEFAYKCTDFYYPEYEKGIIWNDPDIAIDWPT
                     DKVEGIILSDKDKRLPLLKSILDSFEPKQ"
     gene            48953..49873
                     /locus_tag="PKU88_03000"
     CDS             48953..49873
                     /locus_tag="PKU88_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPX68282.1"
                     /translation="MKLLITAVGKRVQLIKYLKRQFTVIGADCSMLAPASFHVDRFCP
                     VSPCDNPGYIGEVLNICRKYGIDILLPLYEKEFYLLDSIREELLNEGTLLLLSDKRVL
                     DICSDKWETYQFFIGSNISTPISFKNKAECNIEFPMFIKPRRGMGSFNSYELNDMEEF
                     DFYYERIPEPIVQEKLSGVEYTMDCLSDLGGKVISVVPRERLEVRAGEVTKTRTVKDM
                     EIIEKTRYLLECLGSAGPTTVQCFRTVEGDIKFTEVNVRVGGGVPLTMEAGIDYGKIF
                     IDLIEGNKPEILLGNFKELTMLRYDEAVFI"
     gene            49870..50541
                     /locus_tag="PKU88_03005"
     CDS             49870..50541
                     /locus_tag="PKU88_03005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPX68283.1"
                     /translation="MKALVFDLDDTLYNEADFVNGAFMEVAQYLSKKHCIFSHELYHA
                     MLKLLQEKGRGRIFNDICDLYNLDEDINNLIKIYRNAVPPISLYEDAEYFLKYCQGKY
                     KTGLITDGLYYVQRNKIRLLGIEKYFDSIIVTDEHGEGFCKPDIKPYLKMAENLGVAL
                     DSMIYVGDNPRKDFCGARQLGVYTVRIIRPAGDHMGLRLGSDYEADREITNMYELKAV
                     IDTLK"
     gene            50558..51472
                     /locus_tag="PKU88_03010"
     CDS             50558..51472
                     /locus_tag="PKU88_03010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX68284.1"
                     /translation="MEHRYEKHWEHKEYKIIECMDCGFKHLYPVPEKESMKEFYDEKY
                     YSEIKPFYYKNVTEDFILRKSEEVDNNKIYGAIYDKVGEYKRAYENVMVDIGSGNDLL
                     ALYFQNKEWEVCVVEPNIKAAEYLNSYNLNVINDYMENIKPEILKGSSFINVQFVLEH
                     IHNPAAFLEKAYRMLVPGGIIRICVPNDFSEGQMAFKEYHSEAYRWIVLPDHINYFNI
                     NSLSGLLSRTGFRELYRTTDFPLEILLMGGMNYYTEEEYKKQVGPFIYNFENSLKSTG
                     RKQLLDRFYEGLANIGMGRSIYMYAIKE"
     gene            51492..52211
                     /locus_tag="PKU88_03015"
     CDS             51492..52211
                     /locus_tag="PKU88_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HPX68285.1"
                     /translation="MVTCIVQARMGSERLPGKVLRKVLGIPLILLNLKRLEKSREIDR
                     MILATSDRQLDDPLYETVKGEGFEVFRGDEENVLKRYRDCVLEYGGDIVVRVTGDCPL
                     IDPDIADNVISHYKMYDYDYVRLDVPDTFVRGFDTEVFSREALFKAFDLADEYRYKEH
                     VTAYMYLHPELFRTGLVRGESLFKKNYRLCVDTLEDFTLVEKIYEHFGDIYVSAGDVV
                     KFLDNNPDIAAINSEVVQKHV"
     gene            52223..53257
                     /gene="pseI"
                     /locus_tag="PKU88_03020"
     CDS             52223..53257
                     /gene="pseI"
                     /locus_tag="PKU88_03020"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HPX68286.1"
                     /translation="MNEIVLKNGRRIGEDNPCYIMTDIAANHNGDLETAKELIRKAAE
                     AGADAVKFQTYRAEDLYSKKTPQFSRDSMKPYDLIKKVQHPREWIPILSEHAKGFGID
                     FLSSPFDYDAVDLLDNTGVSLFKVASLEIVDLKLIKYIAGKGKPVVLSTGMASLGEIE
                     EAIEAARSTGNGDIALLHCNTCYPAPAHIVNLKAMETLRNTFRLPTGFSDHTLGWHIP
                     VAAVALGACIIEKHFTLSREQEGPDHGFSIEPEELKLMVRQIRDVEKAMGDGVKKVSR
                     EELENYEKGRRSLIAITDIPKGTKITESMITAKRPGYGIKPKYFDMVIGRTAKVDIFE
                     DDVLLWDMLV"
     gene            53282..54274
                     /gene="pseG"
                     /locus_tag="PKU88_03025"
     CDS             53282..54274
                     /gene="pseG"
                     /locus_tag="PKU88_03025"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HPX68287.1"
                     /translation="MKIVIRAEGGPGIGMGHVMRMLVLADKLKAFAEVVFACRDNKEF
                     EPGVRQIEACGYSVWRFSGETVIDGLAAIGGDCLITDSYDIDEGYFDRTKDIFGITGY
                     MDDINKVRIYADFIINQNIYAEDMEYKAGIGTKLFLGTRYLLLRDEFRNLPKRKTRMH
                     IEDVLITLGGADPGNLSETIALKLSKVFPEITFHIAVGPSFTNRDSIERIAGGNIILH
                     YNPKMSELMLKCDAAISACGSTVYELCACGTPIIGVAAAPNQIMTSRSMDIKGALKYA
                     AGAGEIVQQLESLNYNTRARMSDTARELVDGYGSIRVSEQIWKIICEKEKGGTV"
     gene            54271..55416
                     /gene="pseC"
                     /locus_tag="PKU88_03030"
     CDS             54271..55416
                     /gene="pseC"
                     /locus_tag="PKU88_03030"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HPX68288.1"
                     /translation="MRKFIPYGRQYVDEDDIQAVVEVLRSDFLTTGPKVDELENKITE
                     YTGAKYAAVLSNGTAALHAACFAAGIGPGDEVITSPMTFAATANCILYMGGKPVFADI
                     DPMTYNIDPWDIEKKITHRTKAIIPVHFTGQPCEMDEIIRIADNHGLTVIEDGAHAMG
                     AEYKGRKIGTIGTMTTFSFHPVKHITTGEGGAITTGDASLYEKLTLFRTHGITKNAEK
                     MTDYDGLWFCEQQYLGFNYRMTDIQAALGVSQMKKLDSFLEKRRSYAALYNKLLGTID
                     GLTIPYQNEYASSAWHLYIIKLNLSKLDIGRREVFDKLRDRNIGVQVHYIPVYYHPYY
                     KNMGYEKGLCPEAEALYEGIITLPLYPTMEIEDIYYIADNLRDILRS"
     gene            55423..56370
                     /locus_tag="PKU88_03035"
     CDS             55423..56370
                     /locus_tag="PKU88_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX68289.1"
                     /translation="MNMLVTGGAGFIGRWTVKRLLEGGHKVWILDDLSNGSLDNIKEF
                     EGHKALMGFLKGDIKDNSILERLFENNFDICFHLAASINVQDSIDDPRTTFENDVAGT
                     FNVLEQCRKHNVKMVFMSTCMVYDMAENDQGINELHPVKPASPYAGSKIAGESLALSY
                     WHAYKLPVVVVRPFNTYGPFQKSGGEGGVVSIFIERKLKGQALNIYGDGKQTRDLLYV
                     EDCAEFLVMAGCSEKSNGEIINAGSGKDLSINTLAYMISGNYEQVRHVTHIHPQSEIQ
                     RLLCNYEKAEKLLNWSPRTGLQEGIEKTEAWLENNTIKL"
     gene            56462..56845
                     /locus_tag="PKU88_03040"
     CDS             56462..56845
                     /locus_tag="PKU88_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HPX68290.1"
                     /translation="MKIAGNSISPAVSSDTMRNNAVKGNHAEISNRNNAVKSGEQEVS
                     AKPKDKSEINEYQFIRAVETANKALEGSFTSFEISVHEKTNRIMVKVVDKETKEVIRE
                     IPPEKILNLMAKLWEMAGIIVDERR"
     gene            56851..58791
                     /gene="fliD"
                     /locus_tag="PKU88_03045"
     CDS             56851..58791
                     /gene="fliD"
                     /locus_tag="PKU88_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPX68291.1"
                     /translation="MASNINSVSTLRFSGIASGLDVDDMVEQLMKVERSKMDKVKQDK
                     QLLEWKRDDYRSITSLMRSFQSSYFDVISSTNMRSSTSYKAFSTTVSPSDAADAGAVS
                     AVATSAASEGTHSIIVGNLATAQVRQSAGGVTKDVQGDSGYTLTAGETFKLTIDGVTK
                     SITLSDLDGTDGVTLADLNKSIENAFGSGKVTVDDTTNPGKLTFKVSSETNGINKITV
                     SAGTTNNALANMGFGADAIYSNRLSSGDTLAVLKSKLDDSNGGLAFTALSDGTEGIKL
                     AINGKTFEFSETTTLYSMMNQINRDSTANVNMQYDEINDKFKFTAKQTGAGNNIEISE
                     SGSSFITTADISVNNAGEDAKITIDGTEITRSSNTFTADGITYTALKETGTREVTVST
                     KQDVDTVYNKIKSFVDKYNELISKINGELTEERDRDYAPLTDDQKEDMTEDEIKRWEE
                     KAKSGTLRGDSLLQKIVSDLRNTLYAGVEGESGTSFLFSIGIETGSWNNKGKLVINES
                     KLKAAIKDSPDKVAEIFSKESSIAYSADMTSTERSTRFKENGIINRMYDVIQDNIRTY
                     SNKNGQKGALLEKAGVVGETTENKNIMYSMIKTQSDMITTLTKKLQTREDYYYKQFTA
                     MEKAISQMNIQSVWLSQQYDSQ"
     gene            58811..59206
                     /gene="fliS"
                     /locus_tag="PKU88_03050"
     CDS             58811..59206
                     /gene="fliS"
                     /locus_tag="PKU88_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPX68292.1"
                     /translation="MAMANNAHDIYKKNMVTTATPQELTLMLYNGLIRFLKQAVMGID
                     EKSIEKANNNIIKAQDILFEFMRTLDMSYEISNNLLVLYDYMNTRLVEANISKDKAIV
                     EEVIGYAEELRDTWAQAMKLAKQQAVVNR"
     gene            59274..59756
                     /locus_tag="PKU88_03055"
     CDS             59274..59756
                     /locus_tag="PKU88_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68293.1"
                     /translation="MDNTNSVIIELLSLTDKKKQLFDSILEITLAQKKDIEENAANKI
                     EGLVSKKQSVIDSIDEIDKSFSEKFKLLKKQLMVNSLEEVDFTKYPELKNLKLKVEEI
                     MSMAQEIMLIERSNKEKLVSIMNEMKREIKQIKAGKKSIKAYERPITNNDGIYVDKKK
                     "
     gene            60168..60455
                     /locus_tag="PKU88_03060"
     CDS             60168..60455
                     /locus_tag="PKU88_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HPX68294.1"
                     /translation="MFEDKTLICKDCGNEFTFTAGEQEFYAEKGFQNEPARCKDCRMA
                     RKNASGRNGQRREVVMYDTVCADCGKETQVPFKPTNARPVYCKECFDAKRQ"
     assembly_gap    60882..60981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            61096..61626
                     /gene="raiA"
                     /locus_tag="PKU88_03065"
     CDS             61096..61626
                     /gene="raiA"
                     /locus_tag="PKU88_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPX68295.1"
                     /translation="MRTTVSGKNIVITNALKERVEKKLSKFERYFGPDTEVQATLSVE
                     KNRHIIEVTIPFNGVILRGEEATGDMYTSIDNVVEKLERQMRRQKTKLEKRIKDGNIK
                     FENWAAPPAEFEDEEVKIVRTKRFAMKPMPVEEAVLQMELLGHSFFVFSNADTEEVNV
                     VYKRKDGNYGLIEPEF"
     gene            61852..62484
                     /locus_tag="PKU88_03070"
     CDS             61852..62484
                     /locus_tag="PKU88_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68296.1"
                     /translation="MSNNKAILYNDIQSLICKLPDIINCSVICDKEGKIEEIHILAGA
                     GRNIKQMVRDIQSAISAKFNISVDYKKISIAQINEAEFKETRVRIEGIQVKNVNNAIE
                     AEVTLSYDDKVYQGRSSKVKSRSNRAKAVAEATLAALEACIGISGVLYLEGLENFKVS
                     DKEVFVSLVGFSKDNIDEYYSGSSIISLDENESAVKAVLAAVNRKVNTVA"
     gene            62700..63977
                     /locus_tag="PKU88_03075"
     CDS             62700..63977
                     /locus_tag="PKU88_03075"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPX68297.1"
                     /translation="MPIKLKAYVAAIVLLGTIFFVFTLNQAHEVDAVGLVIFTLLSVI
                     AESMVIVTQGQRALSVGFIIGLAAIVVFGVPEAAWITSIGVMLRVVRHEGKTYHPFNF
                     PIEKTLFNGANATISAGLAGLCYEALGAVPGTIDLNSSIAALIAVITAMVIYILVNAV
                     IMSILMHLITGEKFFSNFINNMAWVVKDYLAMAPFAVIMAMAYANYKVAGILIFFIPL
                     LFARYSFKMYIDMRSIYIDTVKSLSQAIEAKDPYTNGHSRRVGEYACKLAERLGLPPK
                     KVENLKIAAILHDIGKIGIDESILNKPGRLTTEEFDKIKQHPEIGVKIIKDIDFLKDA
                     SEIILSHHERYDGTGYPEGRKYKDIILEAQILSIADVFDALTSERPYRNAMTVEEALD
                     IIEKGKGNQFESRLADTFIRMIKDDKEMKKVAG"
     gene            63967..64521
                     /locus_tag="PKU88_03080"
     CDS             63967..64521
                     /locus_tag="PKU88_03080"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HPX68298.1"
                     /translation="MLVESVGTSLAVGKMRGGSFSNIKDASLEKWYFFVLGFMVEFAA
                     VYAASRNIGFFKDNILIVHFLSYLLLFIGIYFNRRTLSFRIVFIGVFLNFLVIMANGG
                     QMPVSAEAMADIGLIDNMIAIRDGEIITHTLMNGNTALKYLGDILVLAKPYPRPKIFS
                     IGDVFMALGIFMYIQEIMVKKAAK"
     gene            64753..65064
                     /locus_tag="PKU88_03085"
     CDS             64753..65064
                     /locus_tag="PKU88_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68299.1"
                     /translation="MPHNSYNNIKKYAAFIFMVFTVFSGSSEVFDSDINTFAVSRFVA
                     QNARLSLSRTHIQSSVQNNESLRKYPDAENIQACVSFTILICILNNIIMKNSIWKNAL
                     F"
     gene            65122..67620
                     /gene="secA"
                     /locus_tag="PKU88_03090"
     CDS             65122..67620
                     /gene="secA"
                     /locus_tag="PKU88_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPX68300.1"
                     /translation="MFLDKVFGNPNDREIKKLMKTVQEINKLEPAMQKLSNEQLQSKT
                     DEFKKRLADGETLDDLLVEAYAVVREASVRVLGMRHFDVQMMGGIVLHQGRIAEMKTG
                     EGKTLVATLPLYLNALTGRGTHLVTVNDYLATYQSQWMGKLYNFLGMSVGLIVHGLEP
                     EDRQKAYRADITYGTNNEYGFDYLRDNMVIYRDQMVQRELNYAIVDEVDSILIDEART
                     PLIISGVGEKSTQLYYVADTFVRGLKNEADFTIDEKAHTVVLTEEGVAKAERFFNLEN
                     LADANNMEIQHHINQALKARNLMRRDIDYVIKDGQIIIVDEFTGRLMFGRRYSDGLHQ
                     AIEAKEGVKVERESQTLATITFQNYFRMYGKLAGMTGTAKTEENEFLAIYNLDVVEIP
                     TNMPMIREDYPDAVYKNKRGKFNAVVEEIVRLHEKGQPVLVGTISVETSEMLSDMLKK
                     RGVPHEVLNAKHHEKEAQIVAQAGQLGMVTISTNMAGRGTDIVLGEGVKELGGLHILG
                     TERHESRRIDNQLRGRAGRQGDQGSSRFFISLEDDLMRLFGSEKIMKIVESLGMEEDM
                     TIEHPLLSKSIENAQKRVEGRNFDIRKHVLQYDNVMNKQREVIYSQRRQVLLGEDIRE
                     NIMEMIAEIIGDAVATYTIGSEFAEEWDIKGLMEYLHTIFLPKNAFAREELLNFTREE
                     LTEAITGKAVDIYEQKESQVDPQVMRELERVALLRSVDTKWIDHIDAMDQLREGISLR
                     AYGQKDPVQAYQIEGYDMFLELVKNIKEDTVRFIYHVNISNLPQREKVAEPVATNQEG
                     GVRKPVVKEDKIGRNDPCPCGSGKKYKKCCGMNK"
     gene            67634..68741
                     /gene="prfB"
                     /locus_tag="PKU88_03095"
     CDS             join(67634..67708,67710..68741)
                     /gene="prfB"
                     /locus_tag="PKU88_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106406468.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPX68301.1"
                     /translation="MLELEQYKYGLSEIWDAIEEIEVSLDLTSIQERIYELEQEMNQE
                     GFWNDAENAGKVLKTIKALKDKTEKIENLKKEYEDIEVLIELGMEAQDESVIPEVEKS
                     FKTLQKDVESFRIETLLKGQYDRNNAIFTIHSGAGGLEAQDWAEMLLRMYTRWAERKG
                     YAITILDMLRDYEAGIKSAALLIEGENAYGYLKSEKGVHRLVRISPFDTAGKRHTSFA
                     SVDVMPELDDEVKVEIDEEDLKIDTYRSSGAGGQHVNKTDSAVRITHIPTGIIVQCQN
                     ERSQHSNKEMAMKMLFAKLLEIKEQEHKDKIDDIKGEYREIAWGSQIRSYVFHPYNLV
                     KDHRSGEETGNTGAVMDGELDDFINAYLKQGNGV"
     gene            complement(68744..69184)
                     /locus_tag="PKU88_03100"
     CDS             complement(68744..69184)
                     /locus_tag="PKU88_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPX68302.1"
                     /translation="MVNKKMFIVDSDILPKAYEKVIEAKLLLRTGDAATIQEACRMAG
                     VSRSAFYKYKDSVFDYTAQERGRTVTLFMILKHIPGVLSDIINKIASVKGNIITINQN
                     IPVMDSASLTASIDTKIMEINPEELVELLLSCKGCKKVEIIGME"
     gene            69330..71456
                     /locus_tag="PKU88_03105"
     CDS             69330..71456
                     /locus_tag="PKU88_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPX68303.1"
                     /translation="MDIIAKLAKELSVKPWQVENTVKLIDDGNTIPFIARYRKELTGE
                     LSDTTLRELGERLTYLRNLENRKEEVLRLIEGQGKLTGELKASIEKAEVLQEVEDIYR
                     PYKQKKRTRATIAREKGLEPLADIIFSQERQSKALEEIAEAFVNDELGVSTVQDAING
                     ALDIIAEIISDNAEFRKEIRELTFGNGTIVVKGTKEEKSEYQMYYDFSEPVKKIAPHR
                     ILAINRGEKEEFLNVKAEAPADRILTYLNNSTIKENSPYKNMLEETVRDAYSRLIAPS
                     IEREIRNNLTEEAEEQAIKVFAANLRNLLLQAPIKGKVVMGFDPAYRTGCKIAVVDDT
                     GKLLETAAVYPTPPQNQAEEAKMILKKLIKKHNVEVISLGNGTASRESEKICAEIIKE
                     MNMPLHYVIVNEAGASVYSASELASQEYPDINVSLRGAISIARRLQDPLAELVKIDPK
                     SIGVGQYQHDVAQKKLSDSLNGVVEDCVNSVGVDLNTASPSLLQHISGINSAIAKNIV
                     SYREENGKFRSRRELLKVKKLGEKAFEQAAGFLRIPESENILDNTSVHPESYDVAGKL
                     LEKAGRDISAAVDKLSAVDVNELAAELGVGVPTLRDIINELKKPGRDPRDEMPKPVLM
                     SDVLDIKDLKPGMILTGTVRNVIDFGAFVDIGVHQDGLVHISELADRFVRRPLDVVQV
                     GDIVKVKVLDVDLDRNRISLSMKGLN"
     gene            71522..71998
                     /gene="tsaE"
                     /locus_tag="PKU88_03110"
     CDS             71522..71998
                     /gene="tsaE"
                     /locus_tag="PKU88_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPX68304.1"
                     /translation="MLKFHTESAEETAKIGEGLGRLLSPGDIVCLSGDLGAGKTAFTQ
                     GIARGLEVKDYVTSPTYTIINEYEGRVPLYHFDVYRLNDVEEMYELGYEEYFFGDGVV
                     VLEWADMVSDIIPEDRLWITILNTKGDNSREIIMEPAGEAYEAIVKGMESNENTGC"
     gene            71979..>73006
                     /gene="tsaB"
                     /locus_tag="PKU88_03115"
     CDS             71979..>73006
                     /gene="tsaB"
                     /locus_tag="PKU88_03115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPX68305.1"
                     /translation="MKILAVDTSTSAATAAIMEDDRLISEYILNISRAHSRKIMGIIE
                     ELFNKSGIKPAEIDIYACSAGPGSFTGLRIGASIIKAMAQTFDKPVVGVPTLDALACN
                     LYNCTGLVCPMLDAQRGMVYSSLYSYNSGRLTKLEDLGVIPIEDLIELVKSKAVPTTF
                     LGDGAVIFEENLKKGLEGYNAAPAGSLLPRASSCARMALEMYNKGSFDTYNSFRLTYI
                     RKSQAEAEYEKKNKAEFQSMSLDDIDQVVEIENLSFASPWSRESFESELLKNKLARYI
                     VAKVNGKVAAYGGMWIILDEAHITNIAVHPEFRERKIGEKLVMEMLRTAKENKAEHIT
                     LEVRASNDA"
     assembly_gap    73007..73106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <73107..73577
                     /locus_tag="PKU88_03120"
     CDS             <73107..73577
                     /locus_tag="PKU88_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPX68306.1"
                     /translation="FRMMGNREEAEDVSQEAFIRMYNSLSKYNPEYKFITWALKVTTN
                     LCLDSLRKRKAETVPIDDVFEIKDGKDTPEEEYIRKENQKLVQDAIMKLPDKYREFLI
                     LFHHRNLSYQEIMDITGESLTIVKNRLYRARQMLKEELISREKEREKYAVPGNK"
     gene            73555..74112
                     /locus_tag="PKU88_03125"
     CDS             73555..74112
                     /locus_tag="PKU88_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68307.1"
                     /translation="MQCQEINNLVMKYFDGNISELEREMIIKHNEKCNRCAEEFGILR
                     NAIDTLEELPEIEVPAGFESRVMEGILSRRAYSTNPKIVAFWLLSVLGLMVFGWNMAA
                     YVIMPLIRESGIIIAANNVLIYGFNVVSGILREVVVTVSKLLGKILVMRNVLLRDYIT
                     SVTLIVLAFMAINVFLAYRRKPQEN"
     gene            74124..>74464
                     /locus_tag="PKU88_03130"
     CDS             74124..>74464
                     /locus_tag="PKU88_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68308.1"
                     /translation="MKVKKRAALLAGLMLMAVMLCAFEINVDNKDKVAIFKDIEIPEG
                     VTVNGDVVALFGDVAVSGDLTGDAVTIFGDMSVSGAIEGDVVAVFGEISVKDGGIISG
                     DAVGILGGVDKS"
BASE COUNT        24634 a        11894 c        18105 g        19531 t
ORIGIN      
        1 atgggcgaag cgcaataatc gagggctgtg aaaagttcag cggagcaaag gtcagagcta
       61 ccgacctgag agcgggggcg gcattggtca ttgccggact ctgtgcggag ggaagaacgg
      121 aaatacatga tgtatatcat ttggacagag ggtatataag gattgaggaa aaattgaaaa
      181 agttgggtgc ggatataaga agagaagagg aataataact cccggtgcag cagcaccggg
      241 tttcttattg tatagaaagg cataactttc ttcaatcacc tttgttaatt tcacctatca
      301 tataatggcc tccttaaaaa taaatataga tatggcacat aagcaggaaa aagcgacgta
      361 gtcttcagac gcgaggcact agattttggg ttgcgaggct gcgaggttac gaggaaagcc
      421 agggtattcc ttatgggtaa agtaacgtat aacactgaat acacggcggg gaaatattct
      481 attactcgag taataatcga taacgcgtaa ggtatcggta ttttcccatt tagcgattat
      541 aacgataaaa atataataat taactgtgtt attccgtgaa acagccgtga ctcccgtgtt
      601 actcgtttct tgtgttgcat atggatatga atattttgga ggggggtacg attattgaag
      661 aagttggccg cagccattgc agtactaatg atattgctgt ttgccattcc cttgctcctt
      721 gtgagagaca agggagaaga gtcggggaaa aaggaagtgc cccgggagga tctgcccata
      781 gaggtctata tgtgtgatac agatgaggtt gtgacaatga atctggagga ctatatatta
      841 ggtgttgttg caggagaaat gcctgcgagc tttcatcctg aagcccttaa agcccaggca
      901 ctggctgcaa ggacttatac aatgctcagg atgaggcagt ttggaggcag cggctgcagc
      961 aagcaccagg gagcggagat atgcacagac tccactcact gccaggcata cagggatccg
     1021 gcgtcagtga agaaggacct ggataagctg aaggatgcgg tatatgggac agcgggagag
     1081 gtaatagtct atgataataa gcttatcgat gcggttttcc actccaccag cggggggaaa
     1141 acggaaaatt cagaggatat atggtcaaac agggttcctt atttgcgaag cgttgtaagc
     1201 caatacgagg aacactcccc caagtatgtg gcccggcagg agataagcat tgataatttt
     1261 gtgcagtgta ttaagaagct tgactcaggg gtggtaataa ataaaaagga tataaagaat
     1321 gaaataaagg tgctggagag gagcgaggga ggaagaataa cccgtataaa gatcggaaac
     1381 aagaccttta agggtacgga tgtgagaaat acactgggtt tgaactcctc aaacttcaat
     1441 tttgatgtga ccgcaagcac tgttctgttt actgtggtag gaaacggcca cggaataggc
     1501 atgagccagt acggtgccga cggcatggca aaaaacgggg catcatacag ggatattgta
     1561 acccattatt accaaggggt tgcaatagtg agtataaagg atttaacaaa ttaaaaattt
     1621 cgccgaatgc ctgtattatt tttaaaatta ttggaaataa tactggcgga ggtgtaaatt
     1681 atgaaattta gaaaggaatt caataatagg catatatatt ttcttgtctt gttgatagct
     1741 gcattgctta ctgcaggagg agtaatgcag tacagaaact accttttgga taaacaggca
     1801 caattggact ttgagaaaag catggaggaa tacattgagg agtctgaacc cagttctaca
     1861 gcgcaagtaa tcgaggaaaa ggcggaaagc ggcggaagtg aaaatgttgt agaaacaccg
     1921 gatgcaacag aagtatcaaa cagcagttca gtaaagataa ccgaagataa aaaggatacc
     1981 aaagagaaag tcaatgaagc caagcctatt tctgcaataa aaatggaaga gccggcaatg
     2041 gagacaatgg tggttcctgt atttgggact atgtatacgg aattttcaga ggatgtgctt
     2101 gtttactcaa aaacactgga tcagtggagt acacatgcag gaatcgatat aaaggcggaa
     2161 gagggaagcc ctgtaagagc agcgatggat ggtgtggtgg atcaggttgt aaatgatccc
     2221 cagcttggat taaccattgt actggatcac ggcggcgaag tctacactaa atacagcaac
     2281 ctttctaccc tcgatttggt aagcataaac cagagtgtca aaaaaggcga tgtgatcagc
     2341 ggtgtaggga aaaccgccct gtatgaaatt gccgacgatc cccatctgca ctttgaagta
     2401 atcaagcaag gcaagagtgt tgatccaaag aagtacctgc ccaaacaagc actaaagaaa
     2461 tagtgcggcc gggcatcaaa aagcattaat taatgaacga tacgtgtggt tctaattacc
     2521 aaaaaaaccg catattaatg taaaaagaca ccaataaggg ggtagtagta attgaaggat
     2581 tatatcgaag aaagagcatt ggaaatagcg aaatatataa taagcagcaa gacaacggtt
     2641 agagatgcag ccaaaatatt cggggtaagt aaaagcacag tacataaaga tgtcacagaa
     2701 agacttccga gaataaatcc cagtgtagca cttcaagtta aggagataat ggaccagaac
     2761 aaagcagaaa gacatataag aggcggaagg gcaaccaaga tgaaatatca gtccataagt
     2821 ttgtagtgat gaaggttcag gctccggcaa gtattgatgt gctgatgctg ctgcattgca
     2881 cattatattt gccggagctt gttatttggg gttatttgtc ttataattgc agtagacaag
     2941 ccataaaata gtcactactt ttttgttata ataatgtact tcgtgaagga attttgtttt
     3001 ttatatagaa atataaaagg attgataatg acaagcgaaa tattatggag gtattaaaat
     3061 gtggggagga caagacatag gcatcgacat gggaacagcc agcgtgttgg tatacattaa
     3121 aggaaagggc atagttctga gagaaccgtc ggttgttgct atagacagga atacaaacaa
     3181 aatattggct gtaggtgaag aggcaaggct tatgcttggc aggacacccg gtaatattgt
     3241 tgcaatcagg ccgctcaggg agggcgttat ttctgattat gacataactg agaaaatgct
     3301 cagatatttc atagacaagg ctatcggcag gagaaggata attaagccaa ggatggttgt
     3361 ttgtgtgccc agcggtgtta ctgaggttga aaagagagcg gttattgatg ctgccaataa
     3421 cgcaggggca agaaccacaa ggcttattga agagccaatc gcatctgcta taggagcagg
     3481 tttggatata tccaaagcca gcggcaatat ggtattagat attggcggag ggacaacaga
     3541 catagcggtt atatccctgg gaggaattgt tgtatcaacc tccattaaga ttgcagggga
     3601 caaatttgac gaagccattg taaggtatat gagaaagaag cacaatatta tgattggtga
     3661 gagaactgcc gaagagctga agataaacgc cggtacagcg ttcccgagag aagagcagct
     3721 taaggttgaa gtcaggggca gaaatcttat ttcaggactt ccaaagacca tagaagttac
     3781 ttctgatgaa atgcttgaag cgctttccga accggtttcc gccattgccg acgcggtaca
     3841 ttcggtgctg gaaaggacgc cgccggagct ttctgccgat ataagcgaca gaggcattgt
     3901 tatggcaggg ggaggttccc tgttaaacgg ccttgacaag ctgataagca aaagaaccgg
     3961 catacccgta tatgtggcgg aagacgcaat atcctgcgta gctattggaa cagggcaggc
     4021 tttggagcat atagacatgc ttcaggattc ggacataaat gcaaacagaa aaattatcac
     4081 attgaattat taagtcagat acaagagaag ggacacgggg acgtttctcc tgtcccgctt
     4141 ttagcgggac aggagaaacg tccccatgtc ccttcgaaac gtccccgttt ttttatctgc
     4201 taaagtcctt taaacattat accgataact ttcatgtaaa gaataacatt taatgtaggt
     4261 gatagtgtga tcagaggact ttatatttca gcctcctcag caattactga agcaaaaaga
     4321 gtggatgtaa tagccaacaa tattgcaaat gtgaatacta caggatataa gaaggatgtc
     4381 atggtgacac agaacttttc cgaggttctg atttccagaa taaatgacag gtttggcaag
     4441 gacttgaagg tttcaaggcc ctttaaacca ttggggcctt taagctatgg aatacacagc
     4501 agtgaaataa tgattgattt taaacagggg cagctgaact ctaccggaaa cagctttgat
     4561 ttagctttaa gagggaaagg gtttttctgt attgataccc ctgagggtga aaggtataca
     4621 agaaacggca gctttgcaaa ggatgcggaa ggctggctgg taaccgggga aggatggagg
     4681 gtctttggcg aagacggata cataaggata gaaggcagcg atatgacagt aaatgaaaag
     4741 ggcgaggttt tttccgatgc acagctggca ggcaagctca ggcttgtgga ctttgaagat
     4801 tatgaggcgc tgcgcaagga aggaaacggc ctcatcagaa tagcagaaga aattgaggcg
     4861 gaaccggtaa taacggcggg aacagtacag cagggttttc ttgagggttc caatgtcaat
     4921 tccgtaaagg aaatggttga aatgctgact atgttgagaa cctatgaggc taatcaaagg
     4981 ataataaaga tacatgatga actgattgga aaagctgtaa atgaaattgc gagggtttag
     5041 gaggtaataa actatgatga gagctctttg gacggccagt acggggatgt tggcccaaca
     5101 gcttaatgtt gataccatat ccaacaacat ggctaatgtc aattcagttt catataaaaa
     5161 gtccagagtg gaattcaagg acctgcttta tgaaacattg gaaagaggaa tagtaaggga
     5221 cggacagggg agacctgtca atctgcaggt ggggcacggt gtcatgcccg ccgcttcagt
     5281 caaattcttc actacgggaa acctggcttc cactgaaaac ccgtacgatg tggcaattga
     5341 aggtttgggc ttttttaagg tgctggggcc tgaaggacag gagtattata ccaaggatgg
     5401 agctttcaag atatcgccgg atggggagaa cttcaacctg gtaacctcag aaggctatta
     5461 tgttcaagga gagaacggca atatagaatt gggttcggat atcaaggata taataataga
     5521 cgcaagagga ttgataactg ttaaaagaga ggatggcacc attgaagagc tggggagcct
     5581 ttcaatatca acctttgtaa atcctgcagg gcttgagagt gtggggaaga acctttatgt
     5641 agcaacagta gccagcggag aagccttcga tacccagaac gacggcaccg gagggaatat
     5701 cctgcagggt tacctggagt actcaaatgt gcagattgtt gaggaaatgg taaatctcat
     5761 acaggcccag agggcatatg aaatgaattc caagtccata cagacagcgg acgagatgct
     5821 gcagatggca aataacctta ggagataatt atgattaatc ctattaataa taatggaata
     5881 acagattata ataatgctaa aagcaaagct caggaagcaa agcagggaga gtttgggaaa
     5941 gctcttgaaa aagcaatgga ggaaaaggac gagaagaagc ttaagcaagc ttgcagcgac
     6001 ctggaggcta tatttgtcag catggtcttt aagcaaatgc ggagtacggt gcagaaagcc
     6061 ggtttaattg acggcggtat ggcagaggaa atgtatgagg aaatgctgta tgacaaatat
     6121 gcggaggaag cttccaaggg cgaaggaata gggctggcgg atctgcttta tcagcagctg
     6181 tcaaaaagcc tgaagccgga aagagagagg gaagatgttg aataatacgg atattcaagg
     6241 aattttaccc cacaggtatc catttttgct tgtggataaa ataaccgaaa tagaatatgg
     6301 aaaaaaagcg gtaggtatca aaaatgttac tgttaatgaa cctttttttc aggggcactt
     6361 cccggggctt ccgataatgc cgggagtgct tatagtggaa gctatggcac aggtaggagc
     6421 ggtagcaatt ctgggaatgg aagagaacaa gaacaagctg gcggttttta cggggattga
     6481 cggaatgagg ttcaggcgcc aggttgttcc cggagataca ttaagaatgg aagtagaaat
     6541 gcttaatttt aaaaggggca taggcaaggc aaaagcccag gcttatgtgg gcgatgagct
     6601 tgcagcttcc ggagaactta tgtttgcgat agtcaacaaa taaaacggtg atgtattcac
     6661 cgttttttta tttggacaag tagggacgcc atatatggcg tccctactgc cgaaaggcgg
     6721 catatattcc ttttgttata gtttctgaca ttttcattac cagtcccagc cttacattgc
     6781 ttattttgag gtagttctca tattcactgt cacctgattc cacagcttca tcaactactc
     6841 caattataga aaagtctccc acatgggaga gccttttacc gacgcctttt ccgggctgga
     6901 tgctgccttc cttgacatga acatatccta cgcttttgcc cgcacccagg caagcgtcta
     6961 tggcgatgat tttatgatct ttataggagg tttttatatg agacatgacc cttctgagat
     7021 taaccgcatg aataggatta tccaaggtcc ccaggattgg gaccggaagg ctcagcttgc
     7081 tcagcaatgt gccggtcagc ggcccgagag aatctcctat gaacctgtcg gtgcctatac
     7141 aaagtattaa gcatttatct aatttatttg atttcaaaaa atcagagatt ttcaaatgtg
     7201 cccgattatc cgtatgatgc acacaaattc cgtcttttat gctgtatttc tcaatcaaaa
     7261 tatgacctcc aaacatttgt tttttgctct tcgttggatc ttgttataaa tatattggaa
     7321 gttcaggtca attattcata tcataatatt gagatgaatg agttgtgtaa gatgaaagcc
     7381 gtgatgtccc atattcctgg atgatttgtc tattgatttt ttagaatgct gaaattataa
     7441 ttatgaataa taagtatcat tcctcagcat attacaagat gagggatata atcttggatt
     7501 ttagttgttt tcggtcaaat ttgataattg attgatattc ttatttttta tataatagag
     7561 aagtcgcgtc ggggtgtagc gcagatggta gcgcacgtgg tttgggacca tgaggccgca
     7621 ggttcaagtc ctgtcacccc gaccatgtgt gcaagatatg gcccattggt caagcggtca
     7681 agacatcgcc ctttcacggc ggtaacgggg gttcgattcc cccatgggtc accaactttt
     7741 tccgcttcac ggaaaaagac cgacattcct tagtgtaatg cgcagcattt gcggagcagc
     7801 tttaaccaaa ctgtttagca aaattaaaaa gaccgacatt cattgattaa agccggagca
     7861 taagcggagg caaattatgc tggtgtagct caattggcag agcagctgat ttgtaatcag
     7921 caggttgcgg gttcgagtcc catcaccagc tccataatgg ggaaattccc gagaggccaa
     7981 agggggcaga ctgtaaatct gttgtcgcag acttcggtgg ttcgaatcca cctttcccca
     8041 ccattttaaa acaatagtcc gaaagggctt ttttcttttt ttgcgggaca cggggacgtt
     8101 tctcccggga cacggggacg tttctcctgt cccattggtg acaatttcct aaaaaatggt
     8161 tgcaatacga acatatgttt ggtataatta ggaaagaagg cagggggacg tttaagctgt
     8221 cttccgaatg atgaacttgg gagggtttga tatggcaaga caggccagga aaagaagcgc
     8281 aacaggtgtt tatcatgtca tgctaagggg aattgataag agagatattt ttctgcatga
     8341 cgaagataag atgattttta tgaaaaaact tctaaaagca aaagaaaatg caaaatttgg
     8401 cctatacgga tattgtttga tggataacca tatacatctt ctgatactcg aaagtgaaga
     8461 aataggagtt agcatcaagc gtgtatccgt aggctatgca tcgtggcaca ataatagata
     8521 cggaagagtg gggcatctgt ttcaaaacag gtatttaagc gaaccggtcg aatctgaagg
     8581 ctatttggtc catgtactga ggtacataca tcagaatcca ttgaaagcta atatggtaaa
     8641 aaaacctgaa gattatccgt ggagcagcta taatgattat atttccgcat atcaagggaa
     8701 tggcgtaaat attgatgctg atatgattat ggcatatttc aaagatcaga atgattttga
     8761 ggaattcaca aacacacaaa atgatgatat atttgtagat tttgaaccgg aagaaaaatt
     8821 tacagatgag gcactgggag atataattaa gaaaaaaata ggttctgttt ttttgtacgg
     8881 gaaatcaata ggagatcgaa atagactgat taaggatata tataatagta caggagcaag
     8941 catcaggcag ttagaacgcg tatttggagt aaataaaaac attatagcga aagctgtaaa
     9001 ataggacagg agaaacgtcc ccgtgtacca cccatgtacc aaaagagagg gttataatat
     9061 ggaatttata cctgcgaaaa caattatctc cggctatacg gaaaacaacc cgtggtttgg
     9121 taataattac agcatgaata tctacaaggg ctgctgccat ggctgcatat attgcgacag
     9181 ccgcagcgaa gatttgttaa attctattaa aaggcattct ccggtgctcg ttaagcatac
     9241 ggttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngattt gttaaattct
     9361 attaaaaggc attctccggt gctcgttaag catacggtta caactgctga tgattcgctt
     9421 tgcagtaaga ttgagcctaa tgctcccgcg gcttccagga ggtttgaggc tgtcagggat
     9481 ctttcgatga acggaatttt cacgggtata ctgcttatgc cggtacttcc gtttctggag
     9541 gataatgagg ataatataag caatattata gatctcgcat atgagaaggg ggcaaaattc
     9601 atatatcctg ccttcggagt taccctgcgg caaaatcaga gagaatggtt ctacagcaaa
     9661 attgatgaac attttccgtc catcaggcaa aaatatgtca aacaatacgg aaattcctat
     9721 aaatgcatat ctcccagagc aaaggagctg tggaaatttt tcagggcccg gtgtgacagg
     9781 cttggtattt tgtataaaat ggcggatata attaatgaat atcagcaaaa ctatttatat
     9841 gagcagattt caatgtttta gtccagggcg ggacaagaga aacgtccccg tgtctccccc
     9901 gtgtcttact attgacagtt gaattttgct atgatagtat aatgctgtta actttataac
     9961 tgaagactca tcaagagagg tggagggaag ggccctatga aacccggcaa ccggcagatg
    10021 tacggtgcca attcctgcag gattgtcatt atcaatcctg aaagatgagg gagtaaataa
    10081 taaatgtcat gctccttcgt cagaaggagt tttcttttta tacctattaa agaacgaatg
    10141 cttaaggagg tttattatgt taaaaaaatt gtttacatcc gaatctgtta ccgaagggca
    10201 tcctgataaa atatgcgatc agatatccga tgcagtgctg gatgcgatac ttgaacagga
    10261 ttccatggga agagtggcct gtgaggtatc ggtcacaaca ggccttgtgc tggtaatggg
    10321 ggagatatct accgaatgtt atgttgatat tcccaaaata gtcagaaaga ccattgagga
    10381 tataggctat acaagggcaa aatacggttt tgactgcgac acctgcgggg ttatcacctc
    10441 aatagacgag caatcctcag acattgctct tggcgttgac aaggctcttg aagcaaaaaa
    10501 gggagagatg actgagcagg atatagctgc taccggagcg ggagaccagg gcatgatgtt
    10561 cggttttgcc tgcgatgaaa ctcctgaact gatgccgatg cctatttccc ttgcacacaa
    10621 acttgctttg aggctttcac aggtcagaaa ggaaggcatc cttgactacc ttaggcctga
    10681 cggaaaaacc caggttactg tggaatacga tgacggaagg cctgtaaggg tagatacaat
    10741 agttgtttcc acacagcata gcccggaggt ggaccatgac actattgaga gagacattat
    10801 cgacaatgtt ataaaacccg ttatcccgga gggaatgtta gacaaggata caaagttcta
    10861 tataaatcct acgggaaggt ttgtaatagg agggcctcag ggggattccg gccttacagg
    10921 gagaaaaatc attgttgata cctatggagg atatgcaagg cacggcggag gagctttctc
    10981 gggaaaagac ccgaccaaag ttgacagatc tgcttcctat gccgcaaggt atgtggcaaa
    11041 gaatgtggtt gctgcaggcc tcgcatccaa atgtgaggta caggtggctt atgcaatagg
    11101 tatggcaaaa ccggtgtcgg taatggtgga aaccttcgga accggcaagc tgacggatga
    11161 tgagatagca aaaataataa tgaaggtatt tgatctcagg ccggcggcca taataaagga
    11221 tctggacctg agaaggccga tatacaggca gttggcagca tatgggcata tgggaagaac
    11281 agatatagat gtgccctggg agaagaccga taaagtcgaa gagctgaaaa aattagcagg
    11341 gatatgatac aatggatgaa gttcaaggaa ccatatgcga aatcatattc agaaatgaat
    11401 ataacggata tacagtgctg gatttggagt gcaataatga actgcatacg ctgataggat
    11461 atttttcctt cctgaatatt ggtgaaatca taaaggcata cggttcatgg gtgcagcatc
    11521 ccagttacgg cagtcagttc aaggtggaga cctacactac tgttactccg gcaacaatta
    11581 acggaataga aaagtatctg tcctccggca tgataccggg cataggcccc catacggcca
    11641 agaagctggt tgagaagttc ggccttgata ctcttgatat catgcagtac aatcccgacc
    11701 gcctgacgga ggtggagggg ataggagata aaaaggcggc gaagatatcc gaagcctttc
    11761 aggagcagaa agagctgaag gatatcatga tgttccttga acagtacggc atagggcccg
    11821 cttatgccgt aaggatatat aggacttatg gagcaaacac aataaaagaa ataaaggaaa
    11881 acccctacaa gcttgcggat gacatattcg gcataggctt caaaatggcg gacaggatag
    11941 caatatccat gggggtgtcc aagttctcgg aataccggac tgcatcaggt acaagatatg
    12001 tgctcaatca atatcatgga aacggacata cctttgtgcc ccaggaagag ctggtgagat
    12061 cgtcggcagc actgctggac gttgatgaag cccatataga agatacaata gtaaggcttt
    12121 ttatagataa caagctggtt gcggaaaaca tcggagacat ccggggagta tactccgttc
    12181 cttttttcag agctgaatca gggactgcaa gaaggcttat gcagctgttg tactacaaga
    12241 ttcctcctat ggatctggat atggagggcg aaatagcgga aatcgaaaaa gcggaaggaa
    12301 tagagctggc ggataaacaa aaaaccgccg tcaaggaggc tttaacccaa ggcatccttg
    12361 taataacagg gggcccgggc acaggcaaaa ccacaaccat aaagacaata ataagcattt
    12421 ttgagaagca tgggcttgag gttctgttgg ccgcacctac cggaagggca gcaaaaagga
    12481 tgcaggaggc aacgggcaga gaggcaaaga ccatccacag gctgctggaa ttcggatacg
    12541 gcgacagtga tgaggaaatg tttttccaga aaaatgaaga gtcgcccctg gagtgtgatg
    12601 taataataat tgatgaagta tcgatgattg acatactcct tgccaataac cttttaaagg
    12661 cgatagcgga aggcaccagg gtgatactgg tgggggatgt ggaccagctt ccctccgtgg
    12721 gccccggaaa tgtactgagg gacataatag acagcggagt gctgcctgtg gtaaggctgg
    12781 atgagatatt cagacaggct gaaagcagca tgattgttat caatgcccac aggataaacc
    12841 ggggggaacc tccggtatta agctcaaaaa acggtgactt ctttttaata aggcaggaga
    12901 gtcaggagga tattgttgca acaataattg aactctgcac cagcaggctt cccaaataca
    12961 caggcctgga tccctatgag ggaatacagg tgctgtcccc gatgaaaaaa ggcctgtgcg
    13021 gggtgctgaa tctgaatcat gtgctgcagg aggtgttaaa tccccggggc agcggcaagg
    13081 aggaaaagca gcacagagag gctgttttca gagttgggga caaagtgatg cagataaaga
    13141 acaattacag aatgaggtgg cggaatatca acaacttgga aacggaaggg gagggagtgt
    13201 tcaacggcga cctgggcact attgtgagca tagacaatga ggagttgtat atggaagtgg
    13261 tatttgacag ggaaagaaaa gtcaaatatg attttacaat gctggacgag ttggagcacg
    13321 catatgccct cacagtccat aaaagtcagg gctccgagtt cccggtgctt gttatgccct
    13381 taaccttcgg gcctcccatg cttatgacaa ggaatctcct ctatactgca ttgacaaggg
    13441 cgaaaagtat ggtggttctg gtggggaaag agcgattcct ttatcagatg ataagcaaca
    13501 atcatataat cacacgccat tcaggactga gaaagaggct aagtgctgca gagtaataaa
    13561 gaggtaacgg aatgattaag tatttgcttg acctgttgta tcccccaaaa ccaaaatgca
    13621 gtatatgcgg cagagccggg agattggctg tatgcaatgc atgcatggca tccctggatt
    13681 acctgcaggg aataacttgc cttcactgcg gcaaacagct gaatgaacag tatgatgatt
    13741 gcacctgccc ggactgtaag accggtatct tttattatga aagggcttat tcctgctttg
    13801 aatacggcgg tatgggtaaa aagcttatac ataagcttaa atacgaaggt atggttcagc
    13861 ttgcaaatgt aattgccggt ttgatggcgg aaaggcttga aaatgaaagg ttggtaatag
    13921 acgccatagt acctgtgcct atccataaaa aaaagcttga agcaagaggc ttcaatcagt
    13981 cgcaaataat tgcacgggaa ttgggggaaa ggctgcacaa gcctgttatg ggctgcctgg
    14041 aaaggatggt agagaccaaa gaacagtata atttagaccg taatcagagg tttttaaata
    14101 ttaggggtgc attttctgtc aaattatcgt ataatattgg taaatataag aatatcctgc
    14161 tggttgatga catatacact acgggaagca ctgtgaatga atgcagcaag ttattgaaag
    14221 atgccggtgc agccaaagtg tatgtgatta cggcagcgac cggcagcaat acgtaattga
    14281 gatgtgggac gtgagatgtg agaataagga ggtaatttta tggctgaaat aagaaactgc
    14341 cccaggtgcg ggaaaatatt cacttatata ggaaagccca tttgcggcag gtgtattgaa
    14401 tcggaggaaa atgagttcaa ggtagtaaag gaatacattt atgacaatcc cggagctact
    14461 atttcggaag tatcgcagga aacagaggtt tccgttgaga aaatcatgcg gttcctgagg
    14521 gaagaaaggc tggaaatcaa atctgagaac agcaatctca tgctggaatg cgagagatgc
    14581 ggaagagcaa taaaaacagg aaggttttgt gattactgca aggagcagat taacaaagac
    14641 ttcaagagag aattcggatt ggacaggaag gagcctgagc caagacctca aaccagggcg
    14701 gaaaaagaaa gaatgtatac cgccacaaga aggaagaacg attaaacaaa tatatgaata
    14761 ggatatgaag cagccgtgag gatataacat ggctgtttat ttttttaaaa aatgctaaac
    14821 taatataaaa gtaaatccga taaaatagtt gaaagatgaa atgcaaatgg ggtgttaatg
    14881 tgggatttaa tattaataaa atcgatgggg taagcggagt ctacaagaat cagaaggttg
    14941 cgtccaggaa ggatgttccg gaatcaaccc gagccagggg gaaaaaggac gaagtgcaga
    15001 tatccaaaga ggctatggat ttccagctgg ttatgaaagc agcaaaggca gcaagggaga
    15061 taccggacat aagagaagaa gttgttgccc ccattaaaga aaagcttgat aacggcactt
    15121 atgaagtgga cagcaaaggt atcgctgata agcttttagc ccgcaggtta aatttaaaaa
    15181 tatgaaatta accacagctt aaggccatta cctggctgtg gttatttcta tccagggggt
    15241 gtatacatga taaatgtcga caaagaagta aatgcagtta tagacatact ggctgaggaa
    15301 tatggctatt acagtgacct ggttgagctg tcaaagtcga agaaggacat cattgttgca
    15361 gggaaagttg cggaattgga caagatggtc aagttggagc aggacatgat ctttagtata
    15421 ggacaattgg agaggaaaag ggaggatgta atttccatgc tctgtaaggc gctggatatg
    15481 aacagtgagc gggtgaccgt acacgaactg gcaaaagtac tcaagcctga gctgaaaaac
    15541 aagctggagg atatccagag caagcttcag aaggctttat cagagctgaa ggctgcaaat
    15601 gacataaacg gacagcttat agagcagtcc ctggaatata tagattattc aataaacctc
    15661 attgcaggat ccggtatgga gacgggatcc ctgtacgagg atataggcaa gaataagagc
    15721 aaacaaggca aaaagaatat tattgatact aaggtataga ttaggattaa tggaaaatac
    15781 cggagcgcat acaatgagcc ttgcgcattt tgaggtgagt attaatgtcg tttagaggat
    15841 tgagcatcgg agtatccgca gtctttgcaa atcagagggc gctggatgtt accggtcata
    15901 atataagcaa tgtgaatact gccggatata ccaggcagat tatttctaac tcaaacgcct
    15961 tctatcagaa gctgggacat tcggggaacg gcaaggtgat gcaggttgga tacggcgttg
    16021 atgtacagga aataaggcag tatagagatg agtttttaga taacaagtac aagaaggaga
    16081 agaccggact gggttattgg gaagaaagat attcaagtgt aagagaactt gagactatat
    16141 tcagcgataa ctcggaggac ggtcttcaag aagtgatgaa caacttctgg aattcgtggg
    16201 agcagctttc caagcctaca ggtggtctga ctacaagggc aatggtaaag gaaaatgcta
    16261 ttgcctttgt tgaaactgta aaaaacatgg ataccatgct gcttaatttt aggaaaagca
    16321 aggataagga aatcattgac aatgcagcaa ggattaatga gatagcaaag gaatttgcga
    16381 ccctgaacct tgatataaaa aagatagagt ctcacggtgt tacagcaaat gacctcagag
    16441 accggaggaa tcttcttatt gatgagttga gcggattggc gaagatacaa attatagaag
    16501 gggattacat taatgtttct ttggaagggc gtttgttggt tgaaggcagc agatatgaac
    16561 agataaaaac agtacctgat acgggaaaca gcggatttgt aaggcttgta tgggagattg
    16621 acaatgggag tctatatatt tcaggaggaa gtattaattc aagctttgaa tcaagggatg
    16681 ttttggtagg gggcttccgg gagaggcttg accagtttgt aataggcatt gcggatgaga
    16741 ttaacgctgt acatattacc ggttacggta tatcggatac acttcaaaga cccttcttca
    16801 taaactatag agataatagt gctgtaggca taaatctctt aacaattgct tataatccgg
    16861 agcttgaaaa ttatgacagg attgctgctg ccgaagacgc atacagcaat gaggacaaca
    16921 ggatagcatt aaaaattgca gcccttaggt acactgattg ttttacggat gaaaagtata
    16981 caacaacagc cgcagacaaa aagtataatt atgatgaatt ctataggaat atcatatcag
    17041 atttagggaa tatcggtcag gaggcgtcta ccgcagctga tgcacagaag cttttggtag
    17101 atcagataga ctatagcaga caagcattaa gtgctgtatc tttagatgag gaaatgacca
    17161 accttataaa atatgagcat tcctacaatg ctgcaacaag aatagtgaat gtaattgacg
    17221 aaatgattga aatgattgtt aacagaaccg gtttggtagg aagatagcgg ggtgattata
    17281 tgagaacaac attcggatcc tttaatattg caacttcagg actctttgca agtcaaaggt
    17341 ctcttgatac tacaagtcac aatatatcca atgccaatac agatgggtac tcaagacaga
    17401 taagcaaaca aagggctacg atgccgactt acggggaccc tacaggggta gtgggtacag
    17461 gagtggagac ttacgacatt atcagaatga ggagcagtta tcttgatacc aagtactggg
    17521 gacagaataa aacttatcag gaatggaatg tcaagcagac ccagcttgag aatcttgagg
    17581 ggctgtttaa tgagccctcc gacacaggta taagaatcgt tatggacgaa tttttcacag
    17641 ctcttgaaga gctttcaaaa cagccgggag acagtacctg tagggtgaat gttgtagaga
    17701 aggcgaatat gcttaccact actataaata gaaacggaca tgagcttgtt aactcaataa
    17761 gggatgtgaa cttttcaata aaaaataagg tcagccaagt aaattcattg acagagcaga
    17821 tagctaatct gaataagcat atattcagct ttgagttggg agggaataag gctaatgacc
    17881 tcagggatca aagaactttg ctcttggatg aactttcatc tataataaat attacagtta
    17941 tagaaatgcc tggtcccaat aacaataatt atatggatgt aaaagttggg ggtataaccc
    18001 ttatagacca tatacatttc aacaaattag ctactgagga tgaaacgaaa gaagtttcag
    18061 atttaggagg cggaaaaatc agcaaggtgg tttgggaagg tttggacaat caggaggtaa
    18121 atataagcag cggagaactg agaggcttgt tggatgtacg tgacggcaac ggagaagatt
    18181 acagctacag aggaatacca tactacctta ataaactgaa tgagtatgta aaaggattta
    18241 caacagcgtt taatacacag cacaaaatgg gagttgatct ccaaggagat ccggggcaga
    18301 acttttttta cgaacccggt attcctgaag taaattgcat aagttttaag gtgaattctg
    18361 atattatagc agatccaact accatagctg cttcatccca gggaaacgga gagagtaata
    18421 atgataacat taagcttctt atagcactta gagaaaataa aggtatgttt tcatcactga
    18481 taggtactcc ggatgacttt gtaaagtcga tactttccgc tcttgctgta gacagcaacc
    18541 aagcaaagag gatgactacg aatgcacaag cattgacaga tcatatatat aacagccgtt
    18601 tgtcggaatc gggggtatcc ttggatgaag aaatgacaaa catggtaaag ttccagcagg
    18661 cttacaatgc ttccgccaga atggttacaa ctttggatgc catactggat acgaccgtga
    18721 acagattagg attagtagga agataatgag gtgatggtat gagagttaca aattctatga
    18781 taattaataa tctgatgagg aatctgaaca aaaacctcaa cagaatggaa aagacacaga
    18841 gaagcctatc atcaggcaag aagtttgtta atccttccga tgaccctatt ggtgtatcga
    18901 gaagcttgag gctcaatacc gaagtggcga caatgaatca gtacaaaagg aatgcagatg
    18961 atgtgctgtc atggctgagc actaccgaga tggctgcaag caatatcact gaagttctga
    19021 aaagggctaa ggagttgaca gttcaggctg ccagtgagac aaactctata gatgagagag
    19081 tatcaatatc taaagaaatt gaagagctta ggaaccaact tatacaaatc gggaatacaa
    19141 cctatgcagg aagttatctg ttttcaggct ttaagacgga taaaccccta ttggctaaag
    19201 atggaaccta tgatttagga gacagtacaa atatattatc ttccgaagag attattgaag
    19261 taaacatcgg cgtaggagac agaatcggtg agaattttgt aggtcagagg ctttttgggc
    19321 tgtactcagt tgctgccgat ttggatttga aaaacgccga aagcattaaa cagcagcaaa
    19381 taattacggg aagctttctg gatcttgcaa cgaatccgat tgatacagca accagctttg
    19441 atattactta tcaaggagca actactacaa taaatctgcc caatgttcct tataacgata
    19501 ttgctgatgt ggaaggtgac atcagcgctg caataatggc ttcagcagat ctgaacggac
    19561 atgttaccgt ttcggttgca aataacaaga tagtttttga agctgacgga gagttcagcg
    19621 ttcaaacggg tacggctatt ggaattagtg ataataagaa atctgtatcc aaggtaagct
    19681 caggggataa atctcagatg atagctatct ttgatcaatt gttagcagat cttattgctg
    19741 atgatactgc aggcataggc aacgcattga acaggataga aaaccagttt gaaaatgtga
    19801 atgcaataac agccgagatc ggtgttagga tgaacagggt ggagttgaca agcaatagga
    19861 tactggatga cacactgaat ctgaaggatc tgctttcaaa gaatgaagat gcggatattg
    19921 cggaggttgt tatgaacctt aaaatggagg aatatgtata tcaggcttct ctatctggag
    19981 gagcaaagat aattcagccc tcacttgttg atttccttag gtaaagcgaa tgattaagaa
    20041 tgaggtgaaa atatgataat aaaaacaaag tttttcgatg agattgagat tagcgacaag
    20101 gacatagtgg attttgatga agggatacca ggctttccgg ggacgcatcg atatgctata
    20161 ataaatgatg ataatacatt tatcagctac cttcaatctt tagattcaga ggatgtatgc
    20221 ttcattatga tacctcctgt tttcgctctc aaggattatg atattgaaat cagcgaaagt
    20281 actgtaaaga agctgagtat tgaaaggcct gaagatgtca agctgtatgc gatacttacc
    20341 atactcggca atttcaagga tgctactgcc aatctcaagg ctccgatagt agtgaatgtg
    20401 aataataaca aggctgttca ggagatactt gatgatgaga ggtacggcat acgtcataga
    20461 atagtaaagg agtctgatgt ctgatgctgg ttctgacaag gaaaaaggat gaagctgttg
    20521 ttatcggaga tgatattgaa ataataatat ccgaaatatc cgaggataaa gtaaaaattg
    20581 caataaaagc tccgaaaaat ataaaaattt tcaggaaaga actgataaag gctgttgagg
    20641 atgaaaaccg gaagtctgtt gatatgtcgg atcttgacat tgacggactg cgggaagcta
    20701 tcaaaaagta acaaaaataa ttatttaaat gctataaagt tacaaaacat tccaaccgat
    20761 atataaatta gaacataaga ttaattaaca gcatagggcc catggtgtgg attgatctta
    20821 tgaatatatt gccggaaagg aatccggcta aaaaaatcaa ggaggaagaa caaatgagaa
    20881 taaataataa cattatggca atgaatgcac agaggcagtt gggcgtaaac tcaacagggc
    20941 agagcaaggc attggagaaa ctcagctccg gtcttaggat caacagagcg gctgatgatg
    21001 cggcaggtct gtcaatatca gagaaaatga gaggccagat aaggggctta gcacaggcac
    21061 agagaaatgc tgaagatggt atatcattga tacagacagc agaaggtgca ctgaatgaag
    21121 taagtgatat gctcgtcaga atgaaggaac tgggtgttca gaaattaaac ggaacgtact
    21181 ctgccggtga tgttgctaac atgacagcag aactgacaag acttaattct gcaataacca
    21241 gcattgtagc ggatacaaaa ttcaatggaa cacagcttct taagggtgga actgttaatt
    21301 tgatgctcaa ggatactgga actgcaacag catataatct tgcaaatata ggaacagttc
    21361 ccgcagcaga tgcagatttg gcaaccattg aaacagcaat taataccgta agtacacaaa
    21421 gagctaatct tggtgctgtc cagaacgagt tggagcatgt ggtaaagaat cttggtacaa
    21481 caactgaaaa ccttcaggca tcagaatccc gtataagaga tacagatatg gccaaagaaa
    21541 tgatggcttt cacaaagctc aatattctta accaggcagc aatatcaatg cttgctcagg
    21601 ctaaccaggc acctcagggt gtattgcagt tactgcagta atacaaaagc cagaaactcc
    21661 ggatttttcc ggagtttttt tttacacaaa agggcatcag gtctattatt aaggtatgtg
    21721 gaatatttac cgataacaat aatagagtat tttattactt ggaggttata atatgcaggt
    21781 atggtttttg gataggacat ataactatag caacgaatct gcttcggtaa aggaattatt
    21841 tgataatatt gacagattgc tcgctgatcc tcaatatatc cttgcaggta tgataattga
    21901 tgatatggag atctatgaaa actacgttga gtacattacc aagaggatcg atagaataaa
    21961 aacaatcaag gttgtaattg atacagtaga aaataccata gacagtattc ttgaatcaat
    22021 gagcaaatat atggataggg ctgttccgga gcttgaactg cttgccggta gtttttattc
    22081 cggtggggat gaagaggtca gcatagaaaa gctggggcag cttgccgagg ggatacagtg
    22141 gatccttaat atgatatact atatagataa taataaagcc ttgcacagtg ttttaaaaag
    22201 caatagtatc tggaacgaat gttcttcata cggaaacaga ttaggcgaag agtttaataa
    22261 tctgaaagag gccatgggca atatagacat gatacttatt gccgatattc tgaggtacga
    22321 aattactgag gtttttacgg gtattaacca acgtatcaaa caaatatacg gtcatggaga
    22381 cgagggaaat gggaaccgat aattctgcca tactcagaac aagatttgaa agcctatggg
    22441 aaagcttgaa caaaacaaca gaaaacgttg gtccaaatgt agtaattgaa aatgcaagaa
    22501 acagtatgta tacactatat gtggaaaata gcggtaacaa gctttatata catagcaggt
    22561 ataatcctgt caatgaggct gaaatgtttg ccgggcaatt caaagatgtg gataaatatg
    22621 accatgtgat attttatggt atcggaatgg cataccatat tgaagccttt ttcagcaaat
    22681 ttggcaataa gcatttttca atatatgagc ccttccttaa tatttttaag agctttatat
    22741 catacagatc catggatagt attcccttgg agtatctgga caatattttt ataggagaca
    22801 gcctggagga tccttatgaa tgcttggagg cactcctgaa agaaatagaa ggggaagttc
    22861 ttcttgtaat tctgccaagc tacgaaagaa ttttcagcca ggagtacaaa agcttctatc
    22921 aaaggttcgg aagcattctg gacgatacaa aaacactgaa taggaccaac cggttgtttg
    22981 aaaagctcta tgtaacaaat ataatgaaaa acttcatgga gatcatagac agccctaaca
    23041 tacttcattt aaagaataat aaatttgcag gtaatccggc aatattggtt gcggcaggcc
    23101 cttcactgga atatgagctt aaaaacctaa gatacataaa gaaaaaggga ctggcctatg
    23161 ttttatctgt gggctcagca ataaatacac ttttggaata tgacatatat ccggatgcag
    23221 ccttgaccta tgatccacag aatcacaacc acatagtatt caaaaagctg aaggataagg
    23281 gcataacaag catacctgta gtgtttggca ccattgcagg ttacggtact gtacaagcct
    23341 atgaaggacc aaagctgcat tttatcagca gcaatgatca tgtagcacca tactttttta
    23401 aaccggaaag tgctaaagaa tttgatactg ttcatgatgc tggatcaatt gctgtagcgg
    23461 ctatgcaaat gctttacaag cttggctgcg acccgattat tctggtaggt cagaatcttg
    23521 catataaaga caggaaactt tatgcaaagg gtatagatta tggcagggat ttatcggaaa
    23581 cagacaacaa gtatctggac agcagcttca tggtagatga tgtagaggga aataagattc
    23641 aaacaaataa agtattaaac cattacaagg atgaatttga aagatgcatc aagcagtatg
    23701 gcattgaaaa tataataaat accacaagag gtggtgcagc tattggcggt acggtatata
    23761 agccgttgaa ggatgtaatg gaaaatatgc ttgtaagcag tatagttgat gctgaatggt
    23821 ataaggacga gggcgtatct tatgatattg actatatgat aagcaggtgc aaatcaattg
    23881 aagctgagca aagacatctg cttgacatat taaagagtat aaaaaaaatc gctgcacaaa
    23941 taaatgggaa tacaaactca gtagaggctc cggaatcatt gttttcattt ttttatgcag
    24001 aatttaacaa aatgttacaa aacacttatt ttagtacatt catatatccc atgaattatt
    24061 atcaatgttc attgttgaat agaaaaatca acagtataaa cactgaaaag aatattgtac
    24121 tcaagataca aaagctgtgt agagagatgg agccgtttat tgatggctgc atcaatgata
    24181 tgcacctgat ttcaccacta tttggagata tgtacgaagc gattatggat aacgatataa
    24241 aaataatgac ttttactatt aaattatgct cttaaaacct caaaatgttt tggggttttt
    24301 tcaattgtgt tacagtacgc gatatgacga atgcttatat aatataaaga aacagttgga
    24361 ttttgtcgat atttataaat atggagtatt taatgcaaag cgggtggaac aatgtttgag
    24421 aacaagaaaa tacttgttat cggcgggaca ggtacgatag gacaggcttt ggtgaacaag
    24481 cttatatcct ataacccaga agtaataaga attctcagcc gggatgaatt caagcaatat
    24541 cagatgcagc aacagctgga gggctataag aatatacgtt ttttgcttgg cgatgtaagg
    24601 gataaagaac gtatctacaa agcgatgcac ggaattgata ttgtttttaa tttggctgca
    24661 ttgaagcatg tgccctcatg tgaatacaat ccatttgaag cggtaaagac aaatgtattg
    24721 gggactcaga atgtgattga atgtgcaata gaaaacaatg ttaagaatgt aatatatgca
    24781 agcagtgaca aggcagtatc ccctacaaat acaatgggcg ccaccaaact attggctgag
    24841 aggcttattg catctgcaga ctattggaag ggcggaaaga taccggtatt tgccgcggta
    24901 aggtttggaa atgtaatcgg ctccagaggc tctgtagtac ctctctggga ggagcagatt
    24961 gtcggcagga aagagatgac cgtgacagat cccaatatga caagatttat gatatctata
    25021 ggttctgctg taaatcttac cttaagggca tgtgagattg ccaagggtgg agaaatattc
    25081 gtattaaaga tgcctgtcat acgtctggga gacttggccg aagtggtgtt ggataaggtg
    25141 tgcagcgaga agggaatagc aaaagatgaa gttaacacaa gggttgtcgg actccgtccg
    25201 ggagagaaga tgtatgagga gctgatgact gtagatgaag cacagtatgc ggtggaattt
    25261 gatgatatgt ttgctattca gcatttatca aatattacgc tgcccagtaa caagcctaag
    25321 gaattcaggt cagatatgtg ctcaatagta gaaaagtcaa gtatatggag tatgttaata
    25381 gattgaatta agggtgccga gggaataata tgaaaaactt ttttataaag cataaggata
    25441 agatattgtc aggacaaaaa gtactaaagg aatatgatat ttttgaggaa atagacacga
    25501 ggctttctga ctcatatctt ttcgacagag agaacaattg tgtaaaatta ggttatagta
    25561 ggtgtaaaaa aattggtttt gataatctct tactctatag atttgggaca gtatcaagag
    25621 atgatgaaac tttaattttt gtaccgcaag ttgatgaaca aattggagac tttataaaaa
    25681 ggtcgcaggt tttcttgaat aatccagtat tgccggatga aacaccagtg atacatctta
    25741 attttgatat acatgagaaa atagaggcgt gtatttggct tggtaataaa tatgcttatg
    25801 aaacatatga tatgtacttg aaagggatta ttccatggga taatgagaga aagagaagta
    25861 ctgaattcaa gaggaccggt atctttatag gtacctcaag tattcatatt atagagtaca
    25921 gcggaacgga agcagaacat aaacatatgg tatttagcac ccctaattat ggtaacgagg
    25981 ttgactgcta tattgacttg aaaaaaggtt attttaggtt agggcagaat ggcagcaaga
    26041 tctatcatag tggactaaag caggatgaga gctgtagtat taatgtttcc tgtaattcaa
    26101 gagataatca ggtcttttcg tgtactaatc cgggattgat tttaagaagg tttgagttat
    26161 gcagaaatca aggagtgatc tattttaaat ccattgatgc ttctttagcc aaggagtctc
    26221 gtgtaatgat gttatataca ggggatattg cagttcaata ttttaatgaa aaaagcaatc
    26281 aatggcattt tgtgaaggat agcatcctat ttaaagatct tcataatgta agcctaagag
    26341 ctattatgga taccgatgac agaatatatg cgttgatatt gctgaaggtg atatagaaag
    26401 caaagagtaa ttattgatta gaggtgacag aatgcagcca agagtagtta atgtatgtga
    26461 tgttaaaatt ggaacgaata atccacttgt cctgattgcc ggaccatgtg taattgaatc
    26521 tgaagaaata gttatgcgta ctgctgagta tataagcagg ttagctgaaa aattaagagt
    26581 atcattcatt ttcaaatctt cttttgataa ggcaaacagg tcttcgataa cgtcttacag
    26641 gggacccgga attgataatg gacttaaatt actggataaa gtaaaaagta gctttagagt
    26701 ccctgtgata acggatattc actcggctga acaagcaatg cctgtctctg atgtggcaga
    26761 tattatacaa ataccggctt ttttatgcag gcagacagat ttgctgactg cagcagccag
    26821 gacaaaaaag cccgtgaata tcaaaaaagg acaatttatg gcaccgtggg atatgaaaaa
    26881 tgtgatagat aaaatactca catatgataa tactaatatt ctcctgacag agagaggcac
    26941 aagctttgga tataataatc tggttgttga tatgacatct atagttgaaa tgaggaaatt
    27001 tggttttcct gtgatatttg atgcgacaca cagtgttcag aagccggggg caatggggac
    27061 aacaagcgga gggaattgtg aatatgtaaa atatctggct caggcagcaa ccgcaatagg
    27121 aattgacgga ttatttcttg aagtacatcc tgatccaaat acagctttat ctgacggtcc
    27181 gaatatgata gcactaaaca aactggagga gctgctggtt acaattatgg aattggatag
    27241 gacaattaag ggtggaattt aatgagtaga gtaaaaatgt tgattatgga cgttgatggt
    27301 actttaacag atgggaagtt atatatatcc ggttctggtg aagagatgaa agcatttaat
    27361 gttaaggatg gatatgcaat aaaaaatata cttccaagta tgggaataat ccctgttatt
    27421 attacaggca gaaaatcgaa aattgttgaa attagggcta aagaactggg cattttggaa
    27481 gtgcatcagg gtatagaaaa caaagaagaa acctacatca aattgcttga tagttacagt
    27541 tgtcaagatg aggatatagc ttacatgggt gatgatatta atgacatacc aattttaaaa
    27601 cgcgcaggta tcagcgcttg cccatgggat gcttcagcag ctgtaaaaag tgtttgcagt
    27661 tatgtgatgt caagcaaggg tggggaaggc gctgtaagcg agtttatatc acatttgcat
    27721 gaagaaataa ccgggtctaa actcggtgtg ctgtaggttg ctttagtgat tatgaattga
    27781 ggtatttatt ttgaaaaagt gcgatttttg cggtagcatt atgaatttca tatataatat
    27841 gaacaattat gaaatagtac agtgtgacaa atgcctgact tctactgttc ttagtatgcc
    27901 gcaggatgat gaaatagaag atttctattc gggatttatg ttttgtgcaa atgcatccaa
    27961 taaaagtata ttcgaagatg agacttttaa taagtggttt gattcattta atctaggttg
    28021 taatgcgaaa atgctggatg taggaggcgg gggaggattt ttctcttatg catttgaata
    28081 ctttgataaa ggagacagtt attatataga tattgacaat gaggcatgcg aatttgcagc
    28141 gcaggaactg ggcttaaaaa atgtgataaa tgataatgtt aagaatctaa aggacagaac
    28201 gaatttaaag tttgatttta tatatgcaag acatctgatt gaacacctta aggacccttt
    28261 agagttcata gactcttgta ttgagctgtt gactgcatcc ggggttctga ttattcagtt
    28321 tcctaatggt gtgagttttg aatatctggg ataccctgaa ttactaaaaa gccgggtaaa
    28381 aaccatacaa agctccaatc gtaatttctc aagaatagag acattactta ctatttgcag
    28441 taataaaata gctcatggta tagatcctat aaggcatttg tgggccatta caccaaaagg
    28501 catttcaagc tatctctcag gaaaagatgt tgattttgaa attaaaaatg ctccattaac
    28561 ggatcctgtg tattctccat actacaaggc aaaaggtgta tataataagg ttagatcaag
    28621 catcattaat aatagcttag tcaagataaa tggaggtact catcttatcg ttataattaa
    28681 aagaaaacaa tagatgatta ttgatatata aaaaggatgg tactaatgga caacaatgtt
    28741 ttattatcta cattgaaata taattattgt atcggttgtg gaatttgtag gatgtcgtgc
    28801 ccatatgaag ctataaaaat ggttttgaca aagaattatg atataaatcc caagattgat
    28861 aaatccaagt gtattaagtg cggaatttgc acagaatatt gtcctcatac aaaagaaaag
    28921 cttttatctg aaatacaaaa agtatgtgaa acatcttcgc caaatagctt tggtcttgaa
    28981 aaagccactt atttattggc gcatgacaaa gatgcagaca agagaaaaaa aagtgcttca
    29041 gggggagtgg ctacagcttt agtagagcat ttgtttaaga agaaaattat tgatggggta
    29101 atccatgcaa caatgagact tggttcaatc ggagaattac attatgaagc atcaataagc
    29161 aattcctttg tagaggctga taggaaaaga agctcatatt atgggcagat agattattca
    29221 catatagttg atgaaataaa aggcaaggaa aagacatact tagttatcgg cgttccatgt
    29281 gcaatcagag cgctgacaaa gctatttaaa gaacacagct catacaataa gaataaaata
    29341 tatacgcttg cattggcttg cagccataat gtcaatggac aatttgttga ttttctggct
    29401 gaatcgctcg gcataccaaa ggatatacaa tttaaagcaa atttgaggaa taaagataat
    29461 atcaaagatg caaacaattt caacaatcat ttcttcaatg gacaacaaga tatagtaaaa
    29521 attaacagat tcaaatctct atttactgat atatggagga attacttttt ttccatgaaa
    29581 gtatgcggtt actgctcgga tttttgggga tacactgcag acataagcct taaggatgca
    29641 tggggcaagt gggcagataa tccactgggc gacagtatag cagtgattag aaataagaaa
    29701 ttattggatg aacttttgga ttctgataat atcgaataca aatatttgga ttttgaaaca
    29761 gtagccggca gccaattgag cacaacacta tacaagcaag ctaaagcggc ggacaggctt
    29821 aataaaccca tatggcatat agacaatatt aggagtggat accttaagca cactgtttta
    29881 tcggaacttt caaagttctg ttacaggcat tttggctatg aattgtcgaa gaggattcta
    29941 tttattgtgc ttgctgtact ggtcaagctc gagaacaatg cccgaaatat agagaacatt
    30001 ggatttctta agagaaaagc acttattaag gatattacaa aaaaagctga taataaaaga
    30061 gtagtttttt ttggaaccgg taagatggct gaaacactga aaaaagctat tccggtgcac
    30121 gttgaatact atgttgataa tgatgaaaaa aagcataata ccatgattga taataccata
    30181 ataaaagatc caaatgtaat actttatgag aatagggatg atgttctaat tattgttgcc
    30241 agtatgtata ttgaccaaat atccatgcag cttgagaaaa tgggccttat cccgggagta
    30301 catttcattg acgggaggat attatttgaa aatatagccg gaaaaccgaa acattttaag
    30361 gaaagcaaat ttgcaatggg tattgaggta attctaaaac cttttatagc tgtgtttatt
    30421 aaagctaaaa gcatatttgt gtatgagaaa acaaagaagc ataagaaaat ccttgtggta
    30481 ggcggatacg gttacaaaaa tacaggtgat gaggctcagt taagcgctac aataaggtca
    30541 ttgaaagagg agtttgaggg gtacattata aaggtgctaa ccccaaatca gcattatact
    30601 tattttacac atggcgaatg caatgtaggt gatgcgccga gggtggcgtt ttacgatgca
    30661 ggggagactg ccttgtatta tttggacagt acttacaaga agctaaagtt tatttttgtc
    30721 tctttattgg tttattggaa tgcctttttt gttagggcgg gactgccttt attcttaatt
    30781 aatgcaaaaa aggcagcatt gctatatgag atttctactt cagacatgat atatttcagc
    30841 ggtggaggct atttaacagg aaaaactgta tctcggttat gggatagtat gtttcttatt
    30901 agaatagctg atatatttag ggtaccggta tttctgtcag ggcaaaccat aggggtatgg
    30961 aatggcagct ttaataagcg gctgtcaaaa tggggactgt ctaaagcaaa gctcataacc
    31021 ctccgagatt cttatgagtc tgtaagggct ttggaagaaa taggaataag tggggagcat
    31081 gtgttttcaa cctttgatga cgctttattc tgcgagaagc tggaagatga gagtaaaatt
    31141 aataatttac ttatgaattc agggataagg gatgaacagg ctaataaagg ctatgtagcg
    31201 attaacatac attattgggg cttggacacc agcgaagaaa agagacaatt actggagaaa
    31261 atagacaaag ttgtcggcat cgttttgaga aaaactgatt gtaatatcct ttttgtaccc
    31321 atggtgccaa gtgatgagga agcaataaat gattttatga aagcttatag gaaggataga
    31381 ttatacaatt tgtcatatga ctatgatttc cgaacagtaa gggccttgat cggcgggtca
    31441 gatatatgtg tcacgatgaa gcaccatccc attatatttg cgttaggaga aaaagttcct
    31501 gttataagct tggcattagg tgattactat gagcataaaa atatcggagc tttggggatt
    31561 ttcggattgg aaaaatgcaa tataatattg tcagatgcgt tgtattatag agactttgat
    31621 ctaattttgg atgaaattta ctcggctaaa gaaaacatta tacggaaaat agaggaaagc
    31681 tataaagaag taagacagaa gaaggcatac tttatatcca ggattaaaga aatcattagt
    31741 aataagttat gatatcataa aaatatcacc atcggaggat ataatggaat ttgattattt
    31801 taatttattt aataaagtaa aaaatattat atatgaggca caagctaaaa agcttgtaat
    31861 atttggcacc ggaagctttg cgaagctttt gtctgaatat atgccgaaga agccgtcata
    31921 ttatgttgat aataatatca ataaactaaa tcgtttattt atggactccg atatacatag
    31981 cccggatttg ctaaaaaccg aggacagtaa taatttatta atccttgttg caagtatgta
    32041 ttacgaagaa atatcacagc agctgaagag catgggatta aatgagggag agcactttga
    32101 aaacgggagg ccgctatatg atttgattaa tgtatataaa agctttggtt tggaagaaga
    32161 tcttaaagag tatattcgca aagaacaatt gattacttct tcttgcacag atcctcacaa
    32221 agttgtgctt gcaaataagg aatgtgagtg gctgtgtgct gataatgatc gattatattt
    32281 aaaagggctg ctcgagggat acatgctagt atttgttgag gataaccgta atacacacaa
    32341 tatatatttt gagaaaatgg ataaaagctt cagacagatt gagtacaagg gaattaatct
    32401 gtatgaggta tctatttata atatttgtgt tgatttgcag ttggttataa ctgatattga
    32461 catatcctta aatgagcata aggatgcgat tagaaagtgg tataacatta gtgccaatta
    32521 tataaataca atagaaaacc ttactgaatc tcttaatgtc acaagcgtaa tgattttgca
    32581 aggatatctg tacacttcag ctattttgcg aaggtttgca ttgagtaatg agatgcctat
    32641 tctggcattg gagaatacct ttaacaagac taaagcaatc tgggataaca tatcaggtat
    32701 aactgtaaat aagaatttag ccagaagcta ttattggagg tattatgatt cggtagattt
    32761 ttcaacagca tttgaatatt gcatgaagta tatcgacggt atcaaaaact taaagatgga
    32821 ggagcatata acaccttcaa ataagttcct ccaaaagagc ggcagaaaaa ctattcttta
    32881 tataggtcaa gtatattcgg attcatcggt attatttggc atatggaact ttaagaatcc
    32941 tgtagagata attaaagtat tagtagatta ttgtatcaat aatgacttcc aattgatagt
    33001 gaagccgcat ccaaaagaaa aggacctggt tgatgtatta ggtaataagt atgacaagct
    33061 cacttttaga atgattaata aagataaaga attggcagag aaaattacta aatatggtgt
    33121 tgttgtggac agtgaaaacg aatatgatac atattcactt atagattact cggatttatg
    33181 catcacaata aattcacagg ctgggttgga agcattgata aaaaataaag acgtgatact
    33241 ttgcgggaat tgcagctatg gaggattagg attctcttac gaagctcaca acaaggatat
    33301 gttgttgtgc tttatggata tggtattgaa gaaaggcatt tcccttataa ataaagaaga
    33361 tatttataag tttttttaca tatttaatga gaaatactgc atttcgaaag atgctgaagc
    33421 tatcatagat aaactaaaaa ctatcgagtt tggtctgaat aagaaaaatg cagggagaaa
    33481 ttgatatgaa taatgggctt tatgaaggaa atataagaag agtaattcag caagagggag
    33541 aaaaactgct gcaaattgcg gataaccttg actatgaaaa ggtgaatatt gcggtagagc
    33601 tgctattaaa ttgtaaaggt aagatattgc tgatgggggt tgggaaatca ggctttgttg
    33661 cccgcaagat agcagcaacc tttactagca tcggtactcc tgcgatatac ttgaatgcaa
    33721 cggatgctat gcatggagat attggcattg tttcatcctc agatattgcc atagtaatca
    33781 gcaacagcgg tgaatcccat gaaataattg aggtacttcc ctttatcaaa aatagaaatg
    33841 tttcaataat agcgttagtc agcaatgcga agtcaacttt agccgagaat gcagatatag
    33901 taattgacct tatggttaag caggaagcat gccaatataa tttggtgcct acgaccagtg
    33961 ctgctgctgt aacggctttt ggagatgctt tagcattaac cttgatggag gcgaagaata
    34021 ttgggcctga gaattacgca ttgaatcacc ctgcgggcag gctgggaaaa aggctgacat
    34081 taagagtaaa ggatttgatg catgttaagg aaagtaatcc tacgatatat ggggattcca
    34141 atttgtatga aattataaag gcaataagtg atggcgggct tggggctgtg agtgttatag
    34201 ctgaagacgg gcatcttatt ggaatagtaa ccgatgggga catcagaaga gctgttcaga
    34261 ataacttacc aaataatatt tacgatttat atgccgaaca ggtaatgaca aaggatccaa
    34321 ttaaggtgac acaagacata ttggcatttg aggcaatgaa cattatggaa aaccgtatat
    34381 ctcagatatc tgttcttccg gtagtggata ataataatgt ttctataggt atgataaggc
    34441 tacatgatat tgttaggagc ggattgtaat gaaaatactg gcagttattc ctgcaaggta
    34501 taactcacaa agactccctg ggaagccctt ggcgcttata ggaagcaaac ctatgataca
    34561 gcgtgtatat gaaggaacta agcaatgtga gttttttaat aaagttattg tcgctacgga
    34621 tgatgacagg attaaggagt gcgttgagag cttcgaagga gttgctatgc ttacagatac
    34681 caaccactca aatggcactg agagagttgc cgaagttgca caaaggtttc ctgaagcgga
    34741 tataatcgtg aatattcaag gagatcagcc ttttattacc tctgatatac taacggaact
    34801 aatccaccct tacttaaata atcaaatgcc tgatatggta actttagcct gccctcttaa
    34861 cgaggagctt gattacacca atcccaatgt agttaaggta atatgcagta caagcaataa
    34921 agcactgtat ttttccagag cagctatccc atactataga accaagctgg aagcccctgt
    34981 atatcagcat ctggggcttt atgcctttac tagagagttt ctgttgaaat atgtaaggct
    35041 tgaacccact cccttggagc aatgcgaggg cttggaacag ctgcgagcat tggagcatgg
    35101 ttataatatt gttgtcagga ttattaaaga gtcaattatg gaaataaaca caccagagga
    35161 tttagtaagg actgagttaa agaaaaaatt tatataaatt catactggag gaacctaaga
    35221 tgattttttg tacatcagta tgcgccaatt atttgccaaa agccatggtt ttagcagaat
    35281 cactgaagaa gcataatcct aattctgaat ttgtagtatg tctggtagaa aaaacaatag
    35341 aggaggctgc tgaaaagttt gagtattttg actatgttgt attatcaaaa gatattggaa
    35401 taagagactt cgataatttc atattcaggc atacaattgt agaagcttca acagcggtga
    35461 agggtgattt atttgagtat cttctgaata attttaagga tgaagataag tttgtatatt
    35521 tggatcctga tattatggta tacagcagtt tttcagaatt ggatagcata ctggagaaaa
    35581 atgaaattgt attgacaccg cacacatatt ttcctgaggc aggagaagaa ttcaataatg
    35641 gaatttttct tgaatgcaac catctgaaac gcggggtata taatctgggt tttttgggaa
    35701 tcagaagggg agaagaatct tctaagttta taacctggtg gaaaaaccgc ttaaatttgt
    35761 tgtgctatga tgaggttgat aagggcttgt ttgtcgatca aaagtggata gacttagccc
    35821 ctgctttatt taatgtatat atactaaagg aacctggtta caatttggct tattggaacc
    35881 ttagcaagag aaatatttcc atacaagagg gtaaagatta ttacgcgaac gacaaaccat
    35941 tggtgttcgt tcatttttcc ggttgggatt ccggatttaa taatactgcc attgcaacct
    36001 atgctcccaa tgtaaatgag ttaatacatg aaataaataa acaatataat gcacaactat
    36061 cagagatgga caagtataac ctgagcaaag caaaatggag ctacaattac tttgataatg
    36121 gtgacgaaat tcccaggggc attagaatta tatataggaa tagtggagtt ctccagcagt
    36181 tgtatactca tcctttcggg cagttgaatg gagaattggg ggaatacctg gaaggattat
    36241 ataatatcaa ggacaacaat cccgaagcag acaaatggtc aattattgac aactatataa
    36301 atgaggtttg cctattagac aaagagtgga aagcaaaaaa gagaattgag ttaggcatct
    36361 taaagctaat tcttggtggt gtagaaaaga aatgcataat atggggtgca agcaacggtg
    36421 gtaagataat gaaaaaagtt ctggatgttt tttatgaaga tgttaagatt gccggattta
    36481 ttgataaata caagaccggg gagtttgaag gatacaatat ctataaacct gggcagctaa
    36541 aagaattgag ctttgattat gctttcatag caacttctcc cgggaaagca gatgcggaaa
    36601 aatatttgaa atcaaacgga tgtttacctt ataaggatta ttattcatta ttttagtgaa
    36661 tatgtttatt agcgcactta atggagggta taaatggtaa gaagtgtttt gataattact
    36721 aaatgcattc gaatggatgc ggaaggcgcc aaactaataa aaagaattaa agggctgata
    36781 gaaaataaca tttcggtaat tgtggcttct catggaggaa atggtgctgc agagattgct
    36841 gaagcaggag ctgagtatta taggttcaga tggttgaata gtgaacctga tgaattagac
    36901 cacaaaacct tcgttgaaaa cctgagtatt ataaaaggta taattgataa aaagcaaata
    36961 gacataattg aagtaaccga tttatatccg gccctttttg tgtactactt gtcaaaatta
    37021 gttagcatac cttatttagt gaattttcta agcattgagg atctgttggc agacgatttt
    37081 aagtacatcg gctttgtaaa ggaaatcaac agtgccagta ttctgtatgc agctgatagt
    37141 aagctgatta gtgaagttca aagtcataga gagattactt ttagcaactg gaaagtaatt
    37201 catgcgattg atagtgattt gagaaatctc ataaatgaat ataattgggt tattgataat
    37261 ttcagcacca atgacagtat atttcttaat tatgatgatt tgcatgttac ctcgaacaat
    37321 agttaccttc gttttttgga agataaggat aagagtttgg tgctgtttgg agcatctgag
    37381 tttgggctat atgcacagga cagcttgagg atgaataata ttatggttga ttatttttgc
    37441 gataatgacg taagcaaatg tggcagcatt atcagtggtg cggccataat atctccggat
    37501 caattattgg cgctttatgg aagggtcaat atattgatta ccagtatgta cagggcggaa
    37561 attgaaaggc aattgcttca tttagggttt agtgaaagtg atatatattt tatacctcgt
    37621 gagatatatg gcagattatc atattataag aaaagtcagg aggcaatttc ctgcagttgt
    37681 gaacgaattg ctgaagctct agatatcttg gaggatatta aatcaaagca ggtattcata
    37741 aatatcataa aacacaagat gtcctttgac tttaatttga taaatgagat tatcgaccat
    37801 gaacagtact ttgatagcag catcctggag ttctgtgacc atgaaacctt tgtagatgca
    37861 ggagcctacg acgggcaaac tacaatggag tttattgcga aggtcaaggg ccgcttcaat
    37921 aaagctttcg tttttgaacc tgataagaat atgcttgata aggtggccgg caccattgat
    37981 tcagtaaata aagacaacaa tatcaaactg atagccggtg gagtatatga caaaagcggt
    38041 gtcattaaat ttacaccagg agaaattggt ggttcgcata taagcaataa tggttcggag
    38101 aatattgaaa tttataagct ggatgacgta ttaaaagatg aagatgtaac atttattaaa
    38161 atggatatcg aaggtgcaga aatgaatgca ttgataggtg cacagaatat tatccgggaa
    38221 aacaagccca agttggctat atgtacctat catatgcctc cgcaggattt ctgggagatt
    38281 ccattatata taaagacttt ggtgcctgaa tataagatat ttataaggca ttatggttat
    38341 gatgtgcgtg ataccatatg ctatgcggtg atatagaaaa attaacgagt gaggggatat
    38401 atatgtctaa gaatcctttt gtatctgttg ttattaataa ttataactat tcggaattta
    38461 tatctgaagc tatagaatca gtgttgaagc agacatataa gaatttcgaa ttgataattg
    38521 tagatgatgg ttccactgat aattcagtga gcatcattga gcaatatcat aagcagaatc
    38581 agagaattat aactcctgta tataagaaaa acggagggca ggcttccgct atgaataccg
    38641 gatacaacat ggcaggagga gaaataatcg ctttgttgga tagcgatgac tattggtttc
    38701 cggaaaagct tgaaaggata gtaaagtgcc atgaaaacta tgatattgtt cagcacaatc
    38761 tattgataaa tggagaacag aagtatatca acttaagaaa cgatgttgat tggagcactg
    38821 cattaaaaaa gtatggttat cttttaggat actgggtccc gacatcaggg ctgtcctttt
    38881 caaaagctct tttgggaaaa gtatttccta ttcctgaggt ctcattaagg ctatgtgccg
    38941 atgcttatat tatatggaat gcactatatt actccaaaat tttttctata gatgaagctt
    39001 tgggttttta cagagtacac ggtaataata attggcataa taagttcaat gatgagagaa
    39061 ttaagaatac tatagacacg atactggata ttttgaatga aaagctcata ctatcgggag
    39121 aaagtgagac aatccaatat aatagtacat tatataagga tttacttggc ctttttattg
    39181 agaaagataa aaaatacgta ctgtatggta ccggatcact gagtgaagag tttacaagga
    39241 tagtagaaca atgcgaaagt gaaattgtat actattctga ttctaattcc gataaatggg
    39301 gaaagcactt tatgggcaga gagattatac cccctgaaga cctgttgtca aaaagagagc
    39361 attttgacaa aatcgttatt gccagcagtt ttatagatga gattttaatt gtgctggaga
    39421 agttaggctt tgtaaaagct tcagatatta tagtgccaca attatagcag ctgtgggaat
    39481 gacgccaata tttaatgtgt agcggaggat taatatatga atatctgtgt tattgggaca
    39541 ggctatgtgg gattggtgac aggcacttgc tttgcagaga tggggaacaa ggtatggtgt
    39601 gttgatgttg acaatgctaa aattgaaatg cttaataaag gtatttcccc aatatatgag
    39661 cctggtttga aagaaatgat tgaaagaaat tcaaaaaacg gcagactaaa gtttacaacc
    39721 gacataaaag aagggataga gtcttcgctg tattgcttta ttgcagtcgg tactccgccg
    39781 ggtgaagacg gttcatctga tattacaaat gtgcttaagg ttgccggcgc aataggccaa
    39841 aatatgaaca agtatactat tattataaat aaatctacgg tacctgtcgg tacaggagaa
    39901 aagactaaag aactagtaag agaagagtta aggttaagga atgaattgaa tattgatttt
    39961 gacgtggttt ccaatccgga gttcttgaaa gagggagcag ctatagaaga ttttatgaga
    40021 ccggatagga taataatagg aacagataat cccagaaccg cagaattaat gaagcagcta
    40081 tatgagccat ttgtgaggaa tcagcatcca atattaataa tggatattaa atctgcggaa
    40141 ctaaccaaat atgcggcaaa ttcaatgctg gcagcaagga taagctttat gaatgaaatt
    40201 gcacgcttat gtgatattac gggtgctgat gtaaataatg tacgtgttgg catgggtatg
    40261 gacagccgta taggaatggc ttttttgtat gcgggtacag gctatggagg ctcatgcttt
    40321 cccaaggata ttaaggaact gataagcttg ggaagcagaa acggactcga aatgaaaatt
    40381 gcaaaagctg ttgaagaagt gaatgagtca cagaagcact acttggttaa tatggtaaca
    40441 aaaaggttcg gatgtgattt gaaggacatg agcttcggca tatggggact tgcttttaaa
    40501 cctcagacag atgatatgag agaggcgcca tccctggtta ttatgaagga actgaccggt
    40561 atgggagcca atataatagc gtatgatccg gaggcaatcg agcagtcaaa gtgtattatg
    40621 agtgaagatg aatataatat taaatatgtt tatgatatga tggaagccat agataaggtg
    40681 gatgccttga tattagttac agagtggaga cagttcagac aaccggattt tagagaaatg
    40741 aaatccagaa tgaagcagtc aatagtattt gacggaagaa atcaatatga cacaatagca
    40801 atgcaagaaa atggctggga atattattgt atagggagaa ataccaatgt caaataagag
    40861 gattttagtt accggaggcg caggctttat aggttcacat ttgtgtgaaa gacttctgaa
    40921 agatggcaat catgtaatat gcctggataa cttttttaca ggatctaaaa ggaatattgc
    40981 tcagcttatg aatagtaagg actttgagct gattacaggt gatgtggaga atcctattga
    41041 catagaagca gatgaaatct ataatctggc ttgccctgca tcgccggtgc attaccagta
    41101 tgacaaagta aaaactttga aaacgaatgt tataggtgcg atgaatatgc tggagctggc
    41161 aaaaaagtat gatgcaaaga tattgcaggc ttcaaccagt gaggtctacg gagacccaat
    41221 gattgatata caggatgaag gctatagagg caatgtaaat cctataggaa taagagcctg
    41281 ttatgacgaa ggcaaaagat gtgccgaaac gctgtttttt gattatatgc gtcagtataa
    41341 tgtagatata aaagtagtaa gaatatttaa tacctatgga cccaaaatgc atccggaaga
    41401 cggcagagtg gtcagcaatt tcatagtgca agcactgaaa ggcgagaata tctccatata
    41461 tggtgatgga agtcagacca ggtccttttg ctatgttgat gacctgcttg aaggattgat
    41521 atcaatgatg aacacatcaa aggatatcaa cggccctatt aacttgggaa atccggagga
    41581 gtacaatatg ctggagctgg ctgaaaaggt aataaagctg acaggctcgt cttcaaagct
    41641 aatttttaag gaattacccg aagatgaccc caaaaagaga aagccggata taagcttagc
    41701 agaatcggtt ctcggatgga agcccagcat tgcagtagaa gacggattga taagaacgat
    41761 aggctatttt agtgaattaa ttcgctgaca tatcgagagt aacgaaacga agggcattag
    41821 ttgaaagcta acgaggagca ttacagatga aaaagagtat aatactgttt ggtgcttcaa
    41881 aatccgggga agcagccatg aaaaaatata caaaagaata caagattgct tatttctgtg
    41941 ataatgataa aaacaagtgg ggaacaagct tccgaggtgt tgaggtggtt tctcccgaca
    42001 atctgaagat gctggacttt gacaagatca ttataacaag catgtattat gatgaaatat
    42061 caaagcagct cagtaatatg gggatagaaa aatacgaagt ttttaagttg agtgcaaagg
    42121 agagaagcaa taaaagcctt agagaatata taacatcaat gcattgcaaa atacaatata
    42181 tatatgaggt aagccgctct gcatattggg atgagcaaca gagaaaatgg aagcagaagc
    42241 tggattttaa taaaatggct gaagaactgt caaaacagct tcgtgctgcc ggtttagaca
    42301 aacctgtgtt atatgatgtg agtattaaaa atgatattct taaagaaagg cctaagatta
    42361 ttcatgctat accgaacacc tttgttggcg gttcaaccca gcttattgtt gatcttatag
    42421 agcatttagg ccataaatat gacatggaga taatcactgc agcattacct aaggagggta
    42481 tgcacaaagg catgactata catgactttt ccggagccaa atccggtgaa gaagtatctg
    42541 agttcttaaa aagtaaaaat gcagaccttc tgcatattca ttactgggga gatacctggt
    42601 acgagcatat ttttgattcc ttgaatacgt atgactgcaa ggttgtggaa aatatcaaca
    42661 cgcctattgc tgcatatgcg cataataaag tagacgaata tgtgtatgta agtgaatatg
    42721 ccagaaggat gtttggggat tttcctgaga attcatctgt aatacatccc ggcattgacc
    42781 tttcccattt tgaacccagt aaagacaata aaaggccgta tgcggttggt atggtataca
    42841 ggctggagca ggataagctc agggaagact ctataaaagt gtttattgag ttagctaaac
    42901 gaaagccgga gctgcttata tacatagttg ggtatggtac ctttttcaat tcatatgtaa
    42961 ggcaggtata taaggaaaat gtaagagaga attttgtctt tgcggggtat gtcccttata
    43021 aagaacttcc ggccatatat gacaagtttt ccatattcgt agcacctgta tggaaggaaa
    43081 gcttcggaca ggtaacaccc tttgccatga ataaagagat ggtagttacg ggattcaaca
    43141 ttggcgcttt gccggaaata cttgacggag aagtgactct tggaaacagt ctggaagaat
    43201 tggttgaaag gacaatttac tatcttgata atcaggaggc ttatagagca aaaggcagcg
    43261 agctgcggag aagagctcag gactgcttct gcgtaaaggc catggtttca aaatacgagc
    43321 agatatacga taagcttctg tctatgtaaa ttatcaatac aaggagttgc tgaatgcaga
    43381 aatgctttaa cagctttctt ttttaatatg ggggcgcaca gcgatacagt ctgttaaaga
    43441 aatgttgtaa aaattccgat gtattattag gattctaatg atagaagatg ttatacagta
    43501 ggagggctca tgaatatact ggttattggc ggacagggtt tcatagggca taacctggtc
    43561 ctgaagctcg tttctgaagg ccataaggtt acagtgtttg agaagaattt taaaccggaa
    43621 agaaaattgg attcctgcga ctatacaatc ggcagttttt ctgagattgc tcaatatgag
    43681 aatgtccttg aaggaatgga tattgtttat catttgatta gtacgaccat tccaaagact
    43741 tcaatggata atccttcatt tgacatacag tcgaatgttg tggatactat taaattatta
    43801 gagctttgct gtaagcataa agtaaggaag ataatatttc cttcctctgg cggcacaata
    43861 tatggattag cagagactat tccgattagt gaagatcatc ctaccaatcc gatttgctct
    43921 tatgggataa caaagcttag tatagagaaa tatctgcata tgtttaatca tttatatggg
    43981 cttgattatc aagtgtttag aatatccaac ccttatggtc cgtttcaaaa ccccttttca
    44041 aatcaaggag cggtcggtgt ttttctgggt aaaatactga ggggagaaga aatagaaatt
    44101 tggggtgatg gcagtgtatg ccgtgactat atatttataa gtgatgtagt acaggctctg
    44161 tacctcgctt ctgagatgca tacagatatc aaggttctga atattggaag cggtaaaggt
    44221 gtgaccttga acgatttggt agatattata agcagagtta ccagcaagga agcaaaggta
    44281 gtaaataagg aatcaaggga aattgacctt ccggtaaatg tacttgacat ttcaaaggct
    44341 gcagataaat tgatgtggtt tccgcagatt ccattggaga agggcataga aataacctgg
    44401 gattggctta tgaggtataa ataatgaaca tattgcacat aacaactttt ttgcagggtg
    44461 gtgcaggcag aatcatatgt gatcttgcga ttgaacagaa aaagctggga cataatgtat
    44521 atgtcgtaac aagcaagacc caggaaagcg actattgcaa ttatgcggaa tatataaaca
    44581 ggcttaataa tgcagatgtt atattatatg aaatagactc aacctttaaa agagacttgt
    44641 atcttaattt aaaggttgtt gagaaagtaa aagaaataat aattaacagt gatatcgatt
    44701 taatacatgc acatgcggca atacctgcat tggtaggtct tatatccaga caagcatcac
    44761 acaagtatat acctgtaata cactccatgc agggctgggg aacaaataag accccggaac
    44821 aggaggaaac ggacaaaatc ataatgaacg gacttgatgc aataattgcc tcatcacaaa
    44881 gctcctttga cttaatgtgt gataaaggta ttaataaaga gaaaataggt ttgatatata
    44941 acggaattgc atgcacagat attagtgaat ctgataaagt agatgatgat gtcagggaaa
    45001 tgattaaact aaagcatgag ggagtaaaaa ccataggctg cataggaaca atttgcaaga
    45061 gaaagaatca gggacttctt cttgaagcat tcaaagaggt agtgaaggca gaggatgcaa
    45121 tctgcgtgtt cgtaggcgag ggtgagttgg tacacgaact gatgcagaaa gctaatgaat
    45181 atggtatagc agatagagtt aggttttggg gatataagga aaactcatat caattttata
    45241 aatactttta ttgcgttgtc ctgccttctt taagtgaagg acacccaatt acgactgttg
    45301 agtgctttag agaaaaggtg ctattcatag gcagcgatat accgagtatt acagaattga
    45361 taaagcacgg ggagacaggc ttcttgttca agtccaataa cccggcttca ctggcaggtg
    45421 taattaagca ggttttatcg cttaccgatg acgaaagtgc tgttatagca gaaaaagcct
    45481 atgattttta cagcagcagc tttactctta atgcaatgct tgataattac atgcgcttat
    45541 atgaagcttt gatactaaag ggcaaaggtc tccataaata attataatca tatccaatca
    45601 agggaggtat tatatgaaag gcattatcct tgcaggtggg acaggctctc gtctttaccc
    45661 cctgactaaa gtaaccaaca agcatctgct gccggtgggc aagcatccta tgattgacca
    45721 tctgattgca aagatcaagc aggcaggaat atgcgatata atggtcatta caggtaagga
    45781 gcatatgggt gcggtagtaa atctattggg aagcggacat gattatggag tgaaattcac
    45841 atacaggata caggatcagc caggagggat agctcaggcc ctcggcctgt gtgaggaatt
    45901 cgtgcacgat gacagatgtc ttgtaatact gggagacaac atatttgagg atgatatttc
    45961 ggaatatgtg gaaaacttta tgcagcagga gaagggtgca aaaatattga ttaagaaagt
    46021 gcccgaccct cagcggtatg gagtggcaga actgaagggc gggaatatcg tcggaattga
    46081 agaaaaaccc gaaaagccaa agagtgattg ctgtgttacc gggatatata tgtacgacaa
    46141 tagagtattc aacgtaatta agtccttgaa gccttctaac aggggagagc tggagattac
    46201 agacgtgaac aactggtata taaaggacgg ctcccttacc tttgatgtgc ttgagggctg
    46261 gtggacggat gccggtacct tcagctcctt tgcttatgcc aatgagctgg cgaaggataa
    46321 ggatttgagc ggaatacttt gcagggatta tgagatattg agggaagccg ccataacaaa
    46381 agaatgatga cataggggag aaatggtatg aaaatgctga ttactggcgc agcagggcag
    46441 ttgggaagtg aactcctcag gatagcgaag gagatgaact gctctttagg tgcaattgat
    46501 aggtcttata ataatcctga tattatggca cttggtgcta aggagctgga tattgcagac
    46561 ctgcctgcgg tcaggaatat agtgggcagg ttcaggcctg atgttattat aaatacagca
    46621 gcgtatacca atgttgacgg ctgtgagaaa gaagcggaag ctgcgttcaa agtaaacgca
    46681 cttggagcaa ggaatgttgc tatagctgcc caagagacaa attcaaagct tatacatatt
    46741 tctaccgatt atgtttttcc cggtggcggg agtactccat acagggaata tgacaaaact
    46801 tcaccaaaaa gtatttatgg caagactaag ctcttaggcg aggagtatgt aagagagttt
    46861 tgcaaaaaat attttattat tcgtacttca tggttatatg gaagaaatgg tcggaacttc
    46921 gtccggacta tcctcagtgc agctaaagag aagcagtatc tcgaagttgt ggatgaccag
    46981 aagggatgcc ctacaaatgc agaggattta gcataccaca ttttaaaaat agcagtgact
    47041 gacgagtatg gcgtttacca ctgcagcggc aatggagaat gcagctggta cgagtttgca
    47101 atcaagatat tggagattgc aggaattgat tgccctgtga agcctattaa gacgaaccaa
    47161 ctagacagag cagcagagcg ccctgcatat tcagtaatgg ataatatgat gctgagactg
    47221 acggtgggag atgacatgcg gagctgggag gctgccattg agcattttat aaaggggctg
    47281 ctgatatgaa aacatatttg attacaggtg gagccggctt tataggttcc aatttcatac
    47341 aatatatgtt gaataaatat agtgacattt acatcatcaa ttatgacaag ttgacttatg
    47401 ccggaaatcc ggaaaatctg aaaagtattg aaaaggattc aaggtataat tttgtacaag
    47461 gagatatctg cgataaggaa aagttaagtg agctgtttga taaacatgat attgactatg
    47521 ttgtgaactt tgctgcggaa tcccatgtgg acagaagtat aaaagaccct gagatattta
    47581 taaggactaa tgtgctggga acagtcaact tacttaatgg tgcaaaagca gattggtcag
    47641 agggacaggg cttcagaaga gggaaaaagt tccttcaggt gtctactgat gaagtatatg
    47701 gctctctggg ggatacggga tatttcacgg aaaatacacc tctgaatccc catagcccgt
    47761 actcatccag caaagccggc gccgaccttg tggtaaaggc ttattatgat actttcggaa
    47821 tgccgataaa cataaccaga tgctcgaata attacggacc ttaccagttc cctgaaaagc
    47881 tcattcctct gattatcaac aactgtctga acaaaaagtc tttgcctgta tatggagacg
    47941 ggcttaatgt aagagactgg ctttatgtgg aggatcactg caaggcaata gataacgtga
    48001 taaaatccgg ggaaccggga gaggtgtata acattggagg cagcaacgaa aggacaaaca
    48061 tacatatagt taagtatata ataaattatt tgcatgataa tgtcgataag gaaatcgatg
    48121 aaggactgat caaatatgta gaggacagga agggacatga cagacgttat ggaattgatg
    48181 caacaaaaat aggggaagaa ttgggatggg ctcctgaaac aggctttgag gatgggattg
    48241 taaagacaat agaatggtat ttggataaca tcgaatggat gagcaatatt acttcaggag
    48301 aatatcgcaa gtactacagc tcaatgtatg aaaatgcctg agatgtattg aaaagtgagg
    48361 tatcataatt gggacggttt atatttagca aaactcatat tagagggctt tatattattg
    48421 aacctgctgt atttgaagac ggcaggggat atttcatgga aacgtataat tacaatgatt
    48481 ttaaggaagc agggctggat atgatatttg tacaggacaa tcagtccagt tccgggaaag
    48541 gtgtattgag agggcttcat ctgcagaagg aacgtccaca gggcaagctg gtgagagtat
    48601 tgcacggcga agtatttgat gcagcagtag atctgagagc cggctcagac acctttggaa
    48661 gttgttatgg tactgtatta agcggggaaa acaggaaaca gctttatatt cccgaaggct
    48721 tcgcccatgg tttcctggta ttgtcggata cggcggaatt tgcttataag tgtactgatt
    48781 tttattatcc cgaatatgag aaaggcatta tatggaatga tcctgatata gccatagact
    48841 ggcctactga taaagttgaa ggtataatac tgtcagataa ggacaagagg cttcctttgc
    48901 tcaaaagtat attggacagc tttgaaccaa aacagtaagg cgaggtggtg ctatgaagct
    48961 gcttattacc gccgtaggca agcgtgtaca actaataaaa tacttaaaaa gacaatttac
    49021 cgtgataggt gcagactgct cgatgcttgc gcctgcatca tttcatgttg accgtttttg
    49081 tccggtatca ccttgtgata atcccggata tattggggaa gtactcaata tttgcaggaa
    49141 atatggaata gacattttac tgcctcttta tgaaaaggaa ttttacttat tggattctat
    49201 cagggaagag cttttgaatg aaggaaccct tttgttgttg tccgataaac gtgtattgga
    49261 tatatgcagc gacaaatggg agacatatca gttttttatc ggaagcaata tcagtacgcc
    49321 tataagcttc aagaacaagg cagagtgcaa tattgaattt ccgatgttca taaaacccag
    49381 aaggggtatg ggcagcttca attcatatga attaaatgat atggaggaat ttgattttta
    49441 ctatgaacgt atacctgaac ccatagtgca ggaaaaactg tcgggggttg aatataccat
    49501 ggactgctta tcggatttag gaggcaaagt tatatccgtt gtaccaagag agcggctgga
    49561 ggtaagggca ggagaggtta ccaaaacacg aacggttaaa gatatggaaa taatagaaaa
    49621 gaccaggtat ttactggagt gccttggcag cgcaggcccg actacagttc agtgtttcag
    49681 aacggttgaa ggggatatta aattcactga agtcaatgtc agggttgggg gcggtgttcc
    49741 gctcacaatg gaagccggaa ttgattatgg aaagatattc attgacttga ttgaaggcaa
    49801 caagccggaa atactgcttg gaaactttaa agagcttaca atgctcagat atgatgaggc
    49861 ggtttttata tgaaggcatt agtttttgat ttggatgaca ccctttataa tgaagcagat
    49921 ttcgtgaatg gtgcctttat ggaagttgcg caataccttt caaaaaagca ttgcatcttt
    49981 agtcatgagc tatatcatgc catgttaaag ctgcttcaag aaaagggcag gggcagaata
    50041 tttaatgata tctgtgattt atacaacttg gatgaagata taaataatct tataaagata
    50101 tacagaaatg cagttccacc aatctcccta tatgaagatg cggagtattt tttgaaatac
    50161 tgccaaggga agtacaagac aggcttgatt acagacgggc tatattatgt gcagcggaac
    50221 aagatacggc tgctcggtat tgagaaatat ttcgattcta ttattgtaac tgatgaacac
    50281 ggggagggtt tctgcaagcc ggacatcaaa ccatatttaa aaatggctga aaacttagga
    50341 gttgcgctcg acagtatgat atatgtagga gacaatcccc ggaaggattt ctgcggggca
    50401 aggcaattgg gagtatatac ggtcaggatt ataagaccgg ccggggatca catgggatta
    50461 agattgggca gtgattatga agcggataga gagataacaa atatgtatga gctaaaagcg
    50521 gtgatagata cgttgaaata aaatccggag gattattatg gaacatagat atgaaaaaca
    50581 ttgggaacac aaagaataca aaattattga gtgcatggat tgcggcttta agcatctata
    50641 ccccgtacct gaaaaagaaa gtatgaagga gttctatgat gaaaagtatt attcagagat
    50701 aaaacccttt tattataaaa atgtgacgga ggacttcatc ctaaggaaat ctgaagaagt
    50761 tgacaacaat aaaatatatg gtgcaatata tgacaaagta ggcgaatata aaagggcata
    50821 tgaaaacgta atggttgata tcggcagcgg aaatgatttg ctggccttat attttcagaa
    50881 taaggagtgg gaggtctgcg tagtagaacc caatattaag gctgcagaat atcttaatag
    50941 ctataacctc aatgtaataa atgactatat ggaaaatatc aaaccggaaa ttttaaaagg
    51001 ttcttcattt ataaatgtac aatttgtcct ggaacatatt cataaccctg cagcattcct
    51061 tgagaaagca tatagaatgc tggtgccggg aggtataatc aggatttgtg tacctaatga
    51121 tttcagtgag gggcaaatgg cattcaaaga ataccatagc gaggcatata gatggatagt
    51181 gctgcctgac catataaatt actttaacat taattcgctt agcggcctgc tttcaaggac
    51241 aggcttcaga gaattgtaca ggacaacgga tttcccattg gaaatactgc ttatgggagg
    51301 catgaattac tacacggagg aagaatataa aaagcaagtg gggccattta tatataattt
    51361 tgagaattca ctgaaaagta cagggcgaaa gcaattattg gacagatttt atgaagggct
    51421 tgcaaatatt gggatgggaa gatcaattta tatgtatgca attaaggaat aattaagaat
    51481 gcggtgatag gatggttaca tgtatagtac aagcccgaat gggatcggaa aggcttccgg
    51541 gaaaggtgct caggaaggtt ctgggtatcc ccctgatatt gttgaatctg aagcggctgg
    51601 aaaaatcaag ggaaattgac aggatgatac tggcaacctc cgacaggcag ttagacgatc
    51661 ctttgtatga aacagtcaaa ggagaaggct ttgaggtttt caggggagat gaagagaatg
    51721 tccttaagag atacagggac tgtgttctgg aatatggcgg agatattgtc gtaagggtga
    51781 cgggggattg cccccttatt gatcctgata tagccgacaa tgtaatatcc cattataaga
    51841 tgtacgatta tgattatgtc agattggatg taccggatac atttgtcaga ggctttgata
    51901 cggaagtgtt ttcaagggaa gctctgttta aggcctttga tttggcggac gagtacaggt
    51961 ataaggaaca cgttaccgcc tatatgtacc ttcatccgga gctgttccgt acaggacttg
    52021 tccgaggaga aagtttgttc aaaaagaact ataggctgtg tgtggatact ttagaggatt
    52081 tcacattggt tgagaagata tatgagcatt ttggagacat atatgtatct gccggggatg
    52141 tggtaaaatt tttagacaat aaccctgata ttgcagccat aaacagtgaa gttgtgcaaa
    52201 agcatgttta aggagtccgg atatgaatga aattgttctg aaaaacggaa gaagaatagg
    52261 tgaagataat ccttgctata taatgacgga catagctgcc aatcataatg gggatttgga
    52321 aacagctaaa gagctgataa ggaaagcagc tgaagccggc gcagatgcag taaagtttca
    52381 gacttatagg gcagaggatt tatattctaa gaaaactcct cagttttcaa gggattccat
    52441 gaagccttat gatttgatta aaaaggttca gcatcccagg gaatggatac cgatactgtc
    52501 cgaacatgct aaaggcttcg gaatagactt cctatcctcg ccctttgatt atgatgcggt
    52561 agatttgctt gacaatacag gtgtcagtct tttcaaagta gcatctcttg aaatagttga
    52621 tttaaaactg ataaaatata tagccggtaa aggaaagcct gtggttttgt caacaggtat
    52681 ggcgagcctt ggagaaattg aagaggctat agaagctgcg agaagtacag gaaatggaga
    52741 tattgcttta ctgcactgca atacctgtta tccggcaccg gcacacattg ttaatctgaa
    52801 agcaatggaa acacttcgaa acaccttccg gcttcccaca ggcttttcag atcacacatt
    52861 aggctggcat attcctgtgg cagcagtggc tttgggtgcc tgcataatag aaaagcattt
    52921 tactttaagc agagagcagg aaggtcccga tcatggtttt tcaattgaac cggaggaact
    52981 gaagctgatg gtgcggcaaa tcagggatgt ggaaaaggct atgggggacg gagttaaaaa
    53041 ggtttcgcgg gaagaactgg agaactacga aaagggaaga agaagtctga tagccataac
    53101 tgatattcca aaaggtacga aaataacaga aagtatgata accgcaaaaa gaccgggcta
    53161 tggtataaag cctaaatatt ttgatatggt aattggcaga actgcaaagg ttgatatatt
    53221 cgaagatgat gtattgctgt gggatatgct ggtatagtat agaaattcag gagatgggct
    53281 tatgaagata gtaatcaggg cagagggagg tcccgggatc ggcatgggtc acgtaatgcg
    53341 tatgctggtt ttggcggata agctgaaagc ctttgcagag gtggtttttg cctgcaggga
    53401 taacaaggag tttgaaccgg gcgtaaggca aattgaagct tgcggctatt cagtatggag
    53461 gttcagcgga gagacggtca ttgacggatt ggctgcaata ggcggggatt gcctgattac
    53521 agacagttat gatatagatg aaggctattt tgataggact aaagatattt ttggcataac
    53581 cggatatatg gatgatataa ataaagtccg gatttatgcc gattttatta tcaatcagaa
    53641 tatttatgcc gaggatatgg aatacaaagc cggtattggc accaagttat ttttgggtac
    53701 acggtatttg cttctgagag atgaattcag gaacctgccc aagagaaaaa caagaatgca
    53761 tatagaagac gtattgatta cattgggagg ggcggatccc gggaacctgt cggaaactat
    53821 tgcattaaag ctcagtaagg tttttcctga aatcacattt catatagcgg tgggaccatc
    53881 ttttactaat agggatagta ttgagagaat agcgggagga aatatcattc tccactataa
    53941 tcccaagatg tcagagctta tgctgaaatg tgatgctgcc atttccgcat gcggaagtac
    54001 cgtctatgag ctttgtgcct gtgggacacc tattatcgga gtcgctgcgg cacctaacca
    54061 gattatgacg tcacgcagca tggatattaa aggtgcatta aaatatgcgg caggtgccgg
    54121 agaaattgtt cagcaactgg aaagcttgaa ttataataca agggcaagaa tgtcggatac
    54181 agcccgggaa ttagtggatg gttacggaag tataagggta tcggagcaga tatggaaaat
    54241 catctgtgag aaggaaaagg gaggtactgt atgaggaagt tcatacccta cgggagacag
    54301 tatgttgatg aagatgacat acaggcggtg gttgaagttc tcaggagcga ttttttaacc
    54361 acagggccca aggttgacga acttgaaaat aaaataacgg agtatacagg ggcaaaatat
    54421 gctgcagtat tatccaacgg aaccgctgct cttcatgccg cttgctttgc cgcaggcatc
    54481 ggacccggag atgaagtaat cacttcaccc atgacatttg cagccacagc taactgcatt
    54541 ttgtatatgg gagggaaacc ggtctttgca gacatcgatc ccatgaccta taatattgac
    54601 ccttgggata ttgaaaagaa aatcacccat aggacaaagg caataattcc ggtgcacttt
    54661 accgggcagc cctgcgaaat ggacgagatt ataagaatcg cggataatca cggattaact
    54721 gtcatagaag acggtgctca tgccatggga gcggaataca aaggcagaaa aatcggtact
    54781 ataggaacca tgacaacctt cagcttccac cctgtcaagc atattactac aggtgagggc
    54841 ggagctatta caaccggtga tgcatcctta tatgaaaagc taactttgtt tagaactcac
    54901 ggtataacaa aaaatgcgga aaaaatgact gactatgatg gactttggtt ttgtgagcaa
    54961 cagtacttag gctttaatta cagaatgact gatatacaag cagcccttgg agttagccaa
    55021 atgaagaagc ttgattcatt tctggagaaa agaagaagct atgcagctct atacaataaa
    55081 ttgcttggta ctattgatgg attaacaatt ccataccaaa atgaatatgc aagctctgcg
    55141 tggcatttat atataataaa gctaaacctg agcaagcttg acattggaag aagagaagtt
    55201 tttgacaagt taagggatag gaatataggc gtacaagtac attatattcc agtatattat
    55261 catccctatt acaaaaacat gggatatgaa aaagggcttt gtcccgaagc agaagccctt
    55321 tatgaaggaa taataacact gccattatat ccaacaatgg aaattgaaga tatatattat
    55381 atagctgaca atcttaggga tatattaagg agttgatata cattgaacat gcttgttaca
    55441 ggcggagcag gcttcatagg acgctggaca gtgaaaaggc tgcttgaagg cggccacaag
    55501 gtttggatac tggatgacct gtcaaatggc tcattggata acataaagga atttgaagga
    55561 cataaagccc tgatgggatt tttaaaaggt gatatcaagg ataacagcat attggaaaga
    55621 ctgtttgaaa acaactttga tatatgcttt caccttgctg caagcataaa tgtccaggac
    55681 agcatcgacg atccccggac aacctttgag aatgacgttg cagggacctt taatgtattg
    55741 gagcaatgca ggaaacataa tgttaaaatg gttttcatga gcacctgcat ggtatatgac
    55801 atggcggaaa atgatcaggg cataaacgag cttcatcctg taaaaccagc atccccatat
    55861 gccggaagca agatagccgg tgagagcctt gcactgtcct actggcatgc ttataagctt
    55921 ccggtagtgg tagtgagacc ctttaacaca tatggaccct ttcagaagtc cggcggagaa
    55981 gggggagttg tatcaatatt tattgaaaga aagcttaagg gtcaagcttt gaatatatac
    56041 ggcgacggga agcagacaag ggacttgctt tacgtggagg attgcgcgga gtttctggta
    56101 atggcaggct gctcggaaaa atcaaatggg gaaattataa atgcaggatc aggaaaagat
    56161 ttgagcataa atactttggc atatatgatt tcgggtaatt atgagcaggt gaggcatgtt
    56221 acacatatac atccccaaag tgaaatacaa agactgctct gcaattatga aaaggcagag
    56281 aagcttttga attggagccc caggaccgga cttcaggaag gaatagaaaa aactgaagcc
    56341 tggctagaaa ataatactat taagttataa aaaagctaaa gaaattcgtg ttttttatcg
    56401 atatatacta tatataacat attgtaagga tgcgggaaat atatccaagg agggaagtaa
    56461 tatgaaaata gcaggaaatt ctataagccc tgctgtaagc tcagatacta tgagaaacaa
    56521 tgcagttaaa gggaatcatg ctgaaataag taatagaaat aatgcagtaa agagcggtga
    56581 acaggaagtt tccgcaaaac ctaaagataa gtcagaaata aacgagtatc agtttataag
    56641 agcggttgaa acagcaaaca aagcattgga aggttcattt acaagctttg aaatctctgt
    56701 acatgagaag acaaatagga taatggtaaa agtagtggac aaagagacta aagaagttat
    56761 tagagaaatt ccaccggaaa aaatacttaa tttgatggct aaattatggg aaatggccgg
    56821 tattattgtg gacgagagga ggtaggcaac atggcttcca atattaatag tgtttcaaca
    56881 ttaaggttct caggaattgc atcgggactt gatgtagacg atatggttga acagcttatg
    56941 aaggttgaaa gatcgaagat ggataaagtc aagcaggaca agcaattatt ggaatggaaa
    57001 agggatgact acagaagtat taccagtctt atgagaagct ttcagagcag ctattttgat
    57061 gtaatttcat caaccaatat gaggtcttcg acaagctaca aggcattttc aactactgtc
    57121 agccctagtg atgctgcgga tgcaggtgcg gttagtgcgg tggcaacatc tgctgcttct
    57181 gagggcacac attcaattat agtaggaaat cttgcaacag cacaggtaag acaaagtgca
    57241 ggtggagtta ctaaggatgt acagggcgat tcaggatata cattgacagc gggagaaacc
    57301 tttaagctga caattgatgg agttaccaag tcaataactt tatctgacct ggatgggact
    57361 gacggagtaa cactggctga cttgaacaag tccatagaga atgcctttgg cagtggaaaa
    57421 gtaacagtag atgatacaac aaatccggga aagcttacat tcaaagtctc cagcgaaaca
    57481 aacggtataa ataaaataac agtcagtgct ggtactacaa ataatgcttt ggccaatatg
    57541 ggttttggag ctgatgcaat atattctaac agactgagca gtggtgacac acttgcagtt
    57601 ttaaagtcca agctggatga tagcaatgga ggattggcat ttacagcttt atcggatggc
    57661 acagagggaa taaagcttgc tataaatggt aaaacctttg agtttagcga aacaacgact
    57721 ttatatagca tgatgaatca aataaaccgg gattcaactg caaatgtaaa tatgcagtat
    57781 gacgaaataa atgacaaatt caagtttact gcaaaacaga ccggcgcagg gaacaatatt
    57841 gaaatcagcg aaagtggaag cagctttatt accactgcag atatcagtgt caacaatgcc
    57901 ggtgaagacg caaagatcac aattgatggt actgaaatta caagaagcag taatactttc
    57961 actgcagatg gaataactta tactgcactt aaggaaacag ggacaagaga agtgacagtt
    58021 tctaccaaac aggatgttga tacggtatat aataagatta agagctttgt agacaagtat
    58081 aatgaactga tatcaaaaat taatggtgag cttacggaag aaagagaccg ggattatgct
    58141 cctcttaccg acgaccagaa agaggatatg actgaagatg agataaaaag gtgggaagag
    58201 aaggcaaagt ctggtaccct tcgcggagac agcttgttgc agaagatagt aagtgattta
    58261 aggaacactt tgtatgccgg tgttgaaggc gaatccggaa catcattttt attcagcata
    58321 gggatcgaaa caggcagctg gaacaacaag gggaagcttg taattaacga gagtaaattg
    58381 aaggctgcaa taaaagattc accggacaag gtagcagaaa tattttctaa agaatcaagc
    58441 atcgcatact cggcagatat gacttcaaca gaaagaagca ctcgttttaa agagaatggt
    58501 attattaaca ggatgtatga tgtcattcag gataatataa ggacatattc caataaaaac
    58561 gggcagaagg gtgctttgct tgaaaaggcc ggtgtggtag gagaaacgac ggaaaataag
    58621 aatattatgt acagcatgat aaaaactcaa agcgatatga taacgacctt gactaaaaaa
    58681 ttacaaacta gagaagacta ttattataaa cagtttactg ctatggaaaa agccatatct
    58741 caaatgaaca tacagtcagt atggctttca cagcagtacg atagtcaata atacctggag
    58801 gaagaacaaa atggcaatgg ctaacaatgc acatgatata tataaaaaga atatggtaac
    58861 tactgcaacg ccgcaagaat tgacacttat gctgtataat ggcttgatta ggtttcttaa
    58921 acaagccgtt atgggtattg atgaaaaaag catagaaaag gctaataata atatcattaa
    58981 agctcaggac attttatttg agttcatgag gacccttgat atgagctatg aaatatccaa
    59041 caatttactg gttctctatg attatatgaa cacgagattg gtagaagcaa atataagtaa
    59101 ggataaggct attgttgaag aagtaatagg gtatgccgag gagcttaggg atacctgggc
    59161 acaggccatg aaactagcga aacaacaggc tgtagttaat cgctagtttg gagatacgag
    59221 atacaaataa gggacggcgc cctcgtcgtt cctaattttt ttggaggtta tttatggata
    59281 atacaaatag tgtaataatt gaactcctca gcctgacaga taagaaaaag cagttattcg
    59341 atagcatatt ggaaattact ttggcgcaaa aaaaagatat tgaagagaat gcagcaaata
    59401 aaatagaagg acttgtaagt aagaagcagt ctgtaattga cagcattgat gaaattgaca
    59461 agtctttttc cgaaaagttt aagctgctga aaaaacagtt aatggtcaat agcttggaag
    59521 aggttgattt tacaaaatat ccggaattga aaaatttaaa gcttaaagtt gaagaaataa
    59581 tgtcaatggc acaggaaata atgctcatag aaaggtccaa caaggagaag cttgtgtcca
    59641 taatgaatga aatgaaaaga gaaataaagc agataaaggc cgggaagaag tccataaagg
    59701 catatgaaag accaattaca aacaatgacg gaatatatgt agacaagaag aaatgacggt
    59761 attttctgat atttaatacc tatttgtttt caaaagtact tgttaaatca agaattacat
    59821 tatataatat taaatgtggt gaaatatcta ggtctgtggt tgaaaatcga tgccagtcgc
    59881 aggcaaaacg atccacgtaa gcagaagaag cttctgtgag catggtgcgg cttaggaata
    59941 agtccgtcca atagtttatt attgagaggt gctgtagtga ggggttgagt cctagtagcg
    60001 aaacatcctg acggcgagtg tggggtcaaa gaccaggtca gctagagtat tgccataaat
    60061 ttaaaggtat gagtgggcgg ttcaagaacg tttttcgcgg cgtaatgatc ccagaaagtt
    60121 ctttttaccc aacaccaacc tggagaagaa aagagaggga tttttacatg ttcgaagaca
    60181 aaacgttaat ctgcaaagac tgtggaaatg aattcacatt cacagcagga gaacaggaat
    60241 tctatgcaga gaagggtttc cagaacgaac cggccagatg caaagattgc agaatggcaa
    60301 gaaagaatgc cagcggaaga aatggacaga gaagagaagt cgtaatgtat gatacagtat
    60361 gcgcagattg cggcaaagaa acccaggttc cattcaaacc tacaaatgca agaccggtat
    60421 attgcaaaga gtgttttgac gcaaaaagac agtaattaaa accgcctatc aaggcggctt
    60481 tttattgtac aggaaagtat gctatcctgt gcataacttg tcccataccg tatacatcat
    60541 tttacgacag caaagtacct gcaaagggat tttaaaaccc actgaacgac aagataagtc
    60601 aaaataatat tttacacttt gttcgctcga catttttcag ccacaaatca attcacattt
    60661 tttcaacaat agaaagttaa tagattcaag ttatcaacag acttatcaac attatgcaca
    60721 gataacataa aaaagatgtg gataaccttg tgtataatgt gactaactaa gaaatatgag
    60781 gggtgggggc aggtttcctt taattattat ttttgttcgc tttacataac tttatagttt
    60841 caattatact tgcatttgct gctaaaatgc tgtatttttg cnnnnnnnnn nnnnnnnnnn
    60901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60961 nnnnnnnnnn nnnnnnnnnn ncggctgctg aatataaatg tttggaagtt atggaaatga
    61021 gaaggattat tttctcattt gtagaataaa atatgtataa acggtagcat aatagttgcg
    61081 aaaggagatg tgtttatgcg tactacagtc agcgggaaaa atattgttat caccaatgct
    61141 ctgaaggaaa gggtagaaaa gaagctttcc aagtttgaaa gatacttcgg ccctgatact
    61201 gaagtccagg ctactttgag cgtcgaaaag aacaggcaca tcattgaggt cacaattcct
    61261 tttaatgggg ttatcctgag aggagaagaa gctaccggag atatgtatac atctatagat
    61321 aacgttgtag aaaaattgga aaggcagatg aggcgccaaa agacaaagct ggaaaagaga
    61381 attaaagacg gcaacatcaa attcgagaac tgggccgccc ctccggcaga gttcgaggat
    61441 gaggaagtaa aaattgtaag gacaaaaaga tttgccatga agcctatgcc cgttgaagag
    61501 gcagttctgc agatggagct gctgggacac agcttctttg tgttttccaa tgcagatacg
    61561 gaagaagtca atgttgtata taagagaaag gacgggaact acggtttgat tgaaccggaa
    61621 ttctgataaa acagccgagc aaatcggctg tttttatttt aggagctaat gggcggagat
    61681 atggtattta ttctgtagca gttattatat gtatagtgca aattgcccat tcgatagtgc
    61741 aatttaacta atttatataa gagaagaagg attaaggaag tatttctaga aactattata
    61801 tcgcatgtct aatgttttcc gcggcatata taacttgagg aggagttccc tttgagcaat
    61861 aataaggcca ttctgtataa tgatatccaa tcacttatat gcaagcttcc cgatataata
    61921 aactgcagcg tcatatgtga caaggaaggt aaaattgaag agatccatat tttggctggg
    61981 gcaggcagga acatcaagca gatggtcagg gatatacaat cggcaataag tgcaaaattc
    62041 aatatatctg tggattataa aaaaatcagc attgcccaga tcaatgaggc tgagttcaag
    62101 gagacaagag tcaggataga aggtattcag gttaagaacg taaacaatgc gatagaagct
    62161 gaagtgacgc tttcctacga tgacaaagtt tatcaaggaa ggagcagcaa agtcaaatcc
    62221 aggtccaaca gagccaaagc agttgctgaa gcaacactgg cggccctgga ggcttgtatt
    62281 ggaataagcg gagtgctgta tttggagggg ctggaaaact ttaaggtttc agataaggag
    62341 gtatttgtgt ctttagtggg tttttctaag gacaatattg atgaatacta ttcaggaagc
    62401 agtataatca gtcttgacga gaatgaatct gcagttaaag ctgtactggc agcagtaaat
    62461 agaaaagtga acacagtagc ataaacaaag ggtcaaatat ggccggcaag ttatttaagc
    62521 ggagagtttg cctgccttga ctagcggtga ggcagagcct tgataaaagg agggcaaaca
    62581 atgaaaaaga tacttggtat tcttatggct attttcacat tggtattagc cggaggggca
    62641 caccagacct ggttttagga gtagcatagc atatgccggc cattttatga ggtgaataga
    62701 tgcctatcaa gcttaaagca tacgtagcag caatagtact attaggaaca atattttttg
    62761 tattcacatt aaatcaggcc cacgaggttg atgcagtggg tctggttatt tttaccctat
    62821 tatctgtaat tgccgaatca atggttattg ttacccaggg gcaaagggct ttgtctgtcg
    62881 ggtttataat cggccttgct gccatagtgg tctttggtgt gcctgaagct gcctggataa
    62941 ccagcatagg ggttatgctt agagttgtaa ggcatgaagg gaaaacatac catccattta
    63001 attttccaat agaaaaaaca ttgtttaacg gggcaaatgc tactatttcc gcgggattgg
    63061 cagggctttg ctatgaagcc ttgggggcgg ttcccggcac aatagattta aactcatcaa
    63121 tagctgcatt aattgcagtt ataacggcga tggtaatata tatactggtt aatgccgtaa
    63181 taatgtcaat tcttatgcat cttataaccg gagagaaatt tttttccaac ttcattaata
    63241 atatggcctg ggtggtgaag gattatttgg caatggcgcc ctttgctgtc ataatggcga
    63301 tggcttatgc aaattataag gtggcaggta ttttaatatt cttcattcca ttgttgtttg
    63361 ccaggtattc cttcaaaatg tatattgata tgaggagtat atatatagat acggtaaaat
    63421 ccctttcaca ggcgatagag gcgaaagacc cctatacaaa cggccattcc cggagagttg
    63481 gggaatacgc gtgcaagctg gctgaaaggc tggggctgcc tcctaaaaaa gttgagaacc
    63541 ttaagatagc ggctatactt catgatattg gcaaaatagg aattgatgaa agcatactta
    63601 acaagcccgg aaggctaacg acggaagaat ttgacaagat taagcagcat ccggagatcg
    63661 gggtcaaaat tattaaggat attgattttc ttaaggatgc ttccgaaatt atactcagcc
    63721 atcatgaaag atatgacggg acagggtatc ccgaaggcag gaagtataag gatataatac
    63781 ttgaagcaca aatactcagc attgctgatg tatttgatgc attgacttca gagcggccat
    63841 accgaaatgc aatgacagtt gaggaagccc ttgatattat tgagaaagga aagggcaacc
    63901 agttcgaaag caggcttgca gatactttta tcagaatgat aaaggatgat aaggagatga
    63961 aaaaggttgc tggttgaaag tgtaggcact tccctcgcag tcggaaagat gagaggagga
    64021 agcttctcaa atataaagga tgccagcctt gaaaaatggt atttttttgt tttgggattc
    64081 atggttgagt ttgcggcagt ttatgcagct tcaaggaata tagggttttt caaggacaat
    64141 attctaatcg tccatttcct gtcatatctt ctgcttttca ttggcattta tttcaatcgt
    64201 cggacattat ccttccgaat agtttttatc ggggtatttc ttaactttct tgtgataatg
    64261 gctaacggag ggcagatgcc ggtttcggcg gaggcaatgg cggatatagg tttgattgat
    64321 aatatgatag ccataagaga cggggaaata attacccaca cattaatgaa tggcaatact
    64381 gcactcaagt atctgggaga tattctggta ctggcgaaac cgtatcccag gccgaaaatt
    64441 ttcagcatag gggatgtatt catggctctg ggtatattca tgtatattca ggaaattatg
    64501 gtgaaaaagg ccgcaaagtg atgtgacaca ggggcatggg gggacacggg ggggacacgg
    64561 gggggacatg gggacgtttc tcctgtcccg ctttcagcgg gacaggagaa acgtccccat
    64621 gtcccccccg tgtcccgaaa cgtccccatg tctttttttg ttttttgcga attttacaga
    64681 ctatagcatg aaattatgat aaaatattaa agatatattg gaatccgtga taatcagggc
    64741 aggtgaatct aaatgccgca caacagctat aataatataa aaaaatatgc agcctttatt
    64801 tttatggttt ttacagtgtt ttcaggatca tctgaagtgt ttgattctga tatcaataca
    64861 tttgcggtga gcaggttcgt tgcacagaat gcaaggcttt cgctaagccg gacgcatatt
    64921 caaagctccg ttcagaataa tgagagcctg agaaagtatc cggatgcgga aaatattcaa
    64981 gcctgtgtat cgtttacaat actaatatgt attttaaata acataattat gaaaaatagc
    65041 atatggaaga atgcattatt ttgattatcc ttataaatat gtcagacagg gaactaaata
    65101 attatgagga gagataactg aatgttttta gataaagtct ttggcaatcc caatgataga
    65161 gagataaaaa aattaatgaa gacggtccag gagataaaca aattggagcc tgccatgcag
    65221 aagctcagca acgagcagct gcagagcaaa actgatgaat tcaagaaaag gcttgccgac
    65281 ggagagaccc tggatgattt gctggtggaa gcctatgcgg tggtaaggga agcttccgtg
    65341 agggttctgg gaatgcggca ttttgatgtg cagatgatgg gcggaatagt actgcatcag
    65401 ggcaggattg cagagatgaa aaccggtgaa ggaaagacgc tggttgcaac gctcccattg
    65461 tatctgaatg ctcttacagg cagaggtaca catcttgtta ccgttaacga ttacctggca
    65521 acataccaga gccaatggat ggggaagctg tacaactttc tcggaatgtc cgtggggctt
    65581 attgttcacg ggcttgaacc ggaagacagg cagaaggcct acagagctga tataacatac
    65641 gggacaaata atgaatacgg ctttgattac cttagggaca acatggttat atacagggac
    65701 caaatggttc agagagaact gaattacgcg attgtggatg aagtggacag tattctgata
    65761 gatgaagcca ggactcctct tataatttcc ggggtcggag aaaaatccac acagctttat
    65821 tatgtagctg atacttttgt cagagggctt aaaaatgaag ctgactttac aatagatgaa
    65881 aaggctcata ccgtagtcct gacagaggaa ggggtggcga aggccgaaag attcttcaac
    65941 cttgaaaatc ttgccgatgc caacaatatg gagatacagc accatataaa ccaggcactt
    66001 aaggcaagga acctcatgcg ccgtgatatt gactatgtaa ttaaggacgg gcagataata
    66061 attgtcgatg aattcaccgg caggcttatg ttcgggagaa gatacagcga cggcctgcat
    66121 caggccattg aggcaaagga aggcgtcaag gtggagagag agtcccagac tcttgcaacc
    66181 ataacctttc aaaactattt cagaatgtac ggcaagctgg ccggcatgac agggacggca
    66241 aagaccgaag aaaacgaatt cctggccatt tataacctgg atgtagttga aatacctaca
    66301 aacatgccaa tgataagaga agattatccc gatgcggtat ataagaacaa aagaggtaaa
    66361 ttcaatgcgg tagttgagga aattgtaagg ctccatgaaa aggggcagcc tgtcctggta
    66421 ggcaccatat ctgttgaaac ctctgaaatg ctcagcgata tgctgaaaaa gaggggagtg
    66481 ccccatgagg tattgaatgc caagcatcat gaaaaagaag ctcagatagt agctcaggca
    66541 ggacagctcg gcatggtcac catttcaacc aatatggcag gaagaggtac tgatattgta
    66601 ttgggagaag gagtaaagga actgggaggg ctccatatac tgggtaccga aaggcatgag
    66661 tcacgccgta tagacaatca gctgaggggg cgtgccggaa ggcaggggga ccagggttcc
    66721 agcaggttct ttatatcttt ggaagatgat ttgatgaggc tcttcggttc cgaaaaaata
    66781 atgaaaatag ttgaatccct gggaatggaa gaggatatga ccattgagca tccgctgctt
    66841 tcaaaatcca tagaaaacgc gcaaaaaagg gtggaaggca ggaactttga tataaggaag
    66901 catgtgctgc agtacgacaa tgtaatgaac aagcagagag aagtcatata cagccagagg
    66961 cgccaggtgc tgcttggcga ggacataagg gaaaacatca tggaaatgat tgccgaaata
    67021 ataggggatg cagttgccac ctatactata ggcagtgagt ttgcggaaga gtgggatatt
    67081 aaagggctga tggaatatct gcatacgatt ttccttccga agaatgcttt tgccagggaa
    67141 gaactgctga atttcaccag ggaagagctt actgaggcta ttaccggtaa ggctgtagat
    67201 atatatgaac agaaggagtc acaggttgat ccccaggtaa tgcgtgaact ggagagagtt
    67261 gcgctgctga gatccgttga tacaaagtgg atcgatcata tagacgcaat ggatcagctg
    67321 agagagggaa tatccctcag ggcctacggg cagaaggatc ccgttcaggc ataccagatt
    67381 gagggatatg atatgttcct ggagcttgta aaaaatataa aggaagatac ggtaagattc
    67441 atttatcatg taaatatttc aaaccttcct caaagggaaa aggtagctga acctgtagcc
    67501 acaaaccagg agggcggagt gagaaagcct gtagtaaagg aggacaaaat aggaagaaat
    67561 gacccctgcc cctgcggaag cggcaagaag tacaagaaat gctgcgggat gaataaataa
    67621 ggaggaataa tatatgttgg agcttgaaca gtataaatac ggcttatccg agatttggga
    67681 tgcaatagag gaaattgagg tgtccctttg acctgacatc gatacaggag aggatatacg
    67741 aactggagca ggagatgaac caggaaggtt tttggaatga tgccgaaaat gcaggaaagg
    67801 ttctgaaaac aataaaggct ttaaaggata aaacagaaaa aatagaaaac ctgaaaaaag
    67861 agtatgagga tattgaagta ttgatagagc tggggatgga agctcaggat gaatcggtga
    67921 tacctgaggt ggagaaatcc ttcaagactc ttcagaaaga tgtagagagc ttcagaattg
    67981 agacattgct caaggggcag tatgacagaa ataatgcgat attcacaata cactccgggg
    68041 caggaggact ggaagcccag gattgggctg aaatgctgct tcgcatgtac acaagatggg
    68101 ctgaaaggaa aggttatgct ataacaatcc ttgatatgct aagggattat gaagcaggca
    68161 taaagagtgc cgctttgctt atagaggggg aaaatgccta cggatatcta aaatcggaaa
    68221 agggagtgca caggcttgta aggatttcgc cttttgacac tgcgggaaaa aggcatacat
    68281 cctttgcatc ggtggacgtg atgcctgagc ttgatgatga agtaaaagta gagatagatg
    68341 aagaggattt gaagattgac acctaccgat ccagcggagc cggcgggcag cacgtcaata
    68401 aaacggattc agcggtacgg attacccata tacctacagg aataatagtc cagtgccaga
    68461 acgaacgatc ccagcacagc aataaggaaa tggctatgaa aatgctgttt gccaagctgc
    68521 tggaaattaa ggaacaggag cataaagata aaatagacga cataaagggc gagtaccgtg
    68581 aaattgcatg gggaagccag atacgctcct atgtattcca tccgtacaac ctggtaaagg
    68641 atcacaggtc gggggaagaa acgggaaata caggggcggt aatggacgga gaactggatg
    68701 atttcataaa tgcatatttg aagcagggga atggagtata attttactcc attcctatta
    68761 tttcaacctt tttgcagccc ttgcaggata gaagcagctc taccagttcc tcagggttta
    68821 tttccataat ttttgtatca atggatgccg tcagtgaagc tgagtccatc accggaatgt
    68881 tctgatttat ggttattata ttgcccttga cggaggctat tttgtttatt atatcggaaa
    68941 gcacccccgg gatatgcttg agaatcatga aaagtgtaac tgtacgcccc cgttcctggg
    69001 cagtatagtc aaatacgctg tccttgtact tataaaatgc acttcggctt actcctgcca
    69061 tacggcatgc ttcctggata gttgcggcgt ctcccgttct gagcaggagc tttgcttcaa
    69121 taaccttctc atatgcttta gggagtatgt cggaatcaac tatgaacatt tttttgttta
    69181 ccattacata ttgcctcgct ttctgtatgc aatcgggata catatgtacc tattttgtat
    69241 actatcatac actcggcgcc tattgcaata tccgtacaaa aatggttaat atataaagga
    69301 tgaaacaaca gagtgaagga gaataatata tggacattat tgcaaaactt gcaaaggaac
    69361 tgtcggtcaa gccctggcag gttgaaaaca cggtaaaact aatagatgac ggcaatacca
    69421 taccttttat tgcccgttac aggaaggaac ttacgggaga actttccgac acaacactga
    69481 gagagttggg tgagaggctt acatatctca gaaatcttga aaacagaaag gaagaagtct
    69541 taaggcttat cgaagggcag ggcaagctta ccggggagct gaaggcttcc attgaaaagg
    69601 ctgaggtatt gcaggaagtg gaggacatct atcgtccgta taagcaaaag aagaggacaa
    69661 gggcaaccat agccagggaa aaagggctgg agcctttggc ggacataata ttcagccagg
    69721 aacgccaatc aaaggctctg gaggaaattg cagaggcatt cgttaatgac gagcttggcg
    69781 tttcgacagt acaggatgcc ataaacggcg ctttggatat aattgcggag ataatatccg
    69841 acaatgcgga attcagaaag gaaataaggg agctgacctt cgggaacggc actattgttg
    69901 taaaaggcac caaggaagaa aaatcggaat atcagatgta ttatgatttc agcgagcctg
    69961 tcaaaaagat tgccccccat aggatactgg ccattaacag aggggaaaag gaagaatttt
    70021 tgaatgtcaa agcggaggca ccggcagaca ggatactgac ttatctgaac aacagcacaa
    70081 tcaaagagaa ttctccctat aaaaatatgc ttgaggaaac agtaagggat gcatatagca
    70141 ggcttattgc cccctccata gaaagggaga tcaggaataa tctcaccgag gaagcggagg
    70201 aacaggcgat aaaggtgttt gccgccaatc tcagaaacct tctgttgcag gcgcctatta
    70261 aaggaaaggt ggttatgggt tttgacccgg cttacagaac cggctgcaaa atcgcagtgg
    70321 tagatgatac cggaaagctt ctggaaactg ctgcggtata tcccactccg ccccagaatc
    70381 aggcggagga agccaaaatg attcttaaga agctgattaa aaagcataat gttgaagtaa
    70441 tatccctggg caacgggact gcatcaaggg agtccgaaaa gatatgcgcg gaaataatca
    70501 aggaaatgaa tatgcccctg cactatgtaa tagtcaatga agccggagca tcggtatatt
    70561 cggcatcgga gctggcatca caggaatacc ccgatataaa tgtatccctg agaggggcga
    70621 tttcaatagc aagaaggctg caggaccctc tggcagaatt ggtgaaaata gatcccaaat
    70681 ccatcggggt agggcaatac cagcatgacg ttgcacagaa gaagctgtcg gattccttga
    70741 atggagttgt ggaagactgc gtgaacagtg taggggtgga tctcaatacc gcatcaccgt
    70801 ctctcctgca gcatatatcc ggaataaact cagccattgc aaagaatata gtatcctaca
    70861 gggaagaaaa cggcaagttc aggagcagaa gagagctgct taaggtgaaa aagctcgggg
    70921 aaaaggcttt tgagcaggca gcaggctttt tgaggatacc tgaaagtgaa aatatattgg
    70981 ataacacttc ggtgcatccc gaatcctacg atgttgcagg aaagctcctt gagaaagcag
    71041 gcagggacat atccgctgcg gtggataagc tttcagcagt ggatgtaaat gagctggcgg
    71101 ctgagctggg agtgggggtc ccgacattaa gggatataat caatgagctt aaaaagcccg
    71161 gaagagaccc gagggatgaa atgccgaagc ctgtattgat gtcggatgtg ctggatatta
    71221 aggacttgaa gcccggtatg attctcaccg ggactgtaag gaatgttatt gacttcggtg
    71281 cctttgtgga tatcggagta caccaggacg gccttgtaca tatatcagag ctggctgaca
    71341 ggttcgtcag gaggcctctt gatgtagtgc aggtaggtga tatagtgaag gtcaaagttt
    71401 tggatgtgga ccttgacagg aacaggataa gcctcagcat gaagggcttg aattagccga
    71461 tgtatacatt tctatccaaa cagagtataa tatcaatagc aatcgcgacg gaggtatatc
    71521 aatgctgaaa tttcatactg agagcgctga agaaactgcc aaaatcggcg aagggctggg
    71581 aaggcttctg agccccgggg atattgtgtg cctctcggga gaccttggag cagggaagac
    71641 tgcttttacc caaggtatag caagaggact ggaagtcaag gattatgtta ccagccccac
    71701 ctataccatc attaatgaat atgaaggcag agtgccttta taccactttg acgtatacag
    71761 gcttaatgat gttgaagaaa tgtacgaatt aggctatgag gaatatttct tcggtgacgg
    71821 ggttgttgtg ctggaatggg cggatatggt aagtgacata atcccggaag ataggctttg
    71881 gattacaata ctgaatacaa agggagacaa ttcccgtgaa ataataatgg agccggccgg
    71941 agaagcatat gaagctattg tgaaaggaat ggaatccaat gaaaatactg gctgttgata
    72001 cgtccacttc cgccgcaaca gcggcaataa tggaagacga cagactgata tccgaataca
    72061 tattaaacat cagcagagcc cactcccgga agataatggg cataatagag gagttgttca
    72121 acaaatccgg aattaagcct gctgaaatag atatatacgc ttgctctgcc gggcctggtt
    72181 cttttacggg gctgcgcata ggagcatcaa ttataaaggc tatggctcaa accttcgata
    72241 agcctgttgt cggagttccg actcttgatg cactggcctg caacctttac aattgtacag
    72301 gcctggtttg cccgatgctg gatgcccaaa gggggatggt atacagcagc ttgtactcgt
    72361 ataattccgg caggctgaca aaactggagg atcttggggt aataccgata gaggatttga
    72421 tagagctggt gaagagtaag gctgtaccca caaccttcct tggggatggt gccgtaattt
    72481 ttgaagaaaa cttgaaaaaa ggcctggaag ggtataatgc ggcacccgca ggcagcttgc
    72541 tgccaagagc ttcctcctgc gcaaggatgg ctttggaaat gtataataag ggttcctttg
    72601 atacctacaa ttctttcagg cttacctata tcaggaaatc ccaggcagaa gcggaatatg
    72661 agaaaaagaa caaagcagaa tttcaaagca tgagcctgga tgatattgac caggttgttg
    72721 aaatagagaa cctgagcttt gcttctccgt ggtccaggga atcctttgaa tcagagcttt
    72781 tgaaaaacaa acttgccagg tatatagtgg ccaaggtgaa tggcaaggtt gcagcctatg
    72841 gaggaatgtg gattatactg gatgaggcac atataacaaa tattgcagta catcccgaat
    72901 tcagggagcg taaaataggg gagaaactgg taatggagat gctccggacg gcaaaggaaa
    72961 acaaggcgga gcatattaca ttggaggtaa gagcttcaaa tgatgcnnnn nnnnnnnnnn
    73021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    73081 nnnnnnnnnn nnnnnnnnnn nnnnnnttca ggatgatggg aaacagggaa gaagccgagg
    73141 atgtgtcgca ggaggcattt atcaggatgt ataattcctt gtccaaatac aatcctgaat
    73201 acaaattcat aacctgggcc ttaaaggtta caacaaatct ttgtttagac agcttaagga
    73261 aaagaaaagc agaaacggtt cccatagatg atgtctttga aatcaaggat gggaaagata
    73321 ccccggaaga agaatacata aggaaagaga atcaaaagct ggtgcaggat gccataatga
    73381 aactgccgga taagtatagg gaatttctga ttctattcca tcacaggaac ctatcctatc
    73441 aggaaataat ggatattacg ggtgaatccc ttaccatagt taaaaacagg ctttacagag
    73501 cccggcaaat gcttaaggaa gaattaatca gcagggaaaa ggagcgtgaa aagtatgcag
    73561 tgccaggaaa taaataattt ggtcatgaag tatttcgacg gcaatatcag tgaactggag
    73621 cgggaaatga taataaagca taacgagaaa tgcaaccgct gtgctgaaga atttgggata
    73681 ctcaggaatg ccatagatac tttggaggag cttcctgaaa tagaagtgcc tgccgggttt
    73741 gagtctaggg taatggaagg gattttgagc aggagagcat actcaacgaa tcctaagata
    73801 gttgctttct ggctactctc ggtacttgga ctcatggttt tcggatggaa tatggcggca
    73861 tatgtaataa tgcccttgat aagagaaagc ggaatcatta ttgccgccaa taatgttctt
    73921 atatacggtt tcaacgtggt ttccggcatt ctgagagagg tagttgttac ggtttcaaag
    73981 ctgctgggga agattcttgt tatgagaaac gtcctgctca gggactatat aacgagtgtt
    74041 actttgatag tactggcatt tatggctatc aatgtgtttt tggcatacag gcgtaaaccg
    74101 caagagaatt aggaggtatt tatatgaaag tgaaaaagag ggcagcattg ttggcgggtt
    74161 tgatgctgat ggcggtaatg ctttgcgcct ttgaaatcaa tgtggacaat aaggataaag
    74221 tggcgatatt taaagatata gagattcctg agggagttac tgttaacgga gatgtagttg
    74281 cattgttcgg ggatgtggct gttagcgggg atttaacggg agatgcggtg acaatattcg
    74341 gagatatgtc agtctcgggg gctatagaag gggatgttgt tgcagtcttc ggggagatat
    74401 ccgtaaagga cggcggcata attagcggag atgcggtcgg aatactggga ggagtggata
    74461 aatc
//
LOCUS       DAOOAB010000008        69735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_462_length_69735_cov_14.832649, whole genome shotgun sequence.
ACCESSION   DAOOAB010000008 DAOOAB010000000
VERSION     DAOOAB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 69735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69735
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_69735_cov_14.832649"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKU88_03135"
     CDS             <1..315
                     /locus_tag="PKU88_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPX68309.1"
                     /translation="KLAIAAFLIPYIFVINPQLLLINTTGLEVIQITITSLIGMFGIG
                     IAMEGFIFGKVNPLIRLIFMAGGLCLIDPGVYTDMAGIIAIGLGLAYQWFKYKRTTAK
                     SI"
     gene            complement(347..778)
                     /locus_tag="PKU88_03140"
     CDS             complement(347..778)
                     /locus_tag="PKU88_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68310.1"
                     /translation="MDKYNILVLMEKNRATGFFIQTIDSYKIDSGLELIENAYLSEEE
                     GEYFIFLVLTTADVEDYQYNGIYDLYNEEVFSKFNIDLLDGSGEYNPRWIVKLKYNEK
                     RSEMESIINELLEIHKAELQRVLPLVEADKTKYLEEFEKEE"
     gene            895..1599
                     /gene="sfsA"
                     /locus_tag="PKU88_03145"
     CDS             895..1599
                     /gene="sfsA"
                     /locus_tag="PKU88_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HPX68311.1"
                     /translation="MKIINKTINGRFVGRLNRFEGIAEINGGEVPVHIPNTGRCRELL
                     FKGAGIILEIRESKTRKTPYELIMVYKGDRLISIDSQAPNKIVEEAVRGGLIEEIGSY
                     DYVKREAFYGQSRFDLLLKKTEGSGKADSCYMEVKGVTLEINGTALFPDAPTERGARH
                     MKELAAARKEGYRAVVIFLVQMEGIKCFTPNKLMDSGFAEALADAHSEGVEVLSYNCR
                     VSEKEIVINERVEVVL"
     regulatory      1750..1918
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            2239..3573
                     /gene="mgtE"
                     /locus_tag="PKU88_03150"
     CDS             2239..3573
                     /gene="mgtE"
                     /locus_tag="PKU88_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPX68312.1"
                     /translation="MENIIKDYIEVKNYSAIKKALEDMLPADIAELLEELDTKNSLLV
                     FRMLHKDVAVEVFAHLSVKQQTSFTLMIKEDELKEILEELYFDDKIDYLEEMPSSVVK
                     KILKYSPETERKLINQFLNYPENSAGSLMTIELVDLKKELTIDEALLRIRRTALDKET
                     IYTCYVTDSNRKLEGTVSLKDLVIAPGNKKVEDIMETDPIFVRTIDDQEEVANLFKKY
                     DLLSVPVVDQENRLIGIITIDDIVDVIEEEASEDIQIMHGLAPSEEEYLKTDIITLAK
                     QRIFWLLFLMLSATITGNIIESFQGSLEKAVGLAVFIPMLMGTGGNAGTQSSTLVIRG
                     IALGEVVLSDISKVIWRELRISLMVGISLAVVNFVRVYYLGGNSFTVSVVVSVTLILT
                     IMIAKVIGGVLPLIAKAINIDPAIMASPLISTIVDALTLLSYFKIAEAIMHI"
     gene            complement(3693..5075)
                     /locus_tag="PKU88_03155"
     CDS             complement(3693..5075)
                     /locus_tag="PKU88_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPX68313.1"
                     /translation="MKDRIKIIKYIYLSLFALICLYLLYFSLFESRLIASNSYNRRLR
                     ESEGNIIRGSILDKNGEILAKSEKYKAGVKRTYVYPRYFSHVIGYSSAKYGSSGLEAL
                     YNKELMSASDTMEDLRSKINDYYIKGNNINLTLDRSLQVYASELLRGKKGAVVVLKPD
                     TGEILALVSKPDFDPSGIQNNWESLINDKKSPLLNRASEGLYPPGSIFKLAVASSILK
                     NNLQDIEVECTGEVNADGYIIKDSDGKAHGSITLSEALKHSCNSYFIKQGLKLGNSNL
                     YNEASRFRLNKKIQTDIRVSESVFPSKSDNRSIAQQSIGQGEVLMTPLHAAMMAAAIA
                     NDGVMMQPYVVSSISGSENKIIKEFRPKQIGRIMDKNTAEAIKDMMVEVVESGTGTRA
                     KVSKVAVAGKTGTAEVSGNQKSHAWFVGFAPADSPKAAIAVLIENGGGGGSAAAPIAS
                     KILKKALSLR"
     gene            complement(5087..6304)
                     /locus_tag="PKU88_03160"
     CDS             complement(5087..6304)
                     /locus_tag="PKU88_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPX68314.1"
                     /translation="MLNLVLIAMNFALISYYSEPLDYMALYICGGLAAAYMISRMILA
                     KLLNDGNCLMFDVVCNMLQTGLAMLYRLSPEYGTKQAYIAAAGILGFFASFIFMKRFE
                     VKYKHHLLLGGLILALLLTTQIWGIEINGSKNWINLYFTNIQPSEFIKLIFVFFTACL
                     LSREPSRKRFAGIAASMLTVTAFFVLQRDIGSAFLFFTVYLVMLFSRDVRGLYTAASS
                     GAALGGAFISYFAFSYIRSRVLAWINPWKYISGKSYQITQSLFAVATGGFIGTGLYLG
                     DPKFIPAVHTDFIFSALCEETGILNGISLLAMYALLSIIGMDISSKCSNPIYSSAALG
                     LTSLTAIQTLVIVCGVLNIIPITGVTLPFVSYGGSSLLSQFMNVGILYYISTDKASQT
                     SEAESLVPRTASR"
     gene            6562..7200
                     /gene="ppaX"
                     /locus_tag="PKU88_03165"
     CDS             6562..7200
                     /gene="ppaX"
                     /locus_tag="PKU88_03165"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase PpaX"
                     /protein_id="HPX68315.1"
                     /translation="MGYKYVLFDLDGTLINTNRLILDSFKYTYKKCLGLEVCEEDILK
                     CFGEPLKVTLRRYSEEKADELFNTYIEYNESRHNDTVTIFDGIQELLSELVNQGRTLA
                     LVTAKRRRVAIMGLELFDIKKYFDVIVALEDTELHKPNPAPVIKALELLGAKPSETLM
                     VGDSVFDIHCAHGASVAAALVKWSAARDFQDDASSADYVVHDTKELLDIIIG"
     gene            complement(7494..8294)
                     /locus_tag="PKU88_03170"
     CDS             complement(7494..8294)
                     /locus_tag="PKU88_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68316.1"
                     /translation="MYEEYSNEHREYIRKERITKNAVTISRIIILSAIFALWEIAANM
                     KWIDPFIMSQPSRIVNTVINLSKDGSLFLHTGVTIYETIIGFVSGTVLGTLAAIVLWW
                     SNFTARVLDPYLVVLNALPKTALGPIILVWIGGTTGSIIVMALLLSIIVTILNVYQSF
                     RSCDEDKIKLLKTFGATKVQILRKVVFPSSIPEIISTLKINVGLSLVGVIVGEFLVSK
                     AGLGYLIIYGGQVFKMDLVMTSVIILAVAAAFMYLSVSWIEKKFVKMR"
     gene            complement(8287..9057)
                     /locus_tag="PKU88_03175"
     CDS             complement(8287..9057)
                     /locus_tag="PKU88_03175"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68317.1"
                     /translation="MDKVVELKNVTMNYHTLDGETQAIKNLSLDIRRGEFVSIVGPSG
                     CGKSTLLSLISGLIKSSTGEILINNEPVKGLASNVGYMPQRDHLFEWLTILRNTTIGL
                     EIQKKLNKDTMRNAERLLEEYGLGEFKYYYPNQLSGGMRQRAALIRTLAVNPEILLLD
                     EPFSSLDYQTRLAISEEIWLIIKKEKKTAILVTHDIAEAVSMSDRIIVLTRRPGEVKS
                     EHVIQLTTCENKTPIICREAPEFRIYFNKIWKELDVHV"
     gene            9252..10283
                     /gene="mnmH"
                     /locus_tag="PKU88_03180"
     CDS             9252..10283
                     /gene="mnmH"
                     /locus_tag="PKU88_03180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPX68318.1"
                     /translation="MYNTIDYEQLEGDYVLVDVRSPKEYSEFTIPGAVNLPIFNDEER
                     ELIGTVYNCGSIDEAKKMGVDFASKKLPLLYEEIKRLREGHDKVILFCERGGYRSSSL
                     CALLNSIGIGAVKLRGGYKGYRAVVNSMLPKLNREVNYIVIHGYTGTGKTKLLKMLES
                     RGYDILDFEMHANHRGSLLGDVGLGKRISQKQFESLVYDSLKKRKSDMIFVEGESSRI
                     GNIVIPHYIMQSMREGKHILAVGSIEMRVRRIVEEYTKEKNSDKDIINSLEKLGRNIS
                     ARRVEEYARDIRLRNYHQVAEDLMIRYYDPMYENEMKEFEYELTVNTDNIQKACLDME
                     KWLKKAKPE"
     gene            complement(10312..11331)
                     /locus_tag="PKU88_03185"
     CDS             complement(10312..11331)
                     /locus_tag="PKU88_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX68319.1"
                     /translation="MRKSAKLVSLVLVVLIVLSSAAMLSAGCSRKELTTVRLNEVVRS
                     IFYAPQYVAINKGFFEEQGLEIELTTGQGADKTMTALLSGQCDIGFAGPEASIYVYEQ
                     GKEDYAVIFAQLTQRDGSFLVARNDEPEFKWENTRGKVIIGGRPGGVPEMTLEYVLKK
                     YGIIPGKDVEIITNLQFTATAGAFISGVGDYVALFEPTASMLEKEGAGKIVSSLGEES
                     GLIAYTAYFTSESVIDKNPEMIQKFTNAFYKGQLWVHSHTAEEIAEAIKSFFPDADDD
                     ILVTVVKRYKEIDAWCTDPITTPESLDLLQEVMQTAGELDKKVEYDKIVTQEFARKAI
                     ETVKP"
     gene            11575..12108
                     /locus_tag="PKU88_03190"
     CDS             11575..12108
                     /locus_tag="PKU88_03190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPX68320.1"
                     /translation="MNIPNALTIFRLILIPGFVYYYFSSMPNGDRIAIVLFAAAGVTD
                     ILDGFIARRCNLITRLGTVLDPLADKLMLLTVLISATMKNQISFWIIIVVAIKETLLI
                     LGAITLFNDHDIVVPANRFGKLSTIAFYIAIIAVAFDIPYSRVMLDGFVIITILALVV
                     YANCYINIKRRQRIYKT"
     misc_binding    12267..12478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12539..15196
                     /locus_tag="PKU88_03195"
     CDS             12539..15196
                     /locus_tag="PKU88_03195"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPX68321.1"
                     /translation="MDESKNIAKTYDPKQVEERLYEIWMEKGYFTPVIDKNKEPYTIV
                     IPPPNITGQLHMGHAMDNTIQDILIRWRRMQGYCTLWLPGTDHASIATEAKIVETLAK
                     EGKTKYDLGREKYLEMAWDWKHEYGGRILNQLKKLGSSCDWTRERFTLDEGCSDAVLE
                     VFVRLYEKGLIYRGERIINWCPKCKTSISDIETIYDTAKGHFWHVKYPVKDSDEYIEI
                     ATTRPETILGDTAVAVNPNDDRYRHLVGKTLILPLMNREIPVIEDEYVDMEFGTGAVK
                     ITPAHDPNDFEVGTRHNLPMPRILNDDGTINSLGGKYKGLDRYDARKQIVKDLEEQEL
                     LVKTKEHEHNVGHCQRCSTTIEPLLSKQWFVKMKPLAEPAIEVVRGGRVKFVPERFTK
                     IYYNWMENIQDWCISRQLWWGHRIPAYYCEDCGEVIVSKTAPGSCKKCGGKLKQDEDT
                     LDTWFSSALWPFSTLGWPEKTEDLEYFYPTSVLVTGYDIIFFWVARMIFSAMENMQEE
                     PFRHVLIHGIIRDSEGRKMSKSLGNGIDPLDVIDKYGADALRFNLISGNSPGNDMRFF
                     WEKVEAYSNFANKIWNASRFVLMNLDYDRVKNAVPSEYSSSLTAADKWIISRFNTVVK
                     EVTENMEKFELGIAAQKIYDFMWSEFCDWYIELVKPRLYGDDEETKLVAQATLCDILK
                     GTMQLLHPFMPFITEEIYRHLPSEYESIVISRWPEYNGQAYQPEEERRMEKVMEAIRG
                     IRNVRAEMNVVPSRKAKAIVVTADKDVKEAIEEGRLYFERLASASEVVIQDSKEGIPE
                     GAVSVLINGAEIYLPLEDLIDFEKELERLNKEKDNLEKELQRVKGKLSNQGFIGKAPR
                     SVIEEERTKEKKYQEMLNKIYERINSLKK"
     gene            15213..16508
                     /locus_tag="PKU88_03200"
     CDS             15213..16508
                     /locus_tag="PKU88_03200"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPX68322.1"
                     /translation="MNYNEVVDLIDTTVKFGCKPGLERTEKLLELLGNPHKKLRVIHV
                     AGTNGKGSTAAMLASILSSAGYRTGMYISPHLFRNTERMTIDGIEITEEDFAEYATQV
                     LSMVRLMGEKGLEEPTQFEMYTAMAFLYFERKKVDFAVVEVGLGGRFDATNAAEPLLS
                     VITSIGYDHKDVLGDTIEKIAYEKAGIIKEGSMAVMYPQPYPEASAVIETVCREKGAI
                     LAEADIDSLILKDYGLSGQTVDFKYRDYDIRGMKLPLIGDHQLYNAAVALTAIAELDK
                     MGHRIEEAGIRKGIGSVKWPCRLSIVSIEPLILVDGAHNEDGINSLRNALLKYFGNRK
                     IIFVIGMLRDKDYAYAIRKLMPMAYHVVAAEPFSERALSASAMAEAVKPYCSSVISEP
                     DIKKAIEKAKKLYEKDCMICICGSLYLAGRAYEYLTEKN"
     gene            complement(16613..17701)
                     /gene="ychF"
                     /locus_tag="PKU88_03205"
     CDS             complement(16613..17701)
                     /gene="ychF"
                     /locus_tag="PKU88_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPX68323.1"
                     /translation="MKIGLVGLPLVGKTTFFNLLTNAHAEISNFASGKVASNIGSAKI
                     PDRRIDFLSKLYKPKKTTFAMIDFTDVPGLVSGASTGAGVGNQFLEDIRKVDALVHIV
                     RAFENTDVLHIEGSIDPMRDIETVNLELLFADLGIIDNRIDRIKSGKKVTKENLEELK
                     VLEKCKEGLENGLLIHQINLSEEERDLLKTFSFLSEKPMILVINIDEKSLKSDSYPQK
                     EDIRKYAAERNVPVIEVCAKAEMEINELDEEDRLMFMEELGIKSSGIDILANTTYDYL
                     GLISFLTAGEDEVKAWTIRKGTDAKKAAGKIHSDIERGFIRAEVVKFKDIEALGSMAK
                     VKEKGLYRLEGKEYIVQDGDIINFRFNV"
     gene            complement(17723..18247)
                     /locus_tag="PKU88_03210"
     CDS             complement(17723..18247)
                     /locus_tag="PKU88_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HPX68324.1"
                     /translation="MISDSGALADKKLMILYLLSKVGIPLTKSQITNAILENNLIDFF
                     TFQQCISELEESGMIKHTQYQKRQCCKATDSGIRAVGIFEQRISKNTAQIINNYISKN
                     KESLRKESQVIAEYKKMDDKEYIVSLKVIENELVLIDLKLNVVSAKQAKQICEKWKNS
                     SENIYSQLIGSLIN"
     gene            complement(18382..18606)
                     /locus_tag="PKU88_03215"
     CDS             complement(18382..18606)
                     /locus_tag="PKU88_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPX68325.1"
                     /translation="MAAGSGKYNKGLKVPNAHKALDNMKYEIASELNLPVHQGSEDYW
                     GNLSAKDCGRVGGNMVKKLITMAEQQLEGN"
     gene            complement(18817..19005)
                     /locus_tag="PKU88_03220"
     CDS             complement(18817..19005)
                     /locus_tag="PKU88_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68326.1"
                     /translation="MSNRRSKFTNIAGNMVEFSRELILPQDRHFHREAKSQKVQQQGV
                     HRNGTNKGRKIPIKRKMT"
     gene            19190..20284
                     /locus_tag="PKU88_03225"
     CDS             19190..20284
                     /locus_tag="PKU88_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPX68327.1"
                     /translation="MKQLKHIIAFFFIIIIVLGFMACTPQIAEEHPRNPEENLQEPEE
                     PAEVEEPDEADEPKADPVDLKAIKANENGQVMILMYHGIGEKEAVWMRTAENFRKDLE
                     VLYNKGYRLISLKDLIENNIKVEAGYTPFVLTFDDGAENNFNIIEENGEKKIDPNCAV
                     GILEEFKKEHPDFGSGGSFFIYYPIPFRQKELIKEKYEFLIANGYDIGNHSYNHENLG
                     KLSIEGIQKSLAKNVKSTQEYLPDYDVYALALPNGIGPKGDDYKYAATGEYEGVKYNN
                     RAVLKVGSNPAPSPVNVKFDPIRLPRVRASEMETEGVGMYDWLQYFEKKPEKRYISDG
                     NPDTVAIPESEAEYVDKSRLNGKELVIYKE"
     gene            complement(20591..20848)
                     /locus_tag="PKU88_03230"
     CDS             complement(20591..20848)
                     /locus_tag="PKU88_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68328.1"
                     /translation="MKKKRKSNCKGRGFEQISFRDIDLRIGDKLYKDGRLYAEVMGES
                     EELYFLQKSGSSCEMPNPYFKETIIENILFGRLFLEEMRFQ"
     gene            21099..22355
                     /locus_tag="PKU88_03235"
     CDS             21099..22355
                     /locus_tag="PKU88_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68329.1"
                     /translation="MRLLSALYHEGIEEYIESIPGIEVLDVVGNKRSLLDSLSKDKYD
                     LVLISKSLPGNEEADLLIEMLASEEFKGLRTAYLYGEYDGYCDDFIKFLINHGIYDFH
                     VGDEITSKDIERIIFKPASKERALYYFRSHYDNEMNIHYPEQKNKRNKRFFEGRFLFN
                     SGFGSLFSKNHKKNPKEKLIISIISNQATGKSHTAWNLSHCFSKLGYATSLFNIDRGY
                     SANLFFDIDEVYYNLLDFTLQNDIHKDILENCYNRKNLCVVTGKLGDEKEISSEDFLK
                     LLYCIRTKSDITIIDTRTGISELTRLSMQNSIYDMLVFDCDIMHFHMNMKMLEELKDD
                     FVPEKTIAVINNTNIRSSSHKFIYNELTSSGIPFKDMAFVSSCGFISYEVMHTGYTPY
                     QAAADRNKSFLGDMDSLLDKLGISNE"
     gene            22370..23059
                     /locus_tag="PKU88_03240"
     CDS             22370..23059
                     /locus_tag="PKU88_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HPX68330.1"
                     /translation="MAYSKKNKVFAVKRTALISGCVVCAVLILSAVYMLYGNVFSGEA
                     AGTVRNSSENEAKTVVIARAKENLGQGELLAASMAELIEIPVELAPEGAIKSLSKLNN
                     MRLKRGIAKNEFFNDMDLMPQNAFYEKGERLIEHNFAEGAVPASVTAGSIIDIKLFVK
                     GGEDGIVVSKAAVISRSGNLLSFYLDGMEQEFIKEAAAEGALFAVQYIDASQQASEVT
                     YTPSYSKDKQN"
     gene            23075..23926
                     /locus_tag="PKU88_03245"
     CDS             23075..23926
                     /locus_tag="PKU88_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68331.1"
                     /translation="MRGAKGKLICTWSPMLHGEGCSTLACGIGIGLQYLSGKKVLIVN
                     KSNSASHMERYVEKDIEIKYSMDNLKIFNKEIKTEHIFTYATRINKDLYMIAGSRLNR
                     DIIGENSEFDKIFLEKCLAGFDLVITDLDTGVRDDNRTYLDQADKILAVVTPNEIIID
                     KLYDEYRVRDALGYFTDDKAVVIINKLCEGWDTAGVIGRYKNRYSLSDIFGLNYDGDV
                     LNACCTDKNFYSFLMKEAKREKNIFIKQLSEICRFLIEGLCMGNNAADRIKHGNIFKK
                     FLKSSIY"
     gene            23943..25679
                     /locus_tag="PKU88_03250"
     CDS             23943..25679
                     /locus_tag="PKU88_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPX68332.1"
                     /translation="MDLGKYLIIDSLCLHMEKYINSFINSEPSDEQEKSIVSLLRDYK
                     EKSTSGLPEAREVIKSHIKTGILTGFDIYVSNEMGRLEDAIMLKGVCVDEANGLADLI
                     LPFNEPQKLTSREKFEIILHKNRNSSGNGRDGAFKAILARYSCCCKAREAAGYRSLRY
                     EYDEADIDYIYNNEDYVLSFSDKIEIITQRLYEEIFGLKHIDMLAYSDINEVGFSNNG
                     RYVYCWSGKKIWLSFLQISEKDARVIQDRAISFEKHCPQLDVSHPEILCHRGDGARIT
                     VTQKPYFSARNLCIRIFNQSCADFNDLITADKLRTLIIALVKTGESICLQGGLGTGKT
                     TTMNVMYELLDDLLHIGTIEDYFEQHIMEKYPYKRIVEGQAVNGKDLLDVVKTMLRMS
                     VDVANIGEVRDGNALFAFIQLVQSVSVAAWFTTHITNPETTVPRLKNMLIGTGRYFSE
                     QSAIMDIVHYINMIFQHEIIDGRITITKAVEIVPLVAVSSDGEYDIETPREQLEKLFY
                     IQQLRSNTYNMFRLNTIMQSVDGKAKFVNYPSRRMVKKAKGDRQSWEYLKNLIRLIEK
                     DTGMAIPEYARG"
     gene            25688..26512
                     /locus_tag="PKU88_03255"
     CDS             25688..26512
                     /locus_tag="PKU88_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68333.1"
                     /translation="MRNYYSKGIFHALFAGLNNRLTRINQKSPAQNARTSLALLTVLT
                     ILVIIYFLLACSTFKGLWYHRVLCGFSAFIFMYLCKESYFEIYEKRIKRDLPNTLKKL
                     THYYNHYRGNIIPALEDTVSRCPRSNRIYMIEIREALMKPDYQVQIEELEGKMPMIWL
                     KMLCRLILSAKENGGMSSEGDTVANNLKRMTNIITFLNIEQGYNDAELLGMQIFVFFT
                     PFLVIPLTKWYNISLLSGLNIGDIYGSIQAQGLTAIMMFISGVGTIFIHWMRKLQN"
     gene            26521..27687
                     /locus_tag="PKU88_03260"
     CDS             26521..27687
                     /locus_tag="PKU88_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68334.1"
                     /translation="MSNIQYFILIQAIAIALLIAAASIFVHQYFRYRKIKRLYRADYA
                     RKSCLKSISRFLIFMPGYKSRRYKNTEGLIINSGLRVSVEEFYLAKTLLFVLGFVFFI
                     SVQTTNTFAFYDHVISDLNMGNTLIDEAAPNDYTLQLEKEVFIHIDSSLPKGKAALKE
                     LKDENNSQVYIGYIEAQISSKWAGLEEYAGEMAERMYKKLIKIRRIETDYLIYLTSFI
                     LAIILYFIPNIIGHLKLILIEDKRDWEILNYIYVFSMFGRMPPFNIKNVLSNILVISD
                     IYKPIINEALNNLNSGKGEEAFDNLLQKVENQELYELLESMRLSMSTGLLNIVDNIDE
                     MAVNQLKWLEIKSLKRRKSKQVIAMVPVVLVMLAAAVYFSYSLSTLSNPMNYLK"
     gene            27707..28063
                     /locus_tag="PKU88_03265"
     CDS             27707..28063
                     /locus_tag="PKU88_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68335.1"
                     /translation="MEKFWAVILIFVVVAAAGLMYVNQVYIPQKETQMAMADMANQTL
                     SAVKVEIGDDSVPQEVSGSEIKDIIEDYSDDDIKVYINGIIYDGTNVIEHSSQYYMRV
                     IKNDGEITEIRFTIIV"
     gene            28214..28660
                     /locus_tag="PKU88_03270"
     CDS             28214..28660
                     /locus_tag="PKU88_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68336.1"
                     /translation="MEKFWASILVIPIFTWYSFHAVLDRDFRIKQQAVENIVCQYTQI
                     AAKKGALYNSVYNEMKERLSRFGTFDISLTAERFTGSSDTPELIKGSYIIGRDLRSEG
                     FDIINIYAESINRHPLGRLYELTPFGTKSGAEADIRYFGKASVYIQ"
     gene            28677..29465
                     /locus_tag="PKU88_03275"
     CDS             28677..29465
                     /locus_tag="PKU88_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68337.1"
                     /translation="MKSFFWSLIFIFCTWSMFFIGKGVDNIDIMAERYKRALDAGANA
                     ASGYRAYYSEKMLYNHGTGFGIGLEDSNNLPVDREEAVKWFYRLFFRNLGIEDTNKQN
                     EYKRYMPMKAIILFDRLMIADADDNWHTYDPAGEKEYMIQYRGKDYKFTLSDQVYDIE
                     NSSWIMADDIGLEAKERKELVTNYIINELNSFLDNRGNKESGSYYKIVFSLDDASDSK
                     LSGINGINFFILCEGIPIPSLNPFKQEKFFAYSVGGSEIKKKQL"
     gene            29717..31621
                     /locus_tag="PKU88_03280"
     CDS             29717..31621
                     /locus_tag="PKU88_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPX68338.1"
                     /translation="MSVLPYITAVDNRVEDVNKPFLDLTGYTESSIRGRNVEEIWQQL
                     LRISMDLDSVKCLKECSCYMFDILRRAKEVTISYTADFELKQCIYTFKRKVNSMLEDK
                     FPFLEHANSANDTGVALYSVPEFILLKGNESHNRFLSRLLGGAEDIIGKRIEEILPDW
                     KGSDAENIFSNVVKTGKPIFYDEHMREHPSTGIVCTRYTLTPIFEGEELRYLVLIHND
                     VTKNLTIEREKDYFEKQRDYLYRLFNAMNLPIISFSYPEYRIKEFNNKALVELSELTG
                     MDNLTIDPSISCKGLAEMIPLFDISSAKILIEQMKQTKSPVCHKKMELCKKGRKVYYD
                     VTFHPFLNIMEEISEILIVAADVTDEVEKAKHIENILKLKDEFLYFITHEFKTPLSVI
                     NAAVQTLEHCYSMQIPDKARFLIGKVKQNSYRQLRLVNNLLEIARINSGYIKLRQRNV
                     DIVFLTRAITESVAIYAQQKGIELVFVTKFPQRVIGLDDEKLERVLLNLLSNAIKFTP
                     AGGKVVVELSSKLHKHKRVICIKVKDQGVGIPKEKQSVIFDRFGQVDNLLTVQAEGSG
                     IGLYLVKLMVDSIGGEITLESEWGKGSVFTLMIPSKKSKENAKEQKFKLRSDNRLIQT
                     TAIEFSDIYL"
     gene            complement(31626..32096)
                     /locus_tag="PKU88_03285"
     CDS             complement(31626..32096)
                     /locus_tag="PKU88_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPX68339.1"
                     /translation="MSKSIISKKIYEMLVNHLVRIEEEKLHVLDKYYPVWTNERNDFQ
                     ELLDSYIKGVENIIYNEVLVDDNASDTCPFAIIGSQVILKDCYTNKIEEMQIVSPFMG
                     EADLNIDSASYLSPMGRAFLLKKVKDDVKVETPMGIFQYIVENITLPSDAIDSN"
     gene            32669..33304
                     /gene="cysE"
                     /locus_tag="PKU88_03290"
     CDS             32669..33304
                     /gene="cysE"
                     /locus_tag="PKU88_03290"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPX68340.1"
                     /translation="MFGRLKEDIRTIFERDPAAKSYLEVILCYPGLHAVICHRIAHGL
                     YRRRRYVAARLISQISRFFTGIEIHPGAAIGRRFFIDHGSGIVIGETTEIGDDVTLYQ
                     GVTLGGTGKDKGKRHPTIGNNVTIGSGAKILGPISIGHNAKIGAGAVVVRCIPPNSTA
                     VGVPAHVVGREGIDETYVDLNHTDIPDPLEDEIRELEKRIRELERKLFSID"
     gene            33388..34194
                     /gene="pgeF"
                     /locus_tag="PKU88_03295"
     CDS             33388..34194
                     /gene="pgeF"
                     /locus_tag="PKU88_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPX68341.1"
                     /translation="MKDGLAYLTIPAFEKTGMVKHCFTTRIGGVSRGIYNSLNTSDDK
                     EDPVENVHRNLKLVCGAIGIDYSNLVFSDQTHEDNIRVVSIADLGKGISVPNDIKNTD
                     GLMTNIPGIPLITFYADCVPLFFLDKENKAIAVVHSGWKGTVQKIGVKTIRQMTEVYG
                     TKPEDCLAGIGPSIGPECFEVGQEVADQFTESFGTRPHIIKPLGNGKYMVDLWAANKL
                     MLMEMGVPENNITISGLCTKCREDLFFSYRRDKGRTGSMSAIMELKEEDT"
     gene            34194..34913
                     /locus_tag="PKU88_03300"
     CDS             34194..34913
                     /locus_tag="PKU88_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68342.1"
                     /translation="MNRKILIIEDEANIQELLRYNLEKNGYIVTISDNGIDGLQKALD
                     QVPDLILLDLMLPGLDGLEVCKRLRMQKATRRIPIFMLTAKSEELDKILGLELGADDY
                     ITKPFSIKELIARIRAAMRRLDDESSAVSDKAEKNKILKARDIEIDSEKYEVRKNNEK
                     ITLTLKEFELLKMLVENKGKVLTRDFLLDKIWGYEYMGDTRTVDVHIRHVRQKLNDDE
                     GSGRMIETIRGVGYRFISEGE"
     gene            34916..36685
                     /locus_tag="PKU88_03305"
     CDS             34916..36685
                     /locus_tag="PKU88_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX68343.1"
                     /translation="MQRKIFFNIVVVLLLGVIISGLLSARIVKKNLLSDIEKSLTTEA
                     ALVRELLGESLINNSNDIGIYVGKIKQTANSRITIVDVMGNVIIDTDKEYSEMENHAG
                     RPEIAAALKGEAESSIRYSSTLRVDFIYVAQPILKGSSIIGAVRLSKPLYEVKNMLNS
                     LYINVFIAVLIGIFVASVLGYKMAMNITKPIKEITYTASRIAKGQLDRRINITSRNEI
                     GILADSINDMASKLRDTITSLQDKNIKLESIMSSMINGIIAIDNSEKILFINHVAESL
                     LGITGGEITGKHLLQVVRNNSIDNYFKKMLKDKEFFNTEVNLNDSNEKILKFHANSIK
                     QNGENGIEGIIITIQDVTELRKLERVRTEFIANVSHELKTPLTSIKGFTETLKSFDFD
                     DKQDAIRFLNIIEDEADRLYRLINDILSLSELEQKKTKTVKEKIDIEKAVEEVLSVLK
                     SQSDKKNIELSAKIQEGLESISGDGDKFKQMLINLIDNAVKYTPENGKVSIEAYKQSH
                     NADLDKLIIKVKDNGIGIPKQHIPRLFERFYRVDKARSRTIGGTGLGLAIVKHIVMLF
                     NGEIEVTSEVGKGTEFRIILPMK"
     gene            36842..37696
                     /locus_tag="PKU88_03310"
     CDS             36842..37696
                     /locus_tag="PKU88_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX68344.1"
                     /translation="MRIKKTLAFICAAVLLIGALTGCAPAETNEPAANGLGESITING
                     STSVQPLAEELAKAFKEKNPDATIDIQGGGSGVGIKSAADGVADIGMSSRDLKPEEKS
                     LKEFKIAVDGIAVIVNPTNEIQGLSMEQILKIYTGEITDWKEVGGKEGKITVVTREEG
                     SGTRGAFIELTKVESKSEGDRTVASAITQGSTGAVITTVAGDPNAIGYASFGSAKENT
                     DIKLISVDGKTCTEENIYAGEYKISRPFLMLTQAEPTGLAKAFLDFILSEEGQTVVSG
                     HNYLKAVK"
     gene            37717..38565
                     /gene="pstC"
                     /locus_tag="PKU88_03315"
     CDS             37717..38565
                     /gene="pstC"
                     /locus_tag="PKU88_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPX68345.1"
                     /translation="MKAYERVIQLVLFISAIIAVFGVILISYFIISEGYPVIRKVGLL
                     DFLSGNRWAPTQGVYGIYPMIVGSLLVTAGALVLGVPIGIGCAIFLAELAKPRIAGIL
                     RPGIELLAGIPSVIYGLYGLAVIVPFIRKVFNNQGFSVLAGSIVLAVMILPTIINVSE
                     SAIRSVPRDYKEASLSLGSSHWQSIKNVIVPAARSGITAAVILGMGRAIGETMAVIMI
                     VGNSPLVPESILSPVRTLTGNIGIEMGYAGGEHQKALFATGIVLFVFIMLLNIAAHLI
                     PKKAGE"
     gene            38606..39424
                     /gene="pstA"
                     /locus_tag="PKU88_03320"
     CDS             38606..39424
                     /gene="pstA"
                     /locus_tag="PKU88_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPX68346.1"
                     /translation="MRKNEKTAFIFLWAAAAITVLTLIAIVGSVLINGIGHLSLDFLT
                     KEPENMGKDGGIFSIIITTIYLALFSLLIAAPIGISAAIFLTEYAKEGPVIRLIRFGT
                     ETLAGIPSIIFGLFGYVFFVVFLKFRYSMVSGGLTLTFMILPTIIRTAEESIKMVPMS
                     YREGSLALGATKWQTIYKVVLPASIPGILTGVILGIGRVVGETAAVIYTAGSSLGLPN
                     SIWRPGRTMAVHLYILASEGLSKSNMYATATVLIITVLIINFTANRLIKQFIRS"
     gene            39440..40192
                     /gene="pstB"
                     /locus_tag="PKU88_03325"
     CDS             39440..40192
                     /gene="pstB"
                     /locus_tag="PKU88_03325"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPX68347.1"
                     /translation="MAENKIEVSKLNLYYTDFHALKNVDINIAKNTVTAFIGPSGCGK
                     STFLRTLNRMNDLIDGVRISGKISLDGADIQKYDVIELRKRVGMVFQKPNPFPMSVYD
                     NVAYGPRVHGLRDRRKLDDIVEKSLKSAAIWDEVKDRLKKSALGLSGGQQQRLCIARV
                     LAVEPEVLLMDEPTSALDPGSTLKIEDLMEQLKKDYTVVIVTHNMQQAGRISDYTAFF
                     LNGEIVECDLTENVFYKPRDKRTEDYITGRFG"
     gene            40223..40879
                     /gene="phoU"
                     /locus_tag="PKU88_03330"
     CDS             40223..40879
                     /gene="phoU"
                     /locus_tag="PKU88_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPX68348.1"
                     /translation="MTRNYFDQELQELQVSLLKMSSMVEEILGNSVKALKAQDAELAQ
                     KVIDDDDEIDTMEYVVEDKCIKLIALQSPLARDLRVIATALKIITDLERIADYAVDIA
                     KITIKLQHEKYIKELIDIPRMAEIAVRMVKGAIDAYVHLDTSQAHEIAKLDDEVDGLY
                     KQIFRELLFIMMEDPRTVHQATYFLLISRYLERICDHVTNICERIIHTVTSEHVNLNE
                     "
     gene            40976..42319
                     /locus_tag="PKU88_03335"
     CDS             40976..42319
                     /locus_tag="PKU88_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HPX68349.1"
                     /translation="MGLYVREENNLANPGVVIMEVRPSSIAEELGIKKGDRLISINGA
                     RIKDILEYKYLTADEFLEVEIEHGNGEIWIYEVEKEYDEDIGIIFEGIIDRPKSCHNK
                     CIFCFIDQLPSGMRETLYFKDDDTRLSFLQGNFVTLTNLSEREIDRIIKYHISPINVS
                     VHTTDPGLRRKMLNNKNAGKLMDYLERLKEGKIEVKAQIVLCPGVNDGENLLKTLEDL
                     AGLHPQVSCVAVVPVGITKYRQGLYELAEYDGISAASVIRMVRKAQDKYLSELNTRFV
                     FLSDEFFLISGEELPGFEEYEEFTQLENGVGLIALFNREVEESLKKLGRYNKKTRLIS
                     IITGEYAAPNLKMAASKIESCIEGLKIRIFPINNDFFGPGVKVAGLLTGKDIMDQLKD
                     KKVGRDIFIPDCMLKKDEKIFLDDVTVEEMEKELGAKITICREDGSDLVKKIISV"
     gene            42347..43660
                     /gene="der"
                     /locus_tag="PKU88_03340"
     CDS             42347..43660
                     /gene="der"
                     /locus_tag="PKU88_03340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPX68350.1"
                     /translation="MAKSIVAIVGRPNVGKSTLFNKLAGKRISIVEDHPGVTRDRIYT
                     EVQWQDRAFTLIDTGGIEPHSEDLMLKQMKQQAEVAIETAEVIVFLVDGQEGITPSDR
                     EVANLLRRTRKKIILAVNKIDAPKYKDNIYEFYNLGLGEPMGISSGQALGLGDLLDEI
                     INNLLPEEEEVYDEYTIKVAVVGKPNVGKSSLVNKILGEQRVIVSDVPGTTRDAIDTP
                     FTVGEDKYVFIDTAGMRKRGKVFESIERYSVIRSLTAIERADVCLMLIDGKEGVSEQD
                     SKIAGYIHDQGKAAVIIVNKWDIVEKDDKTIDKYKADIRNELSFMEYAPILFVSALTG
                     QRVNKVLELVNHVSNQHAMRISTGMLNDVINEAVLTNQPTVSGGRRFKIYYATQVSIK
                     PPAFALFVNEPSLMHFSYQRYLENQIRKAFSFEGTPVRFLLRKKE"
     gene            43963..44991
                     /locus_tag="PKU88_03345"
     CDS             43963..44991
                     /locus_tag="PKU88_03345"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPX68351.1"
                     /translation="MSNNISIIGAGGWGTAVSVLLSGKGFNVKLWARDNELIEKMRDT
                     RENPAYLPGVTLKENILVSSDIEYCCSGSEIIIIAAPSHAMRDTCKKIKEFASKKQII
                     VSLSKGIENITLQRMSEIINEIMTENEVAVVSGPSHAEEVARGIPTAVVASSPKRAVA
                     EYIQDIFMSPLFRVYTNPDIIGVELGGALKNIIALGAGIIDGLELGDNTKAAVMTRGI
                     VEMARLGESLGACRSTFNGLSGIGDLIVTCTSMHSRNRRAGIAIGQGKSVEEVIRGTR
                     MVVEGIRTTKSAYLLAQRQKVEMPITQEIYNLLFNKTDIRNSVMNLTMRSKTHEMEEV
                     AENKVFEW"
     gene            45118..46596
                     /gene="spoIVA"
                     /locus_tag="PKU88_03350"
     CDS             45118..46596
                     /gene="spoIVA"
                     /locus_tag="PKU88_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HPX68352.1"
                     /translation="MEGYDVYQDISERTEGCIYIGVVGPVRTGKSTFIKRFMDLLVLP
                     NMDNEFKRERTRDELPQSANGKTIMTTEPKFVPNEPIELTLRENAKFKVRLVDCVGYL
                     VEGAMGHLEDNSPRMVNTPWFEDRIPFEEAAEIGTRKVIADHSTIGLVVTTDGSITDL
                     PRRSYIEAEERVIKELKELEKPFIVALNSARPKAQETIELREALETKYNTPVMIIDAL
                     KMEMEDLEGILGKILFEFPAREINFHIPRWMDTLELDHWLKQGIVDSIRTNLSEVNRI
                     RDIDDAIRKFDGAENLQAVEISDMRLGEGVVNITISLDDGLFYRVLGEVSGYSLEGDY
                     QLVALVKELAQSKREYDKVKSALGDVKEFGYGMVAPSVEEIKLEEPVIVKQGSKFGVR
                     LRASAPSLHIIKTDIETEIAPIVGTEKQSEEFIKYFMEEFENNPQKIWESNMFGKSLY
                     DMVKEELQGKLYKMPEDIQNKLQLTLQKVVNDTRGSLICIII"
     gene            46755..47438
                     /locus_tag="PKU88_03355"
     CDS             46755..47438
                     /locus_tag="PKU88_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HPX68353.1"
                     /translation="MMESIKRLRNPSELKIAAKENLDGKWLIAIAVTVVAWLLLDAFT
                     SSNGGNAAYKYIIENGKSIRVQADGSAFKNMMSIVSLILGGPIYFGVASYFLKLARYE
                     TAEFNDMFSGFSLFKTNFVLHLLMIVFTVLWALLLIVPGIIAAIKYSMAFYILNDNPE
                     IGAMEAIRRSKEMMEGHKMRFAEMWLSFLGWFLLGIVTFGLGMIYAVPYYRAAKANFY
                     LDLKESYSV"
     gene            complement(47479..47631)
                     /locus_tag="PKU88_03360"
     CDS             complement(47479..47631)
                     /locus_tag="PKU88_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68354.1"
                     /translation="MKHSKGVNAASSTGAVDESRNETTRIPYTDSISAPASDNIQKKH
                     KKNKKK"
     gene            47872..48804
                     /locus_tag="PKU88_03365"
     CDS             47872..48804
                     /locus_tag="PKU88_03365"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HPX68355.1"
                     /translation="MRCRRNEREKGDCSGRGAVYSLVNLIFGLVALTIKMTFKIVEVV
                     FQTLFGFISLFVSGSRDSQKTPNVYEKPMPEKNEAPSNVREFKKPESSKAQADVPEVE
                     SSDAPEEVKNKDFYVVLFILTIIPVIVALAMGKLYYAGIIGAAGFGLMMLVGIAASAY
                     GKGAAKETCEDEEEAEEDDSVERLIKDAFEKLFTIRKDIGKIQDADIRARLEAICCTG
                     EKIIGEVRTNPEGLAHVRKFFYYYIDAFGEIVNKYLKLSSFEESSEEIGRLMAETERS
                     FADIDGIFKDLCEKLLEKDMMHLKAEINVIKNSN"
     regulatory      48858..48988
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            49122..50213
                     /gene="ribD"
                     /locus_tag="PKU88_03370"
     CDS             49122..50213
                     /gene="ribD"
                     /locus_tag="PKU88_03370"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPX68356.1"
                     /translation="MKRALELAARGSGRTSPNPLVGAVIVKNGRIIGEGWHERAGEPH
                     AEINAIMNSRESVEGATIYVNLEPCSHFGKTPPCAEELVKRKFKRVVMAMEDPNPLVA
                     GRGIKLLRESGIQADVGVLNAEALKLNDIFIKHITVKEPFVLLKTAMTLDGKAASRTG
                     DSKWISGESSRAYVHSLRNRYSSILVGVNTVISDDPELTSRIDGVQTRNPVRIIMDSG
                     GRIPIDANVLNIDESRRTIIAATEDMTEEKRRQLESKGAEILITDKRNGRVDIRQLLE
                     ELAGRGIDSLMIEGGGTVAAAFLEEGLIDKVAFFIAPVVIGGKTAPTPLMGEGVSLIE
                     KGYRLKHRSISVFGQDVLIEGYINVPWER"
     gene            50220..50864
                     /locus_tag="PKU88_03375"
     CDS             50220..50864
                     /locus_tag="PKU88_03375"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPX68357.1"
                     /translation="MFTGIVEEIGRIRNIRKGEKSESLIVECKNILEGTKMGDSISVN
                     GVCLTVADMSANSFEADVMPETVRATNLKYLKPGDSVNLERALSPTGRFGGHIVSGHV
                     DGTGTIQEIKREDNAIWFTISARNEILKYVIHKGSVAVDGISLTVQYADERVFKIALI
                     PHTRDYTTLPGRKVGYCVNIECDIIGKYIEKLMNYTGKDSKGGISLEYLLENGF"
     gene            50876..52072
                     /locus_tag="PKU88_03380"
     CDS             50876..52072
                     /locus_tag="PKU88_03380"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPX68358.1"
                     /translation="MFCRIEDALDELKKGNMIIVVDDEDRENEGDLLMAAEKVTPEAI
                     NFMAMHGRGLICVPMTEERLKELKIEQMTGNSTDLRETAFTVSVDIKSAATGISAYER
                     ADTVRALVNPDTKPEELAKPGHIFPLKARKGGVLKRAGHTEAAVDLAGMAGFYPAGVI
                     CEIMKDDGTMARLFDLFEYAKKHKLKMITIADLIKYRSREEKLVYKAADALLPTKYGE
                     FRVMAYESKINGEHHIALVKGDVAAEEPVLVRVHSECLTGDAFGSVRCDCGEQLAEAA
                     AMIQDEGRGVLLYMRQEGRGIGLVNKIKAYELQDTGIDTVEANELLGFPADLRDYGIG
                     AQILKDLGIKKIRLITNNPKKIKGLAGYGLEVVERVPIEMDANIRNEKYLLTKKEKMG
                     HMFHNI"
     gene            52069..52551
                     /gene="ribE"
                     /locus_tag="PKU88_03385"
     CDS             52069..52551
                     /gene="ribE"
                     /locus_tag="PKU88_03385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPX68359.1"
                     /translation="MRGGKKLRVYEGNLTAGDRRFAVIAGRFNEFISSKLLGGCIDGL
                     KRHGCPESNIEVSWVPGAFEIPLAAQKLARTGRYDAVICLGAVIRGSTPHFDYVCSEV
                     SKGIAKVMLDTGLPIIFGVLTTESIEQAVERAGTKSGNKGFDAAMTAIEMANLLSTIG
                     "
     gene            complement(53163..54278)
                     /locus_tag="PKU88_03390"
     CDS             complement(53163..54278)
                     /locus_tag="PKU88_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008910026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPX68360.1"
                     /translation="MKDPRIKQMAINLLNYSVQLKPNENILIEVFDSGEELASELVKE
                     IYKIGGKPFLTIKNRSLMRELILGTDAEHMSRTAHYEAERMKDMQAYIGIRGAYNSSE
                     WADIPSEKLGFYQSFWSKTVHTNIRVAKTKWCIMRFPNSSMAQMANMSKEQFEDFYFD
                     VCNLDYSKMSKAMDPLIELMNNTDRVRIIGTGTDLSFSIKGLPAIKCDGKSNIPDGEV
                     YSAPVRDSVNGYITYNTPSEYQGYTYENIRLEFKDGRIINASANDTDRINQVFDTDEG
                     ARYVGEFALGVNPYIIKPMKDILFDEKIMGSFHFTPGSAYDHCFNGNNSAIHWDLVNI
                     QTPEYGGGEIWFDDILVRKDGRFILPELDVLNPENLK"
     gene            54549..55250
                     /locus_tag="PKU88_03395"
     CDS             54549..55250
                     /locus_tag="PKU88_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HPX68361.1"
                     /translation="MSVIEHAYGKTFKTILWAVNPLKKVFKKTLCEVHVFINEKAVEI
                     LRNDGFQDAYEFFSINKGCLNDGAVWADQDFKSREHFYNPFTHRGLYGCKSSKHLFRK
                     YYGCALVYWDCGDKDKAMFYLGAAVHLVQDATIPQHGSVKLLKSHRKYEQWIHKVHDD
                     FLHYSVSEGGIYLENPFEYIEKNAKEALEAYTKYSLIRNPSEKFFRIANRTFPLAQKT
                     TAGCLMNFYAKINEG"
     gene            complement(55237..57006)
                     /locus_tag="PKU88_03400"
     CDS             complement(55237..57006)
                     /locus_tag="PKU88_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPX68362.1"
                     /translation="MKNGILLCAINAKYIHSNLAVRYLKKFCEAHIKGIDIAEFSIND
                     SISSILKKLYCSNARIYGFSCYIWNISMVLDICSSLKKARPEAVIILGGPEVSYDAAD
                     LLSKYSFIEYIVIGEGEAAMLDLLCFLTAGKSNLPDIPGIAFRSGESVAITEPRPDIP
                     DLDMLPFPYDSFDGLDNRIIYYETSRGCPFNCQYCLSSTIRGVRYLAMDRVRQDIKSF
                     ANAGIKQVKLVDRTFNCDLERSIEIMQYIIDLNPSTNFHFEIAADLIDVRFLEIAGKA
                     PDGMFQFEIGVQSTNPKTLSEIKRKMDFDKVSHNVARLLSFKNAHIHLDLIAGLPYED
                     MESFEKSFNDVYSLNPDMLQLGFLKLLKGSGIRQGCKKYRIQHHDFPPYEVISTAWLS
                     FKEIMELKDIENVLEQYYNSGRFRHTLDFLLQAAETEPFDFYRRLSGYWNQKGHFNAP
                     KNVNELYTVLKCFAEDAFVNRLDNEQFGLLNEYMKLDWLLYVRSGSMPAAINRFNHAA
                     IKEEIHGYIKNKLTDMDTFKDFKNLSLREILKQVGYEVFLRNIFADTPSQDEIVMFFP
                     LRPSSSKKIINFAAVPLKEFTLH"
     gene            57154..57798
                     /gene="nth"
                     /locus_tag="PKU88_03405"
     CDS             57154..57798
                     /gene="nth"
                     /locus_tag="PKU88_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPX68363.1"
                     /translation="MRDNDDLKSRAKKISEILFKIYPEATCSLDYDKPLELLIATMLA
                     AQCTDERVNIVTRDLFKKYTSVYNYASANPEELEEDIRPTGFYRNKANNIIGCCKKLI
                     KDFGGKVPDNMEDLLSLSGVGRKTANVVLGNIYKIPGIIVDTHCKRLSNRIGLTKEED
                     PEKIEFDLMEILPRENWTAFSNSLVYHGRAICNARKPKCVQCHIAQYCDFFNNI"
     gene            58004..58330
                     /locus_tag="PKU88_03410"
     CDS             58004..58330
                     /locus_tag="PKU88_03410"
                     /inference="COORDINATES: protein motif:HMM:NF028309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HPX68364.1"
                     /translation="MTTDIIKGIRETELAAEEKIKAAQQKAKDILMSAEQEADHIIKA
                     AVDQAIQESKKQLEVAEKEANLEAESKRKQNDELCQELRRKAEEKMEEAVNLVMERIV
                     NMNGNS"
     gene            58317..60233
                     /locus_tag="PKU88_03415"
     CDS             58317..60233
                     /locus_tag="PKU88_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HPX68365.1"
                     /translation="MAIVKMKKATMIALQSEKNEILNDLQRFGYLQVVNLEERMEQGF
                     EELQPDSDNEAVGRLEAKLSQVKYSIDFLGKFNTAKKPLFAQKVQVDETKRKEYLDNE
                     EKLNEIYESCRSIDSRFTEIKSSETKLNNAIAQLTPWLALKEKLEEIGTTHNTNSILG
                     FIASKYEDELSTAVKDEDLTAYLEKVGVEKENSYFMVIYHQTCEERISQLLKQVGWTK
                     VTFSEYSGTPEENINRIKAEISNLENIKKELAKKAEAMVPESGFLEILYDLLSIEKDR
                     RSVVKDFIKTDKTFMLQGWIPEKFADDMTKLLDGITDKYTISFEDPSEEDEIPVLLNN
                     PKAVKPYEMITDLYSVPNAKGIDPNIFMAPFFVVFFGIMVTDAVYGILMSALTGYVLY
                     KYKPQGGMKNMMWIMFFGGISTFFWGGIFGGWLGDLIKVKPWWFNPLDEPLKMLIFCL
                     ILGVIHLYTGYILQAYQNIKAGNFMDAIYDQVLWLMLLTGLMFLALPPLAAVGKYMAI
                     VGAAGTVIFSARSEKNIFKRLISGVLALYNVSGFLGDVLSYSRLFALCLATGVIAQVF
                     NAMGLMMGGSIIGKIIMIAFLAFAHVFNTALGVLGAYVHTSRLQYVEFFGKFYEGEGK
                     PFNPLRIRTKYIQE"
     gene            60301..60774
                     /locus_tag="PKU88_03420"
     CDS             60301..60774
                     /locus_tag="PKU88_03420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPX68366.1"
                     /translation="MGNSGLWMAFLGSALAAGLAGCGSAIGVGIAGQAAAGVITEDPS
                     KFGKTLLLQALPGTQGIYGLLIGFLIWSKIGVFGTIVPVTIEQGLMLMLVGLPVGLVG
                     LISAIYQGKASAASCGIIAKRPEEFGKGMIYPAMVETYAVLSLLASFLMLNGIKL"
     assembly_gap    60824..60923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            60943..61545
                     /locus_tag="PKU88_03425"
     CDS             60943..61545
                     /locus_tag="PKU88_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPX68367.1"
                     /translation="MAGVENIKEKILQDADLRAKEILEKARLQANEIVEKAKQKAASR
                     AKEISQKSVYDVSEKKRIINSIVELEMRKDVLAAKQKAIGEVFDKVLERMNNLEDSRY
                     EKVIIDMLAASAESGEEEIVFSESGRKKLSSDFLNKLNKTLAAAGKKGNLKISDETRN
                     ISGGFILKSQGVEINNSFEAIIRLYRDEIEPKVAEIFFRA"
     gene            61591..62592
                     /locus_tag="PKU88_03430"
     CDS             61591..62592
                     /locus_tag="PKU88_03430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HPX68368.1"
                     /translation="MSENKYLYSVTRIRALETKLLDKAKIERMIEARNADEIIKILYE
                     TEYANSISEMKSVEDYESVLSKELSNTYKLLSEISPVPELTNLFQLRYDIHNLKTLLK
                     SSYLDEENDELLSEIGTIPIQQLKGMIKEKVFSDLDPLVRESLEEVVGEFTVNPDPQL
                     IDVTLDKALYKLMYKAAKDNKSSFLTDYISTQIDLINIKSLIRVKSMGYGREFLKKVI
                     LENGKLDYAFFSDIFDESLETLIDRLAYKDYGKVVEEGISNYIKTKSLTKFEKLSDDF
                     IFELAKKGKFVAFGIEPLIGYLMAKENETKIIRMIMVGKINEIPNELIRERLRDVYV"
     gene            62585..62908
                     /locus_tag="PKU88_03435"
     CDS             62585..62908
                     /locus_tag="PKU88_03435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPX68369.1"
                     /translation="MYKIGVIGDKDSILGFKALGISVFPTNDIREANRLIHKLARENY
                     AVIFVTEQLAKDLDEAIGEYKNSLTPAIIMIPNNRGTLGLGIDGVKKSVEKAIGSDIL
                     FGKEG"
     gene            62913..64685
                     /locus_tag="PKU88_03440"
     CDS             62913..64685
                     /locus_tag="PKU88_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPX68370.1"
                     /translation="MSDGRIIKVSGPLVIAEGMLDAKMYDVVRVSDKNLIGEIIEIRG
                     DKASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLIESIFDGIQRPLDVVRSASGDHI
                     ARGVQVEKLNKTRKWNFEPSVKQGDAVKTGDIIGIVKETSIVVHKIMVPHGIEGTIEE
                     IYSGEFTILETVAKIKTKDGIKEVQMLQKWPVRKGRPYKEKMSPSTPMTTGQRIIDTF
                     FPVAKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYIGCGERGNEMTDVLMEFPELK
                     DPKTGEPLMKRTVLIANTSDMPVAAREASIYTGITIGEYFRDMGYSVALMADSTSRWA
                     EALREMSGRLEEMPGEEGYPAYLGSRAAEFYERAGKVVCVGTEDREGALTAVGAVSPP
                     GGDLSEPVTQATLRIVKVFWGLDAALAYRRHFPAINWLQSYSLYLDRLGDWFTEEVAL
                     DWNNLRMETMRLLQEEAELNEIVRLVGFDALSLKDRFTLEASKSIREDYLHQNAFHEI
                     DTYTSIHKQYRMLKAILLFYHEGQKALSEGAFYKELTTLPVREDIAKAKLILENDYES
                     FDVLEANIRDQIASILSKGGKIVC"
     gene            64679..66058
                     /locus_tag="PKU88_03445"
     CDS             64679..66058
                     /locus_tag="PKU88_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPX68371.1"
                     /translation="MLKEYKTIKEVAGPLMFVDQVEGVKYDELVEIELKNGEMRRGKV
                     LEVSGSTALVQLFEGSTGININEGKVRFTGHSLELPVSTDMLGRVFDGLGRPKDGGPK
                     IIPESRKDINGNPINPAARDYPSEFIQTGISAIDGLNTLVRGQKLPVFSGSGLPHARL
                     AAQIARQAKVLGTESKFAVVFAAIGITFEDADYFVSDFKRTGAIDRAVLFMNLANDPA
                     IERIATPRMALTAAEYLAYEKDMHVLVIMTDITNYCDALREVSAARKEVPGRRGYPGY
                     LYTDLATIYERAGRIRDRKGSITQIPILSMPEDDKTHPIPDLTGYITEGQIILSRELY
                     RKGIMPPIDVLPSLSRLKDKGIGRGKTREDHADTMNQLFASYARGKEAKELAVILGEG
                     ALSDTDKLFAKFADEFENKYVSQGDSEDRSIEQTLTLGWELLSILPKAELKRIKDEYI
                     DKYLPKGDA"
     gene            66062..>66168
                     /locus_tag="PKU88_03450"
     CDS             66062..>66168
                     /locus_tag="PKU88_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPX68372.1"
                     /translation="MAKLNVNPTRMELTGLKGRLKTAKRGHKLLKDKQD"
     assembly_gap    66169..66268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <66269..66579
                     /locus_tag="PKU88_03455"
     CDS             <66269..66579
                     /locus_tag="PKU88_03455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPX68373.1"
                     /translation="EASSIYPYGFAATSGELDGAIFSLQEVFDKLIELAQVEKTCQLM
                     ADEIEKTRRRVNALEYVMIPQLIETVKYITMKLDENERGSRVRLMKVKDMMEKQKAEA
                     "
     gene            complement(66613..66819)
                     /locus_tag="PKU88_03460"
     CDS             complement(66613..66819)
                     /locus_tag="PKU88_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HPX68374.1"
                     /translation="MHKNISSNDISSNKMHAHNHSIKCNVDTCSYYDNNYCSASVIEV
                     NPMGDGVAHSSEGTCCTTFVEAKQ"
     gene            67092..68270
                     /locus_tag="PKU88_03465"
     CDS             67092..68270
                     /locus_tag="PKU88_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPX68375.1"
                     /translation="MGIQFANRMDNLKGSVIRELLKYAEQPDIISFAGGLPAPELFPV
                     EGMMKATERVMMEDGKASLQYGATDGYLPLRKKIAERSKKIGFIPDPSEILITSGSQQ
                     GLDFTAKIFINKDDVIITESPSYLGALNAFKAYQPKFVEIPMDEDGMIIEELEKALAA
                     NKNVKFIYTIPDFQNPTGRTMPVERRKKLMELATEYEIPVIEDNPYGELRFEGEILPS
                     LKCFDPKNLVILLGTFSKILAPGMRLGWVIARPDLLIKYNLVKQGADLQSSSMAQRQA
                     NMFMEMYDIEKHIEKIIKVYSARRNLMLDTIKKEFPSNVKCTNPQGGLFAWATFPEEV
                     DASVILKKALEQKVAFVPGEPFYPNGGNANHCRLNYSCMSEDKIVEGIARLGKTLKSM
                     "
     gene            68480..68638
                     /locus_tag="PKU88_03470"
     CDS             68480..68638
                     /locus_tag="PKU88_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPX68376.1"
                     /translation="MEKWTCTACGYVYDPEAGDPDNGIEPGTPFEDIPEDWVCPLCGV
                     GKDMFEKE"
     gene            68785..68858
                     /locus_tag="PKU88_03475"
     tRNA            68785..68858
                     /locus_tag="PKU88_03475"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68817..68819,aa:Gln,seq:ctg)
     gene            complement(68994..69422)
                     /locus_tag="PKU88_03480"
     CDS             complement(68994..69422)
                     /locus_tag="PKU88_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPX68377.1"
                     /translation="MDKWGHYTPKTESGIRQIPLLESMVKVLKTHKAEQAKEKLLAGP
                     LYNDNDLVFCTNLGKEIDPPNFSKVFYRILKKAGIAHANLHCLRHTFATRGLENGIEL
                     KVMQVLLGHSSIVITADTYSHVLPNKKKEAMEKLNNIFDI"
BASE COUNT        21853 a        12305 c        16468 g        18909 t
ORIGIN      
        1 aagcttgcca ttgcagcatt tctgattcca tatatatttg ttattaatcc tcaattactg
       61 ttgataaata caacaggact ggaagtgatt cagattacaa tcacatcctt aataggtatg
      121 tttggaatag gaatagccat ggaaggcttc atattcggaa aggtaaatcc tttgatcaga
      181 ttaattttca tggccggcgg gctttgcctt attgacccgg gtgtatatac cgacatggca
      241 ggcattatag caatcggact gggattggca tatcagtggt ttaagtataa aagaactaca
      301 gcaaaaagta tttagccgga ataataagga agagttgatt aagatatcac tcttcctttt
      361 caaattcttc caaatacttt gttttatctg cttcaaccag cggaagtact ctctgaagtt
      421 cagccttatg gatctcaaga agttcattaa taatgctctc catttcactg cgcttttcat
      481 tgtattttag cttgacaatc catctggggt tgtattctcc tgacccgtcc agaagatcaa
      541 tattgaattt gctgaatacc tcttcattgt ataagtcata tattccattg tattgataat
      601 cctcaacatc ggctgtagtg aggacaagaa atatgaagta ttctccctct tcctcagaga
      661 ggtatgcatt ctctatcagc tcaaggcccg aatcaatttt ataagaatct atcgtttgga
      721 taaaaaaacc tgttgcccta ttcttttcca taagcacaag tatgttgtat ttgtccattt
      781 tattacctcc ggggcatact gcctggcatc ttgcagaata aatatggtat aattcaataa
      841 ttaaatgtga ttatatttta accgtttcag gcacaactta caaggagtat aaaaatgaaa
      901 attatcaata aaaccattaa tggaagattt gtgggcagat tgaacaggtt tgagggaata
      961 gcggagataa atggagggga agtaccggtc catataccta ataccggccg atgcagggaa
     1021 ctcctgttta aaggagccgg aatcatactg gaaataaggg agagcaagac aaggaagacc
     1081 ccctatgagc tgataatggt atataaggga gacaggctta tatcaattga ttcccaggcg
     1141 ccgaacaaga ttgttgagga agctgtaaga ggaggactga tagaggagat agggagctac
     1201 gattatgtca agagggaagc cttttatggg caaagcagat ttgatctgct tttaaagaaa
     1261 accgaaggaa gcggaaaagc tgactcctgt tatatggaag taaagggagt aactcttgag
     1321 attaacggca ccgcattgtt tcccgatgct cccactgaaa gaggtgcaag gcatatgaag
     1381 gaattggctg ctgcacgtaa ggagggttac agagccgtcg tcattttcct cgttcagatg
     1441 gagggtatca aatgctttac tcccaacaag ctgatggact ccggatttgc cgaagccctg
     1501 gcagatgcac attccgaagg ggtggaagtg ctttcataca attgcagggt atcggagaag
     1561 gaaatagtga ttaatgaaag agttgaagta gtattgtgaa gcaatgatga aaaattccga
     1621 aatatataca agttttaaga gatgtttttg ttcattatcg aaatttaata taatatgaac
     1681 tttttttaat aaatttcata ttatatatat tgaaatgaag atttcatagg gctataataa
     1741 aaatgacaat tgaatcttcg ttaggtgagg ctcctatacg gataaatgct gctacccgga
     1801 aacatcgaga gatgccagcg ggttaacaag tattatcgaa ttaaggttct acttaatgca
     1861 gctggaaaac ttctatccaa agctgtatag tgccaaagct ccgcgagtga agaaatgtaa
     1921 tgctgttaca gtatactatt cctttcactc cagtgaaagg attttgtttt tgttagaagc
     1981 ttgttaagat ttatatgcta agcagtaaat aatacaacgg caaattttaa taagatattc
     2041 tatattttgc ttcactgcac gtggataccc acgagcagcc ggcaaaaaga atatctaatt
     2101 aaaattagcc tacggtgagg tgatttaaat ggacgctggg cccagtaatt attgcagtag
     2161 aatatggaaa gactataggg gaaagtctgt ttatttcacc aatgacagcc gcttttccca
     2221 ttgttgggag gaaagactat ggaaaacatc attaaggatt atattgaagt aaagaattat
     2281 tcagccataa aaaaagcgct ggaggatatg ctgccggccg atattgcaga gcttcttgaa
     2341 gaacttgata caaagaacag ccttctggtt ttcaggatgc tccataagga tgtcgcagta
     2401 gaagtatttg cacacctttc tgtaaaacag cagaccagtt ttacccttat gataaaggag
     2461 gacgagttaa aagaaatatt ggaggagctg tatttcgacg ataagattga ctaccttgag
     2521 gaaatgccct caagtgttgt aaaaaagata ctgaagtatt ctccggaaac agagagaaaa
     2581 cttatcaacc agttcttgaa ttatccggag aactcagcgg gaagcctgat gacgatagag
     2641 ctggttgacc tgaagaagga gcttaccatt gatgaagcgc tgctgcgtat caggcggaca
     2701 gctttggata aggaaaccat atatacctgc tacgtaacgg acagtaacag aaagctggag
     2761 ggtacagtct cccttaagga cctggtaatt gcacccggga ataaaaaagt tgaagatatc
     2821 atggagacgg atcccatatt cgtaaggact atcgacgacc aggaagaggt ggcgaatctc
     2881 tttaagaaat atgacctttt gtccgtaccg gttgtggatc aggaaaacag gctgatagga
     2941 atcattacca tcgacgatat cgtggacgta attgaagaag aggcatcaga ggatatacag
     3001 atcatgcacg gtttggcacc cagtgaagaa gaatatctga agactgatat aataaccctg
     3061 gcaaagcaga gaattttctg gctgctgttc ctgatgcttt cggcaactat taccggaaac
     3121 ataatagaaa gctttcaggg cagcctggag aaggcagtag ggcttgcagt attcatacct
     3181 atgcttatgg gtaccggagg aaatgcgggg acacaatcat caacactggt aataagaggt
     3241 atcgctcttg gagaagttgt cttaagcgat atatcgaagg taatatggag agaactcaga
     3301 ataagcctga tggttggaat ttcgctggca gtagtgaatt ttgtacgggt gtattatctt
     3361 ggagggaact cttttacagt atctgtggtg gtaagcgtca cactcatact tacaattatg
     3421 atagcaaagg taataggagg cgtactgccg ctgattgcca aagcaattaa tattgacccg
     3481 gcaataatgg ccagcccatt gatttcaact attgtggatg cacttacact gctttcctat
     3541 tttaaaatag cggaagcgat tatgcatata tgaaacagac actgagaacg ggtaacacag
     3601 agaacaccga ttttgcactg aattcacggc aaattaatat aatttccgcc gtgcattcag
     3661 tggatttctg tgatttcagt gttatccgtt atttatctta ggctaagtgc ctttttcagt
     3721 atttttgagg ctatgggtgc agctgcactg ccgcctccgc cgccattttc aataagtacc
     3781 gcaattgcag ccttcggact gtctgccggg gcaaagccta caaaccatgc gtgggatttt
     3841 tgattgccgc tgacctcggc agtgcctgtt tttcccgcaa cggcaacttt gctgacctta
     3901 gccctggttc ccgtgccgga ctcaaccact tcgaccatca tatcttttat ggcttcagcg
     3961 gtgtttttat ccattatcct gcctatctgc ttcggtctga attctttgat tatcttattt
     4021 tcactgccgc tgatactgct tactacatat ggctgcatca taaccccatc atttgcaatg
     4081 gccgctgcca tcattgcagc atgaaggggc gtcataagca cctcaccctg tcctatagac
     4141 tgctgggcaa tgctcctgtt gtcagattta ctggggaaaa cgctttcgga tactcttata
     4201 tctgtctgta ttttcttgtt taacctgaac cttgacgctt cattgtacag attgctgttt
     4261 cccagcttaa gcccttgctt tatgaaatat gaattgcagg agtgtttcaa ggcctccgaa
     4321 agggttatgc tgccgtgagc cttgccgtcg ctgtctttga taatataacc gtccgcattt
     4381 acttctcccg tgcattccac ctcaatgtcc tgcaaattgt ttttcagtat tgaggaagcc
     4441 actgccagtt tgaatatcga acccggaggg taaagcccct ctgaagctct gttcagcaga
     4501 gggcttttct tatcatttat aaggctttcc cagttgtttt gtatgccgga ggggtcaaaa
     4561 tccggtttgc ttaccaaggc aagtatttct ccggtatccg gcttcagtac taccaccgct
     4621 ccttttttgc cccgcagcaa ttcggaggca taaacctgaa gggaacgatc cagtgtcaga
     4681 ttgatattgt tgccttttat ataataatca ttgattttgc ttctcaagtc ctccatagtg
     4741 tcgctggcgc tcataagctc cttgttataa agggcttcga ggcccgatga accatatttg
     4801 gcacttgaat atccaataac atgggaaaag tatctgggat agacatatgt gcgtttaact
     4861 ccggctttat acttttcact ctttgccaga atctccccgt ttttatccag tatgcttcct
     4921 cttattatat ttccctccga ttccctcaac cttctgttat atgagttgga agctatcaat
     4981 ctggactcaa acaggctgaa atacagaaga tacaagcata taagcgcaaa taaggacaag
     5041 tatatatatt taataatttt aattctgtct ttcatacaaa tccctcctat cggctcgcgg
     5101 tgcgcggcac gaggctctcg gcttcagatg tctgtgaagc tttgtctgtg cttatataat
     5161 acaatattcc cacattcatg aactgtgaaa gcagtgaact tccgccgtag cttacaaagg
     5221 gaagtgtgac acctgttatg ggtattatgt tcagtacacc gcatactatt acaagggttt
     5281 gaattgcggt aagggaggta aggcccaaag ctgcagatga atatatgggg ttgctgcatt
     5341 ttgagcttat atccataccg attatgctga gcagcgcata catggctaac agggatatcc
     5401 cattcaatat acctgtttcc tcacataatg cggaaaatat gaaatcggtg tggacagcag
     5461 gtatgaattt tgggtctccg agatacagac cagtaccgat gaagccgcct gtagcaactg
     5521 caaacagtga ctgtgttatc tgataggatt tgccggatat atatttccag ggatttatcc
     5581 atgccaaaac ccgggatctt atatatgaaa aagcgaagta gctaataaaa gcccctccta
     5641 atgctgcacc ggatgaggct gcagtatata agcccctaac atccctggag aaaagcatga
     5701 caaggtatac cgtgaaaaat aagaaggcgg agcctatatc cctttgaagg acaaaaaacg
     5761 ctgtaacggt aagcatagat gcggcaattc cggcaaatct ttttctgctt ggttcccggg
     5821 acagcagaca ggctgtgaag aacacaaaaa tcagcttgat gaattcggag ggttgtatat
     5881 tcgtgaaata aagatttatc caattttttg agccgttgat ttctataccc catatctgtg
     5941 ttgttaacag aagggcaaga atcaaacctc caaggagcaa atgatgctta tactttactt
     6001 caaatctttt cataaaaata aaggaagcaa aaaatccaag tatgcctgcg gctgcaatat
     6061 atgcctgttt tgtaccgtat tccgggctta acctgtacag catggcgagc cctgtctgaa
     6121 gcatattaca gacaacgtca aacataaggc aattgccgtc atttaaaagt tttgcaagaa
     6181 tcatccttga tatcatataa gcagcggcaa ggccgccgca gatatacaaa gccatatagt
     6241 ccaggggttc ggaataatat gaaatcagag cgaagttcat tgcaataaga accaaattca
     6301 gcaatgggga aacatacctg catatacgaa tattcttata gtatgtcatc cagcacgaca
     6361 aagcaaagcc ggcagttata agcgtatata tgaatctgta gctccatgaa aaaacttcaa
     6421 acacaatatc accggtttct ggtaaaattc caacaataat atattataca ataatattgg
     6481 gcaaaattca cctgcgcatt cacatatact ccaatatatg ctagaataat ctcattgaat
     6541 taagatgtaa aggagcataa tatgggttat aaatatgtat tattcgatct tgacggtact
     6601 ctgattaata caaacaggct gattttagat tcctttaagt atacttataa aaaatgcctc
     6661 ggcttggaag tctgcgagga ggatatactg aaatgcttcg gggagcctct gaaagtgaca
     6721 ttgaggagat actccgagga aaaggcggat gaattgttta atacatatat tgagtataat
     6781 gagtcgaggc ataacgatac agtgactata ttcgacggga ttcaggagct tttatccgaa
     6841 ttggtgaatc aaggccgtac ccttgccctt gttaccgcca aaaggagaag agtagcgatt
     6901 atgggccttg agttatttga tataaagaaa tactttgatg taatcgtggc acttgaagat
     6961 acggaactgc ataagccaaa cccggctccg gtgataaagg ccctggagct tttgggagcg
     7021 aagccctcgg aaacattaat ggtcggagac agtgtttttg atattcactg tgcccatgga
     7081 gcttctgttg cggccgctct ggtgaaatgg agcgctgccc gggacttcca ggatgatgct
     7141 tcatcagcgg attatgtagt tcatgacaca aaggagctgt tagatattat tatagggtag
     7201 cactttgaag ttagtggaag gcagagtacc gacgtgggga agatatcttc gacgaaacct
     7261 tccacctgac cccggaacga gaatacccca gaggaaaagc tagtctcacg acgcttttcc
     7321 gaccctaggg aaccctatgg ttcccttcga cggatgcaag catcctgagc accctccttt
     7381 atcggcaggg ggcttctcct ccttgcatct cccgctttgg aaggtgagga gaacatactt
     7441 cccttacagc cggagtactt tacatactat tctattattc tgctacagtc taattagcgc
     7501 atttttacaa acttcttctc aatccacgac actgacagat acataaacgc cgcagccaca
     7561 gccagtataa tgacgctggt catgaccagg tccatcttga atacctggcc gccatagatt
     7621 atcagatagc ccaagcctgc ctttgataca aggaattctc cgacaattac gcctaccagt
     7681 gacagcccta catttatctt cagcgttgaa attatctccg gaatactgga tgggaatacc
     7741 acttttctca gtatttgaac ctttgtggcg ccaaaggttt ttaagagctt gattttatcc
     7801 tcgtcacagc ttctgaagga ctgatagaca ttgagaatgg tgacaataat agacaagaga
     7861 agggccatta ctatgattga gccggtggtt ccgccgatcc atacgagtat aatcgggccc
     7921 aaagctgttt ttggcagtgc attaagcact accaggtatg ggtcaaggac tcttgccgta
     7981 aagttagacc accaaaggac aattgcagca agtgtcccca aaactgtgcc ggatacaaaa
     8041 ccaattatgg tttcatatat ggtgactccg gtatgcagaa acagggagcc gtccttagag
     8101 agatttatga cagtatttac aatcctggag ggctggctca tgatgaaggg gtctatccac
     8161 ttcatattcg cggcaatttc ccataatgca aatatcgctg aaagtattat tatcctggaa
     8221 atagtcacgg cattttttgt aattctttct tttctgatat attccctatg ttcattggaa
     8281 tattcttcat acatgaacat ccagctcctt ccatatctta ttgaaatata tcctgaattc
     8341 aggagcttct ctgcagataa taggagtctt gttttcgcag gtagtcagct gaataacatg
     8401 ctccgattta acttcccccg gcctccttgt aagcacaatt atcctgtctg acattgatac
     8461 tgcttctgct atgtcatgag taacaagaat cgctgtcttt ttctcctttt ttataataag
     8521 ccagatttct tcagaaatag caagtcttgt ctgatagtcg agggacgaaa agggttcgtc
     8581 cagaagcagt atttccggat tgacagccaa ggttcgtatc agagccgccc tttgacgcat
     8641 tcctcctgag agctgattgg gataataata cttgaattca ccgaggccat actcttccag
     8701 caatctttca gcatttctca ttgtatcctt gtttagtttc ttttgaattt caagtcctat
     8761 ggtagtattc ctgagtattg tgagccattc aaacagatga tccctctgcg gcatatatcc
     8821 gacattgctt gccaaccctt ttacaggctc attattaatt aaaatctcac cagtggaaga
     8881 ttttatgagc cccgaaataa gagaaagcaa agtagatttg ccgcagccgc tggggccgac
     8941 aatgctgacg aattctcctc ttcttatgtc cagagataga tttttaatgg cctgggtttc
     9001 accgtcaagt gtatggtagt tcatggtcac gttttttagc tctactacct tatccactgc
     9061 tacaccccca gtaagttctt ataactccat actattcatc agcagtggta ttagtgaata
     9121 aaaagaatca ttgacaggta tggtacagtt gctttttatt taagtgtcta tataaataca
     9181 tttttgtggt aaaatatttc gtaggtgcga ggcacgagcc gcgaggttag cgaagcgagg
     9241 tgaggggaat aatgtacaac accatagatt atgaacagct tgaaggggat tatgtgcttg
     9301 ttgatgtaag gagtccgaaa gagtattcgg aatttacaat accaggagct gtcaatctgc
     9361 cgatttttaa tgatgaagag agagaactga taggtacggt atacaactgt ggaagcatag
     9421 atgaggcaaa aaaaatggga gtggatttcg cttcaaaaaa gcttcctctt ttgtatgaag
     9481 aaataaaaag gctcagggaa ggccatgaca aggtaatact gttctgtgag cgcggagggt
     9541 atagaagcag ttccctgtgt gcgttgctaa actcaatcgg tatcggagca gtaaagctga
     9601 gaggcgggta taaaggctac agggctgtag taaattcaat gctgccgaaa ttaaacagag
     9661 aagtgaacta tattgtcata catggctata ccgggacagg gaaaacgaaa ttgctgaaaa
     9721 tgcttgaaag cagaggttat gatattctgg attttgaaat gcacgctaac catagaggtt
     9781 ctcttctcgg agatgtgggc ttgggaaaac gaataagcca gaagcagttt gaatccctgg
     9841 tctacgacag cctgaagaaa aggaaatccg acatgatatt tgttgaggga gaaagcagca
     9901 gaatcgggaa tatagttata ccgcattata taatgcagag catgagagaa ggaaagcaca
     9961 tacttgcagt tggaagcatc gaaatgcggg ttcggaggat agttgaggaa tataccaaag
    10021 aaaaaaattc cgataaggat ataattaact ctctggaaaa gctgggaagg aatatttctg
    10081 caaggagagt cgaagagtat gcaagggata ttaggcttag gaattaccat caggtggcgg
    10141 aagatctgat gatcaggtat tatgatccga tgtatgagaa tgaaatgaaa gaatttgaat
    10201 acgaactgac tgtaaacacg gataatatcc agaaggcctg tttggatatg gaaaaatggc
    10261 tgaaaaaagc aaagccggag taaattctgc tccggccctg ttttttaata attatggttt
    10321 taccgtttca attgcctttc tggcaaattc ctgggttaca atcttatcat attcaacttt
    10381 cttatccagt tcccctgcgg tttgcattac ttcctgcagt aaatcaaggg actccggagt
    10441 tgttatggga tcggtgcacc atgcatcaat ttccttgtat ctttttacaa ctgtaaccag
    10501 gatatcatcg tcagcgtcag ggaagaatga tttgatagcc tcggctatct cttccgctgt
    10561 atggctgtgg acccagagct ggcctttata gaaagcattt gtgaacttct gtatcatttc
    10621 agggttcttg tcaataacac tttctgatgt gaaatatgcg gtataagcaa tcaaaccgct
    10681 ttcctctccc aatgaagaga ctattttgcc tgcaccttcc ttctcaagca tggatgcggt
    10741 gggctcaaac aatgccacat agtctccgac cccgcttatg aaagctcctg ctgtagctgt
    10801 aaactgcagg ttggtgatta tctcgacgtc tttacccggt ataattccat acttcttcaa
    10861 tacatattca agtgtcattt cgggcacgcc cccgggcctt ccgccgatta ttacctttcc
    10921 tcttgtgttt tcccatttga attccggttc atcgttgcgt gcgaccagga aggaaccgtc
    10981 cctttgggta agctgtgcaa atattacagc ataatcttcc tttccctgtt cgtaaacata
    11041 gatactggct tcaggtcccg caaagccgat gtcgcattgg ccggaaagca atgcggtcat
    11101 tgtcttgtcc gccccttggc ctgttgtcag ttctatttcc aggccctgtt cttcaaagaa
    11161 gcccttattg atagctacat actgcggagc ataaaagatt gagcgcacca cttcattaag
    11221 cctgacggtt gtaagctctt tcctgctgca gcctgcggaa agcatcgccg cagaagaaag
    11281 cacaataagg acaacgagta caagtgatac aagtttggca gattttctca ccaaataccc
    11341 ccctatcata gtttggatac gactgctgct attatttgca tattatgtga tatatatttt
    11401 tttgagacag tccaaggatt ttttgatgaa ctgccgcaga ggcaaagcat aaactggtat
    11461 tggaacagta taatatttat tacacttatg ggggaacata aaaatatcaa ctcggaagag
    11521 cgggtcgttc aaaaaacaac tgcatatatg aaaatgtagt acggagaggt aactatgaat
    11581 attccaaatg cattgacaat tttcagactt atactgattc cgggttttgt ctactattac
    11641 ttttcttcaa tgccgaatgg ggatagaata gcaatagtac tgtttgcggc ggcgggcgtg
    11701 acagatatac ttgacggctt tatcgcacgc aggtgcaatc ttatcacaag gctgggaaca
    11761 gtgcttgatc ccctggcgga taagctgatg ctgcttactg ttcttataag cgcaacaatg
    11821 aagaatcaga tttccttctg gataattatt gtcgtggcga taaaggagac tctgctgata
    11881 ttgggagcaa tcacgctgtt caatgatcat gacatagtgg tacctgcaaa caggttcggg
    11941 aagctctcca caattgcgtt ttatatagcg attattgctg ttgcttttga tataccatac
    12001 agcagggtaa tgcttgacgg gtttgtgata attactattc tggcattggt ggtatatgcg
    12061 aattgttata taaatataaa gcgccggcag aggatatata aaacgtgaaa cgcctgataa
    12121 aaaaataata ttgacaaatc cgcacaactc taataagata tataataatt agaaggcaag
    12181 agacaatagg cgataggtaa taggttcagc agggcttcga agcctggcat gcagcccata
    12241 ccctatagcc aattgccaat agcctcaaaa aagcaatgag gaagaggagt aggcattaac
    12301 cgccttttag agagggaaac ccacaggctg aaaggttttc cgggtacggc actgctgaag
    12361 gtagcttctg agttcctgct gtgaatagag tagcatcagg cgggtctgcc cgttacagtt
    12421 caaagagccg gtgtttgccg gaaataaggt ggtaccgcga ataaccttcg cccttaacat
    12481 gggcgggggt tttttatttt acagaacttt tcaaattcaa ctgcgaggag gatttaacat
    12541 ggacgaatca aaaaatattg caaaaactta tgacccgaaa caagtagaag agagattgta
    12601 tgagatctgg atggaaaaag gatattttac tcctgtgatc gataagaaca aagagcccta
    12661 tactatagtg atacctccgc cgaatattac cggacagctc catatgggcc atgcaatgga
    12721 caatactata caggatatac ttataaggtg gagaaggatg cagggatact gcacactctg
    12781 gctcccgggt acggatcatg caagcatagc tacagaggcc aaaatagttg agactctggc
    12841 aaaagaggga aaaacgaagt atgatttggg aagagaaaaa taccttgaga tggcttggga
    12901 ctggaagcat gaatacggcg gaaggatact taaccagctg aaaaaacttg gaagctcctg
    12961 tgactggacc cgtgaaaggt ttaccctgga tgaaggctgt tcagatgcgg ttttggaggt
    13021 ttttgtcaga ttatatgaaa aggggcttat atacaggggt gagagaataa taaactggtg
    13081 ccccaagtgc aagacttcca tatctgatat tgagactatt tatgatactg cgaaagggca
    13141 cttctggcat gtgaagtacc ccgtaaagga cagtgacgaa tatatagaaa ttgccactac
    13201 aaggccggag acaatactgg gagatactgc cgtagcggtt aatccaaatg acgacaggta
    13261 caggcatctc gtcggcaaga cattgatact gccgcttatg aacagagaaa taccggtaat
    13321 agaagatgag tatgtcgata tggagtttgg aaccggagca gttaagataa caccggccca
    13381 tgaccccaat gactttgaag taggcacgag acacaatctt ccgatgccaa ggatactgaa
    13441 tgatgacggt acaataaaca gcttaggcgg taaatataaa gggctggatc ggtatgatgc
    13501 aaggaagcag attgttaaag atttggaaga acaagagctg ttggttaaaa caaaagaaca
    13561 tgagcacaat gtgggacact gccaaaggtg cagcacaaca atagagcctt tgctttcaaa
    13621 gcagtggttc gttaagatga agccccttgc tgagcctgca atagaggttg taaggggcgg
    13681 cagggtaaaa ttcgtgccgg agaggttcac gaagatctat tataattgga tggaaaacat
    13741 tcaggattgg tgcatttcaa gacagctgtg gtgggggcac aggatacccg cttactactg
    13801 tgaggactgc ggagaggtaa ttgtatcaaa aacagcaccg ggctcctgca aaaaatgcgg
    13861 agggaagctt aagcaggatg aggataccct ggatacctgg ttcagttctg ccttgtggcc
    13921 tttttccacc cttggctggc cggaaaagac tgaggatctt gagtatttct atcctacaag
    13981 cgtactggtt accggatatg atatcatatt cttctgggtt gccagaatga ttttctcagc
    14041 aatggagaat atgcaggaag agcctttcag gcatgtcctg attcatggca taatcaggga
    14101 ttctgaaggc aggaaaatga gcaaatccct cggaaacgga atagaccctc ttgatgtaat
    14161 tgacaaatac ggtgcggatg cattaagatt caacctgata tccggcaact ctcccggaaa
    14221 tgatatgaga tttttctggg aaaaggttga ggcctacagc aacttcgcca ataagatatg
    14281 gaacgcttca agattcgttc ttatgaacct ggattatgac agggtaaaga atgcagttcc
    14341 gtcggaatac tcatcaagcc tgactgcggc ggacaagtgg ataataagca ggttcaatac
    14401 cgtagtaaaa gaagttaccg aaaacatgga aaaatttgag ttgggcattg cggcacagaa
    14461 gatttatgat ttcatgtggt cggaattctg cgactggtat attgagttgg taaagccaag
    14521 gctttacggg gatgacgagg aaacaaagct ggtggcacaa gctaccctgt gcgatatatt
    14581 aaaagggaca atgcagctgc tgcacccatt catgccgttt attacggaag agatataccg
    14641 gcacctgcca tcggaatatg aatcaattgt gatttccagg tggccggagt ataatgggca
    14701 ggcatatcag ccggaagaag agcggaggat ggaaaaggtc atggaagcaa taaggggcat
    14761 aagaaatgta agagctgaga tgaacgttgt tccttccaga aaagccaagg caattgtagt
    14821 aacagcagat aaagatgtaa aagaggctat tgaggaaggc aggctatact ttgaaaggct
    14881 ggcttctgct tcagaggttg ttatacagga ttccaaggag ggcattccgg agggggcagt
    14941 atcagtactt attaatggtg ctgagatata tttgcctctt gaagacctga tagatttcga
    15001 aaaagagctt gaaaggctta acaaggagaa ggataacctg gaaaaggaac tgcagagggt
    15061 caaaggaaag ctcagcaatc agggctttat aggaaaagcc ccccggagtg taatagaaga
    15121 ggagcggact aaagaaaaga aataccagga aatgctgaat aaaatatatg aaagaataaa
    15181 ctcactgaaa aaatgacgga gagtaaaaag cgatgaacta taatgaagtt gtggacctga
    15241 tagacacaac ggtgaaattc ggatgcaaac ccggccttga aagaactgag aagcttctgg
    15301 agctgctggg aaatccccat aaaaagctca gggtgattca tgtagcgggc accaacggca
    15361 aaggctcaac agcggcaatg cttgccagta ttctaagcag tgcaggatat agaacgggta
    15421 tgtatatatc gcctcatctg ttcaggaata ctgaaagaat gactatagac ggcattgaaa
    15481 ttacggaaga ggattttgca gaatatgcca cgcaggtatt gagcatggta aggcttatgg
    15541 gcgaaaaagg gcttgaggag cctactcagt ttgaaatgta tactgctatg gctttcctgt
    15601 attttgagcg taagaaggtt gactttgcag ttgttgaggt tgggctggga ggaaggtttg
    15661 atgctacaaa tgcagcagaa cctctgcttt ctgtgataac ctcaataggc tatgaccaca
    15721 aggatgtact cggagacact atagaaaaga tagcctatga gaaggcaggt ataataaagg
    15781 aaggttcaat ggcggtaatg tatccgcagc cgtatcctga agcatcggct gttattgaaa
    15841 cagtgtgcag ggagaaaggt gcaattttgg cagaggcgga tattgactcc ctgatactca
    15901 aggattatgg ccttagcggc cagacagttg attttaaata ccgggattat gatattcgcg
    15961 gtatgaagct gccgcttata ggggatcatc agttatataa tgccgctgtt gcattgactg
    16021 ccatagcgga gcttgataaa atggggcacc ggatagaaga agcgggtatc agaaagggga
    16081 ttgggtctgt taaatggcct tgccgtttga gcatagtaag catcgagcct ttgatacttg
    16141 ttgacggagc acacaatgag gatggaatca attctctccg taacgcgtta ttgaagtatt
    16201 tcggcaatcg aaagattatt tttgtaatcg gaatgctgag agataaagac tatgcttatg
    16261 ccataagaaa gcttatgccg atggcttatc atgtggtagc cgcggagcct ttcagtgaaa
    16321 gagcactgag cgcctctgca atggcagagg cggtgaagcc ttactgcagc agtgtaattt
    16381 cagagcctga cattaaaaaa gcaattgaaa aagcaaagaa actgtatgaa aaggactgca
    16441 tgatatgtat atgcggctct ttatatctgg caggacgtgc ctatgagtac ttaacggaaa
    16501 aaaattgaga cacggggacg ggacatgggg acgtttcgcg tgtcccgctt gcgggacagg
    16561 ggaaacgtcc ccatgtcccg tccccgtgtc ccgtctgcat gtccccgttg ttttatacat
    16621 taaacctgaa attgattata tctccgtcct gtactatgta ttcctttcct tcaagcctgt
    16681 agaggccttt ttccttaacc tttgccattg agcccagtgc ctcgatatct ttaaatttaa
    16741 ccacttcagc cctgatgaat cctctttcaa tatccgagtg gattttgccg gcggcctttt
    16801 ttgcatcggt cccttttctt atagtccagg cttttacctc gtcttcacct gccgtcagga
    16861 aagaaatcaa tcccagataa tcgtaagtgg tgtttgccaa tatatcaatt ccggagcttt
    16921 ttatgcccag ttcctccatg aacataagcc tgtcttcttc atcaagctcg ttaatttcca
    16981 tttctgcttt ggcacatacc tcaataacgg gaacatttct ttcggctgca tacttccgaa
    17041 tatcttcctt ctggggatag ctgtctgact taagggattt ttcgtcaatg tttattacca
    17101 gtatcatcgg tttttcagaa agaaagctga aggttttcag caggtctctt tcttcttcgc
    17161 taagatttat ctgatgtatc agaagcccgt tctcaaggcc ttccttgcat ttttccaata
    17221 ctttaagctc ttccaggttt tcttttgtta cctttttgcc gcttttaatc ctgtcaattc
    17281 tattgtctat aatgcccaaa tctgcgaaaa gcagttccaa atttactgtt tctatgtcac
    17341 gcatagggtc tattgaacct tcaatatgca gcacgtcagt gttttcaaag gcccttacta
    17401 tatgtaccag ggcatcgacc tttctgatat cctcaagaaa ttgattgcct actccggcac
    17461 cggtgcttgc cccgctgacc agcccgggga catcggtaaa gtctatcatt gcaaaagttg
    17521 tctttttcgg tttatatagc tttgaaagaa aatcgattct gcggtcggga atttttgcgg
    17581 agcctatatt tgaagccact tttcccgagg caaaattaga aatctccgca tgagcattgg
    17641 taagcagatt aaaaaaagtt gttttgccta caagaggcag accaaccaga ccaattttca
    17701 tggatattac ctcctataaa aattaattga ttaaagagcc aatcagttgg ctgtatatgt
    17761 tttctgagga gtttttccac ttttcgcaga tttgtttcgc ttgtttcgca gatacgacat
    17821 taagcttgag atcaataagc acaagctcgt tttcaataac cttaaggctt actatatatt
    17881 ctttatcgtc catcttttta tattctgcaa ttacctgtga ttccttcctg aggctttcct
    17941 tattttttga tatgtaatta ttgattatct gggcggtatt ttttgatatt ctttgctcaa
    18001 agattccgac ggctctgatg ccgctgtcgg tagccttaca gcattgcctt ttctggtatt
    18061 gggtatgctt tatcattccg gactcttcca actctgaaat acactgctgg aaagtgaaaa
    18121 agtctataag gttgttttct aaaatggcgt ttgtgatctg agatttggtc agaggtattc
    18181 ccaccttgct taagagatac aatatcataa gctttttatc agccaatgcc ccgctgtcag
    18241 aaatcatccg atcatctcct ttaaacagca gttctgccaa gtagacaaat taattatagc
    18301 ataattgatt ttggataagc aaaaacaaaa atcagacatg ttgcagcctg atttttgttt
    18361 atttaacatg ctgaaaggca attaattgcc ttcaagctgt tgctctgcca ttgtgataag
    18421 cttcttaacc atatttcctc cgactcttcc gcagtccttt gccgatagat ttccccaata
    18481 gtcttcggag ccttggtgta caggcaaatt taattctgaa gctatttcat atttcatatt
    18541 atcaagagcc ttatgggcat ttggaacctt cagcccttta ttgtattttc cgcttcctgc
    18601 agccattctt aaaccctcct taaaataaaa tattaatgga aaacgtgttc tttttttttc
    18661 tgtgaggcat tattaattta acctttttgt caataaagta tgttagaaca ttatgttttt
    18721 atgaataata ttatagtttg atgcatataa ttatagtttc aggaaaaata attcaaggac
    18781 aggagaatga actcctgtcc ccgtttgtgt gaagtattac gtcatttttc ttttgatggg
    18841 gatctttctt cccttatttg ttccgtttct gtgcacaccc tgctgctgta ctttttgtga
    18901 ttttgcttct ctgtggaagt gtctgtcctg cggcaatatc aattctctgg agaattcaac
    18961 catatttcct gcaatatttg taaatttgct gcgcctgttc gacatatttt ccacctccga
    19021 aataaaaaag tacaaatata ttttgcccaa aattttattt ctgaattttg cttgcggtat
    19081 atgggaaaaa ataacacccg ctttattggc gcataatgtc gaaagctgcg gggaattctg
    19141 gtataatcta tattagtata gagcagtaag ataccgaaag gagcatgaaa tgaaacaatt
    19201 gaagcacatt attgcatttt tttttataat aattattgta cttggtttta tggcatgcac
    19261 acctcagata gctgaagaac atccccggaa tccggaagaa aatttgcagg agcctgaaga
    19321 gcctgcagag gttgaagagc ctgatgaggc tgacgaacca aaagctgacc cggtagattt
    19381 aaaggcgatt aaggctaatg agaacggcca ggtaatgata ctcatgtatc atggcatcgg
    19441 agaaaaagaa gctgtatgga tgagaacagc ggaaaacttc agaaaggatc tggaggtgct
    19501 ttacaataag ggatacaggc ttataagcct taaagacctt attgaaaaca atataaaagt
    19561 tgaagcggga tacacgcctt ttgtgctcac ttttgatgat ggagcagaaa ataattttaa
    19621 tattatcgaa gaaaacggag agaaaaaaat tgaccctaat tgcgccgtag gaatacttga
    19681 ggagtttaaa aaggagcatc ccgactttgg cagcggaggc agcttcttta tatattatcc
    19741 cattcccttc agacagaagg agcttattaa ggagaaatac gagttcctta ttgcaaacgg
    19801 ctatgatatc ggcaaccatt cctataatca cgaaaacctt ggaaagctca gcattgaagg
    19861 tattcaaaaa tccttggcaa agaatgtgaa aagcacgcag gaatatctgc cggattatga
    19921 tgtatatgcc cttgcgcttc ccaacggtat agggcccaag ggggatgact acaaatatgc
    19981 agccacgggt gaatatgagg gagtaaaata taataacagg gcagtgctca aggtaggaag
    20041 caatcccgca ccgtctcccg taaatgttaa gtttgaccct atcaggctcc ccagggtgag
    20101 ggcaagcgaa atggaaacag agggcgtggg tatgtatgac tggcttcagt attttgaaaa
    20161 gaagccggaa aagcgttata taagcgacgg aaatccggat actgtagcaa taccggaaag
    20221 cgaagcggag tatgtggaca aatcgaggct gaacgggaaa gagttagtga tatataaaga
    20281 ataatacgac ggggacatgg ggacagggac atggggacgt ttcgcgtgtc ccactggaag
    20341 tgggacaggg gaaacgtccc cgtgtccctg tccccatgtc cctgccgtcc ccgtgtcctc
    20401 aaaggaaaaa ataatcaccc tgcccgtcac gcagggtgat tacgtttaac taatctcttc
    20461 gtggcaaccg cactttacgg tgtccggtta ccttataatt attatagcat aaaaggggca
    20521 aaagtaaaca tatttttgta aggagtatcc agtttatcat gggatattgc attatcacaa
    20581 aattatttta ttactgaaaa cgcatttctt caagaaataa ccttccgaat aatatatttt
    20641 caataattgt ttctttgaaa taagggtttg gcatctcgca gctgcttccc gatttttgaa
    20701 ggaaatacag ctcttcactt tctcccatga cctccgcata aagcctgccg tctttataaa
    20761 gcttgtcgcc tatcctcaga tctatatctc tgaagcttat ctgttcgaag cctcttccct
    20821 tgcaattact ttttctcttt tttttcatct gatacacctg cttaaaggat acaatttatt
    20881 gtgccacatt catattttcg acatttttat cataagaatt tataccgata tgcaactcgg
    20941 ctgataaaaa tgtatattta tcgaattata ttgacaaaaa ataacaatga atgtataata
    21001 catagcaata atagcaaaaa aaggaataaa tagaaagaaa aaacataaaa ggaaagagat
    21061 aatagaaatg gtaattatgc catgtatggg ggatatgaat gagattgctt tcagctctat
    21121 accatgaggg tatagaagaa tatatcgaat ccattccggg gattgaagta ttagacgttg
    21181 tcggaaataa aagatccttg cttgattcat taagcaagga taaatacgat ttggtgctta
    21241 tctccaaaag ccttcccggg aatgaggaag cggatttatt gatagaaatg cttgcctccg
    21301 aagagttcaa aggcctaaga acagcatatt tgtacggaga gtatgatggt tattgtgatg
    21361 atttcataaa gtttctgata aatcatggga tatatgactt ccatgtcggt gatgaaataa
    21421 cctccaagga tattgaaagg atcattttca aacctgcaag caaggagagg gcactttact
    21481 attttagaag ccattatgac aatgaaatga atatacatta cccggaacag aaaaataaaa
    21541 ggaataaacg cttctttgag ggaagattcc tgttcaattc cggatttggc tcattgttca
    21601 gcaaaaatca taagaaaaat cctaaggaga aactgattat ttctataata tcaaatcagg
    21661 ctaccggcaa atcccatacg gcttggaacc tgagccattg tttttccaaa ctgggatatg
    21721 ccacaagcct ttttaatatt gacagaggtt attcggcaaa tttgtttttt gatattgatg
    21781 aggtttatta taatctgctg gattttaccc ttcagaacga catacacaaa gatatacttg
    21841 aaaattgcta taacagaaaa aatctttgcg ttgttaccgg taaactgggt gatgagaaag
    21901 aaatcagcag tgaagacttc ctgaagctgc tttactgcat acgtacaaaa tcggatataa
    21961 caatcattga tacaaggaca ggcatatcag agctgacaag gctttcaatg cagaactcta
    22021 tttatgacat gcttgttttt gactgtgaca ttatgcattt tcatatgaat atgaaaatgc
    22081 tggaggagtt gaaggatgat tttgtacccg aaaagacaat tgccgtgatt aataatacaa
    22141 atatacggtc ttcaagccat aagttcatat ataatgaact gacaagctcg ggtataccct
    22201 ttaaagatat ggcattcgtt agcagctgcg gtttcatcag ttatgaagtg atgcacaccg
    22261 gctatacgcc gtaccaggct gcggctgaca gaaataaaag ctttttaggt gatatggaca
    22321 gtctgctgga taagctgggt atttccaacg agtaagaagg tgataagcat tggcatacag
    22381 taagaaaaac aaggtttttg cagtaaaaag aacagccctg atatcaggct gcgttgtttg
    22441 tgcagttctt atcttatcag cagtttatat gctttacggt aatgtatttt caggagaagc
    22501 ggcaggaact gtcaggaaca gcagtgaaaa cgaagcaaaa accgtggtga ttgccagggc
    22561 aaaggagaat ttggggcagg gggagctgct tgctgcaagc atggcggaat taatagagat
    22621 acctgttgaa ttggcacctg aaggagcaat taaaagcctt tcaaagttga ataacatgag
    22681 attgaaaaga ggaattgcga aaaatgagtt ttttaatgat atggacttga tgccccaaaa
    22741 tgctttttat gaaaaaggtg agaggctgat agaacataat tttgccgagg gggctgttcc
    22801 tgcctcggtt acagcgggaa gcataataga catcaagctt tttgtaaagg gcggagagga
    22861 tggcatagtc gtttcaaagg ctgcagtaat ctcgcgcagc ggtaatttgt tgtcctttta
    22921 cctggacgga atggagcagg agttcatcaa agaagcggct gcagaaggag cgctctttgc
    22981 tgttcagtat atcgatgctt cgcagcaggc aagtgaagtt acatatactc catcatatag
    23041 caaggataaa cagaactgat tggaagtgat atacatgcgc ggcgcaaaag gaaagcttat
    23101 ttgcacctgg tctccgatgc tgcatgggga agggtgctcc accttagcct gtggaattgg
    23161 aatcggactg cagtacctat cagggaagaa agtgctgatt gtcaataaga gcaattctgc
    23221 cagccacatg gaaagatacg tggagaagga tattgagatt aaatactcaa tggacaattt
    23281 aaagatattc aacaaagaaa taaagacaga gcatattttc acctatgcaa cccgtataaa
    23341 taaggattta tacatgatag ccggctccag gctgaacagg gatataatcg gggagaacag
    23401 cgaatttgac aagatctttt tagaaaagtg cctggcaggt tttgaccttg taattactga
    23461 tcttgataca ggagtcaggg atgacaacag gacgtacctg gatcaggccg acaaaatttt
    23521 agcggttgtt acaccaaatg aaattataat tgataaattg tatgatgagt atagggtacg
    23581 ggatgctctc ggttatttta cggatgataa ggcagtggta ataattaata agctctgcga
    23641 gggttgggat actgccggtg taatcggcag atacaagaac aggtactcct tatctgacat
    23701 ttttggactg aattatgacg gtgatgtctt aaatgcctgc tgcaccgaca agaatttcta
    23761 ctcctttctt atgaaggagg cgaagcgtga aaagaatata ttcataaagc agctgagtga
    23821 gatctgccgc tttcttatcg aagggctgtg catgggaaat aatgctgcgg acagaattaa
    23881 gcatgggaat atttttaaga agtttttaaa gagcagcatt tactgacggg gggagtaaag
    23941 cattggactt aggaaaatac cttattattg attcattatg cctgcatatg gaaaagtaca
    24001 tcaacagctt cattaattcg gaaccctcag atgaacagga aaaaagcata gtatcgctgc
    24061 tcagggatta caaggaaaaa tccacctccg gccttccgga ggcaagggaa gttattaagt
    24121 cacatataaa gaccggtatt cttacaggct ttgatatata tgtgtccaac gagatgggca
    24181 gactggagga tgcgattatg ttgaaagggg tatgcgttga tgaagcaaat ggacttgccg
    24241 atttgatatt acccttcaac gaaccgcaaa agcttacttc gagagagaaa ttcgagataa
    24301 ttctgcataa gaacaggaat tcatccggca acggaaggga cggagccttc aaggccattc
    24361 tcgcaaggta ttcatgctgc tgcaaggcca gggaggcagc aggttacagg agcttaaggt
    24421 atgaatatga tgaagcggat attgactata tttataataa tgaagattat gtgctttctt
    24481 tcagcgataa gatagaaatt atcacgcaaa ggctgtatga agagatattc ggactgaaac
    24541 acattgacat gctggcttat tcggatatta atgaagttgg tttttcaaat aatggaaggt
    24601 atgtctactg ctggtccgga aaaaagatat ggctgagctt tctgcagata agtgagaagg
    24661 atgcaagggt gatacaggac agggctatca gctttgaaaa gcactgcccc cagcttgatg
    24721 taagccatcc ggagatacta tgccacagag gtgacggggc tagaataaca gttacccaaa
    24781 agccgtattt ttcggcgcgc aacctatgca taaggatttt taatcaaagc tgtgccgatt
    24841 tcaatgacct gataaccgcg gataagctga ggacattgat tattgctctg gttaagaccg
    24901 gagagagcat ctgccttcag gggggattag gcacgggaaa gactactaca atgaatgtaa
    24961 tgtatgagct gctggatgat cttctgcata tcggaacaat agaggactat tttgagcagc
    25021 atattatgga aaaatatcca tacaagcgca ttgttgaagg acaggcagtc aacggcaagg
    25081 atcttctgga cgtggtcaag acaatgctgc ggatgtccgt ggatgtggcc aatatcggag
    25141 aggtcagaga cggaaatgca ttgttcgcat ttattcagtt ggtgcagtct gtctcagtcg
    25201 cagcatggtt taccacgcat ataacaaatc ccgagactac ggtgccgcgg ctgaaaaata
    25261 tgcttatcgg cacaggcagg tacttttccg agcagagcgc aataatggat attgtacatt
    25321 atattaatat gatttttcag catgaaataa tagatgggcg gataacaata acaaaggctg
    25381 tggaaattgt tcccctggtg gcggtatcaa gtgacggtga atatgatatt gagactccca
    25441 gagagcagct tgagaagctg ttttatattc aacagctgcg gagcaataca tataatatgt
    25501 tcaggttgaa taccataatg cagtcggttg atgggaaggc aaaattcgtc aactatcctt
    25561 caagaaggat ggtgaaaaaa gcaaaagggg acagacaatc atgggagtat ttaaaaaacc
    25621 ttatccgatt gattgagaag gatacgggca tggcaatacc ggaatatgca agggggtgac
    25681 ttgattaatg cggaattatt acagtaaagg cattttccat gctctgtttg cagggctgaa
    25741 caatcgcctt acccgtataa accagaaatc gcccgctcaa aacgcaagga caagcctggc
    25801 actacttacc gttttgacaa ttcttgtcat tatatatttt ctgcttgcat gcagcacttt
    25861 caagggcctt tggtatcata gagtgctgtg tggtttttcc gcctttatat ttatgtattt
    25921 gtgcaaagaa tcgtattttg agatatatga gaagcggatt aaaagggact tgcccaatac
    25981 cttaaagaag cttactcatt actacaatca ttacagggga aatatcattc cggcattgga
    26041 agatactgtt tccagatgcc ccaggagcaa tagaatctat atgatagaaa tcagggaagc
    26101 actgatgaag ccggactatc aagtacagat agaggaactg gagggcaaaa tgcctatgat
    26161 ttggctgaaa atgctgtgcc ggcttattct ttccgcaaag gagaatggag gtatgtcctc
    26221 ggagggagac accgttgcaa ataatctgaa gcgaatgacc aatataatta catttcttaa
    26281 tattgaacaa gggtataatg atgccgagct tctgggaatg cagatttttg tgttttttac
    26341 tcctttttta gtgataccat tgacaaaatg gtacaatatc agtttgcttt caggccttaa
    26401 cattggggac atatatggaa gcatacaagc ccaggggctt actgcaatta tgatgtttat
    26461 cagcggggtc ggaacaatat tcattcattg gatgagaaag cttcaaaact gattaagggg
    26521 ttgagcaaca ttcagtattt catattgatt caagctattg caattgcttt gctgatagct
    26581 gcagcttcta tatttgtcca tcagtatttc aggtatagga aaatcaaaag gttatatagg
    26641 gctgattatg ccaggaaaag ctgcttgaag agcatttcca ggttcctgat ttttatgccg
    26701 ggatataaaa gcagaagata caaaaataca gagggattga taataaactc aggattgagg
    26761 gtttcagtcg aggaatttta tttggcaaag acattgcttt ttgtcctcgg ctttgtgttt
    26821 tttatcagtg tgcaaactac aaatactttt gccttctacg accatgttat cagcgatttg
    26881 aacatgggaa atacattgat agatgaagct gcaccaaatg attatacatt gcagcttgaa
    26941 aaagaggttt tcattcacat agacagcagc ctgcccaagg ggaaagcggc cctgaaggaa
    27001 cttaaggatg aaaacaattc ccaggtttac atcggatata ttgaggctca gataagcagt
    27061 aaatgggctg gactggaaga gtatgcaggg gaaatggctg agaggatgta taagaaacta
    27121 ataaaaatca gaaggataga aacagactac ctgatttatt taacttcatt tatactggca
    27181 ataatactgt attttatccc aaacataatc ggacatttaa aactaatact tatagaggac
    27241 aaaagggact gggaaatact gaactatatt tatgtatttt caatgtttgg gcgaatgccg
    27301 ccctttaata taaaaaatgt gctttccaat attttggtga tttcagatat ctataaacca
    27361 ataataaatg aagccttgaa taatttaaac agcggaaaag gggaagaggc ctttgacaac
    27421 ctgctgcaaa aagtggaaaa ccaggagctt tatgaactcc ttgagtccat gcgcctttct
    27481 atgagcacag ggcttctcaa catagtggac aacattgatg aaatggcggt aaaccagctt
    27541 aaatggctgg aaataaagag tttaaaaaga agaaagtcga agcaggtaat cgcaatggtt
    27601 ccggtagtac tggtaatgct tgcggcagcc gtatattttt cctacagcct ttcgacactt
    27661 tcaaatccaa tgaactattt aaaataaaat ggggggagta tgaatcatgg aaaaattctg
    27721 ggcggtaata ttgatatttg ttgtggttgc ggcagcgggg ctgatgtatg tcaatcaggt
    27781 gtatattcct cagaaagaga cgcagatggc aatggcggat atggcgaatc agactctatc
    27841 tgcagttaaa gtggagatag gggatgatag tgttcctcaa gaggttagtg ggtctgaaat
    27901 aaaagatata atagaggatt actcagatga tgatataaag gtttacatta atggaataat
    27961 ttatgatggt actaatgtaa ttgaacattc cagccagtac tatatgagag taataaagaa
    28021 tgatggtgag ataactgaga tcagatttac gataattgtg tagttaaatg cgacggggac
    28081 acggggacac ggggacgttt cgcgtgtccc actgaaagcg ggacagggga aacgtccccg
    28141 tgtccccgcg tccccatatc cccgccgcat tattcttcag atgtgaaatt ataactgcaa
    28201 ggagattatg caaatggaaa aattctgggc ttcaatattg gttatcccga tttttacatg
    28261 gtactccttc catgccgtcc tggacaggga tttcaggata aagcagcagg cagtggagaa
    28321 tatagtatgt caatatacac agatagctgc aaaaaaaggt gccttgtata attctgtcta
    28381 taatgagatg aaagaaagat tatcaaggtt cggtacattt gatatcagtt tgacggcaga
    28441 gaggttcacc ggcagcagcg atacgccgga actgataaaa ggttcatata taataggaag
    28501 ggatttgcgc tcggaaggtt ttgacataat aaacatatat gctgagagca ttaaccgtca
    28561 tccccttgga aggttgtatg aactcacacc ttttggaaca aagtcaggtg cggaagcaga
    28621 tatcaggtat ttcggcaaag catcggtata tattcaatag tataggaagt ggctcgatga
    28681 agtcgttttt ttggagtttg atttttatat tctgcacatg gagcatgttc ttcataggga
    28741 agggagtaga caacatagac attatggctg agaggtacaa gagggcactg gatgccggag
    28801 caaatgcagc ttccggatac agggcctatt attctgagaa aatgctttat aatcatggta
    28861 caggattcgg cattggcctt gaggacagca ataatttgcc ggtagatagg gaagaggctg
    28921 ttaaatggtt ttaccggttg tttttcagga accttggtat agaggacacc aataagcaga
    28981 atgagtataa gaggtatatg cctatgaaag ccataatcct ttttgacagg cttatgatag
    29041 ctgatgcgga tgacaactgg catacatatg atcctgcagg ggaaaaagaa tacatgatcc
    29101 aatacagagg aaaggattat aagttcacat tgtctgatca ggtctatgac attgaaaaca
    29161 gctcgtggat tatggcagat gatatagggc tggaggcaaa agaaagaaaa gagctggtca
    29221 cgaattacat aatcaatgag cttaacagct ttttggataa cagaggcaat aaggaaagcg
    29281 gcagttatta caagatagta ttctctctgg acgatgcttc agacagtaag ctcagcggaa
    29341 taaacgggat taactttttt atactatgcg aaggaatacc cataccatca cttaatccct
    29401 ttaaacagga aaagtttttt gcttacagcg taggcggaag cgagataaaa aagaagcagt
    29461 tgtgatgata cagatgttaa cgactccccc acctgaaatg ccaacgcatt ttttaggtgg
    29521 ggggcctttt tgcactatac tatgtattaa tatgcagaaa aatgtcgaat tattactata
    29581 tttgtattac tgttattatt ggaaatttca aacttttgta agataatata tatgctggca
    29641 taaatccggg tgtcatggga attattattg actgtaagat taataaataa attttcttat
    29701 gggggagctg aaaatcttga gtgttctgcc ttacattact gccgtagaca atagagtgga
    29761 ggatgtaaat aaaccttttc ttgatcttac gggatatacg gaaagtagta tccggggaag
    29821 aaatgtagag gagatatggc agcagcttct aagaataagt atggacttgg acagtgtaaa
    29881 gtgcttgaag gaatgtagct gctatatgtt tgacatcctg cgcagggcaa aggaagtgac
    29941 tataagctat actgcggact ttgagctgaa acaatgtatt tatactttca aacggaaagt
    30001 gaattccatg cttgaggaca aatttccatt tcttgagcat gcaaattccg ccaatgatac
    30061 gggagtagcc ctttacagtg tgccggaatt catactcctg aaagggaatg agagtcataa
    30121 caggttttta agcaggctgc tcggcggtgc ggaagacatt atcggaaagc ggattgagga
    30181 gattctgccc gattggaagg gaagcgatgc ggaaaatata ttcagcaatg ttgtaaagac
    30241 aggcaaacca atattctatg atgaacatat gagggaacat ccgtcaacag gcattgtctg
    30301 tacgagatat actcttaccc cgatttttga gggagaagag ttaagatacc ttgtgcttat
    30361 tcacaatgac gttactaaaa atctgacaat agagagagaa aaagattatt ttgaaaagca
    30421 gagggattac ctgtacaggc tgttcaatgc catgaatctg ccaatcatat cgttttcata
    30481 ccccgaatat aggattaagg aatttaacaa taaagcattg gttgagttga gcgaactgac
    30541 aggcatggat aatttgacta ttgacccgag tatttcttgc aaaggcctgg ctgaaatgat
    30601 accacttttt gatattagca gtgcaaagat attaattgaa cagatgaagc aaaccaaatc
    30661 tcctgtgtgc cataaaaaaa tggaactatg taaaaaaggc cgcaaggtat attatgatgt
    30721 tacttttcat ccttttttga acataatgga ggaaatttca gagatattga tagttgctgc
    30781 ggatgttacc gacgaagtgg aaaaagcgaa gcatatagag aatatattga agcttaagga
    30841 tgaattctta tattttatta ctcatgagtt taagactccg ttatcggtta taaatgcggc
    30901 agtacagacc ttggagcatt gttactcaat gcagattcct gacaaagcca ggtttttaat
    30961 tggaaaggtt aaacaaaatt catacaggca gcttagactt gtaaacaatc tgctggagat
    31021 tgcacgtata aattcgggct atatcaagct cagacaaagg aatgtggata tagtttttct
    31081 gacaagagct attactgagt ctgtggcaat ttatgcccag caaaaaggca tcgaattggt
    31141 ttttgttaca aaattcccgc aaagagtaat tgggctggat gatgaaaaac ttgagcgcgt
    31201 tcttcttaac ctgctttcca atgcaattaa atttacaccc gcaggcggaa aggtagtggt
    31261 ggaactgtcc tcaaagctgc ataagcacaa aagggttatc tgtatcaagg taaaggatca
    31321 gggtgtcgga atccctaagg aaaaacagtc tgttatattt gatcgtttcg gacaagtaga
    31381 caatttgctt accgtccagg ctgagggcag cgggataggg ctgtatcttg tcaagcttat
    31441 ggtggattcc attgggggag aaataacttt ggagagtgaa tggggtaagg gcagtgtgtt
    31501 tactttaatg ataccctcaa aaaaatcaaa ggagaatgcc aaagagcaga aattcaagct
    31561 cagatccgac aatcggctta tacaaaccac agctattgag ttttcagata tatacttata
    31621 aggagtcaat tcgaatcaat tgcatcggaa ggcagcgtta tattttcaac aatgtattgg
    31681 aatatgccca ttggagtttc caccttaaca tcatccttga ctttcttcag aaggaatgcc
    31741 ctgcccatag gcgacagata tgacgcacta tcgatattaa gatctgcttc gcccataaaa
    31801 ggagatacaa tctgcatttc ctcgattttg tttgtataac aatccttcag aataacctga
    31861 cttccgatta ttgcaaaagg gcaagtatcg gatgcattgt cgtccacaag aacctcattg
    31921 tatataatgt tttcaacacc cttaatataa gaatccagga gttcctgaaa atcattcctt
    31981 tcatttgtcc acacaggata atatttatca agtacatgaa gcttttcttc ttctattcta
    32041 accaggtgat taacaagcat ttcatatatt ttttttgata taatagattt tgacaccatt
    32101 aatacctcct aaatattaca ttaaatggta aataaaaata taatacaaca aggcaccttg
    32161 cagcaaatct gcaaaatgcc tgtaaataca gtttaacata aatgtaaatc gatgtaaata
    32221 gtggattgca tttccattta attgaaatgc tggatttttc gcacaaaata ttcttaattg
    32281 ctcaaaatac atcttgacag ctattaagat atagattatt attatatcat accaaaatgg
    32341 taggaattaa gaaaaggctt tgaacggaaa gtgtaggcac acaaatattt cttcagagaa
    32401 aggctgcatc ggattgctcc gattgctgaa agcagcctta gggattggtg ctgaaagtgc
    32461 gtccgggagc ttttgaccga acaagctaaa acaaaggttt aagtaggata taacgggagc
    32521 acccgttaca gtgcagggat atgttagtat ccttaaaaag taataaaaga gtggaaccgc
    32581 actttatgcc tctttataca tatgtatgta tgtgtaaagg ggcttttgtt tccataaacc
    32641 aaaaatctat agtaaaaggg tggtaataat gtttggacga ctgaaggaag acataaggac
    32701 aatatttgaa agagatcctg ctgcaaaaag ttatcttgag gttatattgt gttatccggg
    32761 cctgcatgcg gttatttgcc acagaattgc tcacggcttg tatcgcagga ggcggtatgt
    32821 cgcagcaagg ctgatttcgc aaataagcag attctttacc ggaatagaaa tacaccccgg
    32881 tgctgcgata ggaagaaggt tttttatcga tcacggttca ggaatagtta taggcgagac
    32941 aactgaaata ggtgacgatg tgaccttgta tcagggggtt actttgggag gcacgggaaa
    33001 agataaggga aagaggcacc ctaccatagg gaataatgta acaatcggct caggagctaa
    33061 aattcttgga ccaatatcaa taggacataa tgcaaaaatt ggagccggtg cagtagtggt
    33121 gagatgcata cctccgaaca gcacggctgt cggtgtacct gctcatgtgg taggaaggga
    33181 aggaatagac gagacctatg ttgatttgaa ccatacggat attcctgatc cccttgaaga
    33241 tgaaatcagg gaactggaga agagaatcag ggaattggaa cgaaaacttt tttctattga
    33301 ttaaagctca gaataatatt aacataacat tagcagtaat tattatattc agaggtgata
    33361 gaaataaaag aagtatttaa aacagttatg aaagacggat tagcgtatct tacaataccc
    33421 gcctttgaaa aaaccggcat ggtaaagcac tgcttcacta caaggatagg cggtgtaagc
    33481 cggggcatat acaattcact taatacctcc gatgataaag aggatcctgt ggaaaacgtt
    33541 cataggaacc tgaaattggt gtgcggagct ataggtattg attatagcaa tctggtgttc
    33601 tctgatcaga cccacgagga taatataaga gttgtttcaa ttgcagattt aggaaaaggt
    33661 atatccgtac cgaatgatat aaagaataca gacgggttaa tgacaaatat acccgggata
    33721 cccttaataa ccttttatgc tgactgtgta ccgcttttct ttctcgataa agaaaataaa
    33781 gcaatcgcag ttgtgcattc gggctggaag ggtacggttc aaaaaatagg agtaaagaca
    33841 ataaggcaga tgacagaggt atatggaaca aaaccggagg attgcctggc ggggataggg
    33901 ccttcaatag gcccggagtg ttttgaggtt ggacaggagg ttgcggatca gtttactgaa
    33961 agctttggca cccggcctca tatcataaag ccgttaggga acgggaaata catggttgac
    34021 ctgtgggcag ccaataaact gatgcttatg gaaatgggag tgccggagaa taatattaca
    34081 atcagcggat tatgcacaaa atgcagggaa gatttgtttt tttcatatag gagggacaaa
    34141 ggcagaacag gaagcatgtc ggcaataatg gaactgaagg aggaagatac ctgatgaacc
    34201 ggaagatact tattatagag gatgaagcaa atattcagga acttcttagg tataacctgg
    34261 aaaagaacgg gtatattgtt actatttcag ataatggcat tgacggactt cagaaagctc
    34321 ttgaccaagt gcctgacctg atactgcttg accttatgct gcccggactt gacggtttgg
    34381 aggtatgcaa gaggttaaga atgcaaaaag caacaagaag gattcctata ttcatgctaa
    34441 cagcaaagag tgaagagctg gacaagatat tgggcctgga gctgggagct gatgactata
    34501 ttacaaagcc tttcagcata aaggaactca ttgcgagaat aagagctgca atgagacggt
    34561 tggatgatga aagcagcgct gtgtctgata aagcagagaa aaataaaata ctcaaagcaa
    34621 gggatataga aattgacagc gagaaatatg aagtaaggaa aaacaatgaa aaaattacac
    34681 tgacccttaa agaattcgag cttttgaaaa tgcttgtaga gaataagggt aaggttttga
    34741 caagggattt tttgttggat aaaatatggg gatatgaata tatgggtgat acaagaactg
    34801 ttgatgttca tatcaggcat gtaaggcaga agcttaatga tgatgaaggc agcggccgga
    34861 tgattgagac tataaggggt gtaggctata gattcataag tgaaggtgaa taataatgca
    34921 gcgaaaaatt ttttttaata ttgtcgttgt acttcttctg ggagtgatca tttcaggttt
    34981 attgtctgca agaatagtga aaaaaaacct tctcagtgat attgagaaaa gtcttacaac
    35041 agaagccgct cttgtaaggg agcttctggg ggaaagctta ataaacaaca gcaacgatat
    35101 aggcatttat gtcggtaaga taaaacagac tgccaactcg cgaataacaa tagtagatgt
    35161 tatgggcaat gtaattattg ataccgataa ggaatactct gaaatggaaa atcatgccgg
    35221 aagacctgag attgcagcag ctttaaaggg cgaggcagaa agttctataa ggtatagcag
    35281 tactcttaga gtggatttta tatatgtggc acagcctatt ttgaaaggaa gcagcataat
    35341 tggagccgtg aggctgtcga aaccgctcta tgaagtaaaa aatatgctta atagtttgta
    35401 tatcaatgtc ttcattgcag tactaatagg tatttttgta gcttcagtgc ttggatataa
    35461 aatggcgatg aatataacca agccgattaa ggagattacg tataccgcat caagaattgc
    35521 aaaagggcag ttagacagac ggattaacat tacaagccgg aatgaaatag gtatattggc
    35581 agattccata aatgacatgg catcaaagct gagggacact ataaccagcc tgcaggataa
    35641 gaatataaag cttgaatcta taatgtccag tatgataaat ggaataatag caatagacaa
    35701 ttcagaaaaa atactattta taaaccatgt ggcagagagc ctcctgggga ttaccggcgg
    35761 agaaatcaca ggaaagcatc tccttcaggt tgtaaggaac aattccattg ataattactt
    35821 taaaaaaatg ttaaaggata aagagttttt taatacagag gtgaatctta atgactccaa
    35881 tgaaaagata ttgaagtttc atgccaattc tataaagcag aatggtgaaa atggtattga
    35941 aggaattatt ataaccatac aggacgtaac tgagcttaga aaactggaaa gagtcaggac
    36001 ggaatttatt gccaatgttt ctcatgaact caaaacccct ttaacatcca taaaggggtt
    36061 tacagagaca cttaaatcat ttgattttga tgataaacag gatgcaataa ggtttttaaa
    36121 tataattgag gatgaagcag acaggcttta caggcttatt aacgatatac tgtccttatc
    36181 cgagttggaa cagaaaaaaa caaagactgt taaagaaaaa atagatatag aaaaagctgt
    36241 agaagaagtg ctgtcagtac tgaaaagcca gagtgacaaa aagaatatag aactgtcagc
    36301 gaagattcag gaggggcttg aaagtatttc aggcgatggg gacaagttca agcagatgct
    36361 gataaacctg atagacaatg cagtaaaata tactcctgaa aatgggaaag tgagtataga
    36421 ggcatataag caatcacata atgctgattt agacaagctg attataaaag taaaagataa
    36481 tggtattgga atacccaagc agcatatacc aaggcttttt gaaagattct acagggtgga
    36541 caaagcaaga tcaagaacaa taggaggcac cggccttggc cttgcaatag tgaagcatat
    36601 agtaatgctt ttcaatggtg agatagaagt aaccagtgaa gtgggaaaag gcactgagtt
    36661 cagaataata ctgcctatga aatgataaaa actgcaattt tgcagttttt ttttacatgg
    36721 aattaacaat tgcttaatat ttcttttacc atacctttat atattgttaa cagtactgtt
    36781 gtatattcta attgtgataa gaaaaaaata atacgatgaa ggttaaggag gatttattag
    36841 gatgagaatt aagaaaacac ttgcattcat ttgcgcagca gtattgctta taggtgcttt
    36901 aacaggatgt gcgccggcag aaacaaatga accggcagct aatggtttgg gtgaaagtat
    36961 aactataaat ggatcaacat cggtgcagcc gctggctgag gaacttgcaa aagcatttaa
    37021 agaaaaaaat cccgatgcaa cgatagacat acagggcggc ggctcaggtg taggaataaa
    37081 atcggctgct gacggagttg cagatatcgg aatgtcttcg agggacctca agcccgaaga
    37141 aaaatcatta aaggaattta aaatagctgt tgatggtata gcagtaatag taaatcccac
    37201 aaatgaaata caaggccttt caatggagca gatactcaag atatacaccg gagaaatcac
    37261 agattggaag gaagtaggag ggaaagaagg gaaaataacg gttgtaacca gagaagaagg
    37321 ttccggtaca agaggagcat ttatagagct aaccaaagtt gaaagcaagt cagaaggcga
    37381 caggactgtt gcttcagcaa taacacaggg ttccacaggt gcggttatta caacggtagc
    37441 gggggatccc aatgccatag gctatgcttc cttcggatcg gcaaaagaaa atacggacat
    37501 aaagctgata agtgtggacg gaaagacatg tactgaagaa aacatatatg ccggagaata
    37561 caagatatcg agaccttttc tgatgctcac acaggcagaa ccgacagggc ttgccaaagc
    37621 attcctggat ttcatactaa gcgaagaagg acaaacagtt gtaagtgggc ataattatct
    37681 taaggctgtg aaataagacg gaaaagggga gcaaatatga aggcttatga acgggtgata
    37741 caactggtac tttttatcag cgccataata gccgtgtttg gagttatact gatttcatac
    37801 tttataatca gcgagggata tcctgtgatt cgcaaggtcg gattattgga tttcctttca
    37861 ggtaatagat gggcgccaac acaaggagtc tatgggatat atcctatgat tgtaggatca
    37921 ttgcttgtta cagcaggggc attggtgctg ggagtgccga taggaatagg ctgtgcaata
    37981 ttcctggcag agctggcgaa gcctagaata gcaggaatcc tcaggcccgg aattgaattg
    38041 ctggcgggaa taccttcagt tatttatgga ttgtatggac tggcagtaat tgttcctttc
    38101 atcagaaaag tgttcaataa tcagggcttc agcgttctgg cagggagcat agtattggca
    38161 gtaatgattc tgcccacaat aataaatgtt tcggaaagtg ccataagatc agtccccagg
    38221 gattataagg aagcatcctt gtctttgggt tcctcccact ggcagtccat aaaaaatgta
    38281 atagttcctg ctgcacgttc agggattact gccgcagtca tacttgggat gggcagagct
    38341 attggagaaa ctatggcagt aataatgata gtcggcaaca gtccgttagt tcctgaaagc
    38401 atattgtcac cggtcaggac acttacaggc aatataggca ttgaaatggg atatgcagga
    38461 ggagaacatc agaaggcact ttttgcaacc ggtattgtac tctttgtttt cataatgctg
    38521 cttaatatag ctgcacacct tatcccaaag aaggcaggtg aatgaattga cggaatcgag
    38581 cattacttca ggaagtgtaa ataaaatcag aaagaatgaa aagacagcct ttatatttct
    38641 ttgggcagct gcagctataa cggttttgac tctgatagct attgtgggga gcgtgctgat
    38701 aaacggaata ggtcatttga gcttggattt cctgactaag gagcctgaaa acatgggcaa
    38761 ggacggaggc atattttcaa taataataac aaccatatat ctggcattat tttctctgtt
    38821 aattgcagca cctataggca taagcgcagc aatattcctt actgaatatg ccaaagaggg
    38881 ccctgtcata aggctgataa ggtttggaac cgagacactg gcaggaattc cttccataat
    38941 ttttggcctt tttgggtatg ttttctttgt tgtgttcctg aaattcaggt attccatggt
    39001 atccggaggg cttacactta catttatgat acttcctaca ataataagaa cagcagaaga
    39061 gtctataaaa atggttccaa tgtcttatag ggaaggaagt cttgcattgg gagcaacaaa
    39121 atggcagaca atatataagg tggttctgcc tgcatcaatt cccgggattc ttaccggagt
    39181 catattgggc ataggcaggg tggtaggaga gacagcagca gtgatataca cggcaggaag
    39241 ctcacttgga ctgcccaatt caatatggag gcccgggagg actatggcgg tacaccttta
    39301 tatattggca tccgaaggat tatcaaagag taatatgtat gcaactgcaa ctgtattaat
    39361 aataactgtg cttataataa actttacagc caacaggctg ataaagcaat ttataaggtc
    39421 atagcagagg gggatgaaaa tggcagaaaa caagattgag gtcagcaaac tgaacttgta
    39481 ctatacagat ttccatgctc tgaaaaatgt tgatataaat attgcaaaaa ataccgtcac
    39541 ggccttcata ggtccctcgg gctgcggtaa atccacattt ttgaggaccc ttaacaggat
    39601 gaatgatctt atagacggtg taagaatcag tggaaaaatt tctcttgatg gtgctgatat
    39661 tcaaaaatat gatgtgatag aattaagaaa aagagtaggt atggtatttc agaagcccaa
    39721 tccttttccc atgtcagttt atgataatgt tgcatacggg cccagggttc acgggcttag
    39781 agacaggaga aagctggatg acatagttga aaagagtctt aaaagtgctg caatatggga
    39841 tgaagtaaaa gacaggctaa aaaagtcagc attggggcta tcagggggac agcagcaaag
    39901 gctgtgtatt gccagggttc ttgcggtaga acctgaggta cttcttatgg atgagcctac
    39961 ctcagcactt gatccgggtt caacccttaa gattgaggat ttgatggagc agcttaaaaa
    40021 ggactataca gtagtaatcg taacacacaa catgcagcag gcgggccgta tttcagacta
    40081 tactgctttt ttcctcaatg gagagatagt ggaatgtgat ttgacggaga acgtgttcta
    40141 taaaccgaga gataaaagaa cagaggatta tataacagga agattcggat aaacagtgag
    40201 taattgaaat ggaggtgtga ggatgacacg taattatttt gaccaggaat tgcaggagct
    40261 gcaggtatcg ctcctgaaaa tgagcagtat ggttgaggag atacttggaa actccgtaaa
    40321 ggctcttaag gctcaggatg cagagttggc gcagaaggtc attgatgatg atgatgaaat
    40381 agataccatg gagtatgtag tggaggacaa gtgtatcaag ctcatagcac tgcaatcccc
    40441 ccttgccagg gatttaaggg taattgccac agcacttaag ataattacag accttgagag
    40501 aatagcagat tatgcagtgg atattgcaaa aataacaatt aagcttcagc atgaaaagta
    40561 tataaaagag cttatagata tacccagaat ggcagaaatt gcagtaagaa tggtaaaagg
    40621 ggctatagat gcatatgtac atctggatac ttcccaagcc catgaaattg caaagcttga
    40681 tgatgaggta gacgggctgt ataaacaaat tttcagagag ctgttgttta ttatgatgga
    40741 ggatcccagg accgttcacc aagctactta tttccttctt ataagcaggt atcttgaaag
    40801 aatatgcgat catgtgacaa atatatgtga gcgcataatt catacagtaa caagtgaaca
    40861 tgtgaattta aatgagtaaa aggatatttc ggcaaagtat tcttttattg ttatacccct
    40921 atagtataat atgaagctga tatgatataa tactatgtat aattattggc gaatgatggg
    40981 tttgtatgta agggaggaaa acaatttggc gaatccgggc gtagtaataa tggaagtacg
    41041 tccttcaagc atagcagagg aattgggcat aaaaaaaggt gacaggctca taagcataaa
    41101 cggcgcaaga ataaaagaca ttcttgaata taagtatctc actgcagatg agtttcttga
    41161 ggtggaaata gagcacggca atggcgaaat atggatttat gaggtagaaa aagagtatga
    41221 tgaggatata ggaataatct ttgagggaat tattgacaga ccaaagtctt gtcataacaa
    41281 gtgtattttt tgttttattg accaactgcc ctccgggatg agagaaacct tatatttcaa
    41341 ggatgatgat acaaggttgt ctttcctgca gggtaatttt gtgactctta ccaaccttag
    41401 tgaaagagag atagacagga taatcaagta tcacataagc cctataaacg tatcggtgca
    41461 tacaactgat cccggactta ggagaaaaat gctgaacaac aagaatgcag gaaagctgat
    41521 ggattatctc gaaaggctta aggaagggaa aatagaggtt aaggcacaaa tagtgctttg
    41581 cccgggagtg aatgacggag aaaatctctt aaagacttta gaggatcttg ccggactgca
    41641 tccccaggtt agctgtgttg ctgttgtacc tgtaggtata accaaataca ggcaaggcct
    41701 atatgagctt gcggaatatg atgggatatc agccgcttcg gtaataagga tggtcagaaa
    41761 agcgcaggat aagtacctgt ccgaactgaa tacaagattt gtatttctgt ctgatgaatt
    41821 tttcctcata tcaggggagg aactgcccgg gtttgaggag tatgaagaat ttacgcagct
    41881 tgaaaacgga gtaggcctta ttgcactttt taacagagag gttgaagagt ccctgaaaaa
    41941 gcttggaaga tataataaga aaacgaggct gatttctata ataacaggag aatacgccgc
    42001 accaaatctt aaaatggcgg catcaaaaat tgaaagctgt atagaaggcc tcaaaatacg
    42061 tattttccca ataaacaacg actttttcgg gcccggtgtg aaggtagccg gcctgcttac
    42121 aggaaaggac attatggatc agcttaagga taagaaagtc ggaagggata tattcatacc
    42181 tgattgcatg ctgaaaaagg atgagaaaat ttttcttgat gatgttactg ttgaagagat
    42241 ggaaaaagag cttggagcta aaatcacaat atgcagggaa gacggcagtg accttgttaa
    42301 gaaaataata tcggtttaat ggattagtgt caggaggtga ggcatattgg caaagtcaat
    42361 tgttgcaata gtaggacgtc ccaatgtcgg aaagtcaact ctttttaaca aattggcagg
    42421 taagaggatt tccatagttg aagatcatcc cggtgtaacc agggacagga tttatacaga
    42481 ggtccaatgg caggacaggg catttacatt aattgataca ggcgggattg agccgcatag
    42541 tgaggatttg atgctgaagc agatgaagca gcaggctgaa gttgctatag aaactgctga
    42601 ggttatcgta ttccttgtag acggacagga aggtataacc ccttccgacc gggaggtggc
    42661 caatttacta cgaaggaccc gtaagaagat aatactggca gttaataaga tagatgcacc
    42721 gaagtataag gacaatatat acgaattcta taatctggga ttgggggaac caatggggat
    42781 ttcttcgggg caggctttgg gattggggga tctgctggat gaaataatca ataacctttt
    42841 accggaggaa gaagaggtat atgatgaata cacaataaag gttgctgttg tggggaagcc
    42901 caatgtcggc aagtcttccc ttgtaaataa gatactcgga gaacaaaggg ttatagtcag
    42961 tgatgttccg ggcaccacaa gggatgccat tgatactccc tttaccgtag gagaagacaa
    43021 gtatgtattt attgatactg caggaatgcg aaaaagaggc aaggtgtttg aatcaataga
    43081 gagatacagc gttatcaggt ctctaactgc cattgaaagg gcagatgtgt gccttatgtt
    43141 aatagacgga aaagagggcg taagcgagca ggacagtaag attgcaggtt atattcacga
    43201 tcagggaaag gctgctgtaa taatagtaaa caagtgggat atagtggaaa aggatgataa
    43261 gaccatagat aaatataaag cggatataag aaatgaactt tcctttatgg agtatgcacc
    43321 cattctattt gtctctgctt tgacagggca gagggtgaac aaggttctgg agctggttaa
    43381 ccatgtatcc aaccagcatg ccatgagaat ttcaacaggt atgctgaatg atgtgataaa
    43441 cgaagctgtg ctgactaatc agcctactgt ctcaggagga agaaggttta agatttacta
    43501 tgccacacag gtttcaataa aaccgccggc ctttgcactt tttgtaaatg agccctccct
    43561 gatgcatttt tcatatcaaa ggtatctgga gaatcaaatc agaaaagcct tcagctttga
    43621 agggactccg gtgaggtttt tattgaggaa gaaggagtag aaaaaagggg ggtgcttttt
    43681 atagatgaaa tatgtatttg tgagtataat tcaggatccg caaatgtctt tagggtattg
    43741 ggcgcaaagg gcggaaaagg cattgcaaca acaattggac tgatgctgcc catgatcctt
    43801 taatggtagt cctcatattt attgttggtg ttatagttat tatcactacc agatatgttt
    43861 ctccggcatc aatagtggga gtgacaatat atcccatagc aatgataatg acacggaatc
    43921 caaatttgga caaaagaaga taacagagca ggagggtaaa ggatgagcaa caatatttca
    43981 ataattggtg ccggcggctg ggggacagcc gtatcggtgc tgctttcagg aaaagggttt
    44041 aatgttaagc tttgggcaag ggacaatgaa ttgattgaaa aaatgaggga cacaagagaa
    44101 aatcctgctt atctgccggg tgtgacatta aaggaaaata ttcttgtatc ctctgacatt
    44161 gaatactgct gcagcgggag tgaaataatc attatcgctg ctccttccca tgcaatgagg
    44221 gatacgtgca aaaaaataaa ggaatttgca tcaaagaagc agattattgt cagcctgtca
    44281 aagggcattg aaaacattac actgcaaaga atgtccgaga ttataaatga aatcatgact
    44341 gaaaacgaag tggctgtagt ttcgggcccc agccatgcgg aagaagtggc cagaggcatt
    44401 cctaccgccg tagttgcatc atctccaaaa agagcagtgg ctgagtatat acaggatata
    44461 tttatgagtc ctctattcag agtatataca aatccggata ttatcggggt tgaactgggc
    44521 ggggcattaa aaaacataat tgccctgggt gcggggatta ttgacgggct tgaattgggt
    44581 gacaatacaa aagcagcggt tatgaccagg ggcatagttg agatggcaag gctgggagag
    44641 tccctggggg cttgcaggag cactttcaac ggcttgtccg gtataggaga cctgattgtt
    44701 acttgtacaa gcatgcacag caggaacaga agggcaggaa tagccatcgg acagggcaaa
    44761 tctgtggaag aagtgattag aggtacaaga atggtggtgg agggtatcag gaccaccaaa
    44821 tccgcatatc ttctggcaca gagacagaag gtggagatgc cgattaccca ggaaatatat
    44881 aacctgcttt ttaataaaac ggatataagg aattcggtaa tgaacctgac catgagatcc
    44941 aagacccatg agatggaaga agtagctgaa aacaaagtat ttgaatggta ggtatatccc
    45001 tttgaagctc gaagattgct tagcaatctt ctttttttgt aataataatt gcacatgcac
    45061 atatatattt agagaaggac tatgcaatta tttgaacatt attaaggagg gaagagtgtg
    45121 gaaggatacg atgtttatca agatatatca gagagaaccg aaggctgcat ttacatagga
    45181 gttgtgggac cggtcagaac aggcaaatca acatttataa agagatttat ggatttgctt
    45241 gtgcttccta atatggacaa tgagttcaaa agagaaagaa cccgggatga gcttccccag
    45301 agtgccaacg gcaagacaat tatgacaacc gaacctaaat ttgtgcctaa tgaacctatt
    45361 gagctgactt taagagagaa tgcgaaattt aaagtcaggc tggtagattg tgtaggatat
    45421 cttgtggaag gtgcaatggg ccatttggag gataacagcc caaggatggt taatactcca
    45481 tggtttgaag accgcatacc ctttgaagag gcggcggaga tcggaacaag aaaggttatt
    45541 gcggatcatt ccacaatagg ccttgttgtg actaccgacg gatccataac tgatcttcct
    45601 agaaggagtt atatagaagc ggaggaaaga gtcattaaag aactgaagga gcttgaaaaa
    45661 ccatttatag tggctctgaa ttccgcaagg ccgaaggcac aggagacgat tgaactcaga
    45721 gaagctcttg agacgaaata taatactccg gtcatgataa tagatgcctt gaaaatggaa
    45781 atggaggatt tggaagggat tctggggaaa atactctttg aatttcctgc cagggagata
    45841 aacttccata taccgcgctg gatggatacg ctggagctgg atcattggct taaacagggc
    45901 atagttgaca gcatcagaac gaatctcagt gaagtaaaca gaataagaga tattgacgat
    45961 gccataagaa agtttgacgg tgcagaaaac cttcaggctg tggaaatatc tgatatgcgc
    46021 cttggagaag gagtcgtgaa tataaccata agcctggacg acggactttt ctatagggtt
    46081 ttgggagagg tttcgggcta tagcctggaa ggagattatc agcttgtcgc attggtaaag
    46141 gagctagccc aatcaaagcg cgaatatgac aaagtcaagt cagccttggg ggatgtcaag
    46201 gaattcggtt atggcatggt agctccttcc gtggaggaaa taaagcttga agagcctgta
    46261 attgtgaagc aaggcagcaa attcggagta aggctgagag ccagtgctcc atcgctgcat
    46321 ataataaaaa ccgacataga aactgaaatt gctccgatag ttggaacaga gaaacaaagc
    46381 gaagaattca tcaagtactt tatggaggag tttgagaata atccgcagaa aatatgggaa
    46441 tccaatatgt tcggaaagtc tttgtacgat atggtcaaag aggaactgca gggcaagctg
    46501 tacaagatgc cggaggatat tcagaataag ctgcagctta cactgcagaa ggttgtcaat
    46561 gacacccggg gcagtttgat atgcataatc atatagagga gtaaaacaaa ataagggtgt
    46621 tctgattgat tgcaaaccag gacaccattt tttttaaaaa tttttgaaac ttttttgtgc
    46681 atttaacagt atttagatat agtgaatatt taagaaaata atatttgtga taaatttcaa
    46741 gagggggtta taatatgatg gaaagtataa aaagacttag aaatccttcg gagcttaaga
    46801 ttgccgccaa ggaaaatctt gatggaaagt ggcttatagc cattgctgta actgttgtcg
    46861 catggctgct gctggatgcg tttacatcaa gcaacggagg gaatgccgct tataagtata
    46921 taatagagaa cggaaaatct ataagggttc aagccgatgg aagtgcattt aaaaatatga
    46981 tgtcaattgt aagcttgata ttgggcggac cgatttattt cggagttgct tcttattttc
    47041 taaagcttgc gagatatgaa acggctgagt ttaatgacat gttttcaggc ttcagcttgt
    47101 ttaagactaa ttttgtcctg catttactga tgatagtttt cacagtatta tgggcattgc
    47161 tgcttattgt accgggcata atagctgcaa taaaatactc aatggccttc tatatattga
    47221 atgacaatcc ggaaatcgga gctatggaag ccatcagaag aagcaaagag atgatggaag
    47281 gacataagat gagattcgcc gagatgtggc tcagctttct cggatggttt ttattgggca
    47341 ttgtaacctt tggattggga atgatatatg cagttcctta ttacagggca gctaaagcta
    47401 atttttatct ggacttgaaa gaaagctaca gtgtttaaaa gaccctataa cacattaaaa
    47461 gtaacaggca tgagcctgtc actttttttt gtttttctta tgcttttttt ggatattgtc
    47521 agaagcgggg gccgatattg aatcggtgta tggaatcctg gtggtctcat ttctgctttc
    47581 atctacagcc ccggtgctgc tggcggcatt tactcctttt gaatgcttca aagcatcaac
    47641 tccttttatc tgatattcag atacactata ttttgcagct atttgcgaat ttttattcaa
    47701 aaaggacacg gggacgttcg ggacaggaga aacgtccccg tgtcccttgt gatattattt
    47761 tttatttttt gaaacaaaac gaggacaact gcagtatata attgtgaaga aagaaattca
    47821 cccggttcgt ggtacggggt gaattaatcc aattgacggg agcgaattat tatgagatgc
    47881 agaagaaatg agagagagaa gggtgattgc agcggccgcg gtgcagttta ctctttggtg
    47941 aaccttatat tcggattggt agctctcaca attaaaatga catttaaaat tgttgaagta
    48001 gtatttcaga ctttattcgg atttatttca ctttttgttt ccggatccag agattctcag
    48061 aaaactccaa acgtgtatga aaagccgatg cctgagaaga atgaagcacc cagcaatgtg
    48121 agagaattca agaaaccgga aagcagcaag gcacaagccg atgtcccgga agtcgaaagc
    48181 agtgatgctc ctgaagaggt taagaacaag gatttttatg tggtgttgtt catactcaca
    48241 attattcccg ttatagtggc tctggcaatg ggcaagctat attatgccgg aataataggg
    48301 gcagcaggct ttggacttat gatgctggtt ggaattgcag cctctgcata cggaaagggt
    48361 gcggcaaagg aaacttgtga ggatgaggaa gaagctgaag aggatgattc ggtagagagg
    48421 ctgataaagg atgcttttga aaagctcttt acgataagga aggatattgg aaagatacag
    48481 gacgcagata taagggcaag gcttgaagcc atatgctgta caggggagaa gataattggg
    48541 gaggttagga caaatccgga aggacttgct catgtcagga agtttttcta ttactatatt
    48601 gacgcttttg gtgaaatagt gaataagtat ctgaagctgt caagctttga ggagtcttcg
    48661 gaagaaatcg gaagactgat ggctgagaca gaaagatcct ttgccgatat agacgggatt
    48721 tttaaggatc tgtgcgaaaa gctgctggaa aaggatatga tgcatcttaa ggcagagatt
    48781 aatgtaatca agaacagcaa ttagagtttt gacaaatata ccggctgcag ttataattat
    48841 aaaggaaatg aatataatgt aatcttcagg gatgggtgaa attccctacc ggcggtaaag
    48901 cccgcgagcc ggtggtgcct agtgcatcat cagcagattc ggtgagactc cgaggccgac
    48961 agtaaagtct ggatggaaga aggtgcaatc tttatctatt tgtgcatatt tttgagccct
    49021 gaagtttttt tgcttcaggg cttagttttt ttctttccaa atatggaata ataaccagta
    49081 gtaaaaaagg aggagtgatt ggagtggaag atgcctctta catgaagagg gctcttgaat
    49141 tggctgccag aggcagcgga agaaccagcc ctaatccgct tgtaggtgct gtcattgtaa
    49201 aaaacggaag aattatcgga gaaggatggc atgaaagggc gggagagccc catgcagaga
    49261 taaatgccat tatgaattca agagaatctg ttgaaggagc cacaatatat gtgaatttgg
    49321 aaccttgctc ccacttcgga aagacacccc cctgcgccga ggaacttgtg aaaaggaagt
    49381 ttaaacgtgt ggttatggca atggaagacc ccaatccttt agtggcggga cggggtataa
    49441 agcttctcag ggaaagcgga attcaggccg atgtgggagt tctgaatgct gaagccttga
    49501 aattgaatga tattttcata aagcatataa cagtaaagga accctttgtg cttcttaaga
    49561 cagcaatgac tcttgacggg aaggccgcct caaggacggg agactccaaa tggataagcg
    49621 gtgaatcctc aagggcatat gtccacagct taaggaacag atattcatca atactggtag
    49681 gtgttaatac agtcataagt gatgatcctg aactgacctc aagaattgat ggagttcaaa
    49741 caagaaatcc cgtaaggata attatggatt caggagggag aataccgatt gatgcaaatg
    49801 tactgaacat agatgaaagc aggaggacga ttattgcagc cacagaggat atgacggaag
    49861 aaaagcgcag gcaattggag agcaagggtg cggaaatatt aattactgat aaaagaaatg
    49921 gaagggtgga tataaggcag cttttagagg aacttgcggg tagaggtatc gacagtctta
    49981 tgattgaagg aggcggtaca gtagcagccg cattcctgga agaaggttta atagacaagg
    50041 tggctttttt cattgcgccg gttgtaatag ggggcaaaac tgcaccgaca ccgctgatgg
    50101 gggaaggggt cagccttatt gaaaagggtt ataggctaaa acacagaagc atcagtgttt
    50161 ttggacagga tgtcctaatt gagggatata taaatgtacc ttgggagagg tgaattgact
    50221 tgtttacggg aattgtcgag gaaataggaa gaatacggaa cataagaaag ggggaaaaat
    50281 ctgaaagtct tattgtcgaa tgcaaaaata ttcttgaagg aactaagatg ggtgacagca
    50341 tcagtgtaaa cggcgtttgc ctgactgtcg cagatatgtc tgcaaacagc ttcgaagctg
    50401 atgtgatgcc ggaaacagtg agagctacga atttaaaata tcttaagccc ggggattcag
    50461 tcaaccttga aagggcgctt tcccccaccg gaaggttcgg cggccatata gtgtcggggc
    50521 atgttgacgg gacaggaaca atacaggaaa taaaaaggga ggataatgca atatggttta
    50581 ccatttctgc aaggaacgaa atactgaaat acgtgataca taaaggttcc gttgcggtgg
    50641 acggcataag ccttacggta caatatgctg atgagagggt gttcaaaata gcattgatac
    50701 cgcatacccg ggattacacc acactgccgg gcagaaaggt aggatactgc gtgaatattg
    50761 aatgcgacat aataggcaaa tatatagaga agttgatgaa ttacacaggg aaggacagca
    50821 agggcggtat ttccctggag tacctgctgg aaaacggttt ttaggaggaa tagacatgtt
    50881 ttgcagaata gaagacgctt tagatgaatt aaagaagggc aatatgataa ttgttgtgga
    50941 tgatgaggac agggaaaatg aaggagatct tctcatggca gctgaaaagg taactccgga
    51001 agcaataaat ttcatggcta tgcacggaag ggggcttatt tgtgtcccta tgaccgaaga
    51061 gaggcttaag gagctgaaaa tagaacaaat gacaggaaac agcaccgact taagagaaac
    51121 agcatttaca gtttcagttg atatcaaaag tgctgctacc ggaatatccg catatgagag
    51181 ggctgatacg gtaagggcat tagtgaatcc tgatacaaag ccggaggaat tggccaagcc
    51241 cgggcatata tttcctttaa aggcgagaaa gggaggcgta cttaaaagag ccggacatac
    51301 tgaggccgct gtggatcttg ccggaatggc aggtttttat ccggcagggg ttatatgtga
    51361 gataatgaag gatgacggaa caatggcaag gctttttgac ctttttgaat atgcaaagaa
    51421 acacaagctt aagatgatta ctatagcgga cctgataaaa tacagaagcc gtgaggagaa
    51481 gctggtatat aaagctgcag atgctttact gcctactaaa tatggggaat tcagggtaat
    51541 ggcttatgag agcaaaataa acggtgagca tcatatagca cttgtcaagg gagatgtggc
    51601 cgctgaggag cctgtattgg taagggttca ttcggaatgc ctgactggtg atgcctttgg
    51661 ctccgtaagg tgcgactgtg gtgagcagct ggcagaagca gcggcaatga ttcaggatga
    51721 aggcagaggg gtattgctgt acatgaggca ggaaggcagg ggaatagggc ttgttaacaa
    51781 gataaaggcc tatgagcttc aggacacggg aatagatacc gttgaggcaa atgagcttct
    51841 gggttttccc gcagacttaa gggactacgg cataggggcc cagattctta aggatctcgg
    51901 aataaaaaag ataaggctga ttacaaacaa tccaaagaag attaagggat tagcaggata
    51961 tggacttgaa gttgtggagc gggttcctat tgagatggat gcgaacataa ggaatgagaa
    52021 atacctgctt accaaaaagg aaaaaatggg gcatatgttc cataatatat gagaggaggc
    52081 aaaaaattga gagtatatga aggtaatctg acagccgggg atagaaggtt tgctgttatt
    52141 gcaggaaggt tcaacgaatt tataagcagc aagctgcttg ggggttgtat agacggactt
    52201 aaaagacacg gctgccccga gtccaatatt gaggtaagct gggttccggg tgcttttgag
    52261 ataccgctgg ccgcacaaaa acttgcaagg accggaaggt atgatgcggt gatatgtctc
    52321 ggagcggtta tcaggggttc cacacctcac tttgattatg tatgcagcga agtatccaaa
    52381 gggatagcca aggtaatgct ggatacagga ttgccgataa tttttggagt tcttacaact
    52441 gaaagcatcg aacaggctgt tgaaagggca ggaacgaaat cggggaacaa gggttttgat
    52501 gcagcaatga cagccattga aatggctaat ttgctgagta ccataggata agagtaatga
    52561 agtccacata aaaatacaga gaaataggac tgttgcacaa tatcggaata atgcgacgca
    52621 tcacaagagt ttgtaaagta ctccgactgt aggggaagta tgttctcctc actttccaaa
    52681 gccagcaaaa tcactggcgg gggtcccatc tctgcgggcc cgtctggtta ttttgccagg
    52741 ggtattcccg ttccgaggtc gggtggaaag tttcgtcaaa gatatcttcc ccacgtcggt
    52801 atttgcattt ccttaataca gcataattcc caaagggcac tggccttgta taacatatga
    52861 tgctctgtag gatgcatact aagcaagagc ttgtatagta tagcagggca cacttttatg
    52921 cattttgtaa caggcctctg atatggcttt ggcaaaaagt tcgtaaagcc ggcatggacg
    52981 ccggcttagg tgccacgggt catggaggac cttttggcac cggtagaact tttccaaagg
    53041 catagcagta taggcctgtc aaaatcataa tccgtgtgcc tcgatatact atactgtctc
    53101 ttgcggaagg ttatgcaaac ttcagatatg tcgcattctt ctacttatgc ggcttattga
    53161 agctatttca agttttcggg attaagtaca tccaattccg gaagtatgaa ccttccgtct
    53221 tttcttacga gtatgtcatc aaaccagatt tctcctccgc cgtattccgg ggtctgtatg
    53281 tttacaagat cccaatgaat ggcggaatta ttaccattga agcagtggtc ataagcgctg
    53341 ccgggagtga aatggaagct gcccatgatt ttttcgtcaa agaggatatc cttcatgggc
    53401 ttgattatat atgggttaac acccaatgcg aattctccca catatcttgc gccttcatcg
    53461 gtgtcgaata cctgattgat tctgtcggtg tcatttgcag aggcattgat tattcttcca
    53521 tccttgaatt ccaatcttat attttcatat gtatatcctt ggtattccga gggagtgtta
    53581 taagtaatat aaccgtttac agaatccctt acaggagctg aatagacctc tccgtcagga
    53641 atattggatt taccgtcgca ttttattgcg ggaagccctt tgatggagaa gcttaaatct
    53701 gttccagtcc ctatgattct gacacggtcg gtattattca tgagttctat cagcggatcc
    53761 atagccttgg acatttttga atagtccagg ttgcaaacat cgaaatagaa gtcctcaaac
    53821 tgctctttgc tcatattggc catctgcgcc attgaactgt taggaaacct cattatgcac
    53881 cattttgttt ttgccactcg gatattggta tgcactgtct tcgaccagaa ggattgataa
    53941 aaacccaact tttcactggg aatatcagcc cattcagaag agttgtaagc gcctcttatt
    54001 cctatatatg cctgcatatc cttcattctt tccgcttcat agtgcgcagt cctggacata
    54061 tgctccgcat cggtaccaag tatcaattct ctcatgagag atctgttttt tattgtcagg
    54121 aatggtttgc ctccaatctt gtagatttct tttacaagct ccgaagcaag ctcttcaccg
    54181 gagtcaaata cttcgataag gatattttca tttggtttaa gctggacgga ataatttaaa
    54241 aggttaattg ccatttgttt aatccgggga tctttcatta atagcgcctc ctgttaagtg
    54301 aattttaata tgtatattct atgtattatt taataatata ataatattat gacaggcttt
    54361 ttctccatta gacttcggtt acaaaacata atacaattat aaaatttgac atacataaaa
    54421 caatgctgtt attattatag ctttttggca aatagttgta aagtgggaat gccgtacatg
    54481 gggttatcat aaagtgtaaa acatataata tatgatgttt gagatgcagc atataaggag
    54541 atgaaagaat gagcgtcata gaacacgcct acggaaaaac cttcaagacg atattatggg
    54601 ctgtgaaccc tctgaaaaag gtattcaaaa agaccttgtg cgaggtccat gtatttatta
    54661 atgaaaaagc cgttgaaata ctcaggaatg acggatttca agatgcatat gaattctttt
    54721 ccataaacaa aggctgcctg aatgacgggg ccgtctgggc agatcaggat ttcaagagca
    54781 gggagcattt ctacaaccct tttacccaca gaggtttata tggctgcaag tcatcaaagc
    54841 atttgttcag gaaatattat ggttgcgcat tggtatactg ggactgcggg gataaggata
    54901 aagccatgtt ctacctgggt gccgccgtgc atctggtaca agatgccaca ataccgcagc
    54961 acggaagtgt aaaactgtta aagagccaca gaaaatatga acaatggatt cacaaggttc
    55021 atgatgattt cttgcattat tctgtttctg aaggcggaat atacctggaa aatccttttg
    55081 aatatattga aaaaaacgca aaagaagcct tggaagccta cacaaaatat tcacttatca
    55141 gaaatccctc agaaaaattt ttcaggattg caaacaggac attccctctg gctcagaaaa
    55201 ctacagcggg atgtctgatg aacttctatg ccaaaattaa tgaagggtga actccttaag
    55261 aggtacagcg gcgaaattga ttattttctt tgatgagctt ggcctcagcg gaaaaaacat
    55321 tactatttca tcctgtgaag gagtgtctgc aaatatgttg cggagaaata cctcataacc
    55381 cacctgcttc agtatctcac ggagggataa gtttttaaag tccttaaagg tatccatatc
    55441 tgtgagttta ttcttgatat atccatggat ttcttccttt attgctgcat ggttgaatct
    55501 gtttattgct gccggcatgc ttccgcttct gacatacagc agccaatcga gtttcatata
    55561 ttcgttcaat aagccgaact gctcattatc cagcctgtta acgaatgcat cttccgcaaa
    55621 gcacttcaaa acagtgtata attcattaac atttttcggg gcattgaaat ggcctttttg
    55681 attccaatag cctgacagcc tgcggtaaaa atcaaagggt tcagtttccg ctgcctgaag
    55741 cagaaagtcc agagtatgcc tgaacctgcc gctgttgtaa tactgctcaa gcacattttc
    55801 aatatccttt agctccatga tttctttaaa ggataaccag gcggtgctta ttacctcata
    55861 gggaggaaaa tcatgatgct gtatgcggta ttttttgcag ccttgtctta tgccggagcc
    55921 cttcaggagc tttaaaaaac ccagctgcag catgtcagga ttcaaagaat acacatcatt
    55981 gaaggatttt tcgaagcttt ccatatcctc atagggaagt ccggctatca aatccaggtg
    56041 tatatgggca ttcttaaagg ataaaagcct ggcaacatta tgactcactt tgtcaaaatc
    56101 catttttctc tttatttcgg acaaggtctt gggattggtg gattgtactc cgatttcaaa
    56161 ctggaacatg ccatccggcg cctttcccgc gatctcaaga aagcgcacat caatcaaatc
    56221 agcagctatc tcaaaatgga agttggttga tggattaaga tcaataatat actgcattat
    56281 ttcaatagat cgttccaaat cacagttgaa ggttctgtca acaagtttaa cctgctttat
    56341 tcctgcattt gcaaaacttt tgatatcctg gcgcactctg tccattgcca aataccttac
    56401 cccccgtatt gttgaagaaa ggcagtattg acaattaaag ggacagcccc tgctggtttc
    56461 ataatatata atcctgttat caaggccgtc aaagctgtca taaggaaaag gaagcatatc
    56521 caaatcagga atgtcaggcc tgggttcagt aatggcgaca gattctccgg agcgaaatgc
    56581 aatgcccggt atatccggca aattactttt gccggcagtg aggaagcaaa gcagatcaag
    56641 catggcagcc tctccttcac caatgacaat atattcaatg aaggaatatt ttgaaagaag
    56701 atctgctgca tcataggata cctcaggacc tccaagaatt attacagctt ccggcctggc
    56761 ttttttcaag ctgctgcata tatcgagaac catgctgatg ttccatatat agcaggaaaa
    56821 gccgtatata cgggcattgg agcagtacag cttcttcagg atgctgctga tgctgtcgtt
    56881 tatgctgaat tcggctatgt caattccttt tatatgtgct tcacaaaact ttttcagata
    56941 tctgaccgca agattggagt ggatatattt tgcattaatt gcacagagaa gaattccatt
    57001 tttcataata agcctccctt tgcaaatgtt tcgcacatgt attaccaaat gtaaattcta
    57061 aaatatatgg tataattata acagtaaaga tatgatatgc atatacacct tatttaataa
    57121 attgaagttc tagttcagac tggaggtatt cttatgagag ataatgatga tttgaaaagc
    57181 agagcaaaaa aaatttcgga aatattgttc aaaatctatc cggaagcaac ctgctccctg
    57241 gattatgata aacctctgga actgctgatt gctacgatgc ttgcggctca gtgcaccgat
    57301 gaaagagtga atattgtaac ccgggacttg tttaagaagt acacttcggt gtataattat
    57361 gcaagtgcaa atcctgagga actggaggag gatatcaggc ccacaggctt ttacaggaat
    57421 aaagccaata atattatcgg atgctgtaaa aagctgataa aagacttcgg aggcaaggtt
    57481 cctgacaata tggaggattt actaagcttg tccggagtcg ggagaaagac cgccaatgtg
    57541 gtattgggca acatatataa aattccgggc ataatagtcg atacccactg caaaagactg
    57601 tcaaacagga ttggattaac gaaggaggag gatcctgaaa aaatagaatt tgatctgatg
    57661 gagattctgc ccagggagaa ttggacagca tttagcaaca gcctggtata tcacggaaga
    57721 gcaatatgca atgccaggaa accaaaatgc gttcagtgcc atattgctca gtattgtgac
    57781 ttttttaaca atatttaaag ggaaaatgaa taaattggcg ttaaattaaa taattaatag
    57841 tatttgagga attttgaatt ttattaatag ctaaatatat attaaacttt taatgaagga
    57901 ttttgctgtt ttataacgaa atagacagca taagtttcaa tactgaaata ttgtttatcc
    57961 agcagtgtta ataatatcat tattgaaaga ggtggtgaag catttgacta cggatatcat
    58021 caaggggatt agggagacag aacttgcggc tgaggagaag atcaaagcag cacagcagaa
    58081 agccaaagat atattgatgt ctgcagagca agaggctgat catatcatta aagccgccgt
    58141 ggatcaggca attcaggaaa gtaaaaagca gctggaggtt gcagaaaagg aggccaatct
    58201 ggaggctgaa agcaagagga agcaaaatga cgaattatgt caggaactga ggcgaaaggc
    58261 tgaggaaaaa atggaagagg cagtgaattt ggtgatggag aggattgtga atatgaatgg
    58321 caatagttaa aatgaaaaaa gccaccatga tagctctcca gtctgaaaaa aatgagatac
    58381 tcaatgacct gcagagattc ggttatctgc aggtagtgaa ccttgaagag cgaatggaac
    58441 aaggcttcga agagttgcag cccgacagtg ataatgaagc tgtgggcagg ctggaggcga
    58501 agctttcgca agtaaaatac tccattgatt ttttaggcaa attcaatacg gcaaaaaagc
    58561 ctttgtttgc tcaaaaagtt caggtggatg aaacaaaacg caaagagtac cttgacaatg
    58621 aagaaaagct caatgagatt tatgaaagct gcagaagcat tgacagcaga tttacagaga
    58681 ttaaaagctc tgaaacaaaa ctgaacaatg caatagcgca gcttactccg tggctggcat
    58741 tgaaagaaaa acttgaggag ataggcacta ctcataatac caactctatt ctggggttta
    58801 tagcaagcaa atatgaagat gagcttagta ctgctgtaaa agatgaggat ctgactgcat
    58861 atctggaaaa agtaggcgta gagaaggaaa attcatattt tatggtgata taccatcaaa
    58921 catgtgaaga gagaatctct caactcctta agcaggtcgg atggacaaag gtaaccttta
    58981 gcgaatattc aggaactcct gaagaaaaca tcaataggat taaagcagaa atcagcaatt
    59041 tggagaacat aaaaaaagag ctggcaaaaa aagctgaagc aatggtacct gaatcagggt
    59101 tccttgaaat attatatgat ttgctttcaa tagaaaaaga cagacgcagc gtagtaaagg
    59161 atttcattaa aacggacaag acatttatgc tgcagggatg gataccggag aagtttgccg
    59221 atgatatgac gaagcttctg gatggtatta ccgataagta taccataagc tttgaagacc
    59281 caagtgaaga ggatgagata ccggtgcttc tgaacaatcc aaaggcggta aagccttatg
    59341 agatgataac tgacttgtat agtgtaccaa atgcaaaggg tatagatcct aatatcttca
    59401 tggcaccatt ctttgtggtt ttcttcggca ttatggtaac ggatgctgta tacggaattc
    59461 ttatgtcagc tttaaccggt tatgttctct ataagtacaa accccagggc ggaatgaaaa
    59521 acatgatgtg gattatgttc tttggaggta tttcgacatt cttttgggga ggcatcttcg
    59581 gaggctggct gggagatttg ataaaggtga agccatggtg gttcaatcct ttggatgagc
    59641 cgctcaagat gttgatattc tgtttgatcc tgggtgttat acatctgtat accggatata
    59701 tacttcaagc ataccaaaat atcaaagcag gcaattttat ggatgcaata tatgaccagg
    59761 tgctttggct tatgctgctg accgggctta tgttcctggc actgccgcct cttgctgcgg
    59821 ttggaaaata tatggcaata gttggtgcgg caggtacggt tatctttagt gccagatctg
    59881 aaaagaacat cttcaagagg ctgatatcag gtgtattggc tttgtataat gtcagcggat
    59941 ttttaggtga tgtattgtca tattcgagat tgtttgcatt atgcctggca acaggcgtca
    60001 ttgcccaggt ttttaatgca atgggcctta tgatgggtgg cagtataatt ggaaagataa
    60061 ttatgatcgc ttttctggct tttgcacatg tatttaatac agccctcggg gttttgggag
    60121 catatgtgca tacaagccga ttacaatacg ttgagttctt cggaaaattc tatgagggtg
    60181 aaggaaagcc ctttaatcct cttaggatca gaactaaata tatacaagag taatatcatt
    60241 attaagtatt ttttataaca aaagggaggc aatataaaaa tggaaaactt gattgaatta
    60301 atgggtaact caggtttatg gatggcattt cttggttcgg cattagcggc aggcctggca
    60361 ggctgcggat ctgcaatagg tgttggtatc gcaggtcagg ctgcggcagg agtaataact
    60421 gaagatccta gcaaatttgg taagacgctt cttttgcagg cacttcccgg aactcagggc
    60481 atttatggtt tgttgattgg atttcttatt tggtcaaaaa ttggtgtatt tggaacaata
    60541 gtacccgtta caatagagca agggcttatg cttatgcttg ttggcttacc ggttggcctt
    60601 gtaggactta tatctgctat ttatcagggg aaagcatcag ctgcaagctg tggaataatt
    60661 gccaagaggc ctgaagaatt cggaaaaggt atgatttatc ctgcaatggt tgagacttat
    60721 gctgttcttt cattgctggc atcattcctc atgcttaacg gcatcaaatt ataagggagt
    60781 ggcttaaatg gcaggagtag aaaacattaa ggagaaaatc ttannnnnnn nnnnnnnnnn
    60841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    60901 nnnnnnnnnn nnnnnnnnnn nnnttataag ggagtggctt aaatggcagg agtagaaaac
    60961 attaaggaga aaatcttaca ggatgcagat ttaagagcga aagaaatatt ggaaaaagcg
    61021 agactccaag ccaatgaaat tgtagaaaag gctaaacaga aagccgcatc cagagcaaag
    61081 gagatttcgc agaaatctgt ttatgatgtc agtgaaaaga aaaggataat taattcaatc
    61141 gttgagcttg agatgcgcaa ggatgtattg gctgcaaagc agaaggcaat aggagaggtt
    61201 tttgataagg ttcttgagag aatgaacaac ttggaagaca gcaggtatga gaaagtaatc
    61261 atagacatgt tggcggcttc ggctgaaagc ggagaagaag agattgtctt ctctgaatca
    61321 ggcagaaaaa agctttcttc agattttctc aataaactga ataagaccct tgcagctgct
    61381 ggaaaaaagg gcaatttaaa aatatcggat gaaaccagga atatttccgg aggcttcatt
    61441 ttgaagagcc agggagtaga gattaataac tctttcgagg caatcataag gctctataga
    61501 gatgaaattg aaccgaaggt agctgagatt ttcttccggg cataataatt atgcagaact
    61561 cgttaactgg aagaaaggag gataagctca atgagtgaaa ataaatacct atattctgtt
    61621 accagaatac gagcactgga aacgaagctt cttgacaagg caaaaataga gagaatgata
    61681 gaagccagga acgcagatga aattattaag atactgtatg aaacagaata tgcaaactca
    61741 atttctgaga tgaagagtgt tgaagactat gaatcagtgc tctccaagga gctttctaat
    61801 acctataagc ttctttcgga aatatctccg gttccggaac taacaaatct gtttcagctg
    61861 agatacgaca ttcacaattt gaagactctt ctgaaaagca gttaccttga tgaggaaaat
    61921 gacgaattat tgagcgaaat tggcactata ccgattcagc agcttaaagg aatgatcaaa
    61981 gagaaggttt tcagtgattt ggaccccctt gtaagagaat ccttggaaga agttgtggga
    62041 gagttcacgg ttaaccccga tccgcagcta attgatgtga ctctggacaa ggctctgtat
    62101 aaactgatgt ataaagcggc aaaggataac aagagcagct ttttgacgga ttatatatcg
    62161 acacagatag acctgataaa tatcaagtcg ctgattagag taaagtcaat gggctatggg
    62221 cgggaattcc ttaaaaaagt aatacttgaa aacggaaagc tggattatgc attcttcagc
    62281 gatatctttg atgaatcctt ggaaacactg attgacagat tggcctacaa ggattacgga
    62341 aaggtagtgg aagaaggtat aagcaactat attaagacga aatccctgac taagtttgaa
    62401 aagctttctg acgattttat atttgaactc gcaaagaaag gcaagtttgt tgcatttgga
    62461 atcgaacccc tgattggtta tctgatggcc aaggagaatg agacaaagat tatcagaatg
    62521 ataatggtcg gaaaaatcaa tgagattccg aatgaattga taagagaaag gctacgtgat
    62581 gtatatgtat aagattggag ttattgggga caaggactcg atactgggtt ttaaggcttt
    62641 agggatatcg gtatttccga ccaacgatat aagagaagcc aacagattga ttcacaagct
    62701 ggcaagggag aattacgcag tgatttttgt aacagaacag cttgccaagg atctggacga
    62761 agcaatcggt gaatataaga attccttgac tccggcaata atcatgattc ctaataatag
    62821 gggaactttg ggattaggga tagacggcgt caaaaaatca gtcgagaaag ctatcggttc
    62881 agatatatta ttcgggaaag aaggttgata acttgagcga tggtagaatt ataaaagtat
    62941 caggtcctct tgtcatagcg gaaggcatgc tggatgcaaa gatgtatgac gttgtgcgcg
    63001 ttagtgataa aaatctcata ggtgaaataa tagaaattag aggcgataaa gcttcaatac
    63061 aggtatatga ggaaacctca ggcttagggc cgggagaacc ggttgttacc accggtgagc
    63121 ctcttagcgt tgaactggga ccggggctta tcgagtcgat ttttgacggt atccaaaggc
    63181 ctcttgacgt agtaagaagt gcatccggcg accatatagc cagaggcgta caggtagaaa
    63241 agcttaacaa gaccagaaag tggaattttg aaccttcggt gaagcaaggc gacgctgtaa
    63301 aaacaggaga tattataggt atagttaaag agacttccat agttgttcat aaaataatgg
    63361 ttcctcatgg aattgaagga accatagagg aaatttattc cggcgagttc accattttgg
    63421 aaacagtggc aaaaattaag acaaaagacg ggataaagga agtacagatg ctccagaagt
    63481 ggccggtaag aaagggcaga ccatataagg aaaaaatgtc tccctcaaca ccaatgacca
    63541 caggacagag aataattgat acattcttcc cggttgcaaa gggcggaacg gcctgtatcc
    63601 ctggcccttt cggaagcggt aagacagttg tacagcatca gctggcaaag tgggcggatg
    63661 cagagatagt tgtttatata ggctgcggtg agcgtggaaa cgagatgacc gacgttctta
    63721 tggagtttcc ggagcttaag gatccaaaaa ccggagagcc gttgatgaag cgtacggttc
    63781 tgattgcaaa cacatccgac atgccggttg cagcccgtga agcaagtata tataccggca
    63841 taacaatagg cgaatacttc agagatatgg gttattccgt agctctgatg gcagactcaa
    63901 cctcccgttg ggcggaagca cttcgtgaaa tgtccggacg tctggaggaa atgcccggtg
    63961 aagaaggtta tcccgcatac cttggttcta gagcggcaga gttctatgag agagccggaa
    64021 aggttgtatg cgttggaact gaagacagag aaggtgccct tactgcagtc ggtgcggtat
    64081 cacctcccgg aggagacttg tcagagcctg taacccaggc aaccctaaga atagtaaagg
    64141 tattctgggg cctggatgct gctctggctt acagaaggca cttcccggca atcaactggc
    64201 ttcagagtta ttccttatac cttgacagat tgggtgattg gtttacagag gaagtggcgc
    64261 ttgactggaa caacctcaga atggaaacca tgagattgct tcaggaagag gcggaactga
    64321 acgaaatagt gaggctggta ggttttgatg ccctatcatt aaaagataga tttacattgg
    64381 aagcatccaa gagcataagg gaagactatc tgcatcaaaa tgccttccac gaaattgaca
    64441 catatacatc aatccataag cagtacagaa tgctgaaagc aatactgctg ttctatcacg
    64501 aaggacaaaa agcattgagc gaaggcgcat tctataaaga gcttactact cttccggtca
    64561 gggaggatat tgccaaagcc aagctcatac ttgaaaatga ctacgaatcc tttgatgttc
    64621 ttgaagctaa cattagagat cagatagctt ccatcctttc gaagggaggg aaaatagtat
    64681 gctaaaggaa tataaaacca ttaaagaagt agcaggaccg cttatgtttg ttgaccaggt
    64741 agagggcgta aagtacgacg aactggttga aatcgaatta aaaaacgggg agatgcgccg
    64801 cggaaaagta ctggaggttt ccggaagtac agctcttgtg cagctgtttg aaggttccac
    64861 aggaataaac atcaatgagg gtaaggttcg ttttacggga cacagccttg agctgccggt
    64921 gtctacagat atgctgggaa gagtatttga cggcttgggt aggccgaagg acggagggcc
    64981 caaaatcata cccgaatcaa ggaaagacat aaacggtaac ccgattaatc cggctgccag
    65041 agactatccc tcggagttca tacaaaccgg aatctctgct attgacgggc ttaatacact
    65101 ggtaagaggg cagaagcttc ctgttttctc aggctcgggc cttcctcacg caagacttgc
    65161 agcccagatt gcaagacagg caaaggtttt gggtactgaa agcaaatttg ccgttgtgtt
    65221 tgctgcaata ggtataacct ttgaagatgc ggattatttc gtatccgact tcaagagaac
    65281 cggtgcaata gacagggcag tgcttttcat gaatcttgca aacgacccgg ccatcgagcg
    65341 tatagcaaca ccgcgtatgg ccctgacagc agcagagtac ctggcatatg agaaggatat
    65401 gcacgtgctt gtaataatga cggatataac aaattactgc gatgctcttc gtgaagtatc
    65461 tgccgcacgt aaggaagttc ccgggagaag aggttatccc ggatacctgt ataccgacct
    65521 tgctaccata tatgaaagag cagggaggat tagggacagg aagggatcaa taactcagat
    65581 accgatactt tcaatgcctg aagatgacaa gacccatccg atacctgacc ttacgggata
    65641 tatcacagaa ggccagataa tactcagcag agagctttac agaaagggta taatgcctcc
    65701 tatagacgta cttccttcac tttcgcgtct taaggataaa ggtataggca gaggaaagac
    65761 aagagaagac catgccgata caatgaacca gctttttgcc tcttatgcaa gaggtaagga
    65821 agccaaagag cttgctgtaa ttcttggtga aggcgccctt agtgatacgg ataagctttt
    65881 tgccaagttt gctgatgaat ttgagaataa atatgtgtct cagggagata gtgaggacag
    65941 aagcattgaa cagacactta cactgggctg ggagcttctc tccatactgc cgaaagctga
    66001 acttaagagg atcaaggacg agtatattga taaatacctg cctaaagggg atgcatagca
    66061 tatggctaaa ctaaatgtta atcctactcg tatggagctg accggtctta aaggcaggct
    66121 gaaaactgcc aaaagaggcc ataagctgct taaagacaag caggatgann nnnnnnnnnn
    66181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    66241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa gaggcttcaa gcatatatcc ctacggcttt
    66301 gcagctacct cgggagagct ggatggggca atattctccc tgcaggaggt ttttgacaaa
    66361 ctgattgagc tggctcaggt ggaaaagacc tgtcagctta tggccgatga gatagaaaag
    66421 accagacgca gagttaacgc cctggaatat gtcatgattc cgcagctcat tgaaacagta
    66481 aagtatatta ccatgaagct agacgaaaat gagagaggaa gcagagtaag gctcatgaag
    66541 gtcaaggaca tgatggaaaa gcagaaagcc gaggcataaa gcaatccgca ggaacattcc
    66601 tgcggatttt ttctattgtt ttgcttctac aaaggtagtg cagcaggttc cttcactgga
    66661 atgggcaaca ccgtcaccca tcggattcac ttcaatcaca ctggcgctgc agtagttatt
    66721 gtcataatat gagcaggtgt ctacattgca ttttatgctg tgattgtggg catgcatttt
    66781 attggatgaa atgtcgttgc tgcttatatt tttatgcata aaaaagcctc cttgtaagtt
    66841 tatttaatat atatttttcg cagtattaga atataaatac cgaactaaat gtgttaaata
    66901 gtggcaggtt caatccggaa gagtgaacag tcagacttta gacacatgca cttaaaaaat
    66961 tttttgacat gttcaaaaaa ttttattaat aaaatgccaa atgatatgga aatatgtatt
    67021 aatttaatgt aatatatagg tggaggatgg atacaagatg gcggtaaaat tatgggggtg
    67081 ttttcaaaaa aatgggaatt caatttgcta acaggatgga caacttaaaa ggttccgtaa
    67141 tcagagagtt gctgaagtat gccgaacagc cggacataat atcatttgca ggagggcttc
    67201 cagcgcctga gcttttcccg gtggaaggaa tgatgaaagc tacggaaaga gtaatgatgg
    67261 aagatggaaa agcttcattg caatatggtg caactgacgg atatttaccg ctaagaaaga
    67321 aaatagctga aaggtctaaa aagattgggt ttattccgga tcccagtgaa atactcataa
    67381 catccggatc acagcagggg cttgatttca cggcaaaaat atttataaac aaagatgatg
    67441 taattataac cgaaagccca agctacctag gtgctttaaa tgctttcaag gcatatcagc
    67501 caaagtttgt agagattcct atggatgaag acgggatgat aattgaagag ctggaaaaag
    67561 ctctggcagc caataaaaat gtaaagttta tttataccat acccgatttc cagaacccga
    67621 caggaaggac aatgcctgtt gaaagaagaa aaaaacttat ggaattggct actgagtatg
    67681 aaatacctgt aatagaagat aatccatacg gagaattgcg gtttgaaggc gaaatactgc
    67741 cgtccttaaa gtgctttgat cccaagaacc ttgttattct tctgggaacc ttctcaaaga
    67801 ttctggcacc gggcatgagg cttggatggg tcatagcccg ccctgatttg ttgataaaat
    67861 acaatttggt caagcaggga gccgacttgc aaagcagctc tatggctcag agacaggcaa
    67921 atatgtttat ggaaatgtac gatattgaaa agcatatcga aaaaatcatc aaagtatatt
    67981 ctgcaagaag gaaccttatg cttgatacaa taaaaaagga atttccgtca aacgtaaagt
    68041 gcactaatcc ccaaggcgga ctttttgcat gggctacctt ccctgaagag gttgatgctt
    68101 ccgtgattct caagaaagct ttggaacaga aggttgcgtt tgttccgggc gagcctttct
    68161 atcccaacgg cgggaatgca aatcactgca ggcttaacta ttcatgcatg agtgaagata
    68221 agatagtaga aggaattgca agacttggca aaactttaaa atcaatgtaa acagcattaa
    68281 aaggctccgg gatatcccgg agctttttaa tgtaattggc attcacacag caaaggcagg
    68341 aatcggccgt taatatattt gaattgtaac aaatttggta ataataaatg tatatatggc
    68401 aaaaacacaa atataatagt ataatatgca taaccttatt agttgcatac ataaacatat
    68461 tagaaaagga ggaaaattga tggaaaaatg gacttgtaca gcatgcggtt atgtttatga
    68521 cccggaagca ggagatccgg acaacggaat tgaaccaggc actccttttg aagatattcc
    68581 cgaggactgg gtatgtccgt tgtgcggagt tggaaaggac atgttcgaaa aggaataaaa
    68641 gaagaaatat tatttagttt gcacgagaaa aactgagcaa gcctcagttt ttctattgta
    68701 aacaattatt ataaaaaaat tgtgtattgc taaaaatagt gatacatgat aaaataaatt
    68761 ttgtcggtaa ttccgaacta atattcccgg tttgtgtaat ggtagcacaa ctgactctgg
    68821 atcagtttgt tgaggttcga atccttaacc gggagccata cggtattaat acctgcaagg
    68881 ttttagactt tgcaggtttt tgttttttga ggtgttttac ccctacctta ccccaacccg
    68941 gaacttaagc aataaaaaac cgctcatttt gtgagcggca tatataataa ttattatata
    69001 tcaaatatat tattcagttt ttccatagct tcttttttct tattcggaag aacatggcta
    69061 tatgtatcgg cagtaatgac tatcgaagaa tggccgagta acacttgcat tactttaagt
    69121 tctattccgt tttcaagtcc tcgtgttgca aaggtatgac gcagacaatg cagattggca
    69181 tgggctattc ctgctttttt taatatacga taaaatactt tcgaaaaatt tggaggatct
    69241 atttctttcc caaggtttgt gcaaaatact agatcattat cattatataa tggcccagcc
    69301 aataattttt ccttggcttg ttcggctttg tgagttttaa gtactttaac catcgattcg
    69361 agtaaaggga tttgtctaat accactttcc gtttttggag tataatgacc ccacttgtca
    69421 atacagaatt ttttgcagtc ttagttaggt ctcctttctt tatgtaaatt atttacttta
    69481 accatattca taatgtcgtt gttgttaaaa ctgcacgttt tagtacaaca gtttggggat
    69541 gtcaagggcg agcgatgagc gagttcatct tgacctttga cagccatagg cataaaatcc
    69601 tgttgtttac gggcatttta catgccagcc tgccttccgg cgaggacggg gtttggggca
    69661 gggcctcata gcatctcaca ctgctttaac agttcatcct tctccttttc ataggactgt
    69721 ttggcctttt ctgct
//
LOCUS       DAOOAB010000009        61264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_584_length_61264_cov_15.977667, whole genome shotgun sequence.
ACCESSION   DAOOAB010000009 DAOOAB010000000
VERSION     DAOOAB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 61264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61264
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_584_length_61264_cov_15.977667"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1347
                     /locus_tag="PKU88_03485"
     CDS             91..1347
                     /locus_tag="PKU88_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPX68378.1"
                     /translation="MYLKETLKNGIRIVAEEIPYVNSVSIGLWFKVGSRYESIENNGI
                     SHFIEHMLFKGTKNRSAKEIANSIDKLGGQLNAFTSKESTCLYAKVLDTHFDIALDVL
                     ADMFFNSNFDCDEIDKERGVVIEEINMYEDSPEDLVHDLFSQSVWSGNPLGMSILGTE
                     ESLENLNRDIIVRYFKENYSPKNIVISVVGNFKHKDAFDKIKAYFEKAELINRSATDI
                     AVPRFEPEYTLKSKDTEQVHLCMGFNGVDIKNKAFYPMLVLNNVFGGAMSSRLFQKIR
                     EDRGLAYSVFSYPSSFEDCGLFSIYAGSKPDNLKSVAKLIMEEINMIKDKGITEEELY
                     DSKEQLKGSYILGLESTSGRMISIGKSELLLDVIYSPAEILAKIDKVDMDNVNSTIKH
                     IFDTDSMGAAVIGNMKNGTDIRNLFK"
     gene            1367..1825
                     /gene="dut"
                     /locus_tag="PKU88_03490"
     CDS             1367..1825
                     /gene="dut"
                     /locus_tag="PKU88_03490"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPX68379.1"
                     /translation="MKDVTVKYIRTSPDENPEPKIPAYTTEGSAGMDLSACISEDIII
                     KPGEIAMVKTGIAIQIPDSSIGGFVFPRSGLSSKHGISLANCVGVIDSDYTGEIICPV
                     INHGSSDYTIKNGDRIAQIVFMPVVNVRLQKADKLEATGRGAGGFGSTGK"
     gene            1893..2141
                     /locus_tag="PKU88_03495"
     CDS             1893..2141
                     /locus_tag="PKU88_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HPX68380.1"
                     /translation="MRFSKLGGKEIVSLPDCERLGYLGDCDLMIDGETGRIKSLIVPE
                     NKGTLSFFIDRRYIEIPWERIKKIGNDMIIIDFADILK"
     gene            2347..3225
                     /gene="dpsA"
                     /locus_tag="PKU88_03500"
     CDS             2347..3225
                     /gene="dpsA"
                     /locus_tag="PKU88_03500"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HPX68381.1"
                     /translation="MKSSRYLIIGGDDRLVELAKLFEGEGTDIATYGMDMTVIKNVPN
                     YLSFDEALENSDIVIGPIPFAKDVHKINSKYSSVDIEAEELFSKLGKDKKLILGAINN
                     YSKGLAEKYGIEYWDYHNDEGYQILNAIPTAEGALSILVNETKTTLFGSRILILGYGR
                     IGKLLSEYLKALGASLYVEARKDSDLSWITAERMKAIPLKELPQYLGEMEIIINTVPA
                     MLLDSNMLDLVKQEALILDLASAPGGTDFDCASKKGIKAIHAQGIPGKAACKSAAEYI
                     YSTIKKYLCADRQKGV"
     gene            3228..3542
                     /locus_tag="PKU88_03505"
     CDS             3228..3542
                     /locus_tag="PKU88_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HPX68382.1"
                     /translation="MKVEGLNIGLAVTGSFCTFDKIIPEAEKLIKQKANVYPIFSTNA
                     ASMDTRFGKAEDWLRRFEEITGNSVIKTIEDAEPIGPGNMLDVLVIAPCTGNGFTISK
                     LR"
     gene            complement(3520..4629)
                     /locus_tag="PKU88_03510"
     CDS             complement(3520..4629)
                     /locus_tag="PKU88_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPX68383.1"
                     /translation="MADKESLRAALYIRVSTEEQALDGQSADAQAETLKQYCIAFGIS
                     IYDYYMDLGVSGKSMKDRHELKRLIEDCKSRFFDIVLVWKISRLSRNLKDLLYLIDIF
                     ENNGVCFASCSEKFDTSTPIGRMTLQLLGSVAEFERNTIVENVKLGLKEFARKGGKSS
                     TVLGYDNIDKKLVVNEEEAGIVRLIFRLYAEGGMNCSAIAGYLNSMGLKSKRGCEFRG
                     SSISYILHNTVYIGINRHLIGKENQYSVKGTHDAIIDDELWNKAQKASTGTRKRTSRK
                     ASDYSGMQLKAFCMKCGMPLTSFHASSKGKKYIYLRCRNCSNYVNAEKLTKAVLEAIE
                     PITGKKEPSMHRQLPELPIKRIEAYKGDVNVVLKL"
     gene            4812..6137
                     /locus_tag="PKU88_03515"
     CDS             4812..6137
                     /locus_tag="PKU88_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HPX68384.1"
                     /translation="MSIIDTFLLRYAKEYDFYNELAHQVAMICETIIHRSGIRAIVTY
                     RAKKPESLKDKLMKRNAIKKYQSIEQIYRDIVDLSGVRIAIYFPGDREEIGRLIENEF
                     ITRKTKKFPNSEQKQHQDNPFKRRFTGYDAVHYRVKIKEEKLEENNKRFAHAQVEIQI
                     ASALMHAWAEVEHDLAYKPQIGKLSEEEFNILDELNGLVLSGDITLERLQKSFKQRIA
                     SMEQQFNNHYELAALLREKISGSFPEGCFIGRVDILLKFLQKIGMDNPKEIHMYLDSF
                     DKMKESVGSKTIVDCFADLILNHYPDYYRQYVEAKFESSFNNPYISIQKQMLLGKENE
                     QLNSFVEKWILFENAIKALEQKNITQSRSISDIIRRHLTADEIILDTVKNLEKTRNHI
                     IYSSDIPDNLQLRNAADSITLILNKLAELASDENKTVICKALDKIIKEF"
     gene            6155..6466
                     /locus_tag="PKU88_03520"
                     /pseudo
     CDS             6155..6466
                     /locus_tag="PKU88_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
     gene            6481..7722
                     /gene="dapG"
                     /locus_tag="PKU88_03525"
     CDS             6481..7722
                     /gene="dapG"
                     /locus_tag="PKU88_03525"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPX68385.1"
                     /translation="MKIIVQKFGGTSVSTQERRELVVDKIESAVKKGFLPIVVVSAIG
                     RKGDPYATDTLINLAASAGVKNNPRELDMIMYCGEIISCVVLSNTIKKRGYDNIVLTG
                     GQSGIITDEKFGDAEVLRVEPHYIKECIEKGLIPVIAGFQGISEKGEITTLGRGGSDV
                     TGAIIGEALNAEAVEIYTDVEGVMTADPKIVPDAAVIGTIYYNEVFQMAEYGARVIHP
                     RAVEVAMRSNLPIFIKNTMSDSSGTLITNCDKNRRYRYDKNDKLLTAVAHVGGRTRVR
                     ILFDGSAKEYENDSHLFSTIASEGISIDMINVNPDFKLFIIVDDDVEKLGTVLNRCGY
                     NYELKKDCSKVTIIGNKMRGVPGVMAKAIRALAKHNIEILQTSDSHTTISCLIESINT
                     NTAVNALHHEFELGKPAIQNL"
     gene            7923..8630
                     /locus_tag="PKU88_03530"
     CDS             7923..8630
                     /locus_tag="PKU88_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68386.1"
                     /translation="MDKIIQLKNIRKVYRMGNEKVIALDNISFEIEEKEICCLLGTSG
                     SGKSTLLNLMAGLEKPTKGEVKIKNYNLESLNEKQLAKLRQKYIGFVFQSYNLLEHFT
                     ALENVTLPLIFKGVPKPVREKKAKALLKAVGLGDRMNHKPSQMSGGQQQRVSIARAFI
                     SNPEIVYADEPTGNLDTRTSAETMDLITGMAEEYKQTLIIVTHDLEIAGYANRIIYIK
                     DGNVEKIDMLSEKAGRG"
     gene            8632..11016
                     /locus_tag="PKU88_03535"
     CDS             8632..11016
                     /locus_tag="PKU88_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68387.1"
                     /translation="MKRKLFIVFLAAIIAFAGLPAINVSAGIYDDIEVSYDVNESRIL
                     DGDSFTLYFTIDEVRAGEISDVEINIETGSNFKVLGGNLNIPAAVLDKQYNVNLQYLG
                     GSNSLPIKIKYDKNSVYEGEFIYNVEIAEIDEESVNPSSSSSDNEPKLIVESSSIPVA
                     AAGETMKIPITVTNESRYSAKDVTVKLSLANNDNNPFEISSLNLVQSIGSLNAKKSDG
                     LIFSLKVNPYAEEKTYELDVQFSYYNSKNNLFTSNEKIYVKVTNSKNEPVLMIQDIKH
                     STYPVNAGAKFQISFNVANVGGIDARGAKVSIEGLNKDGFTLYSGVNSWYFDNIEGNG
                     KKNITVDLVAADSMESGNYGLEIKMEYKDQRNNSYTASSSFFVTVKGEAKSSNVTIKN
                     ISVLPQEVRANENFTLSFDVLNEGKSDARNVKLTVKGDEGIISKSPDVRIIENLSAGE
                     QQKLEYVLYANNETKTQNYNIMINLEYEDADKENSKHSVNQYAGVYVNGGNSKITPKI
                     IISNYNFEPKLVRAGEEFSMDLTFMNTSSTKEVKNIKIYLTGIDSDKEGNVVFTPVGS
                     SNTFFVDAIKPKGTAVRSITMYTIPDAQPKYYNITANIEYQDDEGTEYKAEELIGIPV
                     IQQSKLDASEIGFPPETFIGQPIPVSLQYYNKGKTRLSNLMIKIEGDFRIENGEAFIG
                     NFESGSSDYYEAMVTPMKPGPAKGKVVFSYEDPSGDEFTYEREFSINAIEMPPMEMPG
                     GEMPMPPVSTKDKIMKYTIKNWFFWVCIAVIAAGIFTARRLIRNKRRKDMDLDE"
     gene            11009..12373
                     /locus_tag="PKU88_03540"
     CDS             11009..12373
                     /locus_tag="PKU88_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68388.1"
                     /translation="MNRIDIVRIVLKNLLRRKMRTMLTVMGVVIGTASIVIMVSIGIG
                     MNEGFKSEISRMGSLNSINVRSPETGMYYIYDETEAGRTNSTEMRDLDDAAVADFSRI
                     AGVESVIPFIETHGKIKLGKYIGYVQLQGINPEHMDKLGLKVTEGRLLGAGDKMNTVF
                     GFGVKDNLYNPKGSQRRRFGRNTPEVDLLGERITLSLDMSYGEEFIQRDTSVQKKPKT
                     YKINGVGLLEEGSYEYYSSIFMDITELKKIVKDFNKGKSKNDNSGDYRDIKGYSRIIV
                     KVKDINDVQRIKNQIKDLGFFADSLTDILESMQKTTSSIRIVLGAIGAVSLLVASIGI
                     TNTMVMSIYERTREIGIMKVIGSSLGDIKRMFLFEASMIGLLGGLIGLLLSYGVSYGL
                     NTAGFALIGFFGPSGGGSKPSIIPLWLAVSAMLFTTIIGLVSGFYPAHRAMNLSALEA
                     IRKE"
     gene            12496..13212
                     /locus_tag="PKU88_03545"
     CDS             12496..13212
                     /locus_tag="PKU88_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPX68389.1"
                     /translation="MTEADGADKEKEAKNNANENIKELGEVRVPQFRSNIHCITIIGQ
                     IEGHMVLPPQNKSTKYEHIIPQIVAVEENPEIEGLLLILNTVGGDVEAGLALSELIVS
                     MKKPSVSLVLGGGHSIGVPLAVSATHSYIASTATMTIHPIRMNGLVIGVPQTFDYFNK
                     MQERIIRFIVEHSNIDVDTIKRLMFTTSELANDIGTVLVGAEAVKYGLIKEIGGLHQA
                     LAKLQNLIAASKAAENQRSN"
     gene            13373..15607
                     /locus_tag="PKU88_03550"
     CDS             13373..15607
                     /locus_tag="PKU88_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPX68390.1"
                     /translation="MSGKRKENDLNYEISGVSLILASIYIYISLFSSNSGIVGEKVRL
                     FFLGTFGLGAYIVPVLIIILGLALVYRKNEIRINIKFFSFCIFLLSTLIVLFLSNYTE
                     IENADAKWLDAVRYYFSLGILNAGGGAVGALFGYAFVRLFGMIGTIAIVSSLYIISIL
                     MFTEVSSMRILQYIKQSASDIYSRLRNKNKEAAVTKELQRKIPVKHHNDIESSEADDE
                     ERIKNIDKKIKILDFTKRFDKDFKGTAENEADDKARNKDKTSRENIPSIVPVSNIHYV
                     NYKLPGTGLLALPAENKSGNGKKEVMNNVKILEDTLKNFGVSASIAQVSVGPTITRYE
                     LQLSPGVKVSKILSLADDISLSLASPDIRIEAPIPGKAAIGIEVPNKDITSVLLREVL
                     ESEEFRKHKSKISFALGKDVAGANIVADLAKMPHLLIAGSTGSGKSVCINTLIASILY
                     KATPEEVKLLLIDPKVVELSVYKNIPHLLVPVVTDPKKAAGALNWAVTEMTERYKKFA
                     ANNVRDIYGYNNLNKEELAKMPQIVVVIDELADLMMVAPSEVEDSICRLAQMARAAGI
                     HLVVATQRPSVDVITGLIKANIPSRISFAVSSHIDSRTILDMGGAEKLLGKGDALFYP
                     VGAAKPVRVQCAFVSEKEIEKLVAYIKEQVPASFDNKIIKEIEQSVEIDEDAEADELL
                     NDAIRIVVENGQASILMLQRRLRIGYSRAARLIDQMEERRIIGGYEGSKPRKVLLGRE
                     DLED"
     gene            15629..16966
                     /gene="rimO"
                     /locus_tag="PKU88_03555"
     CDS             15629..16966
                     /gene="rimO"
                     /locus_tag="PKU88_03555"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPX68391.1"
                     /translation="MSLKVAIISLGCSKNLIDSEVMVKSILNNDYEVTDNANIAEVII
                     INTCGFIESAKQESIDTILEMSDYKKNGRCKVLIATGCLAERYRDELIKELPELDAVI
                     GTGDYKDVPEVISKALKGEKVVLYGHQELIDIDKLPRHISGFGASSYLKIAEGCDNRC
                     SYCIIPALRGRYRSRKTEDILEEAKELSRNGIKEVNVIAQDITRYGLDLYKSRQLSEL
                     LKKLAEINGIEWIRLLYSYPDEFNDELIEVIAREDKICKYLDIPIQHTSNRILKKMGR
                     RSSREQIQRLIEKLRKRIPGIVLRTTLIVGFPGETEEDFKELYDFVSETKFNRLGVFA
                     YSREEDTPAFNMPNQVEEAVKQERLEKIMLLQKDISLEYNRKLVGQTLKVLVEGASDN
                     EYSGRSCMDAPEIDGKVYFKSNKEIIPGDFCSVTIKKAYEYDLMGERTDEPGK"
     gene            16950..17492
                     /gene="pgsA"
                     /locus_tag="PKU88_03560"
     CDS             16950..17492
                     /gene="pgsA"
                     /locus_tag="PKU88_03560"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPX68392.1"
                     /translation="MNLANKITIIRILMIPVFLLFAVAEFPYHMTAAVAVFLAASLTD
                     KLDGYIARKYNMITDFGKFMDPLADKLLVTAAFLVLIQLGRIEAWIVFIILAREFAVT
                     GLRTLAAAQNIIIAASSYGKIKTVTQIIAIVILLMDNYPFSLLNLPVDAVMTYAALLS
                     TVISGIDYFKKNMQVITRMK"
     gene            17515..18750
                     /locus_tag="PKU88_03565"
     CDS             17515..18750
                     /locus_tag="PKU88_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPX68393.1"
                     /translation="MKSEIIAVGTELLLGNIVNTNAQYLSQKLADLGIDVYYHVVVGD
                     NLKRLTETIKTSLERSDIVITSGGLGPTADDITKEGAAQAMGLKLLPDEESIERIKKM
                     FSSTGRIMTENNIKQGYIPEGAVVLENNNGTAPGVLIEKEGKIVIMLPGPPKELYPMF
                     ESKVLPYLKSKTDSTIRSKVLRVIGVGESAVEHILKDIFDSQANPTIAPYAKDGEVHL
                     RITAKTGIPEEADSLIAEMEQKVRAILGDNIYGCNEETLEEAVLKLLQKKNLTISLAE
                     SCTGGLVASRLTDIPGASASLISGVISYSNESKINMLKVKEETIRKYGAVSPQTAEEM
                     AVGAKRLSNTDIGLSITGIAGPDGGSVEKPVGLCYIGIAIGKSVNVQKIMLTGNRNRI
                     RWGSSSRALDFLRRELLSG"
     gene            18884..19921
                     /gene="recA"
                     /locus_tag="PKU88_03570"
     CDS             18884..19921
                     /gene="recA"
                     /locus_tag="PKU88_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPX68394.1"
                     /translation="MNDKKKAIELALSQVEKQFGKGSIMKLGDATKMNVEAVSTGSIE
                     LDIALGIGGVPRGRIVEIFGPESSGKTTVALHVIAEAQKNGGEAAFIDAEHALDPLYA
                     KNLGVDIENLIVSQPDTGEQGLEIAETLVRSGAIDVVVVDSVAALVPKAEIDGEMGDA
                     HVGLQARLMSQALRKLAGAISKSRTTVIFINQLREKVGVMFGNPETTPGGRALKFYAS
                     VRLEVRKIENIKLGNDLTGSRTRVKVVKNKVAPPFKQAEFDIMYGQGISKEGSILDAA
                     VSNDLILKSGAWFSYGDIRIGQGRENAKQYLKDNPDITKELDKKLREMFNLGISKAKA
                     ADFDSEENDEF"
     gene            19937..20560
                     /locus_tag="PKU88_03575"
     CDS             19937..20560
                     /locus_tag="PKU88_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPX68395.1"
                     /translation="MRITGIEQQKKNTKRYNIYIDGEYKCTIEDNILQELNLSKGMEL
                     NEIEFNNTLEIIQYKGALRAALFMLARAPKTEMEIEKGLSDKQHAPKAIKKVLEYLKE
                     IGYINDESYAESYIRSIKATTGTSRRSIYQKLAAKGIDKEIIQQMLNKAEIDDYASAI
                     SAAKKKAASLKGNRREKKLKLLNYLYRKGFGIDDCKRAAEDILQEED"
     gene            20797..22326
                     /gene="rny"
                     /locus_tag="PKU88_03580"
     CDS             20797..22326
                     /gene="rny"
                     /locus_tag="PKU88_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPX68396.1"
                     /translation="MEYVIYIAIALITFIFGYLVRRYIAESKIKNAEEQALKIVSEAE
                     KQSETKKKEVVLEAKEEIHRLRAELDREIRDRRNEIQRAERRLLQREESLDKKVENLE
                     QRDEALAKKQKEIQKSLEEVQQLYKKQVEELERLSGLSSEDAKQLLLNNIEKQIRHEA
                     AMMIKDIETKAKEEAERKAKEVIASAIQKCSADHVAETTVSVVPLPNDEMKGRIIGRE
                     GRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREVARVALEKLIVDGRIHPARIEEM
                     VDKAKKEVDNYIKEQGEQATFDTGVHGLHPEIIKLLGRLRFRTSYGQNVLKHSIEVAH
                     LAASMAAELGADVKTAKRAGLLHDIGKAVDHEVEGPHVQIGADLAKKYKESADIVHAI
                     SAHHGDIEATTVEAVLVQAADAISAARPGARRETLESYIKRLEKIEEIANSFDGVEKS
                     FAIQAGREVRIMVKPEDVSDSEIVYIARDIVKKIEDELEYPGQIKVNVIRETRAIEYA
                     K"
     gene            22467..23246
                     /locus_tag="PKU88_03585"
     CDS             22467..23246
                     /locus_tag="PKU88_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPX68397.1"
                     /translation="MNILFIGDVVGRPGRNYLSTNLSRLVEENNTDFVIINGENSAGG
                     VGITRSTYDELLSMGADIITLGNHTWAKKEVLEFIEDAERLIRPANYPEDIPGKGYRI
                     LEKAGKRIAVINLCGRVYMDCIDCPFKAIDKILNELKNKADIIIVDFHGEATSEKLAM
                     GWYLDGRVQAVLGTHTHVQTSDERILPGGTAYITDVGMTGPIDSILGVDKEAVIRKFI
                     TGMPAKFEIAGGEVAVGAVCIQLDDNNKVTSIKRFTQNSKL"
     gene            23337..23597
                     /locus_tag="PKU88_03590"
     CDS             23337..23597
                     /locus_tag="PKU88_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPX68398.1"
                     /translation="MDVLKVSAKSNPNSVAGALSGVLREKGSAEIQTIGAGALNQAIK
                     AIAIARGFVAPGGIDLICIPAFTDIEIDGEERTALKLIIEPR"
     gene            23779..24603
                     /locus_tag="PKU88_03595"
     CDS             23779..24603
                     /locus_tag="PKU88_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPX68399.1"
                     /translation="MQKADLHIHTTASDGLMSPEKVVRWASSKRLTAIGITDHDTVKG
                     IGPAVKAASGYGVEIVPGIELSTLFEEEEIHVLGYFIDYKSQWLADTLKKIQNSRYER
                     AGKIVGKLNDMGIDITLEEVKGIADDGAMGRPHIARAMIDKGYISNIREAFAEYIGKD
                     RPAYVERYKLTCKEAIDMIEKLGGISVLAHPGLIKNKDNIGKIIDLGIDGIEVYHSKH
                     DDETVKNILAVAHSRNLLITGGSDCHGMKFNNEPILGNYSIDYKYVQMLKEALNKI"
     gene            24617..25819
                     /locus_tag="PKU88_03600"
     CDS             24617..25819
                     /locus_tag="PKU88_03600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPX68400.1"
                     /translation="MEIKLSDKVLNIAPSMTLAVNAKLKQMVDEGVEVFGFGVGEPDF
                     DTPEYIRNAAVEAMNKGFTRYTPAQGTIDLRKAICAKLKRENSLDYEPNQIIVSSGAK
                     HSLSNAFAAILNPGDEVIVPVPYWVSYTEIIKLNDGVPVLVQTKKENNFKMSGEELKN
                     AITAKTKAILINSPSNPTGAVYTEVELREIAEIAVQNNLFVVSDEIYERIIYDDNKHI
                     SIASFNSEIKDLTILINGMSKSYAMTGWRLGYAAGNEKLIKAMSSVQGHAVSHPSSIT
                     QYAAAAALNGPQDDLKNMVQEFNKRRNYMYGRINAMKGLRCIKPEGAFYVYVDISSYL
                     GRNLCGIKLDSCLDFAQVMLEKGHVAVVPGAAFGTEGFVRISYATSMEIIKNGLDAFE
                     SFIYKNIE"
     gene            25834..27267
                     /gene="purB"
                     /locus_tag="PKU88_03605"
     CDS             25834..27267
                     /gene="purB"
                     /locus_tag="PKU88_03605"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPX68401.1"
                     /translation="MDRKEYQNPLISRYASKEMSFIFSEDYKFSTWRKLWVALAESEM
                     ELGLNISPEQVEELREHIYDINYEDSEKREKETRHDVMSHVYAYGLQCPKAKPIIHLG
                     ATSAFVGDNTDVIQMSEGLRLIRTKLLNVIKNLAGFAEKYKNHPTLGFTHFQPAQLTT
                     VGKRSCLWMLDFLMDMEELDFQLGKLKLRGVKGTTGTQASFMALFNGDHEKIKELDAR
                     VTKKMGFEKSFEVTGQTYPRKLDSQVLNVLSQIAESAHKFSNDIRLLQSLKEIEEPFE
                     KGQIGSSAMAYKRNPMRCERIAALSRFVITNSLNPAITAAAQWFERTLDDSANKRLAI
                     PQGFLAVDSILNIMINVTAGLVVNEKMIKRHIDEELPFMATENILMEAVKAGGDRQEL
                     HEKIRQFSMEAGKRVKEEGLDNNLISLIEGDESFRLGKNKIQELLDAGNFIGRAPEQV
                     EEFLAEHVYPLLEKNKDKLNITADLKV"
     gene            27296..28687
                     /gene="asnS"
                     /locus_tag="PKU88_03610"
     CDS             27296..28687
                     /gene="asnS"
                     /locus_tag="PKU88_03610"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPX68402.1"
                     /translation="MSSIPIKQLYRSTEDYISKNIKVSGWVKTERTSKNFGFIELNDG
                     SFFKNLQIVFEEDLENFNDIAKLSVGSAIIAEGLLTESLGAKQPFELKAVRITIEGKS
                     TPDYPLQKKNHSFEFLRTIAHLRPRTNTFSAVFRVRSLVAYAIHRFFQEQGFVYVHAP
                     IITGSDAEGAGEMFRVTTLDIDNPPRTEKGEVDFSRDFFGKSANLTVSGQLNAETFCM
                     AFRNVYTFGPTFRAENSNTARHAAEFWMIEPEIAFAELSDNMELAENMVKYIINYVLE
                     NAPEEMEFFNRFIDNTLLDRLKNIVNSDFGIVTYTEAIDILKKSGVDFEYPVEWGIDL
                     QTEHERYITEQVFKKPVFVINYPKEIKAFYMRMNDDGKTVAAMDLLVPGVGEIIGGSQ
                     REERLEVLEKKMDEMGLNKEDYWWYLDIRKYGGVKHAGYGLGFERIIMYITGMGNIRD
                     VVPFPRTVKSAEF"
     gene            complement(28787..29029)
                     /locus_tag="PKU88_03615"
     CDS             complement(28787..29029)
                     /locus_tag="PKU88_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68403.1"
                     /translation="MSFKTNPLTVLLIPPNNSPVGSADREKGRGNRLRAGEAAGYDDG
                     GHAAGGNALYADECLRPIIKPPFKGIWNEMNPYSII"
     gene            29201..29276
                     /locus_tag="PKU88_03620"
     tRNA            29201..29276
                     /locus_tag="PKU88_03620"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29234..29236,aa:Trp,seq:cca)
     gene            complement(29524..30312)
                     /locus_tag="PKU88_03625"
     CDS             complement(29524..30312)
                     /locus_tag="PKU88_03625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPX68404.1"
                     /translation="MRKGLIAAVLLLFPAFSIDIYAEDTELIKELMACPEEETFSGLN
                     SDNYIEALKSMGYYKYDYKDELINLRNATLRFQSDCNLAADGIQGPQFKAAMIKRLSL
                     NGNYRPPDIIARPASRGYWIVINRTKRILTLYSGKTVIGKYPIAIGKATNKTPQGKFH
                     VCLKRKNPIWSPGDGSKPVAGGSPKNPLGTRWIGLSVGKGNLYGIHGNNSPYSIGRQV
                     SKGCIRMINSDVLSLYNIIPVGIPVWIGNDETLRKWGVTQKSFY"
     gene            complement(30576..30791)
                     /locus_tag="PKU88_03630"
     CDS             complement(30576..30791)
                     /locus_tag="PKU88_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPX68405.1"
                     /translation="MARNNQKVVPHAAAALDRFKYEIATEVGVTLKEGYNGDIAAKDA
                     GKIGGQMVRRLIETAERNMAGTTGTRF"
     gene            31015..31665
                     /locus_tag="PKU88_03635"
     CDS             31015..31665
                     /locus_tag="PKU88_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68406.1"
                     /translation="MKGVKPSVKALCSALAVCIIFSAGIAAFADNNAPSAVKSKHYLE
                     EAVEKMVQEGSLTREKADKILEYKQKRNEKLKKLPIEQKTQMKKQGGRGSLLKALVEE
                     GIITEEEAKSIREKLREMKEARVADGLQGLIDKGVLTKDDINNIRSYLLKVREERKSS
                     VEKLKSMTPEERKAYFKEHKNERKDIITKMVEDKVITKEQAEEIRKAIPELSKFRY"
     gene            complement(31794..33242)
                     /locus_tag="PKU88_03640"
     CDS             complement(31794..33242)
                     /locus_tag="PKU88_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX68407.1"
                     /translation="MKSNTIKWRIFKYNLTVIITLIVLVAIIFNAAIRIYIEKDIIGQ
                     LNKIASHAEETALKRGPEIFIFSKEIPPELPRIRIRTNDVFRFYFMLDRSLREPLSVL
                     NADYILMDNNKNIINSPVELYADPDEGLVGKISSEISKLKDLEKESYLNFHLSGTEYI
                     AVVKPVAQKNDFGLGWIIIYSSLQKVNQLQMAINIILFAILLLSAFIIVLFSSVIVKK
                     ISEPFSSLNQHIRSIAERNFGAKISTPVYDELSEFVTNINIMSERLETYDKAQKTFLQ
                     NVSHEFRTPLMSIQSYAEGIKYNVADSNEAAGIIIEESKRLTQLVENLLYLSRLDAME
                     ENYCLTTLDYNELLRSCAERMNMIACNNNIKIDISVPEENINIIADEEKLSRAINNIL
                     SNCIRYAKSSIEIKSEIIDEKTVRVTISDDGPGFESNELPNIFDRFFKGRKGNFGLGL
                     AISKCIIEKHKGKITAENSEAGAVFRIDLPMA"
     gene            complement(33247..33942)
                     /locus_tag="PKU88_03645"
     CDS             complement(33247..33942)
                     /locus_tag="PKU88_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68408.1"
                     /translation="MDKKLVYIADDEVNICNIIKSFLIKEGFEAETFNDGQSILDAFN
                     VKPADMLVIDIMMPGIDGYSLCRQVRQISSVPIIIVSAKDTEPDKITGLTLGCDDYLT
                     KPFSPMELIARIRSIFRRIELDKAQTGIGEVIEVSDIVIDHNIKKAKCHDKDIGFTGM
                     EFSLLYYLAANRNRAVSRSELLDKVWGFESEVETRATDDMVKRIRKKLADAGSRLKID
                     TVWGFGFRLTGKE"
     gene            34109..35836
                     /locus_tag="PKU88_03650"
     CDS             34109..35836
                     /locus_tag="PKU88_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPX68409.1"
                     /translation="MNSNTVPVRKHLIKLSRKKVLIAAAILLVAASVIGFNYYKSKNS
                     KNPAVQQATAIARMGDLSVVITGSGPISSSEKFTLTSNVNGTLTKIYFQDGDKVNAGD
                     LIFEIDDKDTQLQIKQIKNSIAQAELTRSSNISDLEAGTVTAPIDGEIHDMQVKEGDN
                     ISNNGTLFVITDKSELKLLVPFINTYRNRLSIGQKVAINAFDTERDELHRMEGTISGI
                     STPGYTTSSGAESYNVSIVIENNGSLKEGMIANASVDIDGTEVKSSGSNTLSYLKNMT
                     VRAPSGGTVSKLYAENGQNVKKGDILAELENKDLELAIKTNDLKLEDLNIQLQTAEEK
                     LLDHKIYAPFSGTLTLNDIEQGNSIKQGDILGSIANYDIMEFSINVDELDIAKIKEGQ
                     NVRVTIDALPDTTDKPLKGIVSKIAVEGTSSNGVSIYPVTIKIEKNEALKGSMSANGE
                     IIVNEKKDVLYVPIDAVVKMNGKDYVRVVPGAGGAGKAARNRNSDANGTEVKEDTKQN
                     TELQVELREVKTGISNAEYIEIVSGLKEREAVIVTSRSSGSNNQRGPNMMIMGTPPAG
                     GGNVRIRNN"
     gene            35850..36527
                     /locus_tag="PKU88_03655"
     CDS             35850..36527
                     /locus_tag="PKU88_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68410.1"
                     /translation="MIEICNMSKIYRIGNVAVKALDNISLSIKQHEFVSIVGPSGSGK
                     STLMNMIGCLDVPTSGEYCLDGMEVSGLTDDQLAYIRNHKIGFVFQGFNLIQKLNALE
                     NVELPLIYQGIPTMERYERSVEALKLVGLEDRIHHRPAELSGGQQQRVAIARALVGNP
                     QIILADEPTGNLDSKSGKDIINLLKELHLKGSTIVLITHDNDIAMMAERIVRIQDGQI
                     NDDRMVS"
     gene            36530..37708
                     /locus_tag="PKU88_03660"
     CDS             36530..37708
                     /locus_tag="PKU88_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68411.1"
                     /translation="MSFIQACKMAVKSIKSNKVRSFLTMLGVIIGVSSVIAAVGFAQG
                     STKSITDMISSMGTNLIQVNIMGRNSNRNVTYSQIKEFAEENSGEITAVAPQSNTSAT
                     VKYKTNSRSTNVLGTSPDYQIIRSVYAQQGRFLIQNDLENMQKVAVLGTAVVNDIFEG
                     TSPLGEKIKINGQLFTVVGILEEKDGGQDQGADDIVIIPATVAQRLFRSGIIRNFSIQ
                     ATSPDTVESAMEKLEEFLAKTYKDSDAFRVFNQAEMLENLDSMTGTMTAVLAGIAAIS
                     LIVGGIGIMNIMLVSVTERTKEIGIRKAIGARKKNILVQFLIEAIILTGFGGVLGVAV
                     GLSVIHFIIGGFGLVPEVYSIPWILISFGISLGVGVTFGLFPAYKAASLNPIEALRSE
                     "
     gene            37722..38873
                     /locus_tag="PKU88_03665"
     CDS             37722..38873
                     /locus_tag="PKU88_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPX68412.1"
                     /translation="MRRTFFILSAILCVSLLFNCIGAFADATEDGLLTLEEAKAMALK
                     NDVHYHHQDENIQAALEDYEKLSEDTSINSGRGSNIAQKEAARISKKIQLENAYSSVR
                     KVVLTKADLKRASYSDTANAFYAVIEAQNSLASALTDLELKRKELEKAEIKYMLNIVP
                     KKTLTQAEEACKNSEAAYNKAALELENCISKLCKIIGRKLDMSKVKLDTTLDIPDIKS
                     IDLDKIKEDNLKNNLSYFSAREQYKLAEYQLALTEEKYEDYYDDLKKGSSNVREEFDN
                     MLYDAQKKFEDAEYSWNEKLEDLEESLREQYESLEDLYENYLEQKEDIDDMKLTVKEN
                     KTKHAMSIITKAALDSSIASLERLERQLVSTIIKLNKQYISLVQCSIEE"
     regulatory      39043..39122
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            39199..40143
                     /locus_tag="PKU88_03670"
     CDS             39199..40143
                     /locus_tag="PKU88_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPX68413.1"
                     /translation="MNNLVSKITDLFIIKFIKNTKPEKKISDPATRRKYAYLEAYVSI
                     VGNLLLSAVKVALGLFLNSISLLADAAHTAADVLTSIVVLFGFKLSSSPADEKHPFGH
                     GRIELIATLVISAMLFLVGFEFAKSSYERLLSNTPVKGSYIIALIMIFGAIFKEWMAR
                     FSIDLGSRTSSSTLIADAWHHRTDGIASILVAAAIIASKFGYYKVDAVFGLMVSALII
                     YTGYSISKDSISQIIGETPAEEELNNIENIALTVSGVHSIHKVNVHSYGCHKEISLHV
                     QVDNDMSLVMAHDISEQVERIIEENMNCKAIVHIEPLE"
     gene            40985..41935
                     /locus_tag="PKU88_03675"
     CDS             40985..41935
                     /locus_tag="PKU88_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPX68414.1"
                     /translation="MTKLPDIAVEFLEYMETIRGKSKNTITAYSYDLALFFKYIKMKR
                     GYLVTDMEIDEIPIDDVDMYMINNISLADLYSFLSFAANQRDNKNYARARKVACIRSF
                     FKYLHKMKMIKENPSVELESPKINSRHPIYLTLEESKKLLSSIDGENKERDYAIITLF
                     LNCGLRLSELISIDINKISGDILTVIGKGSKERTVYLNKACIDAINNYLKVRPVDDVK
                     DKNALFLSERKRRISKRTVQYLVKKYIGISNLDMEKYSPHKLRHTAATLMYKYGKVDI
                     RSLQQILGHENISTTQIYTHLDDETLRKAINANPLSEEKK"
     gene            complement(41969..42226)
                     /locus_tag="PKU88_03680"
     CDS             complement(41969..42226)
                     /locus_tag="PKU88_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68415.1"
                     /translation="MEYKKECVLTDRECTDCGECDRCDIDPNKICDNCCTCIDKAADY
                     NSIEIDEIIEEEDAELDMEDHGKWKYERDYIVDYSEKEGND"
     gene            42401..43591
                     /locus_tag="PKU88_03685"
     CDS             42401..43591
                     /locus_tag="PKU88_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68416.1"
                     /translation="MINASANIFVFTAYVSLLIIEFVFLKILYIDFNTPWLITTLIYV
                     LFIIYQILVRVNEQVEKGRVKEILYCIIDSINGIPLVNKDNKIIIQWDVIFKKLIDKK
                     EYFLYDNIIKIKTKNIDRYVNQYPSLLFNSSMDMLERKNKIDTKSIIKGKESAPKELS
                     EELDLNEEMKKVLYVKYLSANKMTLANLDSSVYFDIVLNDEYEPPEFEKFGIKPDDRY
                     DGFSLVAYHKDKKYILVPRIMVYCKRIDKEKWLYRNIDRIIKCAENIAAGIQDYSIIV
                     DELSLKDVTTDYYEEETEVIPPFYFERLDNNILEFSGIGKGREVARAYCSLDTSHNSF
                     EINLQKYELIIDGVSEVVEEEKTIAIGNAYQPVVDIMEKMPIISNNIKLAILKKDIED
                     LVIK"
     gene            complement(43597..43887)
                     /locus_tag="PKU88_03690"
     CDS             complement(43597..43887)
                     /locus_tag="PKU88_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX68417.1"
                     /translation="MKRYVIRSKSRFIVSVGIILIFAMSSLFTLAVSARENKDVTMIP
                     EYVEVGDTLWNMSQKHKGDMDIRKYISRVMEINGLQNANIKPGELLYFPGFK"
     gene            44053..44673
                     /gene="lexA"
                     /locus_tag="PKU88_03695"
     CDS             44053..44673
                     /gene="lexA"
                     /locus_tag="PKU88_03695"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPX68418.1"
                     /translation="MNIDDLSKKQFEVLNFIKKEINKKGYPPSVREICDAVKLKSTST
                     VHGHLERLERKGYIRRDPTKPRAIEVLDSNSSIVTKKDMVELPVVGKVTAGLPILAEE
                     NIEDTFPVPIDFVGNSEAFMLIVKGDSMINAGIFDGDFVIVRKQSVARNGDIVVALLN
                     DEATVKTFYKEKDYFRLQPENPYLMPIIAKELSILGKVIGVFRSIK"
     gene            complement(44721..45242)
                     /locus_tag="PKU88_03700"
                     /pseudo
     CDS             complement(44721..45242)
                     /locus_tag="PKU88_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
     assembly_gap    45375..45474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45475..>46541)
                     /locus_tag="PKU88_03705"
     CDS             complement(<45475..>46541)
                     /locus_tag="PKU88_03705"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPX68419.1"
                     /translation="KVTRGDIEEYVEETGILMLEQEAAVYSAASGRVIQVSKKEGDPV
                     KAGEVIAKIDNSDIKLQIKALEAQKLGIEAKYAEAKTSADDETIRILNAQVRSAEAVY
                     EEAKRTMDSNKVLYEAGAISLGTYKNSLTNFAAAEAGLETAKSNLALAEKGISGNIKK
                     QYEAQLSEINARIEQLKLKSEEMIVTSPIEGLVITAEAEEGSIVQPGSKLFEIGGSEG
                     YYIESNVLIEDIAGVSVGSKVIIDDEEIGMQDIKGTVREIHPKAESIMSDLGIEQKRV
                     KVEISLDNEAAGLRPGYEMTVKIIVKSKKDTLLIDEKAVFSYKGKDHVFVNEGGTAKL
                     RAIEKGIESSEQVEVLRGLTE"
     assembly_gap    46542..46641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<46642..48879)
                     /locus_tag="PKU88_03710"
     CDS             complement(<46642..48879)
                     /locus_tag="PKU88_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPX68420.1"
                     /translation="MRKLDVRLLRMVRHSKGQFVSVTVIVAVALCIYILFSITSTNLR
                     NSVDYYYKITNISDLTIQLVKIPQGALENLQSIEGVRDVQGRVCVDVALEVEEEKERV
                     KIRLISIPDNGGSINKLYSEQKVSMKLEDNSSVILLEQFAKARNIKIGDVIYPRINGR
                     IHSLKVSGIASSSEFVYLMENEQSLMPAPEKFGVAYTSEAFAQSVFGYRGSYNEVLVT
                     TETDADIDDVIDRIEQKLDKYGVKKIIKLEDQLSNDVLEQELDGIEMLSSVLPVMFLT
                     VAAIIISIMLSRIVNNDRMAIGVLKALGYGNRIVLSHYIKYALAIGLAGAILGIWGGM
                     MLAGPISRYYTVFFNIPLAGVKIQYGYIFYGILLTCMFCTVSGLLGARRVIRIMPADS
                     MRPEAPKSGKRTFIEKVTFFWKHVPFSWKMVIRNILRNKKRFAFLVLGLAMAYGINTV
                     PLYMKDAMMSMFELQYGEYQKMDYTIQFNGPQNERIIVDLNNLIEVRRIEPRIEYPFE
                     LAKDWRKMTVSVIGVPRQTAFHKFIDMKDNVIILPESGIFITEAIAEKLNAKKGDTIT
                     IKSFIPGKDDIETKVTGIFKQYLGANAYMNIESMEELLLEEQLITGVSVAAQADLKDE
                     LKDVANISAVNSVDDMYRAFEEYMDLMLLAIGFYMLFGGVLGFALIYNSTIIGISERT
                     MEFATLRIMGFDRKDVYGMICRENFVMGGIAIIVGIPLGAGMIKGMVESFSSDIMTLP
                     YILKPE"
     gene            complement(48879..49589)
                     /locus_tag="PKU88_03715"
     CDS             complement(48879..49589)
                     /locus_tag="PKU88_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68421.1"
                     /translation="MQKKILMKIENSSKDYKMGEVTVNALCDASFEIYEGEFIAILGP
                     SGSGKSTLLNIIGGMDLPTQGKVFLRGDEINKYNDRKLTAYRRDKVGFVFQFYNLMAN
                     LTAKENVELATEMCKEALNIDEVMESVELGDRKDHFPSQLSGGEQQRVAIARAVAKNP
                     LMLLCDEPTGALDYKTGLLILSLLHKINRQLKKTVVIITHNMAIGDMADRIIKMRSGR
                     IVEIKENASPIPPERIEW"
     gene            complement(49540..50175)
                     /locus_tag="PKU88_03720"
     CDS             complement(49540..50175)
                     /locus_tag="PKU88_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX68422.1"
                     /translation="MNRQTEKKLNNLLDKSKELFLKYGYNAITVDRIANQAGISKMTI
                     YKYFNSKEDLFMEVIRKDIEYQANEIMKAISESGSSIERIEILYNYSTNMAKNYSLVL
                     IRDIMEKKSIVDKVTEIKKNFMLPIFEFIISEGIRNEEIRELDMGFISELLMNLPKAL
                     LNIDFPSDEGKMIKFYGNFIDFLKFGLFGGKEGVKSAEEDIDENRKFKQGL"
     gene            complement(50358..52283)
                     /locus_tag="PKU88_03725"
     CDS             complement(50358..52283)
                     /locus_tag="PKU88_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HPX68423.1"
                     /translation="MTSLSDLIKNDILYVINPKEYQREELINLLKDHPEIRFVSLAGA
                     DLTGNSTDERIPVRAFIEDIEEFLNGSVQTDGSSVVLPGIATLNDGKLDLVADTNSLW
                     FVDYNYENIDYETGRPVGTLRIPSFLMHGNKYVDSRSILIRGINRFRSEIMKILEKHP
                     SVCKEYGISCGDIDEILLTSATELEFWVMTPNDKADVEALTASQELQESYWKRTKGIV
                     RTSLEQSLILMDKYNLKPEMGHKEVGGIKAKLDETGNLTHIMEQLEIDWRYDTALQAA
                     DNEKWVRYIVKEVFRKNGLDVTFNAKPIEGVAGNGEHTHIGVAARLKNGKTVNLFTPS
                     DMKKDYLSIIGYGALMGLLKNYEAINPFISFTNDAFNRLKPGFEAPVCIVCSLGHNVE
                     TPSRNRTVLAGLVRNMSNPLATRFEVRSPNPKTNTYLALASIYQSMLDGIKAACESGK
                     TNSELEKEISKAPESEGFYLEKGRAYRSEEDVFEYYSQEERNRLFGVHPATVYENIKN
                     LAEYPEKLSVILEGNVLDERLVKSFTTAVMNRWVTEIINRLLPENMDTVRKCRAVHSK
                     QKAQDLDITYWAQVDHLRHYLMKDTYNSKSLFTRVREAAKEKEYETLSQLQKEMAEKM
                     SELKELYNDYKKNLIDI"
     gene            complement(52449..53744)
                     /locus_tag="PKU88_03730"
     CDS             complement(52449..53744)
                     /locus_tag="PKU88_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HPX68424.1"
                     /translation="MHYKTTDYLINKLNISKKIIDICVSTEEEVSVQFKKIDEVSEFN
                     QYKVLAAMQRNRISEMHFNCSTGYGYGDVGREALEKVYAEVFACEDALVRPQIVSGTH
                     ALALCLFGVLRPGDELISVTGKPYDTLENIIGTKDKRPAGCLTELGVVYKQIELTEAG
                     KINYNEITKKISEKTKMILIQRSLGYSWRPSLTMEDIEYTIKFIKIMNPNIICLVDNC
                     YGEFLDFKEPTEAGADIIAGSLIKNPGGSIAPTGGYVCGRADLVEMAASRLVAPGIGK
                     ECGATLGINRLFFQGLFIAPHIVSEAVKGAVFCSRLFSKLGFEVNPDYNTKRSDIIQA
                     IKFNNRDALIAFCQGIQKGSPVDSFVDPEPWDMPGYNDQVIMAAGGFVQGASIELSAD
                     APMRPPYIAYLQGGVTFEHVKIGLIYALQNLAGKGCISI"
     gene            complement(54123..54380)
                     /gene="hfq"
                     /locus_tag="PKU88_03735"
     CDS             complement(54123..54380)
                     /gene="hfq"
                     /locus_tag="PKU88_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPX68425.1"
                     /translation="MNKVNINLQDLFLNQVRKEHITITIYLVNGFQLRGNVRGFDNYT
                     IVLDNEGKQQLVYKHAISTIIPVKPINFAFSDNKPTENTVK"
     gene            complement(54461..55402)
                     /gene="miaA"
                     /locus_tag="PKU88_03740"
     CDS             complement(54461..55402)
                     /gene="miaA"
                     /locus_tag="PKU88_03740"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPX68426.1"
                     /translation="MPKICILAGPTAVGKTEISLALARSLCGEIISADSAQVYKYMDI
                     GTAKLKEEEMQGIKHYMIDEVTPDMDFSVAQFREKAELYIKDINDRSKLPIITGGTGL
                     YINSLLNNLDFTDSISDEEYRREMQETAQVKGTDYVHAMLEAVDPASYKKLHPNDLRR
                     VIRALEVYKHTGRPISYFQEKSRKQPPRYDFAYITLTMDRRKLYERIDQRVDRMMASG
                     LVEEVEGLIKRGYGRELNSMQALGYKEIADFLHGLITKDEAVRIMKRDTRHYAKRQLT
                     WFRGDKRVNWVDVDNFFRKEVIVENIIRFIAGKIPLI"
     gene            complement(55423..57189)
                     /gene="mutL"
                     /locus_tag="PKU88_03745"
     CDS             complement(55423..57189)
                     /gene="mutL"
                     /locus_tag="PKU88_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPX68427.1"
                     /translation="MGKIHILDEATINKIAAGEVIERPASIVKELVENSIDAGAAAVS
                     IEIQDGGKSFINVSDNGCGIMKEDTEQVFFRHATSKIKDSADIQGISTLGFRGEAMAS
                     IAAVSEVELLTKAEEDAAGSHVIIKGGKLVQSKSIGYPTGTSVTVRNLFYNTPARLKF
                     LKSDTSEQGAIVDIIEKLAMTNTGISMKLTVNNKVVLHTPGNGDLLSVILCIYGKSVA
                     GAMLPLEYSNDIISIEGYIGKPEIARGNSTYMTFSVNKRYVKNRMMSEAVKQAYKTLL
                     MNNRYPFSIININIKSEMTDVNVHPTKQEVKFSDERAIFNTLYLAVKSCLNNNRLVFE
                     NLEDVTEPQTAPSAAFESRNSYEPEKVSFDQLSFGREAAAPRADSYKWQYDYDRKTKV
                     QQKQFDNEIRVIGQLFGTYILGQKDDDFYIIDQHAAHERIMYEYIKDKYNEKNISVQE
                     LMMPIVIELSPAEKVLYAENSLIFQNLGFEIEWFGENTLAIRAVPILMGEPCTGEFFS
                     NILDSLKESAPNASSPLEKIIISMACKNSIKAGDTITPEEISVLLERLMKTCQPYTCP
                     HGRPTIITMSRYEMEKKFKRII"
     gene            complement(57201..59801)
                     /gene="mutS"
                     /locus_tag="PKU88_03750"
     CDS             complement(57201..59801)
                     /gene="mutS"
                     /locus_tag="PKU88_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPX68428.1"
                     /translation="MAQFTPMMKQYFEIKNQHKDKILFFRLGDFYEMFFEDAITASKE
                     LEITLTGRDCGQEERAPMCGIPYHAAESYIARLIEKNYKVAICEQVEDPALAKGIVRR
                     EVIKIITPGTVIESSMLDERVNNYLLSLYADGRQAALSYTDLSTGEFYCTYADISDAA
                     VVRNEINRINPAEIISNTDFQDIKAAYSAYNILDKRYFDHEACERRLKRQFGVSSLEG
                     LGIDNETLLRSTGALLLYLDDIQKVSLDNINYIKTYSISNFMVLDQSTRRNLELSETI
                     RGKSRKGALLSVIDRTSTAMGGRKLKSWLEKPLLSTVEINGRLDAVAELYDDFLKREE
                     IKEYLKSMYDVERLATRIALGSANGKDLIAFKNSIRNLPYIKSLLSDCTSILLKDIYG
                     KLDVLEDLFGIIDASINEDPPFVLREGNLIKDGYLPELDEIRSASVNGKQWIAELEQA
                     EKEKTGIKSLKIGFNKVFGYYIDITKSNLPSVPEYFIRKQTLSNSERYITPELKRLED
                     LVLGSEEKLIELEYQTFIQIRNEIAGHIKRIQQTASCLSDLDCLCSLAEVSQKYNYVR
                     PEVNNEDRIIIKNGRHPVVEAQDNELKFVPNDTQLDNDENRMLIITGPNMAGKSTYMR
                     QVALITLMAQIGCFVPASKAEIGVVDRIFTRVGASDDLASGQSTFMVEMSELANIINN
                     ATSKSLIILDEIGRGTSTFDGLSIAWSVVEYIADKARLGAKTLFATHYHELTELEDKV
                     EGVKNYCILVEEKGKDIVFLRKIARGGAGGSYGIQVARLAGIPEAVLERSSEIISILQ
                     KSEKNSHTDVYVRRRRRNPVINDHEPNLFNFASFSLAEELKSVDLVNMTPVQALNKLQ
                     ELQDKAKDMI"
     gene            complement(59801..61246)
                     /gene="miaB"
                     /locus_tag="PKU88_03755"
     CDS             complement(59801..61246)
                     /gene="miaB"
                     /locus_tag="PKU88_03755"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPX68429.1"
                     /translation="MSERKAPEKVNNAEIERQKQIALRVRENNDIEKLKTGKQKRMHV
                     ITFGCQQNNSDSEKIMGMLAEMGYGKTDIKEEADVIIINTCCVRENAELKLYGNIGAL
                     KKLKEDNPSLIIGVCGCMMQQEHAVEMIRKKYRHVDIIFGTHNLYKLPELLERSINEK
                     YTLIDIMNTEGMIIEDIPVLREEKFKAWVTIMYGCNNFCSYCIVPHVRGRERSRKPGD
                     IIREIESLAKDGCKEVTLLGQNVNSYGKNLNMEMDFADIVRMVNEIEGIERIRFMTSH
                     PKDISDKLIYTMRDCKKVCEQLHLPFQAGSDKILKRMNRKYTKEEYLNKVFKAKQEIP
                     GVALSTDVIVGFPGESDEDFEETLDVLRRVEFDQVFMFIYSRRIGTSAAEMEDTSGYE
                     DKHRNFEKLVELQNGISKKLNDEYMDKIVEVLAEGSSKNDPSKFTGRTRTGKVVNFTG
                     TDGIIGKLVNVRINEIHSWFLNGQMVDEVKK"
BASE COUNT        18836 a        12273 c        12767 g        17188 t
ORIGIN      
        1 gatgtattag aaaacgattg aaaaaatacc gtatattata tataatgaga tgtacatttg
       61 aaaaaaacta aaaaacccgg aggtaaatta atgtatctga aagagaccct taaaaatgga
      121 ataagaatag ttgctgagga aataccatat gtcaattccg tttccatcgg tttatggttt
      181 aaggtgggat cacgatatga gagcattgag aataacggta tttcccattt tattgagcat
      241 atgcttttta aaggcacaaa aaacagatcg gcaaaggaaa tagcaaacag catagataaa
      301 ctcggaggcc agcttaatgc gtttacatca aaggaatcca catgcttata tgcgaaagtg
      361 ctggatactc atttcgatat agctttggat gttcttgcgg atatgttttt caattccaac
      421 tttgactgcg atgaaatcga taaggagaga ggagtagtaa ttgaagaaat aaacatgtat
      481 gaggattccc cggaggatct tgtccatgac ctcttcagcc agtcggtatg gtcgggaaac
      541 cctttgggaa tgtccattct cggaactgag gaatcccttg aaaatcttaa cagagatata
      601 atagtccgat attttaaaga aaactattcc cctaagaata ttgtcatttc cgttgtgggt
      661 aatttcaagc ataaggatgc ttttgacaaa ataaaggcat attttgagaa agccgaactg
      721 attaataggt ccgctaccga tattgcagtg ccccgctttg agcccgagta tacacttaag
      781 agcaaggata ccgagcaggt acatctttgt atgggcttca acggtgttga tattaagaac
      841 aaggcatttt atcccatgct ggttttaaat aatgttttcg gcggagcgat gagttcaagg
      901 ctcttccaaa aaatcaggga ggacaggggg cttgcatatt cagttttctc atatccttct
      961 tcctttgagg actgcggact gttctccatt tatgccggtt caaagcctga taatcttaaa
     1021 agcgttgcga agcttataat ggaagaaata aacatgatca aggacaaggg cattacggag
     1081 gaagagcttt atgattcaaa agaacagctt aaaggcagtt atatacttgg ccttgaaagc
     1141 accagcggaa ggatgatttc cataggcaaa tcagagcttt tgcttgacgt aatatattct
     1201 cctgcagaga tattggcaaa aatagacaag gttgatatgg acaacgtgaa cagtaccata
     1261 aagcacatat ttgacacgga cagcatgggg gctgctgtaa taggaaacat gaaaaacggc
     1321 actgatataa ggaatctttt caaataggtg aaggatgtga agcaaagtga aggatgtaac
     1381 agtaaaatac atcaggacaa gcccggatga aaacccggaa ccaaagatac ccgcatatac
     1441 aacggaaggc tctgccggta tggatctgag cgcgtgcatt tccgaggata tcatcataaa
     1501 acccggggaa attgcaatgg tgaaaacagg aatagcaata cagattcctg acagcagcat
     1561 tggaggcttt gtatttccga gaagcggcct ttccagcaaa cacgggattt cccttgccaa
     1621 ctgtgtcggc gtaattgatt ccgattatac cggcgagata atatgccccg taatcaatca
     1681 cggaagcagt gactatacga taaaaaacgg agacaggata gcacagatag tatttatgcc
     1741 tgtagtgaat gtacggcttc aaaaggcgga caagctggaa gccacgggaa gaggagcggg
     1801 aggcttcggc tcaacaggca agtaaattaa acaggaatat tcgtccgctt cattaatata
     1861 attaactata gaattattga tggggtggta ctatgagatt cagcaagctg ggcgggaagg
     1921 agattgtttc ccttcctgac tgtgaaaggc tgggctattt gggcgactgt gaccttatga
     1981 ttgacgggga aaccggcagg ataaaatctc ttatagttcc tgaaaacaaa gggacattat
     2041 cgttttttat agacagaaga tatatagaga tcccatggga aagaataaaa aaaataggca
     2101 acgatatgat aataatagat tttgcggata ttttaaaata gaaataagag aaatggtagg
     2161 ggacatgggg acgtttctcc tgtcccgctt tcagcgggac aggagaaacg tccccatgtc
     2221 cccttgtaag acacgcgaaa cgtccccgtg tcttgcccat gtccttgtgt tttatgcgct
     2281 ttggataatc accaactgca tataaatctc atattataaa ttagttatat gtttggagga
     2341 atctgtatga aaagctcaag ataccttatt atcggcggtg atgacaggct tgtcgagctt
     2401 gcaaagcttt ttgaagggga agggactgac atcgccactt atgggatgga catgactgta
     2461 ataaagaatg taccaaatta tttatccttt gatgaagcgc tggaaaactc ggatatagtt
     2521 atcgggccga taccctttgc caaggacgtg cacaaaatta attcaaagta ttccagtgtt
     2581 gatattgaag ctgaagagct gttcagtaag ctgggaaagg ataagaagct gatattgggt
     2641 gcaataaaca attattcaaa ggggcttgct gaaaaatacg gcatcgaata ctgggactac
     2701 cataatgatg aaggatatca gatactaaac gccatcccga cggcagaagg cgctctgtca
     2761 atactggtta acgagacgaa aacgacattg ttcggaagca gaattctcat actgggctat
     2821 ggccgtatag gcaagctgct ttccgaatat ctcaaagcat taggtgcatc gttatatgta
     2881 gaagcgagaa aggattctga ccttagctgg ataactgcag agaggatgaa agccataccc
     2941 cttaaagagc ttcctcaata tctgggtgaa atggaaataa taatcaatac cgtacctgca
     3001 atgctgctgg acagcaatat gcttgatttg gtgaagcagg aagctctcat acttgatctg
     3061 gcttcagcgc ccggaggaac tgattttgac tgcgcctcaa aaaaaggcat aaaagcaata
     3121 catgcccagg gaatcccggg taaagccgca tgtaaatcag cagccgaata tatttacagc
     3181 acaataaaaa aatatttatg tgctgatcgg cagaaaggag tttaataatg aaagttgaag
     3241 gcttgaatat aggattggca gttaccggat ccttttgcac ctttgataaa ataattcccg
     3301 aggcagaaaa gctaattaag caaaaagcca atgtataccc gatattttca acaaatgctg
     3361 cttcaatgga caccagattc ggaaaagcgg aggactggct taggcgattt gaggaaatta
     3421 ccggcaacag tgtgataaaa accatagaag atgctgaacc cataggcccc gggaacatgt
     3481 tggatgtttt agtaatagca ccctgtacag ggaatggttt tacaatttca aaactacgtt
     3541 gacatcgccc ttatatgctt ctatcctttt tatcgggagt tcaggcagct gcctgtgcat
     3601 tgacggctcc ttttttcccg taatcggctc aattgcttca agaaccgctt ttgtaagttt
     3661 ttcggcattc acataatttg aacaattgcg gcatctcaga taaatatact ttttgccctt
     3721 tgaggaagca tgaaagctgg taagcggcat tccgcacttc atgcaaaagg cttttaattg
     3781 catccccgaa taatcggatg ctttccgtga agttctcttt ctcgttccgg tacttgcttt
     3841 ttgagcttta ttccacagct catcatcaat tattgcatca tgggttccct tgacgctgta
     3901 ctggttttcc ttgcctatca agtgcctgtt gataccgatg tagaccgtat tgtgcaggat
     3961 atatgatatg ctgctgcctc tgaattcaca gcctctctta ctctttaacc ccatgctgtt
     4021 cagatatcct gctatggcgg aacagttcat gccgccttca gcatataacc tgaatatcaa
     4081 ccggactatt cctgcttcct cttcatttac aacgagtttt ttgtcaatat tgtcataacc
     4141 cagtaccgta gatgatttcc ctccctttct tgcaaactcc ttaagcccca gctttacatt
     4201 ctcaacaata gtattccgct caaattccgc aactgagcca agaagctgca atgtcatgcg
     4261 gccaatcggt gttgatgtgt caaatttttc tgagcagctg gcaaaacata caccattatt
     4321 ttcaaaaata tctatgagat ataacaggtc tttaagattt ctggacagcc tggagatttt
     4381 ccagaccagc acaatatcaa aaaaacggct tttacaatct tcaataagcc gcttcaactc
     4441 gtgcctgtcc ttcatactct tgccggaaac acccagatcc atataataat catatatcga
     4501 tataccgaaa gcaatgcaat attgcttaag ggtttcagcc tgtgcatctg cggattggcc
     4561 atccagagcc tgttcttcag tactgaccct gatataaagc gcagccctca aactttcctt
     4621 gtctgccatt tgctcacctt acatttttct gcttagtaaa ttatattaac actcattgaa
     4681 taataagcac ctaaaaaatt gaaaaaactg cggcataaaa ttccgcactt taagcatgtt
     4741 gtacccggag ggcagaattg ctataattta gtagaaagag ttatattatt acttataaaa
     4801 ggagaagccc tatgagtatt atcgatacct ttctgttaag atacgccaag gaatatgatt
     4861 tttacaacga attggcccat caggtggcga tgatttgtga aaccataata cacagaagcg
     4921 gaatcagagc tattgtgacc tatcgcgcga aaaagcccga aagcctcaag gataagctga
     4981 tgaaaaggaa tgccatcaaa aaatatcaga gcatcgaaca gatataccgg gatatagtgg
     5041 atctttccgg agtgagaata gcaatttatt ttcccggcga cagggaggaa atagggaggc
     5101 ttattgaaaa cgaattcatt accagaaaaa cgaaaaaatt cccgaattcc gagcagaagc
     5161 agcatcagga taatccgttc aaaaggcgtt tcacgggcta tgatgccgtt cattacagag
     5221 taaagataaa agaggaaaag ctggaggaga acaataagag attcgcacac gcccaggtag
     5281 agatacagat agcatcagcc cttatgcacg catgggcgga agtggaacat gaccttgcct
     5341 acaagcctca gataggaaag ctttccgagg aggaattcaa catattggat gagcttaacg
     5401 gccttgtact ttcgggggat ataactttgg aaaggcttca aaagtcattc aaacaaagga
     5461 tagcctcaat ggagcagcaa ttcaacaatc attacgagct tgccgccctt ttgagggaaa
     5521 agatatccgg cagctttccg gaaggctgct tcatcggcag agttgatatt ctgctgaaat
     5581 ttcttcaaaa gataggcatg gataatccga aggaaataca tatgtacctg gattcttttg
     5641 acaaaatgaa ggaatccgta ggatccaaaa ccatagtgga ttgttttgcg gatcttatat
     5701 tgaatcatta ccccgattac tatagacagt atgttgaagc aaaatttgaa tcaagcttca
     5761 ataatccgta catatccatc caaaaacaga tgcttctcgg taaagagaat gagcagctca
     5821 attccttcgt tgaaaaatgg atactttttg aaaatgctat aaaggctttg gaacaaaaaa
     5881 acataacgca gagccgaagt atttccgata ttataagaag gcacctgact gctgatgaaa
     5941 ttatacttga tactgtcaag aatctggaga aaaccaggaa tcatataata tacagcagtg
     6001 atataccgga taatttacaa ttaagaaatg ctgcggactc aataacatta atactgaaca
     6061 agcttgcgga actggcttcg gatgagaata aaacagttat ttgcaaggct ttggataaaa
     6121 taatcaaaga gttttagctg caaacttatt ccctacaggc aatactttat caaaactggc
     6181 aaatgcgata actgatacgc ctgtgttaat ggcggcaaag gctcatgtaa gaaacggcaa
     6241 gccaattgta gtagggattt caacaaacga cgcactgggt ttgaatgcga agaacctggc
     6301 tgtactgctg aactcaaagt attactactt tatccccttc tggcaggata atcctgctgc
     6361 aaaaagaaat tctatgattg ccgatatgga gcttttgata ccaactgttg aaatggcatt
     6421 ggaagggaag cagcttcagc caatgataat tgagaaatct aaatagagag gaacaatcac
     6481 atgaagataa ttgtacaaaa atttggcgga acctcagtat caactcagga aagaagagaa
     6541 ctggtggttg acaaaataga aagtgcagtg aaaaaagggt ttttgcctat tgttgttgtt
     6601 tctgcaatag gaagaaaagg tgacccctat gccacagata cccttataaa ccttgcagct
     6661 tccgcaggag tcaaaaacaa tcccagagag ctggatatga ttatgtactg cggtgagata
     6721 atatcctgtg ttgtactgtc taatacaatt aaaaaaagag gctatgacaa tatagtactg
     6781 acgggcgggc aatccggaat aataactgat gagaaattcg gtgacgccga ggtcttaaga
     6841 gtggagcccc attatataaa agaatgcatt gaaaaggggc tcatacctgt tattgcaggc
     6901 tttcagggca tatcggagaa gggtgaaata acaactctcg gaagaggcgg aagcgatgtc
     6961 acaggtgcga taatcggtga agcccttaat gctgaggcgg ttgaaatata tacggatgtt
     7021 gaaggcgtca tgacggcgga ccccaaaatt gttccggatg ctgcggtaat tggtaccata
     7081 tattacaacg aagtttttca aatggcggaa tacggtgcaa gagtaattca tcccagagcg
     7141 gtagaggttg caatgagaag caatctgccg atatttatca agaacacaat gtcagactcg
     7201 tcaggcacat tgattaccaa ttgtgataaa aaccgaagat acagatatga taagaatgat
     7261 aaattgctga cggctgtggc ccatgtaggc ggaagaacga gagtaagaat actgtttgac
     7321 ggttcggcaa aggaatatga gaatgacagc catctgttca gtacaatcgc ttcggaggga
     7381 atcagcatcg atatgattaa tgttaatcct gattttaaat tatttattat tgttgatgat
     7441 gatgttgaaa agcttggaac agtgctgaac cgatgcggat ataactatga gctgaaaaag
     7501 gattgcagca aggtgacgat aatcggaaac aaaatgcgcg gggttccggg tgtaatggca
     7561 aaagcaataa gggccctggc aaaacacaat attgagatcc tgcagacatc cgactcccat
     7621 acgactattt cctgcctgat agaaagcata aatacaaata ctgccgtaaa tgcactgcat
     7681 catgagtttg agctgggaaa accggcaatt cagaacctgt gaggcatctt tttcccgtta
     7741 taaatgaatt cctctgtttc caaaggatat cggaggaatt cttttttatg ccggtttgtg
     7801 ctaatatatt aatgtattga aataatcggg aatttctaaa aaaagtgaaa caaaacagga
     7861 aaatattagt ctaataatag gatgcactga caagcgcagt tttttatgga agagtgggta
     7921 atatggataa aatcatacag ctcaaaaata tcagaaaagt atacagaatg ggaaacgaaa
     7981 aggtcatagc cctggacaac ataagctttg aaattgaaga aaaagaaatc tgctgcctgt
     8041 taggcacatc cggctcgggc aaatcgaccc ttctgaatct tatggcaggc cttgaaaaac
     8101 caacaaaggg agaagtgaaa ataaagaact ataaccttga aagcctgaat gaaaaacaac
     8161 tggcaaagct tcggcaaaaa tacataggct ttgttttcca atcctataat ctgcttgaac
     8221 actttactgc tttggaaaac gttactttgc cgctgatatt caaaggagtg cccaagcctg
     8281 taagagagaa aaaggctaag gccttgctga aggctgtcgg cctgggtgac cggatgaatc
     8341 acaagccctc acaaatgagc ggtgggcagc agcagcgtgt aagtatcgca agagccttta
     8401 tctcaaatcc tgagatagtc tatgctgatg agcctacggg taacctggat accagaacct
     8461 ccgccgaaac aatggacctg ataaccggaa tggccgagga atacaagcaa acacttatta
     8521 tcgtgactca tgacttggaa atagccgggt atgcaaaccg aatcatatat ataaaggacg
     8581 gaaacgtaga aaaaatagat atgctgagtg aaaaggcggg aaggggttaa tatgaaaagg
     8641 aagttattta ttgttttttt agctgccatt atagcatttg cgggtctgcc tgctataaat
     8701 gtatcggcag gtatttatga tgatattgaa gtatcatacg atgttaatga atcaaggata
     8761 ttggacggtg atagttttac actatatttt actattgatg aggtgcgtgc aggtgaaata
     8821 agtgatgttg aaattaatat tgaaaccgga agtaatttca aagtactggg agggaatctt
     8881 aatattcccg ctgctgttct ggataaacaa tacaatgtta atttgcagta tcttggagga
     8941 agcaacagcc tgccgataaa aataaaatac gacaaaaatt ctgtatatga gggggagttt
     9001 atctataatg tagaaattgc agagatagat gaagagagtg tcaatccgtc ctcaagcagc
     9061 tccgacaacg agcccaagct gattgtagaa agctcttcca ttcctgtggc tgctgccggt
     9121 gaaaccatga aaatacccat tacagtaaca aatgaaagcc ggtattctgc taaagatgtc
     9181 actgttaaac tttcactcgc aaacaatgac aataatccct ttgaaataag cagtctcaac
     9241 ctggtacaat ctatcggcag tttaaatgca aagaaaagcg acgggttgat cttcagcctc
     9301 aaagtaaacc catacgcaga agaaaagacc tatgaattag atgtacaatt tagttactat
     9361 aactcaaaga acaacttatt taccagcaat gagaaaatat atgtaaaggt taccaacagc
     9421 aagaatgagc cggtgcttat gatccaggat ataaagcaca gcacatatcc tgtaaatgcc
     9481 ggggcaaaat tccagataag ctttaacgtg gccaatgttg ggggtattga tgccaggggt
     9541 gccaaggttt ccatcgaagg cctgaacaaa gacggattta ccctgtacag cggtgttaac
     9601 agctggtatt tcgacaatat agaaggcaac gggaaaaaga atattactgt ggatcttgta
     9661 gctgctgact ctatggaaag cggaaattac ggcctggaaa tcaagatgga gtataaggat
     9721 caaaggaaca acagctatac tgccagctca agcttttttg tcactgtcaa aggagaagcc
     9781 aaaagctcaa atgtgacaat aaaaaatatc tccgtattgc cgcaggaagt cagggcaaac
     9841 gagaacttta cgttaagctt tgatgttctt aatgaaggca aatccgatgc aaggaatgtc
     9901 aaattgacag ttaagggaga cgagggcata atatccaaat ctccggatgt aagaatcatt
     9961 gagaacctgt ctgcgggaga acagcagaag ctggaatacg tgctctatgc caataatgaa
    10021 acgaaaactc agaattataa cataatgata aaccttgaat atgaagatgc ggataaggag
    10081 aattcaaagc attccgttaa tcagtatgcg ggagtatatg ttaacggggg aaacagcaag
    10141 attactccca aaataataat aagcaactat aattttgaac cgaagctggt cagggcaggc
    10201 gaggaattca gcatggatct gacctttatg aatacaagca gcacaaagga agtaaagaat
    10261 ataaagatat atctgacggg aatagattcg gataaagagg gaaatgtggt attcacaccg
    10321 gtgggaagca gcaatacatt tttcgttgat gccataaagc ccaagggcac cgctgtaaga
    10381 agcattacaa tgtacacaat tcccgatgcc cagcccaagt actataatat aacggcaaat
    10441 atcgaatacc aggatgatga aggtaccgag tacaaagctg aggagctgat cggaattcct
    10501 gtaatacagc aatccaagct ggatgcatcg gaaattgggt tcccgccgga aacattcatc
    10561 ggccagccca ttccggtaag tcttcaatat tataataaag gaaagacaag gctttccaac
    10621 ctgatgataa agattgaggg tgatttccgg atagaaaacg gagaagcctt cataggaaat
    10681 tttgaatcgg gctccagtga ctactatgag gcaatggtta cgccaatgaa acccgggcct
    10741 gccaaaggaa aagtagtttt cagctatgag gatccatcag gtgatgagtt cacctatgag
    10801 agggaatttt ccataaatgc aattgaaatg cctcccatgg agatgcccgg cggagagatg
    10861 cccatgcctc ctgtgagcac taaggataaa ataatgaaat atacaattaa gaattggttt
    10921 ttctgggtgt gtatcgcagt aatagctgca gggattttta ccgccaggag gctgattagg
    10981 aacaagagac ggaaggatat ggatctggat gaataggata gatatagtaa ggatagtcct
    11041 taaaaacctt ctcagaagaa aaatgagaac aatgcttact gtaatgggcg tagttatagg
    11101 cactgcttca attgtaataa tggtttcaat agggatagga atgaatgagg gcttcaagag
    11161 tgaaatatcc agaatgggaa gccttaattc aataaatgta aggtcccctg aaacaggtat
    11221 gtactatatt tatgatgaaa cagaggcggg aaggacaaat tctacggaaa tgcgggattt
    11281 ggatgatgct gcagtagcag atttttcaag aattgccggt gttgaatcgg taattccctt
    11341 cattgagact catggaaaaa taaagcttgg caaatatatt ggctatgtcc agcttcaggg
    11401 aatcaatccc gagcatatgg ataaacttgg tttgaaggtc acggaaggaa gactgctggg
    11461 tgccggggat aaaatgaata cagtatttgg tttcggagta aaagataact tatataaccc
    11521 taaggggtcg caaaggcgaa gatttggcag aaacacaccg gaggtagacc tcttgggtga
    11581 aagaatcact ctttcattgg atatgtctta tggagaggaa ttcatccaga gagatacttc
    11641 tgttcagaaa aagcccaaaa catataaaat aaatggagtc ggtttgcttg aagagggaag
    11701 ctatgaatac tacagcagca tttttatgga tattaccgag cttaaaaaaa tagtaaagga
    11761 tttcaacaag ggtaagagta agaatgataa ttcaggagat taccgggaca ttaagggtta
    11821 ttcaagaata atagtgaaag tgaaagatat aaatgatgtg caaagaataa aaaatcagat
    11881 aaaggatttg ggattttttg cagacagcct gaccgatatt ctagaatcaa tgcaaaaaac
    11941 cacatccagc ataagaatcg ttctcggtgc aatcggtgca gtatcccttc tggtcgcatc
    12001 cattggaatt accaatacaa tggtaatgtc tatctatgaa cgcacaagag agataggtat
    12061 catgaaggta atcggctctt ctctgggaga tataaaaagg atgtttcttt ttgaagcatc
    12121 aatgataggg cttttaggag gactaatcgg attgctgctg agctatggag tatcctacgg
    12181 gcttaatacg gcaggctttg cattgattgg cttcttcggc ccgtccggag gaggaagcaa
    12241 accctcaatt atacctttat ggcttgcagt tagcgcaatg ctgtttacga caattatcgg
    12301 acttgtgtcg ggattttatc ctgcgcaccg tgcaatgaat ctcagcgctc ttgaagcaat
    12361 aagaaaagaa tagccatgca gcccttcaaa aaatacgaca ttgtcgtatt ttttatttta
    12421 ttaaggtaaa aatattattt aaatgtatag ctgaatgtac aatggctaac gaaaaaaatc
    12481 gggagagtga tttccttgac agaagcagat ggagcagata aagaaaaaga ggctaagaat
    12541 aatgccaatg aaaatattaa agaactgggc gaagtcagag tgccgcaatt caggagcaat
    12601 atacactgca ttacaataat aggccagatt gaagggcata tggtgcttcc gccgcaaaac
    12661 aaatcaacaa aatatgaaca tataataccg cagattgtag ccgtagaaga gaaccctgag
    12721 attgaggggc tgctgttaat tctaaataca gtaggagggg atgtcgaggc gggacttgca
    12781 ttgtcagagc ttatagtaag catgaaaaaa ccgtcagttt ctcttgtgct tgggggaggc
    12841 cacagcatcg gagttcccct ggctgtttct gcaacacatt cctacattgc gtccacagcg
    12901 accatgacaa tacatcccat aagaatgaac ggcctggtta tcggcgtacc gcagaccttt
    12961 gactatttta ataaaatgca ggagcgaatt atcagattta tagtggagca cagcaatata
    13021 gacgttgata caataaagag gctgatgttt acaacaagcg agctggccaa cgatataggg
    13081 acagtgcttg taggagccga agcagttaaa tacggcctga taaaggaaat aggagggctg
    13141 catcaggcac ttgcaaaact gcagaacctg atagcggctt ctaaagcagc ggaaaatcaa
    13201 aggagtaatt gaatacaaag gattagagaa cattgtaaca tttgtcaagg atctgccgga
    13261 aacgtcaggt ccttaagttt tgcaataata cataagcaag gttttgttgc aaccgattcc
    13321 aaataaagct ataataacat taagtttaat atacttttta agaggtgata acttgtcagg
    13381 gaagagaaaa gagaacgatt taaactatga aatatctgga gtgtctctga tattagccag
    13441 tatatacata tatataagcc ttttcagcag caattcagga attgtaggag aaaaagtcag
    13501 gttatttttt ctgggaacct tcggccttgg tgcttacata gtgccggtac tcataatcat
    13561 actggggctg gctttggtat atagaaagaa tgaaatccgc attaatatta agtttttttc
    13621 tttctgtata tttttgctgt ccacacttat agtgcttttt ttgtcaaact atacagagat
    13681 tgaaaatgcg gatgccaaat ggctcgatgc tgtcagatac tatttttccc tgggcattct
    13741 caatgcaggg ggaggagcag tgggagcctt gttcggctat gcctttgtaa ggctttttgg
    13801 aatgataggc accatagcga tagtttcgtc actctacata atatcaatac tgatgttcac
    13861 cgaggtatca tccatgcgaa tccttcagta tataaaacaa tccgcatcgg atatctattc
    13921 acgtttaagg aacaaaaaca aagaagccgc agtaacaaag gaattgcaga gaaaaatacc
    13981 cgtaaagcat cacaacgata ttgaatcatc agaagctgac gatgaagaaa gaataaagaa
    14041 catcgataaa aaaataaaga ttcttgactt tacaaaacgt ttcgataagg atttcaaggg
    14101 cacggcggag aatgaagccg atgacaaagc caggaacaag gataaaacaa gcagggaaaa
    14161 cattccaagc atagtacctg tcagcaacat acattacgtg aattacaaac ttcccggcac
    14221 cggcctcctt gcattgccgg cggagaataa gtcaggcaac gggaagaaag aggtaatgaa
    14281 caatgtaaag atacttgagg atactctgaa aaacttcggt gtcagtgcca gcattgcaca
    14341 ggtttctgtc ggcccgacga ttacaagata tgaactgcag ttaagcccgg gggtaaaggt
    14401 aagcaagata ttgagccttg cggatgatat atctctcagc cttgcatccc ccgatataag
    14461 aatagaagcg cccatacccg gaaaagcagc cattggaata gaagtaccca acaaggacat
    14521 aaccagcgtg cttttgaggg aagtgctgga atcggaagag ttcagaaagc ataaatcaaa
    14581 aatatccttt gcattgggaa aagatgtagc aggggcaaat attgtggcgg accttgccaa
    14641 aatgcctcat cttctgattg ccggatctac cggttcaggt aaaagtgtgt gtataaacac
    14701 gcttatcgca agcatattgt ataaagcaac tcccgaagaa gtaaaactcc tgcttataga
    14761 ccccaaagtg gttgaactca gcgtatacaa gaacatcccg caccttttgg tgccggtggt
    14821 gacagatcct aaaaaggccg cgggagcatt gaactgggcc gtaacggaaa tgaccgagcg
    14881 ttacaagaaa tttgcggcaa ataacgtaag ggatatatac ggctataaca atctgaacaa
    14941 ggaagaactg gccaaaatgc cgcaaatagt tgtcgttata gatgaactgg ccgatttgat
    15001 gatggttgcg ccaagtgaag tggaagactc tatctgcaga ttggcgcaga tggcccgtgc
    15061 cgccggaata cacctggttg tggctactca aaggccgtct gttgatgtaa ttacggggct
    15121 tatcaaggca aacatcccct caaggatatc ctttgcggta tcatcacaca ttgattcaag
    15181 aactatactt gatatgggcg gtgcagaaaa gcttttggga aaaggcgatg ccctgttcta
    15241 tcctgtcgga gcggcaaaac ccgtcagggt acagtgtgcc tttgtatccg agaaggaaat
    15301 agaaaagctt gtggcctata ttaaggaaca ggttcctgca agctttgaca ataaaataat
    15361 taaagaaatc gagcaaagtg tagaaattga tgaggacgca gaagctgacg agctgttgaa
    15421 tgatgcaata agaattgttg tggaaaacgg gcaggcgtcg atactcatgc ttcagagaag
    15481 gttaagaata ggctacagca gggcggcaag gctgatagac cagatggagg aacgcaggat
    15541 aatcggaggc tatgaaggca gcaagcccag aaaggttctt ttgggcagag aggacttgga
    15601 agattagttt ttggaaggag cgaatttgtt gagtcttaaa gttgcaatca tatcccttgg
    15661 atgctcaaag aaccttattg attcagaggt tatggtcaag agcatcctta ataatgatta
    15721 tgaagttacg gataatgcca atatcgcgga ggtaataatc ataaacacct gcggtttcat
    15781 agaaagcgca aagcaggaat ccatagacac cattcttgaa atgtccgatt acaagaaaaa
    15841 cggcagatgc aaagtgctta ttgcaacagg ctgccttgcg gaacgctata gggatgagct
    15901 tatcaaagag cttcccgagt tggatgcggt aatcggcacc ggagattata aggacgttcc
    15961 tgaagtgata agtaaagcac tgaagggtga gaaggtagtc ctatacgggc atcaggaact
    16021 gattgatata gataagcttc cccggcacat ttcgggcttc ggtgcatcct cgtaccttaa
    16081 gatagccgaa ggctgcgaca acagatgcag ctattgcata atacctgcct tgaggggaag
    16141 gtaccgcagc cgaaaaacgg aagacatact tgaggaagca aaggagctgt caaggaacgg
    16201 aataaaggaa gtaaatgtca tagctcagga tataaccagg tatggacttg atttgtataa
    16261 aagccggcag ttaagcgaat tgttaaaaaa gctggcggaa atcaatggta tcgaatggat
    16321 aaggcttctt tattcatatc ctgatgaatt caacgatgaa ctcatagaag tcattgcaag
    16381 agaagataaa atctgcaaat accttgatat ccctatacag cataccagca acaggatact
    16441 taaaaagatg ggcaggagaa gcagccgaga gcaaatacaa cggctgattg aaaagctcag
    16501 gaaaagaata ccgggcatcg tacttaggac aactcttatc gtgggatttc cgggagagac
    16561 ggaggaagat ttcaaagagc tgtatgactt tgtttcagaa acgaagttca acaggctggg
    16621 agtatttgca tattcaagag aagaagatac cccggctttt aatatgccga accaggttga
    16681 agaagccgta aagcaggaaa ggctcgaaaa aataatgctt cttcaaaagg atatttcgct
    16741 ggaatacaac cggaagcttg tcggtcaaac actgaaggtg cttgtggaag gtgcttccga
    16801 taatgaatac tctgggcgaa gctgcatgga tgcccctgaa atagacggga aggtttattt
    16861 taaatcgaac aaggaaatta tccccgggga tttctgcagc gtaaccataa aaaaggcata
    16921 tgaatatgac ttaatggggg agagaacaga tgaacctggc aaataaaata acaattatca
    16981 gaatacttat gattccggtt tttttactgt ttgctgtcgc agaatttcct tatcatatga
    17041 cagccgccgt tgcggtattt cttgcagcct cgcttaccga caagctggac ggatatattg
    17101 cccgtaaata caatatgatt accgactttg gcaaattcat ggatcctctt gcagacaaac
    17161 tgcttgtcac agccgctttc ttagtactta tacagctcgg caggatagaa gcatggattg
    17221 ttttcataat acttgcaagg gagtttgccg taaccgggct cagaaccctg gcagccgccc
    17281 agaatatcat tattgccgcc agcagctacg ggaagataaa aacagtaact caaataatag
    17341 caatagtaat actgcttatg gataactatc ccttcagcct attaaatctg cccgttgacg
    17401 ccgtaatgac ctatgccgcg cttttaagta cagtgatatc cggaattgat tattttaaga
    17461 agaatatgca ggttataaca agaatgaaat aatgtctctt taggaggttt ttgcatgaaa
    17521 agcgaaatta ttgcagttgg tacggagttg cttttaggga atatcgtcaa caccaatgcg
    17581 cagtacctgt cacagaagct ggcggatctg gggattgacg tatactatca tgtagttgtg
    17641 ggagataatc tgaaaaggct gaccgaaaca atcaaaacaa gccttgaacg atcggatatt
    17701 gttattacaa gcggcggctt ggggccgaca gcagatgaca ttacaaaaga aggggctgca
    17761 caggctatgg gtttgaagct gctcccggat gaagaaagca tagagagaat aaaaaaaatg
    17821 ttcagttcaa ccggaagaat catgacagaa aacaatatca agcagggata tattcctgaa
    17881 ggcgccgttg tacttgaaaa caacaacggc actgctccgg gtgtgctgat agaaaaagaa
    17941 gggaaaatcg taataatgct gccgggcccg ccgaaagaat tatatccgat gtttgagagc
    18001 aaagtgctcc cctatctcaa gtccaaaaca gattcaacaa tcaggtcgaa agtactccgg
    18061 gtgattggag ttggagagtc agccgttgag cacattctga aggatatatt cgacagccag
    18121 gccaatccga caattgctcc ctacgccaag gacggggaag tacatctgcg aattactgca
    18181 aaaacaggta taccggagga agctgacagt cttattgctg agatggaaca aaaagtaaga
    18241 gcaatacttg gtgacaatat atacggctgc aatgaagaaa cactggaaga ggcggtattg
    18301 aagctgcttc agaaaaaaaa cctgactatt tcactggcgg aatcatgcac cggagggctt
    18361 gtcgcaagcc gcctgacaga tattccggga gcctctgcaa gtttaatctc cggagtcatc
    18421 agttacagta atgaatccaa aataaacatg ctaaaggtta aggaagaaac cataagaaaa
    18481 tacggggctg tgagccctca gacggcggaa gaaatggctg tcggcgcaaa aaggctcagc
    18541 aatacggata tcgggctttc cataaccggt atagcagggc ctgacggagg aagcgtcgaa
    18601 aagccggtag ggctttgtta tataggtata gcaataggca aatccgtaaa tgtgcaaaaa
    18661 atcatgctga ccggaaacag gaacagaata agatgggggt caagcagcag agcccttgac
    18721 tttctcagaa gagaactttt atcaggataa aaacagtaaa attatgtaat tttcttgact
    18781 aacaattagc aatataatat aatagaaaca taagaacatt tgttcgattt gcctcaaggg
    18841 cataagtgat gaaaggacgg tgcaggaatt aatgtgctca gcaatgaacg ataaaaagaa
    18901 agcaatagaa ctggctctca gccaggttga aaagcaattc ggaaaaggct ccattatgaa
    18961 gcttggcgat gccacaaaaa tgaatgttga agctgtttcc acaggctcta tagaacttga
    19021 tatagcattg ggaataggcg gagttcccag aggaagaatt gtcgagatat tcgggcctga
    19081 atcctcggga aagactaccg tagcgctgca tgttattgca gaagcacaga agaacggcgg
    19141 tgaagcagcc tttatagatg cggaacacgc attggatccc ctatatgcca agaatcttgg
    19201 agtggatatc gagaacctga tagtatccca gccggataca ggggagcaag ggcttgaaat
    19261 agcggaaaca cttgtccgga gcggagcaat tgacgtagtt gttgttgact ctgttgcggc
    19321 actggttccc aaagcggaaa tagacggtga aatgggagac gcacatgtgg gactgcaggc
    19381 aagacttatg tcacaggcac tcagaaagct tgccggcgct atcagcaaat ccaggactac
    19441 agttatattc atcaaccagc ttagagaaaa ggttggtgtt atgttcggca atcccgaaac
    19501 aactcccggc ggaagggcgc ttaaatttta tgcttcggtc aggcttgaag tcaggaagat
    19561 agaaaacata aagctcggaa atgatttaac cggaagcagg acaagggtaa aggttgtcaa
    19621 gaataaagtg gcgcctccct tcaagcaggc tgagttcgat atcatgtacg gccagggcat
    19681 ttccaaagag ggcagtattc ttgatgcggc ggtcagcaac gatcttatat tgaaaagcgg
    19741 cgcatggttt tcctatggag atataagaat aggacagggc agggaaaatg caaagcagta
    19801 tctcaaggat aaccccgata tcacaaagga actggataag aagctcaggg aaatgtttaa
    19861 cctgggaata tccaaagcaa aagccgcaga cttcgattcc gaagaaaacg atgaattcta
    19921 atatgagggt gtttccatga gaatcactgg gattgaacaa caaaaaaaga atacgaagag
    19981 atacaatata tatattgacg gagaatacaa atgcaccatt gaagataaca tcctgcagga
    20041 actgaacttg tcaaagggta tggaactgaa tgaaatagag tttaataata ccctggaaat
    20101 aatccaatat aagggcgctc ttagagctgc cctttttatg cttgcccgcg cgccgaaaac
    20161 agaaatggaa attgaaaagg gactcagcga caagcagcat gccccaaagg ccataaaaaa
    20221 agttttggaa tatttgaagg aaataggcta tataaatgat gaaagctacg ccgaaagcta
    20281 tataagaagc ataaaggcta caacaggcac aagccgccga agcatttacc aaaaactcgc
    20341 agctaaagga atagacaagg aaatcattca gcaaatgctt aataaggctg agatagatga
    20401 ttatgcatcc gcaataagtg ccgcaaagaa aaaggcagca agcctgaagg gaaacagaag
    20461 ggaaaagaaa ttaaagcttc ttaactatct ttacagaaaa ggctttggaa ttgatgactg
    20521 caaaagggcg gcagaggata tcttacagga agaagactga aaggcctcca gcaaaattca
    20581 agcaattctg cataacatcc ttaaattgtt tgaattgtca caataacttg acaaaatttt
    20641 gtataagtaa tagaattatt ctgatatggt gtatcaattg agagtgaagc gtagttgcca
    20701 ttatttgtta atgtgaaaat gatgattatg aaaaaggatt aatggaaacc gttaccgata
    20761 atcgataaaa taaaataatg aggaggtgaa aatattatcg aatatgtaat atatatagca
    20821 attgcactaa ttaccttcat ttttgggtat ttagttagaa gatatattgc tgaatcgaaa
    20881 ataaaaaatg ccgaagagca ggcattaaaa atagtcagtg aagctgaaaa gcaatcggaa
    20941 acaaaaaaga aagaagttgt tcttgaggct aaagaagaga ttcacagact tagggcagaa
    21001 ctggacagag agattcgtga cagaagaaac gaaatccaga gagcagaaag aagattgctc
    21061 caaagggaag aaagcctgga caaaaaggtt gagaacctcg agcaaagaga tgaggcacta
    21121 gccaaaaagc aaaaggagat tcagaaatcc ttagaggaag tacaacagct ttataagaaa
    21181 caagtggaag aactggaaag attatcgggg ttatcatctg aagatgcaaa acaactgctt
    21241 ttaaacaata ttgaaaaaca aataaggcat gaagctgcta tgatgatcaa ggatattgag
    21301 accaaggcta aagaagaagc agaaagaaag gccaaggaag tcattgcaag cgcaattcag
    21361 aaatgttcag cggaccatgt ggctgaaact acagtatccg ttgtaccgct gccaaatgat
    21421 gaaatgaagg gcagaataat aggacgagaa ggcagaaata taagaacatt ggaaacactt
    21481 acaggcatcg acttgataat cgatgataca ccagaagccg ttatattatc aggttttgat
    21541 ccaattagaa gggaagttgc aagggttgcc cttgaaaagc tcatagtgga tgggcgaata
    21601 catcctgcca gaatagaaga aatggtggat aaagccaaga aggaagtaga caactatatt
    21661 aaggaacaag gagagcaagc tacctttgat acaggagtcc acggattgca tcctgaaatc
    21721 attaaacttt tgggaagatt gagattcaga accagctacg gacaaaatgt acttaagcat
    21781 tcaatagaag tagcacacct tgcggcatcg atggctgcag agcttggtgc cgatgtaaaa
    21841 acggcaaaaa gagcgggtct gctgcatgac ataggaaaag ccgtagacca tgaagtcgaa
    21901 ggccctcacg ttcagattgg tgccgacctt gctaaaaaat ataaggaatc cgcagatata
    21961 gtacacgcaa tttccgcaca tcacggagat attgaagcta caaccgttga agcagtcctt
    22021 gttcaggcag ctgatgccat atctgctgca agacccggag caagaagaga gaccttggag
    22081 tcatacatta aacgcttgga aaaaatcgaa gaaatagcta attcatttga tggagttgaa
    22141 aaatcttttg ctattcaagc cggcagagaa gtcagaatca tggttaagcc ggaggatgta
    22201 agcgacagtg aaatagtata tatcgccagg gatatcgtca agaaaattga agatgagctg
    22261 gaataccctg gtcagatcaa ggtaaatgtt atcagggaaa ccagggcaat tgaatacgca
    22321 aaatagaaaa tgtgcggcga tacatgcaaa tgcatgaagc cgccattttt aataataata
    22381 attaaaaacg ggtaacacta aacacacggc tgtttcacgg attcctgcgc aataacacag
    22441 ctgattcaga caatggaggt atgcagatga acatattatt tattggtgat gtagtaggca
    22501 ggcccggaag aaattatctg agtacaaatc ttagcaggct tgttgaagaa aataatactg
    22561 attttgtcat aataaacgga gaaaattccg caggaggagt aggcataacc aggagtacat
    22621 atgacgagct tcttagtatg ggagcggata ttataaccct tggcaatcat acctgggcga
    22681 aaaaagaagt gttggaattc atcgaagacg ccgaaaggct tatcaggcct gcaaactatc
    22741 ctgaagacat ccccggaaaa ggatacagaa tactggaaaa ggcagggaaa aggattgctg
    22801 tgataaatct ttgcggcaga gtatatatgg attgtattga ctgccccttt aaggccattg
    22861 ataaaatatt gaatgaactg aagaataaag ctgatatcat aatcgtagac ttccatggag
    22921 aggctacttc tgaaaagctt gcaatgggct ggtatcttga tggcagggtt caagccgtgc
    22981 tgggtacgca cactcacgtc cagacttccg atgaaagaat attgcccggc ggaactgcct
    23041 atattactga tgtcggaatg accggcccca tagattccat actgggagtg gataaagaag
    23101 cagttatcag aaaatttatt accggtatgc ctgcaaaatt tgaaattgca gggggtgagg
    23161 ttgctgttgg ggctgtatgc atacagcttg atgataataa taaagttaca tccataaaaa
    23221 gattcacaca aaattctaaa ttgtagaaaa aaagaggatt ttagataaat ttgtagaatt
    23281 atcagttata tttattatat tttgttactt ttcaataaat cagggggggg ctctatatgg
    23341 atgtattaaa ggtttcagca aaatcgaacc caaattcagt tgcaggtgct ttatcgggtg
    23401 tgttgagaga aaaaggttct gcggagattc aaacaatagg agcgggcgct ttgaaccaag
    23461 caattaaagc aattgccatt gccagaggat ttgtagcacc cggaggaatt gacctgatat
    23521 gcattccggc tttcaccgat atcgaaatag atggtgaaga aagaacagca ttaaaactaa
    23581 ttatagaacc cagataaaaa caatacctgc ttttaacaag caggtattgt ttttttttgt
    23641 ataaaagtgt atactttcct gtataataat tatggaacat ttttggaaaa tcctttgtgt
    23701 attgcataaa ttctaaagaa tttatgatat aataattgca gttcctgcaa ttattatatc
    23761 ttttttagga ggcgcagtat gcaaaaggca gatctgcata ttcatacaac agcatccgac
    23821 gggctcatga gtccggaaaa agtagtacgc tgggcaagct ccaaaagatt gactgccata
    23881 ggaataacag accatgacac tgtcaaggga atcggccctg cagtgaaggc tgcctccggg
    23941 tacggggtgg aaatcgtgcc cggaatagag cttagcactc tatttgaaga agaagagata
    24001 catgttctgg gttattttat tgattacaaa tcccaatggc ttgccgacac tctcaaaaaa
    24061 atacagaatt ccaggtatga gagggccggg aaaatagtag gcaagcttaa cgatatggga
    24121 attgatatta ctcttgaaga agtaaaaggt atagctgatg atggtgcaat gggcaggcct
    24181 catattgcaa gggcaatgat tgataaaggc tatatcagca atatcagaga agcctttgcc
    24241 gaatatatcg gcaaggatcg cccggcttat gtggaaagat acaaactaac ctgcaaagaa
    24301 gcaatagata tgatagaaaa actgggagga atatctgttc ttgctcaccc cgggctgata
    24361 aaaaataagg ataacatcgg aaaaattatt gaccttggaa ttgacggcat tgaagtatac
    24421 cattccaaac acgatgatga gacagtaaaa aatatcctgg ctgttgcaca ttcgaggaat
    24481 ttattgatta cgggaggctc tgactgccac ggaatgaaat ttaataatga accgatattg
    24541 ggaaactatt caattgatta taaatatgta caaatgctaa aagaggcatt gaataaaata
    24601 tgaggagggg tcaaaaatgg agataaaatt atccgacaaa gtcttaaata tagctccatc
    24661 catgactttg gcagttaatg caaagcttaa gcaaatggtt gatgagggtg ttgaagtatt
    24721 tggctttggt gtaggtgagc ctgattttga tacgcctgag tacataagaa atgcagctgt
    24781 tgaagcaatg aacaaaggct ttacaagata tactccggca cagggcacaa tcgatctcag
    24841 aaaagcaata tgtgccaagc ttaaaagaga gaacagcctg gattatgagc cgaatcagat
    24901 aatagtatca agcggtgcga agcattcgct ttcaaatgca tttgcggcaa ttttgaatcc
    24961 gggggatgag gttattgtac ctgttccata ttgggtcagc tacactgaga taatcaagct
    25021 gaatgacgga gttccggttc tggtacagac aaaaaaagaa aacaacttca agatgtccgg
    25081 ggaagagctg aagaatgcaa taactgcaaa gacaaaagca atattgataa attcacccag
    25141 taatcctacg ggtgcagtgt acaccgaagt ggaattaagg gaaattgccg aaattgcggt
    25201 acaaaacaat ctgtttgttg tatcggatga gatatatgaa agaatcatat atgatgataa
    25261 caagcatatc agcattgctt ccttcaacag tgaaatcaaa gatcttacaa tactgataaa
    25321 tggaatgtca aagtcatatg ccatgacggg ctggaggctc ggttatgctg ccggaaatga
    25381 aaaattgatt aaagctatgt ctagtgttca gggtcatgca gtatcccacc ccagttccat
    25441 aacccaatac gctgctgcag cagccttaaa cgggccccag gatgatttga aaaacatggt
    25501 tcaggaattt aataaaagaa gaaactatat gtatggcagg ataaatgcaa tgaaaggcct
    25561 tagatgcata aagcctgaag gagccttcta tgtatatgtt gatataagct cttatctggg
    25621 aagaaatcta tgcggaatca agctggattc ttgtttggat tttgctcagg ttatgcttga
    25681 aaaggggcat gtggcggtgg taccgggggc tgcattcgga actgaaggct ttgtaaggat
    25741 ttcctatgcg acctcaatgg aaattattaa aaacggcctt gacgcctttg aaagctttat
    25801 ctataaaaac attgaatagg gtggtatcat acagtggaca ggaaagaata tcaaaaccct
    25861 ttgataagca gatatgcaag caaggaaatg tcctttatct tttccgagga ctataagttt
    25921 tccacctgga gaaagctgtg ggttgccctt gccgaaagcg agatggagct ggggctgaat
    25981 atttctccgg aacaggtgga ggaattaaga gagcatattt atgatatcaa ttacgaggat
    26041 tcagagaaaa gagaaaagga gacgcggcat gatgtaatgt cccatgttta tgcttacggc
    26101 ttacagtgcc ccaaggcaaa acccataata cacctgggag ccaccagcgc cttcgtaggc
    26161 gacaataccg atgtcattca gatgtcggaa ggcctcaggc tcataagaac caagcttctg
    26221 aatgttatta aaaacctcgc aggctttgca gaaaaatata aaaaccatcc cactttgggg
    26281 tttacccatt ttcagcctgc acaacttacc actgtcggaa aaagaagctg cttgtggatg
    26341 ctcgattttc tgatggatat ggaggagctt gattttcagc tgggcaagct caagctgagg
    26401 ggagtcaaag gtactaccgg tacacaggca agctttatgg cgctttttaa cggtgaccat
    26461 gaaaaaataa aggaattgga cgcaagagtc acaaagaaaa tgggctttga gaaaagcttt
    26521 gaagttacgg gccagacata cccgaggaag ctggacagcc aggttttgaa cgtgctttca
    26581 caaatagccg aatcggcgca taaattcagc aacgatatac ggctcctcca gagcctcaag
    26641 gaaatcgaag agccctttga aaaaggacag ataggctcgt cggcaatggc ttataaacga
    26701 aatcccatga gatgcgagag gatcgcagcc ctttcaagat tcgtgataac aaattcgttg
    26761 aatcctgcaa taactgcggc tgctcaatgg tttgaaagaa cccttgatga ttccgccaat
    26821 aaaaggcttg caattccgca gggttttctg gcggttgaca gcatactgaa cattatgata
    26881 aatgttacgg ccggccttgt agttaatgaa aagatgataa aaaggcacat cgacgaggaa
    26941 cttcctttca tggctaccga gaacatactt atggaagcag tgaaagcagg aggggacagg
    27001 caggaactcc atgaaaaaat aaggcagttc tctatggaag caggaaaaag agtgaaggaa
    27061 gagggcctgg acaacaatct gataagcctt atagaagggg atgaaagctt caggttgggc
    27121 aagaataaga tacaggagct tttggatgcc ggaaacttta tcggaagagc cccggaacag
    27181 gttgaagagt ttcttgcgga acatgtttat ccgcttcttg aaaaaaacaa ggataaatta
    27241 aatattacag cagacttaaa agtataaata taatcacaca ttaggaggag agcatatgag
    27301 cagcataccc ataaaacaac tgtacaggag cacagaagat tacatcagca agaatatcaa
    27361 ggtatccggg tgggtaaaga ctgaaagaac ctcaaaaaat tttggtttta ttgaattgaa
    27421 tgacggaagc ttttttaaga accttcagat tgtgtttgaa gaagaccttg aaaacttcaa
    27481 tgacatagcc aaactttcgg taggttccgc cataatcgca gaaggattgc tgacggagtc
    27541 tttaggcgca aagcagccct ttgagctgaa agccgttaga attacaatag aaggcaaatc
    27601 tacacccgat tatccgcttc agaagaagaa tcattccttt gaattcttaa gaacgatagc
    27661 tcacctgagg ccaaggacaa atacattttc cgcggtattc agagtgagat ccctggtagc
    27721 atatgcaata cacaggtttt ttcaggagca gggttttgta tatgttcatg cccctatcat
    27781 tacaggaagc gatgccgaag gggcaggaga aatgttcagg gttaccaccc tggatataga
    27841 taatcccccc aggacagaaa aaggagaagt tgacttctcc agggatttct ttggaaaatc
    27901 cgcgaacctt accgtaagcg gccagcttaa tgcagaaacc ttttgcatgg ctttcaggaa
    27961 cgtatatacc ttcggcccca ccttcagggc agaaaactca aataccgcaa ggcatgccgc
    28021 agaattttgg atgatagaac cggaaatagc ctttgcggag ctttcggaca atatggaact
    28081 ggcggaaaat atggtaaagt acataatcaa ttatgttctt gagaatgcgc cggaagagat
    28141 ggaatttttc aacaggttca ttgacaatac tctgctcgat aggctgaaga atattgtaaa
    28201 ctccgatttc ggtattgtta cttatacgga agccatagac atactgaaaa aatccggtgt
    28261 agattttgaa taccccgtcg agtggggaat cgacctgcag acggagcatg aaaggtatat
    28321 caccgagcag gtctttaaaa aaccggtgtt cgtaatcaac tatccgaagg aaattaaagc
    28381 tttttatatg agaatgaacg atgacggaaa aacagtagct gcaatggacc ttctggttcc
    28441 cggagtggga gaaatcatcg gaggcagcca gagagaggaa aggcttgagg tacttgaaaa
    28501 gaaaatggat gaaatgggac tgaataaaga ggattactgg tggtatcttg atataagaaa
    28561 atacggcggt gtcaaacatg ccggctatgg gctgggtttt gagagaatta taatgtacat
    28621 aaccggaatg ggaaatataa gggatgtagt gcctttcccg agaacggtta aatcagctga
    28681 attttaatgg gacgcgggga cgcggggacg tttctcccgg ggacatgggg acgtttctcc
    28741 tgtccccgtg tcctcgtgtc ctattgtaac taaataagac aacgcctcat attatggaat
    28801 aagggttcat ttcattccat atacctttga agggaggctt aataatgggt cgtaggcact
    28861 cgtcggcata taatgcatta cctccggctg catgaccgcc gtcatcatat ccggcggctt
    28921 cgccggcacg aagcctgttt cccctgcctt tttccctgtc tgcgcttccg acaggggaat
    28981 tgttaggagg aatcagcaac accgtaagag ggtttgtttt aaatgacaac tggtgggtta
    29041 tactgcttat aattttttta tttttcccca aggtcagcgg caggatgttt ggagggcttc
    29101 ttggaaaata gactcctgag aacctgaagt tattcggttt tttggatata aacagctatt
    29161 gactaagcat gttttttttt atataatgaa taagtgtcac aggggtatag ctcaaccggt
    29221 agagtagcgg tctccaaaac cgttggttgt gggttcaagt cctactgccc ctgccattat
    29281 atgattctta cagccgcaat tgtttgcggc tttttttatt tttgatttat ttccgtttta
    29341 ttattccact ccgattgcaa ttgtataaca tgaaagctca attgtatttt tatacaaaaa
    29401 tgagcttttt tttgaacctt ttgaactcag cgtatcggct atgacatatc aaaaatgccc
    29461 ggagcttata agctgtatct tgcagtggct ctctacaggc ttctgaatta atctcctttt
    29521 atatcaataa aaactttttt gtgttactcc ccattttctc aaggtttcat cattgcctat
    29581 ccatacaggt ataccaaccg gtataatatt ataaagactc aggacatctg aatttatcat
    29641 gcggatgcag ccttttgaca cctggcgccc aatggaatag gggctgttat ttccatgaat
    29701 accgtacaaa ttgccttttc cgacagaaag gcctatccat cttgttccaa gagggttctt
    29761 cggggaaccg ccggctacgg gttttgaacc gtcgcccgga gaccatatcg ggttctttct
    29821 tttcaggcat acatggaatt tcccctgggg ggttttgttt gtagcttttc ctattgctat
    29881 cggatacttt ccaatgactg tttttccgct gtacaaagta agaattcttt ttgtacggtt
    29941 aataactatc caataaccgc gggaggcagg ccttgcgata atatccggag gcctgtaatt
    30001 accgtttaat gaaagcctct ttatcatggc agccttgaat tggggccctt gtatgccgtc
    30061 ggcggcaaga ttgcagtcgc tttgaaacct gagagttgca tttctcaaat taattaattc
    30121 atctttataa tcatacttat aatagcccat tgattttaat gcttctatat aattatcact
    30181 gttcaacccg gaaaatgttt cttcctcggg acaggccatg agttctttga taagctcagt
    30241 atcttccgca taaatatcaa tagaaaatgc cggaaataac agcaataccg cagcgattaa
    30301 acctttgcgc ataaaatacc tccttgaaag catcttaaca ttattattat tctgaaaaaa
    30361 atgctttctt atgtccgtgt ttgaatattt acatatactt catattcata gaataatgcg
    30421 acggggacac ggggacgttt cccctgtccc acttccagtg ggacacgcga aacgtccccg
    30481 tgtccccgcg tccccatgtc ctcgcgtccc catgtcctcg cgtccccatg tcctcatata
    30541 ataaggagag ctaattgctc tccttgtttt aagtattaga accttgttcc ggttgttcct
    30601 gccatattcc tctcagctgt ctcaattagt cttctaacca tctgaccgcc aattttccct
    30661 gcatcttttg ctgcaatatc tccgttgtaa ccttccttta aagtaacgcc aacctctgtt
    30721 gctatttcat atttaaatct gtcaagagct gctgctgcat gaggtaccac cttctgatta
    30781 tttcttgcca tccgtattca cctccttgag caatatgtat aatgagttaa taatgattta
    30841 cgcattatta ttttggttaa gcttcaatat tttaatccaa gtaaattttg cctgatttgg
    30901 ctcattaagt gattcattaa tctacctgaa aatccatcac aaaataatca ttaataatgt
    30961 aaataataat aatgtcatta ttaatgacaa tacaaaacga agggagatca ggaaatgaaa
    31021 ggcgtaaaac cttcagtaaa agcactttgc agtgccttgg cagtctgtat tatcttttct
    31081 gcaggcattg cagcctttgc cgacaacaat gctccatcag cagtcaaaag caaacactac
    31141 ctggaagagg ctgttgaaaa aatggtgcag gaaggaagct taaccaggga aaaggcggat
    31201 aaaatacttg aatacaaaca aaaaagaaat gagaaactaa agaagctgcc tatagaacaa
    31261 aaaacccaaa tgaaaaaaca aggcggaaga gggagtcttc tgaaggcact ggttgaggaa
    31321 ggcataataa cggaagagga agcaaaatcc atcagggaaa agctgcggga aatgaaggaa
    31381 gcaagagttg ctgacggatt gcagggactt atagataaag gagtgcttac gaaagatgac
    31441 atcaataaca tcagaagcta tttgctgaaa gtcagggaag agcgcaagtc atccgtcgaa
    31501 aagctgaaat ccatgactcc cgaagaaaga aaagcatact ttaaagagca taaaaatgaa
    31561 cgaaaagaca tcattaccaa gatggtggaa gacaaggtaa taacaaaaga acaggcggaa
    31621 gaaatcagaa aggcaatacc ggaattaagc aagttcagat actaggccat gacacagttt
    31681 aggagattca gggcagggat tttaagttag gataatgtca aataccgacg tgggggaaga
    31741 tatcttcaag gaaaccttct tcccctacag tcggagtact ttacaaaccc tctctatgcc
    31801 attggtaaat caatgcggaa tacggctccc gcctctgaat tctccgcagt tatttttcct
    31861 ttatgttttt caattataca cttgcttatt gcaagaccta ggccaaaatt acccttccgg
    31921 cctttaaaga acctgtcaaa aatattggga agctcattgg attcaaagcc cggcccgtca
    31981 tcggatatcg tgactcttac tgttttttca tcaataatct ctgactttat ttcaattgag
    32041 ctttttgcat agcgtatgca attgctgagt atattgttga ttgctcttga aagcttttcc
    32101 tcgtctgcaa ttatattaat attttcttca ggcacagata tgtcaatttt gatattgtta
    32161 ttgcaggcaa tcatattcat acgctctgcg cagctgcgaa gcagttcgtt atagtccagc
    32221 gtcgtgagac aatagttttc ctccattgca tccagccgtg acaggtagag caggttttcc
    32281 acaagctgag taaggcgttt ggattcttca attatgatgc cggcagcttc attgctgtcc
    32341 gcaacattgt attttatgcc ctccgcatag ctctggatgg acataagcgg agtcctgaac
    32401 tcatgagaaa cattctgcaa aaaggtcttc tgtgccttgt cataagtctc cagcctttcg
    32461 gacataatat ttatgttagt cacaaactcg ctcagctcat cgtacacggg agtgctgatc
    32521 ttcgctccga aattcctttc agcaattgat cgtatatgct gattcaagga actgaacggc
    32581 tcagagattt ttttgactat gacggaggag aacagtacaa taataaatgc cgacaacaga
    32641 agtattgcga agagaataat atttatagcc atctgaagct gatttacctt ctgcaggctt
    32701 gagtatatta ttatccaccc caatccgaaa tcattcttct gcgctaccgg ttttactact
    32761 gctatatatt cggtgccgga aagatggaag ttcaagtagc tttccttttc cagatccttt
    32821 aatttgctta tctctgagga aattttacct acgaggccct catcgggatc cgcatacaat
    32881 tcaacaggtg aattaataat gtttttatta ttatccatta aaatataatc ggcattcaat
    32941 accgaaagcg gttctctcaa ggatctgtca agcatgaagt aaaacctgaa cacgtcattg
    33001 gttcttattc ttatccttgg aagctcagga ggtatttcct tgctgaatat gaatatttca
    33061 gggccccttt tcaaagccgt ttcttcggca tgggatgcta ttttattcaa ttgccctatt
    33121 atatcctttt caatatagat ccttatggca gcattaaata tgattgcaac cagcacaatc
    33181 aaagtgatta taacggtaag attgtatttg aatattcgcc attttatggt attgcttttc
    33241 ataatatcat tccttgcctg ttaatcggaa gccaaagccc catactgtat ctattttaag
    33301 ccttgagcct gcatctgcaa gctttttccg tattctctta accatgtcat ctgtagccct
    33361 ggtttctact tcgctttcaa agccccagac cttgtcaaga agctcactgc ggcttacagc
    33421 cctgtttctg ttagctgcaa ggtaataaag cagtgagaat tccattcccg taaacccgat
    33481 atccttatca tggcattttg ccttcttaat attatgatca ataacaatat cggagacttc
    33541 tataacttct ccgattcctg tctgagcttt atccagttca attcttctga aaatgcttct
    33601 gattcttgca ataagttcca tagggctgaa gggttttgtc aaataatcgt cacatccaag
    33661 ggtcaatccg gtaatcttat ccggctcagt atccttggct gatactataa taatcggaac
    33721 actgcttatt tgccgtacct gacggcatag agagtacccg tctattcccg gcatcattat
    33781 atcaattaca agcatgtccg cgggtttgac attaaaagcg tcaagtatgg attgcccgtc
    33841 gttaaatgtt tcggcctcaa aaccttcctt aataagaaag gattttatta tattgcatat
    33901 attaacctca tcatcagcta tgtataccag ctttttatcc ataggctcat cacctcatga
    33961 ttaattttat catgaaaacc tgtaaaattg catttgaaaa ttccttctgc tttaaatttg
    34021 ccgcagtttt gcggagattt ttaaatagga aaagcgatag aattatacat gtaacaagtc
    34081 aaaagctaag aaacaggggg tagcatcaat gaattcgaac acagttccag tcagaaagca
    34141 tttaataaaa ttatcgagga aaaaggtact gattgctgct gcaatactgc ttgtggctgc
    34201 atcagtgata ggattcaact attataaatc aaaaaactct aagaatccgg ccgtacagca
    34261 ggcaacagcc atagcaagaa tgggggacct tagtgtcgtt atcacaggat cagggccaat
    34321 cagttcctct gaaaagttta cattgacatc caatgtaaac gggactctca caaaaatata
    34381 tttccaggat ggagataaag taaatgcagg agatttgata tttgaaatag atgacaagga
    34441 tacccaactg cagataaaac agataaaaaa cagcattgca caggcagagc ttacacggag
    34501 cagcaatata tcggaccttg aagccggaac ggttacagct cctattgacg gggagataca
    34561 tgacatgcag gttaaagaag gagataacat atcaaataac ggtactttat ttgtcattac
    34621 ggacaaatcg gaattaaaat tgttggttcc gtttatcaat acatacagaa acaggctttc
    34681 catagggcag aaagttgcaa taaatgcttt tgataccgaa agggacgaac tgcacaggat
    34741 ggagggaacc atatccggca tcagtactcc cggttacaca acaagcagcg gtgctgaatc
    34801 atataatgtg agcatagtaa ttgaaaataa cggctccttg aaagaaggaa tgattgcaaa
    34861 tgcttccgta gacatagacg gaaccgaggt aaaaagcagc ggaagcaata cgctgagtta
    34921 cttgaaaaac atgaccgtaa gggccccttc gggaggaact gtaagcaagc tatatgcaga
    34981 aaacggtcag aacgttaaga agggtgatat cctggcagag cttgaaaata aagacctgga
    35041 gcttgctatc aaaacaaatg acctgaaatt ggaggatttg aatatacagc tgcaaacagc
    35101 ggaagagaag cttttggatc ataagatata cgcgcccttt agcggaacct taacactgaa
    35161 tgatattgag cagggcaaca gcataaagca gggggatata ctgggaagta ttgcaaacta
    35221 tgacattatg gagttcagca taaacgtgga tgaactggat attgccaaaa taaaggaagg
    35281 acagaatgta agagttacca tagatgctct cccggataca actgacaaac cactcaaggg
    35341 aattgtatca aaaattgcag tagagggtac atcgtcaaac ggagtatcaa tctatccggt
    35401 aacaattaaa attgagaaaa atgaagcact gaagggcagt atgagtgcaa acggggaaat
    35461 aatcgtgaat gagaaaaaag atgtacttta tgtaccgatt gatgcagtgg ttaaaatgaa
    35521 cggtaaagac tatgtaaggg ttgtacccgg ggcaggcggg gcaggtaaag ctgccagaaa
    35581 ccggaattca gacgcaaacg gcacagaagt taaagaagac acaaagcaga atactgaact
    35641 gcaagttgaa ctgagagagg tcaaaacagg tataagcaat gccgaataca tagaaatagt
    35701 cagcgggctg aaagagagag aagcagtaat cgtaacttca agaagctccg gcagcaataa
    35761 tcagagaggg ccgaatatga tgattatggg tactcctcct gcaggaggcg gcaatgtcag
    35821 aatcagaaac aattaggagg agtgcggtca tgatagaaat atgcaatatg agtaaaattt
    35881 atcggatcgg aaatgtcgcg gtaaaggcat tggataatat aagcctgagc attaagcagc
    35941 atgaatttgt ttcgatagta gggccttccg ggtcaggaaa atccactctg atgaatatga
    36001 tcggctgcct tgatgtacct acctccggag aatactgcct ggacggcatg gaagtaagcg
    36061 gcctgaccga cgaccagctt gcatatatac ggaatcataa aataggcttt gtttttcaag
    36121 gctttaatct gattcaaaaa cttaatgcct tggaaaacgt tgagcttccc ctgatatatc
    36181 aaggcatacc cacaatggaa cggtatgaaa gatcggtgga agcactgaag ttggttggct
    36241 tggaggaccg gatccatcac aggccggcag agctttccgg cgggcagcag cagcgtgttg
    36301 caattgcaag agcactggtt ggcaatcccc agattatctt ggcagacgag ccgacgggaa
    36361 acctggattc aaaatcaggc aaggacataa ttaatttgct taaggagctg catctgaaag
    36421 gaagcactat agtcttgata acccatgaca atgatattgc catgatggct gagagaatag
    36481 tccggattca ggacggtcaa ataaacgatg acaggatggt gagctaggaa tgagttttat
    36541 tcaggcctgc aaaatggctg taaagagcat caaaagcaac aaagttcgct ccttcctcac
    36601 catgctggga gtaataatcg gcgtatcctc tgttattgct gcagtaggct ttgcccaggg
    36661 cagcaccaaa agtataacgg atatgatatc aagtatgggc accaatctga tacaagttaa
    36721 tataatggga agaaacagca acagaaacgt tacatacagc cagataaagg aatttgccga
    36781 agaaaacagc ggggaaatta cggcagtggc accccagtca aacacttcgg ctacagtaaa
    36841 atataaaaca aacagcagga gcaccaacgt tcttggaaca agccccgact accagataat
    36901 aagaagtgtt tatgcgcagc agggccggtt cctgattcag aatgaccttg agaatatgca
    36961 gaaagttgct gtattaggaa ctgcagttgt caatgacatt tttgaaggaa caagccctct
    37021 tggggaaaaa ataaaaataa acgggcagct ctttactgtt gttggcatac tggaagagaa
    37081 ggacggagga caggaccagg gagcagatga cattgtaata attcctgcca ccgttgcgca
    37141 gaggctgttc aggtcaggga taatcaggaa tttttccata caggcaacta gcccggacac
    37201 agtggaaagc gcaatggaaa aacttgagga attccttgca aaaacctata aggattccga
    37261 tgctttcagg gtctttaacc aggcagagat gctggagaac cttgacagca tgaccggaac
    37321 catgacagcg gtactggcgg ggattgcagc tatctcactt attgtcggcg gcataggaat
    37381 aatgaatatc atgcttgttt ctgtcaccga gaggacaaag gagataggca taaggaaggc
    37441 cataggagca agaaagaaaa acatacttgt tcaatttctg attgaagcaa taattcttac
    37501 cggattcggc ggagtcctgg gtgtggctgt cggcctgtcc gtaatacatt ttattatcgg
    37561 aggattcggc cttgtaccgg aagtatattc gataccctgg atattgattt ccttcgggat
    37621 ttccctgggg gtaggcgtta ccttcggatt gtttccggca tataaagcag ccagcctcaa
    37681 tcctatagaa gctttaaggt ctgaataacg ggaggagaaa gatgagaaga acatttttta
    37741 tattatctgc catactttgc gtatcattat tatttaactg cattggtgca ttcgccgatg
    37801 caacggagga cgggctgtta accctggagg aagccaaagc aatggcactc aagaatgacg
    37861 tgcattatca ccatcaggat gaaaatattc aggctgcatt agaggattat gaaaaactaa
    37921 gcgaagatac atcgataaac tccggaagag gaagcaacat tgctcaaaaa gaagcagcac
    37981 gcatttcgaa gaaaatccag cttgaaaatg catactccag cgtccgcaaa gtagttttga
    38041 caaaggcaga tttgaaaaga gcatcatact ccgatacggc aaatgcattc tatgctgtta
    38101 tagaagcgca gaattccctt gcaagcgcat tgacagattt agaattgaag agaaaggagc
    38161 tggaaaaggc tgaaatcaaa tatatgctga atattgtccc taagaaaacc cttacgcaag
    38221 cagaggaggc atgcaaaaac tcagaagctg cgtataacaa ggcagccctt gagcttgaaa
    38281 actgtatatc aaagctttgc aaaatcatag gccggaaatt ggatatgtcc aaggtcaagc
    38341 tggataccac acttgatatc cccgatataa aaagcattga tcttgataaa attaaggaag
    38401 acaatctgaa aaacaatctt agctattttt ctgcccggga acagtataag ctggctgagt
    38461 accaactggc actgactgaa gaaaaatatg aagattacta cgatgacctg aaaaagggtt
    38521 cctcaaatgt cagggaagag tttgacaaca tgctctatga tgcgcaaaaa aagttcgaag
    38581 atgcggagta cagctggaat gagaagctgg aggacctgga ggagtccctc agggaacagt
    38641 atgagagcct tgaggaccta tatgaaaact atttggagca aaaggaagac attgatgata
    38701 tgaagcttac cgtaaaggaa aacaaaacaa agcatgctat gagcattatt accaaagctg
    38761 ctctggacag cagcattgcc agccttgaaa ggctggaaag gcaattggtt tcaacaatta
    38821 taaagctaaa caagcagtat attagtttag tacagtgtag tatcgaggaa taagctgaat
    38881 ttacagccct gacggttcat caaatgtccc gtcattaagt actatcattc tcatttgata
    38941 aagcttatca ccattaaata attgaaaata ctatatactt tattttcatt tgcaattgac
    39001 aaaagttaat atatgtaata ttattataga agaaaagtat ataccggaat agaacaggcg
    39061 gattgaatac aatttagccg ggctaaaatt tagcagcagg ttaatatact tgtgggacta
    39121 taaaacagtc ttgcaagtat tttttttata ggactgctgg gtattgattc atttaagatg
    39181 tattggggga cgaaattatt gaataatctg gtaagcaaaa tcaccgatct gtttattata
    39241 aaatttatta aaaacaccaa gccggaaaaa aaaatatcag accctgcaac taggcgcaaa
    39301 tatgcttatt tggaggcata tgtaagtatt gtcggcaatc tgctgctgtc cgccgtaaag
    39361 gttgcactgg gattattcct taacagtatt tcccttttgg ccgatgcagc ccatactgcg
    39421 gcagatgtat tgacctcaat agtcgtactt ttcggattca agctgtcaag ctcacctgca
    39481 gatgagaagc atccttttgg gcacggcaga atagaactga tcgcaacctt ggtgatttct
    39541 gcaatgcttt ttttagtagg ctttgaattt gcgaaatctt cttatgaacg ccttttatca
    39601 aacactccgg tcaaaggcag ttatattata gcattaataa tgatatttgg agccatattc
    39661 aaagaatgga tggccagatt ctccatagac cttggcagca gaacctcctc atcaaccctt
    39721 attgcggatg catggcacca caggaccgac ggaatagcct caatactggt ggctgctgca
    39781 ataatagctt cgaaattcgg ttattataaa gtggatgccg ttttcgggct tatggtatcc
    39841 gcgctgataa tctatacagg gtatagtatt tcaaaagatt ccatttcaca aatcattggg
    39901 gaaacaccgg cggaagagga actcaataat attgaaaata ttgccttgac tgtatcggga
    39961 gttcacagta ttcataaggt gaatgtccac agttatggct gccataagga aatatccctg
    40021 catgtacagg tggataatga tatgtccctg gttatggctc atgatatttc cgagcaggtg
    40081 gaaagaataa tagaagaaaa tatgaactgc aaggcaatcg tccatataga accccttgag
    40141 taggtttatt agcacggagg aggcaatagc ccggaccgga cccttggctg caaatcctta
    40201 tcagaagaat atgtccatat aatatggaaa tattcaagta attttttgag acaatatatc
    40261 gctaatgata atcgttctca tttaaaataa ttgagtctga agcagcagcc ataaaataac
    40321 caaaaaacag acaataatat tttttagtaa tttatgcagg cgcgatacat tttatatttt
    40381 taagaattaa atgcatttag cacatttttc tgagattata atttacttag tttaaatttt
    40441 tcttggataa taattcacag cgattaaaat ttaccgaaat atgatttgtt gaagaagctt
    40501 gacacgtaat tcaatatgtt ttctaatact tttttcttga agcacccagg agtattgata
    40561 ttgctgatgt ttcaaaaaac cctcaaaaaa acgacgcgtt taaatcgctt cagagcgatt
    40621 ttttttgata gggtaatata attagacctc gtgattttta ttagcagaat acgcagccgg
    40681 tatccgggca gcattttgcc tggtccataa gggcaattat aaatttcccg gctaaaggtg
    40741 attttggggg tcaatcaaga aagggaaatt ataagtaatc acgaaatagt aaattaatga
    40801 gcatttagta ttgcagataa tcaaaaactc gtgcagaaaa cgccagttta aacaatttat
    40861 aaaactttat aaataattta tcaataaaag aggattttac ctcttctgtg tcgaatactt
    40921 taattgcgct aaataatcat ttcgcgcaat attgaataaa taatctaaaa aggggatatt
    40981 taatatgact aagcttcctg atattgcagt tgaatttctt gaatacatgg aaaccatccg
    41041 cggtaaatca aaaaatacta tcaccgctta tagttatgac cttgctctct tttttaaata
    41101 tataaaaatg aaacgcggtt atttagtaac cgatatggaa attgatgaaa ttcccatcga
    41161 tgatgtagat atgtatatga tcaacaatat aagccttgct gacttatatt ccttcctctc
    41221 cttcgcggct aaccagagag acaataaaaa ttacgccagg gccagaaagg ttgcatgtat
    41281 aagatccttt ttcaaatacc ttcataaaat gaaaatgatc aaagaaaacc caagcgtaga
    41341 actggaatca ccgaaaataa attccaggca tcctatttac ctcactcttg aggaaagcaa
    41401 gaagcttctt tcgtccatag acggcgagaa caaagaaagg gattacgcta taataactct
    41461 cttcttaaac tgcggactgc gcctttcaga actaataagc atagatataa acaaaatcag
    41521 cggtgacata ctgaccgtaa tcggaaaagg cagcaaagaa cggacggtat atctgaacaa
    41581 ggcatgcata gatgccataa ataactactt aaaggtccgc cctgttgatg atgttaaaga
    41641 taagaatgca ttgttcttaa gtgaaagaaa gcgcagaatc agcaagagaa ccgtacaata
    41701 ccttgtgaaa aaatatatcg gtatttccaa ccttgatatg gaaaaatact cccctcataa
    41761 gttaaggcat actgctgcca ctctaatgta caagtacgga aaagtcgata tcaggtctct
    41821 tcagcagatt cttgggcatg aaaacatttc aacaacacag atttatacgc atttagatga
    41881 tgaaactctc aggaaagcaa taaatgccaa tcccctttcc gaggagaaaa aataaatctt
    41941 cgaaatgatt aacataaata tttttatgtt aatcatttcc ttctttttca ctgtaatcca
    42001 ctatatagtc tctctcatat ttccattttc catgatcttc catatccaac tctgcatctt
    42061 cctcttctat tatctcgtct atctcaatgc tgttgtaatc agcagcctta tctatgcaag
    42121 tgcagcaatt atcgcaaatc ttatttggat caatgtcaca tctgtcgcat tctccgcagt
    42181 cggtacattc tctgtccgtt aaaacacact ctttcttgta ttccatatat cttcctcccc
    42241 aatttatacg tcagctctat tttacaatgt attggcacta aaatgcaatc cggtcattaa
    42301 cttgggaaat tcttctataa tctattcaga gaagaatata ttatgccgat attatattta
    42361 aatgcttgct ttatatttgt ataagaaggc ggtgctaata ttgattaatg cttctgctaa
    42421 tatttttgtt tttacagctt atgtttcctt gctgataatt gagtttgttt ttttgaaaat
    42481 actatatatt gacttcaaca ctccctggct tattaccaca ttaatatatg ttttgttcat
    42541 aatatatcag atactggtca gagttaatga acaagtggaa aaaggcagag taaaggaaat
    42601 tctgtattgt atcattgact ctattaatgg gataccgctg gtcaataagg ataataaaat
    42661 aattatacaa tgggatgtaa tttttaaaaa actgattgat aaaaaagaat actttctata
    42721 tgacaatatt ataaagataa aaacaaaaaa cattgaccga tatgttaacc agtatccctc
    42781 cctcctattt aacagctcca tggatatgct ggaaagaaaa aacaagattg acacaaaatc
    42841 tattattaaa ggaaaagaat ccgccccgaa agagttatcg gaagaactgg atttaaatga
    42901 agagatgaaa aaagtgttgt atgtcaaata tttgagcgct aataaaatga cactggctaa
    42961 tcttgattct tccgtttact ttgatattgt gttaaatgat gaatatgagc cccctgaatt
    43021 tgaaaaattc ggtatcaaac ctgatgacag atatgatgga ttttcattgg ttgcttatca
    43081 taaggataag aagtacattc tggttccaag gataatggta tattgcaaaa gaatcgacaa
    43141 ggaaaagtgg ctgtatcgaa atatagacag aatcataaaa tgtgccgaaa atatagctgc
    43201 aggtatacag gattactcaa taattgtaga tgagctgagt ttaaaagatg taacgaccga
    43261 ttactatgag gaagaaaccg aagtaatacc gcctttctat tttgagcgct tagacaataa
    43321 catcctggag ttttccggaa taggaaaagg cagggaagtt gcaagggctt actgttcatt
    43381 ggatacatct cataatagct ttgaaatcaa tctgcaaaaa tatgagttga ttattgatgg
    43441 tgtcagcgaa gttgtagaag aagaaaaaac aatcgccata ggaaatgcgt atcaacctgt
    43501 tgttgacata atggagaaaa tgccgataat atccaataac attaaactgg ctatattaaa
    43561 gaaagatatc gaagaccttg taattaaata agtattctat ttgaagcctg gaaaatataa
    43621 aagttctccg ggcttaatat ttgcgttctg caggccattg atctccataa cccttgatat
    43681 atatttcctt atatccatat cccccttatg cttttgtgac atgttccaca gtgtatcccc
    43741 cacttccaca tattcaggaa tcatcgtgac atccttattc tccctggcac ttactgcaag
    43801 cgtaaaaagc gaggacattg caaatatcaa tataattcca acagaaacta taaaccttga
    43861 tttgcttctg ataacatacc ttttcatata attacctcca tagcgaacat ttgttctttt
    43921 ctccattata tgcgaacata tgtactgtgt caagaaaaaa attaaaaatt tacgaacgta
    43981 tgtttgaaat ttttaacttg ttatgttata atgatagaag aaattaatgg gaattatata
    44041 ttggagagta atatgaatat tgatgattta agtaaaaagc aatttgaagt cctgaatttc
    44101 ataaaaaaag aaatcaacaa aaaaggatat cccccctccg tcagggaaat atgtgatgct
    44161 gtgaagctta aatccacttc taccgttcac ggccatcttg agagacttga gagaaaggga
    44221 tatatccgaa gagacccgac aaagcctcgt gccattgaag tcctcgacag caacagctct
    44281 attgttacaa aaaaggatat ggtagagctg cctgtagtcg gcaaggttac agcaggcctt
    44341 cccatacttg ccgaagaaaa tatcgaagat acatttccgg tgcctataga ttttgtcgga
    44401 aacagtgaag cttttatgct gattgtaaag ggtgacagca tgataaatgc cggtatattt
    44461 gacggtgatt ttgtaattgt caggaagcag tcagtcgcca ggaacgggga tattgtagta
    44521 gcattgttaa atgatgaagc tacagtcaag accttttaca aagaaaagga ttatttcagg
    44581 ctgcagccgg agaatcccta cctcatgccg attattgcaa aggagctttc gattctggga
    44641 aaagttatag gagttttcag atccataaaa taaaaataat ccgataaccg caatatacat
    44701 tctaataggt tatcggatct ttattttttg tttttatttc ttcggacagg aatccaaact
    44761 tcactgtaag cgtaatcttt tttattttta tccatcttag taaatgaaaa ggacggagta
    44821 ttgattactt cgtaatccga cgaaggtagc cattctgaat atattcttgc cattgtattc
    44881 tgcagcgtgg aaggaaatga tcctttattc ggaaatacgg cccaggtaca ggcttctacg
    44941 tgcacttttt ccaatagatc acttacatta ttttcggtgg ttaatatacc tatcaggtga
    45001 gttaactctc cttcttctct taagaagtca gcatcagcat cataagaagc attgacaatc
    45061 tcataaggct ccatattttg aagagaatgc atttcttttt tttgttcctc tgtaatgctt
    45121 tctgcaagct ttacaatttc tttgttgata ccttcaaatt gcatagggac acgtttactt
    45181 acaccgacta aattaaacgc cggtttgtcc tcaattctaa attccatatt cattcctccc
    45241 ttgaacgatt aatccggtac actccttact ttggtgcctt ctttcagtgt ctcatccggt
    45301 gacagaatta tttcctcttc ttctgtcaag cctcttaaca cctcaacctg ctcgctgctc
    45361 tcgattccct tttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncttctg
    45481 tcaagcctct taacacctca acctgctcgc tgctctcgat tcccttttcg atagccctta
    45541 gttttgctgt tccgccttca ttgacaaata cgtggtcttt gcccttgtag ctgaacacag
    45601 ctttctcatc tataaggaga gtgtcttttt tacttttcac aataattttt acggtcattt
    45661 catatcccgg cctcagccct gctgcttcat tatccaagct tatttccacc ttgactcttt
    45721 tctgttctat accaagatca gacatgatgc tttcagcttt gggatgtatt tcccttacag
    45781 tacctttaat atcttgcata ccaatttctt cgtcatcaat tattactttt gaacccacac
    45841 taactcctgc aatatcctca ataagcacgt tgctttcaat ataatatcct tctgagcctc
    45901 ctatttcaaa tagctttgac cctggctgca caatactgcc ttcttctgct tcggcagtta
    45961 tcaccaagcc ctctataggg gatgtgacaa tcatttcttc ggatttcagc ttaagctgtt
    46021 ctattctggc attaatttcg gaaagctgag cttcatattg tttttttatg ttccccgaga
    46081 tacccttctc agcaagtgcc agattgcttt ttgcagtttc cagccctgct tctgcagctg
    46141 caaagttggt aagagagttc ttataagtgc ccaggcttat tgctccggct tcatataaca
    46201 ctttattgct atccattgtc ctttttgctt cttcatatac agcttctgca gagcgcacct
    46261 gtgcattcag gatacgtatt gtttcatcat ccgccgaggt tttagcttct gcatattttg
    46321 cttctatccc aagcttttgt gcttctaatg cttttatctg cagctttata tcgctgttat
    46381 caattttggc aataacttct ccggctttta ccggatcacc ttcttttttt gatacttgta
    46441 taactcttcc tgatgccgca gaatacacgg ctgcttcctg ctcgagcatc aggattccgg
    46501 tttcttcaac atattcttct atatctcccc ttgtgacctt tnnnnnnnnn nnnnnnnnnn
    46561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46621 nnnnnnnnnn nnnnnnnnnn nctccggctt cagtatataa ggaagggtca ttatatcaga
    46681 actgaaggat tcaaccatac ccttaatcat acccgcaccc aaagggattc cgactattat
    46741 ggcaatcccg cccattacga aattctccct gcatatcatg ccgtatacat cctttctatc
    46801 aaatcccatg atgcgcaaag ttgcaaattc cattgttctt tcagatattc cgattattgt
    46861 tgaattatat atcagtgcaa atccgagaac tcccccaaac agcatgtaaa accctatggc
    46921 aagcagcatt agatccatgt actcttcaaa agccctgtac atatcatcca cggaatttac
    46981 tgcagagatg ttagcaacat ccttcagttc atccttcaga tcagcctgtg ctgctacaga
    47041 tactcctgta atcagctgtt cctccagaag cagttcttcc attgactcaa tattcatata
    47101 tgcatttgcg cccagatact gcttgaagat gcccgttact ttggtttcaa tatcatcctt
    47161 tcccggtata aagcttttta tggttattgt atctcccttt tttgcattca gcttctccgc
    47221 tatggcttca gtaataaata ttccgctttc cggaagtatt attacattgt ccttcatatc
    47281 tataaactta tggaaggctg tctgccgggg tactcctatg acgctgactg tcatttttct
    47341 ccagtccttt gccaattcaa aggggtattc tatccgcggc tctattctcc ttacttcgat
    47401 caagttgttt aaatcaacta ttattctttc attttgagga ccgttgaact gaattgtgta
    47461 atccattttt tgatattctc cgtattgcag ctcaaacatg ctcatcattg catccttcat
    47521 gtataacgga acagtattaa taccataagc cattgccagc cccagtacaa ggaacgcaaa
    47581 cctcttcttg tttctcaata tgttcctgat aaccatcttc caactgaagg gcacatgctt
    47641 ccaaaagaaa gtaacctttt ctataaaggt acgctttccc gattttggtg cttccggccg
    47701 catagagtca gcaggcatta tgcgtataac ccttcttgcc cccagcaatc ccgatacagt
    47761 gcaaaacata catgtcagca ggatcccgta aaaaatatat ccatattgta tttttactcc
    47821 tgcaagagga atattgaaga aaacagtata atagcggctt atggggcctg ccagcatcat
    47881 cccgccccat atccccaata tagcccctgc caggcctatt gctaaagcgt attttatgta
    47941 atgggaaaga actattctgt tcccgtatcc taaggctttc aatactccta tagccattct
    48001 gtcattattg acaattctgg atagcattat tgaaattatt attgctgcaa ccgtaaggaa
    48061 cataacaggc agcacggaag acaacatttc tattccgtcc agctcctgtt ccaatacatc
    48121 attgctcaac tggtcttcca gttttattat tttttttaca ccatatttgt ccagcttttg
    48181 ttctatgcgg tcaattacat catcaatatc ggcatcggtt tctgtagtaa ccagcacctc
    48241 attatagctt cccctgtaac caaatacgga ttgtgcaaaa gcttcactgg tgtaagctac
    48301 tccgaacttt tcaggagccg gcataagaga ttgttcgttt tccataagat atacgaattc
    48361 cgagcttgat gcaattcctg aaacctttag gctgtgtatt cttccgttga tacgcggata
    48421 aataacatct ccaattttga tattccttgc cttcgcaaac tgctccagca ggattacact
    48481 gctgttgtct tccagtttca tactaacctt ttgttctgag taaagcttgt taatgctgcc
    48541 tccgttatca ggaattgata taagtcgaat tttaaccctt tctttttctt cttccacttc
    48601 caaggctaca tcaacacata cacggccttg tacatcacgg actccttcaa ttgactgcaa
    48661 attctcaagg gcgccctgag gaatttttac cagttgaatt gtcagatcac tgatatttgt
    48721 tattttgtag tagtagtcaa ctgaattcct tagatttgtg cttgttatgc tgaacaatat
    48781 atatatgcat aacgcaacag caacaattac ggtaactgaa acgaactgcc cctttgaatg
    48841 ccttaccatt ctcagcagtc tgacatccag ctttctcatc accattcaat cctttccgga
    48901 ggaatcggag aagcattttc ctttatttct actatgcgtc cgcttctcat ctttattata
    48961 cggtctgcca tatctccaat ggccatgtta tgagtgataa taacaacagt tttttttaat
    49021 tgccggttta ttttatgaag caatgacaat atcaataggc cggttttata atccaatgcg
    49081 ccggtaggtt catcacaaag cagcataagc gggttttttg caacagctct ggcgatagca
    49141 acacgctgct gctctcctcc gcttagttgt gaaggaaaat gatctttcct gtctccaagt
    49201 tctaccgatt ccataacctc gtcaatattc aaggcctcct tgcacatttc tgttgccagc
    49261 tccacatttt cctttgctgt caggttagcc ataagattat agaattggaa tacaaatcct
    49321 accttatccc ttctataggc tgtcagcttc ctgtcattat atttattaat ttcatcgcct
    49381 ctcagaaata ctttgccctg ggttggcaag tccatccctc cgatgatatt cagaagagta
    49441 cttttacctg aaccgctggg cccaagtata gcaatgaatt ctccttcgta aatctcaaag
    49501 cttgcatcac aaagcgcatt aacggtaacc tctcccatct tatagtcctt gcttgaattt
    49561 tctattttca tcaatatctt cttctgcact cttcaccccc tcctttcccc caaacagccc
    49621 gaatttcaaa aaatcaataa aattgccgta aaacttaatc attttgcctt catcgctcgg
    49681 aaagtcaata ttcaaaagtg ccttcggcaa attcatcaac agctcggata taaaacccat
    49741 atccagttcc cgaatctctt cgttcctaat cccttcagaa ataatgaact caaatatggg
    49801 aagcatgaaa ttcttcttga tttccgtcac tttatccaca atgcttttct tctccatgat
    49861 atcccttatg agtacgagtg aataattctt cgccatattt gttgaatagt tataaagtat
    49921 ttcaattctt tcaattgaac tgccgctctc acttattgcc ttcattattt catttgcctg
    49981 atactcaata tctttcctga ttacttccat gaacaaatct tcttttgagt taaaatactt
    50041 atatatagtc attttgctta ttccggcctg atttgcaatt ctgtcaacgg tgatagcgtt
    50101 gtatccgtat ttcagaaata gttctttcga cttatccagt aaattattta actttttttc
    50161 agtttgcctg ttcatttcca ttatcactcc caaattttca gacaagtata cttttatact
    50221 gattcagtat aatattatag tacttcattc cgtgtacttt ttcaattgtc ttgaataaaa
    50281 aaccgttgta aagagaacaa aaaaagtatc accaactact taaaacagtt ttgcgatacc
    50341 ctttttgatt cctatagtta tatatctatc aaattcttct tatagtcatt atacagctct
    50401 tttaattcgc tcattttttc agccatttcc ttttgaagct gtgacagggt ttcatattcc
    50461 ttttcttttg cagcttctct aactctcgtg aagagtgatt ttgaattata ggtgtccttc
    50521 atcagatagt gcctcaggtg atcaacctgc gcccaataag ttatatccag atcctgggct
    50581 ttctgtttgc tgtgaacagc cctgcacttt ctcacagtat ccatgttttc aggaagcagc
    50641 ctgtttatta tctcagttac ccatctgttc atgacggctg tggtaaagct ctttaccagc
    50701 ctttcatcca gaacatttcc ctccagtatc acactgagct tttcaggata ttccgctaaa
    50761 tttttaatat tctcatatac tgttgccgga tgtactccaa ataatctgtt tctttcctcc
    50821 tgggaataat actcgaaaac atcctcctcg cttctgtatg ccctgccttt ttcaaggtag
    50881 aagccttcgg actccggcgc ctttgatatt tccttttcaa gctcggagtt ggttttgcct
    50941 gattcgcagg cagcctttat gccatccagc attgactgat agattgaagc cagcgcaaga
    51001 tatgtgtttg tcttaggatt tggtgatctg acctcaaaac gtgtggcaag cggattactc
    51061 atatttctta ccaatcctgc aagtacagtt ctgttcctgg atggtgtttc aacattatgg
    51121 cccaaggagc agactatgca aaccggtgct tcaaatcccg gcttaagcct gttaaaggca
    51181 tcattggtaa atgaaatgaa aggatttata gcttcatagt tcttcagcag ccccattaag
    51241 gctccgtatc cgataatact taaataatcc tttttcatat ccgacggtgt aaacagattc
    51301 actgttttgc cgttcttaag ccttgcagca actccaatat gagtatgttc gccgtttcct
    51361 gcaacccctt caataggttt tgcgttgaat gtaacatcaa gcccgttttt cctgaatact
    51421 tcttttacaa tgtatctgac ccatttttca ttatctgctg cctggagggc agtatcatat
    51481 ctccagtcta tctccagttg ttccattata tgagtgaggt tgcctgtttc atcaagcttc
    51541 gcctttatgc ctccaacctc cttatgcccc atttcaggct tcagattgta cttatccatc
    51601 aatataagcg actgctccag agaggttctg actattccct ttgttctttt ccaatatgat
    51661 tcctgaagtt cctgggaagc tgtaagggct tccacatctg ccttgtcgtt gggggtcatt
    51721 acccagaatt caagctctgt agctgaagtc aacagtattt catcaatatc gccgcagcta
    51781 attccgtatt ctttgcatac agagggatgt ttttcaagaa tcttcattat ttcagacctg
    51841 aaacgattta ttccacgtat caggattgat cttgagtcta catacttatt gccgtgcatc
    51901 agaaaggaag gaattctcaa agtgccaacc ggcctgcctg tctcataatc aatgttctca
    51961 taattataat ctacgaacca cagggaattg gtatcggcaa ccaaatccaa tttcccgtca
    52021 ttaagtgttg ctatgccggg cagcacaaca cttgatccgt cagtttgaac agaaccgtta
    52081 agaaactctt ctatatcctc gataaaagct cttacaggta ttctttcatc tgtgctgttg
    52141 cctgtcagat ctgcgccggc cagagataca aagcgtattt ccgggtgatc cttcagcagg
    52201 tttataagtt cttccctttg gtactccttt ggattaatca catataatat atcattctta
    52261 atcaaatcac ttagacttgt cattactgcc acctcgtttc aaatatttct tcaataataa
    52321 ttaaaaatta taccaccagg aaagttcaat gtaaataaaa caaaagcgca gtactaataa
    52381 tactttctaa tatagtcatt aatactgctt aactaatacc ttgcaaagcc tgattgccgc
    52441 atgcagcttt atatacttat gcaccctttg cctgccaggt tctgcagagc ataaataagt
    52501 ccgattttta catgttcaaa ggtcactccc ccttgcaaat atgcaatata gggaggcctc
    52561 atgggtgcat ccgcgctcag ctcgatagac gctccctgta caaagcctcc tgcggccatt
    52621 atcacctgat cattgtagcc cggcatatcc caaggctcag gatctacaaa ggaatcaacc
    52681 ggtgaaccct tttggatacc ttggcagaaa gcaattagcg catctctgtt attgaacttg
    52741 atagcttgaa tgatatcact tcttttagta ttgtaatcgg gattgacttc aaaacccagc
    52801 ttcgaaaaca gccttgaaca gaatactgct cctttgactg cttcagatac tatatgagga
    52861 gcgataaaaa gtccttggaa aaaaagcctg ttaatcccaa gggtagcccc gcattctttt
    52921 cctatgcccg gagcgacaag cctgcttgca gccatttcaa ccaaatcagc ccttccgcat
    52981 acatatcctc cggtaggtgc tatacttccc ccgggatttt taatcagtga gccagctata
    53041 atatccgcac ctgcctctgt aggctccttg aaatcgagga attctccgta gcaattgtca
    53101 accaaacaga ttatattcgg attcattatc ttgatgaatt ttatcgtata ttcgatatct
    53161 tccatagtca gggagggcct ccaggaataa cccagggatc tttgtatcag tatcatcttt
    53221 gtcttttcgc ttatcttctt tgttatttca ttataattta tttttcctgc ctcagtaagc
    53281 tctatctgtt tgtacaccac tcccagctcg gtgaggcatc cggcagggcg tttatctttt
    53341 gttccgataa tgttctccaa agtatcataa ggctttcccg taaccgagat gagttcgtca
    53401 ccgggcctta atacaccaaa aagacaaaga gccagtgcat gggtgcccga tactatttgc
    53461 ggccttacaa gggcatcctc acaagcgaat acttccgcgt ataccttctc cagggcttct
    53521 cgccccacat ccccgtagcc gtaaccggta gaacagttaa agtgcatttc gcttatcctg
    53581 ttcctctgca tagcggcaag taccttgtac tgattaaact cacttacctc atcaatcttt
    53641 ttgaattgta ccgagacttc ttcctcggtg gatacgcaga tatctattat cttcttgctt
    53701 atgttcagct tgttaataag ataatctgtt gttttataat gcataatatt acctcttttt
    53761 aatataaatt ttcaatctgt gtattttatt aagcatctaa ctgtatatta cttcagttaa
    53821 aaaaatttgt atgaatcgca attattttat catatttcac agaacaagac aacgcacctt
    53881 aaaattcgga tactataaat ttagtattcc acaaaattcg aaaaatgcga cggggacacg
    53941 gggacgtttc tcccggggac atggggacgt ttcccctgtc ccgctttcag tgggacacgc
    54001 gaaacgtccc cgtgtccccg cgtccccgtg tccccgccgt attattcttc taatatgaaa
    54061 gggatatcga aaactattta ttcaatagtt ttgcgatacc cccttgattg tattgggatc
    54121 aattatttaa ctgtattttc cgtcggtttg ttatcgctga aagcgaaatt aatcggtttt
    54181 acaggtataa tagttgaaat tgcatgctta taaaccagct gctgcttccc ttcgttatcc
    54241 agtactattg tatagttgtc gaagcctctg acatttcctc tgagctgaaa gccatttacc
    54301 agatagattg ttatagtgat gtgctctttc ctgacctgat tcaaaaacaa atcctgaaga
    54361 tttatattta ccttgttcat cctattcccc tccaaaataa aataagcaaa taccaagtat
    54421 atgtatattt atattattat ataattatta gttatttatt ctatattaaa ggaatttttc
    54481 ctgcaataaa cctaattata ttttccacta tgacttcctt tctgaagaaa ttgtcaacat
    54541 ccacccaatt caccctttta tcgcctctga accaggtcag ctgccttttt gcataatgtc
    54601 ttgtatctct tttcattatc ctcactgctt cgtctttcgt aatcaatcca tgcaaaaaat
    54661 ccgcaatctc cttgtagccc aatgcctgca ttgaattaag ctctctgccg tatcctctct
    54721 taataagccc ttccacttct tctaccagtc cggatgccat cattctgtcc accctctgat
    54781 cgattctttc ataaagcttt cgcctgtcca ttgtcaatgt gatgtatgca aagtcatatc
    54841 tgggcggttg tttcctggat ttttcctgaa agtatgaaat cggcctgccc gtatgtttat
    54901 atacttccag ggctcttatc actcttctca ggtcattcgg atgaagcttc ttataagatg
    54961 ccggatcaac agcttcaagc attgcatgca cataatcagt tccttttact tgagctgttt
    55021 cctgcatttc cctcctgtac tcttcatctg aaatggagtc tgtaaaatca agattattaa
    55081 gcagggagtt tatgtaaagc ccggttcctc ccgttattat cggcagcttg ctcctatcat
    55141 tgatatcttt tatatacaat tcagcttttt ctctgaattg ggctacgctg aaatccatat
    55201 caggagtaac ttcatctatc atataatgct taattccctg catttcttct tccttgagct
    55261 ttgccgttcc tatatccata tatttataaa cctgagcaga gtccgctgat attatttcac
    55321 cgcaaaggct tcttgccaga gcaagggata tctcggtttt ccctacggcc gtaggacctg
    55381 cgagtatgca aatttttggc agcaatttaa acacctctga agctatatta ttcttttgaa
    55441 ctttttctcc atctcgtatc tgctcattgt tattatagtc ggccttccgt gagggcaggt
    55501 atatggctgg catgttttca tcagcctctc cagcagcaca cttatttcct ccggagtaat
    55561 ggtgtcccct gcctttatcg aattcttgca tgccatgctt attataatct tttccaaagg
    55621 ggaggatgca ttaggtgccg attccttgag agaatcaagg atattgctga aaaattctcc
    55681 ggtacaaggc tcacccatta atatcggcac tgctcttatt gcaagagtgt tttctccgaa
    55741 ccattcaatt tcaaaaccaa gattctgaaa aataaggcta ttttctgcat acagcacttt
    55801 ctctgcagga cttaattcaa taactatagg catcataagc tcctgcacgg aaatattctt
    55861 ttcattatac ttatctttta tatactcgta cattatcctt tcatgggctg catgctggtc
    55921 tataatgtaa aaatcatcat ccttctgccc cagaatatat gtgccgaata actgtcctat
    55981 tactcttatt tcattgtcga actgtttttg ctgtaccttc gtcttcctgt cataatcgta
    56041 ctgccactta tagctgtcgg cccgcggagc tgcagcctcc cttccgaaag aaagctggtc
    56101 aaaagacact ttttccggct cgtaggagtt tctgctttca aatgcagctg acggcgccgt
    56161 ctgaggttcc gttacatcct caaggttctc aaataccagt ctgttgttat tcagacagct
    56221 ttttaccgcc aggtacagtg tattgaaaat agccctttca tctgagaact taacctcctg
    56281 ctttgtcgga tgcacattaa catccgtcat ttctgatttt atattgatgt taataatgct
    56341 gaaaggatag cggttattca tcagaagggt tttatatgcc tgcttcacag cttctgacat
    56401 cattctgttt tttacatatc ttttatttac agaaaaggtc atataggtag aattgcctct
    56461 tgcaatttca ggctttccta tatatccttc aatgcttata atatcatttg aatattctag
    56521 agggagcatc gctcctgcaa cgctcttccc gtatatgcac agtataaccg aaagcaaatc
    56581 accgtttccg ggggtatgca gaaccacttt gttgtttact gtaagcttca tggatattcc
    56641 ggtatttgtc atggcaagct tttcaataat gtcaactatg gccccctgtt ccgatgtatc
    56701 gcttttcagg aacttcagcc ttgccggggt attataaaac aggttcctta cagtaacgct
    56761 tgtgcctgtt ggatagccga tgcttttact ctgcacaagc ttcccgccct tgataatgac
    56821 atggcttccg gctgcatcct cctccgcctt tgttaaaagc tccacctcag atactgcagc
    56881 tatgcttgcc atagcctctc cgcggaagcc cagggtgcta attccctgaa tatcggcgga
    56941 gtcttttatc ttgctggttg catgcctgaa aaatacttgc tcggtatcct ccttcattat
    57001 tccgcagccg ttgtccgaga catttataaa gctttttccg ccatcctgta tctcgatgct
    57061 gacagctgcc gcccctgcat ctatagaatt ttccaccagt tcctttacta tagaggcggg
    57121 cctttctatc acttctcctg ccgcaatctt atttattgtt gcctcatcca gaatatgaat
    57181 tttccccata atattttctc ctatatcata tctttggcct tatcctgcag ctcctgcagc
    57241 ttgttaagcg cctgaacagg tgtcatgtta accagatcca ctgatttcag ctcttcagcc
    57301 aatgaaaaac tagcaaaatt aaataggtta ggctcatggt cgtttataac cggattcctt
    57361 ctccttcgcc tcacatatac atcagtgtga ctgttctttt cggatttctg aagtatcgat
    57421 attatctccg aggacctttc aagtaccgct tccggtattc ctgcaagccg agccacttga
    57481 attccgtaac tgcctcctgc tccgcccctt gcgattttcc tcagaaatac tatatctttt
    57541 cccttttcct ccaccaggat gcaatagttc ttgacccctt caaccttgtc ctccagttcg
    57601 gtaagctcat ggtagtgggt tgcaaaaaga gtttttgctc ctagccttgc tttgtcagct
    57661 atatattcca ccaccgacca ggcaatgctt agcccgtcga aggtacttgt gcctcttcct
    57721 atttcatcaa gtattatcaa gctcttagag gttgcattat ttatgatatt ggcaagctcg
    57781 ctcatctcca ccatgaaggt gctctggccc gatgccaggt cgtcggaggc ccctactctg
    57841 gtgaatatcc tgtccacaac tccaatctcc gcttttgatg ccggaacgaa gcatcctatc
    57901 tgagccatca gcgttataag tgccacctgc ctcatataag tggatttccc tgccatattg
    57961 ggcccggtga taataagcat cctgttctca tcattatcca actgtgtatc gttgggtacg
    58021 aactttagtt cgttgtcctg ggcttccacc accggatgcc ttccgttttt tataattatt
    58081 ctgtcctcat tatttacttc cggccttaca taattatatt tctgggatac ctcagccagg
    58141 gaacacaggc agtcaagatc ggaaaggcat gaagctgtct gctgtatcct cttgatatgc
    58201 cccgctattt cattccttat ttgtataaag gtctggtatt ccagttctat aagtttttcc
    58261 tccgatccca gcaccaggtc ttcaagcctc ttaagctcag gagtgatata tctttccgag
    58321 tttgagaggg tctgtttcct gataaaatac tccggaaccg aaggcagatt tgattttgtt
    58381 atgtcaatat aatagccgaa taccttgtta aagcctattt ttaaagactt gatacccgtt
    58441 ttttccttct ccgcctgttc cagctccgca atccactgct ttccgttgac cgatgcagaa
    58501 cgtatctcgt ccagttccgg aagataacca tccttaatga gattcccttc cctgagtaca
    58561 aagggcggat cttcattaat tgatgcatca attattccga acaaatcctc aagcacatcc
    58621 aacttcccgt atatatcttt cagaaggatg cttgtacaat cagacagcag gctctttata
    58681 tagggcagat tcctgattga atttttgaag gctatgaggt cctttccgtt ggcacttccc
    58741 aaagctatgc gggttgccag cctttcaaca tcatacatgc ttttcaggta ctccttaatt
    58801 tcttcccttt tcagaaaatc atcatacagc tcagctacag cgtcaagcct gccgttaatt
    58861 tcaaccgtgc tcaaaagtgg cttttccagc cagcttttaa gcttccttcc tcccatggcg
    58921 gtggaagttc tgtctattac tgacagaagt gctcctttcc tggatttgcc cctgattgtc
    58981 tccgaaagct ccagattcct tctggtgctc tggtccagca ccatgaaatt gcttatgctg
    59041 tatgtcttaa tatagtttat attatccaaa ctgacctttt gaatgtcatc aagatataac
    59101 agcaatgccc cggtacttct taatagtgtt tcattgtcta ttccgagccc ttcaaggctt
    59161 gatacaccga actgcctttt gagccttctt tcacaggctt catggtcaaa atatctcttg
    59221 tccaatatgt tataagcaga atatgcagct ttgatatcct gaaaatctgt gttggagatt
    59281 atttcagccg gattaatcct gtttatttcg tttctgacaa ctgccgcatc gcttatatcc
    59341 gcataagtgc agtaaaattc tcctgtggaa aggtctgtat aggacaatgc tgcctgcctg
    59401 ccgtcggcat ataagcttaa caggtaattg ttgactcttt catcaagcat tgagctttct
    59461 attacagtac cgggagttat tatctttatt acctccctgc ggactatgcc ttttgccagt
    59521 gccgggtcct caacctgctc acatattgcc accttgtagt ttttctctat aagccttgca
    59581 atatagctct ctgccgcatg gtacggaatg ccgcacatag gtgccctttc ctcctgtccg
    59641 cagtcccttc ccgtaagggt tatctccaac tccttggatg cggttattgc atcctcaaaa
    59701 aacatttcat agaaatcgcc cagtctgaag aacagtattt tgtccttatg ctggtttttt
    59761 atttcaaaat attgcttcat cattggtgta aactgcgcca ttattttttc acctcatcaa
    59821 ccatttgacc gttcaaaaac catgaatgga tttcatttat ccttacattt accagctttc
    59881 cgattatccc gtcagttcct gtgaaattca caaccttgcc ggttcttgtc cttccggtaa
    59941 attttgaagg gtcattcttg ctgctcccct ctgccagaac ttcaacgatt ttgtccatgt
    60001 attcatcatt cagctttttt gatatgccat tctgaagctc taccagcttt tcaaaattcc
    60061 tgtgtttatc ctcatagccg gacgtatcct ccatttcggc agcagaagta ccgatcctcc
    60121 ttgaatatat aaacataaat acctgatcga attccaccct tctcaataca tcaagggtct
    60181 cttcaaaatc ctcgtcgctt tcaccgggga aaccaactat cacgtctgta ctgagtgcca
    60241 ctccgggtat ttcctgcttt gctttaaata ccttattaag gtattcctct tttgtatact
    60301 tcctattcat tcttttaagt attttgtcgc tccctgcctg aaaaggcagg tgaagctgct
    60361 cgcatacctt tttgcagtcc ctcatggtat aaataagctt atcggaaata tccttagggt
    60421 gtgaggtcat gaaccttatt ctctcaatgc cttctatttc atttaccatt ctaactatat
    60481 cggcaaaatc catttccata ttcaaattct tgccgtagga attcacattc tggcccagaa
    60541 gagtcacttc tttgcacccg tctttggcga ggctttcaat ttctctgatt atgtcccccg
    60601 gcttcctgct cctctccctc cctcttacat gaggcactat gcagtaggaa cagaaattat
    60661 tgcacccata cataattgta acccatgcct tgaacttttc ctcccgcagc acgggtatat
    60721 cctctattat catgccctcg gtgttcatta tatctatcag ggtatatttc tcattaattg
    60781 aacgttcaag aagctccggg agcttgtata ggttgtgagt gccgaatatt atatccacat
    60841 gccggtattt ctttctgatc atttcaactg catgctcctg ctgcatcatg cagccgcata
    60901 cgcctattat cagtgacgga ttgtcttctt taagcttttt taatgcacct atgtttccgt
    60961 acagctttaa ttctgcgttt tctcttacac aacaggtatt gattattatt acatctgcct
    61021 cttcttttat atctgttttg ccatatccca tttctgcaag catacccatt attttttccg
    61081 aatcggagtt gttctgctgg cagccgaagg ttatcacatg catgcgtttc tgctttcccg
    61141 tcttaagctt ttcaatatca ttattctctc ttactctcag ggctatttgc ttttgccgtt
    61201 ctatttcagc attattaact ttctctggtg cttttctttc cgacatatat ttacctcaat
    61261 attt
//
LOCUS       DAOOAB010000010        58147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_645_length_58147_cov_15.212904, whole genome shotgun sequence.
ACCESSION   DAOOAB010000010 DAOOAB010000000
VERSION     DAOOAB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 58147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58147
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_645_length_58147_cov_15.212904"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..683)
                     /locus_tag="PKU88_03760"
     CDS             complement(186..683)
                     /locus_tag="PKU88_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPX68430.1"
                     /translation="MNRNSNIVIGAVLITMGVLFLMSNLGYLNFSWNYIWPLALLVPG
                     IYMHFAFFTGIDKNPGILVPGGILTTYGALFYANVFFGWHIMADLWPLFLIGIAVGLF
                     ELYLFGNRDKGLLIPVGILGGIGLSSLLRVYMRFDIKNYLVPILLIIIGIVIITRKDN
                     RRSNG"
     gene            839..1423
                     /locus_tag="PKU88_03765"
     CDS             839..1423
                     /locus_tag="PKU88_03765"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPX68431.1"
                     /translation="MKDTLRRQVLQARNKLYPEEVLFLSEKVFQNLRKKSFFNNSAHI
                     MVYLDFKNEVKTDFIINHCLENNKKVYVPICIPETHELCISRIINLEELQSGHFGIRE
                     PRPEYIRLSDSSLVDLVLVPGVAFDSTGNRIGFGAGYYDRFMRRLEPGAIKAALAYSF
                     QIADIIPSGQYDIPMDYIVTEKRTISCTASNKPR"
     gene            1434..1961
                     /locus_tag="PKU88_03770"
     CDS             1434..1961
                     /locus_tag="PKU88_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPX68432.1"
                     /translation="MDIFLTFFKIGSFTIGGGYAMLPIIQKEVVDNKKWLGEEEFLDS
                     IAVTNSLPGPLAINCATFVGYKTAGYAGAAAAALGAVLPSFLIILVIAVFFASFRENP
                     IVERVFSGIRPAVVALIAYALFKLVKSMGVNPLNISFSIAVLLLILLFNFHPIVTIVI
                     CGALGFFLYRKEEKQ"
     gene            1958..2488
                     /locus_tag="PKU88_03775"
     CDS             1958..2488
                     /locus_tag="PKU88_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPX68433.1"
                     /translation="MTALLELFLVFLKIGAFSFGGGYAVLALIQKEVIENSGWISPKE
                     FIDIVAIAEMTPGPIAVNSSTFVGYRVGAVAGSAIATLGVVLIPITISLIVSVYYNKF
                     KHLKQVNRIIKGIRPAVLGLIAAACIKIAWASIVDIKGIIIALLTFYGVWKLKINPII
                     AILASGVLGVIFYGVI"
     gene            2640..>2920
                     /locus_tag="PKU88_03780"
     CDS             2640..>2920
                     /locus_tag="PKU88_03780"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPX68434.1"
                     /translation="MAVKVGIPRGMYFYKYYPFIQSFLTELGAEVVPSPETTKEILDK
                     GVLYSIDDACLPVKVLHGHAYSLRDKADCILIPRLTSVSKGEYICPKFGG"
     assembly_gap    2921..3020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3021..3263
                     /locus_tag="PKU88_03785"
     CDS             <3021..3263
                     /locus_tag="PKU88_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68435.1"
                     /translation="SFNGMLALTAFGCGIDSLVGEYIEKQARRYYKKPYMVLTIDEHT
                     GDASFNTRIEAFFDLLDWRNTNEAYISAHGRSIYSS"
     gene            3217..4311
                     /locus_tag="PKU88_03790"
     CDS             3217..4311
                     /locus_tag="PKU88_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HPX68436.1"
                     /translation="MRLTYPHMGEAYIAVKSLMDDIGVESIIPAFTTNSTLRLGSSLS
                     PEAVCLPYKIMLGNYINSINAGADTILITGSCGPCRFGYYGPLQKQALWEAGYKDTDI
                     IILDPPREGYRKFLEALKKVAGKNSGRIIPSFINAYKVCCELDSMNELLYYVRPRAFK
                     KDELRSIMHKFKNDVMAVNGSGQMLELIHDTKNRIRGIDIDKSFKPLKIGIIGEIYTI
                     IEPFSNLHTEERLGDLGVEVHRSMTVRDWARNNVIFPATGRKGYKLEKKLSKPYLPAL
                     IGGHTQQCIGHAVHYAKTGYDGLVQIYPMTCMPEIVSRSILSQVEKDYNIPILYLIVD
                     EQTGEAGFQTRLEAFVDYVNARQEARDTRH"
     gene            complement(4315..5022)
                     /locus_tag="PKU88_03795"
     CDS             complement(4315..5022)
                     /locus_tag="PKU88_03795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPX68437.1"
                     /translation="MLLLAAAVAPSAALLYYFYTRDKYEKEPRKMLVKAFLIGGGLVV
                     PVLFIELSLNIFDIAEANLLAAGYTAFVVAGLVEESSKFLFFILYIWKHRDFNEMYDG
                     IVYSVFISLGFATVENLAYVLSTGFSTAVVRSLTAVPAHALFAVVMGYYLGIARFAKT
                     RYRRKYIILGLIIPVLLHGIYDFILLSQKLYLLVLFIPYMLYLWKRSLGHVDELVESS
                     RFKDRPEEYDDDTEGRV"
     gene            complement(5165..6952)
                     /locus_tag="PKU88_03800"
     CDS             complement(5165..6952)
                     /locus_tag="PKU88_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPX68438.1"
                     /translation="MSIKESILLRSNSIQTKKSRKDRKDKGVFYTPPQVVERITEKLL
                     SGVDLSANPYIKIMDPACGTGLFLIKAFEVLKRKFEENYEVVLDRNEELRGVLKKDGI
                     GSFILENNLWGADIDREALDAAAEMLMKLAGRESKVNLVCCDSLISGSTAQMSFFDDI
                     PPEEYQLWNSSYDYIIGNPPYIGHKQVSGEYKQVLQQLYKGIYKDKSDISYCFIKKGI
                     DLLKDGGSLSFVTSRYFMEGPSAAGLREFIAGHCTVTEVMDFYGDKVFRDAGVAACII
                     TLRKGIFDGETSVLKRRQGIKGNEPELFVPSDFEHFTVKRAELKDEGWVLLSPEKYEV
                     FSMAEAGGSLKLEEIADSYQGIITGCDKAFILNRSEIEENKIEEVLLKPWIKNSNIQK
                     HCIKPADKFLIYSDFIKDEKDFPNAVSFISRCRDRLLNRRECRKGIRKWYQLQWGRTN
                     EVFDTPKIVYPFKSASSRFAVDESGNYCSADVYSLKLKKEYEGRITLEYITAVLNSRL
                     LEFYFKCYAKKVSKDFYDYYPNTVLRMKIPMPAAVNPIGELASKLKNCKNEDARKAVT
                     YEIDREIYRLYGLSKKHIKIIEEDSMIYE"
     gene            complement(6973..8928)
                     /locus_tag="PKU88_03805"
     CDS             complement(6973..8928)
                     /locus_tag="PKU88_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPX68439.1"
                     /translation="MTDMMILHNSRREKYRFPFGAVPCKSTVRLGITVKASQPVNSAE
                     VIVSTECGIEQRIALSAPEGQGEDYYANIEAPEKPGLLWYYFELVIGDKKYYYGNNER
                     FLGGIGGVYGTIPPRYQITVYRREASTPYWYKKAIMYQIFVDRFRNGREDCKVLNPKK
                     NSFIYADWGDTPFYIKDCEKGSIARWDFFGGNLQGVTEKLDYLKELGVSVIYLNPVFE
                     APSNHKYDTADYKNIDPMYGSSETFRDLCEKAGRLGISVILDGVFSHTGSDSIYFNRY
                     GSYPDIGAYQSAESPYYSWYRFNCSKDDYECWWGVDSLPNVNEMDPGYIDFIITGEDS
                     VIKHWMNLGAKGWRLDVADELPDEFIRLIHKTMKEKDKDSVLLGEVWEDASNKVSYGK
                     RRAYLLGEELDSVMNYPFRGIMIDFFTGKRDAEYVNEVLMSIYENYPLHNFYCNMNLI
                     GSHDVPRILTILGEAPPEKQLTQSQKESYRLSESQKALGIARLKLLALIQMTFPGVPG
                     IYYGDEAGLEGYSDPLNRGTYPWGGENRELLEWYKAITSIRNKYDVFSTGRWIPIYSE
                     GDVYGYVRRTEKGRDVFGRLCEDNTALVLVNRSRDFRYPLSLDISQWHRGVIYDILEG
                     GKAADIGEGILDVTLEPLQGKVYIAKL"
     gene            complement(8925..11351)
                     /locus_tag="PKU88_03810"
     CDS             complement(8925..11351)
                     /locus_tag="PKU88_03810"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPX68440.1"
                     /translation="MSVDKNKFKEDYLKKLMTMHAKAFKEATNNERYSALGSLIRDYA
                     AMDWVRTNEEYRRKNAKQVYYFSIEFLLGKLLGNNLVNMGLEKVCEEVLQELGCTLKE
                     VKSAEPDAGLGNGGLGRLAACFLDSMASLGIPGHGCGIRYKYGLFQQKIVDGYQVELP
                     ENWLREGNVWEMRKAGKAVEVRFGGKVAPEYKNNRLEFKHVDYETVLAVPYDTPIIGY
                     NNGTVNTLRLWSAENPEKDFDLSSFSKGDYMKAVEHKYSVESISQVLYPDDSFAEGRK
                     LRLKQQYFFVSAGLQSIVRSYKRTNDNIYDFDEKVAIHINDTHPAIAIPELMRILMDE
                     EGVDWDTAWKITTNTISYTNHTILSEALEKWQIDMFKPMLPRIYMIIEEINERFCGDL
                     WNRYPGQWDKISRMAILADGNVKMAHLAIVGSHSVNGVAKIHTEILKKQELADFYHFY
                     PYKFNNKTNGITHRRWLLKCNPELAQLLTDKIGGSWKKHPTDLIRFLKYRNDPEVQER
                     VRQIKQNNKLELAKIIKDEYSIDIDVNSIFDIQIKRMHAYKRQLLNALHIMYLYNCLR
                     ENPRLDIIPRTFIFGAKAAPGYKLAKQEIKLINTIADKINNDQSIENKLKVVFMEDYK
                     VSLAEKIIPAADISEQISTTTKEASGTGNMKLMMNGAITVATLDGANIEIRDEVGDEN
                     IIIFGMTEKEVLDYYRNGGYSAIEIYNSDPRIRTVADQLINGFFPVSSVEFLDIYNYL
                     LLHNDEYFVLKDFNAYAEAHKRVDETYRNKSEWMKMSIANIAHSGIFSSDRTISEYAS
                     GIWEVKRVIL"
     gene            11567..11929
                     /locus_tag="PKU88_03815"
     CDS             11567..11929
                     /locus_tag="PKU88_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPX68441.1"
                     /translation="MNFRHVTLTVKDLDASIHFYTEIVGLPVDRRYQPGLDTEIAFLG
                     TGETKVELICHRNQPDAIVGNAVSVGFEVEDILEKFESFKGTDISVVSDIIQPNQHVR
                     FFFVADPDGFRVQFLESL"
     gene            complement(11991..13109)
                     /gene="glgD"
                     /locus_tag="PKU88_03820"
     CDS             complement(11991..13109)
                     /gene="glgD"
                     /locus_tag="PKU88_03820"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HPX68442.1"
                     /translation="MLDYMGIINLNEREDNIKGLTGHRPLAAVPIAGRYRMIDFALSN
                     LVNAGVQNISIFTQNKYRSLLDHLGTGKAWDLDRKNDGLFIMNPMYDYYNLGIYRGDI
                     ENFKCHMDYLNFSKQNNVIITPSNMICNLDYWEAIKQHNETGADITIIYKKADNCTVD
                     FQYCNTLEMDEYGRVERIDINFGNENECNVSMDMYIMKKSLFLDIVNSCVSTGYCDYF
                     REAVYKYLHKLKIFGYGFKGYLACINSAYSYYKTSMQLLNMDISKELFFKNGLIYTKV
                     KDEPPTKYKENARVINSLVANGCTIDGQVENSILFRSVKIKKGAVVKDSIIMQNCSIE
                     EESRVINAILDKSVRVSKGKRIIGYADNPEIIEKKAVI"
     gene            complement(13144..14295)
                     /locus_tag="PKU88_03825"
     CDS             complement(13144..14295)
                     /locus_tag="PKU88_03825"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPX68443.1"
                     /translation="MKKKEWIAMILAGGQGSRLTLLTKKIAKPAVYFGGKYRIIDFVL
                     SNCSNSGIDTVGVLTQYQPLVLNSYIGIGSPWDLDRKKGGVTLLPPYVNERGGEWYKG
                     TANAIYQNINFVDEYDPDYVVVLSGDHIYKMDYSKMLDYHKETSAEATIAVIEVPWEE
                     TCRFGIMNTGPDGRIEEFAEKPRNSKSNLASMGIYIFNWDILKKYLVEDEADPESSND
                     FGKNIIPKMLNSGIRLFAYPFDGYWKDVGTIESLWEANMDLLKEDNRLNLFDPKWKIY
                     SVNAIKPPHFAGATADIKCSMVGEGCVIHGEVRNSVLFPGVYIGKDTKVIDTVVMPKV
                     MIGENVVVERAIIGLESVINNGCRIGDSTSGSITLIGEYEQLPQGTVYI"
     gene            complement(14373..16322)
                     /gene="glgB"
                     /locus_tag="PKU88_03830"
     CDS             complement(14373..16322)
                     /gene="glgB"
                     /locus_tag="PKU88_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPX68444.1"
                     /translation="MEGQPHSSYDAYLFREGNHYRSYGFLGAHFTNINGTDGAQFLVW
                     APNADAVHLIGDFNNWRSGANPMVKDDDSGIWSVFVPGLREWDMYKYEMHSRDGRVNA
                     KSDPFAFYSELRPGSASKLVSLGSYKWNDGNWMENRKRTNIYESPVNIYEVHAGSWRQ
                     KPDGRYYSYRELAEELVDYVKNMGFSHIEMMPLTEHPFDGSWGYQSTGYYSVNSRYGS
                     PYDFMYFVDKCHQEGIGVILDWVPGHFCKDDHGLRLFDGTTLYEYEDPKKGENYGWGT
                     CNFDLGKPEVHSFLISNAVFWFDIYHVDGLRVDAVASMLYLDYDREPGEWIPNIFGGR
                     ENLEAVDFVRKLNKVVFEYFPGVMMIAEESTAWPLVTAPTYLGGLGFNYKWNMGWMND
                     MLKYMKTDPSDRKRHHNLLTFSLMYAYSENYVLPLSHDEVVHGKKSLLDKMPGDYWQK
                     FANLRVFLGYMAAHPGKKLLFMGGELGQFIEWRYDHPLDWMLLDYEMHRGIQNYVKML
                     NQVYAREPALWELDHVFEGFEWIDADNSMQSIITFLRKGKRPEDTVVAVCNFTPAVYE
                     GYKIGVPYNCSYYEIFNSDRQEFGGSGFNSTLIFSAVKKEWHNRQQMIKINVPPLAVL
                     YLKPAAIPENERKMETVQLETAAAK"
     gene            16620..17828
                     /locus_tag="PKU88_03835"
     CDS             16620..17828
                     /locus_tag="PKU88_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX68445.1"
                     /translation="MKKSAYKRIALAMLTLIILFITVIPSYADTSVYKRGDSSAEIAE
                     IQAALKKLGFFDEVCTGYFGEVTEGAVLKFQEKYNLGTDGIIGPITIGKLNELTGGHI
                     AIPSRSSSAKKDTTKVNVRYKIKKGDTIWDIAGDYNVSVESILDYNGLNEKSILTIGD
                     EIIIPNVVKVVKPAPVQTVPPPSVQDSSELGNIYEHNKNIYVVKEGDTIESIAEDYGV
                     SAESIKNENNFNNDTVIQIGQEIKIPEGTDQDVEEDTTEASDASGTKGEYLDWFKDVQ
                     YIYDKGDKATVTDVETGKSFNVRRLYGRNHADTEPLTPEDTKIMKSIYGGSWSWNRRP
                     IILTIEDDEGVLRKIAASMNGMPHGGEDITDNGMNGHFCIHFRNSRTHSGSRSDPGHQ
                     KAVKIAAGLE"
     gene            18288..18704
                     /locus_tag="PKU88_03840"
     CDS             18288..18704
                     /locus_tag="PKU88_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPX68446.1"
                     /translation="MIDLIKFLKEKGYKVTPQRIAVYDILKNTKEHPSVDMLYSKLQP
                     VYPTMSLATVYKSLEVFKDLGLVQELNVGEDKFRYDANVNQHPHITCIACGKVEDVYD
                     EMLFDLADQVQKKTGYQLTNQQLYFFGYCPSCKRNH"
     gene            complement(18845..19192)
                     /locus_tag="PKU88_03845"
     CDS             complement(18845..19192)
                     /locus_tag="PKU88_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68447.1"
                     /translation="MFTLRSNEAGLAASGYTYLKYNDGVYVGAEKTESGDVKAEVTIK
                     NAKIKDIKLIEMPPDYISNNPNIKDEISDVIYTIIKKQDVAAASDTGTASYVLSKVIK
                     AVRNALNESMLVQ"
     gene            complement(19422..20399)
                     /locus_tag="PKU88_03850"
     CDS             complement(19422..20399)
                     /locus_tag="PKU88_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPX68448.1"
                     /translation="MMKFYIGIDVGSVSTNVVAVDDSFNVLSKQYIRTNGQPLESVKE
                     GLRNLKAELGSGSKVLGAGVTGSGRQLSGIMVGADVVKNEITAHATAAITYFPDARTI
                     FEIGGQDSKIIMLKNGMVVDFAMNTVCAAGTGSFLEHQAERLKVRIEEFGDLALRAER
                     NVRIAGRCTVFAESDMIAKQQYGFSKADIINGLCEALVRNYINNLGRGKKLEPPFVFQ
                     GGVAANKGIKTAFEKVIGHQVVVPGHYNVMGAIGAAMLAREYILRSGKTTGFRGFEAD
                     DFDFVPASFECSDCDNSCEVIKVEMDGKVAAMWGDRCGKWTNSVNNNPL"
     assembly_gap    20481..20580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20666..22117)
                     /locus_tag="PKU88_03855"
     CDS             complement(20666..22117)
                     /locus_tag="PKU88_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPX68449.1"
                     /translation="MGEVIIPEDIEINIDKSSGSPIYIQLYEQLKKYIIEGIVTEDCK
                     LPTIRDFAEHLGVNSITIVNAYKLLEDNKLVYKKVGSGTYVMPLKAVINLESNFTYSL
                     DEIEFDMDMGADEDIINLSTASPDPKLFPIKDFRRMLDEVLERDGGRAFMYHENMGYK
                     PLRESVSKYLTEYGINAEATDIHVISGAQQGIDIISRVLLTNGDCVFVESPTYNGAVA
                     VFKSRGAKIVEIPLMADGPDMKELEKKLALIKPKLIYVMPNFQNPTGYTYSERKRKYL
                     LLLCKKYDVTVVEDDYMSDLSYLDVGKRPLKALDREGRVIYIKSFSKIFMPGLRVGYL
                     IIPEGLKENVMGIKHITDIYTSGLMQRVLDLYFRSGIWSKHLEYMKREYSLRYFEAVR
                     SARKYLRGASFTQPYGGLNLWVKLPEKVSSVELFERCRDRKVIFTPGTVFIKGEAGEM
                     HIRISFAAASLGKIAEGISIIGNVMEEIKDSAK"
     gene            22271..23326
                     /locus_tag="PKU88_03860"
     CDS             22271..23326
                     /locus_tag="PKU88_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68450.1"
                     /translation="MKIENSNICLKSEHSSVTFKQKQENMQFWIGDNPETSMNSPPGI
                     IIDISKKAMELHQQISSTNSQCIDTDIEEELAGEDKQKLMLIEAMLTQLLGKKFRFKI
                     PKKIRINSDMPINRGVPANQGNGNPRAGWGLIYDSKSIHYESEKVSFSSTGTIRTSDG
                     REIKIDLQLNMSREFFSSEEIHIRAGDALRKDPLVINFDAPSAILSDNKFSFDIDCDG
                     SPDQISRLLAGSGFLSLDLNNDGKINDGSELFGPQSGDGFADLSQYDLDENGWIDEND
                     IIFDRLRIWTKDEYGNDTLFALGEKGIGAIYLGSAGTAFSLRGEENKENGVIQKTGIF
                     LHENGTAGSVQHVDLIV"
     gene            complement(23346..24323)
                     /locus_tag="PKU88_03865"
     CDS             complement(23346..24323)
                     /locus_tag="PKU88_03865"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HPX68451.1"
                     /translation="MKKLIRIFALVLLIMFAVSGCFIKGADLPEDMVGMPSEGYGNTL
                     GNITNGGVVAFQEGWVYYCNGNDDVNIYKMRINGSEKTKLNSDKSMFLNVQGDWVYYS
                     NIEETEKSSIYKVKTDGTKRTKLTNDSASFINVVDEWIYYSNASDNRRIYKIKTDGTE
                     KTLIGDNFSSYLNVHDGWIYYTNGDAKKRIYKVNIEGTENVIVSEDSAAYINVDGDWI
                     YYSNGSEGNKLYKIRTDGTENTELTKEEALFINVSEEWVYYSNGYNGNRIFRIKKDGT
                     DKAILNYESSKFINIADGWLYFIVDSASYRLFKMKSDGTKIQMVEKVER"
     assembly_gap    24401..24500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24646..25272
                     /locus_tag="PKU88_03870"
     CDS             24646..25272
                     /locus_tag="PKU88_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HPX68452.1"
                     /translation="MSVPSFKRLPMHIGVIPDGNRRWALGKGLEKQDGYCHGIKPGFE
                     LYKLCIELGIKELTLYGFTEDNTRRPSIQTQAFRKACVDAVMELTGKDAELLVIGNTS
                     SPMFPQELLPYTKRAKFGRGLIKINFLVNYGWNWDLNHPEHIASADISRVDLIIRWGG
                     RRRLSGFLPIQSVYADFYIVDDLWPDFKPEHLYKALEWYQYQDVTLGG"
     gene            25290..26639
                     /locus_tag="PKU88_03875"
     CDS             25290..26639
                     /locus_tag="PKU88_03875"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPX68453.1"
                     /translation="MKYDIEIPIPRKVQFILDKLHEHNYQAFIVGGCVRDSILGKVPE
                     DWDIATDALPGEVKELFQKTADTGIRHGTVTVIIERSLFEVTTYRIDGEYADFRKPKA
                     VTFTSSIEEDLSRRDFTINAIAYSPEAGIVDPFGGVADIRKGIIRTVGDPERRFSEDA
                     LRMLRAVRFSAQLGFSVDEKTLKSIEINSSLIGCISSQRIRDELTKILLSDNPLCFSL
                     LKDTKLIKYTLPEFEPCFITNQNNPYHSYNVAEHTLRSVAHVEKDKILRWAMLLHDIG
                     KPGTKTTDSKGIDHFYNHQQLSVKLSGTAMSRLRFDKKSMDKILLLVRCHDMHIKADA
                     KSVRKAMSRVGDDMFESLLKVIEADKKAQNPGLLKERTGKFIKIWELYKDIKEKGQCT
                     SLKSLAVNGDDLIALGIKPGKKIKELLNSLLEKVIEEPELNKKEILLSLISQHHRAE"
     gene            complement(26610..27257)
                     /locus_tag="PKU88_03880"
     CDS             complement(26610..27257)
                     /locus_tag="PKU88_03880"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPX68454.1"
                     /translation="MLKKLKLFFIKPTELFRDYIEKPVWALKLFVIALVIGLYTYGTK
                     ILGKDLFIEMMEEKAAAMPPEQAEALRASIAFMNTPMMNLISAVVAAISTVAIILLVA
                     LVYKAFIRALKGEIKYKQVLAVYTLAYMASIVGIAVKFAYMYFTGNLLYLDLSPTFKD
                     VLLNNLDPFVIWQSILMVFGISAVSGIPEKKSIIIVAVMWLVALAISFGSVMLAN"
     gene            complement(27431..27814)
                     /locus_tag="PKU88_03885"
     CDS             complement(27431..27814)
                     /locus_tag="PKU88_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68455.1"
                     /translation="MEIEFHRVLLDALTEAIMRKSVVTVKNMENDSMIVVTATEDEII
                     ISLSRIPSMMNDVISAVSDCLGRCPDMIYDRASVLKDDIVIYYMVWLNNNEGGRSFVE
                     KEFAVPQNRITYLSQKALGYLQNMN"
     gene            complement(27982..28686)
                     /locus_tag="PKU88_03890"
     CDS             complement(27982..28686)
                     /locus_tag="PKU88_03890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPX68456.1"
                     /translation="MEVYEAFIIILTFLLLNVPIFISLYEYIFKDMSLIAKFAVGVVY
                     WSAAFLIHELAPFIGVLILLSKVHRKAEVEIHERNINIWSATAGDIIFVAGATLVFKA
                     VINQINKLYVYILNIYFNMDAKPQEIVEEFAGGEMYYKVMLFILVVILAPFVEEYIFR
                     YYIYDKLLLKSMPHAAAAIISTLIFTLLHFNISGIPTFLGLGLYCTFIYERKGFYGAV
                     TAHLVSNLVTAAMLIV"
     gene            complement(28712..29293)
                     /gene="yihA"
                     /locus_tag="PKU88_03895"
     CDS             complement(28712..29293)
                     /gene="yihA"
                     /locus_tag="PKU88_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPX68457.1"
                     /translation="MIIKKCEFIKSAVHQNQYPELDLPEIAFSGRSNVGKSSLINSLL
                     NRKKLVKTSSSPGKTRLINFFMINDEFVLVDLPGYGYAAVSKSQRQAWGKIIENYLRY
                     RENLKNVVLLVDIRHEPTTDDKLMYDFIKYYRKSVTVVATKLDKITRSSVNKNLNIIK
                     QKLDINQNDILIPYSSETHVGREELWEVLLTWK"
     gene            complement(29283..31625)
                     /gene="lon"
                     /locus_tag="PKU88_03900"
     CDS             complement(29283..31625)
                     /gene="lon"
                     /locus_tag="PKU88_03900"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPX68458.1"
                     /translation="MQKRKEDIKRLPLLPLRGLSIFPYMVLHFDVGREKSIRALEEAM
                     INDQLIFLVTQRDAHTDLPTVKDFYEVGTISKIKQLLKLPGDTIRVLVEGINRAEIRD
                     IISEDAFFLVDVLEHQDVQLSGDLELEALRRSVMEVFDEYIKANPKVAPETLITVSDI
                     DDASRFADVIASNLTIKIEEKQEILSLFDVKERMEKIFEILSRELEILEIERKINSRV
                     RKQIDKSQKEYYLREQLKAIQRELGEKEGHGEEIEQYAEKLSKLEVPDEVKEKVEKEL
                     DRLSKMSQGSPEVGVTRTYLDWIFDLPWNTLTEDNLDIKNARKVLDEDHYGLKKVKER
                     VLEYMAIRQLSNNMKGPILCLVGPPGVGKTSIARSIARAMGRKFARMSLGGVRDEAEI
                     RGHRRTYIGAIPGRIIYSIKQAGSRNPLFLFDEIDKLASDFRGDPASALLEVLDSEQN
                     KEFRDHYLELPFDLSKVMFLTTANSTDTIPPALLDRMEVIYISGYTEEEKLHIAADYL
                     VPKQIKEHSLKDESVSISEAVIRAVINGYTREAGVRNLEREIANIIRKCATQIVENNK
                     KRVRVTPGNLKKYLGIQKYRHDLINQKNEIGVVTGLAWTPVGGDTLFIEVSVMPGTGK
                     LELTGHLGDVMKESARAGLSYIRSRCEEFSIDKEFTKNMDIHIHVPEGATPKDGPSAG
                     ITMTTALVSALAKIPVHRNVAMTGEVTLRGRVLPIGGLKEKVLAAKRAGIKKVILPYE
                     NEKDIEEIPDTVKKNMTFVLVSDMDEVLSHALIRDEKHDN"
     gene            complement(31718..33433)
                     /gene="lonB"
                     /locus_tag="PKU88_03905"
     CDS             complement(31718..33433)
                     /gene="lonB"
                     /locus_tag="PKU88_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HPX68459.1"
                     /translation="MSQLLIAIQLFFAVVIGMYFLNLLRGQQGNKVAIEKESRKEMEK
                     LQHMRDIQLTKPLSEITRPSDFREIIGQENGIKALRAAICSPNPQHVIIYGPPGIGKT
                     AAARLILEEAKKTPNCPFRQDAKFVEVDATTVRFDERGIADPLIGSVHDPIYQGAGAM
                     GVAGIPQPKVGAVTKAHGGILFIDEIGELHPIQMNKLLKVLEDRKVFLESAYYSSEDA
                     NIPFHIHDIFQNGLPADFRLIGATTRGPESIPPALRSRCLEIYFRALTSEEIGIVTSN
                     ALNKIRFECEEGVPELIGKYATNGREAVNIIQTAVGVNINEKRNKVTISDIEWVLNNG
                     QYSPRPERKINDEPQIGYVNGLAVYGPNMGTLMEIEAIAVRAGRGTGRLLVTGIVEEE
                     EIGSRDGRTIKRKSMAKASTDNVITAIKKYLGIDPGEYDIHLNFPGGTPVDGPSAGIT
                     IAVAIYSAITNSYVDNRIAMTGEVSIRGKVKGVGGIIPKIEAAVRAGVKKVIIPKENW
                     QEIFSTYDIEVISVESIVEAVNIATMRQAEVKHLKAQSSEGNILTASKIAKAPSMQAI
                     SDIKQ"
     gene            complement(33577..34872)
                     /gene="clpX"
                     /locus_tag="PKU88_03910"
     CDS             complement(33577..34872)
                     /gene="clpX"
                     /locus_tag="PKU88_03910"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HPX68460.1"
                     /translation="MAKFDDKKQLKCSFCGKNQEQVRRLIAGPGVYICDECIELCQEI
                     IDEEFYEEFDTELQDVPKPAQIKEFLDQYVIGQEEAKKALAVAVYNHYKRINSDYKID
                     DVELQKSNIALIGPTGSGKTLLAQTLAKMLNVPFAIADATSLTEAGYVGEDVENILLK
                     LIQNADYDIERAQKGIVYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVASV
                     PPQGGRKHPHQEFIQIDTTNVLFICGGAFDGLEKIIMNRTGKKSMGFVADIHSRNHQD
                     IGEILKKIMPEDLLKFGLIPEFVGRIPIIVTLNPLDKDALVKILTEPKNALVKQYQKL
                     FELDNVDLEFEDDAIEEIAQKALDRKTGARGLRAIFEENMLNVMFEIPSRDDIDKCIV
                     KRETILEHKEPDIVYLEPGTKKTVKKQPRKGQKARKGTA"
     gene            complement(34887..35471)
                     /gene="clpP"
                     /locus_tag="PKU88_03915"
     CDS             complement(34887..35471)
                     /gene="clpP"
                     /locus_tag="PKU88_03915"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPX68461.1"
                     /translation="MSLVPIVVEQTNRGERSYDIYSRLLKDRIVFLGDEVNDVTASLV
                     VAQLLFLEADDPDKDINFYINSPGGSITAGFAIYDTMQYIKPDVSTICVGLAASMGAF
                     LLAAGAKGKRFCLPNSEVMIHQPSGGAQGKATDVGIYAERLIRSRKKLNEILSAKTGQ
                     PIEKIERDVERDYFMSAAEAKEYGLIDDILVKRK"
     gene            complement(35499..36785)
                     /gene="tig"
                     /locus_tag="PKU88_03920"
     CDS             complement(35499..36785)
                     /gene="tig"
                     /locus_tag="PKU88_03920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPX68462.1"
                     /translation="MNSKLEKLENNVATLEISVSAEKLEEGIMKSYLKNVKKFNIAGF
                     RKGKAPRKIIERHYGEAVFYEDAINIICPDAYDEAVKEHNLEPVDRPDIDIVDIESGK
                     GLVFKAAVTVKPEVALGQYKGIEAEKKEYNVTDEEVDKEIETIREKNARLIDISDRPV
                     KNGDITIIDFKGFVEDKQFAGGTSENYSLEVGSGQFIPGFEEQLVGAELGKEIDVNVQ
                     FPKEYNSEELAGKPALFKVTVKEIKEKQLAELDDEFAKDVSEFDTLDELKEDIRKKKE
                     EQYKRLEKQQYEDEVINKVVENATVDIPEVMVDAQVRIMLRDFDYQLRYQGLNLEAYL
                     KYMNMDIEKLKESYRDTAKSRVKSQLVLEKVAEVEGIAAAEEDLNAEIEKTAKHYNQD
                     LEKFKKTLKEDEITYIKDGIVIQKAIDFLVDNSVAI"
     gene            complement(36931..37848)
                     /locus_tag="PKU88_03925"
     CDS             complement(36931..37848)
                     /locus_tag="PKU88_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPX68463.1"
                     /translation="MKAKRLKTGDTIGIVAPASPSETLKAERAIKHITDMGYSVKAGK
                     SVYSSRGYLAGADELRASDINDMFADDEISAVFCLRGGYGSQRILDLVDFERISSNPK
                     IFMGYSDITALLNAIYQKCGLITFHGPMGGDFAGGLEKRTKRAMKNILESTEPAGELP
                     NPVTPGVVEEGKASGKLAGGNLSIVAASLGTPYEVDTRNRILLLEDVFEEPYSVDRML
                     TQLKLAGKLKSAAGIILGDWGNSEPEEPEKSLSLEEVFEDIFKGLGKPVLKGLKIGHC
                     KPNFTVPIGAEVSIDTCSKTVCILESAVE"
     gene            complement(37892..38257)
                     /locus_tag="PKU88_03930"
     CDS             complement(37892..38257)
                     /locus_tag="PKU88_03930"
                     /inference="COORDINATES: protein motif:HMM:NF022158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HPX68464.1"
                     /translation="MGFTNSFNDFLFSVVPFFMFIIFVIVTMTFITSFTEGLGQYLRN
                     KSAPKKTIPARLAAKRTHDWGGYGDTDIRISYYAAFETENGERLEFPVSSSFSEMYAE
                     GDTGMLTYKGTKFIDFKIE"
     gene            complement(38495..39175)
                     /locus_tag="PKU88_03935"
     CDS             complement(38495..39175)
                     /locus_tag="PKU88_03935"
                     /inference="COORDINATES: protein motif:HMM:NF021120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HPX68465.1"
                     /translation="MRRKNIVISALVLCFALMFSACAARRPLQTPAQPGQDTAGRTGT
                     YTGIGPSTTNPYDTGYGEFPSYYGGYGGYGGYNNGMYGGYGNDYRNMGGSNYSPNVGM
                     NNTRLNTTQAQRLARACEAVPGVDNATCVISGNTCYIGVDTDGDLTGRNIAYGNANDL
                     SAVKRQCAQKVKAANPQIQTVYVSTDTNFFERLRRISDGAGNGNLGDNFRNELNELVR
                     GLTPERLQ"
     gene            complement(39361..41019)
                     /locus_tag="PKU88_03940"
     CDS             complement(39361..41019)
                     /locus_tag="PKU88_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPX68466.1"
                     /translation="MQEMITLTFPDNTKCEYPKGMKLLEIAKDFKQHFNSDIVGAIVN
                     NELKELWVELRQDCSLQFIDRTTVYGNRFYSRSLAFLFIIAAKEVFKDCRVTVEHSLS
                     KGLYCEVHKDKPLAGEDIARIEEKMREFRNMDIPFVKRKMTSTEAIEIFTRTQQFDKV
                     NLIRYRKKKHINIYSCHGHYDYFYGYMVPSTGYLKIFELKANGPGLILRYPERSEPNK
                     LPEFKEQKKLFSIFREFEEWGKILELENISHLNDAVMDGRINDVIRVAEGLHEKKIAQ
                     IADMIVASGNKKLVLIAGPSSSGKTTFAERLSVQLRVNGLKPVAISIDDFFKNRADTP
                     IGEDGEYDFETIDAIDIATFNDVIARLINSEEVEMPVFNFHTGNREWKGRKLKIGKEQ
                     ILVVEGIHGLNEMLTQCIPKEQKFKIYISALTHLSIDDHNRIPTSDLRLIRRIVRDYQ
                     FRSADALSTIKRWDSVRRGEEKYIYPFQEEADVMFNSSLVYELAMLNQIVLPLLEKID
                     NGVHEYIEAKRIKKFLQYFLEADMNAIPSNSILREFIGDSCFFK"
     gene            41336..42178
                     /locus_tag="PKU88_03945"
     CDS             41336..42178
                     /locus_tag="PKU88_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HPX68467.1"
                     /translation="MLNYKIVGNAMQQVVVELGADQTMYSEAGKFLWKTDNIKFETSF
                     NVGDKKGHFEEKSGGGVMGKFMDTAVQVGKRKLAGESAAFQVFNVTHGESGLVTFSQT
                     IPGEIKAINIEDFGVLYVQKDGFLAAENTVDFDIALTKKIGAGFFGGEGFILEKFSDV
                     GTLFIGACGNFIEINPADYGGKVQVDTGALVAFDKNIDYDIEWVGGSVGQVAKNLLFG
                     GEGLFLATLSGNGKVLIQSMNITSLARTLFRNATKSSPEDRSSGKMLGGLGSLLGELG
                     GDRY"
     gene            42467..43189
                     /locus_tag="PKU88_03950"
     CDS             42467..43189
                     /locus_tag="PKU88_03950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPX68468.1"
                     /translation="MTDTILFDLDGTLLPLDMEKFTKIYFHEMGNMFNDMIDPKLLAK
                     YIWAATLDMVGNTEYKTNEEVFMDSFSKLIDGDINKYKERFDYFYDTLFHKTKEAAEI
                     QSLIIESISILKKKNYKLVIATNPLFPRKAIHRRIEWAGLDLKDFEYITSYEDNHFCK
                     PQPEFYKEVLDIIGKRPEQCLMVGNDVQEDLIALKLGIKTFLIKDNMIHRTEEAFTST
                     YEGNYEDFYSFVKELPDVSRSS"
     gene            complement(43173..43967)
                     /locus_tag="PKU88_03955"
     CDS             complement(43173..43967)
                     /locus_tag="PKU88_03955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPX68469.1"
                     /translation="MRKIPEKIYKELEKNIYDGISLPKSAIVLFWTVLCLYFVNMKTS
                     RLYAVFFGILFYASLLVLCLILFKDIKRYLSFPKDNNILPRFIGYKPDIILIYPIFLM
                     FLVGAINSLLIASGIFPEALQKMSAVTSGPIELFAKIIALPLAAFTEELINLLLVSLA
                     FNKMKFPGNIRLIISILLAALTFGMLHAIGWGLKIALLIGISYIPVFLATLYTGNIWI
                     SFLAHFYNNFISLFKAYYGNYHFFIVAAICLIPFIWAINAIVRKSG"
     gene            complement(44095..44170)
                     /locus_tag="PKU88_03960"
     tRNA            complement(44095..44170)
                     /locus_tag="PKU88_03960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44135..44137),aa:Lys,seq:ctt)
     gene            complement(44275..46140)
                     /locus_tag="PKU88_03965"
     CDS             complement(44275..46140)
                     /locus_tag="PKU88_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPX68470.1"
                     /translation="MRESGRAIEKYQPLWGSWKVDELIGQGVDCEIYRVFKEEWGKRY
                     ISTVKFMSFSISKNDISEAQAIGISRAAMPEYFKSLLVNVQNEIELMYKLRGNSNIVT
                     YEDHGIYEKKGNQGWDVLIRMESLQALPDFLIGRKLGRLDVVRLGIDICKALEACGRE
                     NIIHRDIKDSNIFVSPREEFKLGSFNMAKELSKGGRTVLTALNPLYMAPELYKEQSYD
                     FSVDLYSLGIVMYKLLNKGRLPFLPLPPEAITVEDTEKSAARRMSGERLPLPADSEEN
                     LGAVILKACSYDWKDRYKSPAEFRQKLERILKAEAKTTMNNQEIQAEDKESIEISKKN
                     EEEAERTAVAEIAASINNASGGSKRRFFSAAAIIVAAMVLAFMIGYIMNYDALPATLE
                     PACEEIAETAEVIPAPAATAVLTTVPAAEPVKTESRIKTAKKRTEAEKLKGKALEYYE
                     KNEYGKAIEVFSELIKVDASYRGFRQYSDSFLQLAREHNLAGVKQYNEGRLEQAVKEF
                     DNALNMLETMKSNTSNYDMELYSKLKELFAGNKDRMLEKAEKIDACFKLANECNLAGV
                     KYYNEGSFKKAKLEFENAIKHLSEIRLLVSKYSGNSYESLMEIYKGNLSRTEERL"
     gene            46389..46760
                     /locus_tag="PKU88_03970"
     CDS             46389..46760
                     /locus_tag="PKU88_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HPX68471.1"
                     /translation="MSLLSQTIQSGDWKGEKHVPVIHCPEKVTPDTPFEVKVTIGDAI
                     PHPNTFEHHIVWIKVFFQPEGSKFPAEIAHFNFSAHGEGETFTDPAGLTRIKIKKSGI
                     IYALSYCNIHGLWESSKEISL"
     gene            complement(46830..47009)
                     /locus_tag="PKU88_03975"
     CDS             complement(46830..47009)
                     /locus_tag="PKU88_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68472.1"
                     /translation="MAYSLVQDEPKPDDIIVRAEDRTFYVQNDISDNVDFFEIDFHNG
                     WFRKVFVVFPNGESY"
     gene            complement(47266..47718)
                     /locus_tag="PKU88_03980"
     CDS             complement(47266..47718)
                     /locus_tag="PKU88_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPX68473.1"
                     /translation="MFMVPYGRKNRGLTERPHDIFNIDNLFDGFFGDSFMPAFFAGDA
                     QIKVDIKENEKEYIVEADLPGVKKEEINVDLNNDRLTISVTRNEETNIEKENYIRKER
                     RSGSYSRSFYVENVIENQISAKFENGVLSMVLPKRESGVSKNKRIEIQ"
     gene            48049..49248
                     /locus_tag="PKU88_03985"
     CDS             48049..49248
                     /locus_tag="PKU88_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPX68474.1"
                     /translation="MIEYIKNWLALKGMNEGISLILSNIITAIAIVLLSIVINLIVKK
                     FFLKIVKAYAAKSPQKWDDILLKNKFFDQIVNIVPGFIIYGFAPVIPMYQNLIQRIAS
                     AYIVIAVLFVVHKLLNAVDDIYRSSQEERTRPIKGFLQVIEIISIIIGIILIISFLIN
                     RSPWIMLSGIGAATAIFSLIFKDSILGFVAGIQLTANNMVKLGDWIEMPKYDADGDVI
                     EITLHTVKVQNWNKTISMIPTYALISDSFKNWTGMQEFGGRRIKRHINIDLTSIKFCT
                     EEMLERLVKTPYLQEFLSSYNNEHINGKQLTNIDAFRAYLVGYLKNHPGLNKEMTQMV
                     RQRQPTEFGLPIEIYVFFDGTKWADYEVVQSEIFSHVIAMVQEFDLRIYQNPSGYDFR
                     NLGKCES"
     gene            complement(49310..51307)
                     /locus_tag="PKU88_03990"
     CDS             complement(49310..51307)
                     /locus_tag="PKU88_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68475.1"
                     /translation="MINNKSREYIVKKTLLFERAFSMDNYNGDSKIDYGYKYEKGTIP
                     VLVSAPHSVNHLRNGKVKCADIFTGSIARMIHLLTGCHVFYKTKNDNYDPNYDPINGD
                     GDYKNSVIDVIRKENIRIFIDLHGAAENREFDIDLGTDGSNNLCGNNFIPDLFRVVFG
                     KYGIHKVEENKMFTASPQNNVSRTMAAVCGIPSIQVEVNRKYREVSVNEEEFMSFMKG
                     LYSVISVLNDCEWDSDTYVFEAEKSKIHIPIDKIEFSREDAEKYGFQRNDSFQVNSVF
                     NHCSKTEFIARYKHTGNNQEFVPGKVYLTNKLYKNVFGDNEAEERKYVLVNRKKAITL
                     PIGIPKAGSERIMICPDLADKIDLSKNYQLYNRHDDIDYCQGKLSVTRDTTAKGKIFL
                     NYYQRHIMNVNIPKKVILRNDFLKYLGSDVLTEAAKDILKRSYKDRHSYYEIEQNLIE
                     DDDLKSIFKKLELDKIELVEVNDTGDKTKAMGLGGRWRRFIYGILSFLIKRKSICLRV
                     GRPYPTDENSDIVRIMPTTMKILGIGETDKLIVRHRGHEAILRALPFDSFEVLKNSNV
                     LVYDDADASTLIGIPSKYRVELGIYSLNSIVSAERDMKYLFFKNSNVQLLPIIAVIFT
                     IIQTFQDTATRILLSCILVPLAIYVSLSQERLKVDSDSKRK"
     gene            complement(51333..52694)
                     /locus_tag="PKU88_03995"
     CDS             complement(51333..52694)
                     /locus_tag="PKU88_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPX68476.1"
                     /translation="MIGVVAIFTNAKEAEDFVYSSYMNAIDKIPKDMPDMVTRNPHLT
                     RALLDKTEEPDKKQRNILVTGSKGKGSVSRMISKLIEAHGLKAGLCTSPHLVSFLERI
                     RINGNAISEGDFVKYANILEPYAAEVQKQLTEAQYIGPAGITAVIAMLYYLDQKTDYN
                     VFECGKGARYDDVTRIYSEASVINTIFPEHIPQLGYNLPEIAFNKAGIINSIQRFAVS
                     AVQQKEVRDVICREACDKGVRLFTFGQDFLCEKICVDRNGTYFDIITRNNKYRNIRLN
                     LMGRHQAYNAAIAVCTAENLLGGVKEENIRQAFDGITWPGRLEVINRSPLTILDGCIN
                     RECAIHVREFVSEMGNKDVISIVGIPDDKDYEGVACEMYDISRRIIFTASKNKHLKFT
                     TAQMDKAKPMLGEKLIYEAEIEDAIETAYNLIGDDGLVCIIGTQSLVRDVKEYFKQDT
                     LNL"
     gene            52968..53480
                     /locus_tag="PKU88_04000"
     CDS             52968..53480
                     /locus_tag="PKU88_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68477.1"
                     /translation="MFNKRILFFCTVLALLAAALQAVPLIFGAAFAFGALLNGFPIYL
                     AARLSYIPGILVYLSAAALLAFLNPFEALFFICTNGIIGLSLGIIKDRFRSIYPVPAL
                     SALLDVAILFTVNYRFGISIFSPSAFSTPAGQAAALLFPMYLYCLIYLKLAMSLDKLI
                     HRYIELDIHP"
     gene            complement(53484..54434)
                     /locus_tag="PKU88_04005"
     CDS             complement(53484..54434)
                     /locus_tag="PKU88_04005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX68478.1"
                     /translation="MGRYFIKALIFCILFFSVCGVIGLQEDFFVPEKAEPVTEPEPEP
                     VPEPVPEIVFSETELLPGDCFAIYLKGLTERDDIQVSTAFVQGQPRFFSFMEGRLAII
                     GTSCRTKEGDYSCKVQVLRDGEIAAEKEETFKVFHKEFVEQHLTVTSTQKEQRSDDNF
                     DMDKVHTERAKSVTSEKPLWEGTFVKPVEGRVSTEFGMIRYINKEESGRHSGIDIAAA
                     RGTPVKAANNGVVRLSMMLNVTGNTIIIDHGCNIYSSYAHLDKLLVEEGAEVKKGDII
                     GEVGSTGFSTGPHLHWSTTIGTLYINPESLTESDPLAFIR"
     gene            complement(54514..54948)
                     /locus_tag="PKU88_04010"
     CDS             complement(54514..54948)
                     /locus_tag="PKU88_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPX68479.1"
                     /translation="MDMMQLVLFRLNEQEYAIDIFKVNEIVPYRKPTMIPNTPDYVEG
                     VLNLRGNVIPVISLKKRFQLEDKSTEENTKIIVADTGGTIAGFVVDEASNVITVEREK
                     IEPVSEVVSNIGSKYINGVGKKDDGSMYILLNFDGLYAEGVL"
     gene            55243..56736
                     /gene="thrC"
                     /locus_tag="PKU88_04015"
     CDS             55243..56736
                     /gene="thrC"
                     /locus_tag="PKU88_04015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPX68480.1"
                     /translation="MNKIMYKSTRGRGRLITASAAILKGIAEDGGLFVPTEIPRPEIS
                     LEKMINMDYKSLAYHIMKDYFQDFEEAEIRECIDKAYDEKFDTPEIAPVVKKSGVYFL
                     ELFHGPTLAFKDMALSILPYFLKTAAKKQKLDSEIVILTATSGDTGKAALEGFAGVPG
                     TKIIVFFPESGVSEVQKLQMVTQTGENTYVIGIRGNFDDAQTGVKEMFSDKELQMDMS
                     AANFVFSSANSINIGRLVPQIVYYFYAYLQLCRMGDIKAGEEINFTVPTGNFGNILAG
                     YHAKKMGLPVRHLICASNENKVLYDFLNTGVYDKKRNFITTMSPSMDILVSSNLERLL
                     FYLYGEDSSMVGELMAQLSGKGAYMINDNVKDKLNDFYGYYASEADTAEAIRKMYEDC
                     GYVIDTHTAVALSAYGKHRKRKCDENIKTVIVSTASPYKFTSDVMKSIDPKYSGQNSF
                     QLIKEMSRLTGAEIPKGIRDLDSRPVIHNTVCNKDEMKKQAEKILGL"
     gene            complement(56737..57438)
                     /locus_tag="PKU88_04020"
     CDS             complement(56737..57438)
                     /locus_tag="PKU88_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68481.1"
                     /translation="MNENAKRILVVDDEEKIAEVVESYLTKSGYKVYTAYNGSNALEL
                     FERINPDLIILDLMLPDMSGEDICRSIRKKSRVPIIMLTARVEEESILTGLGIGADDY
                     VTKPFSLRQLTARVMALLRRSGDDIIPLSDVLSYNDGELLIDSIRHEVKRGGKPVNLT
                     PNEYKILMALVKYPQKAFTRDELVFLVLGEDYEGYDRTIDTHVKNLRQKIEPDAKSPK
                     YILTVYGVGYKFGGE"
     gene            complement(57476..58087)
                     /locus_tag="PKU88_04025"
     CDS             complement(57476..58087)
                     /locus_tag="PKU88_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPX68482.1"
                     /translation="MDILSIVLIAVGLSMDAFAVSVTNGIIIRDLRIGHALKVGLYFG
                     VFQALMPLIGWIAGSQFKEYIVSIDHWIAFGLLAFIGGKMIREACGEDKEEGVEGMCE
                     VAVSNQDTACENPLSMGRLIVLAVATSIDALAVGISFAFLRVSIVQSAVLIGAITFAI
                     CFAGVYIGHKCGCLMKKKAEKVGGFILICIGLKILFEHLGILG"
BASE COUNT        15817 a        13603 c        10716 g        17711 t
ORIGIN      
        1 ttgctcatca tacataaaac aaaaactgaa tataatgcat cggattaaac ataagtaaaa
       61 ccctggcatt atattcagca gcctttattg gttatgaaag agtatatatg taaacagccg
      121 gcacttataa acgcaacatg gcttccaact gcccgaagct ctattggttt tggcctgctg
      181 gttttttaac cgttgcttct cctgttgtcc tttcttgtaa ttataactat tccgattatt
      241 atcagtaaaa tgggaaccag ataattctta atgtcaaatc tcatatatac cctcaataat
      301 gatgaaagtc ctattcctcc aaggataccc accggtataa gcagcccctt gtcacggttt
      361 ccgaaaaggt acagctcaaa aagccccact gcaatgccta tcagaaataa cggccaaaga
      421 tcagccataa tgtgccatcc gaaaaaaaca ttggcataga acagcgctcc atacgtagtc
      481 agtatgcccc ccggtacaag tatgccgggg ttcttgtcaa tacccgtaaa aaatgcaaaa
      541 tgcatatata ttccgggaac cagcagtgcc aatggccata tatagttcca tgaaaaattc
      601 agatagccaa gatttgacat gagaaacaat actcccattg ttattaaaac cgctccgatt
      661 actatattag aatttctatt cataatattc ctcctcaact gcgcttccat aattaccttc
      721 taactaatat acggaggcct caggcaaatg tctgatggaa attcatattt acctctgtta
      781 tattaataat agtactataa ttactatgaa aatccctaag atacatatgg aggaacacat
      841 gaaggatact ttaaggagac aagtgctgca agccaggaac aaattatacc cggaagaggt
      901 attatttctc agcgaaaaag tcttccaaaa tttaaggaaa aaaagttttt tcaacaattc
      961 agcccacata atggtatatc ttgattttaa gaatgaggta aagactgatt ttataataaa
     1021 tcattgcctt gagaataata agaaagtata tgtgcctatt tgcataccgg aaactcatga
     1081 actctgcatt tcaaggatta taaaccttga ggagcttcaa tcgggccatt tcggcatacg
     1141 ggagccaagg ccggagtaca tcaggctgtc agacagcagc cttgtggatc tggtactcgt
     1201 tccgggagta gcctttgaca gtaccggaaa cagaataggc ttcggggcag gctattatga
     1261 caggtttatg agacgccttg aaccgggggc cattaaggct gctcttgctt attcctttca
     1321 gattgcggat attattcctt caggccaata cgacatcccc atggattata tagtaactga
     1381 gaaacgcacc ataagctgca ctgccagcaa caaaccgagg tgatactatg ctgatggata
     1441 ttttcctgac atttttcaaa ataggctcct ttacaatagg aggaggctat gcaatgcttc
     1501 ccattattca aaaagaagtt gtcgacaaca aaaaatggct tggtgaggaa gaatttctgg
     1561 attccattgc agtaaccaac agccttccgg gccctttagc cataaactgc gctacctttg
     1621 tcggctacaa aacagcaggc tatgcaggag cggctgcagc cgcccttgga gcagtcctgc
     1681 cctccttcct gataatactt gtaatagctg tgttttttgc atctttcagg gaaaacccga
     1741 ttgtcgagcg tgtattttca gggataaggc cggctgtagt tgcgcttatt gcttatgctt
     1801 tgttcaagct tgttaaatca atgggtgtaa atcccttaaa catatccttc tccattgcgg
     1861 tgctgctgct tattttactt ttcaattttc atcctatagt aactatagta atatgcggcg
     1921 ccctcggatt tttcctctat agaaaggagg aaaaacaatg actgcattat tggagctttt
     1981 tctggtcttc ctgaaaatcg gggccttcag cttcggggga ggatatgcag tgcttgccct
     2041 gatacagaaa gaagtaatcg agaacagcgg ctggatttca ccgaaggaat ttattgatat
     2101 agttgccata gcggaaatga ctccggggcc gattgccgtt aattcatcaa cctttgtggg
     2161 ctacagggtc ggtgctgttg cagggtcagc cattgccacc ctcggagtag ttctgatccc
     2221 cataacaatt tcactgattg tgtcggttta ctataataaa ttcaaacacc tgaagcaggt
     2281 caaccggatc attaaaggaa tacggcccgc agtattgggg cttattgccg ctgcctgcat
     2341 aaaaatcgca tgggcttcaa ttgtcgatat caagggaatt ataatcgctt tgctgacttt
     2401 ttatggagtc tggaagctta agataaatcc gattattgct atacttgcat caggagttct
     2461 cggagttata ttctacgggg ttatttagat aaaagcatca aactgcatcc aaaacccggg
     2521 atcatgtcat taagctttcc ggttttttat ttttgtttta aacctctgtt cttttgtcaa
     2581 taatatatat gccgtgaaaa aaaattatca cgatatactt catggaaaga ggtgattcag
     2641 tggctgtcaa agtaggcatt ccccggggaa tgtattttta taagtattat cctttcatac
     2701 agtccttcct taccgaactg ggggcggaag ttgtaccgtc tcccgagacc acaaaggaaa
     2761 tacttgataa gggcgtgctg tacagtatag acgatgcgtg ccttcccgtc aaggtactcc
     2821 acggtcatgc atacagcctt agagataagg cggattgcat cctgatacca cgacttacaa
     2881 gcgtatcaaa gggtgaatat atatgcccca aattcggagg nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn tctttcaatg gaatgcttgc gctgaccgcc ttcggctgcg
     3061 gaatagattc tctggtcggg gagtatattg aaaagcaggc acgaagatat tataagaaac
     3121 catacatggt gttgactatt gatgagcata caggggatgc cagcttcaat acaaggatag
     3181 aggctttttt tgatttgttg gattggagga atacaaatga ggcttacata tccgcacatg
     3241 ggagaagcat atatagcagt taagagtctt atggacgata taggagtgga aagcatcata
     3301 ccggcattca ctaccaattc caccttgagg cttggctctt ctttatcacc tgaggcagta
     3361 tgcctgccgt acaagataat gctcggaaat tacataaaca gcatcaatgc cggagctgat
     3421 accattctta taaccggaag ctgcgggcct tgcaggtttg gctactatgg ccccttgcag
     3481 aagcaagcat tatgggaggc aggttataaa gatacagata ttataatact tgaccccccc
     3541 agggaagggt atcggaaatt tcttgaagca ttaaaaaaag ttgcgggaaa aaacagcgga
     3601 agaatcatac cgtcttttat caatgcatac aaggtatgct gcgaattgga cagtatgaat
     3661 gaattgcttt attatgtcag gccgcgagcc ttcaagaaag atgaattgcg aagcatcatg
     3721 cataagttca aaaacgatgt gatggctgta aatggatcag gccaaatgct ggagttgata
     3781 catgacacca aaaaccgaat aagaggaatt gatattgaca aaagcttcaa gcctttaaaa
     3841 ataggaatta tcggagaaat atatactata atcgagcctt tttctaatct ccatacggag
     3901 gaaaggctgg gagatctggg cgtagaggta cacagatcaa tgacggtaag ggattgggca
     3961 cgcaataatg tcatatttcc cgccacggga aggaagggat ataagctgga aaagaaattg
     4021 tcaaagccct acctgcctgc tcttatagga gggcataccc agcaatgcat cggtcatgcg
     4081 gtgcattatg caaaaacagg ctatgacgga cttgtacaaa tttatccaat gacctgtatg
     4141 ccggaaatag tatcccgttc gattctctcg caggtggaaa aggattataa tatacccata
     4201 ctgtacctta ttgttgatga gcagacaggg gaagcaggct tccaaaccag actggaggct
     4261 tttgtggact atgtaaatgc caggcaagag gcaagagaca cgagacacta gacactagac
     4321 acggccttca gtgtcatcgt catattcttc cggcctatcc ttaaaccgtg aggactccac
     4381 cagttcatcc acatgcccga ggcttctttt ccagagatac agcatgtacg gtatgaaaag
     4441 caccagcaaa tacaacttct gagaaagcaa tataaaatca tatatcccat gaagcaatac
     4501 gggaataata agtcccagta tgatatactt ccgtctgtac cgggtctttg caaacctggc
     4561 aatgcccagg taatatccca ttactacggc aaacagggca tgagccggta cggcagtcag
     4621 agatctcaca actgccgtac taaaaccggt ggaaagcaca tatgcaaggt tttcgacggt
     4681 agcaaagcca agggaaatga acacggagta tactattcca tcgtacattt cgttgaaatc
     4741 cctatgtttc catatgtata agataaagaa cagaaatttg gaagattcct ccacaagccc
     4801 cgccaccaca aaagcagtat aaccggcggc aagaaggttg gcctccgcta tatcaaatat
     4861 attcagtgac aattctataa aaagtaccgg aacaacgagc cctccgccta tcagaaacgc
     4921 tttcacaagc atttttctgg gttccttctc atatttatcc cttgtataaa agtagtataa
     4981 taatgcggcg gacggggcaa cggcggcagc cagcaaaagc ataaaactcc tccttagaag
     5041 aataatgcga cgcggggaca tggggacgtt tctcccgggg acatggggac gtttctcccg
     5101 gggacatggg gacgtttctc ccggggacat ggggacgttt cgcgtgtccc tcagacacgt
     5161 cgcattactc atatatcatg ctatcttcct ctataatttt tatatgtttt ttgcttaatc
     5221 catataacct atatatttcc ctatcaattt cataagtaac agcctttctt gcatcctcat
     5281 tcttacaatt tttgagcttt gatgccaatt cccctatagg attgaccgct gccggcatgg
     5341 ggattttcat cctgagaacg gtattcggat aataatcata gaaatcttta ctgacttttt
     5401 ttgcataaca cttgaaataa aattccaaga gccttgaatt aagtacggct gtaatatatt
     5461 ccaaggttat ccttccttca tattcctttt ttagtttaag gctgtataca tcggcactgc
     5521 agtaattgcc ggattcatca acggcaaaac ggctgcttgc agatttaaaa ggatatacta
     5581 ttttgggtgt gtcgaaaacc tcattggtcc tgccccactg cagctgatac cacttcctta
     5641 tccccttcct gcattccctt ctgttcagca gtctgtcccg acaccttgaa atgaagctta
     5701 cggcattggg aaaatccttt tcatccttaa taaaatccga ataaataaga aacttatcag
     5761 caggcttgat acaatgcttc tgaatattgc tgttcttaat ccacggcttt aacagtactt
     5821 cctctatctt attctcttct atctcactcc tgttaaggat gaatgccttg tcacagccgg
     5881 tgataatacc ctggtaactg tctgcaatct cttccagttt caggctcccc cccgcttctg
     5941 ccatagaaaa tacttcatat ttttcagggc tcagcagcac ccagccctca tcctttaatt
     6001 ccgccctttt gaccgtaaaa tgctcaaagt cggacgggac aaacaattca ggctcattgc
     6061 cctttatacc ctgccttcgc ttcaggactg aagtctcccc atcaaatatt cccttcctca
     6121 gggtgataat acatgctgca acccccgcat cgcggaaaac cttatcccca tagaaatcca
     6181 tcacctctgt aactgtacaa tggccggcaa tgaattccct caacccggct gccgaaggcc
     6241 cttccatgaa gtatctggaa gtaacgaagc ttaagcttcc cccatccttt aacaggtcta
     6301 tgcccttttt tatgaagcaa taggaaatat cggacttatc cttgtatatg cctttataca
     6361 gctgctgaag cacttgctta tactcccccg atacctgctt atgtcctatg tagggagggt
     6421 tccctattat gtaatcataa ctgctgttcc ataattggta ttcctccggg ggtatatcat
     6481 caaaaaagct catttgagcc gtacttccgc ttataaggct gtcgcagcac accagattca
     6541 ccttggactc cctgcctgcc agcttcatca gcatttcagc cgctgcatcc aaggcttccc
     6601 ggtcgatatc ggcaccccaa aggttgtttt ccaaaatgaa gctgccgatt ccgtcctttt
     6661 tcagcacacc tctcagttcc tcattcctgt ccaatacaac ctcgtagttt tcctcaaact
     6721 ttctcttcag cacctcaaag gcctttataa ggaaaagccc tgtgccgcag gcgggatcca
     6781 taatttttat ataagggttt gccgaaaggt caacccccga cagaagcttc tcggtaattc
     6841 tctccactac ctgcggggga gtatagaaca ctcccttgtc ctttctgtct tttcggcttt
     6901 tttttgtttg tatgctgttg ctcctcagga gtattgattc ctttatgctc atgcaatcaa
     6961 acctcttgtt cgctatagtt tcgcaatgta taccttcccc tgaaggggtt ccagggtaac
     7021 atccagtatt ccttctccaa tatctgcagc cttgccgcct tcaagaatgt cataaatgac
     7081 acccctgtgc cattgactaa tatccaggga cagaggatac cggaagtccc tgcttctgtt
     7141 taccagcacc agtgcggtat tgtcctcaca cagtctgccg aatacatccc tgcccttttc
     7201 cgtccttctg acatatccgt atacatcccc ttcggaatat atgggaatcc atcttccggt
     7261 gctgaaaaca tcatacttgt tcctgataga ggttatggct ttataccatt caagcagttc
     7321 gcggttttca ccgccccatg gataggttcc cctgtttaaa gggtcactgt acccctccag
     7381 tcctgcttca tcgccgtaat aaatgcccgg aacccccggg aaagtcatct gaataagagc
     7441 cagaagctta agcctcgcta ttcccaaagc tttttggctt tccgacagtc tgtagctttc
     7501 tttttgagat tgtgtcaatt gcttttccgg aggcgcttcc cccagtattg tgagtattct
     7561 cggaacgtca tgagagccta ttaaattcat attgcagtaa aagttatgca aagggtagtt
     7621 ctcatagatg ctcatgagca cttcatttac atattctgca tcccgcttcc cggtaaaaaa
     7681 atctatcatt atccctctga aggggtaatt cataacagaa tccaattcct cccccaaaag
     7741 gtatgccctt ctctttccgt agctcacctt gttggatgcg tcctcccata cttcccccag
     7801 aagcaccgaa tccttatctt tctctttcat tgttttatga atcaatcgga taaactcatc
     7861 cggaagctca tccgccacat ccaggcgcca gccctttgcc cccaggttca tccaatgctt
     7921 gatgacactg tcctcacctg tgataataaa gtcgatatac cccggatcca tttcattgac
     7981 gttgggaagg ctgtccacac cccaccagca ctcataatca tccttggagc aattgaagcg
     8041 gtaccatgaa taatatggag attcggctga ctgatatgcc ccgatgtccg gatagctgcc
     8101 gtacctgttg aaataaatgc tgtcactgcc ggtatggctg aaaaccccgt ccaaaataac
     8161 ggaaattcca agccttcctg ccttttcgca aaggtccctg aaggtctcgc tgctgccgta
     8221 catagggtcg atattcttgt aatcggcggt gtcatacttg tgattgctgg gtgcctcaaa
     8281 aacagggttc agatatataa cagacacacc aagctccttc agataatcca gcttttctgt
     8341 cactccctga agattcccgc caaaaaaatc ccacctggca atacttccct tctcacagtc
     8401 ctttatatag aaaggagtat cgccccagtc tgcataaata aagctgtttt tcttggggtt
     8461 cagcaccttg cagtcttcac ggccgtttct gaacctatcc acaaatatct gatacattat
     8521 ggcctttttg taccaataag gagtacttgc ttctcttctg taaacggtaa tctgataccg
     8581 gggcggaatt gtaccgtata cacctccaat gccccccaga aatctttcgt tatttccgta
     8641 ataatacttt ttatccccta taaccagttc aaaataatac cataaaagcc ccggcttctc
     8701 aggtgcctca atatttgcat aatagtcctc tccctgccct tccggcgcac ttagggctat
     8761 cctttgttca atgccgcatt ctgtgcttac tataacttca gctgaattca ccggctgcga
     8821 ggccttcaca gtaatgccga ggcgaaccgt gcttttgcag ggaactgcac caaagggaaa
     8881 gcgatatttc tcccggcggg agttgtgcag aatcatcata tccgtcatag tatcaccctt
     8941 ttgacctccc atatgcccga ggcatattcg gatattgtcc tgtcgctgga aaaaatccca
     9001 gaatgagcaa tatttgcaat actcatcttc atccattcgg atttattcct gtaggtttca
     9061 tccacccttt tatgggcttc cgcataagca ttgaagtcct tcagcacgaa gtattcatcg
     9121 ttatgcaaaa gaagataatt gtagatatcc agaaactcaa cgctgctcac cgggaaaaag
     9181 ccgtttataa gctggtctgc aacagtcctg atgcgcggat cgctgttgta aatctcaatt
     9241 gcgctgtacc cgccgttcct gtaatagtcc aaaacttcct tctcagtcat tccgaaaatt
     9301 attatgtttt cgtcccctac ttcatccctt atctcgatat ttgctccgtc caaagtagca
     9361 acagtaattg cgccgttcat cataagcttc atatttcccg taccggaggc ttctttcgtt
     9421 gtagtagaaa tctgctcgct tatatcggca gcaggtatta tcttttccgc cagagatacc
     9481 ttgtagtcct ccataaatac aaccttgagc ttattttcta tgctttggtc attgtttatc
     9541 ttatcggcaa tggtattgat aagcttgatc tcctgctttg ccagcttgta gcccggtgca
     9601 gctttggcgc cgaatatgaa ggttctcggt ataatatcaa gcctgggatt ctccctcagg
     9661 caattgtaca ggtacattat atgaagcgca ttcagcagct gccgcttgta tgcatgcatc
     9721 ctttttatct gtatatcaaa tattgagttt acatctatat ctatggagta ttcgtccttg
     9781 ataatttttg ccagctcaag cttgttgttc tgctttatct gtcttactct ttcctgcacc
     9841 tccgggtcat tccgatactt caggaatctt ataaggtctg tgggatgctt tttccaggag
     9901 cctccgattt tatccgtgag aagctgcgca agctccggat tgcattttaa gagccacctt
     9961 ctgtgggtga ttccgttggt tttgttgttg aatttataag gataaaagtg gtaaaagtcc
    10021 gcaagctcct gtttttttaa tatttcagtg tgaatcttgg caacaccgtt gacactgtgg
    10081 cttcccacta ttgcaaggtg ggccattttc acattcccat cagccagaat ggccattctc
    10141 gatatcttgt cccattgccc gggatatctg ttccaaagat ccccgcagaa cctctcgttt
    10201 atttcctcaa taatcatgta aatcctcggc agcatgggct taaacatgtc tatctgccat
    10261 ttttccaggg cttcggacag aatggtgtga tttgtgtagg aaattgtatt ggtagtaatc
    10321 ttccacgccg tatcccaatc gacaccttcc tcatccatga ggatcctcat aagctcagga
    10381 atggcaatgg ccggatgcgt atcattaatg tgaattgcca ccttctcatc aaaatcataa
    10441 atattgtcat tagtacgttt atatgacctt acaatgctct gaaggccggc cgatacgaaa
    10501 aagtactgct gtttaagcct gagcttcctg ccctcggcga agctgtcatc cggatacaaa
    10561 acctgagata tagattccac ggaatatttg tgctctaccg ccttcatata atctcccttg
    10621 ctgaaggagg acaaatcaaa atccttttcg ggattctccg cgctccaaag ccttagggta
    10681 ttgactgtcc cattattata tccgattata ggagtgtcat aaggaacggc aagtactgtc
    10741 tcataatcca catgcttaaa ctccagccta ttattcttat actcgggggc aaccttgcct
    10801 ccgaacctta cttccacagc ctttcccgcc ttcctcatct cccagacatt cccttccctg
    10861 agccaattct caggcagctc cacctggtag ccgtcaacaa tcttctgctg aaaaaggccg
    10921 tacttgtatc ttattccgca gccgtgcccc ggtatcccaa gtgaagccat ggagtccagg
    10981 aaacaagcgg caagccttcc caagcctccg ttacccagcc ccgcgtcagg ctcggcagat
    11041 tttacctcct tcagggtgca gcccagctct tgaagcactt cctcacacac cttttccagt
    11101 cccatattca ccaggttgtt gcccagaagc tttcccagca ggaattcaat tgaaaaataa
    11161 tatacctgct ttgcattctt tctgcggtat tcttcgttgg ttctcaccca atccattgcg
    11221 gcataatccc ttataaggct tcccagagct gaataccttt cattgttggt tgcctcttta
    11281 aaagcctttg catgcatggt cataagcttt tttaaataat cttccttgaa tttattctta
    11341 tcgacagaca tatttgcacc tcataacata aaattcagat aaaaattaat tatttcatct
    11401 attatacaat atctttacaa cattaaccaa ggataataat tgcaatttaa taacaagata
    11461 tgaaaaaggc acagctttgt tgcactttga cagtcgcatg aataattgtt tataattaag
    11521 aaatgatacc gcatcatacc gataaaaaag acaggaggaa cagttcatga attttcgcca
    11581 tgttacactg acggtcaaag atttggatgc atccatacat ttttacactg aaattgtagg
    11641 attacccgtt gacagacgct atcagccggg actcgatacc gagatagcct tcctgggaac
    11701 cggagaaaca aaagtagagc ttatctgcca taggaaccaa cctgatgcaa ttgtcggcaa
    11761 tgctgtttct gtcggatttg aagtggagga catcctcgag aagtttgaaa gctttaaagg
    11821 taccgatatt tctgttgtca gtgatataat acagccgaac caacacgtgc gcttcttttt
    11881 tgttgctgac cctgacggct ttcgggtaca atttttagaa agcttataga caatacagcg
    11941 catccctgcc gatgcttcgg cggggatgtg cttaagtgtt attcatgtct tcagattacc
    12001 gcttttttct caattatctc aggattgtcc gcatatccta ttatccgctt accctttgaa
    12061 acccttacgc ttttatccaa tatggcattt ataacccttg actcctcttc tatcgaacaa
    12121 ttctgcataa taatactgtc ttttactact gctccttttt taatctttac gcttcggaac
    12181 agaatactgt tctccacctg gccgtctatt gtgcatccat ttgctacaag tgaatttatt
    12241 accctcgcat tctctttata ttttgtagga ggctcatcct ttacttttgt atatatgagg
    12301 ccattcttaa aaaacaactc cttggaaata tccatattca aaagctgcat actcgtcttg
    12361 taatagctgt aagctgaatt aatgcaggcc agatagcctt taaatccgta tccgaatatc
    12421 ttcagcttat gcaggtactt atacactgct tccctgaagt aatcgcagta gcctgttgaa
    12481 acacagctgt tcacaatatc aagaaacagt gattttttca ttatgtacat atccatggat
    12541 acattgcatt cattttcatt tccaaagttt atatcaatac gctcaaccct tccgtattca
    12601 tccatttcaa gtgtgttgca gtactggaaa tccacagtgc aattgtctgc tttcttatag
    12661 ataattgtta tatccgcccc ggtttcattg tgctgcttta ttgcttccca gtaatccagg
    12721 ttgcatatca tgttgctggg ggttattatc acattatttt gtttgctgaa atttaaatag
    12781 tccatatggc atttgaaatt ctcgatatcg cccctgtaaa tacccaagtt gtaataatca
    12841 tacattggat tcattatgaa cagtccgtca ttttttctgt ccagatccca tgctttccct
    12901 gtccccaagt gatccaaaag ggatctgtac ttgttctgcg taaatatgct tatattctgg
    12961 actccggcat ttaccaggtt tgaaagagcg aagtctatca tcctgtatct tcctgctatc
    13021 ggtaccgctg ctaaaggtct atggcctgtc aatcctttta tattgtcctc tctctcattc
    13081 aggttgatga ttcccatata atccagcata gtatcccctc cttcagttta ttctaccgaa
    13141 tccttagata tataccgttc cttggggcag ctgctcatat tctcctataa gtgttatgga
    13201 tcctgatgta ctgtcaccga tcctgcagcc gttattaata actgattcca aaccgattat
    13261 ggcacgttct actactacgt tttcaccaat cataaccttg ggcatcacca cagtgtctat
    13321 aacttttgtg tccttgccga tgtagacccc cgggaacagg acggaattcc tgacttcccc
    13381 gtgtatgaca cagccttcgc ctaccatgga gcactttatg tcagctgttg ctcccgcaaa
    13441 atgaggcggc tttattgcat taaccgagta tatcttccat ttggggtcaa aaagattaag
    13501 cctgttatcc tccttcagaa gatccatatt agcttcccac aagctttcaa tagtcccgac
    13561 atccttccag tacccgtcaa agggatatgc aaaaagcctt attccgctgt tcagcatctt
    13621 gggtatgatg tttttgccga aatcattgct ggattccgga tctgcttcat cctctacaag
    13681 gtattttttc aggatatccc agttgaatat atatataccc attgaagcaa ggttgctttt
    13741 tgaatttctt ggtttctcgg caaactcttc tatcctgccg tcaggcccgg tattcataat
    13801 cccgaatctg catgtctcct cccatgggac ttcaattact gcaatagttg cttctgccga
    13861 tgtttcctta tgatagtcca gcatctttga gtaatccatt ttgtagatgt ggtctcccga
    13921 tagtactaca acataatccg gatcatactc atcaacaaaa ttaatattct gatatattgc
    13981 atttgcagtg cctttatacc attctccccc cctttcattc acataaggag gcagtagtgt
    14041 gactccgcct ttctttctat ccaggtccca agggctccct atccctatat acgaattcaa
    14101 tacaaggggc tgatactgtg tcaatacacc tacagtatca attcctgagt tggaacaatt
    14161 actcaataca aaatcaatga ttctgtattt tcctccgaag tacacagcgg gctttgcaat
    14221 ctttttggta agcaatgtaa gcctgcttcc ctgcccgcca gccaggatca ttgctatcca
    14281 ttcctttttc ttcatgcgta cccctcctct aattaatatg tctatccata tttattaaag
    14341 cccccgatga tttattctaa aaaacaggtc tattattttg ccgctgcggt ttccagctgt
    14401 actgtttcca tttttctttc attctccgga atggctgcag gttttaaata caatactgcc
    14461 aaaggcggta cattaatttt gatcatctgc tgccggttgt gccattcctt cttaacagct
    14521 gaaaatatta aggtactatt gaagcctgaa cctccgaact cctgacggtc actgttgaat
    14581 atctcataat agctgcagtt gtacggcact ccgattttat agccctcgta tactgcggga
    14641 gtgaagttgc atactgcaac aacggtatct tccggccttt tccccttacg caggaaggtt
    14701 attatgcttt gcatcgaatt atccgcatca atccattcaa acccttcaaa tacatgatcc
    14761 agttcccaaa gggccggttc ccttgcatat acctgattaa gcatttttac atagttctgt
    14821 atccccctgt gcatttcata atccagcagc atccaatcca agggatggtc atacctccac
    14881 tctataaact gtcccagctc ccctcccatg aaaagcagct tcttccccgg atgcgctgcc
    14941 atatacccca gaaaaactct aaggtttgca aatttttgcc aatagtcccc gggcatttta
    15001 tcaagcaagg acttcttccc atggacaacc tcgtcatggg acagcggaag aacataattc
    15061 tctgaatatg catacataag agaaaaggtg agaaggttgt ggtgccgctt cctgtccgaa
    15121 ggatctgttt tcatgtattt aagcatatcg ttcatccagc ccatattcca tttatagttg
    15181 aagcccagtc cgcccagata ggtgggggcc gtcacaaggg gccaggcggt ggactcttcg
    15241 gctatcatca ttacaccggg aaaatactca aaaacaactt tattaagctt cctgacaaaa
    15301 tccacggcct caagattttc cctgcccccg aaaatattgg gtatccattc tcccggttcc
    15361 ctgtcataat caaggtacag catgcttgca actgcatcaa ctctcaggcc gtctacatga
    15421 tatatgtcaa accagaacac cgcatttgat attaaaaagc tatgcacttc gggcttccca
    15481 agatcaaaat tgcaggttcc ccatccgtaa ttttcgcctt ttttgggatc ttcgtactca
    15541 taaagggttg taccgtcaaa aagcctcagg ccatgatcat ccttgcagaa atgccccggt
    15601 acccagtcca gtatcacccc gatgccctcc tgatggcatt tgtctacaaa gtacatgaaa
    15661 tcataaggcg agccatacct gctgttgact gagtagtatc ccgtggactg atacccccag
    15721 gaaccgtcaa aaggatgctc tgtaaggggc atcatctcaa tatgagaaaa gcccatattc
    15781 tttacatagt ccaccagttc ttccgcgagt tccctatagg aataatacct accatcgggt
    15841 ttttgcctcc atgagcctgc atgtacctca taaatgttta cagggctttc ataaatattt
    15901 gttctttttc tattctccat ccaattgccg tcattccatt tatagcttcc aagggaaaca
    15961 agctttgatg cgctgcccgg ccgaagctct gaataaaacg caaaggggtc tgattttgca
    16021 ttaactcttc catccctgga gtgcatctca tatttgtaca tatcccactc tcttaagccg
    16081 ggaacaaata ctgaccatat tccggaatca tcgtccttta ccatgggatt ggcgccgctt
    16141 ctccaattgt taaaatcccc tatcagatgc acagcatcgg cattgggagc ccatacaagg
    16201 aattgcgctc cgtcagtacc gttaatatta gtgaaatgtg cacccaaaaa accataactc
    16261 ctatagtggt tcccctccct gaagagataa gcgtcatatg aactgtgggg ttgcccctcc
    16321 ataagcatct cctccctggc ttattcatca ggctatttgc cttttctctt ttttcccccg
    16381 aacatcttcc cggatttttc ggcgtatcga aaacacgcat agcacaatca taaatcctta
    16441 tatattatga tattataatt cgactcatat tatatatttc ctgctaatac taaatttaac
    16501 attacggcac aattgctctt ggaaaaattt gcttttcaca agtgcataac tgtagtataa
    16561 tgaaactaat tagacaggct gaatgctaac ccgtaagcct tttgagagga gatatccaat
    16621 tgaaaaaatc tgcttacaaa agaatagcat tagctatgct aaccctgatc atacttttta
    16681 ttacggttat tccgtcttat gccgacacat ccgtatacaa aagaggagat tcttcggcgg
    16741 aaattgcaga gattcaggct gcacttaaaa agctcggctt ttttgacgag gtctgcacag
    16801 gctacttcgg agaagttacc gaaggcgcag tattgaaatt tcaagaaaaa tataacttag
    16861 gcaccgatgg aataataggc cccataacca tcggaaagct taatgagctg acgggagggc
    16921 atattgcaat cccaagccgt tcctcctccg ccaaaaaaga cactacaaag gttaatgtcc
    16981 ggtataaaat aaaaaagggg gataccatat gggatattgc gggtgactat aatgtctccg
    17041 tagaatccat ccttgattat aacggactta atgaaaaaag catacttacc ataggtgatg
    17101 aaataataat tcccaatgtt gtaaaagtag tgaagcctgc ccctgtgcag acggtacctc
    17161 cgccctccgt gcaggacagt tcggagcttg gcaatatata tgagcataac aaaaatatat
    17221 atgtggtaaa ggaaggagat acaatagaat ccatagcgga ggattatggt gtttctgcag
    17281 agtcaataaa gaatgaaaac aattttaata atgataccgt tattcagatt ggacaggaaa
    17341 taaaaattcc tgaaggaacc gatcaagatg ttgaagaaga tactacggaa gcttccgatg
    17401 cctccggcac aaagggcgaa taccttgatt ggttcaaaga tgttcagtac atatacgata
    17461 agggtgacaa agccacagtc accgatgtag agacaggcaa atcctttaat gtccggagat
    17521 tgtacggcag aaaccacgcc gataccgaac ctctgacccc tgaggataca aaaatcatga
    17581 agagcatata cggcggaagc tggagttgga acagaaggcc tataatatta actatagaag
    17641 atgatgaggg cgttctgaga aaaattgcag cctccatgaa cggaatgccc cacgggggag
    17701 aagatataac cgataatgga atgaacgggc acttctgcat acacttcagg aacagcagga
    17761 ctcacagcgg aagcagatca gatccggggc accagaaagc agtcaagata gccgcaggat
    17821 tagaataggc agggccctga ggttacaaag ggcgggtaac acagaataca cggatgtctt
    17881 tgttttctcc accgtgtttt tagtggattc ccgttttttt gctactcatt gctttacccg
    17941 cagctcaaat caggcttccg gtgtttgaag aatgttttca taattttatt tacattttgc
    18001 aatttagtgt tataattaaa ttatataata tatttattaa tatttattat tgatagcttt
    18061 cgtactatat ttatcaaatt tgacaaattt ttcctaatta atattgacaa aaacaaatta
    18121 ttacattatg atttttgtat gccgcattgt gataaaattt atcataatga agatagctgt
    18181 aatgtatgca tcttgctgca gtttccggtt cattcaggta gtatagcgta tcaatatgga
    18241 ttattagctc ctttttgata aaaaataatt tcgctggagg aatgaatatg attgatttaa
    18301 ttaaattctt aaaggaaaag ggctataaag ttactcccca gcgtatagct gtttacgaca
    18361 tactgaagaa tacaaaagag catccaagtg ttgacatgct gtacagcaag cttcagccgg
    18421 tatatcccac aatgagcctg gcaacagtat ataaatccct tgaggtattc aaggatcttg
    18481 ggttggttca agagctcaat gtaggggaag ataaattccg atatgatgca aatgtaaatc
    18541 aacatcctca cattacttgc atagcttgtg gaaaagttga agacgtatac gatgaaatgc
    18601 tttttgacct tgcggatcaa gtgcagaaaa agacgggtta ccagcttacc aatcaacagc
    18661 tttatttctt cggctattgt cccagctgca aacgcaatca ctaaacgatt ttccggatac
    18721 gaggcacgaa acacggggac acggggggga cacggggacg tttcgcctgt cccgctttca
    18781 gcgggacagg cgaaacgtcc ccatgtctcc ccccatgtcc ccgtctccat gtcccattct
    18841 tttttcactg tactaacata gattcattaa gggcatttct tactgccttt attaccttgc
    18901 tcaacacata ggaggcagtt cccgtatcac ttgcagctgc cacatcctgt ttctttatta
    18961 ttgtatagat tacgtcggaa atttcatcct tgatgttggg attgttgctt atgtagtcgg
    19021 ggggcatttc gataagcttt atgtccttaa tttttgcgtt ttttattgtt acctccgcct
    19081 tgacatcccc ggattccgtt ttctctgcac ctacatatac cccatcattg tatttcaaat
    19141 atgtatagcc tgaagcagca agccccgctt cattgcttct taatgtaaac aataagacca
    19201 agccaataac cagaacaatg aataatacag cataaattat gagatttttc ttggttaata
    19261 aaataattct cggtttgata aatatccctc ccatcttagt caattacttt tagccagtta
    19321 tatttcttta tataaagtta ttacggaagg aataaatata tgcaaccttt taaaaaaaag
    19381 gtgcctcaag atatactttt ttcgctgtga taccggcaat cctacaaggg attgttattg
    19441 acagaattag tccacttgcc gcatctgtcc ccccacattg ccgcaacctt gccgtccatt
    19501 tcaaccttaa ttacttcaca ggaattgtca cagtcggagc actcaaagct tgcaggcaca
    19561 aaatcaaagt catcggcctc aaatcctctg aaccctgttg ttttgccgct ccttaaaata
    19621 tactccctgg caagcattgc cgcacctatt gcccccatta cgttgtaatg cccgggaaca
    19681 acaacctgat gccctattac cttttcaaag gctgttttta tacctttgtt tgcggctact
    19741 cctccctgaa atacaaaagg cggttcaagc tttttccctc ttccaagatt gtttatgtaa
    19801 ttcctcacaa gagcttcaca caatccattg ataatgtcgg cttttgagaa gccatactgc
    19861 tgcttggcaa tcatatcgga ttcagcaaac actgtacacc tgccggcgat cctgacgttc
    19921 ctttcagctc tcagagcaag gtctccgaac tcctcgattc tcaccttaag cctttcggcc
    19981 tgatgttcca ggaaggaacc tgtacctgca gcgcacactg tgttcattgc aaaatcaact
    20041 accattccat tcttaagcat tataatcttg gaatcctgtc cgccgatttc aaatatggtt
    20101 cttgcgtcag gaaaatatgt aatagcagca gttgcatgag cagttatttc attttttact
    20161 acatctgctc ccaccattat gccgctaagc tgtctgccgc tgccggtaac acctgcgcct
    20221 aaaacctttg agccgctgcc cagctctgct ttaaggtttc tcagtccctc cttcaccgat
    20281 tcaagaggct ggccgtttgt tctgatatat tgctttgata atacattgaa gctgtcatcc
    20341 actgctacta catttgtact cacagatcct acgtctatac ctatgtagaa cttcatcatt
    20401 tacctccgat tctgcttaag taaattctca acttcaagtg ttccctataa gttatttaag
    20461 taatcaaaaa aacatcataa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 gaatcaacaa tgaaaatgaa aattgcggcc ttgctccatg atataggaaa gcttgatgca
    20641 aatgcagtaa aggacttaaa ggagattatt tagcactgtc tttaatctcc tccattacat
    20701 ttcctattat ggatattcct tccgcgattt ttccgaggct tgcagcggca aagcttatcc
    20761 ttatatgcat ctctccggcc tcccccttta tgaatacggt gcccggcgta aaaatcactt
    20821 tcctgtctct gcacctttca aaaagttcaa cggatgatac cttctccggc agcttgaccc
    20881 acagattcaa ccctccataa ggctgggtga agcttgcccc cctaagatac tttcttgcag
    20941 atcttactgc ttcaaaatac ctcaggctgt attcccgctt catatattcc aagtgcttgc
    21001 tccatatgcc gcttctgaaa tacaaatcaa gaaccctttg cattaatcct gaggtatata
    21061 tgtctgttat atgcttgatg cccataacat tttcctttaa gccttccgga attatcagat
    21121 atcccactct aagccccggc atgaatatct tggaaaagct ttttatatat atcaccctgc
    21181 cttccctgtc aagcgcctta agaggccttt tccctacgtc aagataactc aggtcgctca
    21241 tgtaatcatc ttcaaccacc gtaacatcat attttttgca tagcagcaga agatatttcc
    21301 tcttcctttc agaataggtg tatccggtag gattctgaaa gttcggcata acatatataa
    21361 gcttgggctt aataagcgca agcttttttt ccagttcctt catatccggg ccgtctgcca
    21421 taagcggtat ttcaacaatc tttgcccccc ttgacttgaa tacggctaca gccccgttat
    21481 atgtcggact ctctacaaag acgcagtctc cgttggttaa aagaactctg gatataatat
    21541 ctataccctg ctgtgcaccg gaaataacgt gtatatcggt ggcttccgcg ttaattccat
    21601 attccgtcag gtattttgat accgactccc ttaagggttt ataccccata ttttcatgat
    21661 acatgaaggc tcttcccccg tcccgttcca atacctcatc cagcatcctc ctgaagtctt
    21721 ttatggggaa aagctttggg tccggggaag ctgttgaaag atttatgata tcctcatcag
    21781 ctcccatatc catgtcaaac tctatttcat ccaaagagta ggtgaaattg ctttcaaggt
    21841 ttatcacagc cttcagcggc attacgtagg ttccgcttcc caccttctta taaaccagct
    21901 tgttatcctc caacagcttg tatgcattga ctatggttat actgttcact cccagatgct
    21961 cagcgaagtc tctgattgtt ggaagcttgc agtcttccgt aacaatacct tcaattatat
    22021 attttttcaa ctgctcatac agctgtatat aaatcggact gccgctgctt ttgtctatat
    22081 ttatttctat atcttccgga attatcactt cacccatacc ctcacccctt tttgtatcga
    22141 tacaattatt gtagcatatt tatgtataca cataaagaat atgcggaatt tttccgataa
    22201 cttatataat atctatacca aagcaaaaaa ggattgcggc tttggcgtaa tgtcacacgg
    22261 aggtggaaaa atgaagatcg agaattcaaa catctgttta aagagcgagc actcctcggt
    22321 aacctttaaa cagaaacagg aaaacatgca gttctggata ggtgataatc ctgaaacatc
    22381 aatgaatagc cctcccggaa tcatcattga tatctcaaaa aaagccatgg aacttcatca
    22441 gcaaatcagc agcaccaatt cccaatgcat tgatactgat attgaagagg agcttgccgg
    22501 agaagacaaa caaaagctta tgcttatcga agcaatgctg acacagcttc tgggcaaaaa
    22561 attcagattc aaaataccga aaaagatcag aatcaacagt gatatgccca ttaaccgggg
    22621 tgttccggca aatcaaggca acgggaaccc aagagccgga tggggattga tctacgacag
    22681 caaatccatt cattatgaaa gtgaaaaggt gtccttcagc tcaaccggaa ctataagaac
    22741 ttccgacggg agggaaatta aaatagacct ccaactgaat atgagcaggg agtttttctc
    22801 aagtgaagaa atccacataa gagcggggga tgcgttaaga aaggatcccc ttgtcataaa
    22861 tttcgatgct ccttcggcaa ttctgtcaga caataaattc agtttcgata ttgactgtga
    22921 cggaagccct gaccagatat cccggctgct tgcgggaagc ggtttccttt ctctcgatct
    22981 caacaatgac gggaaaataa atgacggaag cgagcttttc ggccctcaaa gcggcgacgg
    23041 ctttgctgat ttatcacaat atgatttgga tgaaaatgga tggatagacg aaaacgacat
    23101 aattttcgac agacttagaa tatggacaaa ggacgaatat ggaaatgaca cgctttttgc
    23161 tctcggcgag aagggtatag gcgcaatata cctgggcagc gcaggaacgg ccttcagctt
    23221 gagaggagaa gaaaacaagg aaaacggagt cattcagaaa accggcattt tccttcatga
    23281 gaacggtact gccggatcgg tgcagcatgt ggatttgata gtttaaaagg taagaagcgc
    23341 acttcttacc tttctacttt ttctaccatc tgtatttttg taccgtcgct tttcatctta
    23401 aataatctgt atgaggcaga atcaacaatg aagtacagcc atccgtcagc gatatttata
    23461 aacttggagc tttcgtagtt cagaattgcc ttatctgtgc catccttttt tatcctgaat
    23521 atcctgttgc cgttatatcc attgctataa tatacccatt cctccgatac atttatgaag
    23581 agggcttctt cctttgtaag ctcagtattc tcagtaccgt cagtcctgat tttatagagc
    23641 ttgttccctt cagagccgtt gctgtagtat atccagtctc cgtccacatt tatatatgct
    23701 gccgaatcct ccgacactat gacattttcc gttccttcaa tattgacttt atatatccgc
    23761 tttttagcgt ctccgttagt gtaataaatc cacccatcat gcacattcaa atatgaggaa
    23821 aagttgtccc cgatgagtgt tttttccgta ccgtcagttt tgatcttgta aattcgcctg
    23881 ttatcggaag cattgctgta ataaatccat tcatctacaa catttatgaa ggatgcagag
    23941 tcgttagtca gctttgtcct tttagtgccg tctgttttta ctttatatat gctgcttttt
    24001 tccgtttctt caatattgct gtaataaacc cagtctccct gcacattcag gaacattgac
    24061 ttatcactgt ttaatttggt tttttccgat ccgtttattc tcattttgta tatatttaca
    24121 tcatcattgc cgttgcagta atatacccag ccttcctgaa atgccacaac accgccgttt
    24181 gttatatttc ccagggtatt gccgtaaccc tctgaaggca ttccgaccat atcttcaggg
    24241 agatccgccc ccttgataaa gcagcctgaa acagcaaaca taattaaaag caccaatgca
    24301 aaaattctga taagcttctt catttatttc ccctcacata tttattccct tcatatattc
    24361 tacacagttt gcgattttcc tttaactgta acgtaatata nnnnnnnnnn nnnnnnnnnn
    24421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn cattattctt cttatcaggc aagtgcaaag gtttatgctg
    24541 tactgctgcc gcaaaaaaag ctataattat ataaagcatc taaggctaat tctttacata
    24601 tcgggaataa taaagcaata cgaattaatt atgcgaggtg aacctatgag cgttcccagc
    24661 tttaaaaggc ttccgatgca cattggagtt attcccgacg gaaacaggag atgggccctg
    24721 ggaaagggct tagagaagca ggatggatac tgccacggaa taaaaccggg ctttgagctt
    24781 tacaaactat gtattgagct tggaataaaa gagttaaccc tatacggctt tacagaagac
    24841 aataccagga ggccctccat acagacacaa gctttcagaa aagcttgcgt cgatgctgta
    24901 atggaactta cagggaagga tgcggagcta ttggtcattg ggaataccag ctccccaatg
    24961 tttccccaag agcttcttcc ttataccaag agggcaaagt tcgggcgggg gcttataaaa
    25021 atcaacttcc tggtcaatta tggatggaac tgggatttga accatccgga gcatattgcc
    25081 tctgccgaca tttcaagagt ggacctgatt atccgatggg gcgggagacg caggctcagc
    25141 ggttttctgc ctatacaatc agtgtacgct gatttttata ttgtcgatga cctgtggccg
    25201 gattttaaac cggagcatct gtataaagcc cttgagtggt atcagtatca ggatgtgacc
    25261 cttggaggat aaaaacggag ggacggtata tgaaatacga tattgaaatc cccatacccc
    25321 ggaaggttca atttatactg gataagctgc atgagcacaa ttaccaagct ttcatcgtag
    25381 gaggctgcgt aagggacagc atacttggga aagtgcccga agactgggat attgcaactg
    25441 atgcgttacc cggagaggta aaagaattat tccaaaaaac cgcggatacg gggatcaggc
    25501 atggcaccgt cactgtaata attgagagaa gcctctttga ggttaccact taccgcatag
    25561 acggtgaata cgctgatttc aggaaaccca aggctgtaac ctttacttct tccattgaag
    25621 aagacctttc aagaagggat ttcaccataa atgccattgc ttacagccct gaagccggaa
    25681 ttgtggatcc ctttggcggt gtggcggata tcaggaaagg cattataagg acagtgggag
    25741 accctgaaag aaggttcagt gaagatgccc ttcgtatgct gagggctgta cgtttctccg
    25801 cacaactcgg attttctgtt gatgaaaaaa cgttgaaatc cattgaaata aacagcagtc
    25861 tgataggatg cataagctcc cagcgtataa gagatgagct tacaaaaata ctattatccg
    25921 ataaccccct ttgcttttcg ctgctgaagg atacaaagct gataaaatat actctgccgg
    25981 agtttgagcc ctgctttata accaatcaga acaatcccta ccattcgtat aatgtagctg
    26041 agcatacgct gcgctcagta gcccatgttg aaaaggacaa gatcctcaga tgggcaatgc
    26101 tgctgcatga tataggaaag ccgggtacaa aaaccacaga cagcaaagga atagatcatt
    26161 tttataatca ccagcagcta agtgtcaagc tttccggaac agccatgtca aggctgagat
    26221 ttgacaaaaa gtccatggac aaaatacttc tgctggtgcg atgccatgat atgcacataa
    26281 aggccgatgc aaaatcagtc aggaaagcca tgtccagagt tggagatgat atgtttgaaa
    26341 gccttcttaa ggtcattgag gcggataaaa aggcgcagaa ccccggactt cttaaggaac
    26401 gcaccgggaa attcataaaa atatgggaat tatataagga tatcaaggaa aaaggccagt
    26461 gtacaagcct gaaatctctt gcagtaaacg gtgatgacct tattgcattg ggtattaaac
    26521 ccgggaagaa aataaaagaa ctcctcaaca gccttttaga aaaggttatt gaagagcccg
    26581 agcttaacaa aaaggaaata ttactaagtt taattagcca acatcacaga gccgaatgat
    26641 attgccagag caaccagcca cattactgct actataatga tgctcttctt ttcaggaatg
    26701 cctgatactg cagatattcc gaataccata agtattgact gccagataac aaatggatca
    26761 aggttgttaa gcagtacgtc tttaaatgtt ggacttaaat ccagatatag aaggttgcct
    26821 gtgaaataca tgtaggcaaa ctttaccgct ataccaacaa tagatgccat atatgccaga
    26881 gtatagactg ccagaacctg tttgtatttt atttcaccct tgagtgctct gataaatgcc
    26941 ttatatacaa gtgcaaccag cagaatgatg gcgactgttg atattgctgc tacaacagca
    27001 gaaattaggt tcatcatagg cgtattcata aaagcaattg atgcccttaa tgcttcagcc
    27061 tgttcaggcg gcatagctgc tgccttttct tccatcattt caataaacaa atccttgcca
    27121 agaatcttgg ttccataggt atataagcct atcacaagag ctataacgaa cagcttcaat
    27181 gcccagacag gcttctcgat gtaatccctg aacagctccg ttggtttgat aaaaaacagc
    27241 tttagctttt taagcatttt attaccccca caaacaaaat attgagtccg ccatagatat
    27301 tatacagaat ttggaaccat aagtaaataa ggccttaggc cttattccaa ttcatgtatg
    27361 gtaacttttc ttgtgtgcag agtatggttc caatctttgt tcttcctcag caatgcttga
    27421 tgatatatta ttaattcata ttttgcagat agccaagagc cttttggctc aaataggtta
    27481 tcctgttctg aggcacagcg aactccttct ccacgaagga tcttcctcct tcattattgt
    27541 tgagccaaac catatagtat ataacaatat cgtccttcaa tacactggct ctgtcgtata
    27601 tcatgtcagg gcacctgccc aggcagtcag ataccgctga tattacatca ttcatcatgg
    27661 aaggaatcct gctcagggat attattattt catcttcggt ggcagtaacc actatcatac
    27721 tgtcgttttc catattcttc acagtcacta ccgactttct cattatggct tccgtcaatg
    27781 cgtccaataa tactctatga aattcaattt ccatatgaat acttatcctt tcctatacaa
    27841 ttaaaagtgc gtctcgcgcc gcactttttc gctagggaac cctatggttc ccttcgacgc
    27901 tgctctgcag ctgagcaccc tcctttatcg gcagggggct tccccccttg catcccccat
    27961 tgtataggaa agtatccttt cctatacaat taacattgcc gcagtaacaa ggtttgatac
    28021 caggtgggcg gttacggcac cataaaagcc ctttctctca tatatgaatg tacagtaaag
    28081 ccccaacccc aaaaaggtgg gaatgcctga tatattaaaa tgcagcagag taaaaattaa
    28141 tgttgatatg atggcagctg ccgcatgggg catacttttc agtaaaagct tatcataaat
    28201 ataatatctg aagatatatt cctctacaaa aggtgccaat attaccacca aaataaaaag
    28261 catcactttg tagtacatct ctccgccggc aaattcttct actatttctt gaggtttggc
    28321 gtccatattg aaatatatgt ttaaaatgta cacatataac ttatttatct gattgattac
    28381 cgctttaaaa accagggtgg caccggcaac aaaaataata tccccggctg ttgccgacca
    28441 tatattgata ttcctttcat gaatttcaac ctctgctttc ctgtgaactt tggaaagcag
    28501 aataagcacc ccgataaaag gagccagttc atgaatcaaa aacgcagcag accaataaac
    28561 cactcctacg gcaaactttg cgattaagct catatccttg aatatatatt cgtacagtga
    28621 gataaaaatc gggacattaa gcagcaaaaa agtaagaata ataataaatg cctcatatac
    28681 ctccaatagt gtccccccca atcaaataag cctacttcca ggtcagcagt acctcccaga
    28741 gttcctctct ccccacatgg gtctcagagg agtaaggaat taaaatatca ttttgattaa
    28801 tatcaagttt ttgcttaatt atattcaagt tcttgttcac tgatgaccgg gtgattttat
    28861 ccagcttcgt cgcaacaaca gttacagact ttctgtaata cttaataaaa tcatacataa
    28921 gtttatcatc agttgtgggc tcatggcgta tatctaccag cagaacaaca ttcttcaggt
    28981 tttccctgta cctcagataa ttctcaatta tcttacccca ggcctgcctc tggctttttg
    29041 acacggctgc atatccatag cccggtaaat caaccagaac aaactcatca ttaatcataa
    29101 aaaagttaat caatctggtc tttcctgggc tagagcttgt cttaacaagc ttcttacgat
    29161 taagaagcga attaataagc gaggattttc cgacattaga tctgccggaa aaagctattt
    29221 ccggcagatc aagctctgga tactggtttt ggtgaactgc acttttaata aactcacatt
    29281 tcttaattat catgtttctc atcccttatc aatgcatggg acaggacctc gtccatatct
    29341 gataccagca caaaggtcat gtttttcttt acggtatccg gaatttcctc aatgtccttt
    29401 tcattctcat aaggaagaat tactttcttt atgcctgcac gcttggcagc aagcaccttc
    29461 tccttcaaac cgcctatggg cagcaccctt ccccttaaag tgacttctcc ggtcattgct
    29521 acgtttctgt gcaccggaat ctttgcaaga gccgatacca atgctgttgt catggttatt
    29581 cctgctgaag ggccgtcctt tggtgtggca ccctccggga catgtatatg aatatccata
    29641 ttcttcgtaa attctttatc aatactaaat tcctcacatc tggagcgaat gtagcttaag
    29701 cccgctcttg cagactcctt cataacatct ccgagatgtc ccgtaagctc aagctttcct
    29761 gtgccgggca ttacggaaac ctcaatgaac aatgtatctc cgccgacagg tgtccaggcc
    29821 aaaccggtta ctacacctat ctcgttcttc tggtttatca aatcatgcct gtatttttga
    29881 atcccgagat attttttcag attaccggga gtaactctga ctcttttttt gttattctcc
    29941 actatttgag tagcgcattt tctaataata tttgcaattt cgcgctccag gttccttacc
    30001 cctgcttccc ttgtataacc gttaataacc gctcttatga cagcttcgga aatagaaacg
    30061 ctttcatcct tcagtgaatg ctcttttatc tgcttgggca ccagatagtc agcggctata
    30121 tgaagcttct cctcttccgt atatcctgaa atatatatta cctccatcct gtccaacaat
    30181 gccggaggta tggtatccgt cgaatttgcg gtggttaaga acataacctt tgaaagatcg
    30241 aagggcagtt ccagatagtg atccctaaat tccttgttct gttccgaatc aaggacttca
    30301 agcagtgcgg aagccggatc ccctctgaag tcactggcca gtttatcgat ttcatcgaag
    30361 aggaacaaag gattccttga acctgcctgc tttatggagt atataatacg gcccggaata
    30421 gctccgatat atgtcctgcg atgtcccctt atctccgctt catcacggac tccgcccagg
    30481 gacatcctgg caaacttcct ccccatagcc cttgcaatgg atctggctat ggaggttttg
    30541 cccactcccg gaggcccgac caggcacaat attggcccct tcatattgtt ggaaagctgc
    30601 ctgatagcca tgtactcaag cactctctcc ttgacctttt taagtccgta atgatcctcg
    30661 tcaagcacct ttcttgcatt ctttatatca aggttatctt ccgtcagagt attccaaggc
    30721 aaatcaaaaa tccaatccag gtaagtcctt gtcacgccta cctcgggaga gccctgggac
    30781 attttggaca gcctatccag ctccttttcc accttttcct ttacctcatc gggtacttcg
    30841 agctttgaaa gcttttcagc atattgctca atttcctctc cgtgtccttc cttttctccc
    30901 agttcccgct gaatagcctt aagctgctcc ctcagataat attccttctg ggacttgtct
    30961 atctgttttc tgactctgga gtttatcttc ctctctattt caagtatttc aagctccctg
    31021 gagagtattt caaatatttt ctccattctt tcctttacat caaacaagga caatatttcc
    31081 tgcttctctt ctatcttaat ggtgagattt gaggctatca cgtccgcaaa cctggacgca
    31141 tcatctatgt ctgataccgt gataagggtt tccggtgcaa ccttgggatt tgctttaata
    31201 tattcatcga aaacctccat tacacttctt cttaatgcct caagctccaa atctcccgac
    31261 agctgtacat cctgatgttc cagcacatcc acaaggaaaa aagcatcctc ggaaattata
    31321 tcccttatct ctgctctgtt gattccttca acaagcactc ttatggtgtc ccccggcagc
    31381 ttcagaagct gttttatctt tgatattgta ccaacttcat agaaatcttt cactgtgggc
    31441 aaatcagtat gagcatccct ctgagtaaca agaaatataa gctgatcatt tatcatagcc
    31501 tcttccagtg ctcttattga cttctccctg cctacatcaa agtgcaaaac catgtatgga
    31561 aaaatactta agcctctaag tggcagcaac ggcaatctct taatatcttc ttttcttttc
    31621 tgcattttat caactcctgt aataccatgt gtctaatcta ttatatcttt acataaattt
    31681 tttatcaata tttttaataa agatgtcctt ctttccatta ctgttttata tcgcttatag
    31741 cctgcattga aggagctttg gcaatttttg atgccgttag tatatttccc tcggaagact
    31801 gagccttcag gtgcttcact tccgcctgcc tcatggtggc tatgttgaca gcttcaacaa
    31861 tactttccac cgatattact tcaatatcat aagtactgaa tatctcctgc caattctcct
    31921 ttggaatgat gacctttttt acccctgcgc ggacagcagc ttcaatcttg ggaataatac
    31981 ctcctacgcc ctttactttc cctcttattg atacttctcc cgtcattgca atccggttgt
    32041 ctacgtagct gttggttatt gcggaatata tcgccactgc tattgtaata ccggcagaag
    32101 ggccgtccac cggtgtgcct ccgggaaaat tcaaatgaat gtcgtattcc cccggatcta
    32161 tgcccaaata ttttttgatt gcagtgatta cattgtcggt ggaagccttt gccatgcttt
    32221 ttcttttaat ggttcttccg tctcttgagc ctatttcctc ttcttcgact attcccgtga
    32281 ccagaagccg tccggttccc cggccggctc ttaccgcaat tgcttctatt tccataagag
    32341 tgcccatatt tggcccatat actgcaagtc cgtttacata gcctatttgc ggttcatcat
    32401 ttatcttcct ttccggcctt ggactgtact gtccgttgtt aagcacccat tctatatcgc
    32461 ttatagtaac tttattcctt ttctcattta tattaactcc gactgctgtt tgtataatgt
    32521 ttacagcttc ccttccgttt gtcgcatact tccctataag ctcgggaacc ccttcttcac
    32581 attcaaatct gatcttgtta agggcattgg aggtcactat cccgatttcc tcagaggtca
    32641 gtgccctgaa atatatctcg aggcatcttg accgcagtgc aggcggaatt gactccggac
    32701 cccttgtggt agccccgata agtctgaagt cagccggcag cccgttttga aagatatcat
    32761 gtatatggaa aggaatattg gcatcttccg agctgtaata tgcgctctcc aggaatacct
    32821 tcctgtcttc cagcactttg agcagcttat tcatttgtat aggatgcagt tctccaatct
    32881 cgtctatgaa gagtatccct ccatgggctt tggttactgc gccgactttt ggctgtggaa
    32941 ttcctgctac tcccatcgca cctgctccct gatatatggg gtcatgaacc gagcctataa
    33001 gaggatctgc aatacccctt tcatcaaatc ttaccgttgt ggcatcaacc tcaacaaact
    33061 ttgcatcctg gcggaagggg caattcggcg tctttttcgc ctcctccagt atcagtctgg
    33121 cagccgccgt cttccctatc ccgggagggc catatattat cacatgctgt ggatttgggc
    33181 tgcatattgc cgcccttaat gcctttatac cgttctcctg gcctattatc tccctgaaat
    33241 cagaaggccg ggtgatctca gacagcggct ttgtcagctg aatatctctc atatgctgca
    33301 gtttttccat ttcttttcgc gattcttttt ctatagcaac tttgtttccc tgttggcctc
    33361 tcagaaggtt taagaagtac attcctatta caacagcaaa aaaaagctgg atagcaataa
    33421 gcaattgtga catggcattt tcctccctca tatttgagtg tcaatagtat tattatattg
    33481 aaggcgggaa agtattcata accatgcatt cgaacagcag cagccaggag cggcaatccc
    33541 aaattaaaag accggttgct ctaaccggtc ttgtatttat gccgtacctt tccttgcttt
    33601 ctggcctttc ctgggctgct ttttcacagt cttctttgtc ccaggctcaa ggtatactat
    33661 atcgggctcc ttgtgctcca atatagtctc tcttttaact atgcacttat ctatatcatc
    33721 cctagacgga atctcaaaca ttacattgag catattctcc tcgaatatcg cccttaagcc
    33781 cctggctccc gttttcctgt ccaaagcttt ttgcgcaatc tcttcgatgg cgtcatcttc
    33841 aaattccaga tccacattgt caagctcaaa aagcttctga tactgtttaa ccagagcatt
    33901 cttcggctcg gttagtatct tcaccagtgc atctttatcc agaggattta acgtcactat
    33961 tatcggaatt cttccgacaa attccggtat gaggccgaat ttcagcaaat cctccggcat
    34021 tatcttttta agaatctctc cgatatcctg atgattcctg gaatgtatat ccgcaacaaa
    34081 gcccattgat tttttgcctg tcctgttcat tattatcttc tcaaggccgt caaaggctcc
    34141 gccgcatatg aacaatacat ttgtagtatc aatctgtatg aactcctgat gaggatgctt
    34201 ccttccgccc tggggaggaa cacttgccac agtgccttca agaatcttga gaagagcctg
    34261 ctgcacgcct tcaccggaaa catcccttgt tattgaagga ttctcagact ttcttgcaat
    34321 cttgtcaatc tcatcaatat acactatacc tttttgtgct ctttcaatgt cgtagtccgc
    34381 attctgaatc agcttcagaa gtatgttctc cacatcttct cccacataac cggcttcagt
    34441 cagggaagtt gcatccgcta tggcaaaagg tacattcagc atcttggcca gagtctgagc
    34501 aagcaatgtc tttcccgagc ctgtggggcc tataagtgct atattgctct tctgaagctc
    34561 tacatcatct atcttgtagt cggagtttat tcttttataa tggttgtata cagcaactgc
    34621 caatgctttt ttagcttctt cctggccaat aacatattgg tcaaggaact ccttgatctg
    34681 tgcaggcttt ggaacatcct gcagctctgt atcaaattcc tcatagaatt cctcgtcaat
    34741 gatttcctgg caaagctcaa tacattcgtc acaaatatac acacccggcc ctgctattaa
    34801 tcttctgacc tgctcctgat ttttcccgca gaaagaacat ttcagctgct ttttatcatc
    34861 aaatttagcc atgatatcac ctcactttac ttcctcttga caaggatatc atcaataagc
    34921 ccatattcct ttgcttctgc cgctgacatg aaataatccc tttcaacatc tctttctatt
    34981 ttttctatcg gctgtccggt ttttgcactg agaatctcat ttaatttctt gcggctcctg
    35041 ataagccttt ctgcatatat tcccacgtcg gtggccttgc cttgcgctcc gccggaaggc
    35101 tggtgaatca taacctcact gttcggaagg caaaatctct ttcctttcgc tccggctgcc
    35161 agcagaaatg cacccatact tgccgccaaa cctacgcata tggtagagac atcaggctta
    35221 atatactgca tggtatcata tatggcaaag cccgccgtta ttgatcctcc ggggctgttt
    35281 atataaaaat tgatatcttt atccggatcg tcagcttcca ggaataagag ctgcgctacc
    35341 accagacttg ctgtaacatc attgacttcg tcgcccagaa aaactatcct gtccttcaga
    35401 agccttgagt aaatatcgta agaacgttct cctctgttag tctgttctac aactatcggt
    35461 accaaactca taggtatcct ccctttccat gaaaataact atatggccac gctgttatcg
    35521 acaaggaagt caatggcctt ctggatgact atcccatcct ttatatatgt tatttcgtct
    35581 tctttcaaag ttttcttgaa tttttccaaa tcctgattgt aatgctttgc cgtcttctca
    35641 atttcggcat tcaaatcttc ttctgcagca gcaattccct caacttccgc cactttttcc
    35701 agcaccagct ggctcttcac tctgcttttt gctgtatccc tgtatgattc cttaagcttt
    35761 tcaatatcca tattcatata tttaaggtat gcttcaagat tcaagccctg atatctcagc
    35821 tgataatcaa agtctctcag cattattctt acttgtgcat ctaccattac ctcaggaata
    35881 tcaacagttg cattctcaac aaccttgttt atcacttcat cctcatactg ctgcttttcc
    35941 agtcttttgt actgctcttc cttctttttc cttatatctt ccttcagctc atccagagta
    36001 tcaaattcgc ttacatcctt ggcgaattcg tcatccaact cagctaactg tttttccttt
    36061 atttccttaa cggtaacctt gaataaggca ggttttcctg ccagctcttc gctattatat
    36121 tccttgggaa actgaacatt aacatctatt tccttgccta actccgcacc aaccagctgc
    36181 tcttcaaaac ccggtatgaa ctgtccggag cctacctcaa gactatagtt ctctgaagtg
    36241 ccgcccgcaa actgtttatc ctcaacaaag cccttaaagt caataattgt tatgtctccg
    36301 ttctttaccg gcctgtcgct tatatctatg agtctggcat tcttttctct tattgtctca
    36361 atttccttat ccacttcctc atcagttaca ttatactcct ttttttctgc ttctattcct
    36421 ttatactgtc ccaatgcaac ttcaggtttc acagtaacgg cagccttgaa aaccaagcct
    36481 ttaccgcttt caatatcaac tatgtctata tcagggcggt cgacaggctc cagattgtgt
    36541 tccttgacag cctcatcata tgcgtcagga catatgatat ttatggcatc ctcataaaat
    36601 accgcttcac cataatgcct ttctattatt ttccttggtg cttttccttt tctaaaaccg
    36661 gctatattga atttcttgac attcttcaag tatgacttca ttatcccttc ttcaagcttt
    36721 tcagccgata ctgatatctc caatgtcgca acattgtttt caagtttctc taattttgag
    36781 ttcatttgtt tcctccttaa ttatgtaata gtgtatcttt gtcataagct gtacaaaaca
    36841 ctataatcca ttcgcaaata ttagttgttg cttaatcccc tatattataa cataactgtt
    36901 accttaatca actttttttg aatttttctg ctattccact gccgattcaa gtatgcatac
    36961 ggttttgctg caggtatcaa ttgatacctc tgcgcctatg ggaactgtga agttaggctt
    37021 gcagtgtcct attttcaagc ccttcagaac aggcttcccc aagcccttga aaatgtcttc
    37081 aaaaacctct tcaaggctga ggcttttctc tggttcctcc ggctccgagt tcccccaatc
    37141 ccccagtata attcctgcgg cactcttaag cttgccggcc agcttgagct gtgtcagcat
    37201 tcggtctaca ctatagggtt cctcaaatac atcctccagc agcaatatcc tgtttcgggt
    37261 gtccacttcg taaggcgtac ccagagaagc cgcaactatt gaaagattgc cccccgccaa
    37321 tttacccgat gcttttccct cttctacaac ccccggggtt acaggattcg gcagctctcc
    37381 tgccggctcg gtgctttcca gaatgttttt catagctctc tttgtacgct tttccagccc
    37441 ccctgcaaaa tctcctccca tagggccgtg aaaagtaata agcccgcact tctggtaaat
    37501 tgcattcaat aaggctgtaa tatcactata gcccatgaat attttagggt tactgctaat
    37561 cctttcgaag tccaccaaat ccaatatgcg ctgtgaaccg taaccccccc ttaaacagaa
    37621 tacagcactt atctcatcat ccgcaaacat atcattgatg tcggaagccc tcaattcatc
    37681 cgcccccgcc aaataccccc tgctgctgta aaccgatttg cccgccttga ctgaatatcc
    37741 catatcggtt atatgtttga ttgccctttc tgcttttaag gtttccgatg ggcttgcagg
    37801 agccactatg cctattgtat ctccggtttt tagtctcttt gctttcataa taaacacctc
    37861 gttaacggtt tgatacagtt ttaccccaga attattctat cttgaagtca ataaattttg
    37921 tacccttata tgtcagcatt cccgtatcgc cttctgcata catttcggaa aaactgctgc
    37981 ttacaggaaa ctccagccgc tctccatttt cagtttcaaa ggcagcataa tagctgatac
    38041 ggatatccgt atccccatat ccgccccaat catgtgtcct cttcgctgca agccttgccg
    38101 ggatggtttt cttcggagcg cttttgtttc taagatattg tcccaatccc tccgtaaagg
    38161 atgttatgaa ggtcatggtc actatgacga aaataataaa cataaaaaac gggacaacag
    38221 aaaacaaaaa atcgttaaat gaatttgtaa atcccacaaa tattacctcc cgagtcttta
    38281 tcccaccaat atattgtaat caaaaagatg aaatatgtga atagaataaa tgaaggattc
    38341 cgtgcagagc atttaaatgt atgtacctat gtaaaataat accaggaaaa ctatctttca
    38401 tctatattat aacaaaaatg tatctgccga tgcacttaat ttgcaggaaa acaatagcgt
    38461 tccgcattac tgcggaacgc tactgaatct attgctactg cagtctttcc ggagttagcc
    38521 cccttacaag ctcgttaagc tcgtttctga agttatctcc taaattaccg tttccggcac
    38581 catcgctgat tcttctcaac ctttcaaaga aatttgtgtc agtgctgaca tatacagtct
    38641 gaatctgcgg attagctgct tttaccttct gggcacactg tcgttttacc gctgacagat
    38701 cgtttgcatt tccgtaggcg atatttctgc cggtaagatc accatcagta tctacgccta
    38761 tatagcatgt gttgcccgat atgacgcaag ttgcattatc gacaccgggc actgcttcac
    38821 aggctcttgc aagtctctgg gcttgggttg tattaagcct tgtattgttc attcctacgt
    38881 taggagaata attgctgcct cccatgttcc tgtagtcatt tccatatccc ccatacatac
    38941 cgttgttata gccgccatag ccgccataac ctccgtaata tgaagggaat tcaccatatc
    39001 cggtgtcata gggatttgtg gtgctgggtc caattcctgt atatgtgccg gtacggcctg
    39061 cagtatcctg ccccggctgt gccggtgtct gaagaggcct tcttgcagca caggcgctaa
    39121 acatcaatgc aaaacacaat accaaggccg atattacgat attttttctt ctcataggct
    39181 gttattcctc catatttttt attgtatagg aaagcgtact ttcctataca ataaaaaatc
    39241 tgagatagcc catcacaagc tgccttctct tttagttctg cgttaatatt ttttgcttaa
    39301 ataataataa tattaagaaa aaaagctgca attaatgcag cttttggatg tcaaccggtt
    39361 ttatttaaaa aaacagctat cgccaatgaa ttctctcagt attgaattgg aaggaattgc
    39421 attcatatct gcttccagga agtattgcaa aaatttctta attctctttg cttcaatata
    39481 ctcatgtact ccgttgtcaa tcttctcaag cagcggcagt acaatctgat ttagcatagc
    39541 cagctcatac accagggatg agttgaacat aacatccgcc tcctcctgga agggataaat
    39601 gtatttttcc tcacctcttc ttactgaatc ccaccgctta attgtggaaa gcgcatcggc
    39661 ggatctgaac tggtaatccc ttactatacg tcttatgagc cttaaatcgg atgtgggaat
    39721 cctgttatgg tcatctatgc tgagatgcgt aagggcacta atatatatct tgaacttctg
    39781 ctccttcggg atgcactgtg tgagcatttc attcagccca tgtattccct ccaccaccag
    39841 aatctgttct ttacctattt tcagttttct gcctttccat tccctgtttc ccgtatggaa
    39901 gttaaatacc ggcatttcca cttcttcact attaataagc ctcgctatga catcgttaaa
    39961 ggttgctata tcaattgcat ctatagtttc aaaatcatat tcgccatctt cacctatagg
    40021 agtatccgct ctgttcttga aaaagtcatc tatggatatt gctaccggct tcagtccgtt
    40081 taccctaagc tgaacagaca gccgctccgc aaaggttgtt ttacccgagg atgacgggcc
    40141 cgcaataagc acaagctttt tatttcctga agcaacaatc atatccgcaa tctgtgcaat
    40201 ctttttttca tgaagccctt ccgcaactcg tatcacgtca tttattctgc cgtccatgac
    40261 tgcatcattc agatggctga tattctccag ctccagaatc tttccccatt cttcaaactc
    40321 tctgaatatg ctgaagagtt tcttctgctc cttgaactca ggcagcttat tgggctctga
    40381 cctttccgga tatctaagga taagtcccgg tccattggct ttcagttcaa aaatcttgag
    40441 atatccggtg gaaggaacca tatagccata gaaataatca taatgtccgt gacagctgta
    40501 aatatttata tgctttttct ttcgatacct tatcaggttt accttatcaa actgctgagt
    40561 tcgtgtaaaa atttcgatag cctcggtact ggtcatcttc cttttaacaa agggtatatc
    40621 catattccgg aattcccgca ttttctcttc aattcttgca atatcttccc ctgccaatgg
    40681 cttatcctta tgaacctcgc aataaaggcc cttgctgagg gaatgctcta ccgtaaccct
    40741 gcagtcttta aagacctcct ttgccgcaat tataaaaaga aaagccaaac tcctggagta
    40801 aaacctgttc ccatacacag tagtcctgtc gatgaactgc aggctgcagt cctgccgcag
    40861 ctcaacccac aactccttta gttcattatt tactattgct ccgactatat ctgaattaaa
    40921 atgctgttta aaatcttttg caatctccag aagtttcata cccttgggat attcgcattt
    40981 agtattatcg gggaatgtca gagttatcat ttcttgcata accaaaacct ccaatcgtgt
    41041 tttacgcact aaacaaatta cattgaatct atatagatga ttatatcata aattcttcaa
    41101 aatttatgac atccgtccat gtgcatttta cgaggcaaat aatcacacgg gcttttagac
    41161 aaatatataa taattgcttt tattatacct catttttgct atattaacta aaatatagca
    41221 atttcgtatt caaattccaa aagttttata atacaatata tatatattgc ggatattgta
    41281 ttacagtgat taaagcacta aataccgtat tattaaaatg aaaggtgggt catgtatgct
    41341 taattacaaa atagtcggca atgccatgca gcaagtagtt gtcgaacttg gggcagacca
    41401 gacaatgtac agcgaggcag gaaaattcct gtggaaaacc gacaatatca agtttgagac
    41461 ttccttcaac gtaggtgaca aaaaagggca cttcgaagaa aaaagcggcg gaggcgttat
    41521 gggcaagttc atggataccg ctgttcaggt tggcaaaaga aagcttgcag gagaatccgc
    41581 agcttttcag gtatttaatg taacccatgg agagagcggg ttggtaacct tctcccagac
    41641 tattccggga gagataaaag caataaatat tgaggatttc ggcgtgctgt acgttcagaa
    41701 ggacggcttc ctggctgcag aaaatactgt agactttgat atcgcactta caaaaaaaat
    41761 aggagccgga ttcttcggcg gtgaaggctt catattggaa aaattcagtg atgtaggaac
    41821 ccttttcatt ggcgcctgcg gtaatttcat agaaatcaat cccgcggatt acggcggaaa
    41881 agtacaggta gataccggtg ctttggttgc ttttgataaa aatattgact atgacattga
    41941 atgggtaggc ggaagtgtgg ggcaggttgc aaagaacctt ctgttcggcg gtgaaggcct
    42001 tttccttgca actctttcag gaaacggaaa agtattaatc caaagcatga acatcacatc
    42061 ccttgccaga acacttttca gaaacgcaac aaaatcctcg cctgaggaca gaagcagcgg
    42121 caaaatgctt ggaggactcg gaagcctgct gggtgaatta ggcggagaca gatactgata
    42181 actagtgtac ataaaatgct ccaaagttgt aaacattttg taacaactgg cttcagacta
    42241 tatctttatg tatccttatt gaatataata ggaatataga tagtaataag tgctattact
    42301 ctttattcct ccccctcatt ttaaagaatg cgacatttat aggaaactat aaatgtcgca
    42361 tattgtttgc ccgtattcca catcatgtgc atttacattt ccctgattat tatatatatt
    42421 ataccttgaa agattgtttc gcagattata taaaaggaga atacagatga cagatactat
    42481 tctttttgat ttggacggaa ccttattgcc cctggatatg gagaagttca ctaaaatata
    42541 cttccatgag atgggaaaca tgttcaatga tatgatagac ccgaagctgc ttgccaaata
    42601 tatatgggct gctactttag atatggtcgg caatactgaa tacaaaacaa acgaagaggt
    42661 attcatggat agcttttcaa agcttattga cggtgatatc aataagtaca aggagcggtt
    42721 cgattatttc tatgataccc tgttccataa aacaaaagag gctgccgaaa tacaatcatt
    42781 aattattgag agtatttcaa tcctcaagaa aaaaaactac aagcttgtaa ttgcaaccaa
    42841 tccgttgttt ccccgcaaag cgatacaccg caggattgaa tgggcagggc ttgatttgaa
    42901 agattttgaa tacataactt catatgaaga caatcatttc tgcaaacccc agcctgaatt
    42961 ctacaaggag gtgctggata tcataggcaa gaggccggag cagtgcttga tggtcggaaa
    43021 tgatgttcag gaggatctta tagctcttaa gctcgggatc aaaacctttt tgataaagga
    43081 taatatgatt cataggacag aagaagcatt taccagcact tatgaaggca attacgagga
    43141 tttttacagc tttgtaaaag aacttcccga tgtcagccgc tcttcctgac tatggcattt
    43201 attgcccaaa taaaaggtat gagacaaatt gcagccacta taaaaaaatg ataattgccg
    43261 taataagctt taaataagga aataaaatta ttatagaaat gtgccagaaa acttatccag
    43321 atgttacctg tatataaggt ggctaagaac acaggtatgt aagatattcc aataagaagt
    43381 gcaattttta gcccccagcc gatagcatgc agcatcccga aagtcaaagc cgcaagaagt
    43441 atgctgataa taagcctgat attgccggga aacttcattt tattaaaagc caaagaaacc
    43501 agaagaagat taatcagttc ttctgtaaaa gctgctaaag gtaaggctat aatcttggca
    43561 aaaagctcaa tggggccgct tgtcactgca ctcatcttct gaagggcttc ggggaaaatt
    43621 cctgatgcaa taagcaaact gtttattgca ccaacaagaa acatcaaaaa aatcggatag
    43681 ataagtatta tgtccggttt gtatccgata aaacggggaa gtatattatt atccttagga
    43741 aagctcaggt atctttttat atccttgaaa agtataaggc acaagaccag cagagaagcg
    43801 tagaataaaa ttccgaaaaa aacagcatat aaacggcttg tcttcatatt aacaaaataa
    43861 agacacaaaa ccgtccaaaa gagcactatt gcgctctttg gcaggcttat gccatcataa
    43921 atattctttt ccaattcttt ataaattttt tccggtattt ttctcatgaa cacatgcctc
    43981 atattcataa taatacttga cctgattggc tttcacttat catattatat cataaatccg
    44041 caagtatcta ataaaaaaaa cactgcaaac cgttgcagtg tatacattct ttgatggtgc
    44101 gccatcaggg actcgaaccc gggacccatt gattaagagt caattgctct accaactgag
    44161 ctaatggcgc acgtttcgtc tattctgttc tcaatcgact ttataattat acaaaagaaa
    44221 attatctgtg tcaacatgtt attattggat taaattttaa tttttcagga tgatttatag
    44281 tctttcttcc gtgcggctca aatttccctt atatatttcc attaagctct cgtagctgtt
    44341 gccggagtat ttggaaacca acagccttat ttctgaaaga tgctttattg cattttcgaa
    44401 ttcaagcttt gcctttttaa aggaaccctc attataatat ttcactccgg ctaaattgca
    44461 ttcattggcc agcttgaagc atgcatctat tttttcagcc ttttcaagca ttctgtcctt
    44521 atttcccgca aaaagctctt taagtttgga ataaagctcc atatcatagt tgcttgtatt
    44581 tgatttcata gtttcaagca tgttcagcgc attgtcaaat tcctttactg cctgttcaag
    44641 cctgccttcg ttatattgct tcactccggc cagattgtgc tccctggcaa gctgcagaaa
    44701 agaatcagag tattgcctga aacctctgta agaggcatca acttttatta gttccgaaaa
    44761 cacttcaatt gcttttccgt attcattttt ttcatagtat tccaatgctt ttcccttcag
    44821 cttttcagct tcggttcttt tttttgctgt cttgatccgg ctttccgttt taactggttc
    44881 tgcagcaggt acagtagtca ataccgctgt tgccgccggt gccggaatta cctcggctgt
    44941 ctcggcaatt tcctcgcaag ccggctctaa tgttgccggt aaggcatcat aattcattat
    45001 gtatcctatc ataaatgcca gtaccattgc agcgacaatt atggctgcag cagaaaaaaa
    45061 ccttctcttt gaccctcctg atgcattatt gatggatgcg gcaatctccg ccactgctgt
    45121 tctttctgct tcctcctcgt ttttttttga aatctcaata ctctctttat cctccgcttg
    45181 aatttcttga ttgttcatcg ttgttttagc ctccgcctta agtatgcgtt caagcttttg
    45241 cctgaattcc gccggcgact tatatctgtc cttccagtca tagctgcagg ctttaaggat
    45301 aacagcccca agattttctt ccgaatctgc aggcagcggc agcctttccc ctgacatcct
    45361 gcgggctgca gacttttccg tatcttcaac ggtaatagct tccggaggca gcggcaaaaa
    45421 tggcagccgc cccttgttaa gcagcttgta cataactatt cccaaagaat acaggtcaac
    45481 ggaaaaatca tacgactgct ctttataaag ttcgggggcc atatataagg gattgagtgc
    45541 agtcaaaaca gtcctcccgc cctttgaaag ctcttttgcc atattgaagc tgcccaactt
    45601 gaactcttcc ctgggtgata caaatatatt tgaatcctta atgtccctgt gtattatgtt
    45661 ctcccttccg caggcttcaa gagccttgca gatatcaatc cccagcctga caacatcaag
    45721 ccttccgagt tttcttccaa ttagaaaatc gggcaatgcc tgaagagatt ccatccttat
    45781 aagaacatcc cacccctgat ttcctttctt ctcatatatc ccatggtctt cgtaagttac
    45841 aatattgctg ttccctctca gcttgtacat cagctcaatt tcgttctgta cgttgaccaa
    45901 caggctttta aagtattcag gcatagctgc cctgctgatg cctatggcct gagcctcgga
    45961 aatatcattt ttgcttatgg aaaagctcat gaatttgacg gttgaaatat accgtttacc
    46021 ccattcctcc ttaaacactc tgtatatttc acagtcaacc ccttgtccaa taagctcatc
    46081 taccttccat gacccccaaa gaggctggta tttctcaatg gccctgccgg attccctcat
    46141 ccaattcacc ccaaattcat ctgaagactc acttaattat gaaacctgca cctttaactc
    46201 ataacctata atctagtaat ttctatattc ggcagcggta tccttcatta tttcaatcgc
    46261 aagttcggac accatttttc tacatattca tacaaaggtt ctatggaaaa ttcatctgga
    46321 tttatccatg ctatataata tataatataa tatataatat aaagtaatat atttgggagg
    46381 gtgataatat gagtctgtta agccaaacaa ttcaatcagg tgactggaaa ggtgaaaagc
    46441 atgttcctgt aattcactgc cctgaaaagg taacgccgga tacccccttt gaagtaaagg
    46501 tgacaatcgg tgatgcaata ccccatccca atacctttga gcaccatata gtatggataa
    46561 aggtgttttt ccagccggaa ggctcaaaat ttcctgcaga aatagctcat ttcaacttct
    46621 cggctcatgg agaaggggag acctttaccg acccggcagg gctgacccgg ataaaaataa
    46681 aaaaatccgg aatcatttat gcattgtctt actgcaatat ccacgggttg tgggaaagca
    46741 gcaaagaaat cagtctttag gttaaaagaa agccctgatg tattatacat cagggcagtt
    46801 tcatgaattc gccttgctct ttaaaatgcc taatagcttt ctccgttggg aaaaacaaca
    46861 aataccttgc ggaaccatcc gttatggaag tctatttcaa aaaaatctac attgtcagat
    46921 atgtcattct gcacatagaa ggttctatct tctgcacgga ctatgatgtc atccggtttt
    46981 ggttcatcct gaaccaggct gtaagccata gacagtcatc cgcaatcatt atcatattct
    47041 attctgaagt tcttggattc aatttttgac agttcccctt tagctttatc agtaatcctg
    47101 acatccataa tatccctcca ttcgtgtttg aatgttagtt caattatgaa ttatttgata
    47161 aataaaatca agattaattt gtaatagtta ttaatttgtc aatatacagg atttcctgca
    47221 aaaagcggtt ataccgcagc tttcggctgc ggtacaaccc tggcattatt gaatctcaat
    47281 tctcttattt ttgcttactc ctgattccct ttttggaagt accattgata atacgccatt
    47341 ttcaaacttg gcagaaattt ggttttcaat tacattttca acatagaagc ttctgctgta
    47401 gcttccgctt cttctttctt ttctgatata gttttctttt tctatgtttg tttcttcgtt
    47461 tcttgttact gatattgtga gtctgtcatt attaaggtct acgttgattt cttccttttt
    47521 tactccgggt aaatctgctt caacaatata ctctttttca ttttccttta tatccacctt
    47581 aatttgtgca tcacctgcga agaatgcggg catgaaggaa tctccgaaga aaccgtcaaa
    47641 tagattgtct atgttgaaaa tatcatgagg cctctctgtt aagcctctat tttttctgcc
    47701 gtatggtacc atgaacatac aaaacacctc cgtataaatg tggtaatttt ggtcttaatt
    47761 tgactttgac tttctttgac ctttgtttat attatcacat ttattatggg aaatttcaat
    47821 tttcattatt ccttattttt tgttaaatta gttcaaattt acataatata taaaaatatc
    47881 ctcgtttttc tcaaaaaatc tttgttttaa atatttaccc ttctttattt ttcaataatt
    47941 catattttat tgtcgcagct gtacttgcat aattatcaat tattttaaat aatatgatat
    48001 aattattgca ataccgaagt acaaatactg catacggagg tgctgcaaat gattgaatat
    48061 ataaagaatt ggctggctct taaaggtatg aatgaaggaa tttctcttat tttatcaaat
    48121 attatcacag ctatcgctat tgtgcttctc agcatagtga taaatttgat tgtaaaaaaa
    48181 ttctttttga aaatcgttaa ggcatatgct gcaaaatccc ctcaaaagtg ggatgacatt
    48241 ttgctgaaga acaaattctt tgatcagata gtgaatattg tacccggatt tataatctac
    48301 gggtttgcac cggttatccc catgtatcag aacttgatcc agcggattgc ctccgcctat
    48361 atagttattg ctgttttatt cgttgtacat aagcttctga atgccgtgga tgatatttac
    48421 agaagcagtc aggaagaaag aacaaggccc ataaaagggt tccttcaggt tattgagatc
    48481 atttcaatta ttataggaat catattaatc atcagctttc ttataaatcg ttccccctgg
    48541 attatgctga gcggcatcgg agctgcaact gcaatatttt cacttatttt taaggattcc
    48601 atacttggat ttgtagccgg aattcagctt accgcaaata atatggtgaa gctgggtgat
    48661 tggattgaaa tgccgaaata tgatgcagac ggagatgtaa tagaaataac ccttcatact
    48721 gttaaggttc aaaattggaa taaaaccatc tctatgattc ccacttatgc attgatatca
    48781 gactcattca agaattggac cggaatgcag gaatttggcg gcaggagaat aaaaagacat
    48841 attaacatag acttaaccag tataaaattc tgcacagagg aaatgttgga aagattggta
    48901 aaaacccctt acctccagga atttctcagc agttacaata atgagcacat aaatgggaaa
    48961 cagctgacta atattgatgc tttcagagcc tatctggtgg gatatttgaa gaatcatccc
    49021 gggctcaaca aagaaatgac acaaatggta aggcagcgtc agccgactga atttggtctg
    49081 cctatagaaa tatatgtttt tttcgacgga acaaaatggg ctgattacga agtcgtgcaa
    49141 tcggaaatat tcagtcatgt cattgcaatg gttcaggaat ttgatttacg gatatatcag
    49201 aacccttcgg gatatgattt tagaaatctt ggcaaatgtg aatcttaata tgttcggttc
    49261 ggcttcctgc atctgcatat catatgtaga agttgtgtat gtttcctaat tacttcctct
    49321 tggaatcaga atccacctta agcctttcct gtgagaggga tacatagatt gcaaggggta
    49381 ccaggataca gctcaatagt attcttgtcg cggtatcctg aaatgtctgt attattgtaa
    49441 aaattacagc tattattggc agcagctgaa cattactgtt tttaaaaaac aaatatttca
    49501 tatccctttc tgccgatacg atactgttca aggaatatat ccccagttca accctgtatt
    49561 ttgacggaat accgatcagc gtacttgcat ctgcatcatc ataaacaaga acattggaat
    49621 tcttaagcac ctcaaaggaa tcaaagggca gcgcccttaa tattgcctca tgccctctgt
    49681 gcctcacaat cagcttatcc gtctcaccaa tgccgaggat tttcatggtt gtaggcatta
    49741 tacgcacaat atcgctgttt tcatctgtag gataaggcct tcctactctt aaacatattg
    49801 attttctttt aatgagaaaa gagagaattc catatataaa tcgtctccat cttcccccca
    49861 aacccatcgc ttttgtttta tcgccagtgt catttacttc aaccagctca attttatcaa
    49921 gctccagctt tttaaagatg ctttttaaat catcgtcttc aatcagattt tgttcaatct
    49981 cataatatga gtgccgatcc ttgtagctcc gttttaaaat atccttcgct gcttcggtga
    50041 gaacatcgct tcccaggtat ttcagaaaat catttcttaa gattactttc ttgggaatat
    50101 ttacattcat aatatgcctt tgatagtaat taagaaaaat cttgcctttt gcggtcgtat
    50161 cccttgtcac agacagcttc ccctggcagt agtcaatgtc atcatgcctg ttatacaatt
    50221 gatagttttt acttaagtca atcttatccg ccagatccgg gcatatcatg attctttccg
    50281 atccggcctt ggggattcca atcggcaggg ttattgcctt cttcctgttt accagcacat
    50341 attttctctc ttctgcctca ttatctccaa aaacgttttt gtataatttg ttggtcaggt
    50401 ataccttccc gggcacaaat tcctgattgt tgcccgtatg cttgtatctg gcaatgaatt
    50461 cggttttgct gcagtgatta aacacagaat tcacctgaaa ggaatcattt ctttgaaatc
    50521 cgtatttctc tgcatcttcc ctgctgaatt ctattttatc tataggaata tggattttgc
    50581 ttttttcagc ctcaaatacg tatgtgtcgg aatcccattc acagtcattc aatacggata
    50641 tgacggaata caggcctttc atgaaactca taaactcttc ctcgtttacg gatacttccc
    50701 tgtatttcct gttgacctcc acctggatag acggtatccc gcaaaccgcc gccattgttc
    50761 ttgatacgtt gttttgcggg cttgctgtga acatcttatt ttcttcaacc ttatgaattc
    50821 cgtatttgcc gaatactaca cggaacaggt cagggataaa gttatttccg caaaggttat
    50881 ttgaaccatc ggtccccagg tcgatgtcaa attccctgtt ctccgcagct ccatgcaggt
    50941 ctatgaatat acgtatattc tctttcctga taacgtctat tacgctgttc ttataatccc
    51001 cgtccccgtt tatgggatca tagttggggt catagttgtc gtttttggtt ttgtaaaaca
    51061 cgtggcaccc ggtcagcaag tgtatcattc tggcaattga accggtaaat atgtctgcac
    51121 attttacctt gccgttcctc aaatgattta ccgaatgggg tgccgatacc aaaaccggta
    51181 tggtaccctt ttcatattta taaccataat caatttttga atccccgtta tagttatcca
    51241 tggagaatgc ccgttcaaac aagagggtct ttttaactat gtattcgcgg cttttattgt
    51301 taatcatacg tacctcacaa atcaatttac ttctaaagat tcagcgtatc ctgcttaaaa
    51361 tactccttta catctctgac cagggactgt gttcctatta tgcacaccag cccgtcatcc
    51421 ccaatcagat tatatgccgt ttctattgca tcttcaatct cagcttcata tatcagcttt
    51481 tctcccagca taggctttgc tttgtccatt tgagctgttg taaatttcaa atgcttgttc
    51541 tttgaagcgg tgaaaataat ccttcttgat atgtcataca tttcacatgc aacgccttca
    51601 taatccttat catcgggaat cccgacaatg gagattacgt ctttatttcc catttcactt
    51661 acaaactccc tgacatgtat cgcgcactcc ctgttgatac acccgtcaag tatggttaaa
    51721 gggcttctgt ttatcacttc caaccttccc ggccatgtta tgccgtcaaa tgcctgtctt
    51781 atattctctt ctttgactcc ccccagcaaa ttctctgctg tgcaaaccgc aattgcggca
    51841 ttatatgcct gatggcgtcc catcaggttc agcctgatat ttctgtattt gttgtttcgg
    51901 gtaatgatat caaaataagt gccgtttctg tcaacgcata ttttttcaca caaaaaatcc
    51961 tgtccgaagg taaataatct gactccctta tcacaggctt ctctacagat aacatcccgg
    52021 acttccttct gctgtacggc actaactgca aacctctgga tgctgttgat tattcctgcc
    52081 ttattaaagg ctatctccgg aagattatat cccagctgcg gaatgtgttc cggaaaaata
    52141 gtgttgatga ccgaagcttc gctatatatc cttgttacat cgtcatatct tgctcctttt
    52201 ccgcattcga acacgttgta atcagtcttt tgatccagat aatacagcat ggcaataacg
    52261 gcagtaatac cggcaggccc tatatattgg gcctctgtca attgtttttg cacctccgct
    52321 gcataaggct ccagaatatt ggcatactta acaaaatccc cctctgaaat tgcattgccg
    52381 tttattctga ttcgctccaa aaagcttacc agatgcgggc ttgtacacaa tccggcctta
    52441 agcccgtgag cctctatcag ttttgatatc attctgctga cggagccttt ccccttgctc
    52501 cctgtcacca gtatattcct ttgttttttg tccggttctt ccgttttatc cagaagcgct
    52561 cttgtcagat gcgggtttct ggtcaccata tccggcatat cctttggtat cttatctatc
    52621 gcgttcatgt aggaactata tacgaaatcc tccgcttctt tagcatttgt aaaaatagca
    52681 accactccta tcatatgttg tgcaatttaa gaagaatgcg acggggacac ggggacgttt
    52741 cggggacatg gggacgtttc gcgtgtccca ctggaagtgg gacaggggaa acgtccccgt
    52801 gtccccgccc ccatgtcccc gcccccatgt ccccgtcgca ttattcttct tatgcaactc
    52861 attcaattat tattaattat tacattatgg ttttattttt aatctcctca acatatcaat
    52921 taaataacaa tactttgtcc tttaatataa cgtagggggg ataaaaagtg ttcaacaaaa
    52981 gaattctatt cttttgcacc gttcttgccc tgctggcagc agcacttcag gctgtgcccc
    53041 tcattttcgg agcagcattt gcttttggag cattattgaa tggatttcct atatatttag
    53101 cggcacggct cagctatata ccgggaatac ttgtttatct ttcagccgca gccctattgg
    53161 cttttttaaa tcccttcgaa gccctttttt tcatctgtac caacggcata atagggcttt
    53221 ccctgggcat tattaaagac cgcttcagga gtatctatcc tgtccctgca ctttctgcac
    53281 tgttggatgt cgcaatactg ttcacagtca attacaggtt cggcataagc attttcagcc
    53341 cctctgcctt tagcacccct gccggtcaag ctgccgccct tctgtttccc atgtatttgt
    53401 attgcttgat atacctgaag ctggcaatgt ctttggataa gcttattcac aggtacatag
    53461 aactggatat ccatccatga cttttatcgg ataaaagcca gaggatcact ttccgtaagc
    53521 gactcaggat ttatatacag agttcctatt gtagtggacc aatgcagatg cgggcctgtt
    53581 gaaaagcctg ttgatcccac ctctcctatg atgtcgcctt ttttaacttc agccccttcc
    53641 tccacaagaa gcttgtcaag atgggcatag gagctgtaga tattgcagcc atggtcaatt
    53701 attattgtat tcccagtaac gttaagcatc atggatagcc ttaccactcc attattggct
    53761 gccttcacag gagtccccct ggctgctgct atgtcgatcc ccgagtgcct tccggactct
    53821 tccttgttaa tgtatcttat cataccaaat tcggtagaaa cccttccttc caccggtttt
    53881 acaaaggtgc cctcccatag gggtttttcc gaagttacgg attttgctct ttcggtatga
    53941 accttatcca tatcaaaatt atcatcactt ctctgttcct tctgagtgga tgtaacagta
    54001 agatgctgct ccacaaactc tttatggaat accttaaagg tttcctcttt ttcagctgca
    54061 atttccccat ccctcagaac ctgaaccttg cagctgtaat ccccttcttt tgtcctgcag
    54121 cttgttccga ttatagccaa ccttccctcc ataaagctga aaaaccttgg ctgcccctgc
    54181 acaaaagcag tggatacctg aatatcatcc ctttccgtca gacccttgag atatattgcg
    54241 aagcagtctc cgggcagcag ttccgtctct gagaatacga tttccggcac aggctccggt
    54301 acaggctccg gttccggctc cgttaccggc tccgccttct cgggcacaaa aaaatcctcc
    54361 tgcaagccta tgacaccgca aacagaaaaa aacagaatac aaaatataag cgcttttata
    54421 aaatatctac ccatcccaaa ctactccttg cagcctgtaa agatacaggc agccatacct
    54481 tttgaacggc tgcctgtttc tcatattaca tttttacaaa accccttctg catacaaccc
    54541 atcaaaatta agcaatatat acatgctccc gtcatctttc tttcctacac cgtttatata
    54601 cttgctgccg atatttgata cgacttccga cacgggttct attttctctc tttccaccgt
    54661 aattacgttt gatgcttcat cgacaacgaa accggctata gtgcctccgg tatcggccac
    54721 gattatcttt gtattttctt ccgtgctctt atcttcaagc tgaaacctct tcttgaggct
    54781 tattacagga atgacattgc ccctcagatt aagcacccct tcaacataat cgggggtatt
    54841 gggtatcatt gtgggcttcc tgtaaggaac aatttcgtta accttaaata tgtctatggc
    54901 gtattcctgc tcattcaacc tgaataaaac caactgcatc atatccatta aagcaccccc
    54961 tttttaaatc aaccggatgt tcttataact tatatttcga cgtttttacg ggaattcctt
    55021 cttatctgca taataggatg ggggacatgg ggacgtttct cccggggaca cggggacgtt
    55081 tctcctgtcc cgctttcagc gggacaggag aaacgtcccc gtgtccccgg gagaaacgtc
    55141 cccgtgtcct acttctgctt ctgtgacgat aatttttttc acgatatata tatgttataa
    55201 ttaataaaat atatttgatt ttcaaccggc ggaggtaaag caatgaataa aataatgtac
    55261 aagagtacca gaggaagagg taggctgatt actgcatcgg ccgccatact taaaggcatc
    55321 gcagaagatg gaggcctttt tgtcccgact gaaataccaa ggcccgaaat ttccctggaa
    55381 aagatgataa atatggacta caaaagcctg gcataccaca ttatgaagga ttactttcag
    55441 gattttgaag aagccgaaat acgcgaatgt attgataaag cctatgatga aaaatttgat
    55501 accccggaaa ttgcacctgt agtaaaaaaa tcaggcgtgt attttctgga gctttttcac
    55561 gggccaaccc ttgccttcaa ggacatggca ttgtccatac tcccgtattt tctaaaaacc
    55621 gctgccaaaa agcagaagct ggatagcgaa atcgtaatac ttactgccac ctcgggagat
    55681 accgggaaag ccgccctgga aggctttgcc ggagtgcccg gcactaaaat tatcgtgttt
    55741 ttcccggaaa gcggagtcag tgaagttcag aaattgcaga tggttaccca gacaggcgaa
    55801 aatacatatg taataggcat acgaggaaat tttgacgatg ctcagaccgg tgtgaaggaa
    55861 atgttctccg acaaagagct gcaaatggat atgtcagccg caaacttcgt attttcttcc
    55921 gccaactcca taaatatagg caggctggtt ccccagatag tgtattattt ttatgcttat
    55981 ctgcagcttt gcagaatggg tgacattaaa gccggtgaag aaatcaactt taccgtaccc
    56041 accggaaact tcggcaatat actggcagga tatcatgcga aaaaaatggg gctccctgtc
    56101 aggcacctta tatgtgcttc aaacgaaaac aaggtgctgt atgatttcct gaacacggga
    56161 gtatatgata aaaagcgaaa tttcatcaca accatgtccc cctcaatgga tatacttgtt
    56221 tccagcaatc tggaaaggct tctgttttat ctttacggtg aagacagcag tatggtgggc
    56281 gaattaatgg cacaattgtc aggaaaaggt gcatacatga taaatgacaa tgtgaaagat
    56341 aaactaaatg atttttacgg atattatgca tctgaagcag acactgcgga agcaataaga
    56401 aaaatgtatg aagactgcgg atatgttatt gacactcata cagccgttgc tctttcagca
    56461 tacggaaaac acaggaagcg gaaatgcgat gaaaatataa aaactgtgat tgtttcaaca
    56521 gccagcccct ataaatttac gtcagacgta atgaaatcaa tagatcccaa gtactctggc
    56581 caaaacagct tccagctgat aaaagagatg agcagactta ccggggcaga aattccaaag
    56641 ggtatcaggg atctggacag caggcctgta attcataata ctgtttgcaa taaagatgaa
    56701 atgaagaaac aggcagaaaa aattctcggc ttatgattat tcaccgccga acttatagcc
    56761 tacaccataa acagtcaata tgtattttgg tgattttgca tccggttcaa tcttttggcg
    56821 gagattcttc acatgggtat ctatggttct gtcgtagcct tcataatctt cgcccagcac
    56881 aaggaaaaca agctcatcac gggtaaaagc cttttgaggg tatttcacca gggccattaa
    56941 aatcttgtat tcatttggag taaggttaac aggctttccg cctcttttta cctcatgtct
    57001 gatactgtct atcagcagct ccccatcatt gtaggacagc acatcggaca ggggtattat
    57061 atcatccccg gagcgtctta aaagagccat gacccttgct gtcaattgac gcaggctgaa
    57121 gggttttgta acataatcat cagctcctat tccaaggccg gtcagtatgc tttcctcttc
    57181 cacccttgca gtaagcatta ttatcggtac tcttgatttt tttcttatac tcctgcaaat
    57241 atcctcgccg gacatgtcag gaagcatcaa atccaatatt atcagatcgg gatttatcct
    57301 ctcaaaaagc tccagagcat tgctcccgtt ataggctgta taaactttat aaccgctttt
    57361 cgtcaaatat gattcaacta cttctgcaat cttctcttca tcgtcaacca caagtatcct
    57421 ttttgcattt tcgttcacgg caatccctcc cttatgggca ttataacata acatactacc
    57481 ctaatattcc caagtgctca aaaagaatct tcagcccgat acatatcagt atgaatcctc
    57541 cgactttctc agctttcttt ttcatcaagc agccgcactt gtgtcctata tatactcctg
    57601 caaagcatat tgcaaaggtt attgctccaa tcaaaaccgc agattggact attgaaactc
    57661 tcaaaaatgc aaagcttata ccgaccgcca gagcatctat gcttgtagcc acggcaagaa
    57721 caatcagtct gcccatgctc agcggattct cacatgctgt atcctgattt gatacggcca
    57781 cctcgcacat tccctcaacg ccttcttctt tatcctcccc gcaagcctcc cgtatcatct
    57841 ttcctcctat gaaggccaac aaaccaaagg ctatccaatg gtcgatactt actatgtatt
    57901 ctttgaactg ggaacctgca atccagccta ttaaaggcat caaggcctga aacaccccga
    57961 aatacaagcc gactttcaat gcatgtccaa ttctaagatc cctgataatg attccattgg
    58021 ttaccgatac tgcgaaggcg tccatcgaaa ggccaaccgc aatcaaaaca attgatagta
    58081 tatccatgtt tctcctccct taatatactc tcattttctt taaatatctt catgtttcgt
    58141 agatttt
//
LOCUS       DAOOAB010000011        53945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_731_length_53945_cov_15.467675, whole genome shotgun sequence.
ACCESSION   DAOOAB010000011 DAOOAB010000000
VERSION     DAOOAB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 53945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53945
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_53945_cov_15.467675"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PKU88_04030"
     CDS             complement(<1..510)
                     /locus_tag="PKU88_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPX68483.1"
                     /translation="MPFDGSVVSSIVHELQNKLLNGKIEKVHQPEKDELIMNVRGYGD
                     SFKLLLSASSAYPRVHLTEENKSNPASPPSFCMLLRKHLTGGRIVSIRQPEFERIIEI
                     DIDSTDELGYSTHKTLISEIMGRHSNIIFIDKAAGRIIDSIKRISFEISSVREILPGG
                     YYEYPPSAGK"
     gene            906..1781
                     /locus_tag="PKU88_04035"
     CDS             906..1781
                     /locus_tag="PKU88_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPX68484.1"
                     /translation="MIKSMTGFGRGEFTQCTSTFSVDVRSVNHRYCDVSVRMPRAMSA
                     LEEKVREFVSERISRGKVDVYINYSTFGQNARVKLDTNLAKAYVDSLNTLKEIFEIND
                     DINLSLLSRFPDIMALETVEQDMEELWLILKDALEMAFNALVEMRKREGERLKNDLLE
                     KVAVIKDYVEGIKEKSSGIVDEYKNKLYDRIQELIKDIPIDENRLLTEVAVFADKASI
                     DEELVRLGSHMDQFKKALNFNGPVGKKLDFIIQEMNREVNTIGSKITDLEILNNVISI
                     KTEIEKIREQVQNIE"
     gene            1794..2072
                     /locus_tag="PKU88_04040"
     CDS             1794..2072
                     /locus_tag="PKU88_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPX68485.1"
                     /translation="MGIKLINIGFGNIVSANRLVAIVSPESAPIKRIIQEARDRGMLI
                     DATYGRRTRAVIITDSDHIILSAVQPETVAHRLVDREAEDNEEVMAEE"
     gene            2090..2701
                     /gene="gmk"
                     /locus_tag="PKU88_04045"
     CDS             2090..2701
                     /gene="gmk"
                     /locus_tag="PKU88_04045"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004813319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPX68486.1"
                     /translation="MGNRGLLIVIAGPSGVGKGTICRELLRNYGENLELSISVTTRKP
                     RDGEAEGIHYFFRDMEEFFKMVENGELIEFAKVHDNYYGTPKRYVMEKLEEGKNVILE
                     IDVQGAMNVKKIFNEAVLIFVIPPTFADLKKRIEGRGTETKKDICKRLKNAYDEINHA
                     VNYDYIIVNDDIKDAAEKINCILIAEKSRLSRCNIDFEKFREE"
     gene            2703..2927
                     /gene="rpoZ"
                     /locus_tag="PKU88_04050"
     CDS             2703..2927
                     /gene="rpoZ"
                     /locus_tag="PKU88_04050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPX68487.1"
                     /translation="MIYPTLMSLLEKVDSKYTLVVAVAKRARQLVDGQTKLTNVNSNK
                     PVTIAINELYEEKITYERTKPVKISGDQNA"
     gene            2920..4116
                     /gene="coaBC"
                     /locus_tag="PKU88_04055"
     CDS             2920..4116
                     /gene="coaBC"
                     /locus_tag="PKU88_04055"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPX68488.1"
                     /translation="MLSGKKIVLGVSGGIAAYKACDIVSRLKKLNADVHVIMTASAEK
                     FVTPLTFQSLSLNQVAVDMFDTPNYWEIEHISLAKLADVIVIAPATANIIGKLANGIA
                     DDMLSTTIMATKAKVLIAPAMNTNMYENPVLQRNIKLLSELGYIMIPPTEGRLACGDV
                     GKGKMAEPAVIEKSIIDLLNPGQDMAGRTVLVTAGPTREAIDPVRFISNNSTGKMGYA
                     IARQAALRGAKVYLVSGPTILDTPIGVERYNVISAEEMYSKVMELFPKCDIVIKAAAV
                     ADYAPMTAYSQKVKKSGNQLELKLKKNPDILFELGKIKGDKILVGFAMETENLVENAA
                     EKVKKKNLDFIAANDLNEAGAGFAAETNAVKLIDREGNIENIPLKMKDEIADIILDKI
                     VNLKRT"
     gene            4178..6616
                     /gene="priA"
                     /locus_tag="PKU88_04060"
     CDS             4178..6616
                     /gene="priA"
                     /locus_tag="PKU88_04060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPX68489.1"
                     /translation="MGKMYAEVVVSNKCKETDRTYSYLVPENMKESIRVGSRVIVPFG
                     MGNKQIEGYVVGLKDSIDFKPSKIKSIGRLLDEKPVMSPKLVELAKWMKEKYLCYNID
                     AIQAIVPSPVRTKSSYSIELCGCEQNLEKLEKLNSNIILEIVEFLEENSGSAKLEEIR
                     EYFSDRKIDYYIKKLIEAEVVKKVQIIGSKIGEKKQKRLYLSEDKNIQIKKTASKQRE
                     VYELLLKCPGISPAKLKEACGDCGSAVKAMEKKGYVRIEETDEYRRVCSKTYAEKRHE
                     LTQDQNKALSVIRNCFSNGKDVLLFGVTGSGKTEIYLELIEEYISKGKDSIMLVPEIS
                     LTPQMVSRFKNRFGDNIAVMHSSLSEGEKYDEWRKIRDGIVKVAIGARSAVFAPFKNL
                     GIIIIDEEHEHTYKSGIKPKYLTREVAEKRCRLEGANLLLGSATPSIETYYRAQTGEL
                     GMAELGSRANGSPMPEVEVVDMREELESGNRTIFSRSLTDAILDNLKNKEQMILFLNR
                     RGHSTFVSCRQCGIVMKCPRCSISLTYHMKNEKLVCHYCGYERDNPAICPKCKSKNIK
                     YFGVGTQKLEKEFQRKFSAKDVLRMDADTTTKKNSHQEILDKFKRKEANVLMGTQMIS
                     KGLDFPDVTLVGVIAADTSLNLPDFRSAERTFQIIAQVSGRSGRGNKTGRVIVQTYSP
                     EHYSIEYARCHDYKGFYNKEVMLRKELGYPPFSSIANIMISGTAEEEVIAYANEISKF
                     LDEALKGYKDAEKLGPAAAAISRIKNRFRWQILVKSEREETLRKVLADLSEACGEGRS
                     NVSISIDLNPQSML"
     gene            7022..7477
                     /gene="def"
                     /locus_tag="PKU88_04065"
     CDS             7022..7477
                     /gene="def"
                     /locus_tag="PKU88_04065"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPX68490.1"
                     /translation="MAIRTIRKDGDEILRKISRKVEVIDDRILTLLDDMKDTMKKAEG
                     VGLAAPQVGVLRRIVVIDVGEGLIELINPVIVYESGEQFEMEGCLSIPGIKGKVKRPE
                     KVIVRAQNRKGKTFEMTGTELLAIAFCHEIDHLNGILFTDKAIITDKED"
     gene            7500..8435
                     /gene="fmt"
                     /locus_tag="PKU88_04070"
     CDS             7500..8435
                     /gene="fmt"
                     /locus_tag="PKU88_04070"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPX68491.1"
                     /translation="MRIVFMGTPDFAVPCLRELIAHKHEIAAVVTQPDRPKGRGNKLA
                     ESAVKIFARESGISIFQPEKIKTGEFVQILRELAPEAIIVVAFGQILSQEILDIPPLG
                     CINVHASLLPKYRGAAPINWCIINGDKTTGVTAMYMDKGMDTGDIILKKEIEIGEDEN
                     AGELHDRLSELGASALSETMELLIEGKIERTPQNNEEATYAPIMTKELGRIDWSRDAK
                     DIRNLIRGTYPWPGAFSSYNGKIFKILRSQAMDDNTDGKNYGCIGSVHKDSIVVNCGG
                     GSLKILDLQFENEKPMSVEAYLRGHTIIAGTILGK"
     gene            8533..9222
                     /locus_tag="PKU88_04075"
     CDS             8533..9222
                     /locus_tag="PKU88_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPX68492.1"
                     /translation="MFYGYGLDPTFILILPAIILAAYAQSKVSSTFNKYLRVRNSYGH
                     TGYEVARRILDLNGLRDVPVELVRGRLSDHYDPRTRVLRLSQDVYNSNSLASVGVAAH
                     ECGHAIQHSEGYAPLVVRNAIAPVASFGSQAAWLFIIAGFIFSWVNLIDVGILLFSAA
                     VAFQVVTLPVEFNASSRAIALLEGNGLVPSDEIGPAKEVLNAAALTYVAATFAAVMQL
                     VRLLVLRGRRD"
     gene            9391..10737
                     /gene="rsmB"
                     /locus_tag="PKU88_04080"
     CDS             9391..10737
                     /gene="rsmB"
                     /locus_tag="PKU88_04080"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPX68493.1"
                     /translation="MKKIDLARDAAVRIVHRVLKEGAYSNIALKEELDKDRLLRVDKA
                     LVTEIVNGTLRNLTRIDWIISRFIKMKSIDPWIEDIIRCGIYQILFLDRIPDSAVCNE
                     SAELARKYGHEGTVRLVNGVLRNISRNKEKLEYPDKSENAASYLSVFYSHPEWIVKKW
                     LKDFGMEFTEALLKANNEKPPFTIRCNRLKIEKKGLLAILAEEGIEYTDGSYNAEAIH
                     IRGTSSIEAKESFRKGYYQVQDESSMLVAHIVDPKPGDKVLDICSAPGGKTTHMAELM
                     DNKGEIVARDIYRNKLRLVEENCDRLGISIVRTELFDAMVKDQGSIGKYDRVLLDAPC
                     SGFGVIRRKPDLRWKKDAKNLKELVDMQRRILELASEYVKPGGKLIYSTCTINRAENL
                     DVVRGFLSNNPQFQMESIIGQVPEKLENDSAHMGYLVLYPNVHGTDGFFIAKMEKR"
     gene            10744..11796
                     /gene="rlmN"
                     /locus_tag="PKU88_04085"
     CDS             10744..11796
                     /gene="rlmN"
                     /locus_tag="PKU88_04085"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPX68494.1"
                     /translation="MPDKKNIKDLGFDELEIYIKEHRLPGFRAAQVFEWIYKDTASFK
                     DMTNLPKNLIEQLEWDFHIGRAYIAAMQHSKTDNTRKYLICFEDGNAVECVLMDYSYG
                     KTLCISTQFGCRMSCEFCASTTGGLIRSMTSGEMLEEVMAVSRDIGERIGNIVLMGTG
                     EPFDNYDQVMKFIRTINHPKGLNIGQRHITLSTSGIVPRIYDFADEKLQCTLAISLHA
                     ADDEARSRIMPINRKYNIEKLMEACRYYINMTNKRITFEYALIKDVNDSVKDAHKLGT
                     LLKGMLCHVNLIPVNKVEGKNFSKASKERINSFKAALEGFGIETTVRRELGSEIDAAC
                     GQLRQKHINRQGVTNI"
     gene            11897..12664
                     /locus_tag="PKU88_04090"
     CDS             11897..12664
                     /locus_tag="PKU88_04090"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPX68495.1"
                     /translation="MRSAGRTDIGLVRKVNEDRFLCEKLEDIDSTYLYIVADGMGGHN
                     AGEVASSMAVQEVASYIKNSIGTLMAGDEEIKDLVRNAIMYANEKIYKTSILKSNCLG
                     MGTTLSMVLAKDSSIYIGHVGDSRIYLIRGKDISRLTEDHSLVAELVKRGTIKPEDAS
                     NHPQKNVITRALGTEYSIEPDVYQYDMQSGDLILICTDGLSNAVCEEDIAGVMNAASD
                     LNGACELLIEKAKEKGGYDNITAVAIQVCKGGEDNDR"
     gene            12654..14630
                     /gene="pknB"
                     /locus_tag="PKU88_04095"
     CDS             12654..14630
                     /gene="pknB"
                     /locus_tag="PKU88_04095"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HPX68496.1"
                     /translation="MIGRVLGDRYEILEKVGGGGMALVYKAKCRLLNRYVAIKVLRSE
                     FTEDEEFVKKFRREAQSAASLSHPNIVGIYDVGTENNSYYIVMEYIKGQTLKELIKSK
                     GTLGVEYSTNIAIQICYALEQAHKNNIVHRDIKSHNILIREDNSVKVTDFGIARAVSS
                     STITNTGNVIGSVHYFSPEQARGGYVDEKSDIYSLGVVMYEMLTGRLPFEGDSPIAVA
                     LKHIQEEPEPPSGINSRIPKAIEAIIMKCMEKEVSKRYNSASEIINDLRQSLVMPNGD
                     FVKKNRYTDESTRVLEPIKIIDKEAAVDIIEEPESKELNEGKEEKILIDDYSVDPIPK
                     VAEGKDKPVQNKRNVKLTIVAVFGGLLLALLLGGGILFVNSLFTVKDVEVPDIRNNSE
                     EEAREKLSALSLILEVTERVFNKDIPEGSIISQNPKPNEKNKITNPVKVVVSKGPRKV
                     VVPKLEGESYDKVKLILEGEGLVEGSFDQEYSEYPNGFVIRQSIAPGISVDEGTTIDY
                     VISIGPEKFIMPSYLGADIEDVKTDLIIKDLILGNISYDSNSEQPADTVLEQSIRPGT
                     EVSRKSVVDFVVNNYKPEPVPSSFKLRFTLPPELESMKVTVYKVQNEISSRIYESIHK
                     STDSPLEIPVSGVGNVIFEVYINDSLHESVAHNF"
     gene            14645..15526
                     /gene="rsgA"
                     /locus_tag="PKU88_04100"
     CDS             14645..15526
                     /gene="rsgA"
                     /locus_tag="PKU88_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPX68497.1"
                     /translation="MQKGVIVKGIGGFYYVDTGEGVYECRARGKFRLKNITPLIGDHV
                     EIDVDAGSLQGYIISILERKNQLIRPTVANIDQAVIVFAAKKPDINMVLMQKFLVYSE
                     YKNLNVIVCINKIDLDPDGHYLDVVRMLEGIPYKVITTSAKTKVGIEQLQEILKDKVS
                     VFAGPSGAGKSSLLNCIQPGLKLKIGDLSKKIDRGTHTTRHAELIKLDEGGMVADTPG
                     FTSLDIGELELSKLESCFPEFESHKNCQFVNCIHDKEPRCGIREAVLEGKIHNIRYDF
                     YISLLNELKSTGGRNYK"
     gene            15523..16182
                     /gene="rpe"
                     /locus_tag="PKU88_04105"
     CDS             15523..16182
                     /gene="rpe"
                     /locus_tag="PKU88_04105"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPX68498.1"
                     /translation="MIKIAPSILSADFANLERDIGTVERAGADWLHVDVMDGHFVPNI
                     TIGPAVVKSLRAKSSLVFDVHLMIDNPELYINDFAEAGADIITVHAEASVHLHRLLQM
                     IKAKGLKAGMALNPATPVNVLEHIIEDLDMVLIMSVNPGFGGQRFINSAYEKISKVRS
                     LIEKKNLSVDLQVDGGIGPDNIRQVVMSGANIIVAGSAIFNSGNIPETIRIMKEAGIS
                     K"
     gene            16182..16814
                     /locus_tag="PKU88_04110"
     CDS             16182..16814
                     /locus_tag="PKU88_04110"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPX68499.1"
                     /translation="MKTVIISGGEIKDYEFCSGIVRSADRVICADSGTRHAMNMNLTP
                     DVIIGDMDSSPAQYIDFFRKKGVEVIQYPKEKDKTDTHICVEYALDFSTEIILLGATG
                     SRIDHMIANISLLMLGIRRNIPMSIIDNKNCIRMIGDSITLKGNKGEFFSLIPYTGKV
                     EGLSTRGAHYELENAVMELGNPYGVSNYFEEETVSITIKKGFLLVIKSED"
     gene            16941..17096
                     /locus_tag="PKU88_04115"
     CDS             16941..17096
                     /locus_tag="PKU88_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68500.1"
                     /translation="MRYTYKRYAGCLCIILSVFLVSRFMPLFLWCFAVAAAVAMIGFL
                     LYNCFFM"
     gene            17114..17266
                     /locus_tag="PKU88_04120"
     CDS             17114..17266
                     /locus_tag="PKU88_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68501.1"
                     /translation="MSLLKRKKLTGFLAFCIGLGILFAVLVPAIGWVMFSAICLISIG
                     IFLIKY"
     gene            complement(17331..17522)
                     /gene="rpmB"
                     /locus_tag="PKU88_04125"
     CDS             complement(17331..17522)
                     /gene="rpmB"
                     /locus_tag="PKU88_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPX68502.1"
                     /translation="MARHCEVCGKGMISGNKVSHSNRHSRRIWLPNVRTVKAVVNGTP
                     QRLHVCTRCLRSGKVQRAI"
     gene            17819..18169
                     /locus_tag="PKU88_04130"
     CDS             17819..18169
                     /locus_tag="PKU88_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPX68503.1"
                     /translation="MSAIYKTQYGEVNITDDVLATISGLAATECYGLVGMASKNAKDG
                     LVELLKRENLAKGVKVTTEGEEIIVDLYIVVEFGTKISTIADNIISKVKYTVENLTGL
                     KVKKVNINVQGVRV"
     gene            18192..19829
                     /locus_tag="PKU88_04135"
     CDS             18192..19829
                     /locus_tag="PKU88_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPX68504.1"
                     /translation="MSTKNIDGIQLRNMFLSGANNLENNKGIVNSLNVFPVPDGDTGT
                     NMSLTMHLAVKEIIAVKSIEIQSVANAAANGSLMGARGNSGVILSQIWRGFAKALKNQ
                     KSMDVNLLANALTEGSNTAYKAIMKPTEGTILTVIRETAEYAMKVSENFEDNVLFWEE
                     IIEKANSVLRRTPEMLQVLKNAGVVDAGGKGLIYIFEGMYQYLKTGEMIELKAAADDE
                     EHDRPLFKEEEIVFGYCTEFFIKGRKLGLEKFRDEILPIGDSLLVVGDENLLKVHIHT
                     NNPGRVLELAMAQGELSRIKIDNMREQHNELLFNKEEHKAAEEAEQAPKPMERYGTIA
                     VAMGEGISSIFKDLGANEIIEGGQTMNPSTEDILNSVNKINAETIYIFPNNSNIILAA
                     NQAKSMSDKNVIVAATKSIPQGISALMALNHDKEIEENNKKIEKAITNVKSGLVTYAV
                     RNSNYDGINIEEGNVLGMVEGKISGVGNDILEVSHKVLDDMVDEDSSLISIYYGSEVN
                     EDEALKLKEEIGELYNNCEIELHFGGQPLYYYILSVE"
     gene            19847..21907
                     /gene="recG"
                     /locus_tag="PKU88_04140"
     CDS             19847..21907
                     /gene="recG"
                     /locus_tag="PKU88_04140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPX68505.1"
                     /translation="MNYTEDSITNLKGIGPVKARLFNKLGLYTIGDLLEYFPRGYKDK
                     SEITKIRDIMPDESCTVRVRAAGKAQEGRTRSRLIITKLTVFDDTGRLNAVWFNNRFI
                     KNAFREGDEVLMHGKVKRIGKDLVMEVPEYERLSDSESINLMRITPYYSLTEGLSQKD
                     IRKAVYAALKHAEKQADLFPEELRRKYGLAEINFCISNIHYPESMDKLKLARKRLIFE
                     EFFMLQSGLIHIKKLNSAGQKGIRFKKTSEIKEFIEHLPFKLTSAQERVFEEVSKDME
                     GSGIMNRLVQGDVGSGKTVIAALALFKCVRSGYQGTMIVPTEILAEQHMETLTNLLKN
                     TGVEIALLKGGMTGKEKQKVLEGIKEGKIDVVVGTHAIIQDSVSFDKLGLVITDEQHR
                     FGVRQRALLSAKGENPDMLVMTATPIPRTLSLIVYGDLDVSIIDEMPPGRKKVETYFI
                     DSAKKERLMNFVKKGLDEGRQAYFVCPLVEESDKLELKSAIEYSEELKNKYFRNYNVG
                     LLHGRMKADEKNEVMYRFREKEIHILVSTTVIEVGVNIPNAAYMVIEDADRYGMAQLH
                     QLRGRVGRGGHQSYCILISDTGNEIAQERLKYMTKTENGFEVAEKDMEFRGSGEVLGQ
                     RQHGLPGFKLADVFRDAKLLKLSKEAVEYIYENNKLSNEDYKKMKEILDQRFQKMLDE
                     ITLN"
     gene            22073..22282
                     /locus_tag="PKU88_04145"
     CDS             22073..22282
                     /locus_tag="PKU88_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPX68506.1"
                     /translation="MPRNTTSNRTLIPEAKAALKTFRDQIATELGTDLTGYNGDKTSW
                     ECGKVGGEMVRRMIEQAERSMSGRR"
     gene            22771..23346
                     /gene="rsmD"
                     /locus_tag="PKU88_04150"
     CDS             22771..23346
                     /gene="rsmD"
                     /locus_tag="PKU88_04150"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPX68507.1"
                     /translation="MRVIAGEYKGRRLDPIEGRDIRPTSDMVKESLFNILGDAVIDSV
                     FLDLFSGTGGIGIEALSRGAKHVVFIDSGIKSIKVLRGNLNRLNIKDCAEVYNTDYGT
                     AINKLHEHGKSFDLIFIDPPYSMGMAESALKEIDNKNILTQSGLIVVEHDSKEDMPQK
                     VGKLFLYRFKKYGNTILSFYSIDEQSQQKEE"
     gene            23349..23825
                     /gene="coaD"
                     /locus_tag="PKU88_04155"
     CDS             23349..23825
                     /gene="coaD"
                     /locus_tag="PKU88_04155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPX68508.1"
                     /translation="MKIAVYPGSFDPATNGHIDIIKRSGALFDRVIVAVLSNPSKNPI
                     FSVEERVDILKRTCGDIKNVEIDSFSGLLIDYCKSKNANVIVKGLRAVSDFEYELQMA
                     HMNKKLNQEIETVFIMTSSRYSYLSSSLVKEVAGFGGCIRGLVPEIIEKEIYERLK"
     gene            23898..24434
                     /locus_tag="PKU88_04160"
     CDS             23898..24434
                     /locus_tag="PKU88_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPX68509.1"
                     /translation="MEVMLLLDALEDILDKAPNLPLSSKSMINKEELQEIIKEIRIKL
                     PDEIKQAQWIKEERQKILIEAKKEAENLRKECEEKLNKVKEERQRILAEAEKEAEILK
                     KEADKKVQELIDESDIIKKANEQAKELIIAAQIDAKKIRLGSKAYADELLAELEKHTE
                     KIIETIRNNRDELKNYKS"
     gene            complement(24480..25673)
                     /gene="ylbJ"
                     /locus_tag="PKU88_04165"
     CDS             complement(24480..25673)
                     /gene="ylbJ"
                     /locus_tag="PKU88_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HPX68510.1"
                     /translation="MKKGTISTFAYSFIFCILVTFIIRYPGEAFAASLDGLKLWFNVV
                     CPALLPFFICVEILIGLGIVRLFGSAFRPLMGPVFNVPGEGSFDFFMSISSGYPVGAK
                     ITAELYSKNLCTLAEGQRLLSLCSTSGPLFIIGAVATGILNNPALGSFIAIAHYLSAI
                     TIGFAMRFYGKENPRIREKYISNPIAEMMEYRRRDGRTFGMLMRDAVKNGIDLILMIG
                     GFIIFFSVITGMLRISGFLEWFSLSLSRAIPLKSITPDIISSLLIGLLEVTNGAKSCA
                     SLRVPLIYKAAITSFMIGFGGLSVNAQVMSIISGTDLKLGIYFITKLFQGIAAAFYSY
                     VLVAWFSDLQVFNLPAESFVQNTILEYSFLRVLVTSAANMLIILLFMSAVHIKGTWGR
                     FSCPK"
     gene            complement(25753..27003)
                     /locus_tag="PKU88_04170"
     CDS             complement(25753..27003)
                     /locus_tag="PKU88_04170"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HPX68511.1"
                     /translation="MMKTVGIVSEYNPFHNGHKYHIRAAKEACGADYAVCIMSGSFVQ
                     RGEPSIFDKWSRSKSAVMNGADLVIELPVVYACQPAEIFAAGAVKILDSLGVVDHICF
                     GSELGDTGALHRLANLLQNEPEAFKHLVKGQLGRGVSYPKAVSNALSHYASDDIHYAS
                     DDILKNPNNVLGIEYIKALKSLNSSITPVAVKRIVSGYNDNTIAGSIASATAIRNEIK
                     EGGISDKVRMSLPGSSIDIISVNLRMNRHPVYMNDFSDILLYKLRTMEKSELLKYPNV
                     KEGIENRLKKYAAASTNCEDLIEAVKTKRYTRTYIQRLLCHVLLDIKNADISMFKSRE
                     CPSYVRVLAFNNNGKKLIKMIMDCCPYPVISKVADFRCTDKRIIKMFSYDIQATDIYN
                     LAYRTSEFRKAAEDYFTSPYYHRE"
     gene            27209..28414
                     /locus_tag="PKU88_04175"
     CDS             27209..28414
                     /locus_tag="PKU88_04175"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPX68512.1"
                     /translation="MKILVINCGSSSLKYQFIDMENEEVIAKGLAERIGIDGSVLNHQ
                     PKGFDKIRIERPMPTHKEALQTVVDALRDKEYGVIKDMGEITAVGHRVVHAGEKFAYS
                     VLLKEEVMDALKECISLAPLHNPPNIMGIEACKQIMPEVPMVGVFDTAFHQTMPKKSY
                     IYPIPYEYYEKYRIRRYGFHGTSHKYVAERAAAMLGKPVEELKIITCHLGNGASITAV
                     KNGISVDTSMGFTPLEGLAMGTRSGNIDAAIIKFLMEKENISVAKADEILNKKSGVLG
                     ISGVSSDFRDIEEAANKGNERAQLALDVYVHYVKKYIGAYAAVMNGVDAIVFTAGLGE
                     NSASMRSSICKDMSFLGVELDEAKNNVRGKETDVSKDGSKVRVLLIPTNEELMIARDT
                     AELAGKAKK"
     gene            28560..29066
                     /locus_tag="PKU88_04180"
     CDS             28560..29066
                     /locus_tag="PKU88_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPX68513.1"
                     /translation="MELNFAGISKSEGVKERFEIIEYPIDSTIVFCGENLNIVSPITV
                     KGSAVNYEGKISVDLTITATVERTCSRCLEIFTEEVQADSNYVFVKDIADDKEDYYIY
                     RGDKADITDLVIGDIAAKLAMKPLCRANCKGLCTVCGKNKNSIDCQCKSEEVDPRMQA
                     LSKLLDRK"
     gene            29080..29259
                     /gene="rpmF"
                     /locus_tag="PKU88_04185"
     CDS             29080..29259
                     /gene="rpmF"
                     /locus_tag="PKU88_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPX68514.1"
                     /translation="MGLPKRRHSQSRRDKRRANWKLALPGLVACPQCHEPKLPHRVCK
                     SCGYYKNRTVIKTEE"
     gene            29572..30135
                     /gene="fapR"
                     /locus_tag="PKU88_04190"
     CDS             29572..30135
                     /gene="fapR"
                     /locus_tag="PKU88_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HPX68515.1"
                     /translation="MKQGGISKKERQASLLDRIKTDPFLTDEELAESFNVSIQTIRLD
                     RLELGVPELRERIKSVAEDNYTKVRSLHGKEIFGELVDLNLGVSGISILETDNNLAFE
                     KTNVVKGQYIFAQAESLAIAVIDANVALIGVANIKYKNPVFAGDKLIAKAEVVRTRGN
                     KYFVWVFIRVKQAEVFRGKFILVSFSN"
     gene            30286..31302
                     /gene="plsX"
                     /locus_tag="PKU88_04195"
     CDS             30286..31302
                     /gene="plsX"
                     /locus_tag="PKU88_04195"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPX68516.1"
                     /translation="MRIAVDVMGGDNAPGAIIEGSILALKEYGTELYLIGLRDEILKY
                     LGEEYKNHQKVNIIEASEIINADEAPVSAIKQKKDSSMVIGLKLVKEGQCDAFVSAGS
                     TGAFLAGSLLKVGRIKGIDRPALSPLLPTLKGSCMIIDAGANADSKPKNLQQYAVMGS
                     IYMEKVMGIKKPKVGLLNIGAEASKGNELTKQSYTLIKNTGVNFIGNTEARDIISGVC
                     DVCVCDGFAGNVLLKTTEGVAMTLFKLLKNEFMKSLKTKLGAMLLMDSLKNIKKSFDY
                     TEVGGAPFLGINGIMIKAHGSSDAKAIKNAIRQAKMLYDNKCLDTIMEEIIKLGVEDT
                     DEQA"
     gene            31289..32296
                     /locus_tag="PKU88_04200"
     CDS             31289..32296
                     /locus_tag="PKU88_04200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPX68517.1"
                     /translation="MNKLRNAGIIGTGSCLPDKIVTNQDMEKTVDTTDEWIRTRTGIS
                     ERRMTDENTATSDLATIAAMRALASANLSPEEIDLIIVSTVTPDMMFPSTACIVQKNI
                     GAVNAAAFDLEAACAGFLYGLSIADSFVRAGSFNKILVIGAETLTKILDFTDRNTCVI
                     FGDGAGAAIVSEVPEGYGIMSTYIGADGRGGDLLKVPAGGSRIPSSVESVQNRLHYVK
                     MDGKEVFKFAVKIMGDAAEIALGKCGLTKEDVDFFIPHQANIRIIEASVKRLKIAHEK
                     VYINLDKYGNMSSASVAIAIDEANRKGLLKDGDTIVLVGFGGGLTWGSAVLKWKSIRR
                     D"
     gene            32298..33248
                     /gene="fabK"
                     /locus_tag="PKU88_04205"
     CDS             32298..33248
                     /gene="fabK"
                     /locus_tag="PKU88_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HPX68518.1"
                     /translation="MFKTSICEILDIEYPILQGGMAWVATAELAAAVSNAGGLGIIGA
                     GNAPGSYVRDEIRKVKKLTDKPFGVNIMLLSPFVDEVIDIVYEEKVPVITTGAGNPGK
                     YIARFKNIGTKVIPVVPAVALAKKMEQEGVDAVIAEGTESGGHVGELTTMALVPQVID
                     AVKIPVIAAGGIADGRGLVSVLALGAQGVQMGTIFVCASECTAHENYKKAIIKAGDRG
                     TVVTGKATGHPVRILKNKLAREFEKLEKASAAVEEYEKLGVGRLRAAVVEGDADYGSV
                     MAGQIAGLVKEIKPAKDIIEDILNEANEVISSINGLVKEE"
     gene            33250..34200
                     /gene="fabD"
                     /locus_tag="PKU88_04210"
     CDS             33250..34200
                     /gene="fabD"
                     /locus_tag="PKU88_04210"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPX68519.1"
                     /translation="MPKIACIFPGQGAQYPGMGKELAQNYKEAMNIFDLASERLGIDM
                     KKLCFEGDEEELKKTENTQPSILTVSIALLEVLKLKGIEPQIAAGLSLGEYSALVASK
                     AISFADAVALVKKRGKYMQEAVPAGEGTMAAIMGIERESVMECLKMASGYGVVEAANF
                     NCPGQVVIAGHTSAVEKACSILKERGARKAIMLPVSAPFHSSLLKPAGEKLAAELENV
                     NISDNELPVVSNVNAQVIMNKYEIKRCLIEQVSSSVLWEDSIRRMIDLGVDTFIEVGP
                     GRTLSAFVKKIDKSLYVHNVENIETMENTIVSIRERLECN"
     gene            34191..34934
                     /gene="fabG"
                     /locus_tag="PKU88_04215"
     CDS             34191..34934
                     /gene="fabG"
                     /locus_tag="PKU88_04215"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPX68520.1"
                     /translation="MQLKGKTAVVTGGSRGIGRAIALELASCGANVVVNYTRNSKAAD
                     EVVAEIEAMGSSGMAVKADVSIASEVENLVNEVLKTFGSIDILVNNAGITRDNLIIRM
                     TEKEFDEVINTNLKGTFICTKAVSRVMIKQKSGKIINVSSVVGIVGNAGQSNYAAAKA
                     GLIGFTKSMAKELAKRGINVNAVAPGFIQTDMTSVLPDNVKEEFLKSIPLMRIGKPED
                     IAKTVLFLVSEYSDYITGQVINIDGGMVM"
     gene            35079..35306
                     /gene="acpP"
                     /locus_tag="PKU88_04220"
     CDS             35079..35306
                     /gene="acpP"
                     /locus_tag="PKU88_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPX68521.1"
                     /translation="MIFEKVQEKVAEQLGIEPEEITLESSFINDLGADSLDIVELLMA
                     LEEEFDIEIPDEEAEKLSTVGDVVEYIKSNK"
     gene            35404..36645
                     /gene="fabF"
                     /locus_tag="PKU88_04225"
     CDS             35404..36645
                     /gene="fabF"
                     /locus_tag="PKU88_04225"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPX68522.1"
                     /translation="MNKRRVVVTGIGVISPIGTGREKFFKSLKEGVSGVGHITRFDTE
                     GFDTKIAAEVKDFDPSGYFDRKECRRMDRFSQYAIAASKMAAEDSNINIESLDSSRFG
                     VCIGSGIGGMETMEAQYDILREKGPGRVSPFMIPMMISNIAAGNISIALNAKGPSITV
                     VTACASATNAIGEAFKIIQRGDAEIMVTGGTEASITPLSLAGFCSMKALSTRNDDPKA
                     ASRPFDKDRDGFVMGEGAGMLIIEELEHALKRGAVIYGEIAGYGATGDAYHITAPAPD
                     ADGAYRAMLMAVNDAGINACELDYINAHGTSTDLNDKLETLAIKRLLGEEAKKVAVSS
                     TKSMTGHLLGAAGAIEAIACLMAINEGVIPPTINYTTPDEECDLDYVPNKAVNRKVDW
                     AMSNSLGFGGHNASIILKKYQ"
     gene            36760..37479
                     /gene="rnc"
                     /locus_tag="PKU88_04230"
     CDS             36760..37479
                     /gene="rnc"
                     /locus_tag="PKU88_04230"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPX68523.1"
                     /translation="MSVIDEKRLKQLNEFESIIGYSFKNISILNNALTHSSYVASRSD
                     HHEHNERLEFIGDAILDMVISLYLYKKCKNLTEGSLTRFRAGIVCEQSLYNASNNLKL
                     GEYLLLSKGEENTGGRKRVSILADAFEAVIAAIYLDGGIKKAKEFIMKNLETTIKNAV
                     ENKLFSDYKSFLQEYVQKNNLGKLSYKLVREEGPDHDKTFEVALYLNNGIIGRGEGRS
                     KKDAQQSAARDAIAGLGVRNV"
     gene            37472..38542
                     /locus_tag="PKU88_04235"
     CDS             37472..38542
                     /locus_tag="PKU88_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX68524.1"
                     /translation="MFKKHYIIPIFVPHKGCPHDCIFCNQKKITGQIEKVTEKDVKDK
                     IEDYLDMIHEGGSHIEVAFYGGSFTAIPLEYQEQLLDAAFVYVKAGKITGIRVSTRPD
                     CIDARISENLRKYGVRTVELGVQSMDEEVLKLCNRGHSPGDVTNAVGILKEFGFSVGV
                     QMMIGLPGDTEEKAVKTAKALIALKPDIARIYPALTIKNTYMEEMFIRGEYQPLTLDE
                     AVELSKKLLILFESNGINVIRIGLQPTENINSGKDVVAGPFHPSMRHLVESLIYRDML
                     AYLLEKEGEKKKITLGINPKNVSELVGHKKSNIDYIKSKFFIDKIEIFQDNNVEAGTM
                     VLNNGKNSVIMSKKYYYSILSK"
     gene            38611..38871
                     /locus_tag="PKU88_04240"
     CDS             38611..38871
                     /locus_tag="PKU88_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HPX68525.1"
                     /translation="MEVLKVSAHSQPKSVAGALAAVLRDRATAEIQAVGAGAVNQAVK
                     AIAITRGFVAPNGIDLVVVPAFSEIQIDGEDRTAIKFIVEPR"
     gene            39015..42575
                     /gene="smc"
                     /locus_tag="PKU88_04245"
     CDS             39015..42575
                     /gene="smc"
                     /locus_tag="PKU88_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPX68526.1"
                     /translation="MYLKQLEVHGFKSFAEKIELSFGNTINAIVGPNGSGKSNISDAI
                     RWVLGEQSVKALRGNKMEDVIFAGSDSEKALGFAEVSLCIDNSDRKIPVDYTEINIMR
                     RMYRSGESEYFINKTSCRLKDIYELFMDTGIGRDGYSIIGQGRIDEILSNKSEDRRNV
                     FEEAAGIVKYKTRKHEAEKKLEQTNQNIVRLGDIIEELESQLGPLKEQAEVAKRYRSI
                     MEELKSIEINIMLNSIDKFRSRLSNAKQEAENIQSLIDAKNSSARQLEHKQNELKRIL
                     NDIETSIDMLSEDIHHLENSKEKMEGESNVLQVRINNLEENTLKLGKSGEELENELTE
                     SRQGIEQNRLKLAELNTAYIEVYGKINERTADFESNFKAINDLESDLSDKQQKFISLI
                     NNYSELKSKINSTNIFIENMNKRKSQVEGDVNLLELQQQDKKKQYDKSLEELDDFGRK
                     IEDCKTQIEEIKGKKQECESEILLLEDKAGKLKAEKEAMFSRLKALDDMERDHGGYSR
                     AVKVLMAEYDKVTKGFCGVVGEMIKVPSKYVTAIEIALGGSVQSIITNTENDAKLLIE
                     YLKEHKTGRATFLPISSVKPRFFNKAEEKLFNMKGFVGIASDLIEYDLKIDSIMKNLL
                     GRVAVVEDIDAAIKLAREAGYDYKIVTLSGEVINAGGSITGGSLQSSSSSVLTRKSEI
                     EALKVKHKEKQQELDELSRKLGELKKQAADMGADIEFKTEILHSLQIDINNYRNRCDN
                     LKNEIQVIQDRISINLKDLGDLEKEYNETNKIISENKEKAEAVEKEKEAVEAQISELQ
                     EQMKQFKEVRDEYNDEITELKIKLASVEHNKKTVENSIKSMGERVTELEEEIKSNNAE
                     IERQQAEIKALSYDLEEKKTIIERTVREAEEKHKLHNDKEKEKSAICEDLSKLEADIK
                     NYSNDIAVLQADLHKAEMQHARTEIELESIEMRLLETYEMNYSKAESYRDTSLSLTWA
                     AKHCEELKNDIREMGTVNVNAIEEYEKLKERHTFLSVQVEDLSKARENLIAVIAEIEE
                     NMKLQFITEFNKINENFNIVFKQLFGGGHAELVLTDMDDVLGSGIEIIAKPPGKKLQN
                     LLLLSGGERALTAIALLFGILKMKPAPFCVLDEIDAALDDANVDRFAEFLVSFSATTQ
                     FIVVTHRKGTMECADCLYGVSMEDSGVSKLVSVRLEDKVS"
     gene            42736..43803
                     /gene="ftsY"
                     /locus_tag="PKU88_04250"
     CDS             42736..43803
                     /gene="ftsY"
                     /locus_tag="PKU88_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPX68527.1"
                     /translation="MAGLFKKLFNRGQENEQEELQAEEQEQLTDEEIEAINEEEIENE
                     ETVENEQSSIFSRLKNGLAKTRNNITGKLNGVLASFKSVDEELFEELEEILITSDVGV
                     STTLMILDKLRSRVKEQNIKDPSEVKELLKQEILAIMGESTERLGEQTPAVILVIGVN
                     GVGKTTSIGKIASKMKKSGKKVIIAAADTFRAAAIDQLEIWGKRIGVDVIKHQEGADP
                     AAVIFDAINSAKSKKADILICDTAGRLHNKKNLMEELKKISRIIEREFPEAYKETLLV
                     LDATTGQNAISQAKLFKEAADINGLVLTKLDGTAKGGIIIAIKSELDIPVKLIGVGEK
                     VKDLQEFSASDFVNALFEEDE"
     gene            43898..44254
                     /locus_tag="PKU88_04255"
     CDS             43898..44254
                     /locus_tag="PKU88_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HPX68528.1"
                     /translation="MYDKLVQIALLFDFYGQLLTEKQIEIVDMYYNNDLSLGEISEQQ
                     GISRQGVYDTLKRAERTLYQYEEKLGLVDRFLKQKQNMEKIIEMLEELITEDGYDRKN
                     IKDKINEIKEYIGNII"
     gene            44324..45664
                     /gene="ffh"
                     /locus_tag="PKU88_04260"
     CDS             44324..45664
                     /gene="ffh"
                     /locus_tag="PKU88_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPX68529.1"
                     /translation="MAFEGLAEKLQNTLKKLRGAGKLSEKDVKDAMREVKMALLEADV
                     SFKVVKEFITKVTERAVGQEVLDSLTPGQQVIKIVNEQMTELMGNTNSKIIFSPKPPT
                     ILMMVGLQGAGKTTTCGKLGLLLKKQGKNPLLVAGDVYRPAAIKQLQVVGSQVGIEVF
                     AMGDKVSPVDISKAAAAHALKNGNDVVIIDTAGRLHINEELMDELADIKENVKPTEIL
                     LVVDSMTGQDAVNVAESFNEKLGIDGVILTKLDGDTRGGAALSVKAITGKPIKFAGMG
                     EKLNELEPFHPDRMASRILGMGDVLSLIEKAQSAFDEKKAVELERKIRTQQFTFDDFL
                     DQLQQMKKMGPLSSVLEMLPGMNPSKLKGMEVDDKELVKVEAIIRSMTKKERHNPDII
                     NGSRRKRIAAGSGTSIQDVNRVMKNFEQSKKMMKQFAGMEKDMKKGKMKMPFFR"
     gene            45708..45956
                     /gene="rpsP"
                     /locus_tag="PKU88_04265"
     CDS             45708..45956
                     /gene="rpsP"
                     /locus_tag="PKU88_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPX68530.1"
                     /translation="MSVKIRLRRMGAKKAPFYRLVVADSRSPRDGKFIEEIGYYNPIT
                     EPAEIKIDAEKAKKWLDCGAQPSDTVRSLLKKSGIIEG"
     gene            45992..46219
                     /locus_tag="PKU88_04270"
     CDS             45992..46219
                     /locus_tag="PKU88_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPX68531.1"
                     /translation="MKELVEVIAKALVDHPEMVKVRETENERSIVLELTVAPEDMGKV
                     IGRNGRIARAIRTVVKSSSARLDKKVIVEIL"
     gene            46323..46826
                     /gene="rimM"
                     /locus_tag="PKU88_04275"
     CDS             46323..46826
                     /gene="rimM"
                     /locus_tag="PKU88_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907560.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPX68532.1"
                     /translation="MLEYLSIGQIINTHGLRGAVKVYPLTDDISRFKKLKEVYVEEND
                     GIVKYKVESVKFLSSAVAVKLKGIDSEEAANKLRGFYIKVDRKSAVKLPKDTYFICDL
                     IDLEVYDEKGLLIGTVKDVLQTGSNDVYVIESSGKDILIPALKDIVKKIDLENKKILV
                     EMPEGLI"
     gene            46827..47564
                     /gene="trmD"
                     /locus_tag="PKU88_04280"
     CDS             46827..47564
                     /gene="trmD"
                     /locus_tag="PKU88_04280"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPX68533.1"
                     /translation="MKIDILTLFPEMFEAVLKESIIGRALEKQLIELEFHNIRDYSDN
                     KHRKVDDYPYGGGLGMIMQCQPIFAAVEAISAKLGEKPHIVLMSPQGRTFDQAKAEEL
                     STHKYLTILCGHYEGIDERVNEALVDEEISIGDFVLTGGEIAAMAVVDATCRLIPGVL
                     KEDASYTEESFNRGLLEYPQYTRPPVFMGMPVPEILLSGHHENIEKWRRYQSLKRTWE
                     RRPDLLAESELSEEDRRLLDKIKEECK"
     gene            47712..48056
                     /gene="rplS"
                     /locus_tag="PKU88_04285"
     CDS             47712..48056
                     /gene="rplS"
                     /locus_tag="PKU88_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPX68534.1"
                     /translation="MMDLIKVIESEQLRNDIPVFNVGDTVKVHVKVKEGNRERIQVYE
                     GVVMKRQGGGLSETFTVRRISYGVGVERTFPLHSPKLDRIEVVRKGKVRRAKINYLRG
                     RVGKRAKIKEIR"
     gene            48101..48958
                     /gene="ylqF"
                     /locus_tag="PKU88_04290"
     CDS             48101..48958
                     /gene="ylqF"
                     /locus_tag="PKU88_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HPX68535.1"
                     /translation="MNIQWYPGHMAKAKRKIAEELKLVDVVIELLDARIPMSSRNPEV
                     DEIVGNKKRIIVLNKSDLADSSVNAKWLVYFNKENTRAILVNSLKGNGLKDVLSASRQ
                     LMKEKLDRLKSKGLLVKTTRALIIGIPNVGKSTFINRLAGRSVAQTGDRPGVTKSKQW
                     IKVSNELELLDTPGILWPKFEDKRAGMYLAFTGAIKDEILDVNELAQNLLQVLAEKFP
                     EKLKSRYKLEDIPEGIAPEELMKSIGRKRGCIIAGGEVDVLRASAMLLDEFRGGKIGA
                     ISLESPEDF"
     gene            49129..50070
                     /locus_tag="PKU88_04295"
     CDS             49129..50070
                     /locus_tag="PKU88_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPX68536.1"
                     /translation="MPKKNDDYISVLGGCNMDIIGFPYKNFVPEDSNPGRIKAALGGV
                     GRNIAENLVCLGIPTKLISAIGDDIYGKIILEHADNIGLDMKNSLVSNQTTTSTYLAI
                     LDEKGDMKAAVSNMDVLDEITIEFIQEKKQVIENSKICVIDTNMPTDIINYVLDNFKN
                     TDFFLDTVSGTKAQKVKNNIGAFHTIKPNRIEAEILSDIKIDSLGDMIKASEYFLSRD
                     VKRVFITLGAEGVFYNDGNVHKLIPSPKIKVINATGAGDAFLAALAFCRFKNYGIDES
                     TRFSMAAAILALSCEKTINPNISEKNVIKKMKEIGLY"
     gene            complement(50280..51415)
                     /locus_tag="PKU88_04300"
     CDS             complement(join(50280..51163,51163..51415))
                     /locus_tag="PKU88_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558188.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HPX68537.1"
                     /translation="MKKNFTEEQIISVLKQHESGVPVKELIRQHNITEQTFYRWKSKY
                     GGMEISEARCLKQLEDENRRLKELVANLTLDNQALKWVVEKKRIQPAHKKEIANELQK
                     EFKLSERRVCRLLSINRTFKRYVPAKNEENEVITKRLGELAVRWKRFGYKRLHVLLRR
                     EGFKINHKRTYRLYKEAGLTLRKRKKKSPSEKRGKPELAASPNSRWSFDFVSDATANG
                     RRIKVLTVIDEVTRECLALEVDTSITGQRVASVLNRIAFFRGYPKEVLTDNGPEFTSV
                     ALSEWAYEKKINHLFIEPGKPVQNAYIESFNGKFREECLEEHWFKNIYEAREIIESWR
                     IEYNTLRPHSSLGNLTPDEYAQKLKNENAPESAGLTFSVVQNLG"
     gene            51961..52830
                     /locus_tag="PKU88_04305"
     CDS             51961..52830
                     /locus_tag="PKU88_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX68538.1"
                     /translation="MDWVKQLNESINYIEENLVGEISYEVISKIARCSVYNFQRMFSY
                     IADKPLSEYIRNRRLTMAAFDLLNSTDRIIDIAIKYGYESQDAFTRAFKNFHNVLPSS
                     VRNEIVQLRSCPRLSFQISIKGENHMNYQIEQWPAFYVMGISHKIKTSTAFQVIPKIW
                     EEAWKDGTMQRFIENFPDYRPAGFLGIAASGQWGDSEEMNYIIAVTSHVDIHECKHIP
                     VLEGMQEFSYPAATWAIFEANGELPDATQKVYKQFYTEWLPNSGYELADMPVIECYMQ
                     ENRQEVWIAVIKK"
     gene            52918..53511
                     /locus_tag="PKU88_04310"
     CDS             52918..53511
                     /locus_tag="PKU88_04310"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HPX68539.1"
                     /translation="MGTCGKKGNIPIEAVIENITFECKLIPKGNGEYVLPLNKDIFNK
                     FSVSDEFNVKFTILKQLTRINNNSPYSKENPIRHIDSFNYLKQPSIGSCGQTCLAMLA
                     GISVQEVIKIMGSNKWQASISKVLETLDYFGLSYNIPVYTHGKKVNLPKCCIVNVSGS
                     DKNHLMVYYDGLFYDPVSRVTDDYDYENVINFIEIYD"
BASE COUNT        17927 a         9091 c        13317 g        13610 t
ORIGIN      
        1 tttgccagcc gaaggagggt attcgtagta tcctcccggc agtatttctc tgacgctgct
       61 tatttcaaag cttatccttt taatactgtc tattattctg cctgcagcct tgtcaataaa
      121 tatgatgttg ctgtgacgcc ccattatttc tgagattagg gtcttgtgtg tgctgtaccc
      181 taattcgtct gtagagtcta tatctatttc aataattctt tcaaattcag gctgcctgat
      241 tgatactata cgtcctcctg taagatgctt tctcagcagc atgcaaaagg aagggggaga
      301 tgcaggattg gatttgttct cttcggtaag atgtactctt ggataggcgg agctggcgct
      361 taacaaaagc ttgaagctgt ctccatagcc cctgacattc attattaact cgtccttttc
      421 aggctgatgt actttttcta tttttccatt cagcagctta ttctgcagct catgtacaat
      481 tgaactgacc accgaaccgt cgaaaggcat aagaacacct cctattcact ccattgggtt
      541 catctaaata ttatatcact tgagccgttt atttactatt aacaaaaaat atgtttgcgc
      601 aaaagaggaa gaatgcgggg acatggggga cgggacacgg ggacgtttcg cgtgtcccac
      661 tggaagtggg acaggggaaa cgtccccgtg tcccgtcccc gtgtccccgt gtctcataag
      721 tgcagttgat gataattaac gaatttatca gaaaatagga gcaatatcaa ggaaatcaaa
      781 aaaaacaaaa atttttagct taatatatgt ttacaaacaa gtttatatgt tataataatt
      841 ccaaaatgca actaaaatat ataaaatgtc cggatataca acagcgaaac ggaaggatga
      901 tgagtttgat aaagagcatg acgggctttg gaaggggaga attcacacag tgtaccagca
      961 ctttttccgt tgatgtaagg tcggtaaatc acagatactg tgatgtgtct gtaagaatgc
     1021 caagagcgat gtccgcattg gaggagaagg taagagaatt cgtcagcgaa aggataagca
     1081 ggggaaaggt ggatgtgtac ataaactata gtactttcgg gcagaatgca cgggtaaagc
     1141 ttgacaccaa ccttgcaaag gcatatgtgg attcacttaa tactctgaaa gagatatttg
     1201 aaataaatga tgatattaat ttatcgctgc tttcaagatt ccccgatatt atggctttgg
     1261 agactgttga gcaggatatg gaagaactct ggctgattct caaagacgca ttggaaatgg
     1321 cattcaatgc attggtggaa atgagaaagc gggagggaga aagactcaag aatgacctcc
     1381 tggaaaaagt cgcagttata aaggattatg tagaaggtat aaaggaaaaa tcatctggta
     1441 tagttgatga atataagaat aagctatatg atagaataca ggaactgata aaagatattc
     1501 ccatagacga aaacaggctc ctgactgaag ttgctgtttt cgcagacaaa gcaagtattg
     1561 atgaagagtt agtcaggctt ggaagtcata tggatcagtt taagaaagca cttaacttca
     1621 acgggcctgt cggcaagaag ctggatttca taatacagga gatgaacaga gaagtaaata
     1681 ccattggttc aaaaattacc gatttggaaa tattgaataa tgtgataagc ataaagacag
     1741 aaattgaaaa aataagagag caagtacaaa atattgaata acggagggtt aaaatgggta
     1801 tcaagcttat aaacataggt ttcggcaata ttgtatcggc aaacagatta gttgcaatag
     1861 ttagtcctga atcagctcca atcaagagga taatacagga ggcaagggac agaggcatgc
     1921 ttattgatgc tacatacggc agaaggacga gagctgtaat aataaccgac agtgaccata
     1981 taattctttc tgcagtgcag cctgaaactg ttgcacacag gcttgtggat cgcgaagcag
     2041 aagacaatga agaggttatg gccgaggaat aaagaagggt aggataaata tggggaatag
     2101 gggtctgctt attgttattg cagggccttc aggcgtggga aaaggtacca tatgcagaga
     2161 attgcttcga aattacggcg aaaaccttga actttctatt tcagttacaa ccagaaagcc
     2221 cagagatgga gaagctgaag ggattcatta ctttttcagg gacatggaag agtttttcaa
     2281 aatggttgag aatggagaac tcatagaatt tgcaaaagta catgataatt attatgggac
     2341 tcccaagaga tatgtcatgg aaaagcttga ggaaggaaag aatgtaatac tggaaataga
     2401 tgtgcagggt gccatgaatg tcaaaaagat ttttaatgaa gctgttctta tttttgtaat
     2461 tccgcctacc tttgcagatt tgaagaaaag aatagaagga cgtgggacag agacaaaaaa
     2521 agatatttgt aaaaggctga aaaacgctta tgatgaaata aaccatgccg ttaattatga
     2581 ttatattata gtaaatgatg atataaagga tgcagcagaa aagataaact gcattcttat
     2641 tgcagaaaaa agcagattgt caagatgtaa tatagatttt gagaaattca gggaggaata
     2701 agatgatata tcctacttta atgtcattgt tggaaaaggt tgatagtaaa tatacacttg
     2761 tggttgctgt tgcaaaaaga gccagacaat tggtggatgg ccagacaaag cttacaaatg
     2821 taaattcaaa taagccggta acaatagcaa taaatgaatt atatgaagaa aagattacct
     2881 atgagagaac caagccggta aaaatatcgg gggatcaaaa tgcttagtgg aaagaaaata
     2941 gttcttggtg tatcgggcgg aatagcagca tataaggcct gtgacatagt aagcagactc
     3001 aagaaactga atgcggatgt tcatgtcata atgacggctt cagccgaaaa atttgttact
     3061 cccctaacct tccagtccct ttcattaaac caggtggctg ttgatatgtt tgatacgccg
     3121 aactactggg agatagaaca tatatccctg gccaagctgg cggatgtaat tgttattgct
     3181 cccgcaaccg caaatataat tggaaaactt gcaaacggta ttgcggatga tatgctttcc
     3241 accaccatca tggccactaa ggcaaaggtt ctgattgcac cggcgatgaa taccaatatg
     3301 tacgaaaatc ctgttcttca aaggaatatc aagcttcttt cggaattggg atatattatg
     3361 attccgccga cggaggggcg ccttgcctgc ggtgatgtgg ggaagggaaa gatggctgaa
     3421 cctgctgtta ttgagaaatc aattattgat ttgctgaacc ccgggcagga tatggcaggc
     3481 agaactgtcc ttgtgactgc gggacctaca agagaagcta ttgatcctgt aaggttcata
     3541 tccaacaatt ccacaggaaa aatgggatat gcaatagcca ggcaggcagc attaagggga
     3601 gcaaaggttt atttggtctc cgggccaacg attcttgata cgccgattgg tgtagaaaga
     3661 tataatgtca tatccgctga ggagatgtac agcaaggtca tggagctttt cccgaagtgt
     3721 gatatagtaa taaaggctgc tgcagtagcc gactatgcac caatgaccgc atacagccag
     3781 aaggtgaaga agtcgggaaa ccagcttgaa ctgaagctaa agaagaatcc tgatatactt
     3841 tttgagcttg ggaaaatcaa aggcgacaaa atacttgtag gttttgccat ggagactgaa
     3901 aaccttgtag agaatgccgc agaaaaagta aagaaaaaaa atctggattt tatagcagcc
     3961 aatgatttga acgaggcagg agcgggtttc gcagcggaaa caaatgcggt aaagcttatt
     4021 gacagggaag gaaatatcga gaacattcct cttaaaatga aggatgagat tgcagatata
     4081 attcttgata agatagtaaa tttaaagcgt acatagcagt acgcttttaa gttatgtgca
     4141 gcactgaaaa taccggattg cattggggga aatcaaaatg ggaaaaatgt acgcagaggt
     4201 cgtagtgtcc aataagtgca aagaaacaga cagaacttac agctatctgg tgcctgaaaa
     4261 tatgaaggag tcgatccggg taggcagcag ggtaattgtg ccttttggca tgggtaataa
     4321 gcagatagag gggtatgtgg tgggcttgaa ggacagtata gactttaagc cctcaaagat
     4381 aaagtccata ggcaggctgt tggatgagaa acccgtaatg tcccccaagc ttgtagaatt
     4441 ggcaaaatgg atgaaggaaa aatacctgtg ttacaatatt gatgccatac aggccatagt
     4501 gccttctccc gtaaggacaa aaagcagtta cagcattgag ctttgcggct gtgaacaaaa
     4561 tttagaaaag cttgaaaaat tgaattcaaa catcattctg gaaatagtgg agtttcttga
     4621 ggaaaactcg ggaagtgcca agctggagga aataagagaa tatttcagcg acaggaaaat
     4681 agattattat atcaagaagc ttattgaagc agaggtagta aaaaaagtcc agataatcgg
     4741 aagcaaaatt ggggaaaaaa agcagaagcg tctgtacttg tcggaagata agaatataca
     4801 gataaagaag actgccagca aacagagaga ggtatatgag ctgcttctaa agtgcccggg
     4861 tatatcccct gcaaagctta aggaagcttg cggggattgt ggttcggcag tcaaggctat
     4921 ggagaaaaag ggatatgtta gaattgaaga aacggatgaa tacaggcggg tctgctccaa
     4981 aacatatgct gaaaaaagac atgaactgac ccaggatcag aataaagccc tgtcagttat
     5041 caggaactgc ttttccaacg gaaaggatgt tttactcttt ggggtaaccg gcagcggcaa
     5101 gactgaaatt tatcttgaac tgattgaaga gtatatatca aagggcaaag actctataat
     5161 gcttgtaccg gaaatttcct tgactcctca aatggtatca agattcaaga accgtttcgg
     5221 tgacaatatt gctgttatgc acagcagcct ttcagaaggg gaaaaatatg acgaatggag
     5281 aaaaatcagg gacggtattg taaaagttgc tattggtgca agatcggcag tttttgctcc
     5341 ctttaaaaat ttggggatta tcataattga tgaagagcat gaacacacat acaaatcggg
     5401 gataaagccc aagtatttaa cccgggaggt agcagagaaa agatgcaggc ttgaaggtgc
     5461 aaaccttctt ttgggttcgg ccaccccttc cattgagacc tattacagag cacagaccgg
     5521 agaattgggc atggctgaat tggggtcaag ggcaaacggc agtcctatgc ctgaggttga
     5581 ggtagtggat atgagggagg agcttgagtc cggcaacagg acaatattca gcaggagcct
     5641 gactgatgcc atattggata accttaagaa caaggaacaa atgatattat ttctgaacag
     5701 gagaggccac tccacctttg tatcctgcag acagtgcgga atagtgatga aatgtccgag
     5761 atgcagtatt tcactgacct atcatatgaa aaacgaaaag ctggtttgcc attactgcgg
     5821 atatgaaagg gataatcccg ccatatgccc gaaatgcaag agcaaaaata taaaatactt
     5881 tggtgtgggt actcagaagc tggagaagga gtttcagagg aagttttcag caaaggatgt
     5941 gctgagaatg gatgcggata caaccacgaa gaaaaattcc caccaggaga ttttggacaa
     6001 attcaaaaga aaggaagcaa atgtactgat gggaacgcag atgatctcaa agggcctgga
     6061 ctttcctgat gttacattgg ttggagtgat tgcggcagat acaagcctta atctgcctga
     6121 cttcaggtcc gcagaacgaa cttttcagat aatagcgcag gtatcgggac gctcgggaag
     6181 aggcaataaa acggggcgtg taattgtcca gacatacagc cccgaacatt acagcataga
     6241 atatgcacga tgccatgatt acaaaggctt ttataataaa gaagttatgc ttcgcaagga
     6301 actcggatac ccgccctttt ccagtattgc aaatataatg atcagcggca ctgcggagga
     6361 agaggttatt gcatatgcta atgaaataag caaatttctg gatgaagcac ttaaaggata
     6421 taaggatgcg gaaaagctgg ggcctgcagc agcggcaata tcacgaataa aaaacaggtt
     6481 caggtggcag atattggtca agagcgagag ggaggaaaca ctgagaaaag tgcttgcaga
     6541 cctttcggaa gcctgtggtg aaggcaggtc aaatgtgagt atcagtatag atttgaatcc
     6601 tcaaagtatg ttatagtaat aatagaagaa taatgcgtcg taataagttt gtgaagtact
     6661 ccgactgtag gggaagtatg ttctcctcac tttccaaagc gggggatgca agggggcgaa
     6721 gcaccctgcc gttcaaggaa ggtgctcagc tgcggagcag cgtcgaaggg aaccataggg
     6781 ttccctaggg tcggaaaagc gtcgcgagac aggcttttcc tcctggggta ttcccgttcc
     6841 gaggtcgggt ggaaagtttc gtcaaagata tcttccccgc gtcggtaatt agctttctcc
     6901 tcgctttaaa gctctgacct aatctatcaa ctagtgacta gtgactagtg actagtgact
     6961 gaagaaaatt gctgcgagga tatgtaatag gattagattt tttaggagga taatgatata
     7021 aatggcaatt agaacaatca ggaaagacgg agatgaaatc ttacgcaaga tatcaagaaa
     7081 ggtagaggtt atagatgaca ggatcctgac cctccttgac gatatgaagg atactatgaa
     7141 aaaagctgaa ggagtggggc ttgccgctcc tcaggtcgga gtattgagga gaattgtggt
     7201 tatagatgta ggcgaagggc ttattgaact gataaatccc gttattgtgt atgagagcgg
     7261 ggagcagttt gaaatggaag gctgcctcag tatacccgga ataaagggaa aagtaaaaag
     7321 gcctgaaaag gtcattgtaa gggctcagaa cagaaaaggc aagacttttg aaatgacagg
     7381 aacagaactc ttggcaattg ctttttgcca tgaaattgat cacctaaacg gtattctttt
     7441 tactgataaa gcaataataa ccgataagga agattaggct tcgaaaggga agaagaagta
     7501 tgagaatagt ttttatggga acgccggact ttgcagttcc ctgtctcaga gagctgattg
     7561 ctcataagca tgaaatagcc gctgttgtga ctcagcctga caggccgaaa ggaaggggca
     7621 acaagctggc ggaatctgcg gtaaaaatat ttgccagaga atcaggaata tcaattttcc
     7681 agccagagaa aataaaaacc ggggaatttg ttcagatact gagggaactg gcgccggaag
     7741 ccattattgt agttgccttc ggacagatac tgtctcagga aatattggat attccacctc
     7801 ttggatgcat taatgtacac gcatcccttc ttccgaaata cagaggagca gcacccatta
     7861 actggtgcat aataaacgga gacaaaacca ccggtgtcac tgccatgtat atggacaaag
     7921 ggatggacac aggtgatatc atattaaaaa aagagattga aataggagaa gatgagaatg
     7981 caggggagct gcatgacaga ctgtcggagc ttggagcttc agctttgtcc gagacgatgg
     8041 agcttctgat tgaaggtaaa atcgagagga ctccccagaa taacgaggaa gctacttatg
     8101 ccccgataat gacaaaagaa cttggcagga tagactggtc gagggatgcg aaagatatca
     8161 ggaatctgat taggggaaca tatccgtggc ccggtgcttt ttcgtcatat aacggaaaga
     8221 tattcaagat attaagaagt caggctatgg acgataatac ggacggcaaa aattacggct
     8281 gtattggcag tgtacataaa gacagcatag ttgtaaactg cggcggcgga agccttaaaa
     8341 tattggatct gcagtttgaa aatgaaaaac ctatgagtgt ggaagcatat ctaaggggac
     8401 atactattat tgcgggcaca atactgggaa aatagacaca ggataagagg ctgcgaggtt
     8461 ccggagcagt ggcggacaaa gaataacgaa taataagtta ataatattta aacaaattat
     8521 ggagggaaaa atatgtttta tggttatggt ttagatccga cttttatttt gattttaccg
     8581 gcgataatac ttgccgcata tgcgcagtcc aaggtatctt ctacctttaa taaatacctc
     8641 agagtcagga acagctatgg gcatacggga tatgaagttg ccagaaggat attggattta
     8701 aacgggcttc gggatgttcc cgtggagctt gttaggggaa ggctttccga ccattatgat
     8761 ccaagaacga gagtgctcag attgtcccag gatgtataca acagtaattc ccttgcgtct
     8821 gttggggtag ctgctcatga gtgcggccat gcaatacagc attcggaagg atatgcaccc
     8881 cttgttgtaa gaaatgcaat tgcccctgtg gcttcttttg gttcccaggc agcgtggctt
     8941 tttattatag ccggatttat ttttagctgg gtgaatctta tagacgtagg aatacttctt
     9001 ttttcagcag cggtagcatt tcaggttgta actcttccgg tggaattcaa cgcaagcagc
     9061 agggcaatag cccttttgga aggcaacgga ttggtaccct cggacgaaat agggcccgca
     9121 aaagaagttc tgaatgctgc ggcacttaca tatgtagcgg caacttttgc ggctgtcatg
     9181 cagttggtgc ggcttttggt tctgagaggc agaagggatt agaggtatat tattcgggct
     9241 acagatagga ataatactat ctgtagcttt tgcgttaata tgttatgaat agggatattg
     9301 gggaatgcaa agtaccgacg tggggaaaac atacttcccc cacagtcgga gtactttaca
     9361 aacttctgca tatataaagg agtattactt atgaaaaaaa ttgacctggc cagagatgcg
     9421 gcagtacgga tagtacacag ggtgctgaaa gaaggggcct attccaacat tgccctgaag
     9481 gaggaacttg ataaagacag gcttttaagg gttgacaaag ctcttgtcac cgaaatagta
     9541 aacggcactc tgagaaatct tacccgtatt gactggataa tatcccgttt cataaaaatg
     9601 aaaagcatag acccgtggat tgaagatata ataaggtgcg gaatatacca gatacttttt
     9661 ctggacagaa tacccgactc tgcagtatgc aacgaaagtg ccgagcttgc caggaaatat
     9721 ggacatgaag gaaccgtaag gcttgtaaac ggagtactta gaaacatcag cagaaataaa
     9781 gagaagctgg aatatccgga taaaagcgag aatgccgcct catacctgtc tgtattttat
     9841 tctcatcccg aatggatagt aaaaaagtgg ctcaaggatt tcggaatgga attcactgag
     9901 gccctgctga aggcaaataa tgaaaaacct cctttcacaa tacgctgcaa caggcttaag
     9961 attgagaaaa aggggctttt ggcaatactt gcggaagagg gcattgaata tactgacggc
    10021 agctataatg cagaagcaat tcacataagg ggaacctcct caatagaagc aaaggaatcc
    10081 ttcaggaagg gttattatca ggtgcaggat gaaagctcca tgctggtggc tcatattgtt
    10141 gatccgaagc cgggggataa agtccttgac atatgcagtg cacccggagg aaagactacc
    10201 cacatggcgg agttaatgga caataaagga gagattgtgg ccagggatat ttacaggaat
    10261 aagctcaggc ttgtagagga aaactgtgac aggctcggga taagcattgt aagaactgaa
    10321 ctctttgatg caatggtaaa ggatcagggt tccatcggga aatatgacag ggttctgctg
    10381 gacgcaccct gcagcggttt cggtgtgata agaagaaaac ccgatcttag atggaaaaag
    10441 gatgcgaaga atttaaagga attggttgat atgcagcgga ggatactgga gctggcttcc
    10501 gagtatgtaa agccgggagg aaaattgata tacagtacat gtaccataaa cagagcagaa
    10561 aaccttgatg tggtaagagg ttttctgtca aataacccgc agtttcagat ggagagtatt
    10621 ataggccaag taccggaaaa gctggagaat gacagtgccc atatgggata tcttgtgctg
    10681 tatcctaacg ttcacggtac ggatggattt tttatagcta aaatggaaaa gaggtaattg
    10741 aatttgccgg ataagaagaa tattaaagac ctgggatttg atgagcttga aatatatatt
    10801 aaagagcacc ggctgcccgg attcagggcg gcgcaggttt tcgaatggat atataaggat
    10861 acggcttcct ttaaggatat gaccaatctg cccaagaacc tgattgagca gctggaatgg
    10921 gatttccata tcggcagggc atatattgcc gcaatgcagc actcaaaaac agacaatacc
    10981 agaaagtacc tgatttgctt tgaagacggt aatgccgtag agtgtgtgct catggactac
    11041 agctacggga aaaccctatg catatcaaca caatttggat gcagaatgtc ctgcgagttc
    11101 tgtgcttcaa caacgggggg gcttatcagg tccatgacca gcggagaaat gcttgaggag
    11161 gtaatggctg tatcccggga tatcggagaa agaataggca atattgtact tatgggtacg
    11221 ggggaaccct ttgacaacta tgatcaggtt atgaagttca taaggacaat taatcacccc
    11281 aaggggctga atataggcca aaggcatata accctttcaa cctccggaat tgttccgaga
    11341 atatacgatt ttgccgatga aaagcttcag tgcacactgg cgatatcact gcacgcagct
    11401 gatgatgagg ccaggagcag aataatgccc ataaacagga aatataacat tgagaagctt
    11461 atggaagcgt gcagatacta tataaatatg acaaataagc ggattacctt tgaatatgca
    11521 ttgataaaag atgtcaacga cagtgttaag gatgcccata agttgggaac actgctaaaa
    11581 ggaatgctct gccatgtgaa tcttattccg gtgaataaag tggaggggaa aaatttttca
    11641 aaagcctcca aggagaggat aaattccttt aaggccgctc ttgagggctt cggaattgaa
    11701 acaaccgtca gaagggaatt gggcagtgaa attgacgcag cctgcgggca gctgaggcag
    11761 aagcacataa atcggcaggg agtcacaaat atttgaattt gttagaggtt ttttatatgt
    11821 tttgtcgaaa tatttttttt gtaatgaaaa ccgaaggcaa ttttaattcg gctaagagta
    11881 tatttgaggt gatttaatga ggtcagccgg caggacggac ataggtctgg tcagaaaagt
    11941 aaatgaggac agatttttat gcgaaaaact tgaggacatc gacagcacgt atttatacat
    12001 tgttgccgac ggcatgggag gacataatgc aggagaggta gcaagttcaa tggcggtaca
    12061 ggaggtagct tcttacataa aaaatagtat cggaaccctt atggcgggag atgaagaaat
    12121 taaggatctt gtcagaaatg caattatgta tgccaacgaa aagatttaca agacgtctat
    12181 acttaagagc aactgccttg gtatgggaac aaccctttca atggtgcttg caaaggacag
    12241 cagcatatac ataggacatg taggcgacag caggatatac cttatcaggg ggaaagacat
    12301 atcaaggctt acggaggatc attccctggt tgcggagttg gtaaagagag gtaccataaa
    12361 accggaggat gcatctaatc atccgcagaa aaacgtcatt acaagagccc ttggaacgga
    12421 atactcaatt gaaccggatg tataccaata tgatatgcag agcggcgatc ttatactaat
    12481 ttgcactgat gggctgtcaa atgcggtgtg tgaagaggat atagccggtg taatgaatgc
    12541 tgcttctgat ttaaacgggg cttgcgagct tctgatagaa aaagccaagg aaaagggcgg
    12601 ctatgacaat ataacagccg tagctattca ggtttgtaag ggtggtgaag ataatgatag
    12661 gtagagtatt gggagataga tatgaaatat tggaaaaagt tggcggcggc ggaatggcct
    12721 tggtgtataa agcaaaatgc agactgttga atagatatgt tgctataaag gttttgaggt
    12781 cggaatttac cgaagatgag gaatttgtca agaagttcag gagagaagcg caatcggcag
    12841 ccagtctttc acatccgaat attgtcggga tttatgacgt aggaaccgaa aacaacagct
    12901 attatatagt tatggagtac atcaaggggc agactcttaa agagcttata aagagcaaag
    12961 ggaccttggg agtggaatat tccaccaata tagcaattca gatatgttat gcattggaac
    13021 aagcccataa aaacaatata gttcaccggg atataaaatc tcacaatata ctgatacggg
    13081 aagacaactc tgtgaaagtc acggattttg gaatagccag ggccgtgtct tcaagcacta
    13141 taacaaatac aggcaacgta atcggctctg tgcattattt ctcacccgaa caggcaaggg
    13201 gaggatatgt cgatgagaaa tcggatattt attcactggg agtcgtaatg tatgaaatgc
    13261 ttacgggaag gcttcctttt gaaggtgatt ctcctattgc tgttgctttg aagcatatac
    13321 aggaagagcc ggagcctccc tccgggatta actccaggat accgaaggcc attgaagcaa
    13381 taataatgaa gtgtatggaa aaggaagtat ccaaaaggta taacagcgct tcggagataa
    13441 taaatgacct cagacagtca ttggtaatgc ctaacggaga ctttgtaaag aaaaacaggt
    13501 ataccgatga aagtaccagg gtccttgagc ctataaaaat aattgacaag gaagcagcgg
    13561 ttgatattat tgaagaaccg gaaagcaagg aactgaatga aggaaaagaa gaaaaaatcc
    13621 tcattgatga ttattcggtt gatccaatcc caaaggtagc cgagggaaag gataaacccg
    13681 tacagaataa aaggaacgta aagcttacta ttgtcgctgt ttttggggga ttactgctgg
    13741 ctttgctgct gggcggagga atattgttcg ttaacagcct ctttaccgta aaggacgtgg
    13801 aggttcctga catcaggaac aatagcgaag aagaggcaag ggaaaagcta agtgctctca
    13861 gccttatact ggaagttacc gaaagggtat ttaacaagga tattcccgaa ggaagcatta
    13921 tcagccaaaa tcccaagccc aacgagaaga acaagataac caatcccgta aaggttgttg
    13981 taagcaaagg gcccaggaag gttgtagtcc cgaagcttga aggcgaaagc tatgacaagg
    14041 tcaagctcat acttgaagga gaaggactgg ttgagggttc ctttgaccag gaatacagcg
    14101 aatatcccaa tggttttgta ataaggcaga gcatagctcc gggtatcagt gttgatgaag
    14161 ggactacaat agactatgta ataagtatag ggccggaaaa attcataatg ccctcatatc
    14221 ttggagcaga tattgaagat gttaagacgg atctgattat caaagacctt atattgggaa
    14281 acatatccta tgacagcaac agtgagcagc ccgcagacac agtgctggag cagagcataa
    14341 ggccgggcac tgaggtcagc aggaaaagcg tagtggattt tgtcgtcaac aactataagc
    14401 cggagcctgt accctccagc ttcaagcttc gtttcacact gccgccggag cttgagagta
    14461 tgaaggtgac ggtatataaa gtacagaatg aaataagcag cagaatatat gaaagtattc
    14521 acaaatcaac cgacagcccc ctggaaatac cggtaagcgg tgtagggaat gtaatttttg
    14581 aagtatatat aaatgacagc ttgcacgaaa gcgtagcaca caacttttaa cgaggaggga
    14641 atgtatgcag aaaggtgtta tagtaaaagg catcggcgga ttctactatg ttgacacagg
    14701 ggaaggggta tacgagtgcc gtgccagagg caaattcagg ttaaagaaca taacaccttt
    14761 aataggggac catgttgaaa ttgatgttga tgcgggaagc ctgcagggtt atattatcag
    14821 cattctggag aggaaaaacc agctaataag gccgactgta gccaacattg accaggctgt
    14881 gatagtattt gcggcaaaaa agcctgatat aaacatggtg cttatgcaga agttcctggt
    14941 ttattcggag tataagaatc tgaatgtcat agtttgtata aacaaaatag atttggaccc
    15001 ggatggccac tatcttgatg tggtcagaat gcttgagggt attccctata aggtaataac
    15061 caccagcgcg aaaacaaaag tcgggataga gcagctgcag gaaatactta aagataaggt
    15121 ttcggttttc gcaggcccct ccggtgcggg aaagtcatcg ctgctgaact gcattcagcc
    15181 ggggctgaag ctaaagatcg gagacctgag caaaaaaata gacagaggca cgcataccac
    15241 gaggcatgca gagcttataa agcttgatga gggcggcatg gtagcggaca ctccggggtt
    15301 tacctctctt gatattggcg aactggagct gtcgaagctt gaaagctgct ttccggaatt
    15361 tgaaagccac aagaattgcc aattcgttaa ttgcatccat gataaggaac cccgctgcgg
    15421 gataagagag gcagtactcg agggcaagat acacaatata agatatgatt tctacatatc
    15481 tctgttaaat gaattgaaga gtactggggg gaggaattat aaatgattaa gattgcgccg
    15541 tctatattat ctgcggattt tgcgaatctg gagagggata taggtacagt ggagagggca
    15601 ggtgctgatt ggcttcacgt tgatgttatg gacggacatt ttgttccgaa tataaccata
    15661 ggcccggctg ttgtaaaaag cttgagggca aaaagcagcc tggtttttga tgtgcatctt
    15721 atgattgaca atcctgagtt atacataaat gattttgcgg aagccggtgc cgatatcatt
    15781 acagtacatg cagaggcttc ggtacatttg cacaggttgc tgcaaatgat aaaggcaaag
    15841 ggcctgaaag cgggtatggc attgaatcct gccactcctg tcaatgtact ggagcatatt
    15901 atagaggatt tggatatggt attgattatg agtgtaaacc ccggctttgg aggacaaaga
    15961 tttattaatt ccgcttatga aaagatatca aaggtaagaa gcctcattga gaaaaagaat
    16021 ctgtccgttg atttgcaggt tgacggagga atagggcctg acaatatccg gcaggttgtc
    16081 atgagcgggg ctaatataat tgttgcaggt tcggcaattt ttaactcggg aaacataccg
    16141 gaaacaataa gaattatgaa ggaagcaggc ataagtaaat aatgaaaact gtaataatca
    16201 gcggcggtga aataaaggat tatgaattct gcagcggtat tgtcaggtct gcagacaggg
    16261 ttatctgtgc ggacagcggt acaaggcacg caatgaatat gaatctgact cctgatgtaa
    16321 taataggaga catggattca tcaccggccc agtatataga ttttttcagg aaaaaaggcg
    16381 ttgaagtcat acagtacccc aaggagaagg ataagaccga tacacacata tgtgtggaat
    16441 acgcactgga cttctcgacg gaaataatat tgctgggagc gacagggagc cggattgacc
    16501 atatgattgc aaatatctct ctgttgatgc ttggaatccg gcggaacata cccatgagca
    16561 taatagacaa caagaattgt atcaggatga ttggggattc cattacactg aaggggaata
    16621 aaggtgagtt tttctccctt ataccctata cagggaaagt agaaggactg agtaccaggg
    16681 gagcccatta cgagcttgag aatgcagtta tggagctggg gaacccgtat ggcgtcagca
    16741 attattttga ggaagaaacc gtaagtataa ccattaagaa aggctttctg ctggtaatta
    16801 agtcggagga ttagaaaccc gagagaaaaa aatcagcttg tcggcttgaa aaatccgtgt
    16861 caaatatgac tctgcattag tattatataa tgtagggttt ttatcttttt ttgccattat
    16921 tccgtcaggg ggattcaaca atgaggtata catataagag atatgccggc tgcttgtgta
    16981 tcatactgtc tgtctttctt gtttcaagat tcatgccgct ttttctatgg tgttttgcag
    17041 tggcggcagc tgtcgcaatg ataggatttc tgctgtacaa ttgctttttt atgtagttag
    17101 gaggaatgac atcatgagtt tattaaaacg aaaaaaactg actggcttcc ttgcattctg
    17161 catagggctg ggaatactct ttgccgtcct ggttcctgca ataggctggg taatgttctc
    17221 ggcaatctgc cttattagca tcggaatatt ccttataaaa tattgagaga tgttatttca
    17281 aaaaagacaa aaagcgctga atcgcgcttt tattttcgat tttagccttg ttatattgcc
    17341 ctctgtactt tacccgatct taaacatctg gtgcatacat gcaatctctg tggagtaccg
    17401 tttactacgg cttttacagt tctgacgtta ggcagccaga tcctgcggct atgtctgttt
    17461 gagtggctta ccttatttcc ggaaatcatg ccttttccgc aaacctcgca atgtctcgcc
    17521 atggaataca cctccttata aaaacaaata tattttaaca caacccgtta taaatggcaa
    17581 caatatgttt ttaagggctg caaggctgaa gaatggcggt gttgacgcaa tcctataatg
    17641 tactttactt atacgggcaa attatagtat catttattat gtcacaattt gatagtatta
    17701 tttgtatcat ataacatact tattatgagg cgaaagtaag aaaatttttc taaattcgaa
    17761 aaatttactg ctgatgcgtt ataatatata ataataataa aaagcgggag gagatcacat
    17821 gtctgccata tacaaaactc agtacggtga agttaatata acagatgatg ttttggcaac
    17881 catttcagga cttgcagcta ctgaatgcta cggtttggta ggaatggcaa gcaagaatgc
    17941 taaagacggc ctggttgagc ttcttaagag agaaaacctt gctaaaggtg taaaggttac
    18001 aacagaaggt gaagagataa tagtggattt atatattgtt gtcgaatttg gtacaaagat
    18061 atcaactatt gcagataata ttatcagtaa ggtaaaatat actgtagaaa acctgacagg
    18121 cttgaaagta aaaaaagtaa atataaatgt acagggcgtc agagtataac tctggaaagg
    18181 aggtacatat cttgtcaaca aaaaatattg atggtataca gttaagaaac atgtttttat
    18241 ccggcgcaaa taatctggaa aacaataaag gaatcgtaaa ctctctgaat gtatttcctg
    18301 tacctgacgg cgacaccgga acaaatatgt cgcttacaat gcatctggct gtgaaggaaa
    18361 tcatagctgt caagagtatt gaaatacaaa gcgtggccaa tgccgctgcc aatggttccc
    18421 ttatgggagc aaggggcaat tcgggagtta tactttcaca aatatggaga ggctttgcca
    18481 aggcattgaa gaatcaaaaa agtatggatg taaatttatt ggccaatgca cttacggaag
    18541 gctccaatac tgcctacaaa gcaataatga agcctactga agggactatt cttacagtca
    18601 ttcgcgaaac agcagagtat gcaatgaagg tttcagagaa ctttgaggat aatgtactat
    18661 tttgggaaga aataatagag aaggcaaaca gtgtattgcg caggacccct gaaatgcttc
    18721 aggtgcttaa gaacgccgga gttgttgatg caggcggaaa aggacttata tatatatttg
    18781 aaggaatgta tcagtacctg aagacgggtg aaatgataga actgaaagct gcagcagatg
    18841 atgaagagca tgacaggccg ctgtttaaag aagaggaaat tgtgttcggc tattgtaccg
    18901 aattttttat aaaaggaaga aaactcgggc tggaaaagtt cagagacgaa atattgccga
    18961 ttggagactc acttcttgtt gttggggatg agaatctttt gaaggttcat atacatacca
    19021 acaaccctgg cagggtgctg gaactggcga tggcccaagg tgagctttcc aggataaaga
    19081 tagataatat gagggaacag cacaatgaac tgctgtttaa taaggaggag cacaaagctg
    19141 cggaagaagc ggaacaagct ccaaaaccta tggagaggta tggaaccata gctgttgcaa
    19201 tgggagaagg catatcttcc atattcaagg acttgggggc aaacgaaata atagaaggcg
    19261 ggcagacaat gaatccaagt actgaggata tattaaacag tgtgaataaa atcaatgctg
    19321 aaacgatata tatattccca aataacagca atatcattct tgccgcaaat caggcaaagt
    19381 caatgtctga taaaaatgtt atagttgcgg cgacaaaatc catccctcag gggatatccg
    19441 cacttatggc gcttaatcat gataaggaaa ttgaggaaaa caataaaaag attgaaaagg
    19501 caattacaaa cgtaaagtca ggtttggtta cctatgcagt gagaaattcc aactatgacg
    19561 gaataaatat agaagagggt aatgtacttg gcatggttga agggaaaatc agcggggtag
    19621 gcaatgatat tcttgaagtc agccataagg ttcttgatga tatggtagat gaagactctt
    19681 ctttgatatc catctactat ggcagcgaag tgaatgaaga tgaagccctg aagctgaagg
    19741 aagaaatcgg cgaactgtac aataactgcg aaatagaact gcacttcggc ggacagcctc
    19801 tgtattatta tatactctca gtagaataaa ttattgaggt gtacaaatga attatactga
    19861 agattccata accaatttaa agggaatagg accggtaaag gccaggcttt ttaataagct
    19921 tggcctgtac actatcggtg atttgctgga gtactttccg aggggttata aagataaaag
    19981 cgagattaca aaaatcaggg atataatgcc cgatgaaagc tgtaccgtaa gggtgcgtgc
    20041 cgcaggaaag gcccaggagg gcagaaccag gagcaggctt attattacaa agctgacggt
    20101 atttgatgat acaggcaggt tgaatgcagt gtggttcaac aacagattca ttaaaaatgc
    20161 tttcagggaa ggggacgaag tactgatgca cggcaaggta aagcgcatcg ggaaggatct
    20221 ggtcatggag gtccccgagt atgagaggct gtcagacagt gaaagcataa acctgatgag
    20281 aataacgccg tattattccc ttactgaagg cctgtcacaa aaggatataa gaaaggcggt
    20341 atacgcagca ttaaaacatg ctgaaaagca ggcggatttg tttccggaag aactgcggag
    20401 aaaatacgga cttgccgaga ttaatttctg catcagcaat attcattatc cggagtctat
    20461 ggataagctg aagctggcaa ggaaaaggct gatatttgaa gagtttttta tgctgcagtc
    20521 cgggttgata catataaaaa agctgaacag cgccgggcaa aagggtatca ggtttaaaaa
    20581 gacaagtgaa ataaaagagt ttattgaaca tttgcctttc aagcttactt cagcacagga
    20641 aagggttttt gaggaagtca gcaaagacat ggaaggttcg ggcattatga acaggcttgt
    20701 acagggggat gtgggttcag gcaagacggt cattgccgct cttgcactgt tcaaatgtgt
    20761 aagaagcgga tatcagggca caatgatagt acctacggaa atactcgcag aacagcatat
    20821 ggagacttta acaaacctgc tcaaaaacac aggtgttgaa attgcactgc tgaagggcgg
    20881 aatgacaggg aaggaaaaac aaaaagtgct ggagggaata aaagaaggaa aaatagacgt
    20941 ggttgtggga acccatgcaa tcattcaaga cagtgtaagc tttgataagc ttggacttgt
    21001 tattaccgat gaacagcaca ggttcggcgt aagacagaga gcattgcttt cggcaaaagg
    21061 ggaaaatccg gatatgctgg ttatgactgc aactcccata ccaagaacac tttcacttat
    21121 agtatacggg gatcttgatg tgtcaataat agatgaaatg cctcccggtc gaaaaaaagt
    21181 agagacttat ttcatagatt ccgccaaaaa ggaaaggctt atgaattttg tcaaaaaggg
    21241 ccttgatgaa gggcggcagg catattttgt atgcccgctt gttgaagagt ccgacaagct
    21301 ggagcttaaa tcagcgatag agtacagtga ggaactaaaa aataaatact tccggaacta
    21361 taatgtggga ttgcttcacg gaagaatgaa agccgatgag aagaatgaag taatgtacag
    21421 gttcagggaa aaagaaattc atatacttgt atcaacaact gtaattgaag taggagtaaa
    21481 tatccccaac gccgcctaca tggtaataga agacgctgac agatacggaa tggcccagtt
    21541 gcaccagttg aggggcagag tcggaagagg cggacaccaa tcctattgca tattaatttc
    21601 tgatacaggg aatgaaatag cgcaggaaag gcttaagtat atgacaaaaa cggaaaatgg
    21661 ctttgaggta gccgagaagg acatggagtt ccgcggaagc ggggaggtac ttgggcaaag
    21721 acagcacggg cttcccggct tcaagcttgc tgatgttttt cgcgatgcga agcttcttaa
    21781 gctgtcaaag gaagcagtag aatacatata tgaaaataat aagctttcca atgaggatta
    21841 caagaagatg aaagaaatac tggatcaaag gttccagaag atgctggatg aaataacgct
    21901 taattaggcc tcgaatattt tcctaagtta acataattta cagtgtttaa aaaactgtaa
    21961 acggagtaaa ttaataatac ataaaccgca ccaagcattt acgctgcatc ggggctgcat
    22021 tcgacgggac tattgcggtt tatacacgta atacaaacag gaggtgctac atatgccaag
    22081 aaataccaca tcaaacagga ccttgatacc ggaagcaaag gctgctttga agactttcag
    22141 ggatcagata gctactgaat taggaacgga tcttacaggc tataatggtg ataagacatc
    22201 ctgggagtgc ggaaaagtag gcggagaaat ggtaagaaga atgattgaac aggcagaaag
    22261 aagcatgtca ggaagaagat aaaagataaa ggtaaaagaa aaagaaaggc gctggctgca
    22321 taggtcagcg tcattttttt agcgaaaaaa tactgatgac aacactctaa ttcaatgtta
    22381 aagcattgac atcagtatcg ggagaggaga aattgaagga agagctctat ggggaaattt
    22441 agttacaaat aactaacgtc cctgatatat atgttgttca aataagtttt ttttattcaa
    22501 ttaaaattat tttacatacc ttcctttaaa tacatttata aaaggccccc atttttatca
    22561 gactgttaat gacagctttc tcatatcgca gaatctgtcg attccctgac atttatgata
    22621 taatatgcgc aggtgcatat tatattcctt atataattgc cgcgcgatat taataaattt
    22681 ttgtataaaa atagtattta tgttaaaatg taattgaaat gggcaaaatc aatcggtgtt
    22741 attgacaata aaagacttgg aggtaatctt ttgcgggtta ttgcaggaga gtataaggga
    22801 agacggcttg atcccattga gggcagggac atccgcccta cctctgatat ggttaaggaa
    22861 tctcttttta atatacttgg ggatgctgta attgacagcg tttttctgga tttgttcagc
    22921 ggtaccggag gaataggcat tgaagcattg agccgcgggg caaagcatgt ggtattcatt
    22981 gattccggca tcaaaagcat aaaggtactg aggggaaacc tgaaccgtct gaatatcaag
    23041 gattgtgctg aagtatataa tacggactac gggacggcaa tcaacaaatt gcacgaacat
    23101 ggaaagagtt ttgatttaat ttttattgat cctccttaca gtatgggaat ggctgaatca
    23161 gccttgaagg aaattgataa taaaaatatt cttacacaat ccggtttgat tgtagttgag
    23221 catgattcaa aggaggatat gccgcaaaaa gtggggaagc tattcttata ccgtttcaag
    23281 aaatatggaa atacaatact atcattttat tctattgacg aacagtcgca gcaaaaggag
    23341 gagtaatttt gaaaatagca gtgtatcccg gaagctttga tcctgctact aacggacata
    23401 ttgatattat taaaagatcc ggagcacttt ttgacagggt tattgtagca gtattgagca
    23461 atccgagtaa gaatccgatt ttttctgttg aggaacgagt ggacatacta aaaaggacat
    23521 gcggagatat aaagaatgtt gaaattgaca gtttctcggg gcttttgatt gattattgca
    23581 agagtaaaaa cgcaaatgta atagttaaag ggttaagggc agtttcagac tttgaatatg
    23641 agctgcaaat ggctcatatg aataagaagc tcaatcagga aatagaaacg gtgttcataa
    23701 tgactagcag ccgatattca tatctgagtt ccagcctggt aaaggaagtt gcaggatttg
    23761 gaggctgcat aagagggctg gtaccggaaa ttattgagaa ggagatttat gaaaggctta
    23821 aatagcggtt caggcacgga ctatcatacg tgtgaaataa atattcacaa atgaattatt
    23881 acagggaggt tggtattatg gaggttatgc tgctgctcga cgcccttgaa gatatactgg
    23941 ataaggcgcc gaatctgccc ttatcaagta agtccatgat taataaggaa gaattgcagg
    24001 aaataataaa ggaaataaga ataaagctgc ccgatgagat aaaacaggct caatggatta
    24061 aggaagaaag gcagaaaata cttatagaag caaaaaaaga agctgaaaat ctgagaaaag
    24121 aatgcgaaga gaaactgaac aaggttaagg aagaacgtca gaggattctt gccgaagctg
    24181 aaaaagaagc tgaaatactt aagaaggaag ctgacaaaaa ggttcaggag cttattgatg
    24241 aaagtgatat aataaaaaag gcaaatgaac aggcaaaaga acttattatt gctgctcaga
    24301 tagatgccaa aaagataagg ctgggttcca aagcatacgc ggacgagctg ctggcggaac
    24361 ttgaaaaaca cacggaaaaa ataatagaaa caataaggaa caacagggat gagcttaaga
    24421 attacaagag ctgataagtg gtacaagggg gacgtttctc ttgtaccctt ttaatttatt
    24481 catttgggac aggagaaacg tccccatgta cctttaatat gcaccgcgga cataaaaagc
    24541 aatattatca gcatatttgc ggcagaggta accaataccc gcaaaaagct gtattccagg
    24601 atcgtatttt gcacaaatga ttctgccgga aggttaaaaa cctgaagatc agaaaaccag
    24661 gcaaccagta cataggagta gaatgcagcc gcaattccct gaaaaagctt ggtaatgaaa
    24721 taaataccga gcttaagatc cgttccggat attatactca tcacctgggc atttactgaa
    24781 agccctccaa atcctatcat gaaactcgtt atggcagcct tgtatattaa gggcacccgg
    24841 agagaggcac atgacttcgc cccgttggtt acttccagta agccgataag cagactggat
    24901 ataatatcgg gagttatgct cttaaggggg attgcccttg aaaggctgag ggagaaccat
    24961 tcgagaaagc ctgatattct cagcatgccg gttatcacag aaaagaagat aataaaaccg
    25021 cctatcataa gtatcaggtc gattccgttc tttaccgcat ctctcatcag cattccgaaa
    25081 gtacgtccgt ctctccttct gtactccatc atttcagcaa taggatttga tatatatttt
    25141 tccctgatgc ggggattttc tttcccataa aaacgcattg caaacccgat tgtgatggct
    25201 gacaggtagt gggcaattgc aataaaggag cctaaggccg gattgttcaa tattccggtg
    25261 gcaaccgcac ctattatgaa caaaggcccc gaggtgctgc acaagctcag cagcctctgc
    25321 ccttcagcaa gagtacacag gtttttgctg tacagttctg cggtaatttt tgcgcctaca
    25381 ggatacccgg aggatatact cataaaaaaa tcaaaggagc cttccccggg cacattgaat
    25441 accggaccca taaggggacg gaaggcggaa ccgaacagtc tgacaatacc caggccaatt
    25501 aagatttcaa cacagataaa gaagggcaga agtgcggggc agaccacatt gaaccatagc
    25561 ttcaaaccat caagtgaagc tgcaaaagcc tctcccggat atctgattat gaatgtcaca
    25621 aggatacaga agataaagga ataagcaaag gtggaaatag tgcctttttt cataaggaac
    25681 ctctaagcat aagtatttta attaatatat ttatatttag agggatatga tattatgaat
    25741 gaacagagag tattactccc tgtgataata gggagaggta aaataatcct ccgcagcttt
    25801 cctgaattca gatgtcctgt atgccaggtt gtaaatgtct gtagcctgta tatcatatga
    25861 aaacattttt attattcttt tatcggtgca tctgaaatcc gccacctttg aaatcacagg
    25921 gtaagggcag cagtccatta tcattttgat aagttttttt ccattattgt tgaaagccag
    25981 tactctaaca taagacgggc attcccggct tttgaacatg gatatgtctg cattcttgat
    26041 atcaaggagc acgtgacaca gcaggcgctg gatgtaggtt cttgtgtaac gttttgtttt
    26101 gacagcttca atcagatctt cgcaattggt gctggctgct gcatatttct taagcctgtt
    26161 ttctattccc tccttaacat tagggtattt caaaagctcg gatttttcca ttgtcctaag
    26221 cttgtacagc agtatatccg aaaaatcatt catataaaca ggatgcctgt tcatcctcag
    26281 atttacactt ataatatcta tggagcttcc gggcagggac attcgaacct tgtcggagat
    26341 tccgccttct tttatttcat ttcttattgc agtggcactt gcgatgcttc ctgcaattgt
    26401 attatcatta taaccgctta cgatacgttt tacggcaaca ggtgtaatag agctattcaa
    26461 agacttgaga gctttaatat attcaatacc cagtacatta ttgggattct tcaaaatatc
    26521 atcggaagca taatgaatat catcggatgc ataatgggac aatgcgttgg aaacagcttt
    26581 gggataagaa acaccccggc cgagctgccc ttttaccaga tgtttaaagg cttccggctc
    26641 attttgaagc aaattggcta accggtgcag tgcgccggta tcccccagtt cgcttccaaa
    26701 acagatatgg tcaacaaccc ccagggaatc taatatcttt accgctccgg ccgcaaatat
    26761 ttccgccggc tggcaggcat acacaacagg aagctcgatt accaggtcag ctccgttcat
    26821 aactgccgat tttgaccggg accatttatc gaatattgac ggttcgcccc tttgcacaaa
    26881 gcttccgctc ataatgcaga cggcataatc tgcaccgcag gcttcttttg cggctcggat
    26941 atggtatttg tgaccgttat gaaaaggatt atactcggat actattccta cggttttcat
    27001 cattattacc tcccaaacct aaccttcgga acctcaaatt cattttacca tattatccgc
    27061 aggcaaatga atatttatag taggtattga aaaaatctca ttatggggat aataatcttt
    27121 gttaatattt ttacaaaaaa gcagggaaaa cgacgattag tatagaaatt cttacatgca
    27181 caatatataa tgggaggtac gtaaacgaat gaaaattttg gttattaact gcggcagctc
    27241 gtcattgaag tatcaattca tagatatgga gaatgaagaa gttatcgcca aaggtcttgc
    27301 ggaaagaatc ggaattgacg gctcagtact taatcatcag ccaaagggct tcgacaaaat
    27361 aagaatagaa aggcccatgc cgacgcacaa agaggcattg cagacagtgg ttgatgcatt
    27421 gagagacaaa gaatacggcg taataaagga tatgggagag ataactgcag ttggacatag
    27481 agtcgtgcac gcaggtgaga agtttgcata ttcagtgctt cttaaagaag aagttatgga
    27541 tgcactgaag gaatgcataa gtttagctcc tttgcataac ccgcctaata taatgggtat
    27601 agaggcatgc aagcagatta tgccggaagt gcctatggtt ggagtgtttg atacggcctt
    27661 ccatcagact atgccaaaaa agtcatacat ttacccaatt ccctatgaat attatgaaaa
    27721 gtacaggata agaagatacg gcttccacgg cacatcccac aaatatgtgg ctgagagggc
    27781 agcggcaatg cttggaaagc ctgttgaaga gcttaagatt attacatgtc atttaggaaa
    27841 cggtgcaagc attacagcag tgaagaacgg aatatcggtg gataccagca tgggctttac
    27901 acctcttgaa gggcttgcaa tgggaacaag atcaggcaac atagatgctg caataattaa
    27961 attccttatg gaaaaagaaa atatcagcgt tgccaaggct gatgaaatac taaataaaaa
    28021 atccggcgtt ttgggcatat cgggagtcag cagtgatttc agggatattg aagaagccgc
    28081 caataaaggc aatgaaagag cgcagcttgc ccttgacgta tatgttcatt atgtaaagaa
    28141 atatataggc gcatatgcgg ccgtaatgaa cggtgttgat gcaatcgtat tcacagcggg
    28201 cctcggggaa aactccgcat caatgagaag cagtatatgc aaagacatgt cattccttgg
    28261 cgttgagctg gatgaggcga aaaacaatgt cagaggaaaa gaaactgatg tgtccaagga
    28321 tggctcaaag gtgagagttc tgcttattcc taccaatgaa gaactcatga ttgcaaggga
    28381 tacagcggag cttgcaggga aggcaaaaaa ataaaaaata atgcggcgcg agccgcttat
    28441 ttttttagaa tgcattgtaa atactaacac aagcggtgtg aagtgttttt gcttgacaaa
    28501 atatatattg atttgtataa taaaatttgt taatcagaga gaaaataaca ggtggttgaa
    28561 tggaacttaa ttttgcaggg atatctaaat ccgaaggtgt aaaagagaga ttcgaaataa
    28621 tagaatatcc tattgacagt actattgtgt tctgcggtga gaatttgaat atagtttctc
    28681 ccattacggt gaaaggcagt gcggtcaact atgaaggaaa gataagtgtt gatcttacga
    28741 ttaccgcaac agtggagagg acatgttcca gatgtttgga aatcttcact gaagaagttc
    28801 aggcagattc aaattatgtt tttgtcaaag atatagcgga tgacaaggaa gactattata
    28861 tatacagagg agacaaggcg gacataacag accttgttat tggagatata gcagccaagc
    28921 ttgccatgaa gccgctttgc agggccaact gcaaaggact gtgtactgta tgcggaaaga
    28981 ataagaacag tatcgactgc caatgcaaaa gcgaagaagt tgatccaagg atgcaagctt
    29041 taagcaagct gctggacaga aagtaaggag gtgtttatat tgggtttacc gaagagaaga
    29101 cactcacaat cgagaagaga taaaaggaga gctaattgga aattggcttt gccgggactg
    29161 gttgcatgtc cgcagtgcca tgagccgaag cttcctcaca gggtatgcaa atcttgcggt
    29221 tattataaga acagaacggt tataaagaca gaagaataat aaaaaattca tgataagaca
    29281 agggaagtcc cctgtcttat ttattttttg cagacacggg gaggagacac gggggaggac
    29341 acggggaggg agacacgggg acgtttcgcg gagacacggg gacgtttccc ctgtcccact
    29401 ggaagtggga cacgcgaaac gtccccgtgt ctccctccgt gtctccctcc tccgtgtctc
    29461 cccgtatttt ttaaaaaaaa actgttgcat atttaatgtc aagtgttata tattagtatt
    29521 agaaccaggt actaaattat agtagtatat ctgagtattg gaggtttgaa tatgaagcag
    29581 gggggcatta gcaagaaaga aagacaggcg agtttgctgg acagaatcaa gacagaccct
    29641 tttcttactg atgaagaact tgcggaaagc ttcaatgtga gcatacagac tataaggctt
    29701 gacaggcttg agctgggtgt accggagttg agagaaagaa taaaaagtgt tgcagaggat
    29761 aactatacaa aggtcagatc tcttcatggc aaggaaatat tcggagagct ggttgatttg
    29821 aatcttggag taagcggaat atctatactt gaaactgata ataaccttgc atttgaaaag
    29881 acaaatgtag tgaaagggca gtacatattt gcacaggccg aatctcttgc cattgcggta
    29941 attgatgcaa atgtcgctct tataggagtt gctaatatca agtacaagaa tcccgtattt
    30001 gcgggtgata agctgatagc aaaggcagaa gtcgtaagaa ccagaggcaa caagtatttt
    30061 gtgtgggttt ttataagagt aaagcaggct gaagtattca gaggaaaatt catccttgtg
    30121 tccttcagca attagactaa gaagcggcta tatatgtcgt gaaaatgatt tatcattaca
    30181 aaaccagtta aagtaatttt cacgacatat gaattaagga aaatgtttca cactatttga
    30241 attcaataca aaatgtttag tgcgaaacat ataggaggtt tgaatatgag gatagctgtt
    30301 gatgtaatgg gaggcgacaa tgctcctgga gcaattatcg aaggatctat tctggcactg
    30361 aaagaatacg gaacagagct ttatttaatc ggtttaaggg acgagatatt gaaatacctt
    30421 ggagaggaat acaaaaatca ccaaaaggtt aatatcatcg aagcctcaga aataatcaac
    30481 gcagatgaag cacctgtaag tgccattaag cagaaaaagg attcttccat ggtaattggc
    30541 cttaagcttg tgaaggaagg acagtgcgat gcttttgtat ctgcaggaag taccggagca
    30601 tttcttgcag gctccctcct taaggtggga aggataaaag gaatagacag gccggcactt
    30661 tcaccgctgc tgccgacatt aaaggggagc tgcatgatta ttgatgcggg ggcaaatgcg
    30721 gacagtaagc cgaaaaatct tcagcaatat gctgtaatgg gatccatata catggaaaag
    30781 gtaatgggga ttaaaaaacc aaaagtcgga cttttgaata ttggtgccga agcatccaaa
    30841 gggaatgaac tgaccaagca gagctatact ctgataaaaa atacaggggt aaatttcatc
    30901 gggaatactg aagccaggga tattatcagc ggagtctgcg atgtatgcgt atgcgacggt
    30961 ttcgccggca atgtgctttt gaagaccaca gaaggtgtag ctatgacatt atttaagctt
    31021 cttaagaatg agtttatgaa aagcttgaaa acaaagcttg gggctatgct gcttatggac
    31081 agcttgaaaa atataaagaa aagctttgat tatactgaag taggaggagc accatttctg
    31141 ggtattaacg gcattatgat aaaagcccat ggcagttcgg atgcaaaagc aataaaaaat
    31201 gcaataagac aggcaaaaat gctatatgat aacaaatgcc tggataccat aatggaagaa
    31261 attatcaagt tgggagttga agacactgat gaacaagctt agaaatgcag gaataatagg
    31321 aacaggaagc tgcctgccgg ataaaatagt gacaaaccag gatatggaga aaactgttga
    31381 taccacagac gaatggataa gaacgcgtac aggaataagt gaacgaagaa tgacggatga
    31441 aaatactgcc acctctgatc ttgcaactat tgcggcaatg agggctctgg caagtgcaaa
    31501 tctcagtcct gaagaaatcg atttaattat agtttctaca gttactcccg atatgatgtt
    31561 tccttctaca gcctgcatag tgcagaagaa tattggagca gtcaatgccg ctgcttttga
    31621 tcttgaagcg gcttgtgcag ggttcttata cgggctttcc attgcagaca gctttgtaag
    31681 agcagggagc tttaacaaaa tacttgtaat cggtgcagag actctgacaa aaatacttga
    31741 ttttactgac agaaatacct gtgttatatt cggtgacggt gcaggagcag caatagtatc
    31801 ggaggtacct gagggatatg gaataatgtc tacatacatt ggggcggacg gaagaggagg
    31861 agatcttctg aaagtacctg ccggcgggtc aaggattcct tcaagtgttg agagcgttca
    31921 aaacagattg cattatgtga aaatggatgg caaagaggta tttaaatttg cggtaaagat
    31981 catgggggat gctgctgaga ttgcattggg aaaatgcggg cttacaaagg aggatgttga
    32041 cttttttata ccacatcagg cgaacataag gattattgaa gcctcagtaa aaagattaaa
    32101 aatagcccat gaaaaggtat atataaactt ggataaatac ggcaatatgt cttctgcttc
    32161 agttgcaatc gctattgacg aagcaaacag aaaaggtttg ttgaaggatg gagatactat
    32221 tgtacttgtg ggcttcggag gaggcctgac ctggggatcg gcagtattaa aatggaaaag
    32281 catcaggagg gattaatatg tttaagactt caatctgcga aatattagat atagaatacc
    32341 ccatactgca gggaggaatg gcttgggttg ctactgcaga acttgcggca gccgtatcca
    32401 atgcgggagg cctgggtata ataggcgcgg gaaatgcacc gggaagctat gtgagagatg
    32461 agataagaaa ggtgaaaaag cttaccgata aacccttcgg agtaaatata atgcttttat
    32521 ccccctttgt agatgaggtc atagatattg tttatgaaga aaaagttcct gttattacca
    32581 caggtgccgg aaacccggga aaatatattg cccggttcaa aaatatcgga acaaaggtaa
    32641 taccggtagt cccagctgta gctctggcaa aaaaaatgga gcaggagggt gttgacgccg
    32701 taatagcgga aggcacagaa tcagggggac atgtgggaga attgactaca atggcattgg
    32761 tgccccaggt aatcgatgcg gtaaaaattc ctgtcatagc agccggagga atagctgacg
    32821 gaagaggcct ggtgtcggta ctggctcttg gtgctcaagg ggttcagatg ggtacaattt
    32881 ttgtatgcgc ttccgaatgc acagcccatg aaaactacaa aaaggcaatt ataaaggccg
    32941 gagacagggg aacagttgta acaggaaaag ccacagggca tccggtaaga attctgaaga
    33001 ataagctggc acgggaattt gaaaagcttg aaaaagcctc tgcagccgtt gaggaatatg
    33061 aaaagctggg agtgggacgt cttagggctg ctgtagtaga aggcgacgca gactatggtt
    33121 cggtaatggc gggacagatt gcaggccttg taaaggaaat aaagcctgca aaggatatta
    33181 tagaagacat actgaatgag gcaaatgaag taatcagctc aattaatgga ttggtgaagg
    33241 aggaataaaa tgccgaaaat agcttgtatt tttccgggac aaggagcaca atacccgggt
    33301 atggggaagg aattggcaca aaattacaag gaagcaatga atatatttga ccttgccagt
    33361 gaaagactgg gaattgatat gaaaaagctt tgctttgagg gtgatgaaga agagctgaag
    33421 aaaaccgaaa atactcagcc ctcaatcctt actgtgagta tagcattgct ggaagtgctg
    33481 aagctcaagg gtattgaacc tcaaatagca gccgggttaa gccttggaga atactcggcc
    33541 ctggttgcat caaaggccat aagcttcgcg gatgcagtcg cattggtgaa aaagagaggg
    33601 aagtatatgc aggaggcagt tcccgcagga gaaggcacaa tggcggcaat aatgggcatc
    33661 gaaagggaat ccgtgatgga atgccttaag atggcaagcg gttatggtgt ggtagaagcg
    33721 gctaatttca actgcccggg acaggttgtc atagccgggc atacaagtgc tgttgaaaag
    33781 gcatgctcca tactgaagga aagaggagca aggaaggcaa ttatgcttcc ggtcagcgca
    33841 ccttttcatt caagcctgtt aaagcctgca ggggaaaagc ttgcagccga acttgaaaat
    33901 gtgaatatct cggataatga gcttcctgtc gtctctaatg taaatgcaca ggtaataatg
    33961 aacaaatatg aaataaaaag atgccttata gaacaggtaa gcagttccgt gctttgggag
    34021 gacagcatca gacggatgat tgatctggga gtggatacgt ttattgaagt agggcccgga
    34081 aggacactat ccgcttttgt taaaaaaatt gacaagagcc tttatgttca taatgtggag
    34141 aacatcgaaa ccatggagaa tactatagta agcataaggg agagattgga atgcaattaa
    34201 aaggcaaaac cgcagtcgtt acaggcggct caagaggaat tgggagggcc atagcacttg
    34261 aacttgcatc ctgcggagca aacgtagttg tgaactacac caggaattca aaagcagccg
    34321 atgaggttgt cgcggaaatt gaagcaatgg gttcgtcagg gatggctgta aaggctgatg
    34381 tttccatagc ttccgaggta gaaaatttgg tgaatgaagt attaaaaact ttcggcagca
    34441 tagatatatt agtaaataat gcagggatta caagggataa cctgataatc cgcatgacgg
    34501 aaaaagaatt tgatgaagtt atcaatacga acctgaaagg tacatttata tgcacaaagg
    34561 ctgtatccag agtaatgata aagcaaaagt cgggcaaaat aataaatgtt tcctcagtgg
    34621 tcggtatagt tggtaacgcg ggacagtcca actatgcggc agctaaagcg ggactcattg
    34681 gttttacaaa gtccatggca aaggagctgg caaagagagg catcaatgtt aatgcagttg
    34741 ctccgggctt tattcagacg gatatgacct ctgtacttcc tgacaatgtg aaggaggagt
    34801 ttcttaaaag tattcctctg atgcgtatag gaaagcctga agatatagca aaaacagtat
    34861 tattcctagt gtcagaatat tctgactata taaccggtca agtaattaat attgacggtg
    34921 gtatggttat gtaatattat catattataa aaaaggcttc tattgaagta atctaacaat
    34981 taaggaagcc ttttatgaaa cttgcttacg gggcatgata aattaaggtc gaatgaatgc
    35041 ccctactcgt tataaaatcc tgaagggagg tgaactggat gatttttgaa aaagttcagg
    35101 agaaagtagc tgagcagctt ggtattgaac cagaagagat tacattggag tcttccttca
    35161 ttaatgattt gggtgctgat tcacttgata tagtagagct gttaatggcg ctggaagaag
    35221 agtttgatat agaaatacct gatgaagaag cagagaagct gtctacagtt ggtgatgtgg
    35281 tggaatacat aaaaagcaat aaataaaaaa tgtcccgata gcgggacatt ttcattgcta
    35341 gatatgtaat gtattctgtt cattagtaaa tatatatatc atcattataa ggagaggaaa
    35401 cgattgaaca agagaagagt ggttgtaaca ggaataggag ttatttctcc catcggaacc
    35461 ggcagggaga aattctttaa aagcctgaaa gaaggcgtaa gcggcgtggg tcatataaca
    35521 aggtttgata cggaaggctt tgacacaaaa atagccgccg aggtaaagga ttttgatccg
    35581 tccgggtact tcgacaggaa ggaatgcagg agaatggaca gattttcaca atatgcaatt
    35641 gccgcttcca aaatggctgc ggaggattca aatattaata ttgaatcatt ggacagcagc
    35701 aggttcggtg tttgcatagg ttcgggcata ggcggaatgg agaccatgga agcacagtat
    35761 gatatattga gggaaaaggg gcccggaagg gtcagccctt tcatgattcc catgatgata
    35821 tccaatattg ctgcgggaaa catttccatt gccttgaatg caaagggccc cagcattaca
    35881 gttgtaacag catgcgcatc tgctaccaat gctatcggtg aagccttcaa gattattcag
    35941 agaggcgacg ctgaaataat ggttacagga gggactgaag cctctataac acctttatct
    36001 ttagcaggtt tttgttctat gaaagcttta tcaacgagaa atgatgaccc aaaagcggcg
    36061 tcaaggccct ttgataagga tagagacggt tttgttatgg gggaaggagc gggaatgctg
    36121 attattgagg agcttgaaca tgcactgaaa agaggcgcgg taatatatgg ggaaatagca
    36181 gggtatggcg ccaccggaga tgcatatcat attactgcac cggctcctga cgctgacgga
    36241 gcatacagag ccatgctcat ggccgtcaat gatgcgggaa taaatgcctg cgaactggat
    36301 tatataaatg cccacggaac ttcaacggac ttgaatgata aactggagac cctggcaata
    36361 aaaaggcttc tcggagaaga agcaaaaaaa gttgcagtaa gctctacaaa gtcaatgact
    36421 ggacatctcc ttggggcagc aggagcaatc gaggcaatag catgcttgat ggcaataaat
    36481 gaaggagtga ttcctccgac aatcaactat acaactcctg atgaggagtg tgatttggac
    36541 tatgttccaa ataaggctgt caacaggaag gtagactggg ctatgtcgaa ctccttgggc
    36601 tttggaggcc ataatgccag tattatattg aaaaaatatc aataagtgat attataggga
    36661 ttgggaatac tcgttggggg ttattgccaa tccctatttt tatttcaaaa gcgcatatgc
    36721 ttttgccaat aaacgaaaag gcagttggga ggaatggaaa tgtccgttat cgatgaaaag
    36781 cggctgaagc agttaaatga atttgagagt atcattggat attccttcaa gaatatcagc
    36841 atattaaaca atgcccttac tcacagctct tacgtggctt caaggtcaga tcatcatgaa
    36901 cacaatgaga ggcttgaatt tatcggagat gctattcttg acatggtaat aagcctatat
    36961 ttgtataaaa agtgcaagaa cctgacagaa ggaagcctta ccaggttcag ggcggggatt
    37021 gtgtgcgagc agtcactgta taacgcatca aacaacctga agcttgggga atacctgctg
    37081 ttaagcaaag gtgaagagaa tacgggaggc aggaagaggg tttcaatact ggcagacgct
    37141 tttgaggcag ttattgcagc tatttacctg gacggcggca taaaaaaggc aaaggagttt
    37201 attatgaaaa atctcgagac tactattaag aatgcagttg agaataagtt attcagtgat
    37261 tataagtctt tcctgcagga gtatgtccag aaaaacaatt tgggtaagct gagttataag
    37321 ctggtcagag aggaaggacc ggatcatgat aagacctttg aagtggcatt gtatttaaac
    37381 aatgggatta taggaagagg agagggccgc agcaagaagg atgcgcagca gtctgccgca
    37441 agagatgcca tagccggcct gggtgtaagg aatgtttaag aagcattata tcattccgat
    37501 ttttgtacct cataaaggct gcccccatga ttgcatattc tgcaaccaga aaaaaatcac
    37561 aggccaaatt gagaaagtga cagaaaagga cgtaaaggat aagattgaag attatcttga
    37621 tatgatacat gaaggcggca gccatattga agttgcattt tacggcggga gttttacggc
    37681 aatccctctt gaataccagg agcagctgtt ggatgcggct tttgtatatg ttaaagccgg
    37741 aaagataaca ggcataagag tttcaacaag acctgactgc atagatgcaa ggatttcgga
    37801 gaatctgagg aaatatggag tacggacagt ggagcttgga gttcagtcaa tggacgaaga
    37861 agtacttaag ctgtgcaaca ggggccattc tccgggggat gttacaaacg ctgtcgggat
    37921 tctgaaggaa ttcggctttt cagtcggtgt acagatgatg ataggcttgc ccggagatac
    37981 tgaggaaaag gctgtgaaga ccgcaaaagc ccttatcgca cttaagcccg atatagccag
    38041 aatatacccc gctctgacaa taaagaacac gtatatggaa gaaatgttca tcaggggaga
    38101 atatcaaccc ctgacccttg atgaagctgt tgaattatca aagaagctgc tgatactttt
    38161 tgagagtaac ggaataaatg taataaggat agggcttcag cctacagaga atataaattc
    38221 ggggaaggat gtcgttgccg ggccctttca tccttcaatg aggcacctgg ttgaatccct
    38281 catatacagg gacatgttgg cttatttatt ggaaaaggaa ggagaaaaga agaaaattac
    38341 cttgggaata aacccgaaaa atgtttctga attggtgggg cataagaaaa gcaatattga
    38401 ctacataaaa tcaaaatttt ttatcgacaa aattgaaata tttcaggata ataatgtaga
    38461 agccggtact atggtgctaa ataatggtaa aaattccgtt attatgtcaa agaaatacta
    38521 ttattccatt ttgtctaaat aaaggtgttt ggagatttat atagaatata tataatcaac
    38581 tatacagaca tatttaggag gtattccaaa atggaggtac taaaagtatc agctcattca
    38641 caaccaaaat cagttgccgg agctctcgcg gctgtactac gggacagagc gacagctgaa
    38701 atacaggcag ttggtgcagg tgctgtaaat caagcagtaa aagcaatagc aatcaccaga
    38761 ggttttgtag caccaaacgg tatagacctg gtagtagtcc cggcattttc agaaattcaa
    38821 attgacgggg aggatagaac tgcaataaag ttcatagtgg agccaagata aattattggc
    38881 taattttctg aaagcctata tcataacgga tattgggctt tttgttttgc cctgtgacct
    38941 atatcctata gcatataagg acaaggtatg ttaaaatata ttggtaaaaa tctgttgttc
    39001 gggagtgacc tgatttgtat ttgaaacagc ttgaggtaca tggttttaaa tcctttgcgg
    39061 aaaaaattga acttagcttc ggcaatacaa taaatgccat tgtcggtcct aacggaagcg
    39121 gcaaaagcaa tatttccgat gcaataagat gggtattggg agagcaaagt gtaaaggcct
    39181 taagaggaaa caagatggag gatgttatct ttgccggcag cgacagtgaa aaagccctgg
    39241 gatttgctga agtttcctta tgcattgaca acagcgacag aaagataccg gtggattata
    39301 cggaaataaa tataatgaga agaatgtacc ggtccggtga aagcgaatac tttattaata
    39361 agacttcatg caggcttaag gatatctatg agctgttcat ggatacagga atcggcagag
    39421 acggttactc aataatcggg cagggaagga ttgacgagat actcagcaat aaatcggagg
    39481 acaggagaaa tgtgttcgaa gaagccgcgg ggatagtaaa atacaagaca aggaagcatg
    39541 aagccgagaa aaagctggag cagacaaatc agaacatagt aaggctgggg gacataattg
    39601 aagaactgga gtcccagttg gggccgctta aggagcaggc agaggtcgca aagcgctaca
    39661 gaagcattat ggaagaattg aagtcaattg aaataaatat tatgctgaac agcattgata
    39721 aattccgcag caggctttcg aatgcgaagc aggaggcaga gaacatacag agccttatag
    39781 atgccaagaa ttcctctgcc cggcagctgg agcataagca aaatgaactt aaaagaattc
    39841 tgaatgatat agagacaagt attgacatgc taagcgagga tattcaccac cttgaaaaca
    39901 gcaaggaaaa aatggaaggg gaaagcaatg tccttcaggt aagaataaat aatcttgagg
    39961 agaacacctt aaagctcggt aaaagcggcg aagagcttga gaatgaactt acagagtcaa
    40021 ggcaaggtat tgaacagaac agactgaagc ttgccgagct taatactgca tatattgagg
    40081 tatacggtaa aatcaatgaa agaactgccg actttgaatc caactttaag gccataaatg
    40141 accttgaatc ggatttaagt gataaacagc agaaattcat aagcctgatt aacaattatt
    40201 cggagctgaa aagtaaaata aacagcacta atatattcat agaaaatatg aataagagga
    40261 aaagccaggt agagggggat gtgaacctgc ttgaactgca gcagcaggat aaaaagaagc
    40321 agtatgataa atcccttgaa gaactggatg acttcggaag aaaaattgaa gactgcaaaa
    40381 cacagataga ggagataaaa gggaagaaac aggaatgcga gagtgaaata ctgttgctgg
    40441 aggataaggc agggaagctg aaggcggaga aagaagccat gttttcaagg cttaaggctc
    40501 ttgacgatat ggaaagggat cacggcggat acagcagggc agtaaaggtg cttatggcgg
    40561 aatatgacaa ggtaacaaaa ggtttctgcg gtgtcgtagg tgaaatgatt aaagtaccct
    40621 ccaagtatgt aaccgctatc gaaattgctc tgggaggctc cgtgcaaagc ataattacaa
    40681 atacggagaa tgatgcaaag cttcttatag aataccttaa ggagcataag acggggaggg
    40741 caacctttct gcccataagc tcagtaaaac caagattttt caataaggct gaagaaaagc
    40801 ttttcaatat gaagggattc gtgggaattg catccgacct tatcgaatat gacctcaaaa
    40861 tcgacagtat tatgaagaac ctgttgggca gggttgctgt tgttgaagat attgatgcgg
    40921 caataaagct tgccagggaa gcaggatatg attataaaat agtgactctt tcaggagagg
    40981 taataaacgc aggtggctca ataacagggg gaagccttca gtcttcttca agctctgttc
    41041 tgaccagaaa aagtgagata gaagctctga aggtcaagca taaagaaaag cagcaggagc
    41101 ttgatgaact cagccggaag cttggggaat tgaaaaaaca agcagcggat atgggcgccg
    41161 acatagagtt caagacggaa atacttcatt ccctgcagat agacattaat aattacagga
    41221 acaggtgtga caatctgaaa aacgaaatcc aggttattca ggacagaata tcaataaacc
    41281 taaaagacct tggagatctg gagaaggagt ataacgaaac aaataagata atttctgaaa
    41341 ataaggaaaa ggcagaggcc gtcgaaaagg aaaaggaagc tgttgaggca cagatatcgg
    41401 agcttcagga gcagatgaag cagttcaagg aagtaaggga tgaatataac gatgagataa
    41461 cagagctgaa aataaaactg gcatctgttg agcataataa gaagaccgtt gaaaactcaa
    41521 taaaaagcat gggggagaga gtaacagaat tagaggagga aataaaatca aacaatgctg
    41581 aaatagaaag acagcaagcg gaaataaaag cattgtcata tgatttggaa gaaaagaaaa
    41641 ctattataga aagaactgta agagaggctg aagaaaagca taagcttcat aatgacaagg
    41701 agaaggagaa atctgccata tgtgaagatt tgagcaagct tgaagcagat ataaagaatt
    41761 acagcaatga tatagctgtt ttgcaggcag accttcataa agctgaaatg cagcatgcca
    41821 gaactgagat agagctggag agcattgaaa tgaggctgct ggagacctac gaaatgaatt
    41881 acagcaaagc ggaaagctac agggatacaa gcttgagtct tacatgggct gcaaagcact
    41941 gtgaggaact aaaaaatgat atcagagaaa tgggcactgt aaatgtaaat gcaattgagg
    42001 aatatgaaaa gctcaaagag cgccatacat tcctcagcgt tcaggtggag gatctctcaa
    42061 aagcccggga aaaccttata gccgttattg ccgaaataga agaaaacatg aagcttcaat
    42121 tcattacgga attcaacaag attaatgaaa atttcaatat tgtgttcaag cagctttttg
    42181 gcggaggaca tgcggaattg gtattgacgg atatggatga tgtactgggg tcagggatag
    42241 aaataatagc caaacctccg ggaaagaagc tgcaaaacct attgctgctg tcagggggag
    42301 agagggcgct gacagctata gccctgttat tcggtatact caaaatgaag cctgcacctt
    42361 tctgcgtcct ggacgaaatt gatgcggccc ttgatgatgc caatgttgac aggtttgcag
    42421 agttccttgt aagcttctcg gcgaccacac agttcatcgt tgtaactcac agaaagggaa
    42481 cgatggaatg tgcggactgc ctgtacggag tgagcatgga agacagcgga gtatccaaac
    42541 tggtatccgt aaggcttgaa gataaagtaa gttaacacgc aacagaggaa gaatgcgacg
    42601 gggacacggg ggacggggac atggggacgt ttcgcgtgtc ccactggaag tgggacaggg
    42661 gaaacgtccc cgtgtccccg tccccatgtc cccgtcgcat tattcttcta atacgaaata
    42721 aaggagaatt tgaatatggc gggattattt aaaaagctgt ttaacagagg tcaggagaac
    42781 gagcaggaag aattacaggc agaagagcag gagcagctga ccgatgaaga gatagaagca
    42841 ataaatgaag aagaaattga aaatgaggaa acggtcgaaa atgagcagtc ttccatcttc
    42901 tcaaggctta agaatggact tgcaaaaaca agaaataata taaccggtaa gctgaacggg
    42961 gttcttgcaa gctttaagag cgtagatgag gagctttttg aagagctgga ggaaatattg
    43021 attacctctg atgttggtgt ttcaacaacc ctgatgatat tggacaaact aagaagcaga
    43081 gttaaggagc agaatataaa agacccctct gaggtaaagg aacttctcaa gcaggagata
    43141 cttgccatta tgggagaaag cacggagagg cttggggaac agactcctgc tgtaatactt
    43201 gttattggtg taaacggagt aggtaaaaca acatctatag gaaagattgc ttcaaaaatg
    43261 aaaaagtccg gcaagaaggt aattattgcc gcagcggata ccttcagggc cgctgcgata
    43321 gaccagcttg aaatatgggg caaaaggatc ggagttgatg taataaagca tcaggagggt
    43381 gctgaccccg cagctgtgat atttgatgca attaactcgg caaaatccaa gaaggcagat
    43441 atacttatat gcgatacagc cggcaggctg cacaacaaaa agaacctgat ggaagagctg
    43501 aagaagatat caagaataat agaaagagaa ttcccggaag cgtacaaaga gactcttctc
    43561 gtacttgatg ccactaccgg acagaatgca atttcccagg caaagctttt caaagaggca
    43621 gcggacataa acggattggt gcttacaaag cttgacggaa cggcaaaggg cggaataatt
    43681 attgcgataa agtctgaatt ggatataccg gtcaagctta taggagtagg agagaaagtg
    43741 aaggatctgc aggaattttc agcttcggac tttgtaaatg cattgtttga ggaagacgaa
    43801 taaatttgtt gacaaatata taatgttaat ataaaatatc aatgtaaagg tcataacctt
    43861 tacattgata tttttataga agcaatgagg tagaaatatg tacgacaagc ttgtacaaat
    43921 agcattgttg tttgattttt acggtcagct gctcacagaa aagcagattg agatagttga
    43981 catgtattac aacaatgacc tctctctggg cgaaatatca gagcaacagg gcataagcag
    44041 gcagggggtt tatgacactt taaagagggc tgaaaggact ctttatcaat atgaggaaaa
    44101 acttggcctg gtggatagat ttctgaagca gaagcaaaat atggagaaga taattgaaat
    44161 gcttgaggaa ttaataacag aagacggata tgacaggaaa aatattaaag ataagattaa
    44221 tgaaataaaa gagtatattg gcaatataat atgagtaatt attgaatgca ttgaccctgc
    44281 gcaggctgaa agatatacag cggggcaaga tgaggtgaga tgtttggcct ttgaaggttt
    44341 agccgaaaaa ctgcagaata cgctgaaaaa gctcagagga gccggaaaat taagcgaaaa
    44401 agacgtaaag gatgccatga gagaggtcaa aatggcactc ttggaagctg atgtaagctt
    44461 taaggtggta aaggaattca taacaaaagt gacggaaagg gcggtagggc aagaggtatt
    44521 ggacagcctg acccccggcc agcaggtaat aaagatagta aatgaacaga tgacagagct
    44581 tatgggaaac accaacagca aaatcatctt ttcaccgaaa cctcccacta ttcttatgat
    44641 ggttggcttg cagggagccg gaaagacgac tacctgcgga aagcttgggc tgctgcttaa
    44701 aaaacaggga aagaatcccc ttttggttgc cggagacgta tacaggcctg cagcaatcaa
    44761 acagcttcag gttgttggca gccaggtggg tatagaagta tttgccatgg gcgataaagt
    44821 gagcccggtg gatatatcaa aggctgcggc tgcacatgca ttaaaaaacg gcaacgatgt
    44881 tgtaataata gacacggcag gacgccttca cataaatgaa gagttgatgg atgaactggc
    44941 ggatatcaaa gaaaatgtta aacctaccga gatactcctt gttgttgatt caatgacagg
    45001 acaggatgcc gtaaatgtcg ccgaaagctt caatgaaaag cttggcattg acggtgtgat
    45061 actgaccaag ctggatggag atacaagagg cggcgcagca ttatcagtaa aggcaattac
    45121 aggaaagccc atcaaatttg ccggtatggg cgagaagctg aatgaactgg aaccttttca
    45181 tcccgacaga atggcttcac gaatcctcgg aatgggagat gtgctgagtc ttatagagaa
    45241 agcccagtct gcatttgatg agaagaaggc cgttgagttg gaaaggaaga tcaggactca
    45301 gcagtttacc ttcgatgatt ttcttgacca gcttcagcaa atgaagaaga tggggccgct
    45361 aagctccgta cttgaaatgc tcccgggaat gaacccaagc aagctcaagg gcatggaggt
    45421 tgatgacaaa gagcttgtaa aggtagaggc aataatcagg tctatgacaa aaaaggaaag
    45481 gcataatcct gatataataa acggcagcag gagaaaaagg attgccgcag gcagcggaac
    45541 cagcatacag gatgtcaaca gagtcatgaa gaactttgaa cagtcgaaga agatgatgaa
    45601 acagtttgcc ggtatggaaa aagatatgaa gaagggtaaa atgaaaatgc ctttctttag
    45661 ataaaaagca ttatgagata attcagtttt gtaaggaggt gaattcaatg tcagttaaaa
    45721 tcagattgag gagaatggga gcaaaaaaag cacctttcta tagattggta gttgctgatt
    45781 caagatctcc gagagatggc aagttcatcg aggaaatcgg gtattataac cctatcacag
    45841 aaccggcaga aatcaagata gatgctgaaa aagcaaagaa gtggctggat tgcggggctc
    45901 aaccatcaga cacagtgagg tcgcttttaa agaaaagcgg aataattgaa ggttaatcaa
    45961 acggaataaa tttcctttag gagtgttgca attgaaagag ctggtagaag taatagcaaa
    46021 ggcccttgtt gaccaccctg aaatggtaaa ggtaagagaa acagaaaatg aaagatcaat
    46081 tgttttggag ctgacagttg ctcccgagga catgggtaag gtcataggaa ggaacggaag
    46141 aattgcaaga gcaataagaa ccgttgtgaa atccagttca gcaaggctgg ataaaaaggt
    46201 aatagtagag atactataaa tattctttta tttttccata gtatatgaaa gcacgctttc
    46261 atatactatg ggcattaatt ctttttgggc ttttgcctga ttatttgcgg gagtagatga
    46321 aaatgctgga atacctgagt ataggacaaa taataaacac acacgggtta cggggggcag
    46381 taaaagtata ccccctgact gatgacatca gcaggtttaa aaagctcaaa gaggtttatg
    46441 tggaagaaaa cgacgggatt gtgaaataca aagtggaaag cgtcaaattc ttaagcagtg
    46501 cagttgccgt aaagcttaag ggtattgact cggaagaagc ggcaaataag ctgagaggct
    46561 tttatattaa ggttgacagg aaatcagccg ttaagcttcc caaggatacg tatttcatat
    46621 gcgatttgat agatttggaa gtgtatgatg aaaaaggcct gcttataggt actgtcaagg
    46681 atgtgctgca gaccggaagc aatgatgttt atgtaattga atcttccggg aaggatattt
    46741 tgatacctgc ccttaaggat atagtcaaga aaattgattt ggaaaacaaa aaaatcttgg
    46801 tagaaatgcc agagggtttg atttagatga agatagatat actgactctt tttcccgaga
    46861 tgtttgaggc cgtattgaaa gagagcatta taggaagagc attggaaaag cagctgatag
    46921 agcttgaatt tcataacata agggattatt cggataacaa gcacaggaag gtagacgatt
    46981 atccctacgg cggcgggctg ggaatgatta tgcagtgcca gccgatattt gccgcagtgg
    47041 aagcaataag cgcaaagctg ggagaaaagc ctcacatcgt acttatgagc ccccagggaa
    47101 gaacctttga ccaggcaaaa gccgaggagc taagcactca taagtatctt acaatattat
    47161 gcgggcatta tgagggaata gatgagagag ttaatgaagc acttgtggat gaggaaattt
    47221 caatagggga ttttgtattg accggcgggg aaattgccgc catggcagtg gtggatgcaa
    47281 cctgcaggct tattccaggt gtacttaagg aggatgcctc ttatacggag gaatccttca
    47341 accggggctt gctggagtat ccgcagtata caaggcctcc tgtatttatg ggaatgccgg
    47401 tgccggagat tttgctttcg ggccatcatg aaaatataga aaaatggaga agatatcaat
    47461 ccttgaaaag gacttgggaa agaaggccgg atctgcttgc agaatcagaa ctgtctgaag
    47521 aggacaggag gcttttggat aagataaaag aggaatgcaa ataattctct taagcacttg
    47581 catcaaggct tgccttatgg tatactttta acgttattac gggcgttccg ctgtcaaagc
    47641 accggtgcaa tgaatatgaa ttgcatgaat tataattgta catgaacgtc tatgatggaa
    47701 ggagggacat tgtgatggat ttgattaaag ttatagaaag cgaacagctt agaaatgata
    47761 tacctgtttt taacgtagga gacactgtta aagtacacgt taaggttaaa gaaggaaaca
    47821 gggaaagaat tcaggtgtac gaaggtgttg ttatgaaaag acagggtggc ggactgtcag
    47881 agacttttac cgtaagaaga atatcttatg gtgtgggtgt ggaaagaacc ttccctttac
    47941 actcacctaa actggacagg attgaagtcg taagaaaagg taaggtaaga agagccaaaa
    48001 tcaactactt gagaggcaga gtaggtaaga gagctaagat aaaggaaata agataaaagg
    48061 ggcttaatag ccctttttgt ttttaattgg agtgatgcat atgaacattc aatggtatcc
    48121 ggggcatatg gccaaagcca agcgaaagat agcggaggag ctgaagctgg ttgatgttgt
    48181 aatagagctt ctggatgcaa gaatacccat gagcagcagg aatcctgaag tagatgagat
    48241 tgtcgggaat aagaaacgga taattgtgct gaataaaagc gatctggctg attcttcggt
    48301 taatgcaaag tggttggttt attttaataa ggaaaacaca agggcgatat tggttaattc
    48361 cttgaaagga aatggactca aggatgtact gtctgcttca agacagctta tgaaggaaaa
    48421 gcttgacagg ctgaaaagca aagggctgct ggttaagaca acaagagctc ttataatcgg
    48481 cataccgaat gtcggaaagt ctacgttcat taacaggctt gcgggaagaa gcgtggctca
    48541 gactggagac aggccgggag tcacaaaatc gaagcagtgg ataaaggtga gtaacgagct
    48601 tgaactgctg gacacaccgg gaatcctttg gccaaaattc gaggataaaa gggcgggaat
    48661 gtatctggcc tttaccggag cgataaagga tgagatattg gacgtaaatg aactggctca
    48721 gaatctgctt caggtattgg cagagaagtt tccggaaaag ctgaaaagca gatataagct
    48781 ggaagacata ccggaaggaa ttgcaccgga agagcttatg aaaagcatag gcagaaaaag
    48841 aggctgcata atagcgggag gagaagttga cgtgctgaga gcttccgcaa tgctgctgga
    48901 tgagttccgg ggagggaaga taggggctat atcattggaa tcaccggaag atttttaggg
    48961 taatggcgat aaggggcata aataatggtg ccaaaaatga ttatgtatta gaggggttta
    49021 agcgagctta ggcccctttc gcatttccag aaattacttc gttttcagcg tatcttatgg
    49081 tataattgga gtatcataag ccttggaaat acggaggtta tgggttttat gcctaaaaaa
    49141 aacgatgact atataagcgt attaggcggt tgcaacatgg acattattgg ttttccttac
    49201 aagaattttg ttcctgaaga ttcgaatccg ggccggatta aggctgcttt agggggagtg
    49261 ggcagaaaca ttgccgagaa ccttgtttgt ttaggcatcc ctacaaagct gataagtgca
    49321 atcggcgatg atatctacgg aaagataata ctggagcatg ccgataatat cggccttgat
    49381 atgaagaatt ctctggtatc gaatcaaaca actacatcca catatttagc tatactcgat
    49441 gaaaaaggag atatgaaggc agctgtttcc aatatggatg tactggatga aattaccatt
    49501 gaattcattc aagaaaagaa gcaagtgata gagaattcga agatatgtgt aatagatact
    49561 aacatgccaa ccgacatcat taattatgtt ttagataatt ttaaaaacac ggattttttc
    49621 ctggatactg tatcaggaac aaaagcccaa aaggtaaaaa acaatatcgg agcattccat
    49681 accataaagc caaacaggat agaagcagaa attctttcgg atattaaaat agacagctta
    49741 ggcgatatga taaaagcctc agagtatttt ctcagccggg acgtaaagag agtctttatt
    49801 acattagggg ctgagggtgt gttctataat gatggaaatg ttcataaatt gatacctagc
    49861 cccaaaataa aagtaatcaa tgcaaccggt gccggagatg cattcctggc tgccttggcc
    49921 ttttgccgct tcaaaaatta cggtattgat gaaagtacac gtttttcaat ggcagccgca
    49981 attttggcat taagctgcga aaaaacaata aatcctaata tttctgaaaa aaatgtaata
    50041 aaaaaaatga aggagatagg attatactga ataaatatct ggatatcaat ccggaggcag
    50101 aaaacgctta aatgaaggtt aaagaacttg cagccggcgc aagctttgat tccaatatta
    50161 agcttgttta taataaagtt agggttggag cacatatcag cagatcacac ttttctatct
    50221 gcttcattac ctttcttagt tctccatttg cttttgcatc ggctaatccc aatgtgacct
    50281 taccccaaat tttgtaccac gctgaatgtt agtccggccg attctggggc gttttcattc
    50341 tttagttttt gagcatattc atctggtgtc agattaccca gagaactatg tggacgcaag
    50401 gtattgtact cgatgcgcca tgattcaata atttctctgg cttcatatat gttcttaaac
    50461 caatgttctt caaggcattc ctcccggaac tttccgttga agctttctat gtaagcattt
    50521 tggacgggtt tccccggttc aataaataaa tgattgattt tcttctcgta tgcccactca
    50581 ctgagggcaa cactagtaaa ttcagggcca ttatccgtaa gtacctcttt tgggtaaccc
    50641 ctaaagaatg caatacggtt aagaactgat gcgactcgct gacctgttat ggatgtatct
    50701 acttcaaggg ctaaacactc cctagttact tcatctatta cagttaagac tttaatacga
    50761 cgtccatttg ctgtagcatc agatacgaaa tcaaatgacc atcttgaatt aggagatgca
    50821 gccagctcag gtttaccacg tttttcagaa ggagatttct tctttctctt gcgcagcgtt
    50881 aaaccagctt ctttgtataa tcgatatgtt ctcttatgat tgattttaaa accttctctg
    50941 cgaagaagta catgaagacg cttatatcca aagcgtttcc atcgtacagc aagttcgcca
    51001 agacgtttgg taataacctc attttcctca ttcttggctg gaacatatcg tttaaaggtc
    51061 ctatttatgc tgagaagcct gcacacgcga cgttcactga gtttaaactc tttctgtagc
    51121 tcattggcta tttccttttt atgggcaggc tgaattcgtt tttttcgact acccatttaa
    51181 gtgcctggtt gtccaatgta aggttagcaa caagctcctt aagccttcta ttttcatctt
    51241 caagttgttt taggcaacga gcttcactga tctccatacc gccatatttg gatttccagc
    51301 gataaaaggt ttgctcggta atattatgct ggcggatcag ttccttaact ggaacaccac
    51361 tttcatgctg ctttaagaca ctaataattt gttcttcggt aaaattcttc ttcatattct
    51421 tccctcctgg ataattttat tatatcatcc agactaactt tgagggtgga tcaatttaaa
    51481 gggagaaggt caaatggcct aagtttaaac atgattaaag attcctttac aaaaagatta
    51541 ttccgtttct aaagcagtta atactaaaaa acgttgacaa gtttccgaaa acagatacta
    51601 taaaaaataa tcaattccat caactcgatc cacgtggaga cggtggtatt aatgtcacgt
    51661 gcaaaccctt gaaaatacag tgtatcagag tgacatccat aatgaccact cggcctaaag
    51721 tgtgttttgc gtttgtggaa acatatcggc agataagaaa atgaacttag ggttgagtgg
    51781 acggatttga taacatctgt agagttgtga tgaaaggcta gatgttaaaa atagccgtac
    51841 aatgaagatg ttattatttg atataaacag aataaaagta gtatagagcc gacaataagc
    51901 ggcacaggaa gtcgagtcaa taaaattctg ataagatatg attttaatga gggtgagaat
    51961 atggattggg ttaaacagtt gaatgaatca ataaactata tcgaagaaaa tcttgtgggt
    52021 gaaatatcct atgaagtaat atcaaaaatt gcaagatgtt ctgtttataa ttttcaaagg
    52081 atgttttctt atattgcaga taagccatta tctgagtata taagaaatag gcgcttaacg
    52141 atggcagcat ttgatttatt aaacagcaca gacagaataa ttgatatcgc aattaaatat
    52201 gggtatgagt cccaagatgc gtttacacgc gcgtttaaaa attttcataa tgtacttcct
    52261 tcaagtgtca gaaacgaaat agttcagctg agatcctgtc caagactctc ctttcaaatt
    52321 tcaataaaag gagaaaatca tatgaattat caaattgaac aatggcctgc attttatgta
    52381 atgggaatct cgcacaaaat aaagacatca actgcatttc aagttatacc gaagatatgg
    52441 gaagaagcat ggaaagacgg aaccatgcaa aggttcatag agaattttcc ggattacaga
    52501 cctgctggat ttctaggaat tgctgctagc ggacaatggg gagattctga agaaatgaac
    52561 tatataattg ctgtcacaag ccatgttgat atacatgaat gtaaacacat tcctgtactt
    52621 gaaggaatgc aggaattctc ttatcctgct gcaacatggg cgatttttga ggctaatgga
    52681 gaactacctg acgcaacgca gaaggtatac aagcagtttt atactgagtg gcttcctaat
    52741 tcgggctatg aactggctga tatgccagtt atcgaatgtt atatgcagga aaaccgtcag
    52801 gaagtttgga ttgcagtgat aaagaaatag agaagtcgga gcagctatgg aatattcatt
    52861 taagtcaggc cattataatg aagggaatag gatatttata aaagtaccat tcaatgtttg
    52921 ggaacttgtg gtaagaaagg taatatacct attgaagctg ttattgagaa tataaccttt
    52981 gaatgcaagc taatcccaaa aggaaatgga gaatatgtgc ttccattaaa caaggatatc
    53041 tttaataaat tcagcgtaag tgatgaattc aatgttaaat ttactatatt aaaacaatta
    53101 actagaataa ataataacag cccatatagt aaagaaaatc caataaggca cattgatagt
    53161 ttcaattact tgaagcagcc ctcaattggc agttgtgggc aaacatgcct agctatgcta
    53221 gccggtattt ccgttcaaga ggttataaaa ataatgggga gtaacaaatg gcaggcaagt
    53281 ataagtaaag tacttgaaac ccttgactat tttggccttt catataatat acctgtctat
    53341 actcatggaa aaaaggtaaa tttaccaaag tgttgcatag taaatgtaag tggcagcgac
    53401 aaaaatcatt taatggttta ttatgatggc cttttttatg atccggtttc aagagttaca
    53461 gatgattatg attatgagaa cgtaatcaac tttatagaga tatatgatta atcaatgaat
    53521 aattatggtg agcttgtaaa aaatacttgc ttaatattaa tttgaattcc aataatgtat
    53581 attgggttat tgtacaaact ccaaggcttc atatttttcg ggtagatatg ttccctaaaa
    53641 cctcaatact attgatatgt ttactcaaac ctcgaaaacg cttaaaaacg gttgatatgt
    53701 tcccatggac gctgacatca ataatgaata ctcaacccac gttgagtgtg ttgtgttaat
    53761 agagaaaata taggttgaaa gatttacaag ttaatatttt agtgtattta attcctttaa
    53821 actattagtt tagaggaatt ttgcgctgaa ttgtgctatt gcactttaaa tacaacccca
    53881 aaacggtttg ctgtgaatga taaaattaag gagaccaaaa acgagcgtaa aaaggatacc
    53941 acctt
//
LOCUS       DAOOAB010000012        51924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_789_length_51924_cov_15.945864, whole genome shotgun sequence.
ACCESSION   DAOOAB010000012 DAOOAB010000000
VERSION     DAOOAB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 51924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51924
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_789_length_51924_cov_15.945864"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(532..4113)
                     /locus_tag="PKU88_04315"
     CDS             complement(532..4113)
                     /locus_tag="PKU88_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX68540.1"
                     /translation="MRPRYLELEGLQSFKELQSIDFDRLGETGLFGIFGPTGSGKSTI
                     LDAITLALYGNVQRANRGTQGILNLSSANVRVSFTFDLVKDKKRKSYKVEREYRRKKD
                     SENSIESRTARLIELNPSGDAVIADKPGEVNEAVIELIGLQFDDFTRSVVLPQNKFQE
                     FLLSAKGDKTKMLERIFYLEEYGRELTEKLNRRLGRIRNRLSGVERALSVLGDISDAS
                     LKDSEAGLIAAEKHRKSVAEALRAAEDEYYGAKEVWELSAEYRETEEKYQELLAGRAE
                     IDLMRISCKGAEAARSLMDRINDIKKTGGELENVTKELERLNTSLCELEGQLEQGQAE
                     LESATKARQEKIPLLIGHRTKLEKVLKTKSELEKIEEVLRKLRNEHVILKKKVDDWNE
                     TIAKQKSGLEGLLKEAAGKKARTEAIKVEAGCRDKIRYGASLEEDLERLQKEKEKQKA
                     KYEEMADIIILQEQEIESFKETGLKLLKEKEAIQSSYEQHKKSKKWDRNDIHKEETKY
                     YSIRSIVDALKLKYKDMEQLRQKELENGKQFEALDAQLEELSEIESLRLLLGERKKEQ
                     EGHAAFLLAQNLKENEPCPVCGAVTHPNPAAHRKNGHGTEAEAVKELQDVIRKLETQL
                     RAMENKGIKLGEQRNNLKKQQEELLSDIMKKQEEQAELIERLPEDYKGLTVPEIERAL
                     GKNGEEKQEKLKDIELWEKKLAELEESHKKIAEEYNRYQVENSSMASRLEADSKHLKD
                     LEKAYTEAKEAWAEKERTYTAAVKDTGIGSMRDELRRIQEKDKEAENTYEELQIIEQN
                     ISELRKEIDKAEEARKLDMEKLSENTAEGRGFKYQKEEKEKEIHDFIGDKDIENEILK
                     TEDEISFLTKGEQKAQQNVNTLRERYEKEVKDKKVLEKQSEIYRNKLSDDRKKLDKEL
                     ADKGFSSLEEVEEAFMTKEELEERVKIIKEYEKAESSITAHKALIEKKLSGRSITEEL
                     WQEINRRYEELKLEKENSIARYESEKNRLETIKSNYENWLLLKKELKEYSKKKEHLEL
                     IKSLLKGNSFIEYISEERLRYIAREASETLGILTRHKYGLELDTENGFVIRDDANGGI
                     RRPVTTLSGGETFLTSLALALALSSQIQLKGQSPLEFFFLDEGFGTLDSSLLDTVIDA
                     LERLSTRERVIGLISHVPELKSRITRRLVVEAPMVNGSGSRVIMEKA"
     gene            complement(4094..5350)
                     /locus_tag="PKU88_04320"
     CDS             complement(4094..5350)
                     /locus_tag="PKU88_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPX68541.1"
                     /translation="MRILHTSDWHLGKTLDNINRLDEQRQFIDELCGIAESESADLVL
                     IAGDIFDAYNPSSAAEELFYDAVDRLNNKGRRAVVVIAGNHDSPDRLCAASPLAYKNG
                     IVLLGYPGSDAGVFGSSSILQSGPGWLEIMPGASEHSAVIITLPYPSESRLEQLISVD
                     SSEEKLQEAYSDKISELLHNLSGNFRNDTVNIIVSHLYLRDGRTSDSERQLGGALVVD
                     PCRLPDNAHFIALGHLHRPQRIKSAPSPTYYSGSPLAYSFSETDYSKLVYIVDAVPGR
                     ESEVKEVYLNSGKPLKQWKAKNGIMEALRWCEEGRDMNAWIDLEVYTDRALTLEEQKL
                     LRELNPGIINIRPVVIDEKESIIDFESREGKKIEELFRDYYRYKTGMEAAEEMMESFL
                     EVINDNQEDDLEEIQPGGEESEAQVS"
     gene            complement(5540..7009)
                     /locus_tag="PKU88_04325"
     CDS             complement(5540..7009)
                     /locus_tag="PKU88_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HPX68542.1"
                     /translation="MYKGVHRLGVEKVKAIIAEIDKKHMIVITDKGDFVKVKRQMSAA
                     IGDEIELKQRRIYPISKHLAGLAACFMACIFLSTGVYAYCTPYSYVSVDINPSLSLAL
                     NRFERVISVNPLTEDAVDLIKDAKSLKNRNIDAALSEIIKTASDKGYINEEAEDEIVV
                     IVSAKNPKQEKKLEETVAKTAEKELSKVNNAGGVTVEKTNVENHKTAISQKVSPGRTV
                     LEESLKKVMPEIKDEVVKDMSVKEVANLIREKRKEAEKAEKAAKEAEKAAEKAAKEAE
                     KEAEKAAREAEKENRKRDESFDNREDREEGDKWDNRNREEDNRENRDDDKDRNNDKNR
                     DKDKDKDDNKEKDDKKKDDKITVPSNSSDKSNNDNNKEPNKDKKRSNDTGNDDRDGSE
                     DEREGGKKNKDDSNGDKKNNSQVRDKDKDNSSSNNDSQKKTNNSSSSNDSQEKVNNSK
                     NSKSKIDNNGKINWNNWIYNFDWLNKNNDSDKNSRSRER"
     gene            complement(6970..7695)
                     /gene="sigI"
                     /locus_tag="PKU88_04330"
     CDS             complement(6970..7695)
                     /gene="sigI"
                     /locus_tag="PKU88_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HPX68543.1"
                     /translation="MFYKSINERVMGIKLNRDERDAFIEEYKPFIAAAVEKTTGRYVS
                     YGYDEELSIGLMAFDEAIIHYDQEKGHFLSFAQGIIRKRLIDYYRKEKKHQAVTYVSE
                     YSTDEADGEEVFEYVIAAPEAQNKYYLEEINQLRRQEIVQLKEELNNWGLEFSDVAKS
                     SPKHTNTKSSYLDIVKYIIDRPELVSKIKEKKYLPIADIEEGLKLPRKTIERSRNYVI
                     AAMIILTGDYYCIREFIDWGWKK"
     gene            complement(7799..11494)
                     /gene="addA"
                     /locus_tag="PKU88_04335"
     CDS             complement(7799..11494)
                     /gene="addA"
                     /locus_tag="PKU88_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HPX68544.1"
                     /translation="MGWTEEQARAISEGRCNLLVSAAAGAGKTAVLVERIIRKITQAE
                     NPVDIDRLLVVTFTNAAAAEMRERIGAALSRVLEEAEGDGNPKVKKNLQRQLTLLGKS
                     NITTIHSFCLDVIRNNFHTIDLDPAFRIADDTEALLLQLEALEELIESKYEPENCTED
                     FLSLVECYGGSKDDNKLMDLVLTIYDFVQSHPWPDIWLRDAIEAFNAAEDTDFGDTKW
                     AEVLMKSISMELAGLQCLLEDTIKAAEREEGLSPYINTLQEDILRIKAMQSACKGKGG
                     WDALYREFDNLGFSRLPKCGKEADPIIKEQAKGARDDVKDRLNRIKKDLICGSGELTS
                     DLRQLYPIMNSLGSLVSEFADKYQDKKKQKNVIDFNDLEHYCLKVLTNRTEGKVVPTQ
                     AALGLRELFEEIYVDEYQDSNLTQEVILGMVSREEAGEPNIFMVGDVKQSIYRFRQAK
                     PELFIKKLRTYSGDSQSKYRKILLNKNFRSREDIIKAVNLVFSRIMSESLGEIEYTEE
                     EKLNAGAVYELPESPGGSAGGPVELHIIDSSEIGAEETDTTEEDFEDYEESALMLNSI
                     QCEARVAANRIKELTADGGFMVMDKNTGEYRPAQFKDIVILMRATKNWAVVFAEELSL
                     QGIPVFTDAGTGYFRTVEISTVLSLLQIIDNPLQDIPLLAVLRSPICGFNPEELIDIR
                     LFEREADFFEAMVKAAASEGELGRKAAGFLEKLEDWREKSLYLSTDELIWYLYNDTGY
                     YSFVGAMPGGAARQANLRMLFEKARQYEESSFKGLFNFINFINKLKGNQGDMGSAKVL
                     GEKDDVVRIMSIHKSKGLEFPVVFLCGAGKGFNLTDTAKNILLHHELGIGPDFIDHKR
                     RIWYPSIFKQALKYKIKLENLSEEMRILYVAFTRAKEKLIITGAVKDIARSTDRWKSS
                     SGANTQKVPEFDVLRGRSFLDWICTALMRDNEGKPAERLESVEGWDIRFWNREEVLRG
                     AVVEEHGKEELIDEAALSSAEANMKDEIIKRLEWKYPYSAYEKLPVKVTVTELKRYFD
                     TEAADEFRMQAAPGIRKRPRFLEETSGMTAAERGSLMHFVLQHMDFGGVVSEAGIKRQ
                     LEDMIRMELLTEVQARNVNINGIIGFLNSSLGKRMIKADRLFREMPFTMEVECSEVFG
                     SLAGCGHEGETVVLQGIIDCWFEEEGKLVLVDYKTDYVPEGEVSRIKEKYRTQIEYYA
                     RALSQITGKEVSGKYIYLFWNGKILEY"
     gene            complement(11481..14882)
                     /gene="addB"
                     /locus_tag="PKU88_04340"
     CDS             complement(11481..14882)
                     /gene="addB"
                     /locus_tag="PKU88_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HPX68545.1"
                     /translation="MSLRIIYGRAGSGKSTYCLNEIRESLNQGNNTRHIIIVPEQFSL
                     QAEKKLIKAVGASGINGAEVLSFRRLSHRVLGEVGGLVRKHINPAGKSILVYTIMEKL
                     RDELRVFAKAAGKQGFVNTLCGTISELKRYNIAPPALGSIETASLSPQLADKLHDIGL
                     IYGEFEKRLHDKYIDSDDDLDILAEKLELYKAFETSEIWLDEFSGFTPQEYGVIAKLM
                     SKAVRVNVLLCTDCLSEDYAPEDTDPFLPARAAAAKLIRLAKDSGIKVDAPVSLKNNG
                     FKFNKELAFLESNFYSYLHGKYKESTEHLKLYEAPGIFAEAESAARDILKKCREEGFR
                     YRDIAVITGDLPQYEKVIRVVFREHGIPCFIDRKKDITGHPLILLVLSALEIFIGNWS
                     YEAVFRYLKTGLTGIDRRESDILENYVLANYVPGSSWMKDEDWEGLSVNEARRRAAAP
                     LLRLYGRIKGKNTVKEICTALFDFLCELEVPEKLEMLVEHFTQRGELDRADEYSQVWN
                     ILLELLDQVVEAIGDEAVKADTFLTILSVGFGEYNIGLIPPALEQVLVGSLERSKSHD
                     VKAIYVLGANDGIFPVPFKQEGILSDRDREILRNMGMELAPDTRGRTFEQQHLIYTAL
                     TASSDYLRLSWCAADREGRAQRPSVIISRLRRMFPEISFESGILRGISGYDADTNVSA
                     PVPTFNELVSAMRQGFEGRYADPLWKSVADWYKKEEQWKEKYFRIAEGLSYTNQAGFI
                     RQERIRKIYGKTIHTSVSRLERYFSCPFSYYVEYGLKAKERPVMKMNAPDMGSFMHRV
                     IDMFSRKVSESDLGWRHLEREWCAGEVSLIVDELVQDAAGKVFLSTNRYKYLAARLKR
                     VLTRAVWLIAEHIKRSAFEPVGYEIGFGEGEEFPAIELELPSGEKMSLSGRIDRLDCM
                     ETEEGKYFRIVDYKSGSRHFKLEDVYYGLQLQLITYLDAVGGSLEEEPDKPVLPAGVL
                     YFRLDDPIIQGSREASEEEIEKAIMKELRMKGLLLADVKVIKEMDTGIDGDSLIIPAR
                     INKGDVLGRSSAATAEQFEIIKRHVRKLLLSLGEEILKGNISISPYRRKKMTACAYCS
                     YMPVCQFDMSISGNNYRNLWEKKDEEIWELMAGKTVEGGEANGLD"
     gene            complement(15230..15988)
                     /locus_tag="PKU88_04345"
     CDS             complement(15230..15988)
                     /locus_tag="PKU88_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPX68546.1"
                     /translation="MVSIGIDIGSVCAKAVAYDERIIGRLIMPTGWNPRQTAKELYER
                     LVSENEIKKGEIASVVATGYGRVSVPFASRKVTEITCHAKGAACLDPDARTVIDVGGQ
                     DSKAISIDEKGNVLEFMMNDKCAAGTGRFLQVMANVLETEVDQLDALASNSTPRDINS
                     MCAVFAESEVISLLSQGTSKEAVASGILHSISNKIIHLASRITIREKVLFTGGVASNS
                     MIREIISKKLKCRVLTHKDSQYAGAIGAAIIGYY"
     gene            complement(16016..16270)
                     /locus_tag="PKU88_04350"
     CDS             complement(16016..16270)
                     /locus_tag="PKU88_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPX68547.1"
                     /translation="MEEECFYVLFPNVTEGLKLEKLLKEKAVKYTIVPTPRELSKSCG
                     ISIMYEFRDDSLIKRIAEEYGIRTEGFKSLTRKKAEVKFI"
     gene            complement(16270..17541)
                     /locus_tag="PKU88_04355"
     CDS             complement(16270..17541)
                     /locus_tag="PKU88_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HPX68548.1"
                     /translation="MADYRELWSNLGMDLEKHDLLCEALPQLFGGVYLIQENRPEGMN
                     YYNFVVSEVHGLRIKELDDYKKAGGKVVGTFCVFVPDELILAAGAIGVGLCGGSQFWI
                     EDGEKVLPRNLCPLVKAIVGAKISGTCPYFQSCDLLTGETTCDGKKKAYEVLGEYAPV
                     YVMDLPQMKRDKDYKKWEEEIYEYKAVLEELTGNKITEEKLGSAIRLCNRKRAALQRL
                     YNARKALNVPISGKDALLVSQIAFYDNSERFIEMTNKLCDELEDRIKKGIGVASEKSP
                     RIMITGTPMAIPNWKMHHLIEESGAVVVCEETCTGTRYFENTVDETQTTMEGMIKALA
                     DRYMNINCACFTPNKGREEDVVRLAKEYGADGVIYCNLQFCHTYNIEYRQVEKALKEA
                     GIPVIFIETDYSNDDTGQLQTRIEAFLEMLK"
     gene            17774..18643
                     /locus_tag="PKU88_04360"
     CDS             17774..18643
                     /locus_tag="PKU88_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPX68549.1"
                     /translation="MNYIIAKLKNDRTRSIIFMIIAALLWSTGGLLLKGLDLNPMAVA
                     GSRSLVAALLMLAIIKKPSLKFNRYKLGFAVAFAATMILYIAGNKLTTAANAIMLQYT
                     APIYVALLGAWFLNERTSRLDWIAIFFTVGGMFLFFLDDLSSGNIIGNILSILSGVSF
                     AFVVMMLRKQKDQSPIELVFWGNLLTAAIGVPFLPGSAPDQSDLVMLVIIGVFQIGLP
                     YVFFAYGTKHVSALEAVLIPVLEPILNPVWVFLLMGEKPGRFALLGGFIVLLAIVGRG
                     VILTLKTKKNPPA"
     gene            complement(18667..18975)
                     /locus_tag="PKU88_04365"
     CDS             complement(18667..18975)
                     /locus_tag="PKU88_04365"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HPX68550.1"
                     /translation="MTTENNLFIILDTTLTKELIEEGYAREFISKVQQMRKNNAFEMM
                     DRINIYYEGDDEIAEAVKAYEEYIMTETLAEKIERVTDANFEVQDLNGHQTGMKVEKI
                     "
     gene            complement(19004..19705)
                     /locus_tag="PKU88_04370"
     CDS             complement(19004..19705)
                     /locus_tag="PKU88_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HPX68551.1"
                     /translation="MKQLPKAVTFDNFGTLIDWEGEIQKYMAKFLERNGIKEVDPKTV
                     QHRWEEIQFDYIQGDYKPYRKVLHDTLAMTFKEFGYAFTEEDCVEFSDSMGTWQPFPD
                     SRDALLELKKYCKIVLITNTDNDIIKESVKQLGVEFDDVITAEMAGAYKPSHKGFDLA
                     RQRLGLKKEEILHAGFGFKYDVVPATELGYETCWINRQGEIRPIDVKETYLVGDMRTF
                     VYMIKGMAQTFAEQK"
     gene            19901..20104
                     /locus_tag="PKU88_04375"
     CDS             19901..20104
                     /locus_tag="PKU88_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPX68552.1"
                     /translation="MDSTKCKVFITSVELGSFSKAAAALGYTISGVSHLVNAFEKEMG
                     FPLLTRDKKGVKPTNNALKIIPV"
     gene            complement(20101..20328)
                     /locus_tag="PKU88_04380"
     CDS             complement(20101..20328)
                     /locus_tag="PKU88_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68553.1"
                     /translation="MKKKLILSLIAPIIILLYQPFGFNTNQSIVYASLLLTITWWVTA
                     AVQKTYASIFLLLTFCIFGSTPLEKIFMFPL"
     gene            complement(20367..21350)
                     /locus_tag="PKU88_04385"
     CDS             complement(20367..21350)
                     /locus_tag="PKU88_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HPX68554.1"
                     /translation="MLILRKSDIEKAISMDEAISAMETAFIKFSEGNTKIPLRTVINV
                     EDKGDAIFMPGYVEGLGVAVKLVSVFPGNLEIGKPTINSVILLEDQNTGEVLALMDGG
                     CITALRTGAASGLATKYLARTDSKSVAVIGAGVQAKTQLWAVCSVRDIQEARIFDLND
                     DRKKRYIEEMSEKFPYIRFAGVSNTDEAVKGADIIITATTSKKPVFSAENVSKGAHIN
                     AMGAYTPEMQELPEKVLKMADKIVVDSREAVLKEAGDLIIPIRKGIFSEKQIYGELGE
                     VASGIIPGRQTDKEITLFKTVGIAAEDVACAAKIYENALLMGLGEEIFLDN"
     gene            complement(21419..21943)
                     /locus_tag="PKU88_04390"
     CDS             complement(21419..21943)
                     /locus_tag="PKU88_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPX68555.1"
                     /translation="MKNEIIFSGVGGQGIMLIGKMLCEAAVDKGYQVTFAPTYGQEKR
                     GGRTSCQIVISDSVDSPVISEADTVLIMDEKSLDDFKDRVKKGAYLIINSSMVEKELT
                     RDDINVIKVPVNDIASEMGNPKSANIVALGVALKYIDSVSLENIKEYLGKIFKGSAIE
                     KNKKALQTGYEYSV"
     gene            complement(21940..22689)
                     /locus_tag="PKU88_04395"
     CDS             complement(21940..22689)
                     /locus_tag="PKU88_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX68556.1"
                     /translation="MKVVYKRPELLTDAHFSYCGGCGHGIINKLLAEIIEEEKLAEKA
                     ILVWPIGCSVYADQNFKVDSICALHGRAPSVGTGLKRALPENTVIVYQGDGDMVSEGM
                     AEIMHAAIRGEKFSVIFVNNAIFGMTGGQMAPTTLLEQKTTTSPYGRRAETSGYPVNM
                     AELISNVRGCYFSERVYVNNPKNIMNAKKAIKKCIQYQMEGKGLSFVEVVSQCPTGWG
                     VNPLDSLKMVETEMSKVYPLGVFKDMGVNDN"
     gene            complement(22702..23769)
                     /gene="vorB"
                     /locus_tag="PKU88_04400"
     CDS             complement(22702..23769)
                     /gene="vorB"
                     /locus_tag="PKU88_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPX68557.1"
                     /translation="MGEKVFMQGNMALAEAAIRAGCKYYFGYPITPASEIMEYYAAKM
                     PEENGVFVQAETEVSASNMIAGVAAAGKRAMMATSGPGISLGAEAISYMCAAQLPAVL
                     VNVMRPGPGDGDIVACQGDYFQAVKGAGHGDYNTIVLAPYSAQEMVDFAFKAFELAEK
                     YRNPVMILSDGGLAKMMESVELPDRIAPDTQFPWALTGAKNRDANVITTCAKSPKQWE
                     EYNLVLQEKFIKIKDAEQKWESYKTEDADVVVVAFGISARIAFSAVKMARDNNMKVGM
                     IRPITLWPFPEKAFKGLDDKYFFVTELNSGQMVEDVKLSVKDKEKVSFYGRLGGMTPT
                     PKELYEKLIELYKEHKLNANI"
     gene            complement(23772..23999)
                     /locus_tag="PKU88_04405"
     CDS             complement(23772..23999)
                     /locus_tag="PKU88_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX68558.1"
                     /translation="MNLVKIDMEKCKECGYCINFCPKKVVLEQGDSINKKGYYPAAVI
                     NQKECTACGTCAKVCPEAAIEVIKDFEGQEV"
     gene            complement(24078..24500)
                     /locus_tag="PKU88_04410"
     CDS             complement(24078..24500)
                     /locus_tag="PKU88_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HPX68559.1"
                     /translation="MVGKKVLLRARMSSGDAHYAGELVNGSKILDYFGDLGTELCIRY
                     DGDESLFLSYNSVEFLAPVYAGDFMEYYGWIEKAGNSSRVCKFEAYKVIELARDPNLA
                     LSAANVLAEPLLVGKAEGILVVKKGLQRGAQDPDFKHE"
     gene            complement(24531..25301)
                     /locus_tag="PKU88_04415"
     CDS             complement(24531..25301)
                     /locus_tag="PKU88_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPX68560.1"
                     /translation="MEKKPYYLIQSVVRTLQIIEYLAETGESSVKEVAEFIGESKTTA
                     HRFLASIKQEGFLEQDSESKKYKLSLKLFEIGNKIVDTLDLRVIARPILQELSLLSLQ
                     TINLGILNKSDIVYIDKAISGNNLRLDSPIGGRDPAHCTAMGKSILAFLSKSIQESYV
                     SEYGLSMRTANTITNPTDLYIELDLVKQKGYAMDREELALGIECVAAPVFDSFMHPIA
                     AISISMPNNPENMGRMEEYQEQILIASKKISSKLGAKI"
     gene            complement(25311..26276)
                     /locus_tag="PKU88_04420"
     CDS             complement(25311..26276)
                     /locus_tag="PKU88_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HPX68561.1"
                     /translation="MSLNVVITDCSWGNIEVEKEYLPENAVVEGYQLITEDEVIEACS
                     DADAILSEYAPITAKALKALKKCKIISNSAIGFDNIDVKAAEELGIAVANVPGYCAHE
                     VADHAMSLILAASRNIVVYDKAVRKGEWNYEIGLEMDRLKGQVLGLAGFGKIAQFVAK
                     RAQGFGFKVIANDPFIKQEYADSLNVKLVEIDKLLAESDIISCHIPAIKDTIGYFNKE
                     KFDKMAKCPIFINTSRGRLVNEEDLADALENKKIRAAALDVLIEEPPTMNNRLFTFDN
                     VIITPHAGFFSKTALREVRSRSAMNVTNFFQKEFNKINFVNKIRG"
     gene            complement(26464..29562)
                     /gene="ileS"
                     /locus_tag="PKU88_04425"
     CDS             complement(26464..29562)
                     /gene="ileS"
                     /locus_tag="PKU88_04425"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPX68562.1"
                     /translation="MDKFKFLSEGRIEENEKRISEYWNEIDILKKCVETREGKEPFIF
                     YEGPPTANGKPGLHHVISRTLKDTVCRYKTMKGYQVKRKGGWDTHGLPVEIEIEKELK
                     LTGKQDIEKYGIKEFNQKCRESVFKYVGMWRDMTEKMAFLIDMDNPYVTYENEYIESA
                     WWILKKFKDEGYLYEGHKISPYCSRCGTGLATHEVAQGYKEIKTTTVIVKFKKKDANE
                     YFLAWTTTPWTLPSNVALTVNPKETYLRVRQNDEVYYVEKTLAAKVLGEDFEVLEELR
                     GTEMEYMEYEQLIPFVKADKKAFYVTVADYVTTEDGTGIVHTAPAFGEDDYNTGRKYD
                     LPVLQPVSMEGKFIGTPWEGKFVMDADLDIIKWLAAEGKLFKKEKVDHNYPHCWRCGT
                     PLLYYAKPSWYIEMTKLRDNLVAYNNAVNWFPPYVGEKRFGNWLENVNDWALSRDRYW
                     GTPLNIWRCDCGHTDSIGSIKELVDRAIEDIDENIDLHRPYVDEVHIKCEKCGGTMTR
                     VKEVIDCWFDSGAMPFAQHHYPFENKENFHELFPANFISEGIDQTRGWFYSLIAISTF
                     VMKTAPYRNVLVGGHLVDKDGKKMSKSLGNAVDVFDMFEKYGADALRWYMLYVSPVWT
                     TTKFDEDGVKDVLSKYFGTLKNVYTFFALYANTDSIDPKDFFVEYKDRPELDRWILSK
                     YNNLAAEVNAALDVYDLTRAARKLHTFVNEDLSNWYIRRSRRRFWDAELTEDKKAVYN
                     TTYEILVGVSKLMAPLAPYLPEEIYRNLTGELSVHVAYFPEVNEDLIDNKLEERMDLV
                     RSLVGLGRASREQVRIKVRQPLQKVFIDAKYEELISYLIPLIQEELNVKEVIFEKNLN
                     KFIDFTLKPDYKFAGPVLGAKIKAFAKMLEEMDAAVAAPKFEAGETVKIELEGEPLEV
                     NKDYVITRIAAKEGFTMTTENNLFIILDTTLTKELIEEGYAREFISKVQQMRKNNAFE
                     MMDRINIYYEGDDEIAEAVKAYEEYIMTETLAEKIERVTDANFEVQDLNGHQTGMKVE
                     RI"
     misc_binding    complement(29626..29848)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(29975..31162)
                     /locus_tag="PKU88_04430"
     CDS             complement(29975..31162)
                     /locus_tag="PKU88_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPX68563.1"
                     /translation="MLTDILIKSFIKDKDNIRDKSVRQKYGYLGGFVGIACNVMLSGA
                     KIAIGLMINSIAITADAVNNLSDAASSIIMVIGFKITSKPADREHPFGHGRIEYISAM
                     VVSFMVILAGFEFFKGSFDRIRNPETVKFEIIPFSVLLLSIGVKVWLSRFYKKISREI
                     DSKAMEASAADSLSDVTATSVVALSLLASLWTTFPLDGYVGLLVSALIIYSGISLTKD
                     TLNPLLGEAPEPKFIRSITEKVLSYDGIIGIHDMIVHNYGPGRCVVSLHAEIPASMDI
                     MKAHDVIDLAEQEISEEMDIHMVIHMDPINTDDEVVQKTQEQITGLINEMHEELTIHD
                     FRIVGGEEHKNLIFDLVVPFEYDEKMAGDLSKKVAWEIKSKYPECNAIIYIDRAYSVL
                     DKL"
     gene            complement(31409..32545)
                     /locus_tag="PKU88_04435"
     CDS             complement(31409..32545)
                     /locus_tag="PKU88_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPX68564.1"
                     /translation="MKKRHRYILLSSVIIINALFLAGCWDERELDELVIPIIIGYDYA
                     NEEEKEQPDDKYLISAGFPIFYEDTLQKYHVDITSGSMEGEVVNRRNTHFGEQLIPGQ
                     LQVVVLGEELISNENIIELTDILSRNPAVKGAIPMAVSKGKAADMLSVPVKQYPNPGI
                     YIQKLIKNIRRTNFFPTCTLFESNRHIISNHTAPLLPYIVHENGNIVFAGSCLLNGGK
                     RIVHLDREKTETAVMLRGIRSRGMITYDIIENNKVIGQITFEGKNKRKVKVKKEGDRY
                     IFNILIKLSGRLYERKKNEPVIDHKDFIKLCQKSLEDLIRKRAEDFVTIVQEEYGFDA
                     LELGIEIKGHSREKISKEDIDRIIQESEISVEVKVCIDNAGGKI"
     gene            complement(32529..33635)
                     /locus_tag="PKU88_04440"
     CDS             complement(32529..33635)
                     /locus_tag="PKU88_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPX68565.1"
                     /translation="MKTEMSYTITSKQLLFTLISCMLGAGILSLPRVTAEAVGQDAWL
                     AVILGGIIPLVSIWLSNKVVSRHPGLSFAALTEKLAGKWAGKLLAAVFVTYSVIASAA
                     ILRVFTEVITIFLLPNTPILVKILLGLAVSAYMASGGIKVLGRLNEFLFYVLLPPLFL
                     SIPAVVKYADLHYLMPVLNNNLAEYSKAAFTTGFAYSGFEAIIVFAPYVLKKKETLKA
                     AVLALIITIMIYLYAVITTVTVFGPELTQMFTWPTLRLLSVTEIPVLERIEFIAIIAW
                     IGVAIRPVSNQYFCAGLIIKDAFGIKSLSKVVALLYPIIAAIAWYPKNIGIVFRLSDF
                     VGMYSLILGTALPLLLLGLSLIRGKGRARDEKKT"
     gene            complement(33625..35217)
                     /locus_tag="PKU88_04445"
     CDS             complement(33625..35217)
                     /locus_tag="PKU88_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPX68566.1"
                     /translation="MKKYRIAPKAVKPMSTIKFRTKASAGGNNADSSDFFDRTADRLR
                     KDLSGNADVIMRELFIGGNENIDALLVYLMGNIDKKLLSDSVIKPLMTCGGLGDIEAR
                     GERLKEHITKKCLQTGSVKYSKDYNTALQDMFSAEGILLVRGIEGFIIFTVAGGQKRQ
                     ISEPETEKRIRGTKEGFIEDIWVNIGMIREFIKDPMLKIEMLKIGQRSQTDVAIIYIN
                     GLADSKIVDEVKKRINKINIDAILSSGYVEQYIEDNPWSVFPQLLGTEKPDKAVANLL
                     EGKLLIITDRSPFVFTAPAVFIEFFQATEDYYEKFHAGIFSRIIRLIAFLIAVLGTSI
                     YIALTSFHPELIPSELLISFARLRKKVPFPPVIETLLMEFTVEILREAGIRLPTSVAG
                     TLGIVGGIILGDAAIRSNLVSPAMIVVIAITTVCNFVMPNYSMTLSMRLLKLFLILLS
                     AVFGAYGIALGLLVIVTHLARLESFSVPYLSPYAPFRFSDMKDALLRLPLWMHVKRPV
                     SIPHMDDKRAVNNREKSGTGEN"
     gene            complement(35441..35818)
                     /locus_tag="PKU88_04450"
     CDS             complement(35441..35818)
                     /locus_tag="PKU88_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPX68567.1"
                     /translation="MDITVSNSTKEPIYEQVANQIKKQIINQQLKPGEALPSIRKLAT
                     DLHISVITTKRAYDELEKEGFIETVIGKGCFVSRENKEFIREKKIRMVEEKLSDVIKD
                     SRVLNIGLQGLKMMLEVLYREGI"
     gene            36128..37480
                     /locus_tag="PKU88_04455"
     CDS             36128..37480
                     /locus_tag="PKU88_04455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPX68568.1"
                     /translation="MRSVKTLVYTALILGIALYLRLRFIIYAPFEFKIDAWSMYNYAL
                     NVMNGEYYGHSFGEHWSRYAYWPPLYIFLSGFVYRFFGTAQDFIVMRVIQAGFSAVSC
                     LLCFITAREALRDYSDLHEEAGLTAGILMALNTRMVAYTNHLYAETVFITIYLAAVYF
                     SLRYFKDEMSLKLSCPNGSVRVKHLVLFSIFFGLGNLTRPVLLLLPAVIFLSALLFIT
                     ARRFSICRGIKVLAADGALSLLIILAVLSPWILRNYAVTGRLILIDTNGPINFYIAHN
                     PHSNGTWVDIKKAQSDMLKLYETGYKEGLVYIRNNINREIDLLKVKQQLFLYHGDYHI
                     SEAATHLDRTYKLPLYGSLIKMECPYRLPQISFEFLWKSALLLLVLFIFRLISVKSLF
                     ILFCEPLWIIGNFLYMNLIILIFYYAPRYRITAEPFLCILIGILLAGIFTSYRKNNAI
                     "
     gene            complement(38048..38935)
                     /locus_tag="PKU88_04460"
     CDS             complement(38048..38935)
                     /locus_tag="PKU88_04460"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HPX68569.1"
                     /translation="MEGVKRLDGWAKFKGFIHLLRPKQWIKNLFLFAALLFSRNMDKP
                     QYVMTVLYAFLCFCMISSAVYVFNDISDAEKDRQHPRKRMRPIAAGIIDRREAQILMI
                     ILLPVSVILSIMLDYSFGFIVMTYLLNNVVYTLYVKNIVILDVMSIALGFILRVSGGA
                     VVIGVTISPWLLLCTFLLSLFLGFSKRRNELLMLQADAHNHRNNLEHYSLDFIDNMLS
                     IVTASTLISYSLYTFYASEGKHSMVTILFVLYGIFRYQYIVYNKKMGESPEEIILTDI
                     PLIVDIMLWILVSIIILYI"
     gene            complement(39094..40113)
                     /locus_tag="PKU88_04465"
     CDS             complement(39094..40113)
                     /locus_tag="PKU88_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPX68570.1"
                     /translation="MNYNEVLDKAKSQIGSKCRVCRECNGVVCRGEIPGVGGKGSGSG
                     FIRSYEKLNEIKLNMDTIYESKEVDTSIELFGKSFAYPVFAAPIGGLQQHYREDSNDY
                     EYSHAIVSGCKNAGTAGFTGDGVKDEFYEQPLKAISEAGGWGIPTLKPWKNDEILRKI
                     KMAEAAGALAAAMDIDAAGLALLAALGKPVSPKPVEEIREIVAGTRLPFIIKGIMTAA
                     GAAKAVEAGAYGIVVSNHGGRVLDHTPAPVEVLPEIYKAVGGKIKIFIDGGIRTGIDI
                     VRVLALGADAVLIGRPYAIAAYGGGAEGVELYTRKIGAELKDAMIMTGCSTLKDIDER
                     VIYKP"
     gene            complement(40145..41173)
                     /locus_tag="PKU88_04470"
     CDS             complement(40145..41173)
                     /locus_tag="PKU88_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68571.1"
                     /translation="MKRTGLTTIWAAILASCLLIFAFAEDTGKIVQGTESDARIIYNG
                     KEAVLGLKPVLINGSNYLSVRAVSSLFEKNIYWDSSKNEIIITDKTNPQLEYLSSELA
                     LKDKTIQELEVKLKKLESKAESAERLSIEELQEEINNEFGNYEGVSYRVILSGNEDEV
                     RTIFEIDLSRDRLSWNSLSAKEKYELVEEVAEVIMEEYASAKIKGYYKDISDSRILFS
                     FYYNWEGELVKGNYENYSAISILEEEFNNEYADYLKGIHFTFTLKGNDNITEFTAYIQ
                     RDRFRKEWNSLSDKSLKNFMEKLCSDIAGEFEECYIYGYIYDTDSNEELAYCEQTVEG
                     DFVFGRED"
     gene            complement(41189..41902)
                     /locus_tag="PKU88_04475"
     CDS             complement(41189..41902)
                     /locus_tag="PKU88_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPX68572.1"
                     /translation="MGVFINSERKEISMKYGDLRNKQGITEKEFLARYDAGRYDRPSV
                     TVDMLVFSITGEMKKELKILMVKRGNHPFLGYWALPGGFVNMDESLDAAALRELKEET
                     GIEDICMEQLYTWGDVDRDPRTRVIGCSYMAIVDSSKLAVKAGDDAEDAAWFKVECSS
                     IDQKKPINNVRLVLSNDRDTLEAIIKVDRAFVGNTARVSRVISESRGIAFDHGKIIEY
                     AVESMRNRLEYRDIGLLTE"
     gene            complement(41938..42444)
                     /locus_tag="PKU88_04480"
     CDS             complement(41938..42444)
                     /locus_tag="PKU88_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPX68573.1"
                     /translation="MSLFENWRDTAYNNEQDEKAKKKFWEGYFEKEESIYEKLLSNPE
                     EVVEGTVAELAERYGTDILLFTGFLDGINDSLVNPMELEALEESSSIKLEIDFEKLYY
                     NMLKAKADWLYNLPQWDNILDDGKRKEITKKQRLSGTVIKSKEPGRNDPCPCGSGKKY
                     KKCCGANK"
     gene            complement(42597..42977)
                     /locus_tag="PKU88_04485"
     CDS             complement(42597..42977)
                     /locus_tag="PKU88_04485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPX68574.1"
                     /translation="MKSKVTDASKPANKRKIRRIDYYSKIQCIKSTCEGRTEEYEIPL
                     ELTLINISVGGLGIISEKPFEKDAVLILSFKLEEVKYEKVSAKVMWMMKMGDMYRHGL
                     EIMNVSGKLFKHLSMLDNSITTKV"
     misc_binding    43124..43389
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            43440..44429
                     /gene="asnA"
                     /locus_tag="PKU88_04490"
     CDS             43440..44429
                     /gene="asnA"
                     /locus_tag="PKU88_04490"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPX68575.1"
                     /translation="MSEMYSLSIRETEVAIKVIKDFFEAQLAKKLNLTRVSAPLFVER
                     ATGLNDNLNGIERPVSFDALSLKGSELEIVQSLAKWKRLALSRYGFLPGEGLYTDMNA
                     IRRDEELDNLHSIYVDQWDWEKIILREERTPETLKAVAKTIFSVFKDTEELIFKRYPQ
                     FKPALPEEIFFITAQELEDMYPDKSPKERENLITKDKGAVFLMQIGARLKSGTRHDGR
                     APDYDDWSLNGDILFWYPVLQCAVELSSMGIRVDEEALLRQLEISGCMCRKELPYHSS
                     LLKGELPYTIGGGIGQSRICMYFLQKVHIGEVQSSIWPGSMVSQCEKKNIKLL"
     gene            complement(44426..>45063)
                     /locus_tag="PKU88_04495"
     CDS             complement(44426..>45063)
                     /locus_tag="PKU88_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX68576.1"
                     /translation="QQAGLAYTTAGKAAFITGLYIVLVPLLGIFLKQRVGLNTWLGVV
                     LAVSGLYFLSVNEDFTIAFGDLLEIIGAFFWAVHILVIDYFTKKVDALRLSCAQFAMC
                     SALSIIIALIFEDISVSGLSQAAIPILYGGLLSVGVAYTLQVVAQKHAKPSHAAIILS
                     MEAVFAALGGALLLKENMGVRGYLGCALMFAGMLLTQVNFFEKKESAEYKA"
     assembly_gap    45064..45163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45164..45489)
                     /locus_tag="PKU88_04500"
     CDS             complement(<45164..45489)
                     /locus_tag="PKU88_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX68577.1"
                     /translation="MNNREMKSNMLLMLTAAIWGFAFVAQRVGMQYVGAFTFNGVRFA
                     LGSISLIPLIIYFKNKKTAEQPEETASASALVPGIIAGSVLFFGASLQQAGLAYTTAG
                     KAAFITG"
     gene            complement(45577..47952)
                     /locus_tag="PKU88_04505"
     CDS             complement(45577..47952)
                     /locus_tag="PKU88_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPX68578.1"
                     /translation="MGSIFESKELLIFDGGMGTMLQAHGLKAGELPESWNISNPEKIL
                     EIHKAYIDAGADIITTNTFGANRYKLKGSGCSCGEIIGSAVKIAKEAAGSKLTAHDIG
                     PIGQLMEPYGTLSFRDAYDAFREQVVAGAAAGADMILIETMSDIYEARAAILAAKENS
                     HLPVLCTMSFQLDGRTLTGTDPLTMVNIISHLGVDALGINCSLGPKEIIPLLARVLKH
                     STVPVIVQPNAGLPIILDGKTFYDISPEQFGQYMAETAKMGAAILGGCCGTTPEHIKE
                     LRKAVSGLKPVRPEAVKLTAASSATATVILGEGAKIIGERINPTGKKRLKEALRNGDM
                     DYLLKEAVAQRDAGAHILDVNVGLPEIDEKAMMVRAVFELQNIVNLPLQIDSVSPDVI
                     EAAARICSGRPIINSVNGEKKTMDTIFPIAKKYGCLVVALTLDENGIPEKAEDRLKIA
                     EKIVNRAEEFGIGREDIIVDCLVLTASAQQKEIRETIKAVALVKEKLGLKTTLGVSNV
                     SFGLPDRELLNRTFLAMALTAGLDAPILNPMAEAMTDTIKAYNVLWNIDKEAAEYISS
                     RSEVNEQPVFKEKEENRSLKKLIVDGMKEEAALTVKKLLTEINGMEIVDTFLIPALDV
                     AGQKYESGEIFLPQLIQSAETVKRAFEAIKDNMLKNAGEVTGVSKGKIVIATVKGDIH
                     DIGKNIVKILLENYGFEVYDLGKDVAAESIVKKVKEEKALMVGLSALMTTTVKSMEDT
                     VKALRQHCPDTRVMVGGAVLNEDYAKMIGADYYGKDAREAVKIARRFYGCE"
     gene            complement(47952..48605)
                     /locus_tag="PKU88_04510"
     CDS             complement(47952..48605)
                     /locus_tag="PKU88_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPX68579.1"
                     /translation="MYIDRKEVLRYMGYRGQNIGHTTEKLLNDCIDEVASVSKESFLY
                     EIFDVEHTAEGLCLKGTTLTLAGEDIGKHLANSEKCALMAVTLGLEVDKRITFYSKTD
                     IAKALVFDACATAAAESLCDTVHEKIETEARSMGFETTTRFSPGYGDFSIGIQKELAK
                     VLKAYEGIGLSVNESSIMIPRKSVTAFIGLQREKSDKSGHSCESCDSKNCFFRKGVE"
     misc_binding    48747..48996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            49072..50235
                     /locus_tag="PKU88_04515"
     CDS             49072..50235
                     /locus_tag="PKU88_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPX68580.1"
                     /translation="MTVKKTFEEINEKIRKGNAVVVTAEELADIVEEKGISDATKYVD
                     VVTTATFGPMCSTGAFLNFGHSDPPIRMEKIELDNVSAYGGLAAVDAYIGAAEPSTDR
                     GIEYGGAHVICNLIDGKKVHLKAYSKGTDCYPRKEIDTYITKDSINEAYLYNPRNCYQ
                     NYSAAANTSDRTLYTYMGVLQPGLGNVNYSTSGELSPLLKDPYLRTIGIGTRIFLAGA
                     EGYVAWQGTQFASGKPRGENGIPLSGACTLAVIGNLKEMSTEYIQPAIFEKYGVSMFV
                     GIGIPIPVLDSDLLFNLSVQNKDIYTNILDYSVQRSNKPVLAQCSYEELRSGFVSIEG
                     KKVKTSPLTSLYKSREIARVLKSWVAEKGFTLQPPVAPFPKGVTLKSLEQGRL"
     gene            50237..50455
                     /locus_tag="PKU88_04520"
     CDS             50237..50455
                     /locus_tag="PKU88_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPX68581.1"
                     /translation="MQGCIHCGACTAACTKGALKMESESGTVSCDESLCTHCNACIRA
                     CPARALTYNQIRRPYVSEKRLSEAYEGR"
     gene            50412..51134
                     /locus_tag="PKU88_04525"
     CDS             50412..51134
                     /locus_tag="PKU88_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPX68582.1"
                     /translation="MYQKRDYRKLMKADDLICFNVTEYESDLLIYARTNLKKEAKIAV
                     AKYRKDIIDYIRRHPEFLTSLVPINPILDAPEIIQDMCHCSKLANVGPMAAVAGAVSK
                     YAGRDLLRFADEVLIENGGDLFIKSKNDRRILIYAGNSPLSNKVALLIPGNNQELGIC
                     TSSGTVGHSLSFGKADAAVVISKDAVLADAAATAVGNVVKDKNHIEAGIEYAMSIPGV
                     EGVVIIADDKMGACGDINITKP"
     gene            51149..>51924
                     /locus_tag="PKU88_04530"
     CDS             51149..>51924
                     /locus_tag="PKU88_04530"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPX68583.1"
                     /translation="MKIKEFFKNKPVISLEIFPPKPEMPIDTIFNTIDALSGLKPAFM
                     SVTYGAGGSSKAHTVKIADVIKNKYNIEALAHLTCIGAKRDEIDNILKQLKDYNIENI
                     MALRGDIPVGSDPASLENAEYKYAKDLISYIKEKDSFCIGAACYPEGHIECPDKVQDL
                     KHLKLKADSGADFLITQLFFDNNLYYSLLEKLDILDVKLPVSAGIMPVINKRQIERIT
                     ALCGASIPPKIKRILDKYGDNPEALKEAGIAYATEQIIDLL"
BASE COUNT        12965 a        13044 c         9439 g        16376 t
ORIGIN      
        1 ccatcggtaa ctatataggt tgatattatc aagcctgctg ataagataag ggccatcaaa
       61 ataagggcaa tgcttttcct gttttcctga ttcattattt aacccccttt aatatatttt
      121 aggtactata cacaaaagca gaagaatgct atatatctga agtttgcata atcctccgca
      181 agagactgta tagtatatcg acgcacacgg attatgattt tgacaggcct actgtgaagc
      241 aaaacaaatt aatacgaatc cgttttgctt agcctttgga aaagttctat cggtgtcaaa
      301 aggccctcca tggcctgaga cacctaagcc ggcgtccatg ccggcttcac gagcttttta
      361 ccaaagccat atcagaggcc tgttgccaaa atgcataaaa gtgtgccctg ctatactata
      421 caagctcttg cttggttatg caccctatag aacactgact tccttataaa ccttcaaatc
      481 cctgctctaa tctcgtgtct cgtgtctgaa gaaaattgcc acgcaatttt cttatgcctt
      541 ttccataata accctgcttc ccgagccgtt gaccatcgga gcctccacta caaggcgcct
      601 tgttatcctg cttttcaatt caggtacatg gcttatcagc cctatgaccc tttccctggt
      661 gcttaacctt tccagagcat ctatgactgt atccagaagg ctgctgtcca gggtgccgaa
      721 gccctcgtca aggaaaaaga actcaagggg gctctgccct ttaagctgta tttgagagga
      781 taatgcaagg gcaagggcta aagacgtcag aaatgtctct cctcccgaca gggttgtaac
      841 cggccttcga atacctccgt tggcatcgtc ccggatgaca aaaccgtttt cggtatccag
      901 ctccagcccg tatttgtgcc tggtcaaaat acccagggtt tcagaggctt ccctggcaat
      961 gtatctgagc cgttcttccg atatgtattc tatgaaactg ttccctttaa gcaggctctt
     1021 tataagttca agatgctcct ttttctttga gtactccttc agctcctttt tcagcaaaag
     1081 ccagttctca tagttggatt ttatagtttc aagcctgttt ttttcactct cgtaccttgc
     1141 aatgctattc tctttttcaa gctttagctc ctcatacctg cggtttattt cctgccacag
     1201 ctcttctgtt atgcttcgcc cggacagctt cttttctatc agtgccttat gtgctgttat
     1261 actgctttcg gccttttcat actctttaat tatttttacc ctttcctcca gctcttcctt
     1321 tgtcataaag gcttcctcca cttcctccaa agaagaaaaa cctttatcgg caagctcctt
     1381 atccagtttc tttctgtcat ctgacagttt attccggtat atctcacttt gcttctcaag
     1441 aaccttctta tccttgactt ccttttcgta cctttctctc aatgtattga cgttttgctg
     1501 ggctttttgt tccccttttg ttaaaaaaga aatctcatcc tccgtcttta atatctcatt
     1561 ctcaatatcc ttatccccta taaagtcatg aatctctttt tccttctctt ccttctgata
     1621 tttgaagccc cttccttcag ccgtgttttc ggaaagcttt tccatatcaa gcttccttgc
     1681 ttcttccgcc ttatcaatct cttttctcag ctctgaaata ttttgttcaa ttatttgcag
     1741 ctcctcatat gtattttctg cttctttgtc cttctcctgt atcctccgaa gctcatccct
     1801 catgcttcca atccccgtat ctttcactgc ggcagtatag gtcctttctt tttcggccca
     1861 tgcttctttt gcctcagtat aggccttctc cagatcctta aggtgcttac tgtctgcttc
     1921 aaggcgggat gccatgctgc tgttttccac ctggtaccgg ttatattctt ccgctatctt
     1981 cttatggctt tcttcaagct ctgccagctt tttctcccat aattcaatgt ctttcagctt
     2041 ttcctgtttc tcttcaccgt ttttacccaa ggccctctct atttccggga cggttaatcc
     2101 tttataatcc tcaggcagcc tttctataag ctcagcctgc tcttcctgct ttttcatgat
     2161 atccgacaat aactcttcct gctgcttctt caagttatta cgctgctccc caagcttaat
     2221 gcccttgttt tccatggccc ttaattgcgt ctcaagcttg cggattacgt cctgaagctc
     2281 ctttaccgct tcagcttctg ttccatggcc gttttttcga tgggcagcag gattgggatg
     2341 ggttacagct ccgcatactg gacagggttc attttcctta agattctgag ccagcaggaa
     2401 tgctgcgtgg ccttcctgct cctttttcct ttccccaagg agcagcctaa gactttcaat
     2461 ctctgaaagc tcctcaagct gagcatccag ggcttcaaat tgtttgccgt tctcaagctc
     2521 tttctgcctc aattgttcca tatccttgta cttaagctta agtgcatcaa caatagacct
     2581 gatactatag tattttgtct cttctttatg tatgtcattc ctgtcccact ttttgctttt
     2641 cttgtgctgt tcataggagc tttgaattgc ttctttctct ttgagaagct tcaatcctgt
     2701 ttctttaaag gattcaatct cctgctcctg caggattata atgtctgcca tctcttcata
     2761 tttggctttc tgtttctcct tttccttctg cagccgctcc aaatcttctt cgaggcttgc
     2821 cccataccgg attttatccc tgcagcccgc ttcaacctta atggcttcag tgcgggcttt
     2881 ctttccggct gcttccttca ggagcccttc aagcccggac ttctgttttg ctatggtttc
     2941 gttccaatca tcaacttttt tcttcagaat tacatgttca ttcctaagct ttctaagaac
     3001 ttcctctatt ttttccagtt cactttttgt cttaaggacc ttttcaagct tagtcctatg
     3061 ccctatgaga aggggtatct tctcctgtct tgccttagtt gcagattcaa gctctgcctg
     3121 tccttgctca agctgtcctt ccaattcgca aaggcttgta tttagcctct ccagctcttt
     3181 tgtcacattc tccaattccc cgccggtttt cttaatatca ttgatcctat ccataaggct
     3241 ccgtgctgct tctgctccct tgcagctgat cctcattaag tctatttcag ccctgcctgc
     3301 aagcagttcc tgatattttt cctctgtctc acgatattcc gcagaaagct cccagacctc
     3361 tttcgcaccg taatattcat cttccgccgc cctcaaggct tcagccacgc tcttcctatg
     3421 cttttccgcc gcaattaaac cggcttccga atcctttaat gaagcatctg aaatatcccc
     3481 caatacggat agtgccctct caactcccga aagcctgtta cggattcttc ccagccttct
     3541 gttgagcttt tccgtaagtt cccgtccgta ctcttcaagg tagaatatgc gttccagcat
     3601 ttttgtcttg tcacccttgg cagataaaag gaattcctga aatttattct ggggaagcac
     3661 taccgaacgt gtaaaatcgt caaactgcag ccctatgagt tctattacag cctcattcac
     3721 ttctcccggc ttgtcggcaa taaccgcatc ccccgaggga ttcagctcta tcagcctggc
     3781 tgttcttgat tctatggagt tctcagagtc cttcttccgc ctgtactccc tttccacctt
     3841 gtatgacttt ctcttcttgt ctttcaccag atcaaaggta aatgaaaccc gcacattggc
     3901 tgaagacaag tttaatatac cctgggtgcc tctgtttgct ctctggacat ttccgtacaa
     3961 agccaaggtt atagcatcca gtatggttga ctttccgctg cctgtagggc cgaatatccc
     4021 aaaaaggcct gtctccccaa gcctgtcaaa atctattgac tgaagctcct taaagctctg
     4081 cagcccctct aattcaagat acctgggcct cactttcctc accccccggc tgtatttctt
     4141 ccaagtcatc ttcctgatta tcatttataa cttcaaggaa ggattccatc atttcttcgg
     4201 cggcttccat tcctgtcttg tatctgtaat aatccctgaa cagctcctct atctttttcc
     4261 cttccctgct ttcaaaatct attatgcttt ccttctcatc gattaccacc ggccttatgt
     4321 ttataatccc ggggttaagc tccctaagaa gcttttgttc ctccaaggtc agagccctgt
     4381 cggtatatac ctccaagtct atccacgcgt tcatatccct tccttcctcg caccatctca
     4441 gcgcttccat tattccgttc ttagccttcc actgcttcaa cggctttccg gaattcaggt
     4501 aaacctcctt tacctcagac tctcttccgg gtactgcatc cactatatat accaatttgc
     4561 tgtagtccgt ctccgaaaaa ctgtatgcaa gaggtgaacc ggagtaatat gtcggtgaag
     4621 gggcgctttt tatccgctga ggcctgtgaa ggtgccccaa agcaataaaa tgagcattat
     4681 caggaagccg gcaaggatct acaaccaaag cgcctccaag ctgcctttcg gagtctgaag
     4741 tccttccgtc cctaagatac aaatgactta ctatgatgtt cactgtatca ttcctgaagt
     4801 tgccgctcaa attatgcaaa agctctgata tcttgtcgga ataagcctcc tgaagctttt
     4861 cttcactgga atcaaccgat atgagctgct caagcctgga ttccgaagga tagggcaggg
     4921 tgattatcac agcgctgtgc tcggaagcac cgggcattat ctcaagccat ccggggcctg
     4981 actgcagtat ggaactactt ccaaacaccc ctgcatcgct tccaggatat ccaagcagta
     5041 caataccgtt cttatatgcc aaagggctgg cggcgcacag ccggtcaggg ctgtcatggt
     5101 ttcccgcaat tacgacaaca gcccgcctgc ccttattgtt cagcctgtca accgcatcgt
     5161 aaaacaattc ctcagcggcg gaagacggat tatatgcatc aaaaatatcc ccggctataa
     5221 gcaccaggtc tgccgattca ctttctgcta ttccgcaaag ctcgtctatg aactgccttt
     5281 gctcgtctaa tctgttaata ttgtcgagag tcttgcctaa gtgccagtct gaagtatgaa
     5341 gtatgcgcac ggccattccc ctccttgtgc ttgacttatt ccattatctt tattctatcg
     5401 aattatttgc caaaatacaa tttaatatgt ggttaacata acaataggac acgggaagga
     5461 cacggggacg tttctcctgt ctccctgaaa gggagacagg agaaacgtcc ccgtgtcctt
     5521 cccgtgtcct cgttgctttt tatctttctc tgcttcttga atttttatcg ctgtcgttgt
     5581 ttttatttaa ccaatcgaaa ttgtaaatcc agttattcca attaatctta ccgttgttgt
     5641 caatcttgct tttactgttc ttgctgttat ttaccttctc ttgagaatca ttgctactgc
     5701 tgctgttatt cgttttcttt tgagaatcat tgttactgct gctgttatct ttatctttgt
     5761 ctctcacctg gctattattc ttcttatctc cgttactatc atccttattc ttttttccgc
     5821 cttctctttc gtcttcgctg ccatccctat catcatttcc cgtatcattg cttcttttct
     5881 tgtctttatt cggttccttg ttattgtcat tattgctttt atctgatgaa tttgaaggta
     5941 cggttatctt atcatctttc tttttatcgt ctttttcctt gttatcatct ttgtctttat
     6001 ctttgtcccg gttcttatcg ttgttccgat ccttgtcatc gtcccggttt tccctgttat
     6061 cttcttcccg atttctattg tcccacttgt cgccttcttc cctgtcttca cggttatcga
     6121 agctttcgtc ccttttcctg ttctcttttt cagcttccct ggctgctttt tcagcttctt
     6181 tttcagcttc cttcgcggct ttctctgctg ccttttctgc ttcttttgca gccttttcgg
     6241 ctttctccgc ttcttttctt ttttctctta taagatttgc aacctctttt accgacatgt
     6301 cctttactac ttcatccttg atttccggca ttaccttttt caggctttct tccagcaccg
     6361 ttctccccgg tgaaaccttc tgggatattg cagtcttatg attctccaca ttagtctttt
     6421 caaccgttac tccgccggca ttattaacct tggacaattc cttttccgca gtctttgcga
     6481 ccgtttcctc aagcttcttc tcctgctttg gattcttcgc ggaaacaatg acaacgattt
     6541 catcttctgc ctcttcgttt atatagccct tgtcagaagc agtttttata atctcggaca
     6601 gcgcagcatc aatattccgg ttcttcaggc tttttgcatc cttaattaaa tcaaccgcat
     6661 cttccgtcag aggattcact gatattaccc tttcaaacct gttcagggcc aaggacaaac
     6721 tgggattaat atctacgctt acatagctgt atggggtgca atatgcatat actcccgttg
     6781 acaggaatat gcatgccatg aagcaggcag ccaatccggc caggtgtttg cttatgggat
     6841 atatcctgcg ctgcttgagc tctatttcat ctcctattgc agccgacatc tgtctcttaa
     6901 ctttaacgaa gtctcccttg tcggttatca ctatcatatg tttcttatct atttctgcta
     6961 ttatagcctt cactttttcc acccccagtc tatgaactcc cttatacaat aataatctcc
     7021 cgtcaaaatt atcattgctg ctattacata atttcgtgag cgttctatgg ttttccgggg
     7081 taattttagt ccttcttcaa tatctgctat gggaagatat tttttttctt ttatttttga
     7141 cacaagctcc ggcctgtcga ttatgtactt cactatatcc aggtatgaac tctttgtatt
     7201 cgtatgcttg ggagagcttt ttgccacatc tgaaaattca agcccccaat tattaagctc
     7261 ttccttcagc tgtacaattt cctgtcttct gagctggttt atctcctcca gatagtattt
     7321 attctgagct tcgggagccg caataacata ctcgaaaact tcttctccgt cagcttcatc
     7381 ggtggagtat tcactgacat atgtaactgc ctgatgcttc ttttctttcc tgtagtaatc
     7441 aatcagtctt tttcgaatta tgccctgggc aaagcttaaa aagtgccctt tctcctggtc
     7501 ataatgaatt atggcttcgt caaaagccat gagccctata ctgagttcct catcatatcc
     7561 gtaagagaca tacctgcccg tagtcttttc gacagcggca gctataaagg gcttgtattc
     7621 ctctatgaaa gcatcccttt catctctatt aagttttatg cccattactc tttcatttat
     7681 ggatttataa aacatggcac acctcaacag aagaacgcat gcatacgccc caaaagtgtc
     7741 tctgtacaat tatacattat ccggacaagc ccttcacgta acatatattg gtaaaagact
     7801 aatattccaa tatttttcca ttccaaaaaa ggtatatgta tttccccgat acctctttgc
     7861 cggttatttg actcagagcc cgcgcatagt attcgatctg tgtcctgtat ttttctttta
     7921 tcctgcttac ttctccttca ggcacgtaat cagtcttgta atcaaccagc accagctttc
     7981 cctcttcttc aaaccagcag tcaattattc cctggagtac aacagtctca ccttcatggc
     8041 cgcagcctgc caaacttccg aacacctcac tgcattccac ttccattgta aagggcattt
     8101 ccctgaagag ccggtcagcc ttaatcatcc ttttgcccag gcttgaattt aaaaatccta
     8161 ttatcccgtt aatgttcaca ttccgggcct gaacctccgt cagaagctcc atcctgatca
     8221 tgtcttcaag ctgccttttt atgccggcct ccgatactac tccgccaaaa tccatatgct
     8281 gcagaacaaa atgcattaaa gaacctctct ccgcagcagt cattcctgat gtttcctcca
     8341 ggaaccgggg cctctttctg attcccgggg cagcctgcat acgaaactcg tcagccgcct
     8401 cagtatcaaa gtatctcttt agctctgtaa cggtgacctt caccggcagt ttctcataag
     8461 cagaataagg atatttccac tccagccttt taattatttc atccttcata ttggcttcgg
     8521 cggaggaaag agccgcttca tctataagct cttccttgcc atgctcttca acaactgctc
     8581 cccttaagac ctcttccctg ttccaaaatc tgatatccca tccctcaaca ctttcaagcc
     8641 tttcggctgg ttttccttcg ttatccctca taagagccgt acatatccag tctaaaaagc
     8701 ttctgcccct taatacatca aactcaggta ctttttgtgt atttgcaccg ctgctgcttt
     8761 tccagcggtc agtactcctg gcgatatctt tcacggctcc ggttattata agcttttcct
     8821 tcgccctggt aaaggctaca tacaatatcc tcatttcctc cgagaggttc tccaacttta
     8881 tcttatattt caaagcctgc ttaaatattg aaggatacca tatcctcctt ttgtggtcaa
     8941 taaaatcagg gcctatacca agttcgtgat ggagcagtat gttctttgcg gtgtccgtaa
     9001 ggttgaagcc cttccccgcg ccgcacaaaa atacaacggg aaactccagg cccttgcttt
     9061 tatgtatgct cataattctt accacgtcgt ccttttctcc aagaaccttt gcacttccca
     9121 tatccccctg gttccccttc agcttgttta taaagttgat aaaattgaaa agacccttaa
     9181 agcttgactc ctcatactgc cttgcctttt caaaaagcat cctgaggttg gcctgtcggg
     9241 cggcccctcc cggcatcgct ccgacaaagc tgtaatatcc cgtatcattg tataaatacc
     9301 atatcaattc atcggtggag agataaaggg atttttccct ccaatcctcc agtttttcta
     9361 aaaatcccgc tgctttacgc ccaagctctc cttccgatgc ggcagctttt accatggcct
     9421 caaaaaagtc cgcctcccgt tcaaagagcc gaatatcaat aagctcctca ggattgaacc
     9481 cgcagattgg cgaccttaaa actgccagca gaggaatatc ctgaagtggg ttatcaatta
     9541 tttgcagcag agacagtacg gttgatattt cgacagtcct gaaatatccc gtacccgcat
     9601 ctgtgaatac gggtatcccc tgaaggctta attcttctgc aaagacgacg gcccaattct
     9661 ttgttgcccg cattagtatt acaatgtctt taaactgtgc cggcctgtat tctcccgtgt
     9721 ttttatccat taccatgaag cctccgtctg ctgtcagctc cttaatcctg ttagccgcta
     9781 ctcttgcttc gcactgtatg gaatttaaca tcaaagccga ttcttcatag tcctcaaaat
     9841 cttcttccgt tgtatctgtt tcttcagcac cgatttcgct gctgtctata atatgaagct
     9901 ccacggggcc tccggcactg cccccgggag attccggcaa ctcatacacc gctcccgcat
     9961 taagcttttc ttcctcggtg tattcaattt ctccaaggct ttcggacatt atccggctga
    10021 ataccaaatt tactgcctta ataatgtcct ctctgcttct gaagttttta ttcaacagta
    10081 tcttcctata cttagactgc gaatccccgg aataagttct gagttttttg ataaataact
    10141 ccggttttgc ctgtctgaat ctataaatgc tctgttttac gtcacctacc atgaatatat
    10201 ttggttctcc cgcctcctcc cgggaaacca tgcccagtat tacctcctga gtcagattgc
    10261 tgtcctgata ttcgtcaaca tatatttctt caaagagttc cctcagccca agggctgcct
    10321 gagtcggcac aaccttgcct tcggtacggt ttgtcagcac cttaaggcag taatgctcca
    10381 ggtcattaaa atctatcaca ttcttttgct ttttcttatc ctgatacttg tcggcaaact
    10441 ctgacacaag gcttccaagg ctgttcataa tgggataaag ctgcctcaaa tcagaggtta
    10501 gttctcccga gccgcatata aggtcttttt ttatcctgtt aagccggtcc ttgacatcat
    10561 ccctggcacc tttggcctgc tctttgataa tagggtctgc ttctttgccg catttcggca
    10621 agcgcgaaaa cccgaggtta tcaaactccc tgtaaagagc atcccagccc ccttttccct
    10681 tacaagccga ctgcattgct tttatccgca aaatgtcctc ctgaagggta tttatatatg
    10741 gagacagccc ctcctccctc tcggctgcct ttattgtatc ctccaacagg cattgaaggc
    10801 cggccagctc catcgagata cttttcatca ggacttccgc ccatttggta tccccgaagt
    10861 ccgtatcctc agccgcatta aaagcttcta tcgcatccct cagccatata tcaggccacg
    10921 gatggctctg cacaaaatca tatatggtca gtacaagatc catcagtttg ttgtcgtctt
    10981 tacttcctcc gtagcattcc accaggctca aaaaatcctc ggtgcagttt tcaggctcat
    11041 attttgattc aataagctcc tccaaagctt ccagttggag cagcaatgct tcggtatcat
    11101 ccgctatcct gaaggccggg tccaagtcta ttgtatgaaa attatttcta attacatcaa
    11161 gacagaagga atgaatagtg gtaatgttgg atttccccaa aagagtcagc tgcctttgaa
    11221 gattcttctt aaccttcggg tttccgtccc cctccgcttc ctccaacacc ctgctcaaag
    11281 cggctccgat cctctctctc atttcagcgg cggcggcatt ggtaaaagtg acaaccagca
    11341 atctgtcaat gtccacagga ttttccgctt gagtaatctt cctgattatt ctttcaacca
    11401 gcacagctgt ctttccggct ccggctgctg cagacaccag caaattgcag cggccctccg
    11461 aaatcgccct ggcctgttct tcagtccagc ccatttgcct caccgccttc cacagtcttg
    11521 cctgccatta gctcccaaat ctcttcgtcc ttcttttccc aaaggttcct gtagttgttc
    11581 ccggaaatac tcatatcaaa ctggcatacg ggcatataac tgcagtacgc gcaggcagtc
    11641 attttctttc ttctataggg gcttatggaa atattccctt tgagtatttc ctctccaagg
    11701 cttaaaagca gcttccttac atgcctcttt attatctcaa actgctccgc cgtagctgcg
    11761 gaagatcttc ccaaaacatc ccccttgtta atccttgcag gtattatcaa tgaatctccg
    11821 tctatgcccg tgtccatttc ttttattact tttacatccg caagaagcag tcccttcatt
    11881 ctaagctcct tcattatcgc cttctctatt tcctcttccg atgcctccct gctgccctga
    11941 ataatagggt catccagcct gaaatatagt acccctgccg gaagcacggg tttgtccggt
    12001 tcttcctcaa ggcttccccc cactgcatcc aggtaggtta tcaactgaag ctgaagtccg
    12061 taatatacat cttccagctt gaaatgcctg cttcccgact tatagtccac tatcctgaaa
    12121 tactttcctt cttctgtttc catgcagtct aacctgtcaa tcctgcccga caggctcatt
    12181 ttttcaccgg agggcagttc caattctatg gccggaaatt cctctccttc tccaaaaccg
    12241 atttcataac ccacaggttc aaaagcactt ctctttatat gctccgctat cagccaaacc
    12301 gcccttgtca gcactctttt cagcctggct gccaagtatt tatacctgtt ggtgcttaaa
    12361 aaaacttttc cggctgcatc ctgtacaagc tcatccacaa taagcgaaac ctccccggca
    12421 caccattccc tttcaagatg acgccagccc agatcgcttt cacttacctt ccgtgagaac
    12481 atatcaataa ccctgtgcat aaaagagccc atatccggag cattcatttt cattacaggc
    12541 ctttcctttg cctttagccc gtattccaca taatatgaaa aggggcagga aaaatatctt
    12601 tcaagccttg atacgcttgt atgtatagtt ttgccgtata tcttcctgat acgctcctgc
    12661 ctgataaatc cggcttgatt ggtatagctc aggccttccg caatccggaa atatttttcc
    12721 ttccattgtt cctccttctt ataccagtca gctacgctct tccacagtgg atccgcatac
    12781 ctgccttcaa atccctgtct cattgcggac accagctcat tgaaggtggg caccggtgcg
    12841 gacacattag tatcagcatc ataccccgaa atacccctta ggattccgct ttcgaagctg
    12901 atttccggaa acatcctcct aagcctgctt attatcacag aaggcctttg cgccctgcct
    12961 tccctgtcgg ccgcacacca gctcagtctc agataatccg aagaggctgt caatgcggta
    13021 tatataaggt gctgctgctc aaaagtcctt ccccgggtat cgggggccaa ttccattccc
    13081 atgttcctca atatctctct gtccctgtcg gataaaatcc cttcctgctt aaaggggacg
    13141 ggaaatattc cgtcattggc gccaagtaca tatatggctt ttacatcatg gcttttggat
    13201 ctttcaaggc ttcccacaag cacctgttca agggccggag gtatcaagcc gatattgtat
    13261 tctccgaagc ccacagagag aatcgtaagg aaggtatccg ccttcaccgc ttcatcccct
    13321 attgcttcga ctacctggtc caaaagctcc aacagaatgt tccacacctg gctgtactca
    13381 tcagcccgat ccaattctcc tctttgggta aaatgctcta ccagcatttc aagtttttca
    13441 ggcacttcca gttcacacag gaaatcaaaa agagccgtac atatttcttt aaccgtattt
    13501 ttgcctttaa ttctcccgta taatcttaac aggggcgctg ccgcccgcct tcttgcttca
    13561 ttgacactca ggccttccca atcctcatcc ttcatccaac tgctccccgg gacatagttt
    13621 gccagcacgt agttctccaa tatgtccgat tctctcctgt caatacctgt aaggcctgtt
    13681 ttcaggtatc ggaaaactgc ttcataggac caattcccta taaatatttc aagggctgaa
    13741 agtacaagaa gtatcaatgg atgccccgta atatcctttt tcctgtctat aaagcaaggt
    13801 attccatgct ctctgaatac cactctgatt actttttcat attggggcag gtctccggtt
    13861 attactgcaa tatccctgta ccggaaccct tcttccctgc actttttcaa aatgtccctg
    13921 gccgcactct ccgcttccgc aaatattccc ggagcttcat acagtttcag gtgctctgtg
    13981 ctttccttgt attttccgtg aaggtatgaa taaaagttgg attcaagaaa tgccagctcc
    14041 ttattgaatt taaaaccatt atttttaagg gatacagggg catccacctt tatgccggag
    14101 tctttcgcca gccttatgag ctttgctgcg gcagcccttg ccggaaggaa ggggtctgta
    14161 tcctccggtg cataatcctc cgaaaggcag tcggtgcaca gcaggacgtt taccctgaca
    14221 gcctttgaca taagttttgc aattactccg tattcctgag gggtgaatcc ggagaactca
    14281 tccagccata tttccgaagt ctcaaaagcc ttgtatagtt caagcttttc cgccaatata
    14341 tcaaggtcgt cgtcagaatc gatgtattta tcatgaagcc ttttctcaaa ctccccgtat
    14401 ataagcccta tatcgtgaag cttatccgca agctgcgggc tgagggaagc tgtttctata
    14461 ctgccaagtg ctgggggcgc tatgttatat ctttttaatt cgcttattgt accgcacaaa
    14521 gtatttacaa agccctgctt cccggcagcc ttggcaaaaa cccgcaattc atcccgcaat
    14581 ttttccatga tcgtatatac cagtatgctt ttccctgcgg ggtttatatg cttcctaacc
    14641 aagcccccca cttctcccag caccctgtgg gaaagcctcc tgaagcttaa cacttccgct
    14701 ccgttgattc cgcttgcccc caccgccttt atgagctttt tctccgcctg gagggagaac
    14761 tgctcgggaa caattatgat atgccttgta ttgttcccct gattcaggct ttctcttatt
    14821 tcgttgagac aatatgtact tttaccgctt cctgcccgtc cgtatatgat tctcaggctc
    14881 attaggaatc cccgcctttt ctggtaactt ttccggtaat atgtatatat tatacatcat
    14941 ataggcctaa gatacgagac tccggatatg ataggagaca gggcaccccc cattgtatag
    15001 gaaagtgtgc tttcctatac aataaaaaag aacgcggacc actccgcgtt cgaggctgat
    15061 gaaaaacctc ctatataaga acagtaaatg agacacgggg acgtttctcc tgtctcactg
    15121 aagtgagaca cgcgaaacgt ccccgtgtct catttgctgt tcctattatg agctttgtct
    15181 gcaaactgaa acgtttaata ctccacgttc tcaaaactat atccaatcat cagtaatatc
    15241 caattattgc agcgcctatt gcccccgcat actgcgagtc cttatgggtc aaaacacggc
    15301 acttcagctt ttttgatata atctccctta tcatgctgtt ggaggcaact ccccccgtaa
    15361 agagcacctt ttctcttatg gttatcctgc ttgccagatg tattatcttg ttggatattg
    15421 agtgcagtat gccggaagca accgcttcct ttgatgtgcc ttgtgaaaga aggctgatta
    15481 cctcgctttc cgcaaaaaca gcgcacatgc tgtttatatc cctgggtgtc gaattggaag
    15541 ccagagcatc caattggtca acctccgttt caaggacatt ggccattacc tgcaggaacc
    15601 ttcccgtacc ggctgcacat ttgtcgttca tcataaattc caatacgttt cccttttcat
    15661 ctattgatat ggccttgctg tcttgcccgc ctacatcaat gaccgtgcgg gcatcagggt
    15721 ccagacatgc cgcccccttt gcatggcagg ttatttcggt aacctttctt gatgcaaagg
    15781 ggacagatac tctgccgtaa ccggtagcaa caacggaggc tatttcccct ttttttattt
    15841 cattctccga gaccagcctc tcatacagct cttttgccgt ttgtctagga ttccagcctg
    15901 tgggcataat aagcctgcct attattcttt catcataagc aaccgccttt gcacataccg
    15961 agcctatatc tattcctata ctaaccatga cagctcctct tcccctgccc ctttttcata
    16021 tgaacttaac ttcagctttc tttctggtca ggcttttgaa gccttctgtt ctgattccgt
    16081 actcttctgc aatacgtttt attaagctgt catcccggaa ttcatacatt atgcttattc
    16141 cgcaggactt gctcaactct ctcggagtag gcactatagt gtatttgaca gctttctctt
    16201 ttaaaagctt ctccagcttc aagccctcgg tgacgttggg aaataacacg tagaagcatt
    16261 cttcctccat tattttaaca tctccaggaa tgcctcaatc cttgtctgaa gctggcctgt
    16321 atcatcattg ctgtagtctg tctctatgaa tattaccggt atccccgctt ctttaagagc
    16381 cttttccacc tgcctgtatt caatgttgta agtatggcag aactgcagat tgcaatatat
    16441 aacgccgtct gcgccatatt cctttgccag ccttacgaca tcctcttccc tacccttgtt
    16501 gggagtgaag catgcacagt ttatattcat atacctgtct gccagggcct ttatcatgcc
    16561 ttccattgtt gtctgggttt catcaaccgt attttcaaag taccttgtcc ctgtacaggt
    16621 ttcttcacag acaaccactg caccgctctc ttctatcagg tgatgcattt tccagtttgg
    16681 tattgccatg ggagtaccgg ttatcattat tctcgggctc ttttccgaag ccactcctat
    16741 tccctttttg atcctgtctt ccagctcatc gcacagctta ttggtcattt caataaatct
    16801 ttcggaatta tcatagaacg ctatctgtga aacaagaagc gcatcctttc cgcttatagg
    16861 aacattaaga gcttttctcg cattatacag tctctgaagt gcagccctct ttctgttgca
    16921 cagcctgata gccgacccaa gcttttcttc cgtaatctta tttcccgtca gttcttcaag
    16981 cactgcttta tattcatata tttcctcttc ccatttctta tagtccttat ccctcttcat
    17041 ctgcggaaga tccataacat aaaccggtgc atattcccca agaacttcat atgccttttt
    17101 ctttccgtcg caggtggttt cgccggtcag taaatcacag gactggaagt acggacaggt
    17161 tccgctgatt tttgcgccta caatagcctt taccagggga caaagatttc gaggcagtac
    17221 tttttcgccg tcctcaatcc agaattgcga gcctccgcag agcccgaccc ctatggcccc
    17281 tgccgcaagt atcagctcgt cgggcacaaa tacacagaag gttcctacta cctttcctcc
    17341 ggccttttta tagtcatcca gttcttttat cctcaacccg tgcacctcag aaaccacaaa
    17401 gttataataa ttcatccctt caggcctgtt ttcctgaatc aggtatactc ccccgaataa
    17461 ttggggcagt gcctcacaaa gcaaatcgtg tttttcaaga tccattccca ggttactcca
    17521 aagctctctg taatctgcca tttttttacc cccttaacat aattgaggca ctatggcgca
    17581 gttaattgcc tataatgccc gtttaacata tttatatttt atatcatcgg attcgaatat
    17641 aataattgtt tttcgctata atgcagcatg atagtattta aaatatgaat atatcccatg
    17701 cctgagacgc tccattgaat tgtctcctta gttctgttag aatctatata tactcaggag
    17761 ggactaaaac attatgaact atataatagc aaaattaaaa aatgatagaa caagatctat
    17821 aatttttatg ataattgcgg cactgctgtg gagtacggga ggcctgctgc ttaagggcct
    17881 ggatttgaat cctatggcgg tagccggctc cagaagcctt gtagcagcgc tgcttatgct
    17941 tgcaataatt aagaagccca gtctgaaatt caacaggtat aaactgggtt ttgccgttgc
    18001 cttcgcagca accatgatat tgtatatagc gggaaataag cttacaactg ccgcaaatgc
    18061 gattatgctt cagtatacgg cacctattta tgtagccctt ctgggggcat ggttcctcaa
    18121 tgagcgtacc tcaaggctgg attggattgc tatttttttt acggttggcg gcatgttcct
    18181 attctttctt gatgaccttt catcaggaaa tataatcgga aacatattgt caatattaag
    18241 cggggtgtcc tttgctttcg ttgtgatgat gctcaggaaa caaaaggatc aatcacctat
    18301 agagctggta ttctggggca accttcttac tgccgcaata ggggttcctt ttcttccggg
    18361 ctctgcaccg gaccagtcag acctggtaat gctggttatt ataggtgtgt tccagattgg
    18421 gctgccttac gtgttttttg catacggaac caaacatgtt tcagccctgg aagcagtatt
    18481 gatacctgtt ctggaaccta ttctgaatcc tgtgtgggtg ttccttctga tgggtgaaaa
    18541 accgggacgc tttgcgctgc tgggcggctt catagtactg cttgccatag tcggcagagg
    18601 tgtaatctta acgttaaaga caaaaaaaaa tccaccggct taacttagcg gtggattttt
    18661 gctgctctat attttttcaa ccttcattcc cgtctgatgt ccgttaagat cctggacttc
    18721 aaagttcgca tccgtcaccc tttctatctt ttcagccagg gtttcggtca tgatgtattc
    18781 ctcatatgcc ttgactgctt ctgctatttc atcgtcccct tcatagtata tgtttattct
    18841 gtccatcatt tcaaaagcat tgttctttct catttgctga accttggaga tgaactctct
    18901 tgcgtaaccc tcttcaataa gctcttttgt cagggtagta tccagtatta tgaagagatt
    18961 gttttctgtt gtcattactt atttctttat atttattatt ctattatttc tgttcggcaa
    19021 aagtctgcgc cataccttta atcatataga cgaatgtcct catatcacca acaagatagg
    19081 tctctttaac atctatagga cgaatttctc cttgacggtt aatccaacaa gtttcatagc
    19141 ccaattcagt agcagggaca acatcatatt tgaatccaaa ccccgcatgg agaatctctt
    19201 cttttttaag ccctaacctt tgtctggcta aatcaaaccc tttgtgagaa ggcttatatg
    19261 ccccagccat ttccgccgta atgacatcat caaactctac gccaagctgt ttcacagatt
    19321 ctttgataat atcattatcg gtattcgtta taagtacaat tttacagtat tttttcaact
    19381 ccaacaaagc atcgcgggaa tccggaaacg gctgccacgt gcccatagaa tcagagaact
    19441 ccacacaatc ttcttctgta aacgcatagc caaattcttt gaatgtcatc gccagggtgt
    19501 cgtgaagaac tttacgatac ggtttatagt ctccctgtat gtagtcaaac tgaatctcct
    19561 cccaacgatg ctgaactgtt ttaggatcaa cttctttaat cccgtttctt tccaggaact
    19621 ttgccatata cttctgaatt tcgccttccc agtcaatcaa agttccaaaa ttatcgaatg
    19681 tcacagcttt aggtaattgt ttcattttta cacctctttt tctttcacta tattttcctt
    19741 tttattctct gccatatgca taaaatcact ttggaaatat gattttgtat cgggctattg
    19801 ttgttttgct gattgcattg tatatttttt ttaacattaa gtaaatatac agttattaaa
    19861 atttgattta agtgttactt aaatataagc gaggagttat atggatagta caaaatgcaa
    19921 agtatttatt acgtcagtag aacttggcag tttctctaag gcggcggcag cattgggata
    19981 tacgatatcg ggagtaagcc atctggtaaa tgcatttgaa aaggaaatgg gttttccttt
    20041 actcacgcgg gataagaaag gtgtaaaacc tacaaataat gcgttgaaaa tcattcccgt
    20101 ttagagagga aacataaata tcttttctaa aggtgtgctg ccaaagatgc aaaatgttaa
    20161 tagcaaaaat attgaagcat aagttttttg aacagctgct gtgacccacc aggtaattgt
    20221 taatagtaaa gatgcataca ctatgctttg gttggtatta aatccaaaag gttgataaag
    20281 aagtataata atcggtgcaa ttaaacttag aatcagcttt tttttcattt tatcacctgc
    20341 caagaccaca aaagaaattg atcttattaa ttatctaaaa atatttcctc tcccaaaccc
    20401 atcaacaaag cattttcata aattttagcc gcacatgcta catcttccgc cgcaattcct
    20461 accgttttaa atagtgtaat ctccttatca gtttgtcttc cgggaattat accggaggca
    20521 acctcaccta attcgccata aatctgtttt tcagaaaaga tcccttttct tatgggaatt
    20581 attagatcac ctgcttcctt caaaaccgct tcccttgaat caaccactat cttatccgcc
    20641 atttttaata ctttttccgg cagctcctgc atttccggag tgtaagcccc catagcattt
    20701 atatgagctc ctttgcttac attttcagct gaaaacacgg gctttttcga agtagtagcc
    20761 gtaataatta tatccgctcc tttaacggct tcatcagtat ttgatactcc tgcaaatctt
    20821 atgtaaggga acttctcgct catttcttct atataccttt tcttcctgtc atcgttcaaa
    20881 tcgaatatcc ttgcttcctg aatatccctt acactgcaaa ctgcccacaa ttgtgtcttg
    20941 gcctgaacac cggcaccgat aacggctacg ctttttgaat ctgtccttgc caaatatttt
    21001 gtcgcaaggc ctgaagcggc tccggtcctg agagcagtta tacaacctcc atccatcaaa
    21061 gccagaactt cacctgtatt ctgatcttca agcaatataa cagagttaat tgtgggtttc
    21121 ccaatctcca aattacctgg aaaaacagaa acaagcttaa cggccacacc gagtccttcc
    21181 acatatcccg gcataaatat cgcatcgcct ttatcctcaa cattgattac tgtcctcaaa
    21241 ggtattttgg tatttccctc ggagaatttg ataaaagctg tttccatcgc actaatggct
    21301 tcatccatag aaattgcttt ctcaatatcg ctttttctta atattaacaa tgaaacccct
    21361 ccattcttac taattatttt tggcttgtac aaaccagctg atatgagtcg ataacgattc
    21421 atacactata ttcataaccg gtttgcaaag cttttttatt cttttcgatt gcactgcctt
    21481 taaagatctt ccccaaatat tcttttatgt tttccagcga taccgaatca atatatttta
    21541 atgctacccc gagagctaca atattagcgg actttggatt tcccatttcg ctggcaatat
    21601 catttacagg aacttttata acattgatat catctcttgt aagttctttt tctaccattg
    21661 agctatttat tatcagataa gcgcccttct taactctgtc tttaaaatcg tccagggatt
    21721 tctcatccat tatcaatacg gtatccgcct cggaaataac aggtgaatct acgctatcgg
    21781 atattacaat ctgacagctg gtacgtcctc cccttttttc ctggccgtat gtaggagcga
    21841 aagtgacttg atatccttta tctactgcag cttcacatag cattttccct atcagcatta
    21901 ttccctgacc gcctacgcct gaaaagatta tctcattttt caattatcat tcacccccat
    21961 atccttgaat acaccaaggg gatatacctt ggacatttct gtttccacca ttttcagact
    22021 atctaacgga ttcactcccc agccggtcgg acattgtgac acaacttcaa caaagcttag
    22081 cccttttccc tccatctggt attgaataca cttttttatg gctttttttg cattcataat
    22141 attcttagga ttgttaacgt atactctctc actaaaatag catccccgga cattggatat
    22201 cagttctgcc atattcacag gatatccgct ggtttcagcc cttctgccat atggtgaggt
    22261 tgttgtcttt tgctcaagca gtgttgtcgg ggccatttgt ccccctgtca ttccaaatat
    22321 cgcattgttt acaaaaataa cactgaactt ttctcctctg attgcagcat gcattatttc
    22381 cgccataccc tccgatacca tatctccgtc accttgatat acaattactg tgttctccgg
    22441 aagagctctt ttcaaaccgg tacctaccga tggggcacgt ccatgcaatg cacatattga
    22501 gtcaaccttg aaattttggt ctgcataaac ggagcagcct ataggccata ccaggatggc
    22561 tttttcagct agtttttctt cttctattat ttctgccaat aatttgttaa ttattccgtg
    22621 tccgcaaccg ccgcagtatg aaaaatgtgc atcagtcaga agctccggtc tcttatatac
    22681 taccttcatt acatccaccc cttaaatgtt agcgttgagc ttatgctctt tatataattc
    22741 aatgagtttt tcgtatagtt cctttggagt tggggtcatc ccgccaagcc tcccatagaa
    22801 actgactttt tctttatctt tgactgataa tttcacatct tccaccatct gaccggaatt
    22861 taattctgta acaaagaaat atttgtcatc aaggccttta aacgcttttt ccgggaaggg
    22921 ccaaagtgtt atcggcctaa tcattccaac cttcatattg ttatcccttg ccatcttaac
    22981 agctgaaaaa gcaattcttg ctgaaatgcc gaaagcaact actactacat ctgcatcttc
    23041 ggttttataa ctttcccatt tttgctccgc atctttaatt ttaataaact tttcctgcag
    23101 caccagatta tactcttccc attgctttgg tgacttggca caagttgtga tgacatttgc
    23161 atctctgttt tttgcaccgg tcaatgccca tgggaactgt gtatcgggtg ctatcctatc
    23221 cggaagctca actgattcca tcattttggc aagtccgcca tcacttagga tcattacggg
    23281 atttctgtac ttttccgcca gttcaaatgc tttaaaagca aagtcaacca tttcctgtgc
    23341 agagtaggga gccaatacta tagtattata atcgccgtgt cctgcacctt taactgcctg
    23401 gaaatagtct ccctggcagg ctactatgtc accatccccg gggcccggtc tcattacatt
    23461 cactaaaact gccggtaatt gcgccgcaca catatatgat attgcttccg cacctaagct
    23521 tattcccggt cctgaggttg ccatcatagc ccttttccct gctgcagcaa ctccggctat
    23581 catatttgac gctgatacct ctgtctcagc ttgtacaaag acaccatttt cttcgggcat
    23641 ttttgctgca taatattcca ttatctcgct ggccggagta atcggatacc cgaaatagta
    23701 tttgcagccc gctcttattg cagcttccgc taatgccata ttcccttgca taaatacttt
    23761 ttctcccatg cctaaacctc ctgcccttca aaatctttga taacttcaat agcagcttcc
    23821 gggcaaacct ttgcacatgt cccgcatgcg gtacattcct tttggtttat gacagctgca
    23881 ggataataac cctttttatt aatactatcg ccttgttcca acactacttt cttaggacag
    23941 aaatttatgc aatatccgca ttctttgcat ttttccatgt ctatttttac taagttcaat
    24001 cagaattcct cctcaactat ctggcagcaa acctccaact ttaatagctg gaggtcagac
    24061 acctggtact gattacctta ttcgtgttta aaatccggat cctgcgctcc tctttgcaaa
    24121 cctttcttaa caacaaggat tccttcagct tttccaacca gcagcggttc tgccagaaca
    24181 tttgcagccg ataaagccag gttgggatct cttgccagct ctattacttt gtatgcttcg
    24241 aatttgcaga ccctggatga gtttcctgcc ttttctatcc atccatagta ttccatgaag
    24301 tcaccggcat aaacaggtgc aaggaattcc accgagttgt aggacaagaa taaactttca
    24361 tctccatcgt acctgataca taattctgtt ccaagatcac cgaaatagtc caatatcttt
    24421 gaaccgttaa ccagttcacc tgcgtaatga gcatcccctg aactcatcct cgctctcaat
    24481 aaaacctttt ttcctaccat ttataaatcc tccttaaatt ttgttattgt ttatattttt
    24541 gcacccaatt tacttgatat ttttttagat gcaattagaa tctgttcctg gtattcttcc
    24601 atccgtccca tattttccgg gttattaggc atagaaatac tgatagctgc tataggatgc
    24661 ataaaactat cgaatacagg tgcagcaaca cattctattc ccaatgcaag ctcttctcta
    24721 tccattgcat aacccttctg tttaaccaaa tccaattcta tgtataagtc tgtgggattt
    24781 gttatagtat tggcagtcct catacttaag ccatattcgc ttacatagct ctcttgtatg
    24841 ctcttagata aaaatgcaag tatcgatttg cccatagcgg tacagtgagc aggatccctt
    24901 cccccaatag ggctgtccaa tctgagatta ttaccgctga ttgccttgtc aatatagaca
    24961 atatcacttt tgtttagtat tcctaagttg atagtttgca aagagagcag tgacagctcc
    25021 tgaagtatag gtcttgctat caccctcaag tccagggtgt caacgatttt attgccgatt
    25081 tcaaacagtt ttaaggacaa tttatatttt ttgctttcac tgtcctgctc caaaaatccc
    25141 tcctgcttaa tgctggcaag aaacctatgg gctgtagtct tgctctctcc gataaattca
    25201 gctacttctt ttacgcttga ctcgccggtt tctgcaaggt attcaattat ctgcaatgtt
    25261 ctcacaaccg attgaattaa gtaatacggc ttcttttcca taatccctca ctaccctctg
    25321 attttattta caaagttaat cttattgaat tccttctgaa agaaatttgt cacattcatt
    25381 gcagatctgc ttctgacctc tcttaatgcg gtcttcgaaa aaaatcctgc atggggtgta
    25441 attatcacat tgtcaaaggt aaataatcta ttattcatag tcggtggttc ttctatcaga
    25501 acatctaatg ccgcagccct tatctttttg ttttctaaag catcggccag atcttcttca
    25561 ttaacaagtc ttcctcttga ggtgttgata aatatcggac attttgccat tttatcaaac
    25621 ttttctttgt taaagtaacc tattgtatct tttatggcgg gtatatggca ggaaataata
    25681 tcagattcag caagcagttt atcaatttca accaatttga cgttcaatga atcagcatat
    25741 tcctgtttta taaacggatc attagcaatt accttaaatc caaagccttg tgctctcttt
    25801 gctacgaact gtgctatctt gccaaaaccc gccaaaccaa gcacctgtcc tttaagccta
    25861 tccatctcca gccctatttc atagttccat tcaccttttc tcacagcttt gtcatatact
    25921 actatgttcc tgctggctgc aagaatgaga gacattgcat gatccgccac ttcatgcgca
    25981 caatagcccg gcacattggc tacagcaatc ccaagttctt cagcagcttt tacatcaatg
    26041 ttgtcaaatc caattgcaga atttgaaata atcttgcatt ttttcaatgc ttttaatgct
    26101 ttggcagtaa taggagcata ttcagataga attgcatctg catcagagca tgcctcaatt
    26161 acctcatcct ctgttattaa ttgatatcct tctactactg cattttcagg caaatactct
    26221 ttttccactt caatatttcc ccagctgcag tcagtaatta ctacattgag actcatttaa
    26281 tcactccccc ttcgtcacac atagtgtgac gtcgtaactc atatgtttac gttagttttc
    26341 tacaaggatt tttatattcc ttctttaatt taaaaaaaaa tttttaattt tttagatttt
    26401 tcttaattaa ataataaggc cccccgattt ttccggagag gcctttatta tttgctttat
    26461 ttcctatatc ctttcaacct tcattcccgt ctgatgtccg ttaagatcct ggacttcaaa
    26521 gttcgcatcc gtcacccttt ctatcttttc agccagggtt tcggtcatga tgtattcctc
    26581 atatgccttg actgcttctg ctatttcatc gtccccttca tagtatatgt ttattctgtc
    26641 catcatttca aaagcattgt tctttctcat ttgctgaacc ttggagatga actctcttgc
    26701 gtaaccctct tcaataagct cttttgtcag ggtagtatcc agtattatga agagattgtt
    26761 ttctgttgtc attgtgaagc cttccttggc tgctatcctg gttataacat aatccttgtt
    26821 tacctccaga ggctctcctt caagctcaat cttcacagtc tcccctgctt caaacttagg
    26881 tgctgctact gctgcgtcca tttcctcaag catttttgcg aaggctttta tctttgctcc
    26941 cagtacgggg cctgcaaatt tgtaatccgg tttcaaggta aagtcaataa atttgttcag
    27001 attcttttcg aatataactt cctttacgtt caattcttcc tggataagcg gaatcaggta
    27061 agatatcagc tcttcatact tggcatctat gaataccttc tgcagaggct gacggacttt
    27121 tatcctcacc tgttccctgg aagctctccc aagtcctaca aggctcctta caagatccat
    27181 tctctcttcc agcttgttat cgatcagatc ttcattaacc tcggggaaat atgccacatg
    27241 taccgacagc tctcccgtta ggttgcggta tatctcttcg ggaaggtatg gagcaagggg
    27301 agccataagc ttggatacac ccacaagtat ttcataggtg gtattataaa ccgctttttt
    27361 atcttccgta agctccgcat cccagaatct tcttctggaa cgcctgatat accagttgga
    27421 aagatcttcg ttcacaaagg tgtgcagttt tcttgccgct cttgtaagat cataaacatc
    27481 gagggcagca ttaacctctg ctgcaaggtt gttgtatttg gaaagtatcc acctgtccag
    27541 ttccggccta tccttgtatt ccacgaagaa atccttcgga tctatgctgt cggtatttgc
    27601 atataatgca aagaaggtgt atacattttt aagtgttccg aagtatttgc tcagtacatc
    27661 ctttacgccg tcctcgtcaa actttgtggt agtccagacc ggtgatacat aaagcatata
    27721 ccatctcaaa gcatctgccc cgtatttttc gaacatatcg aatacatcta ctgcattgcc
    27781 cagggatttg gacatctttt tgccgtcctt atccaccagg tgcccgccca caagtacatt
    27841 cctgtatgga gctgtcttca tgacaaaggt tgatattgct atcagtgaat agaaccagcc
    27901 tcttgtctgg tctattcctt cactgataaa gtttgcaggg aaaagctcat ggaagttttc
    27961 cttgttttca aaaggataat gatgctgtgc aaaaggcatt gctccgctgt caaaccagca
    28021 gtctatgact tcctttactc ttgtcatggt gccgccgcat ttctcacact ttatgtgaac
    28081 ttcatccaca taaggcctgt gcaggtcgat gttttcgtca atatcttcaa ttgccctgtc
    28141 caccaattct ttgatggaac ctattgaatc cgtatgtccg caatcacatc tccatatgtt
    28201 aagaggtgta ccccagtatc tgtctctgga aagagcccag tcattcacat tctcaagcca
    28261 attgccgaag cgcttctccc cgacataggg agggaaccag tttactgcat tattataagc
    28321 caccagatta tccctgagct ttgtcatttc tatataccag ctgggttttg cgtagtaaag
    28381 cagaggtgtc ccgcatctcc agcagtgggg atagttatga tccacctttt ccttcttgaa
    28441 cagcttgccc tctgccgcca gccatttgat tatatcaagg tctgcatcca ttacaaactt
    28501 gccttcccaa ggtgttccta taaatttgcc ctccatgctg acgggctgga gcaccggaag
    28561 gtcgtatttc ctgcctgtat tgtagtcatc ttcgccgaag gcaggtgcgg tgtgaactat
    28621 gcccgtacca tcttcagttg ttacataatc agccactgtc acataaaaag cctttttgtc
    28681 cgcctttaca aagggaatca gctgctcgta ttccatgtat tccatctctg tgcccctgag
    28741 ttcttccagc acttcaaaat cttctcccaa tacttttgca gccagggttt tttctacgta
    28801 atatacttca tcgttctgcc ttaccctgag atatgtctcc tttggattta ccgtcaaggc
    28861 tacattggag gggagggtcc agggagtcgt cgtccatgcc agaaaatact cattggcatc
    28921 ttttttctta aactttacta ttacggtagt ggtctttatc tctttataac cttgggctac
    28981 ctcatgggtt gccagtcccg taccgcatct tgaacaataa ggtgatatct tgtgaccctc
    29041 ataaaggtat ccttcatcct tgaatttctt caatatccac caggccgact caatatattc
    29101 attttcatag gttacatacg gattatccat atcaatgagg aaggccattt tttccgtcat
    29161 gtccctccac attcccacat atttgaaaac agactcgcgg catttctgat tgaattcctt
    29221 gatgccgtat ttttcgatat cctgcttgcc ggtcaatttg agttcttttt caatttcgat
    29281 ttccacggga agcccatggg tatcccagcc gcccttcctt ttgacctgat accccttcat
    29341 ggtcttgtac cggcagaccg tatccttaag agtcctggaa attacatgat gcaatccggg
    29401 cttgccattg gcagtaggag gcccttcgta gaatataaaa ggctctttgc cttctctggt
    29461 ttctacgcac tttttgagaa tgtcaatctc attccagtat tccgatattc ttttttcatt
    29521 ttcttcaatc cggccttctg ataaaaattt aaacttatcc attttataca tcctttccga
    29581 aagtgtgatc tgtaacagca tttaaaaagt cccttcggcc aaagccaaag ggacgatata
    29641 ataccgcggt accacccagg ttatgcatca atcaacaaaa tatgatgcac cacttgacac
    29701 atgtaacgca tgcctgcggg aaggcttact gatttcagcc cttcagctcc gaggtgatct
    29761 tcataaagtg gcaaatgaca atctttcacc aaatgaatgt ctctctttga attgcgtcct
    29821 ttactactgt cctcttcaat gctgttatgc tataaattaa tacccattat tatatatata
    29881 ttccgttaca atgtcaaatg ctattttgcc ctgtttgctg atatttatca atagctgttt
    29941 attaatattg cgccggcctg aaacatccgc ccgcttacaa tttatccaga acggaataag
    30001 ccctgtcaat ataaataatg gcattgcatt caggatattt tgattttatt tcccaggcga
    30061 ctttctttga taggtccccg gccatttttt catcatattc aaagggtacc accaggtcaa
    30121 atatgaggtt cttatgctcc tcacctccga ctatcctgaa atcatgtatc gtcagctctt
    30181 catgcatttc gtttatcagc ccggtaatct gctcctgggt tttttgcact acttcgtcat
    30241 cggtattgat aggatccata tgaatcacca tgtgtatatc catttcctcc gatatttcct
    30301 gctccgccag gtcaattaca tcgtgtgcct tcattatatc catgcttgca ggaatctcag
    30361 cgtgcagtga aactacgcac ctgccggggc cgtagttgtg gactatcata tcgtgaatac
    30421 caatgatgcc gtcatagctt aaaacctttt ccgttattga ccttatgaac ttcggctcgg
    30481 gagcttctcc cagcagcggg ttcaaagtgt cttttgtaag agatattccg gaataaatta
    30541 ttaatgcgga taccagaagc cccacatatc cgtccaaggg aaaagttgtc caaagggaag
    30601 caagtagtga cagggcaaca accgatgtgg ccgttacatc actgaggctg tctgcggcag
    30661 aggcctccat ggctttgctg tctatttccc ggcttatctt tttataaaac ctgctaagcc
    30721 agactttaac acctatggat aaaagcaata cggaaaaggg tattatttca aacttgactg
    30781 tctcgggatt ccttatcctg tcaaaggagc ctttgaaaaa ctcaaaacct gcaagtatta
    30841 ccatgaagga aaccaccata gccgatatgt actcaatcct cccatggccg aagggatgct
    30901 cccggtcggc aggcttgctg gttatcttaa aaccaattac cataattatg gaagaggcgg
    30961 catcggaaag attgttaacc gcatcggcgg taatagcgat gctgtttatc ataaggccta
    31021 ttgcaatctt tgcccctgaa agcatcacat tacaggcaat acctacgaaa cctcccaggt
    31081 agccgtattt ttgcctgaca cttttatccc tgatattatc tttatccttt ataaatgact
    31141 ttattaaaat atctgtaagc aatataacac cccggattct ccatataatg cgatagtttc
    31201 catatttata tattatacgg ctatggcaga aaaaaagcca gtctcacgac gctctttttt
    31261 cgctagggaa ccctatggtt cccttcgacg ctgctctgca gctgagcacc ttccttgaac
    31321 ggcagggggc ttctccccct tgcatccccc ctcgtatagg aaattatact ttcctatacg
    31381 agaacaaaat caaaagaaaa atagaacgct atattttgcc tccggcatta tctatgcata
    31441 ccttgacctc aacactgatc tccgactcct gaattattct gtcaatatct tctttagata
    31501 tcttttccct ggaatgtcct tttatctcta ttcccagttc cagtgcatcg aaaccgtact
    31561 cctcctgcac tatggtgaca aaatcctccg cccttttcct gattaaatcc tccagggatt
    31621 tctggcagag cttgataaaa tccttatgat caataacagg ctcatttttc ttacgctcat
    31681 ataatcttcc cgatagcttt atcaatatgt tgaaaatata cctgtctcct tcttttttta
    31741 ctttaacctt acgcttgttc ttgccttcaa aggttatctg gcctatgacc ttattattct
    31801 ctataatatc ataggtaatc atccctctgc tccggattcc cctcagcatt acagcagttt
    31861 cggttttttc cctgtctagg tgcacaatcc tttttccccc atttagaagg caggagcctg
    31921 caaacacaat attcccattc tcatgcacaa tgtagggcag caatggagcc gtatggtttg
    31981 aaataatatg cctattgctt tcaaacagcg tacaggtggg gaaaaaatta gtcctcctta
    32041 tatttttaat aagcttctgt atatatatgc ccggattggg atactgcttc acaggcacac
    32101 tcagcatatc agcagccttt ccctttgata ctgccattgg aatggctccc ttaaccgctg
    32161 gattccttga taatatatca gtcagttcaa taatgttctc attactgata agttcttccc
    32221 ccaggacaac tacctgaagc tgtcccggta tgagctgctc accgaagtgg gtgtttcttc
    32281 tgtttactac ctctccttcc atagaaccgg aagttatatc aacatgatac ttctgcagcg
    32341 tatcttcata gaaaatcgga aaccctgcgc ttataaggta tttgtcgtca ggctgctcct
    32401 tttcctcttc attggcatag tcatatccta ttatgatagg aattacaagc tcatccaact
    32461 ccctttcatc ccaacagccc gctaagaata gagcattgat aattataacc gaggacaata
    32521 aaatatatct atgtcttttt ttcatctctt gccctccctt tgccccggat cagcgaaagc
    32581 cccagcaaaa gcagcggcag tgcagttccc agtatcagcg aatacatacc tacaaaatct
    32641 gataggcgaa atacaatgcc tatgtttttg ggataccatg caattgcagc aataatagga
    32701 tatagcagtg ccacaacctt gctcagggat tttatgccaa atgcatcctt tataataagg
    32761 ccggcacaga agtattgatt gctgacaggc cttatggcca ctcctatcca tgctattatt
    32821 gcaataaatt ctatcctttc cagcacggga atttccgtaa cggaaagaag cctcagggtg
    32881 ggccatgtga acatttgagt aagttccggc ccgaacacgg ttacagtggt aattaccgca
    32941 tacaggtaaa tcattattgt tattattagt gccagcacgg ctgccttcag ggtctccttc
    33001 tttttcaata cataaggagc aaatacaata atagcctcga agcctgaata agcaaagccg
    33061 gtagtaaagg ctgccttgga atactcggcc agattattat tcagcacagg cataagataa
    33121 tgaagatccg catattttac cactgctggt attgataaaa aaagcggcgg aagcagaaca
    33181 taaaaaagaa actcatttaa tcttcccaga accttgattc ccccgcttgc catataagcg
    33241 cttacagcaa gcccaagtaa tattttaaca agaattgggg tgttcggaag cagaaatatt
    33301 gtgattactt ccgtaaacac cctgagtatt gctgccgaag caatgacaga atatgttaca
    33361 aatactgcag ccagaagctt tcctgcccat tttcccgcaa gcttctccgt aagtgcagca
    33421 aaggataagc ccggatgtct gcttactact ttgtttgaaa gccagataga caccaaaggg
    33481 ataattccgc ccaaaattac tgccagccac gcatcctgtc ccactgcctc tgccgtgacc
    33541 ctgggaagac ttagaatccc cgcccccagc atacagctta taagtgtgaa gaggagctgc
    33601 ttacttgtaa ttgtatagct catttcagtt ttcaccggta ccactcttct ctctgttgtt
    33661 tactgccctt ttatcatcca tatgtgggat tgacacaggc cttttgacat gcatccacaa
    33721 aggcagccta agcagagcat ccttcatgtc gctgaacctg aagggtgcgt atggagaaag
    33781 gtatggcaca ctaaagctct ccagcctcgc aagatgtgtc actattacaa ggagccccag
    33841 agctattcca taggcaccga atacagcgga caataaaatc aggaaaagct tcagcagccg
    33901 catggacaat gtcatactgt agttgggcat cacaaaattg cataccgtag tgattgctat
    33961 aactactatc attgcagggc tcaccagatt ggaccttatg gcggcgtctc ccagaattat
    34021 ccccccgact atgccaagag tccctgccac ggacgtagga agccgtatac ccgcctccct
    34081 cagtatttca actgtaaact ccataagcaa tgtttcaatt acaggcggaa aaggcacctt
    34141 ttttctcaat cgggcaaagc ttataagcag ttccgaaggt ataagctccg gatgaaagct
    34201 ggttagagct atatatattg atgtgcctaa aactgcaatc aggaaggcaa tcaaacggat
    34261 aatccgtgaa aatattcccg cgtgaaattt ttcatagtag tcctcggttg cctgaaagaa
    34321 ctcgatgaat actgccggag ccgtgaacac aaagggcgac ctgtcggtta ttatgagaag
    34381 ctttccctcc agcaggttcg ccactgcctt gtccggcttt tccgttccca ggagctgggg
    34441 gaaaacagac cacgggttgt cttcaatata ttgctcaaca taaccgctgg aaagtatggc
    34501 atcaatattt attttattta tcctcttttt tacttcatcc actattttgg aatccgccaa
    34561 accatttata tatataattg ccacatcagt ctgcgatctc tgccctatct tgagcatctc
    34621 aattttaagc atcgggtctt tgataaattc tcttatcatc ccgatattta cccaaatatc
    34681 ttctatgaaa ccttccttgg tgcctcgtat ccttttctct gtttcaggct ccgatatctg
    34741 ccttttttgc ccgccggcga cagtaaagat aatgaagccc tctattcctc ttacaaggag
    34801 aatcccttct gcggaaaaca tatcctgcag ggcggtgtta taatctttac tgtattttac
    34861 gctgcctgtc tgaaggcatt tttttgtaat atgctccttc agcctttcac ccctcgcctc
    34921 gatatctccc agaccgccgc atgtcatcag cggctttata acgctgtcgc tcagcagctt
    34981 tttgtcgatg ttccccatta ggtatacaag caatgcatct atgttctcat tgccgccgat
    35041 aaaaagctct ctcattatca catccgcatt acccgagagg tctttccgaa gcctgtcggc
    35101 ggtacggtca aagaagtccg aagaatctgc attatttccg ccggctgatg ccttcgtcct
    35161 gaattttatt gtactcatcg gcttcactgc ttttggggca attctgtact tcttcatgga
    35221 aactctccct caatgattat gcgctgattt tctgtaataa aagttattgt ttccaattaa
    35281 agcttattta ttatgagaca cggggacgcg gggacacggg gacgcgggga catggggacg
    35341 tttcgcgtgt cccactggaa gtgggacagg ggaaacgtcc ccgtgtcccc gcgtccccat
    35401 gtccccgcgt cctcctataa aaaagaagcc ttgcggcttc ctatattcct tctctatata
    35461 aaacctccag catcattttc aaaccctgga gccctatgtt cagtacccgg ctgtccttta
    35521 taacatcgga cagcttttcc tcaaccattc ttatcttctt ttccctgatg aactccttat
    35581 tctcccttga aacaaagcat ccctttccta tgacagtctc aataaagcct tccttttcaa
    35641 gctcatcata ggctctcttg gtagtgatca cacttatatg caaatcggtc gccagcttcc
    35701 tgatggaggg cagagcttcc ccgggcttca gctgctgatt gataatctgc ttttttatct
    35761 ggttcgctac ttgctcatat atgggttcct tcgtcgaatt gctgactgtt atatccacat
    35821 atatacacct cgcctctttg aaaactgact gcgagacagg atttgtccca tctgtcatag
    35881 atgactatat acacatgaag tcctgaaatt gtgtattctg cttatagtgt gcatactgtt
    35941 catactgtgt atgccatata tgtacagtat agacattttt gacctttgtg tcaataaaat
    36001 tggtgaaaga tgtagaaaga tgcgaatgaa taatataagc ggggttaaat tagtataatg
    36061 tatgtacata tatgcgtaaa tatattgtaa aaagtacatg attcaaaaat agggaggaaa
    36121 acaattgatg cgcagtgtca aaacccttgt atatactgct ctgattctag gaattgcatt
    36181 gtatttaagg cttcgcttta taatatatgc tccttttgaa ttcaaaatcg acgcctggag
    36241 tatgtataac tatgccttaa atgtaatgaa cggcgaatac tacggacaca gctttggaga
    36301 acattggtca agatatgcct actggcctcc tctttatatc ttcctttccg gttttgtcta
    36361 taggtttttt ggcactgcgc aggattttat tgtaatgcgg gtaatacagg caggtttttc
    36421 cgccgtttcc tgccttctct gctttataac agccagggag gctttaaggg attacagcga
    36481 tctccatgag gaagccgggc ttactgcagg catactgatg gctctgaata caaggatggt
    36541 ggcctatacc aaccatttgt acgctgaaac ggtattcatc actatttatt tagcggcagt
    36601 atacttctcc ctcaggtatt ttaaagacga aatgtccctc aaactcagtt gtcccaatgg
    36661 ttccgtgcgg gttaaacacc ttgttttgtt ctccattttc ttcgggctgg gcaaccttac
    36721 acgccccgtg cttcttctgc ttcccgctgt catattttta tctgcattgt tgtttattac
    36781 cgccagaaga ttcagcatct gccggggcat taaggtcctc gcagcggatg gagccctttc
    36841 actgcttata attcttgctg tgctctctcc ctggatcctc agaaactatg ccgttacagg
    36901 ccgcttaata ctgatagata ccaacggccc catcaacttc tacatcgctc ataatcctca
    36961 ttccaacggg acatgggttg acataaagaa ggcacaatcc gatatgttaa agctgtatga
    37021 gacgggctat aaggaaggtt tagtttacat aagaaataat attaacaggg aaatagatct
    37081 cctaaaggtt aagcagcagc tctttcttta tcatggagat tatcatatct ctgaggctgc
    37141 aacccatttg gacagaacct ataagcttcc cctttacggc agcttgatta agatggagtg
    37201 cccttacagg cttcctcaaa tatcctttga attcctatgg aaatctgctc tcttgctcct
    37261 ggtgcttttc atattcaggc ttatatctgt caaatccctg ttcatactat tttgcgagcc
    37321 tttatggata atagggaact tcctgtatat gaaccttatc attctgattt tttattatgc
    37381 tccaaggtac agaattactg cggagccctt tctctgcatt ctcataggaa tcctgctggc
    37441 gggcatattt acttcgtata ggaagaataa cgcgatataa aattaagggt ttgtgaagta
    37501 ctccgactgt aggggaagta tgttctcctt accttccaaa gcgggggatg caagggggag
    37561 aagccccctg ccgataaagg agggtgctca ggatgcttgc atccgtcgaa gggaaccata
    37621 gggttcccta gcgaaggaaa agcgtcgtga gactagcttt tcctctgggg tattcccgtt
    37681 ccgaggtcag gtggaaggtt tcgtcgaaga tatcttcccc acgtcggtat ttgcattttc
    37741 ttgatatata aggcagtatt ctataagtat ataaccaagc aagagcttgt atagtatagc
    37801 agggcacact tttatgcatt ttggcaacag gcctctgata aggctttggc aaaaagttcg
    37861 tgaagccgac aggaggtcgg cttaggtgcc tcgggccatg gagggccttt tggcaccgtt
    37921 agaacttttc caaaggcata tcagtagccc tgtcaaaatc ataatctgtg tgcctcgata
    37981 tactatactg tctcttgctg agggttatgc agacttcaaa tattgcgcat tattcttctt
    38041 atcgctacta aatataaagt attattatac tgaccaatat ccatagcatt atgtcaacaa
    38101 tcagaggtat gtcggtcagt attatttcct ccggactttc tcccattttt ttattgtata
    38161 cgatatattg gtatctgaaa attccataaa gaacgaacag gatggtcacc attgaatgct
    38221 tgccttcgct ggcataaaac gtataaaggc tataggatat caatgtggat gccgttacaa
    38281 tcgaaagcat gttgtcaata aaatccaggg aatagtgctc cagattgttc ctgtggttat
    38341 gggcatccgc ctgaagcatc agcagctcat ttctcctttt gctgaagccg agaaacagtg
    38401 acagcagaaa ggtgcacagc agcagccagg gtgatattgt caccccaata actaccgccc
    38461 ctcctgaaac tctcagtatg aagcccaggg caatggacat aacatcaaga ataacaatat
    38521 ttttaacata cagcgtatat accacattat taagcaaata tgtcatgact ataaaaccaa
    38581 agctgtaatc cagcataatg gataatataa ctgatacagg aagcaatatt atcatcaata
    38641 tttgtgcttc ccttctgtct attatccctg cagctatggg ccgcattctc tttctcggat
    38701 gctgcctgtc tttttccgca tcactaatat cattaaatac ataaactgcg gaggatatca
    38761 tacagaagca cagaaaggca tataagacag tcataacata ctgcggtttg tccatgttcc
    38821 ttgaaaacag cagtgccgca aacagaaaca ggttttttat ccactgcttt ggcctcagca
    38881 aatggataaa gcccttgaat ttggcccagc cgtccagcct tttcacccct tccatatcca
    38941 taacctcctg tttaattcta ttcgggaagc cttgcacata attagcgatt atgctgcagg
    39001 gtgccagttt tattttttcc tctgaagccc tttaaattcc cgttggaata aaatacatct
    39061 taaggcataa aaaaagcgcc ttacggcgca attttacggc ttgtatatca ctctttcatc
    39121 tatgtcttta agggtgctgc agcctgtcat tatcattgca tctttaagct cagcaccgat
    39181 tttccttgta taaagctcaa ccccctctgc accgccgccg taggctgcta ttgcataagg
    39241 ccttccgata agcactgcat cggcgccaag agcaagtacc ctcacaatat caattcctgt
    39301 tcttattccg ccgtcaataa atattttgat ttttcctcct actgccttgt atatttccgg
    39361 caatacctcc accggtgcgg gagtatgatc caataccctt cccccatggt tggaaaccac
    39421 tatgccatac gcccccgctt caacagcctt ggccgctccc gcagctgtca taattccctt
    39481 tattatgaag ggcagcctcg taccagcaac tatttctcta atttcttcaa caggtttcgg
    39541 actaacgggt ttgcctaaag cagccagaag agcgaggcct gcggcatcaa tatccatagc
    39601 tgctgctaaa gcccctgctg cttccgccat tttaattttt ctcaaaattt catcattctt
    39661 ccatggcttt aatgtcggta taccccagcc cccagcttct gatatagcct tcaacggctg
    39721 ttcgtagaac tcatctttta ctccgtcgcc ggtgaaacct gcggtacccg cgttcttaca
    39781 gcctgataca attgcatggc tgtattcata gtcatttgaa tcttccctgt aatgctgctg
    39841 caaacctccg ataggagctg caaaaaccgg ataagcaaag ctcttgccaa aaagctctat
    39901 agatgtatcc acttccttgg attcatatat tgtatccata ttaagcttta tctcattgag
    39961 cttctcatag ctcctaataa atccgcttcc gctgcccttg ccgcccactc ccggtatttc
    40021 acccctgcag actactccgt tgcattcacg gcaaactctg cattttgatc ctatttgaga
    40081 ctttgcttta tccaatactt cattgtaatt cataagcgac ctcctataat attctttatt
    40141 tctgttaatc ttctctgccg aatacgaaat ctccttcaac tgtctgttca cagtatgcca
    40201 attcctcatt gctgtccgta tcgtatatgt acccgtaaat atagcactct tcgaattccc
    40261 cggctatatc gctgcatagt ttttccatga agttttttaa ggacttatca gaaagcgaat
    40321 tccactcttt cctaaacctg tctctttgta tatatgctgt aaattccgta atgttgtcgt
    40381 ttcctttaag ggtaaaggta aaatggattc cttttaaata atcggcatat tcattattga
    40441 attcttcctc caaaatactt atggcgctgt aattttcata gttccctttg acaagctcac
    40501 cttcccaatt atagtaaaaa gaaaacaata ttcttgaatc cgatatatcc ttgtaatagc
    40561 ccttgatttt tgctgaagca tattcctcca ttataacttc ggcaacctct tcgacaagtt
    40621 catacttctc tttcgccgac aggctgttcc aggatagcct gtccctgctc aggtcaattt
    40681 caaatatagt ccttacctca tcttcatttc ctgaaagtat tactctatat gatactccct
    40741 cataattccc gaattcattg ttaatttcct cctgaagctc ttcaatactc agcctttctg
    40801 cactttcagc tttgctttcc agctttttta actttacttc aagctcttgt atggttttat
    40861 cctttaaggc aagctcagaa ctcaggtact ctaattgcgg atttgttttg tcagtaatta
    40921 ttatctcatt tttacttgaa tcccagtata tatttttttc gaacaaggaa gataccgctc
    40981 ttacagaaag atagttgctt ccgtttatca ataccggctt taatccgaga accgcttcct
    41041 tgccgttata tataatcctg gcgtcagact cagttccctg cacaatcttt cccgtatcct
    41101 cggcaaatgc aaatattaaa aggcaggatg ccaatatagc agcccatatg gtcgttaacc
    41161 cggtcctttt catatattac ccctcctttt attcggttag caggcctatg tccctatatt
    41221 caagcctgtt tctcatactt tcaactgcat attcgattat cttcccatga tcaaaagcaa
    41281 taccccttga ctctgatatt actctgctta cccgtgctgt attcccgaca aaggccctgt
    41341 caacttttat aattgcctcc agggtatccc tgtcattact caatacaagc cttacattgt
    41401 ttatgggttt tttctgatca atgctgctgc actccacctt gaaccatgcc gcatcctccg
    41461 cgtcatctcc tgcctttacg gccagcttgc tgctgtcaac aatagccata tatgaacagc
    41521 ctattaccct ggtgcgggga tctctgtcaa catcccccca tgtatatagc tgctccatac
    41581 atatatcctc aatgcccgtt tcttccttca gctccctaag ggctgccgca tccaggcttt
    41641 catccatatt tacaaaacct ccgggaagtg cccaatatcc aaggaaggga tgattccctc
    41701 tttttaccat aagtatcttc aactctttct tcatttcgcc cgtaattgaa aaaacaagca
    41761 tatctactgt aacggaaggc ctatcatagc gccctgcatc ataacgggcc aaaaattcct
    41821 tctcggtaat gccctgtttg tttcttaaat caccgtattt catagatatc tcctttcttt
    41881 cgctgttaat aaaaactccc atcggtaaat cttcctttgg gagttatttt ttatgcatta
    41941 tttattagcg ccgcagcact ttttatattt ctttccgctt ccgcaagggc agggatcgtt
    42001 tctccccggc tcttttgatt ttatcactgt tcctgaaagc ctttgcttct ttgttatttc
    42061 ctttcttttc ccgtcatcca gtatattgtc ccactgaggc agattataca accagtctgc
    42121 ttttgccttc agcatgttat aataaagctt ctcaaagtct atctccagtt tgatgctgct
    42181 tgactcttcc agcgcctcaa gctccatcgg attaacaaga ctgtcgttaa taccgtcaag
    42241 gaagcccgtg aacagcagta tatccgtccc gtacctttct gccagttccg ccaccgttcc
    42301 ctcaacaacc tcttcgggat tggaaagcag cttctcatag atggattctt ccttctcaaa
    42361 gtatccttcc caaaacttct tttttgcctt ttcatcctgc tcgttgttat atgcagtatc
    42421 tctccagttc tcaaacaaac tcatgctact actccttatc cagtgatttt atcatatagt
    42481 aaatacgcct gttcggatat gcagtaaggc catttccccg gcgcacttat tgaatttcct
    42541 taattattat attacattgc agtataattt gcaatttttt gtctgattcc agtctttcat
    42601 accttggttg taatgctgtt gtcaagcatg ctcaggtgtt tgaaaagctt tccggataca
    42661 ttcattattt ccagcccgtg ccgatacata tctcccattt tcatcatcca cattaccttc
    42721 gcagatactt tttcgtattt cacttcttcc agcttgaagc ttagtatcag caccgcatct
    42781 ttctcaaaag gcttttcaga tattataccc aaccccccta ctgaaatatt aataagtgta
    42841 agttcaagcg gtatctcata ttcttcggtc ctgccctcgc aagtactttt aatgcattgt
    42901 atctttgaat aataatcaat ccttctgatc tttctcttat tggcaggctt tgaagcatcc
    42961 gtaactttac tcttcataat tctgccctcc atatagtatt attaattatt ataccatccc
    43021 attgcccaag cagtattcct ttttatatga ttggccgtcc tgttcataca aaaaaaatca
    43081 ttgacatttt gccatacagc taatataatg ataaaaatat aaattttcga caatgatggg
    43141 aagagtagct tgtaggatat gccaaagcga gccgggatcg gtgcaagccc ggtgctaagc
    43201 ctgaagtgaa gaacagcctt gagtctccaa ccgaaatcct ttggatagta gacttggacg
    43261 gagccatacc gttacagaat gggcagtatc gatttctgaa atgcatcaga atatccgtac
    43321 tggcagagtg gattggagca taacttcagt caatcagggt ggtaccgcga ataacctttc
    43381 gtccctatta ttgggatgaa gggtttttat ttttgcagca ataaaaagga ggtattaaaa
    43441 tgtcagaaat gtattcactc agtataagag aaacggaggt agcaattaag gtaatcaaag
    43501 atttttttga agcgcagctg gctaaaaagc ttaatcttac aagggtctcg gccccactgt
    43561 ttgttgaaag ggctacaggc cttaatgaca atctgaacgg aatagaacgt cctgtttcct
    43621 ttgatgcgct ttccctgaaa ggctcggagc tggaaatcgt acagtcactg gctaagtgga
    43681 agcggcttgc tctttcaagg tacgggttcc tgcccggaga ggggctttat accgatatga
    43741 atgcaataag aagagacgaa gaattggata atcttcactc aatatatgtg gatcaatggg
    43801 attgggaaaa gattatcctc agggaggaaa ggactcctga aaccttgaag gctgtggcaa
    43861 aaaccatatt cagcgttttt aaggatacag aggaactaat tttcaagcgt tatccgcagt
    43921 tcaaaccggc tctgcccgag gaaatattct ttattaccgc acaggagctg gaggacatgt
    43981 atcccgataa aagccccaaa gaaagggaaa atcttatcac aaaggacaaa ggagcggtgt
    44041 ttcttatgca gataggcgcc cgattgaaat ccggaacaag acatgacgga agggctcccg
    44101 attatgacga ctggtctctg aacggagata tattgttctg gtaccctgta ctgcaatgtg
    44161 ccgttgagct ttcctccatg ggaatacgtg tcgatgaaga agctctgctg cgccagttgg
    44221 agatatccgg ctgtatgtgc cggaaggaat tgccttatca cagctccctg ctgaagggcg
    44281 agcttcctta tacaataggc ggaggcatag gccagtcaag aatatgcatg tacttcctgc
    44341 aaaaggtcca cattggagaa gtgcagtctt ccatatggcc gggcagcatg gtaagccagt
    44401 gcgaaaaaaa gaatataaag ctgctctaag ctttatattc tgcactctcc tttttttcga
    44461 aaaaattaac ctgggtcagg agcattcctg caaacataag agcacagccc aaatatcccc
    44521 taacccccat attctccttc aggagcagtg cacccccaag ggctgcaaaa actgcttcca
    44581 tgctgagtat tatggctgca tgggaaggtt ttgcgtgctt ttgagctacc acttggagcg
    44641 tatatgctac gcctacggat aataggcctc cataaagtat gggaattgct gcctggctta
    44701 agccggatac tgatatatcc tcgaatataa gtgcaattat gatgcttaat gccgaacaca
    44761 ttgcaaattg tgcacaggac agcctcaatg catccacttt ctttgtaaag taatcaataa
    44821 ccagtatatg aactgcccag aaaaaggcac cgataatctc cagtaaatcc ccgaatgcta
    44881 tggtaaagtc ctcattgaca cttagaaaat acagcccgga aacagccaga acaactccca
    44941 gccaggtatt caaacctaca cgctgcttta agaaaatacc cagcaagggc acaagaacta
    45001 tatataaccc tgttataaag gcggcctttc ctgctgttgt atatgcgagg ccggcctgct
    45061 gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctgtta taaaggcggc
    45181 ctttcctgct gttgtatatg cgaggccggc ctgctgaaga gaagctccga aaaacaaaac
    45241 tgaccctgca atgattcccg gtaccaaagc acttgctgaa gcggtctctt ccggctgttc
    45301 cgcagttttc ttgttcttga aatatattat caacggaatc agtgaaatac ttcctaatgc
    45361 aaacctcacc ccattaaaag taaatgcccc tacatactgc atgcctactc tctgggctac
    45421 aaaggcaaaa ccccatatgg ccgcagtaag cataagcagc atattggact tcatttcacg
    45481 attattcatt atttaatccc cctatcatta gctctgccaa tactatttta acttgtatca
    45541 gccgaaaaaa gcaatggcaa taattcaaaa ggaagcttat tcacatccat agaatctgcg
    45601 ggctattttt accgcttctc tcgcatcctt gccatagtaa tccgccccta tcatttttgc
    45661 ataatcttcg ttaagcactg ctccccctac cattactctt gtatccgggc aatgctgcct
    45721 gagagcctta acggtatctt ccatgctctt tactgtggtg gtcataaggg cgcttaagcc
    45781 aaccataagg gctttctctt ccttgacctt tttcacaatg ctttctgcgg caacatcctt
    45841 cccaaggtca taaacctcga agccgtagtt ttccagcagt atcttcacaa tattcttgcc
    45901 tatgtcatgt atatccccct tgactgtggc aattacgatc ttaccctttg agacccccgt
    45961 aacctctcct gcatttttga gcatgttgtc ctttattgct tcaaaagctc ttttaacagt
    46021 ttcagcagac tgtataagct gcggcaggaa aatttccccc gactcgtatt tctgccctgc
    46081 aacgtcaagg gccggtatca ggaaggtatc tacaatctcc ataccgttaa tctccgtaag
    46141 aagctttttt accgttaacg ccgcttcttc cttcattccg tctactatca atttttttaa
    46201 acttcggttt tcttcctttt ctttgaatac aggctgttca ttgacctcag agcggctgct
    46261 gatgtattcg gctgcttcct tgtcgatatt ccatagtaca ttatatgctt ttattgtatc
    46321 tgtcatggct tctgccatcg ggttcagaat cggagcatca aggcctgcgg tcaaagccat
    46381 ggctaaaaat gttctgttta gcagctctct gtccggcagg ccgaaggata cgttgcttac
    46441 accaagggta gtcttaagcc ccagcttctc ctttacaagg gctacagcct taattgtctc
    46501 ccggatctcc ttctgctggg ctgaggcagt aagcacaagg cagtccacta taatatcctc
    46561 cctgcctatt ccgaactcct ccgccctgtt tactatcttt tcagcaattt tcagccggtc
    46621 ttccgctttt tcaggtattc cgttttcatc aagggtgaga gcaacaacca ggcatccata
    46681 tttttttgct atgggaaata tagtgtccat tgtctttttt tcgccgttca cagagtttat
    46741 tatgggcctt ccgctgcaaa tccttgccgc agcttctatt acatcaggac tgacactgtc
    46801 aatctgcagg ggaaggttca ctatattttg cagctcaaac acagctctta ccatcattgc
    46861 tttttcatct atttccggca gccctacatt aacatcaagt atgtgggcgc ctgcatccct
    46921 ctgtgcaact gcctccttca gcaaataatc catatcgccg tttcgcagcg cttctttcaa
    46981 tcttttctta ccggttgggt ttatcctctc ccctattatt tttgcccctt ctccaagaat
    47041 cactgttgca gtcgccgaac tggccgcagt gagctttact gcttcgggcc tgacgggctt
    47101 tagtcctgat acagctttcc tcaattcttt gatatgttcc ggagtagtac cgcaacagcc
    47161 tccaagtatt gctgctccca tttttgccgt ttcggccata tattgtccaa actgctcagg
    47221 gcttatgtca taaaaggtct tgccgtccaa aatgatcggt aacccggcat tgggctgcac
    47281 aattacaggc acggttgaat gtttgagtac ccgggcaaga aggggaataa tctcctttgg
    47341 gccgagagag caattaatcc ccagcgcatc tacccccaga tgcgatatta tattcaccat
    47401 tgtcaaagga tcggtgcccg tcagggtcct tccgtcaagc tgaaaggaca tggtacacaa
    47461 tacgggcaga tgactgtttt cctttgctgc aaggatagct gccctggctt cgtatatatc
    47521 cgacattgtc tctatcaata tcatgtccgc ccctgcagcc gctcctgcaa ctacttgctc
    47581 cctgaaggca tcgtaggcat cccggaagga taatgtaccg tatggctcca ttagttggcc
    47641 gataggccct atatcatgcg ctgtaagctt gcttcccgct gcttcttttg ctatttttac
    47701 cgcacttcct atgatttcac cgcagctgca gcccgaaccc ttcagcttat atctgtttgc
    47761 cccgaaagtg ttggtggtaa ttatatccgc tcctgcgtca atataggctt tatgtatttc
    47821 aagtatcttt tcgggattgc tgatattcca gctttcaggc agttccccgg ctttcagccc
    47881 atgggcctga agcattgtac ccatcccacc gtcaaatatc agcaactcct ttgattcaaa
    47941 tatactaccc attattccac accctttctg aaaaaacaat ttttgctgtc gcagctttca
    48001 cagctatggc ctgatttatc actcttctcc cgctgaagcc caataaaggc cgttacggac
    48061 tttctcggta tcattatgct gctttcattt acactcagtc ctataccctc gtaagccttc
    48121 aatacctttg ccaattcctt ttgaatccca atagaaaaat ctccgtatcc ggggctgaat
    48181 cttgttgttg tttcaaatcc catggacctc gcctcggttt ctattttctc atgaaccgta
    48241 tcacatagag attccgctgc cgctgttgca caggcatcaa ataccagggc ttttgcaatg
    48301 tccgtcttcg aatagaaggt tatgcgttta tcaacttcaa gccccagagt cactgccatt
    48361 aacgcacatt tttcagaatt ggcaagatgc ttgccaatgt cttctccggc aagggtcaat
    48421 gtagtccctt tcaagcataa gccttccgca gtatgctcca catcaaagat ttcatacaaa
    48481 aagctttctt ttgatacact tgcaacctca tcaatacaat cattcaacag cttttccgta
    48541 gtatgaccga tgttttggcc tctataaccc atataacgca gtacttcttt tctgtctatg
    48601 tacataatca gccctccaat ttaatatatg ataaacacaa actgctctaa aaagcaataa
    48661 gtaatttatt ttctcaaaag tacaaaattt atatattgac attgctgaaa taatggggta
    48721 tattatattt taaatattta agaaccataa tggtgatgat gggaagagta agttggagcg
    48781 agtggcaaag agagccggga agggtggaaa ccggtacatg ataccagccg aacatggtct
    48841 ctgaactgta taagtgaggc agttgcttag cttataccgg caggctgacg ttatacagca
    48901 cggtgataaa tcatttttga ggagtcactt ggtaagtttt ttgtcgtgag gcaaaaaaga
    48961 atcagggtgg taacacgaat agctttcgtc cctttattgg ggatgaaggc tttttttatt
    49021 tactatgcct tgtcttaccg acaacaaatt atattaggag gtatagcaaa aatgacagta
    49081 aaaaagacct ttgaagaaat caatgaaaaa atcaggaaag gtaacgcagt agtagtaaca
    49141 gcagaagaat tagcagacat agtagaggaa aagggtattt ccgacgcaac aaaatatgta
    49201 gatgtagtaa caacagcaac ctttggtcca atgtgttcca ccggtgcctt tcttaacttc
    49261 gggcattccg atcctccgat cagaatggaa aaaattgagc tggacaatgt aagtgcatac
    49321 ggaggacttg cagcggtaga tgcatatata ggagcagcag agccctcaac ggacagaggt
    49381 atagaatacg gcggcgctca tgtaatatgc aatttaatag acggcaaaaa agtgcatctt
    49441 aaggcatact caaagggcac agactgttat ccgagaaaag aaatagatac ttatatcacc
    49501 aaggattcga taaacgaagc ctatttgtac aatccgagaa attgctatca aaactactcc
    49561 gcagcggcaa atacctctga caggacactg tacacataca tgggagtact tcagcccggc
    49621 ttgggaaatg taaattattc tacttcaggg gaactcagcc cgctgttgaa agacccctac
    49681 ctcaggacta taggtatagg aacaaggatc tttttggccg gtgcagaagg ttacgtagcc
    49741 tggcagggaa cccaatttgc cagcggaaaa cccagaggtg agaatggaat ccccctgtcc
    49801 ggcgcctgta cccttgcggt aataggcaac cttaaagaga tgagcactga atatattcag
    49861 ccggctattt ttgaaaaata cggtgtttca atgtttgtcg gaataggaat acctatacct
    49921 gtccttgatt cggatctgct tttcaatctt tcggtacaaa acaaagacat atatacaaat
    49981 attttggatt acagcgtaca aagaagcaat aaaccggtct tggcacaatg cagctatgag
    50041 gaattgagaa gcggatttgt ttccattgaa ggtaagaaag taaaaacttc acctcttaca
    50101 agcctgtata aatcaaggga aatagcccgg gtactaaaga gctgggttgc ggaaaagggc
    50161 tttacattac agcctcctgt ggcccctttc cctaagggag taacgctgaa gtcactggaa
    50221 caagggaggt tatagtatgc aaggatgcat tcattgcggc gcttgtaccg cggcttgcac
    50281 caaaggtgct ttgaaaatgg aatccgaatc ggggactgtc agttgtgatg aatccctttg
    50341 cacacactgc aacgcttgca taagagcttg cccggcaaga gcacttacct ataaccagat
    50401 aaggaggcct tatgtatcag aaaagagatt atcggaagct tatgaaggca gatgatctga
    50461 tatgcttcaa tgtaactgaa tatgaatccg atttgctgat atatgccagg acaaacctaa
    50521 agaaagaagc aaaaattgct gttgcaaaat acagaaagga tattattgat tacattagaa
    50581 gacatccaga gtttttgaca agcttggtgc cgatcaatcc catattggac gcccccgaaa
    50641 taatacagga tatgtgccat tgctcaaagc ttgccaatgt aggcccaatg gcagcagtgg
    50701 caggggctgt ttcaaaatat gcaggcagag atctgctccg atttgcagat gaagtactga
    50761 tagaaaacgg aggtgattta ttcattaaat caaagaatga cagacggata cttatttatg
    50821 cgggcaattc ccccctttca aacaaggttg ccctccttat tcccggcaat aatcaggaac
    50881 tggggatatg cacctcaagc ggaactgtcg gccattcttt aagttttggc aaagcagatg
    50941 cagccgttgt gatttcaaag gatgctgttt tagctgatgc cgcagccacc gctgtcggaa
    51001 atgtggtaaa ggataaaaac catattgagg ccggaataga atatgccatg tctattccgg
    51061 gtgtagaggg agtagtaatc attgcagatg acaaaatggg tgcctgcggt gatattaata
    51121 ttacaaagcc ttgagaggag aattggttat gaaaataaaa gagtttttta agaacaagcc
    51181 tgttatatcc ctggaaatat ttccgccgaa acctgaaatg ccaatagata caatatttaa
    51241 taccattgat gctctgtccg gtcttaagcc tgcatttatg agcgtaactt acggtgccgg
    51301 cggcagcagc aaggctcata ccgttaaaat tgccgatgtc atcaagaata agtataacat
    51361 agaagccctt gcacacctta cctgcatagg cgcaaaacgg gatgaaattg acaatatact
    51421 taagcagcta aaagattata atattgaaaa cataatggct ttgaggggag acatacctgt
    51481 gggttcggat cctgcttcct tagagaatgc cgaatacaag tacgccaaag atctaatatc
    51541 ctatataaaa gaaaaggaca gtttctgcat cggcgctgcc tgttatcccg aaggtcatat
    51601 tgagtgtccc gacaaagtac aagacttgaa gcatctgaag ctaaaagcag attccggtgc
    51661 tgacttcttg attacccagc ttttctttga taataatcta tattattctt tgctggaaaa
    51721 gctcgatata cttgatgtca agctcccggt ctcagccggc attatgcctg taataaacaa
    51781 gaggcagatt gaacgcatca cagctctttg cggtgcctct attcccccaa agattaaacg
    51841 tatactggac aaatacggag ataaccccga agcattgaag gaagcgggta tagcctacgc
    51901 aacagagcag ataatagacc tgct
//
LOCUS       DAOOAB010000013        50474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_836_length_50474_cov_15.542276, whole genome shotgun sequence.
ACCESSION   DAOOAB010000013 DAOOAB010000000
VERSION     DAOOAB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 50474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50474
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_50474_cov_15.542276"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1020)
                     /locus_tag="PKU88_04535"
     CDS             complement(79..1020)
                     /locus_tag="PKU88_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPX68584.1"
                     /translation="MDPVTHAAVGLTIAVMSGEPLAASNPAMIACVAGAIIPDGDILM
                     QLKGDYAYLKNHRGMSHSIPMMFVYSGIITGLLMLAFNGAAFWNIFPLALLGCFSHIA
                     LDITNSYGAQILWPLSKKHITFDLLLVYDPILIILCLINILPYTRGIIPPYMTVFIFA
                     GYLILRRIMKKLAQDIAYQNLGYKYKSEYFRIMPSMIGIIKWHFVLYTGRRKIIGEVN
                     IFPRRFRIIDNKKNIDTDLYKIAMDTPVGMFFEKFTPVFHVECEKKEGNYEFEFIDLR
                     YHISKDFLHHATAVINRDLEVVTSLFHPYRKTRNVEV"
     gene            1210..1295
                     /locus_tag="PKU88_04540"
     tRNA            1210..1295
                     /locus_tag="PKU88_04540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Leu,seq:caa)
     gene            complement(1414..2796)
                     /locus_tag="PKU88_04545"
     CDS             complement(1414..2796)
                     /locus_tag="PKU88_04545"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPX68585.1"
                     /translation="MKRANIKKIASGLLTAALLLTLSFSVDFLIPEAVYADTTGGWHL
                     VETNYYLSTLDYTVKGEIQNGIIGTSDVIYDKYAVEGSEGDMTITHSRRYDNGNPIAH
                     VTYQVVWDTPPSYIASGQKTGFNIDYKVVSSKSWGGVQINAHLDAASIKPGGATSSYI
                     SFQLEDGTKYINSDKQGYFESEKVIPEGRSGDKKAVILTLGNGYGYTYIYEWKEGAVP
                     AVPVVPATPVSAGSGAEPFESGARIMWQPANGLGYRLFRSTTPNELGISVTDFYITST
                     SYADVNVEPKTTYYYSVKPVLAEAKPFEGIDEKLGDVIATFVVTTGDQVYKPGSYKHF
                     IMLKLEDPNMSVDGVSQEVDPGRGTAPVVIAGRTMVPIRAVVEAIGGTVEWDGATKKI
                     TLTARGNTVEMWLGKTDIKINGESKKMDIAPVSKNDRTFVPVRFAAENLNCKVDWINS
                     TKEAVIVYEE"
     gene            complement(2953..4392)
                     /locus_tag="PKU88_04550"
     CDS             complement(2953..4392)
                     /locus_tag="PKU88_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX68586.1"
                     /translation="MRFTDSRSKILLSERWLSVIAHSLFSSWMLSFIFEGEVFYSLMG
                     IFDFDSHAMVFGGIAAVFLGLLLSGFFIKTKRGAKRTFLYSYLFCIVMSVPFFFPPSV
                     LWTVGIIAGSFMSGSCVAAWGYYLKSSTPKIGRIKTVADMLILSNILMILLNMAALWI
                     SPHSGLALSMIMLILAFLFALKLPGDNCISSSASFERDKSRISIAPLLVFLCIFIIVI
                     TINSGLMYQVVNPAFAHLKWLTSWYWAIPYIIAVFIMGHLPQKVNRAYILYVAIAMIG
                     ISFISFMILDHNVAGYIAVNTLMLGAFGVCDLFWWSILGEMLDFHKNPATILGIGLSA
                     NVIGILLGGLIGNAIISVDKQSADPVLLALTIVCVALAILPLLHKYLSSLLRDHTYLK
                     AFSEISAQEQTREPDYNTVFENLSERENQVASRLLQGKTYRIIAVELYISENTVKYYV
                     KNIYSKFKIQSRGELVNIILEKRGIGIGK"
     gene            complement(4580..5911)
                     /locus_tag="PKU88_04555"
     CDS             complement(4580..5911)
                     /locus_tag="PKU88_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPX68587.1"
                     /translation="MDGDLWTQILLIILLIGLNAFFAASEMAIISVRQSRIKPLVDEG
                     NKAAILVNRFLEEPSKLLATIQIGITLAGFLASAIGAQTLTNGLTGFLMSLNIPFLSA
                     SAVGISTFIVTSIIALFTLILGELVPKRMALSQSEKTAFRVAGTINLLSKLTYPIVKF
                     LTVSTNLVVKLMGGPAKVTEEQITEEEIRSMINVGEEKGIFREAETEMINSIFEFDDT
                     VAKEVMTPRIDIVALNSEATREEILDVIVQENFSRIPVYEESIDNIIGILYVKDLFAM
                     IRNNVEWEVSLKEIIREAHFVPEYKKIDELFKEMQKSKTHIAVVIDEYGGTAGIITIE
                     DLLEEIVGNIFDEYDDVILDYEKIDDNTYIVSGMLNVSDVNDILNTKLSGEEEFDTIS
                     GMLLSHSGKMPEVGYELTIDNIYFRIEEVDDKRISKIRIEKRDIEPDKTEE"
     gene            complement(6282..6893)
                     /locus_tag="PKU88_04560"
     CDS             complement(6282..6893)
                     /locus_tag="PKU88_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68588.1"
                     /translation="MAHGKDNNVSDSRMNELKNMVSEKLGGMDIDNVSPDQVKNMLVN
                     EISRNSNIDPAIKDKINKGDVDGLKEDIIKYLSNNRSADGSSDQLINMLKDNDMEGLK
                     KQLMGMLLSGLGPQKKNEIKEPEVYESQADLKDSLGDFDERALLGLLFDKMFAGVKDD
                     SRISLLYSLRPFVSEKRQRGIDDCIKIMNLVAFFENFMSKAGK"
     gene            7195..7761
                     /locus_tag="PKU88_04565"
     CDS             7195..7761
                     /locus_tag="PKU88_04565"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPX68589.1"
                     /translation="MAKQMWKPGTMLYPVPAVMVSCRSREGVSNIITVAWTGIVCSEP
                     AMLYVSIRPERFSHGIIKDTGSFVVNLPTESLAYALDFCGVKSGRDINKFEHLGLTQQ
                     KSNLVDAPSIGECPVSLECRVNNIISLGSHDMFIAEILCVDAEEKLLDKSGKLHLNKA
                     GLICYNHGEYRALAKSLGHFGFSVRKKK"
     gene            complement(7814..9505)
                     /locus_tag="PKU88_04570"
     CDS             complement(7814..9505)
                     /locus_tag="PKU88_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68590.1"
                     /translation="MNNKVVTFERGADFYFDLSYKYIQKGRLKTALRYIEKAVSLKPH
                     DSFLQFNYAGLLAEQGHIDRSTEVLLNIVKNIDPNYLECYFGLGCNYLQLQKIKKSSE
                     YFSIYLEKDPGGEFAEEAEELLEMLTMIKDANNNMDDEELEKIYKIEEEAIDHLEKKE
                     YKQATEKFEIVVQMLPNAVPARNNLSLAYYYLGFIDKAIELALEVRTYEEANVHANCN
                     LAIFYNKKGAASLVKKQLGIISRIRADSADYLYKIGDTYGSLGKHADAYKTFKKLLII
                     DSKNPIYVHLAAVAAFNSRKYRESIRLWEKLGQIDKENMLSDYYIEAAKNQLDAEDER
                     TYFPYLYQLPKEEIKNRLDFIYKFIEMPGPKCREQLADNEHMLGMLYFGINFDKYFLR
                     KLIFNKIKNDRMNEAESFIRRFMLRDDVENYIKMEAVFLLNFIGAKEPYTVNFDGEKR
                     NVTIDSLDFPESEWEKPWIKVKRKAISMMRNKYKRPYKKIVEDIWYEFIKSTFPEVPE
                     IDDVNIWAAALEHTYCRLCNAEASEHKLAENYNISSVSLLEKAEIINEAIKNKFQ"
     gene            complement(9539..10696)
                     /locus_tag="PKU88_04575"
     CDS             complement(9539..10696)
                     /locus_tag="PKU88_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPX68591.1"
                     /translation="MDKQKLYTLLQKPEGTKLDFKAVLSLKTESEKKELAKDVAAIAN
                     SKGGRGYIIYGIQDETKAILGIEGKRYTEEQIQQIISQRCDPPVSVKLEIIPVDDKQI
                     AVLTIYRSSQKPHQIRQTGVFYIRRGSTTDIARREEIASMLQESGILQYERIVLNRVE
                     LKELNEDIISDYISKTGFTSSSENYYTLLEGIGIIGRDEDSNGYHPTVGGLLVFGYNP
                     QLHLPHAGIRLIDKSAADKVIYFSGPIIRMLDEIEDYLRFKAEKINGKYPVAAVVEAV
                     ANAAVHRDYFSPGREIVISIDNKKIEISNPGANCSDDDIQGLMEEYNPCRRNQWLYQR
                     LLILDNKGRFLNIGAGMKKIHEAFKQHGGARFINNEKRNLFKVVLPGFMKY"
     gene            complement(10868..11131)
                     /locus_tag="PKU88_04580"
     CDS             complement(10868..11131)
                     /locus_tag="PKU88_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68592.1"
                     /translation="MTIARVCGSIENTTVRKFDRGGKSMNMIIKLLTGLKCKDRENRD
                     LNDCGYTLYQSLEYNLKYLKGDDFGYRDPRTQSTDDLMARKTF"
     gene            complement(11495..12139)
                     /locus_tag="PKU88_04585"
     CDS             complement(11495..12139)
                     /locus_tag="PKU88_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68593.1"
                     /translation="MSNIRILIADDHSIVREGLKQLLELEEDFEVVGQASNGVETIEK
                     VEELKPDVLLLDINMPIMNGIKALKKLKEDGIDTRVVILTIHEDREYLLETMQIGASG
                     YILKDSDSASFFKAIRDAYIGESYIQPKLAADLIKEFNKPRGARVKNENELTQREYEV
                     IVLIADGLNNKDIADRLFISEKTVKNHVSNIFRKINVSDRTQAAIYAYKNNIKK"
     gene            complement(12141..13283)
                     /locus_tag="PKU88_04590"
     CDS             complement(12141..13283)
                     /locus_tag="PKU88_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPX68594.1"
                     /translation="MDRLNEIIKNTVDIIEKSRSELFSIAESSRTECQLVEEELEKIR
                     IIASDLINQVDMLVKLDKECRYQLMIVSKNIKDYKEEDVKRAYDKAKDLQVELMTKKA
                     EEQLLIQRRSELERRLKIARDTANKAESLVNKVGVAMGYLTGELQDLSIQMEDIKQKQ
                     YLGIKIIKAQEEERKRVAREIHDGPAQLMANLVIKTELCEKLIDMDKEKAKEELGSLR
                     GFLRSSLSDVRKIIYDLRPMSLDDLGLVPTLQRYISNYIEQNNISVNFSVLGDTKTLK
                     PVVELAAFRIVQEALTNIKRHSGADNAVVRVEFTNDQVNLLISDDGKGFDKNKLWKAK
                     EEDAGYGLLNMKERVELLNGKFDVKTARNKGTKVFASLSLNIAEEG"
     gene            complement(13439..13909)
                     /locus_tag="PKU88_04595"
     CDS             complement(13439..13909)
                     /locus_tag="PKU88_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPX68595.1"
                     /translation="MAELQFVVFKLGKEEYGVNIMQVQEIGHYSEPVKVPNTPEFVEG
                     IMNLRDNVIPVISLRKRFNIPEENLNGPERDLNENRRTIIINLGDRQIAFIVDDASEV
                     LTLDEAGIQETPDIIAGVDRKYISGIGKKGNRLIIMLDLNFLFDEQEQAQLAAI"
     gene            complement(13961..14191)
                     /locus_tag="PKU88_04600"
     CDS             complement(13961..14191)
                     /locus_tag="PKU88_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HPX68596.1"
                     /translation="MISREMIDRINHLSKKSKEAGLTEEERQEQQELRRKYIEYIKGQ
                     VRHGLDSIKFAEDRHDCGHEHHDHCGCKCKKQ"
     gene            complement(14199..14843)
                     /locus_tag="PKU88_04605"
     CDS             complement(14199..14843)
                     /locus_tag="PKU88_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPX68597.1"
                     /translation="MSLLMDMTLRDYSDVLASSEPAPGGGSTAALSGVLGAALTMMVV
                     NLSVGKKNYENLNEKIKAKFMEEAENVKALQKELSGLVDEDTKAFNKFMEAVKLPKDT
                     EEQNQLRGKKMQEASLYALQVPIKTAECCFRLLENQGNIARYGNRNAVSDVGVGALLT
                     LSGLEGAILNVNINLSGIEDEAVRSEAYDKCRRLSAEGRKLHSEILDIVNLRIG"
     gene            complement(14860..16098)
                     /gene="hutI"
                     /locus_tag="PKU88_04610"
     CDS             complement(14860..16098)
                     /gene="hutI"
                     /locus_tag="PKU88_04610"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPX68598.1"
                     /translation="MKADLIINASTIVCCTEGEGAGVFENGYVAVKDGKIISLGEGNA
                     PGDIKGSGTRIIDAAGKTVTPGLIDAHTHLVHGGSREKELPLKLKGVPYLEILKMGGG
                     ILSTVRNTRKASKEELKAKSGKSLDQMLLHGTTTVEAKSGYGLDFDTEIKCLEVAAEL
                     NDSHPVDIVSTYMGAHAVPEEFKGNTQGYIDFMADKVMPYVRENNLAEFMDVFCEEGV
                     FSPGESRAIMQKGKEQGFKLKIHADEIVPLQGAELAGELGAVSAEHLLAATEAGIAAM
                     AEAGVTAVLLPGTSFYLMLGKYAQARKMMDRGVRVALATDYNPGTCPTENLQSIMTFA
                     CFGMKMTPEEIIKGMTLNAAYALDRAGETGSLEVGKKADIVVFDAPNLEYIVYHFGIN
                     HVDTVIKDGKTVVENGRILK"
     gene            complement(16104..17630)
                     /gene="hutH"
                     /locus_tag="PKU88_04615"
     CDS             complement(16104..17630)
                     /gene="hutH"
                     /locus_tag="PKU88_04615"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPX68599.1"
                     /translation="MGHLSIDGEHLTIKDVVDVARNSRRVGLSEQAVERIRKSRDYVD
                     SLVDKEAVVYGITTGFGKFSDVFISKEDTRALQRNLIVSHACSLGNPLTEETVRAIML
                     LRANALAKGFSGIRLETVNTLVEMLNKGVHPVIPEKGSLGASGDLAPLANMVLVMLGE
                     GEAYYKGVGMTGKEAMEKAGIKTVELTSKEGLALINGTPVMTGIGALAVYDAEDLLKN
                     ADIIAAMTLEALKGITTAFDRKVHEVRGQKGQMAAARNMLRIVSGSELTTTQGQIRVQ
                     DAYTLRCIPQIHGASRDAVQYVKEKVSVELNAATDNPLVFADDDQVISGGNFHGQPIA
                     LAMDFLGIAVSELANVSERRLERLVNPQLNDLPAFLTRKGGLNSGFMITQYAAASIVS
                     ENKILAHPASVDSIPSSANQEDHVSMGTTAARKARVILDNVQKVLGIEAFAASQAISF
                     RGDVRLGKGTSEAYKLIRKEIAPVEEDRIMYTDMNKLDEMVKSEALIRAVESAVGELE
                     "
     gene            complement(17712..18215)
                     /locus_tag="PKU88_04620"
     CDS             complement(17712..18215)
                     /locus_tag="PKU88_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPX68600.1"
                     /translation="MKAILERRSIRKYTGEPVSDADLKDLLKAAMAAPSAGNGQPWEF
                     IIVNDRQLLTEITKVHPYSAMLLQAPLAIIVCGKLDNERYKGYWAQDCSAATENILIA
                     AQSKGLGTVWLGVYPVEERVNGIRKIFKIPENMIPLNVIAVGHPAEKKKPSDRYDENR
                     IHFNKWE"
     gene            complement(18240..20090)
                     /locus_tag="PKU88_04625"
     CDS             complement(18240..20090)
                     /locus_tag="PKU88_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPX68601.1"
                     /translation="MISRDDFLPISKEDMQRKKIEQLDFIFVSGDAYVDHPSFGSAVI
                     TRLLESRGYKVGIIPQPDWRNADAFKALGRPRLAFLVSSGNMDSMVNHYTAGKKPRRE
                     DLYSPAGVRGKRPDRAVTVYCNRIREAFKDVPVIIGGIEASLRRFAHYDFWDDQVRRS
                     ILFDSRADLLIYGMGEKPVIQVAEALESGMEIKYITYINGTAYIAPELGNIPDATVIP
                     SFDEVRDSKRKYARAFMLQYREQDYHTGRVLIQPHGERFLVQNPPAAPLTETELDDVY
                     SLPFTRQYHPVYKNQGGIPALKEVKFSLTSQRGCFGGCSFCALNFHQGRVVQSRSHES
                     IIEEAELLLKDPEFKGYIHDVGGPTANFRHAACDKQLNAGVCKNRQCLYPTPCKNLKV
                     DHKDYVALLKKLRSLPGVKKVFVRSGLRYDYIIADKKDEFLRELCENHISGQLKVAPE
                     HVSDKVLRYMGKPSKEVYGRFVKKYFDMNKRLKKEQYLVPYLMSGHPGSDLSAAVELA
                     EYVRDMGYNPEQIQEFYPTPGTLSACMYYTGLDPRTMKPVYVPRDPEEKAMQRALIQY
                     RNPENYGLVYKALKSAGREDLIGFGEKCLIKPPRNPEKRRNPRRENRKRR"
     gene            complement(20313..20864)
                     /gene="thpR"
                     /locus_tag="PKU88_04630"
     CDS             complement(20313..20864)
                     /gene="thpR"
                     /locus_tag="PKU88_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPX68602.1"
                     /translation="MRTFIGADFSRDIKDSIAGVQHIVRENSERGRFKYVGNFHLTLK
                     FLGEIEQAKAPDIGRTLKEIALKHRRIRLNIDKIGYFDGKGSIHALWLGFSGELDKLS
                     SLYSDIDEGMYKLGIKKEIKAYTPHITIAQDLVLKIPFEKLEEKLNFQNFRTVEIREI
                     SLIKSEEAGGKRIYTPISTFQLK"
     gene            complement(20891..21667)
                     /locus_tag="PKU88_04635"
     CDS             complement(20891..21667)
                     /locus_tag="PKU88_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPX68603.1"
                     /translation="MIHVGTSGYSYKDWIGTFYPAGAKAGDLLGIYSRSFDFTEINST
                     YYKIPNSFMFYNMQKKTGDNFIFTVKLHQSMTHGRDAGDKDYEEFAGAVDVLKQAGKL
                     GCLVAQFPYSFHFNRENMDYIQGLRERFADFDLMVEFRNNRWVNDETMEFLRANQLGY
                     ICVDEPAVSGLLDPRAMRTSEISYIRFHGRNDAKWWDHKEAYERYDYLYKERELEQWK
                     DKILELDKQSQNCFVSFNNHFRAQAVINGIMLKKLLGMME"
     gene            complement(21788..22057)
                     /locus_tag="PKU88_04640"
     CDS             complement(21788..22057)
                     /locus_tag="PKU88_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HPX68604.1"
                     /translation="MNKVGIEKPLSNVGRYLESRDCAVEVLEDDTKESPGNLNKFDAI
                     VVTGADSNFMGMEDVMTTTKVINAEGKTEDEIYSEILRAADLKKR"
     gene            22349..23296
                     /locus_tag="PKU88_04645"
     CDS             22349..23296
                     /locus_tag="PKU88_04645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX68605.1"
                     /translation="MKKALVKNKINYTTFMFIPESQSNVKSFRTPAWFPGFVSISIAL
                     LIITSGISFYALSALKLQYDASRKEISKLTTINASQKIEIGNLQRSSEEVQKQLAENS
                     RMLDEVKKAVGINPSPEEGIEIKLVSPEISSPVSLLTEKSDQDMSLKLASIEADFASL
                     TDKIAIQKKTIADSIDPIKKQLAYLMAKPSVKPVAASITASFGYRKNPFTSRGSEFHK
                     GIDFAASYGQTVSATGDGIVSFAGWNAGYGRMVIISHGYGLTTLYAHNSKLLVKQGDK
                     VKKGQAIAKVGNTGRSTGTHLHYEVKLNGKNVNPANYFN"
     gene            23334..23693
                     /locus_tag="PKU88_04650"
     CDS             23334..23693
                     /locus_tag="PKU88_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPX68606.1"
                     /translation="MFGSGKKSINSERVDTIIGRGTIFEGTVNADGTVRLDGKVLGGL
                     NISGNLIVGEEGAVKGNVKTENAFVAGVIEGNVIATSQLHITHTAKLVGDITVKNIII
                     DEGAIFIGNCKIITDNT"
     gene            complement(23752..24084)
                     /locus_tag="PKU88_04655"
     CDS             complement(23752..24084)
                     /locus_tag="PKU88_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68607.1"
                     /translation="MANGIGYGGNFNGNFFDLLSDLIGETVTIFTTSGGQSGCGFTGV
                     ILAVNQCFVRLVTHIGPAPGCALGNACHSPCCPTIGCSGGFSNVRSVGSVTDIPLDRI
                     VAVVSNAV"
     gene            complement(24232..24549)
                     /locus_tag="PKU88_04660"
     CDS             complement(24232..24549)
                     /locus_tag="PKU88_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68608.1"
                     /translation="MVDGVAGNAFGGGNFDLLQDSIGETVTIFTTSGGLSGCGFTGVV
                     LAVNPCFVRLITHIGAAPNCALGSCCGVPAGGYGSYGNVRTVGSVTDIPLDRIAAVVR
                     NAI"
     gene            complement(24580..24816)
                     /locus_tag="PKU88_04665"
     CDS             complement(24580..24816)
                     /locus_tag="PKU88_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68609.1"
                     /translation="MANGLGSTGCGCGAPASVSPILDKCKGNGFLLLLLLFVLLASCT
                     TLGREKNFFFIIILAIIAFGGFGRTFGFNNGTAI"
     assembly_gap    25081..25180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25200..25433)
                     /locus_tag="PKU88_04670"
     CDS             complement(25200..25433)
                     /locus_tag="PKU88_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68610.1"
                     /translation="MANGLGSTGCGCGAPTAVSPIMDLCKNNGFLLLLLLFVLLASCT
                     TLGREKNFIFIIVLAIIAFGGFGRTFGFGGNDY"
     gene            complement(25467..25736)
                     /locus_tag="PKU88_04675"
     CDS             complement(25467..25736)
                     /locus_tag="PKU88_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68611.1"
                     /translation="MASGFGVANPVTAAGCGASPAPVGGFFGFSNAWIWIILLLIFVL
                     PRLGFKGCTGAGGLFGGFSWIWIIILIIFLVPGILPGFRLGGFAI"
     gene            26170..26433
                     /locus_tag="PKU88_04680"
     CDS             26170..26433
                     /locus_tag="PKU88_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68612.1"
                     /translation="MKMLRKPVEAIVLFDLEGNPVPVRFRYLDDRSELITVKVDRVIK
                     RELDKFAGNRMLKFTCQTRIGNQIKPFELRFEIDTSKWYLYKI"
     gene            complement(26441..27688)
                     /locus_tag="PKU88_04685"
     CDS             complement(26441..27688)
                     /locus_tag="PKU88_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPX68613.1"
                     /translation="MRTIMHIDVNSAFLSWQAVYNLQKGDTLDLREIPSVVGGSQADR
                     RGIVLAKSIPAKKFGITTGEVLWQARQKCPGLVVVPPDYNLYMKCSNILYETLKEYSP
                     GLQRFSIDELFLDYTGMEPHFGDSVAAAHMIKERVKKELGFTVNIGIGANKLTAKVAG
                     DLKKPDMVHTLFPDEIESKMWPLHVGDLFMVGRQTRKKLVEMNITTIGDLARADRRFL
                     RDRLKSFGNLIWCYANGIDESPVYPGYYLQMKGIGNSTTIKFDVTERQIAHKVLLSLT
                     ESVSLRLRAARACCRVVSVEIRTAELNFYSHQRKLMAHTNVTMDIFETVRELFDECWG
                     GEKIRHLGVRVTDLCSDEFMQASIFDQQCKYKKQALDSAMDVIRGRYGNDSVMRAIFA
                     DGEFAPMSGGSGGEDYPVMSSIL"
     gene            complement(27826..28338)
                     /locus_tag="PKU88_04690"
     CDS             complement(27826..28338)
                     /locus_tag="PKU88_04690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX68614.1"
                     /translation="MKNMIMEMGGLMFCLTGSEYLDLILDIEKANSDFVFVWPKERHM
                     KAMNSEDEMHISVFSSAERKLVGYILLSGIGGEDSAVEFRRMVISDKGKGYGRTSVKF
                     IKKYCFEVLKCHRLWLDVFTDNEKAIGLYRSEGFVREGILRDCKKHGDSYRSMVIMSM
                     LEQEYIPCSF"
     gene            complement(28557..30227)
                     /gene="gltX"
                     /locus_tag="PKU88_04695"
     CDS             complement(28557..30227)
                     /gene="gltX"
                     /locus_tag="PKU88_04695"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPX68615.1"
                     /translation="MHNLTQNRELADLLFPDIQKTPEYYEMLYPKRQLPEGAKVTRFA
                     PSPTGYVHMGSLYASLVSERLAHQSNGVFFLRVEDTDKKREVEGGVSNIIRALSRFGI
                     SFDEGVTDVDDEKGSYGPYKQSERMKIYKTFAKRLVEKGLAYPCFCSEEDLDKIRAQQ
                     EEKKETPGYYGEYAVHRNSTLEQVKEELSRGKPFVLRVKAPDNPDSRFVFKDLIKGDV
                     EMPENFQDIVLLKSDGLPTYHFAHVVDDHLMRTTHVTRGDEWLPSAPLHVQLFEMLEW
                     EKPYFAHISPIMKMEGSSKRKLSKRKDPEADVSFYHEQGFPGISVIEYLANLINSNFE
                     DWRRENPESPNTSFKIELERMSVSGAMFDIVKLTDMSKDIVAGMTAEEVYNMSLEWAE
                     EYDSELWALLSRDEKYAKRIFSIDRGGEKPRKDIAKWLDVKPNIFYFFDELFTRDAAE
                     GYNFPDTIPADEVNRLIREYAKVYNHSDSKDMWFDRVKGFCEAMGYSKDVKTFKKNPE
                     AYKGHVGDVTGIIRVAVTNRRNTPDLYEIMQVMGEKMVMERFDKAINI"
     gene            complement(30261..31574)
                     /locus_tag="PKU88_04700"
     CDS             complement(30261..31574)
                     /locus_tag="PKU88_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPX68616.1"
                     /translation="MDILLKNAQIITLNDNDDVIPEGSIGIRDNRIDFIGESNGCIEA
                     EYTKVIDCKGRTVMPGFVNAHNHLAMTMFRNYADDMKLMDWLFTKIFPLEDKQNDESV
                     YWGSLLAMVEMIKSGTTTFADMYFFMESTARAAAESGMRAVLSRGLQGESGEEEPDYR
                     LKESDELFRKFHNSYNGRIKVMLAPHSVYTCSEAYLRKVAAKSLEQGVPVQIHLSETK
                     EEVSTCIEKFGRSPVKYLEDLGLLNDRTVAAHCVAVDDDDIGILASRRVNVVHNPGSN
                     MKLASGIAPVAKMLEKGINVCLGTDGASSNNNLDMLEEMRLAAYLQKIYTNDPTALPA
                     EAVIRMATLKGAKALGFENLGSIEAGKAADLIVLNTERAHYYPKHNIKSAIVYSGNSA
                     DVETVIIDGNLVMESGHLVTLDEERILYKAQKWASKLTGQECPQP"
     gene            complement(31578..32828)
                     /locus_tag="PKU88_04705"
     CDS             complement(31578..32828)
                     /locus_tag="PKU88_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPX68617.1"
                     /translation="MRESIIRDISSAESGHRKIEWVKGHMPVLSAIEKEFIEEQPFAG
                     KKIAMSIHLEAKTAYLSKVLKAGGAEVSVTGSNPLSTQDDVAAALAEDGLNVYAWYNA
                     TPEEYNHHLNAALDIGPNIVIDDGGDLVHLLHGERQELLPDILGGSEETTTGVLRLKA
                     REREGLLKFPMISVNDAYCKYLFDNRYGTGQSVWDGIMRTTNLIVAGKNAVVIGYGWC
                     GKGVAMKAKGLGANVTVCEIDPIKAIEAYMDGFQVMSVDEAASIGDFFITVTGCAKVI
                     TEKHYKAMKNGVVLANAGHFDVEIWKPDLETLSCGKKPMRNNIMGYVMEDGRIINLLA
                     EGRLVNLAAGDGHPAEIMDMSFALQALTARYISLNKEKLENRVYKVPEEIDRKVAYMK
                     LKALGIRIDKLTKEQEEYLRGWGE"
     gene            complement(32886..35297)
                     /locus_tag="PKU88_04710"
     CDS             complement(32886..35297)
                     /locus_tag="PKU88_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX68618.1"
                     /translation="MSRFRELAPEELSNKIDCGLFEFETTADLDPLNGIIGQSRAAAA
                     MEFGLAIKRPGYNIYVSGITGTGRSSYTRSIVSRIAANESIPNDWCYVYNFKNPDKPK
                     CINLPAGMGYKLQKDMKRLLKDLKIKVPQAFEGEEYEKQKGQIIQEYQAKSSEYMESF
                     NVFAREQGFVIRKSEHGLMTIPLRDGKPMDDKDYIEMPPEERKKIEDNSFMVQGKLME
                     TLKRMKEIEKAARDKIDQLETKIALLAVEQPIMELKEKYDKYKNIIEYLQAVQRDIIH
                     NVEDFRNLEVGKAETLDLVERIREKDFTIKYQVNLAVDNRDTKGAPVILESNPKYDNL
                     LGKIEYESNMGVVTTDFTKIKAGSLHQANGGYIIINMSDLMRNPESWEGIKRSIKTGN
                     ITIENIFSMSGIVSGGIKPEPIPLNVKIILIGSSYVYQILYTHDEDFRKLFKIKADFD
                     TDMSSDKGNCLKYASFIRSHCEENGLMHFDRSAVAAVIEYSRRLAEHKYKLSTRFNDI
                     IEVLYEADAWARIAGEQKVTGSSVKRAIEQKWYRSSRIEEKMQELIDTGVVLISTEEK
                     VVGQVNGLSVMNFGDYTFGKPSRITAAAYAGEKGIVNIEREVEMSGSIHDKGVLILTG
                     YLGDKFAQDFPLTISASICFEQLYEGVEGDSASSTELYALLSSLSELPVKQYIAVTGS
                     VNQKGEIQPIGGVNEKIEGFFQTCKTRGLKGNEGVMIPWQNVDNLMLKDEVIEAVREG
                     MFHIYPVKKIEEGIEILTDVPAGTRDESGNYPENTVFHLVQKKIEKFARIAEEFDDCD
                     QERRV"
     gene            complement(35336..>36063)
                     /locus_tag="PKU88_04715"
     CDS             complement(35336..>36063)
                     /locus_tag="PKU88_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPX68619.1"
                     /translation="VEVEVVGIKIDNVNMDEAAALMEEFLETDRCSKVFTPNSEILLA
                     AVGDGEFGSVLNSGHLVVPDGIGVVIASRFYGTPLKERVAGFDLMMRLMDIADSRGKS
                     VYLLGGREGVAEEAAIKLTESYGSLRIAGIRNGYFEEDSENDIVNDINNSKADILLAA
                     LGAPKQEKFIYKHGDNLKVKIAMGVGGSLDVLAGKVKRAPEFYQKAGLEWFYRLVKEP
                     RRITRVLRIPKFIALAFYDAKVK"
     assembly_gap    36064..36163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36164..36846
                     /locus_tag="PKU88_04720"
     CDS             <36164..36846
                     /locus_tag="PKU88_04720"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPX68620.1"
                     /translation="DTANFLNALGANIKGAGTDVIKIKGVESLGGCEYSVIPDQMEAG
                     TYMIATAATRGDVLIKNIIPTHLDAVSAKLREMGVEITEYEDSVRVKAPSRTKAVNVK
                     TMPYPGFPTDMQQPMSVLLSVSEGTGIVTESIFESRFKYFDELKRMGAKIKIEGRVAV
                     IEGVSKLTGAPVYATDLRAGAAMVVAGLTAEGKTTISNISYIDRGYERLEEKLINLGA
                     KIRRIEEK"
     gene            36858..38540
                     /locus_tag="PKU88_04725"
     CDS             36858..38540
                     /locus_tag="PKU88_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPX68621.1"
                     /translation="MKLKQSLSILLVFCFFIVILMPGTAAAAASGTKIPGAFWSLVTP
                     YQNAVDANDLNNIIVYGNKIIELFNGMEETQQVLEIITPRLDQVAKAYEALGRFDLAV
                     EYFSKYIPRAEKLGWKDGVTYARSKINTLGFDIELYAKTTNLEKSVYFGSKYEPRSGV
                     YFGSNYDTDPRIGTYSWNDIKNYFPRKNTAYLTYLHWNEDIKSFDRYFKDAKANNIAV
                     QLAWNIEDSEIDNVLKSIKSYDSYIRATADYLGKLNIPVFLRFAGEMNIKDNCKDYNS
                     FIAAFRHVSGIMKAKAPNVAMIWSPNDISAKGRTYDQYYPGDEYVDWIGISTYTQRYF
                     QGKKDWGSLQDTNDSVYMTGNYANPLAKIKPIMDAYGIKKPIMISETGVSHYAKLAGE
                     DLSPWAKVQLRRLYTYGPMIYPELKGIYYFNANNDIITKYDDYSLYGNSAFNELYNEL
                     VNSGNFISSVNGNPAFRYEEVTDYSTPGLKLELSTYTVVPKVLNPTVQYKLDGKIHAV
                     RTVIPYSIELDLSAVPKGGHKLTVEIYDGNNKMGSREYTLTINDDNANLKGN"
     gene            38567..39694
                     /locus_tag="PKU88_04730"
     CDS             38567..39694
                     /locus_tag="PKU88_04730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX68622.1"
                     /translation="MRNYNILITTMRLDIGGAETHIVELAKGLSREGFNVIAASAGGV
                     YEKELAESGIKVYHVPLDNKKPHNVIKAYFKLKNIIKENRIDLVHAHARIPGFICGLL
                     HKRMGFPFVTTAHGTWKTGYGLRYITNWGQKTVAVSEDIRQYLLDNYKIEDKDIKVTI
                     NGIDTDKFSSGIDCSDIMDELNLKPEDMKTVYISRFDSDRSYIINKLLEVIPELSESI
                     PSLKVIMVGSGNILDSIKEKAREINNITGQNTIIVTGSRTDINKFTALADLFIGFGRS
                     SLEALSAGKPLILAGNEGYIGILNENSLDDAIKSNFSGRGKSKADSKSFKDDILKVLS
                     LSGEERKALGDFGRSVIENSYSVRKMVQDNMEVYAQLLEHK"
     gene            39708..40799
                     /gene="csaB"
                     /locus_tag="PKU88_04735"
     CDS             39708..40799
                     /gene="csaB"
                     /locus_tag="PKU88_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HPX68623.1"
                     /translation="MYDVMISGYYGFGNSGDDAILLAIIENLRKIKKDIRIVVLSKNP
                     KDTASIYGVDSIDRFNLFKVIGTMKKTRLFLNGGGNLIQDVTSTRSLVYYLTTIYLAK
                     LMGLKVMLYANGIGPVGRRTNRYFTSKIIDRVDIITLREEASGLELKSLGVKKPEIRV
                     TADPALGLEPAGSEETEKLLQKEGIPADTPLIGFSVRKWSGYEAYSRILAQAADYIEE
                     TYNAKSVFIPLHFPSDLAAAEDIASKMKHAPFILRNIYETDKILGIMKKMDLVLGMRL
                     HALIYSVSLSVPVIGLIYDPKVQGFLEYVNQPWAGNVADLNLDSLKKLIDEVWSSRKE
                     ISQQLEKGNRLLKQKAYENAEIAIKLLEQ"
     gene            complement(40823..41329)
                     /locus_tag="PKU88_04740"
     CDS             complement(40823..41329)
                     /locus_tag="PKU88_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HPX68624.1"
                     /translation="MLLDEKGRLFGKISIVDIGVLLLIAVLIGGVYYKFFMVDKDQNA
                     AKFDTIEYKVLVEEVRQQSVDAIEIGADIYDVKTNSPMGSITDIEVLPATDQLTKADG
                     TMVIAEKPERFNVLITIRVPGIETKYGFRANGRLDINRESQQVMDTRMIEMVAKIVDV
                     KNLSKAQQ"
     gene            complement(41342..41848)
                     /locus_tag="PKU88_04745"
     CDS             complement(41342..41848)
                     /locus_tag="PKU88_04745"
                     /inference="COORDINATES: protein motif:HMM:NF025582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HPX68625.1"
                     /translation="MKDMALWDDRGRLFGKINIMDLIIILLIILTAAGGYMFFSGGNS
                     RQAVNTGKVVYDFEITNVNKDFVDAITPGDPIRDSTRGNELGTVVSKSSRDAAMLNED
                     LINGRYIIAEVPDAYDVVITIEADANITPASIIIGGAEVKVGKKFFIKGKGYANQGFV
                     TKMTLVGQ"
     gene            complement(41850..42959)
                     /locus_tag="PKU88_04750"
     CDS             complement(41850..42959)
                     /locus_tag="PKU88_04750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX68626.1"
                     /translation="MIKVMHVLSDMKIGGAGSWLLNLLGAIDKEKFEIKVVLPRGSML
                     TDKIKNLGFEVIAIDGIKDKSYDARAVNLLYYIFRAEKPDIVHTHASLSARLAGKRAG
                     ARIINTKHCIDSPITGIRRFAGALINDRYSDGIIAVSEAVKRNIAENGVPDDKISVIY
                     GGINPVKVLDDKEKSLARGKWGIGEEEVVVGIVARLAEVKGHKYFIDAAEIISRDNDN
                     VRFFIAGIGPKEEELKELIKQKGLTDKVVFTGFVDEIYEIFNIIDINVISSLSEALCL
                     SLIEGMSVGKPSVAADTGGIPEVVKDGYNGFLVPPGDAERLADAILKLIHDPELRKTM
                     GDRGREIMEKSFTAEIMAVKIEELYESVARKNRRE"
     gene            complement(42956..44872)
                     /locus_tag="PKU88_04755"
     CDS             complement(42956..44872)
                     /locus_tag="PKU88_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPX68627.1"
                     /translation="MKESLIFGMILKVYDRIVFYYENSMFFIVMQRLGHGFGKLAASS
                     SILNFFDREWNIADTWRQSLVFRILVSPLRWFRFASQKFSESVNVAIEESRVLKAIRV
                     FPENLFDMSTRVYGLLFITFSAVFGLLDFTSRNAGMLFGMSEIMKLALFLGGAVLILI
                     NRPVKALAEGSIVSKAVFGFFNVSHDAFYDKRTEDAVLNKRFEITAIVTGTALGAMWH
                     FLQPMAFALAVGGIAALVLVLWRYEIGVFAVAGFIPLAPTMALLGLILLTAVSFFIRL
                     FRDKSMKLRSTILDYFVILFGMVLLYSFITSYTPGSSMLALLIHMAFIVFYFILVSTI
                     KTRRQLYFLAALLVLSTTIASLYGLYQLKTVGATSEAWVDTTLFEDIKARVGSTFENP
                     NVLGEYLVLIIPIAIAMLWGQKNWLSKLAILGLTAIMLGCLVYTYSRGAYVGLMLAFA
                     LFAVLRDRRFVILGVVGLLMLPFVLPASVINRFASIGNLNDTSSFYRISVWLGSLKLA
                     QDYWLSGIGLGLEPFKLVYPKYSLNAAYAHHSHNIYIQLMIETGIAGLLMFFAIIAVY
                     YKTLLAGFYKTKDKFISTFMIAVASGMAGYLAQGMVENIWYNNRVLLTFWVMLAFGVI
                     TKALITEDNEVSGI"
     gene            complement(45106..45654)
                     /locus_tag="PKU88_04760"
     CDS             complement(45106..45654)
                     /locus_tag="PKU88_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68628.1"
                     /translation="MVNSKSRKTSIIVLTAIILVLSAVVISAADGVTPGSEQDPIVTQ
                     SYVEQKSEQIKYYIDSLIAQETAKLKTELELKEQEIAKLREEIKDAAKAAGKFQVVEM
                     QKGQMLIAGEGAEIIPRSGKFSAIEDAFGGLSDITAAKDLKNGEAVVNNHMLIAARGE
                     GRGLKALADKSFLIIKGTYSLQ"
     gene            complement(45936..47099)
                     /locus_tag="PKU88_04765"
     CDS             complement(45936..47099)
                     /locus_tag="PKU88_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPX68629.1"
                     /translation="MSKEKILKTYARFDVTAVSGKGCLIYDINGKEYLDFVSGVAVNC
                     LGHCHPAIINAIREQSCKLMHVSNLYWNELQMELADRLCELSGLEGAFFCNSGTEAIE
                     AAIKFARKYGKLKGGALKHEIIYLNNSFHGRTLGALSVTGQEKYQKDYMPLIPGVKSV
                     DLNDAGQLRKEMNSNVCAIIAEPVQGEGGILAADKSFLEEAKKLCEKYDSLLIFDEVQ
                     CGIGRLGSLFAFEKFGVKPDLVCIAKGLGGGFPIGAVVASGKAAECIAYGDHGSTFGG
                     NPLACKVALTILEELLQNGVLENVEHMSKYFVGKLQALKEKHGSIKSIRGIGLLLGLQ
                     LSIDTKKFIDECIDSGLLLVGAGADVVRLLPPLNIQEEYIDRAIDIIDSVLSK"
     gene            complement(47092..47919)
                     /gene="argB"
                     /locus_tag="PKU88_04770"
     CDS             complement(47092..47919)
                     /gene="argB"
                     /locus_tag="PKU88_04770"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPX68630.1"
                     /translation="MDLGGTVVIKYGGSITKDKEATDAFLEDAAILRNAGVKVVIVHG
                     GGPDINRMLGRLNIAGEFVKGLRKTNEAAMEVVEMVLSGRVNKELTSKLIGKGADAVG
                     ISGRDGKLLEAERMYLNDNGSNIDIGYVGEVTKVNGKILTDLLSLGYVPVISPVAEGK
                     EGKAYNINADYAAAAIGASIGADSLVYLSDVDGLFGDIGNPDSLISSITTAEIEKLVQ
                     AGAISGGMIPKMKCCIEAIEKGVKSVCLIGGKKPHILLELFHGKEAGTIIIGGEENV"
     gene            48069..49106
                     /gene="argC"
                     /locus_tag="PKU88_04775"
     CDS             48069..49106
                     /gene="argC"
                     /locus_tag="PKU88_04775"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPX68631.1"
                     /translation="MINAGVVGSTGYAGYQLSMILSQHKNVSIVFLSSHNYSNLRFSD
                     VYGNLKYLLDNMCIDMQDIESKLSKIDVLFLALPHGQSFDLVEKALGLGVKVIDIGAD
                     YRLRDQKVYETWYGVTHKLPGRLKDAVYGLPELYRDSISNSSLVANPGCYPTASILAL
                     APLVHTGIIDNTSIIIDAKSGVSGAGRSANVSSLFCECGESVKAYNIATHRHTPEIEQ
                     QLSDAAGAKITLSFTPHLVPMSRGILATCYGSLKKDISQSELYDLYRSYYKNENFIRI
                     TDSLPETRFVKGSNICDIAVRVDERTNRVIAISAIDNLIKGAAGQAVQNMNILFGLYE
                     HTGLDMLPNML"
     gene            49121..50338
                     /gene="argJ"
                     /locus_tag="PKU88_04780"
     CDS             49121..50338
                     /gene="argJ"
                     /locus_tag="PKU88_04780"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPX68632.1"
                     /translation="MKIIKDMTITDVPYFKASGVSCGIKKNGNKDLCVIYSEKDSVAA
                     AVFTTNKVKAAPVILDMKHIQSKSIRAIVANSGNANACTGKQGQKDANTMAELTASLL
                     NIPSDSVLVASTGVIGVPLPMKNIEAGIRNACRNLSYDGGKDALRAIMTTDTCEKSVT
                     VEVELEGKKVLLSGIAKGSGMIHPNMATMLGFITTDANISKEMLQEALSESVKKTYNM
                     ISVDGDTSTNDMVIMLANGAADNKLIGARDKNFDAFMKALDLLNTELAKKIARDGEGA
                     TKLIECTVSNAVSYADACILSKSVIGSNLTKAAFFGNDANWGRILCALGYSGGNFSPD
                     KADIYFESEAGCIQLVADGEPLQFCEETASAIMDKEYVIIKVDLKEGGYSATAWGCDL
                     TYDYVKINGSYRS"
BASE COUNT        13398 a        12526 c         9397 g        14953 t
ORIGIN      
        1 gtcgcattat tcttcttatg cgctaaatga aaaagcctga agaggtcaat gacttcttca
       61 ggcttaaatg ttgtatttct acacttctac atttctggtc tttctataag gatgaaacaa
      121 agatgtcacc acttcaagat ccctgttgat tacagcagtg gcatgatgca ggaaatcttt
      181 tgaaatgtgg tatctgaggt ctataaattc aaattcataa ttgccttcct tcttttcaca
      241 ctcaacgtga aataccgggg taaacttttc aaaaaacata cccacaggag tatccatggc
      301 aattttgtat aaatctgtgt caatgttttt cttattgtct attatcctga accttctggg
      361 aaatatatta acttccccga ttatctttct tcttcctgta tacaatacaa aatgccattt
      421 tataattcct atcatagaag gcattattct gaaatactcg cttttgtatt tatagccgag
      481 attctgataa gcaatatcct gtgccagctt tttcataatg cggcgaagta ttaaatagcc
      541 ggcaaaaata aatactgtca tataaggcgg aataataccc cttgtgtaag gcagaatatt
      601 aatcaagcac aatattataa gaatagggtc gtataccaac agcaaatcaa aggttatatg
      661 ctttttgctc aaaggccata atatctgtgc tccatatgaa tttgtgatat ccagggcaat
      721 atgggaaaaa cagcccagca aggcaagggg aaaaatattc caaaatgctg caccgttgaa
      781 agccagcatc aataatccgg ttattatgcc tgaataaaca aacatcattg gtattgaatg
      841 ggacatccct ctatggtttt tcaaatatgc atagtcgccc ttaagctgca ttaatatatc
      901 gccgtcagga attattgcgc ccgctacaca agctatcatt gcaggatttg aagctgccaa
      961 aggttctccg gacattactg cgatagtaag tcctactgct gcgtgggtta ctggatccat
     1021 taataattgt ccccctaaat acattgataa gtatacataa aatataaatc acagggttca
     1081 ttatatcata atatttattc ttttcaaata aaaatttaaa gaaaattgta aaccctccgc
     1141 agtatgcaaa ggcaaatatt cgtattgatt tttaaacata tgtatgctat catgttttat
     1201 gtgacaaatg ccggagtggc ggaattggca gacgcacatg actcaaaatc atgcgccctc
     1261 aaagcatgtc ggttcgagtc cgaccttcgg caccatattg agaataaaag gtttacaaag
     1321 aaaactttgt aaaccttaat tattttttgt tcttacttca atctgctttt tcttaacagc
     1381 attaattaac agcagatgct gttcaatacc atattattcc tcgtaaacta ttacagcttc
     1441 cttggtgctg tttatccaat ccactttgca attcagattc tcagccgcaa atcttacagg
     1501 tacaaaggtt ctgtcatttt ttgaaacagg tgctatatcc atttttttgc tttctccgtt
     1561 tatttttata tctgttttgc ccaaccacat ttcaacagta tttcctcttg ctgtcagggt
     1621 tattttcttt gtggctccat cccactccac tgtaccgcct atagcctcta caaccgctct
     1681 tataggcacc atggtcctcc cagctatcac aacaggtgct gttcctcttc ccggatcaac
     1741 ttcctgactt actccgtcaa cgctcatatt gggatcttca agttttaaca taataaaatg
     1801 cttgtagctt ccgggcttat atacctgatc ccccgttgtt acaacaaacg ttgcaattac
     1861 atccccaagc ttttcatcga ttccttcgaa aggcttggct tctgccaata ccggctttac
     1921 tgaataatag taagttgttt tgggctccac atttacatct gcataggagg tacttgtgat
     1981 ataaaagtct gtaacggata tcccaagctc atttggggta gttgatctaa agagtctgta
     2041 tcccaatccg ttagcgggct gccacataat cctcgctcct gattcaaagg gttcggcgcc
     2101 gcttcccgcc gaaaccggtg ttgcaggaac gacaggaaca gcaggtaccg ctccttcttt
     2161 ccactcgtag atataggtat agccatagcc gttgcccaaa gtcaggataa cagctttttt
     2221 atcccccgaa cgaccctcgg gaatcacctt ctcgctttcg aagtagcctt gcttatcact
     2281 attaatatac tttgtcccat cttccaattg aaaagatata taggatgatg tagcaccccc
     2341 gggctttatg cttgccgcat ccaaatgagc atttatctgt actccgcccc aggacttgga
     2401 actgactacc ttataatcaa tgttgaatcc ggttttctga ccggaggcaa tataggaggg
     2461 aggggtgtcc caaacaacct gataagttac gtgggcaatg gggttcccat tatcatatct
     2521 tcgtgaatga gtaattgtca tatctccttc tgaaccttca accgcatatt tatcatatat
     2581 gacatccgaa gtccctatta taccgttttg tatttctcct ttgactgtgt aatccaatgt
     2641 cgacaaataa taattcgttt ccacaaggtg ccatcctccg gtggtatcgg catataccgc
     2701 ttcaggtatt aggaaatcta ctgaaaatga aagggttaac agcagagctg ctgttaacaa
     2761 acccgatgca atttttttta tgttcgctct tttcatggaa ttcctccttt acattctaac
     2821 ttttttggaa aactacttcc cgtcggccat acaagaaata ttgctcattc tcctttcaaa
     2881 ttccatgtta gcattatagg ataaaaaaaa gacctaccca accgggtagt tcctgcacaa
     2941 aaaccatcct ttttatttgc ctatcccaat gcccctcttt tccaaaataa tatttacaag
     3001 ttctccgcgg ctttgtattt tgaactttga ataaatattt tttacataat attttaccgt
     3061 attttcactg atataaagct ctacggcaat tatcctgtaa gtctttccct gaagcaaccg
     3121 ggatgcaacc tggttttccc gctccgacag gttctcgaac actgtgttat aatcaggttc
     3181 tctggtctgt tcctgagcag atatttcgga gaatgctttt aagtaggtat gatctctgag
     3241 aagtgaagac agatatttat gcaaaagcgg gagaattgca agtgccacac aaactatggt
     3301 cagtgccagc aatacgggat ctgcgctttg tttatcgaca gatattatag cattccctat
     3361 caacccgccc agcagtatac cgattacatt ggcggagagg cctattccaa gaattgttgc
     3421 ggggttttta tgaaagtcca acatttctcc aagaatactc caccaaaata aatcacatac
     3481 tccaaaggct ccaagcatca atgtattaac tgcaatataa ccggcaacgt tatggtcaag
     3541 gatcataaag cttatgaatg aaatccctat catagcgatt gcaacgtaga gtatataagc
     3601 ccggttcacc ttttgaggca gatgacccat tataaaaaca gcaataatat aaggaattgc
     3661 ccaataccag cttgtcagcc atttaagatg ggcaaaggca ggattaacca cttgatacat
     3721 caaacccgag ttaatagtaa taactataat aaagatacat aaaaaaacaa gcagaggcgc
     3781 tatgcttatc cggcttttat cccgctcaaa ggaagcagaa gacgaaatgc aattatcccc
     3841 ggggagtttc agtgcaaaca ggaatgcaag gatcagcatt atcattgata aagccaatcc
     3901 gctgtgtgga gatatccaga gtgctgccat attcagtaag atcataagaa tatttgacag
     3961 tatcagcata tctgcaacgg tttttatacg cccgattttg ggtgtgctgc ttttcaaata
     4021 ataaccccat gcagccacac agcttccgga cataaatgag cctgcaataa ttcccacagt
     4081 ccataatacc gagggtggaa agaaaaaagg cacggacatg acaatgcaaa agaggtaaga
     4141 atacaggaaa gttctcttgg ctcctctttt tgtttttatg aaaaacccgc ttagcagcag
     4201 ccccaaaaat acagctgcaa ttcctccgaa aaccatagca tgggaatcaa agtcaaagat
     4261 tcccatgaga gagtaaaaaa cttctccttc aaaaataaag gatagcatcc aggatgaaaa
     4321 caatgagtgg gcaataacgg acagccatcg ttcacttaat aatatttttg atctgctatc
     4381 cgtaaatctc atatcattca ccccaataag ttatattttt atgctgtaac aaggtttcaa
     4441 tgatgggttt tacacccttg taaaaacctg tcctctcaac aaaaaacccc ctaaaattgt
     4501 tattaatccc gattattaac aaaggggggc aaaaagttta tatctaaaat attaaccgaa
     4561 agtcctattg actgtgctgt tattcttctg ttttatccgg ttctatatct ctcttctcaa
     4621 ttcttatttt tgatatcctc ttgtcatcta cttcttcaat cctgaaatat atattgtcta
     4681 ttgtcaactc ataccctacc tccggcattt tgcccgagtg gctgaggagc attccgctta
     4741 ttgtatcgaa ctcctcctct ccggatagtt ttgtattcaa tatatcgttt acatcactta
     4801 cattcagcat tccgctgaca atatatgtat tgtcgtcaat tttttcataa tcgagaatta
     4861 catcatcgta ctcatcaaat atgttgccga cgatttcctc aagcaagtcc tctattgtaa
     4921 taattcctgc cgtaccccca tactcatcta tgactacggc aatgtgggtt ttggatttct
     4981 gcatttcctt gaaaagctcg tcaatcttct tgtactcggg tacaaagtgt gcttctctta
     5041 ttatttcttt aagggatacc tcccattcga cattatttct tatcattgca aataaatctt
     5101 ttacatatag aattcctatg atattatcaa ttgattcttc atatacaggg attctggaaa
     5161 agttctcctg cactattaca tcaagtatct cttcacgagt ggcttcggag ttaagagcaa
     5221 caatatcgat tctgggagtc attacttctt ttgcaaccgt atcatcaaac tcaaatatac
     5281 tgttaatcat ttccgtctcg gcttcccgga atatcccttt ctcttccccg acatttatca
     5341 tggaccggat ttcttcttct gttatctgtt cctccgttac cttcgccggc ccccccatga
     5401 gcttaactac taaattagtg gacacggtaa gaaattttac aataggataa gttaacttgg
     5461 acaaaaggtt tattgtgccg gcaaccctaa atgccgtctt ttccgactgt gaaagggcca
     5521 ttcgtttggg gacaagctct cccagtatta aagtaaacag cgcaattata ctggttacaa
     5581 taaatgtgga aatcccgaca gctgaagcag ataggaacgg aatattcaag ctcatgagga
     5641 atccggtcaa tccgtttgtt aacgtctgag caccaatggc actggcaaga aatccggcaa
     5701 gagtaattcc gatctgaatt gtggccagca gcttgctcgg ctcttcaaga aatctgttaa
     5761 ctaaaattgc agctttgttt ccttcatcca caagaggctt gattctggat tgccttactg
     5821 aaattatagc catttcggaa gcagcaaaaa acgcatttaa acctataagc aatattataa
     5881 gcaatatttg cgtccataag tcaccgtcca ttaaaataat tccccctagt aaatcatttg
     5941 ttaatattat actatttatt catatgcatg tacaaattaa atattgactg atatatacct
     6001 cctcttccaa ttttccaact atatctgccc tataatgaga aaggctcctg ataaagcact
     6061 ttatctgccg tggaggcatt gttacaaccc ttaaggtagt aatgcaccat atttgccatc
     6121 gctattcggg caaatccatc aaaaacacca gaagcgtaat aatcagaaag gttttgtcac
     6181 acttattttc aatatacaag ataaaagcaa tggcaaggat taaaaaatcc tccgggctca
     6241 gccgattaag aatacttaaa attaaatgct cggtactcat tttatttccc tgccttgctc
     6301 ataaaatttt caaaaaatgc aaccaggttc ataattttga tgcaatcatc tatgcccctt
     6361 tgcctttttt ctgatacaaa aggccttagc gagtataaaa gtgaaatcct gctatcatct
     6421 tttacccctg caaacatctt atcaaaaagc aagcccaaaa gtgccctttc atcaaaatcc
     6481 cccaggctgt cttttaagtc tgcctggctt tcataaactt ccggctcttt tatctcattt
     6541 tttttttgag ggcccagccc cgaaaggagc atgcccataa gctgtttttt cagaccttcc
     6601 atatcattgt ctttaagcat gtttatgagc tgatcacttg agccgtctgc agacctgtta
     6661 ttggaaagat atttgatgat atcctccttt aatccgtcta catccccctt gttgatttta
     6721 tcttttatgg cagggtctat attactgttc cggcttattt catttaccag catattttta
     6781 acctggtccg gggatacgtt gtcaatatcc atgcctccca gcttttcgga aaccatattt
     6841 ttcagttcat tcatcctgct gtcagacaca ttgttatcct tgccgtgtgc catgttgtga
     6901 cctccaatct ctattgattt attatcataa tattcctttg ttcatcattg gttactttga
     6961 ttaacgtccc actttcatac tatgtacaaa ccgggatttt ggtgacagcg ggtaatgaga
     7021 catggggacg tcagacggga catggggacg tttcgcgtgt ccctctggaa gtgggacagg
     7081 ggaaacgtcc ccatgtcccg cgaaacgtcc ccatgtctca aatccttgcg gatttatgat
     7141 ataataaata ataataaaca aatattaaga atgatgaatt ataaggagga agcaatggct
     7201 aagcagatgt ggaaacccgg tactatgctt tatcccgtgc cggccgtaat ggtatcctgc
     7261 agaagcagag aaggtgtgag caacatcata acggttgcat ggacgggtat agtctgttca
     7321 gaaccggcaa tgctttatgt ctctataagg cctgaaagat tctcccacgg cataattaag
     7381 gataccggca gcttcgtagt aaatctcccc accgaaagcc tggcatatgc ccttgacttc
     7441 tgcggggtaa aatcaggcag ggatattaat aaatttgagc atttaggcct cacccagcaa
     7501 aaatcaaatc tggttgatgc cccttccatc ggagaatgtc ctgtaagcct ggaatgccgg
     7561 gttaataata taatatccct gggctcccat gatatgttta tcgcagagat attatgcgtt
     7621 gatgcagagg aaaagctttt agacaagtcc ggaaagctgc acctgaataa ggcaggcctg
     7681 atatgctata accacggcga atacagggct ctcgcgaagt ctttgggcca tttcggtttt
     7741 tcagtaagaa aaaagaagta acggacatag ggcaggtgag acagggaggg acacggggac
     7801 gtttctccct gtctcactga aatttatttt ttattgcttc atttattatt tctgcctttt
     7861 caagcagtga gactgatgat atattgtaat tctccgccag cttatgttcc gatgcttcag
     7921 cattgcacaa acggcagtaa gtatgctcca atgcggctgc ccatatgttt acatcatcta
     7981 tttccggtac ctccgggaat gtcgacttta tgaattcata ccagatgtct tcaacaatct
     8041 tcttataagg cctcttatat ttatttcgca tcattgatat ggctttacgc ttcaccttta
     8101 tccatggctt ttcccattca gactcgggga aatccagaga atctatcgta acatttctct
     8161 tttccccgtc aaagttcaca gtatacggtt cttttgcacc tatgaaattc aggagaaata
     8221 ctgcttccat ctttatataa ttctctacat catccctcag catgaatctt ctgataaagc
     8281 tttccgcctc attcatcctg tcattcttta tcttattgaa tattagtttc ctcaaaaaat
     8341 atttatcaaa attaattcca aagtaaagca tgccaagcat atgctcattg tctgccaact
     8401 gttctctgca cttaggcccc ggcatttcaa taaacttgta tataaaatca agcctgtttt
     8461 tgatttcttc ctttggaagc tgatataaat aaggaaaata tgttctttca tcttcagcat
     8521 ccaactgatt tttggcagct tctatataat aatccgagag catattttct ttgtcaatct
     8581 ggcccagctt ctcccatagt ctgatgcttt ccctgtactt tctgctgttg aaagccgcaa
     8641 ctgctgcaag gtgtacataa ataggatttt tagaatcaat aattaaaagc ttcttgaagg
     8701 ttttgtatgc atcggcgtgc tttcccaagc ttccataggt atcacctatt ttatacaggt
     8761 aatcggcact gtcagcccgg atcctgctga ttatccccaa ctgcttcttg accaggctgg
     8821 ccgcgccctt cttattgtag aatatggcaa gattacagtt tgcatgcaca ttggcctctt
     8881 cataggtacg cacctccagg gcaagctcta ttgctttgtc gataaatccc aggtaataat
     8941 atgccaaaga gagattattc cttgccggta cggcgttggg aagcatctgt acaactattt
     9001 caaatttttc ggtggcctgc ttgtattcct tcttctccag gtgatcaatt gcctcttctt
     9061 caattttata tattttttca agttcctcat catccatgtt gttattggca tcctttatca
     9121 ttgtaagcat ctccagcaac tcttccgcct cctctgcgaa ctcgccgccg ggatcctttt
     9181 cgagataaat gctgaaatac tctgaggact tttttatctt ctgcagctgc agatagttgc
     9241 agcccaagcc aaaatagcac tccagataat tagggtcgat gtttttaact atattaagca
     9301 aaacctctgt ggaacggtcg atatgcccct gttcggccag cagtcccgca taattgaact
     9361 gcaggaagct gtcatgaggc ttgaggctta cagctttttc aatatatctc agtgcagttt
     9421 tcagcctgcc cttttgtata tacttgtatg acaggtcgaa ataaaaatct gcccctcttt
     9481 caaaggttac caccttgttg ttcatacttt catcaccaac tttaatgtaa aatatagatt
     9541 aatacttcat aaaacccgga agcactactt tgaaaagatt cctcttctca ttatttatga
     9601 acctggcacc accatgctgc ttgaaggctt catgtatttt tttcatgcct gcccctatat
     9661 taaggaatct ccccttattg tcaagtatca gcagcctttg atacagccac tgatttcttc
     9721 tgcagggatt gtactcctcc ataagcccct gtatgtcgtc atcgctgcaa tttgcgccgg
     9781 gattgcttat ttctatcttt ttattatcta tcgatatgac tatctctctt cccggagaaa
     9841 aataatctct atggacggca gcattggcca cagcttcaac aacagcagca actggatatt
     9901 tcccatttat tttctctgct ttaaatctca ggtagtcttc aatttcatcc agcattctaa
     9961 taatcggccc ggaaaaatat attaccttat cagccgcaga tttgtcaata agccttattc
    10021 cggcatgagg aaggtgcaat tggggattat aaccgaaaac cagcagccct cccaccgtag
    10081 ggtgataccc gttggagtcc tcatctcttc ctattatgcc tattccctca agaagtgtat
    10141 aatagttttc gctgctgctg gtaaaccctg ttttggaaat ataatcactt attatatcct
    10201 cgttcagttc cttcaattcc accctgttca gcacaattct ttcatactgc agtatccccg
    10261 attcctggag catgctggct atctcttcgc gccttgctat atccgtagta gaacctcttc
    10321 ttatgtaaaa tacacctgtc tgtcttatct gatgcggttt ctggctgctt ctgtatattg
    10381 tcaggacagc aatctgctta tcatcaactg ggattatctc aagcttcacg gataccggcg
    10441 ggtcacacct ctggcttatt atctgctgta tctgctcttc tgtataacgc tttccctcaa
    10501 ttcccaatat agcttttgtc tcgtcctgta tcccgtaaat tatatagccc ctgccgcctt
    10561 ttgaattagc tattgctgca acatccttgg ccagctcctt tttctcactt tcagttttta
    10621 atgaaagtac ggctttaaaa tccagcttgg taccttccgg cttttgtaaa agagtgtaca
    10681 gcttctgctt atccaaatga ttcactcccc tgttatcatt tcgcagcctt gtatccggtg
    10741 atattatata tagtttagca taacatgaaa aattatcaaa ataataagtc gcgaacaaat
    10801 ttaaattttc attcattcgc gactaatatt ttttgtatag gcatttggca ataatattcc
    10861 ggatatctta aaatgttttc cttgccatta gatcatctgt tgattgtgtc cgagggtctc
    10921 tatatccgaa atcatcacct tttaaatact tcagattata ttccaatgac tgatacaatg
    10981 tatacccgca gtcattaaga tctctgtttt ccctgtcctt gcactttagc cctgtcaata
    11041 acttaatgat catattcatg cttttacctc ccctgtcaaa cttccttact gtagtatttt
    11101 caatagaacc acacactctt gctattgtca tttataaccc ctcctttcaa aaataaaaaa
    11161 gccacaggtc tctgcccatg acttttaaag cgtcttcata taaatacaaa gtcacgggtt
    11221 ttacggcccg tgaccaaaat aactatcgct attattcaaa tcccaggtcg tgatatataa
    11281 tttggggcac actgatgcca tatacaaaac aggctgcttc ttttactgca gtactaatca
    11341 tcattattac gcttctgttc atattcacga cctccttttc ttgataacat atcatgctca
    11401 taccggcata attttttatt actaataaaa tataccacca aattgataat tttgcaatgc
    11461 ttccaaatat tacaactgtg taacagagtg tactttactt cttgatattg tttttatatg
    11521 cgtatattgc cgcttgtgtc ctgtcactga cgtttatttt tctgaatata tttgaaacat
    11581 gattttttac tgtcttttca ctgataaaaa gcctgtctgc gatatcctta ttgttcaaac
    11641 cgtctgcaat aagcacgatt acctcatatt ccctctgggt aagctcattt tcattcttaa
    11701 ccctggctcc tcttggcttg ttgaattcct ttatcaaatc agcggccagt ttcggctgta
    11761 tatatgactc ccctatatat gcatccctga tggccttgaa gaaacttgcc gaatcggaat
    11821 ccttgagtat ataacccgaa gcgccgattt gcattgtctc taaaaggtat tccctgtcct
    11881 catgtatcgt aagtatcacg actcttgtgt caataccgtc ctccttaagc ttcttaagag
    11941 cttttatacc gttcattata ggcatgttta tatccagaag aagcacatcc ggcttaagct
    12001 cctcgacttt ctctatggtt tcaactccat tagaagcctg tcccacaact tcaaaatctt
    12061 cctccagctc caggagctgt ttgagtcctt ccctgacgat ggaatgatca tctgcaatta
    12121 atatccgtat attactcaca ttatccctcc tctgctatat tcaaagatag gcttgcaaat
    12181 actttagtgc ctttattacg ggctgtcttt acgtcaaact tcccgttcaa cagttcgact
    12241 ctttccttca tattgagcaa gccataacct gcatcttctt cttttgcctt ccacagcttg
    12301 ttcttgtcaa agcccttccc gtcatcactg atgagaaggt taacctggtc attggtaaac
    12361 tcaactctga caaccgcatt atcagctccg gaatgccttt ttatattggt caatgcttcc
    12421 tgcacgattc tgaacgcagc aagctcaaca accggcttta atgtcttcgt atctcccaaa
    12481 accgaaaaat tcaccgatat gttgttttgt tcgatgtagt ttgaaatata tctttgaagg
    12541 gtgggaacca gtcccaaatc gtccagagac ataggccgca gatcgtatat gatctttctc
    12601 acgtcactca ggctggaacg aaggaaacct ctgagactgc caagttcctc ctttgctttc
    12661 tccttatcca tatctataag cttttcacat aattcggttt tgataaccag attggccatg
    12721 agctgcgcag ggccgtcatg tatctccctt gcaactctct ttctttcttc ttcctgtgct
    12781 ttgattatct ttatacccag atattgcttc tgctttatat cctccatctg tatcgaaagg
    12841 tcctgcagct ctccggtcaa atagcccatc gccactccaa ccttgttgac aagagattca
    12901 gctttgtttg cagtgtctct tgctatctta agccttcttt ccagctcgct tctccgttgt
    12961 ataagcagct gttcctccgc ttttttcgtc atcagctcta cctgaagatc ctttgcctta
    13021 tcgtaggctc tttttacatc ctcttccttg taatccttaa tattcttact cactatcata
    13081 agctgatatc tgcattcctt gtcaagcttt accagcatgt ccacctgatt gataaggtct
    13141 gatgcaatta tcctgatttt ttccagttct tcctctacca attgacattc ggtgcgggag
    13201 ctttcagcta tgctgaataa ttcacttctg ctcttttcaa taatatctac cgtatttttt
    13261 atgatttcat ttaacctgtc cacaataagc accctctgct ttcttccaaa taatcaatac
    13321 tgcaaaatat acctcatatc aatattatcg ttgaataatc aggaatcatt tataaaaaaa
    13381 tttatttttt cgtgtatttt agaaaagtgc ggtaaaaggc cgcactataa aaaagtgttt
    13441 aaatggcagc aagctgtgcc tgttcctgct catcaaacaa aaaattcaaa tcaagcatta
    13501 taatcagcct attccccttt ttccctatac cggatatgta tttcctgtca accccggcga
    13561 ttatatccgg agtttcctgt atgccggcct cgtctaatgt cagcacttcc gatgcatcat
    13621 cgacaataaa ggctatctgc ctgtccccta aattgataat aatagtcctt ctgttttcat
    13681 ttaaatcccg ctctggtccg ttcaaattct cttccgggat attgaatctt tttcttaggc
    13741 taattaccgg aataacatta tccctcagat tcattattcc ttcaacgaat tcaggagtat
    13801 tcggcacctt gaccggttct gaataatggc ctatttcctg tacttgcatt atgttcacgc
    13861 catattcttc cttgcccagc ttaaaaacaa caaattgcaa ttcagccata aaaatccctc
    13921 ccaaagctaa ttatatcact aaagccatta ttgcacaatt ctactgtttt ttacatttac
    13981 atccgcaatg gtcatgatgt tcatgtccgc agtcatgcct gtcctcggca aatttgattg
    14041 aatccagccc atgccttacc tgtcctttta tatattcaat atacttcctt ctgagttcct
    14101 gctgctcctg ccgttcttct tcggttaagc cagcctcctt ggactttttt gacagatgat
    14161 ttatcctgtc tatcatttct ctgcttatca tattatatct atcctatcct taaatttact
    14221 atatcaagta tctcactatg gagcttgcgc ccttcggcag aaagcctcct gcatttatca
    14281 taggcctctg acctgactgc ttcatcctca attccggaaa gattgatatt cacattcaat
    14341 atcgcccctt ccagccctga aagtgtcaat aaagcgccta ctcccacatc ggacacggcg
    14401 ttcctgttgc cgtacctggc aatattccct tgattttcaa gcagccggaa acagcattct
    14461 gcggtcttta tgggaacctg aagtgcataa aggcttgctt cctgcatttt ctttccacgc
    14521 agctgatttt gttcttcggt atcctttgga agctttaccg cctccatgaa tttgttgaag
    14581 gcttttgtat cctcgtcaac caaaccggaa agctcctttt gaagcgcctt tacattttcg
    14641 gcttcctcca tgaacttcgc ttttattttt tcattcaggt tttcataatt ttttttgccc
    14701 actgaaagat tcactaccat catagtgagg gcagccccca gcacccctga cagcgcagcc
    14761 gtgcttcctc ctcccggggc aggttcgctg cttgccagca catcactgta atcccttaaa
    14821 gtcatatcca ttaaaagact catatattcc tccccatatc tacttcaaaa ttcttccgtt
    14881 ctctacgact gtttttccgt ccttgataac ggtatccaca tggtttatgc cgaaatggta
    14941 tactatatat tcaagatttg gagcatcaaa tactacaata tcagcttttt tgcccacttc
    15001 gaggcttccg gtttctcctg ctctgtctaa ggcataagct gcattcagag tcattccttt
    15061 tattatctcc tcgggagtca ttttcattcc aaagcatgca aaggtcatta tggactgaag
    15121 attctcggta gggcatgtcc ccggattgta atcggttgcc agtgccaccc tgacacccct
    15181 gtccatcatc tttcgcgcct gggcatactt gccaagcatt aaataaaagg aagttcccgg
    15241 aagcagcacc gccgtaactc ccgcttccgc cattgcggct attccggcct ccgttgcagc
    15301 cagcagatgc tctgcagaaa cggcgcccaa ttcccctgca agctccgccc cctgcagagg
    15361 gactatctca tctgcatgga ttttaagctt gaagccttgt tctttgccct tctgcattat
    15421 ggccctggac tccccgggag aaaaaacccc ctcctcacag aatacatcca taaattcggc
    15481 aagattgttt tccctgacat aaggcatgac cttatcggcc atgaaatcta tatagccttg
    15541 agtgtttccc ttgaattcct ccggtacggc atgggctccc atatatgttg acactatgtc
    15601 tacgggatgg gagtcattaa gctcggcagc cacctcaagg cacttgatct cggtatcaaa
    15661 atcaaggccg taaccgctct tggcttctac ggtagtagta ccgtgaagca gcatctggtc
    15721 caggctcttt ccggattttg ccttaagctc ttcctttgaa gctttccgtg tgtttctaac
    15781 cgtgctaagt atccctccgc ccattttcag gatttccaga tacggcactc ctttaagctt
    15841 cagaggaagc tccttttccc ttgagcctcc gtgaacaaga tgggtatgcg cgtctataag
    15901 ccccggagtc actgtcttac cggctgcatc aataatcctt gttcctgagc ccttgatatc
    15961 cccgggggca tttccctctc ccagagatat tatttttccg tcttttacag ccacatatcc
    16021 gttctcaaat actcccgcac cttctccttc ggtacagcag actattgttg aagcatttat
    16081 tatcaagtca gctttcattc atatcactcc aattctccaa ctgcgctttc cactgctctt
    16141 atcaaagctt cggacttaac catctcatcc agcttattca tgtcggtata cattatcctg
    16201 tcctcttcaa caggcgcaat ctcttttctt ataagcttgt aagcttccga ggtacctttt
    16261 ccgagcctta catcccccct gaagcttatg gcctgggaag ctgcaaatgc ttcaattccc
    16321 agcaccttct gaacgttgtc caatataacc ctggcctttc ttgcggcggt agttcccata
    16381 ctcacatgat cttcctggtt tgcggaagaa ggtattgaat ccacactggc aggatgggcc
    16441 agaatcttat tttccgacac aatggaagca gccgcatact gggttatcat aaatcctgaa
    16501 ttcagccctc cctttcttgt cagaaaagca gggagatcat taagctgagg atttaccagc
    16561 ctttcaagcc gtctctccga aacatttgcc agttcggata ctgctatacc aaggaaatcc
    16621 atggcaagag ctataggctg tccatggaag ttcccgccgg atattacctg gtcatcatcg
    16681 gcaaatacaa gggggttgtc cgttgccgca ttaagctcta cggatacctt ttcctttaca
    16741 tattgcacag cgtctctgct ggcaccgtgt atctggggta tgcaccttaa tgtatacgca
    16801 tcctgcaccc ttatctgtcc ctgggttgtt gtaagctcgc ttccggatac tatcctcagc
    16861 atatttcttg cagctgccat ctgacccttc tggcctctta cttcatgtac cttcctgtca
    16921 aaagctgttg ttattccctt aagtgcttca agagtcatag ccgctattat atcagcgttt
    16981 ttcaaaaggt cttcggcatc atatacggca agggccccta tgcctgtcat taccggagtg
    17041 ccgtttataa gtgcaaggcc ttccttggaa gtaagctcaa ccgttttaat tcctgccttt
    17101 tccatagctt ccttacctgt catccctact cccttatagt atgcctctcc ctctcccagc
    17161 attacaagaa ccatattggc aagaggtgca agatcaccgc tggctcccag ggaacctttt
    17221 tcggggatca ccggatgcac tcccttgtta agcatctcaa ccagtgtatt taccgtctcc
    17281 agccttatac cggaaaaacc ctttgccagt gcgttagccc ttaagagcat tattgccctt
    17341 acagtctctt cggtaagcgg gttccccagg gaacaggcat ggcttacgat aagattcctc
    17401 tgaagtgctc tggtatcttc ctttgatatg aatacatcgc tgaatttccc aaagcctgtg
    17461 gtaattccgt ataccaccgc ttccttatcc accaggctgt ccacataatc tcttgatttc
    17521 cggattcgtt ccactgcctg ttccgacaac ccgaccctcc ggctgtttct cgcaacatct
    17581 acaacatcct ttatagtcaa atgctcaccg tcaattgaca aatgccccat ctcagaatac
    17641 actcctttat cattattatc tgtcaaatac attttatcag aattacatcg tcaaagtaaa
    17701 ataaaagata attattccca tttgttgaaa tgaatcctat tttcatcata acgatcagaa
    17761 ggcttctttt tttccgcagg gtgccctact gcaattacat tcagcggtat catattctcg
    17821 ggaatcttga agatttttct tatgccatta accctttcct ccaccgggta tactcccagc
    17881 cataccgtcc caaggccttt gctctgcgct gcaatcagta tgttctcggt tgccgcggaa
    17941 caatcctgcg cccaatagcc cttatatctt tcgttatcca gtttaccgca tacgattatg
    18001 gccaagggtg cttgcagcag cattgccgaa tagggatgaa ccttggtaat ctccgtaagc
    18061 agctgcctgt cattgacaat gataaattcc catggctgcc cgttccctgc agagggcgcc
    18121 gccattgccg ccttcaggag gtctttcaga tcggcatccg ataccggctc gccggtatac
    18181 tttcttatgc ttcttctttc caatattgct ttcatataat cactcccttt taacttttct
    18241 cagcgtcttt ttcgattttc ccttcttgga ttccttcttt tctccggatt ccggggaggc
    18301 tttataaggc acttctcccc aaaacctatc agatcttctc tgccggctga tttcagcgcc
    18361 ttatatacca gcccgtagtt ttcgggattc ctgtattgta tcaatgccct ctgcatcgcc
    18421 ttttcctcgg gatccctggg tacatataca ggcttcatcg tcctgggatc aagcccggta
    18481 taatacatgc aggcggacag tgtccccgga gtcggataga attcctgtat ctgctccggg
    18541 ttataaccca tatcccttac atactcggca agctctacag cagcggaaag gtccgaaccc
    18601 ggatgtcccg acataaggta aggcaccagg tattgctcct tcttcagcct tttgttcata
    18661 tcaaagtatt tttttacgaa cctgccgtat acttctttgg acggcttgcc catatacctt
    18721 aaaaccttgt ccgaaacgtg ctcgggtgcc accttcagct gcccgcttat atggttttca
    18781 caaagctccc tcaggaattc atccttcttg tcggcaataa tatagtcata tctcagtccg
    18841 gagcggacga ataccttttt taccccggga agggatctga gctttttaag cagtgcgaca
    18901 taatccttgt gatccacctt gaggtttttg cagggagtcg gataaaggca ctgtctgttc
    18961 ttgcagactc cggcattaag ctgcttatcg caggctgcgt gcctgaaatt ggccgtgggc
    19021 cctcccacat catggatgta gcccttgaat tcagggtctt taaggagcag ctccgcctct
    19081 tcgattattg attcatggct tctgctttgc acaaccctgc cctgatggaa gttcaatgcg
    19141 cagaaggaac agcctccgaa gcatccccgc tgggaagtaa ggctgaattt aacttcctta
    19201 agggcaggta tacctccctg attcttataa accggatgat actgcctggt aaaaggcaga
    19261 ctgtatacat cgtcaagctc tgtttccgtc aggggtgcgg cgggaggatt ctgaaccagg
    19321 aacctttccc catgaggctg tataagtacg cggcccgtat gatagtcctg ttccctgtat
    19381 tgaagcataa aggcccgggc atatttcctt ttgctgtccc taacctcatc gaagcttggt
    19441 atcacagtcg catccggaat attcccaagc tccggggcta tatatgcagt accgtttata
    19501 taggttatat attttatttc catacctgat tcaagtgctt ccgccacttg tatcacgggc
    19561 ttctcaccca ttccgtatat cagaagatcc gccctgctgt cgaacaatat cgaccttctt
    19621 acctgatcat cccagaagtc ataatgggca aatcttctaa ggctggcttc aatacccccg
    19681 attattacgg gcacatcctt aaaggcctcc ctaatcctgt tgcaatatac ggtaactgcc
    19741 ctgtccggcc ttttaccccg tacaccggcc ggagaataca agtcttctct tctgggtttt
    19801 ttaccggcag tatagtgatt gaccattgag tccatatttc ccgaggacac aagaaatgca
    19861 agcctcggtc ttcccagagc cttgaaggcg tctgcattcc gccaatcagg ctgcgggatt
    19921 atgcccacct tgtagcctct gctttccaga agcctggtta ttacggcact gccgaagctg
    19981 ggatggtcca cataagcatc cccgcttacg aatatgaaat ccaactgctc tatttttttc
    20041 ctctgcatat cttccttgga aataggaaga aaatcatccc tgctaatcaa gaaatcacgc
    20101 ccttgtacat ttggatacgg ataacactga acacacggat gtttcatgga atcgctgcgc
    20161 gattgcacgg ttaactttca tatattttta tagctgcatt cgtaaatatc gatacttaca
    20221 atgttatcga gtattattta tgtaacagaa tatttatccc gccgtgtctt ctgcggattt
    20281 ccgtgaattc cgtgttacct gcccctcata atctatttta actgaaatgt cgaaatcggc
    20341 gtataaatcc tctttcctcc cgcttcctca ctttttatca aggatatctc cctgatttca
    20401 acggttctga agttttgaaa attcaacttt tcctccagct tttcaaaagg tattttgagt
    20461 accagatcct gggctattgt tatatgcggg gtgtaggctt ttatctcctt ttttatgccc
    20521 agcttgtaca tcccctcatc aatgtccgaa tacaatgagg agagcttatc cagttctcct
    20581 gaaaaaccca gccaaagagc atggatgctt ccttttccgt caaagtaccc tattttatct
    20641 atgttcagac ggattctcct gtgctttaag gctatttcct taagcgttct ccctatgtcc
    20701 ggtgctttgg cctgctcaat ttcgccaagg aacttaagtg tcagatgaaa gttgcctaca
    20761 tacttgaatc ttcctctttc cgagttttcc cgcactatat gctgtactcc cgcaatgctg
    20821 tcctttatat ccctgctgaa gtccgcccct ataaatgtcc tcataatcaa gccctcctat
    20881 agtcaaaata tcattccatc attcccaaaa gctttttaag cataattccg tttattacag
    20941 cctgtgccct gaaatgattg ttgaagctga caaaacaatt ctgagattgt ttgtcaagct
    21001 ccaatatctt atccttccac tgctcaagct ccctttcctt atacaaataa tcataccttt
    21061 catatgcttc tttatgatcc caccatttcg catcgttcct tccgtgaaac cgtatatatg
    21121 agatttcgga agtcctcatt gccctgggat ccaaaagccc cgacacagcc ggctcatcca
    21181 cgcagatata tcccaactgg tttgcccgca gaaactccat ggtttcgtcg ttaacccacc
    21241 tgttattcct gaattccacc attaaatcaa aatcagcaaa tctttctctc agtccttgta
    21301 tatagtccat attctccctg ttaaaatgga aagaataagg gaactgcgcc acaaggcatc
    21361 ccagcttccc ggcctgcttc agcacatcca ctgccccggc aaattcctcg taatccttgt
    21421 ctcccgcatc tcttccgtgg gtcatggact gatggagctt aacggtgaaa ataaagttgt
    21481 ctcccgtctt cttctgcata ttatagaaca taaaggaatt gggtatcttg taataagtgg
    21541 aattaatctc cgtaaaatca aaagagcgcg aataaatacc caataaatcc ccggccttgg
    21601 ccccggccgg ataaaatgtc cctatccaat ccttgtagct gtatcccgaa gttcccacat
    21661 gtatcataat acccctgccc ccatgaattg tttcagcaat gaaaaaagta tgcagccaac
    21721 catgtaaatc gtatattggt gcataccttt attgtactat tgctgtaata aataggctac
    21781 atcgttttta tctttttttc aaatccgctg ctctcaatat ctcgctgtag atttcgtcct
    21841 cggttttgcc ctcggcatta attacctttg tagttgtcat cacatcttcc atgcccatga
    21901 aattggaatc cgcacctgtt acgacaatgg catcgaattt gttcaggttc ccgggggatt
    21961 ccttggtatc gtcctccagc acttccacag cgcagtccct gctttccaga tatcttccga
    22021 cgttgctcaa gggcttttct attcctacct tgttcatatc aatacctcca taaatttctt
    22081 cataaatata ttctcctcta aatatttact ttttattcga tatcttctag tgccctgaaa
    22141 tacatcattt aatgcaatag ttccaaaact ttgataattt tgggactgta cattcgggca
    22201 aatttagtct aaaatggatt ttgagcatat taaaaaatag ataattttga ctccattgct
    22261 gatttcaacc tgtatattta atgatgcagc ttgaaatttt tgtttctgta tgaattttaa
    22321 agcattataa tgtcaaagga tgattcattt gaaaaaagca ctggtgaaaa acaaaataaa
    22381 ctatactacc ttcatgttta ttcccgagag ccaatccaat gttaaatcct tcaggacgcc
    22441 ggcatggttc cccggatttg tttccatatc cattgcactg cttattataa cgtccggaat
    22501 aagtttttat gcattatccg cactcaagct tcaatatgat gccagtagaa aagaaatatc
    22561 aaagcttaca accatcaacg cttcccagaa gattgaaatt gggaatctgc agagaagttc
    22621 cgaagaagta caaaagcaat tggccgaaaa ttccagaatg ctggatgagg tcaaaaaagc
    22681 ggtgggtatt aatccctccc ctgaggaagg catcgagatc aaactcgttt ctccggaaat
    22741 cagttctccc gtaagcttgt taacagaaaa atcagaccaa gatatgtcct tgaaactagc
    22801 aagcattgag gctgactttg catctttaac cgataaaatt gcaattcaaa aaaagacaat
    22861 agctgattcc atagatccga taaaaaaaca gcttgcatat ttaatggcca agccctccgt
    22921 caaacctgtg gccgcctcaa taactgcaag ctttggctat aggaaaaacc cctttaccag
    22981 caggggcagc gaattccaca agggaattga ctttgcggcc tcctacggac agactgtttc
    23041 agcaaccggt gatggtatag tatccttcgc aggctggaat gccggctatg gaagaatggt
    23101 gataatatcc cacggatatg gcctcactac cctgtatgcc cacaattcaa agctgttggt
    23161 caagcaggga gacaaggtca agaagggaca agccattgca aaggtaggca atacaggcag
    23221 aagcaccgga acccatcttc actatgaagt aaagctgaat ggaaaaaacg tcaatcccgc
    23281 aaactatttt aactgatcag aagcattgga ttatggagat tggaggtagc aaaatgtttg
    23341 gctcaggcaa aaaatcaata aacagtgaaa gagtagatac aattataggc cgcggaacaa
    23401 tatttgaagg tactgtaaac gcagacggta ctgtaaggct ggacggcaaa gtgcttggag
    23461 gccttaatat atccggcaac ctgattgtcg gagaagaagg tgctgtcaaa ggcaacgtaa
    23521 agaccgaaaa tgcatttgtt gccggagtca tagaaggcaa cgtaattgca acctctcaac
    23581 ttcatattac ccatactgca aagcttgtag gcgatatcac agtaaaaaac atcattatcg
    23641 atgaaggtgc catattcata ggcaactgca agataataac cgataacaca taggaacagc
    23701 ggaaggacag ggcgcccccc ctgtccttca tttattcact gcataagaaa tttatacagc
    23761 attgcttact accgcaacaa tcctgtcaag aggtatatcc gttacagagc ctaccgaacg
    23821 cacattgcta aagcctccgc tgcagccgat tgtaggacag catggactat gacatgcatt
    23881 gccaagggca catcccggag ccggccctat gtgggttaca agcctgacga agcattggtt
    23941 tacagcaagt attacacctg taaaaccgca gcctgactgg cctccgcttg tagtgaatat
    24001 tgttacagtc tctcctataa ggtctgagag taagtcaaag aaattgccgt taaaatttcc
    24061 gccataacca attccatttg ccattgttac accccctttc gcataattgg gtttctgact
    24121 ttcccaattc cataatatgt acatgcattt atttggtgac agcctgtatg aacaaaaagt
    24181 tcagagtaat aggaaggaca ggtgacttcc cctgcccttc gatacagctt cctaaatggc
    24241 attacgtaca actgcggcaa tcctgtcaag aggaatatcc gttaccgatc ctaccgttct
    24301 cacattgcca tagctgccgt aacctccggc gggtactccg cagcagctgc ccaatgcaca
    24361 gttcggagcc gctcctatat gggtaataag cctgacaaaa catggattta cagccagtac
    24421 tactcccgta aagccgcatc ctgacaggcc tccgcttgta gtaaatattg taacagtctc
    24481 tccaatgcta tcctgaagaa gatcaaagtt tccgcctcca aaagcattgc ctgctactcc
    24541 gtcaaccata tttacacctc ctttcaagaa caaatctggt tatatagctg tgccattgtt
    24601 aaaaccgaaa gttcttccaa aaccgccaaa agcgattatt gcaagtatta ttatgaagaa
    24661 gaagtttttc tccctgccca aggttgtaca tgatgctaag agcacaaaca acagaagcag
    24721 caagaggaat ccgttaccct tgcacttatc taagatcgga cttactgacg caggagcacc
    24781 gcatccgcat ccggttgagc ctaatccgtt tgccatgatg tcaccccctt tcatttctat
    24841 tttatgtaca tgcgtatatt tggtgacaag tttcccgctt aaagcatgaa agcgggcatt
    24901 aaagccccct tccatgcttg aggtgagtta tggcatctgg gtcaggactg tatggttgac
    24961 accctttccc aattccataa tatgtacatg cattaatttg gtgacaacaa aaaaatccgg
    25021 gacacggacg ggacacgggg acgtttcgcg tgtcccgctg aaagtgggac aggggaaacg
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtccccctgt cgttacagtt
    25201 taataatcat taccaccgaa tccgaaggtt cttccaaaac cgccaaaagc gattatagca
    25261 agtactatga taaagataaa gtttttctct ctgcccaaag ttgtgcatga tgctaagagc
    25321 acaaacaaca gaagcagtaa gaggaatcca ttgtttttgc ataagtccat aatcggactt
    25381 actgctgtgg gagcgccgca tccacatccg gttgagccta atccgtttgc catattttca
    25441 cctcccctca ttataatttt tgtattttat atggcaaacc cgccaaggcg aaagccaggc
    25501 aagatacccg ggaccaggaa gattatcaat ataataatcc aaatccagct aaatcctccg
    25561 aacaagcctc cggcaccggt acaccccttg aatccaagcc gcggaagtac aaatatcaaa
    25621 agcaatatta tccaaatcca tgcattgctg aatccaaaaa aacctccgac aggcgccggc
    25681 gatgccccac accctgcggc tgtgaccgga ttggccactc caaatccgct tgccacagtt
    25741 caaaccccct cccggataat tataggttgt tcacctttcc acttatatac tatgtacaca
    25801 tgagtgtttg gttacatttt tttaccgttg gctttctaaa atatatgtat taattcgaac
    25861 atatgttcgt ataatattct aagaaatatc ctttattctt atgaattaac cagataaatc
    25921 ggcagaattt gcttatacaa tgctacagcg aagtttctga acaggcaaaa acatgctaat
    25981 attaaagcac ataagaagaa gaatgcgacg gggacacggg gacgtgggga cacggggacg
    26041 tttcccctgt cccactgaaa gcgggacagg ggaaacgtcc ccatgtcccc gaaacgtccc
    26101 catgtccccg tcgcattctt cttctattct gcacaacaag aaaattattc catataggag
    26161 gtgaactaaa tgaagatgct cagaaagcct gttgaggcaa tagtgctttt tgatctggaa
    26221 ggcaatcctg tacctgtaag gttcaggtat cttgatgaca gatcggagtt gatcacggta
    26281 aaagtagacc gggtaataaa aagagagctt gacaagtttg ccggcaacag gatgctcaag
    26341 tttacctgcc agacacgcat cggcaatcag atcaagccct tcgaactcag atttgaaata
    26401 gatacttcaa aatggtattt atataagatt taactgtctg tcacagtatg ctgctcatta
    26461 cgggatagtc ttctccccct gaccctccgg acatgggggc aaactccccg tcggcaaata
    26521 tggccctcat tacggaatca ttcccatacc tgcctcttat aacatccatg gcactgtcca
    26581 gcgcctgctt cttgtatttg cactgctggt caaatattga ggcctgcatg aactcgtcgc
    26641 tgcacaaatc cgtcactctt acccccagat gccttatttt ttcacctccc cagcattcgt
    26701 cgaacagttc tcttacagtc tcgaatatat ccatcgttac attggtgtga gccataagct
    26761 ttctttgatg ggaataaaaa ttaagctccg ctgtccttat ctccacagat accacccggc
    26821 agcaggctct ggcagccctg agccttaatg aaacagattc cgtcagggac aataatacct
    26881 tgtgggcaat ctgcctttca gttacatcaa actttatagt ggtgctgttg cctattcctt
    26941 tcatctgcag gtaatatccc gggtataccg gcgattcatc aataccgttc gcataacacc
    27001 atatcagatt accgaaggat ttaagcctgt ctctcaagaa tcttctgtct gccctggcca
    27061 agtctcctat tgtggttata ttcatttcca caagcttctt tctggtctgc ctgcctacca
    27121 taaataaatc gcctacatgc agcggccaca ttttgctctc tatctcgtca ggaaacaaag
    27181 tatggaccat atcgggcttt tttagatctc ctgcaacctt tgcagttaac ttatttgccc
    27241 ctatccctat attgacggtg aagcccaatt cctttttcac cctctccttt atcatatgtg
    27301 cggcggcaac tgaatctccg aagtggggtt ccattcccgt ataatccaaa aacaattcgt
    27361 ctatggaaaa tctctgcaaa ccgggagaat actccttcag agtttcatac agtatattgc
    27421 tgcatttcat gtaaaggtta tagtcagggg gcaccactac aagcccgggg cacttctgcc
    27481 tggcctgcca cagcacctcc ccggttgtta ttccaaactt tttggcaggt atgcttttcg
    27541 ccagcactat acctctccgg tcagcctggg agcctcccac taccgaaggt atttccctaa
    27601 ggtcaagggt atcccccttc tgcaaattgt aaacagcctg ccaggataaa aaagccgaat
    27661 tcacatcaat atgcattatg gttctcatac cgactccgcc ttccctgcaa tcaaaaatcc
    27721 ccatatatta tgcgaacgta tgttcgattt aattatatca tcttttggca tggaagggaa
    27781 gtatttctga gtccggtcct atttaaaagt ttaatgggaa caagttcaga aggaacaggg
    27841 gatgtattcc tgctccagca tagacattat caccatagag cggtagctgt ccccatgctt
    27901 cttgcaatcc ctgagtattc cttcccgtac aaaaccttca cttctgtaaa ggcctatcgc
    27961 tttttcgttg tcggtaaaca catccagcca cagcctatgg catttcaaca cctcaaagca
    28021 gtattttttt ataaacttca cgcttgtccg tccatagccc tttcccttgt cggatatcac
    28081 catgcgtctg aactctacgg cactgtcctc tcccccgatc cctgaaagca atatgtaacc
    28141 caccagcttc cgttcggcag agctaaatac ggatatatgc atttcatcct cactgttcat
    28201 ggctttcatg tgcctttcct tcggccatac aaacacaaaa tcgctgttag ccttctcaat
    28261 atcaagtatc aggtcgagat attccgaacc ggtaaggcaa aacataagcc cgcccatttc
    28321 cataatcata tttttcataa cccgcccccc tcaggtttat tttataatat ggtaatataa
    28381 tatacaatat ttagaaggga ttgctgcaca aaatatgaat gatgcggcat ggggaagttt
    28441 ctcccgggga cacggggacg tttctccgag gacatgggga cgtttctcct gtcccgctga
    28501 aagcgggaca ggagaaacgt ccccatgtcc tcgcgtcccc atgtcctcac atatatttat
    28561 atgttaatag ccttgtcaaa tctctccatt accatttttt cacccataac ctgcatgatc
    28621 tcataaaggt cgggagtatt cctcctgttg gtaacagcta ccctgataat tcccgtaaca
    28681 tcacccacat ggcctttata ggcctccgga tttttcttga aggtcttcac atccttggag
    28741 taccccattg cctcgcagaa tccctttact ctgtcaaacc acatatcctt gctgtcggaa
    28801 tggttataaa cctttgcata ttcccttata agcctgttaa cctcatcggc aggtattgtg
    28861 tcaggaaaat tatatccctc tgccgcatcc cgggtaaaca gctcatcaaa gaaatagaat
    28921 atattcggtt tcacatccag ccattttgcg atgtcttttc tgggcttttc gccgcctctg
    28981 tctatactga aaatcctttt tgcatacttc tcatctcttg acagaagggc ccaaagctca
    29041 gaatcatatt cctcagccca ttccagggac atattataga cctcttctgc agtcatcccg
    29101 gcaacaatat ccttgctcat atccgtaagc ttgactatat caaacatcgc accgcttaca
    29161 ctcattcttt ccagctcaat tttgaagctg gtattggggg actcggggtt ttcccttctc
    29221 caatcctcaa aattagaatt tatcaggttc gcaaggtatt caattacgga aatgcctggg
    29281 aagccctgct catgataaaa gcttacatcc gcctccgggt cctttctctt actgagcttt
    29341 cttttagagg aaccttccat cttcattatc ggggagatat gcgcaaaata cggcttttcc
    29401 cattcaagca tttcaaaaag ctgcacatgc agaggagccg aaggcagcca ttcatcgccc
    29461 cttgttacat gagtggtcct catcaggtgg tcgtcaacca catgggcgaa atgataagtg
    29521 ggaagcccgt cggacttcag aagtacaata tcctggaaat tttccggcat ttccacatcg
    29581 ccctttatga gatccttgaa aacaaacctg ctgtcaggat tgtcgggtgc ttttacacgc
    29641 agtacgaagg gcttgccccg ggaaagctct tcctttacct gctccagagt ggaattccta
    29701 tgcacggcgt attcaccgta ataaccgggg gtttccttct tctcttcctg ttgggctctt
    29761 atcttatcca agtcttcctc agagcagaag cagggatatg cgagcccttt ttccaccaaa
    29821 cgttttgcaa aagtcttgta tattttcatt ctttcacttt gcttatatgg gccgtagctg
    29881 cccttctcat cgtctacgtc tgttactcct tcatcaaagc tgatgccgaa cctggagagg
    29941 gctcttataa tattggaaac tccgccttcc acctcacgct ttttatccgt atcctcaact
    30001 ctcaggaaaa atactccgtt gctctgatgg gcaagcctct ccgatacaag agatgcatat
    30061 aagcttccca tgtgcacgta gcctgtaggg ctgggggcaa acctggttac ctttgctcct
    30121 tcaggcagct gcctttttgg atataacatc tcgtagtatt ccggtgtctt ttggatatca
    30181 ggaaatagca aatccgccaa ttccctgttt tgtgtcaaat tatgcattat aacaaaccgc
    30241 ctttcgttag tagtgtttcc ctaaggctgc gggcattctt gccctgtcag ctttgaggcc
    30301 catttctgag ctttataaag tatcctttcc tcatcaagag tgacaaggtg tccgctttcc
    30361 attaccaaat ttccgtcaat tataaccgtc tccacgtcag cggagttccc ggaataaacg
    30421 attgcggatt ttatattatg cttcggataa tagtgggctc tctcggtatt aagaactatc
    30481 aggtctgcag cctttcccgc ctcgatgctg ccaaggtttt caaatcccaa ggcctttgca
    30541 cccttcaaag ttgccattct aataacggcc tccgccggca gagccgtagg atcattagta
    30601 tatattttct gcaaatacgc tgccagcctc atttcttcca acatgtccag gttattgttg
    30661 ctggaagctc cgtcagttcc caggcataca tttatgcctt tctccagcat ctttgcgacc
    30721 ggcgctattc cgcttgccag cttcatattg cttcccggat tatgtaccac atttaccctg
    30781 cgggaagcta aaattccaat gtcgtcgtca tcaactgcca cgcagtgtgc cgctactgtc
    30841 ctgtcattca gaagccccag atcctcaagg tacttcaccg gtgaccgtcc gaatttttca
    30901 atgcaggtac tcacctcttc ctttgtctcc gaaagatgta tctgcacagg taccccttgt
    30961 tccagggact ttgccgccac cttcctcaaa tatgcctccg aacaggtata caccgaatgg
    31021 ggggccagca tcacctttat ccggccatta tagctgttat gaaacttccg gaacagttca
    31081 tcactttcct taagcctgta atccggctct tcttcccctg attccccctg aagccccctg
    31141 gagagaacag ccctcatccc ggattcggca gccgcacggg cggtgctttc cataaagaag
    31201 tacatatcgg caaaggttgt tgtcccgctt tttatcatct caaccatggc aagaaggctt
    31261 ccccaataca ccgattcatc attctgcttg tcttccaggg gaaatatttt tgtaaaaagc
    31321 caatccataa gtttcatatc atcggcataa ttcctgaaca ttgtcattgc cagatggtta
    31381 tgggcattta caaaaccggg cataaccgtc ctgcccttgc agtcaataac ctttgtatac
    31441 tctgcttcaa tacacccatt gctttcccct atgaagtcaa tccgattatc ccgtattccg
    31501 atactgcctt ccggtattac gtcatcatta tcattaaggg ttataatctg cgcgtttttt
    31561 aacaatatat ccatgtacta ctctccccag cctctaagat attcttcctg ctcctttgta
    31621 agcttatcta tccttattcc cagagcctta agcttcatat aggcaacttt tctgtctatt
    31681 tcctccggta ctttatatac cctgttctca agcttttcct tgttaaggct tatatatctt
    31741 gcggtaagag cctgcagtgc aaagctcata tccattatct cggcaggatg tccgtctccg
    31801 gcggcaaggt tcacaagcct tccctccgcc agcaggttta ttatccttcc atcctccatg
    31861 acatatccca ttatattgtt tctcattggt tttttcccgc agcttagggt ttccaaatcg
    31921 ggcttccaga tctcaacgtc gaaatggccg gcgttggcca gtacaactcc gttcttcatg
    31981 gccttgtagt gcttttctgt gatgaccttt gcgcagcctg tgacagttat aaagaaatct
    32041 cctatggatg ccgcttcatc tacggacatc acctgaaaac cgtccatata cgcctctata
    32101 gcctttatag ggtcaatttc acataccgtt acattggcgc caaggccctt ggctttcata
    32161 gccacaccct tgccgcacca tccatagcct attaccaccg cgttctttcc ggcaactata
    32221 agattggtgg tcctcattat tccgtcccat accgactggc ctgtaccgta cctgttgtca
    32281 aacaagtatt tgcagtaagc atcattgaca gaaatcatgg gaaatttgag aaggccctct
    32341 ctttccctgg ccttaagcct caatactccc gtagttgttt cctcgctgcc tcccaatata
    32401 tcaggcagca gctcctgcct ttcgccatgc agaagatgca ccaggtcgcc tccgtcatct
    32461 atgactatat tggggcctat atccaaagcc gcgttcagat gatggttata ctcctcagga
    32521 gtggcattat accaggcata tacattaagc ccgtcctccg ccagcgctgc cgcaacatcg
    32581 tcctgggtgg agagggggtt gcttcctgtt acggaaactt ctgcgccgcc cgctttcaat
    32641 accttggaaa gatatgcagt ctttgcttca aggtgaattg acatagctat tttcttgcct
    32701 gcaaaaggct gctcttctat aaattccttc tctatagcag acagtacagg catatggcct
    32761 tttacccatt ctattttcct atggcctgac tcagccgagc taatatctct gattatactc
    32821 tctctcattc ttaaacctcc ctatctcgca ccccgtacct cgtaccccgt accccgtgcc
    32881 cgtatctata ctctgcgttc ctgatcgcaa tcgtcgaact cctcggcaat tctagcaaac
    32941 ttttctatct ttttctgtac caaatggaat actgtatttt caggatagtt tccgctctca
    33001 tcccttgttc ctgcaggtac atcggtaaga atttctattc cctcctctat tttcttcaca
    33061 ggatatatgt gaaacatgcc ttccctgacg gcttctatga cctcatcctt caacataaga
    33121 ttatcaacat tctgccaggg tatcattact ccttcattcc ctttaaggcc tctggtttta
    33181 caggtctgga aaaagccttc tatcttttca ttgacaccgc ctatgggctg tatctcacct
    33241 ttctggttaa ccgatccggt aacagctata tattgcttta caggcagctc cgacaggctc
    33301 gacagcaggg catacaattc ggtgctggat gcgctgtccc cctcaacgcc ttcataaagc
    33361 tgctcaaagc atatgcttgc cgaaattgta aggggaaaat cctgcgcaaa cttgtctccc
    33421 aggtatccgg taagtatcag caccccttta tcatggatgc ttccgctcat ttcaacttcc
    33481 cgttcaatat ttactatgcc tttctctccg gcataggcgg ctgccgttat tctgctgggc
    33541 ttcccaaagg tgtaatcgcc gaagttcatt accgagagtc cgttgacctg ccctacaacc
    33601 ttctcttccg tagaaataag cactaccccc gtatcaatca gctcctgcat cttttcttct
    33661 atcctgcttg aacggtacca cttctgctca attgcccttt ttacactgct gcccgttact
    33721 ttctgttctc ctgctattct tgcccaggca tcagcctcat ataatacttc aattatatcg
    33781 ttgaatcttg tggaaagctt gtatttatgc tccgccaacc tcctgctgta ttcaattacg
    33841 gctgccacgg cgcttctgtc aaagtgcata agcccgtttt cctcacagtg agatcttata
    33901 aagctggcat atttcaaaca gtttcccttg tcactgctca tgtctgtgtc aaaatccgcc
    33961 ttgattttaa aaagctttct gaagtcctca tcatgggtgt aaagtatttg atatacatag
    34021 gagcttccta taagaataat ctttacgttc aatggtatgg gctccggttt tatacctccg
    34081 gatacaatac ctgacatgga aaatatattt tcaatagtta tgtttcctgt ttttatggat
    34141 ctttttattc cttcccagct ttcaggattt ctcatcaggt cggacatgtt gattattata
    34201 tagcccccat tggcctgatg aagcgaccct gcctttatct tggtaaaatc tgttgtgact
    34261 acccccatat tgctttcata ttcaatttta cccaatagat tatcatattt ggggtttgat
    34321 tccaaaatga cgggagcacc ctttgtatct ctattatcta ctgccaggtt aacctgatat
    34381 tttatggtaa aatccttctc ccttattctt tccaccaggt caagagtttc agccttaccg
    34441 acctccaggt tcctgaaatc ctctacatta tgtattatat ccctctgaac agcctgaagg
    34501 tattcaatta tattcttgta cttatcgtac ttttccttca gctccattat cggctgttcc
    34561 actgcaagaa gcgctatttt tgtttccaac tggtcaatct tgtctctggc ggctttctct
    34621 atttccttca tccgcttcag cgtctccatt agttttccct gaaccatgaa ggaattatcc
    34681 tctatcttct ttctttcctc aggaggcatc tctatatagt ctttgtcgtc cattggcttt
    34741 ccgtcccgga ggggaatggt cataagcccg tgctcgctct ttcttattac gaagccctgc
    34801 tctcttgcaa aaacattgaa ggactccata tattcggagc ttttcgcctg atactcctgt
    34861 ataatctgac ccttctgctt ttcatattct tccccttcaa aagcctgagg aacctttatc
    34921 ttaagatcct ttaagagcct tttcatatct ttttgaagct tgtaccccat tccggccggc
    34981 aggtttatac acttaggttt gtcaggattc ttgaaattat atacatagca ccaatcatta
    35041 ggtatgcttt catttgcggc tatcctgctt actatggacc tggtatagct gctccttccg
    35101 gtgcctgtta ttccggagac atatatatta tagccgggcc ttttgattgc aagcccgaac
    35161 tccattgctg cagcagccct gctctgtccg ataataccgt ttaacggatc caggtccgct
    35221 gtggtttcaa actcaaataa gccgcagtca attttgttgc tcagttcctc cggggccaat
    35281 tctctaaatc tgctcattca gcactctcct ctcaacttta atacattcct catatctatt
    35341 ttacctttgc atcatagaat gcaagggcta tgaatttggg gatcctcagg actctagtaa
    35401 ttctcctggg ctccttcaca agcctataaa accactccaa cccggctttc tgataaaact
    35461 caggagccct tttcactttg cccgcaagca catccagact tcctcccacc cccatggcaa
    35521 tctttacttt cagattatca ccgtgcttat atatgaattt ctcctgcttg ggggcaccca
    35581 atgccgctaa aagaatatct gccttgctgt tgtttatatc attaacaata tcattttctg
    35641 aatcttcttc gaaataaccg ttccggattc ccgctatcct caggctgccg tagctctcgg
    35701 taagctttat ggccgcctct tcagccacac cttccctgcc tcccagaaga tacacgcttt
    35761 tgccccggga atcggctata tccataagcc gcatcatcag gtcaaagcct gcaaccctct
    35821 cttttaaagg agtgccgtaa aaccttgaag caattacaac tcctattcca tccggaacta
    35881 caagatggcc gctgtttaaa acactcccga actccccgtc cccgactgcc gcaagcaaaa
    35941 tctcgctgtt aggggtaaaa accttgctgc atctgtcggt ttcaagaaat tcttccatta
    36001 aagccgccgc ttcatccata tttacattat caatttttat tcctactact tctacctcga
    36061 catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntagatac cgccaacttc
    36181 ctcaacgcgt tgggagccaa tatcaagggc gcaggcactg atgtcattaa aatcaaaggt
    36241 gttgaatccc ttggaggctg cgaatacagc gtgatacccg atcagatgga agcaggaacc
    36301 tatatgatag caactgccgc taccagaggc gacgttctta tcaaaaatat tatccccact
    36361 caccttgatg cagtttctgc aaagctcagg gaaatgggtg tggaaattac ggaatacgaa
    36421 gattccgtaa gagttaaagc cccctcaaga acaaaagcgg ttaatgttaa aacaatgccc
    36481 tatccgggtt tccctaccga catgcagcag cctatgagcg tactgctttc tgtttccgag
    36541 ggcaccggaa tcgtaacgga aagcatattt gagtcccggt tcaagtattt tgatgagctg
    36601 aaaagaatgg gggcaaaaat aaaaatcgaa ggcagggtag ccgtaataga aggagtttca
    36661 aagctgactg gtgctcccgt ttatgccacc gatctgagag caggggctgc aatggttgtt
    36721 gcaggcctta ctgcggaagg aaagactacc ataagcaaca tcagctatat tgacagaggc
    36781 tatgaaagat tggaggaaaa gcttataaac cttggtgcaa aaataaggcg catcgaggaa
    36841 aagtaggagg tatatatttg aagctgaaac aaagtttatc catactgctt gttttctgtt
    36901 ttttcattgt tattctaatg cccggtaccg cagcggcggc agcatcgggc accaaaatac
    36961 ccggcgcttt ctggagcctg gttacgcctt atcaaaatgc tgtggatgcc aacgacctga
    37021 ataatattat tgtttatggc aataaaataa tagagctgtt caatggaatg gaagaaaccc
    37081 agcaagtact ggaaatcata actccgcgcc tggatcaagt tgcaaaagct tacgaagccc
    37141 ttggaagatt cgacttggcc gttgaatatt tcagcaagta catccccaga gccgaaaagc
    37201 tgggctggaa ggatggagta acttatgcaa ggtcaaaaat caatacactg ggatttgata
    37261 ttgagcttta tgcaaaaacc acaaacttgg aaaagagtgt ttacttcggt tcaaaatatg
    37321 aacccaggtc aggggtatat tttggcagca attatgatac cgacccgcgg ataggaactt
    37381 atagctggaa tgatatcaaa aattattttc ccaggaagaa taccgcttac cttacatacc
    37441 tgcattggaa tgaggacatc aagtcctttg accgttattt taaggatgca aaagcaaaca
    37501 acatagccgt tcaactggcc tggaacattg aagattcaga aattgacaat gtacttaaaa
    37561 gcataaaaag ttatgattca tatataagag ccacggcaga ttacctggga aagcttaata
    37621 tacccgtatt cctcagattt gccggtgaga tgaatataaa agacaactgc aaggattata
    37681 attcatttat agcggccttc aggcatgttt cgggaattat gaaggccaag gctcccaatg
    37741 tggctatgat atggtctccc aatgatattt ccgccaaggg ccgcacctat gaccaatact
    37801 atcccggtga tgagtatgtg gactggatag ggataagcac atatacacag cgttacttcc
    37861 agggcaaaaa ggattgggga agccttcagg acactaatga cagtgtatat atgaccggaa
    37921 attatgcaaa tcccctggcc aagataaagc cgattatgga tgcctacggc ataaaaaaac
    37981 ccataatgat ttctgaaaca ggggtaagcc attacgcaaa gcttgcaggg gaagatttgt
    38041 ccccctgggc taaagtgcag ctgagacggc tgtacacgta cggccccatg atatatccgg
    38101 aactgaaggg catttactat ttcaatgcca ataatgacat aatcaccaag tatgatgatt
    38161 attcattata tggaaattca gcctttaacg agctttataa tgaattggta aacagcggca
    38221 attttatatc gtctgtcaac ggcaatccgg ccttcagata tgaagaggtt actgactaca
    38281 gtacccccgg cctcaagctt gagctttcaa cctatactgt cgttccaaag gttctaaacc
    38341 ccacggtgca atacaagctt gacggaaaaa tccatgccgt aaggactgta ataccttaca
    38401 gcatagaact tgacttatct gctgttccca aaggcgggca caagctgact gtggaaatct
    38461 acgacggaaa taacaaaatg ggctcaagag aatacacgct tacaataaac gatgacaatg
    38521 caaacctgaa aggaaactga aaatatttat taaccggagg cattatatgc gcaattacaa
    38581 cattctgatt acaaccatgc gccttgacat aggcggtgcg gaaactcata tagtagagct
    38641 tgccaaaggg ctttcccgtg agggctttaa tgtaattgcg gcatccgccg ggggcgtgta
    38701 cgaaaaggaa ctggccgaat ccggcatcaa ggtatatcac gtgcccctgg acaacaagaa
    38761 gcctcacaat gtgataaagg cttacttcaa gctgaagaat ataataaaag aaaatagaat
    38821 agacctggta catgcccatg cccgtattcc cggatttata tgcggcctgc tgcataaaag
    38881 aatgggattt ccttttgtga ctactgccca cggaacctgg aaaaccggtt acggattgcg
    38941 ctacataacc aattggggac agaaaaccgt ggcggtaagc gaggatataa ggcaatacct
    39001 tcttgataac tataagattg aggataagga tattaaggtc acaataaacg gaattgatac
    39061 cgataaattc tcctccggaa tcgattgctc cgatataatg gatgagctga atctcaaacc
    39121 ggaagacatg aaaacagtat atatcagcag atttgacagt gacagaagct atataataaa
    39181 caagcttctg gaagtaattc ccgagctttc ggaaagcatc ccaagcctta aggttattat
    39241 ggtaggcagc ggaaatattc tggactcgat aaaggagaaa gccagggaga taaataacat
    39301 aacagggcag aatacgatta ttgttacagg ctcccgtacc gatataaaca aattcaccgc
    39361 cctggcggat ttattcatcg gctttggccg ctcctcactg gaagcacttt ctgccgggaa
    39421 gccccttatt cttgccggca atgagggcta tataggaata ttgaatgaaa acagcctgga
    39481 tgatgcaatc aaatccaatt tttccggccg gggaaagagc aaagcagaca gtaaatcctt
    39541 caaggatgat attttaaagg tgctgagtct aagcggcgaa gagagaaaag ccctggggga
    39601 ctttggcaga agtgtgattg aaaacagtta ttccgtaaga aaaatggtgc aggacaatat
    39661 ggaagtatat gctcagctgc ttgaacataa ataaagggga caaacctatg tatgacgtta
    39721 tgatatcagg ttattacggt tttggaaaca gcggcgacga tgccatactg ctggctatta
    39781 ttgaaaacct gagaaaaata aaaaaagata tacgaattgt agtgctttca aaaaatccga
    39841 aggatactgc atcaatctac ggtgtggatt ccatagatcg ttttaacctt tttaaagtca
    39901 tcggtacaat gaaaaaaacc aggctttttc tgaacggcgg cggaaacctt atacaggatg
    39961 ttacaagcac acgttcattg gtgtattacc tgactaccat ttatcttgcc aagcttatgg
    40021 ggcttaaggt catgctgtat gcaaacggaa tcggcccggt tggcaggcgc accaaccgtt
    40081 attttacttc aaaaataata gacagagtgg atatcataac tctgagggag gaagcctcag
    40141 gcctggaatt gaagtcattg ggcgtcaaaa aacccgaaat ccgtgtcact gccgaccctg
    40201 ccttgggcct tgaaccggca ggctctgaag aaacagaaaa gctgctgcaa aaagaaggaa
    40261 tacccgcaga tacccctttg attggttttt ccgtcagaaa gtggagcggc tacgaggcat
    40321 acagccgcat tcttgcccaa gctgccgatt acatagagga aacctacaat gcgaaatcag
    40381 ttttcatacc cttgcatttt ccttcagacc tggcagcagc ggaggatatt gcatcaaaaa
    40441 tgaagcatgc tccctttata ctgaggaata tatatgaaac ggataaaatt ctcggaataa
    40501 tgaaaaagat ggatttagtg ctgggaatga ggcttcatgc ccttatatat tcggtcagcc
    40561 tttcagttcc tgttatcggg cttatatatg accccaaagt acagggcttc cttgaatatg
    40621 tgaatcagcc ctgggcaggc aatgtggcgg acttgaattt ggatagcctt aaaaagctta
    40681 ttgacgaagt atggagcagc agaaaagaaa tatcacagca gctggaaaag ggcaataggc
    40741 tgctaaagca aaaagcctat gaaaatgcgg agatagcaat aaagctcctt gaacaatagc
    40801 aaaaaaactg cgcagcaatt ttctactgct gcgctttgct caaattcttc acgtctacta
    40861 tttttgccac catttctatc atcctggtat ccatcacctg ctgggattcc ctgttgatat
    40921 ccaacctgcc gtttgccctg aagccgtatt ttgtctctat tcccggtacc ctgattgtta
    40981 tcagtacatt aaacctttca ggtttttccg ctattaccat ggtgccgtcg gcctttgtaa
    41041 gctggtctgt cgcaggcaat acttcaatgt cggtaatact ccccataggg ctgttggttt
    41101 tgacgtcata aatatccgca ccgatttcta tagcatccac agactgctgc ctgacttcct
    41161 ccaccagtac cttatactca atggtatcaa actttgcagc attctgatcc ttgtcaacca
    41221 taaaaaattt ataatacacc cccccgataa gtacagcaat aagcagcaat acacctatat
    41281 caacaatact gattttgccg aacaatcttc ctttttcatc caaaagcata tcaatccctc
    41341 cttactgccc aaccaaggtc attttggtta cgaagccctg atttgcatat cccttgcctt
    41401 taatgaaaaa cttcttgccc accttcactt cggcacctcc gattattata ctcgccggag
    41461 ttatattggc atcagcctct atcgttatga caacgtcata ggcatctggc acttcagcga
    41521 tgatatatct gccgttaatc aggtcttcat tgagcattgc ggcatccctt gaggacttgc
    41581 taacaaccgt ccccagttca ttccccctgg tgctgtcacg tatcgggtct cccggagtta
    41641 tcgcatccac aaagtccttg ttgacattgg ttatttcaaa gtcatacact acctttccgg
    41701 tatttaccgc ctgcctgctg ttgcctccgg aaaaaaacat atagccgccg gccgcagtca
    41761 atattatcag cagaattata atgagatcca taatatttat tttcccaaac agcctgcccc
    41821 tgtcatccca taaagccata tccttcatct tactcccttc tgtttttcct tgccacagat
    41881 tcatacaatt cttctatttt tacagccatt atttcggcag taaagctttt ctccatgatt
    41941 tcccttcccc tgtcgcccat tgtctttcgc agttcaggat catgaatcag cttaagtatc
    42001 gcatcagcca gcctttcggc atcccccgga ggaacaagaa atccgttata tccgtcctta
    42061 accacctcgg gtattccccc cgtatctgca gctacagacg gcttgccgac actcatccct
    42121 tcgattagtg acaggcataa ggcttcagat aaggaagaaa tcacattaat gtctataata
    42181 ttaaatattt catatatctc atccacgaag cccgtaaaaa ctactttatc ggtaagcccc
    42241 ttctgcttaa taagctcctt cagttcttct tccttcggcc cgataccggc aataaaaaac
    42301 ctgacattgt cattgtcccg ggaaataatt tcagctgcat ctataaaata tttgtggcct
    42361 ttgacctcgg caaggcgggc tacaattccg acaactactt cttcttcacc aattccccat
    42421 tttcccctgg caaggctttt ttctttatca tcaagtactt taaccgggtt tatgccccca
    42481 tatatcaccg atatcttatc atcgggcacc ccgttttcgg cgatattccg ttttacagct
    42541 tccgatacgg caattatgcc gtcactatac ctgtcgttta tcaaagctcc cgcaaacctc
    42601 ctgataccgg ttatagggct gtcaatacaa tgcttggtat taattattct tgctcctgcc
    42661 ctctttccgg caagccttgc ggacaacgat gcatgggtat gtacaatatc cggcttttct
    42721 gccctgaata tataatacaa aagatttacc gccctggcat cgtaggattt gtctttaatg
    42781 ccgtcaatag ctattacttc aaagcctaag ttttttatct tatctgtaag catgcttccc
    42841 cgcggaagca caacctttat ttcaaacttc tccttatcta ttgccccaag cagattaagc
    42901 agccagctgc ctgccccgcc tattttcata tcacttaata catgcattac cttaatcata
    42961 tgcccgacac ctcattatcc tccgtgataa gagccttggt tatcacaccg aaggccagca
    43021 tgacccaaaa agtcagaagc accctgttgt tgtaccatat attttcaacc attccctggg
    43081 caagataacc tgccatgccg gatgcaacgg ctatcatgaa agtggaaatg aacttgtctt
    43141 tggttttata gaagcctgcc agtaaagtct tgtaataaac cgctattatt gcaaaaaaca
    43201 tcaataggcc tgctatgccg gtctctatca taagctgtat gtatatatta tgggaatggt
    43261 gggcatatgc ggcgttaagt gaatacttgg gataaaccag cttaaagggc tccaggccca
    43321 atcctatgcc cgaaagccaa taatcctgcg ccagcttcaa agaccccagc catacggaaa
    43381 tcctgtagaa ggaagatgta tcgttcagat ttccgatact cgcaaacctg ttgattacag
    43441 aagccggcaa tacaaaggga agcatcagca gtccgactac tccaagaatt acgaacctcc
    43501 tgtccctgag cactgcaaac aaagcaaaag ccagcatcaa tccgacatat gcacctcttg
    43561 agtaagtata gacgaggcat ccgagcataa tagccgtcaa ccccaggatt gcaagctttg
    43621 ataaccaatt cttctgtccc cacagcatgg caattgcaat aggtatgatg agtaccaggt
    43681 attctcccag cacgttgggg ttttcaaagg tggagcctac ccgggccttt atgtcctcga
    43741 acagcgttgt atccacccac gcttcggagg tagctcccac agtcttcagc tgatatagtc
    43801 catacagaga tgcaatggtt gtagaaagca ccagcagtgc tgccaaaaaa taaagctgcc
    43861 gcctggtctt aattgtactt accaatatga aataaaacac aataaatgcc atatgtatca
    43921 ataatgcaag catgctgctg ccgggagtat atgatgtaat gaagctgtag agaagcacca
    43981 taccgaacaa tattacgaaa taatcaagaa ttgtgctcct gagcttcatg ctcttatccc
    44041 tgaaaagcct gataaaaaac gataccgcgg tgagcaggat cagccccagc agagccatgg
    44101 tcggagccaa aggaatgaac cccgcaacgg caaaaactcc tatctcatac ctccagagca
    44161 ccaatacaag agcggcaatt cctccgactg cgagagcaaa ggccatcggc tgcagaaaat
    44221 gccacatcgc tcccaatgct gttcctgtaa caatcgcagt aatttcaaat cttttgttta
    44281 aaactgcatc ttccgttctt ttatcataaa atgcatcgtg agatacgtta aaaaaaccaa
    44341 ataccgcctt gctgactata ctgccctccg ccagggcctt taccggcctg tttatcaata
    44401 tcagcaccgc accgcccagg aataatgcca atttcattat ttcactcata ccaaagagca
    44461 ttcctgcatt tctagaagta aaatccaaaa gcccaaaaac tgcggagaag gtaatgaaaa
    44521 gcagcccgta aaccctcgtg ctcatatcaa acaggttctc cggaaaaacc cttatggcct
    44581 tcagtaccct gctctcttct atagcaacgt ttacgctctc tgagaatttt tgtgaagcaa
    44641 atctgaacca ccttaaggga gatacaagga ttctgaatac aaggctttgc ctccatgtat
    44701 ccgcgatgtt ccattccctg tcaaaaaaat tcaatatgct gcttgaggct gcaagcttcc
    44761 cgaagccatg ccccaaacgc tgcataacaa tgaaaaacat gctgttttca tagtaaaata
    44821 caatcctgtc atatactttc aatatcattc cgaatatcaa gctttctttc attttttcac
    44881 ttcctgcatg ttcagcacta aacgccttct ctgattcata tttcacctta ttatattatt
    44941 attgccgcct aaatgtaaag taaatacaaa aacggacaac actaaagcca ccaaaaccca
    45001 acctgctttc cgtggtttat ccgtgcatcc ggtgttatcc gcgtatttgc ctgcaaggaa
    45061 taccccagtt ccctccaaaa ccccgtaacc tcgcaacctc gaaacctact gtaaagaata
    45121 agtacctttg attataagaa atgatttgtc ggcaagtgcc tttagccctc tgccttcgcc
    45181 cctggccgct atcagcatat ggttattgac aacagcctct ccgttcttaa gatcctttgc
    45241 cgcagtaata tccgaaaggc ctccgaaagc atcttctata gcggaaaact tcccggatct
    45301 gggtattatt tccgcacctt cccctgcaat aagcatctgg cctttttgca tctccactac
    45361 ttgaaacttc cccgccgcct ttgcggcatc ttttatttct tctctcagct tggctatctc
    45421 ctgctccttc agctccaatt cagtcttaag cttggctgtt tcctgtgcaa tcaggctgtc
    45481 aatataatac ttgatctgct ctgatttctg ttctacataa ctctgtgtaa cgatgggatc
    45541 ctgttccgaa cctggggtta ccccgtctgc agcgcttata acaacggcag agagcaccag
    45601 gataattgca gtcaatacta taatactcgt tttgcgactc ttactgttta ccatcccaat
    45661 tctccttttc tgaggcaatt gcgaaaaaac aggtgctttt tttcacatct gcccataaaa
    45721 tccaataaaa tataatatct tcaaacttag tatatatttc gtcaaatatc gcaaaactcc
    45781 tgtatatgtt acaaatttgt aatttttatc ttacctgccc gttaccatat atgatgtgct
    45841 ttgtggatac gttaatatat acggcggcat gcgtcaggct tttcacctgc agctcagagg
    45901 ccgggttaat atgtcaataa ttcttgaagg cacctttatt tgctcaatac cgaatcaatt
    45961 atatcaattg ccctgtcaat atattcctct tgaatgttca atggcggaag aagcctaact
    46021 acatccgcgc cggctcctac aagaagcagt cccgaatcaa tgcattcatc gataaatttt
    46081 ttggtatcga ttgaaagctg gagccccaaa agcagtccaa tgccgcgaat gctctttatc
    46141 gagccgtgtt tttccttcaa agcctgcagc ttgccgacaa aatacttgct catatgctca
    46201 acattttcca gcactccgtt ttgcagaagc tcctcaagta ttgtcaacgc tactttgcag
    46261 gcaagaggat tgcctccgaa ggttgaaccg tgatctccat aagctatgca ttccgctgcc
    46321 ttccctgatg caacaaccgc acctatcggg aagcctcccc ccagcccttt tgcaatacat
    46381 accaaatcgg gtttaacacc gaacttttca aaggcaaata aactccccag ccttcctatc
    46441 ccgcactgca cctcgtcgaa tataagcagg gaatcgtatt tttcacagag tttttttgct
    46501 tcctccaaaa atgatttatc agctgccaat attccccctt ctccttggac cggctctgca
    46561 ataattgcgc atacattgga attcatttct tttctcagct ggccggcatc attgaggtca
    46621 acgctcttta ctcccggtat cagcggcatg taatccttct gatatttctc ctgtccggtc
    46681 acggaaagtg cccccagggt cctgccgtgg aaagagttat tcagataaat tatttcatgc
    46741 tttaaggcac cccctttaag cttgccgtac ttcctcgcaa actttatggc agcctctatt
    46801 gcttccgttc cgctgttgca gaaaaatgcc ccttcaagcc cgcttaattc acacagcctg
    46861 tcagccagct ccatctgaag ctcattccaa tacaggttag acacgtgcat cagcttgcag
    46921 ctttgttccc ggatggcgtt tataatggca gggtggcaat ggcccaagca gtttaccgct
    46981 actcccgaca caaaatccaa gtattctttc ccattaatat cataaatcag gcatccttta
    47041 ccgctgactg cggtaacatc aaaccgtgca taagttttca gtatcttttc cttagacatt
    47101 ttcctctcct cctataataa ttgtccctgc ctctttgcca tggaaaagct ccagcagaat
    47161 atggggcttc tttccgccta taaggcatac cgactttact cccttttcaa tagcttcaat
    47221 acagcatttc atcttgggta tcattcctcc tgatatggca ccggcctgta caagcttttc
    47281 aatttccgca gttgtaatgc tgctaatcag gctgtcggga tttccaatat ccccgaacag
    47341 cccgtcaaca tcggacaaat atacaaggct gtccgcaccg atagaagcgc cgatagcagc
    47401 tgccgcataa tccgcattga tattataagc tttgccttct ttgccttccg ccaccggtga
    47461 tataaccgga acatacccca ggctcaaaag atccgtcaat atttttccgt taaccttggt
    47521 tacttcgcct acatagccta tatcaatatt actgccgttg tcattcaggt acatcctttc
    47581 cgcctcaagg agctttccgt ccctgccgct tatacctacg gcatctgctc cctttccaat
    47641 cagttttgag gtcagctctt tattgaccct gccggacaac accatttcaa ccacttccat
    47701 ggcagcctca tttgttttcc tcagcccttt tacaaattcc cccgcaatat tcagcctgcc
    47761 aagcatcctg tttatatcag gcccgccgcc gtgaacaatc acaactttta caccggcatt
    47821 tctcaagatt gccgcatctt ccagaaatgc gtccgttgct tccttgtcct tggtaatgct
    47881 tcccccatat ttaataacaa ctgtcccgcc taaatccatt cggccttcac tcctaattct
    47941 taatggtact tattatacat cgtattggat ttttatgcaa tattatttat gtatttttat
    48001 tatttctatt gcataaataa ccgtccccat gtataatatt taaaaagcgc aatattgagg
    48061 tgattaatat gataaacgca ggtgtcgttg gttcaacagg ttatgcgggc tatcagcttt
    48121 cgatgatatt gtcccagcat aaaaatgtat ctatagtttt tttatcttct cataattatt
    48181 ctaatttgag attcagtgat gtctatggaa atctgaagta tttattggac aatatgtgca
    48241 tagatatgca agatatagaa agcaaacttt caaaaatcga tgtattattt cttgcactcc
    48301 cccacggcca atcctttgac ctggtggaaa aagcccttgg cctcggagtg aaggtcattg
    48361 atattggagc cgattatagg cttcgggatc aaaaggtcta tgagacgtgg tacggtgtaa
    48421 cccataagct ccccggccgc ttgaaagatg ccgtatatgg ccttcccgag ctttaccgtg
    48481 acagtatttc aaactcaagc cttgtggcaa atcccggctg ctatccgacc gcaagtattc
    48541 tggctcttgc gcctttagta cacaccggta ttattgataa cacctcaatc attatcgatg
    48601 caaagtccgg agtctcaggg gcgggcagaa gtgcaaacgt ctcatcactt ttctgcgaat
    48661 gcggtgaatc tgtcaaggca tataatatcg caacccacag gcatactccg gaaattgaac
    48721 agcagctttc cgatgctgca ggcgcaaaaa ttaccctcag cttcacacca catcttgtgc
    48781 cgatgagcag gggaatactc gcaacttgtt acggcagcct caagaaggat atttcgcaga
    48841 gcgaactcta cgacttatac agaagctatt ataaaaatga gaatttcatc agaattaccg
    48901 actccctgcc ggaaacaagg tttgtaaaag ggtcaaatat ctgtgatatt gccgtaaggg
    48961 tggatgagag gacaaacagg gttatagcaa tcagcgcaat agacaatctt ataaaaggtg
    49021 ccgcagggca ggcggtgcag aacatgaata tccttttcgg gctatatgag catacaggct
    49081 tggatatgct gcccaatatg ctgtaaagga gaatgtataa atgaagatta ttaaagatat
    49141 gaccataaca gatgtaccgt atttcaaggc aagcggtgta agctgcggaa taaagaaaaa
    49201 cggcaataag gatttatgcg taatatacag tgagaaggat tcggttgctg ctgctgtttt
    49261 tacaacaaac aaggtaaagg cggcgccggt aattcttgat atgaagcata ttcagtcaaa
    49321 aagcattcgg gcaattgttg caaacagcgg caatgcaaat gcctgcacag gaaaacaggg
    49381 tcaaaaggat gcaaatacaa tggcagagct gactgcctcc ctcctgaata ttccctctga
    49441 ctcagttctg gtagcgtcaa ccggagttat cggcgttcct ctcccgatga aaaatattga
    49501 ggcaggaatc agaaatgcat gcaggaatct gtcctatgac ggcggtaagg atgccctccg
    49561 ggcaattatg accaccgaca catgtgaaaa gtcagtaacc gtcgaagttg agcttgaagg
    49621 taaaaaggtg ctgctcagcg gtattgcgaa ggggtcaggc atgatacatc ccaacatggc
    49681 tacaatgctt gggtttatca ccactgatgc aaatatatca aaggaaatgc tccaggaggc
    49741 cttgagcgaa agtgtaaaga aaacttataa tatgatatcg gtggacgggg acacaagtac
    49801 caatgacatg gtaataatgc ttgccaatgg agccgcggat aacaaactga ttggggccag
    49861 ggataaaaac tttgatgctt tcatgaaggc ccttgacctc ctgaataccg agcttgcaaa
    49921 aaagattgcc cgtgacggcg aaggcgccac aaagctgatt gagtgcactg tctcaaacgc
    49981 agtttcctat gccgatgcat gcatactttc aaaatcagtt atcggctcaa atttgaccaa
    50041 agccgcattt ttcggtaatg atgccaactg gggaaggatc ctgtgtgctc ttggctactc
    50101 cggcgggaat ttttccccgg ataaggcgga tatttatttt gaaagcgagg caggctgcat
    50161 ccagcttgtg gccgatgggg agcctcttca gttttgtgaa gaaaccgctt ccgcaataat
    50221 ggacaaagaa tatgtaataa taaaagtgga tttaaaggaa gggggatatt cggctactgc
    50281 ctggggatgc gaccttacct atgattatgt aaagataaac ggaagctaca gatcataaga
    50341 gacagaggga cgtttcgaga cacggggacg tttctcctgg ggacataggg atgtttcccc
    50401 tgtctcacaa tagaagaata atgcgtcgca ttcttcgggt acgaggtacg aggcgcgagg
    50461 ttcgaggttt aggg
//
LOCUS       DAOOAB010000014        49915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_856_length_49915_cov_16.521340, whole genome shotgun sequence.
ACCESSION   DAOOAB010000014 DAOOAB010000000
VERSION     DAOOAB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 49915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49915
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_856_length_49915_cov_16.521340"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1378)
                     /gene="glmM"
                     /locus_tag="PKU88_04785"
     CDS             complement(29..1378)
                     /gene="glmM"
                     /locus_tag="PKU88_04785"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPX68633.1"
                     /translation="MGRMFGTDGVRGIANKELSPALAFNLGRIGAYVLTEETHKKPRI
                     AVGKDTRISGDMLEAALIAGICSMGAEAVSLGVLPTPAVAYLTRHMGLDAGVVISASH
                     NPFEYNGIKFFNGEGYKLSDQLEDRIESLMLNPENGIAYPTGADIGKKVEVDNAVHGY
                     VDFLKKTIDVNLRGLKIAVDCANGASYIAAPSVLADLGAEIAVINNKPNGKNINVKCG
                     STHPEGLQKLVVESGAHIGLAFDGDADRLIAVDNRGNIVNGDHMLAIFARHLKGKGML
                     KKDTVVATVMSNMGLDIALKREKCNLVKTKVGDRYVLEEMVSMGYSIGGEQSGHIIFL
                     DHNTTGDGLLTALQLLAVVKETGKKLSDLASFMKELPQVLVNAKVKNENKDKYMKDEA
                     IAGMITGLEKKLKGRGRVLIRPSGTEPLIRVMLEGANETEIKEDAEALAKLIEEKLN"
     gene            complement(1479..3170)
                     /gene="argS"
                     /locus_tag="PKU88_04790"
     CDS             complement(1479..3170)
                     /gene="argS"
                     /locus_tag="PKU88_04790"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPX68634.1"
                     /translation="MNFKVEIAKKINTLVDMEFDKVMELIEIPPQTNMGDYAFPCFQL
                     ARVMRKSPNMIAAEIAGKVQADSIVEKAEAVGGYVNFFINKKEYIRIMIQEILEKGEK
                     FGSSEEGKDKNVIVEFSSVNVAKPFHVGHLFNTILGSALEKVFRHSGYNTIRINHLGD
                     WGTQFGKLISAHKRWINEEALEKEPISELFRIYVKFHEEAEKDPALEDEARAYFKKLE
                     EGDSEVTILWQRFKDLSLREFSEIYEMLNVEFDSYAGESFYSDKMDEVVQILREKKLL
                     KDSNGAKIVDLEQFSIPPIMILKSDGATIYATRDLAAAIYRKRTYDFYKNIYVVGGTQ
                     SLHFNQIFSTLGLMGFEWSEDCVHIGHGLVKFADKKLSTRKGDVVFAKDVMNEAVHKT
                     MEVIEHKNPNLENKEEVARKVGMGALLYTFLKNNREKDVVFTWEDALNFDGDSGPYVQ
                     YTYVRGRSILQKAGYVPDTADLTFLSTEDEFQLVKLLGAFKDSVKEAVERYEPFVVVR
                     HLTEIAKAYNKFYNTHPILNADENVKNARLQLTKAVGIVIKTGLSLAGIETPDNM"
     gene            complement(3279..3815)
                     /locus_tag="PKU88_04795"
     CDS             complement(3279..3815)
                     /locus_tag="PKU88_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPX68635.1"
                     /translation="MAHEIIMAGFGGQGVMAMGKILVEAAMEEGKNVSWLPSYGPEMR
                     GGTANCSVIVSDEPIGAPIVTEATAAIVMNRPSLDKYENTVVPGGALLINSSLIDKKA
                     TREDIKVYYVPANEIANELGTDKVANIVMLGAYLQSSGAASFDSIMNIITHIFAGKKA
                     SVIPLNKEALLRGAACIK"
     gene            complement(3815..4561)
                     /locus_tag="PKU88_04800"
     CDS             complement(3815..4561)
                     /locus_tag="PKU88_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX68636.1"
                     /translation="MAVVFEKTRGLAEKPFHYCPGCTHGIIHRLVAESLDELNLIDDA
                     IGVAPVGCAVFAYDYFNCDMQEAAHGRAPAAATGIKRVNPNAAVFTYQGDGDLASIGA
                     AEVVHAAMRGEKITTIFVNNAIYGMTGGQMAPTTLVGQKATTAPYGRDENHAGMPLRV
                     SEMLATIPKAAFVERVSVHDVKHIIQAKKAIKKAFQLQMEGKGFTLVEVLSTCPTNWG
                     KTPIEALKWLQENMIPYYPLGNFKNIEEVK"
     gene            complement(4561..5628)
                     /locus_tag="PKU88_04805"
     CDS             complement(4561..5628)
                     /locus_tag="PKU88_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPX68637.1"
                     /translation="MAKELWKGNEAVAEAAIKAGCRFFFGYPITPQNEIPEYMAMKMP
                     QIDGCFLQAESEVAAINMVYGAAGAGARVMTSSSSPGISLKQEGITYIAGAELPCVIV
                     NMVRGGPGLGGIQPAQSDYFQSCKGGGHGDYRLVVLAPASVQEAVDLTQEAFDIADQY
                     RNPVLILGDGMIGQMMEPVEFTEVKKRKLPEKTWATVGTKGKRKTNVINSLFIDPADC
                     ERHNFHLFEKYAEIEKNEIKVEEYNLEGAEIVFAAYGTTARIVKNAINSLAKEGIKAG
                     LIRPITLWPFPAKAFEKAAEQAGVKAFMSVEMSMGQMVEDVKLSVNGKKPVYFYGRTG
                     GMFPTPKAIAEEAKKILGGAK"
     gene            complement(5648..5854)
                     /locus_tag="PKU88_04810"
     CDS             complement(5648..5854)
                     /locus_tag="PKU88_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPX68638.1"
                     /translation="MAKVTFDENRCKGCELCTTVCPVKIVVMDKDRINIKGYHPATVK
                     EMDKCTGCASCARMCPDVVIKVER"
     gene            complement(5904..6578)
                     /locus_tag="PKU88_04815"
     CDS             complement(5904..6578)
                     /locus_tag="PKU88_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX68639.1"
                     /translation="MYNDRRVRIITGHYGSGKTEFAVNYAIALAEAGKKTAIVDLDIV
                     NPYFRSREREPLLVEKGIRVISSSVKGIFGDLPALSPEIYSVLQDTSYEVVLDVGGDK
                     VGARALGRYHEYFDREPYDMFFVLNANRHQTSTVDGAISYLRSIEHGSMQKVTALVNN
                     THLCGNTTVEDIMRGQKLCEDVSKVLGLPIKYVVVEKKFVDVLPKGLRGDVLPINIYM
                     KKPWED"
     gene            complement(6606..7679)
                     /gene="buk"
                     /locus_tag="PKU88_04820"
     CDS             complement(6606..7679)
                     /gene="buk"
                     /locus_tag="PKU88_04820"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPX68640.1"
                     /translation="MQEYRLLIINPGSTSTKIAIFDNEKPVLEETLRHSNEELAPFGA
                     IIEQYEFRKNVILDTLNANGINITKLNAVVGRGGLLKPMVGGTYHVNGKMIEDLRGAV
                     MGQHASNLGGIIAHEIASQLGIQAFIVDPVVVDEMDEIARISGMPDLERISIWHALNQ
                     KAVGRRAAADLNRKYEDLNLIIAHLGGGISVGAHRKGRVVDVNNALDGEGPFSPERAG
                     GLPVSGLIKLCYSGKYTLDEIKKKITGKGGLVAYLGTNDGREVVRMIGEGSKKAELVY
                     RAMAYQVAKEIGACAAVLEGKVDAVCLTGGLAYDKLLTGWIKERVEFIGEVKIYPGED
                     EMIALAEGGLRVLRGEEAAREYK"
     gene            complement(7742..8644)
                     /gene="ptb"
                     /locus_tag="PKU88_04825"
     CDS             complement(7742..8644)
                     /gene="ptb"
                     /locus_tag="PKU88_04825"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HPX68641.1"
                     /translation="MIRTFKETIESAKAKGPKTIAVAVAQDPEVLSAVNAAKKLGIAE
                     AILVGDREEIIKASEECGIDIGKYEVIDIKDKNEASRKAVEMVSGGRAHIVMKGIVDT
                     AIVLKAVLDENIGLRTGNVLSHVAVFEVPGYDRLFYVTDPAMILAPSLIQKKQIIENV
                     VQVANALGNNNPKVAVLAAVEKVNPKMQATLDAAELVKMNESGELKGCVVGGPFALDN
                     AISVEAARHKGVTHPVAGLADVLLVPYIEVGNVLYKSMVYYAGSKVAGILVGAKAPVV
                     MTSRADSDEAKLNSIAIAVLMAGK"
     gene            complement(8645..9727)
                     /gene="buk"
                     /locus_tag="PKU88_04830"
     CDS             complement(8645..9727)
                     /gene="buk"
                     /locus_tag="PKU88_04830"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPX68642.1"
                     /translation="MSREYILAINPGSTSTKAAVFKGEENILQKNLSHSAEEVTKYEK
                     ITDQYEYRQKMILDWLKESDISTGSLAAVVGRGGLLKPMPGGTYIVTDAIIDDLRSAV
                     QGEHASNLGAIIARGIADTEGIPAFIVDPVATDEMDDIARISGMPELERPALVHALNI
                     KAVGRRAAKDMGKEFTDVDFVIAHLGGGISVSPVRKGKIVDVNGANDAGPFSPERAGG
                     LPVGNIVKMCFSGKFTQAEMKKKAVGSAGLVGYLGTNDARDVVKMIEAGDRKAALIFD
                     AMAYQIAKEIGAMATVINGKLDAIILTGGLAYSDLLCREITKRVEFIGPVLIYPGEDE
                     MQALAEGALRVLKGAEKAKIYEEEVK"
     gene            complement(10016..11731)
                     /locus_tag="PKU88_04835"
     CDS             complement(10016..11731)
                     /locus_tag="PKU88_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HPX68643.1"
                     /translation="MKKDIELILNSTHDAMIGVDTDGIITLFNKAAERLTGIEAKDAM
                     GKYVADVIKDTRLPYILETGESELNRQQDLGNIKIITNRMPVKDKKGRIIGAVAVFRD
                     ITEIIKLAEEITNLNEVYSLLEAIINATQDAISVVDQNGLGVMINPAYIKITGLSEKD
                     VIGKPATVDIAEGESIHMQVLKTRKPIKGALMKVGPARKEVLVNAAPIIVNGELRGSV
                     GVIHDMSEITKLSNELKNAKNIIRSLEAKYTFEDIIGSDPLLLSAIDKAKKAAEIPIT
                     VLLRGESGTGKEIFAHAIHNESERKFRKFIKVNCSAIPETLLESELFGYEEGAFTGAK
                     KGGKKGLFEEAEGGTIFLDEIGEIPHSTQTKLLRVLQEKEIVRVGGTKSISIDVRIIA
                     ATNVDLEVAIESGRFRADLYYRLNVLPITIPPLRLRKGDIYDLSIFFIKKFNQQYGRN
                     VRDITQQAVSRLKEYNWPGNVRELDNFIGRAIINMKASDTVIQENHLPKLEGFLDAGD
                     NPDARMKGRGSISMTLDSVVEKAEKEHIAKVMKECGNNKTKAAKNLNISLRSLYYKLE
                     RYGLE"
     gene            complement(11871..13502)
                     /locus_tag="PKU88_04840"
     CDS             complement(11871..13502)
                     /locus_tag="PKU88_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HPX68644.1"
                     /translation="MHFEVNNLKQPVRDFPFDNDADMVRFLAAEKLRIREKDISSLRI
                     LRRSVDARDKKDILLVYSAEVEINESTSPSRYLRVKDVARVNERAEYEEENKLVYGSR
                     PINNRPIIIGSGPAGLFAGLVLAQHGYSPLILERGSDVDKRAEAVNTFFRQGVLDEES
                     NIQFGEGGAGTFSDGKLTTRIKDRRISRVLNTFVEEGAPKEILYQAKPHIGTDILKTV
                     IKNMRKRITKLGGEVRFLSKVTDILVEEGRAKAVIVNGKSAIESNIIIAALGHSARDT
                     YGMLLERGVRIIPKPFSIGVRIEHLQSMIDEAQYGSFAGHPRLGAADYLLTDRSSSTG
                     RAVYSFCMCPGGQVVAAASERGMLVTNGMSEYKRDRENANSALVVSVSPDDFKSSHPL
                     SGVEFQRIWEGKAYKLGGGNFHAPVQLAEDFLKGKASRAAGRVKPSYEPGVKPCDLHL
                     CLPEYVTLSMKEALRDFDRKIKGFASSNAIMTGVETRTSAPVRIVRKDNMESESVENL
                     YPVGEGAGYAGGIVSAAVDGIKAAERIIQNFKPIK"
     gene            complement(13515..14741)
                     /locus_tag="PKU88_04845"
     CDS             complement(13515..14741)
                     /locus_tag="PKU88_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPX68645.1"
                     /translation="MNNKLINKDHTIRILSVMLALLLWFYVITEQNPEITKDVTVPIR
                     LINTVFLEKSNMVIVNDTDNFALTMRIKGKKDVLDKLNANTVDAYADLAGHNLKGENF
                     LKINVDGIPEGVNIITRSMDSLKIMLESKISVQRSVKLNIMGNPTHGMAIMMHSLTPS
                     DVVITGAESKIAMIHEVRVDVDIASANAEVRKVLPVRVLDKTGKDIQDITVEPGNVEV
                     SIPIENTKWVSLSMDISGQPAAGFVVSGASVQPEEVLVTGKQQILEGITSIKTEKIDI
                     TEVKAAVSKEVRLVIPEGLEIVNADEIVKVSVNIEKIATSEIIVKDLEYVNLPEDLEL
                     VSIQGDIKITLRGAESRIADAPRTVKYYVDLKDAVEGSNVLNVLWEAPQGIEVLEVSP
                     NQAAVVLISKAKLQEP"
     gene            complement(14734..15552)
                     /gene="cdaA"
                     /locus_tag="PKU88_04850"
     CDS             complement(14734..15552)
                     /gene="cdaA"
                     /locus_tag="PKU88_04850"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPX68646.1"
                     /translation="MDEFKELLPYLKMWNTYVDIGIIAIIIYKAMNLIRETRAEQLIK
                     GIAMLLIATKLSEVLGLHTVYWLLKNAMTVGVIAILIIFQPELRRALEHIGRGRFFIK
                     GELLEHEIDSMLDEVCVAVAQLSKSKTGAIIVIEQETGLNEYIETGTKLDSVVNNEIL
                     INLFVPNTPLHDGAVIIRRNRIAAAGCFLPLTQSQNLSKELGTRHRAAIGITEISDSM
                     VIIVSEETGVISFAKMGRLSRYLDTKSLREIIKNSIKARENKQPIWMKWGVKNE"
     gene            complement(15684..16469)
                     /locus_tag="PKU88_04855"
     CDS             complement(15684..16469)
                     /locus_tag="PKU88_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPX68647.1"
                     /translation="MNIIQAIILGLVQGLTEFLPISSSGHLVLFQKIFGINEPTLVFD
                     TMVHVGTLAAVMTVLRKEILSLLKRPFQRLTWLLIAGTIPTGIIGIAFKDSFEAMFQS
                     GSTLGFEFLATGIIILFAGRLNNGGKHIEETTFLDAAFIGILQGAAIMPAISRSGLTI
                     SGALMRNLDREFAARFSFLLSIPAILGAALFQAKDILEAGSSGIINAPIVAGTIAAAA
                     AGYFSVRFMIMLVKKGSMKYFSYYVFIIGALVIIDQYITHLFF"
     gene            complement(16655..17608)
                     /locus_tag="PKU88_04860"
     CDS             complement(16655..17608)
                     /locus_tag="PKU88_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPX68648.1"
                     /translation="MWNDNSFLKKEMDAVEDTIKNSLRTRNKTVKEALVRFAESGGKR
                     LRPAFTILGGTFGQYDSNTIVPIAAALEIIHMATLIHDDIIDDAKLRRGNETVHSVLG
                     KDVAVYSGDFLLTRAFMLVADYADIDMLKQIAKASAYICDSEIAQNEQKFDTNVSVKQ
                     YLKRIGGKTAALFGISLYMGAYKAGCPRKLVNRLGVLGNNIGMAFQIVDDILDFTGNQ
                     AKVGKPLLRDAAQGIYTLPVLYALHSEYREEAIKAINKTQEEKGANLYEVLDASKAIE
                     RSKNIAEKFIGKAMKIAGELPESIGRDIIMEIIDEQLERKF"
     gene            complement(17598..18125)
                     /locus_tag="PKU88_04865"
     CDS             complement(17598..18125)
                     /locus_tag="PKU88_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPX68649.1"
                     /translation="MSRTKKMVLLGILVSQALILNLVERLIPVPVPVPGIKLGLANAI
                     SLIAIILFGPKEALAVVGMRTFLGSVFGGGISSFIYSFIGGTISTMAMAIMYRKFRTL
                     FSLPSISVIGAVFHNIGQILVASLIIENAKLFYYLPALLISAVITGLFIGFAVQYTLK
                     PMSHVLGIGSDTNVE"
     gene            complement(18146..18529)
                     /locus_tag="PKU88_04870"
     CDS             complement(18146..18529)
                     /locus_tag="PKU88_04870"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPX68650.1"
                     /translation="MKIKKGDLIIIVILVAALISWLVINNPGKPEDERLIVIETNGDI
                     YREIPVETGMEQQEIHIQFENGKYIDIIVDENGAYVKDVICPDKVCQKTGLVSRVGQS
                     IVCLPNRVVVYIEGRAESEIDGISF"
     gene            18760..19818
                     /locus_tag="PKU88_04875"
     CDS             18760..19818
                     /locus_tag="PKU88_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPX68651.1"
                     /translation="MILDAKLRLTALALLSVLLLSGCQKPFPSASAKPLSKTGFLLGT
                     IVEITVYDKKDEALLDKAFERIQEIEGRMTINNAENSEIISLNQASGKNEVKLSPDTY
                     YVVERGKEYSHKSGGLFDITIGPVVKLWNIGTDYAAVPEKNKLEEAAGLVDYSKLSLN
                     PGNHTAFLEEPGMQVDLGSIAKGYAADEAARILKESGVEHAIINLGGNVITIGGNPNG
                     NPWRIGIQTPSNPRGDFLGIVQIQDQAVVTSGTYERYFEENGKIYHHILNPFTGYPVE
                     NNLSSVSIITGKSIDGDGISTTVLLMGLEEGMKLVESLENVEAVFITNDKGVYISSGL
                     KNNFKITNSDYKYRNNGE"
     gene            complement(19845..20138)
                     /locus_tag="PKU88_04880"
     CDS             complement(19845..20138)
                     /locus_tag="PKU88_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68652.1"
                     /translation="MHKYVKRLKRRAESGDESSKDKYEEIMECLNTMDDDRVANCIIL
                     MKKHIPELWNQSALLLKKYIIEESRSLEFLSKAEVELIEFLRDYLRDEGDDRH"
     gene            complement(20201..21802)
                     /locus_tag="PKU88_04885"
     CDS             complement(20201..21802)
                     /locus_tag="PKU88_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX68653.1"
                     /translation="MTGFLLGYTVINLILGTVLFMYRGNELAVILLIIAMAATGLLIL
                     AKLNSISSRIGNSIEEINKGQLNKNVKKTGIIMFDSIASAMNEFLLKIRSLIGSFDDV
                     SKRIMKDAHEVEKQAEAIRYSSGEIASTIQSVAESVSNQAAYTHNMMEMVQDFTKGAE
                     DISENAETSLKIAKETKGTIEDSFGKFSDIKFKIQESKEYNKKVLEALDNLDNKIRAI
                     DTITEAVESIAAQTQLLALNAAIEAARAGEAGRGFAVVAGEVGKLADDSSQSAKEIKQ
                     LVDGITSQIGELSVNIKDEASAIDKNLKFAEEVLSKSDVINSTLTDNMKAAEKITMLT
                     KEQMKNIGSIEKEIQKVNDVTQQNAAVSEEIGASTEEQLATIEAVHGHIVMLLERLEE
                     SNAIIGNFMKGFVLTDAIKEKINKTQELLREIIQKENIMALDKNSAERYIKEQQKKLE
                     YIELIVIIDNRGYITGATTELPEFVRDCSSKPYFTNAMKGNTYVSKEYISCASGNYNI
                     SVSMPIIVNGELKGVINADININEN"
     gene            complement(21831..23429)
                     /locus_tag="PKU88_04890"
     CDS             complement(21831..23429)
                     /locus_tag="PKU88_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX68654.1"
                     /translation="MGLLSLYLIINIVFGAALYLMRSSQLAAAALIILMTASGFVFIA
                     RLSSISGRIKHNIEEISKGQLNKNVKKTGIIMFDSIASATNEFLLKIRGLIGSFGDVS
                     KRIMKDAHGVEKQAEAIRYSSGEIASTIQSVAESVSNQAAYTHNMMEMVQDFTKGAED
                     ISENAETSLKIAKETKGTIEDSFGKFSDIKFKIQESKEYNKKVLEALDNLDNKIRAID
                     TITEAVESIAAQTQLLALNAAIEAARAGEAGRGFAVVAGEVGKLADDSSQSAKEIKQL
                     VDGITSQIGELSVNIKDEASAIDKNLKFAEEVLSKSDVINSTLTDNMKAAEKITMLTK
                     EQMKNIGSIEKEIQKVNDVTQQNAAVSEEIGASTEEQLATIEAVHGHIVMLLERLEES
                     NAIIGNFMKGFVLTDEVKEKVKETQTAVNELVKNEGILRMDKNAVEKLFREHQQKYNF
                     IEFMGLIDSKGYLTFSTVEVPENHRDASAKPYFIKACQGEAYVSEEYISVATNNYNIS
                     VTVPFQRGGIFGGLIIADININEN"
     gene            complement(23560..23751)
                     /locus_tag="PKU88_04895"
     CDS             complement(23560..23751)
                     /locus_tag="PKU88_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68655.1"
                     /translation="MTASIRLDNNEAKQTKQIIRFFKKGVKIRYNKKLTGKVRLYISE
                     KNSVKNISDILSLYENRLN"
     gene            complement(23837..24700)
                     /locus_tag="PKU88_04900"
     CDS             complement(23837..24700)
                     /locus_tag="PKU88_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPX68656.1"
                     /translation="MKPYKTNPMIAERIWGGRRLCSYNKNVGDKNAGESWETVSPEGE
                     IPVLIKLIDARETLSVQVHPDDEYAIRVENARNGKSEAWIVLECEEDSFIIYGFNRMV
                     QKKELRELLEMGLISGVLNYINVKKGDCIFVPAGTVHSIGSGILAYEVQQPSDLTYRI
                     YDWNRTDSRGKGRELHIDKALDVINYSSGLPCVFNVYDSLEDGIINIIECEYFALSFR
                     FINKEECHFYEPGKFRAITAVSGNLLLRYEDFQLSVKKGDTCIIPEEYDREVRVEGLG
                     AAEYIVAAGSN"
     gene            complement(24869..26470)
                     /locus_tag="PKU88_04905"
     CDS             complement(24869..26470)
                     /locus_tag="PKU88_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX68657.1"
                     /translation="MAGFLSGYVLVNVILGAALFMLRSNVLAVIILMLLIISSGIIFM
                     TRLKGIAVRIGDNIEVINKGQLNKNVRKMGIDMFDLISSRINDFLFKVRALIASFGVV
                     SKKVMKDAHEVEKQAEAIKYSSSEIATTIQSVAESVSKQAAYTHNMMEMVQNFAKDAE
                     DINANANTSFNVAKETKDIIEDSFRKFEDIRIKIQESKEYNKKVLQALDSLDEKLRAI
                     DTITEAVEGIASQTQLLALNAAIEAARAGEAGRGFAVVAGEVGKLADDSSQSAKEIKQ
                     LIDGITGQISELSVNIKDEADAIDKNMVYATEALSKSDIINDTLVSNMQAAGKITTLT
                     KTQLTSIDSIEKQIEMINDLTQQNAAVSEEIGASTQEQLATIETVHGNILQLKERLEE
                     SDSIISNFMKGFQLNDSIREKIKKTQELVKEITSRGDLFSSDKSSVQKYLNEQQSRYE
                     YVEFMALINSSGYYELATQDVDESLKDCSAKPYFTRAIQGEEYTSEEYISVITNNYNI
                     TVSMPVRQNGTIKGVLMADININEN"
     gene            complement(26705..29149)
                     /gene="leuS"
                     /locus_tag="PKU88_04910"
     CDS             complement(26705..29149)
                     /gene="leuS"
                     /locus_tag="PKU88_04910"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPX68658.1"
                     /translation="MPYSKDIDRKWQKRWEETGLYKFNPQNADKKLYCLEMFSYPSGA
                     NLHVGHWYNYGPTDSWSRIKRMQGYEVFHPMGFDAFGLPAENYAIKTGIHPWDSTKNN
                     IATMEAQLREMGATFDWDYEVVTCDPSYYKWNQWLFLKLYENGLAYRKEAPVNWCPRC
                     NTVLANEQVVEGHCERCETEVIRRNLTQWFFKITAYAQELLDKLDEIDWPEKTKKIQR
                     NWIGRSEGSEIEFKIYRKDLSFKTFTTRIDTLMGVTYVVLAPEQELVDVITAPEQKEA
                     VEAYKDFARKASEIDRLSTTREKTGVFTGAYAVHPISGEKIPVWISDYVLATYGTGCV
                     MGVPAHDERDFEFAEKFGLPIPRVIKGVGDVDDKLPFVEYGVLVNSGKFDGMTSEDAL
                     VKITEVLAKEGRGELKINYRLRDWLVSRQRYWGTPIPIIHCEGCGVVPVPEEDLPVML
                     PYDVEFTPDGESPLKKSKEFMNTVCPKCGKPAQRDPDTLDTFVCSSWYQFRYPDNKNS
                     KEPFNKERINKLLPVDIYVGGAEHAAMHLLYARFITKALRDMGCLNFDEPFLSLVHQG
                     VILGADGEKMSKSRGNVVSPDKYINESGSDVFRTYLCFGFNYIEGGPWSDDGIKAIIR
                     FLGRAERMVERVYELRGQKSRNEVAEKEKELNYVRHMAIKGVTKDSEIFQFNTSIAKI
                     MELLNALYKYDQEVEYKNIPYFEEVLADLLKLLAPFAPHFTEEMWEKLGYEYSVFNQK
                     WPVWDESALVRDSVELAIQINGKVKGRIEVPSAASEKEIEDMAMANPETKGLIAGKQV
                     LRVIVVKGRLVNIVIK"
     misc_binding    complement(29191..29437)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(29613..30566)
                     /locus_tag="PKU88_04915"
     CDS             complement(29613..30566)
                     /locus_tag="PKU88_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422646.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPX68659.1"
                     /translation="MSKTWSGKRYHTLNHFLREKFGEKVFKISLDAGFTCPNRDGRVS
                     SGGCIFCSPRGSGDFAGTSQNIVEQFHEVRGMMNKKWKSGRYIAYFQAYTNTYAEADV
                     LREKYYSVLNLEDVAGIAIATRPDCLPADVLDLLAEISSRTYLWVELGLQTMHERTAR
                     LINRGYGLDTYIASVKELKKRNIEVVTHQILGLPGENKRDMIQTVDFISNTGTQGIKL
                     HLLHLLKDTRLAEMYRQGEFQLMSMEDYIEVVVDCIERIPENMVIHRITGDGPRDALI
                     APKWSLKKFEVINAIEHLMMDRDTWQGKKYEPNNDIMNIFV"
     gene            complement(30608..32065)
                     /locus_tag="PKU88_04920"
     CDS             complement(30608..32065)
                     /locus_tag="PKU88_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPX68660.1"
                     /translation="MKNRWISFAAVTALIIIVLHLLFMYRIGQTGIPFNSTYRLQQIQ
                     RNIFWVIAISYTLVLLFAAAVIISINMDRRRYAESSLADNLKILEATVNATGDGILVV
                     DINRKILKANDLFFKIWNIPPDMCGLSDAREFLSFIRKQLKDSDVYEAWENSLYETCA
                     IEHYIASLHDGRIIDVFSNPLMDKGSLVGRVWSFRDITKQVNVEHELKKSYEEVKLIT
                     EALEYDKVKTEFFANISHEVKTPLNIIIGTLQLIELGLSNDNITADSDKMAKYTAIMR
                     QNCYRLLRTLNNLIYITEIDSGYIEMRLHNTDLAAIVKNITASVEKFLQSKEIQLELN
                     IEPDSLKIACDTDKIERILLNLISNSVKFSENGGKINISLYEKDKWAYISIKDNGIGI
                     PEEMKDLIFQRFRQVDKSFTRKREGSGIGLSLVKSLVEMHEGTIEVKSEYGKGSEFIV
                     KLPVRLAEDDEIAAAAEETSCVFVDRVNIEFSDIY"
     gene            complement(32333..33133)
                     /locus_tag="PKU88_04925"
     CDS             complement(32333..33133)
                     /locus_tag="PKU88_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPX68661.1"
                     /translation="MRRSKLLIFFLVITLSLTSVTFADTLKYGSRGAEVTKLQEQLKK
                     LGYFNGKATGYFGSMTKTAVRKFQSSKGIAVDGIVGKQTSKFLFESGTSSRSAASRTA
                     AASSKNAKIEMLDWWKEANKVFSIGTTAKVTDVGTGKSFKIVRTYGGNHADCEAATSE
                     DSKIIKSIWGGNWSWARRPIIIELNGRRLAASMAAMPHAGVDSQPANKILNKRSQGYG
                     RGYNLDKIKNNGMDGVFDVHFLNSRTHGTNRVDANHQKAVRKAASSEV"
     gene            33304..34635
                     /locus_tag="PKU88_04930"
     CDS             33304..34635
                     /locus_tag="PKU88_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPX68662.1"
                     /translation="MLVKNNANYRDLIVGADTGIPLSNGQIAASINFDNAATTPPFAS
                     VIQAIEDFAPWYSSIHRGTGFKSKYSSEIYENSRETIKKFVKADPGSLVVFVKNTSEA
                     INKLSYRLYHKNRRSVILSTCMEHHSNDLPWRNKYLVDYINVDNNGRLSMRDLERKLH
                     KYRKNLALVTVTGASNVTGYKNPIHDIAELVHKYDSKLMVDAAQLIGHSCVDMKPDGS
                     SRHIDYLAFSAHKMYAPFGTGVLIGHQSDFEKGEPEYRGGGTINIVTHDLIKWAEPPH
                     KEEAGTPNIMGVVALAEAVRTLASVGMENLEAYESSLLEYALDRISIIPDIKLYCDDD
                     PRDRVGIIPFNIAGLHHQIVADALSAESGIAVRNGCFCSQPYVQRLMKIPRNDIRKRI
                     KNPSLPHPGMVRLSFGLYNNYDEIDVLVNKLYEISANKELYKERYGQHLTH"
     gene            34799..35275
                     /locus_tag="PKU88_04935"
     CDS             34799..35275
                     /locus_tag="PKU88_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPX68663.1"
                     /translation="MNKRMNILMFSGEYDKAMAALILANTAREIQVEVTMFFAFWGLF
                     LVRDPEKFSAKDKTAYEKIFGAVTPKGPEQMPLSRMNMAGLGKKMLLEMMDDDSAPHL
                     IDFLKGARKKDVKFYGCKLSMEIMGLKQEEMISELEIMEAKDYLKDALESDLQLFI"
     gene            complement(35379..36497)
                     /locus_tag="PKU88_04940"
     CDS             complement(35379..36497)
                     /locus_tag="PKU88_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HPX68664.1"
                     /translation="MRDIMADKLTKAVIGLMLLVMLLFVCLEKPAGARKVFSGLSETE
                     DMTDSIHQIFRNRNNAIINGDLKLIQSLYDTSTKYGTWAHQHELTKVKYLHKWEKKQG
                     VKFTEIIPTVKVRSIKPKGEKYTINLMCSTEYKYVYENEPGVVNSFRIGTYHVLDLLY
                     KDEVWIITREWYKDPFEDSLNLGRIDVDSIHQYISTHAARDFSNIGDRRKNAVAYADK
                     YCGAASEEKYGYKYNKKYRNYNSQGGDCANFASQILHEGGKFRKTHSWNYNNKGATRA
                     WVNADGFKSYMLYSGRASLIAYGNYEKVYKASYKLLPGDFIAYEKKGDITHISVVTGA
                     DSKGYALVTCHNTDRNRVPWDLGWSNGKIKFWLIRVHF"
     gene            complement(36644..37117)
                     /locus_tag="PKU88_04945"
     CDS             complement(36644..37117)
                     /locus_tag="PKU88_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPX68665.1"
                     /translation="MKGKLNLLTKAAVLLAIAIVFQLVKMGQYVTGTGINAVLITAVG
                     VCGLPWAAAIGIMTPMLAVLLGVQPPPTIVLVPFIMAGNTVYVVLFRLLRRYNDYIGI
                     IAAALTKYILLYTAANIIVDKLPAPIRLAFSFPQLITAAAGGAIALIVLKIVQKK"
     gene            complement(37123..38862)
                     /locus_tag="PKU88_04950"
     CDS             complement(37123..38862)
                     /locus_tag="PKU88_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPX68666.1"
                     /translation="METVNITIDGIKTQVPKEFTVLEAAKSLDINIPTLCHLKGINEV
                     GACRICVVEVKGARTLQASCVLPVSEGMEVKTCSPAVMEARKTILDLILSRHERECTT
                     CSRNLNCELQKLCDEMGIDKVSYEGEKIVYAVDQASPSIVRDQNKCVLCGRCIAICRD
                     IQHTEAINFANRGIRTTVTAPNGKALTEVNCINCGQCIKICPVGALREKDDTHKVWSA
                     LGNPDLHVVVQTAPAVRVGLGEEFGMPVGTNVEGKMITALKRLGFDKVFDTDFSADLT
                     ILEEGTELLNRIKNGGKLPLITSCSPGWIKFCEHNYHEFLDNLSTCKSPQQMMGAVAK
                     SYYAEKAGIDPKNIFVVSVMPCTAKKFEANREEMALDGLKDVDVVLTTRELAKMIKQV
                     RINFTALSEGKYDSILGDSTGAAVIFGATGGVMEAALRTVADILTGQDLKKIDYTPVR
                     GMEGIKEASVNVDGVDIKVAVAHGTGNAAKLLDSIKDGKAEYHFIEVMACPGGCINGG
                     GQPILLDKEKTEEVKKLRAQGLYKIDKEDRRFRKSHENPEVKKLYEDYLEEPNSHISH
                     RLLHTKYIERDRA"
     regulatory      complement(38999..39118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(39229..39819)
                     /locus_tag="PKU88_04955"
     CDS             complement(39229..39819)
                     /locus_tag="PKU88_04955"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025434778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPX68667.1"
                     /translation="MSLVNTAVILAGGKSTRMGFDKQLIRVKDICIVEHLVEMLSPVF
                     KNIIVVTNKPELYKDKNIAIAEDVYKGYGPIGGIHAGLSKSQSMYNYFIACDMPNINK
                     CYIEYMIKRLEEDSFKSDAVITKKADWIEPFNAFYSKKLIPAIEDNIKKGKVRTMELL
                     EISNVLYIDEAIARGFSPDWSMFANLNTREDLKGLV"
     gene            complement(39946..42642)
                     /gene="fdhF"
                     /locus_tag="PKU88_04960"
     CDS             complement(39946..42642)
                     /gene="fdhF"
                     /locus_tag="PKU88_04960"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(41593..41595),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPX68668.1"
                     /translation="MVTFTLNGKVVTAKEGTTILDAAKENGVDIPHLCYDKNLSAFGG
                     CRLCVVEVQGAKALVASCTNTVSEGMVIQTESETVVNVRKDLLDLLLASHPEDCLTCE
                     QAGNCKLQDYAYRYGIKRSSYKGEKKSYDIESLNPVMERDQSKCILCGKCVRVCREVQ
                     VTGTIDFINRGFETKIATAYDVPISTENCRLCGQCISVCPTGAIINKQLKGTRPWEVS
                     KVRTTCPFCGVGCNFDLNIKDGKVVGVTPNPDSPVNGTSICVKGRYHIDMINSPERLT
                     KPLAKKNGEFVEISWDEALHTVTEKLKETKEKYGPDAIAGLSSARCTNEENFLFQKMM
                     RVAVGTNSVDHCARTUHAPTVAGLAATLGSGAMTNAISEVEGNEVLFIIGSNATEAHP
                     IIGNKMKRAARNGSKLIVIDPRRTELAEYATLWLPLNSGTDVALINGLMHIIVKEGWH
                     DKEFIETRCEGFDELLKAIEKYTPEYVSKITGISEELLYETAKLYTSTKKAGIFYTLG
                     ITEHITGTDNVMSLANLAMLVGHLGIENAGINPIRGQNNVQGACDMGALPNVFSGYQS
                     VTDEKAIQFFEDFWKFKLNRNTGLKIPEMFDGSHEGKVKAMYIMGEDPVLTDPDANHV
                     RKALEKLDFLVVQDIFLSETAKLADIVLPAACYAEKEGTFTNTERRVQMVRKAVEAPG
                     EARPDWEIICDVAKGLGAGGFDYKDAEEIFKEIAATTPSYRGMTFARLDKNGLQWPCP
                     TEDHPGTQYLHKGTFPKGKALMKAIEYKVPAEAVDEEYPILLSTGRMLYHYNITSRFS
                     EKLDAIKPYELAEINPADAEKLGLKEEDFVRVTSRRGSIVTRVTITNKVKPGMMSMTF
                     HYRESPANELTNSAFDPVTKTGEYKVSAVKVEKVKDMQGTAV"
     gene            43029..43364
                     /locus_tag="PKU88_04965"
     CDS             43029..43364
                     /locus_tag="PKU88_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPX68669.1"
                     /translation="MYNIKYKLWLDKNGVIFGTGSYLLLKGVKEKGSLAGAAKAMNMS
                     YNKAHNLIKKIEIRLGFRLLYSKTGGAGGGISLLTIEAEELVSAYELFYNECEEAINT
                     IFKKYFPDH"
     gene            43432..44454
                     /locus_tag="PKU88_04970"
     CDS             43432..44454
                     /locus_tag="PKU88_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HPX68670.1"
                     /translation="MNTVKTENAVGMVLGHDITEIIPGEYKGAAFKKGHIITENDIER
                     LLRIGKEHVYVFELGEDELHENDAALRLGKLFAGPGISFSEPSEGKTSLKSMYRGLLK
                     INRDLLEQVNDTEDICLATIQENICVEKDALLGGCRIIPLSIKKEKIEHVENMVKGKD
                     PMFKIKSFINLRTALIVTGSEVYKGRIKDEFGPVVEKKVKHYGSGIFNKTIVTDELDE
                     IKNAVLDAKKAGAELIIVTGGMSVDPDDKTPGAIKATGADIITYGTSVLPGAMLLFAY
                     LDGTPVFGLPGCVMFAHTTAFDLLLPRVFAGEKIIRKDITRMGYGGQCLSCEVCKFPY
                     CNFGKH"
     gene            44464..45699
                     /locus_tag="PKU88_04975"
     CDS             44464..45699
                     /locus_tag="PKU88_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPX68671.1"
                     /translation="MLENITVETAKRILSEEKNDIKQSELYILDSLDHVLAEDIVSET
                     DMPPFDRSPLDGYAYKSEDTVNAAENSPVTLEVIDYVQAGYVSRKKICNGQAIRIMTG
                     AKIPEGADVVIQYEDTEFTDKTVKIFRYLRPQSNIVKAGEDMRAGDLILRKGITIGPA
                     EIGVLASNGKTHVKVYSKPTIAILSTGDELLEIDEELADGKIRNSNSYVIAAQVKKYG
                     GNAKILGICMDRVNDVKKKLDSALKWADMIITTGGASVGDADIIKEAFKAAGADILFW
                     KTRMKPGTPIVAAKHGNKLLFGLSGNPAAAFITFEIFVGPAILRLMGKPKHKYMKVES
                     ILESDFNKVSEQDRYLRAFTYKKEGAYYTVLPDKHSSGVLSSLSGKNSLLLIPGGSSP
                     YKKGDRVTVELINHLEAEL"
     gene            45696..46184
                     /gene="mobB"
                     /locus_tag="PKU88_04980"
     CDS             45696..46184
                     /gene="mobB"
                     /locus_tag="PKU88_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HPX68672.1"
                     /translation="MIPVFSVVANNSKVGKTTVLCDIIKELKSRGYRICTIKHDVHGF
                     DIDHPGKDTWKHAQAGSDFVVISSPKKLAMIEKVEKEYSLDEVISKISNVDIIITEGF
                     KQERKPKLEVYRKEFPNEIWSKDEDLFAIITNTPLNKGVPQFDFSEIKEAVDLIESLF
                     LK"
     gene            complement(46190..46687)
                     /locus_tag="PKU88_04985"
     CDS             complement(46190..46687)
                     /locus_tag="PKU88_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HPX68673.1"
                     /translation="MEGFSVGIITVSDKGSRGERADASGPAIEEIMKQIGGRIEKYVI
                     VPDEMRDIKEAIIDMSDTLGLNLILTTGGTGFSKRDITPEATLEVIERYVPGIPEAMR
                     AKSLQATPKAMLSRAQAGIRKQSLIINLPGSPKGVRENLEAIIPALKHGIEILTGQAS
                     ECGGK"
     gene            complement(46706..47251)
                     /gene="moaC"
                     /locus_tag="PKU88_04990"
     CDS             complement(46706..47251)
                     /gene="moaC"
                     /locus_tag="PKU88_04990"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPX68674.1"
                     /translation="MNSKNKKEVQGVGELTHINAKGYAKMVDVSEKEESVREAIARGS
                     VYMKRETLKAIAEGRIRKGDVLSVAQVGGIMGAKSTPDIIPMCHNVSIYGADIEFQAD
                     YENSKIDIEAVVKTVGVTGVEMEALTAVSVAALTIYDMCKAIDKLMVISDIHLVHKRG
                     GKSGEFNFSGKLNEDINNTKC"
     gene            47472..49838
                     /locus_tag="PKU88_04995"
     CDS             47472..49838
                     /locus_tag="PKU88_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPX68675.1"
                     /translation="MFKISSKSIMFKVLVMILTICMLESFLFTIMSERLTGSILENMT
                     IENSRKNAELYSEFIGSWLGERMREINMYANSPVIRAMDWNNIELYLKQEVKEKLDVY
                     DHFMVAESNGDYNTTLRRNVGNVSDREYFKHVMEGKTVVSNPIVSRSTGKPEAIIASP
                     ILDNNGKIKGVMAGAVNLMKLSRIIDNLKYNYPNSYSYIVDKEGQIIAHPREELILRH
                     NITKESDVISRDIAEASAEILTNDRGSNTYSFEGIVSMNYYHIIPNTDGWKLIIKIPV
                     NYWQTPMRYTSSRLILIGLAGLIIASIVGFFIAKSISTPIIKLKEVFTRATAGDLTVR
                     SEISTDDEIGDAARSFNKMMDTISKLTYYDSLTLLPNKLHFDAILCREIKKSASNNTM
                     LALIIFDIDRFESINNALGHTVGDKLLKSLAEKVSCLEDNNHVVSRIGEDMFAVLLKN
                     FKEKEDVIKLSDEIRYIIRQPWVIDEYRFYITACVGIAIYPKDGESGDSLFKNAFSAM
                     QKAKVHGRDNFNFYEPSINELLVEQMNLDSSMHHALDNGEFSIYYQPQMDTRTQEIIG
                     CEALLRWNHPELGMIGPNEFIPISEANGLITLIGRWVLYTACMQNKLWQDSGFKPIFV
                     SVNLSPAQLIQENFIDMVSEILEETKLLPEYLELEITESVAVKNYDYITGILEKLKKM
                     GIRIALDDFGTGYSSLNYLKNFAITTLKIDRSFIHDINENPKNSAIVSSILAMGRNLK
                     LCITAEGIETKEQYETLRDRGCDIMQGFYFSKPLPLHEFEKLMKKPGD"
BASE COUNT        12971 a        12310 c         9090 g        15544 t
ORIGIN      
        1 gtaagcggat aagtatggtg cctggcgcct aattcaactt ttcctctatg agcttagcca
       61 gggcctcagc atcttccttt atttccgtct cgttagctcc ctccagcata accctaatca
      121 agggctcagt gccggaaggc cttataagca ctcttcccct gcccttgagc tttttctcca
      181 gtcctgttat cattcctgct atagcttcat ccttcatata cttatccttg ttctcattct
      241 tcaccttcgc gtttaccagc acctggggca gttctttcat aaaggaagca aggtcggaca
      301 gcttcttgcc ggtttccttc acaactgcca gcagctgcag ggctgtcaaa agcccgtctc
      361 cggtagtatt atgatccagg aatattatat gtccggactg ctcaccgcca atggagtaac
      421 ccattgaaac catttcctca agtacatacc tgtctcctac cttagtcttt acaagattgc
      481 acttctcccg cttcaaagct atatcaaggc ccatgttgct catgacagta gcgactaccg
      541 tgtctttctt cagcatgccc tttcctttta aatgcctggc aaatatggca agcatgtgat
      601 ctccgttaac aatatttcct ctgttgtcta ctgcaataag cctgtcagca tccccgtcaa
      661 aggccagccc tatatgggct ccggattcca caaccagctt ctgcaggcct tcagggtgtg
      721 ttgagccgca ttttacgttt atgttctttc cgttgggctt gttgtttatg acagctatct
      781 cggcaccaag gtccgcaagt accgaaggcg ccgctatata tgatgcgccg tttgcgcaat
      841 caaccgctat cttcagccct ctcaggttga catctatggt ctttttgaga aaatcaacat
      901 atccgtgtac cgcgttatct acctcaacct tcttgcctat atccgcccct gtgggatatg
      961 ctatgccgtt ttccggattc agcatcaggc tttctatcct gtcctccaat tgatccgaca
     1021 gcttatatcc ttcaccgttg aaaaacttta ttccgttata ttcaaaggga ttgtgcgaag
     1081 ccgaaatcac gactcccgca tccaggccca tatgcctcgt aaggtatgcc actgcaggcg
     1141 taggcaaaac tcccagggac accgcttctg ctcccatcga gcatatacct gcaatgaggg
     1201 cagcttcaag catgtcaccg gagattctgg tatccttccc tacagcaatc cttggttttt
     1261 tgtgggtttc ttccgttaat acatatgcac caattcttcc aagattgaac gctaaagcag
     1321 ggctcaactc tttgttggca atccctctta ctccgtctgt tccaaacatg cgtcccatta
     1381 tatgacccct ttctcaacag tgaaggtcat tttcactgat gatatgattt ttttggatat
     1441 agtaatatta tcataactca tatgttttgt tcaaatcatt acatgttatc gggtgtttct
     1501 atccccgcaa ggctcaggcc tgtcttgata actattccga ccgctttggt aagctgcagt
     1561 cttgcattct tgacattttc gtcagcattc agtatgggat gggtgttgta aaatttgtta
     1621 taggccttgg ctatctccgt tagatggcgg acaactacaa agggctcata cctttccact
     1681 gcttccttta ccgaatcctt gaaggctccc agaagcttga ccaactggaa ttcatcctct
     1741 gtggaaagaa aagtcaggtc cgccgtatcc ggtacgtatc ccgccttctg caatatgctc
     1801 ctgcctctta cataggtata ctgaacatag gggccggaat ctccgtcaaa gttcaatgca
     1861 tcttcccagg taaacacaac atccttttcc ctgttattct taaggaaggt ataaagaagc
     1921 gctcccatgc cgaccttcct tgcaacctcc tccttgttct ccaggttcgg attcttatgc
     1981 tctattactt ccatagtctt atgtacagct tcattcatta catcctttgc aaaaacaacg
     2041 tcgcccttcc gggtcgaaag ctttttgtct gcaaacttta caaggccgtg gcctatgtga
     2101 acgcagtcct cggaccactc aaagcccata agccccaggg tgctgaaaat ctgattgaaa
     2161 tgcagggact gtgttcctcc caccacatag atgttcttat agaaatcgta ggtcctcttc
     2221 ctgtatatgg ctgctgccag atccctggtg gcataaattg tggcaccgtc agacttaagt
     2281 atcattatcg gaggtatgct gaactgctcc agatcaacta tttttgctcc gttgctgtcc
     2341 ttaagcagtt tcttttcacg aagtatttgg acaacctcat ccatcttgtc actgtaaaag
     2401 ctttcccctg catatgaatc gaactccaca ttcagcatct cataaatctc gctgaattcc
     2461 ctgaggctca gatccttgaa cctttgccaa agtatggtta cttcggaatc tccttcttca
     2521 agctttttga agtatgctct ggcttcatct tcaagggctg ggtccttttc agcctcttca
     2581 tggaatttga catatatccg gaaaagctcg cttatgggtt ccttctcaag ggcttcttca
     2641 tttatccatc tcttatgtgc ggatataagc tttccaaact gggttcccca gtctccaaga
     2701 tggttgattc ttatcgtatt gtagcctgag tgcctgaaaa ccttttcaag ggcgctgcca
     2761 agtattgtat tgaacaggtg gcctacatgg aagggctttg ctacatttac ggaagagaat
     2821 tcaactatta cattcttatc cttgccttcc tccgagcttc cgaatttctc tcccttctca
     2881 agaatctcct gtatcataat ccttatgtat tccttcttat taatgaaaaa attcacatat
     2941 ccccctactg cttccgcctt ttcaactatg ctgtcagcct gcaccttccc cgcaatctcg
     3001 gcagcaatca tattcggtga ttttctcatc actcttgcca gctgaaaaca aggaaaagca
     3061 taatccccca tattagtctg gggcggaatc tctataagct ccatgacctt atcaaattcc
     3121 atatcaacta acgtatttat tttttttgcg atttccactt tgaaattcat atttatccct
     3181 ccaataagtt ttattatata taaaatgcag aaaaaaatcc atcccgaaag gaggatggaa
     3241 gatggtggtt ttttattttc gggatggatt taattaaatt attttatgca agctgcccct
     3301 cttaaaagtg cttctttatt cagcggtata acgctggctt tcttacctgc gaaaatatgt
     3361 gtgataatat tcattattga atcgaaggat gctgcacctg atgactggag atatgctccc
     3421 agcattacta tgtttgcaac cttgtctgtt cccaactcgt ttgctatttc gtttgccggc
     3481 acgtagtaaa cctttatatc ttctcttgtg gctttcttgt ctattaaaga gctgtttatc
     3541 aaaagtgctc cgcctggtac aacagtattt tcatacttgt ccagtgacgg tctgttcatt
     3601 actatagctg ctgtggcttc cgtaactatc ggagcaccga taggctcatc agaaactatt
     3661 acgctgcaat ttgccgtgcc tccacgcatt tcggggccgt atgagggtag ccatgacaca
     3721 ttctttcctt cttccatggc tgcctcaaca agtattttac ccatagccat gacgccttgt
     3781 ccgccaaagc ctgccattat aatttcatgt gccattattt cacctcctca atatttttga
     3841 agtttccaag gggataatat ggtatcatat tctcctggag ccacttcaat gcttctatag
     3901 gagttttacc ccagtttgtc gggcaggtag acagtacttc aacaagagta aaacctttgc
     3961 cttccatctg aagctggaaa gctttcttta tagctttctt tgcctgaata atatgcttta
     4021 cgtcatgtac cgatactctt tctacaaatg cagccttagg aatagttgca agcatttcgc
     4081 ttactcttaa aggcatacct gcatggttct cgtcccttcc gtaaggggct gtggttgcct
     4141 tctggcctac aagagttgta ggagccatct ggccgcctgt cattccatat atggcattgt
     4201 ttacaaatat tgtggttatc ttttcgcctc tcattgccgc atggacaacc tcggcagcac
     4261 ctattgatgc cagatccccg tctccctggt aagtaaacac tgcagcattt gggtttactc
     4321 tctttatgcc tgttgcagca gccggagctc ttccatgagc tgcttcctgc atatcgcagt
     4381 taaaataatc atatgcaaat acggcacatc ctacaggagc aacaccaatg gcatcatcta
     4441 taagattgag ctcatcaagt gattcagcca ccaacctgtg tattatccca tgagtgcagc
     4501 cgggacaata atggaagggc ttttctgcaa gtcctctggt cttctcaaat acaactgcca
     4561 ttactttgca ccccctaata tcttcttagc ttcttcagca atagccttag gagttgggaa
     4621 cataccgccc gttcttccat agaagtaaac aggtttcttt ccgtttacag acagcttcac
     4681 atcttcaacc atctgtccca tactcatttc tactgacatg aaagctttta cgccggcctg
     4741 ttctgctgct ttctcaaagg ctttggccgg gaatggccaa agagttatcg gtcttataag
     4801 tcctgctttt attccttctt tagcaaggga gtttattgca ttcttaacta ttcttgctgt
     4861 tgttccataa gcagcaaata ctatttctgc accttcaagg ttatactctt caactttgat
     4921 ttcattcttt tcgatttccg catatttttc aaacaaatgg aagttgtgtc tttcacaatc
     4981 tgccggatct ataaacaagg aatttatgac attagtcttc ctctttccct tggtacccac
     5041 tgtagcccaa gtcttttcag gcagttttct cttttttact tcagtaaatt ccaccggctc
     5101 catcatctgt cctatcatac cgtccccaag aatcaatact gggttcctat actggtctgc
     5161 tatgtcaaag gcttcctgag tcaagtcaac ggcctcttgt actgaagcag gtgcaagcac
     5221 taccaacctg taatcgccgt gtccgccgcc cttgcaagac tggaaatagt ctgattgtgc
     5281 aggctgtatt ccgcccagtc ctgggccccc tcttaccata tttactatta cacaaggcag
     5341 ttcagctcct gctatatatg ttataccctc ctgtttcaag cttattcccg ggctggatga
     5401 ggatgtcata acccttgcac ctgcaccagc tgctccataa accatattaa tagcagctac
     5461 ctcagattct gcctgaagga aacatccgtc aatctgaggc attttcatcg ccatgtactc
     5521 aggtatctca ttctgcggtg taatcggata gccgaagaaa aatctgcagc cggctttaat
     5581 cgccgcttca gctacggcct cgttgccctt ccataattcc ttagccatta ggcttctacc
     5641 tcccttttta tctttctact tttattacaa cgtcaggaca cattcttgca caagaagcac
     5701 atcctgtaca tttgtccatt tccttaactg tcgccggatg ataacccttg atattaatcc
     5761 tatccttatc cataacaaca attttgacag gacatactgt ggtgcagagc tcacaaccct
     5821 tgcatctgtt ttcgtcgaaa gttaccttag ccattactta acctccttac taatttgttt
     5881 tgttagttac aatgctgata taattagtcc tcccaaggct ttttcatata tatatttatt
     5941 ggcaatacat cacctcggag tcctttcggg agcacgtcaa caaacttctt ttcaacaacc
     6001 acatacttga ttggcagccc cagaaccttg gatacatctt cacaaagctt ctgccccctc
     6061 attatatcct ctacagtggt attgccgcag agatgagtat tgttaaccaa tgcagtaacc
     6121 ttttgcatag agccgtgttc aatggatctt aagtagctta ttgccccatc cactgtactg
     6181 gtctgatgtc tattggcatt cagcacaaaa aacatatcat aaggctccct gtcaaagtac
     6241 tcgtggtatc ttcccagagc cctggctccg accttgtctc cgccgacatc caatacaacc
     6301 tcataagagg tatcctgcaa aacggagtat atttccggtg aaagggcggg caggtctccg
     6361 aatatgcctt ttactgaaga agatataacc cttatgccct tttctaccag cagcggttct
     6421 ctttcccttg accggaagta tggatttact atgtccaagt caactattgc tgtcttcttc
     6481 cccgcttccg caagagcaat tgcataattc acagcaaact cggtttttcc gcttccgtag
     6541 tggcctgtga ttatccttac tcttctatcg ttatacattt aatcatccct atcaggctgt
     6601 gcagcctatt tatactccct tgcagcttct tccccacgaa gcaccctgag cccgccctcg
     6661 gcaagagcta tcatttcatc ctcgccggga tatatcttta cctctcctat gaactctacc
     6721 ctttccttta tccagccggt aagaagctta tcataggcaa ggccccctgt caggcagacg
     6781 gcatccacct ttccttcaag cactgcggcg caggcaccta tctcctttgc cacctgatat
     6841 gccattgccc tgtacacaag ctctgctttc ttgctgccct cccctatcat tcttacaact
     6901 tctctgccgt cattggttcc aaggtatgca acaagtccgc cttttcctgt gattttcttc
     6961 tttatttcat ccaatgtata tttacctgag taacagagtt ttatgagccc gctgaccggt
     7021 aatcctccgg ctctttccgg agagaatggg ccctcaccgt caagagcatt gtttacatcc
     7081 accactctgc cttttctgtg tgcgccgact gatattccgc ctcccaaatg ggcaattatc
     7141 agattcaaat cctcatactt tctgttaaga tccgcagctg cccttctgcc tacggctttc
     7201 tggttaagtg catgccatat gcttattctt tctaaatcag gcattcctga gattctcgca
     7261 atttcatcca tttcgtccac cacaaccggg tctacgataa atgcctgtat cccaagctgg
     7321 gaagctattt catgggcaat tatccctcct aagtttgaag catgttgtcc catgactgca
     7381 cccctaagat cttcaatcat ttttccattt acatggtagg ttccgcctac catgggcttt
     7441 aagagtccgc cccttcctac aactgcattt agcttggtaa tatttatccc attggcattc
     7501 aatgtatcaa gtattacatt ctttctgaat tcatattgct caataattgc tccaaagggg
     7561 gcaagctcct cgttggaatg tcggagagtc tcctccagaa caggcttctc attatcaaaa
     7621 atagcaatct tcgttgaggt ggaacccgga ttaatgatca gcaacctgta ttcctgcatt
     7681 ttaaaacaac ctcctaaatt tgcggaaccc cttcaatgga tccgctgctc gtatctttta
     7741 tctactttcc tgccataagt actgctatgg caatggaatt cagcttggct tcgtccgaat
     7801 cggcacggga agtcattacc acaggagctt ttgcccctac caatattccg gcaacctttg
     7861 atcccgcata atataccatt gatttgtaca aaacatttcc cacttctata taaggaacca
     7921 gcaggacatc cgccagtcct gcaacgggat gggttacccc tttatgcctg gctgcctcta
     7981 cggatattgc attatcgagt gcaaaggggc ctccgacaac gcagcctttc agttctcccg
     8041 attcattcat ttttaccagt tctgccgcat caagggtagc ctgcattttg ggatttacct
     8101 tctccacagc ggccagcaca gcaacctttg gattgttatt acccagggcg ttggccacct
     8161 gtacgacatt ttcaattatc tgcttcttct gaatcaaact gggagccaat atcattgccg
     8221 gatctgtaac atagaaaagc ctgtcatatc ccggcacttc aaacactgca acatgagaaa
     8281 gaacattgcc cgtcctgagg cctatgtttt catccaaaac cgctttcagc acaatagccg
     8341 tatcaactat acccttcatg acaatgtgtg ccctgccgcc ggaaaccatt tcaaccgctt
     8401 tcctgctggc ctcattctta tcctttatgt caataacttc atatttgccg atatcaatgc
     8461 cgcattcctc agaggcttta atgatttctt ccctgtcgcc tacaagtatg gcttccgcaa
     8521 taccaagctt ttttgctgca tttaccgcac ttaatacctc cggatcctga gcaactgcaa
     8581 cagcaatggt tttgggccct tttgcctttg cagactcaat agtttcctta aaagtcctaa
     8641 tcatttattt cacctcttcc tcatatatct ttgctttctc cgcacccttg agtactcgta
     8701 aagcaccttc agccaatgcc tgcatttcat cctctcccgg atatatcagg acaggcccga
     8761 taaattccac tcttttggtt atttccctgc acaacagatc cgaatatgca aggccgccgg
     8821 taagtattat tgcatcgagc ttcccgttaa ttaccgtggc catagcacct atctccttgg
     8881 caatctgata tgccatggcg tcaaaaatga gggctgcctt ccggtctccg gcctcaatca
     8941 ttttgactac atcccttgca tcattagtcc ccaaatatcc gacgagtccc gcgctgccca
     9001 cagccttctt tttcatctcc gcctgtgtga attttcctga aaaacacatt tttactatat
     9061 tgcctacagg aagccctcct gccctttccg gggagaaggg gcccgcatcg ttagcaccgt
     9121 ttacgtcaac tatcttccct ttcctgacgg gagaaacgga tatccctccg ccaagatgtg
     9181 caataacaaa atcaacgtcg gtaaattcct ttcccatatc ctttgcagct cttctgccga
     9241 cggcttttat attcaaagca tgtaccaaag ccggcctctc aagttcaggc attcccgaaa
     9301 tacgcgcaat atcgtccatt tcatcagtag ctaccggatc gacaataaaa gcgggaatac
     9361 cttccgtatc cgctataccc ctggcaatta tcgccccaag gtttgatgca tgttcaccct
     9421 gaacagcact tctcaaatca tcaataattg catcggtaac gatataagtg ccgccgggca
     9481 tcggtttcag caaaccgcct cttccaacca ccgctgccag gctgcccgtg ctgatgtccg
     9541 attctttcag ccaatcaagt atcatttttt gtctgtactc atactggtcg gtaattttct
     9601 catacttggt cacttcttca gcggagtggc tgagattttt ctggagtata ttctcttctc
     9661 ctttgaaaac tgctgccttt gttgaagtgg atccgggatt tattgccaat atgtattctc
     9721 ttgacacaga ttcaccgctc cttacatata ttattgctta ttattctttt cagattatat
     9781 ttatgcaaat agtatgccac aaaagcagtc gataaaaata aaaacctgtc gtttagttta
     9841 tgtcaaccaa gcgacaggtt aaaaaatgtt cataatgctt atactttttt ttgaaaaaat
     9901 tttcagacat tgaggctttc tttttgtttg ttacagaaaa aactcggcat ttgaaaaata
     9961 atgcatgcaa attcttgcaa attaataatc cgttttgcaa atatttgcat gcaaattact
    10021 cgagtccgta cctttcaagc ttataataca ggcttcttaa agatatgttc agattctttg
    10081 ccgcttttgt cttgttattg ccgcattctt tcataacctt tgctatatgc tctttctcgg
    10141 ctttctccac tacactgtcc aaagtcattg atatgctgcc ccggcctttc atacgggcgt
    10201 cgggattatc cccggcatcc aaaaatcctt cgagcttggg caagtggttt tcctgtataa
    10261 ccgtatcact tgccttcata ttgataattg cacggccgat aaagttatcc agttctctta
    10321 cgtttccggg ccagttgtac tccttcagcc tgctgacggc ctgctgcgtt atgtcgcgaa
    10381 cgttgcgccc gtattgttga ttaaacttct ttatgaagaa aattgataaa tcgtaaatat
    10441 cgcctttgcg aagccttaaa ggcggaatcg ttatgggcag cacattcagc ctgtaataaa
    10501 gatccgctct gaacctgccg ctttcaatgg ccacctccag atccacattt gttgctgcga
    10561 ttatccttac gtcaatactt atcgactttg tgcctcccac ccttacaatc tccttttcct
    10621 gcagcactct aaggagcttg gtctgggtgc tgtgaggtat ctcccctatc tcatcaagaa
    10681 atatagtgcc tccttcggct tcctcaaaaa ggcccttctt tccgcctttt ttcgctccgg
    10741 taaatgcccc ttcttcatac ccgaacagct ccgattccaa cagggtttcg ggaatagccg
    10801 agcagtttac ctttatgaat ttcctgaact tcctttcact ttcattatgt atggcatggg
    10861 caaatatctc tttgccggtg ccgctttccc ccctgagcaa aacggttatc ggaatctccg
    10921 cagccttctt tgctttgtct atggcagaca gaagcagcgg atcgcttcct attatatcct
    10981 caaaggtgta tttagcctcc aggcttctta ttatattctt ggcgtttttc aattcattgg
    11041 aaagctttgt tatctccgac atgtcatgaa taaccccgac actgcctctc agctctccgt
    11101 taacaattat aggggcagca ttcaccagta cttccttacg tgcaggcccc accttcatta
    11161 aagcaccctt aatgggcttt ctggtcttga gtacctgcat atgaatgctt tcgccctccg
    11221 caatatcaac agtagcaggc tttccaatga catccttttc agacagtccg gtaattttga
    11281 tataggccgg atttatcatt accccaagcc cattctggtc tacaaccgat atggcgtcct
    11341 gtgtggcatt aataattgcc tccagcaagc tgtacacttc attcaggttg gttatctcct
    11401 ccgcaagctt gataatctcc gtgatatccc taaataccgc aaccgccccg attatcctcc
    11461 cctttttgtc cttcaccggc atcctgttgg ttattatttt tatattcccc agatcctgct
    11521 ggcggttcag ctcagactca cctgtttcca atatatacgg aagccttgta tcttttatta
    11581 cgtcagccac atatttcccc attgcatcct ttgcttcgat tcccgtgagc ctttccgcag
    11641 ctttgttgaa aagtgttatt ataccgtctg tgtcaacacc aatcatagcg tcatgagtag
    11701 agttaagtat aagctctata tcttttttca tacaacaacc ttccttacat caggtccggg
    11761 gttcggggtt cgaggttcgg gtaattggcc ggttctttaa agcctgccct aatcctcgca
    11821 cctcgtgcct cgcacctcgc agcctgtttc tagtggcctg tgtcttgaat ttattttatc
    11881 ggcttaaagt tttgtattat tctctccgct gcctttattc cgtctactgc cgcacttaca
    11941 attcctcccg catagcctgc gccctctcct acagggtaga ggttttccac agattcgctc
    12001 tccatgttat ccttccttac aattcttaca ggtgcggagg ttcttgtctc aactcctgtc
    12061 attatcgcat tactgcttgc aaaacccttt atcttcctgt caaaatccct gagagcttcc
    12121 ttcattgaaa gagttacata ttcaggcagg cacaaatgca agtcacaagg tttaaccccc
    12181 ggctcatagc ttggcttgac cctgcctgct gcccgggaag cttttccttt aagaaaatct
    12241 tccgccagct gcaccggtgc atggaagttc ccgcctccca gcttgtatgc ctttccttcc
    12301 catattcttt gaaattccac tcccgataaa ggatggctgc ttttaaaatc atcgggagat
    12361 accgatacca ccagtgcgct gttggcattc tccctgtccc tcttgtactc gctcatacca
    12421 ttggtaacaa gcatccctcg ctccgacgcg gcagccacta cctgcccccc cgggcacatg
    12481 caaaagctgt atacagccct accggttgaa ctgctcctgt ccgtaagcag gtaatccgca
    12541 gctccgagcc tcggatgccc tgcaaagctg ccgtattgag cctcgtctat catactttgc
    12601 agatgttcta tcctcacacc tatcgagaag ggcttgggta ttatccgcac acctctttca
    12661 agaagcatgc cgtatgtgtc ccttgcgctg tgtcccaaag ccgctattat tatatttgat
    12721 tcaattgcgg atttcccatt tactattaca gctttagccc ggccttcttc caccaaaata
    12781 tcggtaacct tggaaagaaa ccttacttcc cctcccagtt tcgttatcct ttttctcata
    12841 ttttttatga ccgttttcag tatatcggtt cctatatggg gtttggcctg gtacagtatc
    12901 tctttgggag caccctcctc cacaaaggta ttcagtaccc tgcttattct tctatccttg
    12961 attctggtgg taagctttcc gtcggaaaaa gtgcctgccc ctccttcgcc gaactgtata
    13021 ttggattcct catccaatac accctgcctg aaaaatgtat ttacagcttc agccctttta
    13081 tccacatcgc tgcctctctc aagtatcagc gggctgtatc cgtgttgtgc caaaactaaa
    13141 ccggcaaaca atcctgccgg tccgctgcct attattattg gcctgttgtt tatcggcctg
    13201 ctgccataaa caagcttgtt ttcttcctca tattccgctc tctcattgac cctggcaaca
    13261 tcctttaccc tgaggtaccg ggaggggctt gtgctctcat ttatttcaac ctccgcagaa
    13321 tacaccagta atatatcctt cttgtccctt gcatctacag atcttctcaa tattctcaga
    13381 gaagatatat ccttttccct gatcctgagc ttttccgccg caaggaacct taccatatcc
    13441 gcatcattat caaagggaaa atccctgaca ggctgtttca agttatttac ctcaaaatgc
    13501 aattaatcat ctccctaggg ttcctgcagc ttagctttgc ttatcagtac aactgcggct
    13561 tggttcgggg atacctccag aacctctatt ccttgaggcg cctcccataa tacattcagg
    13621 acattgctgc cctcaacggc atccttcagg tcaacatagt atttgacagt tctcggcgcg
    13681 tcggcaatcc ggctttccgc ccctcttaag gttatcttta tatctccttg tatacttact
    13741 aactccaaat cttccggaag attaacatat tcaaggtctt taacaataat ctcgctggtt
    13801 gcaattttct ctatattcac cgataccttt acaatttcat cggcattaac gatttccaaa
    13861 ccttccggta ttaccagcct tacttccttt gatacagcgg cttttacttc agtaatatca
    13921 attttctcag tttttatgga agttatgcct tcaagaatct gctgctttcc ggttacaagc
    13981 acttcctcag gctgtaccga ggcaccgctt acaacgaatc ctgctgccgg ctgtcccgat
    14041 atatccatac tcaggcttac ccactttgta ttctctatag ggatgctgac ttccacatta
    14101 cctggttcta cggtaatatc ctgaatatcc tttccggttt tatccagcac tcttaccgga
    14161 agtacttttc ttacttcagc attggcgctg gcaatatcca catccaccct gacctcatga
    14221 atcatggcta ttttcgattc cgctcccgta attacaacat cgctgggagt caatgaatgc
    14281 atcataatcg ccataccgtg ggtcggatta cccatgatgt tcagtttaac gcttctttga
    14341 acgctgatct ttgattcaag cataatctta aggctgtcca tggatctcgt tattatgttt
    14401 acgccttcag gtatgccgtc gacatttatc ttaaggaagt tctccccctt caggttatgg
    14461 cctgccaaat ccgcataggc atctacagtg ttggcattca gcttatctaa aacatccttc
    14521 tttcccttta ttctcatggt cagtgcaaaa ttgtcagtat cgttgacaat taccatattg
    14581 cttttctcaa ggaatacagt gtttatgagc cttatgggga ctgtaacatc ctttgttatc
    14641 tcagggttct gctcggttat cacatagaac cacaaaagca gtgccagcat gaccgagagt
    14701 atccttattg tatgatcctt attaatcagc ttattattca ttcttaacac cccatttcat
    14761 ccatatgggc tgtttgtttt ccctggcctt gatgctgttt ttgattattt ccctgaggga
    14821 ttttgtatcc aaataccttg aaagcctgcc catcttggca aaggatatca ctcccgtctc
    14881 ctcagatact attattacca ttgaatctga aatttcagtt atacctattg ccgccctatg
    14941 ccttgttccc agctccttgc tcagattctg gctctgggta agaggcagaa agcagcctgc
    15001 cgcagcaatc ctgtttcttc tgattatcac agcaccgtca tgaagagggg tattggggac
    15061 aaacaggttt atcaatatct cattgttcac tacggaatcc agcttggtgc cggtctcaat
    15121 gtattcgttc aaaccggttt cctgctctat tactatgata gcacccgtct tgctttttga
    15181 aagctgcgca acggcaacac atacttcgtc aagcatgctg tctatttcat gttccaataa
    15241 ttctcccttt ataaagaacc tgcctcttcc gatatgctcc agtgcccgtc taagttcggg
    15301 ctggaatatg attaaaattg caataacccc taccgtcatt gcgtttttca acagccagta
    15361 gacagtatga aggcccagaa cctcactgag cttggttgcg ataagaagca ttgctatacc
    15421 cttaatcagc tgctccgccc tggtttctct tattaaattc attgctttat aaataattat
    15481 ggcgataatt ccaatatcta catatgtgtt ccacattttg agatacggga gcagttcctt
    15541 aaactcatcc aatctattca cctcttatgc atgcaatttc tttcatactt caattatata
    15601 ataaattcca taatgaaata ttacaagttt gtttcaattt tttccggata ttccttcatg
    15661 gtatggctgt gcgcggtttc taactaaaag aacaggtggg ttatgtattg gtcaattata
    15721 accaatgccc ctattataaa cacataatag gaaaaatact tcatgctgcc cttctttaca
    15781 agcataatca tgaatcttac cgaaaaatag cctgccgctg ccgcggcaat ggtgcctgca
    15841 acgattggag catttatgat tccggagctt cccgcctcca gaatgtcctt tgcctggaat
    15901 agggcagctc ccaatatggc aggtatcgac agaaggaaag aaaacctggc tgcgaactcc
    15961 ctgtccagat tcctcataag ggcacctgaa atagtaagcc ctgatcttga aattgcaggc
    16021 attattgcgg ccccctgcaa aattcctata aatgcggcat ccaaaaaagt tgtctcctct
    16081 atatgctttc ctccgttgtt aagccttccg gcaaaaagaa taataatacc ggtggcaaga
    16141 aattcaaaac ccagtgtgct gccgctttga aacattgctt caaaggaatc cttgaaagct
    16201 attccgataa ttcctgttgg tatcgtacct gcgatgagca gccaggtaag tctttgaaaa
    16261 ggccttttca gaagggataa tatctccttc cgcagcaccg tcatcacagc tgccaaggtt
    16321 cctacatgaa ccattgtgtc aaacaccaat gtcggttcgt taataccaaa tattttctga
    16381 aacaatacca gatgccctga actgcttatg ggcagaaact ccgtcagccc ctgaaccagc
    16441 cccagtatga ttgcctgaat aatgttcatc gttaaacccc ctatacaaat atccatatat
    16501 tattatgata tattttacgc aatgattcaa tgccgtaaaa taaaatgaat gcgttgcagt
    16561 gcaaagcatc cattcacaac atatattaat acgtattata gccccttgca acgtcaaaaa
    16621 gctgcatcca acatttgatg ctacaagtac taagctaaaa cttcctttca agctgctcat
    16681 caattatttc cattattata tccctgccta tggattccgg cagttctccc gctatcttca
    16741 tagctttgcc tatgaatttt tccgcaatat tttttgacct ttcaattgct ttggaagcat
    16801 ccaaaacctc atacaaattt gcgccctttt cctcttgggt tttgttgatg gcctttattg
    16861 cttcctctct atattctgaa tgaagcgcat acagtaccgg caaagtatat atcccctgag
    16921 cagcatctct tagaagcggc ttgccgactt ttgcctgatt gccggtaaaa tccagaatat
    16981 catcgactat ctggaaagcc ataccaatat tattgccaag aacgcccagt ctattcacca
    17041 gtttcctggg acagcccgct ttataagctc ccatatacaa acttatacca aaaagcgccg
    17101 cagtttttcc ccctatcctt ttaagatact gtttgacgct gacgttggta tcaaacttct
    17161 gctcattctg ggctatttca ctgtcacata tatatgctga agcttttgca atctgtttca
    17221 gcatatcgat atccgcataa tccgctacca gcatgaaggc cctggtaagc aagaaatcac
    17281 cgctgtatac agccacatcc tttcccagaa ccgagtgcac cgtttcattt ccccgcctca
    17341 gctttgcatc atcaattata tcatcatgaa taagagttgc catatggata atctccagag
    17401 cagctgcaat aggcactata gtattgctgt catattggcc aaatgtgcct ccaagtattg
    17461 taaaagcagg cctcagcctc ttgccgccgc tttctgcaaa cctgaccaat gcttccttta
    17521 ctgttttgtt tcttgttctt aaagaatttt ttatagtatc ttctacagca tccatttcct
    17581 tttttaagaa actgttatca ttccacatta gtatcacttc ctattccaag tacatggctc
    17641 attggtttca gggtatattg aactgcaaat ccaataaata aacctgtaat cacagctgaa
    17701 atcaacagcg caggcaagta ataaaaaagc tttgcattct caattatcag actggcaacc
    17761 agtatttgcc cgatgttatg gaatactgct ccaatgacac ttattgaagg cagactgaac
    17821 agtgtcctga atttcctgta catgattgcc atggccatgg tgcttattgt accgccgata
    17881 aagctgtata taaaggaaga tatccctcct ccgaatacag aacctaaaaa agttctcata
    17941 cctacaactg ccagggcttc cttcggcccg aaaagtatta tggcaataag tgaaatggca
    18001 tttgccagcc ccagctttat tcccggaacc ggtacaggca ccggtatcag tctttccact
    18061 aaattaagta ttaatgcctg agagaccaat atccccaaaa gcaccatttt ttttgtcctg
    18121 ctcatattca ttcctccgcc tgttatcaga acgaaatacc gtcgatttcg gattctgctc
    18181 ttccttctat atatacaacg accctgttgg gcaagcatac tatactctgt cccactctgc
    18241 ttaccaatcc tgttttctgg cataccttgt ccgggcatat cacgtccttg acatatgcgc
    18301 cgttttcatc aacgattatg tcaatgtact tgccgttctc aaactgtata tggatttcct
    18361 gctgctccat tcccgtttct acgggtattt ccctataaat atccccgttt gtttctataa
    18421 caatcaatct ctcgtcctcc ggcttgcccg ggttatttat gacaagccat gaaattaacg
    18481 cagcaacaag aattacaatt attatcaggt ctcccttttt tatcttcata aaaagccctc
    18541 tcttatatta catgttcagt gcaaaacata ttgtatatta tacaaccgac aatgtctata
    18601 gtaatagtat ggtataatta ataatatata ttgaatttaa ttatcattat taaaattata
    18661 gttgcaataa tactaatgta tcatatagaa ttgaatatgt aaatttattt gcatttttta
    18721 atatgattat ttttttacta tattttggag gtgcccattt tgattcttga tgcaaaactg
    18781 aggctgacgg ctcttgcttt attatcagtg ctccttttgt ccggatgtca gaaacccttt
    18841 ccaagtgctt ctgccaagcc tctgtccaaa accggcttcc ttctcggaac aattgtagag
    18901 ataacggtat atgataaaaa ggatgaagca ttactggata aggcatttga aagaatacag
    18961 gaaattgagg gcagaatgac tataaataat gcagaaaaca gcgaaatcat atcccttaat
    19021 caagcttcgg gcaaaaatga agtcaagctt agccccgata cctactatgt tgtagaaaga
    19081 ggcaaggagt attcccataa gtccggggga ttatttgata taacaatagg cccggtggtt
    19141 aagctctgga atattggtac tgactatgct gcagtccccg aaaaaaacaa gctggaggaa
    19201 gctgcgggac tggtggatta cagtaagctg agcctgaacc ccgggaacca tacggccttc
    19261 cttgaggaac cgggcatgca agtggatctt gggtcaattg caaaaggcta tgcagctgac
    19321 gaagctgccc gcatattgaa ggaaagcgga gtagagcatg caatcattaa tcttggcggc
    19381 aatgttataa caataggcgg taatccaaat ggcaaccctt ggagaatagg aatccagact
    19441 ccctcaaatc caagaggaga ctttcttggg atagtgcaga ttcaggatca ggcggtagta
    19501 acctccggaa catatgaaag atactttgaa gaaaacggca agatatatca tcatatattg
    19561 aaccccttta cagggtatcc cgttgagaat aacctgagca gcgtatcaat aattaccggc
    19621 aaatccatag atggcgacgg aatttcaact actgtacttc ttatgggact ggaggaagga
    19681 atgaagctgg ttgagagcct tgaaaatgtg gaagccgttt tcataaccaa tgacaaaggg
    19741 gtttatattt cctccggttt aaagaataat tttaaaataa caaattccga ctacaagtat
    19801 agaaataacg gcgaataatc aaaaaaggcg caatacgcct ttttttaatg cctgtcatct
    19861 ccttcatccc taaggtaatc cctcaggaac tcaataagct ctacttcagc cttgctcaaa
    19921 aattcaagac ttctggactc ttcgattatg tattttttaa ggagcagtgc actctggttc
    19981 cagagttcgg gaatatgctt cttcataaga ataatacagt tggccactct gtcatcatcc
    20041 attgtattaa ggcattccat gatttcttca tatttatcct tagaggactc gtctcctgat
    20101 tctgccctgc gtttcagtct tttaacatat ttatgcattt tttcacctgc ctgccaaggt
    20161 ttatcctcac atctccatgc attttaggat aggcatcagc ttaattctca tttatattta
    20221 tatctgcatt tattacaccc ttgagttccc cattcacaat tatgggcatg gaaaccgaaa
    20281 tattgtagtt gccggaagcg catgagatat attcctttga tacgtaggta ttacccttca
    20341 tcgcatttgt aaaatacggt tttgagctgc aatccctgac aaattcaggc agctcggttg
    20401 ttgctcccgt aatgtatccc ctgttgtcaa taattactat aagttctatg tattctaatt
    20461 ttttctgctg ttctttaata tatctctctg ctgaattttt atccaatgcc attatgtttt
    20521 ctttttgtat aatttccctt aataattcct gagtcttatt gatcttttct tttattgcat
    20581 cggtaagaac gaaacccttc ataaaattgc ctattattgc gttggattct tcaagccttt
    20641 ccaaaagcat aacaatatgt ccatgcaccg cttctatggt agccagctgt tcttcagtag
    20701 atgctccaat ctcctccgat accgccgcat tctgctgtgt tacatcgttg accttttgaa
    20761 tctccttctc tatgcttcca atattcttca tctgctcctt tgtaagcatc gttatcttct
    20821 cggcagcctt catgttatcc gtcaaagtac tgtttattac gtcagattta ctgagtacct
    20881 cttccgcaaa cttaagattc ttgtctatag cggatgcctc gtcctttatg ttcaccgaca
    20941 gctcccctat ctggcttgta atgccgtcta ccagctgctt tatctctttc gccgattgtg
    21001 aagagtcatc tgcaagcttc cctacttctc cggcaaccac cgcaaacccc cttcccgcct
    21061 caccggctct ggcagcctct atagctgcat tcaacgccag cagctgtgtc tgggctgcta
    21121 tgctttcaac tgcttctgtt attgtatcta tcgcccttat tttattatcc aggttgtcca
    21181 aagcctccag cacctttttg ttgtattcct tggattcctg tatcttgaac tttatatccg
    21241 aaaacttgcc gaagctgtcc tctattgtgc cttttgtctc ctttgcaatt ttaaggcttg
    21301 tctctgcatt ttcgctgata tcctccgccc cttttgtgaa gtcctgcacc atttccatca
    21361 tgttatgggt atatgcagcc tgattgctta ccgactctgc cacactctgt atcgttgagg
    21421 ctatttctcc cgaagaatac cttattgcct ctgcctgctt ctctacctca tgagcatcct
    21481 tcattatcct tttggataca tcgtcaaaac tgccaatcaa acttctgatc ttaagcaaaa
    21541 actcattcat cgccgaggct atactgtcaa acataatgat accggttttt ttgacgtttt
    21601 tgttcagctg tcctttgttt atttcttcaa tgctgtttcc gatccttgac gaaatactgt
    21661 tcagctttgc caaaatcaga agtcccgttg ctgccatcgc aattatcagt aaaataacgg
    21721 ctaattcgtt accgcgatac ataaacagta cagttccgag aataaggtta attactgtat
    21781 aacccaatag aaagcctgtc attctccaca ctcccccatt gttttgattt tcagttctca
    21841 ttaatattta tatcagctat aataagtccc ccgaatatac cgccccgctg aaagggtacg
    21901 gttacggaaa tattatagtt gtttgttgcc acagatatat attcttcaga tacataagcc
    21961 tctccctggc aagcttttat aaaatatggc ttcgcactgg cgtctctatg gttttccggt
    22021 acttctacgg tagaaaaggt aagatatccc ttgctgtcta tcagacccat gaattctatg
    22081 aaattatatt tctgctgatg ctctcgaaag agtttttcaa cagcattctt atccattctt
    22141 aatatgcctt cgttctttac caattcattg accgcagtct gagtttcctt aaccttttcc
    22201 tttacttcat cggtaagaac gaaacccttc ataaaattgc ctattattgc gttggattct
    22261 tcaagccttt ccaaaagcat aacaatatgt ccatgcaccg cttctatggt agccagctgt
    22321 tcttcagtag atgctccaat ctcctccgat accgccgcat tctgctgtgt tacatcgttg
    22381 accttttgaa tctccttctc tatgcttcca atattcttca tctgctcctt tgtaagcatc
    22441 gttatcttct cggcagcctt catgttatcc gtcaaagtac tgtttattac gtcagattta
    22501 ctgagtacct cttccgcaaa cttaagattc ttgtctatag cggatgcctc gtcctttatg
    22561 ttcaccgaca gctcccctat ctggcttgta atgccgtcta ccagctgctt tatctctttc
    22621 gccgattgtg aagagtcatc tgcaagcttc cctacttctc cggcaaccac cgcaaacccc
    22681 cttcccgcct caccggctct ggcagcctct atagctgcat tcaacgccag cagctgtgtc
    22741 tgggctgcta tgctttcaac tgcttctgtt attgtatcta tcgcccttat tttattatcc
    22801 aggttgtcca aagcctccag cacctttttg ttgtattcct tggattcctg tatcttgaac
    22861 tttatatccg aaaacttgcc gaagctgtcc tctattgtgc cttttgtctc ctttgcaatt
    22921 ttaaggcttg tctctgcatt ttcgctgata tcctccgccc cttttgtgaa gtcctgcacc
    22981 atttccatca tgttatgggt atatgcagcc tgattgctta ccgactctgc cacactctgt
    23041 atcgttgagg ctatttctcc cgaagaatac cttattgcct ctgcctgctt ctctacccca
    23101 tgagcatcct tcattatcct tttggataca tccccaaagc tgccgatcaa gcctctgatc
    23161 ttgagcaaaa actcattcgt cgccgaggct atgctgtcaa acataatgat accggttttt
    23221 ttgacgtttt tgttcagctg ccctttgctt atttcttcta tgttatgctt tatccttccg
    23281 gaaatgctgc ttaatcgggc aataaaaaca aatcctgatg ctgtcattag tattattaat
    23341 gctgcagcgg ccaactggct gctgcgcatt aaatacaatg ccgcaccaaa aactatgtta
    23401 attataaggt ataggctcag taatcccata atgctccaca ctccataatc tcataatatt
    23461 acaaaataat taaataatca attgcatttc acttttttta acaagtatcg gcataataaa
    23521 aagcaggcca tgctgtacat ggctgcatat gcttctttat taattcaatc tgttttcata
    23581 gagtgacaat atatcactaa tgttcttcac tgaattcttt tcactgatat atagcctgac
    23641 cttccctgta agcttcttat tgtatctgat ctttacgcct ttcttaaaaa accttattat
    23701 ctgtttagtc tgctttgctt cattgttatc caatctgata ctcgctgtca tattgccacc
    23761 cctttcaatt aaacattgtt ttcatactat tttaatggtt attatatcat aaattcacta
    23821 ataattgtgt tacatcttaa ttactgccgg cagccacgat atattccgca gcccccagcc
    23881 cctcaaccct gacttccctg tcgtattcct ccggtattat acaagtatcc ccctttttca
    23941 ccgacaattg aaaatcttcg tatcggagca gcaaattgcc cgatactgcg gtaatggccc
    24001 tgaacttgcc cggttcataa aagtggcact cctccttatt gataaatctg aagcttaatg
    24061 caaaatattc acattcaatt atattaataa taccatcctc taatgaatca taaacattaa
    24121 acacacaggg taatcccgaa gagtaattga ttacatccag cgccttgtct atatgcagct
    24181 ctcttccttt tccgcgggaa tctgttctgt tccaatcata aatcctgtat gtcaggtctg
    24241 aaggctgctg cacttcatat gccaatatac cgcttccgat gctgtgtacc gtacctgcgg
    24301 gcacaaatat acaatctcct tttttcacgt ttatatagtt aagtactccg gaaatgagcc
    24361 ccatttccag cagttcccta agctctttct tctgaaccat cctgttgaat ccatatataa
    24421 taaaggaatc ttcctcacac tccaatacta tccaagcttc actcttgcca ttccgtgcat
    24481 tttcaaccct tattgcatat tcatcatcgg gatgcacctg taccgataag gtttccctgg
    24541 cgtctatcag ctttatcagc acaggaattt ctccttccgg tgataccgtc tcccaactct
    24601 cccctgcgtt tttatctccc acatttttat tgtaactgca taatctcctt cctccccaaa
    24661 tcctttctgc tatcatagga tttgtcttgt agggcttcac ttcttcccct cccgataaat
    24721 tgaagtaagg tcttaataat cccctacttc aatttattaa actgtatgga aaagtgaaac
    24781 ccggaaaaat gaaattcaac atgaaataaa gtccggaaca ggctgtctgt taccggactt
    24841 ccgtaattta tcaaattact tgtattaatc agttctcatt tatattaata tccgccatta
    24901 atactccctt gattgtccca ttctgcctga caggcattga cactgttata ttataattat
    24961 tggtaatgac cgatatatac tcctccgatg tatattcttc cccctggatc gcccttgtga
    25021 agtaaggctt tgcactgcaa tccttaaggc tttcatcgac atcctgagtc gctaactcat
    25081 aatagccgct ggagttaatt aatgccataa actcaacata ctcatatctg ctttgctgct
    25141 cattaagata tttttgtacg gaactcttat cagaactaaa caaatctccc ctgctggtta
    25201 tttcctttac caattcctga gttttcttaa ttttctccct tattgaatca ttaagctgga
    25261 agcccttcat aaagtttgat ataatcgaat cggattcttc cagtctttct tttaactgca
    25321 gaatatttcc atggactgtt tcaatggtag ccagctgttc ctgggttgat gctcctattt
    25381 cttccgatac tgccgcattc tgctgcgtca aatcattaat catttcaatc tgcttttcaa
    25441 tgctgtcaat acttgtaagc tgtgtttttg taagtgttgt tatcttccct gccgcctgca
    25501 tattgcttac aagtgtatcg tttataatat cggacttgct taatgcctcc gttgcgtata
    25561 ccatattctt atctatggca tccgcctcat cctttatatt gaccgacaac tcgcttatct
    25621 gaccggtaat tccgtctatc aactgcttga tttcttttgc tgactgagaa gagtcgtccg
    25681 caagcttccc tacttcgcct gctactaccg caaatcccct gcctgcttct cctgccctgg
    25741 ctgcttctat tgcagcattc agcgccagca gctgtgtctg ggatgcaata ccttcaacag
    25801 cttcagttat tgtatctatt gcccggagct tttcatccag gctgtccaga gcctgaagca
    25861 ccttcttgtt gtattccttg gattcttgta ttttaattct tatgtcttcg aacttcctga
    25921 agctgtcctc tattatatct tttgtttcct tcgcaacatt aaaacttgta tttgcatttg
    25981 catttatatc ctcagcatct tttgcgaagt tctgcaccat ttccatcatg ttatgggtat
    26041 atgcagcctg cttgcttacc gactcagcaa cactttgtat cgtagttgct atctcactgg
    26101 aggaatactt gatagcctcc gcttgtttct ccacctcatg ggcatctttc attacctttt
    26161 ttgatacgac accaaaggaa gcaatgaggg ctctcacctt gaataaaaaa tcatttatcc
    26221 ttgatgatat gaggtcaaac atatcaattc ccatcttcct gacgtttttg ttcaactgac
    26281 ctttattgat tacttcaatg ttgtctccaa tcctgactgc tatcccttta agccgtgtca
    26341 tgaaaatgat tccggaagat ataatcaaaa gcattaatat aataacagct aatacattgc
    26401 tgcgcaacat aaacagggcc gctccaagta taacattaac cagtacatag ccagataaaa
    26461 agccggccat ctataactcc cccttttctt gactttcccc tgaaatttcg acatcaattg
    26521 cttcaataca actttattaa tcatataata ttacaatttt ctggaataat caaatgaatt
    26581 tcatattttc cattttttat ctcaattgtt ttgccgaccc ccgcattaaa ggggtaaata
    26641 ccttgccttt gaaacaaata aggcatacag acagccttgt attgctgtag tgtatgcctc
    26701 agaatcactt aatgactata ttcacaagcc tgccctttac tactatcacc cttaacactt
    26761 gttttccggc aatcaatccc tttgtctccg gatttgccat agccatatcc tcaatttcct
    26821 tttctgaagc agcagagggt acttctattc tgcccttgac cttgccgttt atctgtatag
    26881 ccagctctac actatccctt accagagcgc tttcatccca tacaggccat ttctggttga
    26941 aaaccgagta ttcatacccc agcttctccc acatttcctc cgtaaaatga ggtgcaaagg
    27001 gagccagcag cttcaggaga tccgccagga cctcttcaaa gtatggtata ttcttgtatt
    27061 caacttcctg atcatactta tacaatgcat tcaaaagctc cattatcttg gctatggagg
    27121 tgttgaactg gaatatctcc gaatcctttg taacgccctt tattgccatg tgccttacat
    27181 aattaagttc cttttctttc tcagccactt catttcggct tttttgccct ctgagttcat
    27241 atactctctc aaccatcctt tccgctcttc ccaggaacct gataatcgcc tttattccgt
    27301 catcgctcca gggcccccct tcaatatagt tgaagccgaa gcaaagatag gtcctgaata
    27361 cgtcggatcc ggattcattt atatatttgt ccggagaaac gacattgccc ctggacttgc
    27421 tcatcttctc cccgtcagca ccgagtatga ctccctggtg caccaatgac aggaagggct
    27481 cgtcaaaatt caggcagccc atatccctca gggcttttgt aatgaatctt gcatagagca
    27541 gatgcattgc tgcgtgctca gcccctccta catatatgtc tacaggtagt aacttattta
    27601 tccgctcctt attaaaaggt tctttactgt tcttattatc cggatatctg aactgatacc
    27661 aggaggagca tacgaaagta tcaagggtat cgggatccct ctgtgcgggt ttgccgcatt
    27721 tgggacatac ggtattcata aactccttgc tctttttaag aggcgattct ccatcgggag
    27781 taaactccac atcataggga agcattaccg gcaaatcctc ttccggtaca ggcactaccc
    27841 cgcacccttc acagtgtatt atcggtatgg gagtgcccca atacctctgc ctcgatacca
    27901 gccagtccct gagcctgtaa tttattttaa gctctcccct gccttccttt gccagaactt
    27961 cggtgatttt gaccaatgca tcttccgatg tcataccgtc aaattttccg ctgttgacaa
    28021 gcactccgta ttcaacaaag ggcaacttgt catcaacatc cccaacccct ttaattactc
    28081 tgggaatcgg caatccgaac ttttcagcaa actcgaagtc cctttcatca tgggcgggaa
    28141 ctcccattac acagccggta ccataggtgg caaggacata atccgatatc caaacaggta
    28201 tcttttcacc ggatatgggg tgcaccgcat atgcccctgt aaatactccg gtcttttccc
    28261 tggtagttga aagcctgtct atttcgcttg cttttctggc aaaatccttg taagcttcta
    28321 cagcttcctt ctgctcagga gcagtaatta catccaccag ctcctgctcc ggggcaagca
    28381 ccacataagt aactcccata agtgtatcga tcctggtggt aaacgtcttg aagctaaggt
    28441 cttttctgta tattttgaac tctatctccg aaccttccga ccttccgatc cagtttctct
    28501 ggattttctt agtcttttcc ggccagtcga tttcatccag cttatccaga agctcctggg
    28561 cataagccgt gattttaaag aaccactgag tcaggtttct cctgattact tcagtttcac
    28621 accgctcaca atggccttcc actacctgct cattggcgag gacagtgttg cacctggggc
    28681 accagttcac cggtgcttcc ttcctgtatg caaggccgtt ttcgtacagc ttcaggaaca
    28741 accattggtt ccacttataa tatgaggggt cacaggttac tacctcatag tcccagtcaa
    28801 aggttgcccc catctctctg agctgcgctt ccattgtggc aatattattc tttgtggaat
    28861 cccagggatg tattccggtc tttatggcat agttttctgc aggaaggccg aatgcatcaa
    28921 accccatagg atgaaacacc tcatagccct gcatcctctt tatacgcgac caggaatccg
    28981 taggaccgta attataccaa tgccctacat gaagatttgc ccccgaggga tatgagaaca
    29041 tctccaaaca ataaagcttc ttatctgcat tttgtggatt aaacttgtag agccctgttt
    29101 cttcccatct tttctgccac ttcctgtcga tatcctttga atacggcatt aataacactc
    29161 cttccttaac aataaaaaaa cttcatctca ataagagacg aagtatattc gcggtaccac
    29221 tctttttgat ttacggcata ccataccaat aaatccgctt tttcgacggc gcatcattac
    29281 cgtctatcct tttaacgcag gaaatacggc ttaccttact gtcaattcag gaagctgctc
    29341 ccgggcgagt tccaagaact aaaactaccg gttcccacct gtctccggct ctcttgaagc
    29401 tgttcattct tctactattc ccgttccttg catatatcaa tatttacgaa ttatgttagc
    29461 acgttttgat gctcctgtca ataccttaga cgcgagattg tcgccatgta tggcgaccct
    29521 acgtagggac accatacatg gtgtctgcat atacgtttag tatgcctcag aattattgct
    29581 tttatccccg tgtcttgcgt ctcgtatcta ttttatacaa atatattcat gatatcattg
    29641 tttggttcat atttcttgcc ctgccaggta tccctgtcca tcatcaaatg ttctatagcg
    29701 tttatgactt caaatttttt aaggctccat ttcggagcaa taagcgcatc tcttggccca
    29761 tcaccggtta tcctgtgtat caccatattc tccggaattc tttcaataca gtcaactaca
    29821 acctctatgt aatcctccat ggacatcagt tgaaactccc cttgcctgta catttccgca
    29881 agcctggtat ccttaagaag gtgaagcaga tgaagcttta taccctgggt tccggtattg
    29941 gagataaaat caactgtctg aatcatatcc ctcttattct cacccggcaa accaagtatt
    30001 tggtgggtta ccacctctat gttccgcttc ttaagttctt ttacggaagc tatatatgta
    30061 tccagcccat atcccctgtt gataagccgg gctgtccgct catgcattgt ctgcagcccc
    30121 agttcaaccc aaagataagt tctgctgctg atctccgcaa gtaaatccaa tacgtccgca
    30181 ggcaggcaat ccggcctggt tgcaatggca atgcctgcca catcctccag attaagcacc
    30241 gagtaatatt tctcccttaa tacgtctgct tctgcataag tattggtata cgcctgaaaa
    30301 tatgcgatat atctgccgga tttccacttc ttgttcatca tacccctgac ttcatggaat
    30361 tgctcaacaa tattctgaga agtccctgca aagtctccgg atcctctcgg gctgcaaaaa
    30421 atacagcccc ccgaagacac tctcccgtct ctattggggc atgtaaaacc tgcatccaat
    30481 gatattttaa ataccttctc cccaaacttt tccctgagga agtgattcaa ggtgtggtat
    30541 ctctttccgc tccatgtttt gctcactttt ccttcaccct tttacctgat ttgttctgcc
    30601 tgataatcta atagatatcc gagaattcta tgttgaccct gtctacaaaa acacaggatg
    30661 tttcttcggc tgcagccgct atttcatcgt cctccgccaa ccttacgggg agtttgacaa
    30721 taaactcgct gcctttgccg tactcgcttt ttacttctat tgttccttca tgcatttcca
    30781 caagagattt caccagagaa aggcctattc cgctgccctc gcgcttacgg gtaaatgact
    30841 tgtctacctg tctgaacctt tggaaaatta agtccttcat ttcctcgggt atgccgatgc
    30901 cgttatcttt tatagatata tatgcccact tatccttctc gtataatgat atatttatct
    30961 tacccccatt ttccgaaaat tttacagaat ttgaaataag attcagaagt attctctcga
    31021 ttttatcagt atcgcaggct atcttcaggc tatccggttc aatgttcagt tcaagctgta
    31081 tctctttgct ttgcaggaac ttttcaacgg aggctgtaat attttttaca attgccgcaa
    31141 gatcggtgtt atgaagacgc atctcaatat aacccgaatc gatttccgta atatatatca
    31201 aattgttaag cgttcttaac agcctgtagc aattctgcct cataattgca gtatattttg
    31261 ccatcttatc gctgtccgca gtgatgttgt cattgctcaa gccaagctca ataagctgca
    31321 gtgtcccgat gataatatta agaggtgttt tgacttcgtg ggaaatgttg gcaaagaact
    31381 cggttttgac tttgtcgtat tccagggctt ctgtgatgag cttcacctct tcataactct
    31441 tcttcagctc atgctcaacg tttacttgtt ttgtaatatc cctgaagctc catactcttc
    31501 caaccaggct gcccttatcc atcagaggat ttgaaaatac gtcaattatt cttccgtcat
    31561 gcagggaagc aatatagtgt tctattgcac aggtttcata taaagaattt tcccaggctt
    31621 cataaacatc agaatccttt agctgcttcc tgataaaact taaaaactct ctcgcatcac
    31681 tcaagccgca catatcggga ggaatattcc atattttaaa aaacaggtca tttgctttca
    31741 aaatctttcg gttgatgtca actaccagta tgccgtcccc cgttgcatta acggtggcct
    31801 ccagtatttt aaggttatcc gccaaggatg attccgcata ccttcttctg tccatattga
    31861 ttgatataat tactgcggcc gcaaacagca atactagtgt atatgaaatt gcaatcaccc
    31921 aaaatatgtt tctttgtatc tgctgcagtc tgtatgtact attgaaaggg atccctgtct
    31981 gcccgatcct gtacataaat aataaatgta atacaataat tattaaagcc gtcactgccg
    32041 caaaagaaat ccatctgttt ttcaattctg accccccgga taagaaaagt agaaatatta
    32101 tacataacat gttaattttt gtcacttgta tgcaacatat caataacact attatttcat
    32161 agtacaaaat ttatttcaat caacttcgta ttacaatata cgacataaat tttcacagtg
    32221 aattcgactt attccgccgt atattttctg aagaaaaagg gatcccgcca tctactcatc
    32281 cggtagtttg cggcacccct ttaccgtact gcaatattta tattccgtcc atttatacct
    32341 ctgatgaagc tgctttcctg acagccttct gatgattagc atcaaccctg ttggttccat
    32401 gggtcctgct gttcagaaaa tgcacatcaa acacaccgtc cataccgttg tttttaatct
    32461 tgtcaagatt gtatccgcgt ccgtatccct gacttctctt gttcagaatc ttgtttgccg
    32521 gctgactgtc aacacccgca tggggcattg ctgccataga agcagccagc cttcttccgt
    32581 ttaattcaat aataattggc cttctggccc agctccaatt gcccccccaa atgcttttta
    32641 taatttttga atcctctgag gtggctgcct cgcagtcggc atggttgccc ccgtaggtcc
    32701 tcactatctt aaagctcttg cctgtcccta cgtccgtaac ctttgcagta gttccgatac
    32761 taaaaacctt attggcttcc ttccaccaat ccagcatttc aatttttgca tttttgcttg
    32821 atgcggccgc tgtccttgaa gctgcactcc tgctgcttgt cccggattca aacaaaaatt
    32881 tggatgtttg ttttcctaca ataccgtcaa ctgcgatacc tttacttgac tgaaactttc
    32941 tgaccgctgt ttttgtcata cttccaaaat agcccgttgc tttgccgtta aaataaccca
    33001 gctttttcag ctgttcctga agcttggtca cctcggctcc tctgctccca tacttcaacg
    33061 tatctgcaaa ggttaccgat gttaaggata aagttatgac cagaaaaaaa attaaaagct
    33121 tgctccttct cacttactct cctctcccgg cttacgaggt gaatatgtag cacctattgc
    33181 agtacggcgt attttaagtt tacataataa ataccatttt ttcaatggaa ttatgtgcca
    33241 tacacacata ttcccctgta ttgaatagaa tgaatatata tattatttgg ggcgggtgat
    33301 tggatgcttg taaaaaacaa tgcaaattac agagacctta ttgtcggtgc ggataccggg
    33361 ataccgcttt ccaacggtca aatagccgct tccataaatt ttgacaacgc tgcaacaact
    33421 ccgcccttcg catctgtaat tcaggcaata gaggactttg ctccctggta ttcttcgata
    33481 cacaggggaa caggctttaa atccaaatac tcttcagaaa tatacgaaaa ttccagggag
    33541 acaataaaaa aatttgtcaa ggccgatccg ggcagcctgg ttgtttttgt gaagaatacc
    33601 tctgaggcca tcaacaagct gtcttacagg ctttatcaca aaaacaggcg aagtgtgata
    33661 ctatctactt gcatggagca tcattccaat gacctgccct ggagaaacaa atatttggtg
    33721 gactatatca atgtggacaa caatggaagg ctctcaatgc gggaccttga aagaaaactg
    33781 cacaagtacc ggaaaaatct tgctcttgtt acggtaacag gcgcttccaa cgtcacaggc
    33841 tataaaaatc ccatacatga tatcgcagaa ttggtgcaca aatatgattc aaagcttatg
    33901 gtagatgcgg cccagcttat aggccattcc tgcgttgaca tgaagcctga cggaagcagc
    33961 cggcatatcg attatctggc attctccgcc cataagatgt atgcgccctt tggaacggga
    34021 gtgcttatcg gccaccagtc tgattttgaa aagggggagc ctgaatacag gggcggaggt
    34081 accatcaaca tcgtcaccca tgacctgata aaatgggcgg agcctcccca caaggaagaa
    34141 gcaggcactc ctaatataat gggcgttgtg gctcttgccg aagcagtcag gacacttgct
    34201 tctgttggaa tggaaaacct ggaggcctac gagagcagcc tccttgaata tgcccttgac
    34261 agaatttcaa ttattcctga tattaagctg tattgtgatg atgacccaag ggacagagtc
    34321 ggtattatac cttttaatat tgcggggctg caccatcaga ttgtagccga cgctttatcc
    34381 gctgagtcag gtattgcggt aaggaacggc tgcttctgct cccagccata tgtgcagagg
    34441 cttatgaaaa tacccagaaa tgatataaga aagcggataa agaacccttc tcttcctcat
    34501 cccggaatgg tcaggctaag ctttgggctc tacaacaatt acgatgagat agatgtgctt
    34561 gtgaataaac tgtatgaaat atcggcgaat aaggaactgt ataaagaaag atacgggcag
    34621 cacttaacac actgatggct attatcaaat tcatttgaaa ggtatccggt attttcagta
    34681 ttatcaatgc tatttaagag cgggcattcc acatgctgtg atttactctt ttatgcatta
    34741 cttccctgtc ttatggataa aatacgcata tccgaattgg tacagggagg gttattaatt
    34801 gaacaaaaga atgaacatac ttatgttcag cggtgagtac gacaaggcta tggcagcact
    34861 tatacttgcc aatacggcaa gagaaataca ggtggaagtt accatgttct ttgctttttg
    34921 ggggctcttc ctggtgaggg atcctgaaaa gttctccgca aaagacaaga cagcctatga
    34981 aaaaatattc ggggcagtta ccccaaaagg gcctgagcag atgcccctct cccgtatgaa
    35041 tatggcaggc cttggcaaga aaatgctcct tgaaatgatg gatgacgaca gtgctcctca
    35101 tcttattgac ttcctgaagg gagcaaggaa aaaggatgtg aaattctacg gctgcaagct
    35161 ttcaatggag ataatgggcc ttaaacagga ggaaatgatt tccgagcttg agataatgga
    35221 agcaaaggat tatctcaagg atgcactgga atcggatttg cagctgttta tatgagcata
    35281 tacaatattc ttcttctact ctgtcacgcc cgctaaataa ataagaagca ttataaactg
    35341 cttattcagc aactttcgct cgtgcttttt cgacattctt aaaaatgtac ccgtatcagc
    35401 cagaacttga tcttgccgtt gctccagccc aggtcccagg gcactctgtt tctgtcggta
    35461 ttatggcagg ttaccagtgc gtatcccttt gagtcggcgc ctgtaaccac tgaaatatgc
    35521 gtgatatctc cctttttctc ataggcaatg aagtccccgg gcaatagctt gtaggatgcc
    35581 ttatacacct tttcataatt accgtaggca atgagtgatg ccctgccgct gtatagcata
    35641 tagctcttga agccgtctgc gttgacccac gcacgggtag cccccttatt gttgtaattc
    35701 cacgagtggg tttttctgaa cttccctcct tcgtgaagta tttgtgatgc aaaatttgcg
    35761 cagtcgcctc cctgggagtt gtagttcctg tatttcttgt tgtacttgta gccatatttc
    35821 tcctcgctgg cagcgccgca gtatttatct gcataagcta ctgcattttt ccgcctgtcc
    35881 cctatattgg aaaaatccct tgcagcatgg gtgcttatgt actgatgtat tgaatcaacg
    35941 tcaatccttc ccagattcaa ggagtcttca aaggggtcct tataccattc ccttgttatt
    36001 atccagacct catccttgta cagcaggtca agcacatggt aggttcctat cctgaagctg
    36061 tttacaacac cgggctcatt ttcatataca tacttatatt ccgttgaaca catcagatta
    36121 attgtgtatt tttcgccctt cggcttgatg cttctaacct tcaccgtcgg tatgatttcg
    36181 gtgaatttca ccccctgctt cttctcccac ttgtggaggt acttgacctt ggtcagctca
    36241 tgctgatgtg cccatgtacc gtactttgtg cttgtgtcat acagggattg aatcagctta
    36301 aggtcaccgt ttattatcgc attatttctg tttcggaata tctgatgtat ggaatcggtc
    36361 atgtcctctg tttcacttaa tcccgaaaaa acctttcttg cccctgcagg cttctccagg
    36421 catacgaaca gcagcatcac caataacatt agccctatta ctgctttcgt caacttgtct
    36481 gccataatat ctctcattga actccccctt gaatccccca ttcgtttata tataaatata
    36541 cttactccta tttggtttta ttacttgcct tccacataaa aaataaaagg ctgattttag
    36601 cgggatccac taaaatcagc cttatccgga cgccatggcg tcactacttt ttttgtacaa
    36661 tttttaatac tattaatgct attgctcctc ccgctgctgc agttatcagc tgcggaaagc
    36721 tgaaggcgag ccttatgggc gcaggcagct tgtctactat tatatttgcc gctgtataaa
    36781 gcaaaatgta ttttgtcaaa gctgccgcaa ttataccgat ataatcatta tatcttctta
    36841 atagtctgaa caaaacaaca tatacagtat tccccgccat tataaacgga accagcacaa
    36901 ttgtcggagg cggctgcacc ccaagaagca ccgccagcat gggtgtcatt ataccgattg
    36961 ccgccgccca gggcaatccg caaactccaa ctgcagtaat aagtaccgca ttgattcctg
    37021 ttcccgttac atactggccc attttgacaa gctggaatac tatagctata gccagtaata
    37081 ctgctgcctt tgttagcaga ttgagcttcc ctttcattta tcctaagccc tgtccctttc
    37141 aatatacttt gtatgcagca aacgatggga tatatggctg ttgggttcct ccagataatc
    37201 ttcatagagc tttttcactt caggattctc atgggatttc ctgaacctcc tgtcttcctt
    37261 gtcaatctta tacaagcctt gcgctctaag cttcttaact tcctcagtct tttccttatc
    37321 caacagtatg ggctggcctc ctccgttgat gcatcctccg gggcaagcca ttacttcgat
    37381 gaaatgatat tcggccttac cgtcctttat gctgtccaaa agcttagcag cgttccctgt
    37441 accatgggca accgcaactt ttatatcaac tccgtcaacg ttcactgacg cttcttttat
    37501 tccttccatt ccccttacgg gtgtataatc gatctttttg agatcctgcc cggtcaatat
    37561 atccgcaacc gttctcagag ccgcttccat aacccctcct gtagctccga agattacggc
    37621 ggcacctgtt gaatcaccca atatactgtc atatttccct tcactcaaag ccgtaaaatt
    37681 aatcctaacc tgttttatca tcttcgcaag ctctcttgtt gtaagaacca catcaacatc
    37741 cttcaatccg tctaaagcca tttcttccct gtttgcttca aatttcttgg cagtgcacgg
    37801 cataaccgat acaacaaaaa tatttttcgg atctatgcct gctttctccg cgtagtagga
    37861 ttttgctact gcacccatca tctgctgagg tgacttgcaa gtggaaagat tgtccaggaa
    37921 ctcatgataa ttatgttcgc agaattttat ccaacccggt gaacaggatg tgataagagg
    37981 gagcttacct ccgttcttaa tcctgttcag caactcggta ccttcttcaa gtattgtaag
    38041 gtctgcggaa aaatccgtgt caaacacctt gtcaaaaccc aatcttttaa gagctgttat
    38101 catcttgcct tcgacatttg tccccaccgg cataccgaac tcttctccga ggccgaccct
    38161 gactgccggg gcagtctgca caacgacatg caaatcggga ttacccaaag cactccacac
    38221 cttatgagta tcatccttct cccttagagc gcccacaggg cagatcttta tgcactggcc
    38281 gcagtttata caattcactt ctgtaagcgc ttttccatta ggtgcagtaa ctgtcgttct
    38341 gattcctcta ttggcaaaat tgatagcctc tgtatgctgt atatccctgc atatggctat
    38401 acatcttcca cataatacgc atttattctg atctcttact atggaaggag acgcttgatc
    38461 cacagcatat actattttct ccccttcata ggaaaccttg tctattccca tttcatcaca
    38521 gagcttctgt agctcgcagt tcagattcct tgagcaagta gtacattctc tttcatgtct
    38581 tgagagaatt agatccagta ttgtttttct tgcctccata actgcagggg aacatgtttt
    38641 gacctccatt ccctcagata caggcagtac gcaagaagcc tgaagtgttc ttgctccttt
    38701 tacctcaacg acacagattc tgcaggctcc aacctcattg atccctttaa gatggcaaag
    38761 ggtaggtata ttgatatcca gagattttgc agcttcaagt actgtgaatt ctttgggaac
    38821 ctgggtttta ataccgtcga ttgtaatatt gaccgtttcc attgtattat acctccgcta
    38881 aattttttcc ggcaaaggca gctctgcttg ccggagcaat aatactttct atacaaaaaa
    38941 actcctttcc gttccggaag gagtttgata cattaatacg catgctcaaa tacgggcata
    39001 cccattaacc actgctcctt tccaaaaggg aggcaccgac atttctagcg gtaccctatc
    39061 ggtccaaatt ccatagccat atgccttagg aaatttggac tcggagaaat gtgtatatta
    39121 agtttatgtt attgttttat ctataatata tgatacacaa cacaaacaac attgtcaatg
    39181 gtttaacatt gtttggattt attttccata taattgctgc tttgccagtc aaacaagccc
    39241 tttcaaatct tcccgggtgt tcaaatttgc gaacatgctc cagtcggggc taaaaccccg
    39301 tgcaatagct tcatcaatat ataaaacatt agagatttcc aaaagctcca ttgttcttac
    39361 cttgcccttt ttaatattat cttcaatagc gggtataagc tttttactgt aaaaagcatt
    39421 aaagggttct atccaatccg cttttttcgt gattaccgca tcactcttaa aagaatcctc
    39481 ttccaacctc tttatcatat attctatgta gcatttatta atattaggca tgtcacaggc
    39541 aataaaataa ttatacatgc tctgtgattt ggaaagcccc gcatgaattc ccccaatagg
    39601 cccatatcct ttatatacat cctcagctat ggctatattc ttgtctttat acagctcagg
    39661 cttgttcgtt acaacaatta tgtttttaaa cacggggctc agcatttcaa ccaaatgttc
    39721 aactatacag atatccttaa cccttataag ctgcttatca aaacccatac gggtgctttt
    39781 accccctgca agtattacag ccgtatttac caagctcatt atataccccc aaaaaataag
    39841 ttatgccttt gctttcaccg cttattgtac aatacctgat gcttttatgc aatgaaaaag
    39901 ggatgactcc gaatacggaa atcatccctt gaaaactaaa ttatattata ctgctgtccc
    39961 ctgcatatct ttaacctttt ccaccttaac agcagatacc ttgtattctc ctgtcttggt
    40021 taccgggtca aaagccgaat tggtcagttc gtttgccgga gattctctgt aatggaatgt
    40081 catggacatc ataccgggtt ttactttatt ggttattgtc actctggtta ctatagaacc
    40141 tcttctggag gtcaccctta cgaagtcttc ttctttaagt ccgagctttt cagcatccgc
    40201 cgggttaatt tcagccagtt cgtaaggttt tatggcatcc agcttttcag aaaatcttga
    40261 ggtaatattg taatgataca gcatcctgcc tgtgctgaga agtataggat actcttcatc
    40321 tacggcttct gccgggactt tatattcaat tgccttcatt aatgcctttc ctttggggaa
    40381 tgtaccttta tggaggtatt gagtacccgg atgatcttca gtaggacatg gccactgcaa
    40441 tccgttctta tccagtcttg caaaagtcat tcctctgtag ctgggtgtag ttgccgctat
    40501 ctccttgaat atctcttcgg catccttgta gtcaaatcca ccggcgccaa gcccctttgc
    40561 cacatcgcag attatctccc aatccggtct ggcttctccg ggagcttcga ctgcttttct
    40621 gaccatttgt actcttcttt cggtattggt aaatgttcct tccttctcag catagcatgc
    40681 cgcaggaagt actatatccg caagcttggc agtctcggac aggaagatat cctgaactac
    40741 taagaagtca agcttttcca atgcctttct tacatggttt gcatcagggt ctgtaagtac
    40801 cggatcttct cccataatgt acattgcttt tacttttcct tcatgggatc cgtcaaacat
    40861 ttcgggtatc ttaaggcctg tgttcctatt gagcttgaac ttccagaaat cctcgaagaa
    40921 ctggatagcc ttttcatcag taacactctg ataccctgag aatacgttag gcagcgctcc
    40981 catatcgcaa gcaccctgaa cgttattctg tccgcgtata ggattaatgc cggcattttc
    41041 tataccgaga tgtcccacaa gcatagccag gtttgccagg ctcataacat tatctgttcc
    41101 ggttatatgc tcagtgattc ctaatgtgta gaatatacct gctttctttg tgctggtgta
    41161 cagctttgct gtttcataaa gcaattcttc agagattccc gttatctttg acacatactc
    41221 cggagtatat ttttctattg ccttgagtaa ttcatcaaat ccctcacagc gggtttcaat
    41281 gaattcctta tcatgccatc cctcttttac gattatgtgc atcaatccgt ttattaacgc
    41341 aacatccgtt ccggaattca acggcagcca tagtgtagca tattccgcaa gctctgttct
    41401 gcgcggatct atgactataa gctttgatcc gttcctggcg gctctcttca tcttattccc
    41461 tataatagga tgagcttctg tagcatttga acctataatg aacaatactt catttccttc
    41521 aacttcggaa atggcgtttg tcatggctcc gcttcctaat gtagctgcaa gacctgcaac
    41581 tgtcggagca tgtcaggtcc ttgcgcagtg gtcaacactg tttgtaccca ctgccaccct
    41641 catcattttc tggaacaaga aattctcttc atttgtgcag cgtgcagaac tcaagcctgc
    41701 tattgcatcg gggccgtatt tttcctttgt ctccttaagc ttttctgtta ctgtgtgtaa
    41761 tgcttcatcc cagcttattt caacaaattc cccgtttttc tttgccaaag gctttgtaag
    41821 cctttcgggg ctgtttatca tgtcaatatg gtatctgcct tttacgcaaa tggaggttcc
    41881 gttaaccgga gaatctggat tgggtgtaac ccctaccacc tttccgtcct ttatattcag
    41941 gtcaaaattg catcctacac cgcagaaggg gcatgtagta cgtactttgc tcacttccca
    42001 cggtcttgtg cccttcaact gtttgtttat tattgcaccg gtcgggcata cactgatgca
    42061 ctgaccgcat aatctacagt tttcagtact tatcgggaca tcatatgctg ttgctatctt
    42121 ggtctcaaaa cctctgttga taaagtctat agtgcctgta acctgaactt ccctgcatac
    42181 tctgacacat tttccgcata agatacattt gctttgatcc ctttccatta ccggattcaa
    42241 gctttcaata tcatagctct ttttttctcc tttataggag gacctcttaa ttccatatct
    42301 gtatgcatag tcctgaagct tgcaattgcc agcctgttca caagtcaggc aatcctcagg
    42361 gtgacttgcc aaaagcaaat caagaagatc cttacgaaca ttcactacag tttcagactc
    42421 tgtctgaatt accataccct cacttacggt attggtacat gaagctacaa gggcctttgc
    42481 cccctgaacc tctaccacgc atagtctaca tccaccaaat gcagaaaggt ttttatcata
    42541 gcataaatga gggatatcta caccgttttc tttcgcggca tccaaaatgg tagtcccctc
    42601 tttagccgtt actactttcc cattcagggt aaatgtaacc atttcaccac ctccaacatt
    42661 ataataatac tgcattaata tatcagaatc attgttatat aagtaacaaa ttaattatat
    42721 aagagttcaa acctattgtc aactttttat caatagcaaa aaacacaaat agagctgcac
    42781 tgacttccag cccatactgt gtttctatgc ttttatatat tcatttttca gtgtataaac
    42841 tttacctctt ttaacaaaat aatgctgtct cctaagagct ttgcatcttt ttcgccagtg
    42901 acatctcctt aacattcgtt atactattat gagtataacg taattaatat gaaaagtaaa
    42961 gcaaattttg taatatcttc gtgttataat aatagtgtga atttattctg aaaggaatga
    43021 tttaagtaat gtacaatata aaatataaat tatggctgga caaaaatggt gttatatttg
    43081 ggacgggttc ttacttgctt cttaaaggcg taaaggaaaa gggatctttg gcaggagctg
    43141 caaaggccat gaatatgtcc tataacaagg cacataatct gataaaaaag atagaaatca
    43201 ggttgggctt ccggcttctg tacagtaaga cgggaggtgc aggaggagga atctccctgc
    43261 ttactataga ggcggaagaa ttggtgtctg catacgaact gttttataat gaatgtgaag
    43321 aagcaataaa tactatattt aaaaagtatt ttcccgatca ttgaaatttg aaaacattgt
    43381 gttaattgta tataatttat tcgtaaaata aaataatgtt ggggtggtat attgaataca
    43441 gtaaaaacag aaaacgctgt gggaatggta ctgggacatg acattaccga aatcataccc
    43501 ggggagtaca aaggagctgc cttcaagaag ggacatataa taacggaaaa tgacatagaa
    43561 cggcttctgc gaataggaaa ggagcatgta tatgtttttg aacttggtga ggacgaactc
    43621 catgaaaatg atgcggctct caggttggga aaactgtttg caggacccgg catcagtttt
    43681 tcagaaccct ctgaaggaaa aaccagttta aaatcaatgt atagagggct tttgaaaata
    43741 aacagggatc tgctggagca ggttaatgat acggaagata tctgtttggc taccatacaa
    43801 gagaatatat gtgtggagaa ggatgcattg ctgggaggat gcaggattat tcccctttca
    43861 ataaaaaaag agaaaattga acatgtggag aacatggtaa aaggcaagga tccgatgttc
    43921 aaaatcaagt ctttcataaa tctcaggaca gctttgatag taaccggctc tgaagtatac
    43981 aaaggaagga tcaaggatga attcgggccg gttgttgaaa agaaagtaaa acactacggt
    44041 tcgggaattt ttaataaaac catagtaacc gatgaattgg atgaaataaa gaatgccgta
    44101 ttggatgcaa aaaaagcagg tgcggaactc attatagtta cggggggcat gtcagtggat
    44161 ccggatgaca aaactcccgg cgccattaaa gcaaccggag cagatattat aacctacgga
    44221 acttctgttt tacccggtgc aatgctgctg tttgcctacc tggacggtac gcctgtgttt
    44281 gggcttcccg gctgtgtaat gtttgcacat actacagcct ttgacctatt acttcccaga
    44341 gtctttgcag gcgaaaaaat tatcagaaaa gatatcacca gaatggggta cgggggacaa
    44401 tgcctatcct gtgaggtgtg taaattccct tactgcaatt tcgggaagca ttgaggagga
    44461 aaaatgttag aaaatataac cgttgagact gcaaagagaa tattatcgga agaaaaaaat
    44521 gatattaagc aaagcgaact gtacattctt gacagcttgg atcatgtcct tgccgaagac
    44581 attgtatccg aaaccgacat gcctcccttt gacagatcac ccctggacgg ctatgcctat
    44641 aaatcggaag acacagtaaa tgcagccgaa aactctccgg taaccttgga agtaatagac
    44701 tatgtacaag cgggatatgt aagcagaaaa aaaatatgta acgggcaggc aatccgaatt
    44761 atgaccggag ccaaaatacc ggaaggcgcg gatgtggtta tacaatatga agatactgaa
    44821 tttaccgata agacagttaa aatattcaga tatctcaggc cccaatccaa tatagtaaaa
    44881 gcaggggaag atatgcgggc aggagacctg atactgcgca aaggcataac catcgggcct
    44941 gcagagatag gtgtactggc ttcaaacgga aagacccatg ttaaagtata ctccaaacct
    45001 acaattgcaa tactgtcaac cggcgacgag cttcttgaaa ttgatgaaga attggctgac
    45061 ggcaagataa gaaacagcaa ttcctatgta atagcggcac aggtaaaaaa atacggcgga
    45121 aatgccaaaa tactcggaat ttgtatggat agggtaaacg atgttaagaa gaaactggat
    45181 tctgctctca aatgggctga tatgataatc acaaccggag gagcttctgt cggagatgcc
    45241 gatataatca aagaagcctt caaggcagcg ggagccgata tattattctg gaaaacaaga
    45301 atgaagcctg gaaccccaat agtagcggca aagcatggga ataaattgtt attcggatta
    45361 tccggcaatc cggctgcagc atttatcacc tttgaaattt ttgtggggcc tgcaatatta
    45421 agattaatgg gaaaaccaaa gcacaaatat atgaaagttg aatccatact ggaaagcgat
    45481 tttaacaaag tcagcgagca ggacaggtat ttaagggcct tcacatacaa aaaagaagga
    45541 gcctattata ccgtacttcc ggataaacac agctcgggag ttttatccag cctgtcaggg
    45601 aaaaactcat tactgcttat ccccggcgga tcaagccctt ataaaaaagg agaccgggta
    45661 actgttgaat tgatcaacca tctggaggcg gaattatgat accggtgttt tcagttgttg
    45721 caaacaattc taaagtaggc aagaccacag tattatgcga tattatcaag gaattaaaat
    45781 ccagagggta caggatctgc accataaaac atgatgttca cggctttgat attgaccacc
    45841 cgggtaaaga cacctggaaa catgcacaag caggctcaga ttttgtagtt atttcatctc
    45901 caaaaaaatt agcgatgatt gaaaaggttg aaaaagaata cagcctggac gaagtgataa
    45961 gcaagataag caatgtagat ataatcataa cggaaggttt taagcaggag cgcaaaccaa
    46021 aactggaagt ttacaggaaa gagttcccga atgaaatatg gtcaaaagat gaagacttat
    46081 ttgccatcat cacaaatacg cctttaaaca aaggtgtacc ccagttcgat ttctcggaga
    46141 taaaagaagc agtcgactta atagaaagct tattcttgaa ataaacgcct catttgccgc
    46201 cgcactccga tgcctggccc gtcaatatct ctatcccatg cttcaatgcg ggtattattg
    46261 cttcaagatt ctctctcaca ccctttgggc tgccgggaag gttaataatt agggactgct
    46321 tccttatgcc cgcctgtgcc ctggagagca ttgccttcgg ggtagcctga aggctcttcg
    46381 ccctcattgc ctcgggtatc cccggtacat acctttcaat tacctcaaga gttgcttcgg
    46441 gagttatgtc tctttttgaa aagcccgtgc ctccggtagt cagtattaaa ttcaatccca
    46501 gcgtatcgga catgtctatg atagcttcct taatatcccg catttcatca gggacaataa
    46561 catatttttc tatacggcct cctatctgct tcattatttc ctcaatagca gggcctgaag
    46621 catctgctct ttcacccctt gaacctttat cgcttactgt tatgattcct acactgaatc
    46681 cctccattgg atcccacctc ctacactagc atttcgtatt attgatgtct tcgttcaact
    46741 tgccgctgaa attgaattcc cccgacttgc cgcccctttt atgcacaagg tgtatatcac
    46801 ttatcaccat taatttatca atggccttgc acatatcata tatagtcagc gccgcaacgg
    46861 atactgccgt caaggcttcc atttcaactc ccgtaactcc gacagtcttt acaaccgctt
    46921 caatatcaat tttggaattt tcataatcag cctgaaattc aatatctgca ccatatatgc
    46981 tgacattatg gcacataggt attatatccg gtgtagactt ggctcccatt ataccgccta
    47041 cctgtgctac ggataagaca tctccctttc ttatccttcc ctctgcaata gccttcaagg
    47101 tctcccgttt catataaaca gaacctcttg caattgcctc tcttacactt tcttcctttt
    47161 cagacacatc caccatcttg gcatagccct tagcatttat gtgggtgagt tccccaacac
    47221 cctggacttc ctttttattc tttgagttca tacaatcacc tcgttatctc atattttagc
    47281 acaaactcaa gaaattaata agggcagtgc gataatttag caaaaaactt ccatgactaa
    47341 tatcaaaata ttgtaagtat gctaaataat taacattatt tacactatat aaatatgcca
    47401 atttttgata taatttgtca tggatggtat tttatcgcca ttttttcaac tgtatcaagg
    47461 aggacagcag gatgttcaag ataagcagta aaagtataat gttcaaagta ctggtgatga
    47521 tattaaccat atgtatgttg gaatctttcc tttttacaat aatgtcggaa agactgaccg
    47581 gcagcatttt ggaaaacatg accatcgaaa acagcagaaa aaatgcagag ctgtactccg
    47641 aattcatagg cagctggctg ggagaacgaa tgcgggaaat taatatgtat gcaaattctc
    47701 ccgttataag agcaatggat tggaataaca tagaactcta ccttaagcag gaagtcaaag
    47761 agaagcttga tgtatatgac cattttatgg ttgcagaatc aaacggggat tacaatacta
    47821 ctctcagaag aaatgtcgga aacgttagtg accgtgagta tttcaagcat gtcatggaag
    47881 gcaaaacagt agtgtccaac ccgattgtct ccagatctac cggaaagccg gaagcgatta
    47941 ttgcatcacc tatactggac aataacggaa aaatcaaagg agttatggca ggagcagtaa
    48001 atctcatgaa gctttcaagg attattgata atcttaaata caactatccc aactcatact
    48061 catatattgt agacaaagaa ggccaaatca ttgcccatcc cagggaagaa ttaattctcc
    48121 ggcataatat aacaaaggaa tcagatgtca tcagtagaga catagctgaa gcatcggcag
    48181 aaatactcac aaatgaccgg ggctctaata catattcctt tgaaggtatt gtcagcatga
    48241 attattatca tataatcccc aataccgacg gttggaagct gattatcaaa atacctgtga
    48301 actactggca aaccccgatg agatatactt catcgcgtct tatactaatt gggcttgcag
    48361 gccttataat agcttcaata gtcggatttt tcattgcaaa aagcatatca actccaatca
    48421 taaagcttaa agaagtgttt acaagggcaa ccgccggaga tcttacagta aggtcggaaa
    48481 tcagtaccga cgatgaaatc ggagatgccg caagaagctt taataaaatg atggatacca
    48541 taagcaagct gacctattat gattcactaa ccctgctccc caataaactg cattttgatg
    48601 caatactgtg cagggaaatt aagaaatccg ccagtaataa taccatgctt gcattaataa
    48661 tatttgacat agacaggttt gaaagcatta acaatgcttt gggccataca gtgggtgaca
    48721 agcttctgaa aagcctggca gaaaaagtca gctgccttga agataataac catgtagttt
    48781 cccgtatcgg agaggatatg tttgcagtat tgcttaagaa ttttaaagaa aaggaagatg
    48841 ttataaagct gtcggatgaa atccggtata ttatcaggca gccctgggtc atagatgaat
    48901 acagatttta tattactgcc tgcgtaggaa ttgccatata tccgaaagac ggagaaagcg
    48961 gcgacagcct gtttaagaat gcattttcgg caatgcaaaa agcaaaggta cacggcaggg
    49021 ataattttaa tttctacgaa ccttcaataa acgaactgct tgttgaacaa atgaacctgg
    49081 acagcagtat gcatcatgca ttggacaacg gcgagttttc catatactat caacctcaga
    49141 tggatacgcg cacccaagag ataattggct gtgaagctct gttgagatgg aatcaccctg
    49201 agctgggaat gattggcccc aatgaattca tccccatctc tgaggctaac ggattgataa
    49261 cccttatagg cagatgggtt ctctacacgg cttgcatgca gaacaagctt tggcaggatt
    49321 caggcttcaa accaattttt gtatcggtta atttatctcc tgcacagctt atacaagaga
    49381 acttcatcga tatggtatcc gaaattcttg aagaaacaaa acttttgccg gaatatctgg
    49441 agcttgagat tacagaaagc gttgctgtaa aaaactacga ttacataacg ggtatattgg
    49501 aaaagcttaa gaaaatgggc ataagaatag ctcttgatga ttttggaacg ggatattctt
    49561 ctcttaatta tctcaagaat tttgccatca ccactttgaa gatagaccgc agctttatcc
    49621 atgacataaa tgaaaatcca aagaattcag caattgtatc cagcatactt gcaatgggca
    49681 ggaatctgaa gctctgcata accgcagaag gaattgagac taaagagcaa tatgagactt
    49741 tgagagacag gggctgtgac atcatgcaag ggttctattt cagtaaaccc cttcccctgc
    49801 atgaatttga aaaattaatg aaaaaacccg gtgattaaga ggacatgggg acggggacac
    49861 ggggacgttt cccctgtccc accggaagtg ggacacgcga aacgtcccca tgtcc
//
LOCUS       DAOOAB010000015        47826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_927_length_47826_cov_15.047581, whole genome shotgun sequence.
ACCESSION   DAOOAB010000015 DAOOAB010000000
VERSION     DAOOAB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 47826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47826
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_47826_cov_15.047581"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1541)
                     /gene="spoIVB"
                     /locus_tag="PKU88_05000"
     CDS             complement(237..1541)
                     /gene="spoIVB"
                     /locus_tag="PKU88_05000"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HPX68676.1"
                     /translation="MHKKNYRNVFLYTTLIITILCLTNVLFTIYSFPDEINVIQGQDR
                     KLPSSMVCNLEVIGDYKHSIDVNNNKIISSFLLMEDAGKGKAKANLMLLGLLPVKQVT
                     LNMIPDIKVVPSGEAIGVRIESKGVLVVGLSSITDTKGRRCSPAADAGFEVGDKIIQI
                     ADNQVEKERDIIDYLNNRHDKEERTKVLVERDGSRQDLYVKPVKCEEDGIYRIGLWVR
                     DNIAGVGTMTFYDPKSRMFGALGHGITDVDSGVLVDINSGKIIKSKIASIQKAKKTVP
                     GELVGVFYDSEDEYGIIYKNTSFGIYGKLSKKQKPERTKPVSIGLNSQIKEGPAKILT
                     TIEGNKVEEFDIEIQKAMRQRNSESKSMIIRITDEKLIEKTGGIVQGMSGSPIIQDNK
                     LVGCVTHVLVNDPTKGFGISIEWMLKEADIELVDSLKRASGE"
     gene            complement(1703..3385)
                     /gene="recN"
                     /locus_tag="PKU88_05005"
     CDS             complement(1703..3385)
                     /gene="recN"
                     /locus_tag="PKU88_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPX68677.1"
                     /translation="MLLEMSIKNYVLIERQLIAFEPGLNVITGETGAGKSLVIDALAA
                     VTGGKFSKEDIRTGEEKASVEALFSLEGVWDIKNILEEYGIEKDADNSLVITREVNAH
                     GKSVCRLNGQIVTLSVLKRIAQYLVDIVGQNEYQLLFNSVKHGEYVDSFGSDEIYQIK
                     RDVKHLSDKHAELSEKLKSASGSASERERKLDMLRFQVEEIESARLKINEDEELRLRR
                     HLLVNAEKLLKGVNHVYNVIFSGDSNKRGALDMLSESAGTLRELAAADEKLETYKSRI
                     EEIMYQLEDIKGELRVYRDNIEFDDNEIDFLEERLDQINKLKRKYGSSIEEVLVYRDN
                     AAKELQELIDNEKNMAEIESEIEKVKDQYMIKARLLSGTRKAKAEILQKLIEKELQDL
                     NMYGSRFVIKILQDENIISPNGIDKIEFMISPNPGEPEKALSKIASGGEMSRVMLAIK
                     NAFSEVEKAPCVVFDEVDAGVGGQTANMVGQKIKAISKNAQILCITHLPQIASLTDNH
                     IFVDKKVQDNKTYTEIKILNFEERVEEIARMLGGNENIEASLEHAKQLVGKM"
     gene            complement(3397..3846)
                     /locus_tag="PKU88_05010"
     CDS             complement(3397..3846)
                     /locus_tag="PKU88_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPX68678.1"
                     /translation="MKISRHAKILEIIEKHPTETQEELAEELKKCGYNITQATVSRDI
                     KELKLVKVLDEDGIYRYASLKEQDSMLNERLVKVFAESVLSVDFAGNIVVVKTFSGAA
                     NAAAEAIDVLDFKEVVGTVAGDDTIFILIRNLENVEVVIDRLKKMMK"
     gene            complement(3873..4721)
                     /locus_tag="PKU88_05015"
     CDS             complement(3873..4721)
                     /locus_tag="PKU88_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPX68679.1"
                     /translation="MKLGIIPNINKDKDLNVTRSILLWFANKDAVLMLESDIAEKLGY
                     KNSGFSREKIYSSSDVVIVLGGDGTLLNIARQSARYDVPLFGINLGHLGFLAEAEISD
                     MYLSLDKIMEGDYNIEKRMMLEALVEDDENNGKEFLALNDFSVTKGMTSRMITISTYI
                     NDSFFELYSADGVVISSPTGSTAYSLSAGGPIVSPELKVLIITPICPHTIHNRSIVVS
                     EKDEVKIVIGDENPEVMLSVDGQDSYRLNSGKVVRIRSSSFVTNLVKLKQRSFFDVLR
                     RKISER"
     gene            complement(4811..5623)
                     /locus_tag="PKU88_05020"
     CDS             complement(4811..5623)
                     /locus_tag="PKU88_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPX68680.1"
                     /translation="MAEKERLDVLIHKCGMSESREKASSLIMSGIVYVNGIKVDKPGT
                     KLATDSNIEIRGNALPYVSRGGLKLEKALCEFGINLKGKVAIDIGASTGGFTDCMLQK
                     GALKVYAIDVGYGQLAWKLRTDSRVVCMERTNIRYVAPEDISESADFSSIDVSFISLT
                     KVLPAVMQLLNEKGEIVCLIKPQFEAGREKVGKKGVVRDKDVHIQVIEKIISFCRDEM
                     GLSILGLSFSPIKGPEGNIEYLLYLSKRKDLSDTAQRIDIGGIVLKAHELLR"
     gene            complement(5630..7480)
                     /gene="dxs"
                     /locus_tag="PKU88_05025"
     CDS             complement(5630..7480)
                     /gene="dxs"
                     /locus_tag="PKU88_05025"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPX68681.1"
                     /translation="MLDSINSPADLKKLSYQELEVLSKELRDYVIKVVSENGGHLASN
                     LGVVELTLALHRVFDTPGDKIVWDVGHQSYIHKIITGRRESFKTLRKINGISGFPKRE
                     ESVYDSFETGHSSTSISAALGMAKARDLAGENYSVAAVIGDGALTGGMAFEALNHAGD
                     FPTNLIVILNDNKMSISHNIGGLSNYLSKLRTEPLYFKMKGRIEAFLHRIPVIGGSMY
                     RGAERLRDWMKYLLVPGVIFEELGFKYFGPINGHDIRKLEDVIKRAKSYNGYPVLIHV
                     VTTKGKGYSIAENKPDIYHGISPFFIENGELKSKNSRKTYSEIFGDTMLKLADKDNKI
                     VAVSAAMAEGTGLSAFASKYKDRFFDVGIAEQHAVTFCAGLASEGFRPVFAVYSTFLQ
                     RAYDQIIHDVCMQKLNVVFAIDRAGIVGSDGETHQGVFDITYLRHIPNIRIMSPKDGN
                     ELSEMLKMALSLEGPTAIRYPRGKAKKFEFAHAPLELGKSEVLTEGEDGVIISEGNMV
                     SIALEVCEKLKKAGRNFTLVNARFIKPLDEELLVGLSKKHKLIYTLEDNVVSGGFGSS
                     ILELLSRLRADVDVRIIGFDDSFIPHGEKEELYSLKKIDSESVFNAIMKE"
     gene            complement(7516..7962)
                     /locus_tag="PKU88_05030"
     CDS             complement(7516..7962)
                     /locus_tag="PKU88_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPX68682.1"
                     /translation="MNFFVKVADNKALLACITAWLIAQILKIIFTFYSSKKLDLTRLV
                     GSGGMPSSHTAFVMALSTSIGRMYGWDSPIFAVSLCFAFVVMYDAAGVRRAAGNQARI
                     LNMIIEDLAHNKPITDEKLKELIGHTPKEVMAGALLGIVVANIMVR"
     gene            complement(8020..8901)
                     /locus_tag="PKU88_05035"
     CDS             complement(8020..8901)
                     /locus_tag="PKU88_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPX68683.1"
                     /translation="MDLKEYLANKRHLVEQKLKSMIPADNSIPVIMEAMRYSLLAGGK
                     RLRPILAIMSCELFGGQEEEVLPFACCIEMIHTYSLIHDDLPAMDNDDLRRGRPTSHK
                     VFGEGFAVLAGDALLNSAFETMTAILMDNPKPEYIRAMNIICRASGISGMIGGQCIDL
                     YYENRSIDIETLKSMHRKKTGALIKASLAAGAVIARADEGDIKNIMEFGDAIGLAYQV
                     EDDILDVSGSEEKLGKSINKDAEFHKSNFISFYGLDKSISIAAELIGEAKKKLERYGD
                     KSCYLIELSDYIIDRDN"
     gene            complement(8904..9140)
                     /gene="xseB"
                     /locus_tag="PKU88_05040"
     CDS             complement(8904..9140)
                     /gene="xseB"
                     /locus_tag="PKU88_05040"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPX68684.1"
                     /translation="MNENFSFEQALKRLEEIVETLEAGNISLEESIKIYQEGIALSKL
                     CSGKLEEAEGKVMAIMNRGSEQMEEFSISQIKEA"
     gene            complement(9190..10401)
                     /gene="xseA"
                     /locus_tag="PKU88_05045"
     CDS             complement(9190..10401)
                     /gene="xseA"
                     /locus_tag="PKU88_05045"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPX68685.1"
                     /translation="MNNERIFTVTEVNNYIKHLIQNDVLLSHLHVKGEISNFKHHSSG
                     HIYFTLKDADSRIRCVMFKGNASKLKFMPEDGCAVILRGYFSIYERDGQYQFYTEDMI
                     PEGTGSLYKAYEQLKKKLAAMGYFDEARKKKIPFMPRAVGVVTSPTGAAVRDIISIIR
                     RRCPCIDVYLYPVQVQGAGAENEIAEGIRYFNSKGNVDVMIIGRGGGSIEELWAFNEE
                     VVAKAVYESDIPVISAVGHETDYTISDFTADLRAATPSAAAELAVPDMQSLLGKLSQY
                     QYVLNSAIANFIKDNRSRVERISGDMVFRQLERKVLNMRQTLDSLDKYLDYNMLNIMK
                     GRRNEIDKTIEKLQLLNPEAAMGRGYGIIMRKSDGKLLTSVKDMKLNEELYIKLKDGK
                     AEVRVKGLEST"
     gene            complement(10394..10852)
                     /gene="nusB"
                     /locus_tag="PKU88_05050"
     CDS             complement(10394..10852)
                     /gene="nusB"
                     /locus_tag="PKU88_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPX68686.1"
                     /translation="MSRRLAREFAIQFLYSTDFNRNENTDEMLEDFYEIKKEQGKDGQ
                     QDSMKEADMKFAEEIIKGTIGKLQEIDQFIQNNTTGWTKERIAKVDLAILRLALYEIL
                     FRDDIPDSVSINEAIELAKKYSTDESGSFVNGVLGKIIREADIKVYGTNE"
     gene            complement(10942..11337)
                     /locus_tag="PKU88_05055"
     CDS             complement(10942..11337)
                     /locus_tag="PKU88_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPX68687.1"
                     /translation="MEKEIKLDKYGNVKIADDVVAIIAEIAAREVQGVYSMSGGIADS
                     ITEILGKKNPSKGVKVEVGEKEAAIDLYIIVDYGVRIPDVSWQIQENVKKAVETMTGL
                     TVVEVNIHIQGINMDKESKEEENPEGKSK"
     gene            complement(11492..12076)
                     /locus_tag="PKU88_05060"
     CDS             complement(11492..12076)
                     /locus_tag="PKU88_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HPX68688.1"
                     /translation="MQVKKKNLIICSMIILLFVVGYAYNSFTSERLTDANLDLDAQVI
                     GTDEMGMPAETAGTEEAAGPIVIEDSGSEALETTAASFFSEYRIERDKNRSKEVEMWQ
                     DIINSDKAEENFKNMAQQEIVKIVSLTDKEMIIENLIISRGFNDALVFLTDDSATVIV
                     EAKELTSSNIAQIQDIVVRKTKLDPKNIKIMKKN"
     gene            complement(12107..12730)
                     /gene="spoIIIAG"
                     /locus_tag="PKU88_05065"
     CDS             complement(12107..12730)
                     /gene="spoIIIAG"
                     /locus_tag="PKU88_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HPX68689.1"
                     /translation="MKLHEWLMDKLRKVNFQKLNFSKKTTANLIIVFCLGLALILIAD
                     FYRDIRRDRASEEGIFSEITQADTGTSDNNASDYVRRLEADLSSILSKIQDAGKVSVM
                     ITLESGAEIIPAKDESTSDKVTNEKDTSGGTRIINEKTTDDKVVLTAAQGGNSKPLII
                     KEINPEVKGVIVVAEGAKDSKVKLKISQAVQTVLDIPAYRVTVYERN"
     gene            complement(12752..13387)
                     /gene="spoIIIAF"
                     /locus_tag="PKU88_05070"
     CDS             complement(12752..13387)
                     /gene="spoIIIAF"
                     /locus_tag="PKU88_05070"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HPX68690.1"
                     /translation="MADFLREWIINITVIIIFIMFLDTIIPNSSMKRYINVIVGLMII
                     VVVIKPFVLIKDYADSFNMKFLETSSYIEQGGSAADSTEISNFQRQMAVEIFENNLKK
                     KVGRIVDNSTDSDYEEVSIDLRLEKDFESKDFGEIKSIEVIISKRPKEVIEVDRIKIA
                     ADEDAEVNKNVINKDKVEYNLNNSRIRSEIRDGISKALGISESIIGVEVRQ"
     gene            complement(13405..14565)
                     /gene="spoIIIAE"
                     /locus_tag="PKU88_05075"
     CDS             complement(13405..14565)
                     /gene="spoIIIAE"
                     /locus_tag="PKU88_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HPX68691.1"
                     /translation="MGKGIVFFMIIILLSLCAANCDDTQDILQSQLDNLDISDIEKFV
                     DDINRESEGMLPEIDVREMVLGLFKGNTQNSIRDMGKGIASLFFKEVISNFSLMGKLL
                     ILAIFCAILQNLHNAFEEDTIGRLAYGMCYILIIILAVKSFNDAMLIGVDTINKMTGF
                     MQALLPTLLALLASVGGISSSAVFQPLMFTCIALITTFIKVFVMPVILFSAVISILNN
                     LSDSVNISKLASLLKQIAVGALGLVLTAFTGVLTVQGIASASIDGATIRTAKFAVDNF
                     IPIVGNFLSEAFDAVVSCSMILKNGIGIAGFIILLLICSFPLIKMLSVVLIYKVTSAL
                     IQPILDNQIVQCLNDMGNAMLVLLTCVLAVAVMFFMALTVIMGVGNMAVMVR"
     gene            complement(14577..14963)
                     /gene="spoIIIAD"
                     /locus_tag="PKU88_05080"
     CDS             complement(14577..14963)
                     /gene="spoIIIAD"
                     /locus_tag="PKU88_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HPX68692.1"
                     /translation="MDIMKIVMVGIIAALLAVVLKEEKPEMAFAISIVTGLVIFVFVI
                     TKLNSVMTVLKHFAAKANIDVLYFTTILKVIAIAYITEFGAQICRDAGEGAIAAKVEF
                     AGKVLIMVIAIPILAALMDIMIKIIP"
     gene            complement(14973..15167)
                     /gene="spoIIIAC"
                     /locus_tag="PKU88_05085"
     CDS             complement(14973..15167)
                     /gene="spoIIIAC"
                     /locus_tag="PKU88_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HPX68693.1"
                     /translation="MDVDLIFKIAAIGIVTAVLNRVLNQSGREEMAMMTTLAGVVVVL
                     MMVISLISNLFNSVRTMFHL"
     gene            complement(15191..15709)
                     /gene="spoIIIAB"
                     /locus_tag="PKU88_05090"
     CDS             complement(15191..15709)
                     /gene="spoIIIAB"
                     /locus_tag="PKU88_05090"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein SpoIIIAB"
                     /protein_id="HPX68694.1"
                     /translation="MLKIIGGLLVISASGLLGMVYSYKLSLRYRELANLRRLMQMLET
                     EVTYGATPLPAALFSVSKKAEGPIGSFFSCISRCLSDRSFYTVREAWNDSAESILTQS
                     SLKMADIDLIKSFGSILGCSDREDQKKHFQLFYLQLSHQEAAATEEIKRSAKMYRSLG
                     FLLGIAVFVVLA"
     gene            complement(15702..16709)
                     /gene="spoIIIAA"
                     /locus_tag="PKU88_05095"
     CDS             complement(15702..16709)
                     /gene="spoIIIAA"
                     /locus_tag="PKU88_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HPX68695.1"
                     /translation="MKNDIKILEVLEYMPKHIGAMIAKLPEKILMDAEEIRLRLAKPL
                     CVAGSNGELFISADGYAVSEAEEAYLVSEEDLKRALQLTCNFSLYSVEEELRNGFITV
                     SGGHRVGVCGRAVVDSGKVKTMKDISYMNFRVAKQVLGASDKVSGYLVQPPDSIFSTL
                     IISPPQCGKTTLLRDLVRKLSSGSRDIPFKGMKISLVDERSEIAACSMGIPRNDVGIR
                     TDVLDGCPKAEGIIMVIRSMSPEIVAADEIGRAEDADAITAAVNAGVKVITTIHGSNI
                     TDFLNKQDLKRIQKGVFERYIVLSRKKGVGTLETVLDGEFNVLYERSKSICKWGDRVD
                     A"
     gene            complement(16847..17266)
                     /locus_tag="PKU88_05100"
     CDS             complement(16847..17266)
                     /locus_tag="PKU88_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68696.1"
                     /translation="MEYLYERVAYLKGLAEGMKVDESTDQGKLITKIIDVLSDFADAV
                     NELNESQNELDEYVEAIDEDLSALEDDLCYDEDDEEEFDDEEDYDYIEVECPHCKEFV
                     YLDEDLVESGEQVICPNCHEPIEFEYECGCGCDHDQE"
     gene            complement(17333..17890)
                     /gene="efp"
                     /locus_tag="PKU88_05105"
     CDS             complement(17333..17890)
                     /gene="efp"
                     /locus_tag="PKU88_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPX68697.1"
                     /translation="MISASEFRKGLTFEMDGQIYTIVDFQHVKPGKGAAFVRTKIKNI
                     ITGGVTERTFNPTDKYPKAHIERKSMQYLYEDGGLYYFMDNETYEQIPLNHEQVEDAI
                     MYLKPNDTANIKFYKGEAFSVEPENFVEMEVTQTEPGFRGDTATGATKPAIVETGATV
                     YVPLFINTGDRIRIDTRTGEYMERV"
     gene            complement(17958..18839)
                     /locus_tag="PKU88_05110"
     CDS             complement(17958..18839)
                     /locus_tag="PKU88_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPX68698.1"
                     /translation="MIHFGPSGNSDSFYEQGYKSSLDMPAWLRDMGLDAYEYSCARGV
                     RIKPDMAERLGKAARENGITLSVHAPYYINLASEEPEAVKKSINYIIDTSITAELMGA
                     MRVVLHPGSLGKLTRNKAFENIRSNLFRVLEIINKKGIKVSLCPETLGKRNQMGTLDE
                     ILELCLLDELLIPTVDFAHLHALGSGALRKREDYEKVFDRIFTVLGEKRGRLMHMHYS
                     RVEYTEKGGEKKHWSYDDVEYGPEFEPLAECLIKFGISGTIISESRGTMAEDALKIKK
                     IYGRLSKENLYAMEKLC"
     gene            complement(18942..19214)
                     /locus_tag="PKU88_05115"
     CDS             complement(18942..19214)
                     /locus_tag="PKU88_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HPX68699.1"
                     /translation="MYKLKNYMEDLVIKRTDEILKSIDVCKCDKCKLDIIALALNSLP
                     SKYVVSAKGELYAKVSELEQQFDIDVGTAIIKAAFLVNENPRHNEE"
     gene            complement(19240..19905)
                     /locus_tag="PKU88_05120"
     CDS             complement(19240..19905)
                     /locus_tag="PKU88_05120"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPX68700.1"
                     /translation="MESQEGLLIMHGLTVISSSAFTAASVLLVKLLERKVGAGKGDKI
                     GLSFAAPVFILFLSVNLYMINKNAEGSYLILNVLLLTAVVSSYQDIKYREIEDEIHIA
                     ALGAGLVGLLIKGFQPADAFLGLMAGGGTLLLIAILTKGGMGGADIKLNAVYGAILGL
                     KLSMLSLVIAFAAGALASLLLILFGIKKRKDTIPFSPFLSIGALTAFVFGKSIMGIYL
                     GFF"
     gene            complement(20039..20545)
                     /locus_tag="PKU88_05125"
     CDS             complement(20039..20545)
                     /locus_tag="PKU88_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HPX68701.1"
                     /translation="MKNRLKPDIYLNSVYELDTASLKEHGINAIIVDIDNTLVSWNTR
                     EPDEKANEFMCRLGSEGFKICILSNNSRKRVEEFNKCLNLPAIHKATKPSKFAFRRAM
                     KLMKSKPADTAVIGDQLFTDVLGGNRLGLFTILVSPISNKEFIWTRIVRKLEKHVLRK
                     YRQTNNMQ"
     gene            complement(20624..21331)
                     /gene="sigK"
                     /locus_tag="PKU88_05130"
     CDS             complement(20624..21331)
                     /gene="sigK"
                     /locus_tag="PKU88_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HPX68702.1"
                     /translation="MLLFLASLIVFTIKDLCLLFGYIQNVNSFPQPLTTEEEQYYLEE
                     YKRGNEDAKNILVERNLRLVAHIVKKYVNTGREIDDLISIGTIGLIKAIITFDESKGT
                     RLATYAARCIENEILMTIRASKKTKTEVSLHDPIGIDKEGNEISLIDILGSDSDIILD
                     EVELKIQIKKLYQRMDTALKKRERLVIELRYGLLNGISKTQREIADMLGISRSYVSRI
                     ENRAIKKLHKELYNENG"
     gene            complement(21472..23247)
                     /locus_tag="PKU88_05135"
     CDS             complement(21472..23247)
                     /locus_tag="PKU88_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPX68703.1"
                     /translation="MAGIMKFLRKKAVPECRKEEENRSMELLTLPGTRLYRKNNYILI
                     HKKRLQVIGLLFIAACGLLLCRLFYIQVYMGKHYSERAVRQRMVHIPISSNRGIIYDR
                     NMIPITDREVKKVVIAYPDYVYDKKTAVETISKACGIKMEDVEKKMGSADTAEFICET
                     EQNEYLSLIESGRIRGVIAVEKRLRYADDNIARHVVGYIGKTDKTGQMGIEKSMNSYL
                     ESRGSDSIAAVVDSGKNIIRGLGFRKVEAAGDGVSYSLKLTLDYHIQNIIETAMEKND
                     VEGSIVVMDVKNGDILGMASKPDFDQGNVDKYLKSSGNELINKSIWQFDLGSIFKTVV
                     AAAAIEGGYVNPEEKYKCRGYADVGSSRIKCSTHKTHEDIEINMKEAFALSCNSTFIQ
                     IGTATGAENILKMAGRLGLGQKLCFTIPEEKAGYIPEAFEDGIGNISIGQGKIQVTPL
                     QVTCMMAAIANNGIRNDPQIIDSLITEKGLTVKKLERSRPQIVLNPITSKLLKDMLHE
                     VTVTGTGKQANMDEYGGCCGKTSSAQTGIKEGEVVHGWFAGFIPYEKPKYAITVFIYN
                     GQSGGGAAAPLFKEAASKILTAVKR"
     gene            complement(23300..23950)
                     /gene="udk"
                     /locus_tag="PKU88_05140"
     CDS             complement(23300..23950)
                     /gene="udk"
                     /locus_tag="PKU88_05140"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010173838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPX68704.1"
                     /translation="MSEEKHRKPLVIGIAGGTGSGKSTVSKAITDSIPGKKVVIIEQD
                     CYYKDQSHLPPEERVNVNYDHPLAFDNELLIAHMLKLISGQAIDKPQYDFTVHNRKKH
                     TLRVEPKDVILLEGILILDSPRLRELMDIKIFVDTDADVRVIRRIVRDMRERGRSLES
                     VITQYLNVVKPAHNEFCEPTKRYADIIIPEGGYNKVAIDIMVTKIKSIIDGGCDSN"
     gene            complement(23943..25178)
                     /locus_tag="PKU88_05145"
     CDS             complement(23943..25178)
                     /locus_tag="PKU88_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPX68705.1"
                     /translation="MNKPELLAPAGNLEKLKMAIIYGADAVYIGGEKYGLRAAAGNFS
                     LDEIKEGAAFAHDNCRKVYVTVNIIPHNEDLEGLEEYLTSLGDAGADALIVSDPSVIM
                     TAREVLPDMQLHLSTQASNTNYMSARFWHSQGIKRIVLARELSFEEIRETIEKSPKSL
                     EYEVFVHGAMCISYSGRCLLSNYMTGRDANRGECAQPCRYKYYLVEEKRPGQYMPVDE
                     DERGTYIFNSRDLCMIEFIPELAESGVKSLKIEGRMKSSYYVASVVRAYRMALDSYFS
                     NPGNYEFRKEWLEELSKASHREFGTGFFFGKPDSRGQIYESGAYVRDYAFVGLVLDYD
                     SKTGIATVEQRNKMLVGDEIEVIGPRREMFTQKIEKMWNDEGVEILSAPHPQQIVTIK
                     MERPVEKHDILRRERKDGE"
     gene            complement(25171..25836)
                     /locus_tag="PKU88_05150"
     CDS             complement(25171..25836)
                     /locus_tag="PKU88_05150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPX68706.1"
                     /translation="MANISHDYIDSFLLGMIPGRSGILKEMEEYARENHVPIITPNIA
                     SLLAVLIKTAKVKSILEIGTAIGYSAIHMGLCAGKDFSITTIERNEEYAALAADFIRK
                     AGMGDNIRIITGAAEEMLKDIPGSFDMIFVDAAKGQYMDFMKKSIGKLRIGGLYVCDN
                     VLFRGMVAERSLLIRRKITIVKRLKKFLSFITSCESLETAVIPMGDGISVCCKLKEVE
                     FIE"
     gene            complement(26095..27117)
                     /gene="mltG"
                     /locus_tag="PKU88_05155"
     CDS             complement(26095..27117)
                     /gene="mltG"
                     /locus_tag="PKU88_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPX68707.1"
                     /translation="MKAKLKFNIFRFLVFCSLLALVLAYVYYNNGMEAVSGEGSDFKE
                     IVIPKGSTIRYISKILKEEDLIKDSLIFELYCKINEKADKIKAGRYSISSSMKVSEIV
                     DVLVSGKALIDTVKFTIPEGYNLAQIAERISSLGVVSPESIQAALKAEGYEYEFIEQI
                     PDRENKLEGYLFPDTYEIYRDTTAEAIIKKLLGRFDDIFTEKFRSRAEELNMSIDQVV
                     TLASIIEREAKLDSERKTISGVFHNRLKKKMLLQSCATVQYLLKEPKEELLYEDLEID
                     SPYNTYKYAGLPPGPIASPGLAAIEAALYPENTDFLYFFALEDGSHVFTKTYNEHINT
                     QNKYRK"
     gene            complement(27296..29116)
                     /gene="typA"
                     /locus_tag="PKU88_05160"
     CDS             complement(27296..29116)
                     /gene="typA"
                     /locus_tag="PKU88_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPX68708.1"
                     /translation="MEKTIREDIRNVAIIAHVDHGKTTLVDGLLRQSGTFRANENVME
                     RVMDSNDLERERGITILAKNTAVIYNNIKINIVDTPGHADFGGEVERVLKMVNSVLLI
                     VDAAEGPMPQTRFVLKKALELQLKPIVVINKIDRPDSRIEEVIDEIINLFIELGADDN
                     QLEFPIVYVSAKQGVAKLDIKDESQDLRPLFDTIISYVEPPKGDAEGPFQMLVTTIEY
                     DEYVGRIAIGRIERGRIISGMSAALCKRDGVLQSVKLSRIYSFMGLKRKEVEEAFLGD
                     IIAVSGIDDINIGETICDIQNPEAIPFVNIDEPTISMTFSVNNSPFAGREGDYVTSRH
                     LRARLFKELETNVAMRLTETDSPDTFEVAGRGELHLSILIETMRRQGYEFQVSKPNVI
                     MRNINGAACEPIEHLIIEIPEEFMGVVMDRLGPRKAEMMNMGNSSDGYLRLEFKIPAR
                     GLIGYRSQFMTDTKGNGTMHHVFYGYEEYKGEIPDRPRGSLIAFENGETTAYGLYNAQ
                     ERGYLFLGPGVQVYEGMIVGENAKSDDIEVNVCKKKHVSNMRASGSDEALRLTPHKEM
                     SLEECLEFIAADELVEVTPKSIRLRKRILDSSLRAKARNK"
     gene            complement(29144..30064)
                     /locus_tag="PKU88_05165"
     CDS             complement(29144..30064)
                     /locus_tag="PKU88_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPX68709.1"
                     /translation="MDKICIVVNPVSANKTTAREWPLFEKVLSDAGFEFEAVLTEYPG
                     HAAELTRKALKLGYNTIMSVGGDGTMNEVVNGFFENDRLIDENSRLVVFSRGTGCDFV
                     RSLGINRNIEDLLAILKRNREKQIDVGRLSLVRTEGNMPARYFLNVADTGIGAETANR
                     VNMHSKRLSGFLSFMLGTLSTVVLYKNQNFEVVIDDKIHLNGIMNSVIVANGKFFGGG
                     MMVAPDAVLDDGVFDIIILGNLSKYDIIKSFPLIYKGKHMNHPKLKYCRGSKVKVTSG
                     GKGLIEADGEIPGRADAVFELMPKALKILI"
     gene            complement(30071..31759)
                     /locus_tag="PKU88_05170"
     CDS             complement(30071..31759)
                     /locus_tag="PKU88_05170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPX68710.1"
                     /translation="MKGLIKKVAKSKAKLKLIPLGGLNEIGKNMTAVEYKDTIVIIDC
                     GLMFPDEEMLGIDLVIPDVTYLVKNKEKVKAILLTHGHEDHIGALPYVLPQINVPVYG
                     TKLTLGLVENKLKEFGLLSLVERIIVKPKDMVKFGELEVEFIKTSHSIADSVALAIHT
                     PVGIIVHSGDFKIDYQPIDGELIDLHRFAELGKQGVLVLMGDSTNVERPGYTMSESTV
                     GATFMELFRDAGGRVILATFASNIHRIQQAINAAAMFNRKVAVVGRSMVNVIGVATEL
                     GYINIPEGVMIEVDHIDKYPNDRIMILTTGSQGEPLSALARMSTAEHRKVTIIPGDRV
                     VISANPIPGNEKLVSRVINQLFKKGADVIYESLVDIHVSGHAYQEELKLLQALIKPKY
                     FVPVHGEYRHLTQHAQLAEKMGVNKENIFIADIGTVLEFTKDSGRTAGTVVAGKVLVD
                     GLGVGDVGNIVLRDRKHLSQDGLIVVVVTISKETGQVVAGPDIISRGFVYVRESEDLM
                     EEARGVIKDALAKCEEKQITEWGTIKSEVRDSLREFIYERTKRNPMILPIIMEI"
     gene            complement(31937..32371)
                     /locus_tag="PKU88_05175"
     CDS             complement(31937..32371)
                     /locus_tag="PKU88_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPX68711.1"
                     /translation="MREFEYLREKLKEKGFKLTPQRRAILDIILETEGKHLSAEEIYD
                     LVKVKCPDIGLATVYRTMQMFEEVGLVYKHNFDEVRSRYEINHHNEYHQHHHLICVGC
                     SKVIEVEEDLLEQLESGIEKKYNFKISNHNVKFFGYCEKCRK"
     gene            complement(32483..32668)
                     /locus_tag="PKU88_05180"
     CDS             complement(32483..32668)
                     /locus_tag="PKU88_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68712.1"
                     /translation="MVDMKNGLKCGKCGYTLKEQSKCPRCGTVAQCSLSCPGNCLKCF
                     AEAAKANKQKLVDKNAE"
     gene            complement(32656..34497)
                     /gene="feoB"
                     /locus_tag="PKU88_05185"
     CDS             complement(32656..34497)
                     /gene="feoB"
                     /locus_tag="PKU88_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPX68713.1"
                     /translation="MSGCNNCNKNCIKDIKDKKLVLAGNPNSGKSVFFNHLTGIYVDV
                     SNFPGTTVDISTGKYKGYTVADTPGVYGVSSFNEEETVARDVILEADIILNVVDSVHP
                     GRDLFLTKQLIDMGKPVLVALNMIDELQKNGMDIDVKLLEELLGVPVFPTSAVKNIGV
                     TDVLMSLEQARQGNRDPGLTEMMKQFGDTCSEAEALMILEDDEVVAARYGIETSGLRD
                     EIYMERRRAVEEIVNKVLKETSKGASFGILLGRWMLRPVTGIPMLLLTLYAMYKIIGI
                     FVAGTVVGFTEELIMEGMYKPFIISVLSLFISEGSFIGNMLIGEFGILTMVPVYIIGL
                     LLPLIIGFYVLMAVLEDSGYLPRIAALADRMLISLGLNGKAIIPIILGFGCVTMATMT
                     TRILGTKREKIIATVLLGLAIPCSAQLGVIMGLVSGLDVFYIITYITVITSIFVLAGT
                     VLNKLMPGESSHLLIDLPTIRLPMPGNVVKKTFQKTVMFLKEAVPLFIYGALLISVLE
                     QTGALSYIQQWMTPITEGLLKLPPETATAFIMGIIRRDFGAAGLTSIAMTQQQLLVSL
                     IVMTLFVPCIAAIMVIFKERGTRDALAIWLGSFAAAFITGGIVAWLI"
     gene            complement(34526..34744)
                     /locus_tag="PKU88_05190"
     CDS             complement(34526..34744)
                     /locus_tag="PKU88_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPX68714.1"
                     /translation="MTLDKVARGNKVRITKINNRDMKTQAIRIGLYEGADFLCSERLA
                     GGPIILRSRMQEVAIGRRLAEGIEVEKI"
     gene            complement(34917..39536)
                     /locus_tag="PKU88_05195"
     CDS             complement(34917..39536)
                     /locus_tag="PKU88_05195"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX68715.1"
                     /translation="MKHLFRKTTALFLTLCMIVGMTGFPITAYAEGLDSGDGGAADKA
                     APKIGFSVTDSVYGVTELKEEKLSMLFSPMSAVEWVGIGDENSPYQVSSAEHLADIPN
                     QGMGAHYIQTADINLTGINWTPIGDNSSFDSTTQFTGTYDGNNKTISNLTINNTELYY
                     VGLFGYAGSGGTLENIILENVSINSSRDSAFVGGLTGQNCGGTIKDSCVSGSITATGD
                     SARAGGLIGDSYLGGTVENSSSSCAVTGGEGVSNTIGGLVGRQQGGSIRNSYSTGNVT
                     GTYYVGGLVGYLLGSGTIEHGYSTGNVTGTPSDGTIGGLVGTVNNGTITNSCSTGNVN
                     GTNNASNYGIGGLVGYFYGSGGIANSYSTGSVSLLNSGSDTNQGGFIGLIQSTSATIA
                     YCYSTGDVTPGDGSVGGFIGLVRDNPTVTSSYYLDTAAASSARGTALSATALKQRESF
                     VDWDFDTDWKIGEGVSSPTLRWQPWTTDEKITAAHDALDWDAIRGANTLESDVTADLV
                     LNASGAQGASISWSAAPAGFINTETGEVTTPLYSQGDQTVTLTAAISHADGGTPQTKD
                     FTLTVKNVPVTWVGSGDADSPYQVSSAAHLDEIRIKGLGAHYIQTANIDLTSINWTPI
                     GDNVDQFTGAYNGNGYTIANLTINDDGQNYVGLFGSVGTGGTLENIRLTGVNISSITW
                     GAYVGGVTGVNQGTVTNSSSEGSVSGGDSANVGGLIGNGGGVITYNYSAANVTGGNNA
                     NVGGLLGRSYVSTSDNYSTGTITGGDNARVGGLIGDVDDGTVTNSYSTGSASGGTGAS
                     LGGLIGGGNATVNDSYYLDTAGTSAGGDPLTAVQMKQWDSFEGWNFNTIWKIGQNISY
                     PTLQWQTWTADEEITAAVAALAWTDIQGTNSAENDVTSALVLIISGGRDTVISWSADP
                     TGYINTSNGTLSKPYTDEVIVTLTATVSKGSGIPQTKPFSLTIKPLTPDEAITLDKAA
                     LTWAVIKGTNSTEDHVTADLELPDTGTSGTTAITWSADPVGCIDISDGKVTFPDDGNK
                     AVTLTAAITRDGGTAQTKDFTLTIIAHYPVTFTAGTGGSITNGTSGSYAAGTVIEISA
                     APDSGYQFDKWITGSGGSFGNAASASTTFTVPANAVTVTATFTRSSGDRDDRGNKNAP
                     KAAEPEYKAEVKNKDGEEAVLTVTVDKNGGTASVDAGLQSFDIDGSVITIPTVSDIEA
                     YSVSIPMTSLSTADAQGTLTVNTDKGSITIPSNMLTGIPGISGSKAQISIGQGDKNIL
                     PDAVKAAVGDRPIIRLTVSIDGKQTDWSNPNTPVTVCIPYTPAAAELANHESIVVWYI
                     DGSGNAVSVPNGRYDPATGTVTFNTTHFSHYAIVYVHKTFSDLCSAEWARKPVEVMAS
                     KGIINGTGNDTFSPAASITRADYLVLLVKTLGLAAEFDSNFDDVDKSAYYYDAIGIAK
                     KLGIVAGSGNNRFNPRESISRQDMMVMTARALERFKGLKVLDTNGVPEKFADKEDIAE
                     YAVNSMSALITEGLIAGTGNKLNPCAYATRAEAAVFLYNIYSNYP"
     gene            complement(39736..40665)
                     /locus_tag="PKU88_05200"
     CDS             complement(39736..40665)
                     /locus_tag="PKU88_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX68716.1"
                     /translation="MRPELIEFNDGIPVRAFVRSGNQYPYHWHDALEIIQVLKGSVDI
                     GMGDDDLVLREGDIAVTNINEIHCMTGDGEDEILFLHIDSGFCRSVLPNHYMFIYCCS
                     PYHEAQAPEKYGVLKEYIARLVGMLGSSPNRIGQEALRKLLMEMLSYTAYSFDFLRWG
                     FGTEPFDEKRVARLRQMAQNATSDSEVKKGLTVLAAELGVSLQHLSSDIKEKFRVPFQ
                     ELLCYSRCERAARLLLGTDMRIVEIALDCGFSDVKYLIKYFRRFFHATPSGFQKLHRA
                     DSAALARQTQYSDMPLGHAVRLLSAEADGDHIE"
     gene            complement(40851..41132)
                     /locus_tag="PKU88_05205"
     CDS             complement(40851..41132)
                     /locus_tag="PKU88_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPX68717.1"
                     /translation="MSDEMDIILLYDENDNETEFEVVATLEVDENEYAILLPRDDTRD
                     PDAEEVDEAYVLRIEQDENGEDILIGIDDEEELNTVIEAYEELVREESN"
     gene            complement(41209..41634)
                     /gene="ruvX"
                     /locus_tag="PKU88_05210"
     CDS             complement(41209..41634)
                     /gene="ruvX"
                     /locus_tag="PKU88_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPX68718.1"
                     /translation="MRIMGLDLGDATIGVAVSDELGMIAHGITTIRRKSIYYDIDCLK
                     NIICEKDIKRIIVGLPKNMNNTIGPRGEKTIEFVRILEENFPDIEVEMWDERLTTCAA
                     ERTLLEADVRRKDRKKVIDKLAAVLILQCYLDNMKNDNP"
     gene            complement(41734..42681)
                     /locus_tag="PKU88_05215"
     CDS             complement(41734..42681)
                     /locus_tag="PKU88_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPX68719.1"
                     /translation="MDYRILGSTGLRVSRLCFGSLTIGPLQAGLDIEEGASVIRAAFD
                     MGVNFIDTAELYRTYDYIRKAAEGRRKDIIISTKCYAYTREGAEKSLRKALDELNTDY
                     IDIFSLHEQESEHTLKGHQEAIEYFVRAKEKGYIRSFGISTHAIAAVRASLKYKDIEV
                     LHPILNKRGLGIIDGDVNGMLEAAAEAYKAGKGIYSMKPLGGGNMIKDSSQCFDFVLS
                     NDNLHSIAVGMQSYEEVASNVMIFEGKQVPEDIRLKLEKKKRRLLIDYWCSGCGSCSG
                     KCAQKAISVEKGKAVVDSSKCVLCGYCSAYCPDFCIKVV"
     gene            complement(42684..44801)
                     /locus_tag="PKU88_05220"
     CDS             complement(42684..44801)
                     /locus_tag="PKU88_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68720.1"
                     /translation="MNKSLIAFFSIAALSAAIIALQDVKAYEIFASGEEEYGKVVMIV
                     GDYLDIKDIGRMESLYRLAGNSRVALINNRQPGKADAAKSKLIIGSGKKLELDADMVS
                     EGRRESLTGSLVYTGINRIKDRNADSEYKKYIGYIGDAINKKGGTACYIGNADAEKPN
                     KSSMLIAADSSGEIDSGETEDVIIEDAAFPGGKRTDYDRLAELYKQYLPASSFIVIET
                     GDMERLEAFKGSMSEEACQSCKQDVLERIDGFVKKIVSYGGFKTLVFISTYPSKSNAE
                     AGNKLTPIVVYEGREGVLYSNNTRRSGIILNTDIADYVLCKLGYLTECAVEELDKKAS
                     LNALTYMNMSILRTSVLRTPVLTYYAVSVIAVLGILFGAAVFLGKKAGHFWSGFGIII
                     AYTMLSIPIAFLLISASYTDWGSAEYWVLTAAASAVLSILLHLTLRNRLKVILAICLL
                     LQAGLAADILTGGELIKKSVLGYDPIIGARFYGIGNEYAGMFIGISLMVFGCVSELRG
                     RRCGKAAAAAYFSFCTLLLGLTSLGANFGGAVAGVFGYLLAYFLVFDIKFNRRNIFTG
                     ALILGSAAAALFIADSLGLARQSHMGALIKDTRANGVGVITSVISRKISMNLRLMRYT
                     IWTKVLLCIIGAISIMFYKPVKQMFYIFNRYKYLKYSWMSIAASAAAGFAVNDSGIVV
                     AATAMIFMAFTMLIMCIGEGKAV"
     gene            complement(44829..45086)
                     /locus_tag="PKU88_05225"
     CDS             complement(44829..45086)
                     /locus_tag="PKU88_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HPX68721.1"
                     /translation="MGERFEDTMKFKVETDKKYEASDILLSVYSALKEKGYNPINQIV
                     GYILSGDPTYITSHNNARSLIKKLERDELLEELVRAYVDGK"
     gene            complement(45175..>47826)
                     /locus_tag="PKU88_05230"
     CDS             complement(45175..>47826)
                     /locus_tag="PKU88_05230"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPX68722.1"
                     /translation="QDIISVSHARDMRDKAGGVTIKQGYSMPEEDKQLIAAAARIVLK
                     GKYGLLSVQTKVRNGKNAFEYKIWKSKGKKHNKYDMKKLEIKNFNGFGGFDKEGKEYV
                     IKLTEGVNTPVPWCNVISNQKFGFLITESGASTTWAENSRENRLTTWSNDPVIDPSGE
                     IIYMRDEETGEIWSVTPHPIRTKTPYIVRHGFGYSVFEHNWNGIEHELTEFVPVSDTV
                     KLCILKLRNGSNTKMKLSAIYYARTVLGVYEQGTVQHVYTELHSNGAILARNNYNTDF
                     PGRIVFLDTNAKERYYTGDRREFLGNIGGLKHPEALRKDKLSNTLGAGFDPCMCLQTV
                     IELKPREQTEVVFMLGQGESLEAVSAYSERYRNVKRVYKALDDAKAFWEDILRKVTVL
                     TPDENMNILLNGWLLYQTLSCRIWARTAFYQSGGAFGFRDQLQDAMAVIDVLPDIARK
                     QILLHASHQFIEGDVQHWWHPGSNKGIRTRFSDDLLWLPYVTVNYINRTGDVQILEER
                     VEFLEDEPLREGEDERYKIPCVSDVSASLYDHCVRAIERSLKFGHHGIPLMGSGDWND
                     GMSTVGNKGKGESVWLGWFLYDILMNFSELCRMRRDLNKADRYINIAKEIAKAQEENA
                     WDGNWYRRAYFDNGTALGSAENTECKIDSIAQSWSVISGAANLDRAREAMQAVEQYLV
                     KYDEGIVKLLTPPFDEGDLEPGYIKGYPPGVRENGGQYTHAATWVIMAYSIMKNPDMA
                     WKLFNMLNPINHANTMMEASRYKVEPYVMAADVYAVPPHTGRGGWTWYTGASGWMYNV
                     GMQHILGFRKKAGRIVMEPCIPRDWPEYNIRYRHNKNTIYNIIVRNQNRSGNAKAEII
                     LDGRKLEGNEIPLAEDGAEHTAEVILT"
BASE COUNT        11887 a        12398 c         8478 g        15063 t
ORIGIN      
        1 aataaatgca gcatgtttat gccattttct acaaaaatcg actttcgcaa tagaaaaata
       61 atgcgtcggg gacacgggga cgcggggaca tggggacgtt tcgcgtgtcc cactgaaagc
      121 gggacagggg aaacgtcccc atgtcctcgg gagaaacgtc cccgtgtccc cggcgcattc
      181 ttcttgtatc gcgaaaagga gtgccgcagt tctgcggcac tccggcttgc tgaaatttat
      241 tctccggaag cccttttgag gctgtctacc aattcaatat ccgcctcttt gagcatccat
      301 tctatagaaa taccgaagcc ttttgtcgga tcgttaacaa gcacatgggt tacgcatcct
      361 acaagcttgt tgtcctgaat aatcggactc cctgacatgc cctgaacaat tccgccggtt
      421 ttttctataa gtttttcatc tgttattcga attatcatgc tcttggattc tgagtttctt
      481 tgtctcattg ccttctggat ttcaatatca aattcctcta ccttatttcc ttctatagtt
      541 gttaatatct ttgcgggccc ttccttaatc tggctgttca aacctataga aaccggtttt
      601 gttctttccg gcttttgttt ttttgaaagc tttccatata tcccaaagct tgtattctta
      661 tatataatcc cgtattcatc ctcgctgtcg tagaatactc cgacaagctc tccgggtact
      721 gttttcttcg ccttctgaat tgatgcaatt tttgatttaa ttatttttcc gctattgata
      781 tccaccaata cccctgagtc aacatcagta atgccgtggc ccagagcacc gaacatcctg
      841 cttttcgggt cataaaaggt cattgttccg acgccggcta tattgtccct tacccacaaa
      901 cctatcctgt atatgccatc ctcctcgcat tttaccggct tgacgtataa atcctgcctg
      961 cttccgtctc tttcaaccag aaccttggtt ctttcctcct tatcatgcct gttgtttaaa
     1021 taatcaataa tatccctttc cttttccacc tgattatcgg caatctgtat tattttgtca
     1081 cccacttcaa agcctgcatc tgcagcaggg ctgcatcttc ttcccttagt atcggttatg
     1141 ctggataaac ctacaaccag cactcccttt gattctatcc ttacgccgat agcttcccct
     1201 gacggcacta cctttatatc aggaatcata ttcagagtaa cctgttttac cggaagtaat
     1261 cctaaaagca ttagattggc ttttgccttt ccttttcctg catcctccat taaaagaaag
     1321 gaagaaatta ttttgttatt attaacatct atgctgtgct tatagtctcc aatcacctca
     1381 agattgcaaa ccatgctgct cggcagcttt cggtcctgtc cttgtataac atttatctca
     1441 tcaggaaagc tataaatagt gaataataca tttgtcaggc agagtattgt tattatcaga
     1501 gtggtgtaca aaaaaacatt tctgtaattc tttttatgca atagcatcac tctcctgttt
     1561 ccccagaaga gcccgccttc aatcaaaaac ctctctcatt taagttaacc tttttaaagt
     1621 cctattattc tcataaaaat aatgaaccca cgtaagcttt taacttggaa aatgaaaata
     1681 aatacaaatc gggaaaatgt ttttacattt tcccgactaa ttgtttagca tgttccaggg
     1741 atgcttcaat attttcattt cctccgagca ttcgcgcaat ttcttccact ctttcttcaa
     1801 agttcaaaat tttaatttcc gtgtaagtct tattatcctg tactttttta tctacaaata
     1861 tatgattatc tgtaaggctt gcaatttgag gcaggtgtgt gatgcaaaga atttgtgcgt
     1921 tttttgaaat tgctttaatt ttttgcccca ccatattggc tgtctgaccg cctactcctg
     1981 cgtctacctc atcaaataca acgcatggag ccttttctac ttccgaaaaa gcatttttga
     2041 tagcaagcat tacccttgac atttctcctc ccgaagctat tttcgacagt gccttctcag
     2101 gctccccggg attcggagat atcataaatt caatcttgtc aatgccattt gggctgataa
     2161 tattttcatc ctgcaaaatt tttataacaa atcttgatcc gtacatgtta aggtcctgaa
     2221 gctccttctc tataagcttc tgcagtattt ccgcctttgc cttccttgtt ccggaaagca
     2281 gccttgcttt tatcatgtac tgatccttga ctttttctat ttcactttct atttccgcca
     2341 tattcttctc attgtcaata agctcctgaa gttccttcgc agcattatcc ctgtaaacga
     2401 gtacttcctc aatgctgctt ccgtattttc ttttcagctt gtttatctga tcaagccttt
     2461 cttccaaaaa atctatttca ttgtcatcaa actcaatatt atcccggtat accctcagtt
     2521 ctccctttat atcctcaagc tggtacatta tctcttcaat ccgggattta taggtttcca
     2581 gcttttcatc tgctgctgcc aattctctca aagtacctgc gctttcggaa agcatatcca
     2641 atgctcccct tttattgctg tctccgctga aaataacatt atatacatga ttcacacctt
     2701 taagcagctt ttcggcatta accaggagat ggcgtctcag cctcagttct tcatcttcat
     2761 ttatcttcag ccttgcgctt tcgatttctt caacctggaa cctcagcatg tccagctttc
     2821 tttctctttc cgaagcactg ccgctggcgc ttttaagttt ttccgaaagt tcggcatgct
     2881 tgtccgataa atgctttaca tccctcttga tttgatatat ctcatcactt ccgaagctgt
     2941 caacatactc tccgtgcttg acggaattga aaagcagctg gtattcattc tggcccacta
     3001 tatccaccag atactgtgca atccttttaa gtacggaaag agtaacaatc tgaccattca
     3061 acctgcagac cgatttgccg tgtgcgttta cttcccgcgt aataaccagg ctattgtcag
     3121 catccttttc tataccgtat tcctccagga tattcttgat atcccaaaca ccctcaagtg
     3181 aaaacagcgc ttctacagag gccttttcct cacctgtcct tatgtcttcc ttactgaact
     3241 ttccgcctgt aactgcagcg agagcatcaa taacaagcga ttttcctgct cccgtttctc
     3301 ccgtgattac attaagcccg ggttcaaagg caatcagttg tcgttcaatc aggacataat
     3361 ttttgatact catttcaaga agcattactg catcacctat ttcatcatct tcttaagcct
     3421 gtctattact acttcaacat tctccaaatt tcttattaga ataaaaatag tatcatcccc
     3481 ggccacagtc cctaccactt ctttgaaatc caatacatca attgcttctg cggctgcatt
     3541 tgcagctccg gagaatgttt tcacaactac tatattccct gcaaaatcaa cacttaaaac
     3601 agattcagca aataccttca caagacgttc attcagcatg ctgtcctgct ccttcaggga
     3661 tgcatacctg tagatgccat cctcatcaag tacctttacc agcttcaatt ccttgatgtc
     3721 ccttgaaacc gttgcctggg taatgttata accgcatttt ttaagttctt ccgccagctc
     3781 ttcctgtgtc tccgtagggt gtttttcaat aatctccaaa atctttgcat gccttgatat
     3841 tttcatttct ctacctccac tccattttct aactatcgct cagaaatttt tcttcttagt
     3901 acatcaaaaa agcttctctg ctttagtttg acaaggttgg taacgaagct tgaacttctt
     3961 atcctaacta cttttccgga atttaatcta tatgaatcct gtccgtccac cgaaagcatg
     4021 acctcagggt tttcatcgcc tattactatt ttgacttcgt ctttttctga cacaactatt
     4081 gacctgttat gtattgtgtg gggacatata ggagttatta tcagcacctt caattcgggg
     4141 cttactatag ggcctcctgc ggataacgag tatgcggtgg atcctgtagg gcttgatatt
     4201 actactccgt ccgccgaata cagttcaaaa aaactgtcgt ttatatatgt agaaatggtt
     4261 atcattcttg aagtcatgcc ctttgtaacg ctgaaatcat taagagctaa aaattctttc
     4321 ccgttatttt catcgtcttc aaccaatgct tccagcatca tccttttttc aatattataa
     4381 tcaccctcca taatcttatc cagtgacaaa tacatatcgc ttatttcggc ttctgcaaga
     4441 aacccaaggt gtccgagatt tatgccgaac agggggacat cataccgggc tgattgcctt
     4501 gctatattca ataatgtgcc gtcaccgcca agaacaatta ccacatcgct ggaggaatat
     4561 atcttctccc tgctgaagcc ggaatttttg tatcccagct tttccgcaat atcagattcc
     4621 agcataagca ctgcatcttt atttgcaaac cacagcaaaa tgcttctggt tacgttcaag
     4681 tccttatctt tatttatgtt cggtataatc cccagcttca ttaactgcaa caccacctaa
     4741 atgtcttaaa tatattttat attatgtatt tgaaattatc tacaaatatt tacattatat
     4801 aattcccgaa tcaacgcaaa agttcatggg ccttcagcac aataccgcca atatcaatcc
     4861 tttgagccgt atcggataaa tctttccttt tcgacaggta cagcaggtat tcgatatttc
     4921 cttccggccc ttttatgggt gagaagctta accccagtat ggatagtccc atttcatcac
     4981 ggcagaaaga aattattttt tcaattacct gaatatgtac gtccttgtct ctcactacac
     5041 cttttttccc tactttttcc cgccccgcct caaactgcgg tttaataagg catacaatct
     5101 cacccttctc atttaaaagc tgcatcactg cgggaagcac ctttgtcagg gatataaagg
     5161 aaacgtctat tgatgagaag tctgccgatt cgctaatatc ttcaggagca acatacctga
     5221 tgtttgtccg ctccatacat accactctgc tgtcggtcct cagcttccaa gccagctgtc
     5281 cgtaaccgac atctattgca tatactttta aagcaccctt ctgcagcatg cagtccgtga
     5341 agccccctgt agatgctcca atgtctatag ctaccttacc cttaaggttg atgccgaatt
     5401 cacatagagc cttttccagc ttcaggcctc ccctgctgac ataaggcagg gcattacccc
     5461 gtatctcaat atttgaatcc gtggctaact ttgttcccgg tttgtcaacc ttgatgccgt
     5521 taacatatac aatccctgac attatcagac tgcttgcctt ttcacggctt tcggacatac
     5581 cgcatttgtg tatgagaacg tccagccttt ccttttccgc caatgcactc tactccttca
     5641 taattgcgtt gaatacactt tcactgtcta tttttttcag ggaataaagc tcttcctttt
     5701 caccgtgagg tatgaaactg tcgtcaaagc ctattattct gacgtcaaca tccgccctca
     5761 gccggcttaa caattccaga atgctgcttc cgaacccccc gcttactaca ttgtcctcca
     5821 aggtgtatat gagcttgtgt ttcttcgaaa gtccgacaag cagctcttca tccaggggct
     5881 ttatgaatct ggcgttaacc agggtaaagt tcctgccggc tttcttcagt ttttcacata
     5941 cctccagggc tattgaaacc atattccctt ccgatataat gacaccgtct tctccctctg
     6001 taaggacctc gcttttcccc aattccaggg gtgcatgggc aaattcaaac ttctttgcct
     6061 ttccccttgg ataccttatt gccgtcgggc cttcaaggga caaagccatt ttcagcattt
     6121 cactcaactc attcccatct ttggggctca ttatcctgat atttggaata tgcctcaggt
     6181 atgtgatatc aaatactccc tgatgagtct ccccatcgga gccgacgata cctgccctat
     6241 caatggcaaa cactacattc agcttctgca tgcagacatc atgaataatc tggtcataag
     6301 ccctttgcag gaatgtggaa tataccgcaa aaacaggcct gaaaccttct gaggccaagc
     6361 cggcacagaa ggttaccgca tgctgctcgg caatacctac atcaaaaaat ctgtccttat
     6421 attttgatgc aaaggcggat aaacctgttc cttcagccat tgcagccgac acggcaacta
     6481 ttttgttgtc cttgtccgcc agctttaaca tggtatctcc gaatatttca gaataagtct
     6541 ttcttgaatt ttttgattta agctctccgt tttcaataaa gaaggggctg atgccgtgat
     6601 atatatccgg tttgttttca gctatggaat agccctttcc ttttgttgtg actacatgta
     6661 tcaatacagg atacccgtta tatgattttg ctctttttat tacatcttca agctttctga
     6721 tatcatggcc gtttataggg ccgaaatatt taaaacccag ttcctcaaaa ataactccgg
     6781 gaaccagcag gtatttcatc cagtctctga gcctttccgc tcctctgtac atagatcccc
     6841 ctattaccgg tatcctgtgc aggaaagctt ctatcctgcc tttcattttg aagtataggg
     6901 gctctgttct caacttgctc agatagttgg acagtccccc tatattatgg gatattgaca
     6961 ttttattgtc atttaatatc acaataagat tggtgggaaa gtctccggca tggttcaatg
     7021 cttcaaaggc catgcctcct gtaagtgcac cgtcccctat aactgcagca acggagtagt
     7081 tttctccggc cagatccctt gcttttgcca tgccaagggc agcagatatg gatgtactgc
     7141 tgtgtcctgt ttcaaatgag tcgtaaacac tctcttccct cttggggaaa ccgctgattc
     7201 cattgatttt tctaagagtt ttaaagcttt ctcttctgcc tgtaataatt ttgtggatgt
     7261 aagactgatg ccccacatcc catactattt tgtctccggg tgtgtcaaaa accctatgaa
     7321 gtgccagagt cagctcaact accccaagat ttgaagccaa atggcctccg ttttcggaaa
     7381 ctacttttat tacataatcc ctaagctcct ttgaaagaac ttcaagctcc tggtagctca
     7441 gttttttcaa atcggcagga gaatttatac tatccagaat tttatacatt tataactcaa
     7501 ccttctaact tatttttatc gcaccataat attcgcaaca acaattccca ataatgctcc
     7561 ggccataact tccttcggag tatgtcctat aagctccttc agcttttcgt cggtaatcgg
     7621 cttattgtgt gcaagatcct cgattatcat attcaatatt cttgcctggt ttcccgctgc
     7681 ccttcttact cctgcagcat catacatcac aacaaaggcg aagcataagg aaactgcaaa
     7741 aataggcgag tcccagccat acatccttcc tattgacgta gaaagcgcca ttacaaaagc
     7801 agtatgagaa ctcggcatgc ctcccgaacc tacaagcctg gtcagatcca gcttcttgct
     7861 gctgtaaaag gtaaatatta ttttgagtat ctgcgcaatc agccatgctg taatacaggc
     7921 caacagcgct ttgttgtctg ctactttcac aaagaaattc aaaaacaacc ctctcctcag
     7981 atacttttca tatgtactct attatacata ttgtaaagct tagttatccc tgtcaatgat
     8041 ataatcagac agttcaataa gatagcagct tttgtcccca tatctttcaa gctttttctt
     8101 ggcttccccg ataagctctg ccgcaatgct tatggactta tccagtccgt agaagctgat
     8161 aaaatttgat ttgtgaaatt ccgcatcctt gtttatgctt ttcccaagct tttcttcaga
     8221 acctgaaaca tcaagtatat catcttctac ctggtacgca agcccgattg catctccaaa
     8281 ttccataata ttttttatgt caccttcatc cgctcttgca ataaccgcac cggccgcaag
     8341 ggaggcttta atcagcgcac ccgtcttttt ccgatgcatg gatttcagag tctcaatatc
     8401 aatactcctg ttttcatagt acaggtctat gcactgcccg cctatcattc cggatattcc
     8461 cgaggccctg catattatat tcatagccct tatatattca ggcttggggt tatccatcaa
     8521 tatggccgtc atggtttcaa aagcactgtt caataatgca tcaccggcaa ggacagcaaa
     8581 gccctctcca aaaaccttgt ggctggtcgg ccttccccgc ctgagatcat cattatccat
     8641 agcaggcaaa tcatcatgaa taagtgaata tgtgtgaatc atttctatgc agcaggcaaa
     8701 tggaagcact tcttcctcct gccctccgaa aagctcacag gacattatgg caagtatggg
     8761 cctgagcctc ttaccccctg ccagcaggct atacctcatc gcttccataa taaccggtat
     8821 actattgtct gccggtatca tggatttaag cttctgttcc accaaatgcc ttttatttgc
     8881 cagatactcc ttaaggtcca attctatgcc tccttgattt gactgatgct gaattcctcc
     8941 atctgctcgc ttccgcggtt cataatagcc attaccttcc cctcagcttc ttccagcttc
     9001 ccggagcaaa gctttgaaag agcaatcccc tcctgataga ttttaattga ctcttcaaga
     9061 gaaatattgc ctgcttccag agtctccact atttcctcca gcctctttaa agcctgctca
     9121 aatgaaaaat tctcattcat attattcttc tccatttcaa gatattattg tagggttttt
     9181 atatatattt tatgtactct ccagtccttt gacccggact tccgcttttc cgtctttcag
     9241 ctttatataa agctcttcat taagcttcat atccttaaca gaagtcagga gcttcccgtc
     9301 ggattttctc atgattatgc cgtaacccct gcccatagct gcctcagggt tcaacagctg
     9361 cagcttttct atggtcttgt cgatttcatt ccttctgccc ttcattatat tcagcatgtt
     9421 gtaatccagg tacttgtcca gtgaatcaag agtctgcctc atattgagga ctttcctctc
     9481 caactgcctg aaaaccatat ctccggaaat cctctcaacc ctgcttctat tatcctttat
     9541 aaaatttgca attgcagagt tcagaacata ttgatactgg cttaactttc ccagcaggct
     9601 ctgcatgtcg ggaaccgcca gctccgctgc cgcagatgga gtcgctgctc ttaaatccgc
     9661 agtaaaatct gaaattgtat aatccgtttc atgccccact gccgaaatta ccggtatatc
     9721 cgattcgtat acagcttttg ccaccacttc ctcattgaaa gcccaaagct cttcaatgga
     9781 accgccgccg cggccgatta tcatgacatc cacatttccc ttggaattaa aatatctgat
     9841 cccttccgca atttcatttt ccgctcccgc tccctgcact tgcacagggt ataagtatac
     9901 gtcgatgcag gggcatcttc ttcttattat cgaaattata tcccttacag ctgctcctgt
     9961 aggtgatgta accacaccga cagcccgggg cataaaaggt attttcttct tccttgcctc
    10021 atcaaaatat cccatggcgg caagcttttt cttaagctgt tcgtaggcct tatatagaga
    10081 gcctgtccct tcgggaatca tgtcctcagt atagaattgg tactgcccgt ctctctcata
    10141 tattgaaaaa taacctctca gaataacggc acagccatcc tcaggcatga acttgagctt
    10201 tgaagcattg cctttgaaca taacacatct tattcttgag tctgcgtcct ttaaggtgaa
    10261 gtatatgtgc cccgatgagt gatgcttgaa attggatatc tctcctttca catgcaaatg
    10321 tgacaggagt acatcattct gtatcagatg ctttatataa ttattaacct cggtaaccgt
    10381 aaatattctt tcattattca tttgttccat acacctttat atccgcttct cttatgattt
    10441 tgcccagcac accgttgaca aagctcccgg attcatcagt gctgtatttc ttggcaagct
    10501 caattgcttc atttatagat acactgtcag gaatgtcgtc gcgaaacaga atttcataca
    10561 aagcaagcct taatattgcc aggtcaacct tggcaatcct ctcttttgtc catccggttg
    10621 tattgttttg tatgaattga tctatctcct gaagcttccc aatagtacct ttgattattt
    10681 cttctgcaaa cttcatgtct gcttccttca tggaatcctg ctgcccgtct tttccctgtt
    10741 ccttttttat ctcataaaaa tcctcaagca tttcatcagt attttcgttc ctgttgaaat
    10801 ccgtactata taggaattga attgcaaatt ctctggcaag ccttctgctc ataaatcctc
    10861 ctatgtattt cacaacctgc atatatttaa actaaccctc tttactgtaa tagaattata
    10921 agagggttag tttataaaag cctatttgga ttttccttca ggattctctt cttctttgct
    10981 ttccttatcc atatttattc cctgtatatg aatgtttact tccaccacag tcaggccggt
    11041 cattgtctca acagcctttt tgacgttctc ctgtatttgc caggaaacat cgggtattct
    11101 cacgccataa tcaactatta tgtataggtc aatggcagct tctttttcgc ctacttcaac
    11161 cttaacccct tttgaagggt ttttcttgcc aagtatctct gttatgctgt cagctatccc
    11221 gccgctcatg gaatagacac cctgtacttc cctggcagct atttcagcaa ttattgcaac
    11281 tacatcgtct gcaatcttaa cattaccgta cttatccaat tttatctcct tttccaaata
    11341 ttgcaccccc ttgtagattc tatgaacaat tatatcaaaa tcctaatcca tttacaaata
    11401 tatgtatctg ccaatattat attatacatt attatagtat ttattccatg ggcttcagga
    11461 taagcattac ctaagattca tagaatacgc cttagttttt cttcattatt tttatgtttt
    11521 tgggatccag ctttgttttt cttaccacaa tgtcctgtat ctgtgcaata tttgaggagg
    11581 taagctcctt ggcttcaact atgactgttg cagagtcatc ggtaagaaat accaatgcat
    11641 cgttaaaacc tctggaaatt atgagattct caattatcat ttctttatcg gtaagggata
    11701 cgattttcac aatctcctgc tgcgccatat tcttgaaatt ctcttccgcc ttgtcactgt
    11761 ttattatatc ctgccacatt tctacttcct tgcttctgtt cttatccctt tcaatcctgt
    11821 attccgagaa aaaggaagct gctgttgttt ctaatgcctc actgccgcta tcctctatga
    11881 ctattggccc cgccgcctct tctgttcccg ccgtttcagc aggcatcccc atttcatctg
    11941 ttccgattac ttgcgcgtcc aggtcaagat ttgcatcagt cagcctttcg gaagtaaaac
    12001 tgttgtaagc atacccgaca acaaaaagca gtattatcat tgaacatatt attaaatttt
    12061 ttttcttcac ttgcatgatt atacctccct gaatttatat ttaatactaa ttcctttcat
    12121 ataccgttac tctataggca ggaatatcca ataccgtttg caccgcttgg gaaattttca
    12181 gtttaacctt tgaatctttg gcgccttcgg ctactacaat tacacctttt acttccgggt
    12241 ttatctcctt tattatcaaa ggctttgaat tacctccctg agcggcagtt aatacgactt
    12301 tgtcatccgt tgttttctcg tttattatcc gtgttccgcc gcttgtatcc ttttcattgg
    12361 tcactttgtc ggaggtcgat tcatcctttg caggaattat ctctgctccg ctctctaaag
    12421 taatcattac cgaaaccttg cccgcgtcct gaatcttact gagtattgaa gacaaatccg
    12481 cctccagcct tctgacatag tcagaagcgt tgttgtctga agttcctgta tccgcctgag
    12541 taatttcact aaaaatacct tcctctgatg ccctgtccct ccggatatcc ctgtaaaaat
    12601 cggcaattaa aatcagagcc aaccccaagc agaatacgat aatcagattt gcagtggtct
    12661 tttttgaaaa gttcagcttt tgaaaattca ctttccttaa tttatccatc aaccactcat
    12721 gtaatttcat agtacttcct cccttgcatt attactgcct tacctccacc cctataatag
    12781 attcactgat tcccaaagct ttgcttatcc catccctaat ctcacttctt attctgctat
    12841 tattcaaatt atattcaact ttgtctttat taattacatt tttattaact tccgcatctt
    12901 cgtcagccgc aatttttatc ctgtcaactt ctatcacctc tttcggcctt ttgcttataa
    12961 taacctctat cgatttaatt tcaccgaaat ccttgctttc aaaatccttc tccagccgaa
    13021 gatctatcga tacttcctcg taatccgaat ccgtgctgtt gtcaacaatc cttccgacct
    13081 tctttttcag gttgttttca aaaatttcta cagccatctg acgttgaaaa ttacttattt
    13141 ccgtactgtc cgccgcggaa ccgccttgtt ctatatagct tgaagtctca agaaacttca
    13201 tattgaagct gtctgcataa tcctttatca gcacaaaggg ctttattaca actactataa
    13261 tcataagccc cacaataacg tttatataac gtttcatgga actattgggg attatggtat
    13321 ccagaaacat tataaaaatg attatcaccg ttatgtttat gatccattct cttaaaaagt
    13381 cagccataaa atcacctgcc tatactacct gaccattacc gccatatttc cgactcccat
    13441 gataaccgta agtgccatga agaacatgac agctactgct aaaacacatg tcagaagaac
    13501 cagcattgca ttgcccatat cattcaggca ttgtactatc tgattatcaa gaatcggctg
    13561 gataagcgca cttgttacct tatatatcaa tacaacggac aacatcttta taaggggaaa
    13621 actgcatata agaagcagga taatgaatcc tgcaatccct attccgttct taagtatcat
    13681 cgagcagctg acaaccgcat caaaggcttc agagaggaaa ttcccgacta tgggaataaa
    13741 gttgtccacc gcaaattttg cagtccttat ggtcgcaccg tctattgagg ctgatgcaat
    13801 accctgtacc gtcaatactc ccgtaaatgc ggtgagcacc aaacccaaag ctcctaccgc
    13861 aatttgtttc agcaatgaag caagcttgga aatattcacg gagtcagaca gattgttcaa
    13921 tatgcttata accgccgaaa aaagtattac cggcatgaca aatactttta taaatgttgt
    13981 tattagcgcg atgcatgtaa acatcaaagg ctggaacaca gccgaagaag aaatcccgcc
    14041 gacagaagcc aataatgcaa gcaatgttgg cagcagagcc tgcatgaagc ctgtcatttt
    14101 attaatggta tcaactccga taagcattgc atcattaaaa ctctttacag ccagaataat
    14161 tatcagtatg tagcacatcc cgtatgccaa ccggccgatg gtatcctcct cgaaagcatt
    14221 atggaggttt tgaagtatgg cacaaaaaat agccagtatc agaagcttgc ccataaggct
    14281 gaaattgctg attacttctt tgaaaaacaa gcttgcaata ccttttccca tatctctgat
    14341 gctgttttga gtatttccct taaaaaggcc caataccatc tcccttacat ctatctcggg
    14401 cagcatgcct tcagattccc tgttgatatc atcaacaaac ttttctatat cgcttatatc
    14461 cagattatcc agctggcttt gcagaatatc ctgtgtatcg tcgcagttag cggcacataa
    14521 ggaaagcaga atgattatca taaaaaatac tatgcctttt cccatgtata tcccccttaa
    14581 ggtatgattt ttatcattat atccatcagt gccgcaagta ttggtatcgc aataaccata
    14641 ataagcactt tccccgcaaa ttcaaccttt gcggcaatgg caccctctcc ggcatccctg
    14701 catatctggg cgccgaattc cgttatatat gctattgcaa taaccttaag tattgtagtg
    14761 aagtataata cgtcaatatt ggctttagcc gcaaaatgct tcagcaccgt catgacggag
    14821 ttaagcttgg ttattacaaa tacaaatata accaatccgg tgacaatgct tattgcaaat
    14881 gccatttcag gcttttcctc cttcaggact actgcaagca atgccgcaat tatgcctacc
    14941 ataacgattt tcatgatatc cataatatca ccctacaaat ggaacattgt tcttacacta
    15001 ttaaaaagat tgctaataag gcttattacc atcataagca ctactacaac tcccgccaaa
    15061 gtagtcatca tagccatttc ttcccttcct gactggttca ggactctgtt cagaaccgct
    15121 gtaactatac ctatagccgc tattttgaat atcaaatcaa catccatttt atcgcccctt
    15181 tcgccggtat ttatgccaat actacaaata ctgcaattcc caataaaaaa ccgagacttc
    15241 tgtacatttt tgcacttctc tttatttcct ccgttgctgc agcctcttga tggctgagct
    15301 gaagataaaa aagctggaaa tgctttttct ggtcttccct gtcggagcat cccaatatgc
    15361 ttccaaagct ctttataaga tcaatgtccg ccattttcaa tgaagactga gtaagaatcg
    15421 attcagccga atcgttccat gcttctctca cagtataaaa gcttctgtcc gaaaggcacc
    15481 gggagataca gctaaaaaag ctgccaatcg ggccttccgc cttttttgat acgctgaaaa
    15541 gtgcggcagg caagggtgta gccccatatg tcacttctgt ttccagcatc tgcattaatc
    15601 tcctgagatt tgcaagctct ctgtatcgca atgacaattt atatgaataa accatcccta
    15661 ataatccgct tgcggaaatc accagcaggc ctccgataat cttaagcatc aactctatcc
    15721 ccccatttac aaatgctttt gcttctttca tacagtacat tgaattcccc atccaagaca
    15781 gtttcaagag tgccgacacc cttcttcctg ctcaggacaa tatacctttc aaaaactccc
    15841 ttttgaatac gtttaagatc ctgcttattc aaaaaatcgg ttatattgct gccgtgtatt
    15901 gtagttatga ctttcacccc cgcattgact gcagcggtta tggcatctgc atcctccgcc
    15961 cttcctattt catcggcagc cactatctca ggagacattg accttatgac cataataatt
    16021 ccttccgcct ttgggcaccc atccagtaca tctgttctta tccctacgtc atttctgggt
    16081 atgcccatac tgcatgcggc tatttcggag cgctcatcca ccaaagaaat cttcatgccc
    16141 ttaaagggta tgtctctgct tccgctgctg agctttctga ctaaatctct cagcaatgta
    16201 gtcttgccgc attgaggcgg tgatataata agagtgctga atatgctgtc gggaggctgt
    16261 accaaatagc ccgatacttt gtctgatgcg cctaatacct gttttgccac tctgaaattc
    16321 atatagctta tgtccttcat ggtctttacc ttgccgctgt caactactgc ccggccgcat
    16381 acccccaccc tgtggccgcc gcttaccgtt ataaaaccat ttctcagttc ttcctccacc
    16441 gagtacaatg aaaaattgca tgtcagctga agtgcacgct tcaagtcttc ctcgcttaca
    16501 agatatgcct cttctgcctc ggatactgcg tacccgtccg cagaaataaa aagctcccca
    16561 ttgctccctg ccacgcataa gggctttgcc agcctcaatc ttatttcttc cgcatccata
    16621 agtatttttt ccgggagctt cgctatcatc gcccctatat gcttcggcat atattccagt
    16681 acctcaagta tttttatatc attcttcata aagcatctgt cctttgtaca aattttaatt
    16741 tgtactatag atatgacaac tgatttgaaa taattactgc gggaaaaaaa ataacccgat
    16801 tgaatacttg gattcaatcg ggttataatt cagcttcttt tgccggctat tcctggtcat
    16861 ggtcgcagcc gcagccgcac tcgtattcaa attcgatcgg ctcatgacag ttagggcata
    16921 tgacctgctc accgctttct acaaggtctt cgtcaagata gacaaattcc ttgcaatgag
    16981 gacactcaac ctcaatgtaa tcatagtcct cctcgtcatc aaactcttct tcgtcatctt
    17041 cgtcatagca caagtcatcc tctaatgctg aaaggtcttc atctattgct tcaacatatt
    17101 catccagctc attttgtgat tcattcagtt catttactgc atctgcaaaa tcacttaata
    17161 catctataat tttagtgatt aacttgccct gatctgttga ctcgtctacc ttcatccctt
    17221 ccgccaggcc tttaagatag gctactcttt cgtataggta ttccatatgt actcctcctt
    17281 attttttata tgctctcagt attcttacca gtatcggcaa ctatatacag gattatactc
    17341 tttccatgta ttcccctgtt cttgtatcaa ttctgattct gtcaccagta ttgataaaca
    17401 aaggaacata gactgttgca cccgtttcta cgatagccgg cttagttgct ccggtagcgg
    17461 tatcccctct gaaacccggt tcagtctgag ttacttccat ttccacaaag ttctccggct
    17521 ctactgaaaa agcctcccct ttataaaatt ttatattggc tgtatcattg ggcttcagat
    17581 acataattgc atcctctacc tgctcatggt taagaggtat ctgctcatag gtttcattat
    17641 ccataaaata ataaagtccg ccgtcttcat acaaatactg catgcttttt ctctcaatat
    17701 gcgcctttgg atacttatcc gtaggattaa atgtcctttc agttactcct ccggttatga
    17761 tattttttat ctttgttctt acaaaagcag cacccttgcc gggcttaaca tgttgaaagt
    17821 ctacaattgt atatatttgc ccatccattt caaaagtaag accttttcta aattcactcg
    17881 ctgatatcat taatcatttc cctccgtgta tgtatttatt taaactgaag actcctactt
    17941 ctgcgaataa tataatatta acataatttt tccatagcgt acaggttttc ctttgacaat
    18001 ctgccgtaaa ttttttttat cttcaatgca tcctccgcca tagtacccct tgactcgctg
    18061 ataattgttc cggaaatccc aaatttgata aggcactctg caaggggctc gaattcaggg
    18121 ccatactcaa catcgtcata ggaccagtgc tttttctctc cgcccttttc cgtatactct
    18181 accctgctgt aatgcatgtg catcaatctg cctctctttt ccccaagaac agtgaaaatc
    18241 ctgtcaaata ccttttcata atcctccctt ttcctgagtg cgcctgaacc aagcgcatga
    18301 agatgtgcaa aatcaacagt cggtatcagt aattcatcca gcaggcaaag ttccagtatc
    18361 tcatccaatg tccccatttg attcctcttc cccagggtct cgggacaaag ggaaaccttg
    18421 attcctttct tatttattat ttccaatact ctgaataaat tactccggat gttttcaaaa
    18481 gccttattcc ttgtcagctt acccaaagag ccgggatgga gaacgacacg catagctccc
    18541 atcaactctg ctgtgattga ggtatcaata atataattga tggatttctt aacagcttcg
    18601 ggctcctctg atgcaagatt tatatagtac ggagcatgta cgctcaaagt gattccgttt
    18661 tctcttgctg cctttcccaa ccgttccgcc atatcaggct ttattctcac acctcttgcg
    18721 caggagtact cataagcgtc caatcccata tccctaagcc aggcaggcat gtcaagggag
    18781 cttttatagc cttgctcata aaaagaatct gaattacccg atggtccgaa atgtatcatt
    18841 ttgcgctcct taagcaattt atttaaatgc ccttgctgcc atatataata gtatatccaa
    18901 ttttctcata catccatgct gcttcagaat agcacctgca cttattcctc attgtgcctg
    18961 ggattctcat tcacaagaaa cgcagctttt attattgcag tcccaacatc tatatcaaac
    19021 tgctgttcca attcgctgac ctttgcatac agctcccctt tagcagatac aacgtacttg
    19081 gacggaaggc tgttcaaagc aagtgctatt atatccagct tgcatttatc acacttgcag
    19141 acatctatgg actttaaaat ttcatccgtt cgcttgatta ccaaatcctc catatagttt
    19201 tttaatttat acatacccag tcctccttat taattatatc taaaagaacc cgagatatat
    19261 gcccattatg cttttcccga aaacaaacgc ggtcagagcg ccaatagaaa ggaaagggga
    19321 aaatggtatt gtatcctttc tcttctttat cccgaacaat atcagcagca gacttgccaa
    19381 cgctcccgct gcaaatgcaa ttacaagtga aagcatcgaa agcttcaatc ccagtattgc
    19441 accgtaaaca gcattaagct ttatgtcagc tcctcccatt ccgccttttg tcaatatggc
    19501 tatcaataag agtgttccgc cccctgccat cagccccaaa aatgcatccg ccggctgaaa
    19561 gccctttatt aaaaggccca ctagtccagc gcccaaagcc gcaatatgaa tttcatcctc
    19621 tatctccctg tattttatgt cctgataaga agaaaccaca gctgtcagaa gcagaacatt
    19681 cagtatcaaa tagcttcctt cggcattctt atttatcata tacaaattta cagataaaaa
    19741 cagtataaat acaggtgccg caaagcttaa gcctattttg tcgcctttgc ccgccccgac
    19801 cttcctctcc aaaagcttta cgagcaaaac agaagcagca gtaaaagcgc tacttgatat
    19861 aacagtcaaa ccgtgcataa tcaataaccc ttcctggctt tccatccgcc tggcaaacct
    19921 ttagtcctaa aaaaaggctt caccaataat gaagccttta ccaatttatt tcaacttata
    19981 tcattctaca aacaacaata aaaatcctgc aaatactcac aggattttat ttcatttatc
    20041 attgcatatt atttgtttga cgatatttcc tcaaaacatg cttttccagc ttccggacaa
    20101 ttcttgtcca tatgaattcc ttattactta ttggtgacac aagtatggtg aacagcccca
    20161 agcggttacc tcccagtaca tccgtaaaaa gctgatcccc tattactgcc gtatcagcag
    20221 gtttggattt cataagcttc atagctcttc taaaggcaaa cttgcttggc ttggttgctt
    20281 tatgaattgc aggaaggttc aggcatttat tgaattcctc cactcttttt cttgaattat
    20341 ttgataatat acagatttta aagccttccg aacccaatcg gcacataaat tcattcgcct
    20401 tttcgtcagg ctctcttgta ttccacgaaa ccaaagtatt gtcaatatcc actataattg
    20461 catttattcc atgttccttc aatgaagctg tatccaattc atataccgaa ttcaaataaa
    20521 tatccggttt aagcctgttt ttcatatctt acctcaattg tacatttttt atgaatgctg
    20581 catgtataca gcagccttaa gaccccgtac ctctattcta ttcttacccg ttttcattgt
    20641 aaagctcttt atgcagcttt ttgattgccc tattttcgat tcttgacaca tatgaccttg
    20701 aaattccaag catatcggct atttccctct gagtcttgct gattccgtta agcagcccat
    20761 acctcagttc aataaccagc ctttctcttt ttttcaaagc tgtatccatt ctttgataca
    20821 gtttcttaat ctgaatctta agctccactt catcaagtat tatatcggaa tcacttccca
    20881 atatgtctat taaacttatc tcgttgcctt ccttgtcaat tcctatcgga tcatggagag
    20941 acacttcggt ttttgttttt tttgaggctc ttatagtcat caggatctca ttttcaatac
    21001 atcttgcagc ataggtcgca agccttgtcc ccttgctctc atcaaaagtt attattgctt
    21061 taatgaggcc tatggttcct atggatatca aatcatcaat ttctcttccc gtatttacat
    21121 actttttcac tatgtgcgcc acaagcctga ggttccgctc taccagtatg ttttttgcat
    21181 cttcattacc tcttttgtac tcttcaagat aatactgctc ttcttcagtt gtaaggggct
    21241 gcggaaatga gttcacattc tgaatgtaac cgaataacag gcataggtcc tttattgtga
    21301 aaacaataag agaggctaaa aagagcagca aagaagcaca cctccgaatt ttaaagaagt
    21361 ctgccacgta caaggttcat acaatggaaa acttcgccta ttgaaattat atgatttaca
    21421 ttccgaatgt gtgcctgtat aaatacataa cataaaaaat tttatgtgaa tctatcgttt
    21481 aactgcagtc aaaattttgg aagctgcctc cttaaacaac ggagctgccg caccgccccc
    21541 cgattgcccg ttatatatga ataccgttat ggcatacttt ggcttttcat agggaataaa
    21601 gcctgcaaac cagccgtgaa caacctctcc ttctttaatt cccgtctgag ctgagctggt
    21661 cttgccgcag cagcctccgt attcatccat atttgcctgc tttcccgtac ctgttaccgt
    21721 gacttcatgg agcatatcct ttaacagctt agaggttatg ggattaagca caatctgggg
    21781 ccttgacctt tcaagctttt tgactgtcaa gcccttttcc gtaataaggg aatctattat
    21841 ctgagggtca ttcctgatgc cgttatttgc tatggcagcc atcatacatg ttacctgaag
    21901 gggtgtaacc tgtatcttcc cctgtccgat ggatatattt cctataccgt cctcaaaagc
    21961 ttccggtatg taacctgcct tttcttccgg aatggtaaag cacagttttt gcccgaggcc
    22021 cagccttccg gccattttca gtatgttttc agcccctgtg gctgtaccga tctgtataaa
    22081 agtgctgttg caggacaggg caaaagcctc cttcatatta atctctatgt cttcatgggt
    22141 tttatgggtg gagcatttta tcctgctgct gcccacatct gcgtagcccc ggcatttgta
    22201 tttttcttcc gggttcacat accctccttc aatggcagct gcagccacca cggttttaaa
    22261 tattgaacca aggtcgaatt gccaaattga tttattaatc agctcgtttc cgctgctttt
    22321 caggtatttg tccacgttgc cctgatcaaa atcaggtttg cttgccattc ccagtatatc
    22381 gccatttttt acgtccatta ccactatgga gccttcaacg tcgttttttt ccattgcggt
    22441 ttcaattata ttctgaatat gatagtcaag ggtaagttta aggctgtagc ttacaccgtc
    22501 tcctgccgcc tcaacctttc tgaagcccag cccccgtatg atattctttc cgctgtccac
    22561 tacagctgcg atactgtcgc tgcctctgct ttccaggtaa ctgttcatac tcttttcaat
    22621 acccatttgg cctgttttgt ccgtttttcc tatgtagccc actacatgcc ttgcgatatt
    22681 gtcatcagcg tatctcagcc ttttttccac ggcaatgaca ccgcgtatcc tgccgctttc
    22741 aatcaatgac aaatactcat tctgctctgt ttcacatatg aattccgccg tatctgccga
    22801 acccatcttt ttttcaacat cttccatttt aatgccgcat gctttggaaa tggtctcaac
    22861 agcggttttc ttatcatata cgtaatccgg atatgcaata accacttttt ttacttctct
    22921 atcggtaata ggtatcatat tcctgtcata aataataccc ctgtttgagg aaatgggtat
    22981 atgaaccatc ctttgccgca cagccctctc actgtagtgt ttacccatat atacctggat
    23041 gtagaaaagc ctgcacagga gaagcccgca tgcagcaata aataaaagac ctattacctg
    23101 aagtcttttc ttatgaataa gaatatagtt gtttttcctg taaagccttg tacctggcag
    23161 tgtaagaagc tccatactcc tgttttcctc ttccttcctg cactccggca cagctttttt
    23221 tcttaagaac ttcataattc cggccataac aaaacctccc tcaatcttag tattgtcaga
    23281 ttgtgggagg ttattcactt cagtttgaat cacagccgcc gtcaataatc gatttaatct
    23341 ttgtcaccat tatatctatt gccaccttat tgtagccccc ttcggggata attatgtctg
    23401 cgtatctttt tgtaggctca cagaactcat tgtgggcagg ctttacaaca ttcaggtatt
    23461 gggtaatcac tgactcaaga gatctgcccc tttccctcat atctcttaca attcttctta
    23521 taaccctgac atcagcatcc gtatctacaa aaatcttaat atccattaac tctctgagcc
    23581 tcggagaatc cagaataaga atgccttcca gaagtattac atctttgggt tcaactctca
    23641 atgtatgctt tttcctgttg tgcacggtaa aatcatactg aggcttatca atagcttgtc
    23701 cgcttatcaa ctttaacata tgagcaatca gcaactcgtt atcaaaggca aggggatgat
    23761 catagttcac gttaactcgt tcctccggcg gaagatggct ttggtccttg taatagcaat
    23821 cctgttcaat gataactact tttttccccg gtatgctgtc cgtaatagcc tttgaaaccg
    23881 tgctcttgcc tgacccggtt cctcctgcta ttccgattac aagcggcttt ctatgctttt
    23941 cttcactcac cgtctttcct ctcccttctc aatatatcat gtttttccac gggcctctcc
    24001 attttgatgg ttacaatctg ctgcgggtgc ggagcagata atatttcaac tccctcatcg
    24061 ttccacatct tctcaatttt ttgagtaaac atctctctgc ggggccctat tacctcaatt
    24121 tcatctccca ccagcatttt attcctctgc tctaccgttg cgatgcctgt cttgctgtca
    24181 taatccaata ccaaacctac aaaggcataa tccctcacat aagcgccgga ttcataaatc
    24241 tgcccgcgtg aatcgggctt gccgaaaaaa aagcctgtgc cgaattccct gtggctggcc
    24301 tttgacagct cctccagcca ttctttcctg aattcatagt tcccgggatt cgaaaaatag
    24361 ctgtccaggg ccattctgta tgctcttact acagatgcca cataatatga gcttttcata
    24421 cgcccctcaa ttttcaggct ttttactccc gattccgcca attcaggaat aaattctatc
    24481 atgcataaat ctcttgaatt gaatatgtaa gttcctcttt cgtcttcgtc aaccggcata
    24541 tactgtccgg ggcgtttctc ttccacaaga tagtacttat acctgcaagg ctgtgcacat
    24601 tcgcctctgt ttgcatctct gcccgtcata taattgctga gcaaacatct gcctgagtat
    24661 gaaatgcaca ttgctccgtg tacaaatact tcatattcca agctttttgg agacttttct
    24721 atagtttccc ttatctcttc aaagctcaat tcccttgcca gaactattct ttttattccc
    24781 tggctgtgcc agaaccttgc gctcatataa ttcgtattac tggcttgagt gcttaaatgc
    24841 agctgcatat caggcaatac ttccctggct gtcattatta cactggggtc agatacgata
    24901 agtgcatcgg ctcccgcatc tcccaggctt gttaaatatt cctccagccc ttccaaatct
    24961 tcattatgcg gaataatgtt tactgtaaca tatacttttc tgcagttatc atgggcaaac
    25021 gcagcacctt ctttgatttc atcaagggag aaatttcccg cagcggctct gaggccatat
    25081 ttttcaccgc cgatataaac cgcatccgca ccgtaaataa tcgccatttt taatttttcc
    25141 aggtttcccg ccggagcaag taattccggt ttattcaatg aactcaacct ccttaagctt
    25201 gcagcatacg gatataccgt cacccatggg aataacagca gtctccaggc tttcacagga
    25261 agtaataaag ctcagaaact tcttgagcct ttttacaata gtgattttcc ttcttatcag
    25321 caagcttctt tcagccacca ttcccctgaa aagcacgttg tcacatacat acaggccgcc
    25381 aatcctgagc ttgccgatgc tttttttcat aaaatccata tactgtcctt tcgccgcatc
    25441 aacaaatatc atatcaaagc tgcctggaat gtctttcagc atttcttccg cagctccggt
    25501 tattatcctg atattatccc ccatgccggc cttcctgata aaatctgctg ccaatgcagc
    25561 atattcttca tttctttcaa ttgtagttat tgagaaatct ttaccggcac acagccccat
    25621 atgtattgcg gaatatccta tagccgtacc gatttccagt atggatttca cctttgcagt
    25681 ctttataagc actgccagca agcttgcaat attgggagta ataatgggca catggttctc
    25741 ccttgcatac tcttccattt cctttagtat tccgcttcgc ccgggtatca tgccaagcag
    25801 gaagctgtcg atataatcat ggctgatatt tgccacagta tcacctcgca taaaaaaaat
    25861 aatgcgtaat atataggttt gtaaagtact ccgactgtag gggaagtatg ttctcctcac
    25921 cttccaaagt cagcaaaatc actggcgcgg gtcccatctc tgcgggcccg tctggttatt
    25981 ttgccagggg tattcccgtt ccgtggtcag gtggaaggtt tcgtcgaata tagcttcccc
    26041 acgtcggtat ttgcaattct cattatgtcg cattattctt ctatagtttt tgaattactt
    26101 tctatattta ttctgagtat tgatatgctc gttgtatgtt ttcgtgaata catgggaacc
    26161 gtcttccaat gcaaagaaat acaagaaatc cgtattttca gggtaaagag cggcttctat
    26221 tgccgcaagg cccggagatg caatgggtcc gggaggcaag cctgcatact tgtatgtatt
    26281 gtagggtgaa tcaatctcca agtcctcata cagcagttct tctttcggtt cctttaacag
    26341 atactgtacg gtggcgcagc tttgaagcag cattttcttt ttcagcctgt tgtggaatac
    26401 tcctgatata gtctttcttt ccgaatccag cttagcttcc ctttctatta tagacgccag
    26461 ggttaccacc tgatcaatgc tcatattaag ttcttccgcc ctgcttctga atttctccgt
    26521 aaatatatcg tcgaagcgtc ccagaagctt tttgataatg gcctcagcgg tagtatccct
    26581 gtatatttca taggtatcgg gaaatagata gccttccagt ttattttccc tgtcagggat
    26641 ctgttctatg aactcatatt catatccctc cgctttcaaa gcagcctgta tgctctcagg
    26701 acttacaacc ccgagactgc taatcctttc agctatctga gcaagattgt agccttctgg
    26761 tattgtgaac tttacggtat ctataagtgc cttcccggat accagtacat caactatctc
    26821 cgaaaccttc attgaactgc tgatgctgta tctgccagcc ttgattttat ctgctttttc
    26881 attaatctta caatacaact caaaaattaa gctgtccttt atcaaatcct cttctttcag
    26941 aatttttgat atatatctga ttgtactgcc ttttggtatg acaatttctt taaaatcaga
    27001 tccttctccg cttactgcct ccattccgtt attgtaatat acataagcga gaacaagggc
    27061 aagaagagaa cagaaaacta aaaacctgaa tatgttaaat ttgagtttcg ctttcattat
    27121 aaacacttct ttcaaaaatc gctgacttta ttatataatg tgtaacatct tttgacaaga
    27181 ttaacagaat aaaaatgcac ccaaagaagg acggggacat ggggacgttt ctcctgtccc
    27241 gctttcagcg ggacaggaga aacgtcccca tgtccccgtc tttgtgtcct attttttact
    27301 tattcctggc ttttgccctg aggctgctgt ccaatatcct cttccgaagc cttattgatt
    27361 ttggagttac ttccacaagc tcatccgccg caatgaattc aaggcattcc tccaggctca
    27421 tctccttatg gggagtcagc ctcagtgctt catccgaccc ggaagccctc atatttgaca
    27481 catgcttctt cttgcagaca tttacctcta tatcatcgga ttttgcattc tctccgacta
    27541 tcattccttc atatacctgc actcccggtc caaggaacaa atatcccctt tcctgagcgt
    27601 tatagagtcc gtaagccgta gtctcgccgt tttcaaaggc aataagggag cccctgggcc
    27661 tgtcgggaat ttcgcccttg tattcctcgt agccgtagaa aacatggtgc atggttccgt
    27721 ttccctttgt atcggtcata aactgggacc tgtagccaat aagccccctt gccggtatct
    27781 tgaattcaag gcgcagataa ccgtcgctgc tgttgcccat attcatcatc tcagccttcc
    27841 tggggcctaa tctgtccatt actactccca tgaattcttc gggtatttca ataataagat
    27901 gctctatggg ctcacaggct gcaccgttga tattcctcat aatgacattc ggctttgata
    27961 cctggaactc atagccctgc cttctcattg tctctatcag tattgaaagg tgcagttctc
    28021 ctcttcccgc cacctcgaaa gtgtcggggg aatccgtttc cgtgagcctc attgccacat
    28081 ttgtttccaa ttccttaaac aatcttgccc ttaaatgcct tgaagtgaca taatcccctt
    28141 cgcggcctgc aaaaggactg ttatttacgc taaaggtcat agaaattgtt ggttcatcaa
    28201 tattgacaaa gggtattgcc tccggattct gaatatcgca aattgtctct ccgatattta
    28261 tatcgtctat gcctgaaacg gcaattatgt ctcccagaaa tgcctcttca acttccttcc
    28321 tcttgagccc catgaaactg taaatcctgc tcagctttac actttgcagc actccgtccc
    28381 ttttgcaaag agcagcgctc attccggaga ttatcctgcc tctttcgatc ctgccgatag
    28441 caatcctgcc gacatattca tcatattcta tggtagtgac aagcatctga aaagggcctt
    28501 ccgcatcacc tttgggaggt tcgacatagg atattattgt atcaaaaagg ggcctcaaat
    28561 cctgagactc atcctttata tccagctttg ccactccctg ctttgcagaa acgtatacaa
    28621 tcgggaattc cagctgatta tcatccgcgc caagttcaat gaacaaattg ataatttcgt
    28681 caatcacttc ctcaatcctt gaatccggcc tgtctatctt gtttattacc acaataggct
    28741 tcagttgaag ctccagagcc ttcttcagta caaaacgcgt ctgcggcatc ggcccctcag
    28801 cggcatccac aatgagcagc acactgttta ccattttcag gaccctttcc acctctccgc
    28861 cgaaatctgc gtgccccgga gtatcaacta tgtttatctt gatattgtta tatattactg
    28921 cagtattttt tgccagtatg gttatgcccc gctctctttc caggtcattt gaatccatta
    28981 ctctttccat gacgttttca tttgccctga aagtaccgct ctgccttaaa agcccgtcca
    29041 ccaaggtagt cttgccatga tccacatgtg ctattattgc tacatttctt atatcttctc
    29101 ttatcgtctt ttccattgta tcttctccaa tatataaatt aggttaaata aggattttca
    29161 atgccttggg cattagctca aaaacagcgt cagccctgcc ggggatttct ccatccgctt
    29221 caataaggcc tttgccgccg cttgttacct tgactttgct gcctctgcaa tacttaagct
    29281 ttgggtggtt catatgcttc cctttatata tcaagggaaa gctttttata atatcatact
    29341 tgcttaagtt tcccagaatg attatgtcaa atactccgtc gtccaacacc gcatccgggg
    29401 ccaccatcat tccgccgccg aaaaacttcc cgtttgccac tattacactg ttcataattc
    29461 cgttcaaatg tatcttatca tctataacaa cttcaaaatt ttgattcttg tacaaaacca
    29521 cagtgcttaa tgttccaagc ataaaggaaa gaaagcccga aagcctcttt gaatgcatat
    29581 ttactctgtt tgctgtctcg gcgcctatgc ccgtgtccgc gacattcaaa aaataccttg
    29641 caggcatatt cccttcagtt cggacaaggc ttaaacgtcc cacatcgatt tgtttttccc
    29701 gattcctttt cagaatagcc aataaatcct ctatatttct attaattcca aggctccgta
    29761 caaagtcaca gcccgttcct cttgaaaata ctacaagcct tgaattctca tcaatgagcc
    29821 ggtcattttc aaaaaaaccg ttgaccactt cattcattgt gccatccccg ccgacagaca
    29881 taattgtatt atagccaagc tttaatgcct tccttgtcag ttcggcagca tgtcccgggt
    29941 attcagtcag tacagcttca aattcaaaac ctgcatccga caataccttt tcaaataagg
    30001 gccattccct ggctgttgtt ttgtttgctg aaacaggatt aactactata catattttat
    30061 ccataacagt ctaaatctcc ataataattg gaagaatcat gggatttctc ttggttcttt
    30121 catatatgaa ctcccggagg ctatccctga cctcggattt gatggttccc cattctgtta
    30181 tctgtttttc ttcgcatttt gccagtgcat cctttattac tccccttgcc tcttccatta
    30241 agtcttcaga ctcacggaca tatacaaagc ctcttgatat tatatcgggg cctgccacta
    30301 cctgtcctgt ctccttgctt atggttacca ccacaactat cagcccgtcc tgggaaagat
    30361 gctttctgtc cctcagaact atgtttccca catctccaac tccaaggccg tctacaagta
    30421 ccttcccggc aacaacggtt cccgctgtcc tgccgctgtc tttagtaaat tccagaactg
    30481 ttccgatatc ggcaataaaa atattctcct tgttcacacc cattttttcc gccaattggg
    30541 cgtgctgcgt aagatgcctg tattcaccat gcaccgggac aaaatacttt ggttttatca
    30601 gggcttgaag cagtttcagc tcttcctggt atgcatggcc tgaaacatga atatccacca
    30661 gagactcata tataacatct gcacccttct taaagagttg atttataact ctggatacaa
    30721 gcttttcatt tccgggtatc ggatttgctg aaataacaac tctgtctccc ggtattattg
    30781 ttaccttccg gtgttctgca gtggacatcc ttgccagggc tgacaaaggc tctccctggc
    30841 tccctgtggt aagtatcatt attctgtcat tcggatactt gtctatatgg tcaacttcta
    30901 tcataactcc ctcggggata ttaatatagc ccaactctgt agctactcct attacattga
    30961 ccatgctcct gccgacaacc gccaccttcc tgttgaacat tgcagccgca tttattgcct
    31021 gctgtatcct gtgaatgttt gatgcaaagg tagccaatat tactcttccg cctgcatctc
    31081 tgaaaagttc catgaaggta gctcccacag tgctttcaga cattgtatat cccgggcgct
    31141 caacgtttgt gctgtcaccc ataagcacca gcactccttg ttttccaagt tccgcaaatc
    31201 tgtgcaaatc aatcagctca ccgtctatcg gctgataatc gattttgaaa tcccccgagt
    31261 gaactataat gccaaccggg gtatgaattg ctagggcaac tgaatcagct atactgtggc
    31321 ttgtttttat aaactctacc tccagttcgc cgaatttcac catgtccttt ggcttgacaa
    31381 ttatccgctc aaccaatgaa agaagtccga attccttaag cttattctcc accagtccca
    31441 gggtaagctt tgtaccatat acgggaacat ttatctgagg aagcacatag ggcaaagcac
    31501 ctatatgatc ctcatgtccg tgagtaagca gtatggcttt gaccttttcc ttgtttttca
    31561 ccagatatgt tacatcaggt attacaagat caattccaag catctcctca tccggaaaca
    31621 tcagcccaca gtcgattatg actatggtat ccttgtactc gacggctgtc atgttttttc
    31681 cgatctcatt cagccctccg aggggtatta attttaattt tgccttactt tttgccactt
    31741 tctttatcaa tcctttcatt ttttccgttc ttaatatatt tcacactaaa catactatat
    31801 gttccgtgcc aaacatctta ctttaggcta ttagctatta ttagtcaata gaatatcatt
    31861 tattactatc agaatatgcg ggcagggctt cctgtacaat taaaatggta ttgcatatgc
    31921 tatgcaatac cgcaagtcac tttctacact tttcacagta tccgaaaaac ttaacgttat
    31981 gattacttat cttaaaatta tatttctttt ctataccgga ctccaattgt tccagcaaat
    32041 cctcctcaac ctctattacc ttgctgcacc cgacacatat gagatgatga tgctggtggt
    32101 actcattgtg gtgattaatc tcatacctgc ttcttacctc atcgaaatta tgtttatata
    32161 caagccctac ctcttcaaac atctgcatgg ttctatacac tgtagccagg cctatatcgg
    32221 ggcactttac cttaaccagg tcatagattt cttccgcgct taagtgcttg ccttcagtct
    32281 ccaatatgat gtcaagtata gctcttcttt gaggggtcag tttaaaccct ttctccttaa
    32341 gcttttccct taagtactca aactctcgta tcaaagcttt caacttcctt tatatcttgt
    32401 ttaaaaagtc acatcttgca cacctatatt ttgatatatt agtatttttt tgtacaatat
    32461 tccttacatt taatattatt atttattctg catttttgtc aactaatttt tgtttattgg
    32521 cttttgccgc ctctgcaaag cacttcaggc aattgcccgg gcatgacagg ctgcactgcg
    32581 caacagttcc gcatctgggg catttactct gctccttcaa tgtatatccg cacttgccgc
    32641 attttaaacc attcttcata tcaaccatgc aactatccct cccgtgatga atgctgccgc
    32701 aaagcttccc agccatattg ccagagcatc ccttgtgcct ctttccttaa aaataaccat
    32761 tattgctgct atgcacggca caaacagcgt cataactatc agagaaacaa gcagctgctg
    32821 ctgtgtcatt gctattgaag tgagccctgc ggcaccgaaa tccctcctga taatgcccat
    32881 aataaatgcc gttgccgttt caggcggaag ctttagcaag ccttccgtta ttggtgtcat
    32941 ccactgctgt atatatgaca atgcacctgt ttgctccaaa acacttataa gcaatgcccc
    33001 gtatatgaac agcggtactg cctccttcag gaacatcacc gtcttctgaa aagtcttctt
    33061 cacaacattt ccgggcatcg gcagcctgat tgtgggaagg tctataagaa gatgggagct
    33121 ttctccgggc atcagcttat tcagcaccgt accggcaagc acaaatattg aagtaataac
    33181 tgtgatatac gtgattatat agaatacatc caatcctgaa accagcccca ttattacccc
    33241 aagctgtgcg gagcatggta tcgcaagccc caaaagtacc gtagcgataa tcttttccct
    33301 ctttgtaccc aatatccttg tagtcatggt tgccattgta acacaaccaa agccaagtat
    33361 aataggtata attgccttac cgttaagtcc gagtgatata agcatcctgt ccgccagtgc
    33421 agcaattctc ggcaggtaac cgctgtcctc cagaactgcc attaagacat agaaaccaat
    33481 gataaggggc aataacaggc ctattatata tacaggcacc attgtaagta ttccgaattc
    33541 tccgatcagc atattgccta taaagctgcc ttcactgata aaaagtgaca gtactgatat
    33601 aataaagggc ttgtacatgc cttccatgat taattcctcg gtaaagccta cgaccgttcc
    33661 cgctacgaaa atcccaatta ttttatacat ggcatataaa gtcagtaaaa gcatcggaat
    33721 tccggtaaca ggcctcagca tccacctgcc cagcagaatt ccgaaggaag ctcccttact
    33781 tgtttccttc agtactttat ttactatttc ttcaaccgcc ctgcgccttt ccatataaat
    33841 ttcatcacgc aagccggagg tttcgatgcc gtatctggca gcgacaacct cgtcatcctc
    33901 aagtatcatt aaagcttccg cctcgctgca tgtatccccg aactgcttca tcatttctgt
    33961 aagcccggga tctctattcc cttgccttgc ctgttccaat gacatcaaca cgtccgttac
    34021 tccaatattt ttaacagcag aagttggaaa aaccgggact cccagcagtt cttccagcag
    34081 cttaacatca atatccatgc cgttcttttg caattcgtca atcatattca acgccactaa
    34141 taccggtttt cccatatcta tcagctgttt tgtaaggaac aagtccctgc ccggatgaac
    34201 actgtcaacc acattcagta taatatcagc ttcaagtatt acatccctgg caaccgtttc
    34261 ttcttcatta aaggaagaaa ctccataaac ccccggtgtg tccgctaccg tgtagccctt
    34321 gtatttgcct gtgcttatat caaccgttgt ccccgggaaa tttgagacat caacgtatat
    34381 tccggtcaaa tgattgaaaa aaaccgattt gcctgaattc ggattgccgg caagaaccag
    34441 ctttttatcc tttatatctt taatgcaatt tttattgcaa ttattacaac cactcatact
    34501 accttatccc ttcaagtttt tacatttata ttttttctac ttctatgccc tctgccagtc
    34561 ttctgcctat ggcaacctcc tgcatcctgc tccgcagtat tattggccct cccgcaagcc
    34621 tctcgctgca aagaaagtct gccccttcat ataaaccaat ccttattgcc tgagttttca
    34681 tatccctatt atttatcttg gtaattctaa ccttatttcc tctcgcaacc ttatccagtg
    34741 tcatattcaa catctccttc tatttgatat tgattctcat tcccattgat ttaataatag
    34801 cattactgat attgattgtc aatatcattt agaaaataaa aatataaaac aaaaccccca
    34861 ggaaaaaatc ctgcgggttc gttgctttgc ataagacggg caagccttga aagagcttac
    34921 gggtaattgc tgtaaatatt atataggaat accgccgctt cggcccgtgt ggcatatgca
    34981 cagggattca gcttatttcc ggtgcctgcg attaatcctt ccgtgataag agcagacata
    35041 ctgtttacgg catactcggc gatgtcttcc ttgtcggcga atttctccgg cacaccgttg
    35101 gtatccaaaa ccttcaatcc tttgaatctt tccagtgctc tggctgtcat caccatcata
    35161 tcctgtcggg aaatgctttc ccgcggattg aaccggttat ttccgctgcc ggcgacaatg
    35221 cccagctttt ttgcaatgcc aatggcatcg taatagtagg cactcttatc tacatcatca
    35281 aaattgctgt caaattccgc tgcaagcccc agtgtcttga ccagcagtac cagataatcg
    35341 gccctggtga tgctggctgc gggtgagaag gtatcattcc ctgttccatt gatgataccc
    35401 ttggaagcca ttacctcaac gggttttctt gcccattccg cactgcagag gtcactgaag
    35461 gtcttatgta catatacaat cgcataatga ctgaaatgcg tagtgttaaa ggtcactgtg
    35521 ccggtggccg ggtcgtagcg cccgttgggt acggaaaccg catttccgct tccgtcaatg
    35581 taccatacta caatgctttc gtggttagca agctctgccg cagccggggt ataagggatg
    35641 cagacagtaa ccggagtgtt tgggttactc cagtctgtct gctttccgtc gatggagacc
    35701 gttaaccgga taatcggacg gtcgccgacg gcggccttca ccgcgtcagg caggatattt
    35761 ttatctccct gaccgatgga aatttgcgcc ttgcttccgc taatccccgg tatacccgtt
    35821 agcatattgg acggaatagt gatgctgccc ttatctgtgt ttacggtcag cgtgccctgt
    35881 gcatcagccg ttgacaggct cgtcatcggt atgctgactg aataagcctc aatgtcggag
    35941 acggtgggta tggtgattac cgatccgtct atatcaaagc tttgtaagcc tgcgtccacg
    36001 gacgcagttc cgccgttttt gtcaacggtg acagtaagca cagcttcctc gccgtctttg
    36061 ttctttacct ccgccttgta ttccggttcc gccgcttttg gtgcgttttt attgcctctg
    36121 tcgtctctgt cgccgctgct gcgtgtaaaa gtggctgtaa cggttacggc gtttgcgggt
    36181 actgtaaatg tggtgctggc ggaagcagcg ttaccaaagc tgccgccgct gccggtaatc
    36241 catttatcga actgatagcc gctgtcaggc gcggcggata tttcgataac cgtacctgcc
    36301 gcatagctgc cgctggtgcc gttggtgatg cttccgcctg tgcctgcagt gaatgtcaca
    36361 ggataatgcg caatgatggt cagggtaaag tcttttgtct gcgccgtacc gccgtccctg
    36421 gtgatggcag ccgtcagagt gacggccttg ttaccgtcat cggggaatgt aacttttccg
    36481 tcagaaatgt ctatgcagcc gaccggatcg gcgctccatg tgattgccgt ggtgccgctg
    36541 gttccggtat caggaagttc aagatcagcc gtcacatgat cctccgtgct gtttgtacct
    36601 ttgatgacag cccatgtcag ggcagcctta tccagcgtga tggcttcatc cggcgtcagc
    36661 ggcttgatgg tcaggctgaa aggctttgtc tgcggtattc cgctgccttt gctgacggta
    36721 gccgtcaggg tgacgattac ctcgtcggtg tagggctttg atagtgtgcc gttgctggta
    36781 ttgatatagc ccgtgggatc ggcactccat gagattaccg tgtcgcgccc gcccgatata
    36841 ataagcacaa gagccgaggt tacgtcattt tccgcgctgt ttgttccttg tatatcggtc
    36901 caggcaaggg cggcgacggc cgcggtgatt tcctcgtcgg ctgtccatgt ctgccattgt
    36961 agagtgggat aggatatgtt ctgaccgatt ttccagatgg tgttgaaatt ccagccctca
    37021 aagctgtccc attgcttcat ttgcacagcg gtcaaagggt cgccgcctgc tgaggtgcct
    37081 gcggtatcga ggtaatagct gtcgttgacg gtggcgttgc cgccgccaat cagaccgccc
    37141 aaagaggccc ccgttccgcc ggatgcactg ccggtgctgt agctgttggt gacggttccg
    37201 tcgtcgacgt cgccaatcag gccgccaact cttgcattgt ccccaccggt gatggttccc
    37261 gtgctatagt tgtcggaggt actgacatag cttcttccga gcagtccgcc gacattggcg
    37321 ttattgccgc cggtgacgtt ggcggcgctg tagttatatg tgataacgcc gccgccgttg
    37381 ccaatcaagc cgcccacatt tgcgctatcg cctccggaga cactgccctc gctgctgctg
    37441 tttgtgacgg ttccctgatt cacgcccgtc acaccgccta cataggctcc ccatgtgatt
    37501 gaactaatat ttacgcctgt gagcctgata ttttccagag ttcctcccgt accgacagat
    37561 ccgaacagac cgacatagtt ttgcccgtcg tcgttgatgg tcaggttagc tatggtatac
    37621 ccattgccgt tgtatgctcc ggtaaattga tccacgttat ccccgatggg cgtccagttg
    37681 atgcttgtca ggtcaatatt cgccgtctgg atgtagtgtg ctcccaagcc ctttatgcgg
    37741 atttcgtcaa ggtgcgccgc ggaagatacc tgataaggac tgtctgcatc gccgctgccc
    37801 acccatgtaa cgggcacatt tttgacggtc agggtaaaat cctttgtctg cggcgtaccg
    37861 ccgtccgcat ggctgatggc ggcggtcagc gtgaccgtct gatcgccctg cgagtacagg
    37921 ggcgttgtga cctcgccggt ttcggtgttg ataaagcctg cgggagcggc gctccatgaa
    37981 atgctcgcgc cctgcgcgcc cgatgcatta agcacaaggt ctgcggttac gtcgctttcc
    38041 agggtgttcg cgcccctgat agcatcccaa tcaagagcgt catgggctgc ggtgattttc
    38101 tcgtccgtcg tccacggctg ccagcgcagg gtggggctgg atacgccctc gccgatcttc
    38161 cagtcggtat caaagtccca gtccacaaag ctctcccgct gtttcagcgc cgtcgctgat
    38221 aaagccgtcc ctcttgcgga ggaagctgcc gctgtatcaa ggtaatatga gcttgtgacg
    38281 gtgggattgt ctctaactaa gccgataaag cctccgacgc tgccatcgcc aggtgttacg
    38341 tcgcccgtgc tgtagcagta tgcaatagtt gcgctggtac tttgaataag cccaataaag
    38401 cctccctgat ttgtatcaga gccgctgttt aacagcgata cgctgccggt gctgtagctg
    38461 ttggcaatgc ctccgcttcc ataaaaatag cccaccagtc cgcctatacc atagttacta
    38521 gcattatttg ttccatttac gttacctgta ctgcagctat tagtaatagt tccgttgttg
    38581 acagtaccga ccagcccgcc gattgttcca tcagatggcg ttccagtcac attgccggtg
    38641 ctgtaaccgt gttcaatggt tcccgaaccc agcaaataac cgaccagccc gcctacataa
    38701 tatgttccag tcacattgcc ggtactatag ctgtttctga tactccctcc ctgttgacga
    38761 cccaccagtc cgcctatagt attgctgaca ccttcgccgc cggttacggc gcaggaactg
    38821 ctgctattct caacggttcc acctaaataa ctatcgccaa tcaatccgcc tgcgcgggcg
    38881 ctatctccgg tagccgttat actgccggaa acgcagctat ccttaatcgt tcctccgcaa
    38941 ttttggccgg tcagtccacc gacgaaggca ctatctctgg aagaattgat gctgacattc
    39001 tcaagaataa tattttccag cgttccgccg ctgcccgcat agccgaacag gccaacataa
    39061 tatagctcag tgttgttgat ggtcagattg gatatggttt tgttattgcc atcataggtg
    39121 ccggtgaatt gagtggtact gtcaaaactg ctgttatccc cgatgggtgt ccagttgatg
    39181 cctgtcaggt tgatgtccgc tgtctggatg taatgagcgc ccattccctg attgggaata
    39241 tccgcaaggt gctccgccga cgatacctga taagggctgt tttcatcgcc gatgcccacc
    39301 cattccacgg cagacatcgg gctgaacagc atagacagtt tttcctcttt gagttcggtt
    39361 acgccgtaca cgctgtccgt caccgaaaat ccgattttcg gagcagcttt gtcagccgcg
    39421 ccgccgtcac cgctgtcaag cccctccgcg taagctgtta tcgggaagcc cgtcatgccg
    39481 acgatcatgc agagcgtcag gaacagagct gttgttttcc gaaacaagtg tttcattgtt
    39541 ctcgtcctcc aatctatgaa attttgcgac cctgatttta accgtagggt cgtagtgtcc
    39601 tatgacaaat cccattgtat atggtttttg caaatcaagc attgacactt ttaagctcgg
    39661 agagaaaaaa tcaggttttt ttcaaaaaaa ttaacccgcc gtttggcggg ttaggtgtcg
    39721 ggcatgttca ttcggttatt ctatgtgatc tccgtcagcc tccgccgaca gaagccggac
    39781 ggcatgtccg agaggcatgt cgctgtattg cgtttgccgg gcgagggccg cgctgtccgc
    39841 acggtggagc ttctggaagc ccgatggcgt ggcgtggaag aagcgccgga aatacttgat
    39901 gaggtatttg acgtcggaga agccgcagtc cagcgcgatc tccacaatgc gcatgtcggt
    39961 gccgagcagc agccttgccg cgcgctcaca ccggctgtag cacaacagct cctgaaaggg
    40021 cactctgaac ttttccttaa tgtcgctgct caaatgctgg aggctcacac ccagctcggc
    40081 ggcaagtacc gtcaagccct ttttcacctc cgagtcgctg gttgcattct gcgccatctg
    40141 tcgcagcctt gccacacgct tttcgtcaaa gggctcggta ccaaagcccc aacgcaggaa
    40201 gtcaaagcta tatgcagtat agctcagcat ttccatcaga agcttccgaa gggcttcctg
    40261 accgatccga ttcggacttg aaccaagcat accgaccagg cgggcgatat attccttcag
    40321 gacaccatac ttttcaggtg cctgggcttc gtggtaagga gaacagcagt agatgaacat
    40381 gtagtggttc ggcagtacgc tgcggcagaa gccgctgtcg atatggagga aaaggatttc
    40441 atcttctccg tcacccgtca tgcagtgaat ctcgtttatg ttcgtgacgg caatatcgcc
    40501 ttccctcagc accaggtcat cgtcacccat accgatatcg accgagcctt tgagaacctg
    40561 aataatctcc agtgcgtcat gccagtggta agggtactgg ttaccgctgc gaacaaacgc
    40621 cctgaccggt attccgtcat taaattctat caattcagga cgcatgtccg tcaccacctc
    40681 acaggcatct caggattatt ttttcgacat tttccttttt ttcattataa ttccttaaca
    40741 ggaaaattac aaggtgcttg gcaaactgac tgaatacaat aaaaagcaaa aaagccttag
    40801 ggatcgaatc cctaagacta cggaacgcat cgatgaactg caagcctcct ttaatttgat
    40861 tcttctctca ccagttcctc atatgcttct attacggtat taagttcttc ttcatcatct
    40921 atacctatga gtatgtcttc tccattttcg tcctgttcta ttctcaatac atatgcctcg
    40981 tccacttcct cagcatcggg gtctcttgta tcgtctctcg gtagcagtat agcatattca
    41041 ttttcatcca cttccaaagt agcgaccact tcaaattcgg tctcattgtc attttcatcg
    41101 taaagaagaa ttatgtccat ttcatcactc attcaaaaat cactccaatc tttctcgtaa
    41161 aactatttta tactactgac aataatgtgt caataaaacc ttaatatttc aaggattatc
    41221 atttttcata ttgtccaggt aacattgcaa tatcaaaact gcggccagct tatctatgac
    41281 tttctttctg tccttccttc ttacatcagc ttcaagcaga gttctttcgg cagcgcaggt
    41341 agtaagcctt tcatcccaca tctccacttc aatgtccgga aaattctcct ccagtattct
    41401 tacaaactct atggtttttt ctcctcttgg cccgatagtg ttattcatat ttttgggcaa
    41461 tccgactata attcttttta tatctttctc acaaataatg ttttttaaac aatcaatgtc
    41521 ataatatatg ctctttcttc ttattgtggt tatgccatga gcaatcatcc caagctcgtc
    41581 actgacagcg acaccgatag tggcatcccc caaatccaat cccataattc tcataaaaat
    41641 tctcctccat atatttttac acaaagcacg ggtaacacaa cacctgtaag gaatacccgg
    41701 aagttccccg cgacctcgca acctcgtaac ctttcatacc accttaatgc aaaaatcagg
    41761 gcaataagcg ctgcaatatc cgcaaagcac acattttgaa gaatcgacca ctgccttgcc
    41821 cttttccaca ctaatagctt tctgtgcgca tttcccgctg cagcttccgc agccgctgca
    41881 ccagtaatca atcagcagcc ttcttttttt cttctcaagc ttcagcctga tatcttccgg
    41941 cacctgtttt ccttcaaaaa tcattacgtt gcttgctacc tcttcataac tctgcatccc
    42001 aacggctatt gaatgcaggt tgtcgtttga aagtacaaag tcaaagcatt ggctgctgtc
    42061 ctttatcata tttcctccgc ctaaaggctt cattgaatat atgccttttc ctgccttgta
    42121 tgcctcagct gctgcttcaa gcattccgtt tacgtctccg tcaattatcc ccaggcccct
    42181 tttattcaat atcggatgaa gcacctctat atccttgtat tttaatgaag cccttaccgc
    42241 ggcaatggca tgggtagata tgccaaagct cctgatatac cccttttcct ttgccctgac
    42301 gaaatattca atggcttcct gatgcccttt taatgtatgc tcgctttcct gctcatgaag
    42361 cgaaaaaata tctatataat ccgtatttaa ttcatccagg gcttttctaa ggcttttttc
    42421 agcaccttcc cgggtatagg catagcactt tgttgatatt attatatcct ttcttcttcc
    42481 ctctgcagct tttcttatat aatcatatgt tctgtaaagc tccgctgtgt caatgaagtt
    42541 tactcccata tcaaaggcgg cccttatcac cgatgcccct tcttcaatat caagccccgc
    42601 ttgaagaggc ccgatggtga ggcttccgaa acagagcctc gatactctca gccctgtgct
    42661 tcccaaaatc ctgtaatcca tcttcagacc gccttccctt cccctatgca cataatcaac
    42721 atggtaaatg ccataaatat catcgccgtt gcggctacta caatgcctga atcatttact
    42781 gcgaagcctg cggcggctga agcggcaata ctcatccatg agtatttcag atatttatac
    42841 ctgttgaata tatagaacat ctgttttaca ggcttgtaaa acattatgga tattgcccct
    42901 attatgcaca gcagcacttt tgtccatatg gtatatctca tcagcctgag attcattgat
    42961 attttcctgc ttatcacaga ggtaataact ccaacgccat tggcacgggt gtcctttata
    43021 agggctccca tgtgggattg cctggccaat cccaggctgt ccgcaataaa caatgccgct
    43081 gcagccgacc ccaaaatcag tgctcccgta aaaatatttc ttctgttaaa ttttatatca
    43141 aaaaccagga aataagcaag caaataacca aaaacccctg caactgcccc tccaaaatta
    43201 gcgcccagag acgtcaatcc aagaaggagc gtgcaaaaag aaaaataagc agctgcggca
    43261 gccttgccgc atctccgtcc cctcagctcc gaaacacagc caaagaccat aagggaaata
    43321 ccgatgaaca tccctgcgta ttcatttccg atgccgtaaa accttgcgcc gattatggga
    43381 tcatacccca gcacagactt ttttatcagt tcgccgcctg taagtatatc agcggcaagt
    43441 cctgcctgca gcagcaggca gattgccagt ataaccttca gcctgttcct taaggtcagg
    43501 tgaagcaata ttgacaatac tgcagaagcg gcggcagtca gcacccaata ttcggcggat
    43561 ccccaatccg tataagatgc ggatattaaa agaaaagcga ttggaatcga aagcattgta
    43621 tatgcaataa taatcccaaa accagaccaa aaatgccccg ctttctttcc gagaaaaaca
    43681 gccgcgccaa acagaatccc gagtactgca atcacagaca ccgcataata agtaagaaca
    43741 ggagttctca gaacggaagt ccttaaaata ctcatattca tatacgttaa agcatttaac
    43801 gaagcttttt tatccaattc ctcgacggca cattcagtca ggtatcccag cttacagagc
    43861 acataatccg caatgtccgt attcaaaatt atgccgctcc gccttgtatt gtttgagtaa
    43921 agcactcctt cccttccttc ataaaccaca atgggagtca gtttattgcc cgcttccgca
    43981 tttgatttcg aagggtaggt tgatatgaat acaagtgttt taaaaccccc gtaactgaca
    44041 atcttcttta caaatccgtc aatcctttcc agcacatcct gtttgcatga ttggcaggct
    44101 tcctcggaca tggagccttt aaaggcttcc agcctttcca tatcccctgt ctctattaca
    44161 ataaatgaag aagccggcag atattgcttg tacaattccg ccagcctgtc ataatcagtc
    44221 ctttttcctc ccgggaaagc agcatcttct ataatgacat cctctgtctc tcctgaatca
    44281 atttcaccgc tgctgtccgc agcaattagc atagaacttt tgttcggctt ctctgcatct
    44341 gcatttccga tatagcaggc agtcccgccc ttcttattaa ttgcatcgcc tatgtatccg
    44401 atgtatttct tatattcact gtcggcattt ctgtctttga tcctgttaat acccgtatat
    44461 acgagactac ccgtcaaaga ttcccttctg ccttccgaaa ccatatcggc atccaactca
    44521 agttttttcc cggatccgat tatgagtttt gatttggctg catcggcttt ccccggctgc
    44581 ctgttgttta tgagggcaac acggctgttg ccggccagtc tgtaaagact ctccatcctg
    44641 ccgatatcct tgatatccag gtaatctccg actatcatca ctaccttgcc gtattcctct
    44701 tctccggatg caaaaatttc ataagccttt acatcctgca aagcaataat tgctgcactt
    44761 aacgcagcaa tggaaaaaaa agcgattaag cttttgttca aactaatctc tcctgaaccg
    44821 tatttcagct acttcccatc gacataagcc cttacaagct cttcaaggag ttcgtctctt
    44881 tcaagctttt taatcaggct tcttgcattg ttgtggcttg taatataggt agggtctccc
    44941 gaaagtatat atcccactat ctgattgatg gggttgtagc ctttctcctt aagtgcgctg
    45001 tatacagaca gaagtatatc cgatgcttca tacttcttat ccgtctcaac cttgaatttc
    45061 attgtgtctt cgaatctttc gcccattatt acacccccta ggttcacata tatatatata
    45121 ataatataga taataattca tttcaaggaa atgtttgcat gtgtaaccgt aatgtcatgt
    45181 aaggatgacc tctgctgtat gttccgctcc gtcttcggca agcggaatct catttccttc
    45241 aagcttcctt ccgtccagga tgatttctgc ctttgcattg ccgctcctgt tctgatttct
    45301 tactattata ttatatatcg tattcttgtt gtgtctgtac ctaatattgt actcaggcca
    45361 atcccttggt atacatggtt ccatcacaat cctgcctgct tttttcctga atcccagtat
    45421 atgctgcatt cctacattat acatccagcc cgatgcacct gtgtaccagg tccacccccc
    45481 tcttcccgtg tggggaggaa cggcgtacac atctgcagcc attacgtatg gctcaacttt
    45541 gtaccttgag gcttccatca tagtatttgc atgattgatg gggttcagca tattaaacag
    45601 cttccaggcc atatcaggat ttttcattat tgaataagcc ataatcaccc aagttgccgc
    45661 atgggtatat tggcctccgt tttcccttac accgggggga tagcctttta tataccccgg
    45721 ctccagatct ccttcatcaa agggaggtgt aagcagcttg acaatccctt catcatactt
    45781 tacaaggtac tgttctactg cctgcatggc ttcccttgcc ctgtcaagat ttgcagctcc
    45841 cgatatcact gaccaggact gtgcaatgga atcgattttg cattctgtat tttccgccga
    45901 gccaagggcc gttccattat caaaatatgc ccttctgtac caatttccgt cccatgcatt
    45961 ttcctcctgc gcctttgcaa tttctttcgc tatgtttatg tatcggtctg ctttgttcaa
    46021 atcccttctc atcctgcaca gctccgagaa attcataagt atgtcataca ggaaccaacc
    46081 cagccatacg ctttcaccct tgcccttgtt gcctaccgtg ctcataccgt cattccaatc
    46141 tcccgatccc attaagggta taccgtgatg cccgaatttc aacgatcttt caattgccct
    46201 tacgcagtgg tcatacagtg aagcactgac atctgataca caaggtatct tgtatctttc
    46261 atcctctcct tccctcagcg gttcatcctc aaggaattca accctttcct caagtatttg
    46321 cacatctcct gtcctgttaa tataatttac tgttacatag ggaagccata aaaggtcatc
    46381 cgagaatctt gtccgtatac ctttattgga gccgggatgc caccaatgct gcacatcccc
    46441 ttcaataaac tgatgggaag cgtgcagcag aatctgcttt cttgcaatat caggcagtac
    46501 atcaattact gccattgcat cctgaagctg gtctctgaag ccaaaagctc ctcccgactg
    46561 atagaatgcc gttctcgccc atattctgca gcttaatgtc tgataaagca gccatccgtt
    46621 cagcagtata ttcatatttt catcgggagt caaaaccgtc acttttctta aaatatcttc
    46681 ccagaaagct tttgcatcat ccaaagcctt atataccctc ttcacatttc tgtacctttc
    46741 actgtatgcg ctcacagctt caaggctttc cccctggccc agcatgaata ccacttctgt
    46801 ctgttcccgg ggcttcagct ctataaccgt ttgaaggcac atacaggggt caaagcctgc
    46861 cccaagtgta tttgaaagct tatccttccg cagcgcttcc ggatgcttaa gccctcctat
    46921 attgcccaaa aactcccttc tgtctcccgt gtaatacctt tcctttgcat tagtatccag
    46981 aaatactatc cttcccggga aatcggtatt atagttgttt ctggcaagaa tggcgccgtt
    47041 gctgtgcaat tcggtgtaaa cgtgctgaac cgtaccttgt tcatatacac ccaatacagt
    47101 tcttgcataa tatatagccg acagcttcat ttttgtatta gagccgttcc tgagcttcaa
    47161 tatgcacagc tttacagtgt cggagaccgg aacaaattca gtcagttcat gctcgattcc
    47221 gttccagttg tgctcgaaaa ccgaatagcc gaagccgtgc cttacaatat aaggagtttt
    47281 cgtccttatg ggatgagggg taaccgacca tatttcacct gtttcttcat ccctcatata
    47341 tattatctcc cctgagggat caatgacagg atcattggac caggtggtca gcctgttctc
    47401 tctgctgttt tccgcccagg ttgtggaggc accggattca gtaatcagaa aaccgaattt
    47461 ttgattggat attacattgc accagggtac gggagtattc acgccttcag taagcttgat
    47521 tacatattcc ttgccctctt tgtcaaaccc tccgaaaccg ttgaagttct taatttcaag
    47581 ctttttcata tcgtacttgt tatgcttttt ccctttgctc ttccatatct tgtactcaaa
    47641 tgcgttttta ccgtttctca cttttgtctg taccgaaagg agtccgtatt tacccttcaa
    47701 tactatcctt gccgctgccg caataagctg cttatcctct tccggcatgg agtacccctg
    47761 ctttattgtc actcctcctg ccttatccct catatctctt gcatggctta ccgaaataat
    47821 atcctg
//
LOCUS       DAOOAB010000016        46286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_989_length_46286_cov_14.786139, whole genome shotgun sequence.
ACCESSION   DAOOAB010000016 DAOOAB010000000
VERSION     DAOOAB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 46286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46286
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_989_length_46286_cov_14.786139"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..3175)
                     /locus_tag="PKU88_05235"
     CDS             complement(359..3175)
                     /locus_tag="PKU88_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX68723.1"
                     /translation="MDKGAHFYRCDFQVHSPRDINWHGPEAISNEERKAYAETLVQAC
                     RTKGLHAIAITDHHDFVFFPYIKQAALAELNDNGVCYPDEEKLIVFPGLELTLASPPC
                     QALLILDSAFPENLLQGVLTTLGITPNPDTESRTATIVPIPQAVTKNFCELYEKLSNL
                     DYIKGRFTIIPHVSDNGHKTLLRSGFADFYKTMPCVGGYLESSISTLGRGNTDIVNGK
                     NRHYGFKEIALIQTSDNRKATHEDLGRHSTWIKWAEPTAEALRQACLAKNSRISQEIP
                     VLPPVFITSLDVSNSKFLGRIYLEFNRQYNAIIGGRGTGKSTVLEYLRWGLCDQYPDL
                     SQEDEELPNYQERRKKLIERTLLPYDGTVQVSFIKNGIVHVVRRKAKSGEILLKIGNE
                     DFEVCTEENIRNLLPIQAYSQKQLSSVGVSGTELKRLVYSPIRQNLSEYDLHFKNLNT
                     DIRACFEKRNQINLLQKEIHRRELELKSVTEQVENLRKNLKGISDEDRATIDTHEAYS
                     ETEQLVEEWVSELTAASNSLDVLLTEIEAFPTPIPPRLIIPDASKDTIKTINDSISQL
                     FKDVKKQLNDIKKNIAEYSAAENEFSHGRTDWMQLLLIHKQHYESAKERSSSQQATLT
                     QISQLEKRSNEIRKSLGEKKQLLNKLGDQDQKFSRLKEQWVSSHNGRADTIQAQCEQL
                     SILSDNNLRATLGRGKGIDSLGSTLRSILAGSNIRKEKTDSICSFVSNATNPINEWQE
                     LLNEFESLANFDFENDSPETFPSTPKLVSIGLSQNDLRKIAEKMNINNFINMYLTQLE
                     DIPVFEYKTKEGEYIEFTDASAGQQATSLIHVLLNQEGPTLIIDQPEDDLDNQMVSEI
                     AELICKSKKNRQLIFTSHNANIVVNGDAELVACCDYRVSGDQSNGEIKHLGAIDISII
                     RDVITKIMEGGEKAFKLRKDKYGF"
     gene            complement(3688..5808)
                     /locus_tag="PKU88_05240"
     CDS             complement(3688..5808)
                     /locus_tag="PKU88_05240"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPX68724.1"
                     /translation="MDVFELELAGRKLSIETGRIAQLADAAVLVRYGDTVVLVTACAS
                     AEPKEGIDFFPLSVDYEERLYAVGKIPGGFIKREGRPTEKAILSARLIDRPIRPLFPH
                     GYRNDVQVVATVLSVDQDNLPEIAAMIGSSAALSISTIPFQGPTGSVLVGRVDGELVL
                     NPNNEEREKSDLHLVVSGTKDAVMMVEAGAKEVPEDVMLEAIMFAHDNIKKIVAFIEE
                     IVAKVGKEKKEYTVFKPEEEIETAVREYATQKTLDAIRTPDKLERQEKIDAVKEETFE
                     HFAEIFPEKEKDIDEVLYDILKEQVRKLITEEGIRPDDRKLDEIRPISSEVGVLPRVH
                     GSGLFTRGQTQVLTIATLGALGDVQILDGLTEEESKRYMHHYNFPPYSVGETRFMRGP
                     GRREIGHGALAERALEPMIPSEEEFPYTIRLVSEVVSSNGSTSQGSVCGSTLALMDAG
                     VPIKAPVAGVAMGLMKEKGNVAILTDIQGMEDFLGDMDFKVAGTSKGITAIQMDIKIA
                     GIDRPILERALEQARVGRLFILDKMLSVISEPRKELSPYAPKIITTRVDPDKIRDIIG
                     PGGKTINKIIAETGVKIDIEDDGRVFIAAVNQAAGDKALKIIESLVKEVKEGEIYLGR
                     VTRLMTFGAFVEILPGKEGLVHISKLDTKRVEKVEDVVAVGDEILVKVTEIDKQGRIN
                     LSRRHAMADVAKEEEKAEEEKEKA"
     gene            complement(5964..6233)
                     /gene="rpsO"
                     /locus_tag="PKU88_05245"
     CDS             complement(5964..6233)
                     /gene="rpsO"
                     /locus_tag="PKU88_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033016867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPX68725.1"
                     /translation="MALTKESKGELIQKYQIKEGDTGSPEVQIAILTERINNLNEHLQ
                     INKKDHHSRRGLLKMVGQRRGLLNYLKAKDIERYRAIIEKLGLRK"
     gene            complement(6384..7301)
                     /locus_tag="PKU88_05250"
     CDS             complement(6384..7301)
                     /locus_tag="PKU88_05250"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPX68726.1"
                     /translation="MKIAEQYDEAMKSAPWCIALGSFDGVHIGHRKLIEILKEESRLH
                     GTGSMVYTFHTHPRKILIPNKHIYLITDNKKRSEVIEQMGVDMLYLEDFNKIREMRAE
                     DFVKDILVSRFSVKSVVIGYNYKFGIKGEGNVDDLKQYGEKYGFRVIIVDAFEVNGHI
                     VSSSLIRHVIRSGNVDKVSQYLGRDFSVHGEVVYGKQNGELMGFKTANIDVKKDITLP
                     KKGVYYTSTKVCGRLFKSITNIGFNPTFNGESLSVETHLLDFCDDIYGEEIEIFFHKH
                     KRDEMKFDSIEGLVEQINRDIQDRYTFES"
     gene            complement(7439..8350)
                     /gene="truB"
                     /locus_tag="PKU88_05255"
     CDS             complement(7439..8350)
                     /gene="truB"
                     /locus_tag="PKU88_05255"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPX68727.1"
                     /translation="MNGIINVLKPPGMTSHDVVSYLRKVLNIKKVGHTGTLDPEAAGV
                     LPVCVGKATKAVQYLTDKQKSYRANIKFGTVTDTFDSYGQVIKEYESVVIDKKALEDT
                     LKSFTGAISQKPPVYSAIKIKGKKLYQYALEGKEIRIAERPVEIHELKLVEMISEDEA
                     IIDILCSKGTYIRSLCHDIGEALGCGAHMSQLIRLGSSPFSIEDSYTLEEIKAAAEEN
                     RINDILINVEILFKHYKSITVKASALKSITNGNPLYEQGVLNGFEGLNENEDIRIYGE
                     AGFIGTGTVQNDESGQRLFIKIKNIFI"
     gene            complement(8360..9313)
                     /locus_tag="PKU88_05260"
     CDS             complement(8360..9313)
                     /locus_tag="PKU88_05260"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPX68728.1"
                     /translation="MIFQNIISKIKESSKIAILSHIMPDGDSIGSSLALYNALKKCGK
                     DVRFILDDEIPKIYAFLEGAEQVRKPGQFENFDAVIALDCGDAERLGKSSLYLNDNYV
                     INIDHHISNKEFGSLNLVDTGSAASGEIIYELIKSLDIGMDREICECLYTAIVTDTGQ
                     FQYSNTTSVTHQIAGDLIDNGVSVPLMFERIYQNNSKEKVMLMKTALSTLEFFHNDAA
                     SCISLSLEQMKEANALEEDTDGIINLARDIGCVEVAMFLKEIEPGIIKVGLRSKKKAD
                     VAAVALRFGGGGHVHAAGCTVHGTIAEVKEKILEAVAEELR"
     gene            complement(9310..9675)
                     /gene="rbfA"
                     /locus_tag="PKU88_05265"
     CDS             complement(9310..9675)
                     /gene="rbfA"
                     /locus_tag="PKU88_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPX68729.1"
                     /translation="MANHRIDRISEEVKRVVSDIIRNDLNDPRISTMCSVVSAEVTAD
                     LRYAKVFVSILGSDEEKKATMKGLTNAAGFIRRELGRKIELRYLPEVIFELDRSIEHG
                     AYINKLLNEVKKKDGGENL"
     gene            complement(9708..>11867)
                     /gene="infB"
                     /locus_tag="PKU88_05270"
     CDS             complement(9708..>11867)
                     /gene="infB"
                     /locus_tag="PKU88_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPX68730.1"
                     /translation="QPPQAQKPQQKPQTVQQPQQRPQSAQQPVRPEQKKDIRPAGKPQ
                     QHPSGDKRNPSSDIRPVSAPKNVSAHDEIEEDAITLSTKKDFTKVERIGKKLPGKSPQ
                     GKPPFRQQQQGKPYNKNFKQRGFEAPKKEEVHVSKKPVKIGDMISLKELSERLGKPVT
                     EIIKKLLLLGIIANINQMLDFDTAQLICEEFGFTVDKVIDKESEEVLLKTEEDRPEDL
                     RPRPPVVTIMGHVDHGKTSLLDAIRQTNVIATEAGGITQHIGAYTVTIGDRKIAFLDT
                     PGHEAFTAMRARGAKVTDIAVLVVAADDGVMPQTVEAINHAKAADVALIIAINKIDKP
                     GANPDRVKQELTEYGLVSEEWGGDTIMVPVSAKKREGISELLEMILLVAEMQELKANP
                     NKKGKGTVIEAELDKGKGPVATVLIQDGSLSLGDYFIVGSTHGKVRGMIDDKGKRIKK
                     AGPSTPVEIQGLDEVPDAGDIFIVVDDEKTAKTISEKRKEKQRLSQIQSKQTVSLEEL
                     FNQIQEGKVKELNIILKADVQGSVEAVRQSLARLSNEEVKVNTIHGGVGAITESDVML
                     ASASNAIIIGFNVRPQPMAIALAEKEKVDIRLYRVIYNAIEDVEAAMKGMLEPEYKEV
                     LLGHAEVRATFKASAVGTIAGCYVTDGKINRNNDIRVIRDGIVIHEGKLASLKRFKDD
                     AKEVNAGYECGLNIEKFNDIKEGDIIESYTTEAVPRK"
     assembly_gap    11868..11967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11968..12299)
                     /locus_tag="PKU88_05275"
     CDS             complement(<11968..12299)
                     /locus_tag="PKU88_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HPX68731.1"
                     /translation="MNKIRIYELAKELGVQSKELIAAAKELNINVNNHMSSLEDSEAS
                     AIKKHLKKDDNVEAPKPIQAQQPKKRTLPQKPQQPQQPQQTQQKPQAAQQPQQEQKAQ
                     RAPQTAQQP"
     gene            complement(12292..12633)
                     /locus_tag="PKU88_05280"
     CDS             complement(12292..12633)
                     /locus_tag="PKU88_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPX68732.1"
                     /translation="MNKAYSMISLANKAGKLVTGEDAVRNSIRNGRVKLVIISEDASD
                     NTKKRMSNSAEFYSIPFIVWGLKDEFGISLGKSARSVLGITDESFSNGIKAKLIDEVR
                     KKEKPGGGFIE"
     gene            complement(12626..12895)
                     /locus_tag="PKU88_05285"
     CDS             complement(12626..12895)
                     /locus_tag="PKU88_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPX68733.1"
                     /translation="MQRKRKVPLRKCLGCNEMKPKRELIRIVRSPEGKVDIDKVGKAP
                     GRGCYICPDLTCLEAAIKAKRVENALEAPVDEDVFNRLRTQLSHE"
     gene            complement(12910..13950)
                     /gene="nusA"
                     /locus_tag="PKU88_05290"
     CDS             complement(12910..13950)
                     /gene="nusA"
                     /locus_tag="PKU88_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPX68734.1"
                     /translation="MNGEFLQALEQIAKEKGISKELLIDAIDVALVSAFKKNFGTSQN
                     VKVQINRETGEVDVYALKTVVEEVDNEFMQISLQDAKAIDKNYEAGNVVETKVTPKNF
                     GRIAAQTAKQVVVQRIREAERGIIFDEYISRENEIVTGLVQRKEKNSVFIDLGKTEAI
                     LGPGEQTPNEVYNIGDRIKTYIVEVKKTTKGPQIILSRTHPGLVKRLFELEVPEIQSG
                     IVEIKSIAREAGSRTKIAVYSHDENIDPVGACVGQRGHRVQVIVDELKGEKIDIIQWS
                     NDPEIFVGSALSPAKVIEVFADKETNAARVVVGDSQLSLAIGKEGQNARLAAKLTGWK
                     IDIKSQSQAYGI"
     gene            complement(13975..14436)
                     /gene="rimP"
                     /locus_tag="PKU88_05295"
     CDS             complement(13975..14436)
                     /gene="rimP"
                     /locus_tag="PKU88_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPX68735.1"
                     /translation="MAKRKVEDIVYELAKPIIDRNNFELVEIEYKKEGPYWYLRVYID
                     KEGGITIDDCQSVSEELSDLLDIADPIEQSYIFEVSSPGLDRPLKTDRDYRKNIGKPI
                     EIKLFYPLEGKKIIEGILKGHTESSVEIEVEGKALNVEKASIALIRPLIKF"
     gene            complement(14627..18901)
                     /locus_tag="PKU88_05300"
     CDS             complement(14627..18901)
                     /locus_tag="PKU88_05300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HPX68736.1"
                     /translation="MQNDMKPDSNLFLQYKDKFELKKIRVLKKAREWEMIISLKGFIP
                     IPELGEIESAIAEYFKLNKVRIIIDESSVSLSADELVKSYWEDLKKAVIASNPSFTGF
                     IMDSNVQLENNVIRITVKSEEAAKYLTAKKCNDIIERYINDYFNISLHVSICADNEEK
                     YCEDEYLKCYFEEENKIITEALKPIKSDNSGKAKENKNELIMGKPFKDTPVSIASITE
                     DSGRVTIQGCIFNTEIKTLNNGRTIITFNITDNTNSITAKIFAKEENDFAKLTSNIID
                     GRYIALKGDIQYDKFIKELVVFPISITAAEKSIRTDNAEVKRVELHAHTGMSAMDGIC
                     SASDLIKKAKSWGHKAIAITDHGVVQAYPEAYEASKIHGVKVIYGVEGYFVNDGVPIV
                     YNAVNRSFEEEFTVFDIETTGLYPIKDKITEIGAVKIKQGRITDRFSELINPEIPIPE
                     NITKLTGITDSMVKDKPTIREILPKFLEFAGDSPLVAHNANFDSGFIRAKAAELGLIF
                     NNTIIDTLQMSRVLLGGLKRHKLDIVCEYLGIELENHHRAADDARATAEIMLRFIEMM
                     KSKGINDAADINKLSLSGRSHKAGESYHIILLVKNMTGLRNLYKLISMSHLDFFYKKP
                     RLPKSLLMQYREGLLIGSACESGEVFRNILNNLDENTIEENARFYDYLEIQPNGNNEF
                     LVRSGIVANKGELEAINRRIVELGKKLNKMIVATGDVHFLEPEDEIYRRIIMAGQGYS
                     DADEQAPLHFKTTDEMMEEFEYLGRETAYSVVVRNPNRILEQIEEIIPIPENTYPPKI
                     EGAEEQIREMTMKKAHELYGEILPEVVEKRLDKELNSIINNGYAVMYLIAQKLVAKSM
                     ADGYLVGSRGSVGSSFVATMCDITEVNPLPPHYLCPKCKYSLFFLDGSIESGADLNDD
                     NCPQCGTVLRKEGHDIPFEMFLGFEGDKEPDIDLNFAGEYQPEAHKYTEGLFGKGNVF
                     RAGTIGTIAEKTAYGFIKKYYEEKGIKINNAEANRLIKGCTGIKRTTGQHPGGVMIVP
                     DYTDIYNFTPIQRPADDPSSNIITTHFDYHSISGRLLKLDILGHDVPSIIRMLEDITG
                     FGVNNIPFDDSRTMSLFTSAEALGISLKDIECKVGSLGIPEFGTKFVRQMLMDTQPGT
                     FAELVRISGLSHGTDVWLNNAQELIKSGTATLKEVIATRDDIMLYLIYKGVNPRTSFK
                     IAENVRKGKGLTPEYEEVMREQNIPEWYIESCKKVKYLFPKAHATAYVMMSFRVAYYK
                     VYYPEAFYATYYTVKLEDFDADLFIKGKESIRAKWIEIDKLGNNATAKEKNLMTLIEV
                     VYEMYLRGIKLLPVDIYKSDVKRFMVTKEGILPPLASLQGVGASAAQNIACARQESCF
                     ISMDDLRERARLSKTVIEILKNHGCLEGLPESNQLSLFSAGGNLGV"
     gene            complement(18924..19577)
                     /locus_tag="PKU88_05305"
     CDS             complement(18924..19577)
                     /locus_tag="PKU88_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX68737.1"
                     /translation="MNVTAVIPAYNEEQTIADVVKCVKSIDKIQKVIVVSDGSTDSTA
                     EIARECGADVIELDENVGKGGAIRAGINECGTEIILFLDADLIGLTEKHVLDLIEPVI
                     DNKADMTIGIFKKGRMVTDLAQKVTPYLSGQRAIKKSIIDKIPNIDITRYGVEVALTK
                     YVDKFNVRVQEVDLPDMTHVTKEEKLGIIRGVHARLKMYWDIVKILSSYNIKSGSSK"
     gene            complement(19629..20690)
                     /gene="ispG"
                     /locus_tag="PKU88_05310"
                     /gene_synonym="gcpE"
     CDS             complement(19629..20690)
                     /gene="ispG"
                     /locus_tag="PKU88_05310"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPX68738.1"
                     /translation="MGTYTRKIRIGDRYIGGGEPILIQSMTNTRTQDKAGTIEQIKRL
                     EAAGCDVIRIAVPDMEAAEAVKDIKSRISIPLVADIHFDYRLAIQSIINGADKIRINP
                     GNIGDNERVKKVIYAAKERGIPIRIGINSGSLEKEILEKYGSPTADALVESALKSVRL
                     VESMDFSDIVISIKSSDVLLTIDSYIKASKAANYPLHLGITEAGTLLAGSVKSSVGLG
                     ILLYNGIGDTIRVSLTGDPAEEIYAAKEILKSLKLLKEGIEIISCPTCGRTCVDLIRI
                     TEEVEKRIKGIEAKKPMKVAIMGCAVNGPGEAKEADLGIAGGQGEFLLFLKGKTVRKI
                     PQENVIEELIKEIEALQSV"
     gene            complement(20699..21706)
                     /gene="rseP"
                     /locus_tag="PKU88_05315"
     CDS             complement(20699..21706)
                     /gene="rseP"
                     /locus_tag="PKU88_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPX68739.1"
                     /translation="MTLIAAILVFNLIVFVHEAGHFAAARLGGIKVVEFALGMGPKII
                     GKKFGETVYSLRAFPIGGFCLMLGEDEENNEPGAFNNSPIISKISSVISGPVFNIILT
                     ILIYSLVIAPVAAPIVGQVTKGMPAETSGIKAGEMIVGINDVKINEWKEMKPEIAKHE
                     GEQITVTVEKNGVQREVMLTPVKNPGTDDIVIGITQKVGISGFSIKEGINTTFAVSKM
                     MLSFLGQLVVGKADANEVSGPISILVYINEAAKAGFIKVLYLTALISLNLGIINLLPL
                     PALDGGRLLFLFIELIRRKPLPAEKEGMVHFVGLIALMALSIFLMYRDIIRFNLMDIF
                     R"
     gene            complement(21706..22866)
                     /locus_tag="PKU88_05320"
     CDS             complement(21706..22866)
                     /locus_tag="PKU88_05320"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPX68740.1"
                     /translation="MVKVLSVLGSTGSIGTQTLDIIRENKEEFKIDALAANSNIDLLE
                     AQVREFRPKIAAIGDMSKYKEFKSRVGGMTCVAAGLEGVVEAATLESCDTLVSAIVGI
                     AGLIPTYNAIKTGKNIALANKETLVTAGRIITREVKKNKVNMLPVDSEHSAIFQCLAG
                     SRHNEISKIILTASGGPFRNKTLKELESVTVQDALKHPNWSMGRKITIDSATLMNKGL
                     EVIEARWLFGIMPESIEVCVHPQSIIHSAVEFVDGAVIAQLGLPDMKVPIQYALTYPE
                     RLPMKSAKLSLTSVGELTFYKPDTEKFKCLRLAFRALDMGDSACVVLNGANEEAVKLF
                     LSGKISFLDIGNLIEKTLDKHKIIENMDIEEVSELDRWSRETLAYLYETGCK"
     gene            complement(22863..23669)
                     /locus_tag="PKU88_05325"
     CDS             complement(22863..23669)
                     /locus_tag="PKU88_05325"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPX68741.1"
                     /translation="MLKTRVLSALLGIPLIIFILYSGGLYLYLFTAAVSLTGLFEYYR
                     ALSNINISTNKIFGYTIVILYYIMFLLPIAFNRPGFLIALSVITLLIYEIISQKHSFT
                     EISINIFGIAYIPFLFSHLLFIENLRYGNIILWLPFLTAWFTDTFAYFIGIYMGRRKL
                     CPAISPKKTVEGALGGIAGSVAFSIITGIILNNYGIEIRLIHFIITGFLCGIASETGD
                     LAASYIKRYAGVKDFGNIIPGHGGILDRFDSILFTAPIIYYYFVLIGDLI"
     gene            complement(23681..24403)
                     /locus_tag="PKU88_05330"
     CDS             complement(23681..24403)
                     /locus_tag="PKU88_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPX68742.1"
                     /translation="MLGDIDKGKLPVHIAIIMDGNGRWAQKKGLPRVMGHKAGMEALK
                     KTVKSCSDLGIKILTVYAFSTENWNRPQDEVNYLMDLLVEYMRREVNALHKNKVKIKI
                     LGEVDMLPDQTRTEIEEAIELTKNNEGLQFNIALNYGGRAEILRACRNLIKDLEAGNL
                     DMDSADEKVFSNYLYTSNDPDPDLIIRTSGEQRISNFLLWQGAYSELLFVDQLWPDFD
                     EAVLHSAILEYKNRSRRFGALK"
     gene            complement(24446..24646)
                     /locus_tag="PKU88_05335"
     CDS             complement(24446..24646)
                     /locus_tag="PKU88_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68743.1"
                     /translation="MDNIPLVIGYTLDHALQIIGEKHDIIIDSTSTPFEDKKKERRGN
                     SPIVVRQKAECDKIILTTSCFK"
     gene            complement(24653..25210)
                     /gene="frr"
                     /locus_tag="PKU88_05340"
     CDS             complement(24653..25210)
                     /gene="frr"
                     /locus_tag="PKU88_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPX68744.1"
                     /translation="MIKDIEKNIQEKMEKTLTVFKHELSTLKAGRANPSLLDRITVEY
                     YGAPTPLNQLANIAAPEPRVLTISPWDAKSIPVIEKAILKSDLGINPSNDGKMIRLVI
                     PQLTEERRKELVKVVKKLGEESKVAIRNIRRDANEHLKKLKKDGDITEDDLKKSEGSI
                     QKITDKFIKEADKAIEAKEKEILEV"
     gene            complement(25215..25928)
                     /gene="pyrH"
                     /locus_tag="PKU88_05345"
     CDS             complement(25215..25928)
                     /gene="pyrH"
                     /locus_tag="PKU88_05345"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPX68745.1"
                     /translation="MSDVKYKRVILKISGEALAGEKSFGLDHDMLLDVAERVKLLREL
                     NVEVAIVVGGGNFWRGRTSEGMDRTTADHMGMLATVINALALQDALEGINVPTRVQTA
                     IEMRQIAEPYIRRRAVRHLEKGRVVIFASGIGNAYFSTDTTAALRAAEIEADIILMAK
                     KVDGVYDSDPVINKEARKYDKLSYMEVINKGLGVMDSTAASLCRDNKIPILVFDLKNP
                     DNIIKAIMGEKIGTVVKED"
     gene            complement(26040..26687)
                     /gene="tsf"
                     /locus_tag="PKU88_05350"
     CDS             complement(26040..26687)
                     /gene="tsf"
                     /locus_tag="PKU88_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPX68746.1"
                     /translation="MISASAVKELRERTGSGMMDCKKALTESNGDMEKAIEILREKGL
                     AAAAKKAGRIASEGIVDSYIHGGGRIGVLIEVNSETDFVAANEEFRQFVKDMAMQVAA
                     SNPLYVKREDVDPKVIEKEREIYKAQALNEGKPEKIIERMVEGRIEKFYKEICLLEQP
                     FIKDTDKTVQEILTSLIAKIGENITIRRFVRFERGEGLEKKEDNFVDEVMSQVNK"
     gene            complement(26754..27452)
                     /gene="rpsB"
                     /locus_tag="PKU88_05355"
     CDS             complement(26754..27452)
                     /gene="rpsB"
                     /locus_tag="PKU88_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPX68747.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTVKKVEEAYNFIKEVAASGKDILFIGTKKQAQESIESEAIRANMFYVNQRWLGGMLT
                     NFKTIRKRIERLNELEKMEEDGTFEVLPKKEVINLRAEKEKLQKNLGGIKNMGENPGA
                     IFVVDPRKEKIAIQEAHILGIPIVAIVDTNCDPEEVDYPIPGNDDAIRAVKLLTSKIA
                     DAVIEARQGEQLAE"
     gene            complement(27657..28058)
                     /locus_tag="PKU88_05360"
     CDS             complement(27657..28058)
                     /locus_tag="PKU88_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68748.1"
                     /translation="MGNYNLKNIILGLGIGLVLASMMNISMGNKELTLEEIKKEAQKH
                     GLVVFSKEEIIDSRNQENTAPTEKVTVEIKSGMSSESIAALLRQKGIINDSGAFIDKL
                     KETNNETKLKEGTFEIPKGSGYDEIISILTK"
     gene            complement(28058..28567)
                     /locus_tag="PKU88_05365"
     CDS             complement(28058..28567)
                     /locus_tag="PKU88_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX68749.1"
                     /translation="MINYVLMAIGVFVILAASIMIFLDKVKGEDIYFSMDVKEQEIKK
                     VIEDADEIISELNYTSEMVVQEIEEKLSSFREELKSLNNQVPGTSSAENKPAAPVVPN
                     VSAANKKKKIINDNSDLDKPVDEKLSQKQLKVFEYAAQGMSVTDIAKQMNIGQGEVML
                     ILSLKNEVE"
     gene            complement(28581..28901)
                     /locus_tag="PKU88_05370"
     CDS             complement(28581..28901)
                     /locus_tag="PKU88_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68750.1"
                     /translation="MSLRPVDLQTIIPKLPEVQKAKNAENELEKNNLSINMHKEQQQQ
                     DKNTQQIIETKKAQEARINRDGKQNRGQGDQKGEHKDSRPEDNEGQENKKNKDPMSTI
                     DIRI"
     gene            complement(28924..30732)
                     /locus_tag="PKU88_05375"
     CDS             complement(28924..30732)
                     /locus_tag="PKU88_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPX68751.1"
                     /translation="MSNEYIILEGKDYEKLIAKGMEHFNARKEELNIEILESKKNLFS
                     SYYKVKIAKGENKTLEIIESNIDSILNENLKANTKSIDFDFREDGVYIKADKDVTIVD
                     IVTKIDLRRIQSVDLTKIKQMLEIGSLDNWIKIAEPQMEKKIDSECLVKTAKDKMKAF
                     IVISKPIGGKDITLEVINEALKQEGVKFNIKEDVIEKAVSQKVYDKEILVAEGIEPQP
                     GKDAELVYHFDTSAEKSIGIDKDGKIDYHELSLIKNVKAGEKLVTLIPHTEGIPGKNV
                     SDEDVPGKDGKKLALPRGKNVVVSEDELELVAAIDGEVKIIDGKVNVFSVYEVKNNVD
                     NSTGNIRFNGKVIVMGNVLTGFLIEAEGDVEVYGVVEGAQIKSKGNIILHRGIQGMNR
                     GELYCEGDLVAKFIENSKIDVKGNIQSDAIMHSQVTCGKKLEATGRKGLLVGGTFKVG
                     EEIRAKVVGSPMATITELEVGVNPEMRNKFEELKNELKQVTNNLDKTSKAVDLLTKMS
                     KSMELPEDKKALQAKSLQLKLQLKQKQDQLLNEQNELEVYFEELSKGKVKVYDIIYPG
                     SRIIIGSSMMYIKDSIKFVSFCRMHAEIKMLSYNDI"
     gene            complement(30749..31483)
                     /locus_tag="PKU88_05380"
     CDS             complement(30749..31483)
                     /locus_tag="PKU88_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPX68752.1"
                     /translation="MANLWEVYSKTKAAEIREELILKYTYIVKYVAGRLYSSYGNNVE
                     FDDLVSYGIFGLIDAIDKYDIARGVKFETYAQLRIRGSIIDHLREIDWLPRSIRQKSK
                     ELEKSYNDLESRLGRPATDEEMAESFGLSVEEFQKKIQNITTYSVVSLDDLLEQKREI
                     ISNEADKHSETPENVIEDKEMKEILADAISALPEKEKKVVSLYYYDELTYKEIGKLLY
                     ISESRVSQLHTKAIIRLKNKLKVAFE"
     gene            complement(31515..31883)
                     /locus_tag="PKU88_05385"
     CDS             complement(31515..31883)
                     /locus_tag="PKU88_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68753.1"
                     /translation="MRISDFIPILIVITALIVTVTLGICNNIDFSELMIRCMAVTIVF
                     GIFGSLITKTVLNLIEYYRVSIHIQDKGGTVSATGSKLDIKVPPLDDEALEAIDYDSD
                     NEFIEVNPADLSKYINSNTD"
     gene            complement(31917..32402)
                     /locus_tag="PKU88_05390"
     CDS             complement(31917..32402)
                     /locus_tag="PKU88_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPX68754.1"
                     /translation="MIDIVKVGMADLNTTFCPGVLTTLGLGSCVGICLYDPSTKIAGM
                     VHIMLPSSAQIKNNSNEAKFADTGIVKLVTEMIKIGAKRNKLVSKIAGGAQMFNFNDA
                     SEVMRIGLRNVTATKDTLKMLNIPLISEDTGGNYGRTIELYSENGILLIKTIGYGTKQ
                     I"
     gene            complement(32404..33018)
                     /locus_tag="PKU88_05395"
     CDS             complement(32404..33018)
                     /locus_tag="PKU88_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HPX68755.1"
                     /translation="MGISIEELNSLQIDVLKEIGNIGAGNAATALSKMISKRIDMDVP
                     KVNILEFKNVAELVGGPETEVVGIYFKVNGDINGSIMVLVDKKSAKMLANLLMGRDSN
                     GDDLDEMDLSALQEVGNILSGSYLSSLSTLTNLNLTMSVPSLAIDMAGAIISVPVILF
                     GQVGDRVMLIETDLFEGTYHVKSNFFLIPDEDSFEILLKSLGVF"
     gene            complement(33033..33476)
                     /locus_tag="PKU88_05400"
     CDS             complement(33033..33476)
                     /locus_tag="PKU88_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPX68756.1"
                     /translation="METATFARQFVIFKLENEEYGIDILRVKEIKEMMSITRVPNTAH
                     YVRGVINLRGEVIPVIDLRKRFNLPEGKDSNNTRIIIVSVDDIIVGFTADYSSEVVEI
                     SSDAIEEAPDGVGSVDQGYISGIGKVGERLIILLDIVKIITNPAV"
     gene            complement(33490..35505)
                     /locus_tag="PKU88_05405"
     CDS             complement(33490..35505)
                     /locus_tag="PKU88_05405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPX68757.1"
                     /translation="MDVNQYMEIFIEESLEHLQELNQNLLSLETNPGDMKTLNEIFRV
                     AHTLKGMAGTMGFTGIANLTHQMENVLDAIRTGNIEVNSSMIDILFECLDYLSNSINT
                     ISATGKESSSNVNSIIAGLNGMLAKKPEAEASDTADKKEPQESKMKFNQYDDNIIRKA
                     VQQNHGVYKITVVLAKDCMLKSARAFLVFQTVERYAEIFKSEPKVEDIEDEKFDNEFT
                     IAIITKETEALIRKELESISEVTEVIFERIEVPEELDVKDIDEITNEEKETLKYSIED
                     KENGDYIQGKKARMGKTVRVDIDRLDKLMNLVSELIIIKTRLEGVGTDSKNQDMIEAV
                     EYLERITTNLHDAVMKVRMVPIERVFNRFPRMVRDLAKSLSKDIILNMSGEDTELDRT
                     VIDEIGDPLIHLIRNSIDHGIEEKEERIKNNKPANGTVNLRAYQDGNSVVIEVEDDGA
                     GIDIEKIKRKAVERGILTSSEADDLDEKSAIELLFNPGFSTADKVSGLSGRGVGLDVV
                     KTKIESLNGLVEVENYRGKGSKFIIRLPLTLAIIQALLVNIGEEKFAIPLNVIRDIID
                     IEASTIRNVHGQDVVLNRGNVLPIVRIAKMLGVINASSDDSDQLTAVVVKKGEKNAAF
                     IVDSLMGQQEIVIKTLGKFLSGIRNIAGATILGDGQVALIIDSNSLI"
     gene            complement(35510..36592)
                     /locus_tag="PKU88_05410"
     CDS             complement(35510..36592)
                     /locus_tag="PKU88_05410"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPX68758.1"
                     /translation="MLSGKEKIRVLIVDDSAFMRVLISDMLKNDADIEVAGTARNGKE
                     GLEKAILLKPDIITLDIEMPVMNGLEALVELMKLKPVPKVIILSSLSYEGGEAAIKAF
                     ELGALDYVVKPVRSLITIDAEQMREELINKIKSICWSKTGYFPQIKPEYKPPSVYKKV
                     KHKSRSDEYLKYIVAIGTSTGGPKALQEVVTKLPEDIPAAVLIVQHMPPGFTKSLANR
                     LDSLSEINVKEAEDGDVLTSGWAYVAPGNYHMLVKSHEDNKYKILLNSEAPENGLRPS
                     VNVMMKSVAETGHDKIIAVIMTGMGSDGSEGILEIKNAGGKTIAQDERTCVVYGMPKS
                     AVNTGAIDTIAPLHGITGEILKCMGV"
     gene            complement(36594..37268)
                     /locus_tag="PKU88_05415"
     CDS             complement(36594..37268)
                     /locus_tag="PKU88_05415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HPX68759.1"
                     /translation="MKGLTKYFNIGDKILIEYFDAVGELRQYVSQIVDIHDGQYEEFF
                     DVLIPIYKNMDVNIRQGTVLKLIVFKGEAIYEIRTILFKKLFGSIPLLRLKAASEVIK
                     KQRRDYYRLKIFREIEARLVINPEEKQYGEKFKCTIQDISAGGLMFSSREYFNENDTL
                     EFALDLNGPIMVVQGVIVRRILNESYRLLYTYGVQYTNLNMADRNAITKFIFEEQRRL
                     IKKGLI"
     gene            complement(37345..38247)
                     /locus_tag="PKU88_05420"
     CDS             complement(37345..38247)
                     /locus_tag="PKU88_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPX68760.1"
                     /translation="MKDQAERLREIISSIKNYKDEEKRTGILTDKPGSRVIAVTSGKG
                     GVGKTNFTVNLGIKFAQMGLRVVIIDADLGLANVDVVMGKMSKYNLSDVINSNKNILE
                     ILEEGYEGVKFVSGGSGVQELVKLNRTQLVDLLMKLGRLDEEADIILIDTGAGLSENV
                     ISFVHAAREVILVTTPEPTSITDAYALIKTITHKDKTKNIKVVVNRADNAAEAFNILD
                     KLNIVTQKFLGVKLQKLGYILNDACVTKAVKIQQPFVISFARSEASKNMNDIALTLMD
                     NKELSPSSVSGIKMFINKLTSLFN"
     gene            complement(38231..39439)
                     /gene="flhF"
                     /locus_tag="PKU88_05425"
     CDS             complement(38231..39439)
                     /gene="flhF"
                     /locus_tag="PKU88_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPX68761.1"
                     /translation="MKVKRYVGETAQEAMQKVKSDLGRDAIILNTRKIRKKGLAGLFS
                     KPLIEVVATIDNDISSGARTNNARNDHTIETNEFIKELNENLQVQKNSLKDNTKKHTH
                     LDDKELIQQDFMELKNMLNKVYDAVKVDYENTKLSDVVKKYLKTLEDNEVDKVIINEI
                     KEKLMETLDSEKQQSDAIVRNTVYNILMKYVKEPEPFSFAGGKKVIIVIGPTGVGKTT
                     TLAKLAANMVLTEKKRVGLITSDTYRIAAVEQLKTYSEIIGVPLSIIYTPGEILNAIE
                     SYAEKDLILVDTAGRSHKDKYQLMELKSLIKSSINYEVYLVISATTKFSDCIEIIKNY
                     SFLDDYKLLFTKLDETSAFGVILNVAYITKKPISYITTGQSVPDDIEIADNGKIINCL
                     LGDKLYERSS"
     gene            complement(39439..41466)
                     /gene="flhA"
                     /locus_tag="PKU88_05430"
     CDS             complement(39439..41466)
                     /gene="flhA"
                     /locus_tag="PKU88_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPX68762.1"
                     /translation="MGRYSDVIAVLIVALIILIVIPVPTILIDILLTLNISLSLVIFL
                     LSMYIKEPLQFSIFPTILLITTLFRLCLNFSTTRLILGKGYAGEVINAFGSFVTGSNP
                     IVGFIMFLILVIVQYIVITKGAERVSEVSARFTLDAMPGKQMAIDADLNSGLINEMEA
                     KERRKAIQQEADFYGAMDGASKFVKGDAIAGIIITVINIVGGFAIGMLQMDLDFIESL
                     STFTNLTIGDGLVTQIPALLVSIAAGMVVTRAASNSDLGTDIIAQLLSEPKVLYIASG
                     ALLILAFTGLPPVPNILLASILAYMGYTLRKTIKAAQKEEEIKAQDVEVEEIRKPENV
                     MSLLQVDPIELEFGYGIIPLADVNQGGDLLDRVVMIRRQCALDLGLIVPVIRLRDNIQ
                     LKPNDYVIKIKGNEVASGELMFDNYLAMNPGTADGELDGVKTTEPAFGLPAVWISENQ
                     KERAEMMGYTVVDPPSVIATHLTEIIRNHAHELLGRQDVQKIIDNVRENYPALVEDVI
                     PKQLSIGDIQKVLANLLKEGVSIRDMVTIMETLADYSPMTKDTDMLTEYVRQSMRRNI
                     TKRFIVEMQAKVITLDAVLEQTIMDSVQQTEYGSYLNLEPNTAQRIINNLLKEMQKLT
                     SLGEQPIILASPVVRLYFKRLTEQVAPGLIVLSYNELDPLVEIQSVGVVSI"
     gene            complement(41481..42572)
                     /gene="flhB"
                     /locus_tag="PKU88_05435"
     CDS             complement(41481..42572)
                     /gene="flhB"
                     /locus_tag="PKU88_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPX68763.1"
                     /translation="MKDYAYLKINLQLFGEKTEPATPKRRQELRQKGQIPRSRELVTA
                     VVLLISFFSMRFLSKFIFGDLIFAVRNSLSFPKDIDSRFTTNEIMLIFLQNLLIFAKI
                     LAPILLIVALCVIIMNYVQIGSVLTAKPLMPNLNKINPVEGFKRLFSKNAYVELLKSI
                     FKIGIIGFIIYDYLVDNYRVIPELLNMNVESTMVFIGNTIISVGIRAAAVLLILSVFD
                     YGFQKWNFEKEIRMSKQEIKDEYKLIEGNPQIKSKIKEKQRQLSLRRMMAEVPSADVI
                     ITNPTHFAIAVKYEEAVSEAPVVIAKGKDLIAEKIKDTAKKNNVPIVENKPLAQALYK
                     SVEIGQQIPSELYKAVAEVLAYVYSLKDK"
     gene            complement(42569..43360)
                     /gene="fliR"
                     /locus_tag="PKU88_05440"
     CDS             complement(42569..43360)
                     /gene="fliR"
                     /locus_tag="PKU88_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPX68764.1"
                     /translation="MIMDSISVVLVDRFLVLLLVFIRMTSLFVITPVFGRREMPAYLK
                     IGLAFFCSYIMVPLLGDVRIDYSNIFSFSAIIAKEFLVGIIIGFVSFMVFSALYLAGQ
                     IIDMQVGFGMVNVLDPTTNTQIPMIGNFIYIMTTILFLAIDGHHVLLAALYKSYSVLP
                     IDGFAFTEAMTNNIAAIFTEVFLIGFKISIPVMAATLLAEVALGILSRTVPQMNVFIV
                     GLPLRIGIGLITLYIMIPVFIQIITVTFDRMYGYVYLIIRSMAKG"
     gene            complement(43357..43626)
                     /gene="fliQ"
                     /locus_tag="PKU88_05445"
     CDS             complement(43357..43626)
                     /gene="fliQ"
                     /locus_tag="PKU88_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPX68765.1"
                     /translation="MDQGIVIDVAQEAIKSVLLVSAPILGLGLLVGLIVSIVQATTQI
                     QEPTLSFIPKIIAVSLTMLVFGPWMMNIMYEFTVRLFENIPVYIK"
     gene            complement(43641..44405)
                     /gene="fliP"
                     /locus_tag="PKU88_05450"
     CDS             complement(43641..44405)
                     /gene="fliP"
                     /locus_tag="PKU88_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934844.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPX68766.1"
                     /translation="MYKKNGIIKIILILVLMILMGIIDVWCEPLPIPKIGLDIETAEN
                     PQDVALSMQILFILTIISLAPSILIMLTSFTRIIIVLSFTRNALGTQQMPPNQVLIGL
                     ALFLTFFIMSPVITEINENAFTPFTNGEITQEAAIEKAAEPIRNFMFKYTREKDLALF
                     LNLSQVKQPVAKIDIPTSALIPAFIISELKTAFEIGFFLYIPFLMIDMIVSSTLMSMG
                     MMMLPPIMISMPFKILLFVMVDGWNLIVRSLILGFN"
     gene            complement(44398..44799)
                     /gene="fliO"
                     /locus_tag="PKU88_05455"
     CDS             complement(44398..44799)
                     /gene="fliO"
                     /locus_tag="PKU88_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPX68767.1"
                     /translation="MNTGSTIYGILYFLFMSAVILAAAYYATRFLSKRSLKNAGNKNL
                     KIVEAAPLGIDKSILLVKVGEQYLLLGSTQKSITLLTEVDPEKLAVGDDESIEVYMSS
                     FQDENKKNGMNSIKNNLNKLKSIVRGNKTDV"
     gene            complement(44815..45177)
                     /locus_tag="PKU88_05460"
     CDS             complement(44815..45177)
                     /locus_tag="PKU88_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX68768.1"
                     /translation="MANGILIVDDAAFMRMMIKDVLSKNGFEISGEAENGAKAIEKYK
                     EINPDLVIMDITMPEVDGIQAVKEIKKINSAAKVIMCSAMGQQAMVIEAIQAGAKDFI
                     VKPFQAERIIEAVKKVLG"
     gene            complement(45213..>46286)
                     /gene="fliY"
                     /locus_tag="PKU88_05465"
     CDS             complement(45213..>46286)
                     /gene="fliY"
                     /locus_tag="PKU88_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HPX68769.1"
                     /translation="LTTEEIDALGEIGNISMGTSATTLFTLLNNKVSITTPKVSVVTW
                     EELSIQYPIPYVAVQVQYKEGLEGSNLLILKEDDVKIITDLMMGGSGEISPDSVGDLQ
                     LSAIGEAMNQMIGSSSTSLSSMFNKRIDIYPPRAFILYFQNLSELEQFNSADKVVKIA
                     FKMEVGDIIDSEIMQLIPIDFAKELVRSLLDNISTESEPIFVGKAEEPQVKQPAVSDP
                     GTQPQKPESNTILSKGYEEREEYEPAARREERKVVNVQPVQYQTFDVEKLNAEKESIE
                     LIMDVPLEITVELGRTKKLIRDILEFSPGTILELDKLAGEPVDIMVNGKYIAKGEVVV
                     IDESFGVRITDIVSASKRLSRIQ"
BASE COUNT        11491 a        10892 c         7915 g        15888 t
ORIGIN      
        1 acatgtggga tcttcgactg ttttatcttt tttaagtaat tctcgcaata aatgtgcatt
       61 tcggggaaaa gaagcctgat aatgataaca cgctccacaa tataaaccac atactcctaa
      121 caatttataa ctcaacaaaa agacctccta acatttttaa taataattca aaaactatca
      181 atagccttcc ggatacaggc acatattgct tttcgaacat gtcgttctaa cgtggcgcgg
      241 gctttgcgcc cgctgcgtga agccattgtt aggcgatggg cagagtatgt tcttagcttc
      301 tttaattatt tttaaccccg cccattctgc tgaagcgcag gacgcgcgta agcggtttct
      361 aaaaaccata cttatctttt ctaagtttaa atgctttttc tccaccctcc ataatctttg
      421 taataacgtc cctaataatt gatatatcta ttgcaccaag atgctttatt tcaccattag
      481 actgatctcc tgaaactcta taatcacaac aagctactag ttcagcgtca ccattgacaa
      541 caatattcgc attatggctt gtaaaaatca gttgacggtt tttctttgat ttgcatatta
      601 gttcagctat ttcggaaacc atctgattat ctaaatcatc ttctggttgg tcaattatta
      661 gtgttgggcc ttcttggttg agtaaaacat gaataagtga tgttgcttgc tgacctgcag
      721 aggcatcagt aaattcaata tattcgcctt cctttgtttt gtattcaaaa actggaatat
      781 cttctagttg ggtaagatac atatttatga aattgttgat attcattttt tctgcaattt
      841 ttctcaaatc attttgtgat aagccaatag aaactaattt cggagtcgaa ggaaaggtct
      901 caggggaatc attttcaaaa tcgaaatttg caaggctctc aaactcattt aaaagttctt
      961 gccattcatt aatgggattg gttgcgtttg aaacaaatga acatattgaa tcagtctttt
     1021 ctttcctgat atttgaccct gccaaaatac tccttaatgt cgaaccaagt gagtctatac
     1081 ccttgcctct cccaagggtt gcgcgtaaat tattatctga taagatggat aattgctcac
     1141 attgagcctg gatagtatca gctctaccat tatgcgaaga aacccattgt tcttttagtc
     1201 ttgaaaattt ttgatcttgg tcgcccaatt tgtttaataa ttgctttttt tccccaaggg
     1261 atttgcgaat ttcattagat cttttttcga gttgggatat ttgtgttaaa gttgcttgct
     1321 gagaagaaga tcgctctttt gctgattcat aatgctgttt atgtattaag agaagttgca
     1381 tccaatcagt tcttccatgc gagaattcat tttctgcggc actatattcg gctatattct
     1441 tcttgatatc gtttaattgt tttttaacat ctttaaatag ttgagaaata ctatcattga
     1501 tggtcttgat ggtatccttt gaagcatccg gaattataag tctcggtgga atgggtgtag
     1561 gaaaagcttc aatttcagtc aaaagtacat ccaaagaatt ggatgcagct gttagttccg
     1621 acacccattc ctcaactaat tgttctgttt cagaataggc ttcgtgagta tcaattgtag
     1681 ctctatcttc atctgaaata cctttaagat tttttctaag attttctacc tgctcagtta
     1741 cagatttgag ttcaagttct ctgcggtgta tctctttttg taatagattt atttgatttc
     1801 ttttctcaaa acaagcccta atatcggtat taaggttctt aaaatgcaag tcatactcag
     1861 aaaggttctg cctaatgggg ctatacacga gtcgcttcaa ttctgttcca ctaacaccaa
     1921 cactactaag ttgtttttgg ctatacgctt gaatcggtaa tagatttctt atattttctt
     1981 cagtacatac ctcaaaatct tcatttccta tttttagcaa aatttcccca gattttgctt
     2041 ttctgcgaac aacatgtaca ataccgtttt taataaagga aacctgaact gttccgtcat
     2101 aaggtaaaag cgttctttcg attaactttt ttcgtctttc ctgataattc ggaagttctt
     2161 cgtcctcctg tgataaatca ggatattggt cacataaacc ccacctgaga tattccaaaa
     2221 cagtagattt acccgttcct cttcctccaa taatggcatt gtattggcga ttgaattcaa
     2281 ggtaaattcg acctagaaac ttactgtttg atacatccag tgaagtaatg aatacaggcg
     2341 gtagaacagg tatctcttga gaaatacggg agttttttgc aagacaggct tgtcgtagcg
     2401 cttccgcagt tggctcagcc cactttatcc aagtactatg tctaccaagg tcttcatgtg
     2461 tcgccttgcg attatcagac gtttgaatta gtgcaatttc tttgaaacca taatgcctgt
     2521 tttttccatt aactatgtcc gtattccctc gtccaagagt agaaatggaa ctctctaaat
     2581 aaccgccaac gcaaggcatt gttttataaa aatcagcaaa accgcttcgt aataatgttt
     2641 tgtggccatt atcactgaca tgagggataa tagtgaatct gcccttaata taatcaagat
     2701 tacttagttt ttcgtataat tcacagaaat tttttgtgac agcttgcggt atcggaacga
     2761 ttgttgcagt tcttgattca gtgtcaggat ttggtgtaat tcctagtgtg gttagtactc
     2821 cctgtaataa gttttctgga aaagcactgt caagaattaa taacgcttgg caaggtgggg
     2881 atgcaagagt taattctaat ccaggaaaaa ctattagttt ttcctcatcg ggataacaca
     2941 ccccgttatc atttaattca gctaaagcgg cttgtttaat ataagggaag aaaacaaaat
     3001 catgatgatc agttatggca attgcatgta atccttttgt gcgacaagct tgtactaaag
     3061 tttcagcata tgcctttctt tcctcatttg aaatcgcttc gggtccatgc caattaatat
     3121 cacgcggtga atgtacttga aagtcacatc tataaaagtg agccccctta tccattctat
     3181 cctctcctcc tttttgcccg ccatggacgg cgggcgtctt aggcggggtt aaaaaataca
     3241 cccttaatgt ttataaatct attatcctaa actattttat cgctctgcct ttcgcctaac
     3301 gtggcgcggg atttacgacg ttcacgagcc ctagtgcgac cgcacttggc gagtgaatgt
     3361 gcgtaaaggc tgctgttagg cgatgtggcg cttcgccact gcctaacagc gggcgcaaag
     3421 cctgcgccac gttagagcga atggagcgaa gcgacatgtc gctctaactc ctgcaactac
     3481 gtatgtcgtc aatacatcca aaatcgtgtg tgttcacgaa catatttcgt caatattttc
     3541 atttatctgc gaatccactc tatataaatt ctccatattt cgagataaat ccttccaaat
     3601 aaatacaaaa taagtatgag tccatcatac ttatctcaga tatgttcttc agttgtgctg
     3661 caaaagaaaa aacataaacc ccttggttta tgctttttct ttttcttctt ctgccttctc
     3721 ttcttctttt gccacatcgg ccattgcgtg ccgtcttgaa aggttaattc ttccctgctt
     3781 gtcgatttcg gttactttta caagtatctc gtctccgact gctacaacat cctcaacctt
     3841 ttcaactcgt tttgtatcaa gcttggagat gtgtacgagg ccttccttgc cgggaagtat
     3901 ttcaacaaat gctccgaagg tcatgagcct ggtaactctg ccgagataga tttctccttc
     3961 ttttacttcc tttacaaggc tttcgattat tttcagcgcc ttgtcaccgg ctgcctgatt
     4021 tacagcagcg atgaagactc ttccgtcatc ctcaatgtct atctttacgc ccgtctctgc
     4081 gataatcttg tttattgtct ttccgccggg gcctattatg tctcttatct tgtcaggatc
     4141 aacccttgtt gttattattt taggagcata aggtgacagt tcttttctgg gttcgcttat
     4201 tacgctgagc attttgtcaa gtataaagag ccttcccact cttgcctgct ccagtgccct
     4261 ttccagtata ggcctgtcaa taccggctat ttttatgtcc atctgaatgg cagtgattcc
     4321 cttgcttgtt cctgcgacct tgaaatccat gtctcccagg aaatcctcca tgccctgtat
     4381 atcggtaaga atggctacat tgcccttctc cttcataagc cccattgcaa ctccggctac
     4441 aggtgccttt atcggtaccc ctgcatccat caatgccaat gtgctgccgc agactgagcc
     4501 ttgtgatgtg gaaccgttgg agcttactac ttcagataca agcctgattg tataagggaa
     4561 ttcttcttct gatggtatca ttggctccaa tgctctttca gcaagggctc cgtgtcctat
     4621 ttcccttctt cccgggcctc tcatgaatct tgtctcgcct acgctgtatg gcgggaaatt
     4681 gtagtgatgc atgtatcttt ttgattcttc ttcggtaagg ccgtcaagta tctgtacatc
     4741 acccaaagcg ccaagagtcg caattgtaag aacctgtgtc tggcctcttg taaaaagccc
     4801 tgaaccgtgt actctgggaa gaactccaac ctcgctgctt atgggcctta tttcgtcaag
     4861 ctttctatcg tcgggtctta tgccctcctc tgttatcagc tttcttacct gctccttgag
     4921 tatgtcatat aacacctcgt caatgtcctt ttctttctca gggaagattt ctgcaaaatg
     4981 ttcaaaggtc tcttctttaa ctgcgtcaat tttctcctgt ctttcaagct tatcgggagt
     5041 ccttatcgca tcaagggtct tctgggttgc atattccctt acggcagttt caatttcttc
     5101 ttccggcttg aaaacagtgt attctttttt ctccttgcct actttagcaa caatttcttc
     5161 aataaatgca acaatctttt tgatattgtc atgagcaaac attattgctt caagcatgac
     5221 atcctcaggt acttcctttg cacctgcctc aaccatcatg acagcatcct tggtgcctga
     5281 tactaccaag tgaaggtcgc ttttttctct ttcttcgtta tttggattga gtactaactc
     5341 tccgtcaact cttccaacca ataccgagcc tgtaggcccc tggaagggta ttgttgaaat
     5401 ggaaagcgca gctgatgaac ctatcattgc tgctatttca ggcaggttat cctgatctac
     5461 ggataaaacg gtagccacta cctgcacgtc gttcctgtat ccgtgcggaa acaaaggcct
     5521 gatcggtctg tcaatcaatc ttgctgacag tattgctttt tcggtaggcc ttccttccct
     5581 ttttatgaag ccgcccggaa tcttaccgac tgcataaagt ctttcttcat agtcaacgct
     5641 gagagggaaa aaatctattc cttcctttgg ctctgccgaa gcgcatgctg ttacaagtac
     5701 gacagtatcg ccgtatctta ccaggaccgc tgcatcggct aactgggcta ttcttcctgt
     5761 ttcaatactt agctttctcc cagccagctc aagttcaaat acatccatgt attttcctcc
     5821 tttcattttg tatattaagt ataatctgaa accgggcaaa aacttttcct gttccagaaa
     5881 acttactcca tatgtattta gtgtatccat gtcaaatcaa aataattgca aaaaggacgg
     5941 gaatgatccc cgccctcaaa ctactatttt cttagaccta gtttttcaat aatagcacgg
     6001 tatctctcaa tgtctttagc cttgagataa ttcagaagtc ctcttctctg tccgaccatc
     6061 ttaagaagcc ctcttcttga gtgatgatcc ttcttgttga tctgaagatg ttcattcagg
     6121 ttgtttattc tctctgtaag tattgcaatc tgtacttcag gagatcctgt atcaccttct
     6181 ttgatctgat acttctgaat cagttcgcct tttgattcct ttgtaagtgc cattaattcc
     6241 acctccttat aaatcccgaa tatatctcca aaagccaagc caaccgttgg tgaatcggta
     6301 cacctagcta ttggcatcat tttgacaagt atgattttaa catatatagt tctccgtgta
     6361 aaggatattt attctagcat catctatgat tcaaatgtgt atctgtcctg aatgtcgcgg
     6421 tttatctgct cgacaagtcc ttctatactg tcaaacttca tttcatccct cttatgcttg
     6481 tggaagaaaa tctcaatttc ctccccgtaa atatcgtcgc aaaagtcgag aagatgcgtc
     6541 tccacactca ggctttcacc gttaaaggtc gggttaaagc ctatatttgt tatgctttta
     6601 aaaagccttc cgcatacttt tgtactggta taataaactc cctttttagg aagtgttata
     6661 tccttcttaa cgtctatatt ggcagttttg aaacccatta actcaccatt ttgtttaccg
     6721 taaacaactt caccgtgtac tgaaaaatcc ctgccaagat attgtgacac tttgtcaaca
     6781 tttccgcttc ttattacgtg cctgatgagg ctgcttgaaa ctatatggcc gtttacctca
     6841 aaggcatcaa ctatgattac cctgaagccg tatttttcgc cgtattgctt aagatcatcg
     6901 acattgccct ctcctttgat accaaatttg tagttgtatc ctattacaac ggattttaca
     6961 ctaaaacggc ttacaagtat gtctttgaca aaatcctcgg ctctcatttc cctgattttg
     7021 ttgaaatcct caaggtacag catgtcaacg cccatctgct caattacctc agaccgcttt
     7081 ttattgtcgg taatcagata aatgtgctta ttgggtatga gaattttcct tggatgtgta
     7141 tgaaaggtat ataccatgct tcctgtaccg tgaagcctcg attcttcttt taatatctca
     7201 atcagctttc tatgccctat atgaacaccg tcgaagcttc ccaatgcaat gcaccacggc
     7261 gctgacttca tagcttcatc atactgttct gctattttca agataaccac ccttataaca
     7321 agatttcaat aagggttttt gcacgtttgc aaaaacctgc ccgagcgttt caacgaggcg
     7381 ggaaatatac tcgccgcctt ttggaggaac attttattcg cctcgttatt tacaagaatt
     7441 aaatgaatat attttttatt tttataaata gcctctgtcc gctttcatca ttctgcacag
     7501 tgcctgttcc tataaaaccg gcttctccgt atatgcggat gtcctcgttt tcattaagcc
     7561 cttcaaaacc gttcagcact ccttgttcat acaggggatt gccgtttgtt atggatttaa
     7621 gggcggaagc ttttacagta attgatttat aatgcttgaa caggatttcc acattaataa
     7681 gaatgtcatt tatccggttt tcttcagccg ctgctttaat ctcctccagg gtataggaat
     7741 cctcaatcga aaagggagag gagccaagcc tgataagctg tgacatgtgt gcgccgcagc
     7801 cgagggcttc gcctatatca tggcaaaggc ttctgatata ggtgcctttt gagcataata
     7861 tatcaataat agcttcatct tccgatatca tttccaccag cttaagctca tgtatttcca
     7921 caggcctttc ggctatccgg atttctttgc cttcaagggc atattggtaa agcttcttcc
     7981 cttttatttt tattgcggaa tatacgggag gcttctggct gattgctcct gtaaagcttt
     8041 tcaatgtgtc ttccaaagct tttttgtcga ttaccaccga ttcatattct ttgatgacct
     8101 gtccgtagct gtcaaaggta tcggtgacag tgccgaattt gatattggcc ctgtagcttt
     8161 tttgcttgtc agtcaggtat tggactgcct tcgtggcctt tcccacgcag acaggaagca
     8221 ctccggcggc ttcagggtcc aaagttccgg tatggccaac cttctttata ttcagtactt
     8281 tccttaaata ggacaccaca tcatgagagg tcattcccgg cggcttcaaa acatttatga
     8341 ttccgttcat aatggctccc taccttaatt cctctgcgac tgcttccagt atcttttctt
     8401 tcacttcggc aattgtgccg tgaactgtgc atcctgccgc atgcacatgg ccgccgcccc
     8461 caaaacggag agccacggct gctacgtcgg ctttcttttt tgacctcagt cctactttta
     8521 ttattccggg ctcgatttct ttgagaaaca tggctacctc tacacagcca atatctcttg
     8581 caaggtttat tatgccgtct gtatcctctt ccaatgcatt tgcctctttc atctgctcca
     8641 atgaaaggct tatgcaggat gcggcatcat tgtgaaagaa ctccagagtg ctgagggctg
     8701 tcttcataag cattaccttt tcctttgagt tgttctggta tattctttca aacataaggg
     8761 gcacactgac cccgttgtct attaggtctc ctgcaatttg gtgcgttacg gaggttgtat
     8821 tgctgtattg gaattgaccc gtatctgtta caattgcagt gtacaggcat tcgcatatct
     8881 ccctgtccat gccgatatcc agagacttta tcagttcata tattatctcc cctgaagcgg
     8941 ctgaaccggt atctacaagg tttaaactgc cgaactcctt gttgcttata tgatggtcta
     9001 tattgattac gtaattgtca ttaaggtaaa gactggattt acccagtctt tcagcatctc
     9061 cgcaatcaag ggctattacc gcatcaaagt tttcgaattg accgggcttc cgaacctgct
     9121 cggctccttc caaaaaagca tatatctttg gaatttcatc atcaagaatg aatcttacgt
     9181 cctttccgca tttcttcagg gcattataaa gtgcaaggct ggatccgata ctgtcaccgt
     9241 ccggcattat atgagataat atggcaattt tactgctctc cttgattttg cttattatgt
     9301 tttgaaaaat caaaggttct ctcctccgtc ctttttcttt acctcattca agagcttgtt
     9361 tatgtatgct ccatgttcaa tggatctgtc aagctcaaaa atcacctccg gcaaatatcg
     9421 gagttcaatc ttccttccca attcacgccg gataaatcct gcggcgttgg taaggccctt
     9481 cattgtagcc ttcttttctt catcacttcc aagaatgctt acaaatacct ttgcatatct
     9541 caaatcagcg gttacttctg ctgatactac gctgcacatt gttgatatcc ttgggtcatt
     9601 caggtcgttt ctgattatat cactgactac ccttttcact tcttcggata ttctgtctat
     9661 tctatgatta gccaccgtta atcacctgcc tgtatatttt ggatatgtta ttttctcgga
     9721 actgcctctg tcgtatagga ctcaataatg tccccttctt taatgtcatt aaacttctct
     9781 atgttcaagc cgcattcgta tcctgcattg acttcttttg catcatcctt gaatctcttc
     9841 agggatgcca gctttccttc gtgtattacg atgccgtcgc ggataaccct gatatcgtta
     9901 tttctgttta ttttaccgtc agtgacatag catcctgcta ttgtgcctac tgcagaagct
     9961 ttaaaggtgg cgcggacttc cgcgtgtccc aataacactt ctttatattc gggctccagc
    10021 atacccttca tagcagcttc tacatcctca attgcattgt aaattaccct atacagcctg
    10081 atatcgacct tttccttttc ggcaagggca atggccatag gctgcggcct gacattgaat
    10141 cctataatta tcgcgtttga agcggaagca agcattacat cggattcggt tattgcacct
    10201 acaccgccat gtattgtatt cacttttact tcttcattgc ttaaccttgc aagagattgc
    10261 ctcacagctt ccacagaacc ctgcacatca gcttttagta tgatgttaag ctccttgact
    10321 ttgccttcct gtatctgatt gaagagctcc tcaagagaca ctgtctgctt gctctgtatc
    10381 tgtgaaagac gctgcttttc ctttcgtttt tccgaaatag tctttgctgt cttttcatca
    10441 tccacaacaa tgaatatgtc ccccgcatca ggaacttcat caagcccctg tatttccaca
    10501 ggtgttgaag ggcctgcttt tttgattctc tttcctttat cgtctatcat gccccttact
    10561 tttccgtgag tgcttccaac tatgaagtaa tctccaaggc tcaaggagcc gtcttgaata
    10621 agcacagttg caacagggcc ttttcctttg tccagctctg cttctattac tgtacccttt
    10681 ccttttttgt tgggattggc ttttaattcc tgcatttcgg caacaagcaa tatcatttca
    10741 agaagttccg atatgccttc ccttttcttt gccgaaaccg gaaccattat agtatcgcct
    10801 ccccattcct ctgaaacaag gccgtattca gtcaattcct gctttactct gtcgggattg
    10861 gctccgggtt tgtcaatttt gtttattgct ataatcaatg ctacatctgc cgctttcgca
    10921 tggtttattg cttctaccgt ctgcggcatt acgccgtcat ccgctgcaac gaccaatact
    10981 gcaatatcgg taacctttgc ccccctggcc ctcattgccg taaaggcttc gtggcccgga
    11041 gtgtccagga atgctatttt tctgtcaccg atagttactg tatacgcccc tatatgctgt
    11101 gttattccgc cggcctccgt tgcaattaca ttagtctgtc ttattgcatc cagcaaggac
    11161 gtctttccgt ggtctacatg ccccatgata gttacaacag gagggcgcgg cctcaaatcc
    11221 tctggcctgt cttcttcagt cttgagaagc acttcttcgc tttccttgtc aataacctta
    11281 tctacggtaa aaccgaattc ttcacatatc agctgcgccg tatcaaaatc cagcatttga
    11341 ttaatgtttg caatgatacc caacagcagg agctttttga ttatttctgt cacaggcttt
    11401 ccaagtcttt cgctaagttc cttcagagat atcatatcgc ctattttaac aggcttcttt
    11461 gagacatgga cttcttcttt ttttggtgcc tcaaagcccc gctgcttaaa attcttattg
    11521 tagggctttc cctgctgctg ttgcctgaac ggaggttttc cctgagggga tttgcccggg
    11581 agtttctttc ctattctttc aacttttgta aaatcttttt ttgtgctaag cgttattgca
    11641 tcttcttcta tttcatcatg agcggatacg ttcttgggag cactgaccgg ccttatatca
    11701 gaagacggat ttcttttatc ccctgagggg tgctgctgag gtttacccgc cggcctgatg
    11761 tccttttttt gttccggcct cacaggctgc tgcgcagact gaggcctttg ctgcggctgc
    11821 tgcactgtct gcggcttctg ctgcggtttc tgagcctgcg gaggctgnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tgctgcgcag tctgcggtgc cctctgagcc
    12001 ttctgttcct gttgaggctg ctgtgccgcc tgcggtttct gctgagtttg ctgaggctgc
    12061 tgaggctgct gaggtttctg aggaagagtc ctcttcttgg gctgttgagc ttgaattggc
    12121 tttggggctt caacattatc atctttttta agatgcttct taattgcgga tgcttctgaa
    12181 tcctccagac tgctcatatg gttattaaca tttatattca gctctttggc tgccgcaatc
    12241 aattctttgc tttgaactcc aagttccttt gccaattcgt atattcttat tttattcaat
    12301 aaaaccaccc ccaggttttt ctttttttct cacttcatca ataagcttgg ctttgattcc
    12361 attgctgaag ctttcgtcag ttattcccag taccgatctt gcactctttc ccaaactgat
    12421 gccgaattca tccttcaatc cccatactat aaaaggtata ctgtagaatt ctgctgaatt
    12481 gctcatacgc ttctttgtat tatcagaggc atcctctgaa attattacca gcttgactct
    12541 gccgtttctt attgagtttc tcactgcatc ttcgcctgta accagtttgc ctgctttatt
    12601 ggcaaggctt atcatcgaat atgccttatt catgggaaag ctgcgtcctt aatctattga
    12661 atacatcttc atctaccggt gcttccagtg cattttccac cctctttgcc ttaatggcag
    12721 cttccaggca cgtcaggtcg ggacatatat agcagcctct tcccggtgct tttccaacct
    12781 tatctatatc cacctttcct tcggggcttc ttactatcct gattagttcc cttttaggtt
    12841 tcatttcgtt acagccgaga cattttctca gaggtacttt tctttttctt tgcatagaat
    12901 ctgcctcctt taaataccat aggcctgtga ttgactcttt atgtcaattt tccagcctgt
    12961 aagcttggca gccagccttg cattctgtcc ttccttgccg atggcgagag agagctgtga
    13021 atctcctacg acaactcttg cggcatttgt ttccttgtct gcaaatactt ctattacttt
    13081 tgcagggctt aatgcactgc ctacaaatat ctcaggatca ttgctccact gaatgatgtc
    13141 tattttctct cccttaagct cgtcaactat tacttgaact ctatggcccc tttgccctac
    13201 acaggctccg accgggtcaa tattctcatc gtgggaatat acggcaattt ttgttctgga
    13261 tcctgcttct cttgctatgc tttttatttc tacaattccg ctttgtattt ccggaacctc
    13321 cagctcaaaa agtctcttca ccaaaccggg atgcgttctt gaaagtatta tctgcgggcc
    13381 ttttgtggtc ttttttactt ccacaatata ggtttttatc ctgtcaccta tattatacac
    13441 ttcattcggg gtttgttccc cgggccccaa tatggcttcg gttttgccca ggtcaatgaa
    13501 aacactgttc ttttccttcc tctgcacaag tcctgttact atttcatttt ccctgcttat
    13561 atattcatca aatatgatgc ctctttccgc ctcccgaatc ctctgcacca ccacctgttt
    13621 ggctgtctga gccgcaattc ttccgaaatt cttcggtgtg accttcgttt caactacatt
    13681 tcctgcttca taattcttat ctattgcttt tgcatcctga aggcttattt gcataaattc
    13741 attatcgacc tcttcaacca ctgtcttaag tgcgtaaacg tcaacctctc cggtttccct
    13801 gtttatttga accttgacat tctgtgaggt tccaaagttt ttcttgaagg ccgaaaccag
    13861 cgcaacatca attgcatcta tgagcagttc ctttgaaata cccttttcct ttgctatttg
    13921 ttccagtgcc tggagaaact ctccattcat ttaatgctcc ctcctataaa tatattaaaa
    13981 ctttattaaa ggccgtatta aggcaattga ggccttttct acatttaaag ccttaccttc
    14041 aacttcaatt tctactgaac tttcagtatg ccctttaagg ataccttcaa taatcttctt
    14101 gccctccaag gggtaaaaca gtttaatctc aatcggcttt ccgatattct tcctatagtc
    14161 tctgtccgtc ttcagaggcc tatccaggcc gggagaagag acttcaaata tatatgactg
    14221 ttctatcgga tcggctatat caagcaaatc gctgagttct tcactgacag actggcagtc
    14281 atctatcgtt attcctcctt ctttgtctat gtaaactctg agataccagt acggtccttc
    14341 ttttttgtac tctatttcta caagctcgaa gttgtttctg tcaattatag gctttgccag
    14401 ctcgtagaca atatcttcaa cttttcgttt tgccaaggcg tccactctcc ttttatgtga
    14461 tattctgagt aatttttctt atattaatac gaaagagtgg gatttgccca ctctggtaat
    14521 aaagtcatta ataaatactt tgctgcatac tcgcaggaaa acattgtttc cctatccatt
    14581 acaaaattat agcactcttt tatattacat gcaagtttta tgttatttat actccaaggt
    14641 ttccgcctgc tgagaacagg cttagctggt tgctttcagg aagcccttca aggcatccgt
    14701 gattcttgag tatttctatt actgtcttgg ataaccttgc tctttcccga agatcatcca
    14761 tggatatgaa gcatgattcc tgccttgcgc acgctatatt ctgcgcggcg cttgcgccta
    14821 ctccctgaag ggaagccaat gggggcagaa tcccttcctt tgtaaccata aatcttttta
    14881 catcggattt ataaatatcc actggcaaaa gctttatacc tctgagatac atttcataaa
    14941 caacctctat aagggtcata aggttttttt cctttgcagt tgcattgttc cccaatttat
    15001 cgatttctat ccattttgcc cttatggatt cctttccctt tatgaacaaa tcagcatcga
    15061 aatcctccag ttttactgta taataagtgg catagaaagc ctcgggatag tacaccttgt
    15121 aatatgccac cctgaaggac atcatgacat atgccgtggc atgagctttc ggaaacaggt
    15181 acttgacttt cttgcaggat tctatgtacc attcgggaat attctgttcc ctcataacct
    15241 cttcgtattc gggagtcaaa cctttgcctt tccttacatt ttccgcaatc ttgaaggagg
    15301 ttcttgggtt tactcccttg tatatcaggt acaacattat atcatctctt gtggcaataa
    15361 cttccttaag ggttgcggta ccgcttttta tcagttcctg tgcattgttg agccaaacat
    15421 cggtgccgtg ggaaagccct gaaatcctca ccagctccgc aaaggtgccg ggttgtgtgt
    15481 ccataagcat ctgccgaacg aattttgttc cgaattccgg tattccaagg cttccgacct
    15541 tgcattcaat atctttaagg cttattccca gtgcctccgc agatgtaaac aggctcattg
    15601 tcctggaatc atcaaagggt atattattaa ctccgaagcc tgttatgtcc tcaagcattc
    15661 ttatgattga cggaacatca tggcccagga tatccagctt aagcagcctc ccgcttatgg
    15721 aatgatagtc aaagtgtgtg gttattatat tggagcttgg gtcatcagcc ggcctctgaa
    15781 tgggagtaaa gttatatata tcggtgtaat ccggtactat catgactcct cccgggtgct
    15841 gccccgttgt cctctttatc ccggtacagc cctttatgag tctgttggct tcagcattgt
    15901 ttatttttat tcctttttct tcatagtact tttttatgaa gccgtaagcc gttttttccg
    15961 ctattgttcc gatggtgccc gccctgaaaa cattcccctt cccgaataat ccttcagtgt
    16021 atttatgagc ttcaggctgg tattctccgg caaagttcag gtcaatatca ggttccttgt
    16081 ctccctcaaa acccaggaac atctcaaaag gtatgtcatg accctctttt ctgagtactg
    16141 ttccgcattg ggggcaatta tcgtcattaa ggtcagcccc tgattctatt gatccgtcaa
    16201 ggaaaaaaag ggagtacttg catttcgggc acaaataatg ggggggcaac gggtttacct
    16261 cggtaatatc acacattgta gcaacaaagg aagagcctac ggagcctctt gagcctacca
    16321 ggtaaccgtc cgccattgac tttgcaacaa gcttttgcgc tataaggtac ataacggcgt
    16381 aaccgttgtt tattattgag ttaagttcct tatcaagccg cttttcaaca acctcgggaa
    16441 gtatttcgcc ataaagctca tgggcttttt tcatggtcat ttccctaatc tgctcttctg
    16501 ctccctcgat tttgggcgga tatgtattct ccggtatagg aattatctcc tcaatctgtt
    16561 ccagtattct gttggggttc ctcaccacta ccgaataggc ggtttccctg ccgagatatt
    16621 caaactcctc catcatttca tcggttgttt taaaatgcaa cggtgcctgc tcatcagcat
    16681 cagagtagcc ctggcctgcc atgattatgc gccggtatat ctcatcctcc ggttcaagaa
    16741 aatgaacatc ccctgtagca actatcattt tgttcagctt cttgcccagt tcaacaatcc
    16801 gtctgtttat agcttcaagc tctcctttat ttgcaacaat tccgcttctt acgagaaatt
    16861 cgttgttgcc gttgggttgt atttccagat agtcatagaa tctggcgttt tcttctattg
    16921 tattttcatc caggttgttc agtatattcc tgaacacttc acccgattca caggcggaac
    16981 cgattaacaa gccttcccgg tactgcataa gcaggctttt gggcagccgc ggctttttat
    17041 aaaagaagtc caggtgggac atcgatatca gtttgtacag gttcctcagg cctgtcatat
    17101 tctttaccag gagaattata tgataggatt cacctgcctt atggcttctt cctgacagag
    17161 agagcttgtt tatatcagct gcgtcattta ttcctttgct cttcatcatt tcaataaacc
    17221 tgagcattat ctctgcggtt gctcttgcgt catcggcagc tctgtgatgg ttttccagct
    17281 caatgcccag gtattcgcat acaatatcca gcttatgcct ctttaatccg ccaagcagca
    17341 cccttgacat ctggagggta tcgattattg tattattaaa gattaagccc aattccgccg
    17401 cctttgccct gataaaaccg ctgtcaaagt ttgcattatg cgcaactaac ggtgaatcac
    17461 cagcaaactc aaggaatttc ggcaatattt ccctgattgt gggtttgtct ttaaccattg
    17521 agtccgttat tccggttagt tttgtaatat tttcgggaat cggaatttcg ggattgataa
    17581 gttctgaaaa cctgtccgtt atcctgccct gttttatttt aaccgcgcca atttcggtta
    17641 ttttgtcttt tatgggataa aggccggtag tctcaatatc aaatactgta aattcttctt
    17701 cgaagcttct gtttacagca ttgtaaacta tcggtactcc atcatttaca aagtagcctt
    17761 caactccgta tattaccttg actccatgga tttttgacgc ttcataggcc tccggatatg
    17821 cctgaaccac tccgtggtct gtaattgcaa ttgccttatg cccccaggat tttgcctttt
    17881 taatgagatc cgatgcggaa cagatgccgt ccatggcgct cattcccgta tgggcatgaa
    17941 gctcaactct ttttacttcc gcattatccg tgcgtattga tttttccgct gccgtaatgc
    18001 tgattgggaa tactaccaat tctttaatga acttgtcata ctggatatcc ccttttaagg
    18061 caatatacct gccgtcgatg atgtttgaag tcaatttggc gaaatcattt tcttcctttg
    18121 caaaaatttt ggccgttatt gaatttgtat tgtccgttat attgaaggtt ataattgttc
    18181 tgccgttatt gagtgttttt atttccgtgt tgaatatgca gccctggatt gtaactcttc
    18241 cggaatcctc ggttattgaa gcgatgctga ccggagtgtc tttgaaaggc ttgcccatta
    18301 tcagttcatt cttgttctcc tttgctttgc cgctgttgtc gctttttatg ggcttcaagg
    18361 cttcggttat tattttgttc tcctcttcaa aataacactt cagatattca tcctcgcagt
    18421 acttttcttc attatcggca catattgata catgcaggct gatattgaaa tagtcattaa
    18481 tataccgttc aataatatca ttgcattttt ttgcggtgag gtattttgca gcttcctccg
    18541 attttaccgt gattctgatg acgttatttt caagctgtac atttgaatcc ataataaaac
    18601 ccgtaaaaga cgggttgctt gcaataactg ctttttttaa gtcctcccag tatgatttaa
    18661 caagctcatc tgcgcttaag ctgacagatg actcatcaat gattattcgt accttattca
    18721 gtttaaagta ttctgctatg gctgattcaa tttctccaag ctcgggaata ggaataaagc
    18781 cctttaagct tattatcatt tcccattccc ttgctttttt cagaaccctt atttttttca
    18841 gttcgaactt gtccttatat tgaagaaaaa gattcgagtc cggtttcata tcattttgca
    18901 tattgacctc cgaaggctgc cgcctattta ctgctgcctg attttatatt atagctgctt
    18961 agaatcttta caatatccca gtacattttc aggcgcgcat gcacacccct tataattcca
    19021 agcttttctt cctttgtaac atgagtcatg tctggaaggt ctacctcctg aaccctgaca
    19081 ttgaatttat cgacatattt tgttaaagca acctcaacac catatctggt aatatctata
    19141 ttcggtattt tatcaatgat tgatttctta attgccctct gtcccgaaag gtatggcgtt
    19201 accttttgag ccagatccgt caccattctt cctttcttaa atattcctat tgtcatatct
    19261 gccttgttgt caattaccgg ttctatgaga tccaaaacat gcttttccgt aagacctata
    19321 aggtcggcat ccagaaacaa tattatttct gttccgcatt cgttaatacc tgctctgatt
    19381 gcaccgcctt tgcctacatt ctcatcaagc tcaattacat ccgcaccgca ttctcttgca
    19441 atttccgcag tactgtcggt ggagccgtcg ctgaccacta ttaccttctg aattttgtca
    19501 atgcttttta cacactttac tacgtctgca atcgtctgtt cttcgttata agcaggaatt
    19561 actgctgtta cattcattgc gtttcctcct cttggtactg cgcattattc tgtcatagca
    19621 tgcaaaaatt atacggattg taaagcttct atctccttta tcaattcttc tatgacattt
    19681 tcctgaggta tcttcctgac tgttttgcct ttcaggaata acaggaactc cccttgcccc
    19741 ccggcaatac ccaaatcagc ttcttttgcc tctcccgggc cgtttaccgc acagcccata
    19801 attgcaacct tcataggctt cttagcttct attcctttaa ttcttttttc aacctcttcc
    19861 gtgattctga taagatcaac acaggttctt ccgcaagtgg ggcaggatat tatctctatc
    19921 ccctctttaa gcagcttcag gctcttgagt atctcttttg ccgcatatat ttcctctgcg
    19981 gggtctcctg tcagggaaac ccttatggta tcccctatac cgttgtaaag cagtataccc
    20041 agtccaaccg atgattttac ggaacccgca agcaatgtcc ccgcctcggt tatacccagg
    20101 tgaagagggt agttggcggc cttggatgct tttatatagc tgtctattgt cagcagcaca
    20161 tctgaagatt taatggaaat tactatatca ctaaagtcca tagactcaac aagccttaca
    20221 cttttcaaag cgctttccac cagcgcatcg gctgtggggc ttccgtactt ttcaagaatc
    20281 tctttttcca gggaacctga attaatgccg attctgatgg gaatacccct ttccttggct
    20341 gcataaataa ctttttttac cctttcattg tccccaatat tccccggatt tatccttatt
    20401 ttgtcggcgc cgttgataat tgattgtatt gccagcctat agtcaaaatg aatatcggca
    20461 accaagggta ttgatattct gcttttaata tcttttactg cttctgcggc ttccatatcg
    20521 ggaactgcga ttctaataac atcacagcct gcagcttcca gcctcttaat ctgttctatg
    20581 gtgcccgctt tgtcctgagt cctggtattt gtcatagact gtataaggat tggctctccg
    20641 cctcctatat atctgtcacc tattcttatt ttccttgtat atgtgcccat aatccgccct
    20701 acctgaaaat atccattaaa ttgaatctta taatatccct gtacataagg aatattgaaa
    20761 gagccatcag tgctataagg cctacaaaat gcaccatacc ttccttttcg gcaggaaggg
    20821 gttttcttct gataagctca ataaacagga ataacagcct tcctccgtca agggcgggca
    20881 atggaagcag gttaattata ccaaggttca ggcttattaa ggctgtaagg taaagcactt
    20941 ttatgaaacc tgccttggca gcttcattta tgtatacaag tattgaaatg ggcccgctca
    21001 cttcatttgc atcagctttg cctacaacca gctgtcccag aaaactcagc atcatttttg
    21061 atacggcaaa agtcgtattt attccttctt taatggaaaa tcccgaaatg ccaaccttct
    21121 gagtgatgcc tattacaata tcatccgtgc cggggttctt aaccggagta agcattactt
    21181 ccctttgaac tccgtttttc tcaaccgtta cggttatctg ttcgccttca tgctttgcta
    21241 tttcaggttt catttctttc cattcattta tctttacgtc atttataccg actatcattt
    21301 ctccggcctt aatacctgac gtttcagcag gcattccttt tgtcacctgc ccgactatcg
    21361 gagcagccac cggggcaatt accagtgaat atatgagtat tgtcaaaatg atattgaaaa
    21421 ccggaccgga tataacagaa gatatttttg aaattatagg gctgttatta aaggcccccg
    21481 gttcattatt ttcttcatcc tctcccagca tcaggcaaaa tccccctata ggaaatgccc
    21541 ttagtgaata cactgtttct ccgaattttt tgccgataat tttcggcccc atacccagtg
    21601 cgaattctac aacctttatg ccgccgagac gtgcggcggc gaaatgcccc gcctcatgta
    21661 caaatactat taaattaaat acaagtatcg cagcaattaa cgtcattact tacaccccgt
    21721 ttcatacaaa taggccagtg tctctcttga ccatcggtca agctctgaga cttcttctat
    21781 atccatattc tcaattattt tatgtttatc aagagtcttt tctataaggt ttcctatatc
    21841 aaggaagctg attttgcccg agaggaataa ttttaccgct tcttcattgg cgccgtttaa
    21901 taccacacag gcgctgtctc ccatatccag agccctgaat gccagcctca ggcatttgaa
    21961 tttctccgta tccggcttgt aaaaggtcaa ctctccaact gaggtcaggc tgagttttgc
    22021 actcttcatc ggcagccttt ccggatatgt cagggcgtat tgaatgggaa ctttcatatc
    22081 gggcagtccc agttgtgcaa taactgcgcc gtccacaaat tctaccgccg aatggataat
    22141 gctttgggga tgcacgcata cttctattga ttcgggcatt ataccaaaca gccatctggc
    22201 ttctatgact tcaagcccct tgttcatgag agttgctgaa tctattgtta ttttcctgcc
    22261 catggaccaa ttgggatgct tcaatgcatc ctgcacagta acgctctcca attccttcag
    22321 ggtcttgttc ctgaaaggcc cccctgaagc tgtaagtatt atttttgata tttcattatg
    22381 cctgctgccg gcaaggcatt gaaatattgc agaatgctca ctgtcaaccg gaagcatgtt
    22441 cactttgttc tttttaactt cccgggttat aatcctgcct gcggtaacaa gggtttcctt
    22501 atttgcaagt gctatattct tacctgtttt tatggcatta tatgtaggaa tgagccctgc
    22561 tataccaaca atcgccgaaa ctaaggtatc gcagctttcc aaagttgctg cttccaccac
    22621 tccttcaagg cctgcggcaa cacaagtcat tcctcccaca cggcttttaa attctttgta
    22681 ttttgacatg tctcctatgg cagcaatctt tggcctgaat tcccttacct gggcttccag
    22741 caagtcaata ttcgaattgg ctgccagtgc atctattttg aattcttcct tattttctct
    22801 tataatatcc aaggtctgtg tgcctattga gcctgtagac cccagaactg ataatacttt
    22861 cactatatca aatccccaat cagaacgaaa taataataaa tgatcggtgc ggtaaagaga
    22921 atgctgtcga acctgtccag tattccgccg tgccccggaa ttatgtttcc aaaatccttg
    22981 actcctgcat atcttttaat atatgaggca gccaagtcac ctgtctctga agcaataccg
    23041 cagagaaaac ctgttattat aaaatgaatc agccttattt caattccgta attatttaat
    23101 ataatacctg tgattatact gaatgcaaca gaacccgcaa ttccgcccag agcaccttcc
    23161 acagtcttct ttgggctgat cgcggggcac aattttctcc tgcccatata tatgcctatg
    23221 aaatatgcga aggtatcggt aaaccatgca gtcaggaaag gaagccacaa tattatattg
    23281 ccgtacctga ggttttcgat gaaaagcagg tggctgaaaa gaaaaggtat atatgctatt
    23341 ccgaaaatgt ttatcgatat ctcagtaaaa ctgtgtttct ggcttatgat ttcatatata
    23401 agcaatgtga tgacagaaag cgcaatcaaa aacccgggcc tgttaaaggc aattggaaga
    23461 agaaacataa tatagtacaa aataactata gtatatccga atattttgtt tgtgcttata
    23521 tttatgttgc tcagtgccct gtaatattca aataatcctg tcaaagagac tgccgctgta
    23581 aacaggtaaa gatacagccc tcctgaatac aatataaata ttataagcgg tataccgagc
    23641 agcgcactta atacccttgt tttcagcatt tttacctcca ctacttcaat gcaccaaaac
    23701 gcctgctcct gtttttatat tccaatatag cactgtgaag caccgcctca tcaaagtccg
    23761 gccagagttg gtctacaaaa agcagttcac tgtatgcccc ctgccatagc aggaaattac
    23821 ttatacgctg ttcgccgctt gttcgtatta tcaagtcggg atcaggatca ttgcttgtgt
    23881 acaaataatt tgaaaacact ttttcgtcag cggaatccat gtcaagattt ccggcctcca
    23941 agtccttaat aaggtttctg caggctctca gtatttctgc ccggcctccg taattcagcg
    24001 ctatgttgaa ttgaagccct tcattattct ttgtaagctc gatagcctct tctatctcag
    24061 ttctcgtttg gtcaggaagc atatcaacct cacccaatat ttttatcttt actttattct
    24121 tatggagcgc attcacttct cttctcatat attccaccag cagatccata agatagttca
    24181 cttcatcctg cggccggttc cagttttctg tcgaaaaggc atatacggtc aatatcttaa
    24241 ttcccaagtc gctgcagctc ttaaccgtct tctttaaagc ttccatgcct gctttatgcc
    24301 ccattactct tggcaagcct tttttttgtg cccagcggcc gtttccgtcc attattatgg
    24361 ctatatgtac aggaagcttg cccttatcaa tatctccaag catcagagaa cctcctcaaa
    24421 aaagtatccc tcatggtgag ggaaactatt taaaacacga tgtcgtaagt attattttgt
    24481 cacactcagc cttctgcctg acaacaatag gtgaatttcc ccgtctttct ttctttttat
    24541 cttcgaaggg tgttgatgtt gaatcaataa tgatatcatg tttttcaccg attatctgca
    24601 gtgcatgatc cagcgtatat cctattacca gaggtatatt gtccaatatg ctctaaacct
    24661 ccaaaatttc tttttccttc gcttctatag ctttatcagc ttctttgata aacttgtccg
    24721 ttattttctg tatgcttccc tctgattttt tgaggtcgtc ttcagtaatg tctccgtcct
    24781 tcttaagctt cttaagatgt tcatttgcat cccgccgtat attcctaatt gctacctttg
    24841 attcttcacc aagcttttta accactttca ccagttcttt tcttctttcc tctgtaagct
    24901 gcggtattac aagccttatc atcttaccgt cattggaggg gttaataccc agatcggatt
    24961 ttagtatggc tttttcaatg acgggaatag attttgcatc ccacggactt atagtaagaa
    25021 ctctgggttc cggtgcagca atgtttgcca gttgattcaa aggtgttggg gctccatagt
    25081 attcaacagt aattctgtcc agaagacttg gatttgctcg tcccgcttta agggtagaaa
    25141 gttcatgctt aaacacggtg agggtctttt ccattttctc ctgaatgttc ttttcgatat
    25201 cctttatcat accgttaatc ctcctttact actgtaccta tcttttcccc cataattgcc
    25261 ttaattatat tatcggggtt tttcaagtca aatacaagta tgggaatttt attatcacgg
    25321 cataaggatg cagccgtgct atccattacg cccaatcctt tgttgattac ttccatatat
    25381 gaaagcttgt catattttct tgcttcttta tttataacgg ggtcactgtc atatacacca
    25441 tcaacttttt ttgccataag gattatatcc gcttctattt ccgctgccct tagagcggca
    25501 gtagtatcag tggagaagta agcgttgccg attccggatg caaatattac tacccttccc
    25561 ttttcaaggt gcctgactgc tcttcttctg atatagggtt cagctatctg tctcatctca
    25621 attgcagtct gaacgcgggt gggcacattt attccttcca atgcatcctg aagtgcaaga
    25681 gcgtttatga cagtcgcaag cattcccata tgatcggctg ttgttctgtc cattccctca
    25741 ctggttctgc ccctccagaa attgcctccg ccgactacta tcgctacttc cacattaagc
    25801 tcccttaaca gcttgactct ttccgcaacg tctaggagca tgtcatgatc caatccgaat
    25861 gatttttctc ccgccaaagc ttcaccgctt attttaagta tcactctctt atatttcacg
    25921 tcactcatat atattatacc tccaagtatt ttgcaacacg gcatgtataa gtataaaatt
    25981 tctacaaatc caataaaaat ccttctgttt ttcaaaaaaa agagaacact aagtgttccc
    26041 tatttattca cctgactcat tacttcatca acgaagttgt cttctttctt ctcaaggcct
    26101 tcgcctcttt caaacctgac aaaccttctt atcgtaatat tttctccgat ttttgctatc
    26161 agtgatgtaa gtatttcctg cactgtcttg tctgtatcct taatgaaggg ctgttccaga
    26221 aggcatattt ccttgtagaa cttctctatt ctgccttcga ccatcctttc aataattttt
    26281 tcaggttttc cctcattcaa agcctgggct ttgtatattt cacgctcttt ctctataacc
    26341 ttgggatcaa catcttccct tttgacgtat aaaggattgg aagcagcaac ctgcattgcc
    26401 atgtccttta caaactgtct gaattcttcg ttggctgcaa caaagtctgt ttcggaattt
    26461 acttcaatta gtactccgat tcttccgcct ccgtgtatgt atgagtcaac tattccttcg
    26521 cttgctattc tgcctgcttt ttttgcggca gcggcaagcc ctttttctct tagtatttct
    26581 attgctttct ccatgtctcc gttgctttcg gtaagagctt ttttacagtc catcatgcct
    26641 gaaccggttc tttctcttaa ttccttaaca gctgaagctg aaatcatttt cttcgcctcc
    26701 tatgatattt atgaaaaagg cggctgtcgc cgccttatat acttaccaag cagttactca
    26761 gcaagctgtt caccttgtct agcttcaata acagcatctg caatttttga agtaagaagt
    26821 tttacggccc taatagcatc gtcgttgccg ggaatcggat agtctacttc ttcaggatca
    26881 caattggtat caactattgc aactatcgga atcccgagaa tgtgcgcttc ctgaattgca
    26941 attttttctt ttctggggtc aaccacaaaa atcgctcccg gattctcacc catgttcttg
    27001 ataccgccaa gattcttctg aagcttttcc ttttcagctc tcagattgat aacttccttc
    27061 tttggaagta cttcaaaagt gccgtcttct tccatttttt ccagttcatt aagtctttca
    27121 attctttttc tgatagtctt gaagttggtt agcatcccgc ctagccatct ttggttcaca
    27181 tagaacatat tggctcttat tgcttcagat tcgattgact cctgtgcctg cttcttagtt
    27241 cctatgaaaa gtatatcctt gcctgaagct gccacttctt ttatgaagtt gtacgcttct
    27301 tcgaccttct ttactgtttt ctgaagatcg atgatgtata tgccgtttct ttcagtaaag
    27361 atatactccg ccatcttcgg attccatctt cttgtctggt ggccgaagtg cactcctgct
    27421 tccaatagct gtttcattga aattactgac ataatgtttc acctcctata gtttttcctc
    27481 catgaccgtc atatcctttg agaactttgt aaaagcactc ccccgaagga tcaggccatg
    27541 tgtgtttttc accttaaaca gtttatcata taacatattt attggcaata atttttttaa
    27601 atcggtgtct gatatctcgt atttattcta tctaattata caaaatttat gtaatgtcat
    27661 tttgtaagta tgcttattat ttcatcataa ccggagcctt tgggtatctc aaaagtgcct
    27721 tccttaagct ttgtctcatt atttgtctct ttaagcttat caatgaaagc tcccgaatca
    27781 ttaataatac ccttctgccg gagaagagcg gctatactct cggaactcat accgcttttg
    27841 atttctaccg tgaccttctc cgttggagca gtattctcct gattccggct gtctataatc
    27901 tcctcttttg aaaaaacaac aaggccgtgt ttttgcgcct ctttcttaat ttcttccaat
    27961 gtcagttcct tgtttcccat gcttatattc atcattgatg caagtacaag gcctattccc
    28021 aaaccaagta ttatattttt caggttatag ttccccatta ttctacctca tttttcaagg
    28081 acaatataag cataacctct ccttgcccta tattcatctg ctttgcaata tcggtaacac
    28141 tcatgccctg agctgcatat tcaaaaacct ttaactgctt ttgactgagc ttttcatcaa
    28201 caggcttatc caaatcagaa ttgtcattga ttatcttttt ctttttattt gccgcgctga
    28261 catttggtac gacaggggca gcaggcttat tttctgccga tgaggtgccg ggaacctgat
    28321 tatttaggct cttcaattct tccctgaagc tgcttaattt ctcttcaatt tcctgcacta
    28381 ccatttctga tgtgtaattt agctcggata ttatttcatc agcatcttca ataaccttct
    28441 ttatctcctg ctctttaacg tccatgctga aatatatatc ttcgcccttt actttatcaa
    28501 gaaaaatcat tattgaagca gccagtatga caaatactcc aatagccatt aatacatagt
    28561 taatcatctg agcacctcgt tcatatcctt atatctattg ttgacatggg gtccttgttt
    28621 ttcttatttt cctggccttc attatcttca ggcctgctgt ccttatgttc gcccttctga
    28681 tccccttgcc ctctgttttg cttcccatcc ctgttaatcc tggcttcctg tgcctttttc
    28741 gtttcaatta tctgctgtgt gtttttatct tgctgctgtt gttctttgtg catattaata
    28801 ctgagattat tcttttccag ttcgttttct gcgttttttg ccttctgtac ctcaggcagc
    28861 tttggtataa ttgtttgtaa gtcaacaggc cgcaaagaca ttataatcac tcccaattat
    28921 ttatcatata tcattatagg aaagcatttt tatctcagca tgcattctgc aaaaggaaac
    28981 aaactttata gaatccttga tgtacatcat gctgcttccg attattatcc tgctgccggg
    29041 ataaataata tcatatacct ttacttttcc ttttgataat tcttcgaaat aaacctccaa
    29101 ttcattctgc tcgtttaata actgatcttg cttctgctta agctgcagct taagctgtaa
    29161 ggattttgcc tgaagtgcct ttttatcctc cggcagttcc atacttttgc tcatttttgt
    29221 cagaagatca accgccttgc ttgttttgtc caggttattc gtaacctgct tcaattcatt
    29281 ctttaattct tcgaatttat ttctcatttc agggttgacg ccgacttcca gttctgtaat
    29341 cgtagccatc ggagaaccta caacctttgc tcttatttct tcgcctacct taaaagtacc
    29401 gccaaccagc aagccttttc tgccggttgc ctccagcttc tttccgcagg taacctggct
    29461 gtgcattatt gcatcgctct ggatgtttcc ttttacatct atcttgctgt tttctataaa
    29521 cttggctaca agatcccctt cgcagtaaag ctctcctctg ttcatacctt gtattcctct
    29581 gtgaagtatt atattgcctt tggattttat ctgagcccct tcaacaacac cataaacttc
    29641 cacatctcct tcggcctcga ttaaaaagcc tgtcagaaca ttgcccatta ctattacttt
    29701 tccgttaaac cttatgttgc ctgtagagtt atccacatta ttctttactt cataaacgga
    29761 gaaaacattt accttcccat caatgatttt tacttcaccg tcaattgctg cgacaagctc
    29821 aagttcgtct tcgcttacaa caacattctt tccccttggc aaggcaagct tctttccatc
    29881 ctttccggga acatcttcat cggaaacatt ctttccgggt atgccttcgg tatgcggtat
    29941 aagggttacc agcttctctc cggctttgac attttttata agggacagtt catggtagtc
    30001 aatttttccg tctttgtcta tacctattga cttttcggcg gaagtgtcaa aatggtatac
    30061 aagctccgca tccttccccg gctgcggttc tattccttca gccacaagta tttctttgtc
    30121 atataccttt tgagaaaccg ccttttcaat cacatcttct ttaatgttga attttacacc
    30181 ttcctgctta agtgcttcat ttattacttc taatgtaata tctttgccgc cgatgggttt
    30241 gctgataaca ataaaagcct tcattttgtc cttagccgtt tttacgaggc attcactgtc
    30301 tatcttcttt tccatctggg gctcggctat ttttatccaa ttatcaaggc ttccgatttc
    30361 cagcatttgt tttatttttg taagatccac gctctgaatt cttcgcaggt caatttttgt
    30421 tacaatatcc actattgtaa catctttatc cgctttaata tatactccgt cttctctgaa
    30481 atcaaaatct atacttttgg tgtttgcttt cagattctca ttcagaatac tgtcaatatt
    30541 gctctcaatt atctccaagg ttttattttc ccccttcgct atcttcactt tgtagtaaga
    30601 ggaaaacagg ttctttttgc tttccagaat ctcaatattg agttcttcct tcctggcatt
    30661 aaaatgctcc attccttttg ctattaactt ttcatagtcc ttgccctcga gtattatgta
    30721 ctcattgctc attattatca ccatcccatc attcaaatgc aacttttaac ttgttcttaa
    30781 gccgaatgat tgcttttgta tgaagttgtg aaactcttga ttccgaaata tacaacagct
    30841 ttcctatctc tttatatgta agctcgtcat agtagtatag tgatactacc tttttttctt
    30901 tttctggtaa agcgcttatt gcatctgcaa gtatctcttt catttcctta tcttcaatca
    30961 cattttcagg agtttcggaa tgcttgtccg cttcattgct gataatttct cttttctgtt
    31021 ccagcaaatc atccagggat actacagaat aagtggtgat attctgtatc ttcttttgga
    31081 attcttccac cgataatcca aagctttccg ccatctcctc atcggtagca ggcctgccaa
    31141 gcctgctttc taagtcattg tatgatttct ccagttcttt ggatttttgt cttatcgaac
    31201 ggggaagcca gtctatttcc ctcaagtgat ctataataga acctcttatt ctaagctgtg
    31261 cataagtttc aaatttaact cctcttgcaa tatcatattt gtcaattgca tctattaatc
    31321 caaatatacc atagctgaca aggtcatcaa attctacatt gtttccataa cttgaatata
    31381 gtcttcccgc aacatattta actatatatg tatatttaag tattaattcc tctctaattt
    31441 ccgcagcttt tgtcttggaa tatacttccc aaagattagc catttcggtt attacctccg
    31501 tgcagtagta taagttaatc cgtattgcta ttgatgtatt tgctcaaatc ggcaggatta
    31561 acttcaataa attcattgtc actgtcataa tcaattgctt ccagtgcttc atcatccaaa
    31621 ggaggcactt ttatgtccag tttacttccc gttgccgata ccgttccgcc tttatcctgg
    31681 atatgtatgc tcactctgta atattcaata agattcaaaa cagtttttgt tatcaatgaa
    31741 ccaaatatac caaatactat ggtaacagcc atacatctta tcatcagttc ggaaaaatct
    31801 atattgttgc atatacctag tgttacagta acaataagtg cggttataac tattaaaatt
    31861 ggtatgaaat cagatattct catattgaaa ccccacattc acaataccgg acatatctaa
    31921 atctgcttcg ttccatagcc tatggttttt attaataata taccattttc tgaatataat
    31981 tcaatagtac gcccatagtt ccctccggtg tcctccgaaa taagcggaat attcaacatt
    32041 ttaagagtat cttttgtagc tgtgacattt cttaaaccta ttctcattac ttcactggca
    32101 tcattgaaat tgaacatctg cgcccctccg gctatcttac tgacaagctt gttccgtttt
    32161 gcaccgattt ttatcatttc tgtcactaat tttacaatac ctgtatctgc gaatttcgct
    32221 tcattgctgt tgttctttat ctgagcgctg ctggggagca taatatgcac catccctgca
    32281 atctttgttg aagggtcata caggcagatt cctacacaag aaccaagccc cagggtagta
    32341 agcactccgg gacaaaatgt cgtattcaaa tctgccatcc caaccttaac tatatcgatc
    32401 atattaaaat actcccaagc tcttaagaag aatctcaaaa gaatcctcgt caggtattaa
    32461 aaagaaattg ctctttacat ggtatgtacc ttcaaataag tcagtttcaa taagcattac
    32521 cctgtcgccg acctgtccga aaagtataac gggtacgctt attattgctc ctgccatatc
    32581 tattgccaga gaaggcacag acatagtcag gttcaaattt gtcaaagttg acaatgagga
    32641 cagataggaa cccgaaagta tgttccccac ttcctgcaat gctgataaat ccatttcatc
    32701 caggtcatct ccgttgctat ctctgcccat aagcaagttt gcaagcattt ttgccgattt
    32761 tttgtcaacc agaaccataa tgctcccgtt aatatcccca tttactttaa aataaatgcc
    32821 gactacctcc gtctcgggcc ctccgactaa ctccgcaaca tttttaaact caaggatatt
    32881 taccttggga acgtccatat caattctctt tgatatcatt tttgacaagg ctgttgctgc
    32941 atttccggca ccgatgtttc cgatttcctt taaaacatca atttgcaggc tattaagttc
    33001 ttcaattgat attcccattt tactcaccct ttttaaacag ccggattagt gattattttc
    33061 actatatcca gcaatatgat aagcctctca cccactttac ctataccgga tatataacct
    33121 tgatctacgc tgccgacacc atccggtgct tcttctatcg catcgctgct tatctcaacc
    33181 acttcggatg aataatcggc tgtaaaccct acgataatat catccactga tactattatt
    33241 atacgggtat tgttgctgtc ttttccttcg ggcaggttga atcttttgcg caggtcaatt
    33301 accggaatca cttctcctct taggttaata acgcctctca cataatgagc ggtattcgga
    33361 actcttgtaa tactcatcat ttctttgatt tcttttaccc tcagtatgtc aattccatat
    33421 tcttcattct ccaatttgaa tattacaaat tgccgtgcaa atgttgctgt ttccaaaaga
    33481 aacacctcct tatatcagtg aatttgaatc aattatcagt gcaacctgtc catccccaag
    33541 tatagttgcc cctgctatat ttctaatgcc tgacaagaac tttccaagag tttttatgac
    33601 tatttcctgc tgccccataa ggctgtccac aataaatgca gcattctttt cgcctttttt
    33661 tacaactacg gcagtcaatt gatcgctgtc atcactggat gcatttataa caccaagcat
    33721 ctttgcaatc cttactatcg gcagaacatt gcccctgttt aatactacat cctgtccatg
    33781 tacatttctg attgtagatg cttcaatatc aattatatcc cttattacat tgagaggtat
    33841 tgcaaatttc tcttcaccga tatttacaag cagtgcctgt ataattgcaa gtgttaaagg
    33901 caatctgata ataaatttgc ttccttttcc cctatagttc tcaacctcaa ccaatccgtt
    33961 aagagattcg atttttgtct tgacaacatc cagtcccact cctctgccgg aaaggcctga
    34021 aaccttgtct gcagtactga agccgggatt gaaaagcagt tcaattgcac ttttctcatc
    34081 caggtcatct gcttcactgc ttgtcaaaat gcctctttct actgcctttc gttttatctt
    34141 ttcaatgtct attcctgctc catcgtcttc cacttctata actacgctgt tgccgtcctg
    34201 atatgccctt aggttgactg ttccgttagc cggcttgtta tttttaatcc tttcttcttt
    34261 ttcttcaatt ccatgatcaa tggagtttct tattaagtgt ataagcgggt cgcctatttc
    34321 gtctatgaca gtcctgtcca gttctgtatc ttcacccgac atgttaagga taatatcctt
    34381 ggaaaggctc ttcgcaaggt ctcttaccat tctggggaac ctgttgaaca ctctttctat
    34441 gggaaccatt cttactttca ttactgcatc atgcaggttt gtagtaatcc tttcaaggta
    34501 ttccactgct tctatcatat cctgattctt tgaatcagtt ccaactccct caagtctggt
    34561 ttttataatt atcagttcgc ttaccaggtt cataagctta tccagcctgt cgatatccac
    34621 ccttactgtc tttcccattc tggccttttt gccttgtatg taatctccgt tttctttgtc
    34681 ttcaattgag tactttaatg tctccttttc ttcattagta atctcatcga tatctttaac
    34741 gtctagttct tcaggaactt caatcctttc aaatatgact tcggttactt cggaaatgga
    34801 ttcaagctct ttcctgatca aagcctcagt ttctttggta attattgcaa tggtaaattc
    34861 attatcgaat ttttcgtctt caatatcttc aaccttcggc tctgacttga aaatctccgc
    34921 atatctctca actgtctgaa ataccagaaa agctcttgct gatttcagca tgcagtcttt
    34981 ggccagcacc accgtaattt tatatactcc atggttctgc tgcacagcct tcctgataat
    35041 attatcatca tattgattaa acttcatttt gctttcctgt ggttctttct tatcggctgt
    35101 gtcagatgct tctgcttcag gctttttcgc cagcatacca ttaaggcctg cgataataga
    35161 atttacatta ctgctgcttt cttttcctgt agcggatatg gtatttatgg aattactaag
    35221 ataatcaagg cattcaaaca gaatgtcaat catagaggaa ttcacttcaa tattaccggt
    35281 tcttattgca tccagaacgt tttccatctg atgagtcaga ttagcaatcc ctgtaaatcc
    35341 cattgtaccg gccataccct ttaatgtatg agcaactctg aagatctcat taagtgtttt
    35401 catatcgcca ggatttgttt ccaggctcaa caggttttga ttcaattcct gcaagtgttc
    35461 caaggattct tcaataaata tttccatgta ctgattcacg tccaaagtat tacaccccca
    35521 tgcattttaa tatttctccc gtaatcccgt gtaaaggagc tatagtatcg atagcgcctg
    35581 tattaactgc tgattttggc attccgtaaa ccacgcaggt tctttcatcc tgtgcaattg
    35641 tttttcctcc tgcattcttt atttccaaaa tcccttcact tccgtcactt cccatacctg
    35701 tcataatgac cgcaataatt ttgtcatgcc ctgtttctgc cactgatttc atcataacat
    35761 ttactgaagg cctgagtcca ttttccggtg cctcactgtt cagaaggatt ttgtacttat
    35821 tatcctcatg gctttttaca agcatatgat agttacccgg agctacataa gcccaacctg
    35881 acgttagtac atcaccgtcc tccgcctctt ttacatttat ctccgacaag ctatccaacc
    35941 tgtttgcaag ggattttgta aagcccggag gcatatgctg tactataagt actgcggcag
    36001 gtatatcctc aggaagcttt gtcacgacct cctggagcgc tttgggccct cctgtggaag
    36061 tacctatggc tacaatgtat ttcaggtatt catcggatct gcttttatgt ttaaccttct
    36121 tgtaaacaga aggcggcttg tattctggtt ttatctgagg gaaatacccc gtctttgacc
    36181 agcatatgga ttttatttta ttgattaact cttccctcat ctgttccgca tcaattgtta
    36241 ttaatgatcg aaccggtttt acaacataat caagagcacc caattcaaat gctttaatcg
    36301 cagcttctcc gccttcatag ctaaggctgg acagtattat aacctttggt acaggcttaa
    36361 gcttcataag ctcaacaaga gcctcaagcc cattcattac cggcatttca atatcaagcg
    36421 tgataatatc cggcttcaat agtattgctt tttccaaccc ttctttgccg tttcgtgctg
    36481 tacctgctac ttcaatatca gcatcgtttt taagcatgtc ggaaatcaat actctcataa
    36541 atgccgaatc gtctacgata agaactctta tcttttcttt cccggaaagc atatcaaatt
    36601 aacccctttt taatcaatct gcgctgttct tcaaatataa acttagtaat tgcattccta
    36661 tccgccatat ttagatttgt atactgcact ccgtaggtat agagaagcct gtagctctcg
    36721 ttaaggattc ggcgtacaat tactccctgc acaaccatta ttggtccgtt aaggtcgagg
    36781 gcaaactcca atgtatcatt ttcattaaaa tattctcttg aactaaacat aagccctcct
    36841 gcacttatgt cctggatagt gcatttaaat ttttcaccat actgtttctc ttcagggtta
    36901 atcaccagcc tggcctctat ttctctgaat attttcagcc tgtaatagtc tcttctctgt
    36961 tttttgatta cttcagaagc tgccttgagc ctgagaagcg gaatacttcc aaaaagcttt
    37021 ttaaacaata tggttctgat ttcataaatt gcttctccct taaatacgat aagcttcaga
    37081 acagtgccct gcctgatatt cacatccatg ttcttatata tcggtatcag tacatcaaaa
    37141 aattcttcat actggccgtc atgaatatca actatctggc tcacgtattg ccttaattcc
    37201 cctactgcat caaaatattc tattagtatt ttatctccta tattgaaata tttcgttaag
    37261 cccttcataa taattctcct tatgaattat aagtgcaaat agatgatatg taaattcatt
    37321 gctgcaattg atatggcaga ttaatcagtt gaataagctg gtaagtttgt ttataaacat
    37381 tttaattcct gaaacagagc tgggactcaa ttctttatta tccatcaaag ttaaagcaat
    37441 atcattcatg ttttttgatg cctcgcttct ggcaaagctt attacaaatg gctgctgaat
    37501 tttgactgcc tttgttacac aagcatcatt cagtatatag ccgagttttt gcaattttac
    37561 cccaaggaac ttctgagtta caatatttaa tttgtcaagg atgttgaaag cctcagccgc
    37621 attgtcagct ctatttacca caacttttat attttttgtt ttatccttgt gggtaatggt
    37681 ttttatcaga gcataagcat ccgttatgga ggtgggctcc ggcgtagtta ccaatatgac
    37741 ctcccgtgca gcatgcacaa agcttataac gttttctgaa agccctgctc ccgtatcaat
    37801 taatatgata tctgcttcct catccagcct tccgagcttc atcagcaaat ccaccagctg
    37861 agttctgttc agtttcacca actcttgaac tccggaacct cccgaaacga attttactcc
    37921 ttcataacct tcttcaagta tttcaagtat gttcttattg ctgtttatta catcggacaa
    37981 attatatttg gacattttgc ccattacaac atccacattg gcaaggccca ggtccgcatc
    38041 gattattacc accctaaggc ccatctgagc aaattttata ccgaggttca cagtaaagtt
    38101 tgtctttcct acaccgcctt ttccgcttgt tactgcaatc actcttgaac cgggtttatc
    38161 cgtaagtatg cctgtccttt tctcttcgtc tttataattc tttatgctgc ttattatttc
    38221 tcttaatctc tcagcttgat ctttcatata gcttatctcc caacaaacag tttattatct
    38281 tgccgttatc ggcaatttca atgtcatcag gcacactttg tccggtggtg atatatgaaa
    38341 tcggcttctt agtgatataa gcaacattta gtatcactcc gaaggctgag gtttcatcaa
    38401 gttttgtaaa aagcagctta tagtcatcca gaaaactgta attctttatt atttctatgc
    38461 aatcggaaaa cttggtggtt gcactgatta ccaaatatac ttcatagttg atgcttgatt
    38521 ttataagaga ttttaattcc ataagctgat atttgtcctt atggctcctg cctgcagtat
    38581 caacaagtat cagatccttc tcggcatagc tttctatggc attaagaatc tctcccggag
    38641 tgtatattat ggataggggt actccgatta tttcactgta ggttttaagc tgctcaaccg
    38701 ctgctattct ataagtatcc gaagtaataa gccctactct ttttttctct gtcagaacca
    38761 tattggctgc cagctttgca agagtagttg ttttacctac acctgtaggg cctatgacta
    38821 taatgacttt tttccctccg gcaaatgaaa agggttccgg ttctttaacg tatttcatca
    38881 aaatattata gacagtattt cttacaattg cgtcgctttg ctgtttttca gagtccaaag
    38941 tctccataag cttctcttta atctcattta taattacctt gtcgacctca ttgtcctcaa
    39001 gggttttaag atacttcttc actacatccg ataattttgt attctcataa tccaccttta
    39061 cggcgtcata aaccttgttc agcatgtttt tgagttccat gaagtcctgt tgtataagct
    39121 ctttatcatc gagatgagtg tgtttttttg tattgtcctt taatgaattc ttctggacct
    39181 gcagattctc attaagttct tttataaatt catttgtttc aatggtatga tcattcctgg
    39241 cattatttgt cctagcccct gaagagatgt cattatctat tgttgcaact acttcaataa
    39301 gaggctttga aaacaaacct gccaggcctt tttttctgat cttccttgtg tttagtatta
    39361 ttgcgtctct tcccaagtcg gattttactt tctgcattgc ttcctgggct gtttcaccaa
    39421 catatctttt tactttcatt atatactcac cacccctacg gattgtatct caaccaatgg
    39481 gtccaattca ttatatgaca gcactattag tcccggagca acctgttcgg tcagtctctt
    39541 gaagtacagt cttacaacag gcgaagccag tattatgggc tgttctccca atgatgtcag
    39601 cttctgcatc tccttaagaa gattgttgat tatccgctgt gctgtatttg gttcgaggtt
    39661 taaatatgaa ccgtactctg tctgctgaac tgaatccatt atggtttgtt caagaaccgc
    39721 atccaaggtt attaccttgg cctgcatttc aactataaag cgtttggtaa tatttcttct
    39781 cattgactgc cttacgtatt cggtaagcat gtcggtgtct tttgtcattg gtgaataatc
    39841 cgcaagggtc tccataatgg taaccatatc ccttatggat acgccttcct tcagcaggtt
    39901 ggcaagaacc ttctgaatat cgccaatgct aagctgcttg ggaataacat cttccaccag
    39961 tgccggatag ttttctctca cattatcaat aattttttgt acatcctgtc tgcccaatag
    40021 ttcatgggca tgattcctta tgatttctgt aaggtgtgtg gcaattactg aaggcggatc
    40081 caccactgta tatcccatca tctctgcccg ctccttctgg ttttcgctga tccacaccgc
    40141 cggcaggcca aaagccggtt ccgtggtctt tactccatcc aattcaccgt cagcagtacc
    40201 gggattcatt gccagataat tgtcgaacat cagttctccg cttgccactt cattaccttt
    40261 aatttttatt acataatcat tgggtttaag ttgtatattg tctctcagcc ttatgaccgg
    40321 cactatcaaa cccaagtcaa gagcacactg ccttcttatc attacaactc tgtctaaaag
    40381 gtctccgccc tggttcacat ccgccaaggg tataatcccg tatccgaact caagttctat
    40441 cgggtccacc tgcagcagcg acataacatt ctcaggtttt ctgatctctt cgacttcaac
    40501 atcctgggct ttgatttcct cttccttttg tgctgctttt atagtctttc tgagggtgta
    40561 ccccatatac gccagtatgg atgccagcaa aatattcgga acaggaggca atcctgtgaa
    40621 tgccagtata agcaatgccc ctgatgcgat ataaagtact tttggttctg ataaaagctg
    40681 tgctatgata tcagttccca ggtctgaatt tgaagccgcc cttgttacaa ccatgcctgc
    40741 cgcaattgaa acaagcagtg ccggaatctg tgtaaccaaa ccgtctccta tagtcagatt
    40801 agtaaatgtt gaaagggatt cgataaagtc caagtccatt tgcagcattc caattgcaaa
    40861 accgcctaca atatttatta cagtaattat aatacctgca attgcgtcac cctttacaaa
    40921 cttgctggct ccgtccatag ctccgtaaaa gtcagcttcc tgctgtattg cttttcttct
    40981 ttccttggct tccatttcat ttatcaggcc ggaatttaaa tccgcgtcta tggccatttg
    41041 cttgcccggc attgcgtcca gcgtgaatct tgcggatacc tcggaaaccc tttctgcacc
    41101 ctttgtaata actatgtatt gtactataac aagtataaga aacattataa aaccaactat
    41161 aggattactg cctgttacaa agctgccaaa ggcatttata acttcacctg catatccctt
    41221 gcccagtatc agccttgttg tggagaaatt aaggcacaat cggaacagcg tcgtaatcag
    41281 cagtattgtc ggaaaaatcg aaaattgcag cggttccttt atatacatgg ataacaggaa
    41341 aataaccagt gacaatgaaa tattcaatgt aagcagtatg tcaatcaaaa tagtaggaac
    41401 cggtattact ataagaatta ttaaagctac aatcaaaact gctattacat cactatatct
    41461 gcccatacat aggtctcctt ctatttgtct ttcaaactgt atacatatgc cagtacctct
    41521 gccactgcct tgtacagttc actgggtatc tgctgtccta tttctacgct tttatataag
    41581 gcttgagcaa ggggtttgtt ttctactatc ggtacattgt tcttttttgc cgtatccttg
    41641 attttttctg caataaggtc ttttcccttt gctattacca ccggggcttc cgaaacagcc
    41701 tcttcatatt tgacggcaat agcaaaatgc gtagggtttg ttattattac atcggcagac
    41761 ggaacctctg ccatcattct tcttagtgaa agctgcctct gcttttcttt aatttttgat
    41821 tttatttgag gattcccttc aataagcttg tattcgtctt ttatctcctg ttttgacatc
    41881 cttatctctt tttcaaaatt ccatttctga aagccataat caaaaaccga gaggataagc
    41941 agcaccgcag cagccctaat gccaacgctg ataattgtat ttccgatgaa taccattgta
    42001 ctttcaacat tcatattcag caattcagga attaccctgt aattatcaac gagataatca
    42061 taaatgatga atcctataat ccctatttta aaaatagatt taagcaattc cacataagcg
    42121 ttttttgaaa acagcctctt gaagccttca accgggttaa ttttattcaa attcggcatt
    42181 aagggcttgg ctgttaatac agaaccaatc tgaacatagt tcattattat tacgcaaaga
    42241 gcaacaatca gcagtattgg cgcaagtatc tttgcaaata ttaagaggtt ttgcagaaat
    42301 ataagcataa tttcattagt ggtaaacctg gaatcaatat ccttgggaaa actcagggaa
    42361 ttccttacgg caaatatcaa atcaccgaat ataaattttg aaagaaacct catgctaaag
    42421 aatgaaatca acagcactac cgctgtcact aactccctgc tccttggaat ctggcccttc
    42481 tgccgcagct cctgtcttct ctttggagtt gcaggttcgg tcttttctcc gaacagctga
    42541 agatttatct ttaaataagc ataatctttc atcctttagc catactcctt attatcaagt
    42601 agacatagcc gtacatcctg tcaaaggtaa ccgtgataat ctgtataaac acaggaatca
    42661 ttatgtaaag tgtaataagt ccgataccaa ttctcaaggg aaggcctaca atgaatacat
    42721 tcatctgagg aacagttctt gaaagtattc caagggctac ctcggcaagc agagtagcag
    42781 ccattaccgg aatgcttatt ttgaacccta tgagaaatac ctctgtaaaa attgcagcaa
    42841 tattatttgt catagcttcg gtaaaagcaa agccgtcaat aggcaaaacg ctatagcttt
    42901 tgtacagtgc tgccagcaat acatgatgcc cgtcaattgc cagaaaaagt attgtggtca
    42961 ttatatatat aaagtttcct atcataggaa tctgagtatt cgtggtagga tcaagaacat
    43021 taaccattcc gaagccaacc tgcatgtcga ttatctgtcc tgccagataa agagccgaaa
    43081 aaaccataaa gcttacaaat ccgataatta ttcccaccag aaattccttg gctataatcg
    43141 ctgaaaaact gaatatgttg gaataatcaa tccgtacatc ccccagcaaa ggaaccatta
    43201 tgtaggaaca gaaaaatgca agtcctattt tcaaataagc cggcatctct ctccttccga
    43261 ataccggtgt tattacgaaa agagatgtca ttcttataaa aaccagcagc agtaccagga
    43321 atctgtcgac caaaacaaca ctaatactat ccataatcat ttaatataaa cagggatatt
    43381 ctcaaacagc cttacggtaa actcatacat tatgttcatc atccatgggc caaagaccag
    43441 catagtcaag gaaacagcta ttatcttcgg tataaacgat aaagtaggct cttgtatctg
    43501 tgtagttgcc tgcactatgc tcactataag gccgaccaga agccccaatc ccagtattgg
    43561 cgctgaaacc agcaaaacag attttattgc ttcctgtgca acatcaatta ctattccttg
    43621 atccatagat tacacctcaa ttaattaaat cccaaaataa gagatcttac tattagattc
    43681 cagccgtcaa ccattacaaa gagtaatatt ttaaaaggca ttgaaatcat aatcggcggc
    43741 agcatcatca tacccattga cataagtgta cttgatacaa tcatatctat catcaaaaaa
    43801 ggtatatata agaaaaaacc aatctcaaag gctgttttaa gctcgcttat aataaatgcc
    43861 ggtataagcg cagaggttgg tatatcgatc ttggctacgg gctgtttcac ctggcttaga
    43921 ttcaggaaca atgcaagatc cttttctctt gtgtacttaa acataaaatt gcgtataggc
    43981 tctgcagcct tttctattgc agcctcttgt gtaatctcac cgtttgtaaa cggtgtgaat
    44041 gcattttcat tgatttctgt aattaccggt gacattataa aaaaggttag aaacagcgcc
    44101 agccctataa gcacttgatt aggcggcatc tgctgcgtac ccagggcatt tcttgtaaac
    44161 gaaagcacaa ttattatcct ggtaaaagaa gtaagcataa tcagtattga cggtgcaagg
    44221 gaaataatag taagtataaa aagtatctgc atgctcagtg ccacatcctg aggattttcg
    44281 gcagtttcta tatcaagtcc tattttgggt atcggaagcg gttcgcacca aacatcaata
    44341 attcccatta aaatcatcaa taccaaaatc agtataattt ttataatacc gtttttctta
    44401 tacatctgtt ttgttacctc taacaattga tttcagctta ttcagattat tctttattga
    44461 gttcatcccg ttttttttgt tttcatcctg aaagctgctc atataaactt caatactttc
    44521 atcatctcca acagccagtt tctccgggtc cacctccgta agcagtgtta tgctcttctg
    44581 tgtacttccc agcagcaggt attgttctcc tactttaacc agtaaaatgc ttttatcaat
    44641 accaagaggt gctgcctcca ctatcttaag attcttattt ccggcatttt tgaggcttct
    44701 ttttgacaaa aaccttgtag cataataagc ggcagccaga ataacagctg acatgaatag
    44761 gaaatataat attccgtata ttgtactgcc tgtattcata aaacctgctc cttcttatcc
    44821 caataccttc ttaactgcct caataatcct ttctgcctgg aagggcttaa caataaaatc
    44881 ttttgctcct gcttgtattg cttctatgac catagcctgc tgtcccatgg cggaacacat
    44941 aataaccttt gcggcactgt ttattttttt tatttccttt acggcttgta tcccgtcaac
    45001 ttcaggcata gtaatatcca ttataaccaa atcaggatta atttccttgt acttttctat
    45061 agcttttgcg ccattctcag cctctccgct gatttcaaaa ccgttttttg acaatacatc
    45121 cttaatcatc attctcatga atgccgcgtc gtcaacaatc agtataccgt tagccatttg
    45181 attatttcct ccttgtaaat gtaaaatatt gactattgaa ttctgctaag acgcttcgat
    45241 gcacttacaa tatcagtgat tcttacacca aagctttcat caattacgac tacttcgccc
    45301 ttggcaatgt atttaccgtt taccattata tccacaggtt ctcctgcaag cttgtccagc
    45361 tcaagaattg tacccgggct gaactcaaga atatctctta tcaatttttt tgtccgcccc
    45421 agctctactg ttatttcaag cggaacatcc ataataagct caatgctttc tttttcagcg
    45481 ttcaattttt cgacatcaaa ggtttggtat tgtactggct gaacattgac aactttacgt
    45541 tcctctcttc ttgcagcagg ctcatactcc tctctttctt catacccttt gctaagtatt
    45601 gtattgcttt ccggcttttg aggctgagtg ccgggatcgc tgacagccgg ctgcttcact
    45661 tgaggctctt cggctttgcc tacgaatata ggttcagact cggttgaaat gttatccaga
    45721 aggcttctta caagctcttt tgcaaaatct atcggtataa gctgcattat ctcactgtct
    45781 attatatctc ctacttccat cttaaaagca attttaacca ctttatcggc tgaattgaac
    45841 tgctcaagtt cgcttaagtt ctgaaagtat aatataaacg cacggggagg gtaaatatct
    45901 attctcttat tgaacattga agataacgaa gttgaggatg aaccaatcat ttggttcatg
    45961 gcttccccta tggcactaag ctgaagatcg cctaccgaat cggggcttat ctcccccgac
    46021 cctcccatca tcaaatctgt aattattttt acgtcatctt cttttaatat aagcaggtta
    46081 gacccttcca ggccttcttt atactgaacc tgcaccgcaa catacggaat tggatattgt
    46141 attgaaagct cttcccatgt aacaaccgac acttttggcg tggttatgct tactttgttg
    46201 ttcagaagag taaataatgt cgtagctgaa gtccccatgc tgatatttcc tatctcaccc
    46261 agagcatcta tttcttccgt tgtaag
//
LOCUS       DAOOAB010000017        45941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1002_length_45941_cov_15.802489, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000017 DAOOAB010000000
VERSION     DAOOAB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 45941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_45941_cov_15.802489"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..399)
                     /locus_tag="PKU88_05470"
     CDS             complement(214..399)
                     /locus_tag="PKU88_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68770.1"
                     /translation="MNCMHCENCKQNTATYFCLAKNEIIINDSFVANIEKSRKGWKKG
                     DPGYETHRRKLRKEIEV"
     gene            complement(424..1698)
                     /gene="obgE"
                     /locus_tag="PKU88_05475"
     CDS             complement(424..1698)
                     /gene="obgE"
                     /locus_tag="PKU88_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPX68771.1"
                     /translation="MFVDKAKVYVKAGNGGDGRVSFRREKYVPNGGPDGGDGGKGGDI
                     VFIADENMRTLMDFRYKRKHIAENGEMGGTNNSSGKSADDLYIKVPAGTILRDAESGL
                     LLGDLKNPGDKAIVALGGRGGKGNQHFATPTRQAPRFAQPGEPGMERWIELELKLLAD
                     VGLIGFPNVGKSTFLSVVTSAKPKIANYHFTTLVPNLGVVDLGEARSFVIADIPGLIE
                     GAHSGVGLGHDFLRHVERTKVLIHLLDASGLEGRDPLEDFDKINEELRLYSEKLANKP
                     QVVACNKMDIPAARENYARVAETLSRRGYEVFAISAATRDGIAPLMDRVFNLLESTKD
                     EEPVEEIVDLMYHINTEDKPFTARKENGVFIVEGKLIEQLLASVNLDDNDSIKYFQKV
                     LRKRGIVDELRQLGVKDGDTIRMLDLEFEYFE"
     gene            complement(1700..2380)
                     /locus_tag="PKU88_05480"
     CDS             complement(1700..2380)
                     /locus_tag="PKU88_05480"
                     /inference="COORDINATES: protein motif:HMM:NF026040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HPX68772.1"
                     /translation="MSIKNSGIYDVPDIESEKERYIESLKEHLSEQRHDFFNLLQVLY
                     GYTQLKKPDKVLEHISEYCKRMENIGRLYNCKCIKLADLLYNKVKEAESADLKLEVSV
                     EVSFDSAVRILDEVPVVYVVDHVIMGFLYVLDEKGMKNAHIIYNLKEISEGFLMEIYC
                     REIRKGLFGPVDFIFPEKVIYWKKAARGVLGFDKIMEYCSDVKLDGKVLGDGTTFILS
                     ISKVNKGE"
     gene            complement(2456..2737)
                     /gene="rpmA"
                     /locus_tag="PKU88_05485"
     CDS             complement(2456..2737)
                     /gene="rpmA"
                     /locus_tag="PKU88_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPX68773.1"
                     /translation="MFKMNLQLFAHKKGVGSSRNGRDSHAQRLGTKKADGQSVLAGNI
                     IVRQRGTKIHPGINVGIGKDDTLFALIEGVVKFERKGRDKKQASVYPVQ"
     gene            complement(2740..3072)
                     /locus_tag="PKU88_05490"
     CDS             complement(2740..3072)
                     /locus_tag="PKU88_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPX68774.1"
                     /translation="MIKIVIYKAREGKITGFKISGHSGYGTHGTDIVCSAVSALGQTA
                     LLGLLKVAEAEVHYRIEEGYLDCSIAGAGSDRKRIMCDAILETMYEGFKSIKDSYKRH
                     IDIVEEEV"
     gene            complement(3102..3413)
                     /gene="rplU"
                     /locus_tag="PKU88_05495"
     CDS             complement(3102..3413)
                     /gene="rplU"
                     /locus_tag="PKU88_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPX68775.1"
                     /translation="MYAIIKTGGKQYMVKAGDVLAVEKLDAESGEKVVFEEVLAVGDN
                     GELQVGNPVVAGAKVEGTVVGNGKAKKVIVFKYKPKKDYRRKQGHRQPFTKVKIEKIN
                     A"
     gene            complement(3544..5067)
                     /locus_tag="PKU88_05500"
     CDS             complement(3544..5067)
                     /locus_tag="PKU88_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPX68776.1"
                     /translation="MKEIIIDVGSRQTRVALLENKELTEIYIERQSEERITGNIYKGR
                     VENVLPGMQAAFINIGLDKNAFLYVKDALPNTYFDEDEEYVSSAQYKDYQIDELLKAG
                     QEIMVQIMKEPIDSKGARVTTHITLPGRYAVLMPTVEYIGISRRIEDEEIREKLKKLA
                     EELKPKGMGLIIRTAAEDCTEADLSNDINFLLKLWDNIKQKQKTGAAPRIVHKDMNII
                     YRTVRDMFTKDIDRLVINNKEHYEKVRELVTIISSHFKNKVEYYNKSYDIFEYYQAEP
                     QISRIINRKVWLKCGGYIVIDQTEALTSIDVNTGKYVGTVDLKDTVLKTNIEAAKEIA
                     KQLRLRDIGGIIIIDFIDMADEIHESTVLEALKNALKKDRTRTNVLGITQLGLVEMTR
                     KKVRQRIEKILMAPCPYCKGEGRILSSETIVKKIEKEVNSLFFNTGAMAVFLEIHPDV
                     EELINYYIDEDMFELGKSFNKPVFVRTSAELHREGYIVKGIGMSEITELRKKGCREI"
     gene            complement(5103..5816)
                     /locus_tag="PKU88_05505"
     CDS             complement(5103..5816)
                     /locus_tag="PKU88_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPX68777.1"
                     /translation="MMHIRLKFTKGNEVKYISHLDLMRVFQRALRRTGIPISYSSGFN
                     PHMEISFGAPLSLGVTSNAEYVDLKLAEPMDVREIQRRLNDSLPDGIRILDGIEIREN
                     HKSAMSVVSHSRYRIRIFMENVSSDELTKKIEEFAAQKEIKIMKEQPKKGFELKEIDI
                     KPLLVSMKLIESKEDTYTIDCLLLSGSRGNLKPGLLIKAFKEFTGYDIQSIRINREQV
                     YGLKNGKLLDLLEYESAEA"
     gene            complement(5813..7669)
                     /locus_tag="PKU88_05510"
     CDS             complement(5813..7669)
                     /locus_tag="PKU88_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPX68778.1"
                     /translation="MKYDNTREFIYEELLSKVEKPGRYIGTEWNSIHKNPETVDIRFA
                     FCFPDVYEVGMSHLGMKILYHLLNDQQDIFCERVFAPWPDMEEVMRKTGIKLFGLESR
                     DFIDSFDFVGFTLQYEMSYTNIVNMLDLAGIPKRSGERNEGHPFVVAGGPCAYNPEPL
                     YDIVDFFMLGEGEEQLLEVMEHYRKWKDSKGTREEFLKMAASVRGVYVPKFYNVEYNE
                     DSTIKSIKPIKEDIPGKVRKRVIKNLKGSYFPDKIVVPFIDIVHDRIMLEIFRGCTRG
                     CRFCQAGMIYRPVREREVEELLEIADKLIQSSGYEEISLSSLSTSDFSKLNELVERLV
                     SRYKSSRIGLSLPSLRIDSFSLKLVQEVQKVRKSGLTFAPEAGTQRMRNVINKGVDEE
                     DLLNSVGAAFETGYSSVKLYYMIGLPGETMEDIQGIADLTFKVVDRYYQVPKEKRGKG
                     LNVTASASSFVPKPFTPFQWEPQDPINTLIEKQRYLQKAIKKKQITFNWHEPYVSYME
                     AVFARGDRRLCDVLIRAWELGCKFDGWDQYFDFEAWLQAFKDTGADPDSYAARRRELD
                     EVLPWDHIDVGVTKKYLRGEYKKSLKGELTRDCRTACTGCGINLLQEGGVCK"
     gene            complement(7999..8838)
                     /locus_tag="PKU88_05515"
     CDS             complement(7999..8838)
                     /locus_tag="PKU88_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPX68779.1"
                     /translation="MRIKLNVFFILFLFVSCIIGWLQQSLIMFASVLLHEFGHVLTAK
                     KSGIKVYEVELMPFGGISRMEELSKLGGAAEAVVSAAGPVTSFVLSLTFSFFREYSGV
                     LESACRYNLIICLFNLLPVIPLDGGRIARNLLCFLMGYRQATKILSSAGKIAAFLLLG
                     FNIHMLAAGSKSGALIIAAVFIYIGALKEEKNSSYYYLFTGNNTKNAMIARGRIRKRF
                     IKVQEDNLIRQIVNKFSPVTQCCVEVADAKGRHKRILSEEEIIKGLLQYGYDGKIAQI
                     ING"
     gene            complement(8872..9606)
                     /locus_tag="PKU88_05520"
     CDS             complement(8872..9606)
                     /locus_tag="PKU88_05520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX68780.1"
                     /translation="MDNNNFENIPRPRYVKEYRKLGAAFKTRRYGERNFGERFLMQSF
                     ISLAAIAAVLLVNNINIGIANTISDGIRTTINWNMDFSKVLGTFRNVGDIIPEAKENL
                     GVAQVGIESFEDSPSFIFPVEGEITSEYGERIHPVFKTVRMHTGIDIDAAIGTPIKSS
                     AAGTVTTVGEDETNGKYVSIRSGKYDVIYAHCYKITVKEGQKVQQGEIIGETGDTGIT
                     SGPHLHFEIQEAGKPVSPMEMLMEVQ"
     gene            complement(9776..10057)
                     /gene="minE"
                     /locus_tag="PKU88_05525"
     CDS             complement(9776..10057)
                     /gene="minE"
                     /locus_tag="PKU88_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HPX68781.1"
                     /translation="MDLFKWFNRSTSSKDIAKERLKLVLIHDRANVSPQFLEMIKGEL
                     IKVISDYMEIDETGLEIKLTRTKREIDDSTVPALVANIPIKRMKGNVNI"
     gene            complement(10073..10879)
                     /gene="minD"
                     /locus_tag="PKU88_05530"
     CDS             complement(10073..10879)
                     /gene="minD"
                     /locus_tag="PKU88_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPX68782.1"
                     /translation="MGEVIVVTSGKGGVGKTTTTANIGTGLALKGKSVVLVDADIGLR
                     NLDVVMGLENRIVYDLVDVVEGVCRQRQALIKDKRFPNLYLLPAAQTKDKNAVNPEQM
                     QKLTGDLAEEFDYVILDCPAGIEQGFKNAIAGANKAIVVTTPEVSAVRDADRIIGLLE
                     ASGLRNPMLIINRIKMDMVKRGDMMNIDDMIDILAIDLLGVVPDDDLIVVSTNRGEPV
                     IAIEQSLAGQAYRNIVRRVLGEDIPFMNLESEDGFFKRVAKLFGLNKKKQ"
     gene            complement(10928..11593)
                     /gene="minC"
                     /locus_tag="PKU88_05535"
     CDS             complement(10928..11593)
                     /gene="minC"
                     /locus_tag="PKU88_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HPX68783.1"
                     /translation="MAENAVIFKGTKDGLYIILKEEMDLSTIKYNLEKKIKPSKRFFE
                     GANIMNFKGKKLTQDEFDELKEIMEQEYGMTVIGSYNDKDYPVHEEHRGAKKPEILEQ
                     LPFDNVHKGEALIVRATLRSGQLIQYKGDVVVIGDVNPGAHIKSAGSVIVMGTVRGII
                     QAGANGDYGAFIVAYKMQPSQLRIGDIITRSPDGMGSKSDNPEIALVKQGMIVVEPYL
                     KNR"
     gene            complement(11694..14135)
                     /gene="mrdA"
                     /locus_tag="PKU88_05540"
     CDS             complement(11694..14135)
                     /gene="mrdA"
                     /locus_tag="PKU88_05540"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPX68784.1"
                     /translation="MFKKYFSNRLDIIRAVTVLVFLVMAFRLADLQLVKGEYYRKKSE
                     TLRTRNISVTAPRGHIVDKFGRLIAGNKQSYSVNIMKTDVPQDTLNDIALSVINIIEQ
                     NGDTYKDEIPILINPIRFSYQDDEINWKKKYGIPESASAKDAFMKLRFDYEIPADTID
                     LEAYEIMRDEKSVELPFDINEFEFSFRKSEIKWKQSHGFGPEKSASEIFDVLCSKYKI
                     PRDIYDDEKAKKILAVKYLVGQNRYKAYEPVEIAANIKMETRAQIEESKIFLPGVEII
                     QKPIRSYAGRDFASHIIGYMGMIGSELEELASKGYTPQDLIGKSGIENSMEEYLKGKD
                     GSRQIEVDVNGTLINTINEIDPVPGDTVFLTIDAKLQKTAEESLARTMENIRNGIVKG
                     KPYPNATSGAAVAIDVNTGKILALASEPRFDPNLFAAGISSEDWKNLQPKSKDVYAPK
                     PLMNNAISSIFSPGSTMKLVSAVAGLESKVITPSETIYDKGYYTAIPGVSPSCSIFKS
                     TGIPHYSQNVSQAIKNSCNYFFFEVGRRMGGEVFEEYAKKFGFGEKTGIELLSEYKGT
                     VEGPTNKKELFRRYLDNYLVNNLQINDKNVRDEIKGYLDNNPGESKVRKRLKELGIND
                     SKAIERVWRYIRDSKYQPGDVLNATIGQGMNGVTPLQLANAVASIVNGGTRYRPYLVD
                     KVIDYDGNVKLEKKPEVAEKNDISPANLEAIKRGMYAVTNEAGGTARSAFTGSKVIIA
                     GKTGTAEVGKGYDAHAWFAAFAPYEKPEIAVVVMITQGGHGNYPAPVAREIIEAYLAP
                     EATKDNMHIDNEILP"
     gene            complement(14181..14693)
                     /gene="mreD"
                     /locus_tag="PKU88_05545"
     CDS             complement(14181..14693)
                     /gene="mreD"
                     /locus_tag="PKU88_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPX68785.1"
                     /translation="MRIAIICILAVFNVILESTLFQCTRINGIKPDFSIMIIVAYAIM
                     RGSSFGTFIGLGIGLLIDVMYARVIGINALSYMVTGYIIGQAHEKVFKDSFIPPFIFN
                     LIAIIILQHSFLLASYFSNDFPSAGSSYIYMLLQVILPQALYNAIAGSIVYRFIYKLD
                     ETSFMDRRIY"
     gene            complement(14711..15520)
                     /gene="mreC"
                     /locus_tag="PKU88_05550"
     CDS             complement(14711..15520)
                     /gene="mreC"
                     /locus_tag="PKU88_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPX68786.1"
                     /translation="MKIRNKLLISVVLISILLISLIAVTSQGRTRPGVIDGIFGRIIT
                     PVQRVFYSSGEFFKNTFKSLYDLKDLKTENENLKAEVEKLKAQNRQLIQMALENGRLR
                     SLLEFKDTNTQFEYIGANVTGRDPGNWYDVYTIDKGSGDGIQVNDAVIVGHGYLVGKI
                     IETGANYSKLMAIIDERSSISIIVNRTRDMGIVSGSADSDVIAIMELEADIVKGDDII
                     TSEYSTLPKGLHIGKVKSVEKQEKKLQKVVIIEPSVDFKRLEEVFVIKASK"
     gene            complement(15543..16571)
                     /locus_tag="PKU88_05555"
     CDS             complement(15543..16571)
                     /locus_tag="PKU88_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPX68787.1"
                     /translation="MGLFNFLSKDIGIDLGTANTLVYVRGKGIVVREPSVVAMKLDTN
                     AVMAVGDEAKKMIGRTPGNIIAIRPMRDGVIADFNVTHRMIKYFIVRAYAGSPVGKPR
                     VLVSVPSGITEVERRAVEEAAMQAGARIARLIEEPMAAAIGAGLPVGEPTGSMIVNMG
                     GGTTEIAVISLGGIVTSKSIRAAGDKQDEAIVHYIKRMYNLAIGERTGEEIKIVIGSA
                     YPNTAEGSMDIKGRDLVTGLPKTIKVTSEEILEALREPIAIILDGIKSTLEKTPPELA
                     ADIMDRGIMLSGGGALLHGLDILVKHETGMPVQVAENPLDCVAIGTGKALEQYDEISK
                     SYSGKHRR"
     gene            complement(16713..17402)
                     /gene="radC"
                     /locus_tag="PKU88_05560"
     CDS             complement(16713..17402)
                     /gene="radC"
                     /locus_tag="PKU88_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPX68788.1"
                     /translation="MQRGHATIKNLPEEERPRERLARHGASVLSNAELLAILLRTGTR
                     EESAISLAHRILVQEQGLRYLADSNVEQLSAINGIGKAKAAQIKAAIELGKRLAAFEP
                     GADKPLKCPQDVAGLLMEEMRYLKKEHMKLVLLNVKCNLISVEEISVGSLNASIVHPR
                     EVFNPAIRKSSASIIMVHNHPSGDPAPSSEDISITARIAEAGKLIGIELVDHIIIGDG
                     KYVSMKEKGLF"
     gene            complement(17458..18027)
                     /locus_tag="PKU88_05565"
     CDS             complement(17458..18027)
                     /locus_tag="PKU88_05565"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPX68789.1"
                     /translation="MRIILASNSPRRKELLSQTGIKFEVIPSSFEESIAELPASKLVE
                     HFAYMKAKDVAKSVKGDALVIGSDTIVYCDEIMGKPRNREEAFCMLTKLSGKQHLVIS
                     GISIINTATGESLTEHESTRVQIKELSSAEITAYINTGEPEDKAGAYAIQGLGSLFVE
                     GIQGDYFNVVGLPIFRLRKMLEHFGVKLI"
     gene            complement(18056..18310)
                     /locus_tag="PKU88_05570"
     CDS             complement(18056..18310)
                     /locus_tag="PKU88_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HPX68790.1"
                     /translation="MLRREGGRNGWIMLLMVVCGLVVGGFLGELLGKYFPILKYGYNL
                     GVSAHEWNFHILRLTFGFDFNINMFSILGIVIAIYIYRKL"
     gene            complement(18730..19707)
                     /locus_tag="PKU88_05575"
     CDS             complement(18730..19707)
                     /locus_tag="PKU88_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HPX68791.1"
                     /translation="MFNSIGLPVIVLGGMAVFFGIVLAYASKKFAVEVNPKVTEIRDV
                     LPGANCGGCGFAGCDALAEAIASGNAPISQCPVGGSELAAKLAVIMGVEAGSEERQVA
                     RVICCGDSNNAAEKYEYQGIKDCKAAEMLAGGSKSCRYGCTGLGTCVRECPFDAIHIV
                     NGIAVVDEEKCTACKKCIAVCPKGIIELVPVSKKVRVLCRNQDKGKATMQACKIGCIA
                     CQKCVKACKFDAIKVENNVARIDYSKCVNCMMCAEVCPTKTIYANFADRKKAFIIEDK
                     CIGCTICSKNCNFEAIEGELKGKHKVLDDKCVGCGQCFAKCPKDAIEMK"
     gene            complement(19741..20325)
                     /gene="rsxA"
                     /locus_tag="PKU88_05580"
     CDS             complement(19741..20325)
                     /gene="rsxA"
                     /locus_tag="PKU88_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HPX68792.1"
                     /translation="MKLGLIIISAIFINNFVLSQFLGICPFLGVSKKIDTAVGMSIAV
                     TFVMGLASVICWAVQIFVLDPMGIGYMQTIAFILVIAGLVQFVEMVVKKTSPTLYQAL
                     GIYLPLITTNCAVLGVAILNIDKSYNLIESLINGVGAGLGFSLAIVLFAGVRERLELA
                     PIPKALEGFPIAMIAASLFSIAFLGFSGLFSGLL"
     gene            complement(20340..20936)
                     /locus_tag="PKU88_05585"
     CDS             complement(20340..20936)
                     /locus_tag="PKU88_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPX68793.1"
                     /translation="MNLWKDFSNGLIKENPTFRLVLGTCPTLAVTTAVFNGIGMGIAA
                     AAVLIGSNLIISLIKKIVPNEVRIPIYIVVIATFTTIVQMLIQAFSPALDKSLGIYIP
                     LIVVNCIIIARAEAFAAKNPVANSIMDGLGMGIGFTVALTIISSVREILGAGTFLGMP
                     LFGEAFEPFLIMILPPGGFLVFGLSIAAFNKITSLRKS"
     gene            complement(20959..21543)
                     /locus_tag="PKU88_05590"
     CDS             complement(20959..21543)
                     /locus_tag="PKU88_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPX68794.1"
                     /translation="MRDYIRLAGILLLVCVVMAASLGYTNAITYDRIQEQIIKANDEA
                     RKEVLPDADAFEILDDNAFSEIKSNGKYNFVTEIYTAKAGGNVVGYAVKAVPKGFGGT
                     IDVVVGIGTDGIIQGIKVGNNTETPGLGKNSATPKFQNQFKGKAWDNTINVIKSGTPK
                     DNEIAAVSGATITSRAVTDGVNQALEAAKKLSGK"
     gene            complement(21536..22531)
                     /locus_tag="PKU88_05595"
     CDS             complement(21536..22531)
                     /locus_tag="PKU88_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPX68795.1"
                     /translation="MENSRFLVSSSPHIRSGETTQRIMLDVIIALMPALLAGVFYFGL
                     NALTLTAVAVVFAVTTEAVMQKILGKPVTVNDLSAVVTGILIAFNVPVTLPLWMAAVG
                     SIFAIAIAKQCFGGLGYNFINPALAARAMLLASWPVRMTAWVAPGTDAVSTATPLALV
                     KMGADALSGATHWEEGVEAVGQTLPGLWDLFIGNIGGCIGETSALALILGGAYLVYRR
                     VISIRIPVSYIAAVAVFTLLFGRFDFAAAIYHVLAGGLFLGAIFMATDYSTSPITPKG
                     QIIFGIGCGFLTSVIRFYGGYPEGVSYSILLMNLATPLIDKYTMPRKFGEVKKSA"
     gene            complement(22546..23877)
                     /gene="rsxC"
                     /locus_tag="PKU88_05600"
     CDS             complement(22546..23877)
                     /gene="rsxC"
                     /locus_tag="PKU88_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPX68796.1"
                     /translation="MRTLTFKGGTHPPHSKKATESLPIERANEPKVVVIPMQQHIGAP
                     CEPIVQAGDEVKVGQKIGEAKGFVSVPVHSSVSGKVAVVEPRLYSGGTAVPCIVIEND
                     MQNTVSDQVVPKGDISVLSAEDIKNIIKEAGIVGMGGATFPTHVKLAPPPDKNVDTVI
                     LNGAECEPYLTSDHRLMLEHPENVVFGLQALMKALGVKKGFIGIETNKPDAIEKVFEA
                     ARGIEDIEVVALKTKYPQGAEKQLIYACTGREVPSGGLPADAGVVVNNVGTAAAVATA
                     IKTGMPLIERIVTVTGEGVNNPKNLLVKIGTSFRDIIEQCGGLKGKVGKIIAGGPMMG
                     ITQFSLDIPAIKGTSGILVLSEEEARLPEPSNCIKCGKCVETCPINLMPVNISMYSLA
                     NRYDEAEALNAMDCIECGACSFVCPAKRPLVDSIRVAKREILARRKKAQSK"
     gene            complement(24257..26050)
                     /gene="nuoF"
                     /locus_tag="PKU88_05605"
     CDS             complement(24257..26050)
                     /gene="nuoF"
                     /locus_tag="PKU88_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPX68797.1"
                     /translation="MEMYRSHVMICRGTGCTSSNSEQIAKNFEEEIKKHGLEKEVVVE
                     RTGCFGLCALGPVVIIYPEASFYSMVKPEDVEEIVKEHLVKGRIVKRLLYAESVEENR
                     LKALEEVDFFKKQVRVALRNCGIINPENIDEYIAFDGYKALEKVLTKMTPAEVIDTVK
                     RSGLRGRGGAGFPTGMKWEFASKSVNDQKYVLCNADEGDPGAFMDRSVLEGDPHSILE
                     AMTIAGYAIGASQGYIYCRAEYPIAVKRLGIAIGQARLYGLLGDNIMGTGFKFDIELR
                     LGAGAFVCGEETALIASVEGQRGMPRPRPPFPANKGLFAKPTILNNVETYANIPVIFN
                     KGPEWFASMGTEKSKGTKVFALGGKISNTGLVEIPMGTSLREVIYEIGGGCPNGKKFK
                     AVQTGGPSGGCIPEEHLDSPIDYESLAALGSIIGSGGMIVMDEDNCMVDIARFFMDFI
                     VDESCGKCTPCREGTKRMLEILERITEGKGEEGDLDKLESLAKNIKNAALCGLGQTAP
                     NPVLSTIRYFRDEYEAHIKDKKCPAGVCKELLDYFIIADKCKGCGICAKNCPQQCISG
                     QPKTPYTIDSSKCIKCGVCMQKCPFKAIIRK"
     gene            complement(26104..26472)
                     /locus_tag="PKU88_05610"
     CDS             complement(26104..26472)
                     /locus_tag="PKU88_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPX68798.1"
                     /translation="MKTIQELEEIRKKTLDTINIRKDRSGTRIVVGMATCGIAAGARP
                     VLLTIMDEIKKHDLSDVVVAQTGCIGMCRLEPIVEVYKPGEEKVTYVKVNPSKAIRIV
                     NEHIMKGKVVEGYTIGAESK"
     gene            complement(26474..27037)
                     /locus_tag="PKU88_05615"
     CDS             complement(26474..27037)
                     /locus_tag="PKU88_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX68799.1"
                     /translation="MKDLSLHILDLSQNSISAGAGLVEISIEEDTKEDRLTICINDNG
                     KGMDKDFLEKVMDPFVTTRTSRRVGLGIPLMLAACSRCEGDLVVESEKNVGTRLKAVF
                     KHSHIDRAPMGDMAETMLSLILAGSDVNRNVDFVYRHIIDGRVFCLDTREIRTALGND
                     INLGEPEVLMWIKDYINEGLKNIRGGV"
     gene            complement(27045..27515)
                     /gene="nuoE"
                     /locus_tag="PKU88_05620"
     CDS             complement(27045..27515)
                     /gene="nuoE"
                     /locus_tag="PKU88_05620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPX68800.1"
                     /translation="MSDAAMKENMEKLEDFIQKHKNQKGALIPILHEAQELFGYIPEE
                     VQKRIAEVLHIPAAEIFGVATFYSRFTLTPRGKHTISVCLGTACYVKNSKGVLERLEE
                     ELNVKTGGTTADGKFTLEATRCLGCCGLAPVMMIGEDVYGKLVPDDIPDIVKKY"
     gene            complement(27797..28510)
                     /locus_tag="PKU88_05625"
     CDS             complement(27797..28510)
                     /locus_tag="PKU88_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPX68801.1"
                     /translation="MEFAVDFHIHSALSPCGDEDMTPCNIVNMALLKGLDIIAVTDHN
                     SCGNLPAVMEAAKENGLMVIPGMEVQTKEEVHLLCLFKRMEGAMKFAEIVYNSLPDIE
                     NNEEIFGRQLIFNSSDEIIGKEHKLLLSSTILSVNDVFILTRGLGGVCIPAHVDRQGF
                     SIITNLGFIPPDLKVKTVEISKRCTPDAAFKKYPFLSRFNYIISSDAHYLEDISERDF
                     FIELDCLTVSELFNKLDPK"
     gene            complement(28512..28847)
                     /locus_tag="PKU88_05630"
     CDS             complement(28512..28847)
                     /locus_tag="PKU88_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX68802.1"
                     /translation="MLVRDAGEKIGLKLLAGEAGTSREITGVYICDLLSWVMAHAGKG
                     DAWITVQTHANIVAVAALLELGCIIIPEGVEVEEDTLRKAEDEGVPVYQSGLSAYGIA
                     KELYRMGIE"
     gene            complement(28859..30139)
                     /locus_tag="PKU88_05635"
     CDS             complement(28859..30139)
                     /locus_tag="PKU88_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPX68803.1"
                     /translation="MKEKYFHSVVLDADRCVGCTSCLKVCPTEAIRLKEGKACIIGEK
                     CIDCGECIRICPNHAKNAVTDSKDILKKYKYTIAIPSLVLYGQFRGNVGINEMLTAVR
                     KIGFDEIFEASAGTEIITNVIKNHILKNPEKKRPIINSSCPAALRLIQIRFPELLPNI
                     ADIETPVEIAARLAKAEAVRRTGFSMEEIGVIYISPCTARMTSVKHPIGIKKSYIDGV
                     LSMKDVYGMMLRNLGSNTEDLTRSTKESLLWPIIGGQGKPLGIKNYLAVDGIHEVIKI
                     LEEIEMDKLEGLEFFEGMACVGGCVGGPLTIENPFIARRRMKTLAKGLPDSNPSDEEI
                     KGQIEMYKSGFIRLTEAIEPKAVMRLDDDICKSIKKMEKIKEMLKDLPGIDCGVCGAP
                     TCKAFAEDIVKGENRNAVCIVKTMKSFNKEKPQS"
     gene            complement(30141..30581)
                     /locus_tag="PKU88_05640"
     CDS             complement(30141..30581)
                     /locus_tag="PKU88_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX68804.1"
                     /translation="MEDIKYVFDVKKDDFVRAGETSSKVKKILRELGIDSSIIRKISI
                     AAYEAEMNIVIHSTGGQIVLEITPGIIRITANDRGPGIADVELAMKEGYSTATDKVRE
                     MGFGAGMGLPNMKRCSDRLYVTSSEGTGTLIVIEVDLKAGEQGE"
     gene            complement(30629..30979)
                     /locus_tag="PKU88_05645"
     CDS             complement(30629..30979)
                     /locus_tag="PKU88_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPX68805.1"
                     /translation="MKICEVKEVLNAELLCGDELLENEVSYAFGSDLMSDVLAFVKGK
                     TLLLTGLTNQQVVRTAEMADLSAIVFVRGKKPGEDIVQLATEKDIALLLTRDTMYTAS
                     GKLYSNGLEGVSID"
     gene            31144..31686
                     /locus_tag="PKU88_05650"
     CDS             31144..31686
                     /locus_tag="PKU88_05650"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPX68806.1"
                     /translation="MIGLSSILSGILRQKLLEIQNQLPPYVKIIKDNPPSFEDVLNKE
                     INEQSDVQNNYGGDYSHLIKAAAKKYNINENIITAVMKAESGFDPTAVSRTGAMGLMQ
                     LMPETAEALGVHNAFDPRENIEGGVRYLKDMLTEFGGNLELALAAYNAGPNAVKKYGG
                     IPPYEETQNYVRKVMSSLKK"
     gene            complement(31736..32269)
                     /locus_tag="PKU88_05655"
     CDS             complement(31736..32269)
                     /locus_tag="PKU88_05655"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX68807.1"
                     /translation="MLTLNNKLDNLIFRDITKEDLKAVLNLYNQNDQNIYATGIDRRM
                     SLRDIKEKYLEVLVNSHEFFTGIFLEKEDALQLVGVIKGRIDYENSEEAWISSILVDN
                     RYQRLGIGTKAAEALINMLNKSYDVKRFYIGIIARNSVGKCFWQKLEFSYFRTIEQYI
                     KLNALAEDFIIMKREVL"
     gene            32548..33582
                     /locus_tag="PKU88_05660"
     CDS             32548..33582
                     /locus_tag="PKU88_05660"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPX68808.1"
                     /translation="MAEIRIMRTSDCKPGMVLASDIINDYGAVILYQNSILDEYSISK
                     LMKLGLNFVKIYKDYEFKEKKHAVIEAQYNNNLEEFKEIIWDISCGKTLEMERVSEVS
                     RNISSNFDSIHDLVSCLSKVRSISEYTYAHSLNVSLLCSLLGSWLNLGHKHIEQLSYC
                     GILHDIGKSKIPPEILNKPDTLTEEEFDKIKQHPLIGYKMLEDNKEISKDVALGVLMH
                     HEREDGSGYPFGFKSERIYYYAKIVAVVDTFDAMTSNRIYKKRQTPFDVLEMYESEYL
                     TKCDAGIMLTFLKHISSYYIGAKVRLSDSSIGEIVYINTNRISRPLIKSKETIIDLSM
                     VPELKIVEML"
     gene            33671..34600
                     /gene="fba"
                     /locus_tag="PKU88_05665"
     CDS             33671..34600
                     /gene="fba"
                     /locus_tag="PKU88_05665"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPX68809.1"
                     /translation="MPLVTSKEMFRKAYESQYAVGAFNVNNMEIIQGIVDAAKEENAP
                     LILQVSAGARKYAKHVYLMKLVEAAVEDTGLPICLHLDHGEDFEICKSCIDGGFTSVM
                     IDGSKHPFEENIAVTKRVVEYAHERGIAVEAELGKLAGVEDTVNVSAKDATYTDPDQA
                     VEFVERTGVDSLAIAIGTSHGAYKFKGEPKLDFERLEKITKLLPGFPLVLHGASTVLP
                     EFVELCNKYGGNVAGAQGVPEDMLLRAGKLGVCKINIDTDLRLAMTASIRKHFAENPG
                     DFDPRQYLKPAREAIKNMVKHKMRNVLNCSGRM"
     gene            complement(34738..35181)
                     /locus_tag="PKU88_05670"
     CDS             complement(34738..35181)
                     /locus_tag="PKU88_05670"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPX68810.1"
                     /translation="MDKREEALEKVMVYFNGGYNCCESVVMAVCDYLGADKEIPQKIA
                     TPFGAGMSKNGSNCGALNAAFICMGLVGGRRSSEDSKDTSFLPADRIFGKFKEKYGST
                     FCYDITGLDMKKTAETEEEKAKRHNELCGPLVLQVTQWILEELNP"
     gene            complement(35317..35733)
                     /locus_tag="PKU88_05675"
     CDS             complement(35317..35733)
                     /locus_tag="PKU88_05675"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HPX68811.1"
                     /translation="MKGYKWWDMNSSIRIIRKSEGKVSTWSGGTTTQLYIYPGNAVYA
                     DRNFRWRLSSAKVAADESTFTPLPGISRILMIIEGEVEIEHQGHHRTVLKAFEKDSFS
                     GDWTTICKGKAADYNLMTDKSCSDKKINIIRTTIYY"
     gene            complement(35779..36294)
                     /locus_tag="PKU88_05680"
     CDS             complement(35779..36294)
                     /locus_tag="PKU88_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68812.1"
                     /translation="MSRKMAFKGIFAGIAVVFLYLSTIMPTGKLTLYFLASLPVAITV
                     AELGAGAGIAVYFAASLLNILITGNITGAVPFVFFFGHYPVFKYFIEKGRKAAVELLL
                     KLTVFNMSIFLWYLFFKNLFITALPAKFTGGSILTAVFITAMQAVFLVYDYVFSRLLF
                     YYKSKLSLFRR"
     gene            complement(36399..37028)
                     /locus_tag="PKU88_05685"
     CDS             complement(36399..37028)
                     /locus_tag="PKU88_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPX68813.1"
                     /translation="MRNPYEVLGIREGTSVEGIKRAYKELVRKYHPDQYQNNPLSDLA
                     EEKLKEINEAYDYLMKKAESEERNSSYRQQEEPRYGNNRENGDRGLYDQARIYVNMGN
                     TAAAEEILNRISFRGGEWYYLRGLIFMRRGWYNEAVTCLKTAVDMEPSNYEFRDALNS
                     IMNANRQYQSTVYERRGYTSGPDLCTLCQCMICTDCCCECMGGDFIRCC"
     gene            complement(36997..37911)
                     /locus_tag="PKU88_05690"
     CDS             complement(36997..37911)
                     /locus_tag="PKU88_05690"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HPX68814.1"
                     /translation="MFGYIKPSVQELKIKEYELFRGYYCGLCKSMGRSFGVLSRFALN
                     YDSVFLGLLLASVHGEKPVLKKESCIANPVKRKWIVKESPGIDFAADINVLLTYYKLK
                     DDIRDEGGWLPTFLKLVFHKGYKRAALTNKDIDRIIASSVSSQISLEKKGCSSTDEAA
                     EPFANMMMQLLASGYSGDDKSVPRILGWIGYNLGKWIYIIDAFDDMERDIKSGSYNPL
                     LRQYKYENQDIKDFKASIAEEVKVGLLQALSQATGSLELLKLDNKGIISNIAYEGLYG
                     KTERVLNCIAGEKRSCVRDEESIRGIGY"
     gene            complement(38191..38601)
                     /locus_tag="PKU88_05695"
     CDS             complement(38191..38601)
                     /locus_tag="PKU88_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HPX68815.1"
                     /translation="MVKFILGVKGSGKTKRMIELANESGRTAKGNVVYIDRDRNRIHD
                     LNRRIRLIETGDFQLENLKSFYGFICGIISQDFDIENIYIDGQKIVSNAQDDCLEGFI
                     KNLEILHEKFGVDFTISCSRAIEGIPEFLKSYLI"
     gene            39151..40605
                     /gene="nhaC"
                     /locus_tag="PKU88_05700"
     CDS             39151..40605
                     /gene="nhaC"
                     /locus_tag="PKU88_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPX68816.1"
                     /translation="MSTKTKREATLFEALIPVVITIGFLMYAVLPQFGVGQDVHVPLI
                     LGALIASIVAITRLGYTWKEVEDGIVSTISDTMQAILILAIIGMIIGTWILGGIVPTL
                     IYYGLQILSPSFFLIAACLLCCVVSLATGSSWTTAGTVGIALMGVAAGLGVNPGAAAG
                     AIISGAYFGDKMSPLSDTTNLAPAMAGANLFDHVRHMVYTTGVSLIIALIGFGILGAG
                     YAGKELDISAINEILALMSDNFVINPLLLLVPLIVIVMVARKVPAIPGLFIGTLLGGL
                     AAMIFQGASFAGVMDAMQYGVVSETGNAIIDELLTRGGYQSMMWTISLILVALTFGGV
                     LEKTGMLETIGKVILGYAKSTGALVTATIITSIFTNVLAGDQYLALVLPGKMYKDEYR
                     KRGLAPRNLSRCLEDAGTLTSPLVPWNTCGATMSTYLGVPTIQYIPYCFLNLVNPIVS
                     IVFGFTGITMMKLEDDPSAPEYKGPKSKNENVSA"
     gene            complement(40645..40791)
                     /locus_tag="PKU88_05705"
                     /pseudo
     CDS             complement(40645..40791)
                     /locus_tag="PKU88_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
     gene            41012..42139
                     /locus_tag="PKU88_05710"
     CDS             41012..42139
                     /locus_tag="PKU88_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPX68817.1"
                     /translation="MNLGIDGRVITWHIGSGLATYTNNLLENLNKLKQLKNIFLFYPS
                     KNGTDYFEINDIPKNLLIGERRLDFWRNVYELKWDVNYPIDVFHNTVNGIGLPNCFKG
                     VKVITLHDLIPYIMPETVDRPHLDYTLKHTPYILDDISHIITVSRHSKSDIQKYFGIE
                     DDKITVTHLAADPIFRPLDRDNAKKVIFNKYGVDKKYVLYLGGFSQRKNIARLIRAFK
                     KVIIEKEEVINLLILGEHSRSFKALWKLTEELELCDYVKFLNFVPTADLPYFYNGAEL
                     FAYPSLYEGFGLPPLEAMQCGIPVITSNVSSIPEIVGDACLLTDPYSADDISDSMLTL
                     LTDGDEWHRYSLMGIEKAKEYSWFKTAAETLEVYEKSMKGI"
     gene            complement(42183..42716)
                     /locus_tag="PKU88_05715"
     CDS             complement(42183..42716)
                     /locus_tag="PKU88_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPX68818.1"
                     /translation="MDLKGSKTEKNLWEAFAGESQARNKYTYFASAAKKEGYEQISGI
                     FQETADNEKEHAKLIFKFLCGIGNTAENLKHAAEGENYEWSSMYKEFEKTAIEEGFKD
                     IAEFFGKVALVEKEHEERYLALLKNLEEEKVFKREEQTTWRCRNCGYIHTGAEAPEIC
                     PTCKHPRAYFEIAAFNY"
     gene            complement(42793..43332)
                     /locus_tag="PKU88_05720"
     CDS             complement(42793..43332)
                     /locus_tag="PKU88_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HPX68819.1"
                     /translation="MKYTILDFEAMIESVRKDGRIHNYEIQELKVYENYLYYFRKYVD
                     HKYHEWVEYNLFPRENIGIYKLQQSAKLQFNPSEVYKYYVDMVSVSKEGQFWKAKDLY
                     IDFIVKGDGRYYVVDVDEFNDAICRKELKENDVKNALNGLNNVLKGYYESNDLECYIQ
                     SLKEKYGGMGKLLLSKKTA"
     gene            43575..43765
                     /gene="ssrS"
                     /locus_tag="PKU88_05725"
     ncRNA           43575..43765
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKU88_05725"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(43819..45330)
                     /gene="malQ"
                     /locus_tag="PKU88_05730"
     CDS             complement(43819..45330)
                     /gene="malQ"
                     /locus_tag="PKU88_05730"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPX68820.1"
                     /translation="MKFGRESGILLHPTSLPSKFGIGELGSEAYGFADFLARSRQKLW
                     QILPLGPIGYGESPYQCFSAFAGNTMLISIERLIEDKLLLQEDIGPLPDFDEGLVEFE
                     KVRAFKSNLFRKAFKRFEAAGAGEDYDRFLRENDYWLNEFSFFMALKEYFGGAAWNLW
                     DKEIAFRDSKAMIYYRDKLSREIEYQSFLQYVFFSQWRLLKEYVNKQGIRIIGDLPLF
                     ISYDSSDAWSQSYLFQLDEEGNPLKTAGVPPDYFSETGQFWGNPHYRWNEMEKDGFKW
                     WRDRLDLLFQMADIIRIDHFRGFESYWEIPAGEKTAQKGCWVKAPGRKLFQTLRAHRK
                     DMHIIAEDLGFITKEVEELKNEFGFPGMKILQFIFNGPLEEELLPHNFEENSVVYTGT
                     HDNDTTTGWYLKMKIANPKADYEMKDYFHISGEIGEKAMCWTLIEAALSSPANTAIIP
                     MQDLLCLGSEARMNIPNTIGGRNWGLRLRKGMLTTELEEKLASLSELYGRNKN"
     gene            complement(45343..45843)
                     /locus_tag="PKU88_05735"
     CDS             complement(45343..45843)
                     /locus_tag="PKU88_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPX68821.1"
                     /translation="MQDNSRIVSADRDKFYKIMITRLEGLLSSESDWLANLSNAAALL
                     YTNLEDINWAGFYLMKKEELVLGPFQGKTACTRIKPGKGVCGTAAAEMRTQLVPDVHL
                     FPGHIACDSASNSEIVVPLIKDGTVFGVLDIDSPIKNRFDELDAKYLQLFADKLNKYI
                     DWSQIS"
BASE COUNT        12293 a        11319 c         7870 g        14459 t
ORIGIN      
        1 actgttattt cttattcaga gtatgcacaa acacgattcc cgcgaaacat aaatctattg
       61 aaataatgga gcaataattg tcaacagaaa ggctatgaca actaatatgc tgattaccgc
      121 aaccactttc cttttcacaa aatcaccccc gcaatttcat ataatctgct ccgccgttat
      181 gttcaaaagg cagacttatg tctgccgtta tttttatact tctatttctt ttctgagctt
      241 gcgccgatga gtttcgtatc caggatcacc ctttttccat ccctttctgc tcttttcgat
      301 attggcaaca aaactatcgt taataatgat ttcgtttttt gccaggcaga aatatgtggc
      361 ggtgttctgt ttacaatttt cgcaatgcat gcagttcata ttctacaatt gacctccagt
      421 tcttcattca aaatactcaa attccaaatc cagcatccta attgtatcac cgtctttaac
      481 acctaactgt ctcagctcat caacgatacc cctctttcta agtacctttt gaaagtactt
      541 gatagagtca ttatcatcca ggttaactga tgcaagcagt tgttcaatca gcttcccttc
      601 cacgataaat actccatttt cctttctggc agtaaatggc ttatcctctg tattaatatg
      661 atacatcaaa tctacgattt cttcaaccgg ttcttcatct tttgtacttt caagcaggtt
      721 aaaaactcta tccataagcg gtgcaatacc atccctggtt gccgccgaaa ttgcaaacac
      781 ctcatatccc ctcctgctaa gcgtctctgc caccctggca taattctccc tcgcagcagg
      841 aatatccatc ttgttgcagg caacaacctg cggcttgttc gcaagcttct cgctgtacag
      901 cctcaattcc tcgttaattt tatcaaaatc ctccagagga tctctcccct caagtcctga
      961 agcgtccagc agatgtatca gcacttttgt cctttctaca tgcctcaaaa aatcatgccc
     1021 caatcccact ccgctgtggg ctccttcgat aagtccggga atatctgcaa tcacaaagct
     1081 tctggcttct cccagatcca ctacccccaa attaggtaca agagttgtaa aatgataatt
     1141 cgcaattttg ggttttgcgg aagttactac agaaaggaat gttgatttcc ccacattcgg
     1201 aaatcctata agtccaacat cggccaaaag cttcagctcc agctcgatcc atctttccat
     1261 tcccggctcc cccggttggg caaacctggg tgcttgcctt gtgggagtgg caaaatgctg
     1321 atttcctttt ccgcccctgc ccccaagtgc aacaattgcc ttgtccccgg ggttcttcaa
     1381 atctccgaga agaagtccgg attcggcatc tctgagaatg gtgcctgcag gtacctttat
     1441 gtacaggtca tcagcactct tgccggatga attattggtt cctcccattt ctccgttttc
     1501 ggcaatatgc tttctcttat atctgaaatc catcagggtt ctcatgtttt cgtctgctat
     1561 gaagacaatg tctccccctt ttcctccgtc ccctccatcc ggtccgccat ttggcacata
     1621 cttctccctg cggaaagata ccctgccgtc tcctccgttt cccgctttta catatacttt
     1681 tgccttatct acaaacatat tattctcctt tgttgacctt ggaaatactc aaaatgaagg
     1741 ttgttccgtc cccaagcact tttccgtcaa gcttaacgtc gctgcagtac tccattatct
     1801 tatcgaatcc caaaacacct cttgctgctt ttttccaata aatcaccttc tcgggaaaga
     1861 taaaatcaac aggcccgaac agtcctttcc ttatctccct gcagtaaatt tccatcagaa
     1921 atccttctga aatttctttc aggttatata taatatgggc atttttcata cccttttcat
     1981 caagcacata taaaaatccc attatcacat ggtctacaac atatacaaca ggaacctcgt
     2041 caaggatcct tacagcagaa tcaaagctta cctcaacgct gacctcaagc ttaagatctg
     2101 cgctctccgc ttcttttacc ttattgtaaa gtaaatcggc cagttttata catttacagt
     2161 tatatagcct gcctatgttt tccatccgct tgcagtattc agaaatgtgt tccagcacct
     2221 tgtctggttt ctttagctga gtatagccgt acaatacttg aagcaggttg aaaaagtcgt
     2281 ggcgctgttc gcttaaatgc tctttcagac tctctatata acgctccttc tccgactcaa
     2341 tgtcaggaac atcatatatt ccgctattct ttatgctcat attcatctca tctcctatac
     2401 tgcaaaaatg ctaaaaaagg tacagcctgg ccgtaccttc agtaattcta tgctattatt
     2461 gcaccggata cacacttgcc tgcttcttgt ctctgccttt acgttcaaat ttgacaacac
     2521 cctcaataag cgcaaataat gtgtcatcct taccaatccc cacatttatg cctggatgga
     2581 tttttgttcc tctctgtctt actattatat ttccggcaag cacagactgc ccgtccgcct
     2641 tttttgttcc cagtctctgt gcatgggagt ctctgccgtt tcttgagctg cccacacctt
     2701 ttttatgtgc aaacaactga aggttcatct tgaacatatc tacacctcct cttcaactat
     2761 atcaatgtgt cttttatagc tgtccttaat actcttaaat ccttcataca tggtttcaag
     2821 tatcgcatca cacattatgc gctttctgtc tgatcctgca ccggcaatac tgcaatcaag
     2881 gtatccctcc tcaatcctgt agtgcacctc cgcttcggca actttcagca gccccaggag
     2941 agccgtttgg ccaagggcag aaaccgcact gcagactata tcggtgccat gagtaccgta
     3001 gcctgaatgc cccgatatct tgaaacccgt tattttgccc tctcgggcct tgtagatcac
     3061 aatcttaatc aaaaaaaaca actccgttgc tttttccgct tttatgcgtt tattttttcg
     3121 atcttaacct ttgtaaatgg ctgtctgtga ccctgcttcc tcctataatc cttcttcggc
     3181 ttatatttga atacaattac cttctttgct ttgccattcc caacaactgt tccttcgacc
     3241 tttgcacctg caactacagg attcccgacc tgaagctcac cgttatcgcc tacagcaagt
     3301 acttcttcaa atacaacctt ttcaccggat tccgcatcaa gcttttctac cgcaagtaca
     3361 tcaccggctt taaccatata ttgcttaccg ccggtcttta ttattgcata cactaggcta
     3421 cacctcctca taaataagtc tcgccgaatt ccaggcatcc tcattgcatt agagaattta
     3481 aacccgattc gtgcggatac gcctacgcca atatatgtta tcatatagca acatttttgt
     3541 caattaaatt tccctgcagc cctttttgcg aagctccgta atttccgaca tgcctatgcc
     3601 tttcacaata tagccttccc tgtgaagctc tgccgaagtc ctgacaaata caggtttatt
     3661 aaagctctta cccaactcaa acatgtcctc gtctatataa tagtttatca attcttccac
     3721 atccggatga atctcaagaa aaacagccat ggcaccggta ttaaaaaaca agctgtttac
     3781 ttccttctca attttcttga caattgtctc actggataaa atccttccct ctcctttgca
     3841 gtaagggcat ggtgccatca gtatcttttc tatacgctgc ctcacctttt ttcgggtcat
     3901 ttcaaccagg cccagctgag ttattccaag tacgttggtt cttgtcctgt cttttttcaa
     3961 tgcatttttt agagcctcaa gaactgtact ttcgtggatt tcatccgcca tgtcaatgaa
     4021 atcaattatt atgatgcctc ctatatctct taagcgcagc tgctttgcta tctcttttgc
     4081 tgcctcaata tttgtcttaa gcactgtatc cttcaagtcc accgtaccga catactttcc
     4141 tgtattcaca tctatggatg taagtgcttc agtctggtct atgacaatgt atcctccgca
     4201 tttaagccag accttgcgat tgattatcct gcttatctga ggctctgcct gataatattc
     4261 aaatatatca tagcttttat tgtaatattc aactttgttt ttaaaatgtg atgaaataat
     4321 tgttacaagc tcccgaacct tctcatagtg ttccttattg ttgattacaa gcctgtcaat
     4381 atccttggtg aacatatctc tgacagtcct gtatattatg ttcatatcct tatgtacaat
     4441 tcttggggcg gcgccggttt tttgcttttg ttttatatta tcccaaagct tcaataaaaa
     4501 atttatatca ttgcttaaat ccgcttcggt gcaatcctct gcagcagttc tgattatcag
     4561 tcccattccc ttgggtttca gctcttctgc cagctttttc agcttttccc taatttcttc
     4621 atcctctatg cgccttgata ttcctatata ttcaacagtg ggcatcaata cagcgtatct
     4681 tcccggcaga gtaatatgtg ttgttactct ggctcctttt gaatctatag gctccttcat
     4741 aatctgaacc attatttcct gcccggcctt tagaagctca tctatctgat aatccttata
     4801 ttgcgcagaa ctgacatact cctcatcctc atcaaaataa gtatttggaa gcgcatcctt
     4861 tacatataaa aatgcattct tatccaaacc aatattgata aaggcggctt gcattccggg
     4921 aagcacattt tccacccttc ccttgtatat attgccggta atcctttctt cgctctgcct
     4981 ttcaatatat atctccgtca gttccttgtt ttccagcaaa gcaacacggg tttgcctgga
     5041 accgacatca ataataatct ctttcataaa tcgcgctcct tcatccttcg attcatggtt
     5101 ttttatgctt ctgcgctttc atattcaagc aaatccaaaa gcttgccgtt ttttaacccg
     5161 tatacttgtt ccctgtttat cctgatgctt tgaatatcat atcccgtaaa ttccttaaag
     5221 gccttaatta ataatccggg tttaaggttc cctctgcttc cgcttagaag aagacagtca
     5281 attgtgtatg tatcttcttt cgactcaatc agcttcatac tgactaacag gggttttata
     5341 tcaatctctt tgagttcaaa gcccttcttg ggctgttcct tcattatctt tatttccttt
     5401 tgtgctgcaa attcttcaat tttctttgtc agctcatcag aagatacgtt ttccataaaa
     5461 attctgatgc gatatcttga atgagagaca accgacatgg cactcttgtg gttttcccta
     5521 atctcaatac cgtccaatat ccttattcca tccggcagcg agtcattcag gcgcctctgt
     5581 atctccctga catccattgg ctccgcaagc tttaaatcca catattcggc attgctggtg
     5641 acacccaggg ataatggagc cccaaaggaa atctccatgt gaggattgaa accgctgctg
     5701 taggatatgg gtatgcctgt tcttctcaat gctctttgaa atactctcat aagatccagg
     5761 tgggatatat atttcacttc attacccttg gtgaatttaa gtcttatatg catcatttac
     5821 agacaccgcc ttcctgaagc agattaatac cgcagccggt gcaggcagtc ctgcaatctc
     5881 tcgtcaattc tcccttaagg gatttcttgt actcccccct caaatacttc ttagtcactc
     5941 cgacatcaat atgatcccag gggagtactt catccagctc tcttcgccta gcggcataag
     6001 aatccggatc agctcccgta tctttaaaag cctgcaacca ggcctcgaaa tcgaaatact
     6061 ggtcccaacc gtcaaatttg catcccagtt cccatgctct tatgagtaca tcacagagcc
     6121 tcctgtctcc ccgtgcaaat accgcttcca tgtagctgac ataaggctca tgccagttaa
     6181 aggttatctg ctttttctta atagcctttt gcaggtacct ctgcttttct ataagagtat
     6241 tgatgggatc ctgaggctcc cactgaaagg gagtaaaagg cttgggaaca aaggaggaag
     6301 cacttgcggt tacattaagg ccctttcctc ttttctcctt aggtacctgg taatacctgt
     6361 ccactacctt gaatgtcaaa tctgcgatac cctgaatgtc ttccattgtc tctccgggaa
     6421 ggcctatcat ataatacagc ttgacgctgc tgtagcctgt ttcaaatgca gcacccaccg
     6481 agttaagcag atcctcttcg tctacacctt tgttgatcac attccgcatt ctctgagtac
     6541 cggcttcagg cgcaaaagta aggccgcttt ttcttacttt ctgaacctcc tggaccagtt
     6601 tcagagaaaa agaatcaatt cgaagagagg gaagagacaa tccgattctg ctgctcttat
     6661 atctggaaac cagccgttcc accaattcat taagctttga gaagtcactg gttgacaagg
     6721 aggacaatga aatctcctca tagccggaac tctgtatcaa cttgtccgct atttccaaca
     6781 gctcctccac ttctctctcc cttaccggcc tgtaaatcat accggcctgg caaaaacggc
     6841 atcctcttgt acagcctctg aatatttcaa gcattatcct gtcatgtaca atatcaatga
     6901 agggcactac tattttgtca ggaaaatatg aacccttcag gttctttatc acccttttcc
     6961 tgacttttcc ggggatatcc tcttttattg gctttattga ctttatggtt gaatcttcat
     7021 tatattcaac gttgtaaaac ttcggtacat atactcctct gacagaggct gccatcttca
     7081 ggaactcttc tctggtgcct tttgagtcct tccacttcct gtaatgctcc atgacctcca
     7141 gcagctgctc ctcaccttct ccaagcatga agaaatccac aatgtcataa agaggttcgg
     7201 gattatatgc acaaggccct cctgcaacaa caaaaggatg gccttcattt ctttcgccgg
     7261 atcttttggg tattccggcc aaatccaaca tattcacaat gtttgtatag ctcatctcat
     7321 attgaagggt aaaacccaca aaatcaaagc tgtcgataaa atccctgctt tccagcccaa
     7381 aaagctttat gccggttttt ctcatcactt cttccatatc gggccacggg gcaaaaaccc
     7441 tctcacagaa aatatcctgc tgatcattca acaggtgata caatatcttc attccaaggt
     7501 gcgacatacc aacctcataa acatcgggaa agcaaaatgc gaatcttata tcaaccgttt
     7561 caggattctt atgtatgctg ttccactccg ttccgatata tcttcccggc ttttccacct
     7621 tggacagcag ttcctcatat atgaactccc tggtgttatc atatttcatt tgttcctcct
     7681 atagtttctg cacacggctt aagaatgcga cataggtaac gggtaacact aaacacacag
     7741 atgtttcacg gaatcgctgc gcgactacac ggcaactatt atttgttata tatctacata
     7801 agaagatttc gatatttgca tcattatcaa taattatcta tgtatcgaaa tatttatccc
     7861 gccgtgcatt tagtggtttt tcgtgtcttc agtgttgcca aggcctcgaa gtcctgccct
     7921 aaatatcgta cctcgtaacc tcgtaaccta atttatatta ttacataatt gcagtataaa
     7981 attctactga aagtttatct atccgttaat tatttgtgca atttttccat catatccgta
     8041 ttgaagaagg ccttttatga tttcttcttc actcagaatc cttttatgcc tgccttttgc
     8101 atccgctact tccacgcagc actgggtcac agggctgaat ttgtttacta tctgtcttat
     8161 caggttatcc tcctgtactt ttataaacct ttttcttatt cgcccccggg ctatcatcgc
     8221 attcttggtg ttattccccg taaacaagta ataatacgag ctgtttttct cttcctttaa
     8281 agcaccaata tatatgaata cagccgcaat tataagcgcc ccgcttttgc ttccggctgc
     8341 cagcatgtga atattgaagc ccagaagcag aaaagctgct attttacctg ctgacgataa
     8401 aatttttgtt gcctgcctgt atcccatcag gaagcaaagc agattccttg caatcctgcc
     8461 tccgtccagc ggaattacag gcaaaaggtt gaacaggcaa attatcaggt tatatctgca
     8521 ggctgattcc aatacccctg aatactccct gaaaaaactg aatgtcaatg acaatacaaa
     8581 gctggtgaca ggccctgcag ctgaaaccac agcttctgca gcccctccca gctttgataa
     8641 ttcttccatt ctggatattc cgccaaaagg cataagctca acttcatata cctttattcc
     8701 tgacttttta gctgtcaata catgtccgaa ttcgtggagc aatacactgg caaacattat
     8761 caaggactgc tgcagccagc ctataatgca gctcacaaat aaaaaaagga taaaaaaaac
     8821 atttagtttt atcctcacag tgtgaacccc caatgccgcc ggttatactg ttcactgcac
     8881 ttccatcagc atttccattg ggctgacagg cttccctgcc tcttgtatct caaaatgcag
     8941 atgaggcccc gaggtaatac ccgtgtcccc tgtctcccct attatttctc cctgctgaac
     9001 cttttgtccc tctttaactg ttatcttgta gcagtgggca taaatcacat catatttgcc
     9061 gctccttata cttacatact ttccgtttgt ttcatcctct ccaactgttg ttaccgtacc
     9121 tgccgcggaa gacttaatag gtgtgccgat cgctgcatct atatctattc cggtatgcat
     9181 cctcactgtc ttgaacaccg ggtggattct ttcgccgtat tctgatgtta tttccccttc
     9241 cacaggaaat ataaagcttg gactgtcttc aaagctttct attccgactt gggcaactcc
     9301 aaggttctcc tttgcctccg gtattatatc tccgacattt ctgaatgtcc ccaatacctt
     9361 tgaaaaatcc atattccagt ttatcgttgt tcttataccg tcagatatcg tgtttgcaat
     9421 gccaatatta atattattca ctaacagtac tgctgctatg gctgcaagag atataaagga
     9481 ctgcatcaga aacctttctc cgaaattcct ttcaccatac cgccttgttt tgaaggccgc
     9541 accgagtttt ctatactcct ttacatacct tggcctggga atattctcaa aattattgtt
     9601 gtccatatat tctttccccc atagttcttt atagattaat atatttatat ttatactcca
     9661 atggaattat gtcccaaaaa ataaaaaaag gacacgggac acggggacgt ttctcctgtc
     9721 ccgctttcag cgggacagga gaaacgtccc cgtgtcccgt gtccttcctc tgtttctaaa
     9781 tattcacatt gcctttcatt ctttttatcg gtatattggc aaccaatgca ggtaccgttg
     9841 aatcatcaat ctcccttttt gtccttgtaa gcttgatttc aagccctgtt tcgtctattt
     9901 ccatataatc tgatattacc ttaatgagtt ctcccttaat catttcaaga aattgaggag
     9961 atacattggc cctgtcatgg atcaatacca attttaatct ttccttggct atatccttac
    10021 tgcttgtact tctgttaaac cacttgaaca aatccataac tcacaccccc tcttactgct
    10081 ttttcttatt caatccgaaa agttttgcca ccctcttgaa aaaaccgtcc tccgattcaa
    10141 gattcataaa gggaatatct tctcccagta ctctccttac aatattccta taggcttgtc
    10201 ccgccaggga ctgttctatt gcaatgacag gctctcctct gttggtggaa acgacaatca
    10261 aatcatcatc cggaaccacc cctaaaaggt caatggcgag tatatctatc atgtcatcaa
    10321 tgttcatcat atctccgcgt ttgaccatat ccattttgat cctgtttata ataagcattg
    10381 ggttccttaa tccgcttgct tcaagaagcc ctataatcct atccgcatct ctgacagccg
    10441 atacctcagg ggttgtaact actatcgcct tatttgcacc tgctattgca ttcttgaaac
    10501 cctgctcgat acctgcggga caatccaata ttacgtaatc aaactcctcg gcaagatctc
    10561 cggtgagttt ttgcatctgt tcgggattaa ccgcattctt gtcttttgtt tgcgctgccg
    10621 gaagcaggta caggttggga aaccgcttgt cctttatcag tgcctgtctc tgtctgcaaa
    10681 caccttccac aacatcaaca aggtcataaa ctattctgtt ttccaagccc atcacaacat
    10741 ccagattcct gagccctata tctgcatcaa ccagaacgac gctcttccct ttaagagcca
    10801 aaccggtgcc tatattagcc gtagtagttg ttttcccgac tccgcctttc cctgatgtaa
    10861 caacgatcac ttcacccatt atattacctc cataatttat tgtatcagca tatttatatg
    10921 atataaacta tctgttcttc aaatatggct caacaactat cattccttgt ttgaccaatg
    10981 caatctcagg attatccgac ttgctgccca ttccatcagg ggatcttgtt attatatctc
    11041 ctatcctcag ctgggaaggc tgcattttat atgcgacaat gaatgcccca tagtcgccgt
    11101 tggctcctgc ctgaataatc cctcttacgg ttcccattac aataacagat ccggctgatt
    11161 tgatgtgggc tccgggattc acatccccaa tcaccactac gtccccttta tattgaatca
    11221 actggcccga cctgagtgtt gccctgacta tcagcgcttc tcccttatgc acattgtcaa
    11281 aaggaagctg ttccagtatt tccggctttt tggctcctcg atgctcttcg tgtacaggat
    11341 agtccttatc gttgtaactt cctattactg tcatgccata ttcctgctcc atgatttcct
    11401 taagctcatc aaactcatcc tgagtaagct tctttccctt aaagttcata atatttgcgc
    11461 cttcaaaaaa acgcttggaa ggtttgattt tcttttccag attatatttt atggtgctca
    11521 aatccatttc ttctttgagt attatatata atccgtcttt tgtacctttg aatatcactg
    11581 cattttccgc catggcagta ccccctgatt ccggatatat cgccctgata tacatagtct
    11641 aatatttctt cataattgtg ataaatcctt cttcccaatg aaaaaatgca tccctacggc
    11701 aatatttcat tgtcaatatg catattatcc ttagtcgcct caggagccag ataagcttca
    11761 attatttccc tggcaaccgg tgcgggatag tttccgtgac caccctgggt tatcatcacc
    11821 acaacggcaa tctcgggttt ttcgtaggga gcaaaggcgg caaaccacgc atgggcatca
    11881 tagcctttcc ctacttccgc agttcccgtt ttgcctgcta taataacctt gctgcctgta
    11941 aaagcacttc tcgctgttcc gccggcttca ttggtaacag catacattcc cctctttatc
    12001 gcttcaagat tggcgggaga aatatcattc ttttccgcca cttccggctt tttttccagc
    12061 tttacgttac catcatagtc tatgacctta tctaccaggt aaggtctata ccttgtacct
    12121 ccattcacta tagacgcaac agcatttgca agctgaagag gagtaacacc gttcattccc
    12181 tgtccgattg ttgcgttcag cacatctccg ggctgatact tgctgtccct tatgtatctc
    12241 caaacccgtt caatcgcctt tgaatcatta ataccaagtt ctttgagcct tttccttact
    12301 ttgctttctc ccggattatt atccaagtat ccttttattt catcacggac attcttatca
    12361 tttatctgca gattatttac aagatagttg tcaagatatc ttctgaacag ctctttttta
    12421 ttcgtcgggc cttcaacggt tcccttatac tcagagagca gctctatacc cgttttctcg
    12481 ccgaagccga atttcttcgc atattcttca aatacttctc cccccattct tctgcctacc
    12541 tcgaaaaaga aatagttgca tgaattctta attgcctggg atacattctg actgtaatgg
    12601 ggaatgcctg tggatttaaa tattgaacag gacggagata ccccgggtat agctgtataa
    12661 tagcctttat cgtatatcgt ttccgaagga gttattacct tgctttccaa ccctgccacg
    12721 gcactaacca gcttcatggt tgatccgggg gaaaagatag atgaaattgc attattcatc
    12781 agcggcttag gtgcatacac atccttgctt ttaggctgca ggttcttcca atcctcactg
    12841 ctgattcctg ccgcaaaaag attcggatca aatctgggtt cacttgccag ggcaagaatc
    12901 tttcccgtat tcacatctat tgctaccgca gctccggagg ttgcattagg atatggctta
    12961 cccttcacaa tgccatttct tatattctcc atggttcttg ccaatgattc ctccgctgtt
    13021 ttctgaagct tggcgtcaat agtcagaaaa accgtatcac cgggaacagg atctatttca
    13081 tttattgtat ttatcagcgt cccgtttaca tctacctcta tctgcctgga accatccttg
    13141 cccttaagat attcttccat ggaattctct atgccggact ttccaatcag atcctgggga
    13201 gtgtatcctt tacttgccaa ctcctcaagt tcacttccga tcatacccat atagccgata
    13261 atgtggctgg caaaatccct tcccgcatat gatctgattg gcttctggat tatctccact
    13321 ccgggaagaa aaatcttgct ctcttctatc tgagcccttg tttccatctt gatatttgcg
    13381 gctatttcta ccggttcata tgctttatac ctgttctgtc cgaccaggta cttgactgcc
    13441 agtatttttt ttgccttttc atcatcatag atatctctgg ggattttata tttgctgcag
    13501 agtacatcaa atatctccga cgcacttttc tcaggcccga agccatggct ttgcttccat
    13561 tttatctcag acttgcggaa agaaaattca aactcattga tgtcaaaagg cagttcaacg
    13621 cttttttcat ccctcataat ttcataagct tcaagatcaa tggtatccgc cggaatctca
    13681 taatcaaatc taagcttcat aaaagcgtcc tttgcagatg cactttccgg aataccgtac
    13741 ttcttcttcc agttgatttc atcatcctga taagaaaacc ttatgggatt aataagtatt
    13801 ggtatttcat ccttgtaggt gtctccgttc tgttcaatta tattaattac agacagtgca
    13861 atatcgttca atgtatcctg gggtacatcc gttttcatta tattaacgga atagctctgc
    13921 ttgtttcctg caataagccg cccgaacttg tctacaatat gccctctggg tgcagtcact
    13981 gaaatattcc ttgtcctcaa ggtctcagac tttttccggt aatattctcc ttttaccagc
    14041 tgcaagtctg ccagcctgaa cgccataacc aaaaaaacca agactgttac cgccctgatg
    14101 atatcaagcc tattactaaa atatttctta aacatattat catctactcc ctataacctg
    14161 catctcatgt ccggtgtcta tcagtatatc cttctgtcca tgaaagaggt ttcgtccaac
    14221 ttatatataa acctgtaaac tatcgaaccc gctatcgcat tatataatgc ctgaggcaga
    14281 atcacctgca gcagcatata tatgtatgaa ctccctgcgc ttggaaaatc attggaaaaa
    14341 tatgaagcta gcaaaaagct gtgctgaaga atgataattg caataaggtt aaaaatgaaa
    14401 gggggtataa agctgtcctt aaatactttt tcatgagctt gtcctattat atatccggtc
    14461 accatataag aaagggcgtt gatgcctatg actctggcat acataacatc aatcagcagt
    14521 cctattccaa ggcctataaa agtgccgaag gagcttcccc tcataattgc atatgctact
    14581 ataatcatta ttgaaaagtc cggctttata ccattaattc gcgtgcattg aaataatgtc
    14641 gattcaagta tgacattaaa aacagccaat atgcatataa tcgctattct cacaagcaat
    14701 ccctcacatt ttacttggag gctttgatta caaatacctc ttcaagcctt ttaaagtcta
    14761 ctgatggctc gataattacc accttctgca gctttttttc ctgtttctcc acactcttca
    14821 ctttgcctat gtgcaggcct tttggcaaag tgctgtactc cgaagtaata atatcgtcac
    14881 ctttgactat gtccgcctca agctccatta ttgctataac atcagaatcc gcgctgcctg
    14941 atacaattcc catatccctt gtcctgttca caattatgct tatagagctt ctttcatcaa
    15001 taatagccat aagctttgaa taatttgcac ctgtctcaat aatttttcct acaagataac
    15061 catggccgac tattacggca tcattgactt gaattccatc cccggacccc ttatcaattg
    15121 tatatacatc ataccagttt ccgggatctc ttccggtaac attggctcca atatattcaa
    15181 actgagtatt tgtatccttg aattccagaa gactccgcag cctgccgttt tccaatgcca
    15241 tttgaataag ctgcctgttt tgcgccttca gcttctcgac ctccgcctta agattttcat
    15301 tttccgtttt aaggtctttc aaatcgtata agctcttgaa ggtattctta aaaaactccc
    15361 ccgaagagta gaatactctc tgaacaggag ttattatcct tccaaaaata ccatcaatca
    15421 cacccggcct tgtcctacct tgagatgtaa cagcaattaa tgatattaat agtattgata
    15481 ttaatacaac agaaataagg agcttattcc taattttcat attactaaaa cctcacaaaa
    15541 ccttatctcc tatgtttccc ggaatatgat ttgcttattt cgtcatattg ctccaatgct
    15601 ttgccggttc caatcgccac acaatccaac ggattctcgg ctacctgcac cggcattcct
    15661 gtttcgtgct tcaccagaat atccaatccg tgcagaagcg ctcctcctcc ggaaagcatt
    15721 atccctctgt ccattatatc cgcagccagc tcagggggtg tcttctccag agtactctta
    15781 atgccgtcca gaattattgc aatgggttct cttaaagctt caagaatttc ctcacttgta
    15841 acctttattg ttttgggcaa acccgtaacc aggtcccttc ctttaatgtc cattgaacct
    15901 tctgctgtat taggataagc agatcctata acaattttta tctcctcgcc ggtacgctct
    15961 ccgattgcca aattatacat tctctttata taatgtacaa ttgcttcatc ctgtttgtct
    16021 ccggccgccc ttatggattt gctcgttaca atccctccca gggaaatcac agctatttcg
    16081 gtagtgcctc cgcccatatt gactatcatg cttccggttg gctctccgac gggtaaaccg
    16141 gctcctattg ccgctgccat cggctcttca ataagtcttg caatacgtgc gcctgcctgc
    16201 attgctgctt cttcaacagc tcttctttct acttccgtaa tacctgaagg aacacttaca
    16261 agaaccctgg gctttcctac cggggaacct gcataggctc ttactatgaa gtactttatc
    16321 attctgtggg taacattgaa gtcggcaatt actccatccc tcataggcct tattgctata
    16381 atattaccgg gggtcctgcc aatcattttt tttgcttcgt ctcctacagc cattacagca
    16441 ttggtatcaa gcttcatagc aacaacagac ggctctctta caacaattcc cttacccctt
    16501 acatatacca gtgtatttgc ggtacccaaa tctataccaa tatcctttga caaaaaatta
    16561 aacaagccca taatatatgt cctccttaac aaaatacaca catcaattgt acattagctt
    16621 ttactgtata taggcaaaaa tagttatata aaccttttat atatgtttta caaacggaaa
    16681 acaagttatt cacttttgac acctgaaaaa cactagaata gtcctttttc tttcatactg
    16741 acatatttgc cgtctcctat gataatatgg tcaaccagtt caataccgat aagcttcccg
    16801 gcttcagcaa ttcttgcggt tattgatatg tcttcgctgc tgggggccgg atcaccgctg
    16861 ggatgattat gcaccatgat tatactggcg cttgatttcc ttatcgccgg gttaaaaacc
    16921 tcccgcggat gtacaatgga agcattcagg cttcctactg agatttcctc cactgatatc
    16981 agattgcatt ttacattaag caatacaagc ttcatgtgtt ccttcttgag atatctcatc
    17041 tcctccataa gcaggcctgc aacatcctgg gggcatttca gcggcttgtc cgctccgggc
    17101 tcaaaagcag ccaggcgttt gcccagctct atcgctgcct tgatctgggc agcttttgct
    17161 ttcccgattc cattgatagc gctcagctgt tccacattac tgtccgccag gtatctcagc
    17221 ccctgttcct gtaccagtat tctatgggcc aaagatattg cgctttcctc cctggtcccg
    17281 gtccggagca atatggccag aagctccgca ttggaaagta ctgaagcccc atgtcttgca
    17341 aggcgctctc tgggtctttc ctcctccgga agatttttta ttgtggcatg acctctctgc
    17401 atcctttact cctccatggt ataattaaat cctccaggga ttatcaaatc atgcaggcta
    17461 aataagcttt actccgaagt gctccagcat ctttctcagc cggaatatcg gaagccctac
    17521 gacattgaaa taatcaccct gaatcccttc aacaaatagt gaacccaaac cctgtatagc
    17581 atatgctccc gctttgtctt caggttcacc ggtgtttata tatgctgtta tctcagcact
    17641 gctcaattcc ttgatttgta cccttgtgct ctcatgctca gtcaggcttt ctcccgttgc
    17701 agtattaata atgctgatgc ctgatatcac aaggtgctgc ttcccgctaa gctttgtcag
    17761 catgcagaaa gcttcctccc tgtttcttgg ctttcccatt atttcatcac aatacacgat
    17821 tgtgtctgaa ccgattacca atgcatctcc ctttacggac ttcgccacat cctttgcttt
    17881 catataagca aaatgctcaa caagttttga agccggcaat tcagctatac tttcttcaaa
    17941 acttgatggt atgacctcaa acttaatgcc tgtctgagac agcagttctt tccgtcttgg
    18001 ggaatttgaa gccagtatta ttctcattta tctcacctgt actataggtt ttacactaaa
    18061 gctttctgta tatataaatt gcaatcacaa ttcccagtat gctgaacata tttatattga
    18121 agtcaaaacc aaaggtaagt ctaagtatgt ggaaattcca ctcatgagcg gaaaccccca
    18181 ggttgtaccc atacttaagt atcggaaagt atttccccag cagttcaccc aaaaaaccgc
    18241 ccacgaccaa tccgcataca accatcagaa gcattatcca cccgtttctg ccgccttctc
    18301 ttctcaacac attcatctcc tttattatta acttctgcat atgtcagcaa tacgtgaatt
    18361 ccttatgttt tagtttaaca ttttaaatac cggacttcaa cttttttgga attcatttga
    18421 catctaatat cgacatttta tataattcgt aaagctaaag aaaaaacaga ggtaaagcaa
    18481 aaaagaagaa tatcaacatt cttcttccag ttacgaggtt acggaaaggc aaataccgac
    18541 atggggaaga tatcttcgag gaaaccttcc acctgacccc ggaacaggaa tacctcatag
    18601 gcaaaataac cagatggacc cgcaaagatg ggacccacgc cagtgatttt gctggctttg
    18661 gaaggtgagg agaacatact tcccctacag tcggagttat tcacaaatct attatggctt
    18721 attattcttt tatttcattt caatggcatc cttggggcac ttggcaaagc attggccgca
    18781 gcctacgcat ttatcgtcca gcaccttatg cttacccttc aattctccct caattgcctc
    18841 aaaattgcaa ttcttcgaac agattgtgca gcctatgcac ttgtcctcaa ttatgaaggc
    18901 tttttttctg tctgcgaaat tggcatatat ggtttttgta ggacatactt cagcgcacat
    18961 catacaattt acacatttgc tgtaatctat cctcgctaca ttgttttcaa cctttattgc
    19021 atcaaatttg catgctttca cacatttttg gcaagcaatg cagccgattt tgcaggcttg
    19081 cattgtagct ttgcctttat cctgattcct gcaaagcact ctgacctttt ttgatacggg
    19141 aaccagctcg ataatcccct tagggcatac cgctatgcac tttttgcagg ctgtacattt
    19201 ttcttcatca actacagcaa taccgttaac tatatgaata gcatcaaagg gacattccct
    19261 tacgcaggtg ccaagtccgg tgcagccata ccgacagctt ttggaacccc ctgcaagcat
    19321 ttctgctgcc ttgcaatcct ttatgccttg atattcatat ttttctgctg cattgttact
    19381 gtcaccgcag cagattactc ttgctacctg acgttcctcg gaacctgctt caacacccat
    19441 aatcactgca agctttgccg caagctcaga ccctcctaca gggcattggg aaatcggcgc
    19501 gtttccgctt gcaatagcct cggccaatgc atcacaaccg gcaaatccgc atccgccgca
    19561 gttggcgcca ggtaatacat ctctgatttc agtaaccttg ggattaacct caacagcaaa
    19621 cttctttgat gcatatgcca gcacaatacc gaaaaaaact gccattcccc ccagaactat
    19681 tacaggcaat cctatactat taaacaaggt agtcacctct actttcttcc ataactaata
    19741 ctacagcaat ccgctgaaca ggccgctgaa tccgaggaaa gctattgaaa acaggctcgc
    19801 tgctatcatt gctatgggga agccttccag ggcttttggt ataggagcca attcaagtct
    19861 ttccctgaca cctgcaaaaa gtactattgc cagtgaaaaa cccaaacctg cgcctacgcc
    19921 gttaattaat gactcaatca aattatagct tttgtcaata ttcagtatcg caacacccaa
    19981 aactgcgcag ttggtagtaa taagcggcag atatatgcca agtgcctgat acagtgttgg
    20041 gcttgtcttt ttgacaacca tctcaacaaa ctgcacaagg cctgcaataa caagtatgaa
    20101 ggctatagtc tgcatatatc ctatacccat ggggtcaagt acaaaaattt gcaccgccca
    20161 gcatattaca gatgccaaac ccattacaaa agtaacggct atactcattc ccactgctgt
    20221 gtctatcttc tttgacactc ccaggaacgg gcatataccc aagaactgag agaggacaaa
    20281 gttatttatg aatattgcac tgataataat taatcctaat ttcatctata tcctcctcct
    20341 taactttttc tcaggctggt aattttattg aatgccgcaa ttgaaagccc aaataccagg
    20401 aagcccccgg gaggcagtat cattatcaaa aacggctcaa aagcttcacc aaataatggc
    20461 attcccagga atgtgcctgc accaagaatt tctctgactg agcttattat tgtaagagca
    20521 acagtaaatc cgatacccat gcccaaaccg tccataatgg aattggcaac agggtttttt
    20581 gctgcaaatg cttcagctct tgcaataatt atacagttaa caacaataag aggaatatat
    20641 atcccgaggg atttgtccag tgcaggcgaa aatgcctgta taagcatctg aactattgta
    20701 gtaaacgttg caattacaac aatatatatc ggaattctaa cttcatttgg tactattttc
    20761 ttaatcagcg aaatgataag gtttgagcct ataagaactg cagcggccgc aatacccata
    20821 cctatgccgt taaatacagc agttgtaact gcaagagtag ggcatgttcc gagtaccaac
    20881 ctgaatgtcg gattctcttt tataaggccg ttgctgaaat ccttccaaag attcattttg
    20941 ttcacctcca aattttttct atttgcctga taattttttt gccgcctcaa gggcctggtt
    21001 caccccgtcc gttactgccc ttgatgtaat tgtcgcacct gatactgcgg caatttcatt
    21061 atcctttgga gtaccgctct taataacatt tattgtatta tcccaagcct ttcctttaaa
    21121 ctgattctgg aatttaggag ttgcggaatt tttcccaagt cccggagttt cagtattatt
    21181 tcccactttt attccctgaa tgatcccatc cgtaccaata cctacaacta catctatagt
    21241 tcctccaaag cctttcggaa ctgcctttac agcgtagccc acaacatttc ctccggcttt
    21301 tgctgtgtat atttctgtta caaagttata ctttccattg gatttaattt cagaaaatgc
    21361 attgtcatcc aaaatttcaa atgcgtcagc atcaggaagc acttcttttc ttgcttcatc
    21421 gtttgctttt ataatctgct cctgtatcct gtcgtaggtt attgcatttg tgtagcccag
    21481 agatgcagcc ataacaacgc ataccaaaag caaaattccg gctaatctga tataatcacg
    21541 cacttttctt cacctccccg aatttcctgg gcattgtgta tttatcgata aggggtgttg
    21601 ccaggttcat cagaagtatt gaatatgaaa caccctccgg atagccgccg taaaacctga
    21661 tcactgatgt aaggaaacca cagcctattc cgaatattat ctgccctttt ggagtaattg
    21721 gagatgttga ataatctgtt gccatgaata ttgcaccaag gaaaagtcct cctgccaata
    21781 catggtaaat tgctgcagca aagtcaaatc ttccgaaaag taatgtaaac accgctaccg
    21841 cagctatata tgaaacaggt attctaatac ttattactct tctatatacc aggtaagctc
    21901 cgccaagtat aagtgccagt gcagatgtct cgcctatgca tcctccgata tttccgataa
    21961 acaaatccca taagcccggt aatgtttgtc ccacagcttc tacaccctct tcccagtgag
    22021 tagcccctga aagagcatct gctcccatct ttacaagtgc aagaggagtt gctgtagaaa
    22081 ctgcatccgt tcccggagca acccaggctg tcatccttac aggccatgaa gcaagaagca
    22141 tggctctcgc cgccaaagcg gggttaataa aattataccc taatcctccg aagcactgtt
    22201 tggcaatcgc tattgcaaat attgaaccaa cggcagccat ccaaagagga agtgttacag
    22261 gtacgttgaa tgcaataagt ataccggtaa caacggcact caaatcatta actgtaacag
    22321 gctttcccaa tatcttctgc atgacggctt cagttgttac cgcaaatact acagcgacag
    22381 ccgtaagagt caatgcgttt aatccaaagt aaaatacacc ggccaaaagc gcaggcataa
    22441 gtgcaattat cacatccagc ataatccttt gggttgtctc cccggatctt atatgcggag
    22501 atgatgatac taaaaatctg ctattttcca tttcttcccc tcccatcact tagactgggc
    22561 ttttttacgc ctagccagta tttctcgttt tgccaccctg attgaatcaa caagaggcct
    22621 cttagcaggg catacaaaag aacaagctcc gcattcaatg caatccatag catttaaagc
    22681 ctcagcctcg tcatacctgt tggcaaggga gtacatgctt atattcacag gcataaggtt
    22741 tatcggacat gtttcaacac atttgccgca tttaatgcag ttggaaggtt caggcagcct
    22801 tgcttcctct tcagaaagca ccaatatacc ggatgtgccc tttattgccg gtatatccag
    22861 tgaaaactgg gttataccca tcatcggccc ccctgcaata atctttccga ccttgccctt
    22921 aagccctccg cattgttcta ttatatccct gaaagatgtt cctatcttaa ccaatagatt
    22981 tttggggtta tttacccctt cccctgtaac agttactatc ctttctatta aaggcatacc
    23041 tgtctttata gcagttgcaa ctgccgccgc agttcctacg ttgttgacta caactccggc
    23101 atcagccggc agccccccgg agggaacctc ccggccggta catgcataaa taagctgctt
    23161 ttccgcccct tgaggatatt tggtcttaag tgcaactact tcaatatcct caattcctct
    23221 ggctgcttca aaaaccttct ctattgcatc aggcttgtta gtctctatac caatgaatcc
    23281 ttttttcact cccagggctt tcatcagtgc ctgcagccca aacaccacgt tctccggatg
    23341 ttcaagcatc agcctgtggt ctgaagtcag gtaaggctca cattcggcac cgtttaatat
    23401 tactgtatcc acatttttgt ccggcggcgg agcaagcttg acatgggttg ggaaagtagc
    23461 tcctcccata ccgactatac ctgcttcttt tattatgttc tttatatcct cagctgaaag
    23521 gactgaaata tctcccttgg gaaccacctg atccgaaacg gtgttctgca tgtcattctc
    23581 aatcactata cagggcacag ccgttcctcc cgaatacagc ctcggctcaa ctactgcaac
    23641 ttttcccgat acactggaat gcaccgggac tgatacaaat cctttagcct cccctatttt
    23701 ctgtccaacc ttcacctcat caccagcctg cacaataggt tcacaaggag cacctatatg
    23761 ctgctgcatg gggattacaa caaccttagg ttcattggct ctctctatcg gcaggctttc
    23821 agttgccttc ttactgtgtg gtgggtgggt tccgccctta aatgtcagtg ttcttatatt
    23881 caatttgctc accccttaat ttatataaat aagtaaatcc caacattatg accttataaa
    23941 ctcacaatta gtaactattc gacgcgtgta tacaaaatcc tttagttgta tttgttttta
    24001 ctagataatc ttttaacatt ttttgaatat gttcttccgc taatattatt caccaatctg
    24061 acaatactat ttccgaggtg aagaatgcta atgcacacag acatattata atatagcaaa
    24121 gctggttgaa aagtcaaagg aaatttgtcg tgaaaattcc agggaatatc tgattattga
    24181 tattgatgaa aaaggccgcc tcagcgtttt caactaagac agcctttttg aagctttttt
    24241 gttttttaac tcttctctat ttccttatta tcgccttgaa tgggcacttc tgcatacaaa
    24301 ctccgcattt tatgcacttg ctggaatcaa tggtgtaagg agtctttggc tggcctgaga
    24361 tacactgctg cggacaattt ttggcacata tcccgcagcc cttgcacttg tcggctataa
    24421 taaagtaatc cagcagttcc ttgcatactc ccgccggaca tttcttatcc ttaatgtgtg
    24481 cttcatactc atctctgaaa taccttatag ttgaaagtac cgggtttgga gctgtctgtc
    24541 ccaatccgca gagagccgca ttctttatat tctttgcaag actttcaagc ttatctaaat
    24601 cgccttcttc acctttgcct tcggttattc tttcgagaat ttccagcatt ctcttggtgc
    24661 cttcacggca cggcgtgcac tttccgcagg attcatcaac tatgaagtcc atgaagaacc
    24721 ttgctatgtc aaccatacaa ttgtcttcat ccattacaat cataccgccg gaacctataa
    24781 tagaacctaa tgcagcaagg ctttcataat caataggtga gtcaaggtgt tcttcaggaa
    24841 tgcagccgcc tgaagggccg cctgtctgaa cagctttgaa tttctttccg ttggggcagc
    24901 ctccgcctat ttcatatatt acttctctca atgacgttcc cataggtatt tcaacaagac
    24961 ctgtattgct tatcttgccg ccaagtgcaa atacctttgt gcccttggat ttttccgtcc
    25021 ccatgctggc aaaccattca ggccctttgt tgaatattac aggtatgttt gcataggttt
    25081 caacattgtt aagtattgta ggctttgcaa acaatccttt atttgcaggg aacggaggcc
    25141 ttggcctcgg catacctctc tgtccttcaa cagaagctat aagagctgtt tcttcgccgc
    25201 atacaaaggc tcctgctcca agcctcagtt caatatcaaa tttaaaacct gttcccatta
    25261 tattatctcc aagcaggccg tacagccttg cctggcctat tgctattccg agacgtttaa
    25321 cagctatcgg atattccgcc ctgcagtaaa tatacccttg gctggcacca atagcataac
    25381 cggcaatagt catggcttca agtattgagt gaggatctcc ttccaaaacg ctcctgtcca
    25441 tgaacgcacc cgggtctcct tcgtcagcat tacaaagaac atatttctga tcatttactg
    25501 attttgatgc aaattcccac ttcattccgg tggggaagcc ggcgccgcct cttcctctga
    25561 gcccggatct cttaactgta tctattactt ctgccggtgt cattttggta agtacctttt
    25621 caagagcctt gtaaccgtca aaagctatat attcatctat attctccgga ttaattatac
    25681 cgcagttcct taatgcaacc ctaacctgct tcttgaaaaa atcaacttct tcaagggctt
    25741 tcagccggtt ttcttcaact gactctgcat aaagcagcct cttcacaatt ctgcccttta
    25801 ccagatgctc ctttacaatt tcctctacat cttcaggctt aaccatactg tagaaagaag
    25861 cttccggata aataataact accgggccca gtgcacaaag cccgaaacag cctgttcttt
    25921 ccacaacaac ttctttttca agaccatgct tctttatttc ttcttcaaag ttctttgcta
    25981 tctgttcaga atttgaggaa gtacagccag tacctctgca aatcattaca tgagaacgat
    26041 acatctccat taatattacc tcctacagat tactatattc ctttctgtcc ctcaagcctt
    26101 cctttattta ctttcagcac cgattgtata tccctcaaca accttgccct tcataatatg
    26161 ttcattgact attctaatag cctttgacgg atttacctta acataagtaa ctttctcttc
    26221 gccgggttta tatacttcta caataggctc aagcctgcac atgcctatac agcctgtctg
    26281 ggcaactacc acatccgaca gatcatgttt ttttatttcg tccattattg ttagaagtac
    26341 cggccttgct cctgcagcaa taccgcaggt tgccataccg acaactattc tggtgcctga
    26401 tctatctttt ctgatattga tcgtatccag ggttttcttt ctgatttctt ccaattcctg
    26461 aattgttttc atcttacaca cctccgcgta tattctttaa accttcatta atataatcct
    26521 taatccacat cagaacctcg ggctccccca agttaatatc attacccaaa gcagtcctga
    26581 tctcccttgt atccaggcaa aaaacccttc cgtcaataat gtgcctgtat acaaaatcaa
    26641 catttctatt aacatcagat cctgcaagaa ttaaactcaa catggtttca gccatatctc
    26701 ccataggagc cctgtctata tggctatgtt tgaatacagc tttaagccgt gttcccacat
    26761 ttttttccga ttctacgaca agatcccctt cacatctact gcatgccgcc agcataagag
    26821 gaattcccaa tcctaccctt ctgcttgtcc ttgtagttac aaagggatcc attacctttt
    26881 ccagaaaatc cttatccatt cccttaccat tgtcatttat acagatagta agtcgatcct
    26941 cttttgtatc ttcttctatt gatatctcca caaggccggc tcccgctgaa atcgaattct
    27001 gggacaaatc aaggatatgc agtgataaat ccttcatcct gatattaata cttctttaca
    27061 atatccggga tatcatccgg aactaatttg ccgtatacat cctcaccaat catcattacc
    27121 ggagcaaggc cacagcagcc caggcatctg gttgcctcaa gagtaaactt tccatccgct
    27181 gtcgtacctc cggttttcac gttcagctct tcttcaagtc tttcaagtac tcctttggag
    27241 ttcttaacat agcaggctgt tccgagacat acgcttattg tgtgcttacc tctcggagtc
    27301 aaggtgaacc ttgaataaaa agtagctacg ccgaatattt cagctgccgg aatgtgtaat
    27361 acttccgcta tcctcttctg tacttcctca ggaatatatc cgaataattc ctgtgcttca
    27421 tgaagaattg gtattaaagc acccttctga ttcttatgct tctgtataaa gtcctccaat
    27481 ttctccatgt tttccttcat tgctgcgtca gacattaaag agttccctcc ttcaaatatt
    27541 tataattgga aatagtaaca gcttatattg aatgcacgga ttcaataaaa tttaggttgt
    27601 tgtttctcac gaaaaaattt attgatataa atttgtcaat tacgtcctac aatattgtat
    27661 cacatattag atattttttt aacaaaaata aaaaattatt tttaaaagta ttataatatt
    27721 tttcattttc ccgtttattt ttttctaaaa ccgtatataa taattggttt ggagtattct
    27781 ttatttaagg tttttattat ttggggtcca gcttgttgaa aagctcactg acggttaggc
    27841 aatccagctc aataaaaaaa tccctttcgc ttatatcttc aagataatga gcgtcagagg
    27901 atattatata attgaacctg ctaagaaaag gatacttctt aaaagctgca tccggagtgc
    27961 atctttttga tatttccact gttttgacct taaggtcagg aggaatgaaa ccaagattgg
    28021 taatgatact gaagccctgt ctgtccacat gagcgggtat acacacccct ccaagccctc
    28081 tggtcaatat gaacacatca ttgactgaga gtattgtgga agacagaagc agcttatgct
    28141 ctttccctat tatttcatct gaactgttaa aaatcagctg gcgtccaaaa atttcttcgt
    28201 tgttctctat atccggtaag gaattataaa caatctccgc gaatttcatt gctccttcca
    28261 ttctcttgaa aaggcacaat aaatgaactt cctcttttgt ctgaacctcc attcccggaa
    28321 taaccatcaa tccgttctcc tttgctgctt ccataaccgc aggaagattg ccgcaggaat
    28381 tgtgatctgt aaccgctatg atatcgagcc ctttcaacaa ggccatattt actatgttgc
    28441 atggtgtcat atcctcatca ccgcacggtg ataatgcgga gtgtatgtga aaatccaccg
    28501 caaattccat attactcaat tcccatcctg tatagttctt ttgcaatccc atatgcgctt
    28561 aaacccgact ggtacacagg tactccctca tcctccgctt ttcggagagt atcctcctca
    28621 acctccacgc cttcgggtat aatgatacag cccagttcaa gcaaagctgc cactgcgaca
    28681 atatttgcat gtgtctgcac ggttatccat gcgtcccctt taccggcatg ggccataacc
    28741 cagcttaaaa gatcacatat atacactccc gtaatctccc tgctggttcc ggcctctcct
    28801 gccaacagct tcaatcctat tttttctccg gcatctctta ctaacatgtt aaccctccct
    28861 atgattgcgg tttttcttta ttaaagcttt tcatggtttt gactatgcaa actgcattcc
    28921 ggttttcacc cttcactatg tcctctgcaa atgccttgca ggttggagcc ccgcagacac
    28981 cgcagtctat tcccggcaag tccttcagca tttcctttat cttttccatc ttcttaatgg
    29041 atttgcagat atcatcatcc agacgcatta cggctttggg ctcaatcgct tcagtcaatc
    29101 ggatgaatcc gctcttatac atttctattt gtccctttat ctcttcatca cttggatttg
    29161 aatccggaag cccttttgca agggttttca tgcgtctcct ggctataaaa gggttctcta
    29221 ttgtaagagg ccctcccaca caacctccga cacaggccat tccctcaaaa aactccaatc
    29281 cttcaagctt gtccatttca atttcttcca gaattttaat aacctcatgt attccgtcta
    29341 cagcaaggta gttttttatg cccaaaggct ttccctgtcc tcctattata ggccataaca
    29401 aagattcctt tgtacttctg gtcagatcct ctgtattgct gcctaaattt ctcagcatca
    29461 tgccatatac atccttcatt gacagtacac catcgatgta ggactttttt atgcctatgg
    29521 ggtgttttac acttgtcatc ctggctgtgc agggagaaat atagattacc cctatctcct
    29581 ccatggaaaa acctgtcctt ctgacagcct cagcctttgc aaggcgtgcc gctatctcaa
    29641 ccggtgtttc tatatccgca atattaggca gcagttcagg gaatctgatc tgtataagcc
    29701 tgagggcagc aggacaagaa gaatttatta tgggacgttt tttctcagga ttcttgagaa
    29761 tgtggttttt tattacatta gttattatct ctgtcccggc agatgcctca aatatttcat
    29821 caaaacctat cttccttaca gccgtaagca tttcattgat tccgacattg cctcggaatt
    29881 gcccgtacag caccagggac ggtatagcaa ttgtatattt atatttcttt aatatatctt
    29941 tactgtctgt aacagcattc ttggcatgat tgggacatat tcttatgcat tcaccgcagt
    30001 caatgcattt ttcacctatt atacaggctt tcccttcttt cagcctaata gcctctgtcg
    30061 gacatacctt caaacagctt gtacatccga cgcatctatc tgcatcaagc acaaccgaat
    30121 gaaagtactt ctctttcatt ttattcacct tgctcacctg ctttcaaatc tacttcaata
    30181 acaataagag tacctgttcc ttctgaagaa gttacatata atctgtctga gcatcttttc
    30241 atattaggca aacccatacc ggctccaaaa cccatttccc ttaccttatc ggtagccgtt
    30301 gaataacctt ccttcattgc cagttctacg tcagctatgc ccggccccct gtcatttgct
    30361 gttatcctga taatccccgg ggtaatctcc aacacaatct gtcccccggt agaatgtata
    30421 acaatattca tttccgcttc gtaagcggca atggaaatct ttcttataat acttgagtcg
    30481 atacccaatt ccctcaaaat tttctttact ttgcttgatg tttctcccgc tctgacaaaa
    30541 tcatcttttt taacatcaaa tacgtactta atatcttcca ttaaataatt cctccaaata
    30601 gaaagccaaa atccgatcaa tcaatcaatc aatcaatact tactccttcc agcccgttgc
    30661 tgtaaagctt gcccgaggct gtgtacatgg tatccctggt caacagcagc gcaatgtcct
    30721 tctcagtagc caactggact atatcttcac cgggtttttt ccccctgaca aaaactattg
    30781 cgcttagatc cgccatttcc gcagtcctta ccacctgctg gttggtcagt ccggtaagga
    30841 gcagggtttt gccctttaca aaagctagca cgtcgctcat aagatcagat ccgaaagcat
    30901 atgaaacctc gttctccagc agttcatctc cgcacagcaa ttctgcgttc aatacttctt
    30961 taacctcaca tattttcata tataaacctc cttcagagca ccatctatgg tggtatgctt
    31021 acaatatata tttaattata caatataaaa agcataaaat cgacaattaa ttaacaaagt
    31081 ctttccgttg cttcttttta ttgatatgcc ctttaatctg tactaaaata gaaccaacag
    31141 gaggtgattg gattgtcttc cattttatcc ggcattcttc gacaaaagct gttagaaata
    31201 caaaatcagc ttccacccta tgtcaagata ataaaggaca accctccaag ctttgaggac
    31261 gtacttaaca aggagatcaa tgagcaatcc gatgttcaaa ataattatgg cggagattac
    31321 agccatttaa ttaaagctgc tgcgaaaaaa tataatatca acgaaaatat aataaccgcc
    31381 gttatgaaag cagaatccgg ctttgacccg actgcagtat caaggacagg ggcgatgggc
    31441 ctgatgcagc ttatgcctga aaccgcagaa gccctgggag tacataacgc ctttgacccg
    31501 cgggaaaata tagaaggagg cgtcagatac cttaaggata tgctgacaga gtttggcggt
    31561 aatctggagc ttgctcttgc agcatacaat gcaggcccca atgccgttaa gaagtacggc
    31621 gggataccgc cttatgaaga gactcagaac tatgtgagaa aagtaatgtc aagccttaaa
    31681 aaataaaggg acgtgggggg acgtttcggg acacggggac gtttctcctg tccctttaca
    31741 gcacttccct tttcattatt ataaaatcct ctgcgagggc attgagcttg atatactgtt
    31801 cgatagtcct gaagtagctg aactcaagct tctgccaaaa acatttccca acactgttac
    31861 gggcaattat tcctatatag aacctcttta catcatagct cttattcagc atgtttatca
    31921 acgcttcagc agccttggtc ccaattccca gcctctgata cctgttatcc acaagaattg
    31981 aagatatcca tgcttcctcg ctgttttcat aatcaatcct tccttttatt acaccaacca
    32041 actgcaaggc atcttctttc tcaagaaaaa taccggtaaa gaattcatgg ctgttgacaa
    32101 gcacttccag atacttttcc tttatatccc tcagggacat tcttctgtct atgcctgttg
    32161 catatatgtt ctgatcattc tggttataca gattaaggac agccttcaga tcctccttgg
    32221 taatgtccct gaatatcaga ttatctaatt tattattaag agtaagcatt ttttcacccg
    32281 catatcatat gtttcgcagc aaacatcttc tgtcaaattc aaatatcgca aaacactttc
    32341 tttgatttat acatcttttt gattactgaa atatattcta caaaacataa taaattcctc
    32401 ttttaattat tactacagca ttgcaataag ctaattccag acgtttccat atgatttaaa
    32461 ctaattgctt tacattatcg tgatttaata tataatttaa ttaataggaa tattctacta
    32521 accaatttac ttgaggcagg tgatataatg gcagaaataa gaataatgag gacttctgat
    32581 tgtaagccgg ggatggtatt ggcttctgac atcataaacg actatggtgc agtgatactt
    32641 taccagaact caatattgga tgaatactcc ataagcaaat tgatgaagct gggtttgaat
    32701 tttgtcaaga tttacaagga ttatgaattc aaagaaaaga agcacgctgt cattgaggcc
    32761 caatacaaca acaatcttga ggaatttaaa gaaataatct gggatataag ctgcggaaaa
    32821 acattggaaa tggaacgggt aagcgaagtg tcaagaaaca tcagcagtaa ttttgactcg
    32881 atacacgatc ttgtctcatg cctcagtaag gtcagatcaa ttagtgaata tacctacgcc
    32941 cacagtctga atgtatcact tttatgctcc ctgctgggct cctggctgaa cttggggcat
    33001 aagcacattg agcagctttc ttattgcggg atattgcatg acatcgggaa atcaaaaata
    33061 cctcccgaaa tactcaataa acctgataca cttactgaag aagaatttga caaaataaag
    33121 cagcaccctc ttattggcta taagatgctg gaggacaata aagaaatcag taaggatgtt
    33181 gctttaggag tattgatgca tcatgaaagg gaggatggct ccggttatcc ctttggattt
    33241 aaatcggaaa gaatttatta ctatgcaaag atagtggccg ttgttgatac atttgatgcc
    33301 atgacctcaa accggattta taaaaaacgc cagacacctt ttgacgtact ggaaatgtat
    33361 gaaagtgaat atcttacaaa gtgtgatgcc ggtataatgc ttactttcct caaacatata
    33421 tcaagttatt atataggcgc aaaagtcagg ctgagcgaca gctccatcgg cgagatcgta
    33481 tatattaaca caaacaggat ttcccggcct ctcataaaat caaaggaaac aataattgat
    33541 ctcagcatgg ttccggaact gaagatagta gaaatgctct aagtatttct caagctgacg
    33601 ccataaaagc agcaggtttt gtggtatact atattaatgt ggaatacgat tagcaaaagg
    33661 aggaattatc atgccattgg taacatcaaa agaaatgttc aggaaagcat atgagagcca
    33721 atatgcagta ggggcgttca atgtgaacaa tatggaaata atacagggga ttgtcgatgc
    33781 cgccaaggaa gaaaacgcac cactgatact gcaggtttcc gcaggtgcaa gaaagtatgc
    33841 aaaacatgtt tatcttatga agctggttga agcggcggta gaagacaccg gcctcccaat
    33901 ctgcctgcac ctggatcatg gtgaagactt tgaaatatgc aagtcttgca tagacggagg
    33961 ctttacatcc gttatgattg acggctccaa gcatcccttt gaagagaata ttgcagtcac
    34021 aaaacgagtt gttgaatatg cccatgagag aggaatagcg gtagaggcag aactgggcaa
    34081 actggcaggg gttgaggata ctgtcaatgt aagcgccaag gatgctacat ataccgatcc
    34141 cgaccaggct gttgaatttg ttgagagaac aggagttgac tctcttgcaa tagcaatagg
    34201 caccagtcac ggagcatata agttcaaagg agaacctaag ctggattttg aaaggctcga
    34261 gaaaataact aagcttctgc cgggtttccc cttggttctt cacggtgctt ccacagtgct
    34321 tcccgagttc gtcgagctgt gcaacaaata tggaggcaac gtagcaggag cacagggtgt
    34381 accggaggat atgctgctga gggcaggaaa actaggcgta tgcaaaataa acatagatac
    34441 cgacctgaga cttgcgatga ccgcttcaat aagaaagcat tttgccgaga acccgggaga
    34501 ctttgacccg agacaatatc tgaagccggc aagagaagcc ataaagaaca tggttaagca
    34561 caagatgagg aatgtattga actgcagcgg caggatgtag caaaaaaaaa tgcggccatc
    34621 cagtatactt cattttctta catttttcaa gaaacgcggg tattgatgaa aagggatatt
    34681 gcaaactacc tgataagtag ttagcaatat cccactttat attacactca gcttctatta
    34741 aggatttagt tcctccaata tccactgcgt cacctgcaat accaggggcc cgcaaagttc
    34801 attgtgcctt ttcgcctttt cctcttctgt ttctgcggtt tttttcatat ccaagcctgt
    34861 aatatcataa caaaaggttg atccgtactt ctccttgaat ttcccgaata tcctgtctgc
    34921 cggtaagaat gatgtatcct tgctgtcttc actggatctt ctgccgccga ccaggcccat
    34981 gcagataaaa gcagcattca acgctccgca gttgcttcca tttttgctca ttcctgcccc
    35041 gaaaggagtt gctatttttt ggggtatttc cttgtccgca cccaaataat cacatacagc
    35101 cattacaacc gattcgcagc agttatagcc gccgttgaag taaaccatta ccttttccag
    35161 agcctcttca cgtttatcca ttatacccct cctattttat tgctttactt ttatttatcg
    35221 gttatcagat ttctaatatg attatatcat accgccggca ttctcgtcat atccggagaa
    35281 aagccttatt ataccatagc tgcagcaata aggctcttaa taatatatgg ttgttctgat
    35341 gatattgatt ttcttatcgc tgcagctctt atctgtcata aggttgtaat ccgcggcttt
    35401 tcctttgcat atggttgtcc aatccccgct gaagctgtct ttttcaaagg cttttaaaac
    35461 agtacggtga tggccttgat gttcaatttc aacttctcct tcaatgatca tcaatatcct
    35521 tgagattcca ggcaagggag taaatgttga ttcatcagcc gcaacttttg cggaactcaa
    35581 tctccacctg aaattacgat ctgcatatac tgcattccca ggatatatat acaattgtgt
    35641 cgtcgtgcct cccgaccatg tactgacttt gccctcgctt tttcttatta ttcttatgct
    35701 gctgttcata tcccaccatt tatatccttt caaagtccaa cctttagcaa taatgcatct
    35761 tacatcagta tttgtgtgct atctcctgaa taggctcagc tttgatttat aataaaagag
    35821 aagtctggaa aacacataat catataccag gaataccgct tgcatcgctg tgataaaaac
    35881 tgccgtcagt atgcttcctc ccgtgaactt agccggaagc gccgttatga acaggttctt
    35941 gaaaaataaa taccatagga agattgacat gttaaaaaca gtaagcttga gcagcaattc
    36001 aactgcagcc tttcttccct tttctataaa gtacttgaaa acgggataat ggccgaaaaa
    36061 aaatacaaag ggtactgcac ctgtaatatt acctgtaatc agtatattta gaagggaagc
    36121 agcgaaataa acagcaatcc ccgctcccgc tcccaattct gcaacagtaa ttgccaccgg
    36181 caatgacgcc aaaaaatata acgtcagttt acccgtaggc attatagtgc ttaaatacag
    36241 gaacaccact gctatacctg caaatatgcc tttaaaggcc atttttctgc tcataacgcc
    36301 actcccccgc gttacccatt cttaataatt tcgaaccccg cccaaatctc gcatctagtg
    36361 actcatatct cttgtatgcg catgccagtg cgcatattct aacagcatct gatgaagtcg
    36421 ccgcccatgc attcacagca gcaatcggta catatcatgc actgacacaa agtgcataga
    36481 tccggtccgg aagtatagcc ccttctttca tatacagtag actgatactg tctgttggca
    36541 ttcatgatac tgttcaatgc atccctgaat tcataattgg agggttccat atccacggca
    36601 gtcttcaagc atgtgacagc ctcattatac caaccccggc gcatgaaaat aaggcctctg
    36661 aggtaatacc attctccacc tctgaagctt attctattca atatttcctc cgcagctgca
    36721 gtattgccca tattcacata tatccttgcc tgatcataca agcctctgtc cccattctcc
    36781 cgattgttcc cgtaccttgg ctcctcctgc tgtctgtagc tgctgttcct ttcctcggat
    36841 tccgctttct tcataagata atcgtaagcc tcatttatct ctttaagctt ttcctcagca
    36901 agatcagaga ggggattgtt ctgatactgg tcgggatggt atttcctgac caattcctta
    36961 tatgcccttt ttattccttc aacacttgta ccttctctaa tacccaatac ctcgtatgga
    37021 ttcctcatct ctcacacagc tccttttttc ccctgcaatg caattcaata ctctttctgt
    37081 ttttccgtat aaaccctcat acgcaatgtt gcttattatg cctttattat ccagcttcaa
    37141 cagctccaaa gaacccgtgg cctgtgacaa tgcctgaaga aggccgacct tgacttcttc
    37201 agcaatggaa gctttaaaat cttttatatc ctgattttca tatttgtatt gtctgagaag
    37261 agggttatag cttccgctct tgatatctct ttccatatca tcaaaggcat caataatata
    37321 aatccatttc ccaagattgt agcctatcca tcccagtatc ctcggcactg atttgtcatc
    37381 accgctgtat cctgacgcaa gcagctgcat catcatattc gcaaagggtt ctgccgcttc
    37441 atccgtcgaa ctgcatccct tcttctccaa ggatatctgt gaagaaacag atgaagctat
    37501 gattctatca atgtccttat ttgtcaaagc agctctttta tagcccttat gaaaaaccag
    37561 ttttaaaaac gtcgggagcc atcctccttc atcccttata tcatccttca atttataata
    37621 cgtaagcagc acattaatgt ctgccgcgaa atcgataccc ggactttctt ttactatcca
    37681 ttttcttttc accggattgg caatacagct ttctttctta agcaccggtt tttcgccgtg
    37741 aacagaagcc agcagcaatc caagaaaaac gctgtcataa ttcagtgcaa atcttgagag
    37801 cactccaaag cttctgccca ttgatttgca caagccgcag taatagcccc tgaatagttc
    37861 gtattccttt attttaagct cctgtaccga aggctttata tatccaaaca tatatactcc
    37921 tatagcaaga cttgaatatc tttaaggcat tgtactttaa tattatatca taaggcaaga
    37981 gtcattagaa agaggacatg gggacgtttc gggacatggg gacgtttcgc gtgtcccact
    38041 ggaagtggga caggggaaac gtccccatgt ccccgtcccc gtgtccccgt gtcctcctcc
    38101 aaataaaagg gggatatcgt taactgcatg ttaaacagtt atgcgatgtc cccatctttg
    38161 tatctgaatt tgattgtttt ccaagttaat tcatatcagg tagctcttga ggaattccgg
    38221 tatcccttct atagccctgc tgcagctaat cgtaaaatcc acgccgaatt tttcatgaag
    38281 tatttcaaga ttctttatga agccttcaag gcaatcatcc tgcgcattgc ttacgatctt
    38341 ctgcccgtca atgtatatgt tctctatgtc aaaatcctgt gatattatcc cgcagataaa
    38401 accatagaat gatttaagat tctcaagctg gaaatctccg gtttcaatca gtcttattct
    38461 ccggttaagg tcatgaattc tgtttctgtc cctatcgata tatacaacat taccctttgc
    38521 tgttctgcct gattcattag ccagttcaat cattcttttt gttttgcctg agccttttac
    38581 gcccagaata aacttaacca taataattac cccctattat tttttattgt ggagttacta
    38641 taaagatact ataacttaaa gtatttttca atctcaatat cgccgaaaac ttctaaatat
    38701 tctgtttttt aaaagtacga cacatcaaat tccgctgttt atgtatttta tcaatttcct
    38761 taaaaaaatt tttgtaaata tactgtatat aattactatt ttatacagat ttattcataa
    38821 aatacgtttg cccatttagg catggaatga attatcactg atgcagcggt atattttggt
    38881 atatttatgc gttccagcgg ctctaatggt taaatttgcc gaacttttag aaaatttatg
    38941 tatttacaca atcccttttt tttgagataa tgttaaatat acaaataagg cttttttgag
    39001 ccttttataa tgttcctggg gtttcaactg catagggtag gtttaaacta gcaaaacgaa
    39061 atttttctaa atttatttta agtttaatat atctgtttaa acacgcctca aagcagtgaa
    39121 ataaaaaaaa tttaggaagg ggtaatgtta atgtctacaa agacaaagag agaagcgaca
    39181 ttgtttgaag cattaatacc ggttgttatt acaataggat tccttatgta tgcagtgctg
    39241 cctcaatttg gcgtaggcca ggatgtgcat gttccgttaa tcctcggcgc acttatagct
    39301 tctattgttg ctatcacgcg acttggatac acttggaaag aagttgaaga tggtatcgta
    39361 tcgacaatat ccgatacaat gcaggctatc ctaattcttg ctatcatagg tatgataatc
    39421 ggtacctgga tacttggagg tattgtgcct actcttattt attatggatt gcagatactt
    39481 tctcctagct ttttccttat tgcagcatgt ttgctctgct gtgtggtatc ccttgcaaca
    39541 ggaagctcat ggacaactgc aggtacagtc ggtattgctc ttatgggagt tgctgcaggt
    39601 ctcggtgtta atccgggtgc agcagcaggt gctattatat ccggtgctta tttcggagac
    39661 aaaatgtctc ctctgtctga tacgacaaat cttgcacctg caatggcggg agcaaacctt
    39721 ttcgaccatg taagacatat ggtttatact actggcgtca gcctgataat agctcttata
    39781 ggttttggca ttcttggtgc cggctatgct ggcaaagagc ttgatatatc agccattaat
    39841 gaaatacttg cccttatgag cgataacttc gttatcaatc cgttacttct cctggttcct
    39901 ctcattgtta ttgtaatggt tgccagaaag gttcctgcca tacccggtct cttcataggt
    39961 acacttctgg gtggattggc agcaatgata ttccaggggg caagctttgc aggtgttatg
    40021 gatgctatgc agtatggcgt agtatcggaa acaggaaatg caataataga cgagcttctt
    40081 acaagaggcg gatatcaaag tatgatgtgg actatctcac ttatactggt tgcacttact
    40141 ttcggcggtg tacttgagaa aacaggcatg ctggagacaa taggcaaggt aatattggga
    40201 tatgcaaaga gcacaggggc actggttacg gcaactataa tcacaagtat cttcacaaac
    40261 gtacttgcag gtgaccaata tttggctttg gtgcttccgg gcaaaatgta caaggatgaa
    40321 taccgcaaga gaggccttgc tccaagaaac ctttcaagat gccttgagga cgcagggaca
    40381 cttacttcac ctctcgttcc ttggaatacc tgcggtgcaa caatgtcaac atatcttgga
    40441 gtaccaacaa ttcagtatat accatactgc ttcctgaatc ttgttaaccc gatagtctcc
    40501 attgtattcg gtttcaccgg aatcacaatg atgaagctgg aagatgaccc gtcggctccg
    40561 gagtataaag ggccaaaatc aaaaaatgaa aacgtatcag cttagtaaat aataaaagcc
    40621 tgttgcggat gcaacaggct ttattcatat ctcaatcaat tccttcgtga acttgttcag
    40681 cacttctact ccattctcag ttaccattac aaggtcttct attctgaata ccataccggg
    40741 ctgcagtaca gtacagctta tatctgatat atacggttcc tcatgaacct ctttgtattc
    40801 ctttgaaacc gggtttcatg tttcattgta attattcatg cgtttccccc taaaatcaac
    40861 tttaatcaac gcaattatat cacattcttt tcccactcac atattatgat taaaaaaggc
    40921 gtctcacacc ggaccttttc ttgcatcccc attatatagg aaagtatact ttcccataca
    40981 ataatatgcg tatttcggga ggctaaaaaa catgaacctt ggtattgacg gaagagtaat
    41041 aacctggcat atcggctccg gcttggccac ctataccaat aacctgctgg aaaacctgaa
    41101 taagcttaaa cagttaaaga atatattttt gttttaccca tctaagaatg gaactgatta
    41161 ttttgaaatc aatgatatac caaaaaacct tctgattggt gaaaggcgcc ttgatttttg
    41221 gcgaaatgta tacgaactta aatgggatgt caattatccc attgatgttt tccacaatac
    41281 cgtcaatggt atagggcttc ccaattgctt caagggcgta aaagtaataa ctctccacga
    41341 ccttattcct tatattatgc ctgaaaccgt agacaggccg cacctggact acacattgaa
    41401 gcatacccct tacattttgg atgatatatc acacataatt actgtgtctc gccactccaa
    41461 atcggatatt caaaaatact tcggaattga agatgacaaa ataacggtaa ctcatttggc
    41521 ggcagaccct atttttaggc ccttggatag ggacaatgca aaaaaagtta ttttcaacaa
    41581 gtatggtgtg gataagaagt atgttctcta tctgggaggc ttcagccaaa ggaagaatat
    41641 tgccagattg ataagggcct ttaagaaagt aataattgag aaagaggagg ttatcaacct
    41701 tcttatactc ggagagcatt ccagatcctt caaagctctt tggaagctta ccgaagaact
    41761 ggagctgtgc gattatgtaa aattcctgaa ttttgtcccc actgcagatc tgccttactt
    41821 ttacaacgga gcagaattat ttgcttaccc ctctctgtac gaaggctttg gcctgcctcc
    41881 actggaagca atgcaatgcg gtatacctgt cataacctca aatgtgtctt caatacctga
    41941 aattgtcgga gatgcatgcc ttcttacaga tccatattct gcggatgata tatccgactc
    42001 aatgctcaca ctgcttaccg atggggatga atggcataga tacagcctga tgggcattga
    42061 gaaggctaaa gaatactcat ggtttaaaac tgctgcagag acccttgaag tatatgaaaa
    42121 atccatgaaa ggtatatgaa ttaaaacccc tgcggtctat accacagggg tttagtttac
    42181 tattaataat taaaggctgc tatttcgaag tatgctcttg ggtgcttgca ggttgggcaa
    42241 atttcgggag cttcggctcc tgtgtgaata tatccgcagt tcctgcatct ccaagttgtc
    42301 tgctcttcac gtttgaatac tttttcctct tcaaggtttt tcaacagagc caggtatctt
    42361 tcttcatgct ctttttcaac aagggctacc ttgccaaaaa actccgcaat atctttaaag
    42421 ccttcttcta tggcagtctt ctcaaattcc ttgtacatgg agctccattc ataattttcc
    42481 ccttctgccg catgcttaag gttctctgcc gtgtttccta ttccgcacaa aaatttgaat
    42541 atcaattttg catgttcttt ttcattatct gcggtttctt gaaaaattcc ggatatttgc
    42601 tcatatcctt ccttttttgc agcacttgcg aaatatgtat acttgttcct cgcctgtgac
    42661 tcacctgcaa aagcttccca tagatttttc tcagttttgc ttcctttcaa atccattact
    42721 gataaccccc ttataatatt atattattat ttattaataa taattatata atatctcaat
    42781 aaccaatata tgtcaagccg ttttcttgct cagaagcagc ttccccatgc ctccgtactt
    42841 ctctttaagg ctttgtatat agcactcaag atcattgctt tcataatatc ccttaagaac
    42901 attattcaaa ccgttgagag cattctttac atcattttcc tttagttctt tcctgcaaat
    42961 ggcatcatta aactcgtcta catccaccac atagtacctg ccgtcaccct ttacaataaa
    43021 atctatgtac aaatccttcg ccttccaaaa ttggccctct ttggatacgc ttaccatatc
    43081 aacgtagtat ttgtacactt ccgatgggtt gaattgaagc ttggcggatt gctggagctt
    43141 gtatatgcct atattctccc tggggaacag attgtattcc acccattcat gatatttatg
    43201 atcaacatat tttctgaaat aataaagata attctcatat accttcaatt cttgaatttc
    43261 ataattatgt atccttccgt cttttctgac gctttcaatc attgcctcaa aatccaggat
    43321 tgtatacttc aaaaaaaatc ccccctacaa gcctgttaat atctttttat taagtataaa
    43381 taatttacac tgtaaaggtc aacaaatatt attttaatat acataaaatt gcgctttata
    43441 acaaataata tcaatatccg attcatatat acccaagtta attgaactat ctctatataa
    43501 aaaatacaag ccgcgacaga taattctata gactatgtta atgatttatg ttaatataat
    43561 agtaaataaa caaccgaatc ctgggatgtt cgatatgctt gtatttgaac ctacagagac
    43621 gacacgggaa tccaaggtac atgacctgat atcaggcctc atttgaagtc tataatctgc
    43681 agaggaagat agaaagccat gtccggatac ccacctagcg agagctaggt gtcaaattac
    43741 agctaaacga cattttgggg caaacaatgc aaaaaagcta tggtaatttt ccatagcttt
    43801 tttgcattgt tatctttatt aattcttatt tctgccatac agttccgaca ggcttgcaag
    43861 cttctcctcc agttctgttg tcaacatacc ctttctaagt ctcaagcccc agttcctgcc
    43921 tcctattgtg tttggaatat tcatccgtgc ctcacttccc aagcacagca aatcctgcat
    43981 cggaattatt gccgtatttg cagggctgct caaagctgcc tcgataagag tccagcacat
    44041 tgctttctct ccgatttctc ccgatatatg aaaataatcc ttcatctcat aatctgcttt
    44101 ggggtttgct atcttcatct tcaggtacca gcctgttgtt gtgtcattgt catgggttcc
    44161 ggtatataca accgaattct cttcaaaatt atggggaagc aattcttctt ccaaagggcc
    44221 gttgaagata aactgcaata tcttcatacc gggaaaacca aactcatttt tcagttcttc
    44281 cacttccttg gtaataaaac ccagatcttc agcaataata tgcatatctt tcctatgtgc
    44341 tcttaaggtt tggaaaagct tcctgccggg agcttttacc cagcatccct tttgtgccgt
    44401 tttttctcct gccggtatct cccagtagct ttcgaagcct ctgaaatggt ctatcctgat
    44461 tatatccgcc atttgaaaca acagatccag cctgtctctc caccatttga atccgtcttt
    44521 ctccatctca ttccatctgt aatgggggtt tccccaaaat tgccccgttt cgctgaaata
    44581 atccggaggc actccggctg tctttaaagg gttgccctcc tcatccaact ggaacagata
    44641 actctgagac catgcatcac tgctgtcata ggatatgaaa agaggcaaat ccccaattat
    44701 tctgataccc tgtttgttta catattcttt tagcagcctc cactggctga agaaaacata
    44761 ttgcagaaaa ctttgatact caatttcccg ggacagctta tccctgtaat atatcattgc
    44821 tttggagtcc ctgaaggcta tttccttatc ccagagattc caggcagctc ctccgaaata
    44881 ttccttcaaa gccataaaaa aagaaaactc attaagccag taatcgttct cccttaagaa
    44941 cctgtcataa tcttcaccgg cgccggcagc ttcaaatctc ttaaaggcct ttctgaacaa
    45001 gttagattta aatgccctga ccttttcgaa ctccacaagc ccctcatcaa aatcaggcag
    45061 tggcccgata tcctcctgaa gcaaaagctt atcctcaata agcctctcaa tgcttatcag
    45121 catagtattg cctgcaaagg cagaaaagca ctgatacgga gattcaccat agcctatggg
    45181 gcccaggggc agtatttgcc aaagcttctg cctgcttctg gccaaaaaat ccgcgaaacc
    45241 gtatgcttcg ctgcccagtt ctccaatacc gaatttcgaa ggcagcgagg taggatgcag
    45301 aagtattccg ctttctctgc cgaacttcat tcaattacct cctcagctga tttggctcca
    45361 atcaatatac ttattaagct tatctgcaaa aagctgaagg tatttggcat ccagttcgtc
    45421 aaacctgttt tttatggggc tgtcaatatc caatactccg aatactgttc catctttgat
    45481 taaaggaacc actatttcag agtttgaagc actgtcgcag gcgatatgcc ccggaaacaa
    45541 atgcacatcc ggcacaagct gtgtcctcat ttctgccgct gccgtaccgc ataccccttt
    45601 acccggcttt attcttgtac aggctgtttt cccctgaaaa ggccccaata caagctcttc
    45661 cttcttcata agataaaaac ccgcccaatt gatatcctcc aggttcgtat aaagcaatgc
    45721 cgcggcattt gataaattgg caagccagtc agattcactt gagagcaaac cctccagcct
    45781 ggtaatcata atcttgtaaa atttatctct gtccgcagat actatcctgc tgttgtcctg
    45841 cattattccg gcctcctttt gtactcgttt cccttgtcat gcgtctatta tatagtaata
    45901 ttttcatatt gtaaattgct catgctcata ctactattat t
//
LOCUS       DAOOAB010000018        45720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1009_length_45720_cov_15.054199, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000018 DAOOAB010000000
VERSION     DAOOAB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 45720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45720
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_45720_cov_15.054199"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="PKU88_05740"
     CDS             complement(<1..135)
                     /locus_tag="PKU88_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPX68822.1"
                     /translation="MEKRRNFTPEEKAKIVIEVLREEKTLNEIAAQYEVHPNQLSRWK
                     A"
     gene            1062..2345
                     /gene="kynU"
                     /locus_tag="PKU88_05745"
     CDS             1062..2345
                     /gene="kynU"
                     /locus_tag="PKU88_05745"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HPX68823.1"
                     /translation="MKHEFRLGLDCAKELDMQDPLKEVRNRFYVRKDEIYMDGNSLGL
                     CSKDAEKAVLKVMDVWKEHGIGLWNVEDSKYFLYPSYLGGRMASLINAEANEVTVTNS
                     VTINIHQGIATFYKPTKDKYKILVDDLNFPTDRYAVDSQVKLHGYDPADVVKVVQSPD
                     GRIISEDAVIEAMTDDVALILLPSLLYRSAQLLDMERITKEAHKRGIYIGWDLCHSIG
                     VIPHDFKKINPDFAVWCNYKYMSGGPGTSAGLYINKKHFAMDPGLTGWFGNTKSTQFQ
                     LKHQYEHSNDADGWLTGTPPILSMAAIEGVLDIYDEIGIVRIREKSLKITDYLMYLID
                     ERLTKYGYSIGNPREDDKRGGHVALEHQEAYRICQALKRHKVIPDFREPDVIRLAPVA
                     LYVSYEDVHRLVDILEELTISKEYEDFEHTRSLVV"
     gene            complement(2494..2703)
                     /locus_tag="PKU88_05750"
                     /pseudo
     CDS             complement(2494..2703)
                     /locus_tag="PKU88_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2895..3392
                     /locus_tag="PKU88_05755"
     CDS             2895..3392
                     /locus_tag="PKU88_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68824.1"
                     /translation="MQKTYRFMKDTNNIRRIKYRSENNSFFDEKVSYIIDSLDDAHKL
                     SQRSFLQRVFEATFKLIPEAEKGSFYELNDDKYTPIFSMNYDFELLKKLSFNKDDAFI
                     DFECDDTSSIDAYQVYISKRDDSKFSKEIKNYSQQSRSVYLLRQNRLCCIRISVIPIT
                     RRLSN"
     gene            3563..3967
                     /locus_tag="PKU88_05760"
     CDS             3563..3967
                     /locus_tag="PKU88_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68825.1"
                     /translation="MDHIFKTIEKGVDTAGVMKRTPVPETSFTDEITNARLAQIASVN
                     ALAMTAIAPYPWIPVHEADYISLVSGANSICAETGVNPRDTEADTSKGRGLDVTACRK
                     MLWQAGFKHLAKGDGTRIELTPEYITKCETTC"
     gene            complement(4168..5262)
                     /locus_tag="PKU88_05765"
     CDS             complement(4168..5262)
                     /locus_tag="PKU88_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPX68826.1"
                     /translation="MQLEKQLEKIVGLMSDINADIHMIGPSSGLEYLTGINPHPDERF
                     KALFILKDKRHFYISPEIYYEETRELLGEKEDIFVWSDTDGFLSAIEKADKKYGLAGK
                     TIAVNDGIVAINMLDIGNFLDVKFINGNSILEDVRIRKTQEEKDCMRKAAKIADEVVE
                     ETIKFIKPGLTERDIAKKLEELYTEKGASMSFHPIVASGPNTSKPHYNGDSRVIQEQD
                     IIIIDTGCRYKGYCSDISRTIFVGEPTEEQRKIFDICVKATTAGEMAVREGITAEEVD
                     MAARNIIKAAGYGEYFLNRTGHGIGIAVHEGPYIRTGNKQILENGMCFSVEPGIYISG
                     NFGMRVENIVLVENGKAEILNKVTKDIIIV"
     gene            5578..6126
                     /locus_tag="PKU88_05770"
     CDS             5578..6126
                     /locus_tag="PKU88_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPX68827.1"
                     /translation="MVHRFESHTPSLDTKSFVAEGVQLIGRVVMKEYSSIWFNSVARG
                     DVNAIEIGCYSNIQDNSVIHGTDDCASIIGDYVTVGHGCIIHGATIEEHCLLGMNSVI
                     LAGSVIGKGSIVAAGSVILENEIIPPHSLVVGVPGKVIKQIPGSWEQIHAQAIKYKTL
                     WTERYGLLPNAGGERYNGEKIV"
     gene            6229..6858
                     /locus_tag="PKU88_05775"
     CDS             6229..6858
                     /locus_tag="PKU88_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HPX68828.1"
                     /translation="MAEQKASSKKVTLWTRQDIRSLEDLKKEGVLRIKRKHLVEKFDL
                     IADYIINLYSWFVNAADRMVPKPEGVEFPIWCSISEEYMLRPTDTEIVYVLEVDESEI
                     IYFDGCKWDYVLNHHYVPKDDKDLEEYERDISDKGFRDSYSFLDENTSHFYPEERKRV
                     MDSWIRVFETDQWDPFRIQANMWEIRPEMIKGILKKDVTIDAMLSQMIK"
     gene            6906..7790
                     /locus_tag="PKU88_05780"
     CDS             6906..7790
                     /locus_tag="PKU88_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX68829.1"
                     /translation="MSTAPLSKERSRAMLLLAATAAMWSIGGLLIKIVDASPLSIAGA
                     RSAITSIVLLIYLRRPKFNWSLPQVGAAISSSATAILFITATKTTTAANAILLQSTAP
                     IYVAIFGFWLLKEKTTKYDWLTVAVVMGGMGLFFVENLSTEGLLGNFCALLSGISYAF
                     FVIFMRMQKDGSPLESALLANIITALIGLPFLCRSVPSTSGWLFLLILGVFQLGIPHI
                     LYSSAIKKATALEAVLISIVEPILNPVLVFLVLGEAPGIYALLGGAIVLVSITLKCVL
                     TIIPLKIVSEDKKEQPLE"
     gene            8110..8463
                     /locus_tag="PKU88_05785"
     CDS             8110..8463
                     /locus_tag="PKU88_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68830.1"
                     /translation="MLPDDAELLEGKHFIGIGSYKPNMREYSESIYKLLEKIYIDVDF
                     AREESGDLATPCEKGWFKEENIRTLYSVVKADSIDRSRTTFCKSVGMALFDIFVAGNI
                     YNKAIEQGIGQKLNP"
     gene            8501..9526
                     /locus_tag="PKU88_05790"
     CDS             8501..9526
                     /locus_tag="PKU88_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HPX68831.1"
                     /translation="MMNFSHYITCIDAHTAGEPLRIITAGFPPIPGNTILEKRDYVNK
                     NYDGLRRMLMLEPRGHSGMYGCILVPAVTPDGDFGVLFTHNEGLSSMCGHGIIGVTKV
                     VIETGMIGCTEGENVIRIDSPAGRVTAYADVEKGKVLRVRFHNVPCFVYKENITVHVD
                     SLNRDVVCDIAYCGAFYVYIDADRVDLEVEPQYAEQLVKIGMEIKHKVMEEYTFKHPT
                     EPGIDWLYGTILMSPVERIGNRLISNNICIFAEGQIDRSPTGTGTGGRTALLVHKNIM
                     QEDDTLVNKSVIDTPMESKVISRCKVGQYDAVITEVSGTAHITGFNNIVLDPDDPLPE
                     GFRIIGS"
     gene            9611..10450
                     /locus_tag="PKU88_05795"
     CDS             9611..10450
                     /locus_tag="PKU88_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPX68832.1"
                     /translation="MERKVEQQVRGFRTQDGAGVNLVRVLGKGTASEYDPILMLDSFD
                     STNPDDYTAGFPMHPHRGIETISYVYRGYMTHKDSLGNEDTIGDGEVQWMTAGSGILH
                     EEKLPASERLLGVQLWLNLPAKDKMVPPAYRSIKNAEIEEVKLENGKLRLLAGEYEGR
                     KGYMSKYLPLDYYDLHLNADTSVSVKTERSRSVMIFTLLGDAYIGGESVKEKTAVKLT
                     SGEHVEIIATNKNAQLLFVSSTILAEPIAWGGPIVMNTKEELNKAFDDLKNGTFLQKK
                     ISY"
     gene            complement(10520..10765)
                     /locus_tag="PKU88_05800"
     CDS             complement(10520..10765)
                     /locus_tag="PKU88_05800"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPX68833.1"
                     /translation="MNKEYQVLFYTHTGAIKFERAMKKENISCELMPVPRSLSSNCGV
                     SARIYYNGQVDQLINDQVEKIYQKYDGNYILTYEAEK"
     gene            complement(10762..10968)
                     /locus_tag="PKU88_05805"
     CDS             complement(10762..10968)
                     /locus_tag="PKU88_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPX68834.1"
                     /translation="MKTIDTCGMSCPQPVLMTKKALDEEKDGIDVIVDNNTAKGNVQR
                     YLKSVGYSVEIEENDGTFVLKARK"
     gene            complement(10981..12045)
                     /gene="yedE"
                     /locus_tag="PKU88_05810"
     CDS             complement(10981..12045)
                     /gene="yedE"
                     /locus_tag="PKU88_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HPX68835.1"
                     /translation="MKEKTGIIITGGVVGLFAVLLVRFGNPANMGFCIACFLRDIAGA
                     LGLHRAEVVQYIRPEIIGLVLGSFILASMNKEFNSQGGSSPVTRFVLSIIVMIGALIF
                     LGCPLRMVLRIAGGDFNAIIGLIGFVSGIYVGIFFLNKGFNLKRSYKLTNLEGYLFPA
                     INVGFLALLIVAPAFVFFSKSGPGAATAPIWISLAAGLIVGALAQKYRLCTVGGTRDL
                     ILFRDNYLMLGFLSILAVALVGNLILGKFNPGFAEQPIAHTDGLWNFLGMALVGWGSV
                     LLGGCPLRQLILAGEGNTDSAISALGLIVGAAICHNFGLASSAKGVTLNGQISVGICF
                     LILLAISYANSEFLSKNAQS"
     gene            12236..13381
                     /locus_tag="PKU88_05815"
     CDS             12236..13381
                     /locus_tag="PKU88_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPX68836.1"
                     /translation="MKEIYMDNGATSFPKAPGVAESVYKYLIHVGANINRGSYSNSFQ
                     AENVVFETRELLCSLFGFNKPENVVFTKNITESLNVLVKGLFKSGDHVIVSSMEHNAV
                     MRPLRALEGKGVTFSRVLCKNDGSIDGKDLLPLILPNTKGVVMTHASNVCGTILNLEE
                     VGSICKEKGLRFIVDTAQTAGAIKVDFDDMCADAVAFTGHKGLLGPQGIGGFLVREGL
                     ADELSAFIEGGTGSLSDMEIQPSYMPDKFEAGTPNVPGIYGLNASLKYIQETGIQDIY
                     EKEMRLMGVFLERLMNMPHIDIIGKKDTKGRTGIVSVDFKTQDNAEMAHRLSSESGIM
                     TRSGLHCAPSAHKTLGTFPRGTVRFGLSHFMEEADILYAADQIYKLK"
     gene            13397..14287
                     /locus_tag="PKU88_05820"
     CDS             13397..14287
                     /locus_tag="PKU88_05820"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HPX68837.1"
                     /translation="MEFNQIKIFVAVARKKSFSKAADMLFISQPAVTSNVQKLESELG
                     VTLINRKTKNISLTEGGKLFYRYAVELINIYEKAEYALSGYKKNIEGTLELYASTIPG
                     QYLLPYIVKEFKKAYPLVLFSICHKDSSEVMEEILSGRINLGLVGAKYSSDALEYIDF
                     YDDRMVLIASPQKKFDSDSVSIRSLIGENILLREEGSGTRLLLENALKEKELDMSMFK
                     SQIINDSLEAIKKMVELDVGISLVSDIAVKREVESGQLKQYEISDLELKRSFSLVYCN
                     NRCLSPIEEKFKDFISNWKR"
     gene            14453..15070
                     /locus_tag="PKU88_05825"
     CDS             14453..15070
                     /locus_tag="PKU88_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX68838.1"
                     /translation="MAISERREREIEEMKELILSTAAMIANSEGYDKLSIRKIANKIE
                     YSPSIIYHYFADKDEIISHLMQRGYKKIISAIASANMISDSPEEQLKKMARYYIDAAL
                     KMPEEYMAAQLSKSPKTIKQTSFLYEGASDEKSVIGALCKCIKEINKDIDLEDSKCEL
                     ISQLIIASAMGLVIKLIAEKETGEEQRNRLVECFANEVVPKIARV"
     gene            complement(15357..16265)
                     /locus_tag="PKU88_05830"
     CDS             complement(15357..16265)
                     /locus_tag="PKU88_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPX68839.1"
                     /translation="MTLEQLRYFVETAHTLHYTKAAEQLNISQPSLSYAISQLCKRLG
                     VPLFKKEGIKVSLTEYGEAFLPYAESALNILIQGEAQLNRMNSPYGGNINLGYIYSVS
                     FDEIPHLIDDFYACQGNRNISFSFQVNMTNLLIKKLIEGTLDVVMAPSTEIVNECIEF
                     IPLFEQELFLVVYKDHPLASHTSVTIDDFKNEKFIMIKKETDLHFQTDSLFKKFNIVP
                     NIFFEVDECNSMASFVGAQLGIAIMPKIPSLDGYKVTAIPFEGRTVRRTISLLWNNRI
                     KHTPALKSFLDYCISSRSTGIKNTEK"
     gene            16731..>17464
                     /locus_tag="PKU88_05835"
     CDS             16731..>17464
                     /locus_tag="PKU88_05835"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPX68840.1"
                     /translation="MNLPSKITICEVGLRDGLQNEKKILQTQEKLELANGIIAAGFKV
                     IELGSFMNPKAVPQMADTDELFSTLNQIDGIELRALIANKKGVERAINCGCRKVKLNV
                     SASLGHNLANLNRTPEESVAGFEESVALAEQHGIKVSGSISMPFGSPWEGNISLESVK
                     SIVEAYLNVGIDEISLSDASGMAVPNQVYSMCINMQECYPQVKWWLHFHNTRGMAMAN
                     ILAAMQAGITQFDSSFAGLGGCPFVPG"
     assembly_gap    17465..17564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17565..17803
                     /locus_tag="PKU88_05840"
     CDS             <17565..17803
                     /locus_tag="PKU88_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HPX68841.1"
                     /translation="TQFDSSFAGLGGCPFVPGAAGNVSSEDVVHMMDEMGIETGIDIV
                     RLIRLSGKVRQMIGHDTDSYILKAGRSKDLIKEQ"
     gene            17911..19383
                     /locus_tag="PKU88_05845"
     CDS             17911..19383
                     /locus_tag="PKU88_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005903036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPX68842.1"
                     /translation="MSRMSSMEEVYSKLNQDQIDEIEEKFARADAAQKIFEKWSQEEV
                     DRTIRAIAWKVANTQTFKELVKMSIEESGMGDPVSRENKRFKIRGVLRDALRQKSVGI
                     IEEIPEKGIVKYGKPVGVIACVVPATNPDLTPAGNAIYALKARNAIIFSPHPRAVKTG
                     GRTIELMREGLRQVGAPEDLIQILGVGKAKINKAMSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAFGVGAGNATMIWDETAKDELHQAAYNTMLSKTSDFGSGCSADGNIVIHESI
                     YDDAIEELVNVGGYLLSEEQQLAVKKIMWDEECHRLPDSVAQSPQRMAELAGFTIPED
                     RKFLIAIGTGSYTTSYEDVWCREKLTTLLAVHKYEGEFQNAIDIIMAIHEVGGKGHSV
                     GIYSYDEEHIHRLALEARVGRIMVRQPQSKSNAGSFTNGMPMTSSIGCGTWGGNATSE
                     NVNLRHYMNVTWLSKAIPEDKPSDEELFGEFYEPEREGRV"
     gene            19422..21215
                     /locus_tag="PKU88_05850"
     CDS             19422..21215
                     /locus_tag="PKU88_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPX68843.1"
                     /translation="MVNYLQRRGVEYVFGLCGHTVIAFLDALSRSSSVKYVSFRNEQL
                     AAMAADGYARIKKKAGVVMCHLGPGIANTVTGVANASFDSIPMVVIAGDVPRYYFGKH
                     PHQEVNLHADAAQYEILKPIVKRAWRVDDPEAMPEILDKAFRMAESGRPGPVLISVPM
                     DVFSEEVEEDLFARTYLDRHQTIKPALAPKAAKAIAQKLVEAKNPVIHAGGGILLAGA
                     SKELQDLVEFLDIPVSRTLMGQGCMSDTHPLMLGQTGFWGLEFTHKFTANADVLLGLG
                     TRFAEADSSSWYPGVTFDMRKTEFLQIDIDPTEIGRNYPVSIGAVADLKEALAQILEE
                     AEKLCPEGIKRPELRERIKKNRTLFRESNIAISEDGRFPMTPQRILKDVKETIPENTI
                     IFTDVGWNKNGVAQQFDITLPGTIHHSSGLATMGFGASALLGGKLAAPDKVVITLVGD
                     GGFGINPSSLATAVEQGIAVIWIVMNNSAFGTIAGLEYSNYKTKFGTVFTTPDGKPYS
                     PNWAEVAKAYGVDAIRIDSAEAFKPALEQALASNRPFLLDVPMENIGVPTPGCWNIND
                     IYTPKANIVNGKIIKNEKGEYEAPNHSGSHK"
     gene            21242..22063
                     /locus_tag="PKU88_05855"
     CDS             21242..22063
                     /locus_tag="PKU88_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPX68844.1"
                     /translation="MPRKFSLAYLTIPGTGPVDQIRIAAEAGYDFVSLRPIPMHLPNE
                     PLFRFDQDKKLFNEIKHALSENNIRLLDIELARVREDLKVEDYESAFFAAAELGGTDV
                     LSSIWTKDKAYYYREFAKICDLAAKYSLRVNLEFVTFAGVTSLVEASDVLNTVERPNA
                     YLMVDTLHAHRSRVSAEDIAKVEPSKFGFIHLCDGPGPIPALDDPGMIGVAREGRLYP
                     GDGEIDLKSMFLAMPANPISIELPNSKEMEKRGAFGHAAQCLIKAKQYFAENGIE"
     gene            22116..22994
                     /gene="aroE"
                     /locus_tag="PKU88_05860"
     CDS             22116..22994
                     /gene="aroE"
                     /locus_tag="PKU88_05860"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPX68845.1"
                     /translation="MPVNINTKMITLLGKPLSQSYAARMQNAAYRAAGIDMLYFYTEV
                     ENDHLPDVINGIRHMNFAGFAVTKPNKVEVMKYVDEVDPLCEKMNASNTVVIKNGRLK
                     AYNTDGIGFLRSLKEEAPEIDISESVFFCLGAGGAARAICSVLAYNGAKKIYIASRTL
                     SRVKELVEDINEKFAPVAEQVDMQDTELLKKSIAASNVIMNNTGLGMPASLDKTPIPK
                     EYLSPVQLCFDATYNPSKTRFLTEAEQVGCKIINGLGMSLYQGAEQIELWSGVNAPIE
                     AMRQELLDILSGKEEK"
     gene            23045..23623
                     /locus_tag="PKU88_05865"
     CDS             23045..23623
                     /locus_tag="PKU88_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPX68846.1"
                     /translation="MNGLKWLNKHLEEAILVFLLATISCVMMAQIIARTFFNSMTWPE
                     EFSRYCYIWTVFLSLGYTIKKGNMLKVGIVMDLLPQKLRKRIEIIVNVIMLVLFVILL
                     RYSIIYTGKVKSSGQFSPAMHLPMWIMYMSTIIGFALAALRMVQEIISNIRNFNKKAE
                     TTLEATIKEAKQEVQATGIAVNDNDGLMGGED"
     gene            23623..24906
                     /locus_tag="PKU88_05870"
     CDS             23623..24906
                     /locus_tag="PKU88_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPX68847.1"
                     /translation="MALWIFVVFLSALVFAVPIAVSMVIGAMTPLFLGGTGSSIQQLI
                     ANSFSGSDTTPILAVPLFILGGVLMAEGGISRRLFNFFAYFVGNLTGGLPCAVILTCL
                     FYGAISGSGPATTAAVGSMTIPFLVDMGYDKTWSAGMVATAGGLGVIIPPSIPFVLYS
                     LATGVSTGALFLGGVLPGILIGLCMMVYAVIYCKRKGENKERINAKMTELRSKGFLSL
                     LKESFWALLSPVIVLGGIYSGIVTPTEAACISVFYSLFVSLIIYKSFTIKEIIPFLGN
                     AVKTYAPLCFVLAFAIAFGRVLALAKAPILIENFILNNFSTSITALTGIVVVFLLLGM
                     VMDTGPAIVILSPILLPAVQALGVDPVHFGVIMVCCLSIGLATPPFGLDLFVAGTLSS
                     LPPMKVAMKAMPFIIAFGIALFIITYIPWFSLVFL"
     gene            24953..26035
                     /locus_tag="PKU88_05875"
     CDS             24953..26035
                     /locus_tag="PKU88_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPX68848.1"
                     /translation="MKRLFILLLVLVMALVPAACAKQAPPAEQPAEQPAAEEPAEGEN
                     KYASLEPITLIGADNAGVGAAAQLYGELVTKKVAEITDGKLKIDYFPNSQLGNDQELQ
                     AQMLAGDIDFVIAQTAQTVSFVPEVAIFDLPMVFAKYDAKTIDYALNKSPFAAKINEA
                     YKAKNMRIMHYLQGGTFRETTSNKKISSIDDFKGLKIRTMENQNHMAFWQALGAAPTP
                     LPWPEVYVSLQQGLVHAQENATDTCVGANLQEVQDYLIMTHHILYCNQFLINSEKFDS
                     LDPLYQAALQQAVDEAAVEIEAKLTTIDKDNKQKLLDGGMELVEFEASFIDQVLEKAK
                     GVYDSIGKAIGQDYVDSLLDALENAK"
     gene            26306..27544
                     /locus_tag="PKU88_05880"
     CDS             26306..27544
                     /locus_tag="PKU88_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPX68849.1"
                     /translation="MSNTPKQIKAINVFSKGTIAAIIIITVVGLSILGVFRFTRPIPA
                     AGTSKTSENIVLPGELSVTFPEQGQAAVGTDTFGVIAASPNQESIPIASVAKIMTAYL
                     VLKAHPLQPGEEGPSLTITDEDVAGYKYAVQNNHSYLPVAAGTALTERQLLQGLMLPS
                     GNNIADTIGRWIAGTDEAFIDKMNETAKALGMTNTHYADASGADDATVSNAADQIIMA
                     QAAMQDPAFREIVAMPQAVLPVAGKVYNVNAMLGKHGIVGIKTGSGVIAGGNFVSAAP
                     IENGSEKHYIIAAVLGSRKPNENLKSAFDANAQILNQVRPQFKTFTIEPPENGFGKII
                     TPWHTESELTVNEPIQVFGYPGMKMDYSIEPLELKLPALPGTDVATLTIQTGKTTQTY
                     MMQNKVQIEKPGFLWRLIRN"
     gene            27779..29227
                     /gene="nhaC"
                     /locus_tag="PKU88_05885"
     CDS             27779..29227
                     /gene="nhaC"
                     /locus_tag="PKU88_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPX68850.1"
                     /translation="MFLEKDFKRPSFLVSLLSVVFLFIMIIAQLIAVGSPDIHLTLVF
                     AITLTVLMLMTTGTKWEKVQEGVMHGCEIATIPMLILMLIGMLIPTLIASGTIPTLIY
                     YGLKIINPSVFLLTATLICSVASICTGSSYTTGGTFGVAFMGISLGLGIPPAITAGAV
                     ISGAVIGDKISPLSDSTNLAAGVTETDLFAHVKSMFYTTVPAFVVSLILYGIIGARYS
                     ASSIDTTVVDQILAGISQNINITPMYALISLIPLIAIVVMAVKKVNALAAMVIASIIA
                     MVLAMINQGYSIIEMMAFMNYGFSIDTGVAEVNKLLNRGGIQSMMWTVSLGYLGLSYG
                     GILEKTGIMEVLLNKMSALVKNARNLIITHVFAALIVNLISASQYVSIIIPGRMLLPA
                     YRRLGIRSDVASRTCEDSGTVLSPLVPWGLCGVFFTGTLGVNTLEYMPFAFLCYLTPI
                     VAIIYAITGKFIWYEKDGMIKNDVKKAAEISG"
     gene            29227..30447
                     /locus_tag="PKU88_05890"
     CDS             29227..30447
                     /locus_tag="PKU88_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPX68851.1"
                     /translation="MKIGFGKGIKMYEIEKGQLTELLTDLVSIESPYLEEDEIMEYVY
                     SWFRKNGIKAFVHHYNEAKVTGFKGKNIVANIEGNKNGPVICLNGHLDTVKLCNGWTH
                     NPKGEIKDDRFYGVGALDMKSGCAATMIAVTAFAENHKDFRGKIIATYVSVEEGPYGM
                     GTNALIDEGYLKDVDFSISTEPSAGFSGNKFPDVCLGARGGYGLDIEFFGISSHAATP
                     ELGVSAAEDASTVISELKNIEYIKDPHLGTGNACVIGIECDGGACSVPDYAKVKLFWH
                     IVPGENEETITGEIEKAIRRASIKCKYKINFRKSPSEGSKGFMPYIVSKEDPMVKEFL
                     ETVEEVCKTKPEISYFQSIGDFNYLGSRLGVPVVIFGASGKNFHGKDEYVELDSVVKT
                     AETVYRFLERMLCL"
     gene            complement(30481..31680)
                     /locus_tag="PKU88_05895"
     CDS             complement(30481..31680)
                     /locus_tag="PKU88_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HPX68852.1"
                     /translation="MLSINFLGKPRVEYNGKKLEEQLGSKAVVLICLLVLNEKRYMSR
                     EKLEGYLWPDSSTDAARYNLRYNLWLIKKNIGTDHKGHKFLHVDSDCCSINKEYEFEC
                     DTFEIMSFKHSQNDSLESILKLKQLFRGDLLEGCYFKNCDELNDLIIFERMKYEQRKV
                     KILKSLVDYYEKEESFDNCLEVINEILEIEPYDEEMVLKILDIYVKCRKPVAAITYYN
                     NFSKLLTSSLGISPSNQLRKKYEEIRSVLSDSIDNNSSKANMDTFMKDISKQENVSVI
                     EIISDCIKNVDYFWISDVVGKIIEAVGTDYLNQLSEKELLDLAYIQCDILYYCDDKFE
                     HIKDYGRDTMSVCIINAFVKLLRIICSKQELTIIILNNTDMDDTSLNVLKYLKRIKIE
                     GLKLVEE"
     gene            complement(31700..33232)
                     /locus_tag="PKU88_05900"
     CDS             complement(31700..33232)
                     /locus_tag="PKU88_05900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPX68853.1"
                     /translation="MHASILIKNGRCMTMEGKEEKKWIAVEQNKIVAMGDNTEYESLI
                     DGDTIVFDAGGNTVLPGFIDSHFHVIQTALDSISVDLSYAKTYEELGEQLREAASSFT
                     GGYIRGIRISEEQFTEGRFPGRTLLDKYCSNVPVVLNSMEYQVSILNTYAMLYFKIPF
                     TAEGIELDRKQMPTGIFRKQANAILRTNILKNISDNIRKDAVEGIMGTLLMNGITTIN
                     AMEGGFMYSDKDADFLYEHGKEFPIDIELFYQTMDIEKIKAMGLKRLGGCLYVDGTFG
                     ARTAALSFQYADCPGTMGSLLLTQQEINDFVLNCYKNNLQLALYTIGDRAIETAINAH
                     ENAFYQTGIIGLRHRLEHAELINEKQIQRAKELGILLSMQPSYETYWGGPGKMYEQRL
                     GDKYILTNPFKEIIEKGVIICGGSDSDVTEPRPLLGIHSAVNHPVVKHRVDIYEALKM
                     FTCNGAYGIFQEKNKGTLSVGKTADIIILDRDILLVPSHQIEKARVCVTIKSGETLYS
                     NL"
     gene            33484..33936
                     /locus_tag="PKU88_05905"
     CDS             33484..33936
                     /locus_tag="PKU88_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPX68854.1"
                     /translation="MERIVKGGIYHVIARGNNKEYIFKENVDKGYFIKQIKETTAGMG
                     YKVFGFVLMDNHYHIIVQTFDKKLQDIMHQINNKHSKYFNYKYKRVGHVFQGRYKAIL
                     VQDEKYIVGLLRYVHQNPVEAHICNDMDKPGRLLSVKVRFKEKNIDSI"
     gene            34334..34828
                     /locus_tag="PKU88_05910"
     CDS             34334..34828
                     /locus_tag="PKU88_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPX68855.1"
                     /translation="MSENKTLDNLMAAFAGEAQANRKYTAYAKKAEKEGKMNAAKLFR
                     AAADAETLHALKHFEVAGKIGSTAENLKDGVAGETYEYKDMYPDFVKVAEAEGNKAAL
                     MSFTFAMKAEEVHAGLYQDALENIDETEEVFYYLCPVCGNIEKSRPEKCSICGVSGEK
                     FIKY"
     gene            complement(34914..36200)
                     /locus_tag="PKU88_05915"
     CDS             complement(34914..36200)
                     /locus_tag="PKU88_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX68856.1"
                     /translation="MSIRFKLPLLVLAAFLINIMLLYGYYNFYLSREIAGYNSNMREQ
                     LQTETDRIIKRIESSQDFSQALQIISQTENLNIQVADESGRTVFHGGKDGGVSIEINS
                     SGIFHHDNKVYLLKVTQPVSLKKISSYYVVWNLFIAEIFIICSILLFIAAIIYFNYVK
                     PIIALQKSMERYKEGIHPQNVARKDEIGLLQNRFVRLTEAIEKEKQKQNLIIASISHD
                     IKTPLTSVMGYAERLKKNNLTADRYGRYIDIIYNKSVSIRDLIEEFDEYLNLQMQSGL
                     KQQRISAEKFCAILKSDYEEELAERGVSFSISVSCPDKILFIDIAKMRRVFGNIIGNS
                     LKHFSVREPSIAVFCSKQENCIIFSVEDNGTGVSEGELQKMFDPFYTSDKSRSVAGLG
                     LSICREIVEACNGSIWAENNESGGLCVKIILPHAAP"
     gene            complement(36190..36885)
                     /locus_tag="PKU88_05920"
     CDS             complement(36190..36885)
                     /locus_tag="PKU88_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68857.1"
                     /translation="MEKILIVDDDREIASLISDALTDEGYQCILAFDGEHAFTLLKSI
                     QDFSLIMLDIMMPGIDGLEICREIRKTVSCPILFVTARNRTYDAMLGFEMGADDYISK
                     PFVVEELVARVKAHLRREKRSLQKNNSIMTIGGISINPESYEVTVNGRAVELSMREFQ
                     LLHYLFINAGKVVSKEQIFSAIWGADFTDIGTVAVNIKNLRDKIDKDNRYIKTVWGVG
                     YKLIKHQEKENEY"
     gene            complement(36890..38077)
                     /locus_tag="PKU88_05925"
     CDS             complement(36890..38077)
                     /locus_tag="PKU88_05925"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPX68858.1"
                     /translation="MFLRHKKSILIGIGILIMLTAAVIISFNAAGADRAQSFETVSSS
                     SEQDNRLDFWGEVKYERIYDISIDFPAIVTDIKVKEGDHVSLDQVLVTLDMSEYLGTV
                     EKLQHQLEAGQAGLQSIRKDTGALEADIQQIQEDILEKTEELAKETNADLKILKTSLN
                     LAKKEAENAKRDVSSNQALYDAGAVSGNTLDRYMDILAEKEKALKDIETGIEKTKSAL
                     RTELDQLSILLKSKSAQLKEIKDSNISNTARQESGVAVSRIDLEIMKSKSIKDYLDSD
                     QVISCVPNGIVRNIEVVNGTRLGVQNNPTRVLQLIDADSIIVIAEVEEEFIRNIALDD
                     SVEIVPASDSSTSIPGTVIQISNEAVEKDGKRIIKVQIKPEDPGMVLKPGYSVDVYAN
                     RKE"
     gene            complement(38106..40415)
                     /locus_tag="PKU88_05930"
     CDS             complement(38106..40415)
                     /locus_tag="PKU88_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68859.1"
                     /translation="MVMQSLFIKSLRDMQKSKVQFLSILIMATLAVCIVTGIDSIWKT
                     VELHSESMYAAANISDLWVNVLNPTEKELWGISRIEGVEKAEKRFVLDALSDLENSPT
                     LRVYAVSGKGVLDRPQLLSGKLSSRSGAILDEVFARMHGLDIGDDISIKLNNKWIRFP
                     IQGLALSSEHIYSVKGSTDTFPDPKKYGFIIINEDMLKNAYGRKIYNQISVKLSPGAD
                     ITKVKTHIFNAVGDDLAGIVAREDHRSVSNINANIQQFKLLAAVFSLMFFIVTALITQ
                     STMMRLVENQRGQIGILKALGYGKKSILWHYTSYGVYMGLLGAFLGLLSGPVIFGKML
                     IPWLRLNFTDYKISINHSNFIISLVLILLCTGGISLYSCLRLLGDSPSVLLRIKPPKE
                     GSHIFLEYLSGLWSRMRFSRRLIARNTSRNKIRLIMSILGITGCTGLIVAAFTIINMI
                     NGIARHTYNVTYTYDHKILLDSKADSRFIHNMGLEGTVQQIRETAAEIICPNGERQLE
                     LLTVSPQKSPLIRLKDVEGNPVLLPDEGAAITRKLADTLKIKAGDIIELKRTNKGYIR
                     VPVKQIVYLATGQGMYMSDTYYESIGEIFKPTAILVKWDNGPDWSFLRGDYAEEYVSR
                     ADQIADVKSSTRVVYIAGVMLIIMGGILAFVVLYNSSILNFSERIRDLTTLKVLGFYQ
                     KEIRSLVLTENTLSVIAGLILGMPVGKVLAEIVAKGLNDQMDLIGNVSFATVVFSVAI
                     TAVFALFINSIAAVKMKSIDMIESLKSVE"
     gene            complement(40409..41110)
                     /locus_tag="PKU88_05935"
     CDS             complement(40409..41110)
                     /locus_tag="PKU88_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68860.1"
                     /translation="MSFITFENVEREYHVGGNIIKAVDGVSFGIEKGTVNVVLGQSGA
                     GKTTVLNLLGGMDSPTKGRIIADNLEISGMAEYELTTYRRNKIGFVFQFYNLVPNLTV
                     LENVLLAEEICKDPLDAKEMLIKVGLKDRMDNFPSQISGGEQQRVSIARALAKNPQIL
                     LCDEPTGALDSATGRIVLKLIRDAARDMGKTVIIVTHNVLISEMADVILHLKDGKISD
                     IRYNSSPKAAEEIIW"
     gene            41512..44241
                     /locus_tag="PKU88_05940"
     CDS             41512..44241
                     /locus_tag="PKU88_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPX68861.1"
                     /translation="MLKRKLYVNGVARTVIADPEASLAQYLRKQLCLTGTKIGCGKGE
                     CGVCTVILNGKAARSCIVKMKNVPDESKVITIEGIGSHENLHPLQLAWMVNGGAQCGF
                     CTPGFIVSAKALLDQNVNPTRDEVREWFQKNRNVCRCTGYIPLVDAVMDAARVLRGEI
                     KMEDLWCKLEEGASMLGSKEPRPSALAKVTGTWDFGADLGLKLPENTLHIKLVQATVS
                     HANILSIDTSEAEKMPGVFKVITHKDVPGTNRINGLAFPQNKGDGKERPILNDKKVFQ
                     FGDALAMVLADTPTQAEEAAKKVKVELEVLPAYMSAPEAMAEDAIEIHPGTPNIYFET
                     HRIKGEDTKPLMESLPYVIEDDFYVGRQPHLVLEPDVGFAYLNEEGKLIVHSKSIGLH
                     LHHLMTAEGIGIDPDKYILVQNPAGGTFGYKFSPTNEALLGVAALVSKRPVFLEFNMY
                     QQITYTGKRSPFFIHLKYGADKDGKLKAMEADWSVDHGPYCEFGDLVTMRGTQFIGAG
                     YDIPNSRGVGRTVATNHAWGSAFRAYGSPQSLFASEVLADMMAERIGMDPLEFRYLNV
                     YREGSTTPTGCAPDVIAFPQAIDTLRPLYKEAKERAKGKNKNGGNIKYGVGISLNMYG
                     CGLDGADSSEIWVELTPEGVTVGTSWEDHGQGADMGTLTYTYEALRPLGIKPENIKLV
                     MNDTEKTPNSGPAGGSRQNVVTGNAIVAGCRKLLEAMKKPDGTFRTYDEMVAEKLPLR
                     YEGKWTAPKTACDVDTGQGDPFSTYMYGVLMSEVAVDTTTGKTQVEKMTIVSDCGTIV
                     NKLVLEGQIYGGLVQGIGLALSEDFEDLKRHTTLTGCGIPQILDTPDNIEIIHQETPR
                     PYGPYGASGVGEMPLSAPHASIINAIYDACGVRIKHLPAYPEKVLAGLKELNK"
     gene            44326..>45720
                     /locus_tag="PKU88_05945"
     CDS             44326..>45720
                     /locus_tag="PKU88_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX68862.1"
                     /translation="MDLDKLLETSDSCIHDEPPACTSCCPVHMDVIAFVGEMEKGDFD
                     KAYKVMEKRVPFARIIGSICDHPCEKVCVRSDSGGTVKISELEKAAVSYGFSPPKNTI
                     PLSKSKGRVAVIGGGISGMTAAFELDKKGYQVTLYEKSDRLGGRIWDYEGIALKKEVI
                     EEEIAALVKQGIKAELNTLINKKRLEELAGEYDAVYLGTGVWEEELKIHPETFQVGDT
                     SVFAGGKLANNNDSVIFSASSGRRAAASVDRYVKKISLSASREREGSFETPLKYETEG
                     IEPVEAIGKTSDIYSEDEAIGEAGRCLKCRCRKCIESCSHLTRFNISPKSYMRQINQN
                     ERIILGTHYANKMINSCTMCGLCREECFKGISMKAVIKETRESMVRRGKMPVSDHDFA
                     LKDMKFSNSKYFSMVRNQGEKAEYLFYPGCQLPASYPEYIGKIYKYLVSNMSGGAGIY
                     LGCCGAPADWAGRQD"
BASE COUNT        13573 a         8900 c        10289 g        12858 t
ORIGIN      
        1 tgccttccag cggcttagct ggtttggatg cacctcatat tgagcagcaa tctcattcag
       61 tgttttttct tccctcagaa cctcaattac tatctttgct ttttcttccg gtgtgaaatt
      121 tcgtctcttt tccataccac tattctaact tacttctgct gttttgtttg tcttgttttt
      181 tggtatcatt attagtttcc aatctggtga attttgttga tgcaaagacc gggttcttgg
      241 ggctttggag aaacgaaagg cccggcctta ttctccataa cgattacgga agacagaatt
      301 gacgaattgg aggatgataa attttctgtc gagattatta ctgattgtgc cagctacagg
      361 attcccgttc catttataaa aactcaaaca gaatgatttt gttcccggtt ctgagggtat
      421 aattaaagtt gcagaagcat cagatgaagc agtgcaattt aagcgaggtt ctatgcgaac
      481 tggtggtatt tccttgactt tgtatccgat ggaatttcat ataacggtta tgcatgaaga
      541 taagattata gatacaatac gtattgagga ctttgtgaat aggatcatta ctgttcctga
      601 aaagtttagc gacagagatt ggaagcatca aaaaaaattt taattataca atcaaagggg
      661 ttaggatggc ttaaccccct tttgttttca taattgcttt gttctcagca caggaatagc
      721 atgatggaag aaatgtggct gaggctgccg catatcaaga tgatgaagga tcagaagccg
      781 caggtgatgt aaactgggaa ttctgcgcag cagattagtg cagggctgcc aagtacttat
      841 tatttgcttg aataatgttg tttaaaagaa taaaatgaaa ttgtcggaat gtatcatact
      901 atacgattat taaaattgaa gctggataaa tatgcttaat gctgttttta gtttattaat
      961 tattttttga aagattccgg taaagttatg tcagaggttg cataagactt tgcggaaaaa
     1021 taaatttttc ttgctgatac tattaagaga ggtggataaa aatgaaacat gaattccggc
     1081 ttggtttgga ttgtgcaaaa gaattggata tgcaggaccc gttaaaggag gtccgcaacc
     1141 gcttctatgt aagaaaggat gaaatttaca tggacggcaa ttctcttgga ctttgctcca
     1201 aggatgcgga aaaagctgtg ttgaaggtta tggatgtatg gaaggaacat ggcattggac
     1261 tttggaatgt tgaagacagc aaatactttc tttatccttc ctatttagga gggcgtatgg
     1321 cctccttgat aaatgctgaa gccaatgagg taacagtaac aaacagcgtc acaatcaata
     1381 ttcatcaagg gattgctacc ttctataaac cgactaagga taaatacaaa atattagtgg
     1441 atgatttgaa ttttccaaca gaccgttatg cagtagacag ccaggttaag ctgcatgggt
     1501 acgatcccgc tgatgtggtg aaagtagttc aaagcccaga tggcagaata atttcagagg
     1561 atgcggttat tgaagctatg actgatgatg tggcattgat tctactaccg tctcttcttt
     1621 atcggagtgc acaattgctt gatatggaga ggataacaaa ggaagcccac aagcgaggta
     1681 tttacatagg ctgggatctt tgccattcca ttggcgttat tcctcatgat tttaaaaaaa
     1741 tcaatccgga ttttgcagta tggtgtaatt acaagtatat gtccggagga ccgggaactt
     1801 ccgccggatt atacattaat aagaagcact ttgcgatgga cccgggatta acaggctggt
     1861 tcggaaacac caagtccacc cagttccagc tgaagcacca atatgagcat tcaaatgatg
     1921 ctgacggttg gcttacgggg acacctccga ttctttccat ggcagcaatt gaaggagtac
     1981 tggatattta tgatgaaatt ggaattgtca ggattcgtga aaaatcatta aaaatcactg
     2041 actatctaat gtatcttata gatgaacgcc tcacaaaata tggttacagc attggcaatc
     2101 cgcgggaaga tgataaacgc ggaggacatg tggctttgga gcatcaggag gcttaccgga
     2161 tctgccaggc tttaaagagg cataaagtaa tacctgattt tcgcgagcct gatgttatta
     2221 gattggcacc tgtagcttta tatgtttcat atgaagatgt ccacaggctt gtagatatac
     2281 ttgaagaact caccataagt aaagaatatg aagatttcga gcatacccgt tcccttgttg
     2341 tataggcatg cagaagggaa aggggttcaa atccctctct ctccgccaaa aataattgat
     2401 tttatataaa atagcactgt attctggaaa cgaatacagt gcttattatt caaatgccca
     2461 aacctccggg ctatccattt catactgaag tccttccaag tcacccatta agattctttc
     2521 aatattgaca ccaaatatgc ctgaaaggtc gccgagcaga gtcacatcgg gacagcccat
     2581 gccacgctcc cattttgaga tggttttatc gcttatattc attctgtcgt caacatcttt
     2641 ttgggtcatg tttttttctt tatgcaagct cagtatcaat acgccaactt tgctacaatc
     2701 catttaattc cccctctata tatctaatat aattttcaga atttaactat tcaataaacg
     2761 ctccgcagag tttttgagaa tatgaaagcc tggttgttct tgctatagga aaaatggtat
     2821 taaaaacgtc aaaatagtac aaaaactggt aaaatatgat attattattt tataagcttc
     2881 atggagggat tcttgtgcaa aagacttata ggtttatgaa agatactaat aatataagac
     2941 gaataaagta tagatcggag aacaatagtt tttttgatga aaaggtttct tatattattg
     3001 actcactgga tgatgcacat aaattaagtc agaggagttt tttacagagg gtattcgaag
     3061 cgactttcaa gcttataccg gaggcagaaa aggggtcttt ttatgagttg aatgatgata
     3121 aatatacgcc tatattttca atgaactacg attttgagtt gttaaaaaaa ctatcgttta
     3181 ataaagatga cgcatttatt gattttgagt gcgatgatac ttcgagtatc gatgcttacc
     3241 aggtatatat atcaaaacgt gatgatagta agttctctaa agaaatcaag aattacagtc
     3301 agcaaagccg gtcagtgtac ctgctgcgcc aaaacagact ctgctgtata cgaatatcgg
     3361 tgataccgat tacgagacgt ttgtcaaact gaagaaagcc ggagcggacg gagcatacca
     3421 tgtcatccgc ctcggtgaag gcacttatac gaccatcagt cctcagcgcc gtcaggagac
     3481 catcgacaat gcgcgccgtg ccgggctgat gctgcaggac tgtctggaac ccatcggcac
     3541 agagcataca ccggaggaac tggtggatca cattttcaaa accattgaaa aaggcgtcga
     3601 tactgcaggt gttatgaagc gcaccccggt acctgaaact tcattcacag acgagattac
     3661 aaatgcgcgt ttggcccaga tcgcttccgt aaatgccctt gcgatgacgg ccatcgctcc
     3721 gtatccttgg attcccgtgc atgaagcgga ttatatcagt cttgtcagcg gtgccaacag
     3781 catctgcgct gaaacaggag tcaatcctcg tgatacagaa gcggacacct cgaaaggacg
     3841 tggcttggat gtcacagctt gccgtaaaat gctctggcag gcaggattta agcacctcgc
     3901 caaaggtgac ggcactcgca ttgagttgac accagaatat attactaaat gtgaaactac
     3961 ttgttaatag tagtagccgt atagcgttgc acccccctcg gctattttgc acccccctga
     4021 aaagacaaaa ttgaattgta tcaatctcgt actctaaata caaaatattt gaaattacat
     4081 ataaagcact gcattttgat atgcagtgct tttgaatatg ttcgcataaa tctaatatag
     4141 ggttttttat ttgatttgca cttaaaatta aacaataatg atgtccttgg tgactttgtt
     4201 gagtatttct gccttaccgt tttccaccag aactatgttt tcaactctca tgccgaaatt
     4261 tccggaaata tatattccgg gctctacgct gaagcacatt ccgttttcga ggatctgctt
     4321 gttgccggtt cttatatacg ggccttcatg gacagcaata cctataccgt ggccggtcct
     4381 gttgaggaag tattccccgt agcctgccgc ttttataata ttccgggcag ccatatccac
     4441 ttcttctgca gtgatgcctt ctctgacggc catttcacct gcagttgtcg cttttacaca
     4501 gatatcgaat atcttccttt gttcttccgt gggttcacct acgaaaatcg ttcttgaaat
     4561 atctgagcag tagcctttat acctgcagcc ggtatcgatt attattatat cctgttcctg
     4621 tatgaccctg ctgtccccat tgtaatgagg cttcgaagta ttaggccctg atgccacgat
     4681 agggtggaag gacatgcttg cacctttttc cgtatagagt tcttccagct tttttgcaat
     4741 atctctttct gttaaacccg gttttataaa ctttattgtt tcttcaacca cttcatcggc
     4801 aatttttgcc gcttttctca tgcagtcctt ttcttcttgg gtttttcgga ttctgacatc
     4861 ttccagtata ctatttccgt ttatgaattt tacgtctaag aagtttccta tatccagcat
     4921 atttatagct acgatgccgt catttaccgc aatggttttt cctgcaaggc catatttttt
     4981 atcggctttc tcgattgctg acaggaagcc gtctgtatcg ctccatacaa atatgtcttc
     5041 cttttctccc aagagttccc gggtttcttc atagtatatt tccggagata tgtagaaatg
     5101 cctcttatct tttaggatga acagcgcttt gaacctttca tccggatgag gattgattcc
     5161 cgtcagatat tcaaggccgg aggaagggcc aatcatatga atatccgcat taatatctga
     5221 cataagtcct acaatttttt ccaactgttt ctctaattgc atgatttcaa ccccttaaaa
     5281 tttatttgta ttgttccaat tttattatag tactctttat agccgaatta caatattctt
     5341 caatataatt tggaatgccg gcaatgggtt gtccttttga taatactaag ataatatgtt
     5401 accaggagga cttttttatg aaggacggca aagagtatgc ccttgggtac aatcaacttg
     5461 gaaaacaggc aaaggcagca ggaatagatt atgtcggtgc cggacacaaa aaggcaagcg
     5521 gataattttg gcggccggcg ctcataaacg aaaaaatatt gaaagcaggt gcatagcatg
     5581 gttcatagat ttgaatccca cactcccagt ctggatacta aatcttttgt ggcggaaggt
     5641 gttcagctta tcggcagagt agtgatgaag gagtatagca gcatttggtt taattcggta
     5701 gcaagaggcg atgtcaacgc tatcgagatt ggctgctata gcaacatcca ggataatagc
     5761 gttattcatg gcacagatga ttgtgcttcg attataggcg attatgttac cgtcggacat
     5821 ggttgtatta ttcatggagc aaccatagag gagcattgcc ttttgggaat gaattcagtt
     5881 attcttgcag gaagcgtcat tggcaaggga agtatagttg cagccggaag cgttattctg
     5941 gaaaacgaga ttataccgcc gcattcgctt gtggtggggg tacctggcaa ggttattaaa
     6001 caaatacccg gatcgtggga gcagattcat gctcaggcta taaaatacaa aaccttgtgg
     6061 accgagcgtt atggactttt gcccaatgca gggggagagc ggtacaacgg tgaaaagatt
     6121 gtataatttg cttaggtgcc ggatacagaa taatagaact taagcgatgt atcaatttag
     6181 ataagttctt agattttgtg cctaacacaa agttaggggg taatgggatt ggcagaacaa
     6241 aaagcttcat caaaaaaggt gaccctctgg acgaggcagg atataaggag cctggaggat
     6301 ttgaaaaaag agggtgtcct tcgtatcaaa agaaagcatc tggtagaaaa gtttgatctg
     6361 attgcagact atatcatcaa tttatacagt tggtttgtta atgctgcaga taggatggtt
     6421 cccaagccgg agggagtgga gtttccgata tggtgttcca ttagtgagga atatatgctg
     6481 aggcccacgg acactgagat agtgtatgtc ctggaggtgg atgaatcgga aataatatat
     6541 tttgacggct gtaaatggga ttatgtgctg aaccatcatt atgtgccgaa ggatgataag
     6601 gatttggaag agtatgaaag ggatatatcc gataaaggct tcagggattc atattctttc
     6661 cttgatgaga atacttcaca tttttatccg gaggaaagaa aaagggtaat ggacagttgg
     6721 ataagagtat ttgagacaga tcaatgggat cctttcagga tacaggcaaa catgtgggag
     6781 ataaggcctg aaatgattaa aggcatactt aaaaaagatg tgactataga cgccatgttg
     6841 tctcagatga ttaaatgata taatagttat aagggaaatt gacttagggg ataggaggaa
     6901 aaaaattgag tactgctccg ttatcaaagg aaaggtccag agcaatgctg ctgcttgctg
     6961 ccactgcagc gatgtggagt atcggaggac tgcttatcaa aattgtggat gccagccctc
     7021 tgtctattgc aggagcaagg tctgccatca catcaatagt tctgcttata tacctcagaa
     7081 gaccgaaatt taactggtct cttccacagg taggagcggc gatttccagt tcagctacag
     7141 caatactctt tattactgca acaaagacga cgactgcagc caacgcaata ctgctgcaat
     7201 ccactgcccc aatatacgtt gccatttttg ggttttggct gcttaaggaa aagaccacaa
     7261 aatacgattg gctgacagtt gccgtggtaa tgggggggat gggattattc tttgttgaaa
     7321 acctgagtac cgaaggactg ttggggaatt tttgcgcatt gctgagtggt attagttatg
     7381 cattttttgt gatttttatg aggatgcaaa aagacggttc tcccttggaa tcggctctgc
     7441 tggcaaatat tattaccgct cttattgggc ttccttttct ctgccggtct gtaccaagca
     7501 cttcaggttg gcttttcctg ctgatacttg gagtatttca actgggcata ccccatattc
     7561 tttattcttc agctataaaa aaagcgacag ccctggaagc ggtgctgatt tccatagttg
     7621 aacccatact gaatcctgta ttggtgtttc tggtgctggg ggaggctccc ggtatttatg
     7681 cattgttagg gggtgctatt gttttggttt cgataacctt aaaatgtgtt cttacaatta
     7741 tacctttgaa gatagtgtcc gaagacaaaa aggaacagcc tttggaataa gaggaggcta
     7801 agcatgttat acctaaatag acaagatatt tcttcactca ttgatcttaa tgaatgattg
     7861 aggccttaga acactctgtg gaaatctatg acaagaagca atttatgcag ccggatcgca
     7921 taaaagtcaa tgtgaccgat acggagacat atctctatat gccctgcttc accgaataga
     7981 taaaaggaac caggatcctt accctgtccc aggagaattc caaatacaat ctgccgacgt
     8041 acagggggtc atgctgctcg acaatccaaa aaccggcaca atagattgta tacgacctct
     8101 tttactcctg tgctgcctga tgacgctgaa cttttggaag ggaagcattt tataggcatt
     8161 ggatcttaca aacccaatat gcgggagtac tcggagagca tttacaaatt gcttgaaaag
     8221 atttacatag atgttgactt tgccagggag gaatcgggag atttggcgac gccttgtgaa
     8281 aaaggctggt tcaaggaaga aaatatccgg acactctatt cagttgtcaa agctgacagt
     8341 attgaccgca gcaggactac gttctgcaaa tctgtcggaa tggcgctgtt tgatattttt
     8401 gttgccggaa atatatataa taaggctata gagcagggta tcggccaaaa attaaatccg
     8461 taaccgcaaa gcaattccaa taaataacgg agggtttttt atgatgaatt tttcccacta
     8521 tattacatgt attgacgcac atacggcggg agagccgctg cgcattatta cagcaggttt
     8581 tccgcccata ccgggcaata caatccttga aaaacgcgac tatgtaaata agaactatga
     8641 cggactgcgc cgtatgctga tgctggagcc ccgcggacac tcgggaatgt atggatgcat
     8701 tcttgtacct gctgttactc cggatggcga tttcggagtg ctgtttaccc acaatgaggg
     8761 gctcagttcc atgtgcgggc acggcattat cggcgtgacg aaggtagtaa ttgaaaccgg
     8821 gatgataggc tgcacagaag gtgagaatgt gataagaatt gactctcctg ccggccgcgt
     8881 tacagcgtac gcagatgttg agaaggggaa ggtgctgcgg gtgcgctttc ataatgtgcc
     8941 ctgttttgta tataaggaga atatcactgt acatgttgac agcctgaaca gggatgtagt
     9001 ctgcgacatt gcctattgcg gtgcgttcta tgtgtacatt gacgccgaca gggttgattt
     9061 ggaagtagag ccccaatacg ccgagcagct ggtgaagatt ggtatggaga taaaacacaa
     9121 agtgatggaa gaatatacat tcaaacaccc tacagaaccc ggtattgact ggctgtatgg
     9181 cactattctg atgtcgccgg tcgaacgtat cgggaatcgg ctgatatcga acaacatctg
     9241 tatttttgcc gagggacaga tagaccggag ccctacggga acaggcaccg gaggacgtac
     9301 ggcactcctg gtgcacaaga atatcatgca ggaggatgac acacttgtaa acaaaagtgt
     9361 aatagacact cctatggagt caaaagtgat aagccgctgc aaagtgggcc aatatgacgc
     9421 cgtgattacc gaagtgtcag gtaccgcaca tatcaccggc ttcaacaata ttgtgcttga
     9481 tcctgatgat ccgctgccgg aagggtttcg tatcattgga tcttagtgcc cggcactatt
     9541 atccggccaa tacccacggt agaagtatta gttttgcaga acatatgtga tgagttatgg
     9601 aggtaaacac atggagagaa aggttgaaca acaggttaga gggtttagga cacaagacgg
     9661 cgcaggagta aatttggtta gagtattggg aaaggggact gctagtgaat atgatccgat
     9721 tttaatgctc gattcctttg acagtacaaa tccggatgat tacacagcgg gatttcccat
     9781 gcatccccac agaggcatcg aaacaatcag ctatgtgtat cgtgggtata tgacccataa
     9841 ggatagttta ggcaatgagg ataccatcgg cgatggagaa gtacaatgga tgacagcggg
     9901 atcaggtatt ctgcatgaag aaaagctgcc ggcatccgaa agattgcttg gggtacagct
     9961 ttggcttaat cttcctgcga aggataagat ggttccgccg gcttaccgca gcataaagaa
    10021 tgctgaaatt gaagaagtta agttggagaa cggaaagcta agactgctcg caggcgaata
    10081 tgagggaaga aaagggtata tgagcaaata tcttccgctg gattattatg accttcatct
    10141 aaatgccgat acctctgttt ccgtaaaaac ggaaagaagc cgctcggtaa tgatttttac
    10201 actcttgggg gatgcatata tcgggggcga atcagtaaaa gaaaagacgg cggtaaagct
    10261 tacctccggt gaacatgtgg agataatagc aacaaacaaa aatgcgcaat tgttgtttgt
    10321 aagttcgaca atcctggctg aaccgattgc ttggggggga cctatagtta tgaacacaaa
    10381 ggaagaattg aataaagctt tcgatgattt gaaaaatggt acttttttgc agaaaaagat
    10441 ttcatactaa cggaatttca cccgaagata aacagatgcc aggcaccgga caaaatatta
    10501 ggcgtgcctg gcaattactt catttttctg cttcatatgt aagtatataa ttcccgtcat
    10561 atttttgata gattttctct acctgatcat taatcagttg gtcaacttgc ccattatagt
    10621 aaatacgggc gcttacacca cagtttgagc ttagtgatct tggtacgggc atcagctcac
    10681 aagagatatt ttcttttttc atagcccgct cgaattttat cgccccggta tgggtgtaaa
    10741 aaagaacttg atattctttg ttcattttct ggcttttagc acaaatgtgc cgtcgttttc
    10801 ttcgatttca actgaatacc cgacactttt caaataacgc tgaacattcc cttttgctgt
    10861 attattatct actataacat ctataccgtc tttttcctca tcaagagctt ttttagtcat
    10921 aagtaccggt tgcgggcagc tcatgccgca ggtatctatt gttttcatat ttacctccta
    10981 ttaagactgt gcgttttttg atagaaactc tgaattggcg taagagattg ccaataggat
    11041 taaaaagcag attccgacag atatctgtcc gtttagagtg actccttttg cactggatgc
    11101 cagtccaaag ttgtggcaaa tagccgctcc tacaatcaat cctaatgcgc tgatagctga
    11161 atcagtgttg ccttcgccgg caagtatcaa ctgacgcaaa ggacaaccac caagaagtac
    11221 agatccccat cctaccaatg ccatgcccag gaaattccac aatccgtccg tatgggcaat
    11281 aggttgctcc gcaaaaccgg gattaaattt acccaaaatt aagtttccaa ctaaagctac
    11341 tgcaagaatt gacaagaagc caagcatcag atagttgtca cggaaaagaa tcaagtctct
    11401 ggtaccaccg acagtgcaca acctatactt ttgcgcaagt gcacctacaa ttaatcctgc
    11461 cgcaagtgaa atccatatcg gtgctgtagc tgctcccgga ccggatttac tgaaaaacac
    11521 aaaagcaggt gctacaataa gcaaggccag aaagccgaca ttaatagccg ggaacagata
    11581 tccttccaaa tttgtaagct tataactcct cttaagattg aaccccttgt tgagaaagaa
    11641 aataccaacg taaatcccag aaacaaatcc gatcaatcca ataattgcat tgaaatcgcc
    11701 cccggcaatt ctcaaaacca ttcgaagggg acagccaaga aaaataagtg ccccgatcat
    11761 tacgatgatt gaaagtacaa atctggtaac aggtgatgag cctccttgag aattaaactc
    11821 cttattcatg gaggctagaa tgaatgaacc taatacaaga ccaataatct caggacgaat
    11881 gtattgcaca acttctgcac ggtgaagacc aagtgctccc gctatgtcgc gaaggaagca
    11941 tgcaatacaa aaacccatgt ttgccggatt gccgaaacga actagtagaa ctgcaaataa
    12001 gccaactacg ccaccggtga taataattcc tgttttttct ttcatacttc aatatctccc
    12061 ttaatggtat aattatagat aattctgtta ttagtttaac aatgcaaaca acaatagtaa
    12121 aataacaaaa acaaataatg acgtgctgta gaattatgaa aacgaataca aattgtattt
    12181 atgttttttc caatgtataa tctaaaatat ggatgactat gcgaggaggt tcactatgaa
    12241 agaaatttat atggacaatg gggctacatc ctttccaaag gccccgggtg tagctgaaag
    12301 tgtatacaaa tatcttattc atgtaggtgc aaatatcaat agaggctctt attcaaattc
    12361 ttttcaggct gaaaatgtag tttttgagac gagggagtta ctttgttcgc tttttggttt
    12421 caataagccg gaaaacgttg tatttacaaa gaatataaca gaaagcctta atgtgctggt
    12481 taaggggctt tttaaaagcg gcgaccatgt gatagtgtcc tccatggaac ataacgctgt
    12541 gatgaggccc ctcagggctc ttgaaggaaa aggggttaca ttttcaagag ttttatgcaa
    12601 aaatgacggc agcatcgacg gaaaggattt attgcctcta atccttccca atacgaaggg
    12661 agtagttatg actcatgctt ccaatgtttg cggtacaatt ttgaatttgg aagaggtcgg
    12721 aagtatctgc aaagaaaaag gtctgcggtt tattgttgat acggcacaga cagcgggagc
    12781 tataaaggtt gactttgatg atatgtgcgc tgatgccgtt gctttcaccg gacacaaagg
    12841 cttgctgggg cctcagggga ttggcgggtt tctggtgcgg gaaggcttgg ctgatgaact
    12901 ttccgcattt attgaaggag gcaccggaag cttgtctgat atggaaatac agccttccta
    12961 catgccggat aaatttgaag cgggaactcc gaatgtgccc ggtatttacg gacttaacgc
    13021 ttcactgaaa tatatacagg agacaggtat tcaagatata tatgaaaaag aaatgcggct
    13081 tatgggagtt ttcttggaaa ggctgatgaa tatgcctcat attgatataa ttgggaagaa
    13141 ggatactaaa gggagaacag gaatcgtatc tgtcgatttt aagacacagg ataatgcaga
    13201 gatggcacac cgcctctctt cagagtccgg tatcatgacc agaagcgggc tgcactgtgc
    13261 gccttccgcc cataagacat tggggacttt cccaaggggt acagtacgtt tcggattgag
    13321 ccatttcatg gaggaagccg atatattata tgcggcggac caaatatata aactgaagta
    13381 accggagggg gataaaatgg aatttaatca gataaagata tttgttgcgg tcgcccgaaa
    13441 aaagagtttt tcaaaagcgg cggacatgtt gttcatatcg cagccggctg tcaccagtaa
    13501 tgtgcagaaa ctggagagcg aacttggggt tacattaatc aatcgaaaaa ccaagaatat
    13561 ctcattgact gaaggaggaa aactttttta ccgttatgcg gtggaattaa ttaatattta
    13621 tgaaaaagcc gaatacgccc tcagcggata taaaaagaat attgaaggga ccctggagtt
    13681 atatgccagc acaataccgg gacaatatct tcttccgtat atcgttaaag aattcaaaaa
    13741 agcttatcct ttggtccttt tttctatctg ccataaagat tcaagcgaag taatggaaga
    13801 aatcctttcc ggcagaatta acctggggct tgtcggagca aagtatagct cagatgccct
    13861 tgagtatatt gatttttatg atgacagaat ggtacttatt gcttcgcctc aaaaaaaatt
    13921 tgattcggat tctgtcagta tcaggagttt aatcggagaa aacatactgc tcagggaaga
    13981 aggttccggc acaaggcttc ttcttgaaaa tgccttgaaa gaaaaggaac tggatatgtc
    14041 catgtttaaa tctcagatta ttaatgacag cctggaagca atcaaaaaaa tggtggaatt
    14101 ggatgtcggt atcagtcttg tatccgatat tgcagtaaag cgtgaagttg agtccggtca
    14161 actaaagcaa tacgaaatat ccgacctgga gcttaagaga agctttagcc tggtttattg
    14221 caataacaga tgcctttcac ctattgagga gaaatttaag gattttatca gcaattggaa
    14281 aagataagta tatatgtctg gtgcctggca attcattttg ccgggcactt attttttttg
    14341 cagcgatttt tgtattgaca acaggattag atagagatat tatgaaatag aacagtgttc
    14401 taaaaaagaa caaaggtaaa taattgaacg aagctaaacg gaggatgaac ttatggctat
    14461 cagtgaaaga agagaacgtg aaatagaaga aatgaaggaa ctcatactgt ctacggcagc
    14521 tatgatagca aattcagaag gatatgataa gctgtctatc agaaaaatag ctaacaaaat
    14581 tgaatactcg ccatctatta tttaccacta ctttgcggat aaggatgaaa tcataagcca
    14641 tttgatgcaa agaggttata aaaaaataat atcggcaata gcctctgcaa atatgataag
    14701 tgattctccg gaagagcaat taaaaaaaat ggctaggtat tatatagacg ccgcactgaa
    14761 aatgcctgaa gagtatatgg ctgcgcaatt aagcaaatct ccaaaaacta taaagcagac
    14821 ttcatttcta tatgaggggg cttctgatga aaagtccgtc ataggtgcat tatgcaaatg
    14881 cataaaagaa attaacaagg atattgattt ggaggacagc aaatgcgaac ttatttcgca
    14941 gttaattatt gcatctgcca tgggccttgt tataaagctt attgctgaga aagagacggg
    15001 agaggagcaa aggaacagat tggttgaatg ctttgcaaac gaggttgtgc ccaaaatagc
    15061 ccgtgtgtga ttttacaata ctgttgttat gttagagaat ggttctgagg tggaggattg
    15121 tatatgaaaa agaggttatt taaccggact gcctggtgaa gacggtatat ttacaaaaga
    15181 agatattgct gatctgccgc tgcctgttca gaaatatttc agatattgcg gccatattgg
    15241 cacatttcgc attacgatga ttgcgacctt gtttatttcg acggaaaaga cattgaaatt
    15301 gaattttatt acgaataaat taactgaatc aaaatatcag gcgttaattg gcgccattac
    15361 ttttctgtat tctttatgcc ggttgacctt gaagagatgc aatagtccag gaagcttttg
    15421 agagcaggag tatgctttat tctattattc cacagaagac tgatagtcct tctcactgtt
    15481 ctcccctcaa agggtatggc agttacctta tagccatcga gagaaggaat tttgggcata
    15541 atcgctattc ccagctgtgc tcctacaaaa gaggccattg agttgcactc gtcgacctca
    15601 aaaaagatat tcggcacgat attgaatttc ttaaataagg aatcggtctg aaaatggaga
    15661 tcggtttctt tttttatcat aataaatttt tcatttttga aatcatctat tgtgactgag
    15721 gtatgacttg ccaaaggatg atctttatat acaaccagaa atagctcctg ttcaaataga
    15781 gggatgaatt cgatgcactc attcacaatt tcagtagatg gagccatgac aacatcaagg
    15841 gtgccttcta tcagcttctt gatcagtaaa tttgtcatgt taacctgaaa gctgaaactt
    15901 atattgcggt tgccctgaca ggcgtagaaa tcatctatta aatgcggaat ttcatcaaag
    15961 ctgacactgt aaatgtatcc cagattaatg ttccccccgt aagggctatt cattctgtta
    16021 agctgagctt ctccctggat aagtatgttg agggcactct cagcataggg gagaaaggct
    16081 tctccgtatt ctgtcaatga aacttttata ccttcctttt taaacagcgg tacacccaat
    16141 ctcttgcata actgggatat ggcataactg aggctgggct gagatatatt cagttgttcg
    16201 gctgccttgg tatagtgcag ggtatgagct gtttcaacaa agtatcttaa ttgttccaag
    16261 gtcatggtat cgcctcctta gaagtttaaa ttttcattgc tgcaaaagtc aaatactcat
    16321 tgtggagata taaacaagtc atagagaact tctattaaat tatacaaaat gggtattgga
    16381 cagtcaaggg aaaatgctat aaaattataa taatataatg aatattcaga aaatcatatg
    16441 aaaaaattgc aaatattaga gaaaaaagga taaaattgaa aatgtacaaa aaggtcattg
    16501 agcatatcga agaagttatt tttcacaata taaaaaaatc atttaaaaag caaaccctag
    16561 aaaaccggtc aatttgcagg tgagtgaaga gagattaaat ttcaaacgca aacaagtcat
    16621 agagaaattt tattatatta tacaaaatga gtattggact gttgagagaa attaatatag
    16681 acttttctta acatataaaa cattcttata actataagga gggaagaagt atgaatcttc
    16741 cgtcaaaaat aactatttgt gaagttggcc tccgggatgg cttgcagaat gaaaaaaaga
    16801 ttttgcagac tcaggaaaag ctggaattgg ccaatggcat tatagctgcc ggcttcaagg
    16861 taattgagtt aggttctttc atgaatccga aggcagtacc tcaaatggca gataccgatg
    16921 agcttttctc aactttgaat cagattgatg gaatagagct gcgtgcacta attgccaata
    16981 aaaaaggtgt agaacgcgcc attaattgcg ggtgccgcaa ggtcaagctt aatgtgtcgg
    17041 caagcctcgg gcataatctg gcaaatctga acaggacacc tgaggaaagc gttgccggct
    17101 ttgaagaaag tgttgccctt gccgagcagc atggaattaa ggtatccgga tcaatttcca
    17161 tgccatttgg ctctccctgg gagggaaaca tatcgctgga atctgtcaag agtatagttg
    17221 aagcctacct gaatgtcgga atagacgaaa tatccctatc ggacgcttcc ggtatggctg
    17281 tgcccaatca agtatattca atgtgcatca atatgcagga atgctatccc caggtgaaat
    17341 ggtggcttca tttccataat acaagaggaa tggccatggc aaatatactg gctgcgatgc
    17401 aggccggaat aacacaattc gactcctcct tcgcagggct gggaggttgt ccttttgtcc
    17461 ccggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaacac aattcgactc
    17581 ctccttcgca gggctgggag gttgtccttt tgtccccggg gcagcgggga atgtatcctc
    17641 tgaagatgtg gtacatatga tggatgagat gggaatagaa acaggaatag atattgtcag
    17701 attaatccgg ctgtccggaa aggtacgtca aatgataggc catgacactg attcatatat
    17761 acttaaggca ggccgctcga aagatttaat caaagaacag taaaattcaa agaaatggtt
    17821 tccggcatgt gaagcgtgga tatctgtatt tccatagcag gagacatata acatattcag
    17881 aacgaacact aatgtttagg aggaatgatt atgagcagga tgagttcaat ggaagaagta
    17941 tattcaaagc ttaaccagga ccagatagat gaaatcgagg aaaagtttgc aagagcagat
    18001 gcggcgcaga agatatttga aaaatggagc caggaagaag ttgaccgcac tataagagct
    18061 atagcatgga aagttgcaaa tactcagacc tttaaagaac tggtcaagat gagcatagag
    18121 gagagcggga tgggagaccc cgtaagccgc gagaataaga gatttaaaat cagaggtgtg
    18181 ctgagagatg cgttgcgtca gaagagcgta ggtataattg aggaaatacc ggaaaaaggg
    18241 atagtcaagt atggcaagcc ggtaggagtc attgcatgtg ttgttccggc taccaacccg
    18301 gacttgacac cggcaggcaa tgccatatat gcccttaaag cccggaacgc cataattttc
    18361 tcaccacatc caagagcagt aaaaaccgga ggccgaacaa ttgagcttat gcgtgaaggt
    18421 ttgaggcagg taggcgcacc ggaagatctg attcagatcc ttggagttgg aaaagctaaa
    18481 ataaacaaag ccatgtctga ggcgcttaat acgaaatgcg atttggtaat tgccacagga
    18541 ggacagggtg tagtaagaag agcatatcat tcgggcactc cggcttttgg tgtaggggcc
    18601 ggcaatgcga ccatgatttg ggatgagaca gctaaggatg agcttcacca ggcagcgtac
    18661 aatacaatgc tttcaaagac atcagatttc ggatccggat gctcagctga cggaaatata
    18721 gttatacatg agagcatcta tgatgatgca atagaggaac tggtcaatgt gggaggctac
    18781 ctgcttagcg aggaacagca gctggctgtc aagaagatta tgtgggatga ggaatgtcat
    18841 cgtcttcctg attcagtagc ccaatcgcct caaagaatgg ctgagcttgc cgggtttaca
    18901 ataccggagg atcgtaaatt cctcatagcc ataggcacag gatcatacac aacaagctat
    18961 gaggatgtat ggtgccgcga aaagctgaca actttgctgg ctgtacacaa atatgagggc
    19021 gagtttcaga atgcaataga cataataatg gctatccatg aggtaggcgg aaaaggccac
    19081 agtgtaggta tatactcata tgacgaagag catatacaca ggctggctct tgaagcccgt
    19141 gtaggccgta taatggtgag acagccgcaa tcaaaatcca atgcaggctc ctttaccaac
    19201 ggaatgccta tgacctcaag cattggatgc ggaacctggg gagggaatgc aacatccgaa
    19261 aacgtaaatt tacgccacta tatgaatgtt acatggctat caaaagctat tcctgaagat
    19321 aagcccagtg atgaagaatt attcggtgaa ttctacgagc ctgagagaga aggaagggta
    19381 tagaaggggg tattaccttg aaaaaattga tttccgaaca aatggtgaat tacctgcagc
    19441 gccgcggggt cgagtatgta ttcggacttt gcgggcacac ggtaattgcc ttcctggatg
    19501 cactttccag gagcagcagt gtgaaatatg tttcctttag aaatgaacag cttgcagcta
    19561 tggcagcaga cgggtatgcc cgaatcaaga aaaaggccgg agtggtaatg tgccacttag
    19621 gccccggtat tgcaaatacc gtaacagggg tggccaatgc atcatttgat tccataccca
    19681 tggttgttat agcaggagat gttccaagat attatttcgg aaagcatcct catcaggaag
    19741 ttaatctgca tgctgacgct gctcaatatg aaatcctgaa acccatagta aagagagcct
    19801 ggagagttga tgatcctgag gctatgcctg aaatattgga taaagctttt cgcatggctg
    19861 aaagcggaag gcccgggccg gttctcatat ctgtgccaat ggatgttttc tccgaagagg
    19921 ttgaagagga tcttttcgca cgtacttacc ttgatagaca ccagactatc aaacctgcat
    19981 tggcacctaa agctgcaaaa gctatagcac aaaagctggt ggaagccaag aatccggtta
    20041 ttcacgcagg cggcggtata cttctggcag gagcgtcaaa agagcttcag gatttggtgg
    20101 agtttctgga tatcccggta tcccggacac tgatggggca gggctgtatg tccgatactc
    20161 atccattgat gcttggacag accggattct ggggccttga atttacacat aagtttacag
    20221 ccaatgcaga tgttctgctt gggcttggaa cccgctttgc ggaggctgac agctccagtt
    20281 ggtacccggg cgtaaccttt gacatgagga agacagaatt tcttcaaatt gacatagatc
    20341 caacagaaat cggacgcaat tatccggtta gcattggtgc tgtagcagat ttgaaagaag
    20401 cacttgcaca gattctggaa gaagctgaaa agctgtgccc ggaaggcata aaaaggccgg
    20461 aactcaggga aaggatcaaa aagaatcgta cattattcag agagtcaaat attgcaatat
    20521 ctgaagacgg gcgcttccca atgacaccgc agcgtatact gaaggacgta aaggaaacga
    20581 taccggagaa tacaatcata tttaccgatg taggctggaa taagaatggc gtggctcagc
    20641 agtttgacat tactcttccg ggaaccattc accacagctc ggggcttgca accatgggat
    20701 tcggggcgtc ggcgctgctg ggcggcaagc ttgcggcacc tgacaaggtt gttatcacac
    20761 ttgtgggaga cgggggattc ggaatcaatc caagctcact tgccacagca gtagaacagg
    20821 gcattgccgt aatatggatt gttatgaaca actccgcatt cggtacaatt gccggtttgg
    20881 agtattccaa ctataagacc aaatttggaa cggtatttac cacaccggat gggaaaccat
    20941 actctccaaa ctgggcagag gttgcaaagg catacggggt ggatgcaatt cgtattgatt
    21001 cggctgaagc cttcaagccg gctttggagc aagcattggc cagcaatagg cctttcctgt
    21061 tggatgtgcc tatggagaat attggggtac ctacacccgg ctgctggaat atcaatgaca
    21121 tatatacacc taaagcaaat attgtaaacg gcaaaattat aaagaatgag aaaggtgaat
    21181 atgaagctcc gaaccattca ggttcacaca aataaacagt gtatttgaaa ggagagcaat
    21241 tatgccaaga aagttttccc ttgcttatct tacaataccc gggactggtc cggtggatca
    21301 gattcgcata gctgcagagg cgggatatga tttcgtcagc ctccgcccga taccgatgca
    21361 tctccctaac gaacctcttt tccggtttga tcaggataag aagctgttca atgagattaa
    21421 gcatgcgctt tcggaaaata atatcaggct gttggacatc gaactggcca gagtaagaga
    21481 ggacttgaag gttgaggatt atgaatctgc attttttgcc gcggcagagc ttggagggac
    21541 agatgtgctt tcaagcatat ggactaagga caaggcttat tattataggg aatttgcaaa
    21601 aatatgcgat cttgctgcaa agtactctct tcgtgtgaat ctggagttcg tgaccttcgc
    21661 aggggttaca tccctggttg aagcctcgga tgtactcaat acagtagaac gtcccaatgc
    21721 atacctcatg gtggatacgc ttcatgctca caggtcaagg gtatcggcag aagatattgc
    21781 taaggtggaa ccttcaaaat ttggatttat tcacctttgt gacggcccgg gtcccatacc
    21841 tgcattggat gatccgggca tgataggagt tgcaagagag ggaaggcttt atccggggga
    21901 tggagaaatc gatttaaaga gtatgtttct tgcaatgccg gcaaatccaa tatcaattga
    21961 attgccaaat tccaaggaga tggaaaagag aggagcattt gggcatgctg cccaatgcct
    22021 gataaaggca aagcagtatt ttgccgaaaa tggcatagaa tagataaagg tacagtggtc
    22081 tttgatatta tataatataa aaagggggga gaaaaatgcc ggttaatatt aataccaaga
    22141 tgataaccct gctgggaaaa cctctatctc agtcctatgc agccaggatg cagaatgcgg
    22201 cgtatagggc agccggaatc gacatgctgt atttttacac agaagttgaa aatgatcatt
    22261 tacccgatgt aatcaatgga atcagacaca tgaattttgc cggatttgcg gttaccaagc
    22321 caaacaaagt ggaagtcatg aaatatgttg atgaagtgga tcctctatgt gaaaaaatga
    22381 atgcaagcaa tactgtagta attaaaaacg gacgccttaa ggcatataac actgacggta
    22441 tcggtttctt aagatctctg aaggaagaag cacctgaaat agacatctct gaatctgtat
    22501 tcttctgcct cggagcagga ggtgccgcca gagctatatg cagtgtactt gcatataacg
    22561 gagcaaagaa gatatacatt gcaagcagaa ccttatcacg agtcaaggaa ctggttgaag
    22621 acatcaatga gaagtttgca cctgtggcgg aacaggtgga tatgcaggac acagagttgc
    22681 tgaagaaaag tatagcagca tcaaatgtta tcatgaacaa taccggattg ggcatgcctg
    22741 caagcctgga caaaactccg attcccaaag agtatctcag tcctgtgcag ttgtgttttg
    22801 atgcaaccta taatccgtca aagaccagat tccttacaga agctgaacaa gttggctgta
    22861 agataattaa tggcttgggc atgtcgcttt atcaaggtgc tgaacagatt gaactatgga
    22921 gcggagtaaa tgctcctata gaagcaatgc gccaggaact ccttgatata ctttcaggga
    22981 aagaggagaa atagtgtaag taacgtatgt attatactta cacaatggga aggggggcaa
    23041 caatatgaat ggacttaaat ggttgaacaa gcatttagaa gaagctattc tggtgtttct
    23101 gttagcaacc atatcatgtg taatgatggc gcaaatcatt gcaagaacct tttttaattc
    23161 catgacctgg cctgaagaat tttccagata ttgctacata tggacagtat ttctttctct
    23221 cggatataca atcaagaagg ggaatatgct gaaggtagga atagttatgg acttgcttcc
    23281 ccagaagctg cgaaagagaa tagagattat tgtaaacgtt ataatgttgg tattatttgt
    23341 aatattgctc aggtattcaa taatatatac cggcaaagtc aagagcagcg gacaattttc
    23401 accggctatg cacctgccga tgtggattat gtacatgtca acaattatcg gtttcgcatt
    23461 agctgcctta cgtatggtac aggaaatcat ttcaaatatc aggaacttca acaagaaagc
    23521 tgagacgact ttggaagcga caataaagga agcgaagcag gaagttcagg ccactggcat
    23581 agctgttaat gataatgacg gtttaatggg aggtgaggat taatggcact atggatattc
    23641 gttgtatttc tgtcagcact tgtatttgca gttccgatag ctgtaagtat ggtgatagga
    23701 gctatgacac cgcttttttt aggagggacc ggaagttcca tccagcagct gattgcaaac
    23761 agcttttccg gttcggatac gacaccaatc ctggcagtac ctctcttcat tcttggagga
    23821 gtacttatgg cagaaggcgg gatatcccga aggctcttca atttctttgc atattttgta
    23881 ggtaatttaa ccggcggatt gccatgcgct gttattctta cttgtctctt ttatggagct
    23941 atttcaggat caggccctgc cactactgca gcagtcggat cgatgacaat tccattcctg
    24001 gtggatatgg gctatgacaa gacttggtca gcgggcatgg ttgctactgc aggcggcctt
    24061 ggagttatca tacctccatc catacccttt gttctgtatt cactggcaac aggagtatca
    24121 accggagctc tcttcctggg tggagtcctt ccgggtatcc ttatcggact ctgtatgatg
    24181 gtttatgccg ttatctactg taaacgaaag ggagaaaaca aagagagaat aaatgcaaag
    24241 atgacagagc tgcgctcaaa aggtttcctc agtttactga aggagagctt ctgggctttg
    24301 ctgtcacctg taatcgtatt aggaggtatc tattcaggaa tagtaacacc gacagaagcg
    24361 gcatgcatct cggtatttta ctcactcttc gtatctctga taatctataa atcctttaca
    24421 attaaggaga tcattccgtt tcttgggaat gctgtaaaga cctatgcacc gctttgtttt
    24481 gtacttgcct ttgcaatagc cttcggacgg gtattggcct tagctaaggc tccaatattg
    24541 attgagaact tcatactgaa caacttctca acaagtatta cagcattgac gggtattgta
    24601 gtggttttct tgctgctggg aatggtaatg gatacagggc ctgcaatagt tatactgtca
    24661 cccattctgc ttcctgctgt tcaggccctg ggagtggatc cggtacattt cggcgttata
    24721 atggtatgct gcctgtcaat aggattagca actccaccct tcgggctcga ccttttcgtc
    24781 gccggaacac tgtccagttt acctccgatg aaagtggcaa tgaaagcaat gccctttata
    24841 atcgcatttg gcatagcgct gtttattatt acatatatac cctggtttag ccttgtattc
    24901 ctgtaaaaca ggtattcaag ttataaatga aaataagaaa agggggaaaa aaatgaaaag
    24961 gttatttatc ttattattgg tactggttat ggcattagta ccggcagcat gcgctaaaca
    25021 agcaccgccg gcagaacaac cggcagaaca accggcagca gaggagcctg cagaaggcga
    25081 aaacaaatac gcttctttgg aaccaataac ccttatcggt gctgataatg caggtgtcgg
    25141 cgccgcagca cagctttacg gtgagctggt taccaaaaag gtagcagaga ttaccgacgg
    25201 aaagctgaaa atcgattatt tcccgaacag ccagcttggt aatgaccagg agcttcaagc
    25261 tcagatgctt gcaggagata ttgactttgt aatagcacag acagcccaaa cggtgtcctt
    25321 cgtaccggaa gttgctatat ttgaccttcc aatggtgttt gcgaagtatg atgccaagac
    25381 tattgactat gcactcaaca agagtccttt tgccgcaaag attaatgaag cttataaagc
    25441 taagaacatg cgtattatgc actatctgca gggcggaact ttccgtgaga ccacttcaaa
    25501 caagaagatc agttctattg atgacttcaa aggtttgaag ataaggacaa tggaaaatca
    25561 gaaccacatg gctttctggc aagcacttgg agcagctccg acaccgcttc cgtggcctga
    25621 ggtttatgta tcattgcagc aaggccttgt acatgcacag gaaaatgcaa ctgatacttg
    25681 cgtcggtgcg aacctgcaag aggttcagga ctacctgatc atgacccacc acatacttta
    25741 ttgcaaccaa ttcctaatta attcagagaa gtttgacagt ctggatccgt tatatcaggc
    25801 tgctctccag caggcagtcg atgaggcagc tgttgagatt gaagcaaagc ttacaaccat
    25861 agataaagat aacaagcaga agctgctaga cggcggaatg gaactggttg aatttgaagc
    25921 cagcttcata gatcaggttc ttgagaaggc taaaggcgta tatgattcta taggaaaagc
    25981 catcgggcag gattatgttg actctctgct ggatgcactt gagaacgcaa aataacccgt
    26041 aattgaaaat cgaaatgcag ctttttcgaa atcaaacagg gtagaaaaat ttaaacagtt
    26101 aaaacagagg tcgagagaat cgacctctgt tttgcatgct gcaaggctaa gaaaatgagg
    26161 ttcctgcact caataacaca tttctgcaag tgctgctgta atatcccagt attccaataa
    26221 aaaccagtaa attcaggcag gaaaaaatgc agtcaggaaa gaaaatatga tatagcattt
    26281 taaataataa tagtcggagg tttttatgtc aaatacacca aagcaaatta aagctataaa
    26341 cgttttttca aaaggaacta tcgctgctat tataattata acagttgtcg gattgagcat
    26401 attgggagtt tttcgcttta ccaggccgat tccggcagca ggcacctcaa aaacttcaga
    26461 aaacattgtt ttacccggtg agctgtcggt tacctttcct gaacaagggc aagccgctgt
    26521 tggaactgat acttttggtg ttattgccgc ttcgccgaat caggagtcca ttcctattgc
    26581 cagtgtggca aaaataatga ctgcatattt ggttctgaaa gctcacccac tgcagcccgg
    26641 tgaggaaggg ccaagcttga cgataactga tgaggatgtt gccggttata agtatgcggt
    26701 tcaaaacaat cattcttatt tacccgttgc tgcagggacg gctcttacgg aaagacagct
    26761 tctgcaaggt ttgatgctgc cctctgggaa caatatagcc gatacaatag gacgatggat
    26821 tgcgggaacc gatgaagcat ttattgataa aatgaatgaa acagcaaagg ctctggggat
    26881 gaccaataca cactatgccg acgccagcgg ggcagatgat gctacagtga gcaatgctgc
    26941 cgatcaaata ataatggcgc aggcagctat gcaggatccg gcctttcggg aaatagtcgc
    27001 aatgccccag gctgtccttc ctgttgccgg aaaagtatat aatgtaaatg ccatgttagg
    27061 caaacacggc attgtgggaa tcaaaaccgg ttccggtgtt atagcaggcg ggaacttcgt
    27121 atcagccgca ccgatagaaa acggaagtga aaagcattat attatagctg ccgtactggg
    27181 cagcaggaag ccaaatgaaa acctgaaaag cgcctttgat gcaaacgccc agattctgaa
    27241 ccaggttcgg ccccagttca aaacatttac aattgagcct ccggaaaatg gttttggcaa
    27301 aattatcact ccatggcata ccgaaagcga attgacggta aacgagccga tacaggtctt
    27361 tggctatccg ggtatgaaga tggattattc aatagaacct ttggaattaa agttacctgc
    27421 ccttccgggc actgatgtcg caactcttac aatccaaaca gggaaaacca ctcaaaccta
    27481 tatgatgcaa aacaaagtgc aaattgaaaa gccgggattc ttatggagat taataaggaa
    27541 ttaattttag gaatggtaga gcactatgtg gtagttacag caaagggact gtttcatatt
    27601 gtgatatgat acagtctttt ctaaatccac ttattatccc atagatactg ttaaaaagtg
    27661 tttaaaaagt gtttaattgt aatctgatta aataaaaagt taatttattt taaacatata
    27721 attaaaaata aaaacaacac ctctgatata taattgacat aaaattagtg aggaggagat
    27781 atttttggaa aaagatttta agagaccgtc ttttcttgtt tcattactat cggtagtatt
    27841 tcttttcata atgattattg cacaattaat agcagtcggc agcccggata ttcatttgac
    27901 tttagtattt gccatcacat taacggttct tatgcttatg actaccggaa caaagtggga
    27961 aaaagtgcag gaaggagtta tgcacgggtg tgaaatagct actattccta tgttaatact
    28021 tatgcttata ggtatgctaa tcccgacgct tatagcatcc ggaacaatac ccacactgat
    28081 atactatggg ttgaaaatta ttaatccgtc tgtgttcctg ttaaccgcaa ctctaatttg
    28141 ttccgttgct tcaatttgca cagggagcag ttataccacc ggaggaacct ttggtgttgc
    28201 ctttatggga ataagcctcg ggcttggaat accccctgca ataacagccg gtgctgtaat
    28261 ttccggagct gttatcggag ataagatatc tcccttgtct gattctacaa atttagctgc
    28321 aggggttaca gaaacagacc tttttgccca tgtaaaaagc atgttttata ccacagttcc
    28381 ggcattcgta gtttcattaa tactgtatgg cataatcggt gcgagatata gtgcgtcttc
    28441 cattgacaca accgtagtag accaaatact tgccgggatt tcacaaaata tcaatataac
    28501 cccaatgtat gcgttgatca gtttaattcc gcttatagct attgttgtga tggcggttaa
    28561 gaaagtaaat gctcttgcag ctatggtcat tgcttcaata atagctatgg tattagcaat
    28621 gattaatcaa ggttatagca ttattgaaat gatggcattt atgaattatg gcttcagtat
    28681 tgacacaggg gttgcggaag tgaacaagct tctaaacaga ggcggaattc agtctatgat
    28741 gtggactgtt tctttggggt acttagggct ttcctacgga ggaattctag aaaaaacagg
    28801 tataatggaa gtattgttaa acaagatgtc tgcattagta aagaatgcaa gaaaccttat
    28861 tattactcat gtctttgcag cactcattgt caacttaatt tcagccagcc aatatgtatc
    28921 aattattatt cctgggagaa tgcttttgcc ggcttacaga agacttggta taagaagcga
    28981 tgttgcttcc agaacttgtg aagactcggg gactgtttta tcgcctttgg taccctgggg
    29041 gctatgtggt gtgttcttca caggaacttt gggtgttaat acattggaat atatgccttt
    29101 cgcattttta tgttatctga cacccattgt cgcgattata tacgcaatta caggaaaatt
    29161 tatttggtat gaaaaggatg gaatgataaa gaatgatgta aaaaaggctg cggaaattag
    29221 tggataatga aaatcggctt tggaaaggga ataaagatgt atgaaatcga aaaaggccaa
    29281 ttaacagaat tattaacaga tttggtttct attgaaagcc cgtatttaga agaagatgag
    29341 ataatggagt atgtatacag ttggttccgg aagaatggta taaaggcctt tgttcatcat
    29401 tataatgaag caaaagttac aggctttaag ggaaagaata ttgttgcaaa tattgaaggt
    29461 aataagaatg gaccggtaat atgtttgaat ggtcatttag ataccgttaa gttgtgtaac
    29521 ggatggactc ataaccccaa aggggaaata aaagatgata ggttctatgg agtaggagct
    29581 ttagatatga aatccggatg cgcagcaaca atgattgcag taacagcttt tgctgaaaat
    29641 cacaaggatt ttagaggtaa gataattgcc acgtatgttt ccgtagaaga gggaccctat
    29701 gggatgggaa caaacgcttt gattgatgaa ggatatttaa aggatgttga tttttcaatc
    29761 agcacggaac caagtgccgg gttttcaggc aacaaatttc ccgatgtttg tttaggtgct
    29821 agaggtggat acggtttgga tatagaattc ttcggtatat cttctcatgc agcgactcct
    29881 gaactgggag taagcgcggc ggaggacgct tcaacggtaa tcagcgaatt aaaaaatatt
    29941 gagtacatta aagatcccca tctcggaaca ggcaatgctt gtgttatagg tattgagtgc
    30001 gacggaggtg catgcagtgt tcctgattat gctaaagtga agctgttttg gcacattgtt
    30061 ccgggagaaa atgaagagac aataaccgga gaaattgaaa aagcaataag aagagctagt
    30121 attaaatgca aatacaaaat taattttaga aagtcacctt cggaagggtc taaaggtttt
    30181 atgccttata tagtttcaaa agaagatccc atggttaaag aatttttgga aactgttgaa
    30241 gaagtatgta aaaccaagcc tgagatttct tatttccaaa gcatcggaga ttttaattat
    30301 ttaggttcaa gattaggcgt tccggttgtt atcttcggcg cttcaggaaa aaactttcac
    30361 gggaaagatg aatatgtaga attagattcc gtagtaaaaa ctgctgaaac tgtctataga
    30421 tttcttgaaa ggatgctgtg tttataggtc aataattgat aatgcgcacg ctagtgcgca
    30481 ttattcttct actagcttta gcccttctat tttaattcgt ttaagatatt ttagtacatt
    30541 aagggaggta tcatccatat cggtattgtt cagaattatg atcgtaagtt cctgtttact
    30601 gcagataatc cttaataatt ttacaaaagc atttattatg catacgctca ttgtgtcacg
    30661 cccataatcc tttatatgtt caaacttatc gtcacaataa tacagaatgt cacattgtat
    30721 atatgctaga tccagtaatt ctttttcgct caattgattc aaataatcag tacctacagc
    30781 ttcgattatt ttgccaacta catcggagat ccaaaaataa tctacgtttt taatgcaatc
    30841 ggaaataatc tctataaccg aaacattctc ttgtttacta atgtctttca tgaatgtatc
    30901 catatttgct ttagaactat tattatcaat agaatccgat aatacggacc ttatttcttc
    30961 atactttttt ctaagttgat ttgaaggaga tatcccaagg ctgcttgtca gcaatttgct
    31021 gaaattattg taatatgtta ttgcggcaac cggttttctg cactttacat aaatatcaag
    31081 tatttttagc accatttcct catcataggg ttcaatttca aggatctcat ttattacttc
    31141 aagacagtta tcaaagctct cctctttttc atagtagtcc accaaactct tcagtatttt
    31201 aacttttcgc tgttcatatt tcattctttc aaatatgata aggtcgttga gttcatcaca
    31261 attcttaaaa taacagcctt ccagcaaatc tcccctgaat aattgtttga gctttagaat
    31321 actctccaaa gagtcatttt ggctgtgttt aaagctcatg atttcaaagg tatcacattc
    31381 aaattcatat tctttattta tggaacagca atcactatct acatgcaaaa atttatgccc
    31441 cttatgatca gttcctatat ttttttttat aagccacaaa ttatatctta aattatacct
    31501 tgctgcgtcc gtactgctgt ctggccagag ataaccttca agcttctctc tgctcatata
    31561 tcttttttca tttaacacga gaaggcagat taaaacaaca gccttactgc ctaactgttc
    31621 ttccagtttt ttaccgttat attcaactct tggcttaccc aaaaaattaa tactcagcat
    31681 aatatttcac caatccctat caaaggttac tatataatgt ttcacctgat tttatagtta
    31741 cgcagactct tgctttttct atttggtgtg acggaactaa aagtatatct ctatccaata
    31801 ttataatatc tgctgttttc cctacactta aagtgccctt gtttttctct tgaaaaatgc
    31861 cgtatgcgcc attgcatgta aacattttca atgcttcata aatatcaact ctatgcttta
    31921 caaccgggtg atttactgcc gagtgaatac ccagtaaggg ccgtggctcc gtaacatcac
    31981 tatcggagcc tccgcatatt attacacctt tttcaatgat ttctttaaat gggtttgtaa
    32041 gaatatattt gtctcccagc ctttgttcat acatctttcc cggacctccc caataggttt
    32101 cataggaagg ttgcatagaa agaaggattc ctaattcttt agctctttgt atttgttttt
    32161 catttatcaa ttcagcgtgt tccaaacgat ggcgaagtcc aataattcca gtctgataaa
    32221 atgcgttttc atgggcattt atagccgttt ctatagccct gtcaccgatt gtgtataagg
    32281 caagctgaag gttgttttta taacagttca aaacaaaatc gttgatttcc tgctgagtta
    32341 gaaggaggct tcccatggta cccggacagt cagcgtattg aaaagaaagt gctgcggttc
    32401 tggcgccaaa agtgccatcg acataaaggc atcctccaag tcgctttaat cccatggctt
    32461 taattttttc aatgtccata gtttggtaaa atagttcaat atctatagga aactctttgc
    32521 catgctcata taggaaatct gcatctttat cactgtacat aaatccgcct tccatggcgt
    32581 taatggtagt tatgccattc atgagaagag ttcccattat tccctcaact gcatctttcc
    32641 tgatgttatc tgaaatattt tttagtatat ttgtacgcaa aatagcattg gcttgtttcc
    32701 taaaaatacc tgtgggcatt tgttttctat ccagttcaat accttcagct gtaaaaggta
    32761 ttttaaaata tagcatagca taggtgttta atatgcttac ctgatattcc atggaattaa
    32821 gtacaacagg aacattactg caatacttgt caaggagcgt tctcccgggg aatctcccct
    32881 ctgtaaactg ttcttccgat atacggattc ccctgatata gcctccggta aaagaggatg
    32941 ctgcttctct tagctgttcc cccaactctt cataggtttt tgcatagctt aaatctacac
    33001 ttatagagtc aagtgcagtt tgaatcacat gaaaatgact gtcgataaat ccgggaagca
    33061 cagtatttcc ccctgcgtca aacacaatcg tatccccgtc aataagagac tcatattctg
    33121 tgttatcccc catagcaacg attttgtttt gttcaacagc aatccatttc ttttcttctt
    33181 ttccttccat agtcatacat cttccgtttt taatcagaat acttgcatgc atagggtctc
    33241 ctccaagttt gtaagtaatt tatccattca tttttattgt atcataaatt tccattgtaa
    33301 acatttacaa gttgcctgct tcatcaagga agcaaaagaa actttacaga ggagtaagta
    33361 cgaatatgca gatgtccggt gagttatggt gccaggcacg acagatagac agttattgac
    33421 aggaataaat ccttatggta aaatatggat atagttactg ggaggatttt ttatggggag
    33481 accgtggaga gaatagttaa aggcgggata tatcatgtta ttgccagagg taataataaa
    33541 gagtatattt tcaaagaaaa cgtagataaa ggatatttta taaagcagat aaaagagaca
    33601 actgcaggaa tggggtataa agtgtttggg tttgtgttga tggataatca ctatcatata
    33661 attgtccaga catttgacaa aaagctgcag gatataatgc accagataaa taataaacac
    33721 agcaaatact tcaattataa atataaaagg gtagggcatg tctttcaagg taggtataag
    33781 gcaatactgg tgcaagatga aaagtatatt gtaggattat taaggtatgt acatcagaac
    33841 ccagtggaag ctcatatatg caatgacatg gataagcctg gaagattatt atctgttaag
    33901 gtcaggttca aggaaaagaa cattgacagc atataaattg gagtatataa gaaaggcatt
    33961 gaaactgaat tacacatgca aagagatagg tgagtatata agagcaacgt aaagtgcaat
    34021 caaagaattg atagccaagt acagtagata actgtctaac tgtcgtgcct ggcaccacat
    34081 gcatgtagca tccggctcaa ttatagttaa ttaggcgaat ggacagaaga atggacagaa
    34141 tagtaagaag gatagggtca gacaaataaa gtctgacttt ttttctggaa acggttgcgt
    34201 tttttattat aaagaattat aattaatata taatcattat tgataggtct ttggttattt
    34261 cactatattg aaacatattg tagaaaagct tttgaaaaca tataattata gtaataataa
    34321 ggaggataaa aaaatgagtg aaaacaaaac ccttgacaat ttaatggcgg catttgccgg
    34381 agaagcccag gctaacagaa agtatacggc atacgcaaag aaagctgaaa aagaagggaa
    34441 aatgaatgca gccaagctgt tcagagcggc ggctgacgcc gaaacccttc atgccttaaa
    34501 gcattttgaa gtggcgggaa aaatcggttc tacggcagaa aacctcaaag atggtgttgc
    34561 gggagagacc tatgagtata aggatatgta tcccgatttt gtgaaagtgg ctgaagctga
    34621 aggaaataaa gctgcgctca tgtcctttac ctttgcaatg aaagcggaag aggttcatgc
    34681 cggactttat caggatgcat tagaaaacat cgatgagact gaagaagttt tctattatct
    34741 ctgcccggtc tgcgggaata tcgaaaaatc caggcctgaa aaatgcagta tttgcggcgt
    34801 atcaggagag aagtttatta aatactaggt gccgggcgct gtgaggtaca tttaatacac
    34861 cttctgatga tattatatca gaaggtgtat tattccggca ggggcgaaat atatcacggg
    34921 gcagcgtgag gaaggatgat tttaacgcag agccctccgg attcattatt ttccgcccat
    34981 atcgaaccat tgcaggcttc aacaatttcc ctgcatatgg ataaacccag ccctgctacg
    35041 gagcggcttt tatccgaggt gtaaaaggga tcgaacattt tttgcagttc cccctctgaa
    35101 acacccgtgc cgttgtcctc aaccgaaaat attatgcagt tttcctgctt tgagcaaaat
    35161 actgcaatgg agggttctct aacactgaaa tgcttcaggc tgttgccgat aatgttcccg
    35221 aatacccttc tcatcttagc aatatctata aacagtattt tatcggggca gctgacactg
    35281 atggaaaaag aaactcctct ttccgccaat tcttcttcgt agtctgattt taagatggca
    35341 cagaactttt ctgcggatat cctctgctgc tttaacccgc tctgcatctg gaggttaagg
    35401 tactcgtcga attcctctat caaatccctg attgaaactg atttattata aattatatca
    35461 atataccttc catatctgtc tgcggtaaga ttgttctttt ttaaccgttc ggcataaccc
    35521 atgacagaag tgagaggtgt cttaatatca tgggaaatgg atgcaataat aaggttctgc
    35581 ttctgctttt ctttttcaat tgcttccgta agccttacga accggttttg cagcagacct
    35641 atctcatctt tgcgtgccac attctgagga tggattcctt ccttatacct ttccatgctt
    35701 ttttgcaggg ctataatggg cttgacataa ttaaaataaa taatagcagc aataaataat
    35761 aaaatagagc atatgatgaa tatttccgct ataaacaaat tccaaaccac ataatatgag
    35821 gaaatctttt tcagggatac cggctgagta accttcagca aatagacttt attatcatgg
    35881 tgaaatatac cggaggaatt aatctctatg ctcacgccgc cgtcttttcc cccatggaat
    35941 accgtacggc cggactcatc cgcaacctga atattcagat tttctgtctg agaaataatt
    36001 tgaagcgcct gtgagaaatc ctgactgctt tcgattcttt ttataatccg gtcagtttca
    36061 gtctgcagct gctcccgcat attgctgttg tagccggcaa tttcccggga aagataaaag
    36121 ttataatacc cgtaaagcag cattatattg ataagaaaag cagcaagaac aaggagggga
    36181 agcttgaatc taatactcat tttctttctc ctggtgtttt attaatttat atcctacgcc
    36241 ccatacggtt ttgatgtacc tgttatcctt gtcgatttta tcccgtaagt tcttaatatt
    36301 caccgctaca gtaccaatat ccgtgaaatc agcaccccag atcgcactga aaatctgttc
    36361 ctttgagacg acctttcctg cattaataaa aagataatgc agcagttgaa attccctcat
    36421 ggataattcc accgccctgc cgtttacggt gacttcatag ctttccgggt ttattgaaat
    36481 gccgcctata gtcatgatac tgttgttctt ttggaggctg cgcttttcgc ggcgcaaatg
    36541 agcctttacc ctggcgacca attcctccac aacaaagggc tttgatatat aatcgtccgc
    36601 acccatttca aagccaagca tcgcatcata ggtcctgttt ctggccgtca cgaaaagtat
    36661 ggggcaggat actgtttttc tgatttccct gcatatttcc agtccgtcta tgccgggcat
    36721 cataatgtcc agcattatca atgagaagtc ctgaatactc ttcagtaaag tgaaagcatg
    36781 ttctccgtca aatgccagaa tgcactggta tccttcatcg gtaagggcat cggatatcag
    36841 tgatgcgatt tccctgtcat catcaacaat caatattttt tccatgattt cactccttcc
    36901 tgttagcata aacatccacg ctgtaacccg gtttcagtac catcccggga tcctccggct
    36961 taatctgtac ctttataatc cgtttgccgt ccttttccac agcttcattt gaaatctgga
    37021 taacggtccc cggtatcgag gtgctgctgt ccgacgccgg tacaatttca acagaatcat
    37081 caagagcgat attcctgata aattcctctt ccacctcagc aatgactatt atagaatccg
    37141 catcaatcag ctgaagaacc cttgtgggat tattctgtac ccccagcctt gtgccgttta
    37201 caacttcaat attccgtaca atgccgttcg ggacacatga tatgacttga tcagagtcca
    37261 ggtagtcctt aatgcttttg cttttcataa tctccaggtc aatacgggat actgccacac
    37321 cgctttcctg ccttgccgtg tttgagatgt ttgagtcttt tatttccttc agctgggcac
    37381 tcttggattt taatagtatg ctcaactggt caagctcggt tcttaaagcg ctttttgttt
    37441 tttcaatgcc ggtctcaata tccttcaggg ctttctcctt ttcagccaat atatccatgt
    37501 accggtccaa tgtgttcccg gagactgctc ccgcatcata taatgcctga ttgctgctta
    37561 catccctttt tgcattttcc gcttcttttt tcgccaggtt cagtgaggtt ttcagtattt
    37621 taaggtctgc attggtttcc ttggccaatt cctctgtttt ttcaagtata tcctcttgta
    37681 tctgttggat atccgcttcg agagccccgg tatccttcct gatgctttga agccctgcct
    37741 gtcctgcttc cagttgatgc tgaagctttt ccacggttcc cagatattcc gacatgtcca
    37801 gtgtaaccag tacctgatcc agggatacat ggtctccctc tttaacttta atatccgtta
    37861 ctatggcagg aaagtcaata cttatatcat aaatcctttc atactttact tctccccaga
    37921 aatccagcct gttatcctgt tccgatgaag aagataccgt ttcaaagctc tgtgccctat
    37981 ccgcccccgc ggcattaaag cttattatca cagccgctgt aagcatgata aggataccga
    38041 ttcctatcag aatacttttt ttgtgcctta gaaacatttt tttcacaata ttacctccaa
    38101 ctcagctatt ccacgctttt taacgactca atcatatcaa tgcttttcat ttttacggct
    38161 gcgatgctgt tgataaacaa agcaaatact gctgttatgg ccactgaaaa aaccacggtg
    38221 gcgaaggata cattgccgat caaatccatc tggtcattca ggccctttgc aacaatttca
    38281 gccagcacct tgcccacagg catgcccaga atcagccctg ctattactga aagggtgttt
    38341 tcagtgagta caagggaacg gatttccttc tggtaaaaac ccaggacctt caaagttgta
    38401 agatccctta tcctctcgga aaaattcaat atgctgctgt tataaagaac aacaaaggcc
    38461 aggattcctc ccataataat aagcattacc ccggctatat aaacaaccct tgtgctggat
    38521 ttgacatcgg caatctgatc ggctctgctt acatattcct ccgcataatc tcccctgaga
    38581 aagctccaat ccggcccgtt gtcccatttt acaagtattg cggtgggctt aaaaatttct
    38641 ccgattgatt catagtaggt atcggacata tacattccct ggccggtggc cagatacaca
    38701 atctgtttta caggcactct tatatagcct ttgtttgtcc gcttcagttc tattatatct
    38761 cctgccttga ttttcagggt gtctgcaagc tttctcgtaa tggcggcccc ttcatcaggc
    38821 agcaggacag gattgccttc gacatccttc aggcggataa gagggctttt ctgcggagag
    38881 accgtcagca attccaattg cctttctcca ttgggacaga tgatttccgc tgctgtttcc
    38941 cttatctgct gaaccgttcc ctccagcccc atgttatgta taaaacggga atccgcttta
    39001 ctgtccagga ggattttatg gtcgtaagtg tatgtaacat tgtaagtatg ccgggcaatt
    39061 ccattaatca tattaattat ggtaaaggct gctacaatca gccccgtgca gcctgtgatt
    39121 cctagtatgc tcattattag gcgtattttg tttctggatg tattccgggc aatcagcctg
    39181 cggctgaagc gcatcctgct ccaaaggccg gacaggtatt ccaggaatat atggcttcct
    39241 tccttggggg gcttaattct cagaagcacg gaaggggaat ctcccagcag cctgaggcag
    39301 gaatacaggg aaatgcctcc ggtgcaaaga agtatcaaaa ccaggcttat aataaaatta
    39361 ctgtggttta ttgaaatttt gtaatcggta aagttcagcc tgagccaggg gataagcatc
    39421 ttgccgaaga taacggggcc tgaaaggagg cctaaaaaag ccccaagcag ccccatatat
    39481 accccatagg aggtataatg ccacaggatg cttttctttc cgtaccccag agcttttaag
    39541 atacctatct ggccccgttg gttttccacc agcctcatca tggtgctttg ggttattaag
    39601 gcagtcacaa taaaaaacat cagggagaat accgcagcaa ggagcttgaa ctgctggata
    39661 ttggcattga tattgctgac gcttctgtgg tcctctctgg cgacaatacc tgccagatca
    39721 tctcccacgg cattaaaaat atgggttttt acctttgtta tatcggcacc gggagacagc
    39781 tttacggata tctggttata tattttccga ccgtatgcat tctttagcat gtcttcatta
    39841 ataataatga agccgtattt tttcggatca gggaatgtgt cagtggagcc tttcaccgaa
    39901 taaatatgtt cactgcttaa agccaggccc tggataggga agcgtatcca cttattattc
    39961 agtttaatcg agatatcatc acctatatcc aatccgtgca ttcttgcaaa aacttcgtcc
    40021 aggattgccc cgctgcggct tgaaagcttt ccgctaagaa gctggggccg gtccagtaca
    40081 cctttgccgg aaacagcata tacacgcagg gtagggctgt tttccagatc tgataaggca
    40141 tccagcacaa aacgcttttc cgccttttcc accccttcaa ttcttgaaat gccccaaagc
    40201 tccttttcgg tgggattcaa tacattgacc cataaatccg atatgttggc ggctgcgtac
    40261 atgctttccg aatgcagctc caccgttttc cagatgctgt cgataccggt gactatgcat
    40321 accgcaagag tggccattat aaggatagat aaaaactgga ctttcgactt ttgcatatct
    40381 ctgagggatt tgataaataa tgattgcatt accatattat ttcctctgca gcctttgggc
    40441 tgctgttgta tctgatatcg gaaatttttc cgtctttcaa atgcaagatc acatctgcca
    40501 tttccgaaat cagtacatta tgggtgacaa taatcaccgt ttttcccata tcgcgggccg
    40561 catcccgtat cagcttaagg actatccgcc ccgttgcgga atcaagggcc cccgtaggct
    40621 cgtcgcataa taatatctga gggttttttg ccagggccct tgcaatagat actctctgct
    40681 gctccccgcc tgatatctgg cttgggaaat tgtccatacg gtccttgagc cctaccttaa
    40741 tcagcatttc ttttgcgtcc aggggatctt tgcatatttc ctccgcaagc agcacatttt
    40801 ccagtaccgt taaattgggc accaggttat agaactgaaa aacaaagccg attttattcc
    40861 ttctgtaggt tgtaagttca tattcagcca ttccggatat ctccagattg tccgcaatga
    40921 ttcgtccttt tgtcggtgaa tccatgcctc caagcaaatt gagcactgtt gttttgcctg
    40981 caccgctttg ccctaatact acatttacag tacccttttc tatgccgaag ctgacgccgt
    41041 caaccgcctt gatgatattg ccgcccacat ggtattccct ttctacattt tcaaaagtaa
    41101 tgaagctcat aggttatctc cctttatgca tatatgctgc agcaaaactc agatgccatc
    41161 tgctgcctca cttattaaac aattatagtg gaaataaatt atatagtaag aggaaattta
    41221 ttaagaaata ttaagaatgg agaacattct tcaggtgcga ggttggaggt gcggggtacg
    41281 gggtacgaga ttttgagcgg ggctttgaag aaaatgctgt tcaaaaactg agcaataata
    41341 caaaaaagtg tacaaataat gaccagtttt ctttaaggtt ataggatatc cgttgagaat
    41401 actggttttt ctttttggca tgggaattgc tataaataga gggaaatata gccagattca
    41461 ggggtcaggc tgtattaaac gattattaaa tagtaaaagg gaggtagagt aatgcttaag
    41521 agaaaattat acgtaaacgg cgttgcaagg actgtgattg cagatcctga agcatctttg
    41581 gcacagtatt tgagaaagca gctgtgcctt acgggaacca agataggctg cggaaaaggc
    41641 gagtgcggag tatgtacggt aatcttaaac ggtaaggcgg caagatcctg tatcgtaaag
    41701 atgaaaaatg tgcctgatga gtcaaaagtt attacaattg aaggcatagg ttcccatgaa
    41761 aatcttcatc cgctgcagct ggcatggatg gttaacggcg gtgcacagtg cggattttgc
    41821 acaccgggat tcattgtgtc cgcaaaagct ttacttgacc agaatgtaaa tcccacaaga
    41881 gatgaagtaa gagaatggtt ccaaaaaaac agaaatgtat gcagatgtac aggatacatt
    41941 ccgttggtcg atgcagttat ggacgctgca agggttctta gaggcgaaat aaaaatggag
    42001 gatctgtggt gcaaactgga ggaaggcgcc agcatgctgg gttccaaaga gccgcgccct
    42061 tctgctctcg caaaggtgac aggaacatgg gattttggag cggatttagg cttaaagctg
    42121 cccgaaaaca cattacatat caaattggtt caggcgacgg tttcccatgc caatatactt
    42181 tccatagaca cctccgaagc agaaaaaatg cccggtgttt tcaaggtaat tacccataag
    42241 gatgtcccgg gaacaaacag gattaatggc ctggcatttc cgcagaataa gggagacgga
    42301 aaggaaaggc cgatacttaa tgacaagaag gtgttccaat tcggagatgc actcgctatg
    42361 gtgcttgcag atactccgac tcaggcagag gaagcagcta aaaaggtaaa ggttgagctg
    42421 gaagtattac ccgcttatat gagcgcacct gaagcaatgg ctgaggatgc tatagaaatc
    42481 catcccggta cgcccaacat ttattttgag acacacagaa taaaggggga ggataccaaa
    42541 cctttaatgg aaagcctgcc ctatgtcatt gaggatgact tttatgtagg aaggcagccc
    42601 cacctggttc tggagccgga tgtaggcttt gcttatctga atgaggaagg caagctgatt
    42661 gtccattcaa agagtattgg tttgcacctc catcatttga tgacggctga aggcatagga
    42721 attgaccctg ataagtatat acttgtacag aatcctgcag gagggacctt cggatataaa
    42781 ttcagcccca ccaatgaggc cttgctgggt gtggccgcac tggtatcaaa gaggccggta
    42841 ttccttgaat tcaatatgta ccagcagatt acttataccg gaaagagatc tcctttcttt
    42901 atccatctca agtatggagc agacaaagac ggtaagctga aggcaatgga agcggactgg
    42961 tcggttgacc acggcccata ttgcgagttt ggtgatttag ttaccatgag aggtacccaa
    43021 ttcatcggcg ccggttatga tataccgaac agcagaggag taggaagaac tgttgcaact
    43081 aaccacgcat ggggttccgc tttcagggct tacggttcac ctcaaagcct gtttgcatct
    43141 gaagtgctgg cggacatgat ggccgaaagg attggtatgg atcctctgga attcagatat
    43201 ttaaacgtat acagagaagg ctccactaca ccaacaggct gtgcaccgga tgttattgca
    43261 ttccctcagg ctatagatac tctcaggcca ctatataaag aggcaaaaga gagagccaaa
    43321 ggaaagaata agaatggcgg aaacatcaaa tacggcgtag gcatttccct taatatgtat
    43381 ggctgcggcc ttgacggcgc ggactcttcg gagatctggg tagagcttac ccctgaaggt
    43441 gttactgtag gtacttcatg ggaggaccat gggcagggag ccgacatggg gacactgaca
    43501 tatacttacg aagcattaag gccgttaggg ataaagcccg agaatataaa actggttatg
    43561 aacgatacgg agaaaactcc caatagcgga cctgccggcg gaagccgcca aaatgtagtt
    43621 acaggcaatg caatagtagc cggctgcaga aagctgctgg aagctatgaa aaagccggac
    43681 ggtacattca ggacctatga tgaaatggtc gcagagaagc tgcctctcag atacgaaggc
    43741 aaatggactg caccgaaaac cgcatgtgat gttgacacag gacagggaga tcccttctcc
    43801 acctacatgt acggcgtgct gatgagtgaa gtggcagtgg atacaactac cggcaaaact
    43861 caagtagaga aaatgactat tgtatcagac tgcggcacaa ttgtaaataa actggtattg
    43921 gaagggcaga tttacggcgg acttgtacaa gggataggcc tggcattgag cgaggatttt
    43981 gaagacctga aaaggcatac aactcttaca ggctgcggaa ttcctcaaat cttggatact
    44041 cccgacaata ttgagattat acatcaggaa acaccaaggc cttatggacc gtacggagct
    44101 tccggtgttg gagaaatgcc cctttctgcg ccccacgctt cgataatcaa tgcaatatat
    44161 gatgcctgcg gtgtacgtat aaagcatctg cctgcatatc ctgaaaaggt cttggctggc
    44221 ttgaaggagt taaataaata attttgattg taaaattcat gaaagccgga atatggaatt
    44281 gggattttat tcccaattcc tattttggca aaggaggtac aaacaatgga tctggataag
    44341 ctgcttgaaa ccagcgacag ttgtatacat gacgaaccac ccgcatgtac ctcatgctgt
    44401 cctgttcaca tggatgttat tgcttttgtc ggtgaaatgg agaaagggga ttttgacaaa
    44461 gcttataaag taatggaaaa acgggttcct tttgcaagaa taataggaag catatgtgac
    44521 catccttgtg aaaaggtttg tgtcagatcg gattccggag gaacggtcaa aatatcagaa
    44581 ttggagaaag cagctgttag ttatggcttt tctccgccaa agaataccat tcccctgtct
    44641 aaaagcaagg ggagagtggc agttatcggc ggaggaatca gcggcatgac ggcagccttt
    44701 gagcttgaca aaaagggtta tcaggttact ttgtatgaga aatcagacag attgggcggc
    44761 aggatatggg attatgaagg gattgccctc aaaaaggaag taatagagga agaaatagca
    44821 gccttagtga aacaggggat taaggcagag ttaaataccc ttattaataa aaaaaggctg
    44881 gaagagcttg ccggagaata tgatgcagta tacctgggaa caggtgtatg ggaggaggaa
    44941 cttaaaatcc atcctgagac ctttcaggta ggagatactt cggtgttcgc gggaggaaag
    45001 ctggcaaaca acaatgattc ggtaatattt tcggcaagtt cgggcagaag ggctgccgca
    45061 tccgttgaca gatatgtcaa gaaaatttcc ttgagcgctt ccagagagag agaagggtct
    45121 tttgagacgc ctttgaaata tgaaacagag ggtatcgaac ccgtcgaggc tataggcaag
    45181 acatctgata tttacagtga agatgaggct ataggggagg caggaaggtg tttgaagtgc
    45241 agatgcagaa aatgcattga atcatgcagc catttaacac gctttaatat atcaccgaaa
    45301 agctacatga ggcaaattaa tcaaaatgag aggattatac tgggaaccca ttatgcaaat
    45361 aaaatgatta attcctgtac aatgtgcggg ctgtgcaggg aagaatgctt caaaggcatt
    45421 agtatgaagg ctgtcataaa ggaaacaagg gagagcatgg taagaagggg caaaatgcct
    45481 gtttctgacc atgattttgc gctgaaggat atgaaattca gcaacagcaa gtacttttca
    45541 atggtgagaa atcagggcga aaaagctgag tacctgttct atccgggatg ccaactgcct
    45601 gcttcatatc cggagtacat agggaaaata tataaatatc tggtttccaa tatgtcgggc
    45661 ggtgccggaa tttacctggg atgctgcggg gctcctgccg attgggcagg gaggcaggat
//
LOCUS       DAOOAB010000019        42853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1138_length_42853_cov_14.651970, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000019 DAOOAB010000000
VERSION     DAOOAB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 42853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42853
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1138_length_42853_cov_14.651970"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..405)
                     /locus_tag="PKU88_05950"
     CDS             complement(67..405)
                     /locus_tag="PKU88_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPX68863.1"
                     /translation="MLYNETKKIIVVDEIKIAGSFKERLKGLMFKSRIGKNEGLLMLG
                     CNSIHTCFMRFAIDVVFMDLNNRVIFIKEKVKPWRSIGFIKKAYMTLELPEGTVKNKN
                     IAIGDILILS"
     gene            complement(419..1330)
                     /locus_tag="PKU88_05955"
     CDS             complement(419..1330)
                     /locus_tag="PKU88_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPX68864.1"
                     /translation="MLGAIMISTFSTVCFTMVMLLRIVFREKLHMQKRIKEYVFIDEK
                     SENILEGSFADRVIKPVMEKGNRFLGRIAPKHYYNYLERMINEAGNPKGITVGGVVLL
                     QIFSILVSAGAAVIMLLSTDIPTPKMLVLVCILLVLINIVPKFYLRHKISERQKEIER
                     GLPDVLDLLTVSIEAGLSFDGAVAKLVEKMSGVLAEEFSIVLKEMKVGVSKKDAFKSL
                     VERVPVPSLVTFVSSIIQADQLGVSIGNVLRIQSSQVRQKRMERTKENAMKAPVKMLL
                     PMIIFIMPTIFIVILGPVLIKIMEAFG"
     gene            complement(1333..2298)
                     /locus_tag="PKU88_05960"
     CDS             complement(1333..2298)
                     /locus_tag="PKU88_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPX68865.1"
                     /translation="MPEILLPMLFINMTLVIYLLYKYFSGKRSVAVRMKQYLNPEKNR
                     EKPEKERGLPWRESLFVFSKTLRKSKFGIKYREKIEARLVQAHLPIKPEEYMSINVIV
                     FLASALVFFVFTENLAGSAVFALLAARVPGIYVNSKRRSIIHKIDQQLPDTLALISNT
                     LKSGYSFLQAIDTASRELPPPISLEFQRILKEINLGTNTEKAMESLGKRVQSQDLELI
                     IMAVLIQRQIGGNLSEILDNISDTIRSRIKIKGEIKVLTAQGRISGIIISLMPVVLGV
                     ILYFINPSYISLLFKHPLGLAMLGTAVVMQGLGIYLIRKIVRIEV"
     gene            complement(2316..3635)
                     /locus_tag="PKU88_05965"
     CDS             complement(2316..3635)
                     /locus_tag="PKU88_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPX68866.1"
                     /translation="MGLLKRIEQEKTEILSEGRQSIKETKKEDPLSDVKGIIHKKIIE
                     RIDTSMLGQEGEEQRFNAEIADIVEETLKEKELHLSRTERQTMIRYIIDESIGFGPIS
                     GHLLDEEVSEIMVNGPDQVYVEKKGRLELADTIFEDNQHVMRIIEKIVSPLGRRIDES
                     SPMVDARLPDGSRVNAIIPPLALNGPTITIRKFAKNPLNVGNLISYGTMTPEMALFIE
                     ACIKSRLNIIVSGGTGSGKTTTLNVLSSFIPHDERIVTIEDAAELQLEQEHVVRLESR
                     PANIEGKGAISIRDLVKNSLRMRPDRIVIGEVRGGEALDMLQAMNTGHDGSLTTGHSN
                     SPRDMLSRLETMVLMAGMDLPVRAIREQIASAIDIVIHQARLKDGSRKITHITEVQGM
                     EGDTIILQDIFVLNEKGTFTATGIRPKFMEKLANTRVSLPNNLFSQV"
     gene            complement(3744..4937)
                     /locus_tag="PKU88_05970"
     CDS             complement(3744..4937)
                     /locus_tag="PKU88_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX68867.1"
                     /translation="MANRIKVVIVDDSEQTRDNIASIISFEKDIEIVGEAENGEEAIA
                     VVRSKKPNIVLMDINMPLLDGIKATQILNEEGADASIIIMSIQGEGEYLRRAMQAGAR
                     DYVVKPFGVNDLVKTIRHVYDLDMKRRQKSNVASVIKVDSRILSVFSTKGGVGKTTVA
                     TNLAVALSIVTGKKIALLDFDLQFGDVAICLNLYVKNSMTDLVKDYSNIQQDPGLLEE
                     YLLTHYSGIKILAAPTRPENAEYVTPEHIRNTIEFLKGRYDYIIIDTSHGFSDNAITA
                     LDMSDTIIYLSALDLPSIKNTKNGLEVMKSLNYPKEKVKIVINKSNERFGINHNDFEE
                     ALGVEIWAMIPDDQASVTISVNKGHPLISHRRSSPVSKKIYKLAQSIASGEEKAKKKL
                     FAAAF"
     gene            complement(4937..5674)
                     /gene="cpaB"
                     /locus_tag="PKU88_05975"
     CDS             complement(4937..5674)
                     /gene="cpaB"
                     /locus_tag="PKU88_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HPX68868.1"
                     /translation="MEGLNKKILALAIIMAFVTCMLLYFYISGLSSDSEIEYTEAYAA
                     SVEIPARTVVEDKMIVKVQVPKGTRIASGISDKSKIVGRLTKERIIKDELILPDRLYS
                     DEKTNMAFIIPTGKRAVTIGVNEVTEVGDFIIPGDYVDVIATFEEANVEIGGSKIYYP
                     KYTKIILQNVQVLGVGQNMQVLKEKDKDLPASVTLAVTPGEAERLVLADESGVLRLAL
                     KPAADNDTIQTNGVIKDDMVLPKGRVN"
     gene            complement(5699..6619)
                     /locus_tag="PKU88_05980"
     CDS             complement(5699..6619)
                     /locus_tag="PKU88_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HPX68869.1"
                     /translation="MNLRYYFNKRGNSSIILLFMVIVVIGLMTFSVDAGLLYFEKGRL
                     QSVVDSVALAAVSVYEEGEEKMLEEAHKYSNLNGVPPEELNIDISEDRKRVTVTTDKT
                     VALYFARIFDRDSADVGVSAAAVAGSIVSAKGIRPFAVEQQEFEYGETYTLKEGAGGG
                     HSGNYGALALGGNGASRYRNNLKNGYNASPPLRIGDEIETEPGNMAGPTQDGIQEILD
                     SDPNEHSEDLSQLEANCPRLIVIPVVDSLDVHGRSTATIVGFAAFFLDDVVRNGGHTE
                     ITGRFIKKIGEGEIDESSEGYGLFGIKLVE"
     gene            complement(6879..7262)
                     /locus_tag="PKU88_05985"
     CDS             complement(6879..7262)
                     /locus_tag="PKU88_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HPX68870.1"
                     /translation="MKLDKKGQSMVETALILPIIILLFLGMFEFSRIFGSYLLITHAS
                     REGARLAAIGRTDAEIQENIEGKVSILDESELQVILDPEEDDRITGEDVRVRVRYRLQ
                     IYAPVISSIVSNPFEMEAVTYMRVE"
     gene            complement(7371..7556)
                     /locus_tag="PKU88_05990"
     CDS             complement(7371..7556)
                     /locus_tag="PKU88_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HPX68871.1"
                     /translation="MMRLYNFWYDIKETLKDKKGQGMVEYALIIGLIAVVLITVLTNM
                     GTGIEDKFEEITNALTP"
     gene            complement(7839..8783)
                     /locus_tag="PKU88_05995"
     CDS             complement(7839..8783)
                     /locus_tag="PKU88_05995"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HPX68872.1"
                     /translation="MKRGDILLSDKEYIRHSLETNLFFLRIVKEHEIFAAASLPPRDR
                     GVMNQLVFMKNSTEQLLSEAIDLADRAVSHEVLASDELVTDYTLAAERATQFLTGISI
                     NTDLTRRELELRGSSKGRSAEGLQREISVLNRKAMAMTKAAVAFKTKLLKNVSECRAF
                     SYTYPTMLEHVIEEAEYYIMLLDKLEKRDGIDSIKEIVQEEINWNHIMEEHSKFIRGY
                     LDPEEEKLFDAANTFAKEFDRLLGKAEKAADNPELLPEVTEESIKNVIGLRNFKEKGT
                     EGILECRIKSVIPPLLADHVLREANHYLRLLKAFKEKL"
     gene            complement(8845..9342)
                     /gene="nrdG"
                     /locus_tag="PKU88_06000"
     CDS             complement(8845..9342)
                     /gene="nrdG"
                     /locus_tag="PKU88_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPX68873.1"
                     /translation="MELRVAGIVKESVVDGPGFRYVIFSQGCERRCKGCHNPDTHAMD
                     GGYVVETESIIEDIRQSRYIDGVTFSGGEPFLQTEAFVDIAEKLGEDNINIICYTGFT
                     YEELLGSKDESKIRLLGLIDTLIDGPYVEELKDLGLPYRGSKNQRMINVKSSLKQQEI
                     MLVNI"
     gene            complement(9354..11375)
                     /gene="nrdD"
                     /locus_tag="PKU88_06005"
     CDS             complement(9354..11375)
                     /gene="nrdD"
                     /locus_tag="PKU88_06005"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPX68874.1"
                     /translation="MFTKIRKRDGRVVDFTREKITGAIFKAARQVGGEDYETAENLTE
                     RVISYLYKHIKDEIPDVETVQDAVEKVLIETGHAKTAKAYILYRSKRTRIRETKSELM
                     DVVKDILIETSRDNANVGNSPSAKMLQIASAASKHYYLNNVIPEEMSKAHISGDIHIH
                     DLDYYGKTLNCLQIDLTKLLTDGFNTGYGFIRPPKRIGSAAAQAAIILQSNQNDMFGG
                     QSFPHFDKSMGEVIRNQCPNTDDDEIFQAMEGLIYNLNTMHSRAGAQVPFSSVNLGTD
                     TTPEGRKVTSAILTAFEKGLGKGESPIFPNLVFRLKEGVNMNAGDPNYDLFQFAMKVA
                     SRRMNPTFSFMDASFNSRFGDEVSYMGCRSRVIANRHGAEKSARRGNITPVTINLPKL
                     ALRTRDVDTFFVELNNILDLCSRQLIHRFNVISSLKVKDLPFLMGQKLYMGSENLKEQ
                     DEIREAIKNGTLAIGFIGLAEALTVLTGYHHGEDEESWKLGYQIVEGIKKAADNYSNE
                     YNLNFVAYATPAEGTSGRFIEKDRKEFGVIKGVTDKAYYTNSFHIPVNYQISAFEKIE
                     KEAPFHKLCAAGHISYVEMPSSPKDNLQAIEKIVKHMAECDIGYAGINFPIDECRSCG
                     VSGIIDSNCMRCGSEDIRRIRRITGYLSTIDRFNDAKQAELADRVKHAY"
     gene            complement(11387..11842)
                     /gene="nrdR"
                     /locus_tag="PKU88_06010"
     CDS             complement(11387..11842)
                     /gene="nrdR"
                     /locus_tag="PKU88_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPX68875.1"
                     /translation="MKCPYCGYSESKVVDSRPTVEEAAIRRRRECEKCSKRFTTYEKI
                     EEMPLIIVKKDGSREAYQRSKMLNGILRACEKRPISISTIEGMADEIEKELYNSMDKE
                     IESKKIGEMVMSKLKQIDDVAYVRFASVYRQFKDINIFLEELNKLIDER"
     gene            complement(12293..12523)
                     /locus_tag="PKU88_06015"
     CDS             complement(12293..12523)
                     /locus_tag="PKU88_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HPX68876.1"
                     /translation="MVKISELKQKDVININDGRRLGVVYDVEIDMEKGKIDALVVPGT
                     GKILGLFSKESDIVVNWGNIKKIGADVILIDG"
     gene            complement(12640..13410)
                     /gene="sigG"
                     /locus_tag="PKU88_06020"
     CDS             complement(12640..13410)
                     /gene="sigG"
                     /locus_tag="PKU88_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HPX68877.1"
                     /translation="MLNKVEICGVNTSKLPVLSNEKMVELLKKMQQGDQSSREEFIKG
                     NLRLVLSVIQRFNNRGEYVDDLFQVGCIGLIKAIDNFDLGQNVKFSTYAVPMIIGEIR
                     RYLRDNNSIRVSRSLRDIAYKALQVRDMLIGKNSKEPTVSEIANELKLPVEEVVFALD
                     AIQDPVSLFEPIYHDSGDAIYVMDQVSDDRNTDDNWLESIALKEAMKKLNDREKLILN
                     LRFFEGRTQMEVAEEIGISQAQVSRLEKTALGHMRKHI"
     gene            complement(13581..14276)
                     /gene="sigE"
                     /locus_tag="PKU88_06025"
     CDS             complement(13581..14276)
                     /gene="sigE"
                     /locus_tag="PKU88_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HPX68878.1"
                     /translation="MLIIRILQFFKVYKETWVHYIGGSEALPPPLENEEENFLLSRLK
                     GDEEVVKAVLIERNLRLVVYIARKFENTGVGVEDLVSIGTIGLIKAVNTFDPEKKIKL
                     ATYASRCIENEILMYLRRNNRTKTEISFDEPLNIDWDGNELLLSDILGTDNDIIYRYL
                     EDEIDKQLLEIALRKLSEREKRIMELRFGLNDGVEKTQKEVADMLGISQSYISRLEKR
                     IIKRLKKEISRMV"
     gene            complement(14364..15272)
                     /gene="spoIIGA"
                     /locus_tag="PKU88_06030"
     CDS             complement(14364..15272)
                     /gene="spoIIGA"
                     /locus_tag="PKU88_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HPX68879.1"
                     /translation="MKLETYVYADIILLENLIMNYLILWSTARLARYRHSKVKLLIAS
                     LLGAVYAVLSYFPGYGYLFTFYMKILFSLLIVIVAYTPAYFHLLLKLTGIFYIISFIF
                     GGAAFGLFYFINGLNLTSNGISFIRDFPVKILIAAVAAAYFIIRYSWDYIHHRIKREK
                     IILKVQMRFDGKQLCMDALVDTGNSLKDPLTNTPVMITEYDTIKELLPDDVQKIFEQE
                     GENDLNAIAEIMTISKWASRFRIIPFRSLGRENGMLVGFKPDEVIIFDKDRRIQSDII
                     IAIYRRNLSGDGEYGALIHPEMLKDR"
     gene            complement(15919..17004)
                     /gene="ftsZ"
                     /locus_tag="PKU88_06035"
     CDS             complement(15919..17004)
                     /gene="ftsZ"
                     /locus_tag="PKU88_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPX68880.1"
                     /translation="MLEFDVDINSIAQIKVVGVGGAGNNAINRMINAGLKGVEFYAIN
                     TDKQALHMSLAPNKIQIGEKLTKGLGAGANPDIGKRAAEESYDDIKKVLEGADMIFVT
                     AGMGGGTGTGAAPLVAQIARELGILTVGVVTRPFGFEGKVRKMQAEKGLTELKEKVDT
                     LVTIPNDRLLQIVDKKASIMEAFRIADDVLRQGVQGISDLIAVPGLVNLDFADVKTIM
                     LDKGYAHMGIGKANGENRSSEAVKQAIHSPLLETCIEGAKSVLLNITGGADLGIFEVN
                     EAADLVFQSADPDANIIFGAVIDESMQDEIRITVIATGFEEGAQRFFERRIEVAGLPE
                     EKGDGQKISNYGNEDIDIPTFLRNRYK"
     gene            complement(17122..17463)
                     /locus_tag="PKU88_06040"
     CDS             complement(17122..17463)
                     /locus_tag="PKU88_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HPX68881.1"
                     /translation="MIIPIIGLLLGIVIGILSPITIPIQYSSYMSVAILAALDSVFGG
                     IRSSIEKTFNIEIFMSGFFGNAVLAAILTYIGDQLGVPIYYAAIFAFGVRLFQNFAII
                     RRYVLMEHKKK"
     gene            complement(17463..18191)
                     /locus_tag="PKU88_06045"
     CDS             complement(17463..18191)
                     /locus_tag="PKU88_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPX68882.1"
                     /translation="MNRKAYIYYLFILAVIIGFIVTVQLKSNITYQGIITIPKLLDLQ
                     NEISNVEMENKLLKESISEHAMRLVEYKTSVEETGSAYGTMQNELEKARNYAGFYAVQ
                     GPGIIMTLNDSQQELAEGEDIAWYLVHDIDILEIVNELRVAGAEAISINGERVTATTN
                     IRCGGPTINIDGKRHAAPFIIRAIGDPKALEASALAPDSYIELYMEYTGIQVEIQKVE
                     KLVINGQNNLGKMKYQRKAEGGEA"
     gene            complement(18193..18912)
                     /locus_tag="PKU88_06050"
     CDS             complement(18193..18912)
                     /locus_tag="PKU88_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HPX68883.1"
                     /translation="MKNIKYQIAVAVVCMVLGIMVSIQFRTVKQGVGPVSEYRARELA
                     AQLKKVREENVKLQNVKNDYESKIKEFEDTASQGSLYAKILKEELDQARILAGIEDVE
                     GPGITVVVDDLKFSEKVNYPLISYSMLLELLNELNAAGAEAVSINEQRIISTSEIRQI
                     GGIHININTVSFAPPFVFKAIGDPKTLEAALRLREGIAEKLENSGVAVTITQEQLIKI
                     QKYNGVIERKYAKVVKEGESQ"
     gene            complement(18941..19657)
                     /locus_tag="PKU88_06055"
     CDS             complement(18941..19657)
                     /locus_tag="PKU88_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPX68884.1"
                     /translation="MRGRFKYVVLLFIICIFTAFFLSSSFFQIKYLVVNGNNNVTREE
                     IIKLSSIYYGENIFRINKRNSMKSIFQNPYVKMIKINRVLPDKVVIDIIERGIMAYVP
                     YVGSYLNIDEEGMILEINPAIKRTDLPLVKGLQFETFKVGEFLNVKNEEQFLTTTQLI
                     KVIKNAGMLNLVSEIDISDLSNIRLIIKEGIKANLGSADNMDYKINFARSVIEDVMKQ
                     NLKGTIEMSHNGNPVFKQEQ"
     gene            complement(19756..21009)
                     /gene="murA"
                     /locus_tag="PKU88_06060"
     CDS             complement(19756..21009)
                     /gene="murA"
                     /locus_tag="PKU88_06060"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPX68885.1"
                     /translation="MDKYVIIGGRRLEGDVRVDGSKNSILPVLAATIISGKESVIHNV
                     PELKDVDLLIGLLRTIGCRCFFENNTLVVKSNSQLNTIIPEKPVREMRSSIILMGAML
                     ARHGHVKISYPGGCEIGPRPIDIHLSGLRRMGTKITEAHGYINCECDRLKGVEINLDY
                     PSVGATENIMLAATTAEGVTIIQNAAKEPEIVDLQNFLNGMGARISGAGTSTVKIEGV
                     KEFHDVEHTVIPDRIVAGTLMAAAAITGGNIVLNDVIIDHLKLVSSKLSESGCSITEY
                     QNSIQITCSKKPKAVEVIKTLPYPGFATDMQAQMMAVMTIAKGTSIFIETVFESRYKH
                     VEELMKMGANIKVDGRTAVVRGVKKLTGTEVKAGDLRGGAALVLAGLAAEGTTVVDNV
                     KLHIDRGYDKLENKLAKLGADIHRV"
     gene            complement(21127..22245)
                     /gene="murG"
                     /locus_tag="PKU88_06065"
     CDS             complement(21127..22245)
                     /gene="murG"
                     /locus_tag="PKU88_06065"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPX68886.1"
                     /translation="MRAILSGGGTGGHIYPAVSIAKEIKNLYTDSEILFIGTDRGLES
                     SIVPKEGFKLVKIKVRGFLRKFSFGNVTAVAESFTGLFKVSKIIKDFRPDIVIGTGGY
                     VSGSVLLAAALMGIPTLIHEQNAFPGITNKILARLVDIIAVNFPEAKEYFPRKDKVIV
                     TGNPIRSTILSVSKEEGLKEFGFSRELPVVLVVGGSRGARKLNESVMLLAKKCVESKS
                     FQMLHMTGETQYDDILKKYDNENIPLDIGYLRVMPYVYNMPYALTASDIIISRCGAST
                     LSEITALGKPSILIPYPYAAGNHQEYNARALEKNGAAIVIQDRDLDADILYNEIAGML
                     NKPEKVSQMAAKSKKLGRTDAAYSIAKAAERLVQNKKA"
     gene            complement(22251..23363)
                     /gene="spoVE"
                     /locus_tag="PKU88_06070"
     CDS             complement(22251..23363)
                     /gene="spoVE"
                     /locus_tag="PKU88_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HPX68887.1"
                     /translation="MPARSKNNIDFTLLITVLILLAIGVAMVFSASSISSFMKYNDRY
                     HYLKSQGFFAILGILAMMFMSRFDYRILGRFAGPLVLLSIALLIAVFIPGIGYTANEA
                     TRWIKIGSQTMQPSEFAKFALIIFMAKSISNKKEKIRSFKHGVLPYVILAAIYFVLII
                     LEPNLSMAGSILMITFAMLFAAGTKIIYLIGWLIPVLPTVGYIIMKKPYMLARVTSYM
                     NPWADPLNKGYQAIQSLYALGSGGIFGLGLGNSRQKFFYIPEPQNDFIFAIIGEELGF
                     IGTATIVILFLMLIWRGLRIALACPDTFGCLLATGITCMIAIQATLNIAVATVSIPTT
                     GISLPFISSGGSSLLFVMVNMGILLNISKTVKTGRS"
     gene            complement(23427..24809)
                     /gene="murD"
                     /locus_tag="PKU88_06075"
     CDS             complement(23427..24809)
                     /gene="murD"
                     /locus_tag="PKU88_06075"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPX68888.1"
                     /translation="MQIKGKKVLVIGAAVTGVPVVKQLSRLGANIVLNDFKKVEELKE
                     VISEIQELPIKLVAGGHPARLAEDCDFVVISPGVPLDIPPVQRAKAMGKEVISEIELS
                     FRLTNTPIAAITGTNGKTTTTSLLGEIMRASGRKTYVAGNIGSAMINEVDKAKRPDIF
                     VLEVSSFQLESTIAFKPNISAILNITPDHLDRHKNMENYIDAKCRVFANQDEKDYTIL
                     NLDDPETSKLADRPKSKVLFFSRKKEVPQGVYMEKDYIVIKADNKETIIHKDKIYIPG
                     NHNLENALAAALMAYCAGVAPSVIADTLESFRGVEHRIEYVEELDGVIYYNDSKGTNT
                     DASIKAVEAMKRPTIIIAGGYDKGSEYDDFIEAFGDVVRHMVLIGKTADKLEETAHRH
                     GFMNTHKARDLREAVMKCHSLAEPGDCVLLSPACASWDMFSNYEERGRLFKEYVRELR
                     IRDQRYQAQN"
     gene            complement(24905..25882)
                     /gene="mraY"
                     /locus_tag="PKU88_06080"
     CDS             complement(24905..25882)
                     /gene="mraY"
                     /locus_tag="PKU88_06080"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPX68889.1"
                     /translation="MYNLVSGEYDIYIISIISAFVLALVAGPVFIPVLTRMKFGQIVR
                     DDGPQSHLKKTGTPAMGGIIIAVPAVIVSLAFSKDKDMLLVLITTMLFGIIGFIDDFL
                     KIKNRRSLGLKAYQKIILQLLVSVFLAVVASGISQVGTEVLVPFTGKFIDMGILYIPF
                     TVFVLISTVNCVNLTDGLDGLAGGTTVVVLGFFSVIALTSKNTGLLVFSGAMIGALLG
                     FLRFNSHPAQVFMGDTGSLALGGAIASLAVITRLPLFILIIGAIYVLEGLSVIIQVAY
                     FKMTGGKRFFKMAPLHHHFELSGWAESKIVAVFIISSIILSLIGILGLS"
     gene            complement(25889..27262)
                     /gene="murF"
                     /locus_tag="PKU88_06085"
     CDS             complement(25889..27262)
                     /gene="murF"
                     /locus_tag="PKU88_06085"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPX68890.1"
                     /translation="MMEALSMGEIARAAHGVLMNFEEESEISGVSIDSRKINPGDMFI
                     ALKGENFDGHDFIDMAIEKGAVLAVTQRLPERCKIPYILVEDTLKALQDIAAYYRSKF
                     NIPIVAITGSSGKTTTKDMIASVLTQRFNVLKTEGNLNNAIGLPLTLLKLQYSHQIAV
                     LEMGMSSLGEISLLSDIARPGIGVISNVGLTHIEKLGSRENILRAKLEIFSYFNSDST
                     AVINGDNDMLRDFCSDKFRVIKYGLKKGNDLFAYGIEEKGEAGIEFSVDLDGQSNFRV
                     LLPGIHNVYNALSAIAVARLFGMKAEEIQKGLDSFKPSEMRMEIIVLGSGVKLINDVY
                     NANPESMKVAIDVLRTMKSKGRGICILGDMLELGNLSSEEHCKTGAYAASSGADAIIA
                     VGEFSEDIKRGAAASGMNCNVYAFSDIKAAAASLDYIIRPGDTVLVKGSRGMKMEYIV
                     DYLRERG"
     gene            complement(27259..28716)
                     /locus_tag="PKU88_06090"
     CDS             complement(27259..28716)
                     /locus_tag="PKU88_06090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPX68891.1"
                     /translation="MKLEELLTGVDTVSFKGDLNIEINGIIYDSRKAKKGDLFICVSG
                     YKYDGHSYINDALQKGAAAFIVEKDVEVPGAAVVKVENSRAVMPMLASNFYGNPSQKL
                     KLIGITGTNGKTTTTYLIKSIMEANKQKIGLLGTISTQIGDKIYISSRTTPEALDLQC
                     LFREMADSDVDYAVMEVSSHSLELGRVEGSNFRIGIFTNLTQDHLDFHKTIENYGNAK
                     KKLFYMVSGTSIINIDDEHGKIIAAELKNNGKKLITYGIDNAADIMAKNIDITSKGVG
                     FTVITPEYEAELNISIPGKFSVYNALAAASAAYAEGLDMEVIREGLSRVENVPGRSEI
                     IKTNTPYTVIIDYAHTPDGLQNILASVKEYARGRIITLFGCGGDRDKEKRPIMGNIAG
                     RMSDYCIITSDNPRTEEPMDIIMQIEAGIKSTGCDYICIENRRDAIKFALATAKPDDI
                     VLLAGKGHETYQILRDRTIPFDERDIVEELLREEV"
     gene            complement(28913..30886)
                     /locus_tag="PKU88_06095"
     CDS             complement(28913..30886)
                     /locus_tag="PKU88_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HPX68892.1"
                     /translation="MSSIKVTIKRRIVIIFFAFFVIYAALLGRLAFIQIVNSAEYQQK
                     AVEQWTRDIPIRSKRGIIYDRNFKKLAVSATAYEIYVRPAMVKDKDAVTNALSEILGM
                     DKASLAQKVTANKDTVLIKKKVDAEKVKLLREKELQGIVFNDDSKRFYPQSNFASYVL
                     GFTNPDNQGQDGVELTYEKYLNGFPGRNIVMTDAHGEMLPGSDTKYYEPQDGLNLVLT
                     IDEVIQHFAEKVVENALIENKAKRVTAIVMDPKTGDILAMTSKPDFDNNDPRKVPEDL
                     ADKWSTMSEKEQLDVLYRIWRNPAISDSYEPGSTFKVITSSAGLEENVVKPEDHFNCI
                     GHITVAGKKMKCWRSYKPHGNQSFAEAVKNSCNPVFVEVGQRMGKEKLYKYIKAFGFG
                     SVTNIKLPGEARGIVRELGGVGPVELANNSFGQGISVTPIQLITAFSAVANDGMLMEP
                     RIAKKVIDGEETTVHEFQPRAVRQVISRETSATMRQILEFAIPPTASSYIPGYKVAGK
                     TGTAQKAEGGIYVSGKFVSSFCGFAPANDPKVSVLVVIDEPGAGQYYGGVIAAPVARS
                     IIYDTLRYMDVKPQYTAEEKELMQRPLVKVPEVRNMALKNAIKELEKSKLKYSVEAEF
                     EHNMESTIVDQTPKPGADINEGSIVILNAKPVQ"
     gene            complement(30919..31416)
                     /locus_tag="PKU88_06100"
     CDS             complement(30919..31416)
                     /locus_tag="PKU88_06100"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPX68893.1"
                     /translation="MLRADNTAYDYSRYEQLEMEQVREIAASPRKKVHKKEKHKTHLM
                     SILFVFGLSIFIISRYAYMAEINYNIKNLEKEYSALVKENTDLNVQLMKSISLETLEK
                     KAIEELGMQYPDIQNQIAYVNVEIPAQPAGNEARDFYNIDDLQENKYIAYTKAVINNI
                     LKVLD"
     gene            complement(31429..32379)
                     /gene="rsmH"
                     /locus_tag="PKU88_06105"
     CDS             complement(31429..32379)
                     /gene="rsmH"
                     /locus_tag="PKU88_06105"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPX68894.1"
                     /translation="MDFEHIPVLLNETLEYLDPKPGGIYIDGTLGGAGHSSEIAKRIV
                     PGGVLIGIDQDSNAIDAATRRLEAYKDNVIIVRDNFRNIKTIALQKGFKEVDGILLDI
                     GVSSHQLDEKERGFSYMQDGPLDMRMDTENGLNASDIVNNSSEQELIRILRDYGEEKW
                     AVRIAKFITEERKNSRIDTTFKLADIIQRAIPAAARRDGGHPAKRTFQALRIAVNDEL
                     QVLEEAVTNAARLLKPGGRLVVITFHSLEDRIVKKIFNNMEKPCTCPPQLPVCACGKE
                     PLLRVITKKPVTAGVEELKTNTRSKSAKLRAAERVSSKRG"
     gene            complement(32409..32840)
                     /gene="mraZ"
                     /locus_tag="PKU88_06110"
     CDS             complement(32409..32840)
                     /gene="mraZ"
                     /locus_tag="PKU88_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPX68895.1"
                     /translation="MFIGEYNHTLDPKNRVIMPSKFREKLGDSFVMTKGLDNCLFIYS
                     SRDWSIVEDKLKSLPMTNKDARAFVRFFFAGACECDLDKQGRMVIPNYLKEHANIDKE
                     LVIIGVSTRIEIWSKEEWNKFNNDANISYEDVAEKMSQLGI"
     gene            complement(33141..33908)
                     /gene="lgt"
                     /locus_tag="PKU88_06115"
     CDS             complement(33141..33908)
                     /gene="lgt"
                     /locus_tag="PKU88_06115"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPX68896.1"
                     /translation="MDPIAFEIYGIAIRWYGILLSAGIIAGILLAYYEAKRLGYDPEY
                     IIDLALWCVPAAVIGARLYYVIFEWSYYKGDIMKIINIRGGGLAIHGALIAAVITGYV
                     FTRVKKISFWNTADITAPSIILGQAIGRWGNFVNQEAHGEPTDLPWGIMVDGVKVHPT
                     FLYESLWNICVFIFLLIYRKKKKAEGEVFLLYGILYSVGRFWIEGLRTDSLMFMGMRQ
                     AQIISVVIIAAFTALIVYRRKKNKDKAGEDTEDIAEE"
     gene            complement(34049..34867)
                     /locus_tag="PKU88_06120"
     CDS             complement(34049..34867)
                     /locus_tag="PKU88_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HPX68897.1"
                     /translation="MTNKKRLNLLKGIEKMRNSRLLSYISFSPMDDYVLVPLYKQLCS
                     IGKTERIDLFLNSYGGAVDTPYKIVNLIREFCDNFSVIIPFVAKSAATMVAIGADEIV
                     MGPISELGPIDPLVRHPLFTDLWVPVQSIRLCIDFMEEKLAQSKDTKITASVLYPISN
                     KLDPWILGDYEKSIKASYQYAEALLENNMFKDNKDRAKTVTKTMTEKYFSHGYCINRK
                     EALNDLGLKIVYADGDMWHLIWALYLAYDDYMQGKNFMSVIETVNGVLDLPNSD"
     gene            35056..35676
                     /locus_tag="PKU88_06125"
     CDS             35056..35676
                     /locus_tag="PKU88_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68898.1"
                     /translation="MPPLNDGELTSMKLSIINNLEKTLKIVYEHQYRNILSKIVSNKK
                     RFIRKRLPLDASEDMCRYNSLAAGLTNYLINLFDVFGESAVLNAKKTFEENGSKWGKK
                     LRKKLLMQSDTNDINHIVKYLYIDVPEIDYMEMSDNGLVWHFNKLGHSSINEGFAKFH
                     PAFYNIKSAWLHSFIKSFTAQYTGIFEKKSEDDNEIVTRIEFKEDQ"
     gene            complement(35942..36685)
                     /locus_tag="PKU88_06130"
     CDS             complement(35942..36685)
                     /locus_tag="PKU88_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008701579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPX68899.1"
                     /translation="MENKINKWEPENFELEMNTVWSFPIRGDWATHDAKYRGNWSPYI
                     PRNIILRYSQEGDLILDQFAGGGTTLVEAKLLNRNIIGMDINDVAIERCHEKCNFERE
                     NSGQVTIAKGDARKLDSIINESIDLICTHPPYANIIQYSDDISEDLSQCKVPEFLVEM
                     KQVAVESYRVLKKNRFCAILMGDMRQKGHVIPLGFKVMNLFEEAGFKTKEIIIKEQHN
                     CKATGYWKTNSVKYNFLLLAHEYLFVFRK"
     gene            complement(36736..37596)
                     /locus_tag="PKU88_06135"
     CDS             complement(36736..37596)
                     /locus_tag="PKU88_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPX68900.1"
                     /translation="MARDFNLWFSKFRDSISDYGYYVDFGKVHRNVNGIKVELNILNS
                     LIGSDNIEDDFEALVYKYPETLKCIPILLAVRANEIRAMDTEGSYNYSFLRMNYSIEQ
                     YKVFMHKTGLFDLIKNHIVSNLLDYVTGIETGLDSNGRKNRGGHLMEELVESHIVKSG
                     FIDGIDYFKEMNLSDIERKWSINLSAISNQGKAEKRFDFVVKTGNMIYGIETNFYSSS
                     GSKLNETARSYKTLALEADTIDGFTFVWFTDGKGWNSARRNLEETFDVMKHLYSIDDM
                     ENGVMDDLFE"
     gene            complement(37615..38955)
                     /locus_tag="PKU88_06140"
     CDS             complement(37615..38955)
                     /locus_tag="PKU88_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68901.1"
                     /translation="MSELDVFARGIAEICPDNPESLTIEQARNVVKQINDFLYANYEG
                     IGSVEALGEEFEFISDFHKYWYEHHREILNIEIDENVCHDVASVLHEVYIRTGGRVFF
                     EIYDTNGLSNEDTCRIRFLTANQDFRGSRSFRELASVFLSDNSIFDEQAIFNDPADFV
                     RSIGITGLSQSDKRVQFAKNISKFLLDRSVQPYNVIDYYDRDVFELRNAIICEPNAGY
                     GNKKADMLIRDMVVLGVWDNVTGFEKIDVASDINTIKVALRTGIIKAAIPLVSSFIDI
                     FCYQYGYVDELNALAWRKVWEIWCNEYPNEVIGSPCLLDYFVYNVIGKQFCKEILCEF
                     QCDVENHTFKWHSSRNRTCQICYTNGIRNKKATLIKKILPCTDNDGSIAIRETDFVRS
                     NIADPNYDTCPFREICISADNMGLEPPKSISIKGQTGWITAYARKNQGGGGLMS"
     gene            complement(38965..39927)
                     /locus_tag="PKU88_06145"
     CDS             complement(38965..39927)
                     /locus_tag="PKU88_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPX68902.1"
                     /translation="MCIIPTDAKKPIDWRSLKHKKYQTISLKPFVKWAGGKGQLLTDI
                     RKTYPVGLGVDIKKYAEPFVGGGAVLFDILSSYNLDAMYISDINAELINAYRMIRDEG
                     DELIGLLSEYQNEFIPLEDAQRKEYYYAKRKRFNELKINGVSSINLESAAVFIFLNRT
                     CFNGLFRVNRKGLFNVPMGAYKKPTICDAENLLNISYSLQNVEIICGDYRKSSGFIDK
                     STFVYFDPPYRPLNTTSSFTSYTEDGFDDKAQRELAEYVNLLTKRGAKVIVSNSDPKN
                     ENSEDEFFDELYSSHRIKRVEASRMINSNAESRGKISELLICNF"
     gene            complement(40212..40868)
                     /gene="deoC"
                     /locus_tag="PKU88_06150"
     CDS             complement(40212..40868)
                     /gene="deoC"
                     /locus_tag="PKU88_06150"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPX68903.1"
                     /translation="MNKFELARYIDHTLLKPDAKEGDIIKLCKEALEYKFASVCVNAS
                     YVKLAYSFLQGTEVRVCTVAGFPLGATTKEAKAFEASQAIENGAAEIDMVINIGALKS
                     GKLDAVKEDIREVAGVCKDKALLKVIIETCLLTDEEKVTACLLSMEAGADFVKTSTGF
                     SVGGATAEDIKLMRRTIGPDMGVKASGGIRNLESALKMIEAGASRIGASASVSIVNEL
                     "
     gene            complement(40895..41467)
                     /locus_tag="PKU88_06155"
     CDS             complement(40895..41467)
                     /locus_tag="PKU88_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPX68904.1"
                     /translation="MEKFIPVNMSNRHIHVTKEHLEILFGEGHELTNIKDLSQPGQYA
                     CDEKVDIVGPKSTLKGVRILGPVRPDTQVEISMFDARTMGVEGMVRPSGKIQGTPGCT
                     IVGPKGKVELKEGVIVAARHIHLHTSDSERFGLKDRDVVKVRIGDERALVLENVMVRV
                     HPTYAMDMHIDIEEANAAGIQNGALGEIIR"
     gene            complement(41489..42841)
                     /gene="scfB"
                     /locus_tag="PKU88_06160"
     CDS             complement(41489..42841)
                     /gene="scfB"
                     /locus_tag="PKU88_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HPX68905.1"
                     /translation="MLHRYEQKGLKIVVDVNSGAVHVVDQLTWDILDHYPDSVENIVG
                     VLKGKHEECNIREAWNEVRELESQGLLNSADVYMDKVLEDKKEMYTKALCLNVAHDCN
                     MRCGYCFASTGDYHEGRRLMPFSVASRAVEFLLESSGNRKRLEVDFFGGEPLMNFDVV
                     KETVLFAREREKEYGKKIGFTITTNGTLLDKETAEFINENMDNIVLSIDGRKQVNDRM
                     RKFTCGAGTYDDIMPKLKSFVASRGDKSYYIRGTFTANNLDFCSDVLHLADEGFKEIS
                     IEPVAAEKGKAYALKEEHLPRIYREYDRLAEKYIDYYEKGKGFRYYHFLMDLDGGPCV
                     YKRVSSCGSGVEYFAVTPDGELYPCHQFVGRKEYLMGDVWKGVSNKELQKEFSGNTVY
                     HKEKCRECWARFYCSGGCQANAAAFNNNLKEPYDIECKLQKKRIECAIMIKAYEKLKN
                     "
BASE COUNT        11119 a        10736 c         7414 g        13584 t
ORIGIN      
        1 tcccttgagg gacacgttaa acgtccccgt gtctcccccg tgtctccccc gtgtccccgt
       61 atcccatcag cttaggataa gaatatctcc tattgcgata tttttattct taaccgtacc
      121 ttccggcagc tccagagtca tatatgcttt ctttataaaa ccgatgctgc gccacggttt
      181 tactttttcc tttataaata ttaccctgtt gttcaggtcc atgaaaacaa catctatggc
      241 aaacctcata aagcatgtgt gtatggaatt gcaccccagc ataagaagcc cttcgttttt
      301 gcctatccgg cttttgaaca taagcccttt gaggcgctcc ttaaagctcc cggctatttt
      361 gatttcatca actacaatta ttttttttgt ttcattgtac agcataagtg cctcccttct
      421 atccgaaggc ttccatgatt ttaatgagaa caggccccag aatcactatg aatatagtag
      481 gcattatgaa tattatcatg ggaagcagca tttttacagg cgccttcata gcattttcct
      541 ttgttctctc cattcttttc tgccttacct gggaggattg tattctaaga acatttccta
      601 tgcttacgcc cagttgatca gcttgtatga ttgagcttac aaaagtaaca aggcttggca
      661 ccggcaccct ttcaacaaga gatttgaagg catccttttt tgataccccc actttcattt
      721 ccttcagcac tattgagaat tcttccgcaa gcactccgga cattttttca accagctttg
      781 caactgcccc gtcaaagcta agtcccgcct ctatggatac agtaaggagg tccagcacat
      841 ccggaaggcc cctttctatt tccttctggc gttcacttat tttgtgcctt agataaaatt
      901 taggaacaat atttatgagc acaagaagta tgcataccag gacaagcatc ttcggcgtgg
      961 gtatatccgt ggaaagcagc ataatcacag ctgcccctgc cgagacaagg attgagaata
     1021 tctgaagaag tacaactcct cctacggtta ttcccttcgg attccctgct tcattaatca
     1081 ttctctccaa ataattataa tagtgtttgg gtgcaatcct gcccaagaat ctgttgccct
     1141 tttccataac cggctttatt acccggtctg caaaggaacc ttcaagaata ttctctgatt
     1201 tttcatctat aaatacatac tccttaattc ttttctgcat atgaagcttt tctctgaaaa
     1261 caatccttaa aagcattacc attgtgaaac atacggttga aaacgtactt atcataatcg
     1321 ccccaagcat tgctacacct caattcttac aatttttctt ataaggtata tgcccaaacc
     1381 ctgcatcaca acggctgtac ccaacatggc gagacccagg gggtgcttga ataacagtga
     1441 aatataagat ggattaatga agtacaatat aactccaaga acgacaggca tcagtgatat
     1501 gataatgccg gagattctcc cctgggctgt cagcaccttg atttctccct ttatttttat
     1561 ccttgagcga attgtatccg aaatattatc caatatttcc gacaggttgc cccctatttg
     1621 cctctgtata agaaccgcca ttattattag ctccaggtcc tggctttgga ctcttttgcc
     1681 caggctttcc atagcttttt ctgtattggt tcccaagttg atttccttca atatcctctg
     1741 aaattcaagg gatattgggg gaggaagctc cctggaagcc gtatctattg cctgaagaaa
     1801 gctgtatcca gacttcaggg tatttgagat aagggctaat gtatccggca gctgctggtc
     1861 tatcttatgt ataatgcttc ttctttttga attcacataa attccgggca cgcgggctgc
     1921 cagcaaagca aaaacggccg aacctgcaag gttttccgta aatacaaaaa agacaagggc
     1981 agatgcaagg aatactataa catttattga catgtattcc tccggcttta tgggcagatg
     2041 cgcctgaaca agcctggctt caatcttctc cctgtacttg attccaaact tgctctttct
     2101 cagagtctta ctgaaaacaa acaagctttc ccgccagggc agccctcttt ctttttcagg
     2161 tttttccctg tttttctcgg ggttaaggta ttgcttcatt cttacggcta cgcttctctt
     2221 tcccgagaaa tatttataca gcaagtatat gacaagggtc atatttataa agagcatagg
     2281 cagcaagatt tccggcataa tgaatccctc ttttctcata cctgactgaa caggttgttt
     2341 ggcaaggata cccttgtatt tgcaagcttc tccatgaatt tgggccttat tcctgtcgcg
     2401 gtaaaggtac ccttttcatt cagcacaaat atgtcctgaa gtattatggt atccccctcc
     2461 attccttgaa cttccgtgat gtgggttatc ttcctgcttc catccttaag ccttgcctga
     2521 tgtattacga tgtcaattgc tgaagctatc tgttccctta tagctcttac aggcaggtcc
     2581 atgcctgcca tcagcaccat ggtctcaaga cgtgaaagca tatccctggg agaattggag
     2641 tggcctgtgg taagtgaacc gtcatggcct gtattcattg cctgcagcat atccaaggct
     2701 tctccccctc tgacttcgcc tattactatc ctgtcaggcc tcatcctcag tgaattcttt
     2761 acgagatccc tgatactgat ggctcctttt ccctcaatat ttgctggcct gctctcaagc
     2821 ctgaccacat gctcctgctc cagctgcagc tctgctgcat cctctatggt tactatcctc
     2881 tcatcatgtg gtatgaagga ggaaagtaca ttcagggttg tagtttttcc gcttccggta
     2941 ccccctgaaa ctatgatatt aagccttgac ttgatacaag cttcaataaa cagcgccatt
     3001 tccggggtca ttgtaccgta agaaatcaga ttgcccacat tcagaggatt cttcgcaaac
     3061 tttcttattg ttattgtcgg cccgttcaga gccaaaggcg ggattatggc atttaccctg
     3121 gagccgtcag gaaggcgcgc atccaccatg ggagagcttt catctatcct tcttcccaag
     3181 ggggacacta ttttttctat tatcctcatc acgtgctgat tatcttcaaa tattgtatcc
     3241 gccagttcaa ggcgcccttt tttctccaca tagacctggt caggcccgtt gaccattatt
     3301 tccgaaactt cctcatccag aagatgcccg ctgatggggc cgaagcctat ggactcgtca
     3361 ataatatacc ttatcattgt ctgccgttcg gtacgggaaa gatgcagttc tttttccttc
     3421 agagtttcct ccacaatatc ggctatttcc gcattgaacc tctgctcttc accctcctgc
     3481 cccagcatgc ttgtgtcaat tctttcgata atctttttat ggattatccc ttttacatca
     3541 cttaagggat cctctttttt tgtttccttg atcgattgtc ttccttccga caatatttcg
     3601 gttttttcct gctctattct tttaagcaaa cccatattta ttacctcctg ggcattccta
     3661 tatagtctgg tacgagacac gaggtgcgag gtttggtgtt ttcttcgaga acctgctcct
     3721 atcctcgtgc cccgtgccca attttaaaac gccgccgcga acagcttctt cttagccttt
     3781 tcctcaccgg atgctatact ttgagcaagc ttgtatatct tttttgacac cggtgacgac
     3841 cttcggtgag atataagggg atggcccttg ttcaccgata tcgtaacaga tgcctgatca
     3901 tccggtatca tcgcccatat ttccacccca agggcttctt caaaatcatt atggtttatg
     3961 ccgaagcgtt cattggattt gttaatgaca atcttcacct tctctttggg gtaattcagg
     4021 gatttcatta cctccaggcc atttttcgta ttttttattg acggaagatc cagggctgat
     4081 aaatatataa ttgtgtccga catatccaga gcagttatgg cattatcgct gaagccatgg
     4141 gaggtatcaa ttattatata atcatacctt cccttaagaa attcgatggt gttcctgata
     4201 tgttcaggag taacatattc tgcattttca gggcgtgtag gagcggccag aatctttata
     4261 cctgaataat gggtgagaag atattcctcc aataagccgg gatcctgctg tatatttgaa
     4321 taatccttta caaggtccgt catggagttc ttgacgtaaa gattcaggca tattgccaca
     4381 tctccaaact gcaggtcaaa atccagcagt gctatctttt ttcccgtaac aattgacagt
     4441 gctaccgcca gattggttgc tacggtagtc ttccctaccc cgccctttgt agaaaatacg
     4501 ctcagtatcc tggaatctac cttgattacc gaggcaacgt tacttttctg cctccttttc
     4561 atatccagat catatacatg ccttattgtt ttgaccagat cgttcactcc gaagggcttc
     4621 actacataat ccctggctcc tgcctgcatc gcccttctta aatattctcc ttcaccctga
     4681 attgacataa taattattga cgcatccgca ccctcttcat taagtatttg tgtggctttt
     4741 atcccatcca gcaaaggcat gttgatgtcc atcagcacaa tatttggctt cttgcttctt
     4801 acgaccgcta ttgcttcctc tccgttttca gcctcgccaa caatttcaat atccttttca
     4861 aagctgataa tagacgcaat attgtccctg gtctgctcgg aatcatcaac aataacaact
     4921 tttatcctgt ttgccattaa ttcaccctcc ctttgggcaa taccatatca tccttaataa
     4981 caccattggt ctgaatagta tcattatccg ctgccggctt caaagcaagc ctgagtacac
     5041 cgctttcatc agcaagaaca agcctctccg cctcacccgg agttacagcc aaagtcacgc
     5101 tggcaggaag atccttgtct ttttccttaa gaacctgcat attctggcct actcccagta
     5161 cttgcacatt ctgcagtatg atctttgtat atttgggata gtatatcttg cttccgccta
     5221 tttcaacatt tgcttcctca aaggttgcaa taacatccac ataatccccg gggattataa
     5281 aatcccctac ttcggtaacc tcattcactc ctattgttac ggcacgtttt ccggtaggta
     5341 ttataaaggc catattggtc ttttcatcac tataaagcct gtccggaagt atcagctcat
     5401 ctttaataat tctttccttc gtcagccttc ccactatctt gctcttgtct gatatacccg
     5461 atgcaattct tgtacccttc ggaacctgca ccttcacaat cattttatct tcgactaccg
     5521 tcctggcagg aatttcaacg cttgccgcat atgcttcagt atattcgatt tccgaatcgc
     5581 tgctcagccc tgaaatatag aaatacagca gcatgcacgt aacaaaggcc attataattg
     5641 ccagagcaag tattttttta ttcaaacctt ccattatata atccccctct gtcaaacact
     5701 actccaccag ctttatgccg aataatccgt atccttcact gctctcgtca atttctccct
     5761 ctccgatttt ctttatgaat cttcctgtta tctctgtatg cccgccattt ctaaccacat
     5821 catccaaaaa gaaggccgca aagcctacaa tagtagccgt acttcttcca tgtacatcca
     5881 gggaatcaac gacaggaatc actataagcc tgggacagtt tgcctcaagc tgtgaaaggt
     5941 cctcactatg ctcattgggg tcggaatcaa gtatttcctg tatgccatcc tgggtaggcc
     6001 ccgccatatt tcccggctca gtctcaatct catctcctat tcttagaggc ggactggcgt
     6061 tatatccatt tttcagattg ttcctatatc tacttgctcc gtttccccca agagccaaag
     6121 ctccatagtt accgctgtgc cctcctccgg caccttcctt cagggtgtaa gtctctccat
     6181 attcaaactc ctgctgttct actgcaaaag ggcggatacc tttagctgac actatagaac
     6241 ccgccactgc cgcggcacta accccaacat ccgcactgtc cctgtcaaaa atcctcgcga
     6301 aatacagagc aacagtctta tcggttgtca cagtcaccct ttttctgtct tcggatatat
     6361 ctatattcaa ttcctcagga ggcacaccgt tcagattgga gtacttgtgc gcctcctcca
     6421 gcattttttc ttctccctct tcatagaccg aaacagcggc taaagccaca gaatcaacta
     6481 cactctgcag cctgcccttt tcaaaataaa gcagcccggc atctacagaa aatgtcatca
     6541 gccctatgac aacaatgacc ataaacagaa ggattattga actgttccct cttttattga
     6601 aatagtatct taaattcata ttaccctcac ctcattcgct ctaccctgat agtatgagtc
     6661 accgaaaacg actataagca agcaagacaa ggcgtgcaag gcggaatgcc ctgggtcact
     6721 catagggggc attcccccct gcatgacgga tgcaagcatc ctgtcacccc cctttcggca
     6781 gtcaagctat cgcgggtctt ctctcaagac acactgtatg tcaggacttt ccgaccgcag
     6841 cacaacgcag tattgcgccg cttatcgtcg ttttcgcttt actctaccct catgtaagta
     6901 acagcttcca tctcaaaagg gtttgagact atactgctga taacgggagc ataaatttgc
     6961 agcctgtatc ttacgcgcac ccttacatcc tctcccgtta tacggtcatc ctcctccggg
     7021 tccaatatta cctgcagctc gctttcatcc aatatactga ccttgccttc aatgttttcc
     7081 tgtatctcag catctgtcct tcctatggcc gccagccttg ctccctccct tgacgcatga
     7141 gttataagaa ggtaggagcc aaatatcctt gaaaactcaa acatacccag aaaaagcaaa
     7201 atgataatag gcaatatcag agccgtctcc accattgact ggcctttttt atcaagcttc
     7261 atggcttccc tcctcttgtg aatagtaaat tatagaaaga ctgaccccgc ctggtaaaag
     7321 gcagggtcta caatccatta ttatgtcaca aaaaacattc ttatatcggt ttatggagta
     7381 agtgcatttg tgatctcttc aaacttgtcc tctattccgg tacccatatt tgttagaact
     7441 gttataagca ctactgcgat caggccgata atgagtgcat actccaccat accctgtcct
     7501 tttttgtcct ttaaagtttc ttttatgtca taccagaaat tataaagcct catcatcatt
     7561 ggaactccct ccttcatata aatttttatg tgcttttatt ctactcataa ttataacctg
     7621 acaccatccg acaaattatt catttttcgt tgcggcacaa ttgcccacgt catgagtact
     7681 cttgcttatg ctgaatattt atacaagtcc tattaataaa taaaagtact atgaaatttc
     7741 atagtacttt ttgatgatat cattataggc aatatggtca ttgaacaggc tataagtctt
     7801 agatagtccc atttcgcaca aggcacgcaa aaatgattct aaagcttctc cttaaaggct
     7861 ttcagcagcc taaggtaatg atttgcttcc ctcagcacat gatctgccag cagcggaggt
     7921 atcaccgact ttatcctgca ctccaatatc ccctctgtgc ccttttcctt gaagttccta
     7981 agtcctataa cattcttaat gctctcctcc gtaacctccg gaagaagctc cggattatcg
     8041 gcggcttttt ctgcctttcc cagcagccgg tcaaattcct tggcaaaagt attggcggca
     8101 tcgaacagct tctcctcttc aggatccagg tatcctctga tgaacttgct gtgctcttcc
     8161 atgatatgat tccagtttat ctcctcttga actatttcct ttatgctgtc tataccgtct
     8221 ctcttctcca gcttatcaag cagcatgata taatactccg cctcttctat tacatgctcc
     8281 agcattgtag gataggtata actgaatgcc ctgcactccg atacattttt caggagcttt
     8341 gtcttaaagg cgacggcagc tttggtcatt gccattgctt ttctgttaag caccgagatc
     8401 tccctttgaa gcccttcagc gcttctgccc ttacttgaac ctctcagctc aagctctctt
     8461 cttgtaagat cagtatttat cgaaatgcct gtcaagaact gagttgccct ttccgcagcc
     8521 agagtataat cagtaaccag ctcatcggat gccagcactt catggcttac agccctgtcc
     8581 gccaggtcaa tggcctcgga cagcagctgc tcggtactgt tcttcataaa caccaattgg
     8641 ttcatgaccc cccggtccct tggaggcagt gaagccgctg caaaaatctc atgctcctta
     8701 actattctta aaaaaaacag attcgtttcc agtgaatgtc tgatatactc cttgtctgat
     8761 aacaaaatat cccctctttt cattgctatg gccctgcaat gcagtatcat taccatagta
     8821 ttccggagag gatatatgtg ttacttatat attaacaagc ataatctcct gctgcttcaa
     8881 actgcttttt acattaatca ttctctgatt ttttgagcct ctgtaaggaa gccccaaatc
     8941 tttcagctct tccacataag gcccgtcaat aagggtgtca atgagcccaa gaagcctgat
     9001 cttgctctca tccttgcttc ccaaaagctc ttcatatgta aagcccgtat agcatataat
     9061 atttatattg tcttccccaa gcttttctgc aatatcaacg aaggcctccg tctgaaggaa
     9121 gggttctcct cctgagaagg taactccgtc tatgtatctg ctttgcctga tatcttcaat
     9181 aatgctttct gtttctacca catagcctcc gtccatcgcg tgagtgtcag ggttgtgaca
     9241 gcctttgcat ctacgctcac agccttggga aaatatcacg tatctgaatc cggggccgtc
     9301 cactacgctt tccttgacaa taccggccac tctcaattcc ataatgacgc ctcctaatat
     9361 gcatgtttaa ccctatccgc caattctgcc tgtttagcat cgttgaacct gtcaatagtg
     9421 gacaagtagc ctgtaatcct cctgattctt ctgatatctt cacttccgca cctcatgcag
     9481 ttgctgtcta ttattcccga cacgccgcag cttctgcact catctattgg aaagttgatc
     9541 cctgcatagc caatatcaca ctctgccatg tgctttacaa tcttttcaat ggcctgaaga
     9601 ttgtccttcg gtgaagaagg catctctacg tatgaaatat gccctgccgc acacagctta
     9661 tggaaggggg cttctttttc aatcttttca aatgcgctta tctgataatt gaccgggata
     9721 tggaaggaat tagtataata tgccttgtcg gtaactccct ttatcactcc gaattctttc
     9781 ctgtccttct caataaacct tccgctggtt ccttccgccg gggtagcata agctacaaaa
     9841 ttcaggttat actcgttgga atagttgtct gcggctttct taataccttc cactatctga
     9901 tagcccagct tccatgattc ttcgtcttcc ccgtggtgat agcctgtaag cactgtaagc
     9961 gcttcggcaa gtcctataaa acctattgcc agagtaccat tctttattgc ttctctgatt
    10021 tcatcctgct ccttcaggtt ctcggagccc atataaagct tttgtcccat caggaatggc
    10081 aaatccttta ccttgagaga cgagatgaca ttgaatctgt gaataagctg cctgctgcac
    10141 aaatccagaa tgttattcaa ttcaacaaag aaagtatcaa catctcttgt tctcaaagcc
    10201 agcttgggca ggtttattgt aaccggtgtt atatttcctc ttcttgctga tttttcagct
    10261 ccgtgcctgt ttgcaataac tcttgaacga cagcccatgt aactgacctc atctccgaaa
    10321 cgcgagttaa agctggcatc cataaagctg aaggtagggt tcatccttct ggatgcaacc
    10381 ttcatggcaa actgaaacag gtcataattg ggatctccgg cattcatatt taccccttcc
    10441 ttcaatctga ataccagatt ggggaatatg gggctttcac cttttcccag gcctttttca
    10501 aaggcagtca agatcgcact ggttaccttc cttccttcgg gagtggtatc tgtgccgagg
    10561 tttacgctgc tgaagggcac ctgggctccc gccctgctgt gcatcgtatt caggttgtat
    10621 atcaagcctt ccatagcctg aaatatctca tcatcatccg tattggggca ctggttcctt
    10681 attacctctc ccatgctttt gtcaaaatgg gggaagctct gccctccgaa catgtcattc
    10741 tgattgcttt gaagtattat tgcagcctgc gcagctgcag agcctattct tttcggaggc
    10801 ctgataaatc cgtatccggt attgaaaccg tctgtcaaaa gctttgtcag atctatctga
    10861 aggcagttta gagtcttgcc atagtaatcc agatcatgta tgtgaatatc acctgaaata
    10921 tgcgccttgc tcatttcttc aggtatgacg ttgttaagat aataatgctt gctggctgcc
    10981 gaggcaatct gcagcatctt ggcggagggc gagtttccga cattggcatt gtccctgctg
    11041 gtttctatca gtatatcctt aactacgtcc atcaactcgc tttttgtctc tctgatcctg
    11101 gttctcttgg atctgtataa tatgtacgct ttggcagtct tggcatgccc tgtctcaata
    11161 agaacctttt ccacagcatc ctgtactgtt tccacatcag gaatctcatc cttaatgtgc
    11221 ttataaagat aggatattac tctttctgta agattctctg cggtctcgta gtcttctccc
    11281 cctacttgcc gcgctgcctt gaatattgca cctgtaatct tctccctggt aaagtctact
    11341 accctaccat ctcttttgcg tatcttcgtg aacattcatc taccccctat ctctcgtcta
    11401 ttaatttgtt cagttcctcc aaaaaaatat ttatatcctt gaattgtctg tataccgatg
    11461 caaaccgaac ataagcaaca tcatctatct gcttcagttt gctcataacc atttctccga
    11521 tttttttgct ttctatctct ttatccattg aattgtaaag ctctttttcg atttcatccg
    11581 ccatcccttc aatagtggaa attgaaatag gcctcttctc gcaggctctt aatattccat
    11641 tcagcatctt gcttctctgg taggcttctc tgcttccgtc tttctttaca ataatcaaag
    11701 gcatttcttc tattttttca taggttgtaa atctcttgga gcatttttcg cattcacgcc
    11761 ttcttcttat tgccgcttcc tcaacagtag gccttgaatc cactaccttg ctttcactgt
    11821 agccgcaata agggcacttc acactgcttc ctcccttcta gtgcctcgtg aggcactgaa
    11881 tctatgtgat gattattgta ttaataatca ctagtatatg gaaaattttg tcgcaataac
    11941 agagcttgca atactatttc catgagtctc agttcctatt tttgcaccaa tatattgtac
    12001 catatactat tatattatac atgatatagt aaaatcaccc ttcccatttt tccaaattta
    12061 taaagtaata tatgttatct ccgtaatcct ttttatatct cttcttgtca taaaaatgga
    12121 aaaaagcgac tcacgtcgct ttttttcgct agggaaccct atggttccct tcgacggatg
    12181 taagcatcct gaaactcctt tatcggcagg gggcttctcc cccttgcatc ccccatcgta
    12241 taggaaatat gctttcctat acgataaaaa caggagactt tcttttctcc cactaaccat
    12301 ctataagaat cacatcagca ccgatttttt taatattccc ccaattgact actatatcgc
    12361 tctctttgct gaacagtccc agtatttttc ccgttccggg aactaccagt gcatctatct
    12421 ttcctttctc catgtcaatt tccacgtcgt aaacaactcc gagccttctg ccgtcattta
    12481 tgtttattac atccttctgt ttaagttccg atatcttgac cattcacacc cctcccatca
    12541 tacttgtttg cttatgctag tatataaagc ggaagcaaaa aatagaacaa aaaacctcaa
    12601 ttgaaccaaa gcattcaaat gaggtttcta tgaatcatat catatgtgtt ttctcatatg
    12661 tccaagggct gtcttttcca gccgggagac ctgagcctgg gagattccta tttcttccgc
    12721 aacctccatt tgtgtccttc cctcaaaaaa cctcagatta agtataagct tttccctgtc
    12781 gttgagcttt ttcattgctt ccttgagagc aatgctttcc agccaattat cgtcggtgtt
    12841 cctgtcatcc gatacttgat ccattacata tattgcatca ccgctgtcgt gataaatagg
    12901 ttcaaaaaga gataccggat cttgaattgc atccagcgcg aataccactt cttccaccgg
    12961 aagtttaagc tcatttgcaa tttccgatac ggtaggttcc tttgaattct tgccgataag
    13021 catatctctt acctgaaggg ctttataggc aatatctctc aaggatctgc tgactctgat
    13081 ggagttgttg tccctcaggt atcttcttat ttctccaatt atcatgggta ctgcataggt
    13141 agaaaatttg acgttctgac ccaggtcaaa attgtctatt gctttaataa gacctataca
    13201 gcctacctgg aaaaggtcat caacatattc ccctctgttg ttaaatcttt gaatgacgct
    13261 taaaacaagc cttagatttc cttttatgaa ttcttccctt gaggattggt ccccctgctg
    13321 catttttttc agcagttcaa ccattttttc attagagaga acgggcagtt ttgaagtatt
    13381 tacaccgcag atttctactt tgttaagcat agcacaagca cacccttttc ttaatgttga
    13441 catattaatt attaccatag gccaacttta ttattcaccg atgagcttgt accaatttgt
    13501 tttaaacaga acaattttgt attagaagaa taatgcgaca gggacaggag aaacgtcccc
    13561 gtgtcctcac gtcgcattct tcataccatc ctgctgattt ctttctttag ccgctttata
    13621 atcctcttct ccagccttga tatgtatgac tgcgatatcc caagcatatc cgcaacctcc
    13681 ttctgggttt tctccactcc gtcattaagg ccgaacctta attccattat cctcttctct
    13741 ctttcggaca gctttctcag ggcaatttcg agaagctgct tgtcaatctc atcttcaaga
    13801 tacctataga ttatatcatt atccgtgccg agaatgtcag aaagcagcag ctcatttcca
    13861 tcccagtcaa tattcagagg ttcgtcaaag gatatttcgg tttttgtcct gttgtttctg
    13921 cgaagataca taagtatctc attttctata caacgggagg cataagttgc aagctttatt
    13981 ttcttttcag gatcaaaagt gtttacagcc ttaatcagcc ctattgttcc gatagatacc
    14041 aaatcctcaa ctccgactcc ggtattttca aactttcttg caatatatac cacaagcctg
    14101 agattccttt ctattagaac ggccttgact acttcttcat cacccttgag gcgcgataaa
    14161 agaaagtttt cttcctcgtt ttccaggggg ggcggcaaag cttcgcttcc gcctatataa
    14221 tgaacccaag tttctttata tactttaaaa aactgtaaga ttctgattat cagcatgcgt
    14281 tttatcaagc tcaatcttcc ggctgtcttt ttcatttctt aacctccatt ttgtattgtt
    14341 gtctgcaaat ccttgggcgc atcttatcta tccttcaaca tctcgggatg tatcagggcg
    14401 ccgtattccc catctccgga gagatttctc ctgtatatgg caattattat atcggattgt
    14461 attcttctgt ccttgtcgaa tattattacc tcatcaggct tgaagcctac gagcataccg
    14521 ttctctctgc ccaatgacct gaagggtatt attctgaatc ttgaagccca cttggaaatg
    14581 gtcattattt ccgctattgc atttaaatca ttttccccct cttgttcaaa aatcttctgt
    14641 acatcatcag gcagcaattc ttttattgta tcgtattccg ttatcataac aggagtattg
    14701 gtaagaggat ccttcagtga gtttcccgta tctacaagag catccatgca tagctgcttc
    14761 ccgtcaaagc gcatctgcac cttgagaatt attttttctc tttttatcct gtgatgtatg
    14821 taatcccagc tgtacctgat aataaaatat gcagcggcaa cagccgcaat aagtattttt
    14881 acgggaaaat ccctgatgaa agaaatcccg ttgctggtca ggttcaggcc gtttataaag
    14941 tagaataaac caaaggcggc cccgccgaaa ataaaggaaa taatatagaa aatacccgtc
    15001 agcttcagca aaaggtgaaa atatgccgga gtgtatgcga ctatcacaat aagcagtgaa
    15061 aaaagtattt tcatgtaaaa tgtaaaaaga tacccgtatc cggggaaata ggaaagtact
    15121 gcataaaccg ctcccagcaa agaagcaatc agaagcttca cctttgaatg cctgtatctc
    15181 gcaagccgtg ccgtactcca taaaatcaaa taattcatta ttaagttttc cagcaatatg
    15241 atatccgcat aaacataagt ttccaacttc attcccttat ctttcatatg caatatattt
    15301 caggacaggc ttctgacaac atataatccg gatatcctaa aacatattcc ttgattcata
    15361 gaatacgcca agattaccag gtatgataaa tcaactgtta tccggcgtat tctatatata
    15421 tcgcttatga taattatata aaaaacttca tgaaatacct gtcattttta gtagtcgaaa
    15481 taaattatat tatcatattt atcgacagca gccttcaatg ggcgttaggc caattacaac
    15541 taatatatat attcaaaagg caacaaaaaa agaaccaaaa attggttctt ttcagtttgc
    15601 agacaaagct taaaatagga acagcaatgt atagtcagaa catacttata caaaaattgt
    15661 taatggccct tatgaagctc gacttagaaa caaccgtatg cgaggcacgg aggccgagca
    15721 agctgtcacg gtcacggacg acctttgaca gcgctaggtt tttcttagtt gagcggaatt
    15781 agagccatca atttttgtat acctatgcga gactatacat tgctgttctt aaatttgagg
    15841 tttttcatca gtctaaaaag aaccaaaatt aattggttct ttataattgc tcctattccc
    15901 cgaaacctcg aagtacgctt acttatatct gttcctcaag aaagtcggta tatctatatc
    15961 ttcattacca tagttggata ttttctgccc gtcccctttt tcctccggga ggccggcaac
    16021 ttcgatcctt ctttcaaaga acctttgggc accttcttca aagcctgttg caataacggt
    16081 aatcctgatt tcatcctgca tgctctcatc gattaccgca ccgaaaataa tatttgcatc
    16141 cggatccgcc gactggaaca caagatccgc agcttcattt acttcaaata ttccaaggtc
    16201 tgctccgccg gtaatattga gaagcacact ttttgcccct tcaatgcagg tttccaaaag
    16261 cgggctgtgt attgcctgtt tcactgcctc ggaacttctg ttttccccgt tggcctttcc
    16321 tattcccata tgggcatagc ccttgtcgag cataatggtc tttacatctg caaagtccag
    16381 atttaccagg ccggggacgg caataaggtc ggaaataccc tgaacaccct gcctcaaaac
    16441 atcatccgct atcctgaagg cctccattat agacgctttc ttatctacaa tctgaagcag
    16501 cctgtcattt ggaattgtaa caagagtatc aaccttctcc ttcaattcag tcaggccttt
    16561 ttcggcttgc attttcctta ctttgccttc aaaaccgaaa ggccttgtga ctactcctac
    16621 cgtaagtatt cccagttctc tggcaatctg ggcaactaaa ggcgcagctc ctgtgccggt
    16681 gccgcctccc atacctgccg taacaaatat catgtccgcc ccctcgagaa ctttctttat
    16741 atcgtcataa ctttcttcgg ctgctctctt gccgatgtcc ggatttgctc cggcacccag
    16801 cccctttgta agtttctcac cgatttgtat tttatttgga gccagcgaca tatgtaaagc
    16861 ctgtttatcc gtatttatgg catagaactc tactcccttt aagcctgcat taatcattct
    16921 atttattgca ttgtttccgg ctccgccaac cccaacaacc ttaatctgcg caatagaatt
    16981 aatatcaaca tcaaattcaa gcacaatata ccctccctat ctacaactat tatatatgcg
    17041 aaaaattgta taccaagttg attcatctta ataagttcaa catatctctt gattttcctt
    17101 tatttttttt aacttttatg gttatttttt tttgtgctcc ataagtacat atcttcttat
    17161 tattgcaaaa ttctggaaca gcctgactcc gaaggcaaat atcgctgcgt agtatatagg
    17221 cactccaagc tggtctccta tatatgtaag tatggcagcc aatactgcat tgccgaaaaa
    17281 tccggacata aatatctcaa tattgaaggt cttttctata ctggacctta ttcctccgaa
    17341 aaccgaatcc aaagccgcaa gaattgctac cgacatgtag cttgaatatt gaatcggtat
    17401 ggtaatcggc gaaagtattc caatgacaat tcccagcaat agtcctataa ttggtataat
    17461 cattatgctt caccgccttc agcttttctt tgatacttca tcttacccag gttattttgg
    17521 ccatttatga caagcttttc tactttttga atctccacct gtataccggt atattccatg
    17581 taaagctcta tatagctatc cggagccagg gcagaggctt caagggcctt tggatcacct
    17641 attgccctta tgataaaagg agctgcatgc cttttaccgt caatatttat cgttgggcct
    17701 ccgcatctta tgttggtagt ggcggttact ctttctccgt ttattgatat tgcttctgct
    17761 cctgccactc taagctcatt gactatttca aggatatcta tatcgtgtac caggtaccat
    17821 gcaatgtctt ccccctcagc aagctcctgc tggctgtcat tcagggtcat tatgattccc
    17881 ggcccctgaa cggcataaaa gcctgcataa ttcctggcct tttccagctc attctgcatg
    17941 gttccatagg cactccccgt ttcttccacg cttgtcttat actcaaccaa tctcattgca
    18001 tgttcgctga tggactcctt aagcagcttg ttttccattt ccacattgct gatctcattc
    18061 tgcaaatcca ggagctttgg tattgtaatg ataccctgat aggtaatatt ggattttagc
    18121 tgcactgtaa caataaagcc aataatcacc gccagtatga acaaataata tatatatgcc
    18181 tttctattca tattattggg attctccctc tttaacaact tttgcatatt tcctctcaat
    18241 aactccgttg tatttctgaa ttttaatcag ctgctcctgc gtaatcgtca cagccactcc
    18301 actattctca agcttttctg cgatcccttc cctcagcctc aatgccgctt ccagagtctt
    18361 tgggtctccg atggctttaa acacaaaggg aggggcaaag gagacagtgt tgatattaat
    18421 gtgtattccg cctatctgac gtatttccga agtggatatg attctttgtt catttatgga
    18481 tactgcttcg gcaccggcag cattaagttc atttaaaagc tccaaaagca ttgaatatga
    18541 aatcagggga taattcacct tttcgctgaa tttcaaatcg tctacgacaa cggtaatccc
    18601 cgggccttca acatcctcta tgcccgccag tatccttgcc tgatccagtt cttccttcaa
    18661 tatctttgcg tataaactgc cttgagatgc agtatcttca aactccttga tctttgattc
    18721 ataatcgttc ttaacattct gcagcttgac attttcttct ctgacttttt taagctgtgc
    18781 cgccagttcc cttgccctgt attcagacac agggccgact ccctgcttta cagtcctgaa
    18841 ctggattgaa accatgatac ccagcaccat acatacaact gccactgcaa tttgatattt
    18901 aatatttttc attatccggt cctcctatta tccccactgt tcactgctcc tgtttaaaaa
    18961 caggattgcc attatggctc atctctatag ttcctttaag attctgcttc attacatctt
    19021 caattaccga cctggcaaaa ttaatcttgt agtccatgtt atcggcagaa cccagattcg
    19081 cttttatgcc ttccttaatt atcagcctga tatttgataa gtccgagata tcaatctctg
    19141 aaaccagatt cagcattccg gcattcttta tcaccttgat aagctgagta gttgttaaaa
    19201 actgttcttc atttttaaca ttcagaaact ctccgacctt gaaggtctca aactgcaatc
    19261 ccttaaccag cggtaaatcg gtacgcttaa ttgcagggtt gatttccagt atcatgcctt
    19321 cctcgtcgat gttgagataa gaccctacat aaggtacata agccataata cctctttcta
    19381 ttatatcaat aacaacctta tcgggaagaa ctctattaat cttaatcatt ttgacgtaag
    19441 gattctgaaa aatactcttc attgagtttc tcttatttat tctgaatata ttctcgccgt
    19501 aatatattga tgaaagcttt attatttctt ctcttgttac gttattattc ccatttacaa
    19561 ccagatactt tatctgaaaa aagctggaag acagaaaaaa cgcagtaaat atgcagatta
    19621 tgaataataa aaccacatat ttgaatcttc ccctcatgtt ttcactccta aaactacaat
    19681 atgtgcttgt cccaagaaaa cttaaaaata acactgcatg cagttccgta tgcatacagg
    19741 ttaatattga aaatgctata ctctgtgtat gtcagcacct aattttgcca gtttattttc
    19801 tagtttatca tatcctctgt ctatatgcaa tttaacattg tccactacag tagtcccttc
    19861 tgctgcaagc cccgcaagca ccaatgcggc tccgcccctc aaatcgccgg ctttaacctc
    19921 ggtgccggta agctttttta ccccccttac aacggcagtc cttccgtcca cctttatatt
    19981 tgctcccatt ttcataagct cttcaacatg cttgtatctg ctttcaaata ccgtctcaat
    20041 gaatatgctt gtgccctttg ctattgtcat tacggccatc atctgagcct gcatatccgt
    20101 tgcaaatccc gggtatggaa gagtctttat cacctcaacc gcctttggtt ttttgctgca
    20161 cgttatttga atgctgttct gatactcggt aatactgcat ccgctttccg aaagctttga
    20221 cgataccaat ttcaaatgat caattattac gtcatttaaa acaatatttc cacccgttat
    20281 ggcggcagct gccataagtg ttcctgcaac tatcctgtca ggtatgacgg tatgttccac
    20341 gtcgtgaaat tcctttaccc cctcaatctt cacagtactg gttcccgctc ctgatattct
    20401 ggctcccatt ccgttgagaa aattttgcag gtcaacaatc tccggctcct tggcagcatt
    20461 ctgtattata gttacccctt cggctgttgt agccgcaagc attatgtttt cagtggcgcc
    20521 tacacttggg taatccaggt ttatctccac acctttaagc ctgtcgcact cacagttaat
    20581 atatccgtga gcttccgtga ttttagtgcc cattctcctt aggcccgaca aatgaatatc
    20641 aatggggcgc ggccctattt cgcagcctcc ggggtaactg atttttacat gcccgtgacg
    20701 tgcaagcatt gctcccatta gaataattga ggatctcatt tcccgtaccg gtttttccgg
    20761 tataatagta ttcaactggc tattggattt tacgactaat gtattgttct caaaaaaaca
    20821 cctgcagcct attgtccgaa gaagtccgat cagcaaatca acgtccttaa gctccggaac
    20881 attatggata accgattctt tcccacttat tatggtagcg gcaagtacgg gcaaaataga
    20941 gttcttggat ccgtcgaccc ttacatctcc ttcaagtctg cgcccgccga ttatcacgta
    21001 tttatccatc aacgatttac acccccggga agccattatt cttcaatcat attaatatat
    21061 tatgcttcac ttcccaaggt gttactgcaa ttgcaacggg ccaggcagta tcaagccttg
    21121 caaatgctag gctttcttat tttgaacaag cctttcggca gcttttgcta ttgaataagc
    21181 cgcatcagtt ctgcccagtt tcttgctttt tgctgccatt tgacttactt tttccggctt
    21241 gttcaacata cctgcaatct cattatacag tatatcagcg tccaagtccc tgtcctgaat
    21301 aacaattgca gccccgttct tctccagtgc ccttgcatta tactcctgat gattgccggc
    21361 agcataagga taaggtatca gtattgaggg tttgcccaaa gcagttatct ccgacagggt
    21421 gctggctccg cacctgctta ttattatatc gcttgcggtc agggcatagg gcatattgta
    21481 aacatagggc attaccctga gatatcctat atccagaggg atgttttcat tatcgtattt
    21541 tttaagtatg tcatcatatt gagtctctcc ggtcatatgc agcatttgaa agcttttcga
    21601 ctcaacgcac tttttggcaa gcagcatcac gctttcattc agtttcctgg ctcccctgct
    21661 tcccccgaca acaagaacta ccggcagttc ccggctaaaa ccaaattcct taaggccttc
    21721 ctctttgctt acactcagta tggtgctcct tatcgggttc ccggttacaa tcaccttgtc
    21781 cttcctggga aagtactctt tagcttccgg aaaattgact gcaataatgt caacaagcct
    21841 tgccagaatc ttattggtga ttcccgggaa ggcgttttgt tcatgtatca gggtcggtat
    21901 acccataagt gcggcagcaa gcagcaccga accgcttaca tatcccccgg tgccaattac
    21961 tatatccggc ctgaaatcct ttattatctt agatacctta aaaaggccgg tgaaggattc
    22021 cgccaccgct gtgacattcc caaagctgaa tttccgcagg aagcctctta cctttatttt
    22081 taccagctta aagccttcct tgggaacaat gctgctttcc aatcccctat cggttcctat
    22141 gaacagtatt tcagaatcag tatacagatt tttaatttct ttggcgatgg aaactgcagg
    22201 atatatatgt ccccccgtac cacccccgga aagtatggct ctcattggat tcaactcctg
    22261 cctgttttga ctgtttttga gatattcaat aaaattccca tgttcaccat aacaaacagc
    22321 agcgacgatc ctcccgaact gataaaggga agcgaaatcc ccgtagtagg tatggatact
    22381 gtcgccacag ctatgttaag agtagcctgt attgctatca tacatgtaat tccggtggca
    22441 agcaggcaac cgaaggtatc gggacaggcc aaagctatcc tcaaacccct ccatataagc
    22501 atgagaaaca atatcactat tgttgcggtt cctatgaatc ccagttcctc cccgataatt
    22561 gcaaatatga agtcattttg cggctccggt atgtaaaaaa acttctgacg gctgtttcca
    22621 agccccaatc cgaatattcc ccccgagccg agggcataaa gggactgtat tgcctgataa
    22681 cctttattca gcggatcagc ccagggattc atataagagg tgacccgcgc cagcatataa
    22741 ggctttttca taattatata gcctactgtc ggcaggactg gtatcagcca gcctatcagg
    22801 taaatgattt tcgtccctgc tgcaaaaagc attgcaaagg ttatcatcaa aatgcttccc
    22861 gccatactca agtttggttc aagtattatc agcacaaaat atatggccgc caaaatcacg
    22921 tatggcagga ctccatgctt gaagctcctg attttctcct tcttattgct tatgcttttt
    22981 gccatgaaaa taatcagtgc aaactttgca aactcagatg gctgcatcgt ctgtgacccg
    23041 atctttatcc acctggtggc ttcattggcg gtgtatccga ttccgggtat gaatactgcg
    23101 attaagaggg ctatgctcaa tagcaccagc gggccggcaa atctccccag aatcctgtaa
    23161 tcaaacctgc tcataaacat catagccagg atgcctaata tggcaaaaaa accttgagac
    23221 ttcagatagt ggtacctgtc gttatacttc ataaaggagg aaattgaact ggcggaaaaa
    23281 accatagcca cacctatagc caataatatc aataccgtta tcagtagtgt aaagtcaata
    23341 ttgttcttac tccttgcagg catcaaaagc cctcctaaga taatataatt ggactgccat
    23401 cggccgcccc tgctctaaag acttgcttaa ttttgcgcct ggtatctctg atcccgtatc
    23461 cgaagttccc ttacatattc cttaaagagc ctgcctcttt cctcataatt gctgaacata
    23521 tcccaactgg cacaggccgg cgaaagcagc acgcagtcgc ccggttcagc aagtgaatgg
    23581 catttcatca cagcctccct caggtccctg gctttatgcg tattcataaa cccatgccta
    23641 tgggctgttt cctccagctt atcggcagtt tttcctatca gcaccatatg cctgaccaca
    23701 tccccaaagg cctcgatgaa gtcatcatat tcgcttcctt tgtcatagcc ccctgctatt
    23761 attatagtag gccgcttcat tgcctcaacg gccttgatgg aagcatctgt gttggtacct
    23821 tttgaatcat tataataaat tactccgtca agttcctcaa catattctat cctgtgttca
    23881 acacccctga agctttccag ggtatcggca attactgaag gggccacccc cgcgcaatat
    23941 gccatgagag ctgcagctaa agcgttttcc aaattgtggt ttccgggtat atagatttta
    24001 tccttgtgta ttattgtttc tttattatcc gccttgatta ctatataatc tttttccata
    24061 tataccccct ggggcacctc tttctttctg ctgaaaaaaa gcaccttgct ttttggcctg
    24121 tctgccagct tggaagtctc cgggtcatcc agattcagta tggtataatc cttttcatcc
    24181 tggtttgcaa aaaccctgca tttggcatct atatagtttt ccatgttttt atgcctatcc
    24241 aaatggtcgg gtgtaatatt caatattgca gaaatattcg gcttaaaggc aatcgtggat
    24301 tccaactgga agctgcttac ctccagcaca aaaatatccg gcctctttgc tttatccacc
    24361 tcatttatca tagcagaacc gatattgccg gcaacatagg tcttcctccc gcttgcccgc
    24421 attatttccc caagaaggga cgtagtagtg gtcttgccat ttgttccggt tattgcagca
    24481 atgggtgtgt ttgtcaggcg gaaggacagc tcaatctcac ttatgacttc tttgcccata
    24541 gcctttgccc tctgcaccgg cggaatatcc aaaggaacac cggggcttat aacaacaaaa
    24601 tcgcaatcct cagccagcct tgccggatgt cccccggcca ccagctttat gggcagttcc
    24661 tggatttccg agattacctc tttaagctcc tcaacctttt tgaaatcatt taatacaata
    24721 ttcgcaccca accggctcaa ctgtttaaca acaggtactc ccgtaaccgc agcccctata
    24781 accagaacct ttttcccctt tatctgcata agtactttcc tccgcaatac cttttactcg
    24841 taacccttcc ccagagccca gccctaatcc tcgcatcccg caacctcgca ccccgaaacc
    24901 tgttttacga taatcccaat attcctatca agcttaatat tattgaagat ataatgaaaa
    24961 ccgctactat tttcgattcc gcccagccgc ttagttcaaa atgatggtgc aggggcgcca
    25021 ttttaaagaa ccttttccct ccggtcatct tgaaataagc cacctgtatt attaccgaca
    25081 gcccttccag aacataaatc gcacctatta tgagaatgaa taagggaagc ctggttatga
    25141 cagcaagaga agctatagct cctcccaagg ctaatgaacc tgtatctccc ataaatacct
    25201 gtgcaggatg agaattaaat cttagaaatc caagcagtgc acctatcata gcccccgaaa
    25261 acaccagcag tccggtattt tttgaagtca gtgctattac cgagaaaaat ccaagcacca
    25321 caactgtggt tccccctgcc aggccgtcca ggccatctgt aaggtttacg caatttaccg
    25381 tagatatcag tacaaagact gtaaatggta tgtacaatat tcccatatca ataaactttc
    25441 ccgtaaaagg taccagtacc tctgttccaa cctgtgatat ccccgaagca acaacagcca
    25501 aaaaaacaga aaccagaagc tgcagtatta tcttctgata agccttcagg cccagggatc
    25561 ttctgttttt tatcttaaga aaatcatcaa taaacccgat tataccaaaa agcatagtgg
    25621 taataagcac caaaagcata tctttatcct tggagaaagc cagtgacaca attactgcag
    25681 gtactgcaat tattatacct cccattgcag gggttcccgt ctttttcaaa tggctctgag
    25741 gcccgtcatc cctgacaatc tgcccgaact tcattctcgt aagcacaggt atgaataccg
    25801 gccctgcaac caatgccagt acaaaagcag agattatgct tatgatatat atatcatatt
    25861 caccggatac caaattgtac attaaagctt atcccctctc gcgtaaatag tcaactatgt
    25921 attccatttt cattcccctg gagcctttta caaggacggt atctcccggc cttattatat
    25981 aatcaaggga tgcggcagca gcttttatgt ctgaaaaggc atatacattg cagttcattc
    26041 ccgaggccgc tgcccctctt ttaatatctt cggagaattc tcccacagct attatggcat
    26101 ctgctccgga ggaagcggca taggctcctg ttttacagtg ttcctcagaa gaaagattac
    26161 ccagctcaag catgtccccc agtatgcata tgcccctgcc tttcgacttc attgtgcgca
    26221 atacgtcaat tgccactttc atggattccg gatttgcatt atacacatca ttaatgagct
    26281 ttaccccgct gccgagaact atgatttcca tacgcatttc cgacggtttg aagctatcca
    26341 atcccttttg tatttcctct gccttcatgc caaaaagcct tgcaacggca atggcggaca
    26401 aggcattata tacattatgt attcccggaa gcaagactct aaaattcgac tgtccatcca
    26461 aatccactga gaattctatt ccagcttcac ccttttcctc tatcccatag gcgaataaat
    26521 cattgccttt tttcaggcca tacttaataa ccctgaactt atccgaacag aagtcacgga
    26581 gcatgtcgtt atctccgtta ataacagctg tgctgtcact attaaaatat gaaaatatct
    26641 ccagctttgc tctcagtatg ttctcccttg aacctaactt ctcgatatgg gtaaggccta
    26701 cattggaaat tactccaata ccgggccttg cgatatccga cagcaggctg atttctccga
    26761 ggctgctcat tcccatctcc agaaccgcaa tctgatggct gtattgaagc ttgagaagcg
    26821 tcaggggcaa accgatggca ttgtttaaat ttccttccgt ctttaataca ttgaatcttt
    26881 gggtcagcac cgatgctatc atgtccttgg ttgtcgtttt ccctgagctg ccggttattg
    26941 ccacaattgg tatattgaac ttgcttctgt agtaagctgc aatgtcctgc agagctttca
    27001 gagtgtcttc caccagtatg tacggtattt tgcatctctc cggcagcctt tgggttactg
    27061 ccaataccgc gcccttttca atggccatgt cgataaaatc gtggccgtca aagttctcac
    27121 ccttaagcgc aataaacata tccccgggat ttatttttct gctgtcaatg cttacacccg
    27181 agatctcaga ttcttcttca aaattcatga gtactccatg tgcagctctt gctatttccc
    27241 ccatacttaa ggcttccatc atacttcctc cctcagaagc tcctctacaa tatctctctc
    27301 atcaaaggga atagtcctgt ctctcaatat ctgataagtc tcatgcccct ttcctgccag
    27361 caaaactata tcgtcgggtt tcgccgtggc aagagcaaat tttattgcat ctcttctgtt
    27421 ttctatacaa atatagtcac aacctgtgga ttttattcca gcttctatct gcatgattat
    27481 atccatgggc tcttccgttc ttggattatc cgaagtaatt atgcagtaat ccgacatcct
    27541 tccggcgata tttcccatta tgggcctctt ctccttgtcc ctgtctccgc cgcagccgaa
    27601 cagggttatt attctgcccc tggcatactc tttcactgaa gccaggatgt tctgaagccc
    27661 gtcgggagtg tgggcataat caataattac cgtataaggc gtatttgtct ttataatctc
    27721 cgacctgccc ggaacatttt cgactctgct cagtccttcc cggattacct ccatgtccaa
    27781 cccctccgca tatgcagccg aggcagcagc caaggcattg tatacgctga actttccggg
    27841 tatactgatg ttcagttctg cctcatattc gggagtaata acagtaaagc ccactccttt
    27901 tgaagttata tcaatatttt ttgccattat gtccgccgca ttgtcaatac cgtaggtaat
    27961 cagctttttc ccgttgttct tcaactcggc ggctattatt ttcccgtgct catcatcaat
    28021 gtttattata cttgtaccgc ttaccatata aaagagcttt ttctttgcat ttccgtaatt
    28081 ttcaatggtt ttatgaaaat ccaaatgatc ctgggtcaga tttgtgaata ttcctattct
    28141 gaaattgctg ccttccaccc ttccaagctc caatgaatgg gaggatacct ccattacagc
    28201 ataatcgaca tcgctgtccg ccatctccct gaaaaggcac tggagatcca aggcttcagg
    28261 cgtagtcctt gaagaaatat atatcttatc gccgatctgg gttgaaattg tacccagaag
    28321 cccaattttt tgcttgtttg cttccattat tgattttatc agatacgtgg tggtggtttt
    28381 tccgttggtt cccgttatac ctatcagctt aagcttttgt gagggatttc cgtagaaatt
    28441 ggatgcaagc atcggcataa cagcccttga gttttccacc tttaccaccg ccgctcccgg
    28501 aacttcgacg tccttctcca caataaaagc ggcagccccc ttctgcagcg catcatttat
    28561 ataactgtgc ccgtcatact tatagccgct gacacagata aacaggtcac ccttctttgc
    28621 ctttctggaa tcatatatta ttccgtttat ttctatattc aaatcccctt tgaaagatac
    28681 agtatcaaca cctgttaaca attcctccag cttcatattt atcctccttc atatacagct
    28741 attgttgaca tgttttggca tctatatgct gaatgcgtaa tcatcagttg taaatcactt
    28801 tattattatc catcacattt tacaaaaagg caaccaaaat taaagggttt ctttaatttc
    28861 gttgcctggc aataaaacct cagatatatg cttaatacat aatgctatta cttcattgta
    28921 cgggtttagc attcaaaatt acaatagacc cttcattaat atctgctccg ggcttcggag
    28981 tctgatcaac tattgttgat tccatgttat gctcaaattc tgcttccaca ctgtatttga
    29041 gcttgctctt ttcaagttcc tttatggcat tcttaagggc catgtttctt acttccggca
    29101 cctttacaag gggtctctgc ataagctcct tttcttctgc agtatattgg ggtttgacat
    29161 ccatgtatct cagagtgtca tatattatgc tcctggctac aggtgctgca attacaccgc
    29221 catagtactg gcctgctccg ggctcgtcta tgaccaccag cacagatact ttggggtcgt
    29281 tggcaggagc aaaaccgcag aaggatgaaa caaatttgcc tgatacatat attccccctt
    29341 cggctttttg agctgtaccg gtcttccctg caaccttata tcccggaata taagaggatg
    29401 cagtgggcgg tatcgcaaat tccagaatct gcctcattgt cgccgaggtt tcccttgata
    29461 ttacctgtct gactgcccga ggctggaatt catggactgt cgtttcttca ccatcgatga
    29521 ctttcttggc aattctgggc tccataagca taccgtcatt ggcaaccgca gagaaggcgg
    29581 ttatcaattg aataggcgta acggatattc cttgtccgaa ggaattattt gcaagctcaa
    29641 ccgggccgac cccgcccaat tctcttacaa tacctcttgc ttctcccgga agcttgatat
    29701 ttgttaccga accaaagcca aaggctttta tgtatttata cagcttttcc tttcccattc
    29761 tttgccctac ttctacaaat acaggattgc aggaattctt gacagcctct gcaaagcttt
    29821 ggttcccgtg aggcttgtag cttctccagc atttcatctt cttccctgcc accgttatgt
    29881 gccctatgca gttgaaatga tcctccggtt taacaacgtt ttcttccaat cctgcggaag
    29941 aggttattac cttgaaagtg gaaccgggtt cataggaatc cgaaatagca ggatttctcc
    30001 atatcctata aagtacatcc agctgctctt tctcgctcat ggtgctccat ttatccgcaa
    30061 ggtcctccgg aaccttccgg ggatcattgt tatcaaaatc gggtttggaa gtcatagcaa
    30121 gaatgtcccc tgtcttagga tccatcacta ttgccgtaac ccgctttgcc ttattctcaa
    30181 taagtgcgtt ctcaacaacc ttctcagcga aatgctgtat tacttcatca attgtcagca
    30241 caagattcag gccgtcctga ggctcgtaat acttggtgtc gcttcccgga agcatctctc
    30301 cgtgggcatc tgtcattaca atattccttc cgggaaatcc gttcaggtac ttttcatatg
    30361 taagctctac cccatcctgt ccctggttgt cagggttggt aaaacccaat acataggaag
    30421 cgaaattgct ttgagggtaa aatctcttgc tgtcgtcatt aaatacaatt ccctgcagtt
    30481 ccttttccct taaaagctta accttttcgg cgtccacctt tttcttgatg agtaccgtgt
    30541 ccttattggc agttaccttt tgagccaaag aagccttatc catccccaga atttccgaca
    30601 gggcatttgt aaccgcatct ttatccttga ccatggcagg ccttacatat atttcatatg
    30661 ctgttgcgct gacagccagc tttttgaagt ttctatcgta tattattccc cttttggacc
    30721 ttatcgggat atcccttgtc cactgttcca ccgctttctg ctggtattcg gcactgttta
    30781 ctatctgtat aaaagccagt cttcccagga gtgccgcgta aataacaaaa aaagcaaaga
    30841 agataattac aattcttctt tttatagtca ctttaatgct ggacaaatta atacaccacc
    30901 ttcagaaaca acctcaagct aatctaatac ttttaggata ttatttatca cagctttggt
    30961 atatgctatg tatttattct cctgtaaatc gtcaatgttg tagaagtcac gagcctcatt
    31021 gccggcaggc tgtgccggta tctcgacatt tacataagca atctggtttt gaatatcagg
    31081 atactgcatt cccagctctt caattgcctt tttttcaaga gtctcaaggc tgattgactt
    31141 cataagctgc acattcaaat cagtattttc cttaaccaga gcactgtatt ccttttccag
    31201 gttttttata ttgtagttta tctccgccat ataagcatag cgtgatataa taaaaatgct
    31261 cagcccaaaa acaaacaata tactcatcag atgagtcttg tgtttttctt tcttatgaac
    31321 cttctttcgg gggctcgcag ctatttcccg gacttgctcc atttcaagct gctcatatct
    31381 gctgtaatca tatgccgtat tgtctgctct taacattatt attcccccct aacccctttt
    31441 agaacttact ctttcggccg ctctaagctt agcgcttttc gacctggtat tagtcttcag
    31501 ttcctccact ccggctgtca cgggtttttt tgtaatcact ctgagaagag gctcttttcc
    31561 gcaggcacat accggcagct gaggcgggca tgtgcacggc ttttccatat tgttaaatat
    31621 cttttttact atcctgtcct caagggaatg aaaggttatt acaaccagcc ttcctcccgg
    31681 cttcaaaagc cttgctgcgt ttgtaactgc ctcttccaaa acctgaagct cgtcattcac
    31741 agctattctc aaggcctgaa atgtcctttt ggcgggatgt cccccatccc tgcgggccgc
    31801 cgccggaatt gccctttgta taatatccgc cagcttaaag gttgtatcta tcctgctgtt
    31861 ttttctttct tccgttataa acttcgcaat cctgaccgcc catttttctt ccccataatc
    31921 cctgagtata cggataagct cctgttccga actattattc actatatccg aagcattaag
    31981 gccgttttcc gtatccatcc tcatatccag ggggccgtcc tgcatgtagc tgaagcctct
    32041 ttccttctca tccaactggt gggaggatac ccctatgtca aggagtattc cgtcaacttc
    32101 cttaaatcct ttttgcaggg ctatggtttt tatattcctg aagttgtcac ggacaattat
    32161 tacattgtcc ttatatgctt caaggcgcct cgttgcagca tcaattgcat tgctgtcctg
    32221 atcaatgccg atcagcaccc ctccgggaac tatccttttt gctatttccg aagagtgccc
    32281 cgcccctcca agggtaccgt caatatatat ccctcccggc ttgggatcaa ggtattccaa
    32341 tgtttcattc aacagcacgg gaatatgttc gaaatccaat actgccatct gcttcacatg
    32401 ccttttcgct atattccgag ctgtgacatt ttttcagcca catcctcata gctaatattt
    32461 gcatcgttgt taaatttatt ccattcttcc ttgctccata tttctattcg ggttgagaca
    32521 ccaataatta caagctcttt atctatattg gcatgctcct taaggtaatt tggtatcacc
    32581 atcctgccct gcttgtccag gtcacattcg caggcccccg caaaaaagaa tcttacaaag
    32641 gctcttgcat ctttgtttgt cataggcagg ctcttaagct tatcctccac tatgctccaa
    32701 tcccgggacg agtatatgaa aaggcagttg tcaagaccct tggtcataac aaagctgtcc
    32761 ccaagctttt ccctgaattt tgaaggcatg atgactctgt tcttcgggtc cagggtatga
    32821 ttgtattcgc ctataaacat atactcacca cctttataac aagatttcaa taagagtttt
    32881 gcatacttgc aaaaacctac ccgagcgttt caacggggca gagatatgtt catcttctgc
    32941 tgaacaacga aagcgttacc tgccgcttgt atagtattgg agaaacttat tgccccgtta
    33001 tattcacatt ataccacttt gctccacttt agtccacttc ttgacatatt ctatacaaaa
    33061 ataaaaattc ctcccttttt taaaaaaata ggagaaattt taaaaaaatg atgcaaaaga
    33121 cctatgcttt tgcatcgtta ttattcttca gcaatatcct cagtgtcctc cccggcctta
    33181 tccttattct tttttctccg gtaaacaatc aaggcagtga aggccgcaat tataaccaca
    33241 ctgataatct gagcctgcct cattcccata aacatcaggc tgtccgttct aaggccttca
    33301 atccagaacc ttcccacaga atacagtatc ccgtacagca ggaatacttc tccctctgcc
    33361 ttcttctttt ttctgtatat cagcaggaat ataaaaacac aaatattcca caaggattca
    33421 tacaaaaagg tcggatgcac ctttactccg tccaccatta tgccccaggg caaatccgtc
    33481 ggctctccgt gagcctcctg atttacaaag ttgccccacc ttcctatggc ctggcccagt
    33541 attatgctcg gtgcagtgat atctgcagta ttccagaaag atatcttttt cacccttgtg
    33601 aatacatacc ctgtaataac ggcagctatc aaagctccat gtattgccag tccccctcct
    33661 cttatgttta ttattttcat aatgtcacct ttataatagc tccactcgaa tatgacataa
    33721 tagagccttg caccgattac agccgccgga acgcaccata acgcaaggtc gattatatac
    33781 tcgggatcgt acccaagcct ctttgcttca taatatgcca gcagtatacc tgcaattatt
    33841 cctgccgaaa gcagtatgcc ataccatctt attgctatcc cgtatatttc aaaagctatt
    33901 ggatccatag cctctcctcc tgttttgctt atgctgcata tatgcagtat tatcctttac
    33961 tgtttgatta taaatatacc gtgattgaat tgtcaagtaa taaggggctg gagacacggg
    34021 gacgtttctc cagcccctca ttcttttcct aatccgaatt aggaaggtca agtactccgt
    34081 tgacggtttc tataaccgac atgaaattct tgccctgcat ataatcatca tatgccagat
    34141 acagagccca tatcaaatgc cacatatccc cgtcggcata tactattttg agccccaaat
    34201 cattcaaggc ctctttcctg ttgatgcaat agccgtggga gaaatatttc tcagtcatgg
    34261 ttttggttac cgtctttgcc ctgtccttgt tatccttgaa catattattt tcgaggagag
    34321 cttcggcata ctggtaggag gccttgatag acttctcata atctccgagt atccacggat
    34381 caagcttgtt tgaaatcgga tacaggactg aagcagtgat tttggtgtcc ttgctttggg
    34441 caagcttttc ctccatgaaa tctatgcata gccttatgga ctggacagga acccacaagt
    34501 ctgtaaacag cgggtgcctc acaagggggt ctatgggccc cagttccgaa ataggcccca
    34561 tcacaatctc atctgcaccg atagccacca tggtagcggc actttttgcc acaaaaggta
    34621 tgataacgct gaagttatca caaaattccc tgatcaggtt gacgatttta tagggagtat
    34681 ccacagcacc tccataactg tttagaaaaa ggtctatcct ttctgtcttt cctatgctgc
    34741 aaagctgttt gtacagagga accagtacat aatcatccat aggggagaag gatatatatg
    34801 ataataatct ggaattccgc atcttttcaa tgcctttgag caggttcagc ctcttcttgt
    34861 tcgttatttt caacacgcaa atcccctctc gctcacgaaa aactgaagac agttttttct
    34921 actacacata atatgtgctt tccgatgttt tgtgatgaaa tccatgtcag gcatgcaaaa
    34981 agtataaata gaaccaatat tgaaataata tgatgtaatc gcatttttct gtaaagcatt
    35041 caggaggtga agcttgtgcc gccgctaaac gatggtgaac tcacaagtat gaagctctca
    35101 attataaata atttggaaaa gacactgaaa atcgtatatg aacatcagta taggaatata
    35161 ttgtccaaaa ttgtgagcaa taagaaaaga ttcataagaa aacgcctgcc tttggatgcc
    35221 tcagaggata tgtgcagata taactcactg gctgcagggc ttaccaatta cctgataaac
    35281 ctttttgacg tattcggtga aagtgcggtg ctgaacgcaa aaaaaacctt tgaggaaaat
    35341 gggtcaaaat gggggaagaa gctgcggaaa aagcttttga tgcaatcgga taccaatgat
    35401 attaaccata ttgtcaagta tctctatata gatgtccctg aaatagatta tatggaaatg
    35461 tccgataacg gcttggtatg gcatttcaac aaactgggcc acagcagcat caacgaaggc
    35521 ttcgcaaagt ttcaccctgc cttctataat ataaaatcag cctggctgca ttcctttata
    35581 aagtccttca ccgcccagta caccggtatt tttgaaaaga aaagcgaaga tgacaatgaa
    35641 attgtcaccc gcattgagtt taaagaggat cagtagaaaa ttgcacagca attttctact
    35701 ggctgcgagg tgcggggttg cgaggtttcg aggaaaagct gggtattcct tacaagtaaa
    35761 tttacgtata acactaagaa cactaaaatc cacagaagac acggagggaa aaatattccg
    35821 atatattgat aatgatcgat aataattaat tgacgccgtg ttattccgaa agaatccgtt
    35881 aagcagacct gtgttcagtg ttattcgtca ttatcaattc tcaccctatt gaagcattaa
    35941 gttactttct aaatacaaac aaatactcgt gcgctagtag cagaaaatta tacttcacac
    36001 tgttggtttt ccaatatccg gttgctttgc agttgtgttg ctccttaatt attatttctt
    36061 tggtcttgaa ccctgcttcc tcgaataaat tcattacttt aaagcccaaa ggaataacat
    36121 gccctttttg acgcatatca cccataagta tagcgcaaaa ccgattcttc tttagcacac
    36181 gatagctctc gacagccacc tgtttcattt ctacaagaaa ttccgggact ttacattgcg
    36241 ataaatcctc agaaatatca tcactgtatt gaataatgtt cgcatatggg ggatgtgtgc
    36301 agataagatc aatgctttca ttgattatac tatcaagttt ccgcgcatct ccttttgcaa
    36361 ttgtaacttg tccactgttt tcacgctcaa agttacactt ttcatgacag cgttcaatcg
    36421 caacatcatt aatatccata ccaattatat ttctgttaag tagtttcgct tctacaagcg
    36481 tagttccgcc accagcaaat tggtcaagta taagatctcc ctcctgcgaa tatcttaaaa
    36541 taatattccg tggtatataa ggagaccaat ttcctctata ttttgcgtca tgcgtagccc
    36601 aatcgccgcg tattggaaaa ctccaaacgg tattcatctc taattcaaaa ttttctggtt
    36661 cccatttatt tatcttattc tccattcttc ctccttaaat tagcttaaaa tatctcatgc
    36721 tatcttagct tccaattatt caaataaatc atccataact ccgttttcca tatcatcaat
    36781 gctatataaa tgtttcataa catcaaaggt ctcttcgaga ttgcgtcgag cactgttcca
    36841 acccttacca tctgtaaacc atacaaatgt aaagccatct atggtatctg cctctaatgc
    36901 aagggtttta taacttcttg ctgtctcatt caacttggag ccactactac tataaaaatt
    36961 agtctcaata ccataaatca tgtttcctgt tttcactaca aaatcaaagc gcttttcagc
    37021 tttgccttga ttggatatag cagataagtt aatactccat tttctctcta tatcactgag
    37081 gttcatttcc ttgaaatagt ctattccatc tatgaatccg cttttcacaa tgtgactctc
    37141 tacgagttct tccataagat gtccaccacg attttttctc ccatttgagt caagccccgt
    37201 ttcaataccc gttacatagt ctaagagatt gcttacaata tgattcttta tcaaatcaaa
    37261 taatcctgtc ttatgcataa aaaccttata ctgttctatc gagtagttca ttctaagaaa
    37321 agaatagttg tatgaccctt cagtatccat tgctcttatt tcgttggctc taacagcaag
    37381 taatattgga atacatttaa gtgtttcggg gtatttataa acaagagcct caaaatcgtc
    37441 ttctatatta tctgaaccaa ttaatgaatt tagaatattc aattctactt taattccgtt
    37501 cacattccta tggacttttc caaaatcaac ataataccca taatcagaaa tactgtctcg
    37561 aaactttgaa aaccataaat taaaatctct tgccatataa atcctccatc atatttatga
    37621 cataaggcct ccgccgccct gattctttct tgcgtaagca gttatccatc cggtttgacc
    37681 tttaatgctt atggatttgg gaggttcaag ccccatatta tcagcactta tacaaatctc
    37741 tctaaacgga caagtatcat aattggggtc tgcaatattc gaacgcacaa aatcagtctc
    37801 tcttatcgca atactaccat cattatctgt acacggaagt atcttcttta taagtgtagc
    37861 tttcttatta cggataccat ttgtgtagca aatctgacat gttctgtttc tagaagaatg
    37921 ccacttaaat gtatgatttt ccacatcgca ttggaattca cataaaattt ctttacagaa
    37981 ttgtttacct attacattat atacaaaata atctagcaag catggactac ctataacttc
    38041 attgggatat tcattacacc atatctccca tacctttctc cacgcaagcg catttagctc
    38101 atctacgtat ccatattgat agcaaaaaat atcaatgaaa ctcgaaacta aaggaatcgc
    38161 agcttttatg atgccggttc ttaatgctac ctttattgtg ttaatatcgc ttgcaacatc
    38221 gattttctcg aaacctgtca cattatccca gactcccaat actaccatat cacgtataag
    38281 catatctgct ttcttatttc catatccggc atttggttcg caaataatag catttcttag
    38341 ttcaaaaaca tctctatcgt aataatcaat aacattgtaa ggctgaacac tacgatccag
    38401 taggaatttg ctaatatttt ttgcaaattg tactcgctta tcactctgag ataggcccgt
    38461 gataccaatt gatctcacaa aatctgccgg gtcgttgaaa attgcttgtt catcaaagat
    38521 actattatca gaaaggaaaa cactagccaa ttctctaaaa ctacgagaac cacgaaaatc
    38581 ttgattagct gtaagaaaac gtattctaca tgtatcttca tttgataggc cattagtatc
    38641 atagatctcg aaaaacactc tcccacctgt cctaatatat acctcgtgaa gcaccgatgc
    38701 aacatcatgg caaacatttt catctatttc aatattcaat atctctctat gatgctcata
    38761 ccaatactta tgaaaatccg atatgaattc aaattcttct cctagtgctt cgactgaacc
    38821 tattccttca taattggcat ataaaaaatc atttatttgc ttgacaacat tgcgtgcttg
    38881 ctcaattgta agcgattcag gattatcagg acatatttcc gcaatccccc tcgcaaatac
    38941 atctaattct gacatattgc ctccttaaaa attacagata agtaattcgc taattttgcc
    39001 tcgagactca gcattactgt taatcatcct tgatgcctca actcgcttaa tccgatgaga
    39061 tgaatataat tcatcaaaga attcatcttc cgaattttcg ttttttgggt ctgaattgct
    39121 aacaataacc tttgctccac gcttagttaa aagattgaca tattcagcaa gttcgcgttg
    39181 tgccttgtca tcaaaaccat cttcagtata tgaagtaaag ctagaagtag tgttaagagg
    39241 tcgatatggg ggatcaaaat atacgaaagt gcttttgtcg ataaaaccag atgacttcct
    39301 ataatcacca catataattt ctacgttttg aagtgaataa gaaatattca acaaattttc
    39361 agcatcgcat atagttggct tcttataggc tcccataggt acattaaaga gtcctttgcg
    39421 attgactcga aatagtccat taaagcaagt tctgttaaga aatataaaaa cagctgcact
    39481 ttcaagattt attgaactga caccgttgat ttttaactcg ttgaaacgtt tgcgtttggc
    39541 ataataatat tctttgcgct gagcatcttc caatggaata aactcatttt gatactctga
    39601 caacagtccg ataagttcat ctccttcatc ccgaatcata cggtaggcgt taatgagttc
    39661 agcattaata tcactaatat acatagcatc aagattatac gaagataaaa tatcaaataa
    39721 gactgcgcct ccgcccacaa agggttcagc atattttttt atgtctacac ccaatcctac
    39781 aggatatgtt ttgcgtatat ccgtaagaag ttgccccttt cctccagccc atttaacaaa
    39841 gggttttagt gatatagttt gatatttttt atgcttaagg cttcgccaat caattggttt
    39901 cttcgcatct gtaggtataa tacacatatt actgcacctc tttatactta acttccatat
    39961 aaaatattat attctacaat tgtgataata gcaaaaaaaa catatgtagc ttttgatatt
    40021 aaaaacagca gtagaaaatt acacactagt gtgatctagg ttaatagctt gcaaggttag
    40081 gaggtttcgg gaaaagtaaa tgtcatccta aacgcagtga agaatctaag attcgaccca
    40141 agttcagaat gacaataaac ttttctcgtg tctcgtatct cgtgtctaaa aatgtgacct
    40201 tgtcacattt tttacaattc gtttactatg ctgacagaag cgctggctcc tattctggat
    40261 gctcccgctt caatcatctt tagtgcactt tccagatttc ttattcctcc cgaggccttt
    40321 acacccatat cgggccctat ggtccttctc atcagtttta tatcctctgc tgtcgcaccg
    40381 ccaacggaaa agcctgtgga agtctttaca aaatcagcac cggcttccat ggaaagcaag
    40441 catgccgtta ccttctcttc atcagtaaga aggcaggttt ctattattac cttcagcagc
    40501 gccttatcct tgcatacacc ggctacttcc ctgatatcct ctttcactgc atcaagcttg
    40561 cctgatttca gtgctccgat attaattacc atatctattt cagccgctcc gttttcaata
    40621 gcctgtgacg cctcaaaagc cttagcctcc tttgttgttg cccccaaagg aaagcctgca
    40681 actgtgcaga cccttacctc cgttccctga aggaatgaat aggccagctt cacataagaa
    40741 gcattcacac atactgacgc aaacttgtat tccaaggctt ccttgcaaag cttaattatg
    40801 tcaccctctt ttgcatcagg cttcaacaat gtatgatcta tataacgggc caactcaaac
    40861 ttattcattt attctccccc attatgtgca ttgcttatcg aattatttct cccaatgcac
    40921 cattctgtat accggctgca tttgcttctt ctatatcaat atgcatatcc atagcatatg
    40981 taggatgaac tcttaccatc acgttttcca atactaatgc cctttcatcc cctattctta
    41041 cttttacaac gtctctgtcc ttaaggccga acctctcgct gtcgcttgta tgcaaatgta
    41101 tatgccttgc cgctactatg actccctctt ttaattcaac tttgcccttg ggccctacaa
    41161 tggtgcagcc cggggtaccc tgaattttcc cggaaggcct taccatgcct tctactccca
    41221 tggtccttgc atcaaacatg gaaatctcca cttgggtatc cggtctgacc ggccccagta
    41281 ttcttactcc cttaagagtt gatttgggtc ctactatatc tactttttcg tcacatgcat
    41341 actgcccggg ctgcgaaagg tccttgatgt tggtaagctc atggccttct ccgaacagta
    41401 tctcaagatg ctccttagtg acatggatat gtctgtttga catgttgacc ggtataaatt
    41461 tctccataat aattcctccc atcgaaattt aatttttcaa tttttcatat gccttaatca
    41521 ttatagcaca ctctattctt ttcttctgca gtttgcattc aatgtcatag ggttccttaa
    41581 ggttattgtt gaaagcagcc gcatttgcct ggcatccccc gctgcagtaa aaccttgccc
    41641 agcactccct gcatttctcc ttatggtaaa cggtgttccc ggaaaactcc ttctgaagtt
    41701 ccttatttga gacacccttc catacgtctc ccatcaggta ttcctttctg ccgacaaact
    41761 gatggcaggg atataattct ccgtccgggg taactgcaaa gtattccacc ccggaaccgc
    41821 aggaagacac cctcttgtaa acgcaagggc ccccgtccag atccatcagg aaatgataat
    41881 acctgaagcc ttttcctttt tcataataat ctatgtattt ttctgcaagc ctgtcatatt
    41941 cccggtaaat ccttggaagg tgctcctcct tgagagcata agcctttcct ttttctgcgg
    42001 caacaggttc aatggagatt tccttaaaac cctcgtcggc caaatgcagt acatcactgc
    42061 agaaatccag gttgtttgcg gtaaaggttc ccctgatata ataactttta tcccctctgg
    42121 aggcaacaaa gcttttcagc ttcggcatta tatcatcgta agtgcctgcg ccgcaggtaa
    42181 actttctcat ccggtcgttt acctgtttcc ttccgtctat gctcaatact atgttgtcca
    42241 tattctcatt tatgaattca gctgtttcct tgtccagcag tgttccgttg gtagtaatgg
    42301 taaagccaat cttcttgccg tactcctttt ccctttccct ggcaaaaaga acagtctcct
    42361 ttaccacatc aaaattcatt aaaggctctc cgccgaagaa atccacttcc agcctttttc
    42421 tgttccctga actttcaaga aggaattcaa cggcccttga agccactgaa aaaggcatca
    42481 gcctgcgtcc ctcatgataa tctccggtac ttgcaaagca gtagccgcac ctcatgttgc
    42541 agtcatgagc aacattcagg cacaaggcct tggtatacat ttcctttttg tcctcaagaa
    42601 ccttgtccat gtatacatca gcggaattca atagtccttg gctttcaagc tcccttacct
    42661 cgttccatgc ttcccttata ttgcattcct catgtttgcc cttaagtact ccgacaatat
    42721 tttcaacact gtccggatag tggtccaata tatcccaagt cagctgatcg acgacatgca
    42781 ccgctccgct gttgacgtcc acaactattt tcaagccttt ctgttcatat ctgtgaagca
    42841 taagtgaccc ccg
//
LOCUS       DAOOAB010000020        39623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1310_length_39623_cov_16.030732, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000020 DAOOAB010000000
VERSION     DAOOAB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 39623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39623
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_39623_cov_16.030732"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..488)
                     /locus_tag="PKU88_06165"
     CDS             complement(75..488)
                     /locus_tag="PKU88_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPX68906.1"
                     /translation="MNIAFFLTPKEEVIWISADSTMRQAIEKMEFHRYSALPLVDSGG
                     KYAGTITEGDILWHLKHSKNLEFKDTNKIMIRDISKYQNNKPVSIESHIEDLILLSIN
                     QNFIPVVDDHDIFIGIIKRSVIINYFYKLLLNRKI"
     gene            735..2258
                     /locus_tag="PKU88_06170"
     CDS             735..2258
                     /locus_tag="PKU88_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPX68907.1"
                     /translation="MMRVNINRNSDTPVYLQIKNQIRDMIISGELPAGHILPPERILS
                     ELLNVNRTTVAKAYHELKADGLVQARVGKGTMVAVKHFKGRFNNIRQFNPIPWYQFYN
                     ESVDSGGDHIISDIMDINCTEDIISFSGGFPSPELYPLEELSSIQEYLFKTVGKQLLL
                     HSPVEGLTSLRAGISSLMKSRNINISPKDIMIVSGSQQGLDYIARVFIKPGDTVILEE
                     PSFFGAMQIFRSAGARIIGAPVDSCGMKTDMLESLIQRYKPKFIYALPTFQNPSGIVM
                     SLERRYELLDLSYKYRVPIVEDDPYGDLRYEGKAIPSLKALDPYEHVIYLSTFSKVLS
                     LGLRIGWVAAPQQVIRKFAYFKQITDMHVNTPGQYLISEFLSQGCYEKHLNAVLTEYR
                     KKRDLMNAALEKYKLPEVLWEKPEGGYYIWCRLPEYVTNNRLVSKAAEKGVVYLPGEA
                     FYPDGTQGENYMRLNFTFPNSNKIEEGIKLLMDALTEVEVRDGSNSAGRSYSRSPIV"
     gene            2289..3182
                     /locus_tag="PKU88_06175"
     CDS             2289..3182
                     /locus_tag="PKU88_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPX68908.1"
                     /translation="MEIKIKQVDAFTGIPFGGNPAGVVTDAAGLSDELKQKIAREMNL
                     SETAFVSPSKAADFKVQFFTPRFEVDLCGHATIGTFSALHEEGKLPSDKNIFYQETKA
                     GILPVELTSINNEKVFMMTQALPKFENIDLSKSEIAGLLGLSKDDLMDTPDLMVNTGI
                     WWLVFGVKTLDKLMNAKPDLKTIERISEQYNIVGITPFCLEALDPKYDYHLRAIAPFV
                     GVAEDPVCGTGNGCVSSYIVQNGLVKCDDTVNMIGEEGQEVNRPGCVYASISKKEGEI
                     SRVQIGGTAVTILQGVIKITD"
     gene            3201..3890
                     /locus_tag="PKU88_06180"
     CDS             3201..3890
                     /locus_tag="PKU88_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HPX68909.1"
                     /translation="MKTIGIIGGVSWESTVEYYRILNETVNKKLGGLHSAKCLIYSVE
                     FQEITDLMQTGDWKAISVRMQEIASILKSGGADFLIICSNTLAAPAPEIEKNIGMPVH
                     SIAEATAKEILGMKLKKIGLLGTKYTMEGDYYRNTLYKYGIETVVPEKEDREAINSII
                     FDELCLGILKESSKEIFRNIISKLQSKGAEGIVLGCTEIPLLVKQEDVSIPVFDTLTI
                     HAVSAAELALG"
     gene            complement(3977..4810)
                     /locus_tag="PKU88_06185"
     CDS             complement(3977..4810)
                     /locus_tag="PKU88_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPX68910.1"
                     /translation="MNKPKVALIQMHVEEDKLRNMVKASDFIRKAAEQNIDIAVLPEM
                     FNCPYKTSNFPVYAEEEGGECYGMLSELARKYQIYLIAGTMPEKDDKGRVFNTCYVFD
                     RKGNKIGKHRKMHLFDIAVEGGQHFKESETLTAGNTVTVFETEFGKMGVAVCYDFRFP
                     ELSRLMVEEGAKVLIAPAAFNMTTGPAHWEVMFRSRAIDNQVYTIGTAPARDVDSCYT
                     SYGNSIIVSPWGEVVARLDEKEGLLIETLNLDYVEKVRRELPLLAHRRKDIYKCGKKE
                     E"
     gene            complement(4879..6873)
                     /locus_tag="PKU88_06190"
     CDS             complement(4879..6873)
                     /locus_tag="PKU88_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPX68911.1"
                     /translation="MIKNFKLSLQIGAVFIGTVVGAGFATGQEIMQFFTCFGQIGAVT
                     LLLSGILFYLMAAAVIRAAAYYNAYNYKELIYQVAGNRIGFVYDILVTAFLFIGTSIM
                     FAGSGALFHESLGLPHTLGVVLMAVLTFIIILHSLTGILNINSVIVPILFAVITAVLA
                     ATIMNGDIGGFGLRLSENYDGNFFKPVFYFLFFCSYNTFLSIGVLSAIPEKVKNLPVL
                     KTGVFLGAMGLMLLSLMLNISLTLKSPQVFNYSIPMDCITSGLGRIIKSVVALCIWCE
                     IFSTAVSNAFSIAKRLSKNRHITYKQTCFFTVLCCLPLAFLEFKGLISFFYPLFGALS
                     MFIIFRLLFASYGFGGKTKKVISLVSILCMITVFAYTANNPQPCFTYIEPVESSYDIT
                     MKQDLLCLMMAYPEHIASIEKLEDRVYVTMKSGRKILYDDKKIKDMEAKIAYPDLQDM
                     MEPVYPIGLVKKLMDINYDPGRARVYSLLNEVYGGSRQQIEGNLVNVNTGCGAFQFNR
                     NNKAGEAFKNVMDELASAAKNRKEIRACVYPCSGTYNYRVISGTNRLSPHSYGIAIDL
                     ARDKRDYWQWASREEGQKRLSSYPNEIVELFERNNFIWGGKWGHFDILHFEYRPEILI
                     KARYFGNSRDIHKPWYNEVLLEDAYAAEYIRKIDKALDGN"
     gene            complement(6942..7859)
                     /locus_tag="PKU88_06195"
     CDS             complement(6942..7859)
                     /locus_tag="PKU88_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HPX68912.1"
                     /translation="MKKITNISIIGLGAIGGSYAERLYQNNNVNLTVVADRSRIEKYN
                     TNKLTVNGKPCDLKFIQPDSDEAPADLVLIAVKYNNLSQAVDAIRKRVGPDTVIMSLM
                     NGIDSEEIIGEAFGMEKLLYSSCVGIDALREGNNIRYTSFGKLHFGERENKLHTERVE
                     RVKVLLDESGIPYHIPENMIKALWWKYMVNVGLNQVSAVLGAPFRVFNTVQEIRELVD
                     AAMKEVIDVSIKAGINLEEESMKEVHKLVDTLSAEGKTSMLQDIEAGRKTEVEMFSGV
                     LRKIGRKYGVPTPVNDTLYRMIRTLEQME"
     gene            complement(7992..8495)
                     /locus_tag="PKU88_06200"
     CDS             complement(7992..8495)
                     /locus_tag="PKU88_06200"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX68913.1"
                     /translation="MKAGSIQLRQEVYQADAWKIADWLDDYEVIKYLNEGRNVVKSIT
                     EAIQRVNMPILTHLFNQKGSFFMIMEKKQPIGFLRLVPKGKTAEMVIVIGEKEKWGRG
                     IGKHAIMEGLHHAFFEWRMDEVIAKINLKNERSIRAFKSVGFKPEKELMKEMQYSISI
                     REFLKIG"
     gene            complement(8780..9673)
                     /locus_tag="PKU88_06205"
     CDS             complement(8780..9673)
                     /locus_tag="PKU88_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX68914.1"
                     /translation="MAVNFKNLPDEELLYGAKTCQGCGVTLAARLALKVLGEKTCLAT
                     PACCFAATTTVYPQSSIFVNNAITAFPALASTLSGMAVAAEALGWDEDITILGIAGDG
                     GTVDIGLQALSGAAERNENIIYICVDNEAYMNTGNQRSGVTSYKAWTTTTPTGSCSKG
                     EKNKFKKSLFEIMAAHRVPYTATASVAYPKDFMEKVEKAKNIKGCKVIHVMTPCPTGW
                     GYDPAITVKTAKLAVECGLWYLAEFENNEFRFNFVPKEFKPVEEYLKLQRRFRHLDES
                     DYREIEANRDIEWDRMRKRFA"
     gene            complement(9673..10845)
                     /locus_tag="PKU88_06210"
     CDS             complement(9673..10845)
                     /locus_tag="PKU88_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPX68915.1"
                     /translation="MSKKAFLSANEAAAYGVKLSRPHVVSAYPITPQTTLVEKIADFL
                     ANGEADFKYMMVESEHSAMASALGASMMGARTFTASSSQGLLYMCEMLSYVSGSRFPI
                     VMVDANRSVATPWNIFGDQRDAMAMRDSGWIMLYVESGQEALDTVIQAYKIAEDPEVM
                     TPVMINIDGFTLTHTYDMVEIPEQEEADSFLPPYSTTNKMDMKDPKTLCMSVGPDFHT
                     EGRFLQQKAFEAAKRKIVSVNDEYAKLTGRSYGGMIEEYRCDDAEAVLVAVGSVAGTV
                     RVAVDEMREAGKKVGLIKLRSFRPFPKEYFESIRDKFKAVGVIDRSISFGFDGTVFSE
                     VKSAMYGSGAKMANYIVGLGGRDIPKHQIETMFSKLLGMASGKNEAEVQFLGMRWE"
     gene            complement(10842..11150)
                     /locus_tag="PKU88_06215"
     CDS             complement(10842..11150)
                     /locus_tag="PKU88_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX68916.1"
                     /translation="MNKPKWNKDYKAPSSWDEMFFGSSNGVGILPTPNSGFRTLHPEI
                     DSKACIKCMMCWVFCPEGCIDKSGDHLEIDMDYCKGCGICAAECPKNCIKMIKDGDTE
                     "
     gene            complement(11150..11716)
                     /locus_tag="PKU88_06220"
     CDS             complement(11150..11716)
                     /locus_tag="PKU88_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPX68917.1"
                     /translation="MKQIRWHGRGGNGAFTAARLLGVAASVFEGKSAQAFPSFGPERR
                     GAPVLGFTRIADKTITDHSQVYECDCVIVLDETLCDVTNVAAGLKPDGVLVINTRKTA
                     DEAARHIGFEDPSRIVVLDATDIALELLGNPIVNTIMLGAAVGATEMVSMESVEKAID
                     DVMGRNLREKNKKAARAAYDKVKEAVMQ"
     gene            complement(11713..13167)
                     /locus_tag="PKU88_06225"
     CDS             complement(11713..13167)
                     /locus_tag="PKU88_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPX68918.1"
                     /translation="MALKTGAQYIEEMKKMRPNVYKWGELIEDVTEHPATASHIRNVA
                     KWYDNSFDPEKEQLYTNTSFLSGNKAHRWNTLITSADDMLGNANMKRDGYHTCGSCMG
                     PVCAGWTVLNALWSVTFDMDQEFGTDYHERLKNFCIFAENNALALSGALTDAKGNRAL
                     KPSQQDNPDMYLHVVEKREDGVVIRGFKNQICGTVGSQYIMVMPTTGMGEDEGDYAIA
                     AAVPRDAEGVTIVETRRPSDTRIEEEGWDGIKSGTTQAYIIFDNVFVPSKHVFMNGET
                     KYTGKLIGNFTAIYRAAIGACVAGQGDVMIGAALGMARANGLAQKAFQDKLTKMAINN
                     ETTYGLGIGAMYTGKKHKSGAFYPNPLLAHVNKVHVATLPYDTKVLAQEISGGITETG
                     CMPSYKDMMSPLYGEKLIESLRSAVPGEDRIKMARLVEWLTIGGGVPGCMHGGGSPDT
                     AKMVVKAATKWDNYVDYARALAQVESPLKEEKKK"
     gene            complement(13181..14167)
                     /locus_tag="PKU88_06230"
     CDS             complement(13181..14167)
                     /locus_tag="PKU88_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPX68919.1"
                     /translation="MKKIWVFAEYVNCTLDRVTLELLTKAKELASGLKGETEVAAVLI
                     GSRIGSTCKTLAEYGAEKIYLVESDELSLYNPMLYAPIITELVQKEDPDIFLFGATAI
                     GSELGPTAAAQLKTGVAAHCVDMHIDENEHLVAAVPSFGGKILGEILCPDTRPQMASV
                     KPGIFVQGEPEEREAQIIKVDVSAITADNLPLKAISLEKKETEGVPLDKAEVVFCGGY
                     GIGCKENWGKLEKLAAIFGGAAACTRPVVDEGWAPGEHVMVGTSGKSIRPKAYVGFGI
                     SGATHHICGMKDSGLIISINKDEKAAVFEVSDAGAAADLNRILDQLMIKLDL"
     gene            complement(14164..15012)
                     /locus_tag="PKU88_06235"
     CDS             complement(14164..15012)
                     /locus_tag="PKU88_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPX68920.1"
                     /translation="MALKIVVCIKPVPDPDYYDKVSIDPVTKRITREGIPTIINPVDK
                     NGIEAALKLKAQYGGKVTVLTMAPPNATENLKEALAMGADEAVLLSDRAFGGADTLAT
                     SYTLAKGIEKLEGFDIVFCGTESADGATAQVSSQLGEWLGVPHLWNVFSLENLEERTL
                     RVRTKAENGFMEWEVKLPCLLGVSRELNKPRFISAMGIIKAKNKPITTWGKADLDAEE
                     ERLGLAGSPTKAGAIFTPDLKRKGEMLTGSAEEIADAIIEKLRTAGVNIPTKGDACII
                     EGGARQ"
     gene            complement(15053..16207)
                     /locus_tag="PKU88_06240"
     CDS             complement(15053..16207)
                     /locus_tag="PKU88_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPX68921.1"
                     /translation="MQFFEFTEDQELLRKAVREFAEAEVAPKAAEWDENDYCPMELFP
                     IMGELGINGIFAPEEYGGAGMGHVERAICIEEISRYSAGLGIALMTHHLGMSPILDFG
                     TEEQKKKYLPELCAGTKLCGLAVTEPGGGSDFAGQKTTAELVDGQWVLNGRKCFITNH
                     HADVNVITAVTGQDEKGRNQLSAFIIEKGTPGFGAGRKEHKMGLRGSVTGDVILNEVK
                     IPQENIIGNVGAGAKIGMAAIGEVGRAGMSAICVGILRGCVEEAVKFANERVVYGKPI
                     AKLQAIQFEIANIRTEYEAAKMLLYYAAGKKDAKLPATPDFAIAKYYSTEAAVRASKR
                     TMDLMGGYGVINEYPVGRFLRDALASIPSGGTSHIQQLIIAGNTLSAFKA"
     gene            16480..16893
                     /locus_tag="PKU88_06245"
     CDS             16480..16893
                     /locus_tag="PKU88_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPX68922.1"
                     /translation="MMLDECINFLLTKTQQVVFQQFKAGLAKYDVTPAQYGVLKCLWS
                     QDCQNPSQIACSLFLDASTITGILDRMENKDMIKRIADPKDKRSLKVVLTERGRLLEK
                     PVTEIIDEENKKVLHALNEGERKKLTEYLNRISSI"
     gene            16917..17786
                     /locus_tag="PKU88_06250"
     CDS             16917..17786
                     /locus_tag="PKU88_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX68923.1"
                     /translation="MIYDEPLFRPPSEAYSLIFQATIGCSHNKCSFCGMYKSKKFRIR
                     SKSDVFRDLEEMAARYNKARRIFLADGDALVLKTRDLKDILQKIRELFPCCERVGIYA
                     TPGDILRKSPEDLTSLKEHGLGIVYLGVESGSDKILKAVDKGADSKEIIEVGKRIVSS
                     GIKLSVTLISGLGGKDGWEEHARESARVINEINPDYLGLLTLLIEPGTRMHDLVQQGT
                     FKLLSAEEVALETQLLIRNLELRNCIFRSNHASNYFSLEGTLPHDKSKLLKQLDNILN
                     NIEDYSGEYFRRL"
     gene            complement(17806..18480)
                     /gene="plsY"
                     /locus_tag="PKU88_06255"
     CDS             complement(17806..18480)
                     /gene="plsY"
                     /locus_tag="PKU88_06255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPX68924.1"
                     /translation="MSTLYNSMINIAVIIILSYLAGSIPTSIIVCKAVKGIDIRKHGS
                     GNAGATNVFRVMGWKTGIFVVFIDIFKGFAATEWISRLNLSGGAPQSSMLVPILAGAA
                     AVIGHCFTIFAGFKGGKGIATSTGMLIALFPVPLLVSAASFAVVLLTSGYVSLSSLTA
                     AIVFPVTMTALYINNTDNIDQTALIFSIIIAVFIFYTHRSNIKRLIAGNENRFEKLRI
                     LAKKNR"
     gene            complement(18505..19926)
                     /locus_tag="PKU88_06260"
     CDS             complement(18505..19926)
                     /locus_tag="PKU88_06260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX68925.1"
                     /translation="MKNNRVASLSLKAGKAAKDGFLGAESFYIFDDHTDGVCIINERG
                     YIEYFNGAYERILKISDRIETGMNIFLTKNDDVLLTAFREKRCICGHLVLYKGAAHVS
                     ASASPIYSNGEFKGVVAIYREEAAQDICRKKDTVIEFNSPPVNYMNNIRTMFKDIVGE
                     SSSIMKALKIAYKAAQTSSNVLICGESGTGKGLIARAIHNNSSRAKGPYSIVNCGAIP
                     STLLESELFGYERGAFTGAVRRKTGKFEQADGGTLFLDEIGDMPPDMQVKILRVIQDK
                     EFERIGGCDTIRCDVRIIAATHCNLEALVEKGAFREDLYYRLNVLPIYVPSLKERRED
                     IPMLLEYFIKRISINMNKEITGVSPEAENCLLNYDWPGNIRELENLVERVIALTDGNE
                     IDINSLPSKISSLYCVNRHTCSAPALINMNNSGNIATLEEYEKEIIKEALARFGSYNA
                     AGKALGITHKTVALKARRYNIVI"
     gene            complement(19957..20319)
                     /locus_tag="PKU88_06265"
     CDS             complement(19957..20319)
                     /locus_tag="PKU88_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPX68926.1"
                     /translation="MERNIVTCTICPIGCSLEVIGDSSSDSGYLVKGNRCNRGIGYAI
                     AEVTNPARILTTTVKLNNAILRRLPVRTDKPVPKNLLLDCMKVINSVEVDTPIALGAV
                     IIENILSTGANVISSRTV"
     gene            complement(20332..21582)
                     /locus_tag="PKU88_06270"
     CDS             complement(20332..21582)
                     /locus_tag="PKU88_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX68927.1"
                     /translation="MNEYDIAVIGGGPAGLAAAIEAKKHGGTVLLIDREKELGGILQQ
                     CVHNGFGLHIFREELTGTEYAERFIKEFKELGIEYKLDTMALNITEEKVISMVNKTEG
                     FVNIKARAAILAMGCRERTRGAINIPGTRPSGIFTAGTAQRFVNKEGYMVGRNVVILG
                     SGDIGMIMARRLTLEGARIKVVAEALPYPGGLIRNVVQCLEDYGIPLLLNHTIVDIKG
                     KDRIEGVTVAELDENRKPIKGTEQFYECDTLLLSVGLIPENELSKEADVELDPITGGP
                     VVNESMETTVKGVFACGNAVHVHDLVDYVTEESRIAGRNAARFVNGETGGYGPAIRTK
                     PGNGIRYIVPHFIRKDKVEQSVDLFMRVNKIFTNKKLVVKFGDTVIKIIKKSYMRPGE
                     MERICITAESLKTAEDLSLTVDVE"
     gene            complement(21595..23079)
                     /locus_tag="PKU88_06275"
     CDS             complement(21595..23079)
                     /locus_tag="PKU88_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPX68928.1"
                     /translation="MYDIAVIGAGITGCSIARELSRYNVRILLIEKNSDVADGTTKAN
                     SAIVHAGYDAKPGTLKARFNVPGALMYEKLCSELDVPYKKTGSLVLAFSPEEMKTIQK
                     LYDQGIANGVPDMEIIGKDKVQEIDGNLSDEVVGALYARNAGIVSPWELAIALAENAV
                     ENGVELFLDSEVTKIEKMNGLYRLHMGDRLAEARFVINCAGLYSDKVNEMVAAPSFKI
                     LPRRGQYNLFDKSLGNFVNTVIFQCPSDFGKGVLIAPTVHGNILIGPDAEDIEDKEAI
                     QTTPEGISYIWEASARSAKGLKPNAVITAFAGIRARSSTDDFVIGESKEAKGFINVAG
                     IESPGLTAAPAIAVHAADIVKGLMGGLEENLGFNPVRRKVVRFMELSQADKNLLIKQD
                     PRYGRIICRCESITEGEIVDAIHRKAGARSLDGVKKRVRPGSGRCQGSFCGPRVMEIL
                     ARELDIDIKDVVKNSPKSYILTGETKDNSLYEDVHDLAAASGKV"
     gene            complement(23067..23240)
                     /locus_tag="PKU88_06280"
     CDS             complement(23067..23240)
                     /locus_tag="PKU88_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68929.1"
                     /translation="MLHYYTQDICFQCKALERGVAVTTAIDSNAVVEQTDKYHAAIRC
                     CKVLVLEELKCMI"
     gene            complement(23408..24898)
                     /gene="glpK"
                     /locus_tag="PKU88_06285"
     CDS             complement(23408..24898)
                     /gene="glpK"
                     /locus_tag="PKU88_06285"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPX68930.1"
                     /translation="MSKYILALDQGTTSSRAIIFGNDGKIVSVAQKEFTQIYPKAGWV
                     EHDPMEIWGTQSGVAREAMDKIGIGADDIAAIGITNQRETTVVWERSTGKPIYNAIVW
                     QCRRTAPICDELKAKGLAETIRNKTGLVVDAYFSGTKVKWILDNVEGARERAKNGELC
                     FGNIDTWLIWNLTKGKVHVTDYSNASRTMLFNIHELAWDKEILEELDIPEAMLPEAKS
                     SSFVYGETDKEIFGAEIPIAGAAGDQQAALFGQACYKPGMAKNTYGTGCFMLMNTGEK
                     AVSSSNGLLTTIAWGLNGKVEYALEGSIFIAGASVQWLRDELKIIDNAAASEDMAAAV
                     ADTNGVYVVPAFVGLGAPYWDMYARGSILGLTRGANRNHIVRATLESIAYQTRDVLEA
                     MQEDSKINLQALKVDGGAVANNFLMQFQADILGVPIYRPEVTETTALGAAYLAGLAVG
                     FWDSKDEIAEKWNVDRVFEPVMDSSSRDKKYAGWKKAVSRTLKWEE"
     gene            complement(24956..25654)
                     /locus_tag="PKU88_06290"
     CDS             complement(24956..25654)
                     /locus_tag="PKU88_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HPX68931.1"
                     /translation="MSPFLSELIGAMILIIFGGGVCAAVTLNKSKSQSSGWIVIAVGW
                     GLAVGMAALAAPGTAFNPALTIANAVAGTLDWALVPGIVAGQMLGAIIGAAVVWLIYL
                     PHWAITEDKAAKLGVFCTAPAVRDYGKNFLTEMIVTAVLAFMCLAIGANKLADGLGPM
                     VTAAMITAIGCSLGGPTGYAINPCRDLGPRIAHAILPIAGKGGNDWSYSWVPVFGPIV
                     GAIIGTFLYMGLFR"
     gene            complement(25769..26335)
                     /locus_tag="PKU88_06295"
     CDS             complement(25769..26335)
                     /locus_tag="PKU88_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HPX68932.1"
                     /translation="MSNKFFPLIYKNPIIAAINDLNNIELAAESPCNIVFLLKGDISN
                     IAGIVDRFKSAGKGVFVHMDLIEGFSKDAVSLRYFHDNIKPDGIITTKSNLVRIAKNN
                     GIFIIQRLFALDSLSLASGIESLHTTKPDAIEILPGIMPKVINEVHGQTSIPVIAGGL
                     IRDKEDVIDCLNAGALGVSTSKKELWYM"
     gene            complement(26873..27820)
                     /gene="msrB"
                     /locus_tag="PKU88_06300"
     CDS             complement(26873..27820)
                     /gene="msrB"
                     /locus_tag="PKU88_06300"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPX68933.1"
                     /translation="MNKEIWLAGGCFWGVEEYFSRIDGVVDTSVGYANGRTENPSYHD
                     IPHTGHAETVHVIYDSEKISLEVILEHFFRIIDPTILNRQGPDVGTQYRTGIYYSDEK
                     DRETIEQAIAKEQQKYKVPIVTEVKKLEHYYPAEEYHQDYLKKNPGGYCHIDFSSLKA
                     VSRPKVDPALYEKPSRDEIKKSLSDDQYSVTQENATELPFRNEYWDNHKAGIYVDIVT
                     GEPLFLSSDKYDSGCGWPSFTRPIDQAVLKEREDNSHFMHRTEVRSRVGDSHLGHVFD
                     DGPKGKGGLRYCINSASLRFIPVEKMEEEGYGDYIKYVK"
     gene            complement(28209..28376)
                     /locus_tag="PKU88_06305"
     CDS             complement(28209..28376)
                     /locus_tag="PKU88_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68934.1"
                     /translation="MEDKVFKLLTKMYSEMTERFKEVRIKLDEKADKSDIVRIENELN
                     PKSIPCLMDVS"
     gene            complement(28475..29410)
                     /locus_tag="PKU88_06310"
     CDS             complement(28475..29410)
                     /locus_tag="PKU88_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPX68935.1"
                     /translation="MIKENLLSEIEAAGFRVKVFNAEHLKEISSDFKILSEQGALDKE
                     FYTKSLTVFNYDYKSVLSNARSVMVIASPQRKSIVEFQCGDKTVMAVIPPTYMYSGIN
                     NQIISILNNVLAVEGYSFGKLVIPLKLIAVRSGLGRYGRNNVCYIPGLGSYIRLSAYV
                     TDYEFEEDSWGDIKVMESCSNCTACIDNCPTGAIDKGRFLIHAQNCITNFNEYGTPIP
                     EWISPLWHNAIVGCMKCQEACPHNAKLIELVDERICFDKKETGMILDESAINELPEET
                     RRKISHLDMEDYYDVLPRNIRLLYCCSTQCGRQEG"
     gene            29519..30055
                     /locus_tag="PKU88_06315"
     CDS             29519..30055
                     /locus_tag="PKU88_06315"
                     /inference="COORDINATES: protein motif:HMM:NF023134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HPX68936.1"
                     /translation="MEFLCLEFVNSSWYITHKLFSDPLMNNEWLIKLFDKWNMQPLIE
                     PNELELAKLIDMRSRFMKLFTKIVEGKDLAKKDIELINSYMADISFFRQLQTKEGSLG
                     LYDIPKTRNWNWFMAEVSASFARLYSSEAINRLKICQNPECGWFFIDDSKSRNRKWCD
                     DTCASLMKVRRFRQRQKR"
     gene            complement(30196..30270)
                     /locus_tag="PKU88_06320"
                     /pseudo
     CDS             complement(30196..30270)
                     /locus_tag="PKU88_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(30428..31144)
                     /locus_tag="PKU88_06325"
     CDS             complement(30428..31144)
                     /locus_tag="PKU88_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68937.1"
                     /translation="MNNIYKVIKYQIHDFWKAVLIFYLIIILIASAITVTVSVNGENI
                     SFGGLEMATVIFLFVAGLNCFKSNFKFMLANNVSRKGFYIGNTIALVAVSAFMALVDA
                     VLNVILNNVLVSNIPYESVVMQLYGNNSFFAGFLWRFGLNTWAVCFGWFVTMLYYRCN
                     TIMKIVVSFLPVLAFIIYQHVDRILGGLADRAVIDFLTRAMGFAYNNNVYIGALSLIT
                     GSAVIALICLLPLRNAVAKG"
     gene            complement(31162..32022)
                     /locus_tag="PKU88_06330"
     CDS             complement(31162..32022)
                     /locus_tag="PKU88_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68938.1"
                     /translation="MKTIQIKGVSKVYGQTKALDKVDITIESNRIYGLLGRNGAGKTT
                     LLNLVTNRIFPTEGEILIDGETVFENDRVLGNIFYMTEQNLYPEDERVRNIYKWTKEF
                     YPSFDMEYAKDLSERFGLDTKKRVKNLSTGYASIFKAIAALASNADIVLYDEPVLGLD
                     ANHRDMFYKELISNYSENPKTIIISTHLIEEVAPVIEEAIIIKEGKLILKRSVEELLS
                     EAYIVSGEASKVDEYIQGRKCIGIDSIGSFKSATVLEAMNNKDEALAEKLELEFGKAE
                     LQKLFISLTN"
     gene            complement(31991..32386)
                     /locus_tag="PKU88_06335"
     CDS             complement(31991..32386)
                     /locus_tag="PKU88_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPX68939.1"
                     /translation="MLIDFNSEKPIYLQLAEAIEDNILKGIFEEETQIVSTTEISVKY
                     KINPATAGKGVNVLVDEGILYKKRGLGMFVSTGAKEKILEKRRNSFYKEYVQSLLEEA
                     AKLNISKEDIVKMIVGGNGIEDNSDKGSK"
     gene            complement(32536..32856)
                     /locus_tag="PKU88_06340"
     CDS             complement(32536..32856)
                     /locus_tag="PKU88_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative heavy metal-binding protein"
                     /protein_id="HPX68940.1"
                     /translation="MIITTTPNVEGRKVKEYKGIVFGEVISGVNFIKDFAAGLSNIFG
                     GRSGSYEGELIEAREAALREMEKRAGTIGANAIIGVDIDYEVLGQGGNMLMVTASGTA
                     VVIE"
     gene            complement(32937..33359)
                     /locus_tag="PKU88_06345"
     CDS             complement(32937..33359)
                     /locus_tag="PKU88_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPX68941.1"
                     /translation="MNIKVMYHTINGNTGKVAEAMAEALGVKAERIGEGSIAEPVDML
                     FIGDGVYGGRPDNSTVDFIKTLNGSMVKKAAVFGTYGMQEKAIKIMKELLEEQGINVS
                     AESFGCRGKCWAIMNRRHPSAEELLAAKEFAEVELNKL"
     gene            complement(33520..34626)
                     /locus_tag="PKU88_06350"
     CDS             complement(33520..34626)
                     /locus_tag="PKU88_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HPX68942.1"
                     /translation="MNYRELDTPALLIDKDIMISNCRRMQDYANRCNVALRPHTKTHK
                     MSRLALLQEELGAKGIAVAKVGEAEVMAAKGLRDIFIANEIVGEEKLRRIRKLAETID
                     ISFGLDSIPQADMIEKAFEGSKKQAVVLIEIEVGEQRSGVIEEAAFVELVNHLKKCSN
                     IYLKGIFSHDGHSYAAENAETCKEIHLKAQERTLRFAQLAAEAGFHLETVSIGSTPSL
                     MHDFPILDGVTEIRPGTYIFMDASQGNAYGSLERNAATVLTTVISLPTPERVITDVGA
                     KGLTAQTRNKGFTATKGLGLIKGWPGVSIHAVYDEHAIIYNKEFHDSIRVGDKVEIIP
                     NHICPVVNLHETAYIVSNGEVIEEIPVDCRGKLK"
     gene            complement(34651..>35669)
                     /locus_tag="PKU88_06355"
     CDS             complement(34651..>35669)
                     /locus_tag="PKU88_06355"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPX68943.1"
                     /translation="WKLDYKPPKFDMSLVDHDTWSIFRYRAFMPMEGAHWREISLGEG
                     ITPVIRFNEDVLLKMDYFMPTLSFKDRGAAVLIAHCKAIGVDSVVQDSSGNAGNSVAA
                     YCAKAGIGCEIFVPEGTSPRKINMIKSHNAKVNVVPGTRDNCADVCRAKVETEGKYYA
                     NHVYNPFFYEGTKTYIYEVYEQMERIPENLFVPLGNGTLFIGIVKALEELLESAVIDK
                     MPKIIAVQSEHCDPLAKAVITGEKHPVKVIPQPTMAEGIAVGVPMRGKEILEYTYKYK
                     IDIITAPENRIQEARAALAAKGIYCEHTTAATYAAYLKYCELNGRISDCLIPMCGAGL
                     KSDH"
     assembly_gap    35670..35769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36179..37582
                     /locus_tag="PKU88_06360"
     CDS             36179..37582
                     /locus_tag="PKU88_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPX68944.1"
                     /translation="MENRYVNFNEKRMYIFSELVKRYWNGGLKTAHDLTALADYIKRK
                     YGFSDYELPFIKDHIRVAMGLDPKGDGTFTDELDIIKSFREIDRPVVARIESPCEHCG
                     KLDCECGKCKHTEAGKYETHMYSRKSEPIISNDKCLNCGYSAPDCDFGALADKIEFIP
                     VIDLLKDEKTPVFACAAPAIVGQFGEDVTMGMLRAALKMIGFTDMIEVAMFADILTIK
                     EAFEFNQLVKSEEDFFLTSCCCPVWFNMIKTNYPDIYKHLSPSVSPMIASGRILKKLY
                     NDAKVVFIAPCIAKKAEIKEPDLKGAIDYVLNFRELKEIFNALNINPGELPADEKDQA
                     SFGGRVYARTGGVSFSVKTVVNRLEPTRVIKLKAKKVDGVKECRRVLDDLRDGRKINA
                     NFIEGMGCAGGCVGGPRTNIDVDKATKLVNEFGEDSFILTPFDNLNVMKILKQLGINS
                     IEEIMQNKEINKILSRE"
     gene            complement(37680..39509)
                     /gene="glmS"
                     /locus_tag="PKU88_06365"
     CDS             complement(37680..39509)
                     /gene="glmS"
                     /locus_tag="PKU88_06365"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPX68945.1"
                     /translation="MCGIVGYIGDKNAVPILMEGLRKLEYRGYDSAGVAVYTKENIEI
                     RKYKGRLSVLEEHLKKETLDGCLGIGHTRWATHGEPSDVNSHPHTNCSGDIAVVHNGI
                     IENYMSIKSMLIEKGYTFRSETDTEVLAHYVDYFYKDDLLEAVTKVLEKIRGSYAFAV
                     ISRREPDKIICVRKENPLIVGIGKGENFIASDIPAILNHTRRIYLLNENELAVIEKDR
                     ISFFDESGREVGKEVFEVNWDVAAAEKGGFEHFMIKEIHEQPKAVKDTMTSRVLPDSA
                     GIKLDDIKLTKEELQNFNKIFMIACGTAYHACMVGKYAIEKLARIPVEVEIASEFRYR
                     DPIVDEKTLTIIVSQSGETLDTLAALKEARRKGSRILSIVNVVGSSIARESHDVLYTW
                     AGPEIAVASTKAYTTQLIAIYIIALYMAKLKGTLKDEEIESIKDEMRRLPGLVEETLK
                     YKSAIQKFATHNSNARDIFYLGRGIDYAVALEGSLKLKEISYIHSEAYAAGELKHGTI
                     ALIEKGTIVLAVLTQDELYEKMVSNVKEVKARRAFVFALAKEGSTEVEKVADYTLYIP
                     KVSDILAPVVAVMPLQLLAYYMAVEKGCDVDKPRNLAKSVTVE"
BASE COUNT        10224 a         9814 c         7464 g        12021 t
ORIGIN      
        1 cgaaacgtcc ccgtgtcccc gcgtccccgt gtcgcattat tctattttta tgcgaaaaca
       61 cttctcttcc cgatctagat tttcctattc agaagcaatt tgtagaagta attgataatg
      121 acgctccttt tgattatccc gataaatata tcatggtcat ccacaacagg tatgaagttc
      181 tgattaatcg agaggagaat caaatcttct atatgtgatt cgatggaaac cggcttatta
      241 ttctggtatt ttgaaatatc ccttatcata atcttgttgg tgtctttaaa ctccagattc
      301 tttgaatgct tcaaatgcca cagtatatcc ccctctgtta ttgtacctgc atatttgccg
      361 ccgctgtcaa caagaggcaa agcgctgtat ctgtgaaatt ccatcttttc aatggcctgt
      421 ctcattgtag agtccgctga tatccagatg acttcttctt tcggtgttag aaaaaaggca
      481 atattcattg atatcctcct gacttccctt ggatattttt ttccggctaa ttatattata
      541 acgcatcgca ggaaattttt cgattgcaga aaaaattttt acctctgcgt gttacaacta
      601 tttggattat tccaatcaaa aattggatga ttaataaata tcagaattgg gggatttttt
      661 actgtaaaat atctgctata ataataattg gatgatgcca attcaataat acatttacaa
      721 gagagaagag tgatatgatg cgcgtcaata ttaaccggaa ctctgatacc cctgtttatc
      781 tgcaaataaa aaaccaaatc cgtgatatga taatctccgg tgaactgcct gccggccaca
      841 tacttccccc tgaaaggatt ttaagcgagc tgcttaatgt caacagaaca actgttgcaa
      901 aagcatatca cgagctgaag gccgacgggc tggtacaggc acgggtcggt aaaggtacca
      961 tggttgcagt caaacacttc aaagggcgtt tcaataacat ccggcagttc aaccccattc
     1021 cctggtatca attctacaat gagagcgtag attccggcgg cgaccatata ataagcgata
     1081 tcatggatat taactgcacc gaagatatca tttcattttc cggcggtttc ccttcgcctg
     1141 agctttatcc tttagaggaa ctgagcagca tccaggaata ccttttcaaa actgttggta
     1201 agcagctttt actccacagc ccggttgaag ggctgacctc cctgagggca ggcatcagca
     1261 gcctgatgaa atcaagaaat atcaatatca gcccaaagga tataatgata gtatccggct
     1321 cccagcaggg cctggattac attgcccgag tcttcattaa gccgggggat actgtaatac
     1381 ttgaggagcc ttctttcttc ggtgcaatgc agattttccg ctccgcggga gcaaggatta
     1441 tcggcgcacc tgtggacagc tgcggaatga aaacagatat gcttgaatcg cttatacaaa
     1501 ggtacaaacc gaagttcatt tatgcacttc ctacttttca gaatccttca ggaatagtaa
     1561 tgagccttga gcgcaggtat gaactgctgg acttgtccta taaataccga gtccccatag
     1621 tagaggatga cccctacggt gatctgcgtt atgaaggaaa agccatacca tccttgaagg
     1681 ctttggatcc ctatgaacat gtaatttatt taagcacatt ttccaaggtt ctttcccttg
     1741 ggctgcgcat cggctgggtt gctgcacctc agcaggtcat cagaaaattt gcatacttta
     1801 agcaaattac ggacatgcac gtaaatacac cgggccagta cttgataagc gaattccttt
     1861 cccaggggtg ttatgagaaa catcttaatg cagtgctgac tgaatacaga aaaaaaagag
     1921 atctgatgaa tgcagcactg gagaaataca aactgcccga agttctctgg gagaagccgg
     1981 aaggcgggta ttacatctgg tgccgcctgc cggagtatgt tacaaataac agactggttt
     2041 caaaagctgc ggaaaaagga gtggtatacc tgccgggcga ggccttttac ccggatggta
     2101 cacagggaga aaactatatg aggctcaact ttactttccc gaattcaaat aaaattgaag
     2161 aaggaataaa actgctaatg gatgccttga cagaggttga ggtaagagac ggatcgaact
     2221 ccgcgggcag aagctattca agaagcccga tagtgtagga ttgaaaataa aaatgaggag
     2281 ggttttattt ggaaataaaa attaaacagg tggatgcctt taccggaata ccctttgggg
     2341 gaaaccccgc cggggtggtt actgatgccg caggcttaag tgatgaattg aaacagaaaa
     2401 tcgccaggga aatgaatctt tcggaaactg cattcgtatc cccttcaaag gccgccgatt
     2461 ttaaagtgca attcttcaca cccagatttg aagtggactt atgcggccat gcaaccatag
     2521 gcactttttc ggctcttcat gaagaaggaa agcttccttc cgataagaac atattttacc
     2581 aggaaacaaa agccggaatc ctgcctgtgg aattaacaag catcaataat gaaaaggtat
     2641 tcatgatgac tcaggcattg ccgaaatttg aaaacattga tctgagcaaa agtgaaattg
     2701 caggactcct aggcttgagt aaggatgatc ttatggacac accggattta atggtaaaca
     2761 ccgggatatg gtggctggta ttcggagtta aaacactgga taagcttatg aatgcaaagc
     2821 ctgatttaaa aaccattgag agaataagcg agcaatataa cattgtcggt atcactcctt
     2881 tctgtctcga agcactggac ccgaaatatg actatcacct gagagcgatt gctccttttg
     2941 tcggagttgc cgaagatcct gtatgcggta ccggcaacgg atgcgtatct tcatacatag
     3001 ttcagaacgg gctggtaaag tgtgatgata ctgtaaatat gatcggagag gaagggcagg
     3061 aagtcaacag gcccggctgc gtatatgcaa gcatttccaa gaaggaaggg gaaataagcc
     3121 gggtacaaat cggaggtaca gcagttacaa tactgcaggg agtaataaaa atcacagatt
     3181 agaaaggggg aggatattca atgaaaacta tcggaataat cggcggagta agttgggaat
     3241 ctactgtcga gtattacagg atactgaatg aaaccgtaaa taaaaaactg ggaggactcc
     3301 attccgccaa gtgcctcata tattctgtag aattccagga gataacggat ctgatgcaga
     3361 ccggggactg gaaggctatt tcagttcgga tgcaggagat agcaagtata ctgaagtcag
     3421 ggggagcaga cttccttatc atatgctcaa acactcttgc tgcaccggct ccggaaatag
     3481 aaaaaaatat cggtatgcct gtgcacagca ttgccgaagc gacagcaaag gaaatactcg
     3541 gaatgaagct taaaaaaata ggcttgctgg gaacaaagta taccatggag ggtgattatt
     3601 acaggaacac tctttataaa tacggtatag aaaccgtagt accggagaaa gaagataggg
     3661 aagcaattaa ctcaataata tttgatgagc tttgtcttgg catattaaag gaatcctcca
     3721 aggaaatatt cagaaatatc ataagcaagc ttcagtcaaa aggcgctgag ggcatagtcc
     3781 tcgggtgcac tgaaatcccc ttgcttgtaa agcaggagga tgtaagcata ccggtatttg
     3841 atacactgac tatccatgcg gtttccgcag cagagctggc actaggctaa ggaacaaaaa
     3901 ggacacgggg acgtttctcc tgtcccgctt tcagcgggac aggagaaacg tccccatgtc
     3961 ctcaagatgt gtcgcattat tcttcttttt tcccgcactt gtaaatgtct tttcttctat
     4021 gtgcaagaag gggaagctcc cttctcacct tttccacata atccagattc aatgtctcaa
     4081 taagcagccc ttccttttca tccaaacgtg caaccacctc tccccaggga gaaactataa
     4141 tggaattccc gtaggaagta taacaggaat ccacatccct ggccggtgca gttccgattg
     4201 tgtacacctg attgtctata gctctgctcc tgaacattac ttcccaatgg gccggtcctg
     4261 ttgtcatatt aaatgcggca ggtgctatca acactttggc gccttcttcc accatcagcc
     4321 gcgacagctc cgggaaacgg aaatcatagc agacagccac gcccattttc ccgaattccg
     4381 tttcaaacac agtgacagta ttccctgccg tcaaggtttc ggattcctta aaatgctgtc
     4441 ccccttcaac tgctatatcg aacaggtgca ttttcctgtg cttcccgatt ttatttccct
     4501 tacggtcaaa tacataacag gtattgaata ctcttccctt atcgtccttt tccggcatag
     4561 tccccgctat aagataaatt tgatattttc ttgccagctc cgaaagcatg ccatagcatt
     4621 ctccgccctc ttcctctgca tatacgggga agttggaggt tttgtaaggg cagttgaaca
     4681 tctcgggaag tactgcaata tctatattct gctctgctgc ttttctaata aagtcagatg
     4741 ctttgaccat atttctcagc ttgtcttctt ccacatgcat ttgaatcagt gcaaccttgg
     4801 gtttgttcac gcattctccc tccttataat actttgacca ttctatgatt cttattataa
     4861 ggtttaaaga atcccaggtc aatttccgtc taacgcttta tctatttttc taatgtattc
     4921 cgctgcatat gcatcctcaa gaagtacttc attataccat ggcttatgaa tatcccggct
     4981 gtttccgaag taacgggctt taatcagaat ctccggccgg tattcaaaat gaagtatatc
     5041 aaaatgcccc cattttccgc cccatataaa attgttcctt tcaaaaagtt caacaatctc
     5101 attgggatag gaggacagcc ttttttgccc ctcctccctg gaagcccact gccaatagtc
     5161 cctcttatct cttgcaaggt ctatggctat accgtatgaa tgagggctca ggcggtttgt
     5221 acctgaaata acgcgataat tgtaggtgcc gctgcatgga tatacacaag ctctgatttc
     5281 ttttctgttt tttgctgctg aagcaagttc atccataaca ttcttaaaag cttccccagc
     5341 cttattgttc ctattgaact gaaacgctcc gcatccggta tttacattta caaggtttcc
     5401 ttcaatctgc tgccttgagc ctccataaac ttcattcagc aggctgtata ccctggcacg
     5461 ccccggatca taatttatgt ccataagctt ttttacaagc ccaataggat aaacgggctc
     5521 catcatatcc tgcaaatcag gatatgcaat ttttgcctcc atgtccttta tttttttgtc
     5581 gtcatataat atcttccttc cggacttcat tgttacatat acccggtctt ccagcttctc
     5641 tatacttgca atgtgctcag gatatgccat catcaggcaa agaagatcct gcttcatagt
     5701 aatgtcgtag gatgactcga ccggttcaat ataagtgaag cacggctgcg ggttgtttgc
     5761 ggtatatgca aataccgtaa tcatacaaag tattgaaact aaagaaataa ccttttttgt
     5821 tttaccgccg aatccatatg aagcaaacag cagcctgaag ataataaaca tggacagtgc
     5881 cccgaacaac ggataaaaga agcttataag ccctttgaat tccagaaaag ccaggggcag
     5941 gcagcaaaga acagtgaaga aacaggtttg cttatatgta atatgcctgt tcttactcag
     6001 cctttttgct atgctgaagg catttgatac tgcagttgaa aatatttcac accatatgca
     6061 aagggcaaca acacttttta ttatccttcc gaggcctgag gttatgcagt ccatgggtat
     6121 cgaataattg aacacttggg ggctcttaag agtaaggctt atattgagca taagggacaa
     6181 aagcataagc cccattgcgc ctaagaaaac tccggttttg agaaccggca ggtttttaac
     6241 tttctcaggt attgcagaca gcaccccaat ggaaagaaaa gtattgtaac tgcaaaaaaa
     6301 cagaaaatag aataccggct taaaaaagtt gccgtcatag ttctccgaaa gccttaaacc
     6361 aaaacccccg atatcgccgt tcataatagt tgctgccagt accgcagtaa tgacagcgaa
     6421 aagtatcgga actatgacgg aattaatatt aagtattcct gtcagtgaat gaagtattat
     6481 gataaatgta agaactgcca tgagtactac ccctaaggta tggggcaatc ccaggctttc
     6541 atgaaaaagc gcgccgctgc ctgcgaacat tatggaagtg cctatgaaaa gaaaggcagt
     6601 taccagtata tcatatacaa atccgattct gtttccggca acttgatata tgagttcctt
     6661 gtaattgtag gcattgtaat aggctgcagc ccttataacg gcagcagcca ttaaataaaa
     6721 aagtatacct gacagtaata gagttacagc gcctatttgt ccaaagcagg taaaaaactg
     6781 catgatttcc tgacctgtgg caaaaccggc gccgaccacg gtgccgatga aaactgcacc
     6841 tatctggagc gaaagcttaa aattcttaat catagaaaat ctccttttat ggaatagtca
     6901 tatctataat tatttaagct ttcactgtcc tatgaataaa atcactccat ctgttccaag
     6961 gttcttatca ttctatacaa agtatcgttt acaggtgtcg gtactccata cttccttcct
     7021 attttccgca atacacccga gaacatctcc acctctgttt tcctcccggc ttcaatatcc
     7081 tgcagcatgg aggtcttgcc ctccgctgat agggtatcca ccagtttatg cacctctttc
     7141 atgctttctt cctcaagatt gatgccggct tttatcgaaa catcgattac ttccttcata
     7201 gctgcatcca caagctcgcg gatttcctga acggtattga acaccctgaa tggcgctccg
     7261 agaactgcag aaacctgatt cagacccacg ttcaccatat atttccacca taaagccttt
     7321 atcatatttt ctggaatatg ataaggaata cccgattcat ccaacagaac ctttacacgt
     7381 tccacccttt cggtatgcag cttattttcc cgctcaccaa aatgcagctt cccaaagctt
     7441 gtgtaacgaa tattattgcc ttcccttagc gcatcaatgc ctacacagga agaatacaga
     7501 agcttttcca taccgaaggc ctcacctata atctcttcac tgtcaattcc gttcataaga
     7561 gacattataa cggtatccgg ccctacgcgt tttcttattg catcaacagc ctggctgaga
     7621 ttgttgtatt tgaccgctat aagcacaaga tccgcagggg cctcatcact atccggctgt
     7681 atgaatttta agtcacaggg ctttccgttt actgtcagtt tgttggtgtt gtatttttca
     7741 atcctggatc tgtccgccac aacagtaaga ttaacattat tgttttggta aagcctttcg
     7801 gcatagctgc ctcctatggc tcccaagcct atgattgaaa tattcgttat ttttttcaag
     7861 ctatcatccc ctttccaaac atcccggtac aatctctttc ccttcagaaa agaaaaacct
     7921 gatatggaaa ttataccaca tcaagctctt tcattatata tgcataagcc gaaaatgccc
     7981 ctgtcccttt attatccaat cttaagaaac tcccttatag agatggagta ttgcatttcc
     8041 ttcatcaatt ctttttccgg cttaaatcct actgacttaa atgcccttat tgatcgttca
     8101 ttctttaagt taatttttgc aattacctca tccatcctcc actcaaaaaa tgcatggtgc
     8161 aagccttcca taattgcatg ttttcctatg cccctgcccc atttttcctt ctcaccaatt
     8221 actatgacca tctctgctgt cttgcccttt ggaacaagtc ttaaaaaacc tatgggctgc
     8281 tttttttcca taatcataaa aaagctcccc ttttgattga ataaatgagt aagaataggc
     8341 atgtttactc tttgaattgc ctctgtaatg ctctttacaa cattccgtcc ctcgtttaaa
     8401 tatttaatca cctcataatc atccagccaa tcagcaatct tccatgcgtc agcttgatag
     8461 acctcctgcc gcagctggat actgccagcc ttcattaaca caaccctcca taaaaaatta
     8521 taagcctaaa atagtaaatc tattataggc tttccctaag gttatggtaa tgataacatc
     8581 ctgttacatt taagtcaaat gctgtaaacg aagttgcgag agtttaactg ttgatattga
     8641 ccaaaagagc catttccctg actttcccta cccattccga aataagcgcc tcacgctgta
     8701 ctccatccct tgtatccctc ctatacaata aaaacaactg tggcttccgt caccacagtt
     8761 gtttttttgc ttctcttttt tatgcgaatc tttttctcat cctgtcccat tcaatatccc
     8821 tgttggcctc aatctccctg taatcgcttt catccagatg cctgaacctg cgctgcagct
     8881 tcaggtattc ttccaccggt ttgaattcct tgggtacaaa attgaatctg aactcgttgt
     8941 tttcaaactc tgcaagatac cagaggccgc attcaacagc aagctttgct gtttttaccg
     9001 ttatagcggg gtcatagccc cacccggtag gacatggggt cattacgtga ataaccttgc
     9061 agccctttat attcttggcc ttttcaacct tttccataaa atccttcgga taagcaacag
     9121 aagctgtagc cgtgtaaggt acacgatgag ccgccatgat ttcaaacaga ctcttcttga
     9181 acttgttctt ctcacccttt gagcatgagc cggtcggtgt ggttgtagtc catgctttgt
     9241 atgaggttac gccgcttctc tggtttcccg tgttcatata tgcttcattg tctacacaga
     9301 tatagataat gttctcattt ctttccgctg ccccggacaa tgcctgcagg cctatgtcaa
     9361 ccgttcctcc gtcccctgca atgcccagaa tggtaatatc ctcatcccat ccgagagctt
     9421 ctgctgccac tgccattcct gataatgtag aggcaagggc cggaaatgca gttattgcat
     9481 tgtttacaaa aattgaagac tggggatata ctgttgtggt tgctgcaaaa cagcaggcgg
     9541 gagtggccag acaggttttt tcgcccaata ctttcagagc cagcctggct gcaagcgtta
     9601 ctccgcatcc ctggcaagtc tttgcaccgt acaacaattc ctcatcaggc aagtttttaa
     9661 aatttactgc cattattccc acctcattcc taagaactgc acttcggctt cattctttcc
     9721 agaagccata cctaaaagct tgctgaacat tgtctctatc tgatgtttcg gtatatccct
     9781 tccgcccagg cctacgatgt aattggccat ctttgcgcct gaaccgtaca tggcagactt
     9841 gacctcagaa aatacagttc cgtcaaaacc gaaggatatg cttctgtcta ttactcctac
     9901 agctttaaat ttatctctta tagattcgaa atactccttt gggaagggcc tgaagcttct
     9961 gagctttata aggcctacct tctttcctgc ttctctcatt tcgtccaccg ctactcttac
    10021 agtacctgcc acacttccca ctgccaccag gactgcctca gcatcatcgc agcggtattc
    10081 ttcaatcatt ccgccgtagg atctgcctgt caattttgca tattcatcat tgacagagac
    10141 tatctttctc tttgcagctt caaaagcctt ttgctgcaaa aaccggcctt cggtatggaa
    10201 atcagggccc actgacatac ataaggtttt gggatccttc atatccatct tgtttgttgt
    10261 actgtaaggc ggcaggaagc tgtccgcttc ctcctgctcc ggtatctcca ccatatcata
    10321 ggtatgtgta agggtaaaac cgtcgatatt gatcatgaca ggggtcatga cttctggatc
    10381 ctctgctatc ttgtatgcct gtatgacggt atccaatgcc tcctgtccgc tttccacgta
    10441 cagcattatc catcccgaat ccctcattgc catagcatcc ctctggtctc cgaaaatatt
    10501 ccagggggtg gcaacggaac ggtttgcatc aaccattact atcggaaacc tggaaccgct
    10561 gacatagctc agcatttcgc acatgtaaag gagcccctgg gaagaggatg cagtaaaggt
    10621 tcttgctccc atcatggacg cacccaaagc tgaagccatg gcgctgtgtt cactttctac
    10681 catcatatat ttgaaatccg cttctccgtt tgccagaaaa tctgcaatct tctcaaccaa
    10741 agtggtctgc ggtgtaatcg gatatgccga tacgacatga ggccttgaaa gctttactcc
    10801 gtaagccgct gcctcattgg cggacaaaaa agctttttta ctcattccgt atcaccgtcc
    10861 ttaatcattt taatgcagtt tttcgggcat tcggctgcgc agataccgca tcctttgcag
    10921 taatccatat ctatttcaag atggtcaccg ctcttgtcta tgcagccttc cgggcaaaat
    10981 acccagcaca tcatgcattt tatgcacgct ttactgtcga tttccggatg caatgtcctg
    11041 aaacccgaat ttggagtcgg cagtatccct accccattgc ttgatccaaa aaacatttca
    11101 tcccaggagg atggtgcttt ataatccttg ttccatttcg gtttgttcat tattgcatca
    11161 ccgcctcctt aaccttgtca taagctgccc ttgccgcttt cttgttcttt tccctgagat
    11221 tcctgcccat tacatcgtca atggctttct caaccgattc cattgatacc atttcggttg
    11281 cgcctactgc cgcaccgagc atgattgtat ttactatggg attccccaaa agttcaaggg
    11341 caatatccgt ggcgtcaaga acaactattc tggatggatc ctcaaatccg atatgtcgtg
    11401 ccgcttcatc cgctgttttt cttgtgttga taaccaggac accgtcgggc ttaagtcccg
    11461 cagccacatt tgtcacatca cacaaagtct catccagcac aattacgcag tcacattcat
    11521 aaacctggct gtggtcggta atggttttgt ccgctattct ggtaaaacca agcaccggtg
    11581 ctccgcggcg ctcagggccg aaggatggaa acgcctgtgc cgatttacct tcgaataccg
    11641 atgctgcaac gcccagaagc ctggcggctg taaaggctcc attgcccccc cggccatgcc
    11701 atcttatctg tttcactttt tcttttcctc cttcaatggg ctctctacct gagccagggc
    11761 acgtgcataa tctacataat tgtcccattt agtcgctgct ttcactacca tctttgccgt
    11821 gtcaggagac ccgccgccgt gcatacagcc cggcactcct cctcctattg tcagccactc
    11881 tacaagtcta gccatcttaa tcctgtcttc tccaggtact gcagacctta atgattctat
    11941 aagcttctct ccgtataacg gtgacatcat atccttatag gaaggcatgc atcctgtctc
    12001 tgttatgcct cctgagattt cctgggccaa taccttcgta tcgtatggca aggttgccac
    12061 atgcaccttg tttacatggg caagcagcgg attgggataa aatgctcccg atttgtgctt
    12121 tttccctgta tacatggctc ctattcccag tccgtatgtt gtttcattgt ttattgccat
    12181 cttggtaagc ttgtcctgga atgccttctg tgcaagcccg tttgctcttg ccattcctaa
    12241 tgccgctcct atcatcacgt ctccctgccc tgctacacat gctcctatgg ctgccctgta
    12301 tatcgcagta aagttgccaa tcagcttccc tgtatatttt gtttctccgt tcataaacac
    12361 atgcttgctg ggtacgaata cattgtcaaa tatgatatag gcctgggtgg ttccggactt
    12421 tataccgtcc cagccttcct cttctattct tgtatccgaa ggacgccttg tctctacaat
    12481 agtcacccct tccgcatctc tgggcactgc ggcggctatt gcgtagtcac cctcatcctc
    12541 tcccattccg gtggtaggca tgaccattat atactgtgac ccaactgttc cgcatatctg
    12601 gttcttgaag cctcggatta cgactccgtc ttctcttttt tccactacat gcaggtacat
    12661 gtcgggatta tcctgctgac ttggcttaag tgccctgttt ccctttgcat cagtcagtgc
    12721 cccgcttaat gcaagagcat tattctctgc gaatatgcag aagttcttta atctttcatg
    12781 ataatccgtt ccaaattcct ggtccatgtc aaaggttaca ctccaaagtg cgttcaatac
    12841 cgtccatcct gcacatacag gccccataca gcttccgcag gtatgatatc cgtctctttt
    12901 catgtttgcg ttccccagca tgtcatctgc gcttgttatc agtgtattcc agcgatgggc
    12961 cttgttgcct gaaagaaatg aagtatttgt ataaagttgt tccttttccg ggtcgaagga
    13021 attgtcgtac cattttgcta cattccttat atgggatgct gtggcaggat gttctgtcac
    13081 atcctctata agttctcccc acttgtatac attgggcctc atcttcttca tttcctcaat
    13141 atactgtgct cctgttttta atgccattgt ataccctcct ttataaatca agtttaatca
    13201 tcaactgatc cagaatcctg ttcaggtctg cggcggcacc tgcgtccgat acctcaaata
    13261 ctgccgcctt ttcatccttg ttgatgctta ttatcaatcc ggaatccttc attccgcata
    13321 tatgatgggt cgccccggat atcccgaagc ctacatatgc cttcggcctt atggacttgc
    13381 cgctggtgcc taccattaca tgttctcccg gtgcccatcc ctcatccact actgggcggg
    13441 tgcaggctgc cgctcctccg aatatcgccg ccagcttctc aagcttcccc cagttctctt
    13501 tgcagccaat gccgtagcct ccgcagaata ctacttccgc tttgtccaga ggcactcctt
    13561 ctgtttcttt cttctcaagg cttattgcct ttaagggcag attatccgct gtaattgcgc
    13621 tgacatccac ttttataatt tgggcttccc tttcttccgg ttctccctgt acaaaaattc
    13681 cgggcttgac gcttgccatc tggggccgtg tgtcgggaca cagtatctct cccagtatct
    13741 tccctccgaa ggacggcact gcggctacca gatgttcatt ttcatctata tgcatatcta
    13801 cacagtgggc ggctactccc gttttcagct gtgccgctgc tgtaggcccc agttccgagc
    13861 ctattgccgt ggctccgaaa aggaatatgt cagggtcttc tttttgcacc agttctgtta
    13921 ttataggtgc atacagcatc gggttatata agctcagttc gtcggattcc accaggtata
    13981 ttttttctgc tccgtattcc gccagtgttt tgcatgtgct tcctatcctg ctgcctataa
    14041 gtaccgctgc cacttccgtt tctcccttga gtcccgatgc cagctccttt gcttttgtca
    14101 aaagctccag ggtgactctg tccagggtgc agttcacata ttcggcaaat acccaaattt
    14161 ttttcattgc cgggcacctc cctctatgat gcatgcatca ccttttgtgg ggatgtttac
    14221 tcctgctgtc ctgagttttt ctatgatggc atccgctatt tcttctgctg aaccggtaag
    14281 catttctcct tttcgcttga gatccggtgt gaatatggct cccgcttttg tgggtgagcc
    14341 ggcaagtccc agtctttctt cttctgcgtc aaggtctgct ttaccccatg ttgttatcgg
    14401 cttgtttttg gcttttatga ttcccattgc cgatatgaac cggggtttgt tcagttctct
    14461 ggatactccc agcaggcagg gcaatttaac ttcccattcc atgaagccgt tttctgcttt
    14521 ggttctgact ctgagggttc tttcctccag gttttccagg ctgaatacat tccacaaatg
    14581 gggtactcca agccattctc ccaattgtga ggatacctgg gctgtagctc cgtctgcgct
    14641 ctctgtgccg cagaatacaa tgtcgaagcc ttccagcttt tctattccct ttgccagggt
    14701 gtaggaggta gccagggtat ctgctcctcc aaaggctctg tccgacagca gtactgcttc
    14761 gtccgctccc attgccagtg cttccttgag attctccgtg gcatttggag gggccatggt
    14821 gagtactgtt actttgcccc cgtactgtgc tttaagcttc agggctgctt ctatcccatt
    14881 cttgtctacg gggtttatta ttgtcggtat tccttccctg gttattcttt ttgttacggg
    14941 gtctatgctt actttgtcat agtaatcagg atccggcacc ggcttgatgc atactactat
    15001 ttttaaagcc ataagtattc tcctttatct gtttctgctc tgcttagctc tcttatgctt
    15061 tgaatgccga tagtgtattt cccgctatga taagctgctg tatgtgggag gttcctcctg
    15121 aaggtattga tgccagagca tcccttaaga aacgtcctac agggtattcg tttattactc
    15181 catagcctcc cataaggtcc atggttcttt ttgatgctct taccgccgct tctgtggagt
    15241 agtattttgc tattgcaaaa tccggtgttg cagggagttt tgcatccttt ttccctgctg
    15301 catagtaaag aagcattttt gccgcttcgt attcggtgcg tatgtttgct atttcaaatt
    15361 gaattgcctg aagctttgct atcggctttc cgtaaactac tctttcgttg gcaaatttga
    15421 ctgcttcttc tacgcagcct cttaaaatgc ctacgcagat agcactcatt cccgctcttc
    15481 ctacttctcc tattgcggcc atgcctatct ttgctccggc tccgacattg cctatgatgt
    15541 tctcttgagg tatcttaact tcgttcagta tcacgtctcc tgtaaccgag cctctgagtc
    15601 ccattttatg ctctttgcgt ccggcaccaa aacccggtgt acccttttct attatgaatg
    15661 cactcagctg gttccttcct ttttcgtcct ggcctgttac tgcagtaatc acgtttacgt
    15721 ctgcatgatg gttggtgatg aagcatttac gaccgtttag cacccactgt ccgtcaacta
    15781 attctgctgt ggttttctgc cctgcaaagt ccgagcctcc gccgggttct gtgactgcaa
    15841 gcccgcatag ttttgttccg gcacataact cagggagata tttcttcttc tgctcttcag
    15901 tcccgaaatc caatattggc gacattccaa gatggtgtgt catgagggca atgcccaatc
    15961 ctgcggaata cctcgatatt tcctctatgc atattgctct ttccacatgc cccatccctg
    16021 ctccgccgta ctcttcaggg gcgaatattc cgttgattcc caattctccc attatgggaa
    16081 aaagctccat tgggcaatag tcattttcat cccattctgc cgctttaggc gccacttccg
    16141 cttctgcaaa ctcccgcacc gctttgcgaa gtaattcctg atcttcggta aattcaaaaa
    16201 attgcatata cactatctcc ttgttaattt tttaactata taaatcatat atagtttcct
    16261 aaaaatatga tagcattacg aattttgttt gtcaacaaat tacttttata cagcattccc
    16321 ttaaaccaag taattccaat gccttatcag gggtacactc cttaagccct catataacta
    16381 ttacggtttt tttattaaag gtccattgac aaatttactt aatttcttta ttctttatgt
    16441 tattataaat atttaaatcc ggaagaaggt tgtattgtga tgatgctgga cgaatgtatc
    16501 aactttttgc tgactaaaac ccaacaggta gtatttcagc aatttaaggc aggcttggcc
    16561 aaatatgacg taacccccgc acaatacgga gtattgaaat gcctatggtc tcaggactgt
    16621 cagaatccca gccagattgc ctgcagctta tttctggacg cttcaaccat taccggaatc
    16681 cttgaccgga tggagaacaa ggacatgata aaaagaatag cagatcccaa ggacaagaga
    16741 tccttaaagg tagtgctgac agagcgggga aggctgctgg agaaaccggt gacggaaatc
    16801 attgatgaag agaacaaaaa ggtgctgcat gctctgaatg aaggggagcg gaagaagctt
    16861 acagagtatt tgaaccggat aagctccata taggtttttt gaggaatgat gcttatatga
    16921 tatatgatga accattattc aggcccccca gtgaggctta cagcttgatt tttcaagcta
    16981 caatagggtg ctcccataat aaatgctcct tctgcggcat gtataaaagc aagaaattca
    17041 ggatccgcag caagagcgat gtattcaggg acctggagga aatggctgca cgctataata
    17101 aggcgcgcag gatttttctc gcagacggtg atgctttggt gctgaagacc cgggacctta
    17161 aagatattct gcaaaaaatc agagaacttt tcccctgttg cgaacgggtg ggaatatatg
    17221 ccactcccgg ggatatattg aggaaatccc ctgaggattt gacgtccctt aaagagcatg
    17281 gcctcgggat agtatatctg ggagttgagt ccggcagtga taaaatcctt aaggctgtag
    17341 ataagggagc ggactccaaa gaaataattg aagtgggaaa gcgcatagta agctcgggta
    17401 tcaagctttc cgtaaccctt atttccgggt tggggggaaa ggatggctgg gaggagcatg
    17461 caagagaatc cgcaagggtt attaacgaga taaaccctga ctatctgggt ttgctgacct
    17521 tgctgattga gcccggcacc cggatgcatg atttggtgca gcaaggcacc ttcaagctgc
    17581 tgtccgctga agaagtagcc cttgagactc aactgctgat ccggaatctt gagcttcgga
    17641 actgtatttt cagaagcaac catgcctcca attacttttc ccttgaagga acccttcccc
    17701 atgacaaaag caaactgctg aagcaactgg ataatattct gaacaacatt gaagattaca
    17761 gcggagagta cttcaggaga ttatgaagca gcggtgaatc agctcctaac ggttcttttt
    17821 cgcaaggatg cgcagctttt caaatctatt ttcattcccc gcaataagcc tcttaatatt
    17881 gcttctgtga gtgtagaata tgaaaactgc aataataatg ctgaaaataa gggctgtctg
    17941 atcgatatta tccgtattat taatatataa ggctgtcatt gtcacaggaa acacaattgc
    18001 tgctgtcaaa gagcttaagg atacatatcc gcttgtcagc agcacaacag caaatgaagc
    18061 tgccgaaacc aggaggggca cgggaaacaa agcaataagc atccccgttg atgtagcgat
    18121 gccctttccg cctttgaaac ctgcaaatat ggtgaagcaa tgtcctatta ccgcagcagc
    18181 acctgcgagt atcggaacca gcatactgct ttggggcgcc cctccggaaa gattcagtcg
    18241 cgatatccac tctgttgccg caaaaccctt gaaaatatct atgaacacaa caaaaatgcc
    18301 ggttttccag cccataaccc tgaatacatt tgtagctccc gcgttcccgc ttccatgttt
    18361 tctgatatca attcccttta cagctttgca tactattatg cttgttggta tggaacctgc
    18421 gagatagctg agaataatta tgactgcaat atttatcatg ctattataca atgtgctcat
    18481 tattatcctc ccaatcctta tttttcaaat gacaatattg tatctgcgtg ctttcagcgc
    18541 caccgtcttg tgtgttatcc ccaaggcttt tcccgcggcg ttataactgc caaaccttgc
    18601 cagagcctct ttaattatct ctttttcata ttcctccaga gtagctatat tacccgaatt
    18661 attcatatta ataagtgcag gggcggaaca tgtatgccgg tttacgcaat aaagcgacga
    18721 tattttggaa ggcaggctgt ttatgtcaat ttcattcccg tcagtaagag ctatgactct
    18781 ctccaccagg ttttccaatt cccttatatt accgggccaa tcgtaattca gcaggcagtt
    18841 ttcagcctcc ggggatactc cggttatttc cttattcata tttatgctta ttctttttat
    18901 aaaatactcc aaaagcatag gtatatcttc cctgcgttct tttagggaag gaacataaat
    18961 aggcagtaca tttaatctgt aatacaggtc ttccctgaag gctcccttct ctaccaatgc
    19021 ctcaagatta caatgtgttg ctgcaataat tcttacatca caccttattg tatcacaacc
    19081 gccgattctt tcaaattcct tatcctgtat aactcttaaa atctttacct gcatatccgg
    19141 cggcatatca ccaatttcat ccaaaaataa tgttccgccg tcagcctgtt caaattttcc
    19201 tgtttttctc ctgacagccc cggtaaaagc accccgctca tagccgaaca gttccgattc
    19261 caagagagta gacggtatag ctccgcaatt cactattgaa taggggccct ttgccctgct
    19321 gctgttatta tgtatggctc tggctatcag cccctttcct gttccgcttt ctccgcaaat
    19381 caacacattt gagcttgtct gagccgcttt gtaagctatc ttaagcgctt tcataattga
    19441 actgctttct ccgactatat ctttgaacat agttcttata ttattcatgt aattaacggg
    19501 cggtgaattg aattctatta cagtatcttt tttcctgcaa atatcctgtg cagcttcttc
    19561 cctgtaaata gctactactc ccttaaattc accgtttgaa tatatgggag aagcggaggc
    19621 gcttacatga gcagcaccct tataaaggac aagatgtccg catatacatc ttttctccct
    19681 gaatgcagtt aagagtacat cgtcattctt tgtcagaaaa atattcatcc ccgtttctat
    19741 gcggtcactt atctttagta ttctttcgta agcgccgtta aagtattcga tatagccccg
    19801 ctcatttatt atacagactc catcagtatg gtcgtcaaat atataaaagc tttcggcacc
    19861 taaaaaaccg tcctttgctg ccttaccggc tttgagactt aagctggcaa ctctgttgtt
    19921 cttcacattc aaccctccct tcattactaa atttggttaa acagttcttg aagaaatcac
    19981 atttgcgccg gtggaaagga tattttcgat tataactgct ccaagtgcta ttggagtatc
    20041 tacctccact gaatttatta ctttcataca atcaagcagt aaattctttg gcacaggttt
    20101 atccgtcctt accggcaatc tcctcaaaat agcattattc agctttactg ttgtggttaa
    20161 tattctggcg ggattagtaa cttccgcaat ggcataccca atacccctat tgcatctgtt
    20221 ccctttgacc aggtaacccg aatcactgct gctatcccca attacctcca agctgcagcc
    20281 tataggacaa atggtgcatg tcacaatatt cctctccata tacatcctcc ctcactcaac
    20341 atctacggtt aacgataaat cctccgctgt cttcaagctt tcagctgtaa tgcatatacg
    20401 ttccatttct cccggtcgca tatatgactt cttaattatt ttgattacag tgtctccgaa
    20461 ttttaccacc agttttttat tcgtgaatat tttgttcaca cgcatgaaca gatcaacaga
    20521 ttgctcaact ttatccttcc tgataaaatg gggaactata tacctgattc cattgcccgg
    20581 ttttgtcctt atggcaggac cgtacccgcc tgtttcacca tttacaaagc gggcagcatt
    20641 tcttcccgct atccggcttt cctcagtcac ataatcaaca agatcatgga catgaactgc
    20701 gtttccgcaa gcgaatactc ccttaaccgt tgtttccatg gattcattta cgacagggcc
    20761 gccggttatg ggatccagct ccacgtccgc ctctttggaa agttcgtttt cgggaatcaa
    20821 gcccactgat aacagaaggg tatcacactc ataaaactgc tctgttcctt taatgggttt
    20881 cctgttttca tcaagctctg ctaccgtaac cccttctatt ctgtcctttc ctttaatatc
    20941 aacaattgta tgatttagga gcagcggaat gccgtagtcc tccaggcact gaacaacatt
    21001 ccttatcagc cctcccgggt aaggcagagc ttcggcaacc actttgatcc gggccccttc
    21061 aagggtaagc cttcttgcca taatcatacc gatatcccct gaaccaagta tcactacatt
    21121 tctgcctacc atatagccct ctttattgac aaaccgttgt gccgtacccg ccgtaaatat
    21181 gccgcttggc cttgttcccg gtatatttat tgcccctctg gttctttccc tgcagcccat
    21241 agccaatatt gccgcccttg ccttaatatt gacaaacccc tctgttttat tgaccatgct
    21301 gataaccttc tcttcggtta tgtttagggc cattgtgtcc agtttgtatt ctattcccag
    21361 ctccttgaat tcttttatga accgttcggc atattccgtc ccggtcaatt cctccctgaa
    21421 tatatgaagc ccgaatccgt tgtgcacaca ttgctgcagt atgcctccca gttccttttc
    21481 cctatcaatg agcaatactg ttcctccatg tttttttgct tctattgctg ccgcaagtcc
    21541 tgccggcccg ccgccgatta cagcaatatc atactcattc ataacaaccc ctccctaaac
    21601 tttccctgat gctgcagcaa ggtcgtgaac atcctcatat aagctgttat ccttggtttc
    21661 tcctgtcaat atatatgatt tggggctgtt ttttacaacg tctttgatat caatgtccaa
    21721 ctcccttgca agtatttcca tcactctcgg cccgcaaaag cttccctggc accgccccga
    21781 acccggcctt acccttttct tcacgccatc cagggacctg gcacctgcct ttctgtgaat
    21841 tgcatcaaca atttcccctt ccgttatgct ttcacacctg catattatcc tcccatatct
    21901 ggggtcctgc tttataagca aatttttatc cgcctgggaa agctccataa accttacaac
    21961 ctttcttctt acaggattaa acccgagatt ttcctcaagc cctcccatta atccctttac
    22021 tatatccgct gcatgtacgg caattgcggg agccgctgtc agtcccggcg attcaatccc
    22081 tgcgacattt ataaacccct ttgcctcctt ggattcacct atgacaaaat catctgtact
    22141 tgatcttgcc ctgattcccg caaaggccgt aatcactgca ttgggcttca ggcctttcgc
    22201 tgaccttgcc gatgcctccc atatatatga gatgccttcc ggtgttgtct gtatagcttc
    22261 cttatcctct atatcctccg catccggacc gattaaaata ttcccatgga cggtcggagc
    22321 tatgagaact cccttgccaa aatccgaggg gcattggaat ataactgtat ttacaaagtt
    22381 tcccaggctt ttgtcaaaca agttgtactg ccctcttctg ggcagtattt taaagcttgg
    22441 agcagctacc atttcgttaa ccttgtctga atacagccct gcacaattaa ttacaaatct
    22501 tgcttctgcg agtctgtctc ccatgtgcag cctgtacagg ccattcattt tctcaatttt
    22561 ggttacttca ctgtccaaaa aaagctccac tccgttttct actgcattct ccgccagagc
    22621 tatagccaat tcccacggcg aaactatgcc ggcattccgg gcatacaatg ccccgactac
    22681 ttcatcgctt aaattaccat ctatctcctg tactttatcc ttaccgatta tctccatatc
    22741 aggcacccca ttagcgatgc cctggtcata aagcttttgt attgtcttca tttcctccgg
    22801 gctgaaagcc aaaacaaggg atccggtctt tttataaggg acatcaagct cactgcacag
    22861 cttttcatac attaaagccc ccggaacatt aaaccttgct ttgagagttc ccggctttgc
    22921 gtcatatccg gcatggacta ttgcactgtt tgcctttgtt gtgccgtctg caacatcact
    22981 attcttttca atcaaaagga ttctgacatt gtaccttgat aattcccttg caatagaaca
    23041 gcctgtaatt cccgcaccga taacagctat atcatacatt tcagttcctc caataccagt
    23101 accttgcagc acctaatagc tgcatgatac ttgtctgttt gttccactac agcgttgctg
    23161 tcaatcgccg tagtcaccgc tacgcctctt tccagcgcct tgcattggaa gcaaatatcc
    23221 tgcgtataat aatgcagcaa tcagatgtct caggtactaa gaattctgat aaaataaagc
    23281 cgcaccagtc tacacctatc atcccatagg ttagcttgtg cggctctcca aatcaaaagc
    23341 cctcaatatt tacattaaat tctatgctat tacatcatta gaatatacca aaatggcata
    23401 aataagatta ttcttcccac ttaagggttc tgctgacagc ctttttccat ccggcatact
    23461 ttttatccct tgaactgcta tccataacag gctcaaatac cctgtcaaca ttccactttt
    23521 cggctatttc atccttgctg tcccagaatc cgacagcaag gcctgcaagg tatgcagcac
    23581 cgagtgctgt ggtttcggtt acttcaggcc tgtaaatcgg tacaccgagt atatctgcct
    23641 ggaattgcat caggaagttg tttgcaacag cgccgccgtc aaccttcagt gcctggagat
    23701 tgatctttga gtcttcctgc attgcttcaa gcacgtctct ggtctgataa gcaattgact
    23761 ccaatgttgc tcttactata tggtttctgt tggctcctct ggtcagtccc aaaatgctcc
    23821 ctcttgcgta catatcccaa tagggggcac ccaatcctac gaaagcggga acaacatata
    23881 ctccgttggt atccgcaact gcggctgcca tatcttcgct tgccgcagca ttgtcaatta
    23941 tcttcaactc atctctaagc cactgaactg atgcaccggc tataaagatg ctgccttcca
    24001 gagcatattc cactttgcca ttcaagcccc acgctatggt tgtcagcagg ccattacttg
    24061 atgaaactgc cttctcccct gtattcatca gcatgaagca gcctgttcca tatgtattct
    24121 tagccatacc gggcttatag catgcctgac cgaataatgc cgcctgctgg tctccggccg
    24181 cccctgctat cggtatttca gcgccgaaga tttccttgtc ggtttctccg tatacaaagc
    24241 ttgaagactt tgcttccgga agcattgctt cgggaatatc cagttcctcc agtatttcct
    24301 tatcccaagc caattcatgt atattaaaca gcatagtcct tgaagcattg gagtaatctg
    24361 ttacatgtac ctttcccttt gtcaggttcc atatcagcca tgtatctatg tttccgaagc
    24421 acagctcccc attttttgcc cgctctctgg ctccttcaac attatcaagt atccatttta
    24481 ccttggtgcc tgaaaaatat gcatccacaa caagtcctgt cttattcctt attgtttcag
    24541 ccagcccctt tgccttaagc tcatcgcata taggtgctgt cctcctgcac tgccatacta
    24601 ttgcattgta tatgggcttt ccggtacttc tttcccatac tacggtagtt tctctctggt
    24661 tggtgatacc tatggcagct atatcgtcag ctccgatgcc tattttgtcc atggcctctc
    24721 ttgcaacacc gctctgggta ccccagattt ccataggatc atgttcaacc cagccggcct
    24781 ttggatatat ctgagtgaac tccttttgcg ccacgctgac tatcttgccg tcattaccga
    24841 atataatcgc tctcgagctt gtggtgccct ggtctaatgc cagtatatat tttgacatat
    24901 gtgttccccc tttattttaa ttaaataact ttacagaggt agttatatta tatcattacc
    24961 ggaataatcc catatacaaa aatgtgccta ttatcgcgcc gacaatcggc ccaaataccg
    25021 gaacccagga ataactccaa tcatttccgc ctttcccggc tatgggcagt atggcatggg
    25081 ctatacgagg cccaaggtca cggcaaggat tgattgcgta tccggtaggc ccgccaagcg
    25141 aacagcctat tgccgtaatc attgctgcgg ttaccatagg cccaagtccg tctgcaagct
    25201 tattggcacc aatagccagg cacataaatg ccaggacagc agtaactatc atttccgtca
    25261 ggaaattctt cccatagtcc cttacagcag gagccgtaca gaacacaccc agctttgctg
    25321 ccttatcttc cgttatcgcc cagtgcggca aatatatcag ccatactact gctgcaccga
    25381 ttatagctcc aagcatctga ccggctacta ttcccggtac caatgcccaa tccaatgttc
    25441 cggctacagc attagctatg gtaagagccg gattaaatgc tgttcccggt gctgccaaag
    25501 cagccatgcc tacagcaagg ccccagccta ccgctataac aatccagccc gatgattgtg
    25561 acttggactt gttcagtgta actgccgcac atacgcctcc gccaaatatg atgagaatca
    25621 ttgcacctat gagttcactt aaaaacggtg acataatatt taccccttcc ttcattataa
    25681 tctcctgtcc attttccaga gttgatattt ctgacccccc ttctccctta ttttctccgt
    25741 cgctgccatg acgtgaataa taacggcctt acatgtacca aagctccttt ttactggtgg
    25801 atacccccag tgcaccggca ttcaggcaat caattacatc ctctttatct cttatcaagc
    25861 cccccgctat taccggaata cttgtttgac catgaacctc attaattacc ttcggcatta
    25921 ttccgggtaa gatctctata gcatccggtt ttgttgtatg caaggattct attccgcttg
    25981 ccagtgacag cgaatccagt gcgaagagcc tctggattat gaatatgccg ttgttttttg
    26041 ctattctaac caggttgctt tttgtggtaa taattccatc cggctttata ttgtcatgaa
    26101 aatatcttaa ggacacagca tcttttgaaa aaccttctat aaggtccatg tgtacaaaaa
    26161 cacccttacc ggcgctttta aacctgtcaa caatacctgc tatattcgaa atgtctccct
    26221 tcagcaggaa aacaatattg cagggtgatt ctgcggcaag ctcgatattg ttcaaatcat
    26281 ttattgctgc aattattggg ttcttataaa ttaatggaaa gaatttattt gacataggaa
    26341 tgcctcctcg tcttatgtat tatagcaaca ggcatgccaa aggaaaaaag cgctgtttta
    26401 ctagttttat tagtgatatg tggggcttag ttgataaata ttaccaggat ttttattgtg
    26461 atgataaaat atattgatag aatgtaccaa atagaattgt atttttgaaa taagttctct
    26521 aaaaataaaa aggcaaccta ataggctgat gaggttgatg aaaaaccttc gacataagaa
    26581 cagtaaatga gacacgggga cgtttcgcgt gtctcaaaac gtccccgtgt ctcaaaaaaa
    26641 ttgatggctc taattccgct caactaagaa aaacctaacg ctgtcaaagg tcgtccgtga
    26701 ccgtgacagc ttgctcggcc tccatgcctc gcatacggtt gtttctaagt cgagcgagat
    26761 ttgagccatt aacaatttct gtataagtat gctctgacta tacattactg ttcctatttt
    26821 gaactttgtc agcaatttga gcagctaatt aagctgcctt gctttatttg tgctatttca
    26881 catattttat atagtctccg taaccttcct cttccatttt ttcaacaggt ataaatctaa
    26941 gtgaagcact gttgatgcag taccttaacc cccctttgcc cttggggccg tcatcaaaaa
    27001 catgccccag atggctgtcg cctacccggc tcctcacttc cgtacggtgc ataaaatgac
    27061 tgttatcctc tctttcctta agcactgctt ggtcaatagg gcgggtaaag ctgggccatc
    27121 cgcagcctga atcgtatttg tcacttgaaa gaaacaaagg ctctcctgtt acaatatcaa
    27181 cataaatacc tgccttatgg ttatcccagt attcgtttct gaaaggcaat tccgtagcgt
    27241 tttcctgcgt aacactgtac tggtcatcgg atagggattt ttttatttca tcccgggagg
    27301 gcttctcata caatgccggg tccaccttcg gccgggacac agccttcagg gaagaaaagt
    27361 ctatatggca gtagcccccg ggatttttct tcaaataatc ctgatgatac tcctccgcag
    27421 gataataatg ctccagcttc ttcacctcgg taacaatcgg caccttatac ttctgctgct
    27481 ctttggcaat cgcttgctct atcgtctctc tatccttttc atcggaataa taaattcctg
    27541 tgcggtattg ggttcccacg tcagggccct gcctgttcaa aattgtggga tctattatcc
    27601 tgaaaaagtg ttccagtatc acttccaggc ttattttttc cgaatcataa attacatgca
    27661 ccgtctctgc atgccctgtg tgaggaatgt catgataaga gggattctcc gtccttccgt
    27721 tggcatatcc cacagaagtg tccactactc cgtctatacg ggaaaagtat tcctccacac
    27781 cccaaaaaca ccctccggca agccatattt ctttattcat aaaatcacct taccttttca
    27841 atacgttcta atatgtcagc aagcagcggg gacggttctt tctgcttttg tacatttttc
    27901 gctttgttat tataatacca ctttttcgac agtcttagcc aaacttaact ttttacctgt
    27961 aaacgaaaca gcaggcagtg taagtgctgg atgcaaaatt tcctgaaatc tgattctcct
    28021 tgatcatttc cttgataaaa ttcttttgta ctgttgatcc cataaaaaat actcctttct
    28081 ggcttttatc tgattcttgc agaaaggagg taactttttc actcacccac ataattattt
    28141 acattaccta ccaatagacc aatacaatct ttttgatgga atagcatcta cctgtaatac
    28201 ttcctttttc aacttacatc catcaaacag ggtattgact ttggattcaa ctcattctct
    28261 attcttacta tatcactttt atcagccttt tcgtccagct tgattcttac ttccttgaat
    28321 cgttctgtca tctcagaata catttttgtt aatagtttaa agactttatc ttccatatat
    28381 atcatgcccc tttagaatat tataacatat tttttagtgt agtcaaaaaa tggtttttta
    28441 tcctatagtc tattgggtta taccactgta atctttaacc ttcctgcctt ccacattggg
    28501 tgctgcagca ataaagtagt cttatattcc tgggcagaac gtcataataa tcctccatat
    28561 ctaaatgaga tattttcctt cttgtttctt ccggcagctc attaatagcg ctttcgtcca
    28621 atatcatccc tgtctctttc ttatcaaaac atatcctttc atccaccaat tcaatcagct
    28681 ttgcgttatg agggcaggct tcctgacatt tcatacaacc gacaattgca ttatgccata
    28741 ggggacttat ccactccggt ataggggtac catactcgtt gaaatttgtt atgcaattct
    28801 gcgcatgtat aagaaagcgt cccttatcaa tcgcacctgt agggcagtta tcaatacaag
    28861 cagtgcaatt gctgcagctt tccatcacct ttatgtcacc ccagctgtct tcttcaaatt
    28921 cgtaatcagt aacataagcc gacagcctta tataacttcc cagtccaggt atgtagcaaa
    28981 cattatttct gccgtacctg cccaagccgc ttctcacagc aataagcttc agcggtatga
    29041 caagtttgcc aaaactgtat ccttctactg caaggacatt attcagtatg cttataattt
    29101 ggttattgat gccagaatac atgtaagtag gaggaatcac tgccataacg gttttgtcac
    29161 cacattggaa ttcgacaata ctcttacgct gcggagaagc aattaccatt actgatcttg
    29221 cattgctcaa tacacttttg taatcataat tgaaaactgt aagactcttc gtgtaaaatt
    29281 ctttatccag agccccctgc tcggaaagta ttttgaaatc actgctgatt tccttcaaat
    29341 gctccgcatt aaagacctta accctgaagc ccgccgcttc aatttctgat agtaaatttt
    29401 cttttatcat attttccccc ttcttgtaac ccccaatttg acagaatccg gttacactat
    29461 tataataata atatcatagg gttatgtaac tgtcaatata tgaaatggag gttacatcat
    29521 ggaattttta tgtttggagt ttgtaaacag cagctggtat atcacccata aattattttc
    29581 agatccatta atgaataatg aatggttaat aaagcttttt gataaatgga atatgcagcc
    29641 attgattgag cccaacgagc ttgagctggc aaaacttatt gatatgagaa gccgttttat
    29701 gaagctcttc acaaaaatag ttgaaggaaa agaccttgca aaaaaagata ttgagctaat
    29761 caacagttat atggctgata taagcttttt cagacagtta cagacaaaag aaggaagttt
    29821 gggactgtat gatatcccga aaactcgtaa ttggaactgg tttatggctg aagtatcagc
    29881 atcctttgca agactttact cctcagaggc cataaataga ttgaaaatat gccagaatcc
    29941 cgaatgcgga tggttcttta tagatgacag taaaagcaga aacagaaaat ggtgtgatga
    30001 cacttgtgct tctcttatga aggtaaggcg attcaggcaa aggcaaaagc gataaatcgg
    30061 cacgccctcg ctctattgca gcattggctg cttaaatatc ttgctgagaa gtggaaacca
    30121 aaaggcctga tataggaatt tcccacaagc agcggggaca agcagcgggg acggttcttt
    30181 ctgcttttca gaagtgtcca ctactccgtc tatacgggaa aagtattcct ccacacccca
    30241 aaaacaccct ccggcaagcc atatttcttt attcataaaa tcaccttacc ttttcaatac
    30301 gttctaatat gtcagtatgc tgcttatatc catcaaacag ggcattgaat tatggattat
    30361 atcagccttt tcatcaagct tgctttttac ttccttgaat tgttctgcca tctcactctg
    30421 cccttatcta acccttagca actgcattac gaagaggcaa aaggcagatt agtgcgatta
    30481 cggctgatcc tgtaataagg cttaaagcac caatatagac attgttattg taagcaaagc
    30541 ccatagctct agttagaaaa tctataactg ccctgtctgc caatccaccc aatattctgt
    30601 caacgtgctg gtatatgata aatgccagaa caggtaaaaa tgaaacaacg attttcatta
    30661 tagtgttgca tctatagtac aacatggtta caaaccatcc aaagcagaca gcccatgtat
    30721 ttaaaccaaa acgccacaag aaccctgcaa agaaggagtt atttccataa agctgcataa
    30781 cgacactttc ataagggata ttcgaaacaa gtacattatt caatataaca ttcaatacag
    30841 catccaccaa tgccataaat gccgatactg caaccaaagc aatcgtattg ccgatataga
    30901 atcctttcct cgatacatta tttgcaagca tgaatttaaa attcgattta aagcaattca
    30961 gccctgccac aaaaagaaat atcactgttg ccatttccag gcccccgaag gatatgtttt
    31021 ccccattcac cgaaaccgtg acagtaattg cagatgctat taatatgata attaagtaaa
    31081 atattagcac agctttccaa aaatcatgta tctgatattt aatcacctta tagatattat
    31141 tcatgactat cccgcctcca actaattagt taagcttata aaaagctttt gaagttctgc
    31201 cttgccaaac tccagttcca acttctccgc cagagcttca tccttgttat tcatggcttc
    31261 caatacagta gccgacttaa agcttccgat agaatcaata cctatacatt tcctgccttg
    31321 aatgtattca tctacctttg aagcttctcc tgaaactata taagcctctg acaaaagctc
    31381 ttccactgac cgcttcagaa tcagctttcc ttctttaatt atgattgcct cttcgattac
    31441 cggagccact tcctcaataa gatgggtaga tataattata gtctttggat tttcgctgta
    31501 attggaaata agttctttat agaacatatc cctgtgattc gcatccagtc ccagcaccgg
    31561 ttcatcatac aggacaatat ctgcattgga ggcaagagct gcaatggcct tgaatattga
    31621 agcataaccc gtagataagt tctttaccct ttttttcgta tccagcccga acctttccga
    31681 taaatcttta gcatattcca tatcaaaaga gggataaaat tcctttgtcc atttatatat
    31741 attccttacc ctttcatctt cgggatacag gttttgttca gtcatataaa aaatattacc
    31801 taggacccga tcattttcaa aaactgtttc tccgtcaatt aagatttcac cctctgtcgg
    31861 aaatatcctg tttgtcacca ggtttaaaag tgttgtcttg cctgccccgt ttcttcccaa
    31921 taacccgtaa atcctgtttg attcaatggt aatatcaacc ttgtcaaggg cctttgtttg
    31981 cccataaacc ttacttactc cctttatctg aattgtcttc aattccgtta ccccccacta
    32041 tcatctttac gatatcctcc ttagaaatat tcagcttcgc agcctcttcc aggagggact
    32101 gcacatactc cttatagaag ctgttccggc gcttctcgag aatcttctct tttgcccctg
    32161 tggaaacaaa cattcccagc cccctctttt tatagaggat accttcatct acaagcacat
    32221 ttactccctt tcctgctgtt gccggattta ttttatattt taccgatatt tcagtggtag
    32281 atactatttg ggtttcttcc tcaaaaatcc cctttaatat attgtcttca atggcttctg
    32341 ccagctgcag atatatcggc ttttcactgt taaaatcaat gagcatgaat tccagcctcc
    32401 tctattagtt agttaactac ttatgtaatt aactatataa catatatgat acattgtcaa
    32461 ataaaatcgt aaaaaaaagc acattcctgc aaatcataac agaaatgtac tcatattttc
    32521 ttcgtctttt tctttttact caatcaccac cgccgtaccc gaagcggtta ccatcagcat
    32581 attgcccccc tgccccaata cctcatagtc aatgtctacc cctatgattg cattggctcc
    32641 aatagtgcct gctcttttct ccatttccct taatgccgct tctctagcct caatcagttc
    32701 tccctcatag gatccggaac gtcctccaaa aatgtttgag agcccggcag cgaaatcctt
    32761 aataaagttt actccggata taacttctcc gaatactatt cccttgtact ctttaacctt
    32821 cctgccttct acattgggtg ttgtagttat aatcatccta atccctccga acatttataa
    32881 cgtattacta tgtactacta tttgtattcc cattttataa attataagta cagatattaa
    32941 agtttattta attcaacttc ggcaaattcc tttgcggcaa gcagctcctc tgcactggga
    33001 tgcctgcggt tcatgatagc ccagcacttg cccctgcagc cgaagctttc agccgataca
    33061 tttatcccct gttcctccaa aagctccttc ataattttga ttgccttttc ctgcattcca
    33121 taggttccga aaacagcagc ttttttaacc atactgccgt tcaatgtctt gataaagtcc
    33181 acagttgaat tatccggtct ccctccgtaa actccatctc caataaacag catatcaaca
    33241 ggttcagcaa tactgccttc acctatcctt tcagccttaa cccccaaagc ctcagccata
    33301 gcttccgcta ctttccccgt attcccgttt atagtatgat acattacctt gatattcatt
    33361 ttacatccct ccaaatcatg ctaatcaaac atcccaatac aaaatacgtt aataactgta
    33421 aaaagtctga tatattgctt atatatcaga cctgaatcct tggactggta ccaggtatag
    33481 agcttatact atatggaata tcctcatttc ttgctccttc tatttcagct tgcctctgca
    33541 atccaccggg atttcctcta ttacctcccc attggaaaca atataggctg tttcatgcag
    33601 gttgactaca gggcatatat gattaggaat aatctccact ttgtccccga ctctgattga
    33661 atcatgaaac tccttgttat atataattgc atgctcatca tatacggcat gtatgcttac
    33721 tcccggccat cctttaatca ggcccaggcc ttttgttgca gtaaagcctt tatttctggt
    33781 ttgcgcagta agccccttgg ctccgacatc agtaatcacc cgctccggag taggcaaact
    33841 gattacggta gtcagaacgg ttgcagcatt gcgttcaagg cttccgtatg catttccctg
    33901 ggacgcatcc atgaaaatat atgtgccggg cctgatttca gttactccgt ccagtatggg
    33961 aaaatcatgc atcaaggaag gtgtggaacc gatactgact gtttccaaat gaaagcctgc
    34021 ttccgctgcc agttgggcaa accgtagcgt cctttcctgt gctttcaaat gtatttcctt
    34081 acatgtctcc gcattctccg ccgcatagga atgaccgtca tgggagaaaa tcccttttaa
    34141 atatatgtta ctgcacttct tcaaatggtt aacaagctca acaaaggcag cttcttcaat
    34201 aacgccggaa cgctgttccc ccacttcaat ctcgattagt acaactgcct gctttttact
    34261 gccttcaaaa gccttctcta tcatatctgc ctgcgggata ctgtcaagcc cgaaggatat
    34321 atcaattgtt tcagccaact tgcggattcg gcgcagtttt tcttcaccga ctatctcatt
    34381 tgcaatgaat atatctctca gtcctttggc tgccatgact tccgcctcgc caaccttggc
    34441 cacggcaata ccttttgctc cgagttcttc ctgcaaaaga gccagcctgg acattttatg
    34501 ggtttttgtg tggggcctca gtgccacatt gcagcgattc gcataatctt gcatccttct
    34561 gcaattactt atcattatat ccttatcaat taataatgcc ggtgtatcca attcccgata
    34621 attcattact ttccctcctt ttaagccgct tcagtgatca gatttgaggc ctgctccgca
    34681 catgggtatc aggcagtcag atatccgccc gtttaattca caatacttca gataggctgc
    34741 ataagtggct gctgttgtat gctcacagta aatacccttt gcagcaaggg ctgctctggc
    34801 ttcctgaatc cgattttccg gagccgtgat tatatcaatt ttatacttgt atgtatattc
    34861 caggatttcc ttaccccgca tcggcacgcc gacggcaatt ccctccgcca ttgtgggctg
    34921 tgggattacc tttaccgggt gcttttctcc ggttataact gcctttgcca agggatcgca
    34981 gtgctcactt tgcactgcta ttatcttcgg cattttgtca atgacggcac tctccaggag
    35041 ttcttccaat gcctttacaa tgccgatgaa cagcgtaccg ttgccgaggg gtacaaaaag
    35101 attctcgggg attctttcca tctgctcata gacttcataa atatatgttt ttgtaccttc
    35161 atagaaaaaa ggattataca catggtttgc gtaatacttt ccttctgtct ccaccttcgc
    35221 tctgcataca tccgcacaat tgtcccttgt gccgggaacg acatttactt ttgcattgtg
    35281 ggatttaatc atattaatct tccggggcga tgttccttcg ggtacaaaaa tctcgcagcc
    35341 gatacctgct tttgcacaat atgccgccac actgtttccg gcattgccgc tgctgtcctg
    35401 cacaacggaa tccacaccga ttgccttgca gtgggcaatc agtaccgcag cgcccctatc
    35461 cttgaaagac aaagtcggca tgaaataatc catcttcaga agtacatctt cattaaaccg
    35521 aataacaggt gttatgcctt cgcccaggct gatttcccgc cagtgcgccc cttccatcgg
    35581 cataaaagca cgataccgga aaatgctcca ggtatcatga tccaccaaag acatatcaaa
    35641 cttcggaggt ttgtaatcca atttccacan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35761 nnnnnnnnnt catgatccac caaagacata tcaaacttcg gaggtttgta atccaatttc
    35821 cacaagcctc cgcagtcaca cttcgctttt tttgttgtaa cctcttccgt cgcagcacat
    35881 tttgagcaaa tatatctcat ttccggttca ccttcttcat attttcatcc tttctgagtg
    35941 ccggtttcaa ggctctatta ctccagctac aaagcctgac gataaatacc ctgctatgcc
    36001 aatagtagca tatcagggca tcatgtgtcg acacggtaat ttaacctgct gcaaataacc
    36061 aaataccgaa tattttgctg ttccaatcgg aaaaatattt tttagtattt aaaagttgtg
    36121 atatgcgttc ttaattaatg tgataacttc aaataactat aaacagagga gaaattttat
    36181 ggaaaacagg tatgtgaatt ttaatgaaaa gcggatgtac atattcagcg agcttgtaaa
    36241 gagatactgg aacggaggct tgaagaccgc ccatgatctc accgcactgg ccgattatat
    36301 taagcggaaa tacggcttca gcgattatga attgccattt ataaaggatc atataagagt
    36361 ggcgatggga ttggatccta aaggagacgg cacattcact gatgaactgg acataattaa
    36421 aagtttcagg gaaattgacc ggcctgtggt tgccaggatt gagagcccct gtgaacattg
    36481 cggaaagtta gattgtgaat gcggcaaatg caaacataca gaagcaggca agtacgaaac
    36541 tcatatgtat agcagaaaaa gtgaaccgat aatatccaat gataagtgtc taaactgcgg
    36601 atacagtgct cctgattgtg atttcggggc attggctgac aagattgaat tcataccggt
    36661 tattgatttg ctgaaagacg aaaaaacacc tgtcttcgct tgtgcagcac cggcgatagt
    36721 cgggcagttc ggagaagacg tcactatggg tatgctgcgg gctgccttga agatgattgg
    36781 cttcaccgat atgattgaag tagccatgtt tgccgatata cttaccataa aggaagcctt
    36841 tgagtttaac caacttgtaa aaagtgaaga ggattttttc ctgacaagct gctgctgccc
    36901 ggtatggttc aacatgatta aaactaatta tcccgacatc tataaacacc tgtcaccctc
    36961 cgtgtcacca atgatcgctt cgggcagaat tctgaaaaaa ctatataatg atgccaaggt
    37021 tgtatttatt gcaccctgta ttgccaaaaa ggccgagatt aaagagccgg acttaaaagg
    37081 agccatagat tatgtcttga atttcaggga actgaaagaa atattcaatg cgctgaatat
    37141 taatccggga gagcttccgg cagatgaaaa agatcaggca tccttcggag gcagagtata
    37201 tgccagaacc ggaggcgtaa gcttttccgt caaaacggtg gtcaataggc ttgaaccaac
    37261 aagagtcatt aagcttaaag caaaaaaggt tgacggagtg aaagaatgca ggagagtact
    37321 ggatgatctg cgtgacggca gaaaaatcaa tgccaacttt attgagggta tgggctgtgc
    37381 cggcggatgt gtaggcggcc ccagaaccaa catcgatgtg gataaagcaa ctaagctggt
    37441 taacgaattt ggagaagatt catttatttt gactcccttt gataatttaa atgtaatgaa
    37501 aatcttaaag caattgggta tcaattctat tgaagaaatt atgcaaaaca aagaaataaa
    37561 taaaatatta tccagggaat aagcgatttg aagattctgc cattatactc ctccctgcaa
    37621 aaataccctg ccatgccgat agtagcataa cagggtatca tattcaatta tacagcctcc
    37681 tactccacag tcacgctctt tgccaggttc ctgggtttat ccacatcgca gcctttttcc
    37741 accgccatgt agtaagctaa gagctgcagg ggcattactg ccactaccgg agccagtata
    37801 tcggagactt ttgggatgta aagtgtgtag tccgccacct tctcaacctc tgtgctgcct
    37861 tcctttgcta aggcgaatac aaaggccctt cgcgccttta cttcttttac gttgctcacc
    37921 atcttctcgt acagctcatc ctgggtgagg actgccaaaa ctatcgtgcc cttttctata
    37981 agggctatgg tgccgtgctt cagctctccg gctgcataag cctcagagtg tatataggat
    38041 atctccttga gcttcaggga gccttcaagg gctacggcat aatctattcc cctgcccagg
    38101 tagaatatgt ccctggcatt ggaattatga gtcgcgaatt tctggatggc tgatttatat
    38161 ttgagagttt cctcaacaag tccgggaagc cttctcattt catcctttat tgattcaatt
    38221 tcctcatcct tcagggtacc cttcagctta gccatgtaca gtgcaattat atagattgct
    38281 ataagctggg ttgtgtatgc ttttgtagat gcaaccgcaa tctcgggccc tgcccacgta
    38341 tataacacgt catgggattc ccgggctatt gagcttccta ccacattgac tatgctgagt
    38401 atcctggagc cttttctcct ggcctccttc aaagctgcca gagtgtccag ggtttctccc
    38461 gattggctca ctattatcgt cagggttttt tcatccacta tgggatccct gtatctgaat
    38521 tccgaggcta tttccacttc cacaggaatt cttgccagct tttctattgc gtatttgccc
    38581 accatgcatg catgataagc tgttccgcag gcaatcatga aaattttatt aaaattctgc
    38641 agttcttcct tcgtaagctt tatgtcatcc agctttattc cggcggaatc cggcagtact
    38701 cttgaggtca tggtatcctt cacggcttta ggctgctcat gtatctcctt tatcataaag
    38761 tgctcaaagc cgcctttttc agctgcggca acatcccaat tcacttcgaa tacttccttg
    38821 ccgacctccc tgccgctttc atcaaagaag cttatcctgt ccttttcaat tactgcaagc
    38881 tcgttttcat taagaaggta tatccttctt gtatggttca gtattgcagg tatgtctgag
    38941 gcaatgaaat tttcaccctt ccctattccc actatcaggg gattttcctt ccttacgcat
    39001 attattttat caggctccct tctgcttatc acggcaaaag cataggaccc cctgattttt
    39061 tcaagaacct tcgttactgc ctcaagcagg tcatctttat agaagtaatc cacatagtgc
    39121 gccagcacct ctgtgtcagt ctcagacctg aatgtatacc ccttctcaat aagcatgctc
    39181 ttaatgctca tgtaattctc aattataccg ttgtgaacta ctgcaatatc accggaacaa
    39241 ttggtatggg gatgcgaatt cacatcggag ggttcgccgt gggttgccca tcttgtatgc
    39301 cctatcccca ggcatccgtc caaggtctcc ttcttcagat gctcttcaag tacggagagc
    39361 ctgcctttat atttccttat ctctatgttt tcctttgtat ataccgccac tccggcagaa
    39421 tcatagcccc tgtattcaag cttgcgcagc ccttccatca agatgggcac tgcgttttta
    39481 tctcctatat aaccgacgat gccgcacata tataaaatcc cctctcaaat attgtaagtg
    39541 gataagtatg gtgcctggca ctcaattcgt aagcggataa gtatggtgcc tggcgcctaa
    39601 ttcaactttt cctctatgag ctt
//
LOCUS       DAOOAB010000021        38931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1346_length_38931_cov_15.409584, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000021 DAOOAB010000000
VERSION     DAOOAB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 38931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38931
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_38931_cov_15.409584"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1230
                     /locus_tag="PKU88_06370"
     CDS             157..1230
                     /locus_tag="PKU88_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HPX68946.1"
                     /translation="MWNKRNKKILKRLKLTILIPLGMLLVYFASRNPELVERLYSNGI
                     YKPLGSILSKAFGILPFSAAEFIVILVPAVFIVYTVRALINMQLIKYLTNILVIVSIV
                     FFSFIGVWGLNYYRMPFASIAGLEVRPASVYELESLCLDLIERANALRLSVSVDSEGN
                     VDIPGSPRDILKNCHKGYESVAGKYPELSGSYSHPKPVLLSRLMNYTGLCGVYFPFTG
                     EANVNIAIPESTLPYTASHEMAHQRGFSREDEANYISYLACSAHPDVNYRYSGILLAL
                     TNSMNALHDTDQALFKKLSESYSDEVRHDLASINEFWAGYEGPVERATDRINDKYLKA
                     NNQKDGVKSYGRMVDLLIAEHRK"
     gene            complement(1387..1638)
                     /locus_tag="PKU88_06375"
     CDS             complement(1387..1638)
                     /locus_tag="PKU88_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68947.1"
                     /translation="MEINDMDMHQLEDVLYETSKFNDNDFSTFEEALLDMYGGINTQN
                     LKRIIDFVEHELGIRSDSDLTHLDRNQKKKIVEFASTLE"
     gene            complement(1828..2265)
                     /locus_tag="PKU88_06380"
     CDS             complement(1828..2265)
                     /locus_tag="PKU88_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPX68948.1"
                     /translation="MGAEVECIQKMLEDHGIAHTRQREEILSLFIMESKHYKPDEIFK
                     LLRGKGIGIATVYRTLEMLRECGIIKEISVGKERFYELKINESKHIYVHFKCNKCGKF
                     FDYYNVEAVQEYVSAVDQVEKNMNVKVNDVNINLYGICSHCKS"
     gene            2468..2716
                     /locus_tag="PKU88_06385"
     CDS             2468..2716
                     /locus_tag="PKU88_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68949.1"
                     /translation="MANSYGSVNPAVAAAGCLQGKAVSPFADLCHNNGFLLLLLLFIL
                     LAAGPSWGIDNNFFFIIILAIIAFGGFGRTFGFNGAPV"
     misc_binding    2830..3073
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3135..4511
                     /locus_tag="PKU88_06390"
     CDS             3135..4511
                     /locus_tag="PKU88_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPX68950.1"
                     /translation="MSKIPYRVYLSEKEIPRHWYNLRADMKENHDPYLNPGTMKPAEI
                     NDLYPVFCEELAKQEMDSETRYVDIPDEILEFYKIYRPSPLCRAYNLEKALDTPAKIY
                     FKFEGNNTSGSHKLNSAIAQVYYAKKQGIASLTTETGAGQWGTALAEACAYFGMPLTV
                     YMVKVSAEQKPYRKSIMETFGAKVIASPSNLTEAGRSILAKNPDTGGSLGCAISEAIY
                     HAVNTKNCRYVLGSVLNQVLLHQSIIGLETKLAMEKIEEYPDIIIGCAGGGSNLGGLI
                     APFMQDKLTGKANPCFIAVEPASCPSLTRGRYAYDFCDTGKITPLAKMYTLGSGFIPS
                     SNHAGGLRYHGMSPMLSKLYHDGYINEARAEEQTRVFEAAAAFARYETILPAPESSHA
                     IRAVIDEALKCRETGEAKTILFGLTGTGYFDMQAYIQYNSGKMSDYIPTDEDLQKGFS
                     ELPNIPGL"
     gene            complement(4576..6012)
                     /gene="glgA"
                     /locus_tag="PKU88_06395"
     CDS             complement(4576..6012)
                     /gene="glgA"
                     /locus_tag="PKU88_06395"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPX68951.1"
                     /translation="MDSINVLFAASEAVPFAKTGGLADVAGTLPRELVKLGADVRLII
                     PKYKSIPRNFVEAMEYIGYVYVDLAWRHQYCAVLKLEYQGVITYFIDNEYYFNRDCLY
                     GHFDEAEQFAFFSRAVLKVLPLIDFKPDIIHCNDWQTGVVSAFLKAGFGHIGFLKDIK
                     TVYTIHNLKYQGMFPKEVLGDILGLSWDYFNPEGLELHDCVNYMKAGIAYSDIVTTVS
                     KTYAEEIKTDFFGENLNGILTKRSESIFGILNGIDYLENDPETDTRIYKSFNAEDLEG
                     KYENKKMLQESLGLPQREEVPVIGVVSRLVGQKGFDLIAHVLDEMLQMDIQLVVLGTG
                     DSNYENMFRNAAERYPGKVSANIKYDGVLAQRIYAGSDMFLMPSLYEPCGLSQIFSLR
                     YGTVPIVRETGGLKDTVMPYNEFTGEGTGFSFANYNAHEMLDTVKRAISFYKNKYIWE
                     KIVKSGMKQDFSWSKSAKEYMELYEKMV"
     gene            complement(6036..7178)
                     /locus_tag="PKU88_06400"
     CDS             complement(6036..7178)
                     /locus_tag="PKU88_06400"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPX68952.1"
                     /translation="MIKRIVAAPDSFKGSLTAKEAAEAMEAGIKRYDNSIEVVKVPMA
                     DGGEGTVQSMVDATGGHMISVTVKGPMLSEVEAFYGILGDGSTAVIEMAAASGLPLVK
                     PEERNPLIATTYGTGELIRHALDMGCRRIIIGIGGSATNDGGAGAMQALGVRLLDDFG
                     NDIGFGGGSLGSLYRIDINNLDPRIKDTVITVACDVDNPLCGHKGASQVFGPQKGADD
                     AMIEELDRNLAHYADLVESTLGVKIKDYPGAGAAGGLGGGLLAFLSATLKKGVDIVIE
                     TVELEKKIRDADLVITGEGRMDNQTIYGKTPFGVAKAAEKYGIPVIAICGSIGKGAEV
                     LYDNGFASIFSIIDKPMCIDEAMKKGSELVQNCSERVIRALSITEQ"
     gene            complement(7325..7948)
                     /locus_tag="PKU88_06405"
     CDS             complement(7325..7948)
                     /locus_tag="PKU88_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HPX68953.1"
                     /translation="MSFVQNPVRISTRRITAQDNMVTIDYPVVKGLESPAAQHRINMT
                     IAAAVNMLMREQGFYEKQKTEMLGWYEIKTNERGILSLTIGNYAFTYPSAHGLTIVKG
                     LTFDVNSGYSIELKDLFKPEADYDKVLSGIVEQQITDRNIDLLDEFKGIRPYQDFYIA
                     DKSLVLFFQLYEITPYYMGLQYFPISVYQIQDIIAEEGALGIMAAGD"
     gene            complement(7952..8410)
                     /locus_tag="PKU88_06410"
     CDS             complement(7952..8410)
                     /locus_tag="PKU88_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HPX68954.1"
                     /translation="MAIANIKGGPLAERLKGTVLFTNVPGGVWVCVHVSGLSDYKAAK
                     DGGQPVGPHGFHIHENGSCEVGDPEEPFKAAGGHWNPANQPHGNHAGDFPVLFSNKGM
                     ARMCFFTDKFKTRDIIGKAVIIHESPDDYRSQPAGNAGKRLGCGVIREGR"
     gene            complement(8492..9361)
                     /locus_tag="PKU88_06415"
     CDS             complement(8492..9361)
                     /locus_tag="PKU88_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX68955.1"
                     /translation="MSDIAGKGCEILVPFSERKPLPEIERSLIKKYRKHIWTRFMKAV
                     KEFELVKEGDRIAVGVSGGKDSMLMAKLMQELQRHGDASFELEFIAMDPGYHENIREL
                     LIENCNYLEIPIHIFESGIFKIVDKIAKDYPCYMCAKMRRGALYGKAMELGCNKLALG
                     HHFNDVIETTMLNILYTGSFKTMLPKLKSTNFKGLELIRPLYYVDELYIERFTQSSGI
                     WPLNCACMVAAGKIANKRCEIKALIKELKEKIENADTNILRATQNVNLEAILGWHKGG
                     QQYSYLDFYEEQE"
     gene            9543..10532
                     /locus_tag="PKU88_06420"
     CDS             9543..10532
                     /locus_tag="PKU88_06420"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HPX68956.1"
                     /translation="MLKIPERIHLANLPTRIEKLDKLTSVLGGPEIYIKRDDQTGTEL
                     SGNKVRKLEYSVKEALDMGCNYLITCGGVQSNHCRATAAAAAKLRMKSCLVLRGDAGS
                     EAEGNLLIDRLLGADIRFITAEEYSDQRTEIMEAIKRETDDKGFKAYIIPEGASNGIG
                     SFGYYTAMEEIIEQEKELGIHFDCIVLATGSGGTYSGLWLANKLLGHSSDVCGICVGG
                     DAGYFKAIIPEILFESMEYMDVKIPVSPEDINIIDGYVGRGYALSRPEELRFIHGFAE
                     LEGIILDPVYTGKAMYGLTEEIKKGKFKNYKNILFIHTGGLFGLFPQGDLFKF"
     gene            complement(10529..11053)
                     /locus_tag="PKU88_06425"
     CDS             complement(10529..11053)
                     /locus_tag="PKU88_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68957.1"
                     /translation="MSLRVGFDNNLISQNSGYASGASGQAAMSPDGKKSVEQGQTIDS
                     IRNDKLMKKIGAVECQTCSNRKYVDGSDDAGVSFKTPTHVSPENSMAAVASHEQEHVS
                     REGAKARAEGREVVSQSVRVFTDVCPECGRVYASGGKTTTITKADNKNTDYFMDNMRK
                     SMGIHFGKYIDKRI"
     gene            complement(11113..11982)
                     /locus_tag="PKU88_06430"
     CDS             complement(11113..11982)
                     /locus_tag="PKU88_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPX68958.1"
                     /translation="MVRVIVDSSCDLPDELLEEYEIKSVSQRVYINNREYLDKVTINA
                     KEVYEAMKKGIIPQTSLPRPMEIYGHFKQCCQEGNDFIYIALSSKFSGTYQLAVSLMQ
                     ELQGQYDKVRMDVIDSKSGSTAIGLIALQAAKLARAGLGFDMIVRQICELADHVEHIF
                     MVQDLSWLIRGGRISRTEGIIGSILNVKPILHVKDGAAEILEKVRGRKKALDSIVNIM
                     EERIKDFPDQTIGISYAWDQDIAYEMRDMIKQRLEVKNFVINEIGAALASHLGIGGVG
                     VFFFNMEPDFYME"
     gene            12250..>13277
                     /locus_tag="PKU88_06435"
     CDS             12250..>13277
                     /locus_tag="PKU88_06435"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPX68959.1"
                     /translation="MSLNSLKELNIGNLTARLPIIQGGMGVGISLSGLASAVANEGGV
                     GVIAAAGIGMLDYDFSTKFIEANIARLRSEIRKAREKSKGIIGINIMVALSNYAEFVK
                     ASIEEGIDIIFSGAGLPLNLPQYLEEKYETKLVPIVSSARAAGIICKKWLDKYNYLPD
                     AIVVEGPKAGGHLGFKEDQIFDPDYSLEKILPEVVDEVKAFEDKSGRSIPVIAAGGIY
                     SGEDIYNIMKLGASGVQMATRFVTTEECDASMDFKKAYINSNKEDVSIIKSPVGMPGR
                     AIRNSFTEDILKGANKPYKCPYHCIVTCDYKNSPYCIALALTNAQKGKLENGFAFAGV
                     NAYRADKV"
     assembly_gap    13278..13377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13546..13839)
                     /locus_tag="PKU88_06440"
     CDS             complement(13546..13839)
                     /locus_tag="PKU88_06440"
                     /inference="COORDINATES: protein motif:HMM:NF021082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HPX68960.1"
                     /translation="MDKKVVKELNAYLMGEYVAVKSYEKFIERTKDSDVRQKLQNIQQ
                     DHEKHASKISERISSLGGKPVKGAGCMGIMSDMKNLIKRNRDDTEFILEDAGS"
     regulatory      14010..14139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14205..14768
                     /locus_tag="PKU88_06445"
     CDS             14205..14768
                     /locus_tag="PKU88_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPX68961.1"
                     /translation="MENKTNKLVRIAVLSALSLVLVVLVKFPIIPSAPFLEYEPGDVP
                     MLVAAFMYGPVAGLVMTVIVSAIQAFTVSAASGWVGMVMHIIATGTLVVVSGAIYRRF
                     HTFKGAFAALAAGSLGMILVMIPMNLFFTVKFYGVPYDAVVAMLPTAILPFNIIKSLA
                     NSLIVILVYKPLSRFLKGESKIQKKAA"
     gene            complement(14896..15396)
                     /locus_tag="PKU88_06450"
     CDS             complement(14896..15396)
                     /locus_tag="PKU88_06450"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPX68962.1"
                     /translation="MLVLMYMTGNFQNLYQNGKQIVSSAATTPDAATQPEVSQGGLID
                     SAVVRGKTVLNISNMPIGAILEEMGGTYEWNAAEQKATIHWNGMTVELWNGQKSIKVN
                     GVEKEVDIAPNVGTTASVILPSGFAANEPDRILSWEEGSIQIWNSVQKDIQNPSDKKM
                     SRAFKK"
     gene            complement(15408..15611)
                     /locus_tag="PKU88_06455"
                     /pseudo
     CDS             complement(15408..15611)
                     /locus_tag="PKU88_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
     gene            15873..18059
                     /locus_tag="PKU88_06460"
     CDS             15873..18059
                     /locus_tag="PKU88_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HPX68963.1"
                     /translation="MGKTLVLAEKPSVGRDLAKVLNCNQNSNGYLVGQKYIVTWALGH
                     LVTLADPEAYNKKYKNWNLEDLPMLPDRMELVVIKETSKQYGIVRSLMKQQDIDELVI
                     ATDAGREGELVARWIIEKAGFRKPIKRLWISSQTDKAIKEGFNQLKPAKEFDNLYKAA
                     QCRAEADWLIGLNVTRALTCKYNAQLSAGRVQTPTLAMIVQRENEIKSFVPKDYWTIE
                     AKVNGFTLLWKDKSGNARLFDKSKAEAIVSKLTGQTVEITDIKKEAKSEPHPLAYDLT
                     ELQRDANKRYGYSAKQTLSIMQSLYERHKVLTYPRTDSRHITADIVPTLPDRLKSIAI
                     GPYAAIAQNLLRGKISTTSRLVDNSKVSDHHAIIPTEQPVRLSALSNEERNIFDLVAK
                     RFLAVLSPAYQYEQTTVKAESSGEQLIARGKVTKSLGWKAVYNDRSNTDSANDDEDKE
                     QSLPPMQKGDKLKLLSVKSVNGKTKPPARYTEATLLTAMEHPGKFIEDESLREALDRT
                     NGLGTPATRADIIEKLFNTFYIERKGKEIYPTAKGIQLIDLAPSELRSAELTAKWEQQ
                     LYDISKGKANADVFISNIRSHAGKLVSSVAASAEAYRHDNMTRTRCPECGKFLLEVNG
                     KKGRMLICPDRECGYRKGVAQLSNARCPECHKRMELKGEGEGRLFTCSCGFREKLSSF
                     SKRMDEKTESSDKRTVQKYLQQQKKEEPINSAMAEALVKWKEMHDK"
     gene            complement(18099..21704)
                     /locus_tag="PKU88_06465"
     CDS             complement(18099..21704)
                     /locus_tag="PKU88_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPX68964.1"
                     /translation="MAPVRVNINNRELTADEGSTILRIALDNGIGIPNLCYDENLKTY
                     GACGMCVVEVEGNPKLVRACSTPAVNGMIIKTDTPRTIAARKTALTLLSSDHKGDCRP
                     PCVNACPGHTDCQGYVGLVANRQYDEAIKLVKEVIPLPASIGRVCPHPCETACRRTNV
                     EEPIAIADIKRFIGDIDIYNGTYVPEIKEPTNKSVAVIGSGPAGISCAYFLAAKGHNV
                     VLYEDKPYPGGMLRYGIPQYRLPKELLDAEINTLVKMGVDIKCNVKLGEDITLAYLKK
                     TYDAVFIAIGAWASMNMGCIGEDMEGVMGGIEFLRKATLAEPIYLGQSVLVIGGGNTA
                     MDVARTAVRLGAEHVQVLYRRTRAEMPAEDIEIEEAEEEGVEFTYLVAPIEIIEGNGR
                     VKAVRCQRMKLGAPDKSGRRSPEPIPGDEVTIPADLVISAIGQRVNAESVKELELTKK
                     GAIAANANTFETNIPGVFAGGEAVTGPKIAIEAIAQGKNAASVIDSYLKGEIKPVANP
                     CYIEQEDLGPEDFADKEKKEREHQKVYPAKIRKMSFGAISETFDEETAVKEASRCLEC
                     GCHDYFECKLVNYIGKYDIDTKAIKGEIHRKEEADTHPFIKRNAEKCILCGLCFRACE
                     EVIGVTALGLLDRGFDSVVVPEFGLPLEESACISCGHCIDVCPTGACMEKQAINKQVP
                     VELEDTASVCSYCGVGCNVVYQSRGSLVFKSRPNKAKGEDTLCAKGRFGIHHINDGNR
                     LLKPIIRRSGDTVEAEFDDAIALAAKKLQLIKGQYGKNSVAVFMSPKFTNEEAYLTKK
                     ISEKLDTGIIGTLSAKEESGIKGILGCNASANSYDELYNTDLIITVGDVAENHPVLGI
                     KIKDAAKRSKLVSISNSDTRLAEHADVALKIENNIKFLKSFVKSLIDQKFADKDKADK
                     ISIGYDELAKYVDNAPNDEDASKAAKMYGEAKKAIIVIDEETVSEEAIKLIADAAVIT
                     EKIGKPHRGIIIARSKANTQGIIDMGFTVPANKLIESIEKGEIKGAVIIGEDPAASDE
                     RIKELLEKLEFTVCLDLYITETAKLADVVIPVCSAAESEGTYTRSDRKVQRLEAAVKP
                     LTGKTTFDVLSRLGAQMGVMIDTVKEAEEKISNEVKQYSGLFAALTEGIEPYVPCSRG
                     NVYGSQVLYTEGFMTEDRKANLSIPAGEQMFKERTVCDTVQLRFEEYIKEKEIKA"
     gene            complement(21721..23478)
                     /locus_tag="PKU88_06470"
     CDS             complement(21721..23478)
                     /locus_tag="PKU88_06470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPX68965.1"
                     /translation="MKLRIGLGSCGIAAGGKKVREALLNCLKEKDMNIEITSTGCIGM
                     CFYEPIVDVIDDEGEVYTYVNVTPEKAEEIVEQHIKNGVPVEKYIQSTGKKPFKQLEK
                     QVRVVLQNCGIINPEKIEDYLEKGGYKALEKCLKEMTPDQVIEEIKKSGLRGRGGAGF
                     PTWFKWDAARKAKGNEKYVVCNADEGDPGAFMDRSVLEGDPHGLLEGMIIAGYAIGAN
                     HGFIYCRAEYPLAIERLEGAIAQAHEANFLGGNIMGSGFSFDIKIKKGAGAFVCGEET
                     ALIASLEGERGMPRLKPPFPAQSGYFGKPTNINNVETYANVPWIIRNGGAAFAAYGTE
                     KSNGTKVFALAGKIKNGGLVEVPMGMPIREVIYEIGGGIKNDRDFKAVQMGGPSGGCI
                     PKSLIDTPVDYESITRTGAIMGSGGMVVMDEATCMVDMARFFLNFTCDESCGKCIYCR
                     IGTKRMLEILERITKGEGRMEDMDELEQLCISIKDGSLCGLGQTAPNPVLTTLKYFKD
                     EYIAHIRDKKCPAKQCKSLITYSILKDKCTGCTACARKCPTEAITGERKQPHSIEQEK
                     CIKCGNCAITCKFGAINVE"
     gene            complement(23475..24005)
                     /gene="nuoE"
                     /locus_tag="PKU88_06475"
     CDS             complement(23475..24005)
                     /gene="nuoE"
                     /locus_tag="PKU88_06475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPX68966.1"
                     /translation="MSCTCEKKLETNFCKKEVDLSLMEPVFKKYEGQSASLISILQDT
                     QGIYGYLPLEALKTIADRTGNKKAKIYGIATFYSQFRLNPIGKHIILQCQGTACHVNG
                     SDKISEAICDELGIEPGGTSKDGMFTLENVACLGCCSLAPVMMINGEAYGNLTPNSAR
                     EVIRGIYKKEKGGASN"
     gene            complement(24097..25011)
                     /locus_tag="PKU88_06480"
     CDS             complement(24097..25011)
                     /locus_tag="PKU88_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX68967.1"
                     /translation="MSNKTLGIIYAVSSAVSFGFVPVFAVIAYNAGTNAVTVLLLRFL
                     IASLILAGILRCRKVELKVGKKLFCRLAYIGIIGYAATTITVFLSYNYISIGLAMTLH
                     FIYPAVVAVLSFFIFKEKMPALKMIALAISIVGVYILAGNTGSSVDLKGVALALASGI
                     LYSIYTIEIGKEELMAMDGLLITFYVSFFSAAAFFIFGAASRNLMFSMQLPAVLGILG
                     LALISTVFGILAYNKSIQMIGPTYAAIFSTSAPITSVVMGIILFSEKLSLTSAIGSIM
                     VIASVLLFSCDKNAKTKDDKVLDTSHSI"
     gene            complement(25164..25373)
                     /locus_tag="PKU88_06485"
     CDS             complement(25164..25373)
                     /locus_tag="PKU88_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HPX68968.1"
                     /translation="MDRLALILVIVGALNWLLVGLFQYDLVASLFGGQDELLSRLIYT
                     LVGVAGIWSISLLFRERERAAGNEK"
     gene            25627..26172
                     /locus_tag="PKU88_06490"
     CDS             25627..26172
                     /locus_tag="PKU88_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68969.1"
                     /translation="MKNIKYIQIIKGFIALIITVALLYTGQALWRSYTIDLPLDKTLN
                     EIDGVEEVSWDAGSSFNDAVIINIKLGNIDNLQKTYKEIKEKIDLTLNGKKYSLEIAD
                     DRSQELEQAYYDIHYYVQKSIIDGDFPSLEAEVREKAEMIGAEAKVYVDEQNIYLQFT
                     KKDNSLFAAIGRHFDRTGGNL"
     gene            26172..27653
                     /locus_tag="PKU88_06495"
     CDS             26172..27653
                     /locus_tag="PKU88_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX68970.1"
                     /translation="MKNREIFAGAAAGALIFLALQRVNILPVVFILGLIGIVYYVNFG
                     RLSGNSISFASAERNICSVSFDDIGGQEVAKNELREALEFMKNDEQIRLLGIRPLKGI
                     LLSGPPGTGKTLLAKAAARFTDSVFLSASGSEFVEMYVGVGAQRVRSLFEQARKAAEY
                     LKKSSAVIFIDEIEVLGGARGQNNGHQEHDQTLNELLVQMDGISSDDRIKILVIGATN
                     RIDMLDPALLRPGRFDRLVKVDLPDKKGRMHILELHVRNKPLASDVNVEALAKDTFGF
                     SGAQLESVANEAAILAMRDGSDKIDSNHFRSAVEKVMMGEKLDRLPSNEEKRRIAVHE
                     SGHALVGELRLPGSVASVNVASRSNALGYVRQTQENDMYLYTCDYLKSRISAALAGII
                     AEELIFGNRSTGGSGDFKHASDIAKQIVYSGMSELGIISSEDIPGDQLHSAITSILRD
                     VESSTRDFLSNYRRELKRISELLLDKETISGEELRLILISECA"
     gene            complement(27692..28543)
                     /locus_tag="PKU88_06500"
     CDS             complement(27692..28543)
                     /locus_tag="PKU88_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPX68971.1"
                     /translation="MNFLVDYHVHSIYSSDGHNTIMEMCESAVNNGIGEIAITDHFEP
                     NIADEYGSSYNQKGYWAHINKARQAFKGRLNIKLGVELGQPHLFSEASSAVIADFPYD
                     YIIASVHKLPAGMDIKELDYSRIGEEELCEMYLRQLKQLVYWNNFDCIGHLDLIKRYS
                     APVYKKNISLTCRHELLKGVLLLAVQSGKGIEINTSGLRQSPKEAFPGLDVVKLYRNM
                     GGEILTIGSDAHMAEDVGRGLKEALDMAREAGFRHITVYNRRKPEWVSITEGKNTFGS
                     AKQIINR"
     gene            complement(28697..30364)
                     /locus_tag="PKU88_06505"
     CDS             complement(28697..30364)
                     /locus_tag="PKU88_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPX68972.1"
                     /translation="MPDLNSLIQESITFQIIFNLFGGTALLMFGVDFMGGGLEKASGK
                     VMKRILSVLTKNIYVAFFVGTILTMLVQSSTAITVMTVGFVNAGLMNLTQAVGIIYGT
                     NIGTTITAQLMSLKLTYIALPIIAIGFYIFSFSKKDSTRSLGQAIMGFGLMFLGLKTL
                     NASLPYIKDSETAMQLFLQYGQNPFLGLFVGMIATMLIHSSAATVALTISLFSADLIS
                     FYAAVGLTLGDNIGTCVTAQLASLKANTAARRTAWAHTLYNVIGVVIAIIVLRPFSGM
                     IEWITFSILHEPKAFLVANTHTFFNIISALVFLPITKYYVLFLEKIIPDRRDKKYEAG
                     TLDRLLLDTPVAALKAATQEVINAAEVAKNMLNDCLISLIEDNESKIDSVLQNEEKLN
                     IMQTEITQYLVEISHKDLDSYQSARIPALINTINNFERVGDHCEDITELSQSKINRKI
                     IFSEKGIDELETLYQEVMSMMNECCLAYSKDDAELALQAAAREEVVDELTLKFKEKHI
                     RRLEEGTCSVQAGVIYLDAITHLERIADHLHNVCLILSEGRRNADKN"
     gene            complement(30593..31309)
                     /locus_tag="PKU88_06510"
     CDS             complement(30593..31309)
                     /locus_tag="PKU88_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPX68973.1"
                     /translation="MSRNIRVFFAGFLCCILLTAGMMAVIGEPADTEVKAILSNTIKM
                     KLFGNDFAPKNDDGTYLKPLIYNGRTYLPVKPLGDALGIAVEWDGVNKIVWLGGKTQI
                     VPINDTSMYEDYSRTIVTTDTDKLTTADTTYKWGIANISPLNSVYYGCCLKPEGKYKR
                     FTASIFLSEDTKKELTMDIRKDNKQGVVLKSLQLKPGETVEIDIDISGVDTLFIFSQV
                     GSTQKLIIGEPAFRNDTTPQ"
     gene            complement(31442..32278)
                     /locus_tag="PKU88_06515"
     CDS             complement(31442..32278)
                     /locus_tag="PKU88_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HPX68974.1"
                     /translation="MAEALVNKILPFSSVDGPGNRTVIFFQGCNFNCLYCHNPETINR
                     CTGCGLCLDVCPAGALRSRNGRIEWNSANCTGCDACLAACPGKSSPKAVSMSVNDIMK
                     TVEKVKPFISGITVSGGECTMQKEFLAELFDAVHKLGLTSYVDTNGCIDFSKEPELTF
                     AMDKAMLDIKSYDDGEHRMLTGMSNALVLKNAEFLANKQKLYEIRTVIVPGILDNYRN
                     VAEVSKLIAKLDADIRYKLIRFRPVGVAGDIKKVGIPSDELMEELKKTAEKNGCRYVV
                     VV"
     gene            complement(32271..33764)
                     /locus_tag="PKU88_06520"
     CDS             complement(32271..33764)
                     /locus_tag="PKU88_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HPX68975.1"
                     /translation="MSEVLSIIKDRTLTYEQKIVSLARAAENTLSVLPISKEIQEYRR
                     EGIICDLNEGNAPYRPRYIVPDYERFMRQGSEFLRLKPARDIWEATNNLLILYSHVPS
                     VTTMPVYVGNLDTLLEPFIKDEEEAYKAVRLFLMHLDRTVTDSFCHANIGPGATKAGR
                     LILRAEKELQDAVPNLTIKYSKDTPEEFALEAVETALVSAKPSFANHEMFVNDFGINY
                     SIASCYNGLPIGGGSYTLVRLVLARLAKQSNNAGHFLEEVLPEAVSKMREFMDQRIRF
                     IVEESGFFESSFLADESLIYKDRFTAMFGMVGLAECVNHLLGAKEQKDRFGHNDAANA
                     LGQRIIERMHQLVSEHNNQYCKATDGRFLLHAQVGIDTDYGISPGCRIPIGEEPEIYE
                     HLLQSAPFHKYFPSGIGDVFSFDETVKRNPAYLLDIIKGGFSEGLRYISFYASDSDLI
                     RITGYLVKKSEMEALQQGKQALHDTVALGLGAVQNSGILERKVRHNG"
     gene            complement(34163..35461)
                     /locus_tag="PKU88_06525"
     CDS             complement(34163..35461)
                     /locus_tag="PKU88_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase"
                     /protein_id="HPX68976.1"
                     /translation="MYKNPYMCPMNPFYICPFQNLTEDTEYDDSFIEVYYDELYFEDE
                     YDEYGYEGMRQRPQDVSRIMPLINRRFAGHYQELERYGVRRSDTRNIFRNTVIYVLRN
                     ANNYNGTIEQRTNALLNALRRDNPYAFTILRRKGVPNARINQIMRDIIQFTLENIEVA
                     EGTGWSQWEDLGGILTSAPAAASWQPNRLDVFARGQNQALWHVYWNGARWSAWEDLGG
                     ILTTGPGEVSWGTNRIDVFARGQNQALWHIYWDGIRWSAWEDLGGIITSEPAAASWQA
                     NRLDVFARGQNQSLWHKYWDGIRWSEWEDLGGVLTSAPAAVSWGPNRIDVFGRGQNQS
                     LWHKYWDGTRWSAWEDLGGGQITSGPAAASTGSNRLEVFARGQRNELMIRTWNGTRWS
                     GWSPLGGVLTSDPAAVSWGGNRLDVFARGQNNHLWHIWRA"
     gene            complement(35622..36368)
                     /locus_tag="PKU88_06530"
     CDS             complement(35622..36368)
                     /locus_tag="PKU88_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68977.1"
                     /translation="MTILKKGQLTRHINESKEQDEYVMRIITPEELGDAVALQKYVYE
                     QLPNKQVLYMDSYEEMYDDMKQGAKIIGVFNKTGDIIAYRYIGFPGCSEKNLGNDINM
                     PRQELAKVAHLETTVVHPEYRGNSLQSLTLQQAIPMIKDFGYSHLLCTVSPQNIYSLY
                     NIIKNGLRIKALKKKYGSEKDGKDGMWRFILHRNLEPRAVRKVSQNFGIPIGDFELQR
                     KLIDEGFVGLWLYKESKLLNYVRFEDAIAN"
     gene            complement(36580..37218)
                     /locus_tag="PKU88_06535"
     CDS             complement(36580..37218)
                     /locus_tag="PKU88_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPX68978.1"
                     /translation="MNLKSHGEDKIPAAKYEKIALDIAYSILNEEWKAGEMVKGRSTL
                     SGKYGVSPETIRRALKLLEELQVVNVIEKKGILIKSKDAANNFIKEHQSKDRILSMRE
                     EIVKLMEEKQALEKQVLERLDSVIEQAMLLRNIGIIFPLELKIPEKSHLVGKTIGEVR
                     FWNHTGATIIGINRSGHLYLSPGPKMVFFPNDIILYVGSDAEISEKVAKYVK"
     gene            complement(37320..38690)
                     /locus_tag="PKU88_06540"
     CDS             complement(37320..38690)
                     /locus_tag="PKU88_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX68979.1"
                     /translation="MDTSKQLGEEKISKLLLKFSIPAIVGMLVNALYNVVDRIFIGNS
                     VGPLGIAGITIGFPIMLVIMAFAMLIGIGANSLISIRLGEQRKEEAELILGNAVVMLV
                     ITSMTISGTGLIFIEPLLRIFGASAAVLPYAKEYLSIILLGTVIQTVGFGMNNFIRAE
                     GNPRIAMFTMIIGAILNTILDPIFIYVFGLGIRGAAIATVLSQAVSASWVLYHFFGGR
                     STLKVHTKNLRLHTPIVVKIVSLGAATFFMQMAASVLNAIMNKSLVIYGGDIAVSGMG
                     VIMSIAMLILMPIFGINQGAQPIIGYNYGALEFDRVKKALKLAVAAATIITTIGFILT
                     RVFPSQLILLFNRTDKDLLNFGTRAISIYLIFLPIIGFQIVSANYFQAVGKPKHAAFL
                     SLSRQVLILIPALLILPLFFGLEGLLMAGPASDLISSVITGIFLIRELKNLDARHRGA
                     VVQEQN"
BASE COUNT        10442 a         9315 c         7524 g        11550 t
ORIGIN      
        1 tgtcccactg gaagtgggac aggggaaacg tccccgtgtc cctgtccccg tgtacccgtc
       61 gtattattca tataatttgt aattaaacct gtgtacaaaa tgttataata tatacggtta
      121 tatgtgcaat taaatgaagt aaaggctgat ttctatatgt ggaataaaag gaacaaaaaa
      181 atattaaaaa ggcttaagct tactattctt ataccccttg gaatgctcct tgtctatttc
      241 gcttcaagga atccggaact tgttgaaagg ctgtattcca atggaattta taagcccttg
      301 ggaagcatcc tttcaaaagc tttcggaata ctgcccttct ctgctgccga gttcatagta
      361 atacttgttc ctgccgtctt tattgtttat actgtaaggg cccttataaa catgcagctt
      421 ataaaatacc tgaccaatat tcttgtaata gtcagtatag tctttttcag cttcataggc
      481 gtttggggat tgaattatta cagaatgcct tttgcaagta ttgcaggcct ggaggtacgt
      541 ccggcatcgg tctatgaact ggaaagcctg tgcctggatt tgattgaaag ggcaaatgcc
      601 ctaagattgt cagtaagtgt ggattcggaa ggaaatgtgg atattcccgg cagcccccgt
      661 gatatactga aaaactgcca taagggttat gaatcggtcg ccggcaagta tccggagtta
      721 agcggcagtt acagccaccc caaacctgtt ctgttatccc gtcttatgaa ttatacgggc
      781 ctctgcggcg tgtacttccc ttttaccgga gaagctaatg tgaatattgc aatacctgag
      841 tcaacactgc cctataccgc atcccatgaa atggcccatc aaaggggatt ttccagagag
      901 gacgaggcca attatatttc ctatctggcc tgtagtgccc accctgatgt caactacagg
      961 tattccggca tattgcttgc attgacaaac tccatgaatg ccctccatga tactgatcaa
     1021 gcattattca aaaaactctc tgaaagctac agtgatgaag tccgtcacga ccttgcgtcg
     1081 ataaatgaat tctgggccgg atatgaaggc ccggtagagc gggcaaccga cagaatcaat
     1141 gacaaatatc ttaaagcaaa taatcaaaag gatggggtca agagctacgg cagaatggta
     1201 gatttgctta ttgcggaaca taggaaataa aaaacgtgtc tcacgacgcc tttttttcgc
     1261 tagggaaccc tatggagcac cctccttcat cggcaggggg cttctccccc ttgcatcccc
     1321 cattgtatag gaaagtatac tttcccatac aataataaat agaggagaat gatgttttat
     1381 tctcccctat tccagcgttg aagcaaattc aactattttt ttcttctgat ttctgtccag
     1441 atgcgtcaaa tcagaatcag atcttatccc aagctcatgc tcgacaaaat caatgattct
     1501 tttcaggttc tgagtgttta tacccccata catgtccagc aatgcttcct caaaggtact
     1561 aaaatcattg tcattgaatt ttgatgtctc gtacagaaca tcctccagct ggtgcatatc
     1621 catatcgttg atttccattc ccacggcatt cacctatcct tgcatagttt taaatacttc
     1681 atacccaaat tattttgcct ttttattaag aaactatgtg ccgcagcgct ttatgggatt
     1741 gctattttcc atatcctcaa gagtttgaaa aagtcaaaaa aaaacagccg gagaaacaaa
     1801 ttcattcctc cggctgcgta tagactttta agacttgcag tggctgcata ttccataaag
     1861 gttaatattc acatcattga ctttcacatt catattcttt tctacctgat caaccgcgga
     1921 aacatattcc tgcactgctt caacattata gtagtcaaaa aactttccgc acttgttaca
     1981 cttaaaatgc acgtagatat gcttgctttc attaatctta agctcgtaga acctttcttt
     2041 gcctacactg atttctttta ttataccgca ttcccttagc atctccagtg tcctgtatac
     2101 cgtagctatg cctatcccct tgcctctgag aagcttgaaa atttcatcag gcttgtaatg
     2161 ctttgattcc ataataaaca agcttaatat ttcttctctc tgacgagtat gcgctatacc
     2221 gtgatcctcc agcatttttt gtatacattc aacttctgca cccatagtat cacccaattt
     2281 ctatgaaaat caactttgtt caataattca caatttaatt ttacatcttc gttaaagaaa
     2341 tatcaagttt aattgagatt ttctatgctt tttttcatga tatgtatagt gtcaattttt
     2401 atgcatgtac atagtatgtt attgaagaaa gggtaaacaa cctgtatttc tgaaaggagg
     2461 tggaattatg gctaatagtt atggatcagt caatcctgca gtagcagctg ccggctgctt
     2521 acaaggcaaa gcagtatcgc cttttgcaga tctttgccat aacaacgggt tcctgctttt
     2581 gcttcttttg ttcattcttc ttgcggcagg tccatcttgg ggtatagaca acaatttctt
     2641 cttcatcatc attcttgcga ttatcgcatt tggtggtttt ggaagaacct tcggtttcaa
     2701 cggcgcaccg gtatagtcag ccggtcttct aaccgctata agcccacctg tctccctggt
     2761 gggcttaaaa gttttttcaa ttttcttctt gacaaattga aattatagag ctaatattga
     2821 tttcaatatc aaaaactgat gattaagaat agtaagctta gcttaatgct cacagagagc
     2881 cgcagttggt gtgatgcggc agcaggtgct ttgctgaatg gacttatgaa ggcagtacga
     2941 aagatttatc gagtagttac tgacgggaac tctgcccgtt ataaatggag cgtatattat
     3001 tgtatacgaa ttgagtgggt gatttttcac caatacgggt ggtaccgcag gagtatatgc
     3061 tcttgtccct atttttaggg gcaagagctt ttttatatac ttcaataatc aatattaaga
     3121 aaggagaagc aaaaatgagt aaaattccct acagagtata tttgtccgaa aaggagattc
     3181 ccaggcattg gtataacctt cgggctgata tgaaagaaaa tcatgatcca tatctaaatc
     3241 caggtacaat gaagcctgca gaaattaatg acctatatcc cgtattctgc gaagagcttg
     3301 cgaagcagga aatggatagt gaaaccagat atgtggatat tccggatgag attttggaat
     3361 tctacaaaat ctatcgccct tctcctcttt gccgtgcata taacttggaa aaggctcttg
     3421 atactccggc aaaaatttat ttcaaattcg agggcaacaa tacttcaggc agccataagc
     3481 ttaattctgc aatagcacag gtatactacg caaaaaaaca aggcattgcc tccctcacca
     3541 cagaaaccgg tgccggccag tggggtacgg cattggctga agcttgtgca tattttggta
     3601 tgcctctgac tgtatatatg gttaaagtat ctgcggagca aaaaccttac cgtaaatcaa
     3661 tcatggaaac ctttggtgca aaggtgattg caagcccgtc caatctgaca gaagccggac
     3721 gctctattct tgccaaaaac cctgatacgg gcggaagtct tggctgtgct atatccgaag
     3781 ctatttatca tgcggtaaat acgaaaaatt gccgttacgt tctgggttcc gtactcaatc
     3841 aggttctgct tcatcaatcc ataatcggtt tggaaacaaa acttgctatg gaaaaaatcg
     3901 aagaatatcc tgatatcata atcggctgcg caggaggagg ttctaacctt ggcggcctaa
     3961 ttgccccctt tatgcaggat aagcttacag gcaaggcaaa cccctgtttt attgcagtag
     4021 agcccgcctc ctgcccgtcc ttaaccagag gcaggtatgc atacgacttc tgcgatacag
     4081 gcaagatcac tccccttgca aaaatgtata cattaggctc tggattcata ccttcctcaa
     4141 atcacgcagg aggtttgcga tatcatggta tgagccctat gctttcaaag ctctatcatg
     4201 acggctatat aaatgaagcc agggctgaag aacaaaccag agtatttgaa gcagctgctg
     4261 cctttgcaag gtatgaaacc atactcccgg caccagagtc ctcccatgct attcgtgcag
     4321 tcatagatga ggctttgaag tgcagggaaa ccggcgaggc taagacaata ctcttcgggc
     4381 ttacaggaac aggttacttt gatatgcagg catatataca gtataacagc ggtaaaatgt
     4441 cagactatat cccaaccgat gaggatcttc aaaaaggctt cagtgaactt ccaaatatcc
     4501 ccggacttta atatatcgtc aaaaataagg gcttgaaaat ttcaggccct ttatacttgg
     4561 cagctttgcg cattactaaa ccattttttc gtataactcc atatattctt ttgccgactt
     4621 gctccatgag aaatcctgct tcattccgct ttttacgatt ttttcccata tatacttatt
     4681 tttataaaag ctaatagccc tcttcaccgt atccagcatt tcatgggcat tataatttgc
     4741 aaaggaaaag cccgttcctt cccctgtaaa ttcgttatat ggcattacgg tatccttaag
     4801 cccgcctgtt tcccggacta tcggcaccgt tccatacctt aagctgaata tctggctaag
     4861 gccgcagggc tcatataatg aaggcatgag gaacatgtca gaacccgcat aaatcctttg
     4921 agccaacacg ccgtcatatt taatgttggc tgagaccttg cccggatatc tctcagctgc
     4981 atttctgaac atattttcat aattgctgtc tcccgttccc agcaccacca attgaatatc
     5041 catctgaagc atctcatcaa gtacatgggc aatcaaatca aagcccttct gccccaccag
     5101 ccttgacact acacctataa caggtacttc ttccctttgc ggaagcccaa gagactcctg
     5161 cagcatcttc ttattctcgt acttcccttc taaatcttcg gcattaaagc ttttataaat
     5221 ccttgtatca gtctccggat cgttctccag atagtctatc ccgttcaaaa tgccaaaaat
     5281 gctctctgag cgtttggtta aaatgccatt cagattctcg ccgaagaagt cggttttaat
     5341 ctcctctgca taggtcttgc tgactgtagt gactatatct gaatacgcga tgcccgcttt
     5401 catataattt acgcagtcgt gcagctccaa tccttcagga ttgaaataat cccagctcag
     5461 tccaaggatg tcgccaagga cttcctttgg gaacatgccc tgatacttga gattatgtat
     5521 tgtatacaca gtcttgatat ccttaaggaa tccaatatga ccgaagcctg ctttgagaaa
     5581 tgcgcttaca acacctgtct gccagtcatt gcagtgaata atatccggtt tgaaatcaat
     5641 cagaggaagc accttgagta ctgccctgct gaagaacgca aattgttcgg cttcgtcaaa
     5701 atgtccgtac aggcagtccc tgttgaaata gtattcattg tcaataaaat atgtaataac
     5761 cccctggtat tcaagcttta gcacagcaca atactgatgc ctccaagcca ggtccacata
     5821 cacatagcct atatactcca tcgcttctac aaaattccgc ggaatggatt tatatttagg
     5881 tataatgagc cttacatcag ctccgagctt gaccaactct cgaggtaaag tacctgcaac
     5941 atctgctaac cccccggttt ttgcaaaagg tactgcctca gaagctgcaa acagcacatt
     6001 aatactatcc atgtcctttc ctccctaatc agcctttatt gctcagttat gctcaaagcc
     6061 cttataaccc tttcagaaca attctgaacc agttcactgc cctttttcat cgcttcatca
     6121 atacacatag gcttgtcaat tatggaaaaa atactggcaa agccgttatc ataaagtact
     6181 tcggcaccct tgcctatact gccgcatatt gctattacag gtattccgta tttctccgcc
     6241 gccttagcca cgccgaaggg agtctttcca tatatagtct gattatccat ccttccttcc
     6301 cctgtaataa ccagatcagc atccctgatt tttttctcca gctctacagt ctcaataact
     6361 atgtccacac cctttttaag tgtggcacta agaaatgcca gaaggcctcc tcccaatccg
     6421 ccggcagcac ctgcccccgg atagtccttt attttaactc ccaaagtact ttccaccaaa
     6481 tcggcgtaat gagccaggtt tctgtccaat tcctcaatca tcgcatcatc agcacccttc
     6541 tggggtccga atacctggct ggctcctttg tggccgcaca gggggttgtc tacgtcgcag
     6601 gcaacggtta tcacagtatc ctttattctt ggatcaaggt tattgatatc tattctgtag
     6661 aggctgccaa gacttcctcc cccaaagcca atatcattac cgaagtcatc caggagcctt
     6721 actcccagcg cctgcattgc tccggctccc ccatcatttg ttgcacttcc gcctattcct
     6781 attattattc ttctgcaccc catatccagg gcatgcctaa taagctctcc cgttccgtag
     6841 gttgttgcta ttaaaggatt ccgttcctcg ggtttcacaa gtgggagccc tgaggctgct
     6901 gccatttcaa tcacagctgt acttccgtct cccagtatgc cgtaaaatgc ttctacttca
     6961 ctgagcatgg gccccttcac tgttacggat atcatgtgcc ctcccgttgc atctaccatg
     7021 gactgcactg taccctctcc cccgtcagcc ataggtacct taacaacttc tattgaattg
     7081 tcatatctct ttatgcctgc ctccatggcc tctgctgctt ccttggccgt aaggctgccc
     7141 ttgaaggaat ccggagccgc aactattctt ttaatcattt tatggcctcc ggattttcac
     7201 atatcacagt catatcaaat tgttctctta ggctttccat tttgtacctc caaatatcat
     7261 cttgttcatt tcattctttt ttcttttgta ttattatatc aaaaggaatt aatataacac
     7321 aaggtcaatc tccggcagcc ataatcccca gcgctccttc ctcggcgatt atatcctgaa
     7381 tttgatacac cgatatgggg aagtactgca aacccatgta atagggtgtg atttcataca
     7441 actggaaaaa aagaaccagt gatttatccg caatgtagaa atcctgatac ggccttatac
     7501 ctttgaattc atccaacaga tctatgttcc tgtccgttat ctgctgctca acaatcccgg
     7561 acagcacctt gtcataatct gcttccggct tgaataaatc ctttaattca atgctgtaac
     7621 cgctattcac atcaaaggta agccctttaa ctattgttaa cccgtgagct gagggatatg
     7681 tgaaggcata attacctatt gtcaggctca gaatacctct ctcatttgtt tttatctcat
     7741 accagcccag catctcggtc ttctgctttt cataaaatcc ttgctccctc ataagcatat
     7801 ttactgccgc agctattgtc atgtttattc tatgctgagc agcaggactt tccaagcctt
     7861 ttacaaccgg ataatctata gtcaccatat tatcttgagc tgtaatcctt cttgtggata
     7921 tgcgcaccgg attctgaaca aagctcataa tttaccgccc ctcccttatc actccgcagc
     7981 ccagcctctt gcccgcattg cccgcaggct gggatctgta gtcatcaggg ctttcatgga
     8041 ttatcactgc ttttcctatt atgtcccggg ttttgaactt atcggtaaaa aaacacatcc
     8101 ttgccatacc cttgttggag aaaagcaccg gaaaatctcc cgcatgattc ccatggggct
     8161 gattcgccgg attccaatgt cctccggctg ccttgaaagg ctcttccgga tccccaacct
     8221 cacagctgcc attttcatga atgtgaaaac cgtgaggccc caccggctgc cctccatcct
     8281 tggccgcctt ataatcggac aagccgctta catgtacgca cacccagact cctccgggta
     8341 catttgtgaa tagtactgtc cccttcaacc gttccgcaag gggaccgccc ttaatatttg
     8401 caatggccat ttgtgcattg tacacccatc acacctccag aacagtataa ttcatattat
     8461 tcatacggtc cggaaagtgt gctgtttttc atcactcctg ctcttcgtag aagtccaggt
     8521 aggaatactg ctgtccaccc ttgtgccagc ccaatatggc ttccagattg acattctgtg
     8581 tggcccttag tatgttagta tctgcatttt ctatcttttc ttttaattcc tttatcaaag
     8641 cttttatttc gcagcgcttg ttggctatct tgcctgcggc taccatgcag gcgcagttca
     8701 aaggccagat accgctgctt tgtgtaaacc tttctatata aagctcatcc acatagtaaa
     8761 gaggccttat aagctccagc cctttgaaat tcgttgactt cagcttggga agcattgtct
     8821 tgaagcttcc ggtatacaga atgttcagca tggtagtctc tatcacatcg ttgaagtgat
     8881 gtcccagggc aagcttgttg catcccagtt ccattgcctt gccgtaaagt gctccccggc
     8941 gcattttggc acacatatag caaggataat cctttgcaat tttatcaact attttaaata
     9001 taccggattc aaaaatatga atgggtattt cgaggtaatt acagttttct ataagcaatt
     9061 cccttatatt ctcatgatat ccgggatcca ttgctatgaa ctcaagttca aaggaggcat
     9121 ccccatgcct ctgtaattcc tgcatgagct tggccataag catactgtcc tttcctcccg
     9181 atacgcctac ggctatccta tccccctcct taacaagctc aaattccttc acagctttca
     9241 taaatctggt ccatatgtgc ttcctgtatt tttttataag gcttctttct atttcgggaa
     9301 gcggtttcct ctcactgaaa ggaaccagta tttcacatcc ctttcctgca atatcactca
     9361 tttaaccaac ctgccttatt ataacgatat gtactatact tttctttgat tcatatgata
     9421 cgaaatacac atcaatataa tgcaagaagt ttttaaccta tttcgcagca tatatcatta
     9481 cagtatacat tttatcagca aatgagatat aattaaatca aatatttaat ggaggactta
     9541 tcatgctgaa aattcccgaa agaattcacc tggcaaatct gccgacacgg atagaaaaat
     9601 tggataagct tacaagtgta cttggagggc cggaaatata cataaaaaga gatgaccaga
     9661 caggcaccga gctttccggc aataaagtca gaaaattaga gtactctgtt aaggaagccc
     9721 ttgatatggg ctgtaattac cttattacct gcgggggtgt gcaatctaac cattgcagag
     9781 ctaccgcagc ggctgccgcc aagctgagaa tgaaatcctg ccttgttctc aggggcgatg
     9841 ccggctccga agcagagggg aacctcctta ttgacaggct gctgggagct gatatccgtt
     9901 ttataaccgc tgaggagtac agcgatcaaa ggacggaaat catggaagcc ataaagcggg
     9961 aaacggatga taaaggcttc aaggcttaca taatccctga gggggcttcc aacggaatag
    10021 gaagcttcgg ctattacacc gccatggaag agataatcga gcaggaaaaa gagctgggaa
    10081 tacactttga ttgtattgtg cttgccacag gttcgggagg cacctactca ggtttgtggc
    10141 tggctaataa gctgctcggt cattcttccg acgtttgcgg aatttgtgta ggcggagatg
    10201 ccggatattt caaggcaata atacctgaaa tactgtttga aagcatggag tacatggatg
    10261 ttaagattcc ggtttctccc gaggatataa atattataga cggttatgtg ggaagaggct
    10321 atgccttaag ccgtccggaa gaactcagat tcattcacgg ctttgcagag ctggagggaa
    10381 taatacttga tcctgtatat accggaaagg caatgtacgg gcttaccgag gaaatcaaga
    10441 aaggcaaatt caaaaattat aaaaatatcc tgtttattca taccggggga cttttcggac
    10501 tcttccccca aggagacctg tttaagtttt aaatcctttt atcaatatac tttccaaaat
    10561 ggattcccat ggattttctc atattatcca taaaataatc ggtattcttg ttatcggctt
    10621 ttgtaattgt agtggtctta ccccctgaag catataccct tccgcattcc ggacacacat
    10681 cagtaaatac ccggacagac tgggacacta cctcccgtcc ttctgcccgc gcctttgcac
    10741 cttctcttga aacatgctcc tgctcgtggg aagctaccgc tgccattgag ttttcaggag
    10801 acacatgggt aggagtctta aaggatactc cggcatcatc agatccgtcg acatacttgc
    10861 ggttactgca ggtttggcat tctactgcgc caattttctt catcagcttg tcatttcgga
    10921 tactgtctat tgtctgcccc tgctcaacgg atttcttgcc gtcaggagac atagcggctt
    10981 gtccggatgc tcccgaagca tatccgctgt tttgcgatat taaattgttg tcaaaaccaa
    11041 cccgaagaga cataaatatc ttcccttttc caaattattt agattataag tattttaaca
    11101 tattttcgcc ttttattcca tatagaaatc cggctccatg ttaaaaaaga atactccaac
    11161 tcctcctatt cccaaatggc tggccagggc agctccgatt tcgtttatta caaagttctt
    11221 aacttccagt ctctgtttta tcatgtctct catttcgtac gcaatatcct gatcccatgc
    11281 atagcttatg cctattgtct ggtcaggaaa atccttaatc ctctcttcca tgatatttac
    11341 aatggaatcc agtgcctttt tcctgcccct gaccttttcc aggatttctg cagctccgtc
    11401 ttttacatgc agaatcggct ttacattgag tatactgcca attatccctt cggtcctgct
    11461 tattcttccg cctctgataa gccagctgag atcctgtacc atgaaaatat gttccacatg
    11521 atcagccagc tcacatattt gtctcacaat catatcgaat cccaaccctg ctcttgcaag
    11581 ctttgctgcc tgaagtgcaa tcaggccgat tgccgttgag ccgctcttgg aatcaatgac
    11641 atccatcctt actttgtcat actgtccctg aagctcttgc atcaaagaca cagccaactg
    11701 ataagtcccc gagaactttg aggaaagagc aatatatatg aagtcattcc cttcctggca
    11761 gcactgtttg aaatggccgt atatctccat aggcctcggc agggaggttt gcggaataat
    11821 accctttttc atggcctcat agacctcttt tgcatttatt gtgaccttgt ccagatactc
    11881 cctgttatta atataaactc tttgggatac tgactttatt tcatattcct ccagcagttc
    11941 atccggcagg tcacaggaac tgtctacaat aactcttacc atgtacgaac cctccaatta
    12001 gaataatgct tgtcaactta tcctaattca atatatgtac atggtatccg gtgtgactca
    12061 ctttgtcctg ctgcttggtg aatacccgga acttgtatcc ggagtatttg acaaggaact
    12121 accccatttt ataaaaactt tgatattcac atttgatgag ttatggtata ctacattata
    12181 aggttataat tatgagataa taatcatcag aatattctct tacttattcg tttgatatgg
    12241 gaggttttta tgagcttaaa ttcacttaaa gaattgaaca taggcaatct aactgcaaga
    12301 ctgcctatta tccagggagg catgggagta ggaatatcac tgtccggcct tgcatcggca
    12361 gtggcaaatg agggcggtgt aggcgtcatc gctgctgcag gtataggaat gctggactat
    12421 gatttttcaa caaaatttat tgaagccaat attgcgagat tgagaagcga aatcagaaaa
    12481 gctcgcgaaa aatcaaaagg catcatcggg attaatataa tggttgcttt gtccaactat
    12541 gccgaatttg taaaagcctc cattgaagaa ggaatagata tcattttttc cggcgccggc
    12601 cttcctttga accttcctca gtatctggaa gagaaatatg aaaccaagct ggttccgatt
    12661 gtatcttcgg cacgagccgc aggtattatc tgcaaaaaat ggctggacaa atacaactat
    12721 cttcccgatg caatagtagt ggaagggcct aaggccggag gccacttggg gttcaaggag
    12781 gatcagatat ttgatcctga ttattccctt gaaaaaatcc tgcctgaagt agtggatgaa
    12841 gtaaaagcct ttgaagataa gtcgggcaga agcattcctg ttattgccgc aggcggaatc
    12901 tattcgggag aggatattta taatatcatg aagcttggag cttcaggagt gcaaatggca
    12961 actcgctttg taacaacaga agaatgtgat gcctctatgg atttcaaaaa agcctacatc
    13021 aattcaaata aagaagacgt ttcaattatt aaaagccctg tgggaatgcc gggacgggca
    13081 ataagaaatt cctttaccga ggatattttg aaaggcgcca ataagccgta caagtgtcca
    13141 taccattgca tagtcacttg cgattacaag aacagtccct attgcattgc tcttgcgctg
    13201 acaaacgcac aaaaaggcaa gcttgagaat ggctttgcct ttgcgggtgt aaatgcgtac
    13261 agggcagaca aggtcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg
    13381 agaatggctt tgcctttgcg ggtgtaaatg cgtacagggc agacaaggtc ataactgtca
    13441 aggaacttat ggatacattg cagaatgagt attctgaagc ggcaagtaaa gaagccgggg
    13501 attaataacc ccggcttcgt tcaattgcaa attggacaaa gcctttcagc ttcccgcatc
    13561 ttccaaaata aactcggtat catccctgtt tcttttaatc agattcttca tatcagacat
    13621 tatgcccatg cagcctgcac ctttcacagg cttaccccca aggctgctta tcctctcaga
    13681 tatcttggaa gcatgcttct catgatcctg ctgaatattc tgcagcttct gccttacatc
    13741 cgaatctttt gtcctctcta taaacttttc atatgactta acagccacat actctcccat
    13801 taaatatgca tttaattcct ttaccacctt tttatccata attatctctc ctaaatattg
    13861 ctttatatta ttttttccta tatataatca attatcctta agcgataaca tccggcgtaa
    13921 atcactattt tgacaataat ttcagtaaaa aatacccgtc agtatttact ttgttaaagt
    13981 ttggtgataa aattattttg taaactgaat ataatcttca gggcagggtg aaattcccga
    14041 tcggcggtaa tgcagccaat aagctgctag cccgcgagcg aaagctgacc cggtttgatt
    14101 ccggggccga cagtatagtc tggatggaag aagatatgct tttaacatgt gacattccct
    14161 gagattattt tcagggaatt ttatattttt tcggaggtct tataatggaa aacaaaacaa
    14221 acaaacttgt aagaattgcg gtgctttcag cactctcctt agtgctggta gtgctggtca
    14281 aattccccat cataccgtcc gcaccatttt tggagtatga accgggtgat gttcctatgc
    14341 tggtggctgc attcatgtat ggccctgtcg caggattggt gatgacggta atagtatcag
    14401 cgattcaggc atttacggta agtgctgcat caggatgggt aggcatggta atgcatatca
    14461 tagccacagg cacccttgtt gtagtatccg gtgccatcta cagaaggttc cacacattca
    14521 agggagcttt tgccgctttg gctgcaggct cactcggaat gatccttgtt atgataccaa
    14581 tgaacctgtt ttttacggta aagttttacg gggttcctta tgatgcggtt gtagcaatgc
    14641 ttccgacagc tatactgccc ttcaacatca tcaagtccct ggccaattca ttgattgtaa
    14701 ttctggtgta taaaccatta agcaggttcc tgaagggtga aagcaaaata caaaagaaag
    14761 ctgcataata agaagaatgc gtcattgacg cattcttctt ttgcacagca gcttaatttt
    14821 atataacaga actaaatttg catatcacca attccatgtt tatatatccc gccccattct
    14881 cctatggggt tgttcttatt ttttgaatgc tctgctcatt tttttatcgg aagggttttg
    14941 tatatccttt tgaactgaat tccatatctg tatgctgccc tcttcccagg aaagaatcct
    15001 gtcaggttca ttcgctgcaa aaccggaagg cagtattaca cttgcagttg tgcctacatt
    15061 gggagctatg tctacttcct tttctacgcc gttcactttt attgatttct gcccgttcca
    15121 caattccaca gtcattccgt tccaatggat ggttgccttc tgctctgcag cgttccattc
    15181 ataggttccg cccatttctt ccagtatagc gccaataggc atgttgctta tattaagcac
    15241 ggtcttcccc cttaccacag ctgaatcaat aagccctccc tgtgaaacct ccggctgtgt
    15301 tgcggcatcc ggtgttgtgg ctgctgatga tactatctgt tttccgttct ggtacagatt
    15361 ctgaaaattc cccgtcatgt acatcagtac cagcaagcgc aacgtactcc cataaaattc
    15421 attttaagcc cttcgtatac ttcaggatct acatcttttg caacgcctat tctgtgttca
    15481 tccgcccact gccgtgcacc ttcttctttc aaagtaaact ttgctttagg cgccttacac
    15541 tgaggacaag cctcgggtgc ctccggcccc tcatgaatat acccgcaaat tgtacataca
    15601 aactttttca tataaacccc tccataaata tttattattt attgataatt attatcttgt
    15661 tcataatgtt accatatatc ttctgcttaa gcaagggttt ttatgaaatt ttttaacaat
    15721 ttatatctgg accttacagt accgattgta atcaatggaa ctatcccata caagtgattc
    15781 gctaaaaaat agtgtaaaaa aagtctataa atgataaaat aagtaggggc agtcaaaata
    15841 cccctgctta ttcgtctgta aggagaatta aaatgggaaa aacactggta ttagccgaaa
    15901 aaccctcagt agggagggat ctggcaaaag tattgaactg caatcaaaac agcaacggat
    15961 accttgtagg gcagaaatat atagtaacct gggctctcgg ccacctggtt actttagctg
    16021 accccgaagc ctataataaa aagtataaga actggaacct tgaagacctg cccatgctac
    16081 cggatagaat ggagcttgtt gttatcaaag agacctcaaa gcagtacgga attgtccgca
    16141 gccttatgaa gcagcaggat atcgacgagc ttgtcattgc aaccgacgca gggcgggaag
    16201 gagagctggt tgcacgatgg ataatagaaa aagcaggctt cagaaagcct ataaaacgtt
    16261 tatggatttc atcacagacc gataaggcca taaaagaggg ctttaatcag ctgaaacctg
    16321 ccaaagaatt tgacaacctg tacaaagcag cccagtgcag ggctgaagcg gattggttaa
    16381 tcgggcttaa tgtcacccgt gccctcactt gcaaatacaa tgcccagctg tctgccggca
    16441 gagttcagac cccgacattg gcaatgatcg tacaaaggga aaatgaaata aagagctttg
    16501 tccccaagga ctattggaca attgaagcca aagtcaacgg gtttacattg ctttggaagg
    16561 ataaaagcgg caatgcccgg ctttttgaca aatcaaaggc ggaagccatt gtctcaaagc
    16621 tgaccggtca gacagtggag ataactgata taaagaaaga agcgaaaagc gagccccacc
    16681 cgctggcata cgaccttacc gaattgcaga gggatgccaa caagcgctac ggctattccg
    16741 ccaaacagac cctttcaata atgcagtcct tgtatgagag gcataaggtg cttacttatc
    16801 caagaacgga ttcaaggcac attactgccg atattgtgcc gactttgccg gacaggctga
    16861 agagtatagc cataggcccc tatgctgcaa tagctcaaaa cctgcttcgg ggcaaaataa
    16921 gcaccaccag ccgtctggtg gataacagca aggtcagcga ccatcacgcg attataccaa
    16981 cggagcagcc tgtcaggctc tctgccctga gtaatgaaga acgcaacata tttgatttgg
    17041 ttgcaaagcg attccttgca gttttgagtc ccgcttatca gtatgaacag acaaccgtaa
    17101 aggcagaatc atcgggagag cagctgattg ccagaggtaa agtgacaaaa tccctgggct
    17161 ggaaggctgt atataatgac aggagcaata ccgacagtgc gaatgatgac gaggacaagg
    17221 aacagtccct gcctcccatg caaaagggcg acaagctgaa gctcttatcc gtaaaatccg
    17281 taaacgggaa aaccaagcct cctgcacggt atacagaagc tacacttctt actgccatgg
    17341 agcacccggg taaattcatc gaggatgagt ctttgaggga agctctggac agaaccaatg
    17401 gattgggtac tccggcaacc agggctgata taattgaaaa gctgtttaac actttctata
    17461 tcgaaagaaa aggcaaggag atttatccca ccgcaaaagg aatacagctt atagacctgg
    17521 ctccctcgga gctaaggtct gccgaactga ccgcaaaatg ggagcagcag ctttatgata
    17581 taagcaaagg gaaagccaat gcagatgttt ttatcagtaa tataaggtcc catgccggga
    17641 agttggtttc ctcagttgca gcaagtgccg aggcatacag gcacgacaat atgacaagaa
    17701 cccgctgccc tgaatgcggc aagttcctgt tggaggtaaa cggcaagaaa ggcagaatgc
    17761 ttatttgccc ggacagggaa tgcggctacc gaaagggcgt tgcacagctt tccaatgccc
    17821 gctgtcctga gtgtcataag agaatggagc tgaagggaga aggcgaaggc aggctcttca
    17881 cctgcagctg cggtttccgc gaaaagctgt ccagcttcag caagcgcatg gatgaaaaaa
    17941 ccgaaagcag cgataagcga acggtgcaaa agtacctgca gcagcagaaa aaagaagagc
    18001 ctataaacag cgctatggct gaggctctgg tcaagtggaa ggaaatgcat gataaataat
    18061 aatcgggcgc ccttgtaagg tgtccgatta ttattctttt atgcctttat ttccttttcc
    18121 tttatatact cctcaaatct caactgtact gtatcacaaa ctgttctttc cttgaacatt
    18181 tgttctcctg caggaatact gaggttcgcc tttctgtctt ccgtcatgaa gccttcagtg
    18241 tacagtacct gggaaccata aacattaccc ctgctgcaag gaacatatgg ctcaattccc
    18301 tctgtcaaag cggcaaataa tccgctgtac tgcttaacct cattggatat cttctcctct
    18361 gcttccttaa cggtatcaat cataacaccc atctgtgcac ccagccttga aagtacatca
    18421 aaggtggttt ttccagttaa tggtttaact gcagcctcaa gtctctgtac cttcctgtct
    18481 gaccttgtat aggttccttc cgactctgct gcgctgcaga caggtatgac aacatctgca
    18541 agcttcgcgg tttccgtaat gtaaagatcc aggcaaactg tgaattccaa tttttcaaga
    18601 agctccttga ttctttcatc tgaagcagcc ggatcttccc ctattattac cgcacctttt
    18661 atctctcctt tttctatact ctcaataagc ttatttgcag gaacagtaaa gcccatatct
    18721 attatgccct gggtattggc cttggaacgg gctataataa tgcccctatg cggcttccca
    18781 atcttttctg taattacagc tgcgtcagca attagcttga ttgcctcttc tgatacggtc
    18841 tcctcatcaa tcactattat tgctttcttg gcttcaccat acatttttgc tgcttttgag
    18901 gcatcttcat cattgggggc attatcaaca tactttgcta gttcatcata gccaatggat
    18961 atcttatctg ccttatcctt gtccgcaaat ttctgatcta ttaaagattt aacgaagctt
    19021 ttcaggaatt tgatgttatt ctcaatcttt agtgcaacat cagcatgctc agccaatctt
    19081 gtatcggaat tgctgattga taccagcttg gacctctttg ccgcgtcctt aatctttata
    19141 cccagaacag gatggttttc cgcaacatcc cccacagtta taatgaggtc tgtgttataa
    19201 agctcatcat agctgttggc ggaagcattg cagcccaata ttccctttat tccggactct
    19261 tcttttgccg acaatgttcc aattattccg gtatcaagct tttcggagat tttctttgtc
    19321 aaatatgctt cttcgtttgt aaatttcgga gacatgaaca ctgcaacact gttcttgccg
    19381 tactgcccct ttataagctg cagcttcttg gctgccagcg caattgcatc atcaaattcc
    19441 gcttcaacgg tatcgccgga ccttctgatt attggtttca gcaatctgtt tccatcattg
    19501 atatggtgga ttccaaaacg gccctttgca cataatgtat cttccccttt tgccttattg
    19561 gggcgcgatt tgaatacaag gcttcccctc gactgataga ccacattgca gccaacgccg
    19621 cagtagctgc ataccgaagc cgtgtcttca agctcaacgg gaacctgttt gttaattgcc
    19681 tgcttttcca tgcatgcacc ggtggggcaa acatctatgc aatgtccgca ggatatgcag
    19741 gcactttctt caagaggcaa accgaattcg ggaacaacta ccgaatcaaa gcccctgtcc
    19801 aaaagcccga gagctgttac tcctatgacc tcttcgcagg ccctgaagca caaaccgcag
    19861 agtatgcact tctccgcatt tctttttatg aaaggatggg tatccgcttc ttctttcctg
    19921 tgtatttcac cctttattgc cttggtgtca atatcgtact tccctatata attgacaagc
    19981 ttgcactcaa aatagtcatg acagccgcat tccaggcatc tggaagcctc cttcacggcg
    20041 gtttcttcat caaaggtctc tgaaatagcg ccaaagctca tttttcttat ctttgcaggg
    20101 tatacctttt ggtgttccct ttccttcttc tctttatcgg caaaatcttc cgggcccaaa
    20161 tcttcctgct caatgtagca gggatttgca acaggtttta tttctccctt caggtagctg
    20221 tcgattactg aagctgcgtt tttaccctga gctatggctt ctatggcaat ctttggccct
    20281 gttacagctt cgccgccggc aaaaactccc ggaatattag tctcaaaggt attggcgttg
    20341 gcagcaattg cgcccttctt tgtaagctcc agctctttta cgctttctgc atttactctc
    20401 tggcctattg cagatattac aagatccgca gggattgtca cttcatctcc gggaataggc
    20461 tcgggacttc ttcttccgga cttgtcggga gcgcccagct tcatcctctg gcatcttaca
    20521 gcctttaccc ttccgttccc ttcaataatc tctatggggg caacaaggta agtgaattct
    20581 acgccttctt cttccgcttc ctctatttct atatcttctg caggcatttc agccctggtt
    20641 cttcgataga gtacctgcac atgctccgca cccagtctta ctgctgttct ggctacgtcc
    20701 atggcagtat ttccgcctcc tatgaccaga acactctgtc cgagatatat aggctctgca
    20761 agggtagctt ttctaaggaa ttcaatgccg cccattaccc cttccatatc ttcgccgatg
    20821 cagcccatat tcatgcttgc ccaggcgcct atggcaataa acactgcatc gtaggtcttt
    20881 ttaagatatg ctagtgtaat atcctcacca agctttacat tgcactttat gtctactccc
    20941 atttttacaa gtgtgtttat ttccgcgtct aaaagctctt tgggcagacg gtattgcgga
    21001 atcccgtacc tcagcatccc gcccggatac ggcttgtcct catacagaac cacattatgc
    21061 ccctttgctg ccaagaaata tgcgcaggat attccggcag gccctgagcc tattacagcg
    21121 actgacttgt ttgtaggctc cttaatttcc ggaacataag tgccgttata tatatctata
    21181 tcgcctatga atctcttaat atccgcaatg gcaatgggtt cctccacgtt ggttcttctg
    21241 caggctgttt cgcaaggatg ggggcaaacc cttcctatgc ttgccggcaa tggaataacc
    21301 tctttaacca gctttatggc ttcatcatat tgacgatttg cgaccaggcc cacataaccc
    21361 tggcaatcag tatgccccgg acatgcattt acacaaggag gccggcagtc tcccttgtga
    21421 tccgaagaca gtaaagtaag tgccgtcttt cttgcagcta tagttcttgg ggtatctgtc
    21481 tttattatca ttccgttaac cgcaggtgtt gaacatgccc ttaccagctt tggatttcct
    21541 tccacttcca ccacacacat tccgcatgct ccgtaagtct tgagattctc atcataacag
    21601 agatttggta ttcctatccc gttatccaaa gctattctta atattgtact gccttcatcc
    21661 gcggtcaatt ctctattatt tatattaact ctaaccggtg ccattttatc acttccttta
    21721 ctattctacg tttatcgcac caaacttgca tgtgatggcg caatttccgc atttaataca
    21781 tttttcctgt tcgatactgt gaggctgctt cctttcaccg gtgatagcct cggtgggaca
    21841 ctttcttgca catgcagtac agcctgtaca cttatccttc aggatgctgt acgttataag
    21901 gctcttgcac tgcttggcag gacacttctt atccctgata tgggctatat attcatcctt
    21961 gaaatatttc aaggtcgtca gcaccggatt aggggcagtc tgccccagtc cgcacagaga
    22021 accgtccttg atgctgatac atagctgctc cagttcatcc atgtcttcca tcctgccctc
    22081 tccttttgtg attctctcaa gaatctcaag cattctcttt gtgccgattc tgcagtaaat
    22141 gcatttgccg caggattcgt cgcaggtgaa gtttaggaaa aacctagcca tatcaaccat
    22201 acacgtagct tcatccatta caaccattcc gccggagccc atgattgcac ccgtcctggt
    22261 aattgactcg taatcaaccg gagtatcaat caacgatttc ggtatgcatc cccctgaggg
    22321 gccgcccatc tgaacagctt tgaaatccct gtcgttcttg attcctccgc ctatttcata
    22381 tattacttcc ctgatcggca ttcccatagg aacctcaacc aggccgccgt tcttgatttt
    22441 tccggcaaga gcaaaaacct ttgtcccgtt gctcttttcc gttccgtatg cggcaaaagc
    22501 cgctccgccg tttcttatta tccacggtac gtttgcataa gtttcaacat tgtttatgtt
    22561 agtaggcttt ccgaaataac ccgactgagc agggaaagga ggcttaagcc tcggcattcc
    22621 tctctctcct tcaagggatg cgatgagagc tgtttcctct ccgcagacaa aggcccctgc
    22681 acctttctta atcttgatat cgaagctgaa gcccgagccc ataatattcc cgcccaggaa
    22741 atttgcctca tgggcttggg ctattgcccc ttcaagcctt tctatggcaa gaggatactc
    22801 tgccctgcaa taaataaagc cgtgatttgc cccaattgca tatccggcaa taatcatacc
    22861 ttcaagcaaa ccgtggggat ctccctcaag tacgcttctg tccataaatg cccccggatc
    22921 tccctcatcg gcattacaaa ctacatactt ttcatttcct tttgcttttc ttgcggcatc
    22981 ccatttgaac caggtgggga aaccggcccc gcctcttcct ctaagccccg atttcttaat
    23041 ctcttctatt acctgatcgg gagtcatttc ctttaaacat ttctcaagag ccttatatcc
    23101 gcccttttcc aaatagtcct caattttctc ggggtttata ataccgcagt tctgcagtac
    23161 caccctgacc tgtttttcca gctgtttaaa cggttttttg ccggttgatt gaatatactt
    23221 ctcaacagga acaccgtttt ttatgtgctg ttcaacaatt tcctccgcct tttcgggtgt
    23281 gacgtttaca taggtatata cctcgccctc atcgtctata acatcaacta taggctcgta
    23341 gaagcacatc cctatacatc cggtggatgt tatttctata ttcatatcct tttctttcaa
    23401 gcagtttaac agggcttccc tgactttctt tccgccggct gcaataccgc agctgccgag
    23461 acctatcctt agcttcaatt ggaagcaccc cctttttcct ttttgtatat acctcttatt
    23521 acttctcttg cactgttagg tgtaagattt ccataggctt ccccgtttat catcataacc
    23581 ggtgcaaggc tgcagcatcc aaggcatgcc acattttcaa gggtaaacat tccgtccttg
    23641 cttgtccctc ccggttctat tccaagttcg tcgcatatgg cttcgcttat cttgtcagat
    23701 ccgtttacat ggcaagcagt tccctgacat tgaagtatta tatgcttacc tataggattc
    23761 aatctgaatt gtgaataaaa ggttgcgatg ccgtaaatct ttgctttctt gtttccggtt
    23821 ctgtcggcaa tggttttcaa agcttcaaga ggaagatatc cgtaaatacc ctgtgtatcc
    23881 tgaagtatgc tgatcaggct cgcactttgc ccctcatact tcttgaacac gggttccata
    23941 agcgataaat ccacttcctt cttgcagaaa ttagtttcca gttttttttc acaagtgcat
    24001 gacatgagat gtatcccctc ctacatgtat aataatatta gataacccta aaatatatat
    24061 aatatatgtg taaatttact gacaacctca attttgtcag atgctatgtg atgtatccaa
    24121 aaccttgtca tccttcgttt ttgcattctt atcacaggaa aatagcagta ccgaagctat
    24181 taccataata ctgcctattg ccgatgtcaa actcagtttt tcactaaaaa gtataattcc
    24241 cataaccaca ctggtaatcg gagcactggt gctgaatatt gcggcgtacg tggggccaat
    24301 catctgtatg gatttgttgt aggcaagtat tccaaaaacc gtactaatca atgcaaggcc
    24361 caaaatgccc agcactgcag gcagctgcat cgagaacatg agattgcgtg atgcagcccc
    24421 gaatataaag aatgcagcag cagagaaaaa tgacacataa aaagtgatga gaaggccgtc
    24481 cattgccatt aattcctctt tgccgatttc tatagtatat atggaataga gaatgccgga
    24541 tgcaagtgcc aaagcaactc ccttcaaatc caccgaagat cctgtgttgc ctgccagtat
    24601 ataaaccccg acaatagaaa ttgccaaagc tatcatcttt aaggccggca ttttttcctt
    24661 aaaaatgaaa aaagaaagaa ccgctacaac cgccggatat ataaaatgca atgtcatggc
    24721 caaaccaatt gaaatatagt tgtaagacaa aaatactgtt atagtggttg ccgcataacc
    24781 gattattcct atatacgcaa gcctgcaaaa aagcttcttg cccaccttaa gctctacttt
    24841 cctgcagcgc aggattcccg ccaatatcaa agatgcaata aggaatctta agagcaaaac
    24901 agtaacggca ttagtccctg cgttataagc aattaccgca aatacaggca caaatccaaa
    24961 ggatacagcc gaagaaacgg catatataat acccaatgtc ttattactca tgataacctt
    25021 cctttgtata tttttggcat atcagcagtc aataaaatat atattaatta tacacaacta
    25081 ttagcgaatt accaatcata aatttataag tgccggatac acatgttact tgaacctgat
    25141 aacctgtgca cccggcactt attttacttt tcgtttcctg ccgccctttc acgttctctg
    25201 aaaagcaaac tgattgacca tatcccggca acacctacta atgtataaat aagcctgctc
    25261 agcagttcat cctgccctcc aaaaagggat gccaccaggt catactggaa aaggccgaca
    25321 agcagccagt tcagtgcgcc cacaattacc agaataagtg ctaacctgtc catgtgaata
    25381 aatctccttt ctatattaat aataaatatg gaaatattta taacaaaata tattatgcgc
    25441 atttttatga ttgctataca ggtattaaat actttgcacc catgcatatt attgccttgg
    25501 cctgtatctt gccggctaag cgctgggcgc ttaatctttt tttgttcttt tgctctttgt
    25561 ctggcacata atcctttttc atgatataat gttatgtaaa ctataaacgg aaaaaggagg
    25621 tttactgtga agaacataaa atacattcaa ataataaaag gctttattgc actgattatt
    25681 acagtggcct tgttatatac aggacaggca ttgtggcgga gttacaccat tgacttaccc
    25741 ctggacaaaa cgctcaatga aatagacgga gttgaagaag tttcctggga cgccggcagc
    25801 agctttaacg atgccgtaat cataaatatt aagctcggaa atatagacaa tcttcaaaaa
    25861 acctacaaag aaataaaaga aaaaattgac ctgacactaa atggcaaaaa atattcactt
    25921 gaaatagcgg atgaccgatc tcaggagttg gagcaggcat attatgatat ccattattat
    25981 gtccaaaaat ccataataga tggagacttc ccttctcttg aggccgaagt ccgggaaaag
    26041 gctgaaatga ttggggctga agctaaagtt tacgtggatg agcaaaatat ctatcttcag
    26101 tttacaaaga aggacaattc tttatttgcc gcaattggac ggcattttga cagaacaggg
    26161 ggaaatcttt gatgaagaat agggagatct ttgcgggagc agcggcaggt gcgctgatat
    26221 tcctggcgct tcaaagggtg aatatacttc ctgttgtgtt catacttgga ctcatcggca
    26281 tagtatacta tgtgaacttc ggcaggctat ccggcaatag tatcagcttc gcttcagcag
    26341 aaagaaatat ctgttcagtc tcctttgatg atatcggagg ccaggaggtt gccaagaacg
    26401 agcttcgtga agctcttgaa ttcatgaaaa atgacgagca aataaggctt ctgggtattc
    26461 gtcccttaaa gggcatattg ctaagcgggc ctccgggcac cggaaaaaca ctgctggcaa
    26521 aagcagctgc ccggttcacg gattcggtat tcctatcagc cagcggctcg gaatttgtag
    26581 aaatgtatgt cggtgtaggc gctcaaagag tgcgcagtct ttttgaacaa gcaaggaagg
    26641 cggccgaata cctgaaaaaa tccagcgcag taatttttat tgatgaaatt gaagtgcttg
    26701 gaggagcaag aggccagaat aacgggcatc aggagcacga ccagactctg aatgagctgt
    26761 tggtgcaaat ggatggcata tcctccgatg atagaataaa gattcttgta attggtgcaa
    26821 caaaccgtat tgatatgctt gatccggcat tgcttcgccc cggcaggttc gaccgcctcg
    26881 taaaggttga tcttcctgat aaaaaaggcc gtatgcatat actggaactg catgtaagaa
    26941 acaaacccct tgcatcggat gtaaacgtag aagccctggc caaagatacc ttcggattct
    27001 ccggagcgca gctggaaagc gtcgcaaatg aggccgccat acttgcaatg agagacggct
    27061 ccgataagat tgactcaaat catttcagaa gtgccgttga aaaagttatg atgggggaaa
    27121 agcttgacag gcttccaagc aatgaagaaa agcgcagaat agcagtacac gaatcaggcc
    27181 atgcactggt tggtgagctt aggcttcccg gttccgtcgc cagtgtcaat gttgcatcca
    27241 gaagcaacgc tttgggttat gtacgccaga cccaggaaaa cgatatgtat ttgtatacct
    27301 gtgattatct gaaatcaaga atttcggcag cactggccgg tataatcgcg gaagagctga
    27361 tattcggcaa tagaagcacc ggcggttccg gtgattttaa gcatgcctcc gacatagcaa
    27421 aacagattgt atacagcgga atgtccgagc ttggaatcat ctcttctgag gatattccgg
    27481 gagaccagct gcattcagct attacatcca tacttagaga tgttgaaagc agcacccgcg
    27541 actttctttc gaattataga agagagctta agcggatatc agaattgctg ctggataaag
    27601 aaacaatatc gggagaagaa ttgaggctta tattaatttc agagtgtgca tagaacaaaa
    27661 actaaaaaaa accaccttca ggcagcattg cttatctgtt gataatctgc tttgcgctgc
    27721 cgaaggtgtt tttcccttct gttatactta cccattcagg cttcctccgg ttgtataccg
    27781 taatatgcct gaagcctgct tcccttgcca tgtctaatgc ttctttcagg cctctgccta
    27841 catcctctgc catgtgtgca tcagaaccaa tagtcagtat ctctccgccc atattccggt
    27901 aaagcttaac aacatccagg ccgggaaatg cttcttttgg gctctgcctc aatccggaag
    27961 tattaatctc aattcccttg ccgctttgaa cagccaacag cagtactccc ttcaacagct
    28021 catgccggca cgtcaatgag atgttctttt tgtatacggg ggcactgtac cttttaatca
    28081 ggtccaaatg tccgatacaa tcaaagttgt tccagtaaac aagctgctta agctgcctga
    28141 gatacatttc gcatagttct tcttcaccta ttctgctgta gtcaagctct ttaatgtcca
    28201 tccctgcagg aagcttgtgt acagatgcaa tgatataatc gtagggaaaa tctgcaatta
    28261 ctgcacttga tgcttccgaa aacaaatgcg gctggccaag ctcaactcca agctttatat
    28321 tgagtcttcc cttgaatgcc tgcctggctt tattaatatg agcccaatac cccttttggt
    28381 tgtatgacga gccatattca tccgcaatat ttggttcaaa gtgatctgtt attgcaattt
    28441 cgccgattcc attgtttact gcactctcac acatttccat tattgtattg tgcccgtcgg
    28501 atgaatagat ggaatgtaca tggtaatcca ccaaaaaatt cattttctca cctcctttcc
    28561 caatctatga tatcacataa ttgtagtttt tgtattagga atctatgaaa attatgttaa
    28621 ttatttgtta caatgaatac aaaaaaaaat ccatatgctt agcaccggat ttagtatata
    28681 tgaagctcag ctgcagctag tttttatctg cattacgcct gccttcagat agaataaggc
    28741 acacattatg gagatggtcc gcaattcttt ccaaatgagt tatggcatcc aggtagataa
    28801 ccccggcctg aacactgcaa gtaccttcct ccagtcttct gatgtgcttt tctttaaact
    28861 tcagcgtcaa ttcatcaaca acctcttccc ttgcagccgc ttgaagtgct aattccgcat
    28921 catcctttga gtatgccagg cagcattcat tcatcatgct catgacctct tgatacagtg
    28981 tttccagttc atcgatgcct ttctcactga atattatctt tcgatttatt ttactttggg
    29041 ataactctgt aatgtcctca caatggtctc ctactctctc aaagttattg atagtgttta
    29101 taagagcagg tattcttgca gactggtaac tgtcaaggtc cttatgggat atttccacca
    29161 gatattgagt tatttccgtt tgcatgatat ttaacttttc ttcgttctgc agcactgaat
    29221 caattttact ctcattatcc tctataaggc tgatcaggca atcattgagc atattctttg
    29281 caacctctgc cgcatttatt acttcctgag ttgcagcttt caaagcggct acgggagtat
    29341 ctaacaggag ccggtccaaa gtacccgctt catacttctt atcccttctg tcaggtatga
    29401 ttttttccag gaacagcaca tagtattttg taatcggaag aaataccaaa gcactgatta
    29461 tattaaagaa ggtatgtgta tttgcaacaa gaaaagcttt tggctcatgg agtatactga
    29521 aggtaatcca ttctatcatc cccgagaagg gtctcagaac aataatggca attacgacac
    29581 ctataacatt gtataaagta tgtgcccatg cagttcttct agcggctgta tttgccttga
    29641 ggcttgccag ctgcgccgtt acgcatgtac cgatattatc tccaagagtt aatcccacgg
    29701 cagcgtaaaa gcttatcagg tctgcgctga aaagtgatat ggtcaaagca actgttgcgg
    29761 cactgctgtg aataagcatt gtcgctatca tgcccacaaa cagccccagg aaagggtttt
    29821 gcccgtattg aaggaacagc tgcattgcgg tctcgctgtc ctttatgtag ggcaaactgg
    29881 cattcaatgt cttaagcccc aggaacatga ggccgaaacc catgatagct tgtcccaaac
    29941 ttcttgtgct gtctttcttt gagaaggaga agatataaaa gcctattgca attatcggaa
    30001 gggctatata tgtaagctta agggacatca actgggcagt tatagtagta ccaatatttg
    30061 taccgtatat gatacccaca gcttgagtaa gattcatcaa tcctgcattt acaaacccga
    30121 cagtcataac ggtaattgcc gtgctgctct gtacaagcat tgtcaatatt gtgcccacaa
    30181 aaaaagctac ataaatgttc tttgtaagta ctgagagtat tcttttcata acctttccgg
    30241 aagccttttc caatccgccg cccatgaagt cgacaccaaa catgagcaag gcggtcccac
    30301 cgaaaaggtt gaaaattatc tgaaacgtta tactctcctg tatcaagcta ttcaagtccg
    30361 gcataggaat cctccatatt tgctgattag gtatccatta gtattatata tcatctaaca
    30421 atataataat atcactattt atgttttttt gtcgatatgc cttataaaaa acgcgtcgaa
    30481 ccctatgcgg gacacgggga cgtttctcct gtcccactga aggtgggaca ggagaaacgt
    30541 ccccgtgtcc cgcagaaacg tccccgtgtc ccgcgaaaag aaacgccgca ttttattgcg
    30601 gtgttgtatc attccgaaaa gccggctctc ctattataag cttctgggtt gaacccacct
    30661 gtgagaatat gaaaagagta tctaccccac tgatgtcaat atctatctca acagtctccc
    30721 cgggctttag ctggaggctc ttcagaacca caccctgctt gttgtctttc ctgatatcca
    30781 ttgtcaattc ttttttggta tcttcactca ggaatatcga tgcggtaaag cgcttgtatt
    30841 ttccttcagg cttcaagcag cagccgtagt atacgctgtt taaaggacta atgtttgcaa
    30901 ttccccattt gtacgtcgta tctgcggttg taagcttatc tgtatcggta gtcactattg
    30961 tccgtgagta atcctcatac atgctggtat cattaatggg aacgatctgt gttttcccgc
    31021 ccagccatac gattttgttg actccatccc attcaaccgc tattccgaga gcgtctccca
    31081 ggggcttcac cggcagatat gtcctgccgt tatatataag gggcttcaga taagttccgt
    31141 catcgttttt gggagcgaaa tcattgccaa aaagcttcat tttaatggta tttgacaata
    31201 ttgcctttac ttccgtgtct gcaggctctc cgattactgc catcatacct gctgtcagaa
    31261 gaatacagca cagaaaacct gcaaaaaata ccctgatatt cctagacata acaacacacc
    31321 tttctgttta aatttcatag tgtcctacat tatatatgat agataaatga aaggtatgcc
    31381 gctttattat tgttgctgta ctttttattc aaattgctga cttgtcttta aatgttcaat
    31441 tttacaccac tacaacatat ctgcagccgt ttttctcagc tgttttttta agctcctcca
    31501 tcaactcatc tgatgggata ccgacttttt tgatatcgcc ggcaactcct accggcctga
    31561 acctaatcag cttgtaccgt atatccgcat ccaattttgc aatcagcttg ctcacttcag
    31621 caacattacg gtaattgtca agaatccccg gaactataac agtccttatt tcatatagct
    31681 tttgcttgtt tgccaggaat tccgcattct taagcactaa tgcattggac attccggtaa
    31741 gcattctgtg ctctccatcg tcataggact ttatatcaag cattgcctta tccatagcaa
    31801 atgtcagctc cggttccttt gaaaaatcaa tacagccgtt tgtatcaacg taggacgtaa
    31861 gccccaattt gtgcaccgcg tcaaaaagct ccgcaaggaa ttccttttgc atggtgcact
    31921 cgcctcccga tacagttatt cctgatataa aaggcttcac tttctcaacc gttttcatta
    31981 tgtcatttac tgacattgaa acagcctttg gactgctttt gccggggcat gccgcaaggc
    32041 aggcatcaca acctgtacag tttgcggaat tccattctat tcttccgttt ctgcttctta
    32101 acgcacctgc ggggcatacg tccaagcata gcccgcagcc ggtacatctg tttattgtct
    32161 caggattatg gcagtacagg cagttgaaat tgcagccctg aaaaaatatc accgtacgat
    32221 ttccggggcc atccacagag ctgaagggca gtattttatt cacaagtgct tcagccattg
    32281 tgccttacct ttctttccaa tattccggaa ttctgtactg cgccaagtcc cagtgcaaca
    32341 gtgtcatgca gcgcctgctt cccttgctga agtgcctcca tctccgactt cttgacaaga
    32401 tatccggtta ttctgattaa atcactgtct gaagcataga aggatatata cctcaggcct
    32461 tccgaaaaac cgcccttaat aatatcaagc aaataggcgg gatttctctt taccgtctcg
    32521 tcaaagctaa acacatctcc aatccctgat ggaaaatatt tatgaaaggg agcgctctgc
    32581 agcagatgtt catatatttc cggctcctcc ccaatgggta tccggcatcc ggggctgatg
    32641 ccgtaatcgg tatcaatccc aacctgggca tggagcagaa aacggccgtc ggttgctttg
    32701 cagtactgat tgttatgctc cgataccaat tggtgcatcc tttctattat tctttgccca
    32761 agggcattgg cggcatcatt atgcccgaac ctgtcctttt gttcctttgc cccaagcaag
    32821 tgattcacgc attccgcaag cccgaccata ccaaacatgg ctgtaaacct gtctttatat
    32881 attaagctct catccgcaag gaagctgctt tcaaagaaac cgctttcttc aacaataaac
    32941 ctgatgcgct ggtccataaa ctcgcgcatt ttagaaaccg cttcaggaag tacttcttca
    33001 aggaagtgcc cagcgttgtt tgattgtttt gccaatcttg caagtaccag ccttacaaga
    33061 gtatagcttc ctccccctat tggcagcccg ttataacagc tggctattga ataattgata
    33121 ccaaagtcat taacaaacat ctcatgattt gcaaagctgg gttttgcaga tacaagggct
    33181 gtttccaccg cttccaatgc aaattcttca ggagtatcct tactgtattt gattgtcagg
    33241 ttcgggacag catcctgaag ctctttctca gcccttaaaa tgagtcttcc tgcttttgtt
    33301 gcccccgggc caatgttcgc gtggcagaat gaatcggtaa cggtacggtc caagtgcatc
    33361 aggaataacc tgacagcctt atatgcctcc tcctcgtcct taataaaagg ctccagcaaa
    33421 gtatccaggt tccctacata cacaggcatt gtagtaacgg agggaacatg actgtacaat
    33481 atcaacagat tgttcgtggc ttcccatata tcccttgcag gcttcagtct caggaactca
    33541 ctgccttgtc tcataaacct ttcatagtca ggtactatgt aacggggcct gtacggcgcg
    33601 tttccctcat tcaaatcgca tattattcct tctcttctgt attcctgtat ttcttttgat
    33661 atcggaagaa cactcaaggt gttttctgca gcccgggcca agcttactat cttttgctca
    33721 taagtcagtg tcctgtcctt gattatactt aatacttcac tcattttaac accttcaata
    33781 aaaattacat gttagtaatt gttttaccta tattatacca tttattattt tgatatgcta
    33841 taaagtaggc tgttgtacaa aatatgaaga atgcgtcgca ttcttcgggt acgaggtacg
    33901 aggcgcgagg ttcgaggttt agggcagggc ttcggagtta tgataacact gaagacacga
    33961 aaaaccgcta aatgcacggc gggataaata ttacgttaca taaataatta tcgataatga
    34021 tgtaagtatc gaaattttct tatgtaacta tataatatat aaaagttacc gtgcaatcgc
    34081 gcagcgattc catgaaacag ccgtgtctcc cgtgttaccc gttacctacg tcgcattatt
    34141 cttctattgc gaaatcccca ttctatgctc tccagatatg ccacaaatgg ttgttctgcc
    34201 ctctggcgaa tacatccagg cggttgcctc cccaggatac tgctgcggga tctgaggtca
    34261 gcacacctcc aaggggactc caaccgctcc atcgggttcc gttccaggtt ctgatcatca
    34321 gttcatttct ctgccctctt gcaaagactt caaggcggtt gcttccggta gatgccgcag
    34381 ccggcccgga agttatctgc cctccgccaa gatcctccca agcgctccat cgggttccgt
    34441 cccagtattt atgccaaagg gactgattct gccctctccc gaatacgtct attctgttcg
    34501 gcccccagga tactgctgca ggtgctgaag tcagcactcc tcccagatcc tcccactcgc
    34561 tccagcggat accgtcccaa tatttgtgcc acaaggattg attctgccct cgtgcaaata
    34621 catccaatct atttgcctgc caggatgcag ctgcaggttc ggaagtaatg atgcctccaa
    34681 gatcctccca tgcactccaa cggataccat cccaataaat atgccacaat gcctgattct
    34741 gtccccttgc aaatacatct atcctatttg ttccccatga tacctctccc ggccctgttg
    34801 tcagaatccc gcccaggtct tcccatgcac tccagcgggc accgttccaa taaacatgcc
    34861 acaatgcctg gttttgccct ctagcaaata catccagacg gttaggctgc caggatgcag
    34921 ctgcaggtgc ggaagtcagt attcccccaa ggtcttccca ttggctccag cctgtacctt
    34981 cagctacttc aatattttcc agcgtaaatt gaattatatc cctcattatt tgatttatac
    35041 gggcattagg aactcctttt cgtctaagta tggtgaaggc atacggatta tcccgtcgaa
    35101 gcgcattcag aagtgcattt gttctctgct ctatagtccc attataattg tttgcatttc
    35161 tcaatacata tattactgtg tttctaaata tgttacgcgt atctgaacgc cgcaccccgt
    35221 acctttcaag ctcttgatag tggccggcaa acctcctgtt aataagcggc atgatgcggc
    35281 tgacatcctg cggcctttgc ctcatacctt catagccata ctcgtcatat tcatcctcaa
    35341 agtacaattc atcgtaatac acttcaatga aggagtcatc atactctgta tcctccgtta
    35401 aattttggaa cggacagatg taaaacggat tcatagggca catataagga ttcttgtaca
    35461 atttaattcc tatctccttt ctccacaaac gtatcattac atgatatgca gcataactcc
    35521 gaatggttaa aaggaaatgc agatagaaat gcactgtatc cgttatgaag atacagtgca
    35581 tttacttttt ttatcaatca ttttcctctc cggacgtatc tctaatttgc tatggcatct
    35641 tcaaaccgta cataattcag cagcttgctt tccttataca gccataagcc tacaaatcct
    35701 tcatcaataa gttttctctg aagttcaaaa tcaccgatag gtatgccaaa attctggctt
    35761 acttttctta ccgcccttgg ctccagattt ctatgaagaa taaacctcca cattccgtcc
    35821 ttcccatctt tttctgaacc gtactttttc ttaagtgctt tgattctcaa tccgtttttt
    35881 ataatattgt aaaggctgta gatattctgg ggtgatacag tacaaagcaa atgactgtac
    35941 ccaaaatcct taatcattgg tatggcttgc tgcaatgtca gactctgcaa tgagttgcct
    36001 ctgtattcgg gatgcacaac tgtcgtttca agatgagcta cctttgccaa ttcttgcctc
    36061 ggcatattaa tatcattacc aagatttttt tcagagcatc ccgggaaacc tatatacctg
    36121 tatgcaataa tatctcctgt tttgttgaat actccgatta tcttcgctcc ctgtttcata
    36181 tcatcataca tttcttcata agaatccatg tacagaacct gcttattagg aagctgctca
    36241 tatacatatt tctggagtgc aactgcatct ccaagctcct ccggagtaat tatcctcata
    36301 acgtattcat cttgttcctt gctttcattt atatgcctgg tcagttgtcc ttttttcaat
    36361 atagtcatat gctagcctcc tttcaaaatt ctctgactgc tgtacaagta gttacatcat
    36421 ctgacaactt atgcaatttc tttaagttgt ctcttttatc taatattatc acaggcaact
    36481 cgtgtttgtc aagcaagttg tcagatattt cttaaaaaaa atattgcttg caatgaaagc
    36541 aaaaaaagcc tgatatgaaa ccatatcaga cttaagtttt tactttacat atttagcaac
    36601 tttttcagat atctccgcat cgcttcccac ataaagaata atgtcattgg gaaagaacac
    36661 catcttgggc cccggcgaaa gatacaggtg cccggaacga tttattccta ttattgtcgc
    36721 ccctgtgtga ttccagaaac gcacttcgcc gatggttttt cccaccaagt ggcttttttc
    36781 aggtattttt agctccaaag gaaatattat tccaatattt ctaagaagca tcgcctgttc
    36841 aataacggaa tccaatcgtt caagaacctg cttttcaagt gcctgctttt cctccattaa
    36901 tttgacaatc tcttcacgca tggaaagtat tctgtccttt gattgatgtt cttttatgaa
    36961 gttattggct gcatcttttg attttattaa tatacctttt ttttctatta catttacaac
    37021 ctgcagttct tccaacagct tcaatgcacg tcttattgtc tccggagaaa cgccgtattt
    37081 accggaaagt gtagacctcc ctttaaccat ttcgccggct ttccattcct cattgagaat
    37141 actgtaagct atatccagtg ctatcttctc atattttgca gccggtattt tatcttcccc
    37201 atggcttttt agattcattt caacaccctc tgaattgtaa atattggtat taattattcc
    37261 ttactataat tcttgtattt tttacaccaa ttgtcaagca tataatatga cctggtgttt
    37321 tagttctgtt cctgcaccac agcgcctcgg tgcctggcgt ccagattttt caattcccta
    37381 ataagaaaaa ttcctgtaat gaccgacgat atcaggtctg acgcaggccc tgccataagc
    37441 agtccttcca ggccgaaaaa taacggcagt ataagcagtg ccggtatgag aatcaatact
    37501 tgccgtgaaa ggctcaggaa tgccgcatgc ttcggtttgc ccaccgcctg aaaataattt
    37561 gccgatacaa tttgaaatcc gataatcgga agaaatatca ggtagattga aatcgctctt
    37621 gttccaaaat tcaataaatc cttatccgtt ctattgaata ataatatcag ttgtgacgga
    37681 aatactcttg tgagtataaa tccaatagtt gttataattg ttgccgcagc aactgcaagt
    37741 ttcaaagctt tcttcaccct gtcaaactca agtgccccgt aattatatcc tataatgggc
    37801 tgggcaccct gattgatacc aaatatcggc ataagtatca gcatagcaat actcattata
    37861 acacccattc ctgaaacggc tatatcgcct ccgtatatta caaggctttt gttcatgatg
    37921 gcattcagga cgcttgcggc catctgcatg aagaatgttg cagctcccag ggaaacgatt
    37981 ttaactacta tcggcgtatg cagcctgagg ttttttgtat gtacctttaa ggtgcttctg
    38041 ccgccaaaga aatgatacaa cacccatgac gctgatacag cctgtgacaa aacagttgca
    38101 atagctgcac ctctaatgcc caatccgaat acatagataa atataggatc caatatggta
    38161 ttcagaatcg ctccgattat catcgtaaac atagcaattc tgggattccc ttctgccctg
    38221 ataaagttgt tcatcccaaa gccgacagtc tgtataaccg ttcctaaaag tattatgctt
    38281 aaatattctt ttgcgtatgg aagtactgct gcgctggcgc caaaaatcct taacagcggt
    38341 tcaataaaaa tcagacctgt accggaaatt gtcatcgatg taataacaag cattactact
    38401 gcatttccta aaatcagctc cgcctcttcc ttcctctgct ctccaagcct tatagaaatc
    38461 agagagttcg ccccaattcc gataagcatt gcaaaggcca taataacaag cataatcgga
    38521 aagccgatgg ttatccctgc tattcccagg gggcctacgc tattccctat aaaaatccga
    38581 tccactacat tatataatgc atttaccagc atcccgacaa tcgcaggaat tgaaaacttc
    38641 agcagcagtt tggatatctt ttcttcgccc aattgtttag atgtgtccat atattaactc
    38701 ccactccaaa aataatatgt ctttaacaac aaccttcttt tataatacag tatctccata
    38761 atactaatga taagtttaaa aatcaagaaa aacactaaaa tagagacatg gggacgggga
    38821 cacggggacg tttcccctgt cccgctgaag cggggcacat gcggctatag cgcaaaatat
    38881 gaagaatgcg acgcattctt cgggtacgag gtacgaggcg cgaggttcga g
//
LOCUS       DAOOAB010000022        36340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1500_length_36340_cov_17.239658, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000022 DAOOAB010000000
VERSION     DAOOAB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 36340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36340
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_36340_cov_17.239658"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1069
                     /locus_tag="PKU88_06545"
     CDS             119..1069
                     /locus_tag="PKU88_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPX68980.1"
                     /translation="MKKNVLGNTGIEVTELCFGALPMGPLQKNLPDEESAEIVAYALE
                     SGITFVDTAQLYRTYLPIKSAIERTGIRPVIATKSTAASYEEMEKAIKEALDGMGIDY
                     IDIFHLHAAKVDTDVFEIRKGALQCLLDYKSRGIIKAVGISTHNVKVVEAAAARKDID
                     IVFPLLNKAGRGILEGTEEEMRGAISLCGQNGKGIYLMKVLGGGTLIDEYKDCVEYGR
                     RLDYCHSIAVGMVSREEVMYNVKYFNGEKDLDDIIKIKKKKSIKIAQTVCTSCGKCIE
                     VCHSGAPGFDHTGKAYIDQSKCIQCGYCIPSCRFFAIRMV"
     gene            1091..1732
                     /locus_tag="PKU88_06550"
     CDS             1091..1732
                     /locus_tag="PKU88_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPX68981.1"
                     /translation="MDKNVKFVVDERIKRTVENLEKNNISAYRVENEEEALKKVGELI
                     PEGSTIGIGGSMTLFEVGAIDYIRKGNYNVLDRYKEGLTPSEMKEILRKGFFADAYLT
                     SSNAVTENGELYNVDANGNRVASMLYGPDRVIVIVGINKLVKDIEEAVLRVEETAAPA
                     NNKRLNKTNPCTKTGHCMDCNSEGRICNEYTIIRRQGVKGRMHVIIVNKELGY"
     gene            1830..2663
                     /locus_tag="PKU88_06555"
     CDS             1830..2663
                     /locus_tag="PKU88_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX68982.1"
                     /translation="MQITVLVDNNTIIDRYLLGEPGVSFFIRADGKNILFDAGYSDIF
                     ITNAGKLGISLYDVDYAVISHGHIDHTWGLEPMIRMYSEAMIEKIGFRKPVLIAHPQA
                     FESKYHDNEVIGSVISEKALGDFFDMNLKREPFWITDRLVFLGEIERTNSFENKKPIG
                     KRAVQGNEEPDFLRDDTALAYKSEKGLVIITGCSHSGICNIIEYAKKVCNDDRIADVI
                     GGLHLLSPEEEVLELTKKYIKQAGVKKLHACHCTDLKSKLALSEAAELEEVGVGLVLE
                     F"
     gene            complement(2802..3281)
                     /locus_tag="PKU88_06560"
     CDS             complement(2802..3281)
                     /locus_tag="PKU88_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HPX68983.1"
                     /translation="MHYCQKDKIYSTKFLRDYFHGDSLKVPYDIDYDKSIWIKEMCDK
                     CLTGILHCLDRESRLAYLFRDVAQLSCEEIAEIMEQNPAAIRKTVSRSRKKLRNFLNN
                     ECVLFNPSGSCKCRMKNLVEDIMLPREYQELKRFVSRVNLYKESQSILPQKNYWISN"
     gene            complement(3332..3691)
                     /locus_tag="PKU88_06565"
     CDS             complement(3332..3691)
                     /locus_tag="PKU88_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX68984.1"
                     /translation="MEFGERLKDLREEKNLTRHLLASILNVSYSTVSKYETNIRFPDK
                     DTLVALADFFDVSLDYLLCRSDIKDTADKALSKCKQQFMVTSSGNIYNPYEGLSPEAV
                     EEIEKFKEFVQHKYGKK"
     gene            3876..4115
                     /locus_tag="PKU88_06570"
     CDS             3876..4115
                     /locus_tag="PKU88_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68985.1"
                     /translation="MDFGQNTVIYNACEIARKLFTVNEKDEKSNGRVVRKCFMCDISS
                     CFDMGNICCKECSIKNCRYKCNFADKENCEHKYIE"
     gene            4256..5422
                     /locus_tag="PKU88_06575"
     CDS             4256..5422
                     /locus_tag="PKU88_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPX68986.1"
                     /translation="MEKGRRLFSIIKAILFTLLFCTAIYFENAQRQRLYALVFIFALY
                     LAVGFGRGLIRRYDNLYFLSFILDIALVYTLEYNSRLLINYFFHSFYIAVLLESSLLL
                     GLRRGIMIGAATVVVSLIKYIYLIYYNFNLSNVSQLVFFLMVNVLILVVAGFAQHNKE
                     ERERKDILYRELLDAHKKLKQYTDEVNRLSVVDERNRIARDIHDTLGHNMTALIMQLQ
                     MAEHLLKEDPKKSEDLLVSSVKTAKDSMASIREVVETLRGTGTALAPVEFIRKLVNDF
                     SIKTGVEIKLEISDEANIQDSAANIAVYHIVQEAMTNSVRHGKAERISIRLDYSNESI
                     TFCIVDNGIGAETLKEGYGLKGIRERVEAFNGKFETGSSDGFYLKGIVYLEGKK"
     gene            5419..6066
                     /locus_tag="PKU88_06580"
     CDS             5419..6066
                     /locus_tag="PKU88_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68987.1"
                     /translation="MIKVLLVDDQDILVEGLKLILGREEDIQIIGTANNGGKAYEACR
                     GLRPDVVLMDIKMPEVSGVEATGMIKKDFPDIKIIVLTTFNDDEYIYDALKSGASGYM
                     LKDASPSEIAGAVRTVYNGGALIQSSVAVKVLDKFSELARGDRSKHKDSRAELLTDRE
                     KEICRLIAEGKNNREIADELYLSEGTVKNHITRVLIKLDLRDRTQLAVFTIKNDL"
     gene            6309..7307
                     /locus_tag="PKU88_06585"
     CDS             6309..7307
                     /locus_tag="PKU88_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX68988.1"
                     /translation="MKKLMIAILALMLAAALTSCSFDSLEGYKKAAEKTEQIKKGRTE
                     GEFSLVMDFNTTGMTEEQIKELYYFKNMKGSFNVAYDDELEKGIFRYYLIFGGLGFDL
                     DVFVNGEEIFMKLPVVGKYTRLNEMEKHMDDKQGEGEPEVISEETQDAIKEKWLGLLK
                     REDVFRGKDMVLTTPDGEVKANEYTIRLNDEQIKSLAENCIDILSKDKKLEENYGKYI
                     RKNVEFLKDTSLEKLLSDIKENIKKYTVESFGYTAYVDIDGYIVNEKVGITLEVDNAE
                     PASVTKVSYELDIKNWDINKDQEFDFPALTDENTLEMENIEQNMPFKIEALSNIKD"
     gene            7327..8256
                     /locus_tag="PKU88_06590"
     CDS             7327..8256
                     /locus_tag="PKU88_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX68989.1"
                     /translation="MLKVVNLHKSFGGTKAVDGISFEVDSGEVFGLLGPNGAGKSTTI
                     SIISTLILPTEGEVYYKGANILKEPNSVRRNLGVVPQDIALYPTLTGYENLRFWGNMY
                     GLKGAELKKRINEVSEIIGLNGRLKDRVDKYSGGMKRRLNIGAALLHKPELLIMDEPT
                     AGIDPQSRNHILDTVLELNRQGVTIIYTSHYMEEVEYLCSRICIMDQGRIIASGTQEE
                     LVDLVKGETRINLRLDRTDKDILQRLEAINGVAGAELVQDEIVVSGENVDMILADIIG
                     SIAEHGMRISSIDVSKPNLEAVFLHLTGKALRD"
     gene            8268..9653
                     /locus_tag="PKU88_06595"
     CDS             8268..9653
                     /locus_tag="PKU88_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68990.1"
                     /translation="MKVISIIKKDLKTILSDKKALAIIIVMPLLLMTILGSALRGAFT
                     TGDDQSIARINIAVVKQYDKDGDSRRFDKALRSGFLVKGIGEEAAAELRVTGEDVDPE
                     RIFLEDFLGSEDVAKIIAYRVEKEETAVEWLKSGEVSAVVLLPENFIYDMKINLLTPF
                     RNKTDIRVLTHPDRNVSGQIVRSIVEAYSDSMTSVIIGKNVLIEAAIRHNLMEDSYKG
                     MKEALDDITEAMEGIRVKIDAVEAEGRKPIKSSEYYAAAMAAMFILFAAGNGGRMLLE
                     EKENITYQRMIIAGTPKMGILAGKFFTVFLIALLQISIMIIFSRFVLKVQWGSAYPVV
                     LISLCAAFAIAGIGSMLAAATYRAGNYKMANIFETVILQTMALLGGSFFPIDILPSIM
                     QKLSFLSISGAVLKAYLKIMMGYGVQEIMSNIAALAAIGFLTAVLSVLILRENGGMRD
                     AQYNKAKTAGA"
     gene            9616..10785
                     /locus_tag="PKU88_06600"
     CDS             9616..10785
                     /locus_tag="PKU88_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX68991.1"
                     /translation="MLSIIKLRLLGLRDDFAVIVLMTVMALGFTAIFGISFDDFKPTV
                     LVVDEDKSSYSEALINELKGDRGLYFEVSNMKDAAAEVEEGKAIAALVIPKGFDEGIK
                     SGERASLGVLKIKDDILIFTMQKVVSGIVLKMAEGERIADITADFIHTLKPEAGQAVK
                     VFAYRSVMEAWEYKRPLEVTSSVAGTGADSSYDNLKHYMIGFSLFFSMYTMVFGIGTI
                     LNDRQYKTWQRMLITPVSKASILGGNMVTAYFIGAIQIGILILGGKYLLGIDWGSSIS
                     GILTVAGSFVFAVTSMGLMLSGVVKTQTQLSAVTPVVLTSTSMLGGCMWSLDIVNNKV
                     LLFLAELTPQKWAIQGMESIVSKGMGFEAAVYPAIVLMIMGVLFFAVGVKIMRFE"
     gene            10859..10935
                     /locus_tag="PKU88_06605"
     tRNA            10859..10935
                     /locus_tag="PKU88_06605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10893..10895,aa:Val,seq:cac)
     gene            11074..12756
                     /locus_tag="PKU88_06610"
     CDS             11074..12756
                     /locus_tag="PKU88_06610"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPX68992.1"
                     /translation="MEIKVPSDIEIAQNSQMKTVSQIAEQWGIEDDELELYGKYKAKI
                     NLKIFERLKDKPDGKLVLVTAITPTAAGEGKSTTTVGLGQALNKIGKKAIIALREPSL
                     GPVFGVKGGAAGGGYSQVVPMEDINLHFTGDMHAITAANNLLAAAIDNHINHGNTLNI
                     DPRQIVWKRVMDMNDRALREIVVGLGGKANGFVRQDGFMITVASEIMAILCLSNDLMD
                     LKERFGRMVVAYNYSGEPVTCSDLRVHGAMAILMKDAIKPNLVQTLENTPALIHGGPF
                     ANIAHGCNSIIATKLGLKLGEYLITEAGFGADLGAEKFFDIKCRYGGLKPSAVVIVAT
                     IRALKMHGGVAKAELGKENPEAVDKGFENLEKQVENIRKFGLPVLVAVNRFVTDTEAE
                     IELLVKKCKVYGVEVALNECWSKGGEGGTYMAETLVKLVEGSAPDFKYLYNVEAPIAE
                     KIETIAKEIYGGDGVVFTPKAQREMQKFADIGLDKLPVCMAKTQYSLSDDPTLLGVPK
                     GFKVSVKEVRVSAGAGFIVCLTGDIMIMPGLPKIPAAEKIDIDENGVITGLF"
     gene            12890..13786
                     /gene="ftcD"
                     /locus_tag="PKU88_06615"
     CDS             12890..13786
                     /gene="ftcD"
                     /locus_tag="PKU88_06615"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPX68993.1"
                     /translation="MNRIVECVPNFSEGRDLGKIEKIVNPFRGKDNVKLLDYKRDEDH
                     NRVVVTVVGEPQAVKDSILEAMGIAIELIDMTKHRGQHPRMGAVDVVPFIPIKNVSME
                     EAVELAKEVAGEASEKYGLPIFLYEKAAADPQRENLANIRKGEFEGMAEKIKLPEWKP
                     DYGPAEIHPTAGAAVIGARMPLVAFNVNLNTNRMDIANKIARNVRFLNGGLRYCKAIG
                     IELKDRGMVQISMNMTDYTKTALYRAFELIRIEAARYGVTIAGSEIIGLLPMEALIDT
                     AVYYLGVEDFSMDQILENRLME"
     gene            complement(13846..14337)
                     /locus_tag="PKU88_06620"
     CDS             complement(13846..14337)
                     /locus_tag="PKU88_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPX68994.1"
                     /translation="MTENEKQVYEVLNQLKIPYTQYEHVPVYTIQQLDELNMDMKGKH
                     CKNLFVRNDKGDKHYLIIVSQEKRADLKDIAKQIGSSRLGFASEERLMKHLGLTPGAV
                     TPFGLLNDEEKKVTVLVDEDLKNSIHICFHPNVNTATVSLLFDDFIKFLQWKGNEYQF
                     IKI"
     gene            complement(14491..14784)
                     /locus_tag="PKU88_06625"
     CDS             complement(14491..14784)
                     /locus_tag="PKU88_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HPX68995.1"
                     /translation="MYDELHRHNFEGRTGRKASHFHNYSGVTTKNPDFEGHVHYMSGY
                     TTEENEHIHYYSILTSPDFQVDGGHVHFFQCITTMDSGHYHLIYGYTSVYTEY"
     gene            14929..15609
                     /locus_tag="PKU88_06630"
     CDS             14929..15609
                     /locus_tag="PKU88_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX68996.1"
                     /translation="MKAKILVVDDDFNIADLIRLYLEKEMYEVKTVYSGRTALDVFRS
                     WTPDLVILDIMLPETDGYEVCRQIRKISGIPIIMLTARGETFDKVLGLELGADDYLVK
                     PFEPKELTARVKAVLRRTMGSDTIVEEVVYPQLVINKSDYSITFQGRKADIPPKELEL
                     FYFLASHPNQVFTREQLLGKLWGYDFMGDSRTVDVHVKRLREKFSDEAGRWEIKTVWG
                     VGYKFEVR"
     gene            15609..17018
                     /locus_tag="PKU88_06635"
     CDS             15609..17018
                     /locus_tag="PKU88_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX68997.1"
                     /translation="MKNSIQKRLFITYGVAIIIGFAILALSLLRIFDQYFIENRKELL
                     YEQGRKVAREAATVLYSGNPNSRSLINDLQVLDRFLDAHIWVINSDGIIVAVSGLNEE
                     RLLGRQVREEKIGNLAKGESIEERGNFDGMLGESSLTVGYPVFVNNRFSGGVLVHASL
                     LEIQENFREIYRLTLWVILISVAIAYAILYIQIRRISEPLKEISGAAKVIAGGEFHKR
                     LNINTGDEIEELGNSFNNMAKSLERIEENRRNLIANISHDLRSPMTSIRGFIEGMIDG
                     TIPEDKREHYLNIVLDESNRLIKMTNELLELGNMQQGSLEIKKEAFELNETIRRKLVA
                     YEKGITEKKLDVVFSMFEEKTFVLSDQVLLERVLSNLMDNAVKFTPEKGSIEIRTSTK
                     EDRIKVEITNTGKSISSEELNRVWERFHKGDTSRGLHKGGYGLGLAIVREIISQLGER
                     IWVSSGEGYVKFTLTIEKA"
     gene            17104..18273
                     /locus_tag="PKU88_06640"
     CDS             17104..18273
                     /locus_tag="PKU88_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPX68998.1"
                     /translation="MSEFFEENNENNEMNNTFYREVIERKPEKSSRKFIALVLALGLG
                     LGTIFGYGIDNFLPSPGSNANNVYAAAVDNTKIVYSGTVSPVVPIAEKVSPSIVAISL
                     KTRTRDFFGRIFEGQGTGSGIIIDKQGHIVTNNHVVEGADDIIVILHDGKELEATLIG
                     RDSQTDLAVIKVDSDNLTVAELGDSSALKVGELAVAIGSPMGTEYAGSVTAGIISGLN
                     RTVSIGDDSIKLIQTDAAINPGNSGGALVNSEGKVIGINTIKFAETRVEGMGFAIPIN
                     EAKPIIEDLINNKKVLRPFLGIQGITISKEEAEEYKLIQGVYVRLVIPGSGAEAAGVR
                     RGDIITKIDDKKVLTIEDLIAEIQKHKVGDTVKIEVYNQANEYKTLTVKLTASND"
     gene            complement(18309..19229)
                     /locus_tag="PKU88_06645"
     CDS             complement(18309..19229)
                     /locus_tag="PKU88_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX68999.1"
                     /translation="MKKYEAYLPYLAGFTVALIFGLSFMFTKQALETLPTALLLSSRF
                     SLAALLLTFLWLSGAIKINLKNKPLKKLMLLSLFQPIAYFIFETTGVKLTSSSEAGIM
                     IALIPVVVSIFAAVFLKERPSKSQLFFIAASVLGVIFIVLMSGSEDSTGHFAGILFLF
                     GAVLTAGVYNILSRKFSADFTPVEITFAMMWAGAAVFSIVSAVTGMINGNLDDYITYL
                     SSASTLIPILYLGILSSVCAFFMMNYMLSKMPASNSSVFSNLTTVVSIIAGVFIRHEA
                     FHWYQFAGSVVIILGVWGTNRCKPRHEIQL"
     gene            19442..19801
                     /locus_tag="PKU88_06650"
     CDS             19442..19801
                     /locus_tag="PKU88_06650"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPX69000.1"
                     /translation="MGLWGKDIARTKYVGNVYVDQISSIKKENKALFRIASKCENCNN
                     NDYYSIYETFRLFRILSIPLVQCDTVYYFSCPECNFGFKLEDEEFKKLEKIALINSKY
                     MEGSITKSELETGLKDI"
     gene            complement(19832..20188)
                     /locus_tag="PKU88_06655"
     CDS             complement(19832..20188)
                     /locus_tag="PKU88_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69001.1"
                     /translation="MKKSLWIALALIIGILPGFFLVFNFMFSDIISLNERILSFLIVI
                     AAYLILGAAFGLAGSDIGWKWGIWISLPAVIIALAYSFSEAGTLAINLAYSASALGSS
                     AISAYLTSKFLRKRKQ"
     gene            20380..20645
                     /gene="ffs"
                     /locus_tag="PKU88_06660"
     ncRNA           20380..20645
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKU88_06660"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            20823..23147
                     /locus_tag="PKU88_06665"
     CDS             20823..23147
                     /locus_tag="PKU88_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX69002.1"
                     /translation="MNRINFRLRTLLYFLIVSIIPFAVSWAFIYDSYYKKMQQDFRSF
                     NHMYIQNQFSKIGQQMYQHEMVLKSVAQAYSFLDPQTADLNAFLRDQNSVNLIFRNLS
                     IIMPDNSVYTGNPGYLKPDINYTLLFSYINARKAQELVWLESYKETLSGVQCVGMSIP
                     LLDNNNKASGVLVGNISLWTFENLLSNAKYLPYAEVFLVNPAGYVKYHSGNKYSEIVN
                     VTDKDFFLSPAADSILKSDEGYEEFSYAGKNWLCSFSSINSAGWKVLSLIDAQMLQST
                     FGVMNQNINRSIMSSGILCILIGIAASFFMSNSITTPLIKLRDGVKATTEGNLNNRII
                     VKGDNEIREVADAFNEMASNLNKTYTDLVKRTEELYANNEELHNVNIELEASYGQLGA
                     TVAQLNESDAQLRRKNSELQTLNKISNALNSTMDLSTMLITVVNQVTDITESLACTIR
                     LINEKDPYKLELKAMKGINTEMYDIGTIDIREDVIGRAVKTGSIYIIDLDNEKVPHEY
                     FMRLNKEHDAKCIVFTPIMVKSKVIGVLSITLKQIPSEELIELLNSLTNSIAIALDNA
                     KAYESLKQSYHKTVRSLVSVVEAKDQYTESHSIRVAKYASFIASELNYPKNFIEDIWV
                     AGLLHDIGKIGISDSILNKPGALTKEEYDIIKQHPEIAYKIVSKIGLRENVLKAIKHH
                     HERCDGKGYPDMIKSGEIPIMASIISVADAFDAITSNRPYRKSRSMKQGIDEIVMHRG
                     TQFDSTVAETLKKVFLTKHEILERIYNDEEIEFF"
     gene            23243..24859
                     /gene="dnaX"
                     /locus_tag="PKU88_06670"
     CDS             23243..24859
                     /gene="dnaX"
                     /locus_tag="PKU88_06670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPX69003.1"
                     /translation="MYTALYRKWRPQRFEDICGQEHITVTLKNELESGRLSHAYLFCG
                     TRGTGKTTTAKLLAKAVNCKASHGIDPCNECESCVSINDGNSIDVYEIDAASNRGIDD
                     IRNLREAIRFTPTLGEYKVYIIDEVHMLTNEAFNALLKTLEEPPEYVLFILATTEPHK
                     LPATILSRCQRFDFKRISSEKIVERLKFVCREEGLEAEEDAVKLIARNSDGAMRDALS
                     ILDQCAVYGSKKITHDDVLEVLGIVNNQYLFRIAEAVLEEDAGTAIKVIDEVAAGGKD
                     ISQFIRDLLVHYRNLLMAKVVTNCEDVIDMSKEGIEQLKKQSGSYDKNNIVRCINCLS
                     ELENEAKWSVNPRILLEVALVKMCRVGEEKSLEGILTRLSKLEERISKGSFDLKAADK
                     PKAAGQEEKREAAVKKEKAAAESKETAVSNPDSGSIIEKWPGFIQEMKVRGKIRLRTY
                     LAMAKPVKAESGIMVLSFEKEGVFSKEALELPANRADVEEAASKYFGAPLKIKCIISG
                     EEEDEQDSIVKAAIEFAGMDKVEVVEEEEE"
     gene            24863..25204
                     /locus_tag="PKU88_06675"
     CDS             24863..25204
                     /locus_tag="PKU88_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPX69004.1"
                     /translation="MGKGGFPGMGGNMNNMMKQVQKMQREMAKLQEEIEGRSVEASAG
                     GGAVTVVANGKKEILSITIKPEVIDPDDAEMLQDLITAAVNEAIRQADEMISREMSKL
                     TGGLNLPGGLF"
     gene            25204..25803
                     /gene="recR"
                     /locus_tag="PKU88_06680"
     CDS             25204..25803
                     /gene="recR"
                     /locus_tag="PKU88_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPX69005.1"
                     /translation="MSFYAAPIAKLIDELSKLPGVGNKTAQRLAFHILNMSSEDVEKL
                     SSSIVSAKRNIRYCKVCCNITDSDLCDICANAKRDSSTICVVEDPKDVVAMEKTREFK
                     GLYHVLNGAISPMEGIGPEEIRIKELLQRVADNEIKEIILATNPNIEGEATAMYISRL
                     LKPIGIKVTRIAHGVPVGGDLEYADEVTLMKALEGRREI"
     gene            25892..26182
                     /locus_tag="PKU88_06685"
     CDS             25892..26182
                     /locus_tag="PKU88_06685"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HPX69006.1"
                     /translation="MSTNLADREPKKNKQIEDIIDCLLNSMMGTRERDENAELINTIL
                     IAKQEMHDAQNYFDNVTAPELVDHAIFRMEAAKSQYVYLLKLAKDKGLSVTI"
     gene            26284..26553
                     /locus_tag="PKU88_06690"
     CDS             26284..26553
                     /locus_tag="PKU88_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HPX69007.1"
                     /translation="MPQIDVFSILAVFFAIGAVYVIGVLLVLPIKIIVKLVMNGIIGA
                     VALFAINIFSGLTGLSIGINPITALVAGFLGIPGIILLLFLQYIL"
     gene            26794..30321
                     /gene="nifJ"
                     /locus_tag="PKU88_06695"
     CDS             26794..30321
                     /gene="nifJ"
                     /locus_tag="PKU88_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPX69008.1"
                     /translation="MAKKMKTMDGNAAAAHVAYAFTEVAAIYPITPSSTMAENVDEWS
                     AHGLKNIFGQQVKVVELQSEGGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMYKIAG
                     ELLPGVFHVSARALASHALSIFGDHQDVMATRQTGFALLASGSVQEIMDLGGIAHLAA
                     IKSRVPFVHFFDGFRTSSEVQKVEVMDYADLEKLLDKKALDEFRKNALNPEHPVTRGT
                     AQNPDIYFQAREACNKYYDAVPDIVADYMKEISKLTGREYKPFNYYGAPDAENIIVAM
                     GSVTDAVEETVDYLMAKGEKVGVVKVHLYRPFSEKYFFDVLPKTVKKIAVLDRTKEVG
                     STGEPLYLDVRSMFYNKKNAPLIVGGRFGLGSKDTTPSQIKAVFDNLKTNEPKDGFTI
                     GIVDDVTNLSLEVKENINAAPEGTIRCKFWGLGSDGTVGANKSAIKIIGDKTDLYAQG
                     YFAYDSKKSGGVTISHLRFGKKPIKSPYLIDEADFIACHNQAYVHQYDVIKGLKDGGT
                     FVLNCKWNADELEENLPANMKKYIADHKIKFYIINATDIAVKIGLGNRINMIMQSAFF
                     KLANVIPIEDAVKYLKESLAETYGKKGQKIIEMNYKAVDAGTDALVKVTVPESWSSAK
                     EEAAAAVDVPDFIKNVLIPMNRQEGDTLPVSKFVEYADGHLPMGTAAYEKRGIAVFVP
                     EWQKEKCIQCNQCSFVCPHAAIRPVLVAEDELKKAPAGFETIKASGKGFEGVSYRMQV
                     SPLDCTGCGNCADICPAKEKALVMKPLDTQTEKGIDNWNFAMTVPVKDNLMTKSTIKG
                     SQFAQPYLQFSGACAGCGETPYIKVVTQLFGERMMIANATGCSSIWGASTPSTPYCKD
                     ANGRGPSWGNSLFEDNAEYGFGMAVAVNQIRAKLAEIMTEALALNMPNEYKEAYKAWL
                     DGKDDADASKAAAEKILPLLYNMSALSGRAKEIAAEIADKKDQLVKKSIWILGGDGWA
                     YDIGYGGLDHVLASGENVNVMVFDTEVYSNTGGQSSKATPVGAVAKFTASGKKTKKKD
                     LGMIAATYGYVYVAQVGMGADKNHFLKVIQEAEKYDGPSLIICYAPCINHGLKEGMGR
                     TQANTAQAVEAGYWHLWRYNPELKKEGKNPFILDSKEPKASFRDFLMNQVRYSSLVGA
                     FPEEAEKLFAKAEKEAKEKYENYKKMAEA"
     gene            30442..31020
                     /locus_tag="PKU88_06700"
     CDS             30442..31020
                     /locus_tag="PKU88_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPX69009.1"
                     /translation="MGKLIEIRWHGRGGQGAKTAALLLADAAFNTGKYIQGFPEYGPE
                     RMGAPITAYNRISDERIKIHSNIYEPDYVVVVDESLISAIDVTSGLKETGAIIINTDK
                     KPDEVKKHLKGYKGKVCTIDARKISEETIGRNFPNTPMLGAVVVVGNIMEPQKFLEDM
                     KESFAHKFKTKPEIIEGNMKAIERAMQEVQEL"
     gene            31017..31331
                     /locus_tag="PKU88_06705"
     CDS             31017..31331
                     /locus_tag="PKU88_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX69010.1"
                     /translation="MRGKAKEMTPEVTWQEITPGGTIYETGNAEGYKTGDWRSMRPIW
                     IEEKCTQCMLCPPVCPDTAIPVKDSKRLDFDYDHCKGCGVCFKVCPFDAIDFIKEEDK
                     EE"
     gene            31347..32528
                     /gene="porA"
                     /locus_tag="PKU88_06710"
     CDS             31347..32528
                     /gene="porA"
                     /locus_tag="PKU88_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPX69011.1"
                     /translation="MSIRERLSGNEAIAVAMRQINPDVVAAFPITPSTEIPQYFSQFV
                     SNGQVQTEFVAVESEHSAMSACIGAQAAGARTMTATSANGLALMWEMLYIASAMKLPI
                     TLSCVNRALSGPLNIHNDHSDSMGARDSGWIQLYSETNQEAYDNLLQAIRIAEHKDVL
                     LPVMVCQDGFITSHAVENIELLEDDKVKEFVGEYKPDHYLLNHKEPIAVGPLDLPAHC
                     FEHKREQAQSMINAKKVILEAAKEFEKLTGRKYEYFEKYNLDDAEIAIVIINSSAGTA
                     KTVADKLRDNGIKAGLLKIRMFRPFPADEIAEALKNVKALAVMDKAESYSAMGGPVGA
                     EVKSALYGKADGIKVLNYIYGLGGRDVRADDIEEVYNSLMYIVKTGQIGETYRYIGVR
                     G"
     gene            32545..33483
                     /locus_tag="PKU88_06715"
     CDS             32545..33483
                     /locus_tag="PKU88_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX69012.1"
                     /translation="MAYNLKQVMNKPERLTGGHRMCAGCGAPVTVRTVLRALKPEDHA
                     VCCSATGCLEVSTFLYPYTAWKDSFIHSAFENAGATLSGAEAAYTALRRKGRMNESYK
                     FIAFGGDGGTYDIGLQSLSGAMERGHDMVYICYDNEAYMNTGIQRSSGTPRYADTTTS
                     PQGTEMPGKMQYKKDLTGVMAAHRIPYVAQSTLTSNFKDLYEKSEKAIYTKGPAFMNV
                     LSPCPRGWRYDTPDLMEICKLAVETCIWPLYEVEDGKWKLNYKPKKKLPVIEYLKPQG
                     RFKHLFTDENKHLIDNIQEDVDRKWEELLKKCGEDS"
     gene            33660..35669
                     /locus_tag="PKU88_06720"
     CDS             33660..35669
                     /locus_tag="PKU88_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX69013.1"
                     /translation="MRLSFKITVPIVILLIAAIASLSGVSFYFTGKLIESNMSQLSGS
                     KLEEVQNIILQRNAEVSISRMELKMEFLNKAEILANVIKQNPGFIQNNSALFDMTASL
                     EVDEIHITDENGIIRWGTIATSYGLDLNYNERMKPFLPALTDSNFEFVQDPIVRGSDN
                     ALFQYAGVARKDKPGIILIGVSGLKLQSELEKADISGISKDTSFGANGIVIIVDKESD
                     KIISHKDMAIQGGKAAEYDWGRKIRESDSGEFKYISDGTEYFTKYQDTGQYIVCTVIP
                     VSEFTSGMSLFLRNIVMISAAALVLCILIIYILLKINVTNEINKLLKSLKTIGEGDLT
                     KTIDIRSSKEFSKLSEGINVMSNNLREIIEKGLEMTKSLSESGKRLAESADISSKGAS
                     EIATTINELAEGATEQADGATKGALTAKDVLSKAEAISLNIEDTVKNTELTKDTVLAG
                     VEIIKYQNEKMKESVLSAEDLGSSINDLSKRADEIGDITEVITGIANQTNMLALNAAI
                     EAARAGEIGKGFAVVADEVRKLAEGATKSALKISEIIAEIQSGIGNAKIQADKSIRAI
                     EEQQTAVKQTEEAFIKINNVTQEAVNQVGRIAEATENIISGIHKIVDIVESQAAVSQE
                     SAAGTEEITASMQEQTAAIEEVAQIANDLAEVVGELNALISKFKI"
     gene            35816..35962
                     /locus_tag="PKU88_06725"
     CDS             35816..35962
                     /locus_tag="PKU88_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69014.1"
                     /translation="MSIVCEKVIEQFGQDKKLINLIIYNGESIIYNLNDEELNAEELP
                     VLTN"
     assembly_gap    36172..36271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT        11730 a         6348 c         8954 g         9208 t
ORIGIN      
        1 gggagagcgc tgcgttcgca acgcagaggt cgggggttcg aaccccctat cgtccaccat
       61 ttaaaagtta agattagtga ttgaatatat ttaagtaaca atgtaaggag atgactacat
      121 gaaaaagaac gttttaggaa atacaggaat tgaagttact gaattatgct ttggagcact
      181 gcctatggga cccctccaaa agaaccttcc ggatgaggaa tctgctgaaa ttgttgcata
      241 tgccctggaa agcgggataa catttgtgga tacggcacag ttgtacagaa catatttgcc
      301 gataaaatca gctattgaaa gaacggggat cagaccggta attgcaacaa agtctacagc
      361 tgcatcttat gaagaaatgg aaaaagccat aaaagaggca ctggatggaa tgggtataga
      421 ctatattgac atttttcatc ttcatgcagc aaaagtcgac accgatgttt ttgaaatacg
      481 gaaaggtgct ttgcagtgcc tgctagacta taaaagcaga gggataatta aggcagtggg
      541 gatatcgacg cacaatgtaa aagtagtaga agccgcagca gcaagaaaag atattgatat
      601 tgtttttccg cttcttaaca aagcaggccg ggggatattg gaaggaacag aggaagaaat
      661 gaggggcgcc atatccctgt gcgggcaaaa cgggaagggc atatatctga tgaaagtatt
      721 agggggcggt actttaatag atgagtacaa agactgcgtg gaatatggca gaaggttgga
      781 ttactgccat tctatagctg tcggaatggt aagcagggaa gaggtcatgt acaatgtaaa
      841 atattttaac ggggaaaaag atttggatga catcataaaa atcaagaaaa agaaaagcat
      901 aaaaattgca cagacggtat gcaccagctg cggcaagtgc attgaagtat gccatagcgg
      961 tgcccccggc ttcgatcata cgggcaaagc atacattgat cagtcaaaat gtatacaatg
     1021 cgggtattgt attccgtcct gccggttctt tgctatcagg atggtgtgat aagaaaggag
     1081 aagatgtata atggataaaa acgtaaaatt tgtagttgat gagaggataa aaaggactgt
     1141 agaaaatctg gaaaagaaca atatctcggc ttatagggtt gaaaacgaag aggaagcatt
     1201 gaaaaaggtt ggcgagctaa taccggaagg aagtacgata ggcatcggcg gctctatgac
     1261 gctttttgag gtgggtgcta ttgattatat aagaaagggc aattataacg ttttggacag
     1321 gtacaaggaa ggcttgaccc cgtcggaaat gaaggaaatc ctcaggaagg gattttttgc
     1381 agatgcctat ttgacaagca gcaatgctgt taccgaaaac ggagaactct ataatgtgga
     1441 cgcgaacggc aacagggttg cttctatgct gtatgggcct gaccgggtta tcgttattgt
     1501 agggataaac aaacttgtca aggatattga agaagcggtt ttacgagtcg aagaaacagc
     1561 ggctcctgcc aataataaac ggctgaataa aaccaatcca tgtacaaaaa cgggacattg
     1621 catggattgt aattccgaag gaagaatctg caatgaatat acgataatca gaagacaggg
     1681 tgtaaaggga agaatgcatg taattattgt gaataaagaa ctggggtatt aaagaatttg
     1741 gaccttcctg ttgttaataa caggaaggtt ttttattata gaagcaatgg aattttagta
     1801 aatggataaa tgaatgggag gctggattta tgcaaatcac agttctggtt gacaacaata
     1861 cgataattga ccgttatctg ctgggggaac ccggtgtgtc cttttttatc cgtgcggatg
     1921 ggaaaaacat tctgttcgat gcgggttact cggacatatt tatcaccaat gccggaaagc
     1981 ttggaatcag cctgtatgat gtggattatg cggtgatatc ccatgggcat attgaccaca
     2041 cctgggggct tgagcccatg ataagaatgt attcggaggc gatgattgaa aaaatcggct
     2101 tcaggaagcc tgttcttatt gcccatccgc aggcttttga aagcaagtat catgataatg
     2161 aagtaatagg ttccgtaatc tctgaaaagg cgctgggaga tttttttgat atgaatttga
     2221 aaagggagcc cttttggatc acggataggc tggtattcct gggcgaaata gaaaggacaa
     2281 acagctttga gaataaaaag cctataggga agcgtgcggt tcaaggcaat gaagaaccgg
     2341 atttcctgag ggatgatacc gccttggctt ataaatcgga gaaggggttg gttattatta
     2401 ccggatgctc ccattcaggc atatgcaata taatagaata tgcaaaaaaa gtctgcaatg
     2461 acgacaggat tgcggatgtt ataggagggc ttcacctatt gagccctgaa gaagaagtcc
     2521 tggaactgac aaaaaaatat atcaaacagg caggtgtaaa aaagcttcat gcctgccatt
     2581 gcaccgacct gaaatcaaag ctggcattat cggaggcggc ggagcttgaa gaagtaggag
     2641 taggattggt attggaattt tgaaaaaact tgcgagcctt gtaactcgca agcttttttc
     2701 ttttgttcag ttaccttttc aagtacgccc ccgttttttt aatacccgtt aaaggttttc
     2761 attagtatta gagaaggtca gggtgaaatt gtgacacatt tttaattact gatccaataa
     2821 tttttttgag gcagtatact ttgggactcc ttgtagagat tgactctgga tacaaaacgc
     2881 tttaattcct gatactcccg gggcagcata atgtcttcca caagattctt catgcggcat
     2941 ttacagctgc ctgaaggatt gaacagaaca cattcgttgt tcagaaaatt cctgagcttt
     3001 tttctgctgc gggatactgt tttccttatt gccgcagggt tctgttccat aatctcggca
     3061 atctcttcgc aggaaagctg tgcaacatcc ctgaacaaat atgccaaacg gctttcacgg
     3121 tcgaggcaat gaagtatgcc tgtcaggcac ttgtcacaca tttcttttat ccatatagat
     3181 ttgtcatagt caatatcata gggtacctta agggaatccc cgtgaaaata gtcgcgcagg
     3241 aattttgtag aataaatctt atccttttgg cagtaatgca ttactgagtg ataggttact
     3301 gtgtatatcc atgtagacag tgtagcttcg cctacttctt tccatactta tgctgtacga
     3361 attccttgaa cttctcaatt tcctcaacag cctccggaga taagccctca tagggattat
     3421 atatgttccc cgaagaggta accatgaatt gctgtttgca cttgctgagt gccttgtctg
     3481 cggtatcctt aatatcactt ctgcagagca aatagtccag ggacacatca aaaaaatccg
     3541 ccaatgccac taatgtatcc ttatccggaa atcttatatt agtttcatac tttgatactg
     3601 tcgaataaga aacattcaat atactagcga gcaaatgcct tgtcaaattt ttttcttctc
     3661 tcagatcttt taatctttca ccaaattcca tgggaataca cctcaaaata ttgcattaat
     3721 ttttgcggtt ttatagtatg atgttaacac aattcgtcca attcggacta aaaatggaca
     3781 aataggggca aaaattgttg acagtggaca tgtagtccag taagataata ttgttgttgg
     3841 actatatgtc tattattatt cggggaggta tttgcttgga ctttggacag aataccgtga
     3901 tatataatgc ttgcgagatt gcaagaaaat tatttacagt taatgaaaag gacgaaaagt
     3961 ccaatggacg tgttgtgaga aaatgcttca tgtgtgacat atcaagctgc tttgatatgg
     4021 gtaacatttg ctgtaaagaa tgcagcataa agaactgccg ctataaatgc aatttcgctg
     4081 ataaagaaaa ctgtgagcat aagtatattg aataatgagc atggggatat gcggataacc
     4141 taaaaaatgc tgatgatttg agggcatact gcgcatataa taataaccca tggatataat
     4201 gcatctgata ttgtataata ttttcatgaa ttcaaaacaa tggaagggta tcggtatgga
     4261 aaaagggaga aggctcttta gcattattaa agccatatta tttaccttgt tgttctgtac
     4321 tgcaatatat tttgaaaatg cgcagcggca gaggctatat gcgcttgtat tcatatttgc
     4381 actgtacctg gcggttggct tcggaagagg cttgattagg cgttacgaca atttgtactt
     4441 cctgtccttt atccttgata tagcattggt atatactctt gaatacaatt caagactcct
     4501 gataaactac tttttccatt ccttttatat agcggtattg ttggagtcat ccttgctgct
     4561 gggcctcaga aggggtataa tgatcggggc tgcaacagtc gtggtatctc tgataaaata
     4621 catatatttg atttactata atttcaacct ttcaaacgta tcccagctgg tttttttcct
     4681 tatggtcaat gttcttatac tggttgttgc gggttttgca cagcacaaca aggaagaaag
     4741 ggagcggaag gatatactct acagggagct tctggatgcg cataaaaagc tgaaacagta
     4801 taccgatgaa gtgaaccggc tttcggtggt tgatgaaaga aaccgcatag caagggatat
     4861 acatgatacc ttgggccata atatgactgc ccttatcatg cagctgcaga tggctgagca
     4921 tcttttgaag gaagatccca aaaagtcgga ggatctgctg gtaagctcgg taaaaaccgc
     4981 caaggacagc atggcaagca tcagagaggt ggttgagact ctgaggggaa ccggtactgc
     5041 ccttgcaccg gttgaattca taaggaagct cgtaaatgat ttttcaataa aaaccggtgt
     5101 ggagataaag cttgaaattt ccgatgaggc taacattcag gattcggcgg cgaacatagc
     5161 ggtttaccat attgtccagg aagcaatgac taactctgta aggcatggca aagcagaaag
     5221 gatatcgatc cggcttgatt attccaatga atccataaca ttttgcatag ttgataatgg
     5281 cataggggca gagactttaa aggaaggcta cggtttgaag ggaatcaggg aacgggtgga
     5341 ggctttcaac ggaaagtttg aaacaggaag cagcgacgga ttctatttaa aaggtatcgt
     5401 atacttggag ggtaagaaat gattaaggtg ctgttggttg atgatcagga catcctggtg
     5461 gaaggcctca agcttatact tggaagagaa gaggatattc agataatcgg gactgccaat
     5521 aacggcggaa aggcttacga agcatgcagg ggcctccgcc ccgatgtggt gcttatggat
     5581 atcaagatgc cggaagtaag cggtgtggaa gctaccggta tgataaaaaa ggattttccg
     5641 gatataaaga taatagttct tactaccttc aatgacgatg agtacatata tgatgcgctc
     5701 aaaagcggcg cttcaggcta tatgcttaag gatgcctcac cctcggagat tgccggggcg
     5761 gtgcgtacgg tatataacgg cggcgctctt atacagtcgt cggtggcggt aaaggtgctg
     5821 gacaaattct cagagcttgc ccggggagac cggtccaagc ataaggattc cagggcggag
     5881 cttctgacag atcgtgaaaa agagatatgc aggctgattg cggaaggaaa gaacaatagg
     5941 gaaattgctg atgagctata cttaagcgaa ggcacagtta agaaccacat cacaagagtg
     6001 cttattaagc tggacctgcg ggacaggaca cagctggcgg tatttactat aaagaatgat
     6061 ctttaaaggc ggcattcccg ccttttctta cgtatctgaa gaatgatgcg tcggggggac
     6121 atggggacgt ttcgcgtgtc ccgctgaaag caggacaggg gaaacgtccc catgtccccc
     6181 cgacgcatta tccatatatt gtgcaaaaag tcatggggta tgctgcttaa taagtgattt
     6241 aagatacaag aaatgctgct tttgaatctg tataatggta tcgtaagata attttggagg
     6301 gattttcagt gaaaaaatta atgattgcga tattggcttt aatgctggca gcggcattaa
     6361 ccagctgcag cttcgacagc cttgaaggct ataagaaagc ggcggaaaaa acagagcaaa
     6421 taaaaaaagg caggacagaa ggggaattct ccctggtgat ggatttcaac acaaccggaa
     6481 tgacagaaga gcagataaag gagctgtact attttaaaaa tatgaaggga agcttcaatg
     6541 ttgcttatga cgatgaactt gaaaagggaa ttttcagata ctatttgatt tttggcggct
     6601 taggctttga tctggatgtc ttcgtaaacg gagaagaaat attcatgaag ctgccggtcg
     6661 ttgggaagta tacaaggtta aacgaaatgg agaagcacat ggacgataaa cagggggaag
     6721 gggaaccgga ggttatttcc gaggaaacac aggacgcgat taaagaaaag tggcttgggc
     6781 ttcttaagag ggaggatgta ttcaggggta aggatatggt actcaccact cccgacgggg
     6841 aggtaaaggc taatgagtac accataagat tgaatgatga gcagattaaa agcctggcgg
     6901 aaaactgcat agatatactt tccaaggaca aaaagcttga agagaactac gggaaataca
     6961 tcaggaagaa tgtggagttc ctgaaggata cgtctttgga aaaactgctt tcggacatta
     7021 aggagaatat taagaagtat actgttgaaa gcttcggata cacggcctat gtggatattg
     7081 acggatatat agtaaatgaa aaggttggaa tcacattaga ggttgataat gcggaaccgg
     7141 cgtcagtaac caaggtcagc tatgaattgg atataaaaaa ctgggatata aacaaggatc
     7201 aggaatttga ctttcctgca ctgacagatg aaaatactct ggaaatggaa aatatcgaac
     7261 agaacatgcc ctttaagatt gaggcactgt ccaatataaa ggattaagag gggtaacgat
     7321 atggcgttgt taaaggtggt aaatctgcat aaaagctttg gcggtacaaa ggcagtggac
     7381 ggcataagct ttgaggtaga cagtggagaa gtgttcggac tgcttggccc caacggggca
     7441 ggaaagtcca ctaccatctc cattatatct acattaatac ttcctacaga gggggaggta
     7501 tattataagg gggcaaacat attaaaggag ccgaattccg tcagaaggaa tctgggtgtg
     7561 gtaccccagg atatagcgtt gtatcctaca ctaacgggct atgagaatct gcgtttttgg
     7621 gggaacatgt acgggcttaa aggagcagag ctgaaaaaaa ggatcaatga ggtttctgaa
     7681 ataatcggcc ttaacggccg cctgaaggat agggtggata agtattcggg aggaatgaaa
     7741 agaaggctga atatcggagc ggcactgctg cacaagccgg aactgcttat tatggatgaa
     7801 ccgacggcag gcatagatcc ccagtccagg aatcatatat tagataccgt cctggagctt
     7861 aacaggcagg gggttacaat catatacacc agccactaca tggaagaggt ggagtatctg
     7921 tgcagccgta tctgcataat ggatcaggga aggataatag cctccggaac ccaggaagaa
     7981 ctagtggacc ttgtaaaagg agaaaccaga ataaatctca ggcttgacag gacagacaag
     8041 gatatacttc aaaggcttga agccataaat ggggtagccg gggcagagtt ggtgcaggat
     8101 gaaattgtcg tttcagggga aaatgttgat atgatattgg cagatattat cggcagcata
     8161 gctgagcatg gaatgcgcat ttcatccatt gacgtcagca agccgaatct ggaagccgtc
     8221 ttcctgcact tgacaggaaa ggccctgagg gattaaggag ataaaacatg aaagtaataa
     8281 gtatcataaa gaaggattta aaaacgatac taagcgacaa aaaggctttg gcaatcataa
     8341 ttgtgatgcc tctcctgctt atgacgatat tgggctctgc tttaagaggg gcatttacaa
     8401 caggagatga ccagagtatt gccaggataa atattgcggt ggtgaagcag tatgataagg
     8461 atggggattc ccggaggttt gacaaggctt taagaagcgg ctttcttgta aagggaatag
     8521 gagaggaggc tgctgcggag ctgagagtga ccggtgagga cgtagacccg gagcggatat
     8581 ttcttgagga tttcctgggc agtgaggatg tggcaaaaat tatagcatac agagtagaga
     8641 aggaggaaac agccgttgaa tggcttaaaa gcggggaggt ttctgcggtg gtgcttcttc
     8701 ccgagaattt catatacgat atgaagataa atcttctgac acccttcaga aacaaaactg
     8761 atataagggt gctgacacat ccggacagga acgttagcgg tcaaatagta cggagtattg
     8821 tggaagctta ttccgattca atgacttcgg ttataatagg gaaaaatgtt ctgatcgaag
     8881 cggctataag gcataatctt atggaagaca gctataaggg aatgaaggag gctttggacg
     8941 acatcactga agcaatggag ggaataaggg taaaaattga tgctgtggaa gcagagggaa
     9001 ggaagcctat taaaagctca gaatactatg ctgcagcaat ggcggcaatg ttcatacttt
     9061 ttgcagccgg taacggaggg agaatgcttc tggaggagaa ggagaatata acttaccaga
     9121 ggatgataat agcaggaaca ccaaaaatgg gaatactggc aggaaaattc tttactgttt
     9181 tccttattgc cctgcttcag ataagtataa tgatcatatt ctcacgcttt gtgctgaagg
     9241 tccagtgggg aagcgcgtat ccggttgttt taatcagctt atgtgctgct tttgccatag
     9301 cgggaatcgg aagcatgctt gcagcagcca catacagggc aggcaattac aaaatggcga
     9361 acatatttga aacagttatc ttgcaaacta tggcactttt gggaggcagc ttcttcccga
     9421 tagacatact gccttcaata atgcagaagc tgagcttttt atccatcagc ggggcagttt
     9481 tgaaagcata tcttaagatt atgatgggat atggggtaca ggagattatg agcaatattg
     9541 cagcattggc ggcaataggg tttttaactg cagtgctttc ggttttgata ctcagggaga
     9601 acggagggat gagggatgct cagtataata aagctaagac tgctggggct tagggacgat
     9661 tttgcggtga ttgttctgat gaccgtcatg gcccttggct tcactgccat tttcggcatc
     9721 tcttttgatg acttcaagcc gacggtactg gttgtggatg aggataaaag cagttattcc
     9781 gaagccttga tcaatgaact gaagggcgat aggggattat actttgaagt ctcgaatatg
     9841 aaggatgcgg cggcggaagt ggaagaaggc aaggcaattg ccgcactggt tataccaaag
     9901 ggctttgatg agggcataaa aagcggcgaa agagcttctt tgggggtatt aaagatcaag
     9961 gacgacattc tcatattcac aatgcagaag gtagtatcgg gtatagtcct taaaatggcg
    10021 gagggtgaaa gaatagcaga tatcactgcg gattttatac acacccttaa gcccgaagcc
    10081 ggccaggctg tgaaggtatt cgcatatcga agcgttatgg aggcatggga atacaaaagg
    10141 cctttggaag taacctcttc tgttgccggt acaggagcag acagcagcta cgacaatctt
    10201 aaacattaca tgataggctt ttccttgttt ttctcgatgt atacaatggt gttcggcata
    10261 ggaacaatat taaatgacag acagtataag acatggcagc gaatgctgat aacccccgta
    10321 tccaaagcct caatacttgg gggaaacatg gtgacagcat atttcatcgg tgccattcaa
    10381 ataggaatat tgatacttgg agggaaatat ctgctgggaa ttgactgggg aagcagtatt
    10441 tcaggaattc tcaccgtggc gggttctttt gtatttgccg ttacttccat gggacttatg
    10501 ctgtccggtg tggtaaaaac ccaaactcag ctttctgcgg ttaccccggt ggtgcttacc
    10561 agtacttcaa tgctgggagg ctgcatgtgg tctttggata tagtaaacaa taaagtgctg
    10621 cttttcctgg cggaactcac acctcagaaa tgggcaatac agggtatgga aagtatagta
    10681 tccaagggga tgggctttga agcggcagtg tatcctgcaa ttgtgcttat gataatggga
    10741 gtgttgttct ttgcagtcgg agtgaaaatt atgaggtttg aatagattag caaataataa
    10801 aaaatactat ggataatgtt aacatgtaga gttataatat atatattgat tttcgagtgg
    10861 gggattaact cagccggtta gagtgccatc gtcacaaggt ggaagtcgca ggttcgaatc
    10921 ccgtatcccc caccattgct aaaaaatcat agagctatcg aaagatcgct ctatttttat
    10981 agaggtgttt ggataccctt tttggatgga tttgcattct ctcttagatg catttacata
    11041 attctgacat tattttacga aaggaatgat taaatggaaa tcaaagtacc cagcgatatt
    11101 gaaatagcgc agaactcaca gatgaagacc gtatcgcaga ttgcggagca atggggaata
    11161 gaagatgatg aactggagct atacggcaag tataaggcaa aaatcaatct aaaaattttt
    11221 gagcgattaa aagacaaacc tgacggaaaa ctggtattgg ttactgctat cactcccact
    11281 gctgcaggag aaggcaagtc gacaaccacg gtaggcttgg gccaggctct taataaaatt
    11341 gggaaaaagg cgataatagc tttaagagaa ccgtctttgg gcccggtttt cggcgtgaag
    11401 ggcggagccg cagggggagg atactcccag gtagtgccta tggaggatat caatcttcat
    11461 tttaccggag acatgcatgc aattactgca gccaacaacc tgcttgctgc agccattgac
    11521 aatcatataa accacggaaa cacactgaat atagatccaa ggcagatagt ctggaaaaga
    11581 gtaatggata tgaacgacag ggcactccgt gaaatagtgg tgggccttgg aggaaaggcc
    11641 aacggctttg taaggcagga cggttttatg ataactgtgg cttctgaaat aatggctata
    11701 ctgtgccttt ccaatgattt gatggattta aaagaaagat tcggccggat ggttgttgca
    11761 tataattatt cgggagaacc ggttacctgt tccgatttaa gggttcatgg ggctatggcc
    11821 attcttatga aggacgccat aaagcctaac ttggttcaga ctttggaaaa cacacctgca
    11881 cttattcacg gcggaccctt tgccaacata gcccacggct gcaacagcat aattgctaca
    11941 aagctgggct tgaagctggg ggaatacctt attaccgagg caggcttcgg ggctgatctt
    12001 ggagcagaga agttctttga catcaaatgc cgttatgggg gcttaaagcc ttccgcagtt
    12061 gtaatagtag caaccatacg tgctcttaag atgcacggag gggttgcaaa ggcggaactg
    12121 gggaaagaaa acccggaagc agtggataaa ggatttgaaa accttgaaaa gcaggtggaa
    12181 aacatccgca agttcggact tccggtattg gttgccgtta acaggtttgt aaccgatacc
    12241 gaagccgaaa tcgaactgct tgtcaaaaag tgcaaggtat atggcgtaga ggttgcattg
    12301 aacgagtgct ggtcaaaagg cggagaaggc ggaacatata tggccgaaac ccttgtaaaa
    12361 ctggttgaag gctcagcacc tgattttaaa tatctttaca atgttgaagc tcctatagcc
    12421 gaaaaaatcg aaactattgc aaaggaaatc tatggcgggg acggtgttgt tttcactccc
    12481 aaagcacaaa gggagatgca gaagtttgcg gatatcggac tggataagct gcctgtctgt
    12541 atggcaaaga ctcaatactc gctgtctgat gaccccactc ttttaggtgt tccaaagggc
    12601 ttcaaggtta gtgttaaaga agtgagggta tctgccggag cgggctttat agtatgtctt
    12661 acgggggaca ttatgataat gccgggactt ccaaagatac ctgcagcaga aaagattgac
    12721 atagatgaaa atggcgtaat aacaggttta ttttagtgac gaaacccgta atatttgcag
    12781 caaattaaat gctggaaata tagggattta gtcatataag tgtaaaactt ttatgtgaaa
    12841 cataaaagtt cagacactag accataaagg ggggaaagca atgagcggga tgaacagaat
    12901 agttgaatgt gtcccgaatt tcagtgaagg aagagatttg ggcaaaatag agaaaatagt
    12961 gaatcccttt aggggaaagg acaacgtaaa gcttctggat tataagagag acgaagatca
    13021 caacagagtg gtcgtaactg ttgtgggtga acctcaggca gtgaaggatt ctatactgga
    13081 agctatgggt atcgcaatag agcttataga tatgaccaag cataggggac aacaccccag
    13141 aatgggggca gtagacgtag tacccttcat acccataaag aatgtaagca tggaagaggc
    13201 cgttgaactt gcaaaggaag tggcaggaga ggcatcggag aagtatgggc ttccgatttt
    13261 tctgtatgaa aaggctgcag cagatcccca aagagaaaac ctggcgaata ttcgcaaggg
    13321 agaattcgaa ggtatggcgg agaagataaa gttgcctgag tggaaaccgg attacggccc
    13381 ggcagaaatt catcctacag caggagccgc ggttatcggc gcaaggatgc ccctggtagc
    13441 ctttaatgta aatttgaata ctaacagaat ggatattgcc aataaaattg caaggaatgt
    13501 caggttttta aacggcggtt tgcggtactg caaggctata ggcattgagc ttaaggacag
    13561 gggaatggtg cagatttcca tgaatatgac tgattataca aagaccgctc tttacagagc
    13621 ctttgagctt atcagaatag aagcggccag atacggcgtt acaattgccg gcagtgaaat
    13681 aataggactt ctgcctatgg aagccttgat tgatacggcg gtatattacc tgggcgtgga
    13741 ggatttctcc atggatcaga tactggaaaa caggttaatg gagtagaaga ataatgcggc
    13801 gcgaggacat gtccccatgt ccccgtgtcc ctccgtcccc atgcctcaaa tttttatgaa
    13861 ttggtattcg ttgcctttcc actgcaagaa ctttatgaag tcgtcaaaaa gcagcgatac
    13921 agttgctgta tttacattgg gatggaagca tatatgtatt gaattcttaa ggtcttcatc
    13981 cacaagtaca gttacctttt tttcctcatc attcagcagg ccgaagggag tgactgcacc
    14041 gggagtcagc ccaaggtgct tcatcagtct ttcttccgaa gcaaagccta agcgggagct
    14101 gccaatctgt ttggcaatgt ctttaaggtc tgccctcttt tcctgggaga caataataag
    14161 gtaatgcttg tcgcccttgt catttctgac gaagagattc ttgcagtgct tacctttcat
    14221 atccatattt aattcatcaa gctgttgaat tgtatatacc ggtacgtgtt catactgtgt
    14281 atatggaatt ttcagttgat tcagcacttc gtatacttgt ttttcatttt ctgtcattgc
    14341 agttgtatgc ctccttgact tctgtgtata attatattac cacaaaagca agaggcataa
    14401 aagtgttcgt aatccttaac ttagaagcct aaccctaaat ttcgcgtctc gcgtctcgaa
    14461 tctcgtgtct gaaaattgcc gtgcaatttt ctaatattcc gtataaacac tggtataacc
    14521 gtatataaga tgataatggc cgctgtccat agttgttatg cattgaaaga aatgtacatg
    14581 tccgccatcc acctgaaaat ccggactggt taatattgaa tagtagtgaa tatgttcatt
    14641 ttcctcggtt gtataaccgg acatgtagtg tacatggcct tcgaagtcgg gatttttggt
    14701 tgtcactccg gagtaattat ggaaatgcga ggctttccgg ccggtcctgc cctcaaaatt
    14761 gtgcctgtgc agctcatcat acatattgca taccccctgc tataaatcta tgcagcggca
    14821 agggcttttg acatctattt ttctatgcat ttgaattatc acagacattg atgtatcata
    14881 atggataaaa cgaggcaaaa agatgttcaa taggcggtgg taaaagatat gaaagccaaa
    14941 atactggttg ttgatgacga cttcaatata gcggacctta taaggctgta cctggagaag
    15001 gagatgtacg aggtaaagac ggtatactcc ggcaggactg ctcttgatgt attccgttca
    15061 tggactccgg atcttgtcat tcttgatatt atgcttccgg aaacagacgg ctatgaggta
    15121 tgcaggcaaa taaggaagat aagcggtatt cccattatta tgcttaccgc aaggggagaa
    15181 acctttgaca aggtactggg cctggaactg ggagcggatg attatcttgt aaagcctttt
    15241 gaaccgaagg agcttactgc aagagtaaaa gcagtgctca gaagaaccat gggctctgat
    15301 acaatagttg aagaagttgt atatcctcag ctggtaatta acaaaagcga ttacagcatt
    15361 acattccaag gcaggaaggc ggacatacca ccgaaggaac tggagctgtt ctatttcctt
    15421 gcaagccatc caaatcaggt ttttacgagg gaacagctgc tgggaaaact atggggatac
    15481 gatttcatgg gtgactcgag gactgtggat gttcatgtaa aaaggctcag ggagaagttc
    15541 agtgatgaag cgggcagatg ggaaataaag actgtttggg gcgtgggcta taaatttgag
    15601 gtgagatgat gaagaacagc atacagaaga gattgttcat aacctatggc gtagcaataa
    15661 taataggctt tgctatactg gccttgtcac ttctcaggat attcgaccag tatttcatcg
    15721 aaaacaggaa agagcttctg tatgagcagg gcaggaaggt ggccagggaa gctgcaacag
    15781 tgctttattc ggggaatccc aattccagga gtcttattaa tgatttgcag gtcctggaca
    15841 ggtttttgga tgctcatatc tgggttataa acagtgatgg aataatagtt gccgtttccg
    15901 ggctcaacga agaaaggctg ctgggcaggc aggttaggga agagaagatc ggaaaccttg
    15961 caaaagggga aagtattgag gagagaggta attttgacgg gatgcttggt gaatcttctt
    16021 tgaccgtagg ctatcccgta tttgtcaaca acaggttttc cggaggggtg ctggtacacg
    16081 cttcacttct tgagatacag gaaaatttca gagaaatata cagactgacg ctttgggtta
    16141 ttctgatttc cgttgccatt gcttatgcaa tcctgtatat ccagatacgg aggatttctg
    16201 aaccattaaa ggaaataagc ggagctgcaa aggtaattgc cggaggggag ttccacaagc
    16261 gcttgaatat taatacggga gatgaaattg aagaacttgg caacagcttc aacaatatgg
    16321 cgaagtcatt ggaaagaata gaagagaaca ggcggaattt gattgcgaac atatcccatg
    16381 acctgcgctc gccgatgact tcaatcaggg gtttcattga aggaatgatt gatggtacta
    16441 tacctgagga taaaagggag cattatctga acatagttct ggatgagagc aacagattga
    16501 taaagatgac caatgaactg ctggagcttg gcaatatgca gcaaggaagc cttgaaatca
    16561 agaaggaagc ctttgagctt aatgagacaa taagaaggaa gcttgttgca tatgaaaaag
    16621 gcataacaga gaaaaaactt gatgtagttt tcagcatgtt tgaagagaaa accttcgtat
    16681 tgtctgatca ggtactgctt gaaagggtgc tttccaatct tatggacaat gcagtaaaat
    16741 tcacaccgga aaagggcagc attgagataa ggacatcaac aaaggaagac aggataaaag
    16801 tggagattac caataccgga aaatcgataa gctctgagga gcttaacaga gtatgggaaa
    16861 ggttccacaa aggggatact tcaagaggcc ttcacaaagg aggatacgga ttagggcttg
    16921 ccatcgtcag ggaaataatc agccagttgg gtgagaggat atgggtctcc agcggcgaag
    16981 gttatgtaaa atttacatta actattgaaa aagcttaaag tttacaattt gttcatattt
    17041 tgttcaaaac tttggattat atttatagta tagttcaagt acatccgtta ggagggataa
    17101 gatatgagcg aattttttga agaaaataat gaaaacaacg aaatgaacaa taccttttac
    17161 agggaagtta ttgaaaggaa gccggagaaa agctccagaa aattcatagc tttggtgctt
    17221 gctttagggc ttgggctcgg cacgattttc ggatacggga tagacaattt tctgcctagt
    17281 cccggaagta atgcaaacaa tgtatatgca gcagccgtgg ataataccaa aattgtatac
    17341 agcgggacgg tatcgcctgt agtgcccatt gcggaaaaag tttcaccgtc aatagttgct
    17401 atcagcctta agacgagaac cagagacttt ttcggcagga tttttgaagg ccagggaaca
    17461 ggctcgggaa tcataatcga caagcagggc catatagtaa ccaacaacca tgtagttgaa
    17521 ggggctgacg atataatagt aatactccat gacggcaaag agcttgaagc aactctgata
    17581 gggcgcgatt cccagacaga cctggcagta ataaaagttg actcggataa tctgacggtt
    17641 gcggagcttg gagactcttc agcacttaag gtgggagaat tggcagttgc cataggcagc
    17701 ccgatgggca ccgaatatgc cggctcggtt acagcgggaa taataagcgg actcaacagg
    17761 acggtcagca taggagatga ttccataaag cttatccaga ctgatgccgc aataaatccc
    17821 ggaaacagcg gaggggcatt ggtaaacagc gaaggaaagg tcatagggat taacacaata
    17881 aagtttgcag aaacaagagt tgaggggatg ggttttgcca ttccgataaa cgaggcaaag
    17941 cctattattg aggacctgat aaacaacaag aaggtattaa ggccatttct tggaatacag
    18001 ggaataacca taagcaagga agaggctgaa gaatacaagt tgattcaagg tgtttatgta
    18061 aggctagtaa taccgggcag cggtgcagaa gcggccggag tcagaagagg cgatatcata
    18121 acaaaaatag atgataagaa ggttctcaca attgaagatc tgatagcaga gatacaaaag
    18181 cataaggtgg gagacacagt aaaaatagaa gtgtacaatc aggcaaatga atacaagacg
    18241 cttactgtta agctgactgc cagcaatgat tagataagta agagacaggc aactgtctct
    18301 tatttgtttt aaagctgtat ttcatgccgg ggcttgcagc ggtttgtgcc ccagaccccg
    18361 aggataataa caacacttcc tgcaaactga taccaatgaa aggcctcatg cctgataaaa
    18421 acgccggcaa ttattgatac tacagtggta agattagaga ataccgaaga gtttgaagca
    18481 ggcatcttcg aaagcatata gttcatcata aaaaaagcgc acacagaaga gagaattccc
    18541 agataaagaa tgggaatcaa cgtgcttgcc gagcttaagt aagtaatata atcatcaaga
    18601 ttgccgttaa tcattcctgt taccgctgat acaatactga atactgctgc ccctgcccac
    18661 atcattgcaa aggtaatttc tacaggagta aagtcggcag aaaactttcg tgaaagaata
    18721 ttatatactc cggcggtcag tactgctccg aagagaaaga gtattccggc aaaatgacct
    18781 gtgctgtctt cgctgccgga catcaaaact ataaatatca cacctaagac ggaagcggct
    18841 ataaaaaaca gctgggattt gctcggcctt tccttgagga atactgcagc aaaaattgat
    18901 acaactacgg gtatcagagc aatcataatt cctgcttccg atgaggatgt aagctttaca
    18961 ccggttgttt caaaaataaa atatgcgatc ggctggaaca gagataacag cataagcttc
    19021 tttaagggct tgtttttaag gtttatctta atggcgcctg acagccatag aaaggtcagc
    19081 agtaacgcag ccaaagagaa acgggaggaa agaagaagag ctgtaggcag agtttccagg
    19141 gcttgttttg taaacataaa ggacaatcca aaaattaatg ctactgtaaa tcctgccaaa
    19201 taaggcaaat aagcttcata ttttttcacg ttatcacatc ctatatattt tgaggcagac
    19261 atttgatgaa gatgtcccga atatagttat tctagcagaa atgaaagggg cttgtctatt
    19321 taaagtttgg aaagcacggt tcattacaat tataggattt gcgggaagga ttttgaaatt
    19381 attgatagaa tatacaatac ataaatagtt tccggttgga tattgcgagg ggtgatatgc
    19441 tgtggggctt tgggggaagg atatagcaag gacaaaatac gtaggaaatg tttatgttga
    19501 ccagattagc agtattaaaa aagaaaacaa agcattgttc agaatagcgt ccaagtgtga
    19561 aaactgtaat aataacgatt attacagtat ttacgagacc ttcaggcttt tcaggattct
    19621 gagcataccc ttggtgcagt gtgataccgt ttattacttc agctgtcctg aatgcaactt
    19681 cgggtttaag cttgaggatg aggagttcaa gaagcttgaa aagatagcat tgattaattc
    19741 aaagtatatg gaaggctcaa taacaaaatc cgaattagaa acaggactga aagatattta
    19801 gaaacagggt caaaagcagt gacgcaatcc gtcactgctt tcgttttctt aaaaactttg
    19861 aggttaagta agcgcttata gcggaggaac caagcgcact tgccgaataa gcgaggttta
    19921 tcgccagcgt gcctgcctcg ctgaaggaat aggccaatgc aataataaca gcaggaaggc
    19981 tgatccatat tccccatttc cagcctatat cacttccggc cagcccgaag gccgccccca
    20041 ggatcagata agctgcaatt actatcagga aagagaggat tctttcattg agggatatta
    20101 tatcagaaaa cataaaatta aaaaccagaa aaaaccccgg cagaattccg ataataagtg
    20161 cgagagcaat ccataggctt tttttcaaaa tagcagctcc tttgcaaaag tatctatgat
    20221 aatagtgttg tctgtattca tattataata ccaatcatca attgtaaaat gcttcaataa
    20281 aagcctattt tgacgcattg acatcaaata tccgaaagct tataattaaa tagctaaata
    20341 tatcaaaata tattaatcac aaaaagttaa caatcttggc cgtgctagat ggggagactg
    20401 cggtgccctg taacctgcaa tccgcaacag cagggttgaa ttcctttgct gaggcgttcg
    20461 cctgtaagga ctgcccctgg taagtggtgt tgatgattgg gtcctgcgca atggaaattc
    20521 atgaaccccg tcaggtccgg aaggaagcag cggtaagtga accctttcat gtgccgcagg
    20581 gctgcctgat tggagctaac tgcccgggta acgcttgtag gtgcacgtcg agggaaggtg
    20641 cacggccata tactaaaaac ttgtgcaaac aagtttttta ttttacatta tatataggcc
    20701 ttcagaacta gaaccaagcc ttaggactta tttatgaaga ctattttgat attaattcct
    20761 ttacaggtat aataatacta acaaatgatg ccgaattatg tacagagggg ggattttttg
    20821 tcatgaacag aataaatttt aggcttagaa cgctactgta ttttttaata gtatctataa
    20881 tcccctttgc ggtgtcatgg gcttttattt atgattctta ttacaaaaaa atgcagcagg
    20941 atttcagaag cttcaaccac atgtacatac agaaccaatt cagtaaaatc ggacagcaga
    21001 tgtatcaaca tgaaatggtg ttaaaatcag ttgctcaggc atatagtttt ttggatcctc
    21061 agactgccga cctaaatgct tttctaagag atcagaacag tgtgaatcta attttcagga
    21121 acttatccat tattatgcct gataattcag tctatacagg caatccgggc tacttaaagc
    21181 ccgatatcaa ttatacgtta ctgttcagct atattaatgc aagaaaggcc caggaactgg
    21241 tgtggcttga atcctataaa gaaaccttaa gcggagttca atgcgtaggg atgtccatac
    21301 ctttgctgga taataataac aaagcttcag gggtattggt cggaaacata tctttatgga
    21361 cctttgaaaa tctgttatcc aacgcaaagt atctgcctta tgcagaggta tttcttgtca
    21421 atccggcagg ctatgtaaaa taccacagcg gaaataagta cagcgaaatt gtcaatgtaa
    21481 ccgataaaga cttttttctt agccctgcgg cagattccat acttaaatcg gatgaaggct
    21541 atgaagaatt cagttatgca ggcaaaaact ggctttgcag cttttcttct atcaattccg
    21601 ccggatggaa ggttctatcc ctcatagatg ctcaaatgct tcaaagcacc ttcggtgtta
    21661 tgaatcaaaa tatcaacaga tctataatgt cttcgggaat actttgtatt ctcataggaa
    21721 ttgcggcttc attttttatg tccaattcaa taacaactcc tcttataaag ctgagggatg
    21781 gggtcaaagc aactacagaa ggcaatttga ataacaggat tattgtaaaa ggtgataacg
    21841 aaataaggga agtagccgat gcttttaatg agatggcaag taacctcaat aaaacatata
    21901 ctgacctggt taagaggaca gaagaacttt acgccaacaa tgaagagctt cataatgtaa
    21961 atattgaatt ggaagcctct tacggacaat taggggcgac agtcgcacag cttaacgagt
    22021 cggatgcaca gctcagaagg aaaaacagtg aactgcaaac ccttaacaaa ataagcaatg
    22081 ccttgaattc cacaatggat ttgagcacca tgctcattac agttgttaac caggtcactg
    22141 acataaccga atcattggct tgcacaataa ggcttataaa tgaaaaggat ccatacaagc
    22201 tggagcttaa agctatgaaa ggaataaata ccgagatgta tgacataggt accattgata
    22261 taagggagga cgtaataggc agagcggtga aaaccggatc catatatata atagatcttg
    22321 ataatgagaa agttcctcac gaatatttta tgaggctgaa taaagaacat gatgccaaat
    22381 gtattgtttt tactccgatt atggttaaat ccaaggtaat tggcgtcttg agcattaccc
    22441 tgaagcagat tcccagcgag gaacttattg agttattaaa ttcattgaca aacagtattg
    22501 ccattgcctt agacaatgca aaagcttatg agtctctgaa gcagtcatac cacaaaactg
    22561 tgcggagcct ggtatcggtg gtcgaagcaa aggatcaata tacggagagt cattccataa
    22621 gagttgccaa gtatgcttcc tttattgctt ccgaattgaa ttacccaaag aattttattg
    22681 aagatatctg ggtggcgggg ctgcttcatg atattgggaa gatcggtatc agcgactcaa
    22741 tactgaataa gcccggagcc ctcactaaag aggaatacga tattatcaag cagcacccgg
    22801 aaatagccta taagatagta tccaaaatag gcctgagaga gaatgtctta aaagcaataa
    22861 agcatcacca tgaaagatgc gacggcaaag gttatcctga tatgataaag agcggcgaaa
    22921 taccgattat ggcttcgata ataagtgttg cagatgcctt tgatgcgata acctccaacc
    22981 ggccttatag aaaatcaagg agtatgaagc agggtataga tgaaatagtc atgcacagag
    23041 gtacacagtt tgactcaact gtggcagaga ctctgaaaaa agtatttctg acaaaacatg
    23101 agatcctgga gagaatatat aacgacgaag aaattgaatt cttctaatgc tcaggaatga
    23161 gcattttttt tatagttaat ttactatgac gctttattat gctaatattt tattgtacag
    23221 gaataacaca tggaggataa ctatgtatac ggctttatac agaaaatgga ggccccagag
    23281 gtttgaggat atatgcggcc aggaacatat aacagtgaca ttgaaaaatg agcttgagag
    23341 cggcaggctt tcccatgcat atttgttctg cggcaccaga ggcacgggca agaccactac
    23401 tgcaaagctt ttggccaagg cagtgaactg caaggcctct catgggattg atccttgcaa
    23461 tgaatgtgag agttgtgtca gtataaatga cggaaactca atagatgtat atgaaataga
    23521 tgccgcttca aacaggggaa tagacgacat aaggaatttg agggaagcaa taaggttcac
    23581 gcctaccttg ggcgaatata aggtatatat aatagatgaa gttcatatgc taacaaatga
    23641 ggcgttcaat gcacttctca aaaccctgga ggaaccgccg gaatatgtat tgttcatatt
    23701 ggcaacaact gaacctcata aactgccggc tacaattcta tcccgatgcc agaggtttga
    23761 ttttaaaaga ataagcagcg aaaaaatagt tgagcggctg aagtttgtct gcagagaaga
    23821 gggcttggaa gcggaagagg atgcagtaaa gcttattgca agaaacagcg acggggcaat
    23881 gagagatgca ttgagtatcc ttgaccaatg tgcggtttac ggcagcaaaa aaataaccca
    23941 cgatgatgta cttgaggtac tgggcattgt gaacaaccaa tatttgttca gaatagcgga
    24001 agcagtactt gaagaggacg ccgggacggc aataaaggtg attgatgagg tagcggcagg
    24061 agggaaggat ataagccagt ttatcagaga tttgctggta cattacagga atcttctgat
    24121 ggctaaagtt gtgacaaact gtgaagatgt gatagatatg tcaaaggagg gaattgagca
    24181 gcttaagaaa caatccggca gttatgataa aaacaatatc gtaagatgta tcaactgcct
    24241 ttccgagttg gaaaatgaag caaagtggtc ggtcaatcca aggatcttgc tggaagttgc
    24301 actggtaaaa atgtgccggg tcggagagga aaaaagcttg gagggtatac tcacaaggtt
    24361 gtcaaaactg gaggaacgta tttcgaaagg aagttttgac ctgaaggccg cagataagcc
    24421 aaaggcagca ggtcaggaag agaaaaggga agcggcagta aaaaaagaaa aagcagccgc
    24481 agaatcaaag gaaaccgcag tatcgaatcc ggacagcggg agcataatcg aaaaatggcc
    24541 cggttttatt caggaaatga aagtccgcgg gaaaatcagg ctcaggactt atctggcaat
    24601 ggcaaagccg gttaaagcgg agtcaggcat aatggtatta tcctttgaaa aagaaggggt
    24661 gttttccaaa gaagcccttg aactgcctgc aaacagggca gatgtggagg aagccgcatc
    24721 aaaatacttc ggggcacctc ttaagataaa gtgtattatt tccggtgagg aagaagatga
    24781 acaggacagc attgtaaaag ctgctattga atttgcaggc atggataaag ttgaagttgt
    24841 tgaagaagag gaggaataag aaatgggtaa aggcggattc cccggaatgg gagggaatat
    24901 gaataatatg atgaagcagg ttcagaaaat gcaaagagaa atggcaaagc ttcaggagga
    24961 gatagagggg agaagcgttg aagccagcgc cggcggaggg gccgtaaccg tagtagccaa
    25021 cggaaagaaa gagatactat caataacaat aaaacccgaa gttatagacc ctgatgatgc
    25081 agaaatgctc caggatctta tcactgcggc tgttaacgaa gctataagac aagcggatga
    25141 aatgataagc agagaaatga gcaagttaac cggagggctc aatttacccg gaggtttatt
    25201 ctaatgagtt tttatgcagc tcctatagcc aagcttatag atgagctttc gaagcttccc
    25261 ggagtaggca acaagacagc acagcggctg gcattccata tactcaacat gtcctccgaa
    25321 gatgtagaga agctttcttc ctctatagta agtgcaaaga gaaatatcag atattgcaag
    25381 gtgtgctgca atatcacgga ttctgatcta tgcgatatct gtgctaatgc aaaaagagac
    25441 agcagtacca tatgtgttgt ggaggatccc aaggacgttg tcgcaatgga gaagacaaga
    25501 gaattcaaag gcttgtatca tgtattgaac ggagcgatat cgcccatgga aggcataggg
    25561 ccggaggaga tcagaatcaa ggaacttctt cagagggtgg ctgacaatga aataaaggag
    25621 ataatactgg cgactaatcc gaatatagaa ggagaagcga cagctatgta tatttccagg
    25681 ctgttgaagc ctatcgggat caaggtaacc agaatagccc acggtgtacc tgtaggaggg
    25741 gaccttgaat atgcggatga agtgacattg atgaaggccc ttgaaggcag aagggaaata
    25801 taggctaaat ttatcttaaa atagagtaca cctcagaata ttattgctaa ttcttggata
    25861 aattagtaat aataacggag gtgttttatt tatgtccacg aatctggcag atagggagcc
    25921 taagaaaaat aaacagatag aggatattat agactgtttg ctgaatagca tgatgggcac
    25981 cagggaaagg gacgaaaatg cggagctgat aaatacgata cttatagcaa agcaggaaat
    26041 gcatgacgca caaaactatt ttgacaatgt aaccgcccct gagcttgtag atcatgcaat
    26101 attcagaatg gaagctgcca aatcacaata tgtttatctg ctgaagcttg caaaggacaa
    26161 ggggctcagc gtgacgatat agccggcctg tgataaaata gttgtacaaa acttgtaata
    26221 aaatacgaaa agagtaatat aaataaacat acgatcttta taacttaccg ggggggattc
    26281 aagttgccgc agatagatgt attctcaata ctggcagtat tttttgccat aggtgctgtc
    26341 tatgttatag gtgtattgct ggtcttgcct attaaaataa ttgtcaaact ggtaatgaac
    26401 ggtataatag gggcggttgc attatttgca atcaatattt tcagcggact cacaggcctc
    26461 tccataggga taaacccaat tacagcactg gtagcaggat ttttgggaat ccccgggata
    26521 atacttttat tgttcctgca atatattttg tagcaggttt gaaaaaactt gtcagatgaa
    26581 taataattga tagatgttca taattataaa attgaccaaa aaataactaa ctgcctttac
    26641 ccattgagag acatggatga gggtggttat tattttttaa gggttcaatg cttttaattt
    26701 gacaatgaaa tgaatgttta atatactaaa agtggcgttc tttgataaat taactacaaa
    26761 tgaccattta ttattatatt agggggttta gcaatggcta aaaagatgaa aacaatggat
    26821 ggtaatgccg cagctgcgca tgtcgcttat gcgttcacgg aagttgctgc aatttacccg
    26881 attacaccgt catccacaat ggctgaaaat gttgatgagt ggtccgccca tggactgaag
    26941 aacatttttg gccaacaggt aaaggttgtt gagctgcagt cagaaggagg agctgcagga
    27001 gcagttcacg gttcccttgc agcgggtgca ttaactacaa cctttacagc atcacaggga
    27061 ttgctcctta tgataccaaa tatgtacaag attgccggag aattgcttcc gggcgtattc
    27121 cacgtatctg cccgtgcatt ggcaagccac gcgctctcaa tatttggaga ccatcaggac
    27181 gtaatggcaa caagacagac aggatttgct ttacttgctt ccggttcagt acaggaaata
    27241 atggatctgg gcggtattgc tcacttggct gcaatcaaat caagagttcc gtttgtacac
    27301 ttctttgacg gtttcaggac ctccagcgag gttcagaagg tagaagtaat ggattatgca
    27361 gacctggaaa aacttcttga caagaaagca ttggatgaat tcaggaaaaa tgctttgaat
    27421 ccggagcacc cggtaacaag aggtactgct cagaatcctg atatatactt ccaggcaaga
    27481 gaagcttgca ataagtatta tgacgcagtt ccggacatag tagcagacta tatgaaggaa
    27541 ataagcaagc tgacaggcag agaatacaaa ccctttaact attacggggc gcctgacgct
    27601 gaaaacatca tagtagcaat gggttctgtg acggatgctg ttgaggaaac ggtagattat
    27661 ttaatggcaa aaggtgaaaa ggtaggagtt gtaaaggtac acctctacag gcctttctca
    27721 gaaaagtatt tcttcgatgt acttccaaaa acagtgaaga aaattgctgt acttgacagg
    27781 acaaaagaag tcggatcaac aggagaacca ttatatcttg acgttcgttc aatgttctat
    27841 aacaagaaaa atgctccatt aatagttgga ggaagattcg ggcttggttc caaagatact
    27901 acaccgtcac agattaaagc tgtttttgat aatttgaaga ctaacgagcc aaaagacgga
    27961 ttcacgatag gtatagtaga tgatgttaca aatctttccc ttgaagtaaa ggaaaatatc
    28021 aatgcagctc ccgaaggaac aataagatgc aagttctggg gtttgggttc cgacggtacg
    28081 gttggagcaa ataagagtgc aataaagatt ataggcgata agaccgatct ttatgcccag
    28141 ggatattttg cttatgactc caagaagtcc gggggtgtaa ccatttccca cctcagattc
    28201 ggcaagaagc ctataaaatc cccatatctt attgatgagg ctgatttcat agcatgccac
    28261 aaccaggcat atgttcatca gtatgatgta ataaagggac ttaaagacgg cggcactttc
    28321 gtattgaact gcaaatggaa tgcagacgag cttgaagaga atctgcctgc aaatatgaaa
    28381 aagtatatcg cagatcataa aatcaagttc tacataatca atgcgacaga tattgccgta
    28441 aaaataggcc ttggaaacag aatcaacatg ataatgcaat ctgcattctt caaactggcc
    28501 aatgttattc cgatagaaga tgctgtaaag taccttaagg agtctcttgc agaaacttac
    28561 ggcaagaaag gccagaagat tattgaaatg aactacaaag ctgttgatgc gggaaccgat
    28621 gcgcttgtaa aagttacagt tccggaaagc tggagcagtg caaaagaaga agcggcagcg
    28681 gcagtggatg ttcctgattt tataaagaat gtacttattc ctatgaacag gcaggaagga
    28741 gatacacttc cggtaagtaa atttgttgag tatgcagacg gacatttgcc aatgggaact
    28801 gctgcatacg aaaagagagg aatagcagta tttgtacctg agtggcagaa ggaaaaatgc
    28861 atacaatgta atcagtgctc ctttgtatgt cctcatgcgg caataagacc tgtgcttgta
    28921 gctgaagatg aattaaagaa agcacctgca ggatttgaaa caataaaagc ttcaggaaaa
    28981 ggttttgaag gagtatctta caggatgcag gtaagcccat tggattgcac aggctgcgga
    29041 aattgtgcag atatatgccc tgcaaaagaa aaagcacttg taatgaagcc ccttgatact
    29101 cagacagaaa aaggtattga taattggaac tttgcaatga ctgtacctgt taaggacaac
    29161 ttaatgacaa agtcaactat aaaaggcagc cagtttgcac agccatatct ccaattctca
    29221 ggagcatgcg caggctgcgg cgaaactcca tatatcaagg ttgtgactca gctctttggt
    29281 gaaagaatga tgatagcgaa tgcaacagga tgttcatcaa tctggggcgc ttcaacacct
    29341 tcaactccat actgcaagga cgcaaatgga agaggcccgt cctggggtaa ttccctcttt
    29401 gaagacaatg cagaatatgg atttggtatg gcggtagctg taaatcagat aagagcgaag
    29461 cttgccgaaa taatgacaga agcattggct ttgaatatgc ctaatgaata taaggaagca
    29521 tataaagcat ggctggatgg caaggatgat gcagatgcaa gcaaagcagc agcagaaaag
    29581 atacttcccc ttctttacaa tatgagtgca ttaagcggaa gagcaaaaga aatagcagct
    29641 gagatagctg acaagaaaga ccagcttgta aagaaatcca tctggatact cggtggtgac
    29701 ggatgggctt atgatatagg ctacggcgga cttgaccatg tactggcatc cggagaaaac
    29761 gtaaacgtaa tggtatttga tacggaagta tattccaaca ccggaggaca gtcctccaag
    29821 gcgacaccgg tcggagcagt tgccaagttt acagcatccg gtaagaagac aaagaagaag
    29881 gacctgggta tgatagcagc tacctacgga tatgtatatg tagctcaggt aggtatggga
    29941 gcagacaaga atcatttcct gaaggttatt caggaagcgg agaagtatga cggaccatct
    30001 ctcataatct gttatgcacc atgtatcaac cacggactta aggaaggcat ggggagaacc
    30061 caggccaaca ctgctcaggc tgtggaagca ggatactggc acctgtggag atataaccct
    30121 gaactcaaga aggaaggcaa gaatcctttc atacttgact caaaggagcc aaaggcaagc
    30181 ttcagagatt tcctgatgaa tcaggtaaga tattcatccc tggtgggtgc tttccctgaa
    30241 gaagcagaaa agctctttgc aaaagcagag aaggaagcaa aggaaaaata cgaaaactac
    30301 aagaagatgg cagaagctta aaaataaaaa tcagaaaaaa gtcgattgag aaatcaatcg
    30361 actttttatt ttcataaaaa aatcaaaata gcacagaata ccaatgtatt agaaagaaaa
    30421 gatatgaagg gaggaaaggt tttgggcaag ttaatagaaa tacgctggca tgggcgcggt
    30481 ggccaaggag cgaaaacagc cgcacttctc ctggcagatg ccgcttttaa cacaggaaag
    30541 tatattcagg gatttcccga gtatggccct gagaggatgg gagcaccgat aacagcctac
    30601 aacagaataa gcgacgaaag gataaaaatt cattccaaca tttatgagcc ggattatgta
    30661 gtagtggttg atgagtcttt gatttcggca attgatgtga cttccgggct taaagagacg
    30721 ggtgcaatta tcattaatac tgataagaaa cccgatgagg tgaagaaaca tctaaaagga
    30781 tacaagggca aggtttgcac catagacgcc aggaagatat ctgaagaaac cataggcagg
    30841 aattttccca atacacctat gcttggcgcg gtggtggtag tcggtaatat tatggagcct
    30901 cagaagtttc tggaggatat gaaggagtcc tttgctcata agtttaagac aaagcccgag
    30961 ataattgaag ggaacatgaa ggccattgaa cgggctatgc aggaggtgca agagctatga
    31021 gagggaaagc aaaagagatg actcccgaag tgacttggca ggagataacc ccgggaggca
    31081 ctatatatga gacgggtaat gcagaaggct ataagacagg ggactggaga tccatgaggc
    31141 caatctggat tgaagaaaaa tgcacccaat gcatgctgtg tccgccggtc tgtccggaca
    31201 ctgcaattcc tgtaaaggac agcaagaggc tcgattttga ttatgatcac tgcaagggct
    31261 gcggagtatg cttcaaggta tgtcctttcg atgcaataga ttttatcaaa gaagaagata
    31321 aagaagaata ggaggtgcag ttgcacatgt caataaggga aaggttatca ggaaatgaag
    31381 caatagcagt ggcaatgagg cagataaatc cggatgttgt tgctgcattc ccaataactc
    31441 cgtcaacgga gataccccag tatttctcac aatttgtatc taacgggcag gtgcagacag
    31501 agtttgttgc ggtagaatca gaacacagtg caatgtcagc ctgcattgga gcacaagcag
    31561 caggagcaag aacaatgact gccacttccg caaacggttt ggctttgatg tgggagatgt
    31621 tatatattgc ttcagcaatg aagctgccaa taactttatc ctgtgtcaac agagcactgt
    31681 cagggcccct gaacatacat aacgaccaca gtgattcaat gggagcaagg gattccggct
    31741 ggatacagct gtacagcgag accaaccagg aggcatatga caatctgctt caggctataa
    31801 ggattgctga gcacaaagat gttctgcttc cggtcatggt atgccaggac ggctttatta
    31861 ccagccatgc agtagagaat atagagcttc ttgaggatga caaggttaaa gaatttgtag
    31921 gagaatacaa accggatcat tatctattga accacaagga accaatagcc gtaggacccc
    31981 ttgaccttcc ggctcattgc ttcgagcata aaagagagca ggctcagagc atgatcaatg
    32041 caaaaaaagt aatactggag gcagcaaaag aatttgaaaa acttaccgga aggaaatacg
    32101 agtatttcga gaaatacaat cttgatgatg cagaaattgc tattgtaata atcaattctt
    32161 ctgccggaac agcaaaaaca gttgcagaca agttgagaga taatggtata aaggcaggac
    32221 ttctgaaaat aaggatgttc aggcccttcc cggctgatga aatcgcggaa gccttgaaaa
    32281 atgtaaaggc tctcgcagta atggataaag ccgaaagcta ttctgcaatg ggaggccctg
    32341 tcggtgctga agtaaaaagc gcactctacg ggaaagcgga tggaataaag gttttgaact
    32401 atatttatgg tttaggcggc agggatgtaa gggctgatga tatcgaggaa gtatataact
    32461 cccttatgta tatagtaaaa acaggtcaaa taggagaaac ttacagatat ataggtgtaa
    32521 gaggatagga ggcgggatat tttcatggca tataatctta agcaagtaat gaataagccg
    32581 gaaagattaa ccggagggca caggatgtgt gcaggctgcg gagcgcctgt tacggtgaga
    32641 acggtattaa gagcattgaa gcctgaagat catgccgtat gctgttcggc tacaggatgt
    32701 cttgaggtat ctacattcct gtatccttat acggcctgga aagattcatt tattcattca
    32761 gcatttgaaa acgcgggagc tacactgagc ggcgcggaag ctgcatatac tgctctaagg
    32821 agaaaaggca gaatgaacga atcttataag ttcattgcct ttggagggga cggagggact
    32881 tatgatattg ggcttcagtc actttccgga gcgatggaga gaggtcatga catggtgtat
    32941 atatgctatg acaatgaggc atatatgaac accggaattc aaaggtcatc aggaacacca
    33001 aggtatgcag atacgactac aagcccgcag ggtaccgaaa tgccggggaa aatgcagtac
    33061 aagaaggatc tgacaggagt aatggctgcc catagaatac cctatgtcgc tcaatcgaca
    33121 ttaacttcaa actttaagga tctttatgaa aaatcagaga aagccatata tactaagggc
    33181 ccggcattta tgaatgtact ttctccctgc cccaggggct ggaggtacga cacccctgat
    33241 cttatggaaa tatgcaagct ggcagttgaa acatgtatat ggccgttgta tgaagttgaa
    33301 gatgggaaat ggaagctcaa ctacaaaccc aaaaaaaagc ttccggttat tgaatatttg
    33361 aagccccagg gaagatttaa acacttattt acagatgaaa ataaacacct cattgataat
    33421 attcaagaag atgtcgacag aaaatgggag gaattattga agaagtgcgg agaagattcc
    33481 tgaaaaacgt gcaaaagcac gtttttttat attgggaata acagtatata taaatgactt
    33541 tctgtaaata tttgtcgata taaatgggaa aagatgctat aatagtaatc aaatgatatg
    33601 agaaaatgta ataactgtgt aatagctatt attaatatag taaagcggga ggatatgata
    33661 tgagactgag ctttaaaatc actgttccca ttgttattct actgattgca gcaattgcat
    33721 cattatccgg agtatcattc tattttaccg gaaagttaat cgagagtaat atgtcgcaat
    33781 tgtcagggag taagttggaa gaggttcaga atataattct tcaacggaac gcagaggtat
    33841 ctatcagtag gatggagctt aaaatggaat ttttaaataa agctgagatt ctggccaatg
    33901 taattaaaca aaatcccgga tttattcaga acaattctgc tttgtttgac atgacagctt
    33961 ccttggaggt tgacgaaata catataactg atgaaaacgg aataataaga tggggtacga
    34021 ttgcaacctc ttatggtttg gatttgaatt ataatgaaag aatgaaacct tttctaccgg
    34081 cactgaccga ttccaacttt gagtttgttc aggatccaat agtaagaggt tcagataatg
    34141 ctctttttca gtatgccggc gtagccagaa aggataaacc ggggataata ctgattggag
    34201 tatcaggcct gaagcttcag agtgaattgg aaaaggcaga tataagcggc atttcaaagg
    34261 atacaagctt tggtgcaaac ggcattgtca ttattgtgga taaagaatcg gataaaataa
    34321 tcagtcataa ggatatggca atacagggcg gtaaagcagc tgaatatgat tggggcagga
    34381 aaataaggga aagtgattcc ggtgaattca agtatatttc ggatgggaca gagtatttta
    34441 cgaaatatca ggataccgga caatatatag tatgcacggt aatccctgta tcggaattta
    34501 catccgggat gagcctgttc ttgagaaata ttgtaatgat atctgcagca gctcttgtgc
    34561 tttgcatact tataatatat atactcctta aaataaatgt taccaatgaa attaacaagt
    34621 tattgaagtc attaaaaact atcggcgaag gagatcttac aaaaacaatt gatataaggt
    34681 ccagcaaaga gttttcaaag cttagcgaag gcattaacgt gatgagtaac aacctgagag
    34741 aaataataga aaagggactt gagatgacaa aaagccttag cgagtcaggt aaaaggctgg
    34801 ctgagtcagc ggatatatca agcaaaggtg cttcggaaat agcaacaaca attaatgagc
    34861 ttgcagaagg agccactgag caggcagacg gtgctacaaa aggagcgttg actgcaaagg
    34921 atgttctgag taaagctgaa gctatttccc tcaatataga ggacacagtc aaaaatactg
    34981 aacttaccaa ggataccgtt ctggcaggtg ttgaaataat caagtaccag aatgaaaaaa
    35041 tgaaggaaag tgtattaagt gcagaagacc ttggcagctc catcaatgat ttgtcaaaac
    35101 gagctgatga aatcggtgat ataacggaag tcataaccgg aatagcaaat cagaccaata
    35161 tgcttgcttt aaatgcagca attgaggcgg caagagcagg tgagatcgga aaaggatttg
    35221 ctgtggttgc tgatgaggta aggaagcttg ctgagggtgc cacgaaatca gcactcaaga
    35281 tatcggaaat aattgcagag atacagtccg gcatagggaa tgcaaagata caggcagata
    35341 aatccataag agcaattgaa gagcagcaga cagcagtaaa acagactgaa gaggccttca
    35401 tcaagataaa caatgtgact caggaagctg taaatcaggt tggcagaatt gcagaagcta
    35461 ccgagaatat tataagcggt atacataaaa tagttgatat tgtagaatct caagcagcag
    35521 tttcccagga gagcgctgcc ggaaccgaag aaataaccgc atctatgcag gaacagacag
    35581 cagccataga agaagtagca caaatagcca acgaccttgc agaggttgta ggcgagctaa
    35641 atgccttgat aagcaaattt aagatataac gaggcaaaaa gatgtccaac aggcggcgag
    35701 catatctccc gcctcttttt tatttgcatt taaattctga attaaatcat acataataca
    35761 aaaaataata caaagaagtt tttttctatc cataaacaaa attagagatg aagatatgag
    35821 tattgtttgt gaaaaggtga tagagcagtt tggtcaggat aaaaaactta ttaaccttat
    35881 tatatataat ggagaaagta taatatataa cttgaatgat gaagaattga acgcagaaga
    35941 acttcctgtg ctcaccaatt gacatttatc atatatgtcc cttccggaca gcgctgctgt
    36001 ctgtcagcaa tgaagcaagc ttcattaaat ggacaaaatc aaggtatgtt atatgataaa
    36061 cgcatggaat gaattactta ttgacgttta tcatatatgt cccttccgga cagcgatgct
    36121 gtctgtcagc aatgaagcaa gcttcattaa atggacaaaa tcaaggtatg tnnnnnnnnn
    36181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatatgata aacgcatgga atgaattact
    36301 tattgacgtt tatcatataa cgtgataaga taagattcct
//
LOCUS       DAOOAB010000023        34454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1628_length_34454_cov_16.210617, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000023 DAOOAB010000000
VERSION     DAOOAB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 34454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34454
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1628_length_34454_cov_16.210617"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1128
                     /locus_tag="PKU88_06730"
     CDS             358..1128
                     /locus_tag="PKU88_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX69015.1"
                     /translation="MIRKIVKIDEDKCNGCGLCVPNCAEGAIKIIDGKARLIADNLCD
                     GLGACLGECPRGAITITEREADEFDETAVHEHLDRKEAKPEVKQEAHSCSGHAHIGGG
                     CPGSMAREIKLRENPAEAAAVTSGDIEVKIKSQLTQWPVQLMLAPQKAPYFENADLLI
                     TADCVPFAYPNYHLELLKGKKVVVGCPKLDDIGYYMEKLTGIIRNNDIKSVTVTFMEV
                     PCCGGIVRAAETALEMSGKNIPLHKIRIKINGEAEKIS"
     regulatory      1241..1434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1565..4909
                     /locus_tag="PKU88_06735"
     CDS             1565..4909
                     /locus_tag="PKU88_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HPX69016.1"
                     /translation="MGKAYSYRRVSAIMLIMIFLTVIAPVDLYGTDYTGVLDDVVNYY
                     KSDKPGLDSWWELVALKGTGQNLSDDSWILPQWESEDLPAAAANPADYSGFILGLMAM
                     DENPQNALGGRNLTEELAGRQEEGGAFGNGAINMHIWSMVALDAAEENYDRESAVEWL
                     LSNQKADGGFALSGINGDPDMTGMALLALSPYINQADVSSAVSQAVEYLENAQCESGG
                     YSSWGIENSNSAAMVISGLVSAGQGVSSSILDALLEYRLPDNTFSYQKGGATNFLSTA
                     QSLIALGDIIENKSIFQRLSISQQPTDGEKVRIRVEGSSYSMVNKEITVTSAVYAVDA
                     LKQVLDSENITYDIQDGVWGQYINGIDGETAGKFGGYDGWLYLVNGEMASVGVGEYKI
                     EDGDELVFYYGMYPPDTLIPQVDIQPVNPKVGDNLTITVTSSYYDWNTGSTVELKVQN
                     AEISFNGKTYATDANGQAIVPSPADKGTYTLKVSKDNDGSYPSIVRIPGINVIYRANG
                     NGGGGSGSGGSGSGSGGSAETATLSVEGDSRKGTILPAVQVLLRDGDTAYSILARKMG
                     SKVKAKGSGSNIYVYEIDGLAEFDRGPSSGWMFEVNGRNPDISASAYVLKDKDVVNWY
                     YSSDSNESLGATGQTGTAQSGNSSAEISSTSNEAIKKALSENIQNSANKILTQENISD
                     WSVFALQRAGRSIPEKYIESLKVYINEHAAGSGSATDIARMVLAASAAGMNPANIDGN
                     NLIEKLFSMEKIDRQGVTGPVFALIALDSGPYSIPSGARWTRDKLVSLILEYQNEDGG
                     FCLAKDEKSDIDLTAMALQSLSGYSSESAASSVEKAIDWLSNLKPEEEQTSESISQII
                     IALTSLGKDPGSIQFGMYDGDLLTCLLKYKKEDGGFSHTLDGDSDEMATEQALMAITS
                     YSMYVNGSGKLYSLQVPNGAYSDLHAISEWALPYVAKASEQGLMEGIKTDASGQRFEP
                     RRNISRAELTALLLRILGEEPSKSFTPVYNDIKEGSWYSGYVLRAREKGIINGVTEVT
                     FDPNKPVTRQEMAVMTARAFNLGEKASGSRINDLDIASEWARQAIEAVYDNGIMMGES
                     NMFNPKGYVSREMAAAVGVRLYNMKQ"
     gene            4980..5582
                     /locus_tag="PKU88_06740"
     CDS             4980..5582
                     /locus_tag="PKU88_06740"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HPX69017.1"
                     /translation="MRRFTFLLYAVLLIILSGCGNAADADIPNSEKISKPVLSDESNC
                     IKGTKKEDIPDGKTSIKPESKSESKNNEEHVVNQEAATAKVHISITGHKDVGIILADT
                     AVNIDEGDTVIDVLKRTAELNSIQLECSGIKAMAYVKGIGNTYEFDYGPKSGWIYRVN
                     GEISNVGAGKYEVKDGDYIEWLYTVDLGREFDTESGGAQE"
     gene            5579..6457
                     /locus_tag="PKU88_06745"
     CDS             5579..6457
                     /locus_tag="PKU88_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPX69018.1"
                     /translation="MNAGFSSVHPGVCFTYYTVIMAFAMILYHPAYLASMPIALITWL
                     LLHEKGRSLSGSWHYYLLTGIAVLIINPLINHRGERVLFFLGEQAVTFEAVVYGFTMM
                     LSMLTILIAFICFRQAVDNHKFLYLFSSILPKTAFLIMMSMGFVPLLTDRMKQISIVQ
                     KARGLDMTEGTAVKRAKDGMQILKILISWSLEGALQTADSMKGRGYGIAKRSSYMKYR
                     IDGRDWLIMASIAILSAITSIGLYFGYGKLVIFPSLEIISMDITGIVFYSAFCLLLLI
                     PIIVEGREILIWHCYK"
     gene            6439..8103
                     /locus_tag="PKU88_06750"
     CDS             6439..8103
                     /locus_tag="PKU88_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPX69019.1"
                     /translation="MALLQINNFNFTYAGDTKKVLSGINLTVGQGEFIVLCGPSGSGK
                     TTLLRNIKREISPAGIKTGDILYKGMALDELKAEVSAKEIGMVFQEPDSQIVMDTVIH
                     ELAFSLENFGYAPDFINKKLSEMAAFFGLTEYLHKPVQELSGGQKQVINICSVMLTEP
                     KLLLLDEPTSQLDPVAARELISLIHQINREFSVTVIISEHRLEELFSIADRVVYMDGS
                     IIKYCNSPDGVSKEIMEAKDEYAVKSIPPIVRLFFSLSKEKGKGPGCGIPMSVREGKQ
                     FMQNIRCSVNGLTDMEIKKDWVEEIINCKDIGFRYAREEPMVLEKLTLKIMKGEFMAI
                     LGGNGSGKSTLLLAMAGLITPQQGSICVRKKNITYMKQSERNGIIGYVAQNPLLHFTL
                     DTVKEELEFALQNSLKTGSKAKMEELIELFGLKDLTDRHPYDLSGGQQQNLAVACALI
                     HEPEILLIDEPTKGLDSISKEKLAELLKSLKGKGTSVVMATHDIEFAAHYAEKCAMLF
                     DGSISDCAEPSRFFSGNYFYTTSINRAVRDYLPHAITYEDVIRACL"
     gene            8094..8654
                     /locus_tag="PKU88_06755"
     CDS             8094..8654
                     /locus_tag="PKU88_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPX69020.1"
                     /translation="MPMIRFETKKQDPRELVLISMLAAIAAISRILFAPLPNFKPMSF
                     VIIVTGIVFGPESGFMVGSVSAILSNIFFGQGPWTLLQMLAWGLMGLTAGLLRNTVWI
                     KSRYGILMFGFSWGFIFGWIMDAWYIIGFIRPLSLMTIIGGLSASFYFDLMHALANTF
                     FLGIFSKSWIKLFNRIKIKYGLLEDS"
     gene            complement(8681..9100)
                     /locus_tag="PKU88_06760"
     CDS             complement(8681..9100)
                     /locus_tag="PKU88_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69021.1"
                     /translation="MFRTNLKKRIGPLLVMLIAVMMIALGLLRISLGFAGEKDTAVIT
                     GIRRQGGERGEAIPNRYTYVVNYAFTLPDGEKIDGFTYDIRDAVYIKVSDSNIRMTPI
                     RYFKALPHINALESDSGLKAGNLILIGIGIFMIKFVK"
     gene            complement(9105..9677)
                     /locus_tag="PKU88_06765"
     CDS             complement(9105..9677)
                     /locus_tag="PKU88_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69022.1"
                     /translation="MDEYKQTKFYWEIDVPIFKNNAILKDLGIALGIPFGLLILILFI
                     ASGGDVSADGIKYPLTLIGLLFIAGFLFIMVVYGGRYSAGYLIDKNGILNYTQRKQAK
                     KNLIVNSLLIIIGLFSKRPSAAGAGLLAQTRQSVFIAWKSIRKVKVYPKSRSIVVIGG
                     FAEKIALFCNEDNFHLVSDIILSKFNANGE"
     gene            complement(9765..10256)
                     /locus_tag="PKU88_06770"
     CDS             complement(9765..10256)
                     /locus_tag="PKU88_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69023.1"
                     /translation="MNTIKRKLMIYMMLSSFGILISFTLFAGYFHIVVVFSILTTILL
                     LVFSLNEYRRYAFARLIIENKIIHIEAAETESESKMNKSIEIFVSCFGILLDSKIIKF
                     NTEGISLKKVEVGHDYILLAYGKGEKYKTIRLLHSNINEPELRDIIEKFRRETGIIPE
                     LAG"
     gene            complement(10330..12078)
                     /locus_tag="PKU88_06775"
     CDS             complement(10330..12078)
                     /locus_tag="PKU88_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HPX69024.1"
                     /translation="MKKFLSIVTVLALLFSMAAGFTTVVSAAEEGYAWVLVDTHEYPV
                     PEADSFYGSLKGNTIELQYAWAYGLGIYASKDYDNPTDMHAIYTWSNPPSTIMPNQTV
                     TLKLEQNVISNRNGNYSIGFNPYFHMDMEDIDIGYATASKVIAKITYADGTTAKYPGI
                     GYNQDAQAQQSFTADMTLNFIGEGRPGDKHSLYVGVYAGSPGSVGVRYTYEWKQTSSA
                     NAAPISSGTTDLGTYSWAGEWDSNWGKMLITQNGASVTGTYTHDSGKINGTISGNVFT
                     GTWSESPSYSPPSDAGDMELTMSADGKSFTGRWRYGSEGSWSNWDGSMRITEVIPAPK
                     PAATSPAPVITTTQANSAGSNAETFESGSRIMWQPADGLGYRLFRSTSKSDLGISVTD
                     FYITGTSYADVNIEANTTYYYTVKPVLAEAKPFEGIDEKLGAAIATYTVTTGSQVYKP
                     GSFKHFIMLKLDNPNMSVDGVIQEVDPGRSTSPMTIAGRTMVPIRAVVEAMGGTIGWE
                     QDTRKITLTARGNVVEMWLDKTDIKINGVSQKMDVAPVSKNGRTFVPVRFAAENLNCK
                     VDWINSTKEAVIVYEE"
     gene            complement(12247..13647)
                     /locus_tag="PKU88_06780"
     CDS             complement(12247..13647)
                     /locus_tag="PKU88_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX69025.1"
                     /translation="MLRDERSKTIIAFSSFSSWLLSFLFGGQVLLTIASINNVNVNEL
                     TFGAIAAHFIGLFACGFFIKSTKAAKGLMLFSIPVCILGTVVFFFIPSIMWHISLLIL
                     SFLSGACVAAWGFYFKMLAFSYERIKIIADVLIYSNILMVIINTAAVLISSYLGLALS
                     ILMLSISLFFHTKLAVDQNTAEERHTRQPIKAVSPIKPLAFLCLFIVIITINSGLMYQ
                     VINPAFAHHEWLVSWYWALPYIAALYIVRNLSGKANRSYILYVAIAMIGLSSVGFMIL
                     DRSAASYLIIDTLMLGACGVYDLFWWSILGEMLDYHHNPAKILGIGLSANVFGVLIGG
                     MFGSTIADSDTGSIDSSVLALVVVFIVLILLPLLHRHLSGLLKNHIFLTALSEIEPSK
                     QKKAIENFTVIGRLTERESEIAALLLKGRTYKMIADELCLSENTVKTHIKNIYSKFNI
                     QSKTELIKLLTGKETH"
     gene            complement(13793..13975)
                     /locus_tag="PKU88_06785"
     CDS             complement(13793..13975)
                     /locus_tag="PKU88_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69026.1"
                     /translation="MNIIDETYNGEKYPIIERKYNSYWFPENYNPDNEEAQAEELVNS
                     YMNHEEIINNKNKNSK"
     gene            complement(13972..14157)
                     /locus_tag="PKU88_06790"
     CDS             complement(13972..14157)
                     /locus_tag="PKU88_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69027.1"
                     /translation="MSTKINKKLNVLTPAAEEPITDYEIVMAKINHDSHKKYNGIENI
                     ALENEDIKEKDKDEVKP"
     gene            14309..16234
                     /locus_tag="PKU88_06795"
     CDS             14309..16234
                     /locus_tag="PKU88_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPX69028.1"
                     /translation="MNYLENLNKEQREAVLHTEGPLLILAGAGSGKTRVLTYRIAYLI
                     DEKGIYPSNILAITFTNKAAREMKERVHRLIGNTDNMWISTFHSACVRMLRKNVESLK
                     GYKKNFVIYDTKDQETLIKECLKELNLNEKNFPFRVVSAEISAAKDRLIPPREFYDKN
                     MHDIRRRKLADIYMLYQKKLEKNNALDFDDIISKTVELLKLNPDILEYYQNKFRYILV
                     DEYQDTNFSQYTFIRLLAAQHRNICVVGDDDQGIYSFRGADIGNILNFEKDFPGAKVV
                     KLEQNYRSTETILEAANAVISNNYGRKSKKLWTDKGTGRSIIYHYAVDEREEANFIMD
                     EVERMIAQENRKLGEFAILYRTNAQSRVLEETCMSHGLPYRIVGGFKFYDRKEVKDII
                     AYLRVIQNPDEDLSLKRIINVPKRGIGNSTLEAIAQYARTTGDSIFGALLEVDRIEGV
                     STKAKKGIKEFVKIMSDLMGIAETEMVSIIVKEVLERTGYLDQLEKDGDEESLMRAEN
                     IKELLSATFEFEELNESALLPEFLEQMALMSDIDAVEEGKDALIMMTLHSAKGLEFPF
                     VFISGMEEGVFPSQRSYFEDNQMEEERRLMYVGITRAKERLYLTAAFERTLYGNTTFN
                     TISKFVREIPKELLVKV"
     gene            16486..18486
                     /gene="ligA"
                     /locus_tag="PKU88_06800"
     CDS             16486..18486
                     /gene="ligA"
                     /locus_tag="PKU88_06800"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPX69029.1"
                     /translation="MGIDKLEKRADELIKLINRHNYNYYVLDKPEISDYEYDLLMKEL
                     IELENTFPALKRADSPTQRVGGEVLKGFAEVVHKTPKLSLSNVFDENDIRDFDYRVRK
                     AVGGDVEYVVELKIDGLTVVLNYEEGRFVLGATRGDGIKGEDITSNLKTVKSIPLLLE
                     EEVDLEVRGEVFMSKKAFEELNARREEQEEPLFANPRNASAGSLRQLDTKVTAGRPLD
                     IFIFNLESIEGKNFDTHVETLEYLKELGFKVSPFISVHKSSEEIIEKCKYWADKRGEL
                     PFVIDGLVIKVNNLRQREAMGYTTKNPRWATAYKFPPETKKTKVKDIIVQVGRTGAIT
                     PTAELEPVRLAGSVISRATLHNEDYIKEKDIRVGDTVLIKKAGDVIPEVVEVVREDRD
                     GSQQPFEMPKKCPVCQSDTIREQGEAVTKCTNISCPAQLKRALFHFVSRDAMNIEGFG
                     PQIMSLLMDKDFIRDAADIYLLKNHRDELVEIERMGEKSVQNLLDAIEASKVNPLNRL
                     IYALGIRMIGQRASQVLANEFEDIEAMFDADYERLTGISEIGDKMAESIITFFKQEQN
                     IRLIRKLQECGVNTKGSKKEIKENEHFKGKTFVLTGGLANFTREQAKEIIESFGGKVS
                     GSVSNKTDYVLAGENAGSKLDKANELNVAVIDEDTFRSWAEI"
     gene            18922..20577
                     /locus_tag="PKU88_06805"
     CDS             18922..20577
                     /locus_tag="PKU88_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX69030.1"
                     /translation="MKKSLVLVLAMLMILSFALSACAPKAPAETPGAETPAPAAEPKI
                     FRTNNMSEPGSLDPALAQGTHESWILDHAFEGLMARDPSGISVPGMAESYTLADDEVT
                     YTFKLRDGITWSNGDPVTAYDFEFAWKRALDPDLAADYAFQLYYLKGGEAYNTGAGSV
                     NDVGVKAIDDKTLEVVLATPTAYFLELTSFYTYYPVNKKVVEANADWAKSADTHVSNG
                     PFKLTGWEHNATITLAKSENYYNKEAIKLDGIELAIIADENTAWQKYLGGEFDFLTPL
                     PQAVTAQLKAEGNPELVIGGEVGTYYYNLNNKLKPFDNAKIRKALSLTLDRQTICDKI
                     AQGGQLPAEGVVPFGMLDENNKEYRDGAGKLVEYNVEEGKKLFAEGLAETGFKVEDFS
                     KFTLLYNTSEAHKIIAQAAQEMWRTNLGIELTLENVDFQVKLDREKAGDFQISRAGWI
                     GDYIDPMTFMDLWLSEGAFNDAKYNNPEYDRLIKESKATTDQKVRMENMRKAEQMLME
                     AMPVVPVYFYTQPYAQKPYVTGVFKPLNRYPIFTFADMDMSKR"
     gene            20677..21606
                     /locus_tag="PKU88_06810"
     CDS             20677..21606
                     /locus_tag="PKU88_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69031.1"
                     /translation="MKKYLIKRVLMSVVTIWAIITITFVLMHSVPGNPFRQENKMPKV
                     VYENLLKKYGLDKPLSEQYFIYLGKIVKGDFGDSMKSRVETVNDMIRRGFPVSAVIGA
                     EALLIAIIFGPALGAIAALNQNRWPDYLSMIIAIIGISVPSFIMGTVFIQFLARNIEW
                     LPIGGWGSFRHSILPSLALALMPLAQLARFMRSSMLEVMGQDYINTAKSKGISKATVV
                     LRHAVRNAILPVVSVLGTVATNLLVGSFVIEKIFGIPGLGMFFIISINNRDYPLIMGT
                     TIFYAIILIVMLLIVDIAYMFIDPRIKLAKEGR"
     gene            21603..22550
                     /locus_tag="PKU88_06815"
     CDS             21603..22550
                     /locus_tag="PKU88_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69032.1"
                     /translation="MMITEFEYSPELFELASKEDRNAEKIRRPSIKYWPDVWRRLKLN
                     KLAMVGLSLIVFFGIMAFVGTQINGYTYFEQNYSLINVHPDSVHWFGTDELGRDLFTR
                     TWFGVRYSLFIGVAAAFIDFSIGVLYGGIAGITTRRIDAVMMRIAEVIYSIPYLLVVI
                     LISVVFSAAGQGSSMFVLILALSLTSWVPMAILVRGQVLQLKESEYSLASESLGADKK
                     WILRKHIIPNTLGPILVNITLSIPRAIFAEATLGFIGLGLQAPKSSLGTLANSGLAGL
                     PVGNSYQLFIPAIFISLIMFAFNVLGDGLRDALDPRLRK"
     gene            22565..23575
                     /locus_tag="PKU88_06820"
     CDS             22565..23575
                     /locus_tag="PKU88_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69033.1"
                     /translation="MENVLEVKNLKISFQTFFGEVEAVRGITFSVGKKETLAIVGESG
                     CGKSVTANGIMQLLPKPPAFFKGGEILFNGEDLLKKTEKQMQAIRGNKISMVFQDPMT
                     SLNPTMKIGYQIVEGLVKHNKMSREEAKKKSIEMLELVAVPQPEKRINQYPHEFSGGM
                     RQRVMIALAMVCNPQLLIADEPTTALDVTVQAQILELMKKLQDQMGMSIILITHDLGI
                     VADMSDRVVVMYAGQILEVGLTDEIFAAPKHPYTRKLLASVPRLDMSREEPLYSIDGT
                     PPDLYVPPKGCSFYDRCSEAMKICKNNMPEFDEHSATHQSRCWLNHPMAQKTNSTDGR
                     EV"
     gene            23572..24528
                     /locus_tag="PKU88_06825"
     CDS             23572..24528
                     /locus_tag="PKU88_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX69034.1"
                     /translation="MMTEPLISARNIKKYFDVKGGKLKAVDGISFDIFPRETFGLVGE
                     SGCGKSTAGRTIVRLYDPTEGELIFNGQNIYKLSRKDMQEIRRDFQMIFQDPYASLNP
                     RMTVEDIVAEPLDIHQTYKNRAERTERVIELLRLVGLNDEHAMRFPHEFSGGQRQRIG
                     IARALALNPKFIVCDEPISALDVSIQAQVVNLLKELQDGLGLTYMFIAHDLSMVRYIS
                     DRVAVMYLGHMMELAESDELYSNPVHPYTKALLSAIPIPNPEIQRNKKRIMLEGDVPS
                     PINPREGCRFVDRCQYAIGRCREETPVFEEKAANHFAACHVV"
     gene            24658..25326
                     /locus_tag="PKU88_06830"
     CDS             24658..25326
                     /locus_tag="PKU88_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69035.1"
                     /translation="MTLEDYLEEGCVYHVVSIIDLKKTLHEGIRYDDKNTYACKYTDF
                     HSFFDLYKPKGIPDWVERRKAIFASIGFKEGHKWHSHSAILRIRILKDRCWICNENLA
                     NFIYEPFILQHMDGFEGTKEFMKANGKAFAGKYWQNSLSFNDNLIKRYDKREGYDAEV
                     LVMHPIPPRNIECLYIVSDHRIMSYQAWKEYFTPGSPHAEVQYNRYLQPDNMSSAWKY
                     PGSQ"
     gene            complement(25260..26627)
                     /locus_tag="PKU88_06835"
     CDS             complement(25260..26627)
                     /locus_tag="PKU88_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69036.1"
                     /translation="MIFEFSYPDIIKQCNTMTYLNVISFCLLSGCGSIEMPDEQELSH
                     IYKLLKGAGSIRMPKNITFMSSLYKRCIPIYAAIPEASYDFKSFRWLEKKMKKSVEPN
                     VLAHSICSMTALIPIIQNGEIQMENKEFIAYCLGVNSIKQARFLIDYLKLGDFYYSGE
                     DTGDNVYGEYRITINNENPDIKTQFFVAEALSSILGLIDRDDSYSRDLVPRLKKALEV
                     LPVLCENITENINDISSRDLSVVGLCLLSILGHTAVYKETVYSTANVIGMELCERLAH
                     TGDIARNISDDSFSSFITLCNCMNFLIKLHEVNDLIVYEASFLKLYDRIDSYWDRSSG
                     LFLVTRKNKQKYSFKETAAVFAALCMLRSCLTDAGLFMHVDRQLSAFYSATFISSKLF
                     NNQFYPILQDTKLELHNLGSTEKRSAPVFSEYFEVKLNKRKYHCEPGYFQAEDILSGC
                     KYLLY"
     gene            26816..27796
                     /locus_tag="PKU88_06840"
     CDS             26816..27796
                     /locus_tag="PKU88_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HPX69037.1"
                     /translation="MKEGKIPPELLKSLVFNNIKVRNEEVILRPEIGEDCAAIDFGSH
                     ACVLSTDPITGAVKNAGALAIHISCNDVAASGVRPVGIMITILAPPEAAEGDIRRVME
                     EAGEAASKLGVEIIGGHTEVTSAVNRIVISTTALGKVRKDRIVRSSGAKAGDDVIMTK
                     WAGLEGTSIIASDKGKELEAVLTPEEIESARSFIDNISVVEEGVTAGEFGANSMHDVT
                     EGGILGAAWEIAESSGKGIDIYIDKIPVWEVTKKICSFYGIDPYRLISSGSMLITASK
                     GAELAKLLEKKRIPAAIIGRITEAGRNIIRNGNISLLNPPDTDEIYKALK"
     gene            28163..30622
                     /locus_tag="PKU88_06845"
     CDS             28163..30622
                     /locus_tag="PKU88_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HPX69038.1"
                     /translation="MKKVFVVITVLCLLLTSFAGTVSAQASEAKAPPLSMQILGKKVS
                     TASPPIVKNNRIFLPVRAVGEALGYKATWNAQTSTMELKDSKQTLKIKVGSTDAYVNG
                     NKVKLDAAPIMYKERSYMPLTFVQKHFNYTTTYDKTKNIISIDKKTEADKTAAQTQGL
                     YVLGKKIGTDAPVIKNNAVYIPLRHAGEGMGYKVSWYAGPETMEMRLGDNLVKVTVGY
                     GFGVVNGKNVKISPVPIMYNSRLYVPITFIQNNFDYDVSYDKAKNAASIDKKKPPAAE
                     AEEPIEYKKAKIEEIIYDDNGGFPQLNIVADNPVKYKSFTMVNPDRMVVDIENAVLNT
                     EFETKEIGKGGVIRARVAQFSKSPDVVRVVIDLENQQLCKLVQSNDKKTISLIYANII
                     IPVSFVREEGRDVLMIRGSQEMDTNVFTLENPDRLVVDIRKSVLSEKGQTVETNTPFV
                     KTIRTGQVDVGVARVVLDVEPGIYYDVTNNGSLTKVYISDVPFSFFDYNKYYNSAYVD
                     LSPGAEVEYQPIIDKANRTLKIMIPKDIDVEEKVHDINDNVLENVTITKETYGDQIFT
                     AAAFKMKPSVEYELLSPAVTKLVSLKFKYRALKSGQLTVLIDPGHGGKDPGATAIDKK
                     TYEKGLNLDVALRLNRILKGIGFKTIMTRTDDSYVDLASRSGLANNSYVDFFMSIHFN
                     AFNSTAKGIETLYFPNEVTDEYQLDNREIANIFHSELIDALNRTSRGITPRPGLHVLN
                     KTKMPAILAELGFLTNREDFAEIKKDEYREMAARALAVSIVKYFQDIENINLGIDTNA
                     IYAADAPKDPYSLEQDLASNN"
     gene            30818..31870
                     /locus_tag="PKU88_06850"
     CDS             30818..31870
                     /locus_tag="PKU88_06850"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPX69039.1"
                     /translation="MNRNLALLLALLICCTLVLTSCSNPLISLWDFFNKKTDTVEESP
                     QQVQEETQSMSEVSVSQPEDSRATVLYYKDNEDFLIPVMRYMPKGDLGVAKTAISAII
                     YSPETVEDLKAAGLTPTLPMGTKINGAVIRENGLAIIDFSKEFLNFSSKKAEELGIKA
                     VTYTLTEFPNIKSVEIRVDGKKIEEMPKGTEVGSELKRTEINLQPVEKAGEKLSKVVV
                     YYHKKGSGAYSYFVPLTKVVSGFENSVEAALSALLEGPEAGSLKNPFPEGTKLLDVQV
                     NSGIAYVNFSEHILANQGDKAAENAVKKAVALTLKEFPAISKAKIFVNGKIVENTAGV
                     GADEYISVPVFVNFYE"
     gene            32103..32870
                     /gene="rph"
                     /locus_tag="PKU88_06855"
     CDS             32103..32870
                     /gene="rph"
                     /locus_tag="PKU88_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPX69040.1"
                     /translation="MQRIGGRNNEQIRPVKIIRNYLKHAEGSVLIEMGDTKVICTASV
                     DDKVPPFIKGTGKGWITSEYGMLPRSTEVRKPRESARGRIDGRTMEIQRLIGRALRSI
                     VNLEKLGEITIYIDCDVIQADGGTRTASITGSYVALVDALYKLVQQERLKEMPLNGYI
                     AAVSVGIKDGEAILDLNYSEDSTCQVDMNLVMTDRGEFIEIQGTGEESPFSKVQLQEM
                     MELAEKGIRELIEIQKRSLGEIADKVGTYDENDSSDK"
     gene            32842..33438
                     /gene="rdgB"
                     /locus_tag="PKU88_06860"
     CDS             32842..33438
                     /gene="rdgB"
                     /locus_tag="PKU88_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPX69041.1"
                     /translation="MMKMIAATNNSHKLEEIRAILKDKGIEVISLADAGLDIEIEENG
                     DSFMGNALIKAREVFRLTGEAAIADDSGLEVDALKGQPGVKSARYSGESGADKDKKNN
                     EKLKEALKDIPDNERGARFCSAIAAVFPRGKEITAEGYVYGKIGYEEKGEHGFGYDPL
                     FIVDGYGRTMAELDSEEKNKISHRANALKEFVKKLDML"
     gene            33539..33612
                     /locus_tag="PKU88_06865"
     tRNA            33539..33612
                     /locus_tag="PKU88_06865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33571..33573,aa:Gly,seq:tcc)
     gene            33618..33694
                     /locus_tag="PKU88_06870"
     tRNA            33618..33694
                     /locus_tag="PKU88_06870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33652..33654,aa:Arg,seq:tct)
     gene            33704..33780
                     /locus_tag="PKU88_06875"
     tRNA            33704..33780
                     /locus_tag="PKU88_06875"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33738..33740,aa:His,seq:gtg)
     gene            33791..33866
                     /locus_tag="PKU88_06880"
     tRNA            33791..33866
                     /locus_tag="PKU88_06880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33823..33825,aa:Gln,seq:ttg)
     gene            33877..33952
                     /locus_tag="PKU88_06885"
     tRNA            33877..33952
                     /locus_tag="PKU88_06885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33910..33912,aa:Lys,seq:ttt)
     gene            33959..34042
                     /locus_tag="PKU88_06890"
     tRNA            33959..34042
                     /locus_tag="PKU88_06890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33993..33995,aa:Leu,seq:tag)
     gene            34049..34122
                     /locus_tag="PKU88_06895"
     tRNA            34049..34122
                     /locus_tag="PKU88_06895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34081..34083,aa:Gly,seq:tcc)
     gene            34128..34204
                     /locus_tag="PKU88_06900"
     tRNA            34128..34204
                     /locus_tag="PKU88_06900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34162..34164,aa:Arg,seq:tct)
BASE COUNT        10590 a         6474 c         8268 g         9122 t
ORIGIN      
        1 aaaaattgat ggctctaatt ccactcaact aagaaaaacc taacgctgtc aaaggtcgtc
       61 cgtgaccgtg acagcttgct cggcctccat gcctcgcata cggttgtttc taagtcgagc
      121 gagatttgag ccattaacaa tttttgcata agcatgctct gactatacat tgctgttcct
      181 attctgagat ttgtctgcaa actgaaagtc ctgaaaaggg ctttattttt tactataaag
      241 atttttttcc gccggtattt tgtgattaat aatacacgct taagtgacat atatcacagc
      301 taagttattt taacggggat ataataaaac caaagaaaaa atttggaggt aaataaaatg
      361 ataagaaaaa ttgttaagat tgatgaagat aaatgcaacg gctgcgggct ttgtgtgccc
      421 aactgcgctg aaggtgccat aaagataata gacggaaaag caaggctcat agcagataat
      481 ctttgcgacg gcctgggtgc atgtcttgga gaatgtcccc gcggtgcaat cacaattact
      541 gaaagagaag cggatgaatt tgacgaaaca gctgtacatg agcatcttga caggaaggaa
      601 gcaaaacctg aagtgaagca ggaagcccat tcctgcagcg ggcatgcaca tattggcggc
      661 ggatgcccgg gaagcatggc aagggaaata aaattacgcg agaatcccgc agaagcagca
      721 gcagtgactt ccggagatat cgaagtaaag attaagtcac aactgactca gtggccggta
      781 caattgatgc tggctcccca aaaagcaccg tattttgaaa atgcggacct tttgattact
      841 gcagactgtg tgccctttgc ttatcccaac tatcatctgg agctgttgaa gggtaagaag
      901 gttgtagtgg gatgccccaa gctggacgat ataggctatt atatggagaa gcttacaggg
      961 ataataagaa ataatgatat aaaaagcgta acagtgacat ttatggaggt cccctgctgc
     1021 ggcggaattg taagggcggc tgaaactgcc ctggagatgt caggcaagaa tattcccctt
     1081 cacaagatca gaataaaaat caacggagaa gctgagaaga tcagctgaat ccgttaaaat
     1141 acaggggcgg ccatcatcgg ccgccctgtt atgcaatata tttttattta tatatgataa
     1201 tactattgat aggattgaat gaagcatata taataaaact ataacttaat actgatcagg
     1261 tgccggtttg tttatgaata atctgccggc taaaagggaa gtcgggtgaa aatcccgcgc
     1321 ggtcccgccg ctgtaaaaga ggagttctcc ttaagatgtc actgagtaga ttgggaaggc
     1381 aaggagaatg atgatgcttg agccagaaga cctacctggt ttataacact aaatactcta
     1441 cgagagatag gggggtgttc ttatatgcag gatgattata gtcattttgt cacataacct
     1501 cttaactatt tagttaagag gtttatcttt ttattaagaa agaaagcatg gaggttgtat
     1561 ttgtatgggc aaggcgtatt catacaggcg tgtatcagct attatgctga taatgatatt
     1621 tctaacggta attgctcctg ttgatttgta tggaactgat tatacaggag tgcttgacga
     1681 tgttgttaat tattataagt cagataagcc cggccttgat tcatggtggg agcttgtggc
     1741 gttaaaaggt acgggacaga acctgagtga tgacagctgg atacttcctc agtgggaaag
     1801 cgaggatttg cctgccgcag ctgcaaatcc ggcagattat tcaggattta tactggggct
     1861 tatggcaatg gatgaaaatc cgcaaaatgc ccttggaggc aggaacctga cggaggaatt
     1921 ggcaggcagg caggaggagg gcggcgcttt tggcaatggt gcaattaata tgcatatatg
     1981 gtcaatggtt gcgttagatg cagcagagga gaattatgac agggaaagcg ccgttgaatg
     2041 gttactgagc aatcagaagg ccgatggggg ttttgctctg tccggcatca acggcgatcc
     2101 tgatatgaca ggaatggcgc tgctggcatt atctccatat ataaatcagg ctgatgtaag
     2161 cagtgctgta tctcaggcag ttgaatatct ggaaaatgcc cagtgcgaat caggaggcta
     2221 cagctcatgg ggcatcgaaa attccaacag tgcggcgatg gttatttcgg ggcttgtttc
     2281 tgccggccag ggtgtgagca gctccattct tgatgcgctg ctggaatata ggctgcctga
     2341 caatacattc agttatcaga aaggcggcgc tacaaatttc ctttctaccg cacagtccct
     2401 tatagcactg ggagacataa ttgagaataa gagtatattc caaagattga gcatttctca
     2461 gcagcccacg gatggagaga aagtcaggat tcgggtagaa ggcagcagct atagcatggt
     2521 aaataaggaa ataacagtaa cctctgcagt gtatgcggta gatgccttga agcaggtatt
     2581 ggattccgaa aatatcacct atgatataca agacggcgtg tggggacagt atattaatgg
     2641 cattgacgga gaaaccgcag gaaagttcgg tggttatgac ggttggttat atctggttaa
     2701 cggagaaatg gcatcggtgg gtgtaggtga atataaaatc gaagacggag atgaactggt
     2761 attttattat ggcatgtatc caccggatac tttgattccc caagtggata tacaacctgt
     2821 aaatcctaag gtaggagaca atttaacgat tactgtcact tcaagctatt atgactggaa
     2881 taccggcagt acggttgagc ttaaagttca aaatgctgaa ataagcttca acggcaagac
     2941 ctatgccacg gatgccaatg ggcaggcaat tgtgccaagt ccggctgata agggtacata
     3001 caccttgaaa gtaagtaagg ataacgatgg cagttatccc agcatcgtcc gcatcccagg
     3061 tattaatgtt atatataggg cgaatggtaa tggcggcgga ggcagcggca gcggaggcag
     3121 cggttccggc agcggcggct ctgcagaaac tgcaacatta tcagtggaag gtgacagccg
     3181 taaaggtacg atattgcctg ctgtccaagt attattgcgg gatggggata ctgcttacag
     3241 catacttgca agaaaaatgg gaagtaaggt aaaggctaaa ggctccggct caaacatata
     3301 tgtctatgag attgacggct tggctgagtt tgacaggggt ccgtcaagcg gctggatgtt
     3361 tgaggtcaac gggagaaatc ctgatattag cgcttctgcc tatgtattga aggataagga
     3421 tgttgtcaat tggtattatt catccgattc caatgagagc ttgggtgcaa ccggtcagac
     3481 aggaactgca cagtcaggga atagcagcgc ggaaatcagc agcacgtcaa atgaggcaat
     3541 aaagaaagca ttgtcagaga atattcagaa ttcagctaat aaaatactca cacaggaaaa
     3601 catttcggat tggtctgttt tcgcactgca aagggcaggc cggagcatac ccgaaaaata
     3661 tattgaatcc ctaaaggtat acattaatga acatgcagcg ggttccggaa gtgcaaccga
     3721 tattgcaagg atggttctgg ctgcaagtgc agcaggaatg aatcctgcga acatagacgg
     3781 aaataatctg attgaaaagc tgttcagcat ggagaaaatc gacaggcagg gagtcacagg
     3841 gccggttttt gcgctgatag cattggacag cggaccttac agtattccgt caggcgcaag
     3901 gtggacgaga gataagcttg tttcactgat acttgaatac cagaatgaag acggaggctt
     3961 ctgccttgca aaagatgaga aaagcgacat agatttgaca gctatggcac tgcaatcact
     4021 gtccggttac agcagcgaaa gcgctgcttc atccgtagag aaagctattg attggttatc
     4081 taacctgaaa ccggaagagg agcaaaccag tgagagcatt tcacagatta tcatagctct
     4141 tacatctctt ggtaaggatc cgggaagcat acagttcggc atgtatgatg gggatttgct
     4201 gacctgcctg ctgaaatata aaaaggagga cggcggattt tcacatacat tggatggaga
     4261 cagtgatgaa atggccacgg aacaggcttt aatggccatc acttcataca gcatgtatgt
     4321 taacggttca ggcaagcttt attccctgca ggttcctaat ggggcatatt cagaccttca
     4381 tgcgatttcc gaatgggcac tgccgtatgt tgccaaggcc tctgagcagg gactgatgga
     4441 agggataaaa acagatgctt cagggcaaag gtttgagccc aggaggaaca tttcaagggc
     4501 agagcttact gcgctgcttt taaggatttt gggagaagaa ccgtcaaaga gctttacgcc
     4561 ggtatacaat gacataaaag agggttcatg gtattcagga tacgttctga gggccagaga
     4621 aaagggtatc attaacggtg ttacagaagt caccttcgac cccaacaaac cggttacaag
     4681 gcaggagatg gctgtcatga cggccagagc ttttaatctt ggtgaaaaag catcaggcag
     4741 caggataaat gacttggaca ttgcttcgga atgggcaagg caggctattg aggctgttta
     4801 cgataacggt attatgatgg gggaaagtaa tatgttcaac ccgaagggtt atgtcagccg
     4861 tgaaatggca gcagctgtcg gagtaagact ctataatatg aagcaataat aatattaagc
     4921 ctcctgcctg taaaaataca ggcaggagca aaatccatta tttaatgcgg gagagtaata
     4981 tgagaagatt cacattttta ttatatgcag tacttctgat aattttatca gggtgcggca
     5041 atgcagcaga tgcggacatt ccgaattcgg agaagatttc aaagccggta ttgtctgatg
     5101 aaagcaattg cataaagggt acaaaaaaag aagatatccc ggatggtaaa acaagtatta
     5161 aaccggagag taaatcggaa agtaaaaata atgaagagca tgtagtaaat caagaagcag
     5221 caactgccaa ggtacatatt tccattacgg gacataagga tgtggggata attcttgcag
     5281 atacggcagt aaatattgat gagggggata cggtaattga tgttttgaaa agaactgcag
     5341 aactaaacag tatacagctg gaatgcagcg gaatcaaagc catggcatat gtaaaaggca
     5401 ttgggaacac ctatgaattc gattacggtc ccaagagcgg ctggatttac agagtaaacg
     5461 gggaaatatc caatgtaggt gcgggcaaat atgaagtgaa agacggagat tatatcgaat
     5521 ggctgtatac ggtggattta ggaagggagt ttgatacgga atcaggagga gctcaggaat
     5581 gaatgcagga ttttcgtcgg tacatcccgg agtatgcttt acatattata ccgtaataat
     5641 ggcttttgca atgattttgt accatcctgc ttacctggcc tcaatgccca tagctttgat
     5701 aacctggcta ttgctgcatg aaaagggcag aagcctgtcc ggttcatggc attattacct
     5761 gctgacaggt attgcggtac tgataataaa tcctctcata aaccacagag gggagagggt
     5821 tctgtttttt ctcggtgaac aggctgtgac ctttgaagct gttgtatacg gattcactat
     5881 gatgctgtcg atgctgacaa tacttattgc atttatttgt ttccgtcagg cagtggataa
     5941 tcataaattc ctgtatcttt tctcatccat ccttcccaaa acagcattcc tgataatgat
     6001 gtcaatgggc tttgtccctt tgcttactga caggatgaag cagatatcca ttgttcagaa
     6061 agccaggggg ctggatatga cggaaggaac tgccgtaaag agggcaaagg acggtatgca
     6121 aatattgaag attcttattt cctggtctct tgaaggagca ctgcaaactg ccgattcgat
     6181 gaaagggaga ggctatggaa ttgcaaaaag gagcagctat atgaaataca ggatagatgg
     6241 cagggattgg ctgattatgg catcaattgc aatactatcg gctatcactt ccattggttt
     6301 atattttgga tatggaaagc ttgtgatttt tccgtccttg gaaatcataa gcatggatat
     6361 taccggcatt gttttttatt ctgccttctg tttgctgttg cttataccaa taattgttga
     6421 gggaagggaa atcttaatat ggcactgtta caaataaaca actttaattt tacttatgcg
     6481 ggagatacaa aaaaggttct ttcaggaata aatcttaccg tcgggcaagg agagtttatt
     6541 gtattatgcg gaccatcagg ttcagggaaa acgacacttc tccgtaatat aaaaagagag
     6601 ataagtcctg cgggcattaa aaccggagat atactgtaca aaggtatggc cttagatgaa
     6661 ttaaaggctg aggtaagtgc aaaagagata ggaatggtgt tccaggaacc ggacagccag
     6721 attgtcatgg atacggtgat tcatgagctg gccttttccc ttgagaattt tgggtatgca
     6781 cctgatttta taaataagaa gctatccgaa atggccgctt ttttcggatt gactgaatat
     6841 ctgcataagc cggtgcaaga actgtcagga ggccagaagc aggtaataaa catctgttct
     6901 gtcatgctaa cagagccgaa gctgctttta ttggatgagc ctacatctca gcttgatccg
     6961 gtagcagccc gtgaacttat aagcttgatt catcagataa accgggaatt ttctgttact
     7021 gtaataatta gcgaacacag gctggaagag cttttttcaa tagcagaccg ggttgtttat
     7081 atggatggga gtattattaa gtattgcaat tctccggatg gggtatcaaa ggagattatg
     7141 gaagccaaag acgaatatgc agttaaatct atccctccaa tagtacgttt gtttttcagc
     7201 ctttcaaagg agaaaggcaa aggcccaggg tgcggcattc ccatgagcgt aagagaagga
     7261 aagcagttta tgcaaaatat cagatgcagt gttaacgggt tgactgatat ggaaatcaag
     7321 aaggattggg ttgaagagat cattaactgt aaggatatcg gctttagata tgccagggaa
     7381 gagcccatgg ttctagagaa actgacactt aaaataatga aaggggaatt catggctata
     7441 ctgggcggca acgggtcagg caaaagcacg ctgctgctgg cgatggccgg gctgataacc
     7501 ccgcagcagg gcagcatctg tgtcagaaag aagaatatca cctatatgaa acagtctgaa
     7561 agaaacggta taataggcta tgttgcgcaa aatcctttgc ttcatttcac attggataca
     7621 gtaaaggagg agcttgaatt tgcattgcag aactcattaa aaaccggaag caaagcaaaa
     7681 atggaagagc ttattgaact attcggcttg aaggatttaa cggacaggca tccctatgat
     7741 ttgagcggag gccagcagca aaatctggct gtcgcatgtg ctttgattca tgaaccggag
     7801 atactgttga ttgatgaacc taccaagggg cttgacagca tatcaaagga aaaacttgca
     7861 gaattgttaa aaagccttaa gggtaaaggt acttccgtag ttatggcaac ccacgatatt
     7921 gagtttgcag cacactatgc tgaaaaatgc gcaatgctgt ttgacggttc tatatcagat
     7981 tgcgctgaac cttcaaggtt tttcagcggc aattactttt atactacttc gattaaccgg
     8041 gcggtaaggg attatctgcc tcacgcaata acatatgagg atgtgataag ggcatgccta
     8101 tgataagatt cgagacaaaa aagcaggacc cgcgggaatt ggttctgatt tcgatgcttg
     8161 cagcaatagc ggcaatcagc agaatattgt tcgcacctct gccgaatttt aaaccaatgt
     8221 cttttgttat tattgttacc ggcattgtat tcggcccgga atccggcttc atggtgggat
     8281 ctgtatcggc aatcctgtca aatatttttt tcggccaggg tccgtggact ttactgcaga
     8341 tgcttgcctg gggattgatg gggcttacgg ccggactttt aagaaatacg gtctggatta
     8401 aaagcaggta tggaatatta atgtttggat tctcctgggg attcatattc ggttggataa
     8461 tggatgcatg gtacatcata ggattcataa ggccgttgag cctgatgaca attattggag
     8521 gcctttctgc tagcttttat tttgatttga tgcatgcgct tgcaaatact tttttcctgg
     8581 gaattttttc aaagagctgg ataaagctgt ttaatcgcat aaagataaaa tacggtttac
     8641 tggaagactc ttagatatat aatgctatat cggaggaacc tcattttacg aattttatca
     8701 tgaaaatacc tattccaatt agtatcagat tgcccgcctt tagcccggaa tcgctctcaa
     8761 gtgcgtttat atggggtaaa gccttgaagt atctgattgg agtcattctg atgtttgagt
     8821 ccgatacttt aatataaacc gcatcacgta tatcataagt aaatccgtct attttctctc
     8881 cgtcaggaag cgtgaaagca tagtttacaa cataggtgta cctgttgggt attgcttcgc
     8941 ctctttcgcc accctgcctt cgaatgccgg ttattacagc agtgtccttt tcaccggcaa
     9001 accccaggga aatccgcagc aagcccaagg caatcatcat aactgcaata agcattacta
     9061 ataaaggccc gattcttttt ttcagattag ttctgaacat acggctattc cccatttgca
     9121 ttgaatttgc ttaatataat gtcactaacc agatgaaagt tgtcctcatt acagaacaaa
     9181 gcgatttttt ccgcaaaacc cccaataaca actatagatc tcgatttggg atatactttt
     9241 acttttctta tgcttttcca tgctataaat accgattgcc ttgtttgagc cagcaatccg
     9301 gcacctgctg cagagggcct ttttgaaaac agcccgatga taatgagcaa tgagtttaca
     9361 ataaggtttt tcttcgcttg cttcctttga gtgtagttga ggattccgtt cttatcaatt
     9421 agataaccgg cagagtatct gcccccatat acaaccatga taaacagaaa acccgcaata
     9481 aataatagac cgattaaggt caggggatat tttattccgt cagcggaaac gtcgcctcct
     9541 gaagcaataa agagaatgag gataagcagc ccgaagggaa ttcccagcgc aattcccaga
     9601 tcctttaaaa tcgcattatt cttgaatatg ggcacatcaa tttcccaata gaatttggtt
     9661 tgtttatatt catccataat tatatactcc gtttatctag tataaattgg taatgcaata
     9721 acggcaataa ctgcagcaga tggcatacag taatacccat tttttcagcc ggccaattcc
     9781 ggaatgatgc ctgtctcacg gcggaatttc tcaatgatat cccggagttc aggttcatta
     9841 atattgctat gcagcagtct tatagtttta tacttttctc ccttaccata ggccaatagg
     9901 atataatcat gtccgacttc aacttttttc aggctgattc cttcggtatt gaatttaatt
     9961 atctttgaat caagcagaat tccaaaacag gatacaaaga tttcaataga cttattcatt
    10021 ttggattcgg attctgtttc agccgcttca atatgaatga ttttattttc tattatcagc
    10081 cttgcaaagg cataccttct gtattcattc agagagaata ccaagagcag gatagttgtc
    10141 agaatcgaga atacaacaac tatatggaaa tacccggcaa agagcgtaaa ggaaataaga
    10201 atcccaaaac tgctcagcat catataaatc ataagtttac gcttaattgt attcaaagcg
    10261 ctatccccct ttaggaataa agctcctccc ctttttacgg agaggagctg atattatcca
    10321 tactcaggat tattcttcgt atactatgac tgcttccttg gtgctgttaa tccagtccac
    10381 tttgcagttg aggttctctg ctgcaaatct taccggtaca aaggttcttc cgttcttaga
    10441 tacgggggct acatccattt tctggctgac tccgtttatt tttatatcag ttttgtccaa
    10501 ccacatttca accacatttc ctctggctgt tagtgttatc ttacgtgtgt cttgctccca
    10561 tcctatcgtg cctcccatgg cttccacgac ggcacggatt ggtaccattg ttcttcctgc
    10621 aatggtcata ggtgaggtgc tgcgtcccgg gtccacttcc tggattactc cgtccacact
    10681 catattcggg ttgtcaagct tgagcatgat gaagtgctta aagcttccgg gcttgtagac
    10741 ctggctgcct gttgttactg tatatgtggc aattgcagca cccagttttt catctattcc
    10801 ttcgaagggt tttgcttctg cgagtaccgg ctttacagtg taatagtaag tggtgttggc
    10861 ttctatattt acatcagcat aagaggtgcc ggttatataa aagtcagtta ctgatatgcc
    10921 aaggtcactt ttggaggttg acctgaacag cctgtatccc aatccgtcag caggctgcca
    10981 cattatcctt gatccggatt caaatgtttc tgcattgctt cctgccgaat tggcctgagt
    11041 tgttgtaatt acaggtgcag gagatgttgc tgccggtttt ggtgcgggaa taacctcagt
    11101 aatacgcatg ctgccatccc aattactcca gcttccttct gaaccgtagc gccatctgcc
    11161 ggtaaagctt ttcccgtcgg cggacattgt aagctccatg tcaccggcat cggacggtgg
    11221 tgaataggat ggggactcgg accaggtacc ggtaaagaca tttccggata ttgtgccgtt
    11281 gatttttccg ctgtcgtggg tataggtacc tgtaacagaa gcaccgtttt gggttattaa
    11341 cattttaccc cagttgctgt cccattcccc tgcccaggag taggtaccaa gatcggtagt
    11401 tcctgatgaa atgggagcag catttgctga tgaggtttgc ttccattcat aggtatacct
    11461 tacacctacc gaacccggtg agcctgcata aactcccaca tagagggaat gcttatctcc
    11521 ggggcggcct tctcctatga agttcagggt catatctgcc gtaaaggatt gttgtgcctg
    11581 cgcatcttga ttgtatccta ttcccggata ctttgccgtg gtaccgtcag cataggttat
    11641 tttagcaatt accttgctgg ctgtggcata tccgatatct atatcttcca tgtccatatg
    11701 gaagtaagga ttgaagccga tggagtaatt cccgttcctg ttgcttataa cattctgttc
    11761 aagctttaaa gtcactgtct ggtttggcat tatggtgctt ggaggattgc tccaggtata
    11821 tattgcgtgc atatctgtcg ggttgtcata gtcctttgat gcatatatac ctaagccgta
    11881 agcccatgca tactgcaatt ctatagtgtt gcctttaagg ctgccgtaaa agctatctgc
    11941 ttcaggtacc ggatattcgt gagtatccac cagcacccat gcatatcctt cctctgctgc
    12001 cgaaacaacc gttgtaaatc ccgcagccat actaaagagc agcgcaagga cggtgactat
    12061 tgatagaaac ttcttcataa tataactcct ttccgatatg taaatattaa gtattcatct
    12121 atcccaatat atctgatata ctcaaaaaag tcgtcacccg aaatgggtga ttacgttgat
    12181 tgaagtaaaa aatccctcat atataataat gagagatata gaatgcgtca ttaattgtat
    12241 tgcagattag tgggtttcct ttcccgtcag cagctttata agctccgttt tgctctgaat
    12301 gttgaatttg gagtagatgt tttttatatg tgtttttaca gtgttttcac tgagacaaag
    12361 ttcatcggca atcatcttgt aggttcggcc tttgagaagc agggctgcta tttcgctttc
    12421 ccgctcggta agccggccga taactgtaaa attctcaatt gcttttttct gcttcgatgg
    12481 ttcaatttcg ctcaaggccg tcagaaaaat atgatttttc aagaggccgg aaagatgcct
    12541 gtgcagcagc ggaagcagta tgaggactat aaatactacc acaagcgcca atacggatga
    12601 gtcaatgctt cctgtatccg aatctgcaat agtgcttcca aacatcccgc ctatcaatac
    12661 tccgaaaaca tttgcagaaa gcccgatgcc aaggattttt gccggattat ggtgataatc
    12721 cagcatttct cccagaatgc tccaccagaa caggtcatat actccgcagg cccccagcat
    12781 cagggtatct atgataagat agcttgcggc agaacggtca agtatcataa agcccacaga
    12841 tgaaaggcct atcattgcaa tggcaacgta gagtatgtag cttctgttgg ccttgccgga
    12901 aaggtttctg acaatatata aggctgctat atagggtaag gcccaatacc agctgactag
    12961 ccactcatga tgggcaaagg cgggattgat gacctgatac atcaagccgg aattaatggt
    13021 gattatgacg ataaacaagc ataaaaaggc caaaggcttt ataggactga cagcctttat
    13081 aggctgtctg gtatgccttt cttcagctgt attctgatcc acagccaatt tagtatgaaa
    13141 aaacaatgat attgaaagca tgagtatgga cagtgcaagc cccaaataag aagatataag
    13201 gacggcagca gtatttataa taaccatgag gatatttgaa tagattagga catcggctat
    13261 gattttaatt cgttcatagg agaaagcgag cattttgaag tagaagcccc aggctgccac
    13321 gcaagcaccg gacaggaatg acaatatcag aagcgatata tgccacatta ttgaaggaat
    13381 aaagaaaaaa actacagtac cgagtataca aacaggaatg gaaaaaagca ttagaccctt
    13441 tgcggctttt gttgatttta taaaaaatcc gcaggcaaac agaccgataa aatgagctgc
    13501 aattgctccg aaggtcagct catttacgtt aacattatta atacttgcaa ttgtaagcag
    13561 tacctgtccc ccgaagagga aggacaggag ccatgaagaa aaggatgaaa aggcaataat
    13621 tgttttactg cgctcatctc tcaacaaaaa tggtaccccc ggtctttata taaattaatg
    13681 ctttatggat taattatact ttgaaatgtg cattattcca agagcagcat cagaaaatta
    13741 tatagaacac aggctgaaat tttttgctgt gttctatatg gatttttatt tcctattttg
    13801 aattcttgtt tttattatta ataatctcct catgattcat ataagaattt accagctcct
    13861 cagcctgggc ttcttcattg tccggattat aattttcggg gaaccagtag gagttatatt
    13921 tacgttctat tatgggatac ttttccccgt tgtacgtctc atctattata ttcatggctt
    13981 cacctcatcc ttatccttct ctttaatgtc ttcattctca agggcaatat tttctatccc
    14041 gttatatttt ttatgtgaat catgatttat tttggccatg actatttcat agtccgtaat
    14101 tggttcctcc gcagcaggag tcaggacgtt caatttttta tttatttttg ttgacataaa
    14161 atttcacctc gttttatttt tagctatttt tatttttgta ttcattgaaa aattgcaaag
    14221 cgtcattgaa acgttgcggt tgttcttctc tttatcagaa ggatgcttta taatagtatg
    14281 tatcaaaaga aaactaccgg aggtactgat gaattatttg gagaacctga acaaagagca
    14341 gcgggaagca gtgctgcaca ccgaagggcc gcttctgata cttgcggggg caggcagcgg
    14401 caagacaaga gtacttacct acaggatagc ctatttgata gatgaaaagg gcatatatcc
    14461 ttcgaatata cttgcaataa ccttcaccaa caaagcggcc agggaaatga aggagagagt
    14521 tcacagactt ataggcaata ccgataacat gtggatttcc acctttcatt cggcttgcgt
    14581 cagaatgctg agaaaaaatg tagaaagcct caagggttat aagaagaact ttgtgatata
    14641 tgatacgaag gaccaggaaa ctctgataaa ggaatgcctc aaggaactga atctcaatga
    14701 gaagaatttt cccttcaggg ttgtatctgc cgaaatatcc gcagcgaagg acaggctgat
    14761 acccccccgg gagttctatg ataagaacat gcatgatatc agaagacgga agcttgcgga
    14821 tatatacatg ctgtaccaga aaaagctgga gaaaaacaat gccctggatt ttgacgatat
    14881 aatcagcaag acggtggagc ttttaaagct aaatccggat atattggaat attatcagaa
    14941 caagttcaga tacattttgg tggatgaata ccaggacaca aacttcagcc agtatacctt
    15001 tatcaggctt cttgcggcgc agcacaggaa tatctgcgtt gtaggggacg atgaccaggg
    15061 catatacagc ttcaggggag ccgatatcgg caatatactg aactttgaga aggatttccc
    15121 cggagctaaa gtggtcaagc tggagcaaaa ctaccgatcc accgaaacaa tactggaagc
    15181 tgccaatgcg gtaataagca acaattacgg cagaaagagc aagaagctgt ggaccgacaa
    15241 gggcacaggc cgttccataa tatatcacta tgctgtggat gaaagggaag aggcaaactt
    15301 cataatggac gaggttgaaa ggatgatagc ccaggaaaac cgaaaactgg gggaatttgc
    15361 gatattgtac cgtaccaatg cccagtcccg tgtacttgag gaaacttgta tgtcccacgg
    15421 actgccctat agaatagtag ggggcttcaa gttctatgac aggaaggaag taaaggatat
    15481 aatcgcttat ctgagagtca ttcagaatcc cgatgaggat ttgagcctga aaagaataat
    15541 aaatgtcccc aaaagaggca tagggaattc aactctggaa gcgatagccc aatatgcccg
    15601 gactacggga gattccatat tcggtgccct gcttgaggtt gacaggattg aaggagtaag
    15661 cacaaaggca aaaaagggaa tcaaggaatt tgttaagatt atgagcgatc ttatgggcat
    15721 agcggagacc gaaatggtat caataattgt aaaggaagtg ctggaacgca cgggatatct
    15781 tgaccagctt gaaaaggacg gagatgaaga atccctaatg agagcggaga atatcaagga
    15841 gctgttatcg gcgacctttg agtttgagga attgaatgaa agtgccttat tgcctgagtt
    15901 tcttgaacaa atggccctta tgtccgacat agatgcggtg gaggaaggca aagacgcttt
    15961 gataatgatg accttgcaca gtgcaaaagg ccttgaattt cccttcgtat tcatatcggg
    16021 catggaagag ggggtatttc ccagccagag gtcatacttc gaagacaatc agatggagga
    16081 ggaaaggcgg ctgatgtatg taggtataac ccgggctaag gaaaggcttt acctgacagc
    16141 cgcatttgaa cgcacattat atggaaatac tacctttaat acaatatcaa aatttgtacg
    16201 ggagattccg aaagaattgt tagtaaaagt ttaagttttt agtttgtata tttattgcaa
    16261 cgcagccgga gcttcaaagt cagatacttc taaactcgtt ccgggctgca tcaaaactcg
    16321 cttagctcaa acagttgatg cagctgaccc tccacttcgt taagaagttc tagtgctttt
    16381 acgcaaggct gcttttacaa taaacataca aattcaaaac atataatctt aattcctgat
    16441 gtaattctaa aataatagta tatcaaaccg aagggaggaa tccgattggg tatagataaa
    16501 ctggaaaaaa gggctgatga gcttattaag ctcataaata ggcataatta caactactac
    16561 gtgcttgaca agccggaaat cagcgattat gaatatgatt tgctcatgaa ggagcttata
    16621 gagctggaaa acacattccc ggctttgaag cgggccgatt ctcccaccca gagagtaggg
    16681 ggagaggtcc ttaaggggtt tgcggaagtc gtgcacaaga ccccgaagct gagcctgagc
    16741 aatgtatttg atgaaaacga cataagggat tttgattaca gggtaagaaa ggccgtagga
    16801 ggcgacgtgg aatatgttgt cgaattgaag atagacgggc tcacagtggt attgaactac
    16861 gaggaaggcc ggtttgttct gggggcaacg agaggtgacg gaataaaggg cgaggatatt
    16921 acttcaaatc taaagacggt aaaatccata cccctgctgc ttgaagagga ggttgacctg
    16981 gaggtcaggg gagaagtttt catgtccaaa aaagcctttg aagagctgaa tgcaaggcgt
    17041 gaagagcagg aggagccttt gtttgcaaat ccgagaaatg cgtcggcggg ctctctgagg
    17101 cagctggata ccaaagtgac tgcaggcagg cccctggata tatttatatt caatcttgaa
    17161 agcatagagg gaaaaaactt tgatacccat gtggaaacct tggaatacct taaggaattg
    17221 ggcttcaagg ttagcccctt catcagcgtg cacaaaagca gcgaggaaat aatagaaaag
    17281 tgcaaatact gggctgacaa aagaggagag ctgccctttg taattgacgg ccttgttata
    17341 aaggtaaaca acctcaggca aagagaagcc atgggatata cgacaaaaaa cccccggtgg
    17401 gcgacagcat ataagttccc cccggagacc aaaaagacca aagttaagga tattatagtc
    17461 caggtgggaa ggacgggagc cataacccct actgccgagc tggagcctgt aaggcttgca
    17521 gggtctgtta tcagcagggc aacccttcat aatgaggatt acataaagga aaaggatata
    17581 agagtcggag atacggtatt aatcaaaaaa gccggggatg tgatcccgga ggtcgtggaa
    17641 gtggtcaggg aggacaggga cggatcccag cagcccttcg aaatgccgaa aaaatgcccc
    17701 gtttgccagt cagacaccat tagggagcag ggagaggcag tgaccaagtg tacaaacatt
    17761 tcatgccccg cccagcttaa aagggcatta tttcatttcg tatcaaggga tgcaatgaac
    17821 atagaagggt tcgggcccca gataatgagc ctgctgatgg ataaagactt tatcagggat
    17881 gcggccgata tatacctgtt aaagaatcac agggatgaac ttgttgaaat agaaagaatg
    17941 ggtgaaaaat cagtacagaa ccttttggat gcaattgaag cttccaaggt aaacccgctg
    18001 aacaggctta tctatgccct cggaataagg atgatcggac agagggcgtc gcaggttctg
    18061 gcaaatgaat ttgaagatat cgaagccatg tttgatgcgg actatgaaag attgaccgga
    18121 atatccgaaa taggggataa aatggctgaa agcataataa cttttttcaa gcaggagcag
    18181 aatataagat taataagaaa gcttcaggaa tgcggtgtga atacaaaggg atcaaaaaag
    18241 gaaataaagg aaaatgaaca tttcaaaggc aaaacctttg tacttacagg agggcttgca
    18301 aactttacca gggaacaggc gaaggaaata atagaaagct ttgggggaaa agtatcggga
    18361 agtgtaagta ataagacaga ttatgtactg gcaggtgaaa atgcgggttc aaagcttgac
    18421 aaggcaaatg aactcaatgt ggctgttatt gatgaggata ccttcagaag ctgggctgaa
    18481 atatagaaga ttaatacaat taaagttcat aaaaaaatca acaaaagttc acaaaaaatt
    18541 cacaatactc tggtatatta atatttaaga tgtatatcaa atatacatcg attagcaggt
    18601 tttaggggta tatcttacaa cagcataatc aagacagtgt ttcatctctg ttaagtcatg
    18661 gcaacggatg aaacagggtt tcaaagatta gttgttatat tagattacta ttgaatttga
    18721 acacctgctg atttttttaa acaattatag gacatttcaa gcatatcgaa gtatttttac
    18781 tatttgacgc cggaataacg gcaagaaggt ttgcattact aatattttat gttcataaaa
    18841 tattttcatt gttgatttaa tatttttgtt aggctattaa tttacataga tgcctaaaac
    18901 taaaataaag ggggatgaat tatgaaaaag tctttagttt tagtactggc aatgctgatg
    18961 atcttgagct ttgcattatc agcatgcgca ccgaaggctc ctgcagaaac tcccggagca
    19021 gaaactcctg caccggctgc agaaccaaaa atattcagaa ccaacaatat gagcgagccc
    19081 ggctcactgg atccggctct ggcacaagga actcatgaat cttggatact tgaccatgca
    19141 tttgaaggcc ttatggcaag agatccaagc ggtatatctg taccgggtat ggcagaaagc
    19201 tatactttag cagatgatga ggttacttat acattcaagc ttcgcgatgg aatcacctgg
    19261 tcaaatggtg accctgttac cgcatatgat tttgaatttg catggaaacg tgcattggat
    19321 ccggatttgg cagcagatta tgcattccaa ctctattatc ttaaaggcgg ggaagcttac
    19381 aatacaggtg caggctctgt caatgacgta ggagtaaaag ctattgatga taaaacttta
    19441 gaagttgttc ttgcaacacc tactgcttac ttcttggagt taacatcctt ctatacttac
    19501 tatccggtta acaagaaggt tgtagaagct aacgccgatt gggcaaaaag tgctgatact
    19561 catgtgtcaa acggaccttt caaattaaca ggatgggaac acaatgctac aattacgttg
    19621 gctaaaagtg aaaactacta caataaagaa gccattaagt tagatggtat tgagcttgca
    19681 attattgcag atgaaaatac cgcttggcag aaatatttag gcggagaatt tgatttcctt
    19741 actcctctgc cacaagcagt tactgcacag ttaaaagctg aaggcaatcc tgaactggta
    19801 atcggcggag aagtcggaac atattattat aaccttaata ataaattaaa accctttgac
    19861 aatgctaaaa taagaaaagc tttgtccttg actttggaca gacagacaat ttgcgataag
    19921 attgcccagg gtggacagct tccggcagag ggagttgttc ctttcggaat gcttgatgaa
    19981 aacaacaagg aatacaggga cggcgcaggt aaattagtag agtataatgt ggaagaagga
    20041 aagaaactgt ttgccgaagg tctggctgaa acaggcttca aggttgaaga tttcagcaaa
    20101 ttcacccttc tttacaatac ctctgaagct cataagataa tagctcaggc agctcaggaa
    20161 atgtggagaa caaatcttgg tatcgaactt acactggaaa acgttgactt ccaggttaag
    20221 cttgacagag aaaaagccgg agattttcag atttcacgtg ccggatggat aggcgactat
    20281 attgacccga tgactttcat ggacctgtgg ttatccgaag gtgcctttaa cgatgcaaaa
    20341 tacaacaatc cggaatatga cagattgatt aaagaatcaa aagcaactac agatcagaag
    20401 gttcgtatgg aaaacatgcg taaagcagag cagatgctaa tggaagctat gccggtagtt
    20461 ccggtatact tctatactca gccatatgct cagaaaccat atgtaaccgg cgtatttaaa
    20521 ccgcttaaca ggtatcctat attcacattt gcagatatgg atatgagcaa aagataaaat
    20581 aatctaaatt tttatagaag atcgatgggc tgtttatata cagccctcgg tgttcttttt
    20641 attaatggca aagattaatg gtaaaggagg taaagaatga aaaagtattt aataaaaagg
    20701 gttctgatgt ctgttgttac aatttgggcg atcattacca taacctttgt attgatgcat
    20761 tctgttccgg gtaatccttt cagacaagaa aataagatgc ctaaggtagt atacgaaaat
    20821 cttttgaaaa aatatggatt ggacaaacca ttaagtgaac agtacttcat atatttaggg
    20881 aagatagtta agggcgattt tggtgattct atgaaatcaa gggttgaaac agtaaatgat
    20941 atgattagaa ggggcttccc ggtatctgcg gtaataggtg ctgaggcctt attgatagct
    21001 ataatattcg gcccggcatt aggggcgatt gccgccttga atcagaacag gtggccggac
    21061 tatttatcta tgataattgc tattatcggt atttctgtac caagttttat aatgggtaca
    21121 gtatttatac aatttctggc ccgaaatata gaatggctgc ctattggagg atggggaagt
    21181 ttcaggcatt caattttacc atctcttgcc ttagccctta tgcccttggc tcagcttgcc
    21241 aggtttatgc gctcaagtat gctggaggtt atgggacagg attatataaa tactgccaaa
    21301 tccaaaggaa tatccaaagc tacggttgta ttaaggcatg ctgtaagaaa tgccatactc
    21361 cctgttgtct cagttttagg taccgttgca accaaccttt tggtcggtag ctttgttata
    21421 gaaaaaatat ttggtatccc gggtctcgga atgttcttta ttatttcgat caataatagg
    21481 gattaccctt tgattatggg aactacaatc ttttatgcca ttattttaat tgttatgtta
    21541 ttgattgtgg acattgctta tatgttcatt gatccaagaa taaagcttgc taaggagggg
    21601 cgatgatgat tactgaattt gaatattcac ctgagctatt tgaattagca tcaaaagaag
    21661 atagaaatgc agaaaaaata cgtcgtccca gcataaaata ttggccggac gtatggcgca
    21721 gactgaaatt aaataaatta gccatggtag gtttaagtct tatagttttt tttggtatta
    21781 tggcgtttgt gggtactcaa attaatggat atacatattt tgaacaaaac tattcattga
    21841 taaatgtgca ccctgacagc gtccactggt ttggaactga tgagttagga agagatttgt
    21901 tcacaagaac ctggtttggt gtcagatatt ccttattcat aggagttgcg gcagctttta
    21961 ttgatttctc cataggagta ttgtatggag gtattgcagg tattactacc agaagaatag
    22021 atgctgtaat gatgcgtatt gcagaggtta tatacagtat tccctactta ttagtggtaa
    22081 tccttatatc agttgtgttt tctgcagccg gccaaggaag ttcaatgttt gttttgattt
    22141 tggcactgtc tttgacaagc tgggtaccta tggctatact cgtccgtggg caagtgcttc
    22201 aattgaaaga aagcgagtat tcattggctt cagaatccct gggggctgat aagaaatgga
    22261 tattgaggaa gcatattata ccaaacactc ttggaccaat attggttaat attactttat
    22321 caatacccag agctattttt gcagaagcta ccttgggctt tataggtctc ggacttcaag
    22381 cacctaaatc aagcttggga actttggcta acagcggatt agcgggcctt ccggtgggga
    22441 attcttatca attattcata ccggcgatat ttatttcact gattatgttt gcttttaatg
    22501 tcttaggcga tggacttcgt gatgctttgg acccaagact gcgaaaatag agagaggtgg
    22561 aaatatggaa aatgtactag aagtaaaaaa tttaaagata tccttccaga ccttttttgg
    22621 cgaagttgaa gctgttcgag gtatcacgtt cagtgtaggc aagaaagaaa ccttagctat
    22681 tgttggagaa tcaggctgtg ggaagtctgt aacagcaaat ggtattatgc agcttctgcc
    22741 taagcctccg gcatttttca agggaggaga aatactattc aacggagaag accttttgaa
    22801 gaaaacggaa aagcaaatgc aggcaataag aggaaataaa atatcaatgg tattccagga
    22861 ccccatgacc tctttgaatc caacaatgaa aatcggatat cagatagttg aaggcttagt
    22921 gaagcataat aagatgagtc gtgaagaagc taaaaagaaa tctattgaaa tgcttgaact
    22981 ggtagcagta cctcagccgg aaaaaagaat aaaccaatat cctcatgagt tttccggagg
    23041 tatgcgccag agagtaatga ttgcattggc tatggtgtgc aatcctcaat tgctgattgc
    23101 cgatgagcct accacagctt tggatgttac ggttcaggcc cagattctgg aactgatgaa
    23161 gaagcttcag gatcagatgg ggatgtcaat aatccttatt acacacgatt tgggaatagt
    23221 ggccgacatg agcgacaggg tcgttgttat gtatgcgggg cagatattgg aggtaggttt
    23281 gactgatgag atatttgctg cgccgaagca tccctacaca agaaaattgt tagcctcggt
    23341 acctcgtttg gatatgagca gggaggaacc gctttattca atagacggaa caccgccgga
    23401 tttgtatgtt ccgcccaagg gctgttcttt ctatgaccgc tgcagtgagg ccatgaagat
    23461 ttgcaagaat aatatgccgg aatttgatga gcacagtgca actcatcaaa gccgctgctg
    23521 gttgaaccat cccatggcac aaaaaacaaa ttcaacggat gggagggaag tatgatgaca
    23581 gaacctttaa tctctgcaag aaatataaaa aagtattttg atgtgaaggg cggaaagcta
    23641 aaagctgtgg atggcatcag ctttgatatt tttccccggg agacctttgg tttggttgga
    23701 gagtccggtt gcggaaaatc aactgccggc aggactatag tgcgtctgta cgatccaacg
    23761 gagggagagc ttatattcaa cggacagaac atatataagc tttcaagaaa ggatatgcag
    23821 gagatcagaa gggattttca gatgatattc caggatccct atgcttccct taatccgaga
    23881 atgacagttg aagatattgt agctgaacct cttgacatac atcagaccta taagaataga
    23941 gcagaacgta ccgaaagagt aatagagctt ttaagacttg ttggtttaaa tgacgagcat
    24001 gccatgcgtt ttcctcatga attctcagga gggcagcgtc aaagaatagg catagcaagg
    24061 gcattggctc tgaatccgaa atttatcgtc tgtgatgagc ctatttccgc attggacgtt
    24121 tcaatacaag cccaggtggt taatcttttg aaagaactgc aggacgggct gggcctgact
    24181 tatatgttca tagctcacga tttgtcaatg gtcagatata tatccgacag ggttgcggtt
    24241 atgtatcttg ggcatatgat ggagcttgca gagtcggatg aactgtactc caatcctgtt
    24301 catccctaca ccaaggcttt gctgtcggct atacccatac cgaatcctga aatacaaagg
    24361 aacaagaaaa ggataatgct ggagggggac gtaccaagcc cgataaaccc cagagaagga
    24421 tgcaggtttg ttgacaggtg ccaatatgcc ataggacgat gccgggaaga aacacccgta
    24481 tttgaagaga aggcagcaaa tcattttgcg gcatgccatg ttgtatagtt gataattgga
    24541 taatggcgct gtcgaatatt gcccgggcaa tattcgacag cgccattact cattattcac
    24601 atagtttttt cagatttgtg aaaactaata ccacaaatta tttaggagag caatgccatg
    24661 acccttgaag attatttgga ggaaggctgt gtatatcatg tggtcagtat tattgacttg
    24721 aaaaagaccc tgcatgaagg gatcagatac gatgataaga atacttatgc ctgtaaatat
    24781 actgacttcc atagcttttt cgacctctac aagcccaaag gaattccgga ctgggtagag
    24841 aggaggaagg ccatatttgc cagtatcggg ttcaaggaag gtcataaatg gcactctcac
    24901 agcgccatat tgaggataag gattttaaaa gacagatgct ggatatgtaa tgaaaatctg
    24961 gcaaatttca tatatgagcc ctttatactt cagcacatgg atggattcga gggcacaaaa
    25021 gaattcatga aggctaacgg aaaagcattt gcggggaaat attggcagaa ctcattgagc
    25081 ttcaacgata accttatcaa aaggtatgac aaaagggaag gctacgatgc cgaagtactg
    25141 gtaatgcatc ccatacctcc ccggaatatt gaatgcctct atatagtatc agatcaccgg
    25201 ataatgagct atcaggcgtg gaaggaatat ttcacaccgg gaagccctca tgctgaagtt
    25261 caatacaaca gatatttgca gcctgacaat atgtcttcgg cctggaagta ccccggctca
    25321 caatgatatt ttcttttatt cagcttgact tcaaaatact cagaaaagac aggagctgag
    25381 cgtttttccg tggagcccag gttatgaagc tccagcttgg tatcctgcag tatagggtag
    25441 aattggttgt tgaacagctt gctgcttata aatgttgcac tgtaaaatgc ggatagctgt
    25501 ctgtccacat gcatgaaaag gccggcgtcg gtaagacagc tgcgaagcat gcacaaagcg
    25561 gcaaaaacgg ctgccgtctc cttaaagctg tatttttgct tgttttttct cgtgaccagg
    25621 aataatccgc tgcttctgtc ccaatatgaa tcaatccggt catagagctt aaggaatgaa
    25681 gcctcatata caatcaagtc attgacctca tgcagcttta tcaggaaatt catacagttg
    25741 cacaaggtta tgaaggagct gaagctatca tcggaaatat ttcttgcaat atcgccggta
    25801 tgggccaaac gctcgcaaag ctccatgcct attacattgg ctgtgctgta tactgtttct
    25861 ttgtacacgg cggtatgccc cagaatggac aaaaggcaca agcccacgac tgacagatcc
    25921 cgtgagctga tatcgtttat attttctgta atattctcac ataataccgg cagcacttcc
    25981 aaagccttct taagtctggg tactaaatct cttgaatatg aatcatcccg atcaatcagc
    26041 cccaatatcg aagaaagggc ttccgcaaca aaaaattgag ttttgatatc cggattttcg
    26101 ttgttaatgg taattctata ttcaccatag acgttatccc ccgtatcctc tccggaataa
    26161 taaaagtctc caagcttaag atagtctatg aggaagcggg cctgtttaat tgagtttaca
    26221 ccgaggcagt aggcaataaa ttccttattc tccatttgta tttccccatt ttgtataata
    26281 ggtatgaggg cagtcatgct gcatatggaa tgggccagta cattgggttc aacggatttc
    26341 ttcatctttt tctccagcca gcggaagctt ttgaagtcat aggaagcttc cggaatggct
    26401 gcatatatgg gaatgcacct tttataaagg gaagacataa aagtaatatt tttaggcatt
    26461 ctgatgctgc ctgccccttt tagaagcttg tatatatgac ttaactcctg ttcatcaggc
    26521 atctctatac ttccgcagcc cgaaagcagg cagaaggata ttacgttgag atatgtcatt
    26581 gtgttgcatt gttttattat gtcaggatat gagaattcaa agatcattga aacaccaccc
    26641 gataaaaaat attgcaattg gtatatctat atatgtttat gcagtaaaag ggatggataa
    26701 aactaaaagc tggtacttta ataataggca taagatacat agacggataa cataaattct
    26761 tgcgaattta tgctataatt aaactatata ttgactgctg aagaaggtgt tgaatgtgaa
    26821 ggaagggaag attcctccgg aactattgaa aagcttggta ttcaataata tcaaggtacg
    26881 aaatgaagaa gtaatattgc ggcccgaaat cggagaggac tgtgctgcca tagacttcgg
    26941 aagccatgca tgcgttttgt ccaccgatcc gataacagga gccgtcaaga atgccggtgc
    27001 cctggcaata catatatcct gcaatgatgt agcagccagc ggggtaaggc cggttggaat
    27061 aatgataacg attctggcgc ctccggaagc ggcagaaggg gatataagga gggtaatgga
    27121 ggaggcggga gaagcagcct caaagctggg agtcgaaata atcggagggc ataccgaagt
    27181 aacctctgcg gtaaaccgta ttgttatatc cacaactgcc ttagggaagg tgcggaagga
    27241 cagaatagta aggtcttcgg gagcaaaggc aggggatgat gtgattatga ccaaatgggc
    27301 aggcttggag ggcacatcaa taatagcctc ggataaggga aaggagcttg aagccgttct
    27361 gacaccggag gagatagagt ctgccaggtc ttttatcgat aatatcagtg tagttgaaga
    27421 aggagtgact gcgggggaat tcggtgccaa cagcatgcat gatgttacgg aggggggaat
    27481 attaggggct gcctgggaga ttgcagaaag ctccggaaag ggtatagaca tatatattga
    27541 caagatacct gtctgggagg ttacaaaaaa aatctgcagt ttttatggaa ttgaccccta
    27601 tagactgata tcaagcggct caatgcttat taccgcatca aagggagccg aactggcaaa
    27661 gctgcttgag aagaagagaa taccggctgc aataatcgga aggataaccg aggccggcag
    27721 aaatataata agaaatggga atatttctct tttgaaccct cctgatacgg atgaaattta
    27781 taaagcttta aaataaaccg gaacaaaaca tgtaaattcc cgtcgaaaga tacgagcata
    27841 tcgtatcttt tttctttgcg ggacatgggg ggacacgggg acgtttctcc tgtcccacct
    27901 tcagtgggac aggagaaacg tccccgtgtc cccggagaaa cgtccctgta tcccccgaaa
    27961 cgtccccatg tctccttgct ttgaaacaga aatgaaacat tatggtaaca gaattataat
    28021 attaatgtaa aggtatttgg ggaatatggt cgaaatatat agtagaatac gtctattttg
    28081 acattgtata agatattccg caaatcttgc tgaattgcgc atggatagga tatctgaaaa
    28141 ataatctgcg aggaggaatt atatgaaaaa agtatttgta gttataactg tgctctgcct
    28201 attattgaca agctttgcag gcaccgtatc cgcacaagca tctgaagcaa aagcacctcc
    28261 gctgtcaatg cagatactgg gaaagaaagt aagcacagca agccctccta ttgtaaagaa
    28321 caacagaata tttcttcctg taagggcggt aggggaagca ttaggctaca aagccacttg
    28381 gaacgcccaa accagtacca tggagcttaa ggactccaag cagacattga agataaaagt
    28441 aggcagtaca gatgcttatg tcaacggaaa taaggtaaag ctggatgccg ctcccataat
    28501 gtataaggaa aggtcataca tgccgttgac ttttgtgcag aagcatttca actataccac
    28561 tacatatgac aaaaccaaga acataatctc aattgacaag aaaacagaag cggacaaaac
    28621 agcagcacag acccaaggct tgtatgtact ggggaaaaag ataggaacgg atgccccggt
    28681 aataaagaac aacgctgttt acataccctt gaggcatgcg ggggaaggca tgggctataa
    28741 agtcagttgg tatgccggcc cggagactat ggaaatgaga ttgggcgaca acttggtcaa
    28801 ggtaacggta ggctacggct tcggtgttgt aaacggcaaa aatgtcaaga taagtccggt
    28861 acccattatg tataattcac ggttatatgt gcctataacc tttattcaga acaacttcga
    28921 ctatgatgtt tcttatgaca aagcaaagaa cgctgcaagc atagataaga agaaaccgcc
    28981 tgcagcagaa gcggaagagc ctatagaata caaaaaggcg aaaatagaag aaataatcta
    29041 cgatgataat gggggatttc cccagttgaa tatagttgca gacaaccccg ttaagtacaa
    29101 aagctttacc atggtgaatc cggacagaat ggtagtggat atagaaaacg ccgttctgaa
    29161 taccgagttt gaaacaaagg aaataggaaa aggcggagtt attagagcca gagtggcaca
    29221 gttcagcaaa tctcccgatg tggttagagt ggtaatagac ctggagaacc agcagttgtg
    29281 caagctggtg caatccaatg acaagaagac aatatccctg atatatgcaa acataataat
    29341 tcctgtcagc tttgtcagag aagaaggcag agatgtgctg atgataaggg gaagccagga
    29401 aatggatacc aatgtattca cactggaaaa ccccgacagg ctggtagtgg atataagaaa
    29461 gtctgtgctg agcgaaaagg ggcagacggt tgaaaccaat acgccttttg tgaaaaccat
    29521 aaggacaggc caggtggatg tgggagttgc acgggtagta cttgatgtgg aaccggggat
    29581 atattatgac gtcactaaca atgggagcct gacaaaggta tatatttccg acgtgccttt
    29641 ctctttcttt gattataata aatactacaa ttcggcttat gtagatctaa gccccggtgc
    29701 ggaagtggag tatcagccga taatagataa agcaaacaga acacttaaga taatgatacc
    29761 aaaggatatt gacgtggaag aaaaggtgca tgatattaat gacaatgtat tggaaaatgt
    29821 aacaattacc aaggaaactt acggagatca aatattcact gcagcagctt tcaaaatgaa
    29881 gccctctgtg gaatatgaac tgctttctcc cgcagttacc aagctcgtaa gcctgaagtt
    29941 caaatatagg gctttaaaat ccggacagct tactgtactt attgaccccg gacacggagg
    30001 gaaggacccg ggagcaacag ctattgataa aaagacttat gaaaagggcc tcaatctgga
    30061 tgtcgcactg agattgaaca gaatactgaa gggcataggc ttcaaaacaa ttatgacaag
    30121 aaccgatgac agctatgtgg atcttgccag cagatcggga cttgcaaaca acagctatgt
    30181 tgactttttc atgagtatcc actttaatgc cttcaacagc accgccaagg gtatagaaac
    30241 attatatttc cctaatgaag taactgatga ataccagtta gacaacagag aaatagctaa
    30301 tatattccac agcgagctta tcgatgcatt aaacagaacc tccaggggaa taacaccgag
    30361 accgggactt catgtattga acaagacaaa gatgcctgcc atacttgcgg aactgggatt
    30421 cctaactaat cgtgaggact ttgcggagat taagaaagat gagtacaggg aaatggcggc
    30481 acgggcattg gcagtttcaa ttgtgaaata ctttcaggat attgagaata tcaaccttgg
    30541 catagatact aatgccatat atgccgctga tgcgccaaag gatccgtaca gcttggagca
    30601 ggatttggcg tcaaacaatt gatttaaagc agtgaaacag agtgagacat ggggacgggg
    30661 acactgggac acggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc
    30721 cgtgtcccag tgtccccgtc gcattattcc acttatacga atatacccct tatgaggcta
    30781 atataatata ttgataaaaa aataaggggt gaaataaatg aacaggaatc ttgcactatt
    30841 attggcatta cttatatgct gtacattggt ccttacaagc tgcagtaacc cgctgatttc
    30901 tttatgggat ttctttaaca aaaagactga tacggtagag gaaagtccgc agcaagttca
    30961 ggaggaaacc caaagcatga gtgaagtcag tgtttcccaa ccggaagaca gcagagcaac
    31021 ggtactttac tacaaagaca acgaagactt tcttatacct gttatgcgat atatgcccaa
    31081 gggggatctt ggcgtagcaa aaacagcaat atcagctatc atttacagtc cggaaacggt
    31141 agaggatctc aaagccgcag gattgacacc tacactgcct atgggtacca aaatcaacgg
    31201 agcagtcatc cgggaaaatg gtttggctat aatagatttt tcaaaggaat ttctgaactt
    31261 cagcagcaaa aaggctgaag aactgggaat caaagcagtg acatatacgc ttacagagtt
    31321 tccaaacata aaatcagtgg aaatcagggt ggatggcaaa aaaattgaag aaatgccaaa
    31381 gggaactgaa gtagggtctg aacttaaaag aacggaaata aatcttcagc ctgtcgaaaa
    31441 ggcaggtgaa aagttatcaa aggttgtggt atactatcat aaaaaaggaa gcggggcata
    31501 ttcatatttt gttccattaa caaaagtggt atccggcttt gaaaacagtg tggaagccgc
    31561 cttaagcgca ctgctggaag gcccggaggc cggcagcctg aaaaatcctt tcccggaggg
    31621 tactaaactg ctggatgtgc aggtaaacag cggtatagct tatgtgaatt tttccgagca
    31681 catactggct aatcagggag acaaggcggc ggagaatgct gtgaaaaagg cggtagcgct
    31741 tacgcttaag gaatttcctg cgatttcaaa agccaagata tttgtaaatg gcaagatcgt
    31801 tgaaaatact gctggtgtgg gtgcggacga atatatatcg gttccggttt ttgttaattt
    31861 ttatgaataa gaattacata aaacaagcga tatggggatg tttcaaaatg tccctgcata
    31921 tcgcaaaaaa agctgacaag ctgtatgttt ttatggatgt cttgggaaga aagggaaaaa
    31981 taaatctgcc gtgctcatgg aataatataa taacagaact tttgaatcaa ggcagaaaat
    32041 ttatgcaact ctgtaaggga ttactaaaca caaaacaata cttttgaagg gaaggaagac
    32101 agatgcagag aattggcggg agaaacaacg aacaaataag gcctgtaaaa ataataagga
    32161 attacttaaa gcatgcggaa ggctcagtgc ttatagagat gggtgatacc aaagttattt
    32221 gcacggcttc agtagacgac aaggtgcctc cttttataaa aggaacaggc aagggctgga
    32281 ttaccagcga gtacggaatg cttcccagat caaccgaagt acgcaagcca agggagtctg
    32341 caagaggaag aatagacggc aggacaatgg aaatacagag actgatcgga agggcgctgc
    32401 gatccatcgt gaatcttgaa aaactgggag agataacgat ttatattgac tgtgacgtta
    32461 tacaggctga tggcggtaca agaacagcat ccatcaccgg atcctacgta gcccttgttg
    32521 atgctttata taagctggtt cagcaggaaa ggttaaaaga aatgcccttg aatggttata
    32581 ttgcggcagt aagcgttggc ataaaggacg gagaggcaat actggatctg aactattccg
    32641 aggattcaac ctgccaggta gatatgaacc tggtaatgac cgataggggg gaatttatag
    32701 aaattcaagg tacgggagaa gaaagcccct tcagcaaagt gcagcttcaa gaaatgatgg
    32761 agcttgccga gaaaggcata cgggagctga tagagataca aaagcgatcc cttggtgaaa
    32821 tcgccgataa ggtaggtaca tatgatgaaa atgatagcag cgacaaataa tagtcataag
    32881 cttgaggaaa taagagcaat attaaaggat aagggaatag aagtaatatc cctggcggat
    32941 gccggattgg atattgagat tgaagaaaac ggggattcct tcatgggaaa tgccctgata
    33001 aaggcgagag aggtctttcg ccttacagga gaggcagcga tagctgatga ttcaggcctt
    33061 gaggtagatg cactgaaggg acagccggga gtgaaatcag caagatactc aggggaatcg
    33121 ggagcggaca aggataagaa aaacaatgaa aagcttaagg aagctctgaa agacataccg
    33181 gataatgaga gaggagcaag gttctgttcc gcaattgccg cagtattccc ccggggaaaa
    33241 gagataactg cagaaggcta tgtatacgga aaaatcggtt atgaagaaaa gggagagcac
    33301 ggattcggat atgatccgct ctttattgtt gacggttatg gcagaaccat ggccgagctt
    33361 gactcggaag aaaagaataa aataagccac agggcaaatg ctctcaaaga gtttgtaaag
    33421 aagctggata tgctataaat tgcatggcaa atatacttcc aaatcagttg acacccaata
    33481 taaattttgt tataatctat aattgtcggc gatactaaaa acagtatatt gcaagcgtgc
    33541 gggtgtagtt caatggtaga acgtcagcct tccaagctga tcgcgagggt tcgattccct
    33601 tcacccgctc caaacatgcg cctgtagctc agttggatag agcaacggac ttctaatccg
    33661 tgtgccgggg gttcgactcc cttcaggcgc accatattat atggtgggta tagctcagtt
    33721 ggttagagcg ccagattgtg gctctggagg ccgtcggttc gaccccgatt acccacccca
    33781 aattattctt tggggtgtag ccaagcggta aggcacaaga ctttgactct tgcatctcgt
    33841 aggttcaaat cctgccaccc cagccaattt atttgagact cactagctca gtcggtagag
    33901 cacatgactt ttaatcatgg tgtccggagt tcgattctcc ggtgagtcac caaaacatgc
    33961 gggaatggcg gaattggcag acgcgctaga cttaggatct agtgtcaatg acgtatgggt
    34021 tcaagtccct tttcccgcac cattgtatgc gggtgtagtt caatggtaga acgtcagcct
    34081 tccaagctga tcgcgagggt tcgattccct tcacccgctc caaacatgcg cctgtagctc
    34141 agttggatag agcaacggac ttctaatccg tgtgccgggg gttcgactcc cttcaggcgc
    34201 accatgatgg taagataaac tagcttttta gttagttttt tttattttat aaatttatgg
    34261 agtcgaaccc gagagggcgc gagcgttaag caaaatgccc ctgtgcgggc attttgtagt
    34321 gagcgtagga gccctgcagg agagaaacga caaggggcga cgtcactagc atgcaagggt
    34381 ttacccgcag caggcgacga ctcccttcag gcgcaccata gcggaaagat aaactacctt
    34441 attataggta gttt
//
LOCUS       DAOOAB010000024        34404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1632_length_34404_cov_13.130222, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000024 DAOOAB010000000
VERSION     DAOOAB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 34404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34404
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_34404_cov_13.130222"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKU88_06905"
     CDS             complement(<1..377)
                     /locus_tag="PKU88_06905"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPX69042.1"
                     /translation="MAALKGNCLVAQSGGPTSVINASACGVIQEAFRSGEIDKVYGAL
                     HGVAGLLKEDLFLLNEESPEEIELMKVTPASALGSCRYKLKSIEASEGDYRRIIDVFQ
                     AHNIRYFFYIGGNDSMDTANKVS"
     gene            complement(436..1392)
                     /gene="pfkA"
                     /locus_tag="PKU88_06910"
     CDS             complement(436..1392)
                     /gene="pfkA"
                     /locus_tag="PKU88_06910"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPX69043.1"
                     /translation="MKNIAVLTSGGDAPGMNAAIRAVVRMGIISGFNVFGVRKGFRGL
                     INGDFEKMNESSVSDIIQRGGTILQTARSEEFKTEEGQDKAAKMAKIFDIDGLVVIGG
                     DGSFAGAKRLSEKGVACIGVPGTIDNDLAYTDYSIGFDTAVNNVLDAINRIRDTSSSH
                     ERASIIEVMGRYSGDIALYAGLAGGAEGIIVPEMGFELDDICRLLIERKNKGKLHSII
                     ILAEGAGDAKQLEQQIMENVDIEVRSTILGYIQRGGSTTAFDRILASRLGARAVELLA
                     EGKSSRVVGIRDNKLIDMEIVEALGMKKVFPRELYELAIKLQ"
     gene            complement(1477..1674)
                     /locus_tag="PKU88_06915"
     CDS             complement(1477..1674)
                     /locus_tag="PKU88_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69044.1"
                     /translation="MWTVVYLAKNKKIAEKISRLMTNEGVLVKLQPVNKNIGEEDSYV
                     EILVLESEVEEAHNILYELGY"
     gene            complement(1816..>3636)
                     /gene="dnaE"
                     /locus_tag="PKU88_06920"
     CDS             complement(1816..>3636)
                     /gene="dnaE"
                     /locus_tag="PKU88_06920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPX69045.1"
                     /translation="LTVIRDTLELIKAKGMEAPDMDSMDYDDPNVYKMISQGETYGIF
                     QLESGGMTQCFKELKPNCLEDIIAGISLYRPGAMDQIPKYIRNKHNPDKIRYMHPALE
                     HILDVTYGCIVYQEQVMQIVRDLGGYSMGRSDLVRRAMGKKKADVMAKERENFINGIV
                     DEAGNLVVKGCVRNGIPADTAADIFDEVAKFAGYGFNKSHAAAYAIIAYQTAWLKYYY
                     PVEFSAALITSVMGNSKKVAEYIQHCKSKGIEVLPPDVNESSVSFTVKENKIRFGLAA
                     VKNVGINAVLSIIKARQEKGRFDGLYDFLQKMDYSIINKRTVESLIKCGAFDSFGVYR
                     SRMLAVYEKLMESIQGQKKNNVEGQISLFDTIDDSENIIGNIYPDINEYPKKNMLSME
                     KEMLGLYISGHPLEEYKSELQASVTITTAELTPENEEEAQDIERNSELDGKRVIMGGI
                     IVSVKQKTTKTNNMMAFVELEDLYGTLEIIVFPKIYDRFKSLLVQDTIVLVEGRISQK
                     EEEAAKIICDAVRPLKKYAGRKLYIRINTGQQPDIIEQLKKVLIEYKGAQPVILVDEA
                     NKDNGKSRVMKADSSIWVDINDKLLNELKEVAGSDCVAVK"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3737..5149)
                     /gene="dnaE"
                     /locus_tag="PKU88_06925"
     CDS             complement(<3737..5149)
                     /gene="dnaE"
                     /locus_tag="PKU88_06925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPX69046.1"
                     /translation="MKNFVHLHVHTEYSLLDGAGRVEKLVARAKELGMESIAVTDHGV
                     MYGVMEFYKQAKKYGIKPLIGCEVYVAARTMCDRDPKLDGSQYHLVLLAKNEEGYNNL
                     IKLVSLGFTEGFYYKPRVDMEVLRKYSKGLIALSACLAGAIPGHIIDGNYEAAKRAAL
                     SYNEIFGQGNFYLELQDHGIKEQSLVNQEVVRMSRETGIPLIATNDVHYVDKEDAAAQ
                     DILLCIQTGKTILEEDRLKFQGQEFYLKSSEEMYELFKYVPDALENTAKIAEMCSLEF
                     NFGQVHLPAFDVPEGYTSDTYIRKLCYDGLAKRYAELTDELKERAEYELSVITRMGYS
                     DYYLIVWDYVKFARDNGIMVGPGRGSGAGSLVAYCLGITNIDPIKYNLIFERFLNPER
                     ISMPDFDVDFCYERRQEVIDYVVSKYGKDRVAQIITFGTMAARAAIRDVGRALNIPYA
                     QVDMIAKKIPFEIGMTIEKAL"
     gene            complement(5181..6524)
                     /locus_tag="PKU88_06930"
     CDS             complement(5181..6524)
                     /locus_tag="PKU88_06930"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPX69047.1"
                     /translation="MKKLEFDCSNSMIRDHELSCLREYVKSAHYMLHEKTGAGNDFLG
                     WVDYPKNYDRDEFERIIKAAGRIKDSSEVLLVIGIGGSYLGARTAVELLGHSFRNLLP
                     KDKRNAPEIYFLGNNISGTYFKELMEVIEGKSVSACVISKSGTTTEPAIAFRFVKSYL
                     EAKYGKQEAAKRIYAVTDASRGALKRLADAEGYESFVIADDIGGRYSVLTPVGLLPIA
                     AAGIDIRSILEGARDAWDEYNEPELDKNQCYRYAAVRSILAGKGKAIEVLINYEPSLQ
                     FFAEWWKQLFGESEGKDGKGIFPASMSFSTDLHSLGQFLQDGSRNMFETILNIEKPRF
                     EVEIINDEENLDGLNFISGKTMDFVNKKAFQGTMLAHMDGGVPINVLNIPEISEYYFG
                     EMVYFFEKACGISGYLLGVNPFDQPGVEEYKKNMFALLGKPGYEELKEKLDKKLR"
     gene            complement(6539..7495)
                     /locus_tag="PKU88_06935"
     CDS             complement(6539..7495)
                     /locus_tag="PKU88_06935"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HPX69048.1"
                     /translation="MLVGIDLGGTEIKAGLVDREGRILLQSSIPTNAGRDYRLIIKDM
                     QEQVEKLLKDYGAGIGDMESIGIGAPGLMNEKSGYVIYCTNLFWENVPLGIMLKEHFN
                     KPVYMGNDANAAALAESLFGSTKGVENSVLITIGTGVGSGIIIGHKIYSGSHFAGSEL
                     GHMIVGSNFYRCNCGNMGCLETFASATAMIRYAVNRLERDKIKSSILDKAGDTENIDA
                     RLIFDAAKEGDQLGIETVGRMIKYLSIGIINIFNILDPDIVAIGGGVSKAGAYLTDKL
                     KKEVGGMLFTSGIKYGDIVLAQLGNEAGLLGAAFLGINRNCV"
     gene            complement(7571..8638)
                     /locus_tag="PKU88_06940"
     CDS             complement(7571..8638)
                     /locus_tag="PKU88_06940"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPX69049.1"
                     /translation="MRKLPVDKVKEGMVLARTLYSNDGNILLNAGIILKNSYIAKFRD
                     LGIWEVYIDDEISRDIVLKDVISEETRFEARINIKNAMDSLRYGYSLEVKPIRNSVCK
                     MIDELMDEKDAMVNMQDLKSNDMYTFSHSVNVCVLSLITGISMKYDEEKLRELGMGAM
                     LHDIGKTRIPHEILNKPESLTKEEFELVKKHTSYGYELLKRSDVLSTYASYIALTHHE
                     RHDGEGYPLGLRGEEIHEFARIVSVADVFDAITSDRVYKKRINVNEAVEYLIGMGDHQ
                     FDYNIVRSFIEHITIYPPGTCVLLNSGEKAIVVDVNKKYPNRPIVRILADGEGQIPTV
                     PVEIDLTKNNALLISEIIDDI"
     gene            complement(8683..9639)
                     /gene="whiA"
                     /locus_tag="PKU88_06945"
     CDS             complement(8683..9639)
                     /gene="whiA"
                     /locus_tag="PKU88_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HPX69050.1"
                     /translation="MTFSSVTKNEISKIAVTNKCCQLALLSALIKMTGTLQIHGVERI
                     GITLSTENASIARMLFSLLKNCFDINTRVIVRKNRHLKKNNNYTLYIDSDIGSHEILK
                     MTGILQESDKGMKLNHKLPHYLIKKACCRKAYLRGIFLGSGSISDPEKTYHLELVTNN
                     ESFAEDIKDLMNHYQLGAKVVMRKGNFVVYIKEGENIVNFLNIIGAHNALLKLENIRI
                     YKEMRNNVNRIVNCETANLDKTLNAALRQIENIEYIKNTIGLNKLPEGLAEVAELRLE
                     FRDATLKELSEMMSPAIGKSGVNHRLRKLEQIAENLRDRHRE"
     gene            complement(9656..10180)
                     /gene="lepB"
                     /locus_tag="PKU88_06950"
     CDS             complement(9656..10180)
                     /gene="lepB"
                     /locus_tag="PKU88_06950"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPX69051.1"
                     /translation="MDENIKKEVISWVKLILSAFVIAFILKTYVFQIALVNQVSMEPT
                     LYEGEILVIAKVNYLINDPARGDIVVLKDELEDKYLIKRAIGLPNEVIDIRNDRVYID
                     GKELNPDYTDALTRDNGFAKSRVPEEKYFVMGDNRMQSRDSRSDTVGFVSRDNIVGKA
                     VFRIWPFNKFGIVR"
     gene            complement(10201..11217)
                     /locus_tag="PKU88_06955"
     CDS             complement(10201..11217)
                     /locus_tag="PKU88_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HPX69052.1"
                     /translation="MKNNSVKNTLHGKGIIPGGPKIVAIGGGTGLSVLLRGLKKHTSN
                     ITAIVTVSDDGGGSGVLREDMGILPPGDIRNCIVALANIEPIMEQLMQYRFREGKLKG
                     QSFGNLFIAAMNDICGSFDAAVREVSSVLAVTGKVLPVTLEDVVLYAELEDGTIIKGE
                     SQIPIKQRALNKRIRKVFLEPGNCMPMPEALREIQEADAVVFGPGSLYTSIIPNILVK
                     SIAEAINSTKAMKIYVCNIMTQQGETIGYSLSEHIKAINYHGNRAPIDYAIANTGEIP
                     QALLEKYKTEDASPVLIDYDIIADMGIKVIEGDFVNIENGYLRHDYNKLAEQIFKLLN
                     EEQK"
     gene            complement(11244..12110)
                     /gene="rapZ"
                     /locus_tag="PKU88_06960"
     CDS             complement(11244..12110)
                     /gene="rapZ"
                     /locus_tag="PKU88_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPX69053.1"
                     /translation="MKFLIVTGLSGAGKSQSIKFLEDLGYFCVDNLPPALLGRFVEMC
                     SESKGHIDKAAIVIDIRVGKFLDQLPVGLDELKELGHTYDILFLEASDETLIKRFKSS
                     RRMHPLSVGGRIIKGIKAERRKLQEIKSKATNIIDTTNMSPSELKQEITHLFVEGGKS
                     EGLIIHVISFGYKYGIPLDSDMVFDVRFLPNPYYVEDLKKFSGADAGVRNYVMQWPAA
                     AGFMDKLNDMLEFLIPHFIKEGKSQLVISIGCTGGRHRSVTMANMLSNKLKEANHRVI
                     VEHRDIEEERYR"
     gene            complement(12127..12858)
                     /locus_tag="PKU88_06965"
     CDS             complement(12127..12858)
                     /locus_tag="PKU88_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPX69054.1"
                     /translation="MKLFADYHTHTVYSHGTGSILDNVTAAREKGLSEIAVTDHGIRH
                     FAYGVRIKDIAKMRDEIDRINYNTEGIKVLMGMECNIISTAGDIDMDDSIRKYLDILL
                     VGFHMMVLPKTAGDAVNIYGRNYLSKIFHLGTEGIKEANTESMIRAIRKHKIDIITHP
                     GTRIPLDISLVAREAAKAGTALEINSHSSYMKAKHVIAAAEEGAEFVINSDAHAPEDV
                     GNLQNGLNIAMEAGLTCDQIINAVK"
     gene            complement(13434..14300)
                     /gene="murB"
                     /locus_tag="PKU88_06970"
     CDS             complement(13434..14300)
                     /gene="murB"
                     /locus_tag="PKU88_06970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPX69055.1"
                     /translation="MQILYNEPMKNHTSFKVGGPADIFIEPDDMAELVKAIKDLKEQC
                     VSYYIMGNGSNLLVSDKGLRGAVVKIGEKFGSVSINNDIITAESGVLLSTLSKMAAKE
                     GLTGMEFASGIPGSLGGAVTMNAGAYGGEMKDIVEWVEVLDQDLNLKRLKNDEMKFMY
                     RKSIIEPGKHIVLRCCIRLKKGNKDEIDNKMAELAEKRKSKQPLHLPSAGSTFKRPEG
                     YFAGKLIEDAGLRGFSIGGAQISTLHCGFVVNNGDATAKDVYDLIRHVQKTVFEKFNV
                     MLEPEVKILGKF"
     gene            complement(14325..15032)
                     /locus_tag="PKU88_06975"
     CDS             complement(14325..15032)
                     /locus_tag="PKU88_06975"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPX69056.1"
                     /translation="MTEVKSTNKEKQPVIEYNGKKYLRIPVKTHVITKDDSIVDVVSM
                     YAREIVKENDIVFISEKAVAITQGRAYPLEEIKPRPLARLLSKYVTKTPSGIGLGIPE
                     TMEMALRECGVLRILFAAVVSFFGKLFGRKGDFYRIAGYKASSIDGPTPNTLPPYNRY
                     VVLGPENPDQVAKSISDSIKAEVAIVDINDLGGSILGTSKSSLDRKALAGILRDNPLG
                     QCCEQTPMGIIRSAEEQ"
     gene            complement(15111..16460)
                     /locus_tag="PKU88_06980"
     CDS             complement(15111..16460)
                     /locus_tag="PKU88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPX69057.1"
                     /translation="MTLRKAFTLFLCGILTLYIFSGPVLLEHVKEDYKKSLEPVHEKT
                     EWTGVITLWDYPRLDIRSGTRFSWIKSKIGQFEKQNPGVYIELRELDWNEGPGFLKAA
                     AKIGANPDIAPIGSDYFFISGGYLEELDGYISLEERADFIDSTLDTAMYNGKTYGIPW
                     MMTGYTMLLNTDIFSERNVSLPENGEWTYEQFVEALKQLTYNGIYGFNSFIGPGYYNT
                     FGIIMCDGAQIIDDTTGRYIFNTPEAVSGLTKLCDLKHKYKVSHPDFGAMNENQAWSA
                     FLNGKTAVYIGGSWTVPLLRNIQGNNGINFAVAKFPTGKSDVPLSLSSTVCSYAVFKQ
                     KDAAKKKVCIDFIKFITSKESQKELGYLGYFPVRKSGKYLYENDKEMYTIQQNLYFAE
                     HLPKADNWSEIDLILQTRIKEAVQGNISPEQAVKEAEKLIEQRRKQGVAAEAGSAAP"
     gene            complement(16729..17454)
                     /locus_tag="PKU88_06985"
     CDS             complement(16729..17454)
                     /locus_tag="PKU88_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPX69058.1"
                     /translation="MKLLLDTHCHTISSGHAYSTIKEIAEEAADMGLKLIAMTDHGPD
                     MQGGPDLFHFGNMRVIPEYIKGVRVLKGVEANIMDFEGRLDVPEKYLKKLDIVIASLH
                     EACIDSGTVEENTRALIGAMSNPFVDIIAHPGNPYYPVDIDRLLQCAKDTGTLIEINN
                     SSFLGSRKGSSENCRIIAAKAKEMGVVLAAGSDCHICYDVGKFNKIEEIFEELEMPEA
                     LVINTDPLKLIDYLNKKGKKVSL"
     gene            complement(17471..17704)
                     /locus_tag="PKU88_06990"
     CDS             complement(17471..17704)
                     /locus_tag="PKU88_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPX69059.1"
                     /translation="MYKKLYKSRTVRTISGVCGGLEDYFNIDVTIIRLIWVALTIATG
                     FFPGIFAYVAAALIIPDEPTGYNPEYKVEKDEQ"
     gene            complement(17831..19693)
                     /gene="uvrC"
                     /locus_tag="PKU88_06995"
     CDS             complement(17831..19693)
                     /gene="uvrC"
                     /locus_tag="PKU88_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPX69060.1"
                     /translation="MISLEDKLKNLPEKPGVYIMRDEYNEIIYIGKAINLKNRVRQYF
                     QNSKNQNPKVAAMVERITDLEYIITDSELEALILECNLIKKHRPKYNVMLKDDKQYPY
                     IKITVKEDYPRLIIVREIKKDGAKYFGPYTDVTAVNRTIDLLKGLFPIRYCNKNISRA
                     IGKERPCLNYHIKKCIGPCRGNVSKEQYRELIDSIIMMLEGKQEEVLKGLEDKMHQAA
                     DNLDFEKAAEIRDNMNSLIKIAEKQKVISSALIDQDIIAFALNESESCIQVFFVRGGK
                     LIGREHFLIEDTKLSDSKEILSSFIKQYYGSDALIPREIILQEEIDEINIIERWLSDK
                     KGGRVKLVVPQRGEKQKLIEMVGRNAEDTLRLLIEKQKNDTEKTMGACRELKDILELN
                     AVPERIEAFDISNIQGVLNVGSMIVFEQGKPNNRNYRRFKIKSVEGANDYESMREIIE
                     RRFAHGLKEREELEAEEKDFELGRFSVFPDVIMLDGGVGQVNAVLPVLRELNINIPVC
                     GMVKDDKHRTRGLIYSGREIELPKGSKVFRLISAIQEEAHRFAISYHKSLRSKTSVKS
                     ALDDIPGIGPARRKALLKSFGSLKNIKKASLEELEEVQGMNRKVAEVVYNFFNS"
     gene            complement(20022..>21682)
                     /locus_tag="PKU88_07000"
     CDS             complement(20022..>21682)
                     /locus_tag="PKU88_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPX69061.1"
                     /translation="FVLGYGENGDYEKWESKGRINKSKANELIEKYKDDSYVERAFEE
                     LREYWDELLSGYQLLSSEEKLNRMVNIWNPYQCMITFNLSRSASYFETGIGRGIGFRD
                     SNQDILGFVHMIPDKARSRILELAGTMLEDGGAYHQYQPLTGKGNNEIGGEFNDDPLW
                     MILSTITYIKETGDYSILDEKMPYNCDANKLGTLKEHLEKAFEYTMKNLGPHGLPLIG
                     RADWNDCLNLNCFSINPDESFQTCSNRNGKTAESVFIAGLFVYVGREFGRLCRLLGYD
                     AQASGIEEQVDKMRGSIMKYGCDENWFLRAYDYFGNRVGSAENEEGQLFIEPQGICVM
                     ADIGLENGMALKALDSVHERLNTGYGLVLQDPAYSEYKLNLGEISSYPPGLKENAGVF
                     CHNNPWIMIAETRMGRGSRAFEYYSKIAPAYIEDISDIHKMEPYVYTQMIAGKDAKNP
                     GEAKNSWLTGTAAWNYEAVTKWILGIRPDFEGLIIDPCIPEEWDGFKVIRKFRNTAYN
                     ISVKNPEHVSKGIKRVRVDGKTAASNLIPVFNDELTHDIEVIMG"
     assembly_gap    21683..21782
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21783..22612)
                     /locus_tag="PKU88_07005"
     CDS             complement(<21783..22612)
                     /locus_tag="PKU88_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPX69062.1"
                     /translation="MRFGYFDDLNREYVINTPKTPFPWINYLGQDGFFSLISNTAGGY
                     CFYKDARLRRLTRYRYNSIPWDNGGRYLYVNDSGKVYSLTWMPVRNDAESFECRHGLG
                     YTKIKCLQNRLESEVLYFVPLKDNAELHHLRLVNRGEEDKEITLFSFIEFCLWNAYDD
                     MTNYQRTFSTGAVEVEGSTIFHKTDYRERRNHYSFYSVNRKIDGFDTDRDSFLGRYNG
                     LHEPEAVFEGKSRNSIAEGWNPIASHEIRIKLKPGEQTTLLFVLGYGENGDYEKWESK
                     G"
     gene            complement(22609..>24860)
                     /locus_tag="PKU88_07010"
     CDS             complement(22609..>24860)
                     /locus_tag="PKU88_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPX69063.1"
                     /translation="SEFKGETLYVRIRGKNAYTLFSPYFVPTLDQYQMYECHVGLGYT
                     RIVSEFYDIRTEALVFVPLGGNREIRDIRITNLRAVPVELDVIPVIEYSHFEALKQLI
                     NADWVPQTMRSECIEEHRGYRTLIQYAYMNKDTRINFFTSNHAASSYETDRRYFLGDN
                     EYGTWESPGSLNNSELGNHEVLRGDNIAAMMHHIGILEPGESRRIITQLGQCRSITEE
                     KESMESFRSEERVNEEFEKLKYFWEEYLSKLQVETPDKAMNTMLNIHNPRQCYITKNW
                     SRYLSLYQLGYGSRGIGFRDSAQDVMGIMDSAPEEGRELIEKLLRVQKRNGSAMHQFN
                     PATMIANEGDSRDFEDRPKYYSDDHLWIVLAVCAYLKETGNMDFLNKTVPFYEKDKSG
                     MPIEKGTVLEHLERAVGFTGNDLGRHGLPLLGFADWNDTVNLPAGAESVFTACLYGKC
                     LSEMIELMDFLGKKKLKEMYRGRYQRMRERVNSCGWDGEWYISYFDYEGNPIGSKVNS
                     SGQIYANAQSWAVISGFAGEDRALKSLDSVRSILNTRNGIKLSYPGYKGYDPEKGGIT
                     TYPPGTKENGGIFLHSNPWVMIAETMVGNGDRAFEYYSQINPACKNGCIDEYQCEPYC
                     YPQNMLGDEHPQFGLARNSWLSGTASWVYQAAVKYILGVRPHYKGMTIDPCIPRAWKE
                     FRVRKHFRGSIYDIRVRNPEGVSKGIRAIWVDGTVFFRNVLPCFRDNRLHNIEVLMGR
                     DLFLTEEDR"
     assembly_gap    24861..24960
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24961..25205)
                     /locus_tag="PKU88_07015"
     CDS             complement(<24961..25205)
                     /locus_tag="PKU88_07015"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69064.1"
                     /translation="MQYGYFNDINREYVITDPRTPVKWINYIGSLDFGGFIDHTGGAL
                     ICKGDPAVNRIIKYIPQLPASEFKGETLYVRIRGKNA"
     gene            complement(25240..25848)
                     /locus_tag="PKU88_07020"
     CDS             complement(25240..25848)
                     /locus_tag="PKU88_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HPX69065.1"
                     /translation="MKWLFNTEGIIYKIMTAVYQMIILNLLWCITSIPLITVGASTTA
                     LYYVIGKIARKEEVHEIKDFYKSFRENFKQATCIWMILCAAYVMIYTNLSFLEKYGSM
                     GGLMLAVQLPVLVQVVIITVFVFPLLSRYESSTIGIIKASWVLGIKHVFSCFGTLAIV
                     AAAALIARTVPGLFLLVFISFTAFAIYLVVGRVMEKYRPGAV"
     gene            complement(25941..>26849)
                     /locus_tag="PKU88_07025"
     CDS             complement(25941..>26849)
                     /locus_tag="PKU88_07025"
                     /inference="COORDINATES: protein motif:HMM:NF023435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX69066.1"
                     /translation="KVIKEKEPNVYGLEALNGESPYKLMDWDVIGDDKCPGVVYNDSK
                     DLVVFNEFEKPETMALLKTIRKFFQAGYIRKDAATVTDYNADEAAGKIFAATKSLKPG
                     KDAEMSLTHGFEWVQVYITPPVISSRDTMGSLQAISSTSKNPERALMFLELFNTDPYL
                     NNLINFGIEGKHYVKVSDSVIKAGPDQTKYNPGTGWMFGNQFINYLYESEDPKKWEQF
                     EEFNQQAAGTQTLGFNFDPSPVKNEIAAVNNLWDKYVPALETGSIDPEVNVPKFLEEL
                     KAAGIDKVIAEKQEQLNEWAEKMGKK"
     assembly_gap    26850..26949
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26950..27311)
                     /locus_tag="PKU88_07030"
     CDS             complement(<26950..27311)
                     /locus_tag="PKU88_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX69067.1"
                     /translation="MFKTGKLLCLTLVLVFALASLTGCAKPAAAPAPEPAEAGSGTTL
                     EPVELTWFFIGNGQQEDVSLIEEKINEYIKDKINATVKLQCYQWGAEYDDKLRQMIAS
                     RQEFDICFTAAWANNYMQ"
     gene            complement(27372..28268)
                     /locus_tag="PKU88_07035"
     CDS             complement(27372..28268)
                     /locus_tag="PKU88_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPX69068.1"
                     /translation="MRDFIKINRKADIALNIVMILCTIACVLPLLIVFSASITDEKTV
                     TMYGYSLFPRNLSLEAYKYIYKDIMVILRAYGVTVFVTVVGSALSLVTIALYAYPISR
                     KDFKYRNVFTFIVFLTMLFNGGLVPWYMVYVNVLHIDDTIMALILPYLMTPLYVLIMR
                     TFFMTTIPDGIIEAARIDGAGEFQTFVWIVLPLAKPVLATIGLFNILTYWNDWYAPLL
                     FINNEKLFNLQYLMYRVDKAIIYLSNNAGSINNATQIMANLPSQTARMAMAVVAIGPI
                     ILAYPFFQKYFVEGLTIGSIKG"
     gene            complement(28282..29241)
                     /locus_tag="PKU88_07040"
     CDS             complement(28282..29241)
                     /locus_tag="PKU88_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPX69069.1"
                     /translation="MQRASGTEITTVRRQKPALLKELAKNKVLYLMLLPGLLVLLVNN
                     YLPMFGIVIAFKDYNYAKGFWKSDWVGFNNFEYLFNSKAAFLITRNTILYNVAFIVIA
                     ATVAVVLAIIFNELLNRRISKFYQSVMFLPYFFSWVVISYLVFALLSSDMGFVNKILI
                     SNGMQPVEWYVDPRFWPLIIILVNTWKWTGYDSIIYLAAIIGFDKSLYEAAAVDGASR
                     FQQITKVMVPMLMPVITVLTLIKVGRIFFTDFGLFFNIPRNSGPLYDVTNVIETYVFR
                     ALRQTGDIGMASAAGFYQAVVGFIVVMTANYIVRKIDKDSSLF"
     assembly_gap    29434..29533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29534..31125)
                     /locus_tag="PKU88_07045"
     CDS             complement(<29534..31125)
                     /locus_tag="PKU88_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX69070.1"
                     /translation="MNRFRKETRAMYKVLIVDDESIIRKGLKNIIDWKSLDCEVCGEA
                     GDGMEGIRMIRELRPHILITDINMPEIDGLSMIKETKQLLPGSKIIILTGYRDFEYIQ
                     EAIRLGAFDYLLKPSKIEDLTAIVQKAIAELQRSCKMEEELNNLKKHFKSKIPVLRQK
                     LLYDILFRINTKKYEILEELNLYGLTIDKFKIMAVETDEEGQENAKSQYEKHLYQFGI
                     INTFEEILGTSFKTADVPINSRTILFVIQPNEESEGDMFSSLYKDAVSLQELVRNCFS
                     FTVTISISSEGNGALELADKAREALEALDYKFYMGKNTIILYEDLKSFYKSADYSALE
                     VYEKSLVQMVKSGNKDSVSLVMKDILKCVNECKLDRESIKKFYWNLINLVCSIPTTMK
                     LNDESFHYRRITEIFAALDQCESIIEINGILEEAALNIAERINSFNRKTINSTLQKAM
                     DYICLNYNEPITLNEVAEHTYVSIYYLSRMFTKELGKNYVDYLNEVRIEKAKEYLKDS
                     NCKTYEIAELVGIKDAHYFSKL"
     gene            complement(31088..32902)
                     /locus_tag="PKU88_07050"
     CDS             complement(31088..32902)
                     /locus_tag="PKU88_07050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPX69071.1"
                     /translation="MESIINKFVLFYKNTSIKNKLLLLFYIQIVIPLFFIGITSYQKS
                     AEIIEQKSINYSLDILKMIELRFKDLSTGMQGLTLELLYDNRVYDALSSKGYEDNITI
                     YNRANEIRSILRQATLSRNEIQSICLVTKNRRFYSFDSDNGKVRIEFILPYDSIVEAA
                     RKGRGRPVWFLEKQEGRESEIYVTRMVYDRDSYNEIGLIAINIKKEYLESVYTDLSRE
                     SLNNISILSENNEEIIKQQENSGILKNFYRQQLQGRRGFYKDNKAGMLVSYVLLEDPS
                     WKIVYHIPLKELYREMDTLKRWVLLIIVYGLIILSVLSVLTSVDIITPINKLVEGMKK
                     VEKGNKHEDIELDRSDELGYLSESFNRMSKKIDYLVNRIYKEEIALKEAEIKALQAQI
                     NPHFLFNTLENINWMARLNGVPEISETVSALAKLIDGSIGRGDRTISLKEELEYIDNY
                     MTVLKNRYEDRLEVIKILDEGLMDKKIPRLLIQPLVENAVKHGIGKSRRKGVIRLEAF
                     REEGHIVFEVEDNGMGMTAEELEALNKRLQEDELILEGNGTAPARKSIGLENVNRRIK
                     LLYGSSYGVKIESSYDEYTKVTVRIPDEQVQEGDKGNV"
     gene            complement(32915..34234)
                     /locus_tag="PKU88_07055"
     CDS             complement(32915..34234)
                     /locus_tag="PKU88_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPX69072.1"
                     /translation="MKIKKSDIYYLAIFLALAIIPVMYTIKLYDRGIPADSFEGEKET
                     IELRFLSSWGGTDTKAQQLQMLLDEFEEQNPGVKVINESMSGSEFLFKLKTNFAQGSD
                     PDVFGLWPGSDIKILINQGKVADLTELLASDPEWAAGFGEDAWSYDRFNGRIYGLPCE
                     IIYEGLFVNRDLFEKYGIKVPETYEELKDAVRAFRQRDIIPIAYNSTPEGTFLYQNIV
                     MKLGGREYTENPYKEGRINENYIEGMKYVKELYELGAFPEDAFVIADKTRDNLFLEKE
                     AAMIVQGSWFIGLGSLGAEDETVDIIPFPAFKEGKSHTSSIIYGLGNGNFHMSRKAYE
                     NPDKREMCIKLLKHLTSVEAAKLFSHETGFISNIKIPDQSVQPGRLMEKGKELIARSM
                     ELVGPTDSFIDRNLWEQVLVTSFPHVLEGRMTPEEVFEEMERRAAQQ"
BASE COUNT         8203 a         8777 c         6077 g        10847 t
ORIGIN      
        1 tggcttacct tgtttgccgt atccatcgaa tcgtttccgc ctatgtaaaa gaagtacctt
       61 atattgtgtg cctgaaatac atctataatc cttctgtaat ccccttcact ggcttcaatg
      121 gacttcagct tgtacctgca ggagcccagg gcggatgcgg gtgttacctt catcagctca
      181 atttcctcgg gtgattcctc attcagcagg aacaggtctt ccttaagcaa ccccgccaca
      241 ccgtgaagcg ccccgtaaac cttatcaatt tcaccggacc tgaatgcctc ctgtataaca
      301 ccgcaagcgc tggcattaat tacagaagtc gggcctccgg attgtgccac caggcaattt
      361 ccctttaatg cagccataat tcatctctcc ttttcaagtt ttgttatgtt atataataag
      421 tctcgagcct attatctact gtagtttgat tgccaactca taaagctccc gcgggaatac
      481 ttttttcata ccaagggctt ctactatttc catatctatc agcttattat cccttatacc
      541 cactaccctt gacgattttc cttccgccag cagctccact gccctggcac caagcctgct
      601 tgccagtatc ctgtcaaagg cggttgtgct tccgcctctt tgtatatacc ccaatatggt
      661 tgaccttacc tcaatatcca cattctccat tatctgctgc tccagctgct ttgcgtcacc
      721 tgctccttcc gcaaggatta ttatactgtg aagcttgcct ttgtttttcc tctctatcag
      781 cagcctgcag atatcatcca gctcaaatcc catctcaggt actatgatgc cttccgctcc
      841 tcccgccagt cccgcataca gcgctatatc cccggagtac cttcccatta cctcaataat
      901 acttgccctt tcatgggagg atgaagtatc tcttattctg ttaattgcat ccagcacatt
      961 atttacagct gtatcaaagc ctattgaata atcagtataa gccaggtcat tgtcaatggt
     1021 gccgggcacc cctatgcagg caacgccttt ttccgaaagc cttttggctc cggcaaaaga
     1081 cccgtcccct ccaataacca ccaaaccgtc aatatcaaaa atctttgcca ttttggctgc
     1141 cttatcctgg ccttcttctg tcttgaattc ttcacttctg gccgtctgca gtatagttcc
     1201 gcctctttgt ataatatcag atacggagga ttcgttcatt ttttcaaaat ccccgttaat
     1261 aagcccccgg aagcccttcc ttaccccaaa tacattaaac ccgctgatta tgcccattct
     1321 tacaactgcc cgtatggcgg cgttcatccc cggcgcatcg cctccgctgg ttaatactgc
     1381 aatattcttc atgggtgtac ctccttttgt cctagatggt cataatatgt cccaatattg
     1441 tatgaaaata tatttaatat agtgtctgat accatattaa tatccaagtt catataaaat
     1501 attgtgtgct tcctccacct cagattcaag taccaatatt tcaacataac tgtcttcttc
     1561 accgatattt ttgtttaccg gctgcagttt aacgagaacc ccctcgtttg tcatcagcct
     1621 gcttatcttt tcggcgattt tcttgttctt tgccagatat acaactgtcc acataattgt
     1681 tgcctccttt attgtcaaac ctttatgtcc gcaaaatatt acatatactt caaatctctg
     1741 ttggtggagc aacttcttgc ttagaaataa ttttaaatga aatgcccttt aatatcaaga
     1801 ttattctata tatcgttact tgactgccac acaatcggat cctgctacct ctttcagctc
     1861 attaagcagc ttatcattta tatcaaccca aatactgctg tcagctttca ttaccctgct
     1921 ctttccgtta tccttattag cttcgtctac caaaattacc ggctgtgccc ctttatattc
     1981 aatcagcact tttttcaact gctcaataat atccggctgc tgcccggtgt ttattcttat
     2041 gtaaagcttc ctgcctgcat actttttcaa aggcctgaca gcatcacata ttatctttgc
     2101 agcctcttct tccttctggc ttattctgcc ctccaccaga actattgtat cctgaacaag
     2161 aaggctcttg aatctgtcat atattttggg gaacactatt atttccaaag taccgtacaa
     2221 atcctccaat tccacaaaag ccatcatatt gtttgttttt gtggtttttt gctttacaga
     2281 tactatgatg ccccccatga tcaccctttt cccgtccaac tcggaattcc tttcaatatc
     2341 ctgtgcttcc tcctcatttt cgggggtcag ctctgctgtt gttattgtca ctgatgcctg
     2401 gagttccgac ttatactctt caaggggatg cccgcttata tacagcccca gcatctcttt
     2461 ttccatggaa agcatgtttt tcttaggata ttcatttata tcgggatata tgtttcctat
     2521 tatattctca ctgtcatcta tggtatcaaa cagtgaaatc tggccctcca cattgttttt
     2581 cttctggcct tgtatgcttt ccataagctt ttcgtacacc gccagcatac ggctcctgta
     2641 tactccaaag ctgtcaaagg cgccgcattt tatgaggctt tccaccgttc tcttgtttat
     2701 aatgctgtaa tccatcttct gaagaaagtc atacaacccg tcaaaccttc ccttttcctg
     2761 tcttgctttg ataatggaca gaactgcatt tattcctacg ttctttactg ctgccaatcc
     2821 gaaccttatc ttgttctcct ttaccgtgaa gcttacgctg ctttcattca catccggcgg
     2881 aagcacctca atgcccttgc ttttgcagtg ctgtatgtat tccgctacct tcttgctgtt
     2941 tcccataaca ctggttataa gtgccgctga aaactccaca gggtaataat actttagcca
     3001 tgctgtctga taagctatta tggcatatgc agccgcatgg gacttattaa agccgtagcc
     3061 tgcaaacttg gctacctcat cgaaaatatc cgctgcagta tctgcaggta tcccatttct
     3121 gacacagccc ttaaccacta aatttccggc ttcgtcaact atgccgttta tgaagttttc
     3181 cctctccttt gccattacgt cggctttctt ttttcccatg gcccttctca caagatcgga
     3241 cctgcccatg gaatagcctc ccaggtctct tactatctgc attacctgtt cctggtacac
     3301 tatgcatcca taggttacat ccagaatatg ttcgagggcg ggatgcatat atctaatctt
     3361 atccggattg tgcttgtttc tgatatattt aggaatctga tccattgctc ccggcctgta
     3421 cagggatatc cccgctataa tatcctccag gcagttgggc ttcagctcct tgaagcactg
     3481 ggtcattcct ccgctttcca attggaatat tccgtaagtc tctccctggg atatcatttt
     3541 gtacacattg gggtcatcat aatccatgct gtccatatcg ggggcttcca ttcccttcgc
     3601 ctttataagc tccagggtat cccttatcac tgtcagnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnncagt gccttttcta tagtcattcc tatctcaaag gggatttttt
     3781 ttgcaatcat atctacctgg gcatacggaa tgttcagtgc tctgcccaca tcccttatag
     3841 cggctcttgc agccatggtg ccgaaggtga ttatttgagc caccctgtcc ttaccgtatt
     3901 ttgacacaac atagtcaata acctcctgac gcctttcata gcagaagtcc acatcaaaat
     3961 ccggcatgct tatcctctca gggtttaaga atctttcaaa tatcagattg tatttgatag
     4021 gatcgatatt ggttattccg aggcagtagg caacaaggct tccggctccc gaaccccttc
     4081 cgggccctac cattatcccg ttgtcccttg caaactttac ataatcccaa acaataagat
     4141 agtaatcgga atatcccatt ctggttatta ccgacagctc atattccgcc cgctctttga
     4201 gttcatccgt caattcggca tatcttttcg ccaaaccgtc ataacagagc tttctgatat
     4261 aagtatccga ggtatagcct tcgggaacat caaaagccgg aagatgcacc tgtccgaaat
     4321 tgaattccag gctgcacatt tccgctattt tcgctgtgtt ctccagggca tcgggaacgt
     4381 atttgaaaag ctcgtacatt tcctcggaag attttaaata aaactcctgg ccctgaaatt
     4441 tgagcctgtc ttcctcaaga atagttttgc ctgtttgaat acaaagcaaa atatcctgtg
     4501 ccgcggcatc ctccttgtcc acatagtgca catcgtttgt agcaatcaaa ggtatccccg
     4561 tctcccggga cattcttaca acctcttgat taaccagtga ctgctccttt atgccgtgat
     4621 cctgaagctc cagataaaaa ttcccctggc caaaaatctc attgtatgac aatgccgctc
     4681 tttttgcggc ctcgtagtta ccgtcaatta tatggcctgg tatcgctcct gcgagacagg
     4741 cgctcaatgc aatcaaccct ttggaatact tgcggagtac ttccatatct actctcggct
     4801 tatagtaaaa accctccgtg aagcccaagg aaactaactt tatcagatta ttgtagcctt
     4861 cttcattctt tgccaaaagc acaaggtgat actggctgcc atccagcttg ggatccctgt
     4921 cgcacattgt tcttgcggcg acatatacct cacagccgat taacggtttg attccatatt
     4981 tttttgcctg cttgtaaaac tccatcacac cgtacataac gccgtgatca gttaccgcta
     5041 ttgattccat gcccagttcc ttcgctctgg ccacaagctt ttcaactctg cccgctccgt
     5101 ctaaaaggct gtattccgta tgcacatgga gatgcacaaa gttcttcata agtatttctc
     5161 ccctgtggta cgagtaccac ctatcttaat tttttgtcca gcttttcctt taattcttca
     5221 taacccggtt tgcccagcag cgcaaacata ttctttttat attcctcaac cccgggctga
     5281 tcaaagggat tgacgccaag cagatacccg cttataccgc atgccttttc aaagaaataa
     5341 accatttccc cgaaataata ctcgctgatt tcgggaatgt tgagtacatt tatcggtact
     5401 cctccgtcca tatgggctag cattgtaccc tgaaacgcct ttttattcac aaaatccatc
     5461 gtctttccgc tgataaaatt caaaccgtcc aggttttctt catcatttat tatctctact
     5521 tcaaatctgg gcttttctat attaaggatg gtctcaaaca tatttctgct gccgtcctga
     5581 aggaattgcc ccagggagtg cagatcggta gaaaagctca ttgatgcggg aaatatccct
     5641 ttcccgtcct ttccttcgct ttcgccgaac aactgcttcc accactcggc aaagaattgc
     5701 agggaaggct catagtttat caggacttca atagccttgc cctttccggc aagtatgctt
     5761 ctcaccgccg cataacggta acactggttt ttatccagtt ccggctcatt gtattcatcc
     5821 caggcatccc ttgcaccttc aaggatgctt cttatatcaa ttcctgccgc tgctattgga
     5881 agaagcccta cgggagtaag caccgagtac ctgcccccta tatcatcggc tataacaaag
     5941 gattcatagc cttctgcatc tgcaagcctt ttaagtgcgc ccctggaggc atcggtaacg
     6001 gcatatatcc ttttcgccgc ttcctgcttt ccgtatttcg cctccagata tgacttaaca
     6061 aacctgaagg caatagcggg ctcggtagta gtccctgact tcgatatgac acaggcgctt
     6121 acgctttttc cctctatcac ttccataagc tccttgaagt atgtgccgct tatattgttt
     6181 ccgaggaaat atatctcagg agcgtttctc ttatcctttg gcagcaggtt gcggaaggaa
     6241 tgccccaaaa gctcaacggc agtccttgct ccaaggtatg aaccccctat gcctattacc
     6301 agcaatactt ccgaggaatc ctttatccgt ccggcagctt ttataatcct ttcaaattca
     6361 tccctgtcat agttttttgg atagtccacc catccgagaa aatcattgcc cgctcctgtt
     6421 ttttcgtgga gcatgtaatg ggcagacttc acatattctc ttaaacaaga taattcatga
     6481 tcccttatca tggaattgga acaatcaaat tcaagctttt tcataacttc ctccttcatt
     6541 acacgcagtt cctgttaatc cccaggaaag ccgctcccag cagccccgct tcatttccaa
     6601 gctgcgccaa tactatatcc ccgtatttga ttccggatgt aaacagcatc ccgccgactt
     6661 cctttttcag cttatccgta agataggctc cggctttgga taccccaccg cctattgcta
     6721 ctatatccgg gtccagaata ttgaatatat ttattattcc tattgaaaga tactttatca
     6781 tcctgcctac agtctcaata ccaagctgat ccccttcctt cgccgcatca aaaatcaacc
     6841 tggcatcgat gttttctgta tcccccgcct tatccaaaat gcttgactta attttatccc
     6901 tttcaagcct gttgactgca taccttatca ttgcagttgc cgaggcaaaa gtctccaggc
     6961 agcccatatt gccgcagttg catctgtaaa aattggaccc tacaatcata tgcccgagtt
     7021 cggaacccgc aaagtggctt ccgctgtata tcttatggcc tattattatt ccgctgccta
     7081 cacccgttcc tattgttatc agcacggaat tttcaacacc ctttgtggag ccgaaaaggc
     7141 tttcggccaa agcggcggca ttggcatcgt ttcccatata tacaggcttg ttgaaatgct
     7201 ccttaagcat aatgcccaaa ggaacattct cccagaaaag attagtacag tatatgacat
     7261 atccgctttt ctcattcatc agcccggggg caccgatgcc tatggattcc atatcgccta
     7321 tgcctgcccc atagtccttg agcagctttt ccacctgctc ctgcatatct ttaattatca
     7381 gcctgtaatc tctgcctgca ttagtaggaa tggaagactg cagaagtatc cttccctccc
     7441 tgtccaccaa acctgctttt atctcagtac ctcccaggtc aattccgaca agcattgccg
     7501 cacctccttg tacagacgtc atatatggtt actgttacgg acgccatata tggcgtccgt
     7561 acccggattg ttatatatca tctatgattt cacttattaa aagtgcatta ttctttgtca
     7621 gatcaatctc aacaggcact gtgggtatct gcccctctcc atctgcaagt attcttacaa
     7681 tcggcctgtt gggatatttc ttgttcacat ccactactat ggccttttcc ccgctattta
     7741 aaagtacgca ggttccaggc ggatatatgg ttatgtgctc gataaaactc cttacgatat
     7801 tgtagtcgaa ttggtgatct cccattccaa tcaaatattc aacagcttca tttacattga
     7861 tcctcttctt gtataccctg tccgaagtta tggcatcaaa cacatctgca acggatacta
     7921 ttctggcaaa ctcgtgtatt tcctcccccc tcagccccag aggatagccc tcaccatcat
     7981 gcctctcatg gtgggtcaat gctatatagc ttgcataggt gctcagcaca tcgcttcttt
     8041 taagcagttc atagccatag cttgtatgct ttttgaccag ttcaaactcc tctttcgtaa
     8101 ggctttcggg tttattcaat atctcatggg gtatccttgt cttgccgatg tcatggagca
     8161 ttgcgcccat tccaagctct ctgagctttt cttcatcata tttcattgat atgcctgtaa
     8221 tcagagacaa tacgcatacg ttaacgctgt ggctgaaggt atacatgtca ttgctcttca
     8281 aatcctgcat atttaccata gcatcctttt catccataag ctcgtcaatc attttgcata
     8341 cgctattcct gatgggtttg acttcaaggg agtatccgta tcttaagctg tccattgcgt
     8401 tctttatgtt tattctggct tcgaatctcg tttcttccga tataacatcc ttaagtacta
     8461 tatcacggct tatttcatca tcaatatata cttcccagat acccaaatcc ctgaactttg
     8521 caatataaga attcttcagt atgataccgg cattcagcaa tatatttccg tcattgctgt
     8581 acagagtcct ggcaagaacc attccttctt ttaccttatc aaccggtaat tttctcatac
     8641 atgcatcatc tccttacggc gcagcaagaa taatgctctg tattattccc tgtgcctgtc
     8701 cctgagattt tccgctattt gctccagctt tctcaatcta tggtttactc ctgactttcc
     8761 tattgcaggg ctcatcattt cactcaattc cttaagggtt gcatccctga attccagcct
     8821 caattctgcg acttcagcca agccctcggg aagcttgttc aatcctattg tgttcttgat
     8881 gtattcaata ttttctatct gtcttaatgc tgcattcagg gtcttgtcca ggtttgcagt
     8941 ctcacagttc actatcctgt ttacattatt ccgcatttcc ttgtatatcc tgatgttctc
     9001 cagcttcagc agagcattgt gagccccgat tatgttcaaa aagttcacaa tgttctcccc
     9061 ttcttttata taaaccacaa agttgccttt cctcattaca acctttgcac cgagctgata
     9121 gtgattcatc aaatctttaa tatcttcggc aaaactctca ttatttgtta ccaattcaag
     9181 gtgatatgtc ttttcgggat cgcttatgga tccgctccca aggaaaattc ctctgagata
     9241 tgctttcctg cagcaagcct tctttatcag ataatgcgga agcttatgat tcaatttcat
     9301 acccttatca ctttcctgca atatccctgt catcttgagt atctcgtgag aacctatgtc
     9361 agaatcgata tataaggtgt agttgttgtt cttcttaaga tgcctgtttt tcctcacaat
     9421 caccctggtg ttgatatcaa aacagttctt cagcagtgag aacagcatcc ttgctattga
     9481 ggcgttctca gtggaaagtg tgattcctat cctttcaact ccatgtattt gcagggttcc
     9541 ggtcatttta ataagtgctg acagcagtgc aagctgacag cacttattag tgactgcaat
     9601 cttagatatc tcattttttg ttacagaaga aaaggtcatg ttaaacagcc cctctttatc
     9661 ttactattcc gaacttatta aaaggccata ttctgaatac tgctttcccc acaatattat
     9721 cacggcttac aaagcctacc gtatccgatc ggctgtccct gctctgcatt ctgttatccc
     9781 ccattacaaa gtacttctcc tccggcaccc tggacttggc aaagccgtta tcccgggtca
     9841 gtgcatctgt ataatcagga ttcagttcct ttccgtctat atatactctg tcatttctta
     9901 tatcaatgac ttcatttgga agccctatcg cccttttaat aagatattta tcctccaact
     9961 catccttaag taccacaata tcgcctctgg cagggtcgtt aatcaggtag ttcacctttg
    10021 ctattacaag tatttccccc tcataaaggg tgggttccat tgatacctga ttaaccagtg
    10081 ctatctggaa cacataggtt tttaatataa aggcaatgac aaatgccgat aatatcagtt
    10141 ttacccacga tattacctct ttttttatat tttcgtccaa tactatctcc cctttgttaa
    10201 ttacttctgt tcttcattca gcagcttgaa tatctgctcc gccagcttat tgtaatcatg
    10261 cctcagatac ccgttctcaa tgtttacaaa gtctccttca atgactttta tccccatatc
    10321 agctattata tcataatcaa tcaaaaccgg agaagcgtcc tccgttttgt atttttccaa
    10381 aagggcttgg gggatttccc ctgtattggc aatggcataa tctatcggag ccctgttccc
    10441 atgataattg attgccttaa tgtgctcaga aaggctgtac cctattgttt ccccctgctg
    10501 tgtcataata ttgcatacat atatcttcat tgctttggtg ctgttgattg cttctgcaat
    10561 actcttaacc aaaatattgg gtatgatgct ggtgtaaagg cttcccggcc caaatacaac
    10621 ggcatccgcc tcttgaatct cccttagggc ttccggcata ggcatgcaat tgcccggctc
    10681 cagaaagacc tttcttatcc tcttgttcaa agcccgctgt ttgatgggta tttgtgattc
    10741 gcccttgata atggtaccgt cttcaagctc tgcatacagc accacatctt ccagcgtcac
    10801 cggcagaacc tttcccgtca cagccaatac actgcttact tccctgacag cagcgtcaaa
    10861 gcttccgcaa atgtcattca ttgctgcaat aaaaagattc ccaaagctct gcccttttaa
    10921 tttaccttcc ctgaacctgt attgcataag ctgctccatt atcggttcga tattggcaag
    10981 ggccactatg cagtttctga tatctccggg aggaagaatt cccatatcct ccctgagcac
    11041 tcccgaacct ccgccgtcat cggatacagt gactattgca gtaatattgg atgtatgttt
    11101 cttcaatcct ctgagaagca ccgaaaggcc ggtacctccg cctatcgcca caatcttcgg
    11161 gcctcccggg ataatcccct tcccatgtaa tgtattcttt acagaattgt ttttcaaaag
    11221 tatccccctc gtattgtgtt ttattatcta tatctttcct cttcaatatc cctatgctcc
    11281 actataactc tatggtttgc ctccttcagc ttattgctca gcatattggc catggtaacg
    11341 gaacggtgcc gtccgcccgt acagccaatt gaaattacca gctgagattt cccctcttta
    11401 atgaaatgag gtatcagaaa ctccagcata tcgtttagtt tatccatgaa tcctgctgct
    11461 gcaggccact gcatgacata attccttact ccggcatccg ctccgctgaa tttcttaaga
    11521 tcctccacat agtacgggtt tggaagaaac cttacatcaa ataccatatc ggagtccagg
    11581 ggtatgccat acttgtagcc aaaggaaata acgtgtatta tcagcccttc cgatttccct
    11641 ccctcaacaa atagatgtgt tatttcctgt tttagttccg aaggactcat attcgtcgta
    11701 tctattatat tcgtagcttt ggattttatt tcctgaagct ttcttctttc ggcttttatt
    11761 ccttttatta tccggcctcc cacagacaga ggatgcatcc ttctgcttga tttgaacctc
    11821 tttatcagag tctcgtcgga agcttccaga aacagtatgt cataggtatg gcctaattcc
    11881 ttaagctcgt caaggcctac gggaagctga tccaggaact tgcctaccct tatgtctatt
    11941 actatggctg ccttgtctat atggcccttg ctttcagagc acatttcaac aaacctgccg
    12001 agcaaggcag gaggcagatt gtcaacgcaa aaatacccca aatcctccaa aaactttatt
    12061 gattggcttt ttccggcccc cgataaaccg gtaacaatta aaaatttcat tgacatacct
    12121 cctggattat tttacggcat ttataatctg atcacaggtt aagccggctt ccatggcaat
    12181 attcaggcca ttttgcaggt tgcccacatc ctccggagca tgggcgtcgc tgttaattac
    12241 aaactctgcc ccttcttccg ctgccgcaat aacatgcttt gctttcatat aactgctgtg
    12301 ggaatttatt tcaagagcgg tgcctgcctt ggcggcttct cttgcaacaa gggaaatatc
    12361 aagcggaatg cgggttccgg gatgtgttat tatatctatt ttatgctttc ttattgccct
    12421 aatcattgat tcggtgtttg cttccttaat tccttccgta cctagatgaa atatcttcga
    12481 caagtagttt ctgccataga tattcacagc atctccggct gtcttcggca ataccatcat
    12541 atgaaagccc accagaagta tatcaaggta tttgcgtata ctgtcatcca tatcaatatc
    12601 cccggcggtg cttatgatat tgcactccat acccatgagc acctttatgc cctctgtatt
    12661 gtaatttatc ctgtctattt cgtccctcat ttttgcaata tccttgattc tgacgccata
    12721 ggcaaaatgc cttattccgt ggtctgtaac tgcaatctca gaaagcccct tttccctcgc
    12781 cgccgttaca ttgtccagta tacttcccgt accgtggctg tataccgtat gggtatgata
    12841 atctgcaaaa agctttatca agtacttcac ctctgatatt aataatatca ttattattgt
    12901 caaattgcca cttgtcatac taaggagaca cggggacgtt tctcctgtcc cacaaggaga
    12961 cacggggacg tttctcctgt cccacaagga gacacgcgaa acgtccccgt gtcctccgaa
    13021 acgtccccgt gtcctccctc acgtaaatcc cccggatttt actgcgtatc ttctcttgat
    13081 tctgttgccc aaagccaata actagtcgct gttatacaag gccagtgctc tgcaggatgc
    13141 atacgaagta agagcttata tagtatagca gggctttaaa gtgtaggggt tgaacagttt
    13201 ttggagttaa aaaagcatct atatataagt catagtatac ttatataaaa attgtaaatg
    13261 actcttatta gctgaaactt agaaacagtc gtatgcgagg catggacgcc gcgcaagctg
    13321 tcacggtcac ggatggcctt tgacagcgtt agacttttct taggttcagt gaattagaag
    13381 tcatcaattt ttatatagct atacgagact acatatagat gctttttaat ctgctaaaac
    13441 tttcctaata tcttgacctc aggctcaagc atcacattga acttctcaaa tactgtcttc
    13501 tgcacatgcc tgatcaggtc atacacatcc tttgccgtgg catccccatt attcaccaca
    13561 aaaccgcagt ggagggttga tatctgggct ccccctatag aaaatcctct caatcccgca
    13621 tcttctatga gcttgcctgc aaaatacccc tcaggcctct tgaaggtact gcctgcgctc
    13681 ggcaaatgca acggctgctt gcttttcctt ttttcagcca gttccgccat tttgttgtct
    13741 atctcgtcct tgtttccctt tttaagcctt atacagcatc tcagaactat gtgctttccc
    13801 ggttctataa tgctcttcct gtacataaat ttcatttcat catttttcag tctctttaaa
    13861 ttaagatcct gatccagcac ctcaacccac tcaacaatat ccttcatttc tccgccgtag
    13921 gctccggcat tcattgtcac tgctcctccg agagatccgg gaatgccgct tgcaaactcc
    13981 attcccgtaa ggccttcctt ggcagccatt ttggataatg ttgaaagcag aacacctgat
    14041 tctgctgtga ttatatcatt attgatactg acgcttccga acttctctcc gattttgact
    14101 accgcccctc tcagtccttt gtcggatacc aggaggttgc ttccgttgcc cattatatag
    14161 tatgatacac attgctcttt aaggtcttta attgccttta ccagttccgc catgtcatcc
    14221 ggttcaataa atatatccgc aggccctccc accttgaagg aggtatgatt tttcataggc
    14281 tcattatata gtatctgcat tgctgccatg tcccctttca gttttcactg ttcctccgcg
    14341 cttcttataa ttcccatggg tgtctgctcg cagcattgcc ccagtggatt atcccttaat
    14401 atacctgcga gggccttcct gtcaagggat gattttgaag ttcccagtat actcccgccc
    14461 aaatcattaa tatctactat tgcgacctcc gcttttatgc tgtcgctgat gctttttgcc
    14521 acctgatccg ggttctcggg ccccagtaca acatatctgt tatacggagg cagagtgttt
    14581 ggagtaggcc cgtctatgga agaagcctta taaccggcta ttctgtagaa atctccctta
    14641 cgtccgaaca gctttccgaa aaaactcaca acagctgcaa aaagtattct cagcaccccg
    14701 cattccctta atgccatttc catggtctca ggtattccca agcctattcc tgaaggagtt
    14761 tttgtaacgt atttggacag cagccttgcc aggggcctcg gctttatttc ttccagcgga
    14821 taggctctcc cctgggttat tgccacagct ttttcgctta taaatactat atcattctcc
    14881 ttcactattt cccgggcata catactgaca acatcgacaa tgctgtcatc ctttgttatg
    14941 acatgggtct ttacaggtat tcgcaaatat tttttcccat tgtattctat taccggctgt
    15001 ttttccttat tggtactttt aacctcagtc atacaatctc ctccatttgg tttgtttctt
    15061 atgtgaaatt tgtccatatt tttacagtat tattcaacgg atattttata ttatggggca
    15121 gcgcttcccg cctctgcagc cacaccctgc tttctccgct gttctatcag cttttccgct
    15181 tccttaacag cctgctccgg acttatattc ccctgcaccg cttcctttat tcttgtctgc
    15241 agaataaggt cgatttcact ccagttgtcc gccttgggaa ggtgttccgc aaaatataag
    15301 ttctgctgta tagtatacat ctctttgtca ttttcgtaca gatatttacc tgattttctg
    15361 acgggaaagt aacctaaata ccccaactcc ttttgtgatt ctttggaggt gatgaatttg
    15421 atgaaatcaa tgcatacctt cttcttggcg gcatccttct gcttgaaaac ggcataagaa
    15481 cacacagttg aacttaatga cagaggcaca tccgactttc ctgtcggaaa cttggccaca
    15541 gcaaagttaa ttccgttgtt tccctggata ttccgcagca gcggcaccgt ccatgatcct
    15601 cctatgtaca ctgccgtctt cccattcaag aaagcggacc atgcctgatt ttcattcatg
    15661 gctccgaagt ccgggtggga gactttgtat ttatgcttaa gatcacacag ctttgtcagg
    15721 cctgatacag cttcgggagt attgaagata tatctgcctg ttgtatcgtc aattatctgt
    15781 gctccgtcac acattatgat tccgaaggta ttgtaataac ccggcccgat aaaggagttg
    15841 aaaccgtata tcccattata tgtaagctgt ttcaatgctt caacaaactg ctcgtaggtc
    15901 cattccccgt tctccggcag gctgacattt ctttcactga agatgtcggt attgagaagc
    15961 attgtatagc ctgtcatcat ccatggtatt ccataggtct tcccgttgta catcgctgta
    16021 tcaagtgtgc tgtcaatgaa gtctgccctt tcctccaagc tgatatatcc gtccaattcc
    16081 tccagatacc ctccggagat gaagaaataa tcgctgccta tgggtgcaat atccggattc
    16141 gctcctattt tggccgctgc tttcagaaag cccgggcctt cattccaatc aagctctctt
    16201 aactcaatat atactccggg gttctgtttt tcaaactgcc ctattttgct tttgatccag
    16261 ctgaaccgtg ttccgcttct gatatccagg cgcgggtaat cccacagggt tattacccct
    16321 gtccactctg ttttctcatg aaccggctcc aggcttttct tgtagtcctc cttcacatgc
    16381 tctaataata caggccccga aaatatataa agcgtcagaa tcccacataa aaaaagagta
    16441 aatgctttcc taagggtcat ttgtatccct ccataccggc ttttactcta tgtatattca
    16501 cttataccgc tcaggtattc ctcgccgtca aagtaccgta ttacacggaa cacaccggtt
    16561 tcccacggag tcgcttcgcg atttcacggt aacttatata tattgtggat acattagtaa
    16621 atttcgatac ttactttgct ttcgatgatt attaatgtat cataatattt tccccgccgt
    16681 gtactcagtg gatttaagtg ttttcagcgt tacccgttcc cccgtaactt aaaggcttac
    16741 tttcttacct ttcttgttca gatagtcaat aagcttcaag gggtcggtat ttatcaccag
    16801 agcctcaggc atttccagtt cctcaaatat ctcttcaatc ttgttaaact tccctacatc
    16861 atagcagata tgacagtcac ttcctgcggc cagtaccaca cccatctcct ttgctttagc
    16921 tgctataatc ctgcaatttt ccgagcttcc cttacggctc cccaaaaagg agctgttatt
    16981 tatttctatc aatgttcccg tatccttggc acattgaagc agcctgtcta tatcaacggg
    17041 atagtacggg ttcccgggat gggctataat atccacaaag ggatttgaca ttgccccaat
    17101 tagtgccctt gtattctctt ccacagttcc tgagtcaatg caggcttcat gaagagaagc
    17161 aatgactata tcaagttttt ttaagtattt ttcgggcaca tcaagtctgc cttcaaaatc
    17221 cattatgttg gcctctacac ccttcagcac ccttacaccc ttgatatatt caggtataac
    17281 tctcatattt ccaaaatgga ataaatcggg tcccccctgc atatccggtc cgtggtctgt
    17341 cattgctatt aacttcaagc ccatatctgc cgcttcttct gcaatttctt taatggtact
    17401 gtaagcatga ccgcttgata tggtatgaca gtgagtatca agtaataatt tcaacaaaac
    17461 accctctttg ttattgttcg tctttttcta ccttatattc cggattgtac cccgtcggtt
    17521 catccggaat aataagcgcg gcggctacat aggcaaatat cccggggaaa aatcctgttg
    17581 ctattgtaag ggcaacccat ataagcctga ttatcgtaac atcaatattg aaatagtcct
    17641 ccaagcctcc gcatacacct gatattgttc ttacagttct ggatttatat aatttcttat
    17701 acatattatc tttacctcct ggttttaatt tgtcaaacat gcttcaatga tgacaggcaa
    17761 gaattgcccg ggatagacat gttcgctttt tggttttcat aatatattac gaaacatttt
    17821 tgaaaaagtt ttatgaatta aaaaaattat atacaacttc tgcaactttc ctgttcatcc
    17881 cctgtacttc ctcaagctcc tccaaggatg cctttttgat attcttcaag ctgccaaaac
    17941 tcttcaacag tgcttttctt ctggcaggcc ctattcccgg tatgtcgtcc agagcagact
    18001 tgacgcttgt cttgcttcta aggctcttat gatagcttat ggcaaacctg tgtgcctctt
    18061 cctggattgc ggatatcagc cgaaaaacct tggagccttt cggcagttcg atttccctgc
    18121 cgctatatat cagccccctt gttctgtgct tatcgtcctt taccatcccg caaacaggta
    18181 tatttatatt caattccctg agaaccggca gcaccgcatt gacttggccc acgcccccat
    18241 ccagcattat cacatccggg aacaccgaga atctgcccag ttcaaagtct ttttcctctg
    18301 cttcaagttc ttctctctcc ttaaggccat gggcaaatct tctctctata atttccctca
    18361 tgctttcata atcgttggcg ccttcaacgg attttatctt gaatcttctg tagtttctgt
    18421 tattgggctt tccctgctca aaaactatca ttgaaccgac attcaatact ccctgaatat
    18481 tagaaatatc aaaagcctca atcctttcag gtacagcatt gagctccaga atgtccttaa
    18541 gttccctgca agctcccatt gttttctcgg tgtcattttt ttgcttttct atcagaagcc
    18601 ttaaggtatc ttccgcattc ctgcctacca tttcaataag cttttgcttt tctcccctct
    18661 gagggacaac aagcttgacc cggcctccct ttttatcgct gagccatctc tcaataatat
    18721 ttatctcgtc aatttcctcc tgaagtatta tttctctggg tataagcgca tctgacccat
    18781 agtactgttt aataaagctg cttaatatct cctttgagtc actaagcttg gtatcctcaa
    18841 taagaaaatg ctctctgccg ataagctttc ctcctcttac aaagaaaacc tgtatgcaag
    18901 actccgattc atttaacgca aaggctatta tgtcctggtc tatcaatgct gaagatatta
    18961 ctttctgctt ttccgcaatc tttatcaggg agttcatatt atccctgatt tcagctgcct
    19021 tctcaaaatc cagattatct gccgcttgat gcattttgtc ctccagcccc ttcaatactt
    19081 cctcctgctt gccctcaagc atcattatta ttgagtcaat aagctccctg tattgttcct
    19141 tgctgacatt tccccggcat gggcctatac atttttttat atgatagttg aggcagggcc
    19201 tttcctttcc tattgccctg cttatgtttt tattgcaata tcttatggga aaaaggcctt
    19261 tcaggaggtc aatggttctg tttacagcgg taacatcggt gtaaggcccg aaatactttg
    19321 ctccgtcctt ctttatctct ctcacaataa taagcctggg ataatcttct tttactgtaa
    19381 tcttaatata ggggtactgc ttgtcatcct taagcatcac attatattta ggcctgtgtt
    19441 tttttatcag gttacattcc agtataaggg cttccagctc cgaatcggtt attatgtatt
    19501 ccaggtctgt tatcctctca accattgcgg caacctttgg attctgattc tttgagttct
    19561 ggaaatactg ccttaccctg tttttcaggt ttattgcctt gccaatatat ataatctcat
    19621 tatattcgtc acgcattata taaactccgg gtttttccgg gagatttttc agtttgtctt
    19681 ccaatgaaat catcagtgta ttcctccggt tttgaatagt gtatgcttct attatactac
    19741 aagtttgctg aaataaaacg aggcaaaaag atgtccccca cttctacaag tggcgtgtac
    19801 cgctttcgtt acccaacagg tggtgagcat atcgcccgcc tcgttgaaac gctcgggtag
    19861 gtttttgcaa gtgtgcaaaa accctcattg aaatcttgtt ataaaaagga gctgcgggac
    19921 gtttcgggac atggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc
    19981 catgtcccga aacgtccccg tgtcccgaaa cccgtgtcct cctatcccat tattacctca
    20041 atatcatgag tcaattcatc gttaaacacc ggtattaaat tgcttgcggc agtctttccg
    20101 tctaccctca ccctttttat ccctttgctg acatgctccg ggtttttcac gcttatattg
    20161 tatgctgtat tcctaaattt tcttataacc ttgaagccgt cccattcttc aggtatgcac
    20221 ggatcaatta taagcccttc aaagtcaggc cttataccca gaatccattt tgtaaccgct
    20281 tcgtaattcc atgcggctgt tccggtcagc caggaattct ttgcctcccc ggggtttttg
    20341 gcatcctttc cggcaatcat ctgtgtatat acataaggtt ccatcttgtg aatatcgctt
    20401 atatcttcaa tatatgcggg agcaattttc gaatagtatt caaaagccct gctgcccctg
    20461 cccatccttg tttcggcaat cataatccac ggattgttgt ggcagaacac ccctgcgttt
    20521 tccttcaatc ccgggggata agaagatatc tcgcccagat tgagcttata ttcactgtag
    20581 gctggatcct gaagcaccaa accatacccg gtgttcagcc tttcgtgaac cgagtccaat
    20641 gccttcagcg ccataccgtt ttccagccct atatcagcca ttacacaaat gccctggggt
    20701 tcaataaaca attgtccttc ttcattctcg gcacttccca ctctattgcc gaaataatca
    20761 taagccctga gaaaccaatt ttcgtcgcag ccatacttca taatactgcc gcgcatttta
    20821 tccacctgct cttctatgcc ggatgcttga gcatcatatc ccagaagcct gcagagcctt
    20881 ccgaattccc tgccgacgta tacaaacaac cccgcaatga ataccgattc ggctgtcttt
    20941 ccatttctgt tgctgcaggt ctgaaaggat tcatccgggt ttatggaaaa acagttcaaa
    21001 ttcaggcagt cattccagtc ggcccttcct attaacggca gtccgtgggg ccccaggttt
    21061 ttcatcgtat attcaaaagc cttctccaaa tgttccttca gcgtgcccag cttatttgcg
    21121 tcgcaattgt agggcatttt ttcatcaagg attgaatagt cccccgtttc tttgatatat
    21181 gttattgtgg ataatatcat ccataagggg tcgtcattga actctccgcc tatctcatta
    21241 tttccttttc ccgtaagagg ctggtactgg tgatacgccc ctccgtcctc aagcattgtt
    21301 cccgcaagct ccaatattct tgaccttgct ttatccggaa tcatatgcac gaatcccagg
    21361 atatcctgat tggagtccct gaagcctatc ccccttccga ttccggtttc aaagtaggaa
    21421 gcgcttctgg ataaattgaa ggttatcata cattggtagg gattccaaat gttcaccatc
    21481 ctgttcagct tctcctcgct gctgagcagc tgatatccgg acagcagctc atcccaatac
    21541 tcccttagtt cctcgaaggc tctttccaca taactgtcat ccttatattt ttctataagc
    21601 tcatttgcct ttgatttgtt tatccgccct tttgactccc atttttcgta atccccgttc
    21661 tccccatagc ccagcacgaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnccttttga ctcccatttt tcgtaatccc cgttctcccc atagcccagc acgaaaagaa
    21841 gtgttgtctg ctcaccgggc ttgagtttta tcctgatttc atgggaagca atgggattcc
    21901 atccctctgc aattgaattc cgcgatttcc cttcgaaaac tgcctcaggc tcatgaagtc
    21961 cgttgtatcg cccaaggaaa gagtccctgt cggtatcaaa accgtcaatc ttcctgttta
    22021 cgctatagaa ggagtagtgg ttcctccgct ccctgtaatc agtcttatgg aatattgtgc
    22081 ttccctccac ctcaaccgca ccggtactga aggtacgctg gtaattggtc atatcatcgt
    22141 aagcattcca caaacagaac tcaataaagg aaaacaaagt gatttcctta tcctcttccc
    22201 ctctgttcac cagacgaaga tgatgcagct cagcattgtc cttaaggggc acaaaataca
    22261 gcacttcact ttcaagccta ttctgaaggc attttatttt tgtgtaaccc aggccatgcc
    22321 tgcactcgaa gctctcggca tcattcctga ccggcatcca ggtcagtgaa taaacctttc
    22381 ccgagtcatt gacatacaga taccttcccc cattatccca cggaatgctg ttgtacctgt
    22441 atctggtaag ccttctgagc cttgcatcct tatagaagca gtagcctccc gccgtatttg
    22501 aaatgagtga aaaaaagcca tcctgcccca aataatttat ccaagggaag ggagtctttg
    22561 gtgtatttat gacatattcc ctgttcaagt catcaaaata tccgaatctc atctgtcttc
    22621 ctccgtcaaa aaaaggtctc ttcccattaa aacttctata ttatgcagcc tgttgtccct
    22681 gaagcatggt aacacatttc tgaaaaatac tgttccatcc acccatattg cccttattcc
    22741 tttggaaaca ccctcaggat tcctcactct tatatcatat atcgaacctc tgaagtgctt
    22801 tcttacccta aattccttcc atgcccttgg tatgcaagga tcgattgtca tgcccttata
    22861 gtggggccga actcccaata tatacttcac ggctgcctga taaacccatg aagccgtacc
    22921 tgaaagccag ctgtttcttg caagtccgaa ctgcggatgt tcatcgccca gcatattctg
    22981 gggatagcag taaggttcgc attggtattc atctatacac ccattcttgc aggcaggatt
    23041 tatctggcta tagtattcaa atgccctgtc gccgttccca accattgtct cagctatcat
    23101 cacccagggg ttgctgtgaa ggaatattcc cccgttctct ttagtcccgg gcggatatgt
    23161 agtaatgccg cctttttccg ggtcatagcc tttgtatccc ggatagctta gttttattcc
    23221 gtttctggta ttcaatatgc tcctgactga gtcaagggat tttaaagccc tgtcttcacc
    23281 ggcaaagcct gatatgactg cccaggattg ggcattcgca tatatttgcc ctgagctgtt
    23341 cactttagag cctatagggt ttccttcata gtcaaaatag ctgatatacc attctccgtc
    23401 ccagccgcag ctgttcacac gctcccgcat cctttgataa cgaccccggt acatctcctt
    23461 aagctttttc tttccgagga aatccataag ctctatcatc tcagaaaggc acttcccgta
    23521 caggcaggcg gtaaaaaccg attcagctcc cgccgggaga tttacggtat cgttccagtc
    23581 tgcaaaacca agaaggggca atccgtgcct tcccaagtca tttccggtaa atccgactgc
    23641 tctttccagg tgctcaagta cagtgccttt ctcaatcggc atcccgcttt tgtccttttc
    23701 ataaaaaggc actgttttat tgagaaaatc catatttccg gtttccttga gataggcaca
    23761 gacagcaagt actatccaca gatggtcatc actgtaatac ttgggcctat cctcaaaatc
    23821 ccttgaatct ccttcatttg caatcattgt agccggattg aactgatgca ttgcagagcc
    23881 atttcttttc tgcacccgga gaagcttttc tatcagctct ctgccttcct cgggagcact
    23941 gtccattatt cccattacat cctgggcgct gtccctgaag cctattcccc tggagccata
    24001 gccaagctga tacagggaca ggtacctgga ccagtttttc gtaatatagc attgtctcgg
    24061 gttgtgtatg ttcagcatgg tattcatagc tttatcaggg gtttctacct gcagcttcga
    24121 aagatattcc tcccagaagt atttcaattt ttcaaattcc tcattgaccc tttcttcact
    24181 tctgaagctt tccatgctct ctttttcttc tgtaatgctc ctgcactgtc caagctgggt
    24241 gatgatccgg cgggattctc ccggctccaa tatgccgata tgatgcatca tcgcagctat
    24301 attatcgccc cgaaggactt catggttgcc aagttcgctg ttattaaggc tcccggggct
    24361 ctcccaggtg ccgtattcgt tatccccgag aaaatacctt ctgtccgttt cataggaaga
    24421 tgctgcatgg tttgaagtga agaagttgat ccttgtgtcc ttgttcatgt atgcatattg
    24481 tataagcgtc ctgtatcctc tatgttcctc aatacattcc gaacgcattg tctgaggcac
    24541 ccaatcggcg ttaatgagtt gtttcagcgc ttcaaaatga gaatactcaa ttaccggtat
    24601 gacatcaagt tccacgggta ctgcccttag attggttatc ctgatgtccc ttatctccct
    24661 gtttcctccc aaaggtacga aaacaagcgc ctctgtcctt atatcgtaga attctgatac
    24721 aatccttgtg tagcctaatc ccacatggca ttcgtacatc tggtattgat ccagggtagg
    24781 cacaaaatac ggagaaaaca aggtgtatgc atttttaccc cttatcctta catacagcgt
    24841 ttccccctta aattccgacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24961 tatgcatttt taccccttat ccttacatac agcgtttccc ccttaaattc cgacgccgga
    25021 agctgcggta tatattttat tattctgttt actgcagggt ctcctttgca tatcaatgct
    25081 cctcccgtat ggtcgataaa tcctccgaag tccaggctgc ctatataatt tatccatttt
    25141 acgggagttc tcggatcagt aatgacatat tccctgttaa tatcattaaa atacccatac
    25201 tgcataacaa ttctcctttt atttcaacgc ccccggttgt tatacagctc cggggcgata
    25261 tttttccata actctgccga ctaccaaata gatagcaaaa gcagtaaagc tgataaaaac
    25321 caacaggaac aatccgggta ccgttcttgc gattaatgcc gcggcagcaa caattgccag
    25381 agttccgaag caggagaaca catgctttat tcccaatacc caagaagctt ttattattcc
    25441 tatggtgctt gattcatacc ttgaaagcag tggaaaaaca aaaaccgtaa ttattaccac
    25501 ctgaaccagt accggcagct gaactgccag catcaatccg cccatggagc catatttctc
    25561 caggaagctg aggttggtgt atatcatgac atatgccgca catagtatca tccatatgca
    25621 ggtagcctgt ttgaaatttt ccctgaagct tttataaaag tccttgattt catgtacttc
    25681 ttcttttctt gcgatttttc ctataacgta gtacaaagca gtagttgatg ctcccactgt
    25741 tatcagcggt atgctggtta tgcaccagag caggttaagt ataatcatct ggtatactgc
    25801 cgtcattatt ttataaatga ttccctcggt attgaacaac catttcataa ataccgctcc
    25861 ttctgagact tgttttatgc ttaaggctaa tactccatag gagcatcagc cttaagctta
    25921 tgagtatcta ttgcgtcagt ctatttcttt cccatttttt cagcccattc attaagctgt
    25981 tcctgctttt cagctattac tttgtcaatc cctgctgcct tcagctcttc aaggaacttc
    26041 ggcacattta cttcagggtc tattgagcct gtttcaagag ccggtacata tttatcccac
    26101 aggttgttca ctgctgcaat ttcatttttt accggcgagg ggtcaaagtt aaagcccagg
    26161 gtttgagttc ccgcagcctg ctgattgaat tcctcaaact gctcccactt cttcgggtct
    26221 tcgctctcat acaggtaatt gatgaactgg ttgccgaaca tccagcctgt tcccggatta
    26281 tatttcgtct ggtccggacc ggcctttatg acgctgtcac tgactttaac gtagtgcttg
    26341 ccttcgatac cgaagtttat aaggttgttg aggtatgggt ctgtgttaaa gagttcaagg
    26401 aacatcagtg ccctttcagg attctttgac gtacttgaga tagcctgtaa ggagcccatt
    26461 gtatccctgc ttgaaattac aggcggggtt atatatacct gtacccactc aaatccgtgt
    26521 gtaagtgaca tctccgcatc ttttcccggc tttagggatt tggttgcagc aaatattttg
    26581 cctgctgctt catcggcatt gtagtcggtt acagtagcag catccttcct gatatagcct
    26641 gcctggaaga atttccttat agtcttaagc agtgccattg tttccggttt ttcaaattca
    26701 ttgaatacta caaggtcctt tgaatcgttg tatacaactc cggggcactt gtcatcacct
    26761 ataacatccc agtccataag cttatacggc gattcgccgt tcaatgcttc aagcccgtat
    26821 acattgggtt ctttttcctt tattaccttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnt tctgcatgta gttgtttgcc caggctgcag taaagcatat gtcaaactcc
    27001 tgccttgaag caatcatctg cctcagtttg tcgtcatact ctgctcccca ctggtagcac
    27061 tgaagcttta cagtagcatt gatcttgtct ttgatatact cgttgatctt ttcctcgatc
    27121 agggatacat cctcctgctg accattgcct ataaagaacc aggtcagttc aacaggttcc
    27181 aatgtagttc ccgaacccgc ttccgccggt tcaggtgcag gagccgcagc cggctttgca
    27241 cagcctgtta ggctggccag cgcaaatacc agcaccagtg taagacatag aagttttccg
    27301 gttttaaaca tactcttctc ccctttaata aatttttata tgcttgcctg ttaaggcata
    27361 agcccttatg attaaccctt gattgatccg atggtcaggc cttctacaaa atacttctgg
    27421 aagaaggggt aggccaatat tatggggcct attgcaacta cagccatagc cattcttgct
    27481 gtctgagacg gcaggttcgc catgatctgg gttgcattgt ttatagagcc tgcattattt
    27541 gaaaggtata ttatcgcttt atccaccctg tacatcaggt attgaaggtt gaaaagcttc
    27601 tcattgttta tgaagagcag tggtgcatac cagtcgttcc aataagtgag tatattaaaa
    27661 agccctattg tcgccaatac aggtttggca agaggaagca ctatccatac aaaggtctgg
    27721 aactcaccgg ctccgtcaat ccttgcagct tcgattattc cgtccggtat ggtagtcatg
    27781 aagaaggtcc tcataatcag cacataaagg ggggtcatca ggtagggcag tataagtgcc
    27841 attatagtgt catctatatg gaggacattc acatatacca tataccaggg aacaaggcct
    27901 ccgttgaaca gcatcgtaag aaaaactata aatgtaaaaa cattcctgta tttaaagtct
    27961 tttctggata tcggatatgc gtacaaagct atggtaacaa ggcttaatgc actgcccacc
    28021 acagtaacaa atacagtcac tccataagcc ctcagaatta ccattatatc cttgtaaata
    28081 tacttgtagg cttccaggct caggtttctg ggaaaaaggc tgtagccgta catggtaacc
    28141 gtcttctcat cagttatgga agcggagaaa acaattaaaa gcggaagtac acaggctatt
    28201 gtacagagta tcattacaat attgagcgct atgtcagctt tccggttgat ttttatgaaa
    28261 tccctcaact ctcacgcctc cttaaaacaa agaactgtct ttgtcgattt ttcttacaat
    28321 atagtttgcg gtcatcacta ctatgaatcc tactactgcc tgatagaacc cggctgccga
    28381 tgccatccct atgtctccgg tctgcctgag cgccctgaat acatatgttt ctataacatt
    28441 ggtcacatca tacagcgggc cgctgttcct gggtatattg aagaacaatc cgaagtcagt
    28501 gaagaaaatc cttcctactt ttatcagtgt taatacggta attactggca taagcatggg
    28561 aaccatcact ttggttatct gctggaatct ggaagcgccg tcaactgccg ccgcttcata
    28621 caaggatttg tcaaagccga tgattgcagc cagatagatt atgctgtcgt atcctgtcca
    28681 tttccacgta tttaccagta taattatcaa aggccagaat cttgggtcta cataccattc
    28741 aacaggctgc ataccgttac tgataagaat cttgttgaca aaccccatgt ctgagcttaa
    28801 gagggcaaac accagatagc ttatcacaac ccatgaaaag aagtatggca gaaacatcac
    28861 actctggtag aacttggata tgcgcctgtt caggagttcg ttgaagataa tggcaagtac
    28921 aacggcaaca gttgctgcta ttactatgaa tgcaacattg tacagaattg tgtttcttgt
    28981 tatcaggaaa gccgcttttg agttgaaaag atattcaaaa ttgttgaacc ctacccagtc
    29041 gcttttccag aagccttttg catagttgta atccttgaag gctataacaa tgccaaacat
    29101 cggaaggtaa ttattgacca gcagcaccag caggccggga agcagcataa ggtacaacac
    29161 cttattcttt gccagttctt taagcaaggc aggtttttgc cttctcacag tcgttatctc
    29221 ggtgcccgaa gccctttgca tttcagccac aggaataccc ctcctctatt tcagatcctg
    29281 tcgggtttaa cttcataccc caataatata ctgcggcagc tctccattaa atggagcatc
    29341 ttgtgaattt gttttactat gttgttcaaa acaaaagtgc aacattttcc actaaaagac
    29401 gtaaaaatgc aatatatccc ggctcatatc gtannnnnnn nnnnnnnnnn nnnnnnnnnn
    29461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29521 nnnnnnnnnn nnnaagagct ttgagaagta atgtgcatct ttgatcccca caagctctgc
    29581 tatttcatac gtcttgcagt tggagtcctt caggtactcc ttcgcctttt ctatccttac
    29641 ctcattcagg tagtccacat agttctttcc caattccttt gtgaacatcc tgctcagata
    29701 gtatatgctc acataggtgt gctccgcaac ttcattcagt gtgatgggtt cgttatagtt
    29761 cagacagata tagtccatag ccttttgcag tgtggaattt attgtcttcc tgttgaagct
    29821 gtttatccgc tctgctatat ttaatgcagc ttcctccaat attccgttta tttcaatgat
    29881 actttcgcac tgatcaaggg cggcaaatat ttctgtaatt cttctataat gaaagctctc
    29941 atcattcagc ttcattgttg taggtatgct gcagacaagg tttattagat tccaatagaa
    30001 cttctttatg ctttccctgt caagcttgca ctcgttcacg cattttaaaa tatccttcat
    30061 caccagggac acactgtcct tgttgcctga cttgaccatt tgaacaagag atttttcata
    30121 tacctccagg gccgaataat cagcagactt ataaaaggat ttcaaatctt catacagtat
    30181 tattgtattc ttgcccatat aaaacttata atccagggct tcaagggcct ctcttgcctt
    30241 atctgcaagc tcaagggccc cgttcccctc cgagcttatg gatattgtga cggtaaagct
    30301 gaagcagttg cgcaccagct cctgaaggct cacagcatcc ttgtagagag agctgaacat
    30361 gtctccttcg gattcttcat tgggctgaat tacgaataat atggtcctgc tgtttatagg
    30421 cacgtctgct gtcttgaagc ttgttccgag tatttcctca aaggtgttga tgataccgaa
    30481 ctggtacaga tgcttttcat actggctttt cgcattttcc tgcccctctt cgtcggtttc
    30541 cacagccatg attttgaatt tatcaatagt aagcccgtaa agattaagtt cctccaaaat
    30601 ctcatacttt tttgtattta tcctgaaaag aatatcgtac agcagcttct gcctgagtac
    30661 cggtatttta ctcttaaaat gcttcttcag gttattgagt tcttcctcca tcttacagct
    30721 gcgctgcagc tccgctatcg ccttttgaac tatggcggtc aaatcctcta tttttgacgg
    30781 cttcaacaaa taatcaaagg cccccagcct tatagcttcc tgtatgtatt caaaatccct
    30841 gtagccggta agtattatta tcttgctccc gggaagcagc tgcttggtct ctttaatcat
    30901 agagaggccg tctatttcag gcatattaat atcagttatg agaatatggg gacgcagctc
    30961 ccttatcatc ctgatgcctt ccattccgtc gccggcttcc ccgcatacct cgcagtccaa
    31021 gctcttccaa tcaattatat ttttgaggcc ttttcttatg atagattcat catcaacaat
    31081 cagcacctta tacattgccc ttgtctcctt cctgaacctg ttcatcagga atccttacag
    31141 tgaccttggt atattcatca tagctgcttt ctattttcac tccgtatgag cttccgtaca
    31201 acagctttat cctcctgttc acgttttcca ggcctatact ttttcttgcc ggggctgtac
    31261 cgttcccctc cagtatcaat tcatcctcct gcagcctttt gttcagggct tccagttcct
    31321 cagccgtcat tcccattccg ttgtcctcaa cctcaaatac gatatgccct tcctccctga
    31381 atgcctcaag cctgattact ccttttctcc ggcttttccc gatcccgtgc tttacagcat
    31441 tttcaaccag gggctgaatc agcagccttg gtattttctt gtccataagc ccttcatcca
    31501 ggatttttat tacttcaagc ctatcttcat atctgttctt caataccgtc atatagttgt
    31561 ctatgtattc cagttcttct ttcagggata tggtcctgtc ccccctgcct atgcttccgt
    31621 ctataagctt tgcaagagcg gataccgttt cactgatctc aggcactccg ttaagccggg
    31681 ccatccaatt aatattttca agagtattga aaagaaagtg aggatttatc tgggcttgca
    31741 gcgcctttat ctcagcctcc ttaagagcga tttcttcttt atatatcctg ttaacgaggt
    31801 aatctatctt ttttgacatt ctattgaagc tctcactcag ataccccaat tcatcactcc
    31861 tgtcaagttc aatatcctcg tgcttattgc ccttctccac ctttttcatt ccctctacga
    31921 gcttgttgat gggagtaatt atatccaccg aagtaagcac gctgagcacc gatagaataa
    31981 taagcccgta tacaataatg agcagcaccc atctcttcaa ggtatccatt tcccgataaa
    32041 gctccttaag gggtatatga tatacaatct tccagctggg atcctcaagc agaacgtagg
    32101 atacaagcat tcctgccttg ttatccttat agaagcccct tctcccctga agctgctgcc
    32161 ggtagaagtt cttaagtatt ccggagttct cctgctgctt tattatttcc tcattattct
    32221 ccgagagtat tgaaatgttg ttcagggact ccctggataa atcggtatat accgactcaa
    32281 gatactcctt tttaatattt atggcaatga ggccgatttc attatagctg tccctgtcat
    32341 aaaccattcg ggtaacatat atttcgcttt cccggccctc ctgtttttca agaaaccata
    32401 ccggcctgcc cctgcctttt cgcgctgctt ctacaatgct gtcgtaagga agtatgaatt
    32461 ctatccttac cttcccattg tcagaatcaa agctgtaaaa cctcctgttc tttgtcacaa
    32521 ggcatattga ttgtatctca tttctggaaa gggttgcctg ccttaatatg cttcttattt
    32581 catttgccct gttgtatatg gttatattat cctcataccc tttgctgctc agtgcatcat
    32641 aaaccctgtt gtcataaagc agttcaaggg taagcccctg cataccggtt gacaggtcct
    32701 taaagcgcag ttctatcatt ttcagaatat ccagagaata attaatagac ttctgctcaa
    32761 ttatttcagc agacttctga taagaagtaa taccgataaa gaacagtggt atgactattt
    32821 gtatataaaa caacagcagc agcttgttct ttatagatgt attcttgtaa aacagcacga
    32881 atttgttaat aatactttcc attgcaaatc tcccttactg ctgtgccgcc ctgcgttcca
    32941 tttcttcaaa aacttcctcc ggtgtcatcc ttccctccaa gacatgggga aagcttgtta
    33001 caagcacctg ctcccacaga tttctgtcta taaagctgtc tgtaggccct accaattcca
    33061 ttgaccgggc tatcagttcc ttgccttttt ccataagcct gcccggttgt acactctgat
    33121 caggtatctt tatattgctt atgaagccgg tttcatggct gaacagcttc gctgcctcca
    33181 ctgatgtaag gtgtttgagc agcttgatgc acatctctct tttatccgga ttctcatagg
    33241 cttttctgct catatggaaa ttgccgtttc ccaaaccgta tattatgctg cttgtgtgtg
    33301 acttcccttc cttaaaagcc ggaaaaggta taatatctac cgtttcatcc tctgcgccca
    33361 gagaaccgag gccgatgaac caggagcctt gaactatcat agcggcttcc ttttcaagaa
    33421 agagattatc cctggtctta tccgcaatca caaaagcatc ttcgggaaat gcccccagct
    33481 catacagctc cttaacatat ttcattcctt caatatagtt ttcgtttatc ctgccttcct
    33541 tataggggtt ctcggtatat tccctgcccc caagcttcat tactatattt tgatacaaaa
    33601 atgtcccttc cggtgtggaa ttgtatgcaa tagggattat atccctctgt ctgaatgccc
    33661 ttacggcatc cttaagctct tcataggtct ccggcacctt tattccgtac ttttcaaaaa
    33721 ggtccctgtt cacaaacagc ccctcatata tgatttcgca gggcaagcca tagatcctgc
    33781 cgttaaaccg gtcatagctc catgcgtctt caccgaagcc ggcggcccat tccgggtctg
    33841 aagccagaag ctcagtcagg tcagccacct tcccttggtt tatcaatatc ttaatatccg
    33901 agccgggcca aagcccgaat acatcggggt cgctaccctg tgcaaaattg gttttcagct
    33961 tgaaaaggaa ttccgaaccg gacatggatt cgttgattac ctttactccc gggttttgct
    34021 cctcaaattc atccagcagc atctgaagct gctgggcttt cgtatcggtt cccccccagc
    34081 tgctgaggaa tcttaattca attgtttcct tctcaccctc gaagctgtcg gccggaatac
    34141 ctctgtcata cagcttaatt gtatacatga caggaataat tgccaacgca agaaatattg
    34201 ccaggtaata tatgtcggac ttttttattt tcatatattt caccccaaaa tgtgcattca
    34261 caaattgagt actaattctc taaatttacg taaatttcct gcaaaatttt gtcatagtgc
    34321 aatatattac aataacagaa caaaaaaaga ccctccataa gggtctatat gaagaatgcg
    34381 tcatagcaat gagacatggg gaca
//
LOCUS       DAOOAB010000025        33822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1672_length_33822_cov_20.918441, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000025 DAOOAB010000000
VERSION     DAOOAB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 33822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33822
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_33822_cov_20.918441"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..592
                     /gene="rrf"
                     /locus_tag="PKU88_07060"
     rRNA            476..592
                     /gene="rrf"
                     /locus_tag="PKU88_07060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            965..6391
                     /locus_tag="PKU88_07065"
     CDS             965..6391
                     /locus_tag="PKU88_07065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPX69073.1"
                     /translation="MQFINEQYKVVKEKSSDAYGTTYIVEDIQKDSLLKHLRIINLQN
                     ETRDFIDYMKNNFYNYSKYYHPYLIDFHFFNRIRLIDYKHTVLNHYYYTYDYFEGVNL
                     FDYCKGKDFDSILDLAAELCEAVKFLHLRGFLLCSVDSNDFQIVRDGEKGHLKILALP
                     YPRGTGSRIIINKKSTCFAAPELNKDESYSVLTDVYIIGSVISCMLKAFNHSEEKSEA
                     NHRAIMDIIKKCMAEKPEDRFQTIDQIIASINNYFGKEYRTINKQYIQAMPQYRINPS
                     SRHYLIDKILNNAKEHFFINRPNKVSLVVGLEGTGKDNFLEILSIKAQHEGFISVKTV
                     LNESEVLRFSVAETFVRGIIKYADKEIIDKYAEAVNNVMLTISKFKSISPKVEDEYFE
                     KGSKEKFIQKLSNFIEEATKKSHCIFIIENFQWIDEDSLSLIDGMLKSRNSSSAYIVL
                     ATDKETYSKNIKLKEYCRKLKKANLMNNTIVLKNFNFEETAGFIKLILSLDKVPHEFA
                     KIIYDRTKGSPDYIYDTIYMLFSNNNIYIDDKGCWVLDNVNYELLNVSYAEDMDMLNS
                     IYKLDSNYQDILKAVSIFDSAVSADIAEGFVEVKGERLVSQMNYLSYISIFVRKQNDW
                     GVSYSFSSLKLKESIYKSIPKELRRKYHEKASYILKSKLSDKNIKGEDELMRQMLRAN
                     WHLEVKEYISDSVRKMMENNSIYQTIQFLEHVNELLSKENVTEERISVCSILGELYER
                     IGEYSKAVYCFNILEDIAERKGNNCLLADVYINKYSLLYKLGDRKVSLKYLAYAKNLL
                     RTIDYKKGMYEHIIVMHRMMFHKRKFSSYIRILESALKEIDKEEYKFIYARMLGIYGR
                     LKAYKGEYDEGLIMLEKSIQILESIGNYPKMLYPLNSIGTIYYNHYTDIQKAREYYER
                     CLSISQRVGNVYYESISYNSIADQYRMEDKYSTALQFYQNSLKKAKQIRDKYTEFIIN
                     LNMSLTNIEIEDYKKVLLTLDDMEEEIANSRYSGDLMDYFYQCKAEFLYAMGEYERAA
                     EYAQKSVNMCITWGITENYEAHFMKLLSEIQLSGKLNHGRIKEILDKVFEVNWYKLGR
                     TACVKLAEIYISKGMRKKGLELLARGLRYASNIDTDMLRLRYDYVEALAKKGAERLEG
                     LTKLAGLMESAENNEIKWKIYKAIAEELMAQKNYREALKYLITSLSYLRKLVYNVPDE
                     YKIRFINSHDRNSVKENLCRAAELMTGNEPGEKAAKEYMTGNISLQEMDKYFDYTEYR
                     DMYRYKEADETAAGEDLDTSTRVKFLGKVQELVSRFSDDETANLKHMVDLFAEITQAK
                     NAFIATLQEDGSLNVIVSYNRYQETPFYKYLIEQAKQKRDSIIVNDTFDYNAKKGDIL
                     IPKDITAVFCIPIMIQKERDGIGIELVEERRRYKEQGDNPIIGYIYLDTDSIISNFTQ
                     ESSLFCMMAAKIAYVLTDNYNMKVMSTVDKLTRLYTRKHFESALSKELAYAEKEGGQF
                     SIIMADIDKFKSVNDRFGHQKGDEVLQNISSIIMSSVRKGDICGRYGGEEIIILLPGT
                     DAKGAYSVAEKIRKKIENAKLLGLNTPLTISLGISSYPEHGTWAKDLIDKADQALYHV
                     KESGRNGSRIYELNMSNSIKRIDRLAGIISGNIVEDQRKTETMLEILDLQRDSDKTLE
                     EKLFSFLGRIIEVSEAQTGGIFCIEEAGNEKRISRKILRKRLADKEEEVYYNEGIVQK
                     CMETGKGEYQIDWNSYPGIDILTGMPDWQSVIAVPMIDRGRIKGVTYLSVPIKSKEFD
                     AGTYNYVKTLSDIMAAALRTELMI"
     gene            6708..12143
                     /locus_tag="PKU88_07070"
     CDS             6708..12143
                     /locus_tag="PKU88_07070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPX69074.1"
                     /translation="MRFINTQYKVISISNEDRYGSTVVVEDTRRGRMLKILRIINYQK
                     ETQDFVDYMKLNMFDHRNLSHPNLANFYFFSRILEIDTKPVVANKYYYTYEHFNGVGL
                     FEFINNQNFDMLLDISVQLCSLVKYLHLRGFLFCSVNINDLFVVYEKGKALLKVSKLP
                     YVKGTEKSVMFDRESNYFISPEAFQFGDYSRASDIYLIGALMFYIFTGAKVEVSNFKD
                     ELKKFRNIEDKNKSQVIEIIKKCTSLKPGDRYESVEKIVEDINRRLNKNFNIINKKHI
                     QKLPRHITRLVSREHHFKRVIESAKGFLFENKLQPKAMIIKGSMGTGKSVFIKASGYR
                     LEQEGISVVYNVLKENVVSSFYYIILLIKEIMRVTDKEIIDRYIADISYIIPDISSVP
                     AAQARSSAIRQEDKIKLVYRLGNFLLEASAKHSFALAVSGFDYIDEDSLSVLNYIMRS
                     EGKGKIYLLLGAGIQSIEENDKLKEFCSSLAEAGYAECIELNNLNINETAEYIRLLLG
                     MDKPFLDFAAKVYKETEGNPKFIYEIIYFLVINNHIFVNDKGEWVLNRVDLNKLNLSF
                     NIDDFILNKISKLSPLQKEIITTVSIFNTAVSPDILKKMLDMSEDKLNEQLSQLDTIN
                     ILSKKVDDWGISFDFSSINLKKSIYERIPQDIKLKSHLIASYILEDKFNKEKRQNKDE
                     LIFHMAKAMRYKEAIEYLMNSAEKMISSNLYSQAIQFLMQSYNLFAPEDVSNDKKIVC
                     SKLGELYEQIGEYSNSMYFYDEAERSAVIVNDSKVLIDIFIKKFSLSYKIGDKKKAIE
                     YAVKAKRLLKRFSYPGGLYELVICFNDVMSNKRKYSSYIKVLKKLIDTIDTERHECYY
                     ARLLVTYGRTICNKNRYEEGLSELIKGMAILEKLSEYKYMPYVLNSLGYVYSEYYNDM
                     EKAREYYEKCLSVSQKLNDLSSMEKSYNNIAEIYRIEDRYSEAIEYYNKGLQVVNVSR
                     NMMIKALLNTNLIIASIGMEDLKKAALYIDISEELLRNLKDSGNVMHFFYQCKAEFLY
                     AMGEYERAAEYAQKSVNMCITWGITENYEAHFMKLLSEIQLSGKLNHGRIKEILDKVF
                     EVNWYKLGRTACVKLAEIYISKGMRKKGLELLARGLRYASNIDTDMLRLRYDYVEALA
                     KKGAERLEGLTKLAGLMESAENNEIKWKIYKAIAEELMAQKNYREALKYLITSLSYLR
                     KLVYNVPDEYKIRFINSHDRNSVKENLCRAAELMTGNEPGEKAAKEYMTGNISLQEMD
                     KYFDYTEYRDMYRYKEADETAAGEDLDTSTRVKFLGKVQELVSRFSDDETANLKHMVD
                     LFAEITQAKNAFIATLQEDGSLNVIVSYNRYQETPFYKYLIEQAKQKRDSIIVNDTFD
                     YNAKKGDILIPKDITAVFCIPIMIQKERDGIGIELVEERRRYKEQGDNPIIGYIYLDT
                     DSIISNFTQESSLFCMMAAKIAYVLTDNYNMKVMSTVDKLTRLYTRKHFESALSKELA
                     YAEKEGGQFSIIMADIDKFKSVNDRFGHQKGDEVLQNISSIIMSSVRKGDICGRYGGE
                     EIIILLPGTDAKGAYSVAEKIRKKIENAKLLGLNTPLTISLGISSYPEHGTWAKDLID
                     KADQALYHVKESGRNGSRIYELNMSNSIKRIDRLAGIISGNIVEDQRKTETMLEILDL
                     QRDSDKTLEEKLFSFLGRIIEVSEAQTGGIFCIEEAGNEKRISRKILRKRLADKEEEV
                     YYNEGIVQKCMETGKGEYQIDWNSYPGIDILTGMPDWQSVIAVPMIDRGRIKGVTYLS
                     VPIKSKEFDAGTYNYVKTLSDIMAAAF"
     gene            12270..15074
                     /locus_tag="PKU88_07075"
     CDS             12270..15074
                     /locus_tag="PKU88_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPX69075.1"
                     /translation="MKGFGRIKTKGITILLVIIVLAIAFFSNIAEYLINYQWFSKLGY
                     QEVFFKKLFTQMQFFIPALFVLFFLFYLYLKSINTHSVRHSGLVLSSVEKKIRTKISV
                     YGSLGLAFVFSLLFVFDIWYDFLIFINKTPFGLADPIFGKDIGYFVFSLPFFNKLYNF
                     LLMIIFAFAAITFLFNAYNFLTTKVPDEKLNIDIRPVGNSKDMYRNILRTASKQLMFL
                     GGLFFLVLAFGFYLRTFDLLYSSRGVAYGASYTDIKITLPAYYIYMGICILTAALLIL
                     NRNKKNIKLIVLGPLLLVVAMIAAGVIYAVVQNMIVAPNELAREEEFLQYNINYTNYA
                     YNLDKVTEKEFSVNQALTREDIEENEVTVNNIPINDYRPAKDIYNQIQGLKSYYQFND
                     MDIDRYMINGIYRQVFISARELQSANIPKQTEARGASWINKYLKYTHGYGVSMSPVNE
                     VTASGQPRLFIRDMPLISDIDIKVDVPQIYFGESTKDFVIVNSREKEFDYPATNINME
                     TEYSGTGGISLNILNRILFAVREGELNLLLSQDINSKSRILINREIIDRVKKIAPFLT
                     YDEDPYIVIYNGKLYWLIDAFTTSSRYPYSERVNSQTSTNYIRNSVKVVIDAYNGTTE
                     FYIADANDPIIKTYSKIFNTLFKPISEIPEGLRSHIRYPQTLFDIQADIYKKYHMKNA
                     REFYNKSDVWDVSTQIYGTGTNQANAELVESSYLIMRLPDSKKEEFLLIVPYTPQGKT
                     NMISWLAVKNDGENYGEIILYSFPSGKIVEGPMQIEGIVSQDTIIGPQLNLLATGGNS
                     QVVRGNMLTIPIEESILYVEPIYVKALNANALPEQKKVIVYFKNKVVMEDTLDEALDR
                     VFPVKEKPGEKTQDEPVPTPGGNIDTSAEKLIKEANDTFEQAMNAQRSGNWAEYGSKI
                     KALEELLKQLSELTNIQGNQ"
     gene            15125..16030
                     /gene="rnhC"
                     /locus_tag="PKU88_07080"
     CDS             15125..16030
                     /gene="rnhC"
                     /locus_tag="PKU88_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HPX69076.1"
                     /translation="MNGTKDKYQYYNHLKNILEENGIEISLYEDINYGLQFSIIISGK
                     KHLIRVYENKKNGTRCDLSQIKDEAAYKLIDGLLRTGSLKAEKAEEPQEADAGLIGTD
                     ESGKGDYFGPLVIAGVYADAGMKKKLMETGAADSKTLSDARIRKLAEEIAGICKYSVV
                     IIGNKRYNELYEKIENLNKLLAWGHARVIENLLEETDCSYALSDQFGSPELIKNALLE
                     KGKNINLEQRPRAEENVVVAAASILARNEYVKAMNKLAEKYGMEFPKGSSSMVTEAAK
                     EFVLLHGKEKLSEVAKLHFVITGKI"
     gene            16128..16679
                     /locus_tag="PKU88_07085"
     CDS             16128..16679
                     /locus_tag="PKU88_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPX69077.1"
                     /translation="MKKVYVYLAEGFEEIEAVTVVDTLRRAEIDARMISITGKKNVTG
                     AHGIVVSADEIFENADNMGQDMIVLPGGMPGTRYLGEHNGLRKIIMDFAEKGKPIAAI
                     CAAPSILGKLGLLDKKEAVCYPGFEETLKGAVIGKNIVSHGSNFITSKGPGTAIYFAL
                     YLVEILRDKKTAEELREGMIVQG"
     gene            complement(16739..17887)
                     /locus_tag="PKU88_07090"
     CDS             complement(16739..17887)
                     /locus_tag="PKU88_07090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPX69078.1"
                     /translation="MNKTASVVIVGGGISGCAIAYTLAKKGVKDIVVIERDYLSSGAT
                     GRCGAGVRMQWGTEMNCRLAKYSIEFFERANEELEYEYDIEFKQGGYLIVASTEKEVN
                     QFKKNIALQNSLGIPSRLLTPKEAKEIVPHMDETKIIAATFNPKDGHLNPFHTTQAYA
                     NAAVRLGVEIMKYTTVTGIKVENGKVVGVQTDKGYISTPVVVNAAGGYSQLIAKMVGI
                     DLPVYSERHQILVTEPVAPMQGPMFMSFSLNIYCQQTPHGSFIMGRGDANEPRDLRIT
                     SSWHFMEEMAKTCTELLPPLKELRVVRQWSGLYNISPDRQPIYGPVKEVEGFYLACGY
                     SGHGFMFGPVTGVVMAEAILGETPTIPVHMLDKDRFERGELLLEPSVV"
     gene            complement(17880..18644)
                     /locus_tag="PKU88_07095"
     CDS             complement(17880..18644)
                     /locus_tag="PKU88_07095"
                     /inference="COORDINATES: protein motif:HMM:NF037640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPX69079.1"
                     /translation="MLERTGVPTKENIESVTPLKERLSKGPVAIIECFQEIPCNPCYT
                     SCKKGAIKELIDINDRPILDPEKCNGCGVCISNCPGLAIFVVDESYSETEALVKIPYE
                     FVPLPKEGSFVTGLDREGKPVCRAKVEKVLNTKAQDRTPVVSLIVPKEFSMTVRFLSL
                     NDHYSDNTIVCRCEEITLGEIREYIRRGYQSLEEIKRISRTGMGPCQGRTCSQLIMQE
                     LAAATGKRLSEIKMSAFRPPAVPIKLGQLLGGDDHE"
     gene            complement(18644..19738)
                     /locus_tag="PKU88_07100"
     CDS             complement(18644..19738)
                     /locus_tag="PKU88_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPX69080.1"
                     /translation="MIHTEIAIVGGGPAGLCAAIKAAESGAKVIVLDRNSQLGGQLIK
                     QTHMFFGSQKQYASHRGIDIANILISKLEDYKDKITVMLDATVIGLYEDRVLTIDQGG
                     KYIKIKPDTLIIATGASEKFQAFPNNDLPGIYGAGAVQTLMNVYGVAPGESVFMVGAG
                     NIGLIVSYQLMQAGIKVKGVICRGPKFGGYAVHASKLRRLGVPVMPSHKVVYAHGKDK
                     LERITLIKLDENKKEIPGTEFDIEVDVLCIATGLTPLAELLWQMGCEMKYISELSGHV
                     PLRDKNLMTSLDGIYVAGDVAGIEEASSAMVEGSLAGLCAAKAAGHIHPDFEALVADC
                     HKQLYDLRSGPEGEAIRKGLSKAELKEVGL"
     gene            complement(19735..20040)
                     /locus_tag="PKU88_07105"
     CDS             complement(19735..20040)
                     /locus_tag="PKU88_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPX69081.1"
                     /translation="MRIEQHPIITFNKGRKVQFTFDGKVYEGYEGEPIAAALHDAGIR
                     VLGHTHKLGRPRGFYCAIGNCSSCLMVVNGKPNVRVCVEKLKEGMAVETQIGRGDIK"
     gene            complement(20227..21639)
                     /locus_tag="PKU88_07110"
     CDS             complement(20227..21639)
                     /locus_tag="PKU88_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX69082.1"
                     /translation="MPDNNEIKEFDIFDVEDGELLKIILDSINDAICVVDENCITRYW
                     NNAAEKLYNIGKDKILGRHIKEFFPNSLLPKIIKERKSYDNIYNNPREGCYNVISASP
                     LYMNGRLVGGISLDRDATEFIKTSELLMKAQSNLNVLEHEMSIINKNRYSFSQILGNN
                     RAFRDSIRLARDISRSNINVLITGESGTGKELFARAIHMESGREGYFVPINCSAIPHE
                     LIESELFGYTAGAFTGALKTGKIGKIELAHNGTLFLDEIGDMPLSMQPKILRVLENGE
                     MTRIGSEKTIKTNIRIITATNKNLSEMVEAGLFRKDLYYRLNSVVINLPPLRERKDDI
                     PLLVNRFIEDYCMEYGINILGITPEVLDSFVNHSWEGNIRELKNAVERIVVLARNSNT
                     GVIDSSFLPQSLGGIRKTISAVKQDSIYDLNTILENAEREALINVMRISGGNKAHGAK
                     LLNIPRSTLYFKLKKHNLEK"
     misc_binding    21780..21994
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            22052..23497
                     /gene="proS"
                     /locus_tag="PKU88_07115"
     CDS             22052..23497
                     /gene="proS"
                     /locus_tag="PKU88_07115"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPX69083.1"
                     /translation="MADKKQQEFVKEITPMEEDFSQWYTDVILKTDMVDYAPVKGCMV
                     IKPYGYGVWEGIQRVADRRFKATGHKNAYFPLLIPESYLKKEADHFEGFAPEVLWVTH
                     GGSEELTERLFIRPTSETIICDMYSRWVQSYRDLPLLINQWCNVVRWEKTTRPFLRTA
                     EFLWQEGHTVHADEEDAQRETLLILEIYRQVAEDELAMPVILGQKSEKEKFAGALRTY
                     TMEAMMHDGKALQAGTSHNLAQHFSKMFDIKFLDKDGVLKYAHSTSWGISTRLIGGII
                     MVHGDNRGLVMPPRVAPVQVVIIPIAFHKEGVLDKAKAVKDELEKCFRVEMDDRDTYS
                     PGWKFNEWEMKGVPIRMEIGPRDIENNQVTIARRDTLEKFTVSMDNLTETVSGLLDEI
                     HTTMFNKALKNMEEKTYTTTDYEEFKKIMQETPGFVKTMWCGSRECEDKLKEDTAATI
                     RCIPFRQEHVGDKCLICGGKAEKMVYAAKAY"
     gene            complement(23622..23834)
                     /locus_tag="PKU88_07120"
     CDS             complement(23622..23834)
                     /locus_tag="PKU88_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69084.1"
                     /translation="MAKLDMIGQIADLKEIDYKNTLAITSIIELLIEKGILDSSEVAA
                     KAQYLEDQTLSEIRRNSRFGIRTDFR"
     misc_binding    23979..24179
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24233..25507
                     /gene="serS"
                     /locus_tag="PKU88_07125"
     CDS             24233..25507
                     /gene="serS"
                     /locus_tag="PKU88_07125"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPX69085.1"
                     /translation="MLDIRAIRSNPEEVKRKLTRRGQEFRIDEVLKLDEQRRQVIYEV
                     EELKSRKNKVSEEVAKLKRAKQDAEAVVLEMRQVSEKIKEMDERVRNLDEEITGILLT
                     IPNTPYDGIPDGSSDADNVEIRKQGEPTKFEYEPKAHWDIGVDLNILDFETAGKVTGA
                     RFTFYRGLGAKLERALINFMLDTHTENGYTEIFPPFMVHRRSMTGTGQLPKFEEDAFK
                     VQNTEYFLVPTAEVPVTNMYREQILDGSMLPIRHTAYTACFRAEAGSAGRDTRGLIRQ
                     HQFNKVELVKFTNPENSYEELEKLTEDAERILKLLKLPYRVVCLCAGDLGFSSAKTYD
                     LEVWMPSYGRYVEISSCSNFEDFQARRADIKFRPEPKEKPRFVHTLNGSGLAVGRTVA
                     AILENFQQGDGSVIIPEVLRPYMGGLDKITKA"
     gene            25626..26915
                     /locus_tag="PKU88_07130"
     CDS             25626..26915
                     /locus_tag="PKU88_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX69086.1"
                     /translation="MSGKEVKKHKHSTLSGYIVQTLLMVTPLAGIAGTLMSIYIVRLE
                     GLQLKLSVACYLLAGIMLGIMASFRNYSKFIKPLNEISNLADTLKNSNITYRIDTSKA
                     GGQLEIIESLNESVSNLRSTVKDAMEVGSQVSAAPLEMKKSIQILSSISEQAAAALSE
                     LAKGAVEQAGSIEEISNSISGIVSGLSKITTDMDDTDKLAEKAIDTIKLGENSVNYQE
                     VKINENKEFVTSVSSAVSALAEKSKEIGDILLVINGIAEQTNMLSLNAAIEAARAGEQ
                     GKGFAVVANEVRKLAEQSGNSVQKIEELIREVQSGIEKAVQEMTKVESIADEQQKAMS
                     ATVKSFAGIFEAVSEISANIREVNSESVNIAKNARQAETAIGQIVKISEQAAAATEQV
                     SASTDEQANAVRSILESSEKLSELAGRLKGSINNFTV"
     gene            27054..27143
                     /locus_tag="PKU88_07135"
     tRNA            27054..27143
                     /locus_tag="PKU88_07135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27088..27090,aa:Ser,seq:tga)
     gene            27343..27435
                     /locus_tag="PKU88_07140"
     tRNA            27343..27435
                     /locus_tag="PKU88_07140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27379..27381,aa:Ser,seq:gct)
     gene            complement(27517..28713)
                     /locus_tag="PKU88_07145"
     CDS             complement(27517..28713)
                     /locus_tag="PKU88_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX69087.1"
                     /translation="MKGKGMMTRLTHAGENAIAKKTAASVSVPKVIPIYMSSVFSFDD
                     VDSLDAVYAGTAQGYVYSRMTNPGTDCVEEVLAQAEDCGGALVFSSGMAAIVTTIISQ
                     VKAGDHIISSPVLYGGVYDYLKNELPRFGVEVTFADFEKDDIESLIRSNTKILYTETI
                     SNPLMAVEDLPSISKIAHSHGCKLIVDNTFATPAIAQPLKLGADISVYSATKYLGGHS
                     DIIGGAVMSDAENIAHIHHFLTLYGAIMGPFDAWLLTRSLRTLDMRMERHSANALKVA
                     EFFEKHSKIERVYYPGLASSPYKKRTQESFTYGRCGGMLSADIAGGEKGACTFIRECK
                     NIKFVPSLAGVATSISYPAKTSHRAYSPQELKNVGISMGLLRFSVGLENIDDVLEEFD
                     AALKKV"
     gene            28892..29770
                     /locus_tag="PKU88_07150"
     CDS             28892..29770
                     /locus_tag="PKU88_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPX69088.1"
                     /translation="MKKLKFIYNPYSGEGVFKDRLDYLIERFQQAGYMMVPFRSTNIN
                     TLDRAFFDIHEDYEAVCISGGDGTLSTVTNIMASRKLKLPVGIFPFGTANDFATHLNI
                     PGDIEACCEIIEKGRTKKVDIGRVNNSYFLNVCSAGLLSDVAYETDTNLKNALGKLAY
                     YMKGIEKIPRFTPFKMRLQYGSNVIEDNFLLLLILNGRSAGGFSRLAPNAVIDDGLLD
                     VIAVKNTNITNILALFLKILRGEHIGDTNLYNFQADRLAITCEGGCETDIDGERGPDF
                     PLDIEVKKHFLKVFVP"
     gene            complement(29883..30740)
                     /locus_tag="PKU88_07155"
     CDS             complement(29883..30740)
                     /locus_tag="PKU88_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX69089.1"
                     /translation="MIEYRKLAHEDYDDILDICKDIWDGTDYLPEIFHKWVDDKGLFL
                     GAVDTDTNKVIGTDKYSVLYDGSGWLEGLRTHKDYRGRGIGKGLAVRTFNKALEDLGS
                     GRINKIAFSTSALSVESIGLMTKLGFRLKQEYKMVWKDYSDADNSLSIGDFAAESWKP
                     SYDEFANQPYIKRRDGILPFVFLFQKPTPELYEELLKEKCFVSINGHKGLFKLKGDPH
                     FIVFDESFEGINTFMNYYLLALAGKCPVPPLVSVMPADTELIEALNAAGFGMMDPSTC
                     DYLYYIYEG"
     gene            30937..32022
                     /locus_tag="PKU88_07160"
     CDS             30937..32022
                     /locus_tag="PKU88_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HPX69090.1"
                     /translation="MEAEKYKVAETAVEEADLSKLTEEQKQRLEEIIKTINLEDSQFI
                     LQYGVGAQSQIAGFADNIVSEVRAKDSGYVGDILTDLMLKVKELDVDGVSSGKGFMSR
                     FPIFGSLVNNTQKFAARYQKLSTEIEKIVDELDKSKMQLLKDITLLDYLFEKNLEYLG
                     NLNYYIMAGTLKLKEINEKIIPEMQSKVNETNDPLDAQKLNDLIQMANRFEKKIHDLK
                     LSRMVSLQTAPQIRLIQNNNQVLVEKIQSSILNTIPLWKNQIVIAISLAKQKNALAVQ
                     KEVTDTTNTLLQKNAEMLKTGSIDLARETERGVVDIETLKKVNEDLIATIDETLNIQK
                     EGRIKRKQAEEELAAIEKELKQKLIGK"
     gene            32053..33822
                     /locus_tag="PKU88_07165"
     CDS             32053..33822
                     /locus_tag="PKU88_07165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPX69091.1"
                     /translation="MNQKWSLDELYKSFESKEFKKDYEKCIKDIERIKEWAESNFKDS
                     SDAAHKVRRYIKTINEFYMLYSRLYSYAQLTVSVEARNEKALKVIDCLEEKATELTKP
                     EVSFQKWLGTISNINEIIASDSYLKKFEFFFRENLEKSKYLLSDKEEALISKMKTTGS
                     AAWEKLQELLTSTLLVEITLDGEDKKLPLPIIRNMAYNKDAELRKKAYEAELKAYQKI
                     DESVAACLNGIKGEVITVSRMKGYKSPLEKTILDSRMDLETLYAMIAAMRESLPDFHR
                     YFRKKAELMGYKNGLPFYEMFAPMGDADMQFSYKEARDYIVASFRTFSDKLADYAAHA
                     FDNRWIDAEPREGKRGGAFCENLHSIGESRILTNFDGSFSNMTTLAHELGHGYHGACL
                     MKEEYLNSDYPMPIAETASIFCETIVKEAALKTANKEEAFSMLENDISDSAQVIVDIY
                     SRFIFESEVFKRRESGSLSVNELKEIMLDAQKEAYGNGLDPDFLHPYMWLCKPHYYSA
                     DYNFYNFPYAFGLLFSKGLYAEYLKKGKEFTAEYDRMLSVTGKMSIADVAKVMNIDVH
                     DPGFWRNSLELIEKDIDRFIKLG"
BASE COUNT        10847 a         6324 c         7860 g         8791 t
ORIGIN      
        1 tgtaagcaca gcaatgtgtt gagctgaccg atactaatag gtcgagggct tgatctagta
       61 agcattttac gccaacaaat tctctttgat acgatcctcg tgagaattca cgcggaatgg
      121 agcaaagcat tagaatttgt cagcggaaaa tgcgttgctt aaacaaactt atgaattatt
      181 acggatattg tgcagttttg aaggtacaac accggttgtt ggtatcgctc ttagcggtac
      241 gcgcaatttg gaagttacct tttaaaatcc aatatggatt gataaaatta atccaaccga
      301 gaagcatcag aagcgagcag ttgtccggat gcttaagaaa tgaatagaag agtatcgtca
      361 ggaccaagca ggacaaggaa gatgagtttg agtgcgcgga atgtatagag acatacatga
      421 gcacacgaaa acgaaatctg acgcagtaat gcgcaggtca tcacgatacg aaagatctgg
      481 tggcaatgac ggagaggcca cacctgttcc catcccgaac acagaagtta agctctccgg
      541 tgtcgatggt actcggaccg cagggtcccg ggagagtaga tcgccgccag gtaactaaga
      601 acgatagaag aaattccatc gttctttttt atacacttgc cttgtatggg aaatgtattc
      661 ttcctgttac ctgttggcaa tgaaacggcg gttcgtttat tgcatttata tgcaagttgc
      721 taaattttct ccaaatttct ctaaatttat ggtaaaatta agaaacagca gttgcatttt
      781 tataacaaat tttcaatgag gtttttgcat acctgcaaaa atctatccga gggttaaaac
      841 gaggcggaag acatgcttac cgccttttag tcaaagaaag cagcacttac cattgtaaaa
      901 gtgaaggaca tcttcttgcc tcgttataat ttttcgaatt gatttgaaga aaggaggatt
      961 tcagatgcag ttcattaacg agcagtataa ggttgtcaag gaaaagagtt ccgatgcata
     1021 cggtacaacc tatatagtag aagatattca aaaggatagc cttcttaagc atttgagaat
     1081 tataaacctc caaaatgaga ccagggactt catagactat atgaaaaaca atttttataa
     1141 ttattccaaa tattatcatc catatctgat agattttcat tttttcaaca gaatcagact
     1201 tattgattat aaacatactg ttttaaacca ctattattat acctatgatt actttgaagg
     1261 ggttaattta tttgattact gtaaagggaa agacttcgat tcgattctgg acctggcggc
     1321 agaactgtgt gaggctgtta aattcttgca cttgagaggc ttcttgcttt gcagtgttga
     1381 cagtaatgat tttcaaatag tccgcgacgg agaaaaaggc cacttgaaga tattggcatt
     1441 gccatatcct cggggaaccg gaagcaggat aataataaac aaaaaaagta catgttttgc
     1501 agctccggag ttaaataagg atgagagcta ttcagttctg acagatgttt atattattgg
     1561 ctcggtcatt tcttgtatgc tcaaagcatt caaccattct gaggaaaaat ctgaggctaa
     1621 ccacagggct attatggata taataaaaaa atgcatggct gagaaacctg aagacaggtt
     1681 tcaaaccata gatcaaatta ttgcaagtat aaacaattac ttcggaaaag agtataggac
     1741 aataaataaa cagtacattc aggcaatgcc ccaatacaga ataaatccat cgtcaaggca
     1801 ctatcttatt gacaagatat taaacaatgc gaaagagcat ttttttatca atcggcccaa
     1861 taaggtttct ttggtggtgg ggctcgaagg cacaggaaaa gataattttt tggaaatttt
     1921 gagcattaag gcgcagcacg agggtttcat atccgtcaaa acggttctca atgaatcaga
     1981 ggtattaaga ttcagcgttg ccgaaacctt tgtgagagga ataatcaaat atgcagataa
     2041 agagattatt gataagtatg ccgaggcagt aaataatgta atgcttacga tctccaaatt
     2101 caaatcaata tctccaaagg tagaggatga atatttcgag aagggcagta aggaaaagtt
     2161 tatacaaaaa ctaagcaatt ttatagagga agccacaaag aagtcacatt gtatttttat
     2221 tattgaaaac tttcagtgga tagatgagga ttcattgagt ctgattgatg gaatgttaaa
     2281 gagccgaaat agttcaagcg cttatattgt acttgccaca gacaaggaga cctattccaa
     2341 aaacatcaaa ttaaaggaat actgcaggaa gcttaagaaa gcgaacttaa tgaataatac
     2401 tattgtgctt aagaacttca actttgagga gacagccgga tttataaagc ttattctgag
     2461 cttggacaaa gtgccccatg aattcgcaaa aattatttat gacaggacaa aaggaagtcc
     2521 ggattatata tatgatacta tatatatgct tttttcaaac aacaacatat atattgacga
     2581 caaaggctgc tgggtgcttg ataatgtaaa ctatgaattg ctgaatgttt cctatgccga
     2641 ggatatggat atgctgaaca gtatttacaa gcttgactcc aattatcagg atattcttaa
     2701 ggcggtatcc atttttgata gtgcggtttc agcagatatt gcagaaggtt ttgtcgaagt
     2761 taaaggtgaa agattagtca gccagatgaa ttacctttct tatataagca tttttgtaag
     2821 aaaacagaat gactggggcg tatcctacag cttcagttca ttaaaattga aagaatcgat
     2881 atataaaagc ataccaaagg aattgagacg aaaatatcat gaaaaagctt cttatatact
     2941 gaaaagcaag ctcagcgaca aaaacataaa aggtgaagat gagctgatgc gccaaatgct
     3001 aagagccaat tggcatcttg aagtaaagga atatatttca gactctgtca ggaagatgat
     3061 ggaaaacaac tcaatatatc agacaataca attcctcgag catgttaatg aattgctgtc
     3121 caaggagaat gtaacagaag agagaatatc ggtttgcagt atattgggag agttatatga
     3181 acgtataggt gagtattcaa aagctgttta ttgctttaac attttggagg atatagccga
     3241 gaggaaagga aataattgct tgcttgcaga cgtatatata aataaatact cactactgta
     3301 caaattgggt gataggaagg tttcattaaa gtatcttgct tatgccaaga accttttgag
     3361 aaccattgat tataaaaagg gcatgtacga acatataatt gtaatgcaca gaatgatgtt
     3421 tcataaaagg aaattcagca gttacataag gatattggaa agtgccttaa aagaaatcga
     3481 taaagaggaa tacaagttca tatatgcaag gatgcttggc atttatggca ggctcaaggc
     3541 ttataagggc gaatatgacg aaggccttat catgttggaa aaaagtattc agattcttga
     3601 aagcatcggt aattatccaa agatgttgta tccgttgaat tcaattggaa caatatatta
     3661 taaccattac accgatatac aaaaggccag ggagtattat gaaagatgtt taagcataag
     3721 tcagagagta gggaatgtgt attatgagag tataagctat aatagtatcg ccgatcagta
     3781 tagaatggaa gacaagtatt ccacagcact gcaattctat cagaattccc ttaagaaagc
     3841 taagcagata agggataagt atacggaatt cataataaat cttaatatgt ccctgacaaa
     3901 cattgaaata gaggactaca aaaaggttct tcttaccctt gacgatatgg aagaagagat
     3961 agcaaactcc agatattcgg gagaccttat ggactatttc tatcaatgca aggcggaatt
     4021 cctttatgcc atgggagaat acgaaagagc agcggaatat gcacagaaat ccgtgaatat
     4081 gtgcataacc tggggtatta cggagaatta cgaagcacat tttatgaagc tgctgtcgga
     4141 gatacagctg agcggcaagc tgaaccacgg aagaatcaag gaaattttgg ataaagtatt
     4201 tgaagtgaat tggtacaagc tgggacgcac agcctgtgta aagcttgcgg aaatatacat
     4261 ctcaaagggt atgaggaaga aagggctgga gcttctggcc agaggactca ggtatgcatc
     4321 aaatattgac actgatatgc tgcggctgag atacgattat gtggaagctc ttgcaaagaa
     4381 aggggctgag aggctggaag gccttacaaa gctggcaggg ctcatggaat cggcggagaa
     4441 taacgaaata aagtggaaga tatacaaggc aatagctgaa gagctgatgg cgcagaagaa
     4501 ctacagagaa gcattgaaat accttatcac atccttaagc tatttgagga agctggtata
     4561 taatgtgcct gacgaataca agataaggtt cataaactcc catgacagaa attcggtaaa
     4621 agaaaacctg tgcagggcag cggagcttat gaccgggaat gaaccgggag aaaaagctgc
     4681 caaggagtat atgacgggaa atataagcct gcaggaaatg gacaaatact ttgattatac
     4741 agaatacagg gatatgtaca gatacaagga agcggacgaa acagcggcag gggaggatct
     4801 ggatacaagt acccgggtaa aattcctcgg aaaggtgcag gagctggtaa gcaggttcag
     4861 tgatgatgaa actgccaacc ttaagcatat ggtagatctg tttgcagaga taacacaggc
     4921 aaaaaatgca ttcatagcga cgctgcagga ggacggcagc ctgaatgtaa tagtatccta
     4981 caacaggtat caggaaaccc ctttttacaa gtatttgata gaacaggcaa agcagaaacg
     5041 ggacagcata atagtaaatg acacctttga ttacaatgcc aaaaaaggag atatattgat
     5101 accaaaggat ataacagcag tgttctgcat accgataatg atacaaaaag aaagagacgg
     5161 tataggaata gaacttgtgg aagagcggag aaggtataaa gagcaagggg acaacccgat
     5221 aataggctac atatacctgg acaccgacag cataataagc aacttcaccc aggaaagcag
     5281 ccttttctgc atgatggcgg caaagatagc ctatgtactg acagacaatt acaacatgaa
     5341 ggtaatgtca acggtagaca agctgacaag gctgtacacc aggaagcact ttgagtcagc
     5401 gctgtcaaag gagcttgcgt atgcggaaaa ggaaggggga cagttctcca taataatggc
     5461 ggatatagat aaattcaaga gtgtaaacga ccgcttcggc catcaaaaag gagacgaagt
     5521 gctgcagaac ataagcagca taataatgag cagcgtaaga aaaggtgata tatgcggaag
     5581 atacggagga gaagagataa taatactgct tccggggaca gatgccaaag gagcttactc
     5641 cgttgcggag aagataagaa agaagataga gaatgcaaag cttctggggc ttaacacgcc
     5701 tcttaccata agcctgggga tatcctcgta tccggaacac ggcacatggg caaaagatct
     5761 gatagacaag gcagaccagg cgctgtacca tgtaaaagag tccgggagaa acgggagcag
     5821 gatatatgag cttaacatgt caaacagcat aaagagaata gacaggcttg cggggataat
     5881 atcgggaaac attgtggagg atcagaggaa aacagagaca atgcttgaga tactggatct
     5941 gcagagagac agtgataaga cactggaaga aaagcttttc agcttcctgg gaaggataat
     6001 agaagtatcg gaggcacaga caggagggat attctgtata gaagaggcgg ggaatgaaaa
     6061 gaggataagc aggaagatat tgaggaagag actggcggat aaggaagagg aagtttacta
     6121 caacgagggg atagtgcaga aatgcatgga aaccgggaag ggagaatacc agatagactg
     6181 gaacagttac cccgggatag atatacttac ggggatgcct gactggcaat cggtgatagc
     6241 agtaccgatg atagacaggg gaaggataaa gggagtgacg tatctttcgg tgccgataaa
     6301 gagcaaggag tttgatgcgg gaacctacaa ctatgtcaag acactgtccg atattatggc
     6361 cgccgcatta agaacggaat taatgatata accatacagc atgtacatca aagagaaaat
     6421 ttggtatatg ctgttatttt tttttatagt atgatagaat attgtaataa ggcaaaaaaa
     6481 tgtcctccaa caagtgtgag catatttgcc gccttattga aacgcgcaga ggtaagaaaa
     6541 tttttctgca atcgaaaaat tttctgctga tgcgttataa atacatttca tttaacagat
     6601 tgtactacaa attgtattaa tatttccatt caattgattt tgctataata aatctttttc
     6661 acgatatata ataactgtac aagttattag tgcttggggg aattaaattg cggtttatta
     6721 atacccagta caaagttata tctatcagta atgaggatcg ctacgggagt actgtagttg
     6781 ttgaagatac cagaaggggc cgcatgctca aaatcttgag gataatcaat tatcagaaag
     6841 aaactcagga ttttgtcgat tatatgaagc tcaacatgtt tgaccacagg aatttgagcc
     6901 atcctaatct ggcaaatttc tattttttca gcagaatatt ggaaattgat acaaagccgg
     6961 tagttgccaa taaatattat tatacctacg aacactttaa tggggtaggt ctttttgaat
     7021 ttatcaataa tcaaaatttt gatatgctgc tggatatttc ggtacaactg tgctctctgg
     7081 taaaatatct gcacctgagg ggatttctgt tttgcagtgt taacataaat gatctttttg
     7141 ttgtatatga gaaagggaag gctcttttaa aggtttcaaa gctgccatac gttaagggta
     7201 ctgagaaatc cgtgatgttt gacagagaaa gcaactattt tatatctccg gaagcatttc
     7261 aattcggaga ttactcaaga gcttccgata tttatcttat tggagcactg atgttttata
     7321 tttttaccgg ggcaaaggtc gaggtttcca atttcaagga tgagcttaaa aaattcagga
     7381 atattgagga taagaacaag tcgcaggtaa tagagataat aaaaaagtgt acttccttaa
     7441 agccgggtga caggtacgaa tcggtggaaa aaatagttga ggatataaac agaaggttaa
     7501 acaagaattt caatataata aacaagaagc atattcaaaa gctgccgagg catattacaa
     7561 ggcttgtatc aagagagcat cattttaaaa gagtaataga atctgcaaaa gggtttcttt
     7621 ttgagaataa acttcaacca aaggctatga taataaaagg atctatggga acaggcaaat
     7681 cggtttttat caaagcatcc ggttataggc ttgagcagga aggtatttca gtagtataca
     7741 atgtcttaaa agaaaatgtt gtcagtagtt tttattacat aattttgctg attaaagaga
     7801 ttatgagggt cactgataaa gagattattg acaggtatat agcggatatt tcttatatta
     7861 ttccggatat aagcagcgtt cccgctgctc aggcacgcag cagcgcaatc aggcaggaag
     7921 acaaaataaa gctggtatac agattgggaa actttctttt ggaagcttct gcgaagcatt
     7981 cctttgcttt agctgtaagc ggctttgatt atattgatga ggactccctc tctgtcttga
     8041 attacataat gagaagcgaa ggcaaaggca agatatacct tctgctggga gcaggaatac
     8101 aaagtattga agaaaatgac aagctgaagg agttttgtag ttcactggca gaagcaggat
     8161 atgcagaatg cattgagctt aataatctca atataaatga gacagcagaa tatataaggc
     8221 ttttgctcgg catggacaag ccgtttcttg attttgccgc caaggtatat aaagaaacag
     8281 agggcaatcc aaagttcata tacgagatta tatatttcct ggttataaac aaccatattt
     8341 ttgttaatga caaaggggaa tgggttctga acagagttga tctgaacaaa ttgaacctgt
     8401 cctttaatat agatgatttt atcctgaata agataagcaa actaagtccc ttgcaaaaag
     8461 aaatcataac gactgtttcc atattcaata ctgccgtgtc tccggatata ttgaaaaaaa
     8521 tgctggatat gagcgaggat aaactcaacg aacagctaag tcagttggat actatcaata
     8581 tactgtcaaa aaaggtggat gactggggaa tatcctttga cttcagctcc attaacctta
     8641 agaaatcaat atatgaaaga ataccccaag atataaagct aaaatcccat ttaatagctt
     8701 catatatatt ggaggataaa ttcaataaag aaaaaaggca aaacaaggac gaactgattt
     8761 ttcacatggc aaaggctatg aggtataagg aagccatcga atatcttatg aactctgccg
     8821 agaagatgat atccagcaac ttgtacagcc aggctattca atttcttatg cagtcatata
     8881 atttgtttgc cccggaagat gtaagcaatg ataagaaaat tgtctgcagc aagctgggtg
     8941 aattgtacga gcagataggg gaatacagca attccatgta tttctatgat gaggcagaga
     9001 gatccgcggt catagtgaat gattcgaaag tgctgataga catatttatc aagaagtttt
     9061 cactttctta caaaataggt gacaagaaaa aagcgattga atatgcagta aaggcaaaaa
     9121 ggctgctgaa gcgcttttca tatcctggag ggctctatga gcttgttatt tgttttaatg
     9181 acgtaatgtc aaacaaacgc aagtacagtt catatataaa agtattgaaa aagttaatag
     9241 atacaatcga taccgagcgg catgaatgct attacgcaag attgctggtg acttacggca
     9301 gaacaatatg caataagaac agatacgaag agggcctttc cgaattgata aagggtatgg
     9361 ccatactgga aaagctttct gaatataaat atatgcctta tgtattgaat tctttaggct
     9421 atgtgtattc tgaatactac aacgacatgg aaaaggccag agagtattat gaaaaatgcc
     9481 ttagtgtgag tcagaagctg aatgatctaa gctcaatgga aaaaagctac aataacattg
     9541 cggagattta cagaatagag gatcgctaca gcgaggctat tgaatattat aataaaggat
     9601 tgcaggttgt aaatgtttcc cggaatatga tgataaaagc actgctcaac acaaacctga
     9661 taattgcaag catagggatg gaggatttaa agaaagcggc tctgtacatc gatatctcgg
     9721 aagaacttct cagaaacctg aaggattcag gtaacgttat gcattttttc tatcaatgca
     9781 aggcggaatt cctttatgcc atgggagaat acgaaagagc agcggaatat gcacagaaat
     9841 ccgtgaatat gtgcataacc tggggtatta cggagaatta cgaagcacat tttatgaagc
     9901 tgctgtcgga gatacagctg agcggcaagc tgaaccacgg aagaatcaag gaaattttgg
     9961 ataaagtatt tgaagtgaat tggtacaagc tgggacgcac agcctgtgta aagcttgcgg
    10021 aaatatacat ctcaaagggt atgaggaaga aagggctgga gcttctggcc agaggactca
    10081 ggtatgcatc aaatattgac actgatatgc tgcggctgag atacgattat gtggaagctc
    10141 ttgcaaagaa aggggctgag aggctggaag gccttacaaa gctggcaggg ctcatggaat
    10201 cggcggagaa taacgaaata aagtggaaga tatacaaggc aatagctgaa gagctgatgg
    10261 cgcagaagaa ctacagagaa gcattgaaat accttatcac atccttaagc tatttgagga
    10321 agctggtata taatgtgcct gacgaataca agataaggtt cataaactcc catgacagaa
    10381 attcggtaaa agaaaacctg tgcagggcag cggagcttat gaccgggaat gaaccgggag
    10441 aaaaagctgc caaggagtat atgacgggaa atataagcct gcaggaaatg gacaaatact
    10501 ttgattatac agaatacagg gatatgtaca gatacaagga agcggacgaa acagcggcag
    10561 gggaggatct ggatacaagt acccgggtaa aattcctcgg aaaggtgcag gagctggtaa
    10621 gcaggttcag tgatgatgaa actgccaacc ttaagcatat ggtagatctg tttgcagaga
    10681 taacacaggc aaaaaatgca ttcatagcga cgctgcagga ggacggcagc ctgaatgtaa
    10741 tagtatccta caacaggtat caggaaaccc ctttttacaa gtatttgata gaacaggcaa
    10801 agcagaaacg ggacagcata atagtaaatg acacctttga ttacaatgcc aaaaaaggag
    10861 atatattgat accaaaggat ataacagcag tgttctgcat accgataatg atacaaaaag
    10921 aaagagacgg tataggaata gaacttgtgg aagagcggag aaggtataaa gagcaagggg
    10981 acaacccgat aataggctac atatacctgg acaccgacag cataataagc aacttcaccc
    11041 aggaaagcag ccttttctgc atgatggcgg caaagatagc ctatgtactg acagacaatt
    11101 acaacatgaa ggtaatgtca acggtagaca agctgacaag gctgtacacc aggaagcact
    11161 ttgagtcagc gctgtcaaag gagcttgcgt atgcggaaaa ggaaggggga cagttctcca
    11221 taataatggc ggatatagat aaattcaaga gtgtaaacga ccgcttcggc catcaaaaag
    11281 gagacgaagt gctgcagaac ataagcagca taataatgag cagcgtaaga aaaggtgata
    11341 tatgcggaag atacggagga gaagagataa taatactgct tccggggaca gatgccaaag
    11401 gagcttactc cgttgcggag aagataagaa agaagataga gaatgcaaag cttctggggc
    11461 ttaacacgcc tcttaccata agcctgggga tatcctcgta tccggaacac ggcacatggg
    11521 caaaagatct gatagacaag gcagaccagg cgctgtacca tgtaaaagag tccgggagaa
    11581 acgggagcag gatatatgag cttaacatgt caaacagcat aaagagaata gacaggcttg
    11641 cggggataat atcgggaaac attgtggagg atcagaggaa aacagagaca atgcttgaga
    11701 tactggatct gcagagagac agtgataaga cactggaaga aaagcttttc agcttcctgg
    11761 gaaggataat agaagtatcg gaggcacaga caggagggat attctgtata gaagaggcgg
    11821 ggaatgaaaa gaggataagc aggaagatat tgaggaagag actggcggat aaggaagagg
    11881 aagtttacta caacgagggg atagtgcaga aatgcatgga aaccgggaag ggagaatacc
    11941 agatagactg gaacagttac cccgggatag atatacttac ggggatgcct gactggcaat
    12001 cggtgatagc agtaccgatg atagacaggg gaaggataaa gggagtgacg tatctttcgg
    12061 tgccgataaa gagcaaggag tttgatgcgg gaacctacaa ctatgtcaag acactgtccg
    12121 atattatggc cgccgcattc tagatggacg ccatgtatgg cgtcccacat agggtcgcca
    12181 tacatggcga cctttattgc taaaacagtt tgaatattcc taatgtaaaa tgtataattt
    12241 atatatatgc aaattgggga gaggttatat tgaaaggatt tggcagaatt aaaaccaaag
    12301 gcattacgat attgctggta attattgtgc tggctattgc gttttttagc aatatagccg
    12361 aataccttat taactaccag tggttttcga agcttggcta tcaagaggta ttctttaaaa
    12421 agctttttac acagatgcag ttttttattc cggccctttt tgtattattt tttctgtttt
    12481 acctgtacct gaaatccatt aatacgcatt ctgtaaggca cagcggattg gtactgtcaa
    12541 gtgttgaaaa aaaaataaga acaaagatat ccgtttatgg ttctttaggc ttggcctttg
    12601 ttttttcttt gttattcgtg tttgatatat ggtatgattt tctgatattt atcaacaaga
    12661 ctccctttgg cctggcagat cctatatttg gaaaggatat aggatatttt gtattttcac
    12721 tgcctttctt caataaattg tataattttc tgctgatgat tatatttgca tttgcggcaa
    12781 ttacctttct ttttaatgct tataacttcc ttaccacaaa ggtgcccgac gagaagttaa
    12841 atatagatat aaggcctgtc ggcaattcta aggatatgta cagaaacata cttaggactg
    12901 cctcaaaaca gcttatgttt cttgggggac tcttttttct ggttttagct ttcggtttct
    12961 atctcaggac ttttgacctt ctttattctt caagaggagt agcatatggg gccagttata
    13021 ctgatatcaa aattactctt cccgcctatt acatatatat gggcatatgc atattgacag
    13081 cggcattatt gatattaaac agaaataaga aaaacataaa gctgattgtt ctcgggccgc
    13141 tgcttcttgt agttgccatg attgctgccg gagtgatata tgccgtagtt cagaatatga
    13201 tagtagcacc aaatgaattg gcaagagaag aagaattcct gcagtacaat attaattata
    13261 caaattatgc atacaatctt gataaagtta cagagaagga gttttcggtc aatcaggccc
    13321 ttacacggga agatattgaa gaaaatgagg ttacagtaaa taatattccc ataaatgatt
    13381 acaggcctgc aaaggatatt tacaatcaga ttcagggctt gaaaagctat tatcaattca
    13441 atgacatgga tattgacaga tatatgataa acggtatata caggcaggta ttcatatcag
    13501 ccagggaact tcagtctgca aatataccga agcaaacaga ggccagaggg gcgtcatgga
    13561 taaacaagta tcttaaatat actcatggat acggagtttc aatgtctccg gtaaatgaag
    13621 tcaccgcttc gggtcagccc agattgttca taagagacat gcctttgata tcagatattg
    13681 acataaaagt tgatgttccc cagatatact ttggagagag cacaaaggac tttgtaattg
    13741 tcaactccag agagaaggaa tttgattatc cggccaccaa tattaatatg gagactgaat
    13801 acagcgggac aggaggtata agccttaata tattgaacag gatacttttt gcggtacggg
    13861 agggagaact caatttatta ttatcccagg atattaactc taagagcaga atactcataa
    13921 acagggagat aattgacaga gtgaagaaaa ttgctccgtt cctcacttat gatgaagatc
    13981 cttatatagt catttataat gggaaattgt actggctgat agatgcattt acaacaagca
    14041 gcagatatcc atattcagag agagtcaaca gccagacatc tacaaactat atacgaaact
    14101 ccgtcaaggt tgtaatagat gcttacaacg gcactaccga attctatatt gcagatgcaa
    14161 atgaccccat aattaagaca tattccaaga tatttaacac gttatttaaa ccgatttctg
    14221 aaatacctga gggcttaagg tctcatataa gataccctca gactttgttt gacatacagg
    14281 cggatatata caaaaaatat catatgaaga atgcccggga attctacaat aaaagtgatg
    14341 tgtgggatgt gtcgacccaa atctacggaa ccggcaccaa tcaggcaaat gcagagttgg
    14401 tggagtcctc atatcttata atgaggcttc cggacagcaa aaaggaagaa tttttgctga
    14461 tagttccata tacccctcaa gggaagacca atatgatatc ctggcttgcg gtaaagaacg
    14521 acggggagaa ttatggggaa ataatactgt acagcttccc gtcagggaaa atagtagaag
    14581 gccctatgca gatagagggc atagtcagtc aggacaccat aataggcccg cagctgaatt
    14641 tgcttgcaac gggaggaaac tcacaggtag taagaggaaa tatgcttaca atacccattg
    14701 aagaatcgat actgtatgta gaacctattt atgtaaaagc attaaatgcc aatgcactcc
    14761 cggagcagaa gaaagtaata gtatatttta aaaataaggt tgtaatggaa gatacattgg
    14821 atgaagcctt ggacagggtg ttccccgtaa aagaaaaacc gggagaaaaa acgcaggatg
    14881 aacctgtacc aactccgggg ggaaatatcg atacttcggc ggaaaagctg ataaaggaag
    14941 caaatgatac atttgaacag gcaatgaatg cacaacgatc cggcaattgg gcggagtacg
    15001 gctccaagat aaaagccctg gaagaactgt taaaacagct tagtgaactg acaaatatac
    15061 agggcaatca gtgaataaat cggctttaag gctgcgccta taaacatttt ttggagtgag
    15121 gcaattgaac ggaacaaagg ataagtacca gtattataat catttgaaga atatacttga
    15181 agaaaatgga atcgaaataa gtttatatga agatattaat tatgggcttc agttcagtat
    15241 aattatcagc ggaaagaagc atcttataag agtttacgaa aataagaaaa acggaacaag
    15301 gtgtgactta tcgcaaataa aagatgaagc ggcctataag ctgattgatg ggcttttgag
    15361 aacaggaagc ctgaaggcag aaaaggcaga agagccgcaa gaggctgatg ccgggcttat
    15421 cggcaccgat gaatcaggta agggcgatta tttcgggcct ttggtgattg cgggagtata
    15481 tgcagatgca ggaatgaaga aaaagcttat ggaaacagga gccgcagaca gcaaaactct
    15541 ttctgatgcc aggatccgta agctggctga ggagatagcc ggaatatgca agtattctgt
    15601 ggttatcatc ggcaacaaga ggtataacga attatacgaa aagattgaaa atctgaacaa
    15661 gcttcttgcg tggggacatg cgcgggtaat cgaaaacctg ctggaagaaa cagactgcag
    15721 ttacgcactg tctgaccagt tcggcagccc tgagcttata aagaacgcac tgctggagaa
    15781 aggaaagaat ataaacctgg agcaaaggcc gagagcggaa gaaaatgtag tggtggccgc
    15841 tgccagtatt cttgcaagaa atgaatatgt gaaagcaatg aataagctgg cggagaaata
    15901 cgggatggaa tttcccaagg gaagctcttc aatggtgaca gaggctgcaa aggaatttgt
    15961 actgcttcac gggaaagaga agctgtcaga ggttgccaag ctgcatttcg ttataaccgg
    16021 aaagatatag tttccgggta ttcctaaaga gtatggttac gtgcttcgag ttacgggttt
    16081 cgcgattatg gcagggctat gaagaatttt gaaaggagga ttacggaatg aaaaaagtat
    16141 atgtctattt ggcggaaggc tttgaggaaa ttgaagccgt tactgtggtc gatacattaa
    16201 ggcgggcgga aatagatgca agaatgatat caataacggg aaaaaagaat gttacagggg
    16261 cccatgggat tgttgtctcc gccgatgaga ttttcgaaaa tgccgataat atggggcagg
    16321 atatgatagt tctgccggga gggatgcccg gtacaagata cttgggggaa cataacggcc
    16381 tgagaaagat cataatggat tttgctgaaa agggcaaacc catagcggca atatgcgcag
    16441 ctcccagcat tctggggaaa ctggggcttc tggacaagaa ggaagccgta tgctatcccg
    16501 gttttgagga aacccttaaa ggagcagtaa taggcaaaaa tatagtctca catggaagta
    16561 atttcataac ttcaaagggg ccgggtactg ctatctattt tgccttgtac ctggtggaaa
    16621 tcctgagaga taagaaaacg gcggaggaac ttagagaagg aatgattgta caaggataaa
    16681 agtaaaattc cggatgggcc ttataatcca tccggaattt ttttgcttta attgattctt
    16741 atacaacgga gggttcaagc aaaagctctc cgcgttcgaa tctgtcctta tccagcatat
    16801 gaaccggaat tgtaggagtt tctccaagta tggcttcagc cataacaact cccgttacag
    16861 gcccgaacat aaagccatgt ccgctgtaac cgcatgcaag atagaagcct tccacctctt
    16921 taaccggtcc gtatatcggt tgacggtccg gagaaatatt atacaaaccg gaccattgac
    16981 gtactactct taattctttt aaaggaggca gcaattcagt acaggtcttg gccatttcct
    17041 ccatgaagtg ccaactggat gtgattctca aatccctggg ctcatttgca tcgcctctgc
    17101 ccattatgaa gctgccgtga ggtgtctgct ggcagtatat attaagggag aaggacataa
    17161 acatcggccc ctgcatagga gctacaggct cagttacaag aatctgatgc ctttctgaat
    17221 ataccggcag atcaataccc accattttag caataagctg cgaatacccg ccggcggcat
    17281 ttacgacaac cggagtagaa atataacctt tatcggtctg tacgccaact acttttccgt
    17341 tttcaacctt aattccggta acggttgtat acttcattat ctcaacaccc agcctgactg
    17401 ctgcattggc ataagcctga gtagtatgga agggattaag gtgcccgtct tttgggttaa
    17461 atgtggctgc aataatcttt gtttcatcca tatgagggac tatttcctta gcttccttgg
    17521 gagtcagcaa tcttgaaggg atgcccaggc tgttttgcaa agctatattt ttcttgaact
    17581 ggttgacttc tttttccgta gaagcaacta tgagatatcc tccctgtttg aactcaatat
    17641 catattcata ttccagctct tcatttgctc tttcaaagaa ttctatactg tactttgcca
    17701 gcctgcagtt catttcagta ccccactgca tacggacacc ggctccgcac cttcccgtag
    17761 caccgctgct aagataatcc ctttcaatta ctacaatatc ctttacgcct tttttggcca
    17821 gggtgtaagc tattgcacag ccggaaatac ctccgcccac tattactaca cttgcagttt
    17881 tattcatgat catcacctcc aagcagctgt cctaatttaa tcggcactgc cggcggcctg
    17941 aatgcggaca tctttatctc agagagccgt ttcccggtag ctgcggccag ctcctgcata
    18001 atcagctgac tgcaggttct tccctgacag ggacccattc cggtcctgga tattctcttg
    18061 atttcctcga ggctttgata tccgcgcctg atatattctc ttatttcacc cagagtaatt
    18121 tcttcacatc ggcagacaat agtattatca ctgtaatggt cattaaggct taaaaacctt
    18181 acagtcatgg aaaattcttt tgggactatt aatgatacta caggagttct gtcctgtgct
    18241 tttgtattca gaaccttttc aacctttgcc ctgcatacgg gctttccttc tctgtccaaa
    18301 cctgtgacga agcttccttc cttaggcaat ggcacaaatt cataaggtat ctttaccaga
    18361 gcttcagttt ctgaataaga ttcgtcaact acgaaaatcg caagccccgg gcaattgctt
    18421 atacaaacgc cgcagccgtt gcatttttca ggatctaata taggacggtc attaatatct
    18481 atcagttctt ttatcgcacc ttttttacag gaagtgtagc agggattgca gggtatttcc
    18541 tgaaagcact ctataattgc taccggacct tttgacaacc tttccttcaa aggtgttaca
    18601 ctttcgatat tttcttttgt aggcacaccg gttctttcaa gcattataaa ccaacctcct
    18661 taagctcagc tttggataga cctttacgga ttgcttcacc ttcagggcct gaccgtaaat
    18721 cataaagctg tttatggcag tcagccacaa gggcttcaaa atccggatga atatgaccgg
    18781 ctgcttttgc agcgcacaag ccggccagtg acccttcaac catagcgctg cttgcctctt
    18841 caataccggc aacgtctccg gcaacataaa taccgtcaag gctggtcatc aggtttttat
    18901 ccctaagggg aacatgaccg cttaactctg aaatatactt catctcacag cccatctgcc
    18961 atagaagctc tgccagcggg gtaagtcccg ttgcaataca cagcacatca acctctatat
    19021 caaactcggt gccggggatt tcttttttat tttcgtcaag cttaatcagg gttatccttt
    19081 ccagcttatc cttcccatgg gcatatacga ctttgtggga aggcattaca ggaacgccca
    19141 gtcttctcag ctttgaagca tgtacggcat agccgccgaa tttcgggcct ctgcatatta
    19201 caccttttac ttttattccg gcctgcataa gctgatagct tacaataagt cctatattgc
    19261 ctgctccgac catgaataca ctctctccgg gagctactcc atagacattc attaatgtct
    19321 gcacggctcc tgctccataa atgccgggta aatcattatt ggggaaagcc tggaattttt
    19381 ccgaagcacc tgtagcaata attaaagtat caggctttat ttttatgtat ttgcccccct
    19441 ggtcaatagt cagtactctg tcttcataca gtcctattac agttgcatca agcataacgg
    19501 taattttgtc cttataatct tctaatttgg atatgaggat atttgcaata tctatacctc
    19561 tgtgtgaggc atattgtttc tgggaaccaa aaaacatatg agtttgtttt atcaactgtc
    19621 cgcccagctg gctgtttcta tcaaggacta taacctttgc accggattcg gcagccttta
    19681 ttgctgcgca aagtcccgca gggcctcctc ctactatagc tatttctgta tgaatcactt
    19741 aatatccccc cgtcctattt gagtttccac tgccattccc tctttgagct tttccacaca
    19801 aactcttaca ttgggcttgc cgttcaccac cataaggcag gaggaacaat ttcctattgc
    19861 acagtaaaag cctctgggtc ttcccagctt gtgggtatgg cccagaaccc ttattccggc
    19921 atcgtggaga gcagctgcaa tcggctcacc ttcatatccc tcataaacct ttccgtcaaa
    19981 tgtgaactga acctttctgc ctttgttgaa cgtaataata ggatgttgct caatcctcat
    20041 aaaggattcc ccctttcgct aattaaaaaa ttatatagca atttatatgc caacgcagga
    20101 aacaattcat tttactaagg tctgtatatt tatgtgtcaa tatattgaca gttggattgg
    20161 ttttagacac aatagatgcc aatatattga cgcgcacgtc gaaatattga cgttttcggg
    20221 agaatgtcat ttttccaggt tatgcttttt aagcttgaaa tacaaggtgc ttctcggtat
    20281 attcaggagc tttgccccat gtgccttatt gcctcccgaa atcctcatga catttatcaa
    20341 ggcttctctt tctgcgtttt ccagtatggt attcaagtca tatatactgt cctgcttcac
    20401 ggcagagatg gtttttctga ttcctccgag gctttgggga agaaaagagg aatcaattac
    20461 ccctgtgttg gaattcctcg ccagcaccac gattctttcg acggcgtttt tcaattctct
    20521 tatgtttcct tcccagctgt gatttacaaa gctgtccaaa acctcaggag taatgccaag
    20581 gatattgatc ccgtattcca tgcagtaatc ttctataaat ctgtttacaa gcaatggtat
    20641 atcatccttc ctttctctta aaggaggaag gtttataaca acggagttaa gcctgtaata
    20701 aaggtctttc ctgaacaatc ctgcctccac catttccgat aagttcttgt tggtggcagt
    20761 gatgattctt atgtttgttt ttatggtttt ttcgctgcct attctggtca tctctccatt
    20821 ttcaagcacc cttagtattt ttggctgcat actcagcggc atgtccccta tctcatcaag
    20881 aaaaagcgta ccgttatgtg ccagctcaat tttgcctatc ttgcctgttt tcagtgcccc
    20941 ggtaaaggct cccgctgtat atccgaataa ttcgctttca attaattcgt gaggtatggc
    21001 actgcagttg atgggtacaa aataaccttc ccgtccgctt tccatatgta ttgcacgggc
    21061 gaacagttcc ttgccggtac cgctctcacc ggttatcagc acgttgatat tggaccttga
    21121 aatatctctt gccagtctga tgctgtctct gaaagctctg ttgttgccca gaatctgtga
    21181 aaatgagtat cgatttttat tgattatgga catttcatgt tccaatacat taagattgga
    21241 ttgggccttc atcagcagct ctgaggtctt tatgaactct gtggcatccc gatccaggct
    21301 tatgcctccg acaaggcggc cattcatgta tagcggggaa gcgcttatga cattgtaaca
    21361 cccttctctg ggattgttgt aaatattgtc ataagatttt ctttccttaa ttatcttagg
    21421 gagcagggag tttgggaaaa attcctttat atgcctgccc aatatcttat ccttgccgat
    21481 attgtaaagc ttttctgcgg cattgttcca gtagcgggtt atacagttct cgtctaccac
    21541 acaaatagcg tcgttgatac tgtcaagaat tatctttaaa agttctccgt cttctacgtc
    21601 aaatatatca aattccttta tttcattatt atccggcata tcaacacttc ctcgcaaaga
    21661 ttttcaacct ttaatatata tactactatt ttcacccttt acattcaact gtacaatagc
    21721 agaatgatgt gtatattgac aggtcaaatg ccataatata taataagctt aattcaaaac
    21781 ctaaggcaat gaagaggaat agtagataac aagtcgcctt aagagaggaa ggcttaagag
    21841 ctgaaatgct ttccaggtta gattttatcg aacccaacct ccgagccggg gatgaactcc
    21901 cgccgtatgc ctgcgttaaa ggctgaagag gaatatacgt ataacggcgt atgttcaaat
    21961 agggtggtac cgcgagtaaa acttcgtccc tttcatgtga cgaagttttt tatttgttaa
    22021 tatcaattta ttcagataag gagagattat tatggctgac aagaagcagc aggaatttgt
    22081 taaagagatt accccgatgg aagaggattt ttcccagtgg tatactgatg tcatcttaaa
    22141 gaccgatatg gttgattatg ctcccgtaaa aggctgcatg gtaataaagc cttacggcta
    22201 tggagtatgg gaaggaattc agagagtagc cgacagaagg ttcaaggcta caggtcacaa
    22261 aaacgcatat ttcccgcttt tgataccgga aagctatctg aaaaaggaag ctgaccactt
    22321 tgaaggattt gctcccgagg tgctgtgggt tacccacggg ggcagcgagg agcttaccga
    22381 gagattgttt ataaggccga cctcggaaac tataatatgc gatatgtatt ccagatgggt
    22441 gcaatcatac agggatcttc ccctgttgat aaaccagtgg tgcaacgttg tgcggtggga
    22501 aaagactaca aggcccttcc tcaggacggc agagttccta tggcaggaag gccatacggt
    22561 acatgcagat gaagaggatg cccagaggga aaccttgctg atactggaaa tatacaggca
    22621 ggttgcggaa gacgaactgg caatgcctgt gatacttggg caaaagagtg aaaaggaaaa
    22681 attcgcaggg gctctccgca cttatacgat ggaagcgatg atgcatgacg gaaaagctct
    22741 gcaggcgggt acatcacata atctggcgca gcatttttca aagatgtttg atataaaatt
    22801 cctggataag gatggcgtgc ttaagtatgc ccacagcacc tcctggggga tttccacaag
    22861 gcttatcggg ggaatcataa tggtccatgg ggacaacaga ggacttgtaa tgcctccgag
    22921 agtggctccg gtacaggttg tcattattcc tatagctttc cataaggaag gagtgctgga
    22981 taaggcaaag gcagtgaaag atgaactgga aaagtgcttc agggtcgaaa tggacgacag
    23041 ggatacctac agccccggct ggaagttcaa cgagtgggag atgaagggtg ttccgataag
    23101 aatggagata ggccccagag acatagaaaa caaccaggtt acaatagcca gaagggatac
    23161 tttggagaaa ttcaccgtat ccatggacaa tctgacagag accgtctccg ggcttctgga
    23221 tgagattcat accacaatgt tcaacaaagc gctgaagaat atggaagaaa agacctatac
    23281 cactacggat tatgaggaat tcaaaaaaat aatgcaggaa acgccgggct tcgtaaagac
    23341 aatgtggtgc ggcagcaggg aatgcgaaga taagctcaag gaggacaccg ccgctacaat
    23401 aaggtgcata cccttccggc aggagcatgt tggagataaa tgcctgattt gcggaggcaa
    23461 ggcggaaaaa atggtttatg cggcaaaagc atattaattt attaatggca tgtatatcgg
    23521 cttattgata tacatgccat ttaatactaa tgctatagcg tttcaacgaa gcagggggcg
    23581 tgcttacccc ctgtgggaca tcttttgcct gattatattt atcagcggaa gtcggttctt
    23641 atcccgaatc tgctgttcct tctgatttcg gacagagtct ggtcttcaag atactgtgcc
    23701 tttgcggcta cttcgctgct gtcaaggatg cccttttcta tcagaagctc aataatactt
    23761 gttattgcca gtgtgttttt gtagtctatt tcctttaaat ctgcaatttg accgatcata
    23821 tccagctttg ccataagagt acctccccgg ttattatatg ttttggccaa gttctattaa
    23881 gagtattgcc tggtgcaggc atattataca ttttatagaa gcggatattg caaaaatgca
    23941 taattagtag tagaatagtt taaaatatgt ataatacata aaatgctttg aagggaaaag
    24001 ctataggtgc gccatcaaga gagcgggcgg caggtgcaag ccggctggca gcttaaagcc
    24061 atccatcccg gagcagtctg cgaggagcag taccggtaca gccgttaaac atatgagaga
    24121 gcagtgaaat tggttactgc taattagggt ggcaacgcgg aaagcctttc gtcccttatc
    24181 atggggaagg aaggctttta tattttctaa tcaagaaaag gaggaaaaca ctatgctgga
    24241 tatcagagca atcagatcca atcctgagga ggtaaaaaga aagctcacaa gaagaggaca
    24301 ggagttcagg attgatgagg ttctgaagct tgatgaacaa agaagacagg ttatttatga
    24361 ggtagaagag cttaaaagca gaaaaaacaa agtttccgaa gaggttgcaa agcttaagag
    24421 agcgaagcag gatgccgagg ctgtagtttt ggaaatgagg caggtatctg aaaagataaa
    24481 ggaaatggat gagagggtaa gaaacctgga tgaggaaata acgggcattc tccttaccat
    24541 acccaatact ccttatgacg gcatacctga cggcagctcc gatgcggata atgtggaaat
    24601 ccggaagcag ggagaaccga ctaagtttga atatgagcca aaggctcatt gggatatcgg
    24661 cgtcgatctc aacatactgg actttgaaac cgcaggaaag gtgacgggag caaggttcac
    24721 cttttacaga gggcttggag caaagctgga gagagcattg ataaacttca tgctggatac
    24781 tcacaccgaa aacggctata cggaaatatt ccctcctttt atggttcaca ggagaagcat
    24841 gacgggaaca ggccagctgc cgaagtttga agaggatgcc tttaaggttc agaatacgga
    24901 atatttcctt gtacccactg cagaagtacc tgttaccaat atgtacaggg agcagatttt
    24961 agacggcagc atgctgccta taaggcatac ggcatatacc gcctgcttca gggctgaagc
    25021 gggttcggca ggaagggata caagaggact tataagacag catcagttca acaaggttga
    25081 gcttgtgaaa ttcacaaatc ctgaaaattc ttatgaagaa ctggagaagc tgactgagga
    25141 tgcggaaagg atattgaagc tcttaaagct tccttacagg gtggtttgcc tgtgtgccgg
    25201 agatttaggc ttttcttctg caaagaccta tgatttggag gtttggatgc cgagctacgg
    25261 aaggtatgta gaaatatcct cctgcagcaa ttttgaagat ttccaggcaa gaagggcaga
    25321 tattaaattc aggcccgaac ccaaggaaaa acccagattt gttcatactc tcaacggctc
    25381 aggcttggct gtgggaagaa cagtagcggc catacttgaa aacttccagc agggtgacgg
    25441 aagcgttata atacctgagg ttttgaggcc ttatatggga ggtctggaca aaataacaaa
    25501 ggcataagaa atttttgaaa agcgtgtcaa ttcgacacgt ttttcattgc tcattgtaat
    25561 gcctgtaaaa atttgataaa attgtaatag gcatttgtca aatattatga gggggtgatt
    25621 caaaaatgtc cggaaaagaa gtaaaaaagc ataaacattc tactttatca gggtatatag
    25681 ttcaaacact ccttatggtg acaccgcttg ccggaatagc cggcacgctg atgtccattt
    25741 atatcgtcag gctggaaggc cttcagctta aattgtccgt ggcttgctac ctgttagccg
    25801 gaataatgtt aggtattatg gcatccttca ggaattattc gaagtttata aagccgctga
    25861 atgaaataag caatctggca gataccctta agaacagcaa cataacatac cggattgaca
    25921 cgagcaaagc aggagggcaa ttagaaataa tagaaagcct aaatgaatcg gtatcaaatc
    25981 tgagaagcac agttaaagat gcaatggaag taggttcgca ggtatctgca gcccctttgg
    26041 aaatgaagaa atcgattcaa atactgagca gtatatccga acaggcagca gctgcgctta
    26101 gtgagcttgc caagggtgct gttgagcaag caggttcaat agaagaaata agcaattcta
    26161 tcagcggtat agtaagcgga ttaagcaaaa taactaccga catggatgat acggataagc
    26221 tggcggagaa agccattgat accattaagc tgggagaaaa ttcagtaaat tatcaggaag
    26281 taaaaatcaa tgagaacaaa gaatttgtca caagcgtgag cagtgccgtt tcggcactgg
    26341 cagagaagtc taaagaaatc ggggatatat tattggtaat caacggtata gccgagcaga
    26401 caaatatgct ctctctcaat gcagccattg aagcagcaag agccggtgaa cagggaaagg
    26461 gctttgcagt agtagcaaat gaggtaagaa agcttgcaga gcaatccgga aactcagtgc
    26521 aaaaaattga agaattaatc agagaagtac agtcgggtat agaaaaagca gtgcaggaaa
    26581 tgactaaagt tgaaagcatt gcagatgaac agcaaaaagc catgtccgct acggtaaaat
    26641 cttttgccgg aatatttgaa gcagtgtcgg aaatatctgc caatataaga gaggttaatt
    26701 cagaatcagt aaatatagca aaaaatgcca ggcaagcaga aacagcaatc ggacagattg
    26761 tcaagatatc agaacaagct gctgctgcaa ccgaacaagt atctgcttca actgatgaac
    26821 aggcaaatgc ggttcgaagc atattggagt catccgagaa actatccgag cttgcaggaa
    26881 ggttaaaagg gagtataaac aatttcacgg tataagagca tttaaataat gaaatgataa
    26941 tagaattgtc tgtaccaagt ttatgatgaa gtgtaataag cagactgtat atcataatat
    27001 ttgtttattg acaccaaaaa ttaatattgc tataatgtat attgttcacg ttgggagaga
    27061 tgttcgagtg gtttatggag ctggtcttga aaaccagagg cgtgttacag cgtccgaggg
    27121 ttcgaatccc tctctctcct ccattttaat aattaatcag acagaataga aagagagggt
    27181 gagaaccctc aaataaatgg ccaataagga aatattaatc aataattatc aatggccaca
    27241 gacaatggag atttacggac agagattggc agccggccat ccggcttcga atccctctct
    27301 ctcctccatt ttaattttat aaaaattttt taagtagtcc aaggagaagt actcaagttt
    27361 ggtcgaagag gcgcccctgc taagggcgta ggtcgggaaa ctggcgcgag ggttcgaatc
    27421 cctccttctc cgccataaag aaattaactg ccgaaaggca gtttatttgt ttatgcagaa
    27481 ggagggtatg agaaccctca aagaaaggcc taaaaatcaa accttcttca aagccgcgtc
    27541 aaattcctcc agcacgtcgt caatattctc aaggccgacg gagaatctca gtaagcccat
    27601 tgagatacca acgtttttca gctcctgagg agaatacgca cgatgagaag tttttgccgg
    27661 gtaggagatg gaggtggcaa caccggcaag gctgggaaca aacttgatgt tcttacactc
    27721 acggatgaaa gtgcaagcac ccttttcacc gccggcaata tcagcagaca gcataccccc
    27781 gcaacggccg tatgtaaagg attcctgagt ccgtttctta tagggggagg aagcaaggcc
    27841 gggatagtag accctctcaa ttttggaatg cttttcaaag aactccgcca ccttcaaggc
    27901 gttggcagaa tggcgctcca tgcgcatgtc caaagtgcgc aggctcctgg tcagcagcca
    27961 agcgtcgaag ggccccataa tggcaccgta cagggtaagg aagtggtgga tgtgagcgat
    28021 gttctctgca tcagacatca cggcgccgcc aataatgtcg ctgtggccgc ctaagtactt
    28081 cgtcgcgctg taaaccgaaa tatctgcgcc cagtttcagc ggctgcgcta tggcgggagt
    28141 tgcaaaggta ttatctacta tcagcttgca gccatgcgaa tgcgcaattt tggagattga
    28201 ggggagatcc tccaccgcca ttaaaggatt ggagatagtc tcggtgtaaa gaatctttgt
    28261 attggaacga atcagagatt caatatcgtc tttttcaaaa tcagcgaagg ttacctcgac
    28321 gccaaaacga ggcagttcat ttttcagata atcatataca ccgccataca gcacaggaga
    28381 tgaaataata tgatcgcctg cttttacctg agaaataata gtggtgacga tggcggccat
    28441 accggaggaa aacactaagg cgccgccgca gtcttccgcc tgggccaaaa cctcttccac
    28501 acagtcagtg ccggggttcg tcatacggga atagacataa ccctgggcag taccggcgta
    28561 cacggcatcc agcgaatcca catcgtcaaa ggaaaataca ctgctcatgt agatcggaat
    28621 aaccttcggc actgagacag atgcggcagt tttctttgct attgcattct cgccggcatg
    28681 ggttagtcga gtcatcattc cttttccttt cataatattt ttcctcctca taaatttacg
    28741 gctttaggct tgaatgccgc ctcatacaat tattatatca tttaaatggc gattaattgt
    28801 caatgacatt acagcagctg catacataga ataaaaatga aacgaattag tagaataaac
    28861 tatgatataa tgtgtaaagt ggggtgagag catgaaaaag ctgaaattca tatataatcc
    28921 ttactccgga gaaggtgttt tcaaggacag gcttgattat ctgattgaaa ggtttcaaca
    28981 agccgggtat atgatggttc cttttagaag cacaaatatc aatacacttg acagggcttt
    29041 ttttgacata catgaggatt atgaggctgt ttgcatttca ggaggcgacg gtactttaag
    29101 cactgttaca aatataatgg cgtcaagaaa gctgaagctt cccgtcggga tatttccctt
    29161 cggtacagcc aacgattttg caacacacct taatataccg ggagatattg aagcctgctg
    29221 tgaaataata gaaaagggca ggacaaaaaa agttgatata ggaagggtca acaacagcta
    29281 ctttttaaac gtatgcagtg cggggctgct ttcggatgtt gcctatgaaa cggatacgaa
    29341 tttgaaaaat gccctgggaa aactggctta ttacatgaaa ggcatagaga agatacccag
    29401 atttactccg ttcaaaatga ggcttcagta tggaagcaat gtaatcgaag acaactttct
    29461 gctgctcctc atattaaacg gcagatctgc gggaggcttc agcaggcttg cacccaatgc
    29521 ggtgatagat gacgggctgc tggacgtaat agcggttaaa aacacaaata taacaaatat
    29581 acttgcgtta tttttgaaaa tattgagggg agagcatata ggagacacca acctctataa
    29641 ttttcaggcc gacaggcttg ctattacctg tgaaggcggc tgtgaaaccg atattgacgg
    29701 agaacggggg ccggacttcc ctttggatat agaggtcaag aagcattttt tgaaggtatt
    29761 tgtaccgtaa tacgggacac gggggacgtt tcgggacacg gggacgtttc tcctgtcccg
    29821 ctgaaagcgg gacaggagaa acgtccccgt gtcccgaaac gtccccgtgt cctcgcgtca
    29881 cattatcctt cgtatatata atacagataa tcgcaggttg aagggtccat cataccgaat
    29941 cctgcagcat tgagggcctc aatcagttcc gtatctgccg gcataaccga gaccagaggc
    30001 ggaacggggc atttgcccgc aagggccagt aaatagtaat tcataaaggt atttataccc
    30061 tcaaagcttt catcaaacac tatgaaatgc ggatctcctt ttaacttgaa aagccccttg
    30121 tgcccgttta tcgatacgaa gcatttttcc ttcagcagct cttcatacag ctccggagtg
    30181 ggtttctgaa aaaggaacac aaaaggcagg atgccgtctc ttcttttaat atagggttga
    30241 tttgcaaact cgtcatagga aggcttccag ctttctgcgg caaagtctcc gatgctcaat
    30301 gaattatctg catcgctgta gtccttccaa accattttat actcctgttt cagcctaaaa
    30361 ccgagttttg tcatcaaacc gatgctttca accgagagag cactggtgga aaatgctatt
    30421 ttatttattc ttccgcttcc aaggtcctcc aaggccttat taaaggtcct gacagccagc
    30481 ccctttccta tgccgcgccc ccggtaatcc ttatgggttc tcaagccttc aagccagccg
    30541 ctgccgtcgt aaagcacaga atacttgtct gttcctatta ccttgttggt atcggtatca
    30601 acggcaccaa gaaacaagcc cttatcatca acccatttat ggaaaatctc agggagataa
    30661 tcggttccat cccagatatc cttacagata tccaatatat catcataatc ttcatgagca
    30721 agctttctgt attctatcat acaattaccc cttctatatc gtaatttttt ctatattttc
    30781 cggcaaatag gctatataca attatactat acgcatatat atctttgcac tgattgcata
    30841 tatattttta tgaaaaaaat gataaaatgg tattgtatcg agattagtaa aagctcaaat
    30901 atttacgtat atatattgta cgaaggagga atgattatgg aagcggaaaa atacaaggtt
    30961 gcggaaaccg cagtggagga agctgacctt tccaagctta ctgaagagca gaaacagagg
    31021 ctggaagaaa taataaaaac tattaatctg gaggattccc agttcatact tcagtacggg
    31081 gtgggggccc aaagccagat agccggtttt gcggataaca ttgtaagcga ggtaagagca
    31141 aaggacagcg gttatgtggg agatatactc accgacctta tgcttaaggt aaaagaactg
    31201 gatgttgatg gtgtaagctc gggcaaggga ttcatgtcca ggtttcccat tttcggatct
    31261 ttagttaaca atacccagaa gtttgccgca agatatcaga agctcagcac cgagattgag
    31321 aaaatagtag atgaactgga caaatccaag atgcagctgt taaaggatat caccctgctg
    31381 gattacctgt ttgagaaaaa ccttgaatac ctgggcaacc tcaattatta cataatggcc
    31441 ggaactttga agcttaagga aattaacgaa aagataatcc cggaaatgca gtccaaggtg
    31501 aatgaaacca atgacccttt agatgcgcag aagctgaatg accttataca gatggcaaac
    31561 agatttgaga agaaaataca cgacctgaag ctcagcagga tggtgtccct gcagacagcg
    31621 ccccaaataa ggcttattca gaacaataac caggtactgg tggagaagat acagagttct
    31681 atacttaata ccatacccct gtggaaaaat cagatagtta ttgcaataag cctggcaaag
    31741 cagaaaaatg ccctggcggt gcagaaggaa gtaaccgaca ccaccaatac cctgcttcag
    31801 aagaatgcgg agatgctgaa gaccggttcc attgacctgg caagggagac tgagcgggga
    31861 gttgtggaca tagaaactct taagaaggta aatgaggatc ttatagccac aattgacgag
    31921 accctgaata tccagaagga aggccgcatc aagaggaagc aggcggaaga agagcttgcg
    31981 gctatagaaa aagagcttaa acaaaaactg atcgggaaat gatatatata aaaattgagt
    32041 ggaggttatc caatgaatca gaagtggagt ctggatgaac tgtataaatc ctttgagtca
    32101 aaagagttca agaaggacta tgaaaagtgt atcaaggata ttgagaggat aaaggaatgg
    32161 gctgagagca attttaagga ctcatcagat gcagcccata aggtgagaag atatataaag
    32221 accataaatg agttctatat gctgtattca cggctttaca gctacgcaca gcttaccgtg
    32281 agcgtggaag ccagaaatga aaaggcactg aaagtaatcg actgcctgga ggaaaaagcc
    32341 acagagctta caaagcccga agtaagcttc cagaaatggc ttggaaccat aagcaatata
    32401 aatgaaatca ttgcctctga cagttacctg aagaagttcg agtttttctt cagggagaat
    32461 ctcgaaaaga gcaaatatct gctaagcgac aaagaagaag cgctgatatc aaaaatgaag
    32521 actacgggtt cggctgcatg ggaaaagctt caggagcttt taacctccac acttcttgtg
    32581 gaaattacat tggacggaga ggataagaag cttccgctgc cgataatcag gaacatggca
    32641 tacaataagg atgctgagct tagaaagaaa gcctatgaag cggagctgaa ggcttatcaa
    32701 aagattgacg aatctgtggc cgcctgcttg aacggtataa agggagaagt aattaccgta
    32761 tcgcggatga aggggtacaa gtcacctttg gagaagacga ttctggattc aaggatggat
    32821 ttggaaacac tgtatgcaat gattgccgca atgcgggaaa gcctgccgga ctttcacagg
    32881 tatttcagga aaaaagcaga gcttatggga tataagaacg ggctgccttt ctatgagatg
    32941 tttgccccca tgggggacgc ggatatgcaa ttcagctaca aggaagccag agactatata
    33001 gttgccagct tcagaacctt cagcgacaag ctggcagatt atgccgccca tgccttcgat
    33061 aaccgctgga tagatgcgga accaagagag ggcaaacggg gaggggcttt ctgtgagaat
    33121 ctccattcca taggggagag cagaatactc acaaactttg acggaagctt cagcaatatg
    33181 accaccctgg ctcatgaact gggtcatggg tatcacgggg catgccttat gaaagaagag
    33241 tatctcaact ctgattaccc gatgcctata gcggagacag cctctatatt ctgcgaaacc
    33301 atagtcaaag aagcggcatt aaagacagcc aataaggagg aagctttctc catgctggaa
    33361 aatgatatat cagattcggc ccaggtcatt gtggatatat acagccgctt catttttgaa
    33421 agtgaagtat tcaaaagaag ggaatccggc tcattatccg taaatgaact gaaggagata
    33481 atgcttgatg cacagaagga agcatacgga aacgggcttg acccggactt cctgcaccct
    33541 tacatgtggc tgtgcaagcc gcactattac agcgccgact ataactttta caacttcccc
    33601 tacgccttcg gactgctttt ctcaaagggc ttgtatgccg agtacctgaa gaagggaaag
    33661 gaattcacgg cggagtatga caggatgctg tcggtgacag gaaagatgag cattgcggat
    33721 gttgcaaagg ttatgaatat tgacgttcac gatccgggct tctggagaaa ttctctggag
    33781 cttatagaga aggatataga cagatttata aagcttggat aa
//
LOCUS       DAOOAB010000026        33737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1677_length_33737_cov_14.599697, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000026 DAOOAB010000000
VERSION     DAOOAB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 33737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33737
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1677_length_33737_cov_14.599697"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1460)
                     /locus_tag="PKU88_07170"
     CDS             complement(60..1460)
                     /locus_tag="PKU88_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPX69092.1"
                     /translation="MGGLFGVVSREDCVMDVYFGTDYHSHLGTCRGGMAIWGGSCFNR
                     SIHNIESTQFRAKFEAELSKKSGNMGIGCISDTDPQPLTVRSHLGQYAISTVGRINNL
                     DEIAEKAFSNRNIHFLETNKGIISPTELVSVIIDHEDSFKEGLLRAQELIQGSCSVLL
                     LTPKGIYASRDRLGRTPLMVGSKEGAYCISSESCTFANLGYKSDYELGPGEIVFLTPE
                     VVEQVSPPGDKMKICAFLWVYYGYPSSTYEGMNVEVMRYRNGAAMAKNEDLGIDIVAG
                     IPDSGLGHAIGYSNQSKVPYGRPFIKYTPTWSRSFMPQDQDIRNLVARMKLMPIPEIV
                     ADKRLLFCDDSIVRGTQLRETVDLLYKCSAKEVHIRSACPPIVFGCKYLNFSASRSEM
                     DLAARQAIKELEGKEPDSLEEYCNQATDKYHNMVDLICRKLNFSSLKYQNIHDMIEAI
                     GISKDKVCTYCWNGKE"
     gene            complement(1644..2579)
                     /locus_tag="PKU88_07175"
     CDS             complement(1644..2579)
                     /locus_tag="PKU88_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69093.1"
                     /translation="MEAVISFLHAAIVAGTPLLFATLGEIITERSGNLNLGVEGMMLM
                     GAVIGFMVGLNTESTTLALLAAMIAGAGGALIFAFLTITLRANQVVSGLTLTIFGTGF
                     ADFVGQDLIGQIAPESLRLFFRPYTIPLLGDIPFIGPVLFRQDAFVYLGYAASILLGL
                     YLYKTSTGMNLRAVGENPGAADAAGINVTLYKYLHTLLGGALCGLGGAYLSLVYVPAL
                     QEGVTAGRGWIAVALVIFATWNPYKALFGAYLFGGLDIIGFRIQNLGIDVSQYFIDML
                     PYLVTITVLVVVSMRKSREKAGPKACGIPYFREER"
     gene            complement(2582..3676)
                     /locus_tag="PKU88_07180"
     CDS             complement(2582..3676)
                     /locus_tag="PKU88_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69094.1"
                     /translation="MIMPITIRAIKKSEPGRMKAASIRIGAIILALVASSLFILLLSL
                     NPADVYIKMIQGAFGTAYRIKETIVKAIPLVITSLGIAIAFKMQFWNIGGEGQIAIGS
                     FAASYFALYMNDMPMPLMLTVMILAGMLAGGLWALIPAFFKAQWGTNETIVTLMMNYI
                     ALKWVMFLQYGPWRDPKSLGFPKIANFPQNAVLPKLLGVHVGWVIALILVVVVYIFIN
                     HTKKGYEIAVIGESMNTARYAGINIKKTILIAIMLSGGLCGLVGMIQASAVNYTLSVE
                     ITGGVGYTAIITAWLAALSAPLILIVSILFAALLEGGSYIQTVFGIPQAAAEILQGII
                     LFFVLGSEFFVRYKFVIVKKHSAIAEKGAD"
     gene            complement(3645..5204)
                     /locus_tag="PKU88_07185"
     CDS             complement(3645..5204)
                     /locus_tag="PKU88_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69095.1"
                     /translation="MDNNYISMKNINKSFGKVVANRNINLAVRRGEIHALLGENGSGK
                     STLMNMLSGVYTPDSGSIFIKGKEVQFAAPKDAIEAGIGMIYQHFKLIDVMTAKENII
                     LGRKNSFLMNEKTLSDKIRDMSARFGLDIDPDKKVYNMSVGEKQTLEILKVLYRGAEI
                     LILDEPTAVLTPQETEKLFKIMRKMKEEGCAVIFITHKLNEVMEVADRITVLRKGETV
                     MTVDKADTNPKHLTELMMGCPADLSIKRIETEKNNVAMEVRNLSAVNSEGIKVLKGMS
                     FDIYSGEILGVAGIAGSGQKELCEAIAGLHPVKQGEIVFNGENLVGKSPAEIITKGVS
                     LSFIPEDRLGMGLVASMDIVNNVVLKLYQKQKGIFIDRKPAEQKSVEIIERLSIQTPG
                     IKHPVRMLSGGNIQKVLLGRELDSRPQVLITAYPVRGLDVNSCYTIYDLLNEEKRKGV
                     AILYIGEDLDVLLELCDRIMVICAGEITGIVDARYTTKEQIGLMMTGTVNLMKEGEKS
                     EHDNANNYTSH"
     gene            complement(5224..6354)
                     /locus_tag="PKU88_07190"
     CDS             complement(5224..6354)
                     /locus_tag="PKU88_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX69096.1"
                     /translation="MKRTISILLVLVLVFAAMLTGCAPKAEPAPAAPETPAPAEEPKA
                     EPLKVGFVYIGSAKDGGYTQAHDEGRLYLEQQLGDKVITMYKEDVPESSECEKVINDL
                     VDQGCTVIFTNSFGHMDYTEKAAQAHPDIKLYHCSGYKSGPNFSNYFGAMEEARYLAG
                     IVAGLRTQNNTLGYVAAFEIPEVVRGINAFTLGAQSVNPKVKVKVNWTHTWYDPAKEK
                     EAAKALLDAGCDVLAQHQDTTATQQAAEERGVWAIGYDLDTRDSAPKAYLTAPVWNWG
                     KFYAAEVQRILDGKWKQENYYGNMKDGLVDLAPLTDNVAPGTQEAVDKVKAKIIDGSF
                     YVFQGPIKDQTGAVRVPEGKSMTFDEIMSVDWFVQGVEGKIK"
     gene            complement(6442..7011)
                     /locus_tag="PKU88_07195"
     CDS             complement(6442..7011)
                     /locus_tag="PKU88_07195"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HPX69097.1"
                     /translation="MELLKDRILKDGKVIGDSIIKVDSFLNHQLDIELFNEIGREFKE
                     RFSDEKISRILTVEASGIAVACIAAQYFKVPVVFAKKYEALNLDSDTYQSEVYSFTKQ
                     RNYNIRVSKKYITPEDNVLIIDDFLANGKAASGLVDIVLQAGARVAGIGIVIEKGFQK
                     GRETLRQKGVRIESLVIIKSMDEGVIIFD"
     gene            complement(7140..7589)
                     /locus_tag="PKU88_07200"
     CDS             complement(7140..7589)
                     /locus_tag="PKU88_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPX69098.1"
                     /translation="MVVNGKAYSLEVDIRESLLEVLRNRLHLTGVKQGCSVGECGACT
                     VLIDGFPVNSCIYLAVWADGRKITTIEGVSKEGELSKVQQAFVDEGAVQCGFCTPGLV
                     LTATALTESGRKYSDEEIKREISGHLCRCTGYNKIFNAVKRALDVDC"
     gene            complement(7606..8487)
                     /gene="xdhB"
                     /locus_tag="PKU88_07205"
     CDS             complement(7606..8487)
                     /gene="xdhB"
                     /locus_tag="PKU88_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HPX69099.1"
                     /translation="MFDIVNIEKPGTLEAALEMLSRNPELKIIAGGTDVLIKMRNGAL
                     EEAELLSLRNIPGMDEIRILDNGTISIGAMAAFAKIARSSIINENLPVLSEAAVSVGG
                     PQIRNMATIGGNICNGAVSADSAATLLAYNAELKLLSRSGSRTVPVSEFYAGPGKVNL
                     LPAEILTDILIPEAGYGRTSGCYIKYSGRKAMDIAMLGVSVVCKTEGGKFSDVRIALG
                     VAAPVPIRCSEAEAYAIGKEISDKTMKETGRYAVKASRARDSWRASKAYREHLIEVLV
                     FRALKEAVIRGGGVDIG"
     gene            complement(8503..10794)
                     /gene="xdhA"
                     /locus_tag="PKU88_07210"
     CDS             complement(8503..10794)
                     /gene="xdhA"
                     /locus_tag="PKU88_07210"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HPX69100.1"
                     /translation="MAFEIVGKRTIRKDAFAKVTGRAKYADDFFEGDMLVGKVLRSPY
                     AHAIVKRINTSRAKALPGVEAVFTYEDLPKLKYATAGHPWSLEPDHRDAADRLILTDK
                     ARYVGDGIAAVVASDAFTAEKALKLIEVEYEVLPFVLDPEKAMLPGAPVIHEEKPDNI
                     LGDASEEIGDIEEDFGKADHIFEGVYETSIVQHCHLEIQSAYAYADADGRIVIVSSTQ
                     IPHIARRIVAQALGMPWGKVRIIKPYIGGGFGNKQDIIVEPIAAAMSLAVNGRPVRYA
                     LTREECFVDTRTRHAMKFYMKTAVTKEGKLLSLHMRPISNNGAYASHGHTIAMSAGGK
                     SRLLYDFNSMKYEPKTVYTNLPAAGAMRGYGCPQIFFALESHIEDIAREMKMDPIEFR
                     KRNLISAGKTDPHSEIVVRTFGLPECIEKGKQLIKWDEKLEKYKEQKGTKRRGLGMAC
                     FSYASGTYPQGFEISGARIVMNQDGSVQLQVGATEIGQGSDTVLSQIAAEVLGLPYDM
                     VHIVSQQDTDISPFDLGAYASRQTFVSGAAVKKAAEEVKSKVLEMASEKTGLSIDELD
                     IKAARIVERKSGNEICTLEDIAMGSYYDRLNAEPICGNASVNVRTNAMAYGVTFAEVE
                     ADMETGRIEVIEIYNVHDSGVIVNPMLAEGQVHGGVSMSLGYALSEQLLFDPETGRPL
                     NNNFLDYKLQTIMDTPDIGSAFVQLHEPEGSVGQKSLGENTTISPAPAVRNAVLNATG
                     IAFNKLPMTPQKVFEKFKQSGLL"
     gene            complement(10829..12157)
                     /gene="ssnA"
                     /locus_tag="PKU88_07215"
     CDS             complement(10829..12157)
                     /gene="ssnA"
                     /locus_tag="PKU88_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HPX69101.1"
                     /translation="MILVGNGRVITRDDDIPIIENGCIAIDGNKIAELGLTSELKEKY
                     SGARFIDAKGKLIMPGFINTHMHYYSTFARGMSNDSPPATKFSEILKGLWWRLDKVLT
                     LEDVYYSAVGPMIEQVKCGVTTVIDHHASPYAVKGSLFKIAEAAEHIGIRSNLCYETS
                     DRDGEKIAEEGIAENVEFIKYCSNKKDDMLKGMFGLHASMTISEKTLDKCLDAISGLD
                     TGFHVHCAEGIEDLEDSRARYGKGVIERWYDSGVLGSKTIAVHCIHISEKEMDMLKEK
                     DTIVVHNPESNMGNAVGASPVPEMFGKGILLGLGTDGYTADMMESYKVGNIIHKHVKG
                     DSNVAWGEIPAMLFANNRKIVERFIDGQVGILKAGAMADVIIVDYNPPTPINEGNING
                     HLLFGINGRFVDTTIVNGRILMEDRKLVNIDEERMMARSRELAREVWKRF"
     gene            complement(12174..13550)
                     /locus_tag="PKU88_07220"
     CDS             complement(12174..13550)
                     /locus_tag="PKU88_07220"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HPX69102.1"
                     /translation="MSQKLLKNIDLIVTFNDREETFEHCDILIDGTAIREIGSGLDTA
                     GDAEVIDCSGLTALPGFVNTHHHLYQTLFRGIKEVQEKPLFPWLVGLYEFWKNITPEA
                     VYYGALVGFAELLRTGCTLTSDHHYVFPKNQPGTLIDDQIRAAREIGIRFHATRGSMA
                     LGRDMGGLPPMEVVQAEEAILADSERLINKYHDSSPYSMQRIALAPCSPFSVTRELMV
                     QTKELAREKGVMLHTHLAETLDEEKFCLERYGRRPFELMEDLEWVGDDVWFAHGIYLN
                     DGEIKRLQGSGIAHCPSSNMKLSSGICRTTEIIRAGGKLSIAVDGSASNDGSNMWEEV
                     RRAYLLNHLKYGTDGLTAYQVLRTATRGGAEVLGRDDTGCLDIGKAADIVLFNLKGIE
                     YSGCHDPLVSLICLGNSSMVDMTVVNGRVVVKDGKLTTIDSDRVASDAHKTAVEIVKR
                     QRNSIRNH"
     gene            complement(13872..16889)
                     /gene="ygfK"
                     /locus_tag="PKU88_07225"
     CDS             complement(13872..16889)
                     /gene="ygfK"
                     /locus_tag="PKU88_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HPX69103.1"
                     /translation="MSDRMRLIPFEKMLSWVLSELKEKDSIFGIHKSKFYKNKSGSFT
                     ELFGEKLATPLGPAAGPNSQLAQNIVAAYLTGCRFIELKTVQKLDGEDLPVSKPCIYA
                     QDECYNVEWSTELRVPDAYNEYIKAWFLLHVLMKELGLSEKRDFMFNMSVGYDLEGIK
                     SPKMDAYIEGMRNAANTDIWKECRKVLLSAMNQFTHFTREDLDMISPVVCPSITLSTL
                     HGCPPQEIERIANYFLKEKKLHTFVKMNPTLLGEKFVRDTLDAMGYGYITLNGHHFIH
                     DLQFPDAVAMLKRLKALARELNLEIGVKLTNTLPVKILNQELPGEEMYMSGRSLYALS
                     ISLASKLAKEFNGDLHISYSGGIDFFNIDKVMEAGVRPITFATTILKPGGYERINQLG
                     HKAEGLQKRMAAGIDIAKISSLAENALTDKHHLKELRPVNSRKLTSELPLYDCAVSPC
                     AMGCPINQQIPQYVALVGEGKYDEAFEVIVSDNASPAITGSICDHQCQYKCTRLDYDE
                     SVLIREMKKTAALKAQDKYTAGIRPAEIRSNKKAAIVGAGAAGLATALFLRRNGMEAV
                     VMDKRERPYGVVEYVIPEFRISSDMVRKDLELVKRSGVGFKLGINENFSVEALKKEYD
                     YVVLAIGACKPGQISLEEGAEKTVNAISFLENYKAQKGNMKLGSHVCVIGGGNVAMDA
                     ARAAKRVPGVESVSIVYRRTKALMPADREEIHLAASEGVLFRELLSPAAYKEGKLVCE
                     VMALGDRDSSGRRSPVPTGEKITLDADTVIVAVGEQVDSIFLAKNGIELDSKGFPKVS
                     SACETNIPGVYVAGDAKAGPATIVRAIADGKAAAKDILSKEGLKADFEKKVLPVDEKI
                     LYERKGILNNPVYSDKEAERCLSCENICELCVDVCPNRANAVIFVDEGFASGHQVIHL
                     DGMCNECGNCGIFCPHEGNPYKDKVTVFWNEEDFRDSNNKGFLVTDMAACKCLVRTEE
                     GQIVNYALGQENGVSKEMAAIIKACIGKYSFML"
     gene            complement(16939..18183)
                     /locus_tag="PKU88_07230"
     CDS             complement(16939..18183)
                     /locus_tag="PKU88_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPX69104.1"
                     /translation="MSYDFKQILELAEKYKPQMSKFLRDMIAIPSESCGEKEVIGRIA
                     EEMRAVGFDKVELDPMGNIMGYVGHGKHLIAMDAHIDTVGVGNIKNWEFDPYKGYEND
                     EIIFGRGASDQEGGMAAMVYAGKIIKDLKLEDDFTLLITGTVQEEDCDGLCWQYIIKE
                     MNIRPEFVVLTEPTSCRIYRGHRGRMEIKVATQGVSCHGSAPERGDNAIYKMAPILME
                     LKELNERLISHPFLGKGTLTVSEIFHTSPSRCAVADSCWISVDRRLTAGESGEYALNQ
                     IRELPSVKAAKAEVTMYPYSRPSYTGLEYPTEAYFPTWFIEEDHPVCSTLLEAYKGLF
                     EDEPVLDKWTFSTNGVSIMGKYGIPCIGFGPGHEDQAHAPNEKTWKDELVKAAAMYAA
                     IPSLYVKKYADKMPKTTENLAD"
     gene            complement(18195..19676)
                     /locus_tag="PKU88_07235"
     CDS             complement(18195..19676)
                     /locus_tag="PKU88_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPX69105.1"
                     /translation="MRKLIDFTINEKGLEKAVQNVKERNIVIPTFAQMKDPDKIPAKV
                     KEKLKNTGLWDVDPINLFRISWKNEPVKHGGLYNKLPNYIEIPREISGVKARIFAMAG
                     KFFPTGAHKVGASFACLVPRLVTGQFDPTYHQAVWPSTGNYCRGGAYNSTLLGCSSIA
                     ILPENMSKERFEWLKTVAGEVIATPGCESNVKEIYDKTWDLRRTRDNVVILNQFGELG
                     NHLWHYEVTGHAMEDIINAEAGSKGKVAGVCLTSGSAGTLGAGDFIKDRYPNAKLAVG
                     EALQCPTLLSNGFGDHRIEGIGDKHVPWIHNVKNTDMVIAIDDNDSLGLFRLFNEPAG
                     QEYLKSLGIRDEVVEKLSWVGISGAANIISCIKFAKYYELAENDIVMTVLTDSAEMYQ
                     SRLMEMEEARGRKYSNVDAAIDHNRYVLALGTDTMEELTYQSRKRIHNLKYYTWIEQQ
                     MYDLDELNEQWYDYDNYWGRLHKMAPELDSLIEEFNKRTGLGY"
     gene            complement(19730..20965)
                     /locus_tag="PKU88_07240"
     CDS             complement(19730..20965)
                     /locus_tag="PKU88_07240"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPX69106.1"
                     /translation="MSKVLKLKCVKCGREYNEAEILYTCRDCGIDGILDVELDYDSIR
                     KELTREYLKNNKDYSLWRYLPAIPLEKTGGIQPLQVGWTPLYNIKSLSKETGLSSFFL
                     KDDSRNPTASLKDRASAVGVAKAVELGRNVMCAASTGNAASSLSGFAAVAGIKTYIFV
                     PETAPEAKVTQLLIYGSNVFLVKGTYDEAVELCFKAAEEFGWYNRSCAINPYLVEGKK
                     TISYEICEQLDWKAPDIAVVAVGDGCTIGGVWKGFKECFELGLIDKFPKIVGVQAENS
                     NPVTRAFNKKTYEFEYRVPDTLADSISVGIPRNGIKALNALHESGGYMVDVSDEEILA
                     AMKILAQKTGVFGEPAGVTSFAGIMKMKELGLLKGDEKVVSIVSGSGLKDIKSAVKAA
                     GKANHVEPDLNDLKRIVHI"
     gene            complement(20962..23532)
                     /gene="xdh"
                     /locus_tag="PKU88_07245"
     CDS             complement(20962..23532)
                     /gene="xdh"
                     /locus_tag="PKU88_07245"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HPX69107.1"
                     /translation="MHLISFKVNGRAVNVREGTNLLKYLREEERLTSVKNGCDSGVCG
                     SCTVLIDGKAARSCTLKADKLQGREVLTVEGLDGKEKEVYSNAFAEAGAVQCGFCTPG
                     MMLASKALLDKNPDPSEEEIKKAIRNNICRCTGYVKIVKAIRLAAEALRESKHISLST
                     CSSNIGQNWRRVDAIQKALGEATYCDDVFMEDMLYVSVLRAMKPRARVLSVDTDAAKA
                     LEGVKAVLTAEDIPGENYQGYIFKDWPTFVPVGEMTRYAGDAVAAVAAETKELAEAAI
                     RLINVTYEEMEPVTDAKYAMRSDAPKIHEKGNILSRTYVKRGNPEKAMAESKYVVKNT
                     YRTPPVDHAFIEPESAAAFYEVDVLTVYAATQSITHDHHELCRVLGLPHDKVRVILQN
                     VGGGFGGKEDLSAQHYAALAAFYTKKPCKYTFTREESLLCHPKRHGMELEVATGCDER
                     GMLTFMTARIIADTGAYASLGTAVLERACTHVCGPYSIPNVELEGFCVYTNNAPAGAF
                     RGFGVPQSNFAGEANMDQLADLAGIDRWEFRYRNAVEPGMVLANGQVCGDDTAVKETL
                     LAVRDEYYRSRYAGIACAFKNTGIGVGLPDVGRCRIRIKEGKVVVYSAASCIGQGLAT
                     VMIQIVSETLGIDGSDIIFSQPDSLLCPDSGATTASRQTLFTGEACRRAALKLKAALA
                     EKTPEELEGVVFEGEYSGVTDPINSSRENPVSHVAYSYATHLAILDAEGRIAKIVAAH
                     DIGRAINPLNIEGQIEGGVVMGLGSALRERLIYEGGILKSRFGTLGLLRADEIPEIDV
                     KIIEKNQSELAYGAKGIGEISLIPVAAAVASAYQRFDGIRRFELPLEGTPYDRKKVEE
                     "
     gene            complement(23550..24128)
                     /locus_tag="PKU88_07250"
     CDS             complement(23550..24128)
                     /locus_tag="PKU88_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX69108.1"
                     /translation="MSNEVTNKILDAALEIVAQNKISGTRMHLIAKEAGVVQSNVHYY
                     YPTKNDLMIALLDRIQERFTEKRMSSVDLEKKSFEENLRGLFHEKKDDIYNHKKIDYV
                     QFDFWVQGTANPEIREKFIGAFNIWRSNITEVLNQMPGFPECKSEQCAMLPYLLVSIM
                     MGASMQYLIDEGKFNLDAYFDAAENMILNFVK"
     gene            complement(24180..25376)
                     /gene="ygeW"
                     /locus_tag="PKU88_07255"
     CDS             complement(24180..25376)
                     /gene="ygeW"
                     /locus_tag="PKU88_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPX69109.1"
                     /translation="MEEIRKLVSQFKKLDSNAMCNNDFLLTWEKTDDEIHAVFQAAEI
                     LRKMRENNISPRIFDSGLAVSLFRDNSTRTRFSFSSACNLLGLTVQDLDEGKSQIAHG
                     ETVRETANMISFMADVIGIRDDMYIGKGNTYMREVSKAVQTGYEEGVLEQRPTLVNLQ
                     CDIDHPTQCMADMLHLINYFGGVENLKGKKIAMTWAYSPSYGKPLSVPQGVIGLMTRM
                     GMDVTLAHPEGYELLSDVEDIARANAAKSGGKFSKTNSMKEAFANADVVYPKSWAPYA
                     AMEKRTNLYGEGKFDEIKALEKELLAQNAGHKDWECTEEMMKLTKNGKALYLHCLPAD
                     ITGVSCKEGEVAASVFDRYRTPLYKQAGYKPYIIAAMIFLSKVKNVPETFEMLMDSNY
                     PRFSGK"
     gene            complement(25645..27003)
                     /gene="hydA"
                     /locus_tag="PKU88_07260"
     CDS             complement(25645..27003)
                     /gene="hydA"
                     /locus_tag="PKU88_07260"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HPX69110.1"
                     /translation="MDLIIKNGTIITASESYKADIAVKNGRIAMISGNIQAAGERVVD
                     AAGKLVLPGAIDAHVHLELPVGETVSADSYESGTRAAACGGTTMVFDFVTQEKGMGLV
                     ETADSRLGYVASRACVDYAFHIAVTDLTDKVLDEFKASVEFGVPSFKLYMVYKDMMVD
                     DGVIADALVRSKETGALVAVHAENPYIIDRRIKKLLSQGKTSAWYHYESRPEFVEADA
                     VRRMVYLARTFDAPLYIVHLASREGLEEVSGARDEGFEIYAETCPQYLNFNNEVYKRE
                     DGRNFVCSPSIKGEDSRRALWEGLRRGYISTVATDHCPFQSYEKDWGKDDFTKIPNGC
                     MGVENMYPYMLSEANKGNITFNKAVEVCSANAARIFGCAPQKGTLAVGSDADIVIYDP
                     EKKFIVTRENMHSDVDYTIWKDCEMSGYPVMTFSRGRLVYEEGSFLGEPGWGKFVKCF
                     RK"
     gene            complement(27054..28769)
                     /gene="ade"
                     /locus_tag="PKU88_07265"
     CDS             complement(27054..28769)
                     /gene="ade"
                     /locus_tag="PKU88_07265"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPX69111.1"
                     /translation="MSVEQKQKRIEIAAGREKADLAIKNASIVNVFTNEIISGDIAIH
                     DSLIVGVGDYSGREEIDAGGMYVCPGLIDCHVHIESSMITPAEFARVVLPHGTTTIIA
                     DPHEIANVCGIEGIHFMLNEARNVPMNIFYMMPSCVPATPFENNGAALTARVMEPFAG
                     SCHILGLGEVMNYPGVLAGDRDILNKIELYDNAVVDGHSSGLSEHELCAYKAVGVLTN
                     HECTNIDEVIAHLRLGMYVQIREGSAARNLEDIISGLIERDVPLNHCLFCTDDKHLED
                     ISREGHISCNVKKAISLGVDPIEAIKMATINPAKCYHLKRLGAIAPGYSADLLILSDL
                     NSFDIEKVLFRGSVVCEKGKKPDISRIIDDPNVLNTVRIPNLSPEHLKIKIKGDKANV
                     ITIIPNQLITKKERLAVPKDNGNNFIPDEVFSKIAVVERHKATGNIGLGIVKGFGIRN
                     GAIASTVAHDSHNLIIIGDNDADMLLAAKVLKEANGGYTVVSGGEVTGTLPLPIAGLM
                     SDRDSFFVENKLSGMIESAKSLKIPDGIDPFVTLSFLALPVIPELRVTDRGLFDVGSF
                     SFIDI"
     gene            complement(28766..29560)
                     /locus_tag="PKU88_07270"
     CDS             complement(28766..29560)
                     /locus_tag="PKU88_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPX69112.1"
                     /translation="MEYAILEKLAAEVKANRKAALVTLVEEQGSSPGKKGFMMAVFAD
                     GSTLGTIGGGNLENKAREYALLCMSEGKNKLMEMELDDTGELHMQCGGKANIFIKVFK
                     AKDSLVIAGGGHIALELHKLGKLMGFHIVIMEDREEYGNNERFPGCEIHVGDIGECMS
                     KYPIDEDCYVVIVTRGHASDADALRAAIGKGAGYIGMIGSRQKTQFVFNRLLSEGCKK
                     EDLEAVYAPIGLGLGGDSHQEIALSIIAEIMLVKKGGSLKHMRDLR"
     gene            complement(29565..30368)
                     /gene="yqeB"
                     /locus_tag="PKU88_07275"
     CDS             complement(29565..30368)
                     /gene="yqeB"
                     /locus_tag="PKU88_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HPX69113.1"
                     /translation="MDDIIIVKGAGDIATGIGHRLHRCGFRLLLTEIEKPTVVRRFSS
                     FANAVLKGEAEVEGVRAVKAECIEDIYEIWNNSHIPVLCDSEMKILEQIKPLVIVDAV
                     LAKRNTGTYRGSAPVTIGVGPGFEAGADVDAVVETNRGHDLGKVIYKGTACTDTGIPG
                     YIMGYSQERLLRAPVTGITRNTREIGDIVKQGDVVSFVDDEPVKSNIDGVIRGLIADN
                     TYVQKGLKIGDIDPRGIREYCFTISDKSRSVAGGVLEAILTLRKEKYGR"
     gene            complement(30361..30951)
                     /locus_tag="PKU88_07280"
     CDS             complement(30361..30951)
                     /locus_tag="PKU88_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPX69114.1"
                     /translation="MISGIILASGLSRRMGTDKLLLPVGGIPVIERVIAAASKSELGE
                     IILVCGSDSVADIGKKYRAKIVENNAPETGQSHSVGLGVKNSCRSAEGFMFIAGDQPF
                     LDESIINKLIGKFLPGHCNAVVPLYNGIKGNPVIFASKLRNKLMSLSGDSGGRVLLEE
                     MKEGIITVNFADAKPGLDIDTWEEYEAITKLEGENG"
     gene            complement(30956..31690)
                     /gene="yqeC"
                     /locus_tag="PKU88_07285"
     CDS             complement(30956..31690)
                     /gene="yqeC"
                     /locus_tag="PKU88_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HPX69115.1"
                     /translation="MESLYSQVNISIWKKEMICLVGAGGKTSAMFRLAKELSGTGKKV
                     LVTTTTAIYYPLENQNVQTVIADLLLPDLFKSIRSGITVYGRALSREGKLLGVDPEFL
                     DTLFLKDIFDYIIVEGDGSRGRPLKAPAEHEPVIPSLASRVLGLVGLDCVGKKVCPEN
                     VHRTEIFCNITGCREGDIIDADIIGRLIAHEEGLFKAVPSRAERYVVLNKADGEKEWS
                     AAGKIIQRLSDMGYEPDGIVVTKLGM"
     gene            complement(31967..33622)
                     /locus_tag="PKU88_07290"
     CDS             complement(31967..33622)
                     /locus_tag="PKU88_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HPX69116.1"
                     /translation="MKGNILSNNGQLAADEVLQAAGGGIPAAYGAKEPFIADSRDTIF
                     AVLAFVLGFFFVRWVMFSWQGWGVAVFTFGYYIAVTMYFIKKGVTITRSGFFWLAVVV
                     LVGISYSLYSNNGLEPWRSLFLFCGAVYYVISASGRLILANTSNWLILDGINAMFVIP
                     FKNFGCQYKSLYSSIHIKRSFGKQFLSIALGLMLTLLIAGMVLPLLLRADSGGFSRIA
                     NGLIEYFRWMQNQFSETIFNIILSIPVAAYLFGLVAGCAHNRGCNTFKREGIQYGVQS
                     MRVLAAATVYTVLGLICCLYMVFIGSQLPYFFSAFAGQRPEGWQIYSEYARSGFFELC
                     QIAAINLTLLTLANLFCKKPQKNYILKTLNCLLSLLTLLLIAAAFSKMALYIGAYGLS
                     IRRLLPCLFMVFLAVVFVGVIVLQKRQFSIVRLSAFTGTLIMCALCLLNPDGFVASYN
                     ADRYLAGTLKSFDVQILYQSGPAGIDPALKVYSRTADEELKAELTEYIYHQQRESAKK
                     LGRTQDNLQSMLVRQKTAEFAELISGDSFKSYVPGTEQKNVLR"
     gene            complement(33646..>33737)
                     /locus_tag="PKU88_07295"
     CDS             complement(33646..>33737)
                     /locus_tag="PKU88_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX69117.1"
                     /translation="KAKAIRFSTLEAICKALECQPGDILEFRE"
BASE COUNT         8149 a         9134 c         6479 g         9975 t
ORIGIN      
        1 cggcaataca tattcaaacg gatttatcat atttattccc tccttaattc attaaccaat
       61 tattcctttc cgttccagca gtacgtacag accttatcct tgctgattcc gattgcttca
      121 atcatatcgt ggatgttctg gtacttcagc gaagaaaagt tgagcttcct gcagattaga
      181 tccaccatat tatgatactt gtcggttgcc tgattgcaat actcctccag agaatccggt
      241 tccttgcctt caagttcttt gatagcctgt cttgccgcca ggtccatttc agaccgggag
      301 gccgaaaagt tcaggtactt gcatccgaaa acaataggcg ggcaagccga ccggatatgg
      361 acctccttgg cgctgcattt gtacagcagg tctaccgttt ctctcaactg tgtgccccgg
      421 acaattgaat catcacagaa aagcaatcgt ttgtcagcca caatttcagg aatgggcata
      481 agcttcattc ttgcaacaag gtttcttata tcctgatcct ggggcataaa gctccttgac
      541 caggttgggg tatactttat aaaaggcctt ccgtagggta ccttggattg gtttgagtag
      601 ccgatggcat gtccgaggcc cgaatctggt attccggcaa ctatatcaat tcccaaatcc
      661 tcatttttag ccattgccgc accgtttctg tatcgcatga cctcaacatt cattccctca
      721 tatgttgaag aaggataacc gtaataaacc caaagaaaag cacatatttt cattttatct
      781 ccgggaggtg aaacctgttc aaccacctcc ggtgtcagaa aaactatttc tcccgggccg
      841 agttcataat cagacttata tcccagattt gcaaaggtgc aggattcaga agaaatacaa
      901 taggcgccct ctttgctgcc gaccattaaa ggagttctgc caagcctgtc cctggaggca
      961 taaatccctt taggcgttaa aagcaaaaca gaacaggagc cttgaatcaa ttcctgagcc
     1021 cttaataagc cctctttaaa ggaatcttca tgatcaatga ttacagacac aagctccgta
     1081 ggacttatta tccctttatt cgtttccaga aaatgaatat ttcggtttga gaatgctttt
     1141 tccgcaattt catccaaatt gttgattctt cctacagtac ttatggcata ctgtcccaga
     1201 tgcgatctca ctgtcaaagg ctgagggtcg gtatcgctta tgcagcctat acccatgttg
     1261 ccgctttttt ttgacagttc agcctcaaat tttgctctaa actgcgtact ttctatattg
     1321 tgaatcgatc tgttgaagca gcttccgccc catattgcca ttcctcccct gcaggttccc
     1381 aaatgtgaat ggtaatcggt gccgaaatac acatccatca cgcaatcttc tcttgaaaca
     1441 acaccaaata atccgcccat tgcagctgca ctccctttaa catattttac cgcatatact
     1501 gttagatttg tcggaaaaga ccggtatata cattaatata gttgcatttt tattataatt
     1561 ttgcaagaat attttatttc caaaaaataa acagcacccc attcgtctat agaataaggt
     1621 gctgtatatg taagttgttc ctttcagcgt tcttccctga aatatggaat cccgcatgcc
     1681 ttcgggcctg ccttttccct tgacttccgc atggatacca ctacaagcac ggtaattgtc
     1741 accaggtacg gcagcatgtc tatgaaatac tgggatacat cgatacccag gttttgtatt
     1801 ctgaaaccga tgatatccaa tccgccgaac agataagccc caaagagcgc cttataagga
     1861 ttccatgttg caaatatgac caatgcaacg gcgatccagc ctctgcctgc tgtaacgccc
     1921 tcctgcaggg cgggtacata aacaagtgaa aggtaagctc ctcccaggcc gcagagcgct
     1981 ccgcccaata aagtatgcag gtacttgtac aaggttacat ttattcctgc agcatctgct
     2041 gctcccgggt tctcacccac cgccctcagg ttcatccctg tactggtctt atacaggtac
     2101 agccccagca gtatagatgc cgcatagcca aggtatacaa aggcatcctg cctgaagagc
     2161 acaggcccta taaaaggaat atcgccaagc agcgggatgg tgtaaggcct gaaaaacaat
     2221 ctcaagcttt caggagctat ctgacctatc agatcctgac ccacaaaatc cgcaaagcct
     2281 gtgccgaata tcgtaagtgt caagccggat acaacctgat ttgccctcag ggtgattgtc
     2341 aggaaagcga atatcaacgc gccgcccgct cccgcaatca ttgctgccaa taaagccaag
     2401 gtcgtgcttt cggtgtttag gccgaccatg aagccgataa cggcacccat aagcatcatg
     2461 ccttcaactc caaggttcag atttcccgaa cgctctgtta tgatttctcc cagcgtagca
     2521 aataaaaggg gtgtacctgc gacaatggcc gcatggagaa aggatataac agcttccata
     2581 atcaatcagc ccccttttcc gcaatagccg aatgcttttt tacaattacg aacttataac
     2641 gtacaaagaa ttcactgccc aatacaaaga ataaaattat tccctgaagt atttctgccg
     2701 ctgcctgggg tatgccgaaa acagtctgta tatatgagcc cccctccaga agagcagcaa
     2761 aaagaatgga tactatcaat ataagcggtg cacttaaagc tgccagccaa gcggttatta
     2821 ttgcggtata acctacacct ccggtaattt caactgacaa tgtgtagtta acagccgaag
     2881 cctggatcat accgacaagg ccgcagaggc cgccgctcag cattatggct atcaatatcg
     2941 tcttcttgat attaatgcct gcataacgtg ccgtattcat gctctcaccg attacggcaa
     3001 tttcataacc cttttttgta tggtttatga atatatagac aacgaccacc agtataagcg
     3061 caatcaccca gcccacatgt acccccagca gcttgggaag aaccgcgttt tggggaaaat
     3121 tggctatttt ggggaatccc agggactttg gatccctcca aggcccgtac tgtaagaaca
     3181 tgacccattt aagcgcgata tagttcatca ttaaagtaac tattgtttca ttagtccccc
     3241 actgcgcttt aaaaaatgcg ggtataagcg cccacagccc tcctgccagc attcctgcaa
     3301 gtatcattac cgtaagcatc agaggcattg gcatatcatt catgtataat gcaaaataac
     3361 tggctgcgaa agaacctatt gctatctgcc cttccccgcc tatattccag aactgcattt
     3421 taaaagctat cgcaattcca agggaggtaa ttaccagagg tatcgctttt actattgttt
     3481 cttttatcct gtaagctgta ccgaatgcgc cctgaatcat ttttatataa acatcggcgg
     3541 gattaaggct cagcagcagt ataaaaagtg aagaggccac gagagccagg ataatagcgc
     3601 ctattctgat ggaagcagct ttcatcctgc cgggctcgct tttcttaatg gctcgtatag
     3661 ttattggcat tatcatgctc gctcttttcc ccctctttca tcagatttac cgtccctgtc
     3721 atcatcaggc cgatttgttc ttttgtcgta tatcttgcat caactattcc ggttatttca
     3781 ccggcacata tgaccatgat cctgtcgcaa agctccagta agacatccag gtcttcccct
     3841 atatacagaa ttgcgacacc cttccgcttt tcctcattga gaaggtcata tatggtatag
     3901 caggagttta catcaagccc ccttacagga tatgcagtta tcagaacctg cggccgggag
     3961 tccagctctc ttcccaataa taccttttgt atatttcctc ctgagagcat gcgcaccgga
     4021 tgcttgattc ccggagtctg aatactgagc ctttctataa tctccacgga cttttgttct
     4081 gccggctttc tgtctatgaa gatgcccttc tgtttctgat acagcttaag cacgacatta
     4141 tttacgatat ccattgaggc aaccagtccc attcccaatc ggtcctcggg aataaagctc
     4201 aggctgaccc ccttggttat tatctctgca ggtgatttgc ctaccaggtt ttctccattg
     4261 aaaacaatct ccccctgctt cacgggatgc aagcctgcaa tagcttcaca caattccttc
     4321 tgcccgctgc cggcaattcc cgcaacaccc agaatctcac cggagtatat atcgaagctc
     4381 atccctttaa gcactttgat gccttctgaa tttacagcgc ttaaattcct tacttccata
     4441 gccacattgt ttttctcagt ttctatcctc tttatggaca ggtcggcagg acatcccatc
     4501 atcagctcgg taaggtgctt ggggttggta tccgccttgt caaccgtcat tactgtttca
     4561 cccttgcgca ggacagttat gcggtctgct acctccataa cctcgttcaa tttgtgagtt
     4621 ataaagatta cggcgcagcc ttcttccttc atcttcctca taatcttgaa aagcttttct
     4681 gtttcctgag gtgtcaatac cgccgtgggt tcatccaata tcagtatctc agcacctctg
     4741 tacagcacct tcagtatttc cagggtctgc ttttcaccca ccgacatgtt gtaaaccttt
     4801 ttgtccgggt caatatccag gccgaagcgg gccgacatat ccctgatctt gtctgaaaga
     4861 gtcttttcgt tcatcagaaa actgttcttt cgccccagga ttatgttctc tttggcagtc
     4921 atgacatcta tcagcttgaa atgctggtag atcattccta ttcctgcttc tatcgcatcc
     4981 tttggtgctg caaactgcac ctctttcccc ttaataaaaa tagaaccgct gtcgggggta
     5041 taaacccctg acagcatgtt catcaatgtg ctcttacctg aaccgttctc tcccaacagg
     5101 gcatgtatct cccccctgcg gacggccagg tttatatttc tgtttgcaac tacctttccg
     5161 aaggatttat ttatattctt cattgaaata tagttgttat ccataattat ttcctatcct
     5221 gacttattta atcttgcctt ctacaccctg tacaaaccag tcaacgctca ttatttcatc
     5281 aaaagtcatt gacttgcctt ccggtaccct gacagcgcca gtctgatcct ttatcgggcc
     5341 ctggaataca tagaatgaac catcgataat ctttgctttc accttgtcaa cagcttcctg
     5401 agtgcccgga gcaacgttat ccgtaagagg tgccaggtca acaagcccgt ctttcatatt
     5461 gccgtagtag ttttcctgct tccatttgcc gtccaatatc ctctgtactt ctgccgcata
     5521 gaacttaccc cagttccata cgggagcagt caggtatgct ttcggtgctg aatcccttgt
     5581 atccaggtca tagccgatag cccaaacgcc tctttcttca gctgcctgct gggttgctgt
     5641 tgtatcctga tgctgtgcaa gtacgtcgca gcctgcatcc aataaagcct ttgccgcttc
     5701 cttttccttt gcggggtcat accaggtatg tgtccagtta acctttacct ttacttttgg
     5761 attgactgac tgtgcaccaa gagtaaatgc attgatgcct cttacaacct cgggaatttc
     5821 aaaggctgct acataaccaa gggtattgtt ctgtgtcctg aggcccgcaa ctatacccgc
     5881 aaggtatctt gcctcttcca tggcaccgaa ataattgctg aaattcgggc cactcttata
     5941 gccggagcag tgatagagtt ttatgtccgg atgcgcctgt gcggcttttt ctgtatagtc
     6001 catgtgtccg aaagagttgg tgaatattac agtacaaccc tggtcaacca aatcattgat
     6061 taccttttcg cattcgctgg attccggaac atcttccttg tacattgtaa taaccttgtc
     6121 tccaagctgc tgttcaaggt aaagcctgcc ttcatcatga gcctgggtat atccgccgtc
     6181 tttagcggag cctatgtaga caaaacctac cttaaggggt tcagcctttg gctcttctgc
     6241 cggtgcaggt gtctcgggcg ctgccggtgc gggttcggcc ttcggtgcgc agcctgtcag
     6301 cattgctgca aacaccagga caagcactaa aagaatacta atggttcttt tcatactgat
     6361 ttctcctccc caaatttaat ttagtggtct atgtaaaatc atgaaactgg tggaatcata
     6421 attttgcttt tgttaaccgt gctaatcaaa aatgatgact ccttcgtcca tggacttaat
     6481 aattaccaag gattcaatac ggactccttt ttgccgcagc gtctccctgc ctttttggaa
     6541 acctttttct ataacaattc cgatgcccgc aaccctggca ccagcctgaa ggacaatgtc
     6601 caccaagcct gaagcggctt tgccgttagc caaaaaatca tcaatgataa gaacattgtc
     6661 ctccggagtg atgtattttt tggaaacgcg gatgttgtag ttcctctgct tggtaaaaga
     6721 gtatacttcg ctttggtaag tatccgaatc aagattcaat gcttcatact tttttgcaaa
     6781 aaccacaggc actttaaaat actgggcagc tatgcacgca acagcaatac ccgatgcctc
     6841 cactgtcaat atcctgctta tcttttcatc gctgaatctt tctttaaatt cccgtcctat
     6901 ctcgttgaac agctctatat ccagctgatg gttcagaaag ctgtcgactt taataatgct
     6961 gtcgcctatg acttttccgt ctttcaatat tctatcctta agcagctcca ttattatccc
     7021 tttctatcac ggctgtttct cgataatgta atagcatatt acctgaatcg gtgcaaaata
     7081 ccagtacatt acacattgta ttttaccgta aatgcctgtt taatgcaata agctattgct
     7141 taacaatcaa catccagagc ccttttcact gcattgaata ttttgttgta acctgtgcag
     7201 cggcataaat gacctgaaat ctcacgtttt atctcctcat cggaatattt ccttccgctt
     7261 tccgtaagag cagttgccgt aagcacaagg ccgggagtgc aaaaaccgca ctgcacggca
     7321 ccctcgtcta caaaagcctg ctgcactttc gacaattcac cctccttgga tactccttca
     7381 atagtagtta ttttcctgcc gtcagcccat accgccaggt aaatgcacga attcaccgga
     7441 aacccgtcaa tcagcactgt acatgctccg cattcgccta cggagcagcc ctgcttgact
     7501 ccggtaagat gaagcctgtt tctcagcacc tcaagcaacg attctctaat atcaacctca
     7561 agggaatatg ccttaccgtt aaccaccata cttatctttt catacttatc caatatccac
     7621 acccccgccc ctaatcacag cttctttcag tgccctgaat accaatacct caatcaaatg
     7681 ctccctgtaa gcctttgaag ccctccagga gtcccttgct cttgatgcct ttaccgcata
     7741 tcttccggtt tccttcatgg ttttatcgga tatttccttc ccaatggcat aagcttcagc
     7801 ttcactgcac cttatcggta caggggccgc aactccgaga gctatcctta catcagaaaa
     7861 cttacccccc tcagttttgc atacgacaga tacccccagc attgctatat ccattgcctt
     7921 tctgccgctg tattttatat agcagccgct ggtccggcca tatcctgcct cgggtatcaa
     7981 aatgtcggta agtatttcag caggaagcag attcactttt cccggccccg cataaaactc
     8041 gcttactggt actgttcttg acccgctgcg gcttaaaagc ttcagttccg cattataggc
     8101 aagcagcgta gccgcactgt ctgccgacac agcaccattg catatattcc cccctatcgt
     8161 agccatattt cttatctgcg ggcctcctac cgatactgcc gcttccgaca aaaccggcaa
     8221 attctcgttt attatgctgc tcctggctat tttggcaaag gccgccattg ccccgatgga
     8281 aatggttccg ttgtcaagaa ttcttatttc atccatcccg gggatatttc tcaggcttag
     8341 aagctcagct tcctcaagag caccgttacg catcttgatc agtacatccg tcccccctgc
     8401 tatgattttc agttcaggat ttcgggaaag catttccaaa gctgcctcca gagtgccggg
     8461 tttttcaata ttgacaatat caaacattcc tatctctcct ttctaaagca gccctgattg
     8521 tttaaacttt tcgaaaacct tctggggcgt cataggcagc ttgttgaatg caatgcctgt
     8581 ggcattcagt actgcattcc ttactgcagg tgcgggagaa atcgttgtat tctcacccag
     8641 ggatttttgc cctaccgagc cttcgggttc gtgcaattgg acaaatgcag acccgatatc
     8701 cggtgtatcc attattgtct ggagtttgta gtccaggaaa ttgttattaa ggggcctgcc
     8761 tgtttccggg tcgaacagca gctgctcgga caatgcatat ccaaggctca tgctgacacc
     8821 tccgtggacc tggccttccg caagcatcgg gttgactata actcccgagt catgcacatt
     8881 atatatctcg attacctcaa tccttcccgt ctccatatct gcttcaacct cggcaaaagt
     8941 aactccgtat gccattgcat tggttctcac attgacagaa gcatttccgc aaataggttc
     9001 tgcatttaat ctgtcataat aggaccccat ggctatgtct tcaagtgtgc agatttcatt
     9061 gccagacttt ctttcaacta tccttgccgc cttaatatca agctcatcga tgcttaatcc
     9121 ggttttttcc gatgccatct ccagaacctt ggatttaact tcctccgccg ctttttttac
     9181 agccgccccc gatacaaagg tttggcgtga ggcgtaagcc ccaagatcaa aggggctgat
     9241 atccgtatcc tgctgagata ctatatgcac catatcatag ggcaaaccca gtacttccgc
     9301 cgctatctgg gacaaaacgg tatcgcttcc ctggcctatc tccgttgcgc ctacctgaag
     9361 ctgaaccgag ccgtcctggt tcatgacaat ccttgctcct gagatttcaa aaccctgggg
     9421 ataggttcct gaggcatagc tgaagcaagc catacccagc ccccttcttt ttgtgccctt
     9481 ctgttccttg tatttttcca gtttttcgtc ccatttgatg agctgttttc ccttttcaat
     9541 gcattcggga agtccgaagg ttctcacaac gatttcagaa tgaggatctg tctttccggc
     9601 acttatcagg ttccgcttcc tgaattcgat gggatccatt ttcatttccc gggctatatc
     9661 ttctatatgg ctctccagag caaagaatat ctgcggacaa ccgtatcctc tcattgctcc
     9721 ggctgccgga agatttgtat aaactgtttt cggctcatac ttcattgaat taaagtcata
     9781 aaggagcctg gacttaccgc ctgcactcat tgcaatagta tggccgtggg aagcataagc
     9841 cccgttgttg cttatgggcc tcatatggag gcttaacagc ttcccttctt ttgtcactgc
     9901 tgtcttcatg tagaatttca ttgcatggcg ggttctcgta tcaacaaagc attcctccct
     9961 cgtgagtgca tagcgtacag gccttccatt gactgcaagg gacatggcgg ctgcaatagg
    10021 ttctactata atatcctgct tgttcccaaa gcctcctcct atgtatggtt tgataattct
    10081 gacctttccc cagggcatgc caagagcttg agcgacaatc ctcctggcta tatgcggaat
    10141 ctgtgttgaa gaaacaatga ctatccttcc gtcagcatcc gcataggcat atgcgctttg
    10201 aatctcaaga tggcagtgct gtacaatact tgtttcataa accccttcaa atatatggtc
    10261 agcttttccg aaatcctctt caatatctcc tatctcttcg cttgcatccc ccaaaatatt
    10321 atccggcttt tcctcatgaa tcaccggtgc gccgggcagc attgccttct cagggtcaag
    10381 tacaaaggga agtacctcat actccacctc aatcagctta agagcctttt ccgctgtaaa
    10441 tgcatcggaa gctacaacag ccgcaatacc gtcacctaca tacctggctt tgtctgtaag
    10501 tatcaacctg tccgccgcat ccctgtgatc cggttccaag gaccagggat ggcctgccgt
    10561 tgcatatttc agcttaggca gatcttcata ggtaaataca gcctcaaccc cgggcaaagc
    10621 ctttgccctg cttgtattga tacgttttac aattgcatgg gcataagggc ttctcaatac
    10681 cttccccaca agcatatccc cttcgaaaaa gtcatcggca tatttcgccc tgcctgtcac
    10741 tttggcaaac gcatcttttc taatcgttct tttccccact atttcgaaag ccatttttcc
    10801 accttcaatc atacagattt ttcatgattt aaaagcgctt ccatacttcc cttgccaatt
    10861 cacggcttct tgccatcatt ctctcttcat caatatttac cagttttctg tcttccatca
    10921 gaattctgcc gttcacaatc gttgtatcaa caaaacgccc gtttattcca aacaacagat
    10981 ggccgtttat attgccttcg tttataggtg taggcggatt ataatcaact attatcacat
    11041 cagccattgc tccggctttc aggattccca cctgtccatc aataaacctc tcaactattt
    11101 tcctgttgtt tgcaaacagc attgcgggaa tctcacccca ggcaacattt gaatcccctt
    11161 ttacatgctt atgaattata tttcctacct tgtaggattc catcatatcc gctgtatatc
    11221 cgtcagtccc caaaccaaga agaattccct tgccgaacat ctccggcacc ggtgaagcac
    11281 ctaccgcatt ccccatattg gattcgggat tatggaccac tattgtgtcc ttttccttca
    11341 gcatatccat ttctttttcc gatatgtgga tgcagtgcac agctatggtc ttactcccca
    11401 gtacccctga atcgtaccac ctttcgataa cccctttccc atatcttgcc ctcgaatcct
    11461 ccaaatcctc aattccctcg gcacagtgta cgtgaaaacc cgtatcaagg ccgcttatgg
    11521 catccaggca tttgtccagt gttttttccg aaatggtcat ggatgcatga aggccgaaca
    11581 ttcctttaag catatcatcc tttttattgc tgcagtactt tataaattcc acattttccg
    11641 caataccttc ttcggcaatc ttctcaccgt cacggtctga ggtttcgtag cacaggttgc
    11701 ttcggatgcc tatgtgttct gccgcctctg ctattttgaa caggcttcct tttacggcat
    11761 aagggcttgc atgatggtca ataactgtgg tcactccgca ttttacctgt tcaatcatcg
    11821 ggcccacggc gctgtaatac acatcctcaa gggtcagtac cttgtcaagc ctccaccaca
    11881 agcccttcaa tatctcggag aactttgtgg ccggcgggct gtcgttggac atacccctgg
    11941 cgaaggtact gtaataatgc atgtgggtgt taataaaacc cggcattatt agcttgcctt
    12001 tggcatcaat gaaccttgcc ccgctgtatt tctccttaag ctccgaggtc agccccaatt
    12061 ccgctatctt attgccgtct atggcaatac atccgttttc gatgattgga atgtcatcat
    12121 ctcttgtgat tactctgccg tttcctacta atatcatttt tccccctcct tattcagtgg
    12181 tttcttatgc tgtttctttg cctcttcact atttcaacag ctgttttatg cgcatctgat
    12241 gccacccggt cagaatcaat tgtcgtcagc tttccgtcct taactacgac tctgccgttg
    12301 acaacagtca tgtcaaccat actggaattc ccaaggcata tgagagatac cagcgggtca
    12361 tggcagccgg agtattcgat gcctttcagg ttgaacagca ctatatccgc cgctttacct
    12421 atatccaggc atcctgtgtc atcccgtccc aacacctctg caccgcccct tgtagccgtt
    12481 ctgagtactt ggtacgctgt aagcccgtca gtgccgtatt tcaggtggtt aagaaggtaa
    12541 gcccttctta cttcctccca catatttgaa ccgtcattgc ttgcgcttcc gtctacagct
    12601 atggaaagct tccctcctgc acggattatt tccgttgtcc tgcatatgcc gcttgagagt
    12661 ttcatatttg aggaggggca gtgggctatg cctgagccct gaagcctttt tatttctcca
    12721 tcattcaaat aaatgccatg ggcgaaccat acatcatccc ccacccattc caggtcctcc
    12781 atcagctcaa aaggcctcct gccgtaacgc tccaggcaga atttctcctc gtcaagtgtc
    12841 tctgccagat gtgtatgaag catgaccccc ttctcccttg ccagttcctt cgtctgcacc
    12901 ataagctccc tggtcaccga aaagggggaa caaggtgcca aggctatcct ctgcattgaa
    12961 taagggctgc tgtcatgata tttgtttata agcctttcgc tgtcggcaag tattgcttcc
    13021 tcagcttgta caacctccat gggaggcagc ccgcccatat ccctgcccag ggccatggag
    13081 ccccttgtgg catggaacct tatgccgatt tcccttgccg cccttatctg gtcatcaatg
    13141 agtgttcccg gctggttctt cgggaataca tagtgatgat cggaggtcag agtgcagccg
    13201 gtgcgcaata actctgcaaa tcccaccagc gctccgtaat aaacagcttc cggggttatg
    13261 ttcttccaga actcatataa tcccaccagc cacgggaata ggggcttctc ctgtacttcc
    13321 tttatacctc tgaacaaggt ctgatagaga tgatggtggg tattgacaaa gcccggcagt
    13381 gcggttaatc cgctgcagtc tatcacttca gcatctcccg cagtgtcaag tccgctgcct
    13441 atctccctga ttgcagtccc gtcaatcagt atgtcgcagt gttcaaaggt ctcctccctg
    13501 tcgttgaagg tgactatcag gtcaatgttt ttcaaaagct tctgactcat atttcaagtc
    13561 ccccaaataa cttatttgta ccggtattta agaataatgc ggcgtgtctg aagttcgcat
    13621 tcagccttca gagcactagc cttgtatatg aaggatatgt aaatgccgac gtggggaagc
    13681 tatattcgac gaaactttcc accgacctcg gaacgggaag gcttccaagc aaaataacca
    13741 gacggactcg cagagatggg acccgtgcca gtgattttgc cggctttgga aagtgaggag
    13801 aacatacttc ccctacagtc ggagtacttt gcaaaccctt gattttaagt cgcattcttc
    13861 ttatgtgcta tctacagcat aaatgaatac ttaccgatgc aggcctttat gattgcagcc
    13921 atctctttgg acacaccatt ttcctgccct aatgcatagt ttactatttg accttcctct
    13981 gtccgtacaa ggcatttgca cgctgccata tcggttacca ggaagccctt attgttgctg
    14041 tccctgaaat cttcctcatt ccagaaaacc gtaaccttat ccttgtacgg gtttccttca
    14101 tgggggcaga atattccgca gttgccgcac tcgttgcaca taccgtccag atgtataacc
    14161 tgatgcccgg aggcaaagcc ttcgtcaaca aatatcactg catttgccct gtttggacac
    14221 acatcaacac acaactcgca tatattttcg caggagaggc atctttcagc ttccttatca
    14281 ctatatacgg gattattcaa tatccctttc ctttcataaa gaatcttctc atccacgggt
    14341 aataccttct tttcaaaatc agccttcaag ccttccttgc tcaatatatc ctttgcagcc
    14401 gcctttccgt cggctattgc tctgacaatg gttgcgggcc cggctttagc atctccggcc
    14461 acatataccc cgggtatatt tgtctcacag gcagagctga cctttggaaa accttttgaa
    14521 tccagttcta ttccgttttt tgcaagaaat atgctgtcaa cctgttcacc tacggcaaca
    14581 attaccgtat cggcatcaag ggtaattttc tctcctgttg gcacggggct tctcctgccg
    14641 gaagaatccc tgtcccccag agccattact tcacatacaa gctttccttc tttatatgct
    14701 gccggcgaaa gcagttccct gaataatact ccttcggaag cagcaagatg tatttcctca
    14761 cggtctgccg gcatgagcgc ctttgtcctc ctgtatacta tggataccga ttccactccg
    14821 ggaaccctct ttgctgccct tgccgcatcc atagccacat ttcctccgcc tatgacacat
    14881 acatggcttc ctaatttcat atttcccttt tgtgctttgt aattctccag gaaggatatt
    14941 gcatttaccg tcttttccgc tccctcctca agggatatct gtccgggctt acaagcgcct
    15001 attgccagca caacgtaatc atattccttc ttcaaagctt cgacgctgaa gttttcatta
    15061 atgccaagct tgaatcccac tccgctcctt ttgacaagct caagatcctt tctgaccata
    15121 tcagaggata tcctgaactc aggaatcaca tattcaacta caccgtaagg cctttccctc
    15181 ttatccatta caacagcttc cattccgttt ctccttagga acagagcagt tgcaagtccc
    15241 gcagcccctg cacccactat tgccgctttt ttattgcttc tgatttctgc cggcctgata
    15301 ccggcggtat acttatcctg cgccttgagg gctgctgttt tcttcatttc cctgataaga
    15361 accgattcgt cataatccaa gcgggtacac ttgtactggc attgatggtc gcagattgag
    15421 cctgtgattg ccggagaagc attatcgctt actattactt caaaggcttc atcatatttg
    15481 ccttctccca ccagagcaac atactgaggt atctgctggt taatggggca gcccattgca
    15541 cagggagata cggcacagtc atataaagga agctctgagg tcagtttcct gctgttaacg
    15601 ggtctcaact ctttaaggtg atgcttatct gttaacgcgt tctcagcaag gctgcttatt
    15661 tttgcgatgt caatacccgc agccattctc ttctgcagcc cttccgcctt gtggcccaac
    15721 tggtttatcc tttcatagcc tcccggcttc agtatcgtgg ttgcaaaggt tataggcctt
    15781 actccggctt ccattacctt gtcgatatta aagaaatcaa ttccgccgga ataggagata
    15841 tgcaaatctc cgttgaattc ctttgccagc ttgcttgcaa gagaaattga aagggcatac
    15901 agtgatcttc ccgacatata catttcttct cccggcagct cctggttgag aatcttaacc
    15961 ggcagtgtat ttgtcaattt tacccctatc tccagattta gttctcttgc aagggctttt
    16021 aggcgcttca gcattgccac ggcatccggg aactgcaaat catgaatgaa gtggtgcccg
    16081 ttcagggtta tatacccata tcccattgca tccagggtat ccctgacaaa cttttcgccg
    16141 agaagtgtcg ggttcatctt tacaaaagta tgcagcttct tttccttcag aaaataattt
    16201 gctattcttt cgatttcctg gggcgggcat ccatgcagcg tagaaagtgt aatggaaggg
    16261 cagaccacag gagaaatcat atccaggtct tcccttgtga aatgagtaaa ttgattcatg
    16321 gcggaaagca gtactttcct gcattccttc catatatccg tattggctgc attgcgcatc
    16381 ccttctatat atgcgtccat tttaggggac ttaatgccct ccaggtcata gcctacactc
    16441 atgttgaaca tgaagtccct tttttcgctt aatccaagct ccttcataag aacatgcagc
    16501 aagaaccatg ccttgatata ctcattatat gcatcaggaa cccttaattc agtagaccat
    16561 tctacgttgt agcattcatc ctgggcatat atgcatggtt tggatacggg caaatcttct
    16621 ccgtcaagct tttgtaccgt tttcaactca atgaaacggc agcctgtcaa atacgcagcc
    16681 acaatattct gcgccagctg cgaattgggt cctgctgcag ggcccaaagg ggtcgccagc
    16741 ttttctccaa acaactcggt gaagcttccg cttttatttt tgtaaaattt gcttttgtgg
    16801 ataccgaaaa ttgagtcctt ttcctttaat tcggacaaca cccaggaaag catcttctca
    16861 aaaggtatca gcctcattct atcactcatt cttctcctcc tattcaagta ccatatatga
    16921 tctcaatggc acagtatgtt aatcagccag attctcagtg gttttcggca ttttatcagc
    16981 atactttttt acatagagcg agggaattgc cgcatacatg gcagcggctt tcaccaattc
    17041 gtccttccag gtcttttcat ttggggcatg ggcctgatct tcatgacccg gtccaaagcc
    17101 tatgcaaggt atgccgtatt tacccattat tgaaactccg tttgtggaga atgtccactt
    17161 gtccaatacc ggctcatcct cgaaaagtcc tttgtatgct tccagcaagg tactgcatac
    17221 gggatggtcc tcttctatga accaggtggg gaaatacgct tctgtgggat attccagccc
    17281 cgtataggag ggcctgctgt atggatacat tgtcacctca gctttagctg ctttgaccga
    17341 aggaagctcc ctgatctgat taagagcgta ctctccgctt tctcccgctg tcagccttct
    17401 gtctaccgaa atccagcagc tgtccgccac tgcacatctt gacggagatg tatggaatat
    17461 ttcagatact gtcagggttc ctttgcccag gaacggatgg ctgatgagcc tttcattcag
    17521 ctctttcaat tccatgagta taggagccat cttatatatg gcgttgtcgc ccctttccgg
    17581 agcagagccg tggcagctta caccctgggt tgccaccttg atttccattc tgcccctgtg
    17641 ccccctgtat atacggcagg aggtgggttc agtcaggacc acgaattccg ggcggatatt
    17701 catttcctta atgatatact gccagcataa gccgtcacaa tcctcttcct gtacagtacc
    17761 cgtaatcagc aatgtgaaat catcctccag cttcaggtcc ttgattatct taccggcgta
    17821 taccattgcg gccataccgc cttcctggtc gctggcacct ctgccaaaga ttatctcgtc
    17881 attctcatag cccttgtacg gatcgaactc ccaatttttg atatttccga cccctactgt
    17941 atctatatgg gcatccatgg ctatcaggtg tttgccgtgc cctacatagc ccattatgtt
    18001 gcccatggga tctaactcta ccttgtcaaa accgacagcc ctcatttcct cggcaatcct
    18061 accgataacc tctttttcac cgcagctttc gcttggaatt gctatcatat ccctcagaaa
    18121 tttggacatc tgcggtttgt atttctctgc aagctcaagt atttgtttaa aatcatacga
    18181 catttcatgc cctcctaata tcctaaaccg gttcttttgt tgaattcctc tatcaagctg
    18241 tcaagctcag gcgccatttt gtgcagcctt ccccagtagt tgtcatagtc gtaccactgt
    18301 tcattcagct catccaggtc atacatctgc tgctcgatcc aggtgtagta tttcaggtta
    18361 tgaatacgtt tcctgctctg gtaggtcagc tcttccatgg tatctgtacc cagtgccaat
    18421 acatacctgt tatggtctat agctgcatca acattggagt actttcttcc ccttgcttct
    18481 tccatctcca taagccttga ctggtacatc tctgctgaat ctgtgagtac tgtcattact
    18541 atatcatttt ctgcaagctc atagtattta gcaaacttga tacatgatat gatgtttgca
    18601 gcccctgaaa ttcctaccca ggacagcttt tctacaacct catcccttat gcccagggat
    18661 ttcagatatt cctgtcctgc cggttcattg aagaggcgga acaaacccaa actgtcatta
    18721 tcatctattg ctataaccat atcggtattc tttacattat gaatccacgg cacgtgcttg
    18781 tcacctattc cctctatcct gtggtctcca aagccgttac tgagtagtgt gggacactgc
    18841 agtgcttctc ctaccgcaag ttttgcattt gggtaccgat cctttatgaa atctcccgca
    18901 cccagcgttc ctgccgagcc tgaagtcagg catacccccg ccacttttcc ttttgaacct
    18961 gcctcagcat tgattatgtc ttccattgca tggcctgtta cctcatagtg ccataagtgg
    19021 ttgcccaact cgccgaactg gttgagtata acaacattgt ccctggtcct tctcaagtcc
    19081 caggtcttgt catatatttc ctttacattg ctttcgcagc ccggtgtggc aataacctca
    19141 ccggcaactg tcttgagcca ttcaaaacgt tctttgctca tgttctcagg cagtattgct
    19201 atggagctgc agccaagcag cgtcgagtta tatgctcccc ccctgcagta gttgcctgtg
    19261 gaaggccaga ctgcctgatg gtaggtaggg tcaaactgtc cggtgaccag tcggggaacc
    19321 aggcatgcga agcttgcacc taccttgtgt gcccctgtag gaaagaactt gcccgccata
    19381 gcaaatattc tggctttaac ccctgaaatc tcccttggaa tttctatata attaggcaat
    19441 ttattataca gtccgccgtg cttgacgggc tcgttcttcc aggatattct gaaaaggttg
    19501 ataggatcca catcccacaa gcctgtgttc ttcaattttt cctttacctt tgcgggtatc
    19561 ttgtcgggat ccttcatctg ggcaaaagtc ggaataacta tgtttctttc ctttacgttc
    19621 tgcaccgcct tttcaagccc tttttcgttg attgtaaaat caatgagctt tctcattttt
    19681 tcccctccta agcttattta caatttaaat ttactattac gggaacgcct taaatgtgca
    19741 caattcgttt cagatcattc aggtcaggtt ccacatggtt tgcttttccc gcagctttca
    19801 ctgcactctt tatgtccttc agcccgcttc cgctgactat gcttactacc ttttcatcac
    19861 cttttaaaag cccaagctct ttcatcttca taattcctgc aaagcttgtt accccggcag
    19921 gttctccgaa aacccccgtt ttttgcgcca gtattttcat tgctgcaagt atttcctcat
    19981 cggatacatc caccatgtac ccccctgatt cgtgcagggc gttcaaggct ttgatgccgt
    20041 tccggggtat ccctaccgaa atactgtccg ccaaggtatc aggcactctg tattcaaatt
    20101 cataagtttt cttattaaaa gcccttgtaa ccggatttga gttttccgcc tgaaccccta
    20161 ctattttggg gaacttgtca atgagtccaa gctcaaagca ctccttgaag cccttccata
    20221 cccctcctat ggtgcagccg tcccccacgg ccaccacagc tatatcggga gccttccagt
    20281 ccagctgttc gcagatttcg tatgagattg tcttcttgcc ttccaccagg tatgggttga
    20341 ttgcacagct tctgttgtac cagccgaatt cttcggcagc cttgaagcaa agctcaaccg
    20401 cttcatcata ggtgcctttt acaaggaata cattggagcc gtatatcaaa agctgtgtca
    20461 ccttagcctc cggagctgtt tccggcacaa atatataggt cttaatcccg gcaaccgctg
    20521 caaatcctga caaggaagaa gccgcatttc ccgttgaagc cgcgcacatg acattccttc
    20581 caagctccac agcctttgcc actcccactg cacttgccct gtcctttaac gaagctgtgg
    20641 gatttctgga gtcatccttg agaaaaaaac tgctcagtcc tgtttccttt gaaagggact
    20701 ttatattgta cagaggtgtc catcccactt gaaggggctg tattccgccg gttttctcca
    20761 aaggtattgc gggaagatag cgccacaggg aataatcctt gttgtttttc agatattccc
    20821 gggtcaattc cttcctgata ctgtcataat ccagttcaac gtccaggatt ccatctatgc
    20881 cgcaatcacg gcacgtataa agtatctcag cttcattgta ttctcttccg cacttgacgc
    20941 atttcagctt taataccttg ctcattcctc caccttcttt ctatcataag gggttccctc
    21001 cagggggagt tcaaaccttc tgatgccgtc aaacctttga taggctgaag ctacggcagc
    21061 agccaccgga atcagtgata tctctccaat cccttttgca ccgtaggcca gctctgattg
    21121 attcttttct atgattttta catctatttc aggtatttcg tccgccctca gcaagccaag
    21181 ggttccgaac ctggatttta aaattcctcc ttcatatatc agcctttccc tcaatgccga
    21241 gccaaggccc attactacgc ccccctcgat ctgtccttcg atatttaaag ggtttatcgc
    21301 cctgccaatg tcatgggccg ccactatttt tgcaatcctg ccttcggcat cgagtattgc
    21361 taaatgtgtg gcatagctgt atgccacatg gcttacagga ttttccctgc ttgaatttat
    21421 cggatcagta accccactat attcaccttc aaaaactaca ccttccaact cttccggagt
    21481 tttttccgcc aatgccgcct tcaacttgag tgcagcccgt cggcaggctt caccggtaaa
    21541 aagagtctgc cttgaggctg ttgttgctcc cgagtcggga cacaataagg aatccggctg
    21601 actgaaaatt atgtcagatc cgtcaatacc gagggtttca gacactatct gaatcattac
    21661 ggtagccagt ccctgcccta tgcaggaggc ggcagaatat acaacaacct tgccctcttt
    21721 tattcttata cggcagcgcc cgacatcagg caagcctact cctatccctg tattcttaaa
    21781 agcgcatgct attcctgcat atctgctccg gtagtattca tccctgactg caaggagtgt
    21841 ttccttgaca gctgtatcat cgccgcatac ctggccgttt gccagcacca tccccggttc
    21901 tacggcattt ctgtagcgaa actcccacct gtcgatgcct gccaggtctg ccagctgatc
    21961 catgttggcc tctcccgcaa aattggactg aggcactcca aagcctctga atgcgcctgc
    22021 cggggcattg ttggtatata cacagaagcc ctccagttcc acattgggga tactgtatgg
    22081 accgcagaca tgagtacatg cccgctccag aaccgcagtc cccaaagacg cataagcgcc
    22141 tgtgtcggct attatccttg cagtcatgaa ggtcagcatt ccccgttcat cacagcctgt
    22201 tgcaacctcc agttccatac cgtgccgttt tggatggcat aacaggcttt cttccctggt
    22261 aaaagtgtat ttgcatggct tctttgtata aaaggcggcc aaagctgcat aatgctgggc
    22321 gctcaagtcc tccttgcctc cgaagccgcc gcctacattt tgcagaatca ctcttacctt
    22381 gtcatgaggc aatcccagaa cacggcataa ctcatgatgg tcgtgggtga tgctttgggt
    22441 agcggcatac actgtcagaa catcgacctc ataaaaggct gccgcgcttt caggctcaat
    22501 aaaggcatgg tcaacgggag gcgtcctgta ggtgttcttc accacatact tggactctgc
    22561 cattgctttt tcgggatttc ccctttttac ataagtcctg cttaaaatat tgcccttctc
    22621 atgaattttc ggcgcatcgc tcctcatggc atattttgcg tcggtaacag gctccatttc
    22681 ttcataggtc acattaataa gccttattgc agcttccgcc agttctttag tttcagcggc
    22741 aaccgctgct acggcatctc ctgcatagcg ggtcatttct cccacaggta caaaggtggg
    22801 ccaatccttg aaaatatacc cctgataatt ttctcccggt atgtcctctg ctgtcaggac
    22861 agccttcacc ccttccaggg ctttggccgc gtctgtatcg acggacagca cccttgccct
    22921 gggcttcatt gccctcagaa cagaaacata aagcatatcc tccatgaaaa catcatcaca
    22981 gtaagttgct tcgcccagtg ccttctgtat ggcatctacc ctgcgccaat tctgtccgat
    23041 attggaactg catgtactca agctgatatg ctttgattcc cttaaagctt cggcagcaag
    23101 ccttattgcc ttgacaatct tcacatagcc tgtgcatctg cagatattat tcctgattgc
    23161 cttctttatt tcttcttcag aaggatccgg gtttttatca agcagcgcct ttgaagccaa
    23221 catcattccc ggggtacaga agccgcattg tacggccccc gcttcagcaa aagcatttga
    23281 atacacttct ttttcttttc cgtcaagccc ttcgacagta agcacttccc tgccctgcag
    23341 cttatcggcc ttcagtgtac atgaccttgc tgccttaccg tctatcaaaa ccgtacagct
    23401 gccgcatacc ccgctgtcac agccattttt aacagatgtc agcctttctt cctcccgaag
    23461 gtatttcaac agattggttc cttcccgtac atttactgcc cttccgttca ccttgaagct
    23521 gatcaagtgc atcacctgcc ttatttgctc tatttaacga aattaagtat catattttct
    23581 gctgcatcaa aataagcgtc cagattgaat ttaccttcat caataagata ctgcatagat
    23641 gctcccatca ttatggaaac taacaaatac ggaagcattg cacattgctc agatttacat
    23701 tccggaaaac ccggcatctg attcaatacc tctgttatgt tacttctcca tatattaaaa
    23761 gcgcctatga atttttccct tatttcaggg tttgcggttc cctgtaccca gaaatcaaac
    23821 tgcacataat caattttttt gtggttatat atgtcatcct tcttctcatg aaaaagcccc
    23881 cggagatttt cttcaaaaga ctttttctcc agatccactg aactcatcct tttctcggtg
    23941 aatctctcct ggattctgtc cagcaatgca atcatcaggt catttttggt agggtaataa
    24001 taatgcacgt tggattgtac cacccctgcc tcctttgcaa tcaaatgcat cctcgtgccg
    24061 cttattttat tctgcgcaac tatttcaaga gctgcgtcca gtattttgtt tgtaacttca
    24121 ttagacataa cagtacctcg attttcgtta aaaatatacc ttatctattt tttgccggtt
    24181 tatttcccgg agaacctggg gtaatttgaa tccatcagca tttcaaaggt ctcaggcaca
    24241 tttttgacct tgctcaggaa tatcatcgca gcaataatgt acggcttata accggcctgt
    24301 ttatataaag gtgtacgata ccggtcaaac acagatgcgg caacttcccc ttccttgcag
    24361 ctgactccgg taatatcagc aggcaggcag tgcaaataca gggctttacc gttctttgta
    24421 agcttcatca tttcttctgt gcattcccag tccttatgcc cggcgttctg ggcaaggagt
    24481 tccttttcaa gagctttgat ttcatcaaac tttccttctc catagagatt cgtcctcttc
    24541 tccattgcgg catagggagc ccagcttttg ggatatacca catcggcatt tgcaaaggct
    24601 tccttcattg aattggtctt ggaaaatttc cctcctgatt ttgcggcgtt tgctcttgct
    24661 atatcctcaa cgtctgacag cagttcatag ccttccgggt gagccaaagt cacatccatt
    24721 cccattcttg tcatcaggcc tattaccccc tggggcactg acaaaggctt gccgtaggaa
    24781 ggagaatatg cccatgtcat ggctatcttt ttgcccttaa ggttttccac accgccgaaa
    24841 tagtttatta agtgaagcat atcggccata cactgtgtag gatgatctat gtcgcactgc
    24901 agattcacca gggtcggcct ctgctccagg actccctctt catatcctgt ttgtacggct
    24961 ttggaaacct ccctcatata tgtattgccc ttgccgatat acatatcgtc gcggataccg
    25021 ataacatcag ccataaagga aatcatgttt gcagtctccc gcactgtttc cccatgagct
    25081 atctgggact tcccttcatc caaatcctgt acggtaaggc ccagcagatt gcatgcgctg
    25141 ctgaagctga acctggttct tgttgaattg tccctgaaca gagatactgc aagcccgctg
    25201 tcgaatatcc tgggagatat attgttctcc ctcattttgc ggagtatttc cgccgcctgg
    25261 aatactgcat gaatctcatc atcggttttt tcccatgtta aaagaaaatc attgttgcac
    25321 atagcattgc tgtcaagctt tttgaattgg ctaaccagct ttcttatttc ttccatctgc
    25381 atctccccta ctatctaata tctttataat ttccatatga acatattgag aaaaatttga
    25441 ttgtctagtc aatataatta tagaatgttt agtaaattct gtcaataaaa atgctaagca
    25501 tttttcgggt acgaggttcg aggttcgagg gaggacacgg ggacgtttcg aggacacggg
    25561 gacgtttctc ctgtcccgct aaaagcggga caggagaaac gtccccgtgt cctcgaaacg
    25621 tccccgtgtc ctcttctata atccttactt cctaaaacac ttcacaaatt tgccccatcc
    25681 gggctctccc aaaaaggagc cctcctcata caccagtctg ccccgggaga aggtcataac
    25741 gggatagccg ctcatttcac agtctttcca tatggtgtag tctacatcgg aatgcatgtt
    25801 ttctctagtc acaataaatt tcttttccgg atcatatata actatatcag catcgctgcc
    25861 cacagctaaa gttccctttt gcggagcgca gccgaaaatc cttgctgcat tggcggaaca
    25921 aacctctact gccttattga aggtaatgtt ccctttattt gcctcactta acatgtaggg
    25981 gtacatgttc tcaactccca tgcagccatt gggaatcttt gtgaaatcgt ccttgcccca
    26041 atccttttca taagactgga aagggcagtg gtctgtggca accgttgaaa tatatcctct
    26101 tctcaggcct tcccacaatg ccctccggct gtcctccccc ttaatggaag gagagcatac
    26161 gaaattcctg ccatcctctc tcttatacac ttcattgttg aaattcaggt actgggggca
    26221 ggtctccgca tatatttcaa agccttcgtc cctggctcct gaaacttcct ccaagccttc
    26281 cctgcttgcc agatgcacga tgtacagggg agcatcgaag gttctggcaa gatagaccat
    26341 tctccttacc gcatctgctt ctacgaactc gggccggctt tcataatgat accatgccga
    26401 tgttttcccc tgtgacagca gcttctttat tcttctgtca ataatgtatg ggttttccgc
    26461 atgtacggca accaaggcac ccgtctcctt tgaccttacc aaagcatcgg ctattacccc
    26521 gtcatccacc atcatatctt tgtaaaccat atacagcttg aagcttggca ctccgaactc
    26581 tacagaagcc ttgaactcat caaggacttt atcggtcagg tcggtaacgg caatatggaa
    26641 ggcataatca acacaagccc gggacgcaac atatcccagc ctgctgtctg ctgtttccac
    26701 aagacccatg cctttctcct gggtaacgaa gtcgaatacc atcgtcgtac ccccacaggc
    26761 agcagcccgt gtaccggatt cgtagctgtc cgccgagacg gtttctccga caggcagctc
    26821 cagatgcacg tgagcatcaa tggcacccgg cagtaccaat ttgccggcgg catcaacaac
    26881 cctttcacct gctgcctgta tattccccga aatcatcgca atcctgccgt ttttcacagc
    26941 aatatcagct ttatatgact ccgaagcagt aatgattgtc ccattcttga ttatcaagtc
    27001 catatcttca ctcataaaag gcacttcctt ttatccgttt attttgcaag cagctatata
    27061 tcaataaaac tgaagcttcc aacatcaaag aggcccctat ccgttaccct taactcgggg
    27121 atgaccggca gcgcaagaaa tgacagggtt acgaagggat caattccgtc aggtatttta
    27181 aggctttttg ctgactctat catgccggac agcttgtttt ccacaaaaaa tgaatctctg
    27241 tcactcataa ggccggctat gggcagcggc agcgtacctg ttacctctcc gccgcttaca
    27301 acggtataac ctccgttagc ttccttcagt actttcgcag ccaggagcat gtcagcatcg
    27361 ttatcgccga ttatgataag attatgggaa tcatgggcaa cagtggatgc tatggcacca
    27421 ttcctgatac caaagccttt tacaattcca agccctatgt ttcccgtggc tttgtgccgc
    27481 tccacaacag caatttttga aaacacctca tccggtatga aattgtttcc gttgtccttg
    27541 ggcactgcaa gcctttcttt ttttgtaatc agctgattcg ggatgatggt tataacattt
    27601 gccttatcac ctttaatttt tattttcagg tgttcagggg ataagttagg gattcttact
    27661 gtgtttaata catttgggtc atctatgatt ctgcttatat caggcttttt ccccttttca
    27721 caaacaaccg atccgcggaa aagcaccttt tcaatatcaa agctgtttaa atcggacaga
    27781 ataagcaaat cagccgaata tccgggggct atagctccta agcgctttaa atggtagcac
    27841 ttggccggat ttattgttgc catttttatt gcttcaatag ggtctacacc caggctgatt
    27901 gcctttttta cattgcagct tatatggcct tccctggaaa tatcctccag gtgcttgtcg
    27961 tcagtgcaaa acaggcagtg gttcagagga acgtcccgtt caataagccc tgatataatg
    28021 tcctcaaggt ttctggcggc tgagccctct cttatctgca catacatgcc aagcctcaga
    28081 tgtgcgatga cctcatcaat atttgtgcat tcatggttgg tcagcacacc aacagccttg
    28141 tatgcacaaa gctcatgctc cgataatccc gaggaatgtc cgtctacaac cgcattatca
    28201 taaagctcga ttttgttcag gatatccctg tccccggcca gcactcccgg gtaattcata
    28261 acctctccaa gcccgagtat atggcagctg cctgcaaagg gctccattac ccttgccgta
    28321 agagcggctc cgttgttttc aaaaggagtc gcaggtacac aggaaggcat catgtaaaat
    28381 atattcatag gcacgttgcg ggcctcgttc agcatgaaat gaatgccttc aataccgcat
    28441 acattggcaa tttcatgggg atctgcaata atcgtagtcg ttccatgggg taaaacaact
    28501 cttgcaaatt cagccggagt tatcatgctg gattcaatat gaacatggca gtcaataagc
    28561 cccgggcaaa catacatgcc gccggcatca atctcttccc tgcctgaata gtctccgaca
    28621 ccaactataa ggctgtcatg gattgcaata tcgccggata ttatttcatt tgtaaacaca
    28681 ttcacaatac tggcattttt gattgccagg tctgcttttt ccctgcccgc cgcaatctct
    28741 attctttttt gtttctgttc aacactcatc tcaaatccct catatgctta aggctgccgc
    28801 ctttttttac aagcattatc tcagctataa tactcaatgc tatctcctga tgggagtccc
    28861 ctccaaggcc caagccgata ggtgcataca cggcttccag gtcctccttt ttacagcctt
    28921 cactgagaag cctgttaaag acaaattgtg tcttctgtcg gctgcctatc atcccaatat
    28981 aacctgcgcc ttttcctatt gctgctctta gcgcatccgc atcgcttgca tgccctctcg
    29041 ttacaatgac tacatagcag tcttcatcta tgggatattt actcatgcac tctccaatat
    29101 ctcccacatg gatttcacag ccgggaaacc gttcattatt accatattcc tcccggtcct
    29161 ccattatgac aatatgaaat cccatcagct ttccaagctt atgcagttca agggctatgt
    29221 gccctcctcc cgcaataacc aggctgtctt ttgccttgaa tacctttata aagatatttg
    29281 ccttgccccc gcactgcata tggagttctc cggtatcatc aagctccatc tccattagct
    29341 tgttctttcc ttcactcata cagagaagag catattcccg tgctttgttt tcaagattgc
    29401 cgccccctat agtgccaaga gtgcttccat cagcaaatac agccatcata aagccctttt
    29461 tgcccggtga tgaaccctgc tcctcgacca gagtcacaag tgcagccttt ctgtttgcct
    29521 ttacttctgc ggcaagcttc tctaaaatcg catactccat aatctcacct tccatacttt
    29581 tcttttctga gtgttaatat tgcttccagc actccccctg ctaccgacct tgacttgtcc
    29641 gatattgtaa agcaatattc ccttatgccc ctggggtcta tatctcctat tttcaaaccc
    29701 ttctgtacat aagtgttatc tgcaataagc ccccttatta ctccgtctat attggatttt
    29761 accggctcat catctacaaa tgaaaccaca tctccctgct tcacaatatc tcctatctca
    29821 cgggtattcc ttgttatacc cgttaccggt gcacgaagca gccgttcttg tgaataaccc
    29881 attatatatc ccggaatccc cgtatcggtg caagctgttc ccttgtatat gacctttccc
    29941 aaatcatgcc ctctattagt ctcaaccacg gcatcaacgt ccgcccccgc ctcgaatccg
    30001 ggaccgacgc caattgtgac aggagccgac cctctatatg ttcccgtgtt tctttttgcc
    30061 aaaacagcat caacaatcac caatggtttt atctgttcaa gtatcttcat ttcactgtcg
    30121 cacagtaccg gtatatggct attattccat atttcatata tgtcttcgat acattccgct
    30181 tttaccgcac ggacaccttc cacttccgct tcacctttca gaacggcatt tgcaaaggaa
    30241 gaaaaacgcc ttacgaccgt tggtttctca atttcggtga gaagcagcct gaagccgcat
    30301 ctatgaagcc tgtgccctat tccggttgca atgtcccccg caccttttac gataataata
    30361 tcatccattt tcgccctcca gctttgttat tgcttcatat tcttcccagg tatcaatatc
    30421 caggcccggt ttcgcatcag caaaattgac agtaatgatt ccctctttca tttcttcaag
    30481 caatacgcgc cctcccgaat ctcccgagag gctcataagc ttatttctca gctttgaagc
    30541 aaatatcaca gggtttcctt ttattccatt atacagggga actacggcat tgcaatgccc
    30601 cggtaaaaat tttccaatca gtttattgat aatactttca tcgagaaagg gctgatcccc
    30661 cgcaataaac ataaaacctt cggcagaccg gcaggaattc ttaaccccca aaccgacaga
    30721 atggctttgc cccgtttcag gagcattatt ctccacaatt tttgcccggt atttcttccc
    30781 tatatcggcc acagaatcgc tgccgcacac taatataatt tctccaagct ccgattttga
    30841 ggccgctgct ataaccctct caattacggg aattccccca accggaagca gcagcttgtc
    30901 cgtacccatt ctcctggata atcccgatgc aagtataatg ccgctgatca tattttcaca
    30961 tccccaattt tgtaactact atcccgtcag gctcataccc catatcagaa agcctttgaa
    31021 taattttgcc ggctgcagac cattcctttt ccccgtctgc cttatttaac accacatatc
    31081 gttcagcccg ggaaggaacg gctttgaaca aaccctcttc atgggcgatt aatctgccga
    31141 taatatctgc atcgataata tccccctcac ggcagcccgt tatattgcag aagatttccg
    31201 tcctgtggac attttccggg catacctttt ttccgacaca atcaaggcca accagcccaa
    31261 gcactctgga agcaagggag ggtatgacag gctcatgctc cgcaggcgcc ttcagaggcc
    31321 tccctcttga accgtctcct tctacgatta tataatcaaa tatgtctttt aaaaaaaggg
    31381 tatccagaaa ttccggatca acccccagca gcttgccttc tctagaaagt gccctgccgt
    31441 aaacagttat accgcttctt atgctcttaa acaaatccgg cagcaaaaga tccgcgatta
    31501 ctgtttgaac attttgattc tctaaaggat aataaatagc tgtggtagtt gtgacaagaa
    31561 ccttttttcc cgtacccgaa agttctttgg ccagcctgaa cattgcggag gttttacccc
    31621 ctgctcccac caggcaaatc atctctttct tccatatgct gatatttact tgactatata
    31681 agctttccat atcttacatt ccaaatgtac agcaagggta tctgcagcgt ctttcaccct
    31741 aatgcttctc ttggaacctc tcctcctccg ttactatata gtcatcaata tttctatata
    31801 tatgggaaaa aatccttttt atagcgcagg aaaagtattg tatttttata aatgaattgt
    31861 atcaaagacg aataaatatc ccccggcatg gccgggggtt tattgttata cacaaaaata
    31921 caccttctgc attaatcctg acagaaggta tacataatgc aaatatttac cttagtacgt
    31981 tcttttgttc ggtgcctggc acataagatt taaaactgtc ccctgaaatc agctcagcaa
    32041 actcagctgt tttctgccgg actagcatgc tctgcagatt gtcctgtgtc cgtccgagtt
    32101 tttttgcact ctcccgctgc tggtgatata tatattccgt aagctcagct tttaattctt
    32161 catctgccgt tcgggaataa acctttaatg cgggatcaat ccccgcagga cccgactgat
    32221 acaatatttg tacatcaaag ctttttagtg ttcccgcaag gtaacggtca gcattatagg
    32281 aggcgacaaa accgtccgga ttaagcaggc aaagggcgca cataatcaaa gtacctgtaa
    32341 aagctgaaag ccgcacaatt gagaactgcc ttttctgcag tacaataacg ccgacaaaaa
    32401 caacggcaag gaataccatg aataaacagg gcagcaggcg gcgtatggaa agcccgtacg
    32461 ctcctatata aagcgccatc ttgctgaatg ctgcggcaat taacagcaaa gtcagcagag
    32521 aaagcaggca gttaagtgtt tttaatatat agttcttttg aggtttttta caaaataggt
    32581 ttgccaatgt cagcaatgtc aggttaatcg cagcaatttg gcacagctca aaaaatccgc
    32641 tgcgggcgta ttcggagtat atctgccacc cctccggcct ctgtcctgca aaggctgaga
    32701 aaaagtaagg aagctggctg ccgataaata ccatatacaa gcagcatatt aaaccgagca
    32761 cggtgtaaac agtagcggct gcaagtaccc tcatggattg aaccccatat tggatacctt
    32821 ctcttttaaa cgtattgcag cccctgttat gggcacagcc tgctacaagc ccaaataaat
    32881 aagctgctac aggtatcgaa aggataatgt tgaatattgt ttctgaaaac tgattctgca
    32941 tccatcggaa atattcaata agcccatttg caattctgga gaaccctccg ctgtccgctc
    33001 tcaaaagcag cggcaaaacc atgcccgcaa tcaataacgt cagcatcaat cccagtgcga
    33061 tggataaaaa ctgcttacca aagcttctct taatatgtat tgatgaataa aggcttttgt
    33121 attgacatcc gaagttttta aacggaataa caaacatggc attaatcccg tccagtatga
    33181 gccaattgct ggtattggca agaatcaatc gtcctgaggc actgattaca tagtaaacgg
    33241 ccccacagaa caggaacagg ctgcgccacg gctccaagcc attattagaa tataatgaat
    33301 agcttattcc tactaataca accacagcca gccaaaagaa acctgaacgg gtaatggtta
    33361 ctcctttttt tataaaatac attgttacgg caatgtaata tccaaatgta aacactgcaa
    33421 caccccatcc ctgccaggaa aacattaccc atcttacaaa gaaaaacccc agcacaaatg
    33481 caagcaccgc aaaaattgta tccctgctgt cagctatgaa aggttctttg gccccatatg
    33541 ccgccggtat ccctcctccg gctgcctgca gcacttcgtc ggcagccagt tgtccgttat
    33601 tacttaaaat attccctttc atatataatc tccttattct tagtcttact ccctgaattc
    33661 cagaatatca cccggctggc attccagagc tttgcagatt gcctccagtg ttgagaaacg
    33721 tattgctttg gctttac
//
LOCUS       DAOOAB010000027        33050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1729_length_33050_cov_15.588544, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000027 DAOOAB010000000
VERSION     DAOOAB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 33050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33050
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_33050_cov_15.588544"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PKU88_07300"
     CDS             complement(<1..1208)
                     /locus_tag="PKU88_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HPX69118.1"
                     /translation="MRLELGNIFIKDVQFGDATKVEKGTLYVNKAELLEAVSGDPRLT
                     KIEIDIAKPGEETRIMPVKDVIEPRVKVEGPGGIFPGMVSKVDVVGSGKTNVLKGAAV
                     VTAGKIVGFQEGIIDMSGPGAEYTPFSKTQNIVIVCTPVEKLQQHEHEQAVRMVGYKA
                     AAYIGEAGRKVVPDEIKVYETPTLMEGMKQYPNLPRVAYVYMLQTQGLLHDTYVYGVD
                     AKKIVPTFIYPTETMDGAIVSGNCVSACDKNPTYNHLNNPIIEDLYERHGKDFNFVGV
                     IITNENVTLADKERSSNWTAKLAEYLNLDAVIISEEGFGNPDTDLMMNCKKIAAKGIK
                     TVLVTDEYAGRDGASQSLADADKAADAVVTAGNANEIISLPPMKKVIGYLEPADTIAG
                     GFAGSLKADG"
     gene            complement(1268..1588)
                     /locus_tag="PKU88_07305"
     CDS             complement(1268..1588)
                     /locus_tag="PKU88_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPX69119.1"
                     /translation="MIELDKDNFDSEVLQATGPVLVDFWGEKCEPCKALMPEVHALAE
                     KYGDKMKMCKLNTTQNRRLAIGQKVLGLPTFIVYKNGEKIKEISGAENCTPEAVENLI
                     KENL"
     gene            complement(1602..2534)
                     /gene="trxB"
                     /locus_tag="PKU88_07310"
     CDS             complement(1602..2534)
                     /gene="trxB"
                     /locus_tag="PKU88_07310"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPX69120.1"
                     /translation="MGKVYDVVIIGGGPAGLSAGLYASRARMSAMIVEKAKWGGQAGT
                     TEELENYPGSIEMPTGPKLMERMKNQAEAFGTELVKDEVLSIDTSDKIKKIKLGSGNE
                     ISARTIIIATGAQPMMLGIPGEAELRGKGVSYCATCDADFFAELEVVVVGGGDAAVEE
                     AIYLTKFAEKVTIVHRRDQFRAAKSIAEKAMKNDKIAIMWDTVPEEIYGDGIVEGIKL
                     RNVKTNEVTDFRTDGVFMFVGTKPISDFAKGVVEMDQKGYIMADEEMRTSVEGIFAAG
                     DVRVKSLRQVVTAAADGAIAAVNAEKYIEKNFEN"
     gene            complement(2549..2914)
                     /locus_tag="PKU88_07315"
     CDS             complement(2549..2914)
                     /locus_tag="PKU88_07315"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HPX69121.1"
                     /translation="MLTNNSLVRDEYGKDLDIIFIDGEYIDVLKSARDMVHKGAKLIT
                     HPLMGSVKPNETPFRSIIVAQGDSLDIGSLTVMESSIASAEKFQRDVKVPLWNDRVLN
                     DFRFVDLKLFESAYDSITK"
     regulatory      complement(3102..3200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3305..4408)
                     /locus_tag="PKU88_07320"
     CDS             complement(3305..4408)
                     /locus_tag="PKU88_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPX69122.1"
                     /translation="MKSKVYFAGVHSNAGQESLLVKLRKLFDRAGFGEMISQNDIVAV
                     KIHFGEKGNTAYIRPTYARQIVDKIKEKGGKPFLTDSNTLYNGSRANGVDHYETAIYN
                     GFAYAVVNAPIIIADGILSKNSVDVEINKKHFDRVKISKEIASASKMIVMSHYKGHEN
                     AGFGGAIKNLAMGCAPAAGKQQQHSVVRPKVSRRCVGCENCLKYCPAGAITKEGGHAL
                     IGETCIGCGECISICPIRAINPQWDIEIPVFIERMTEYAYGAVKNKNGNAGFINVLMN
                     ITPLCDCYGFSGAYIVPDIGILASKDPVAIDAASYDLVNEQTGNINSSLKCNHGKGED
                     KFRGLYGNIDGTRQLSYGEEIGLGSREYELIKI"
     gene            complement(4420..5259)
                     /locus_tag="PKU88_07325"
     CDS             complement(4420..5259)
                     /locus_tag="PKU88_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPX69123.1"
                     /translation="MGIKIITDSICDVPKDYADRYGIRVMPLRVNFGNTSYIDGIDLT
                     LEEFLKKLEGAEVLPTTSQVTPGDFLAAYREETALGNKVISIHASSQLSGTHDSAVIA
                     KEQMPGSDIYVIDTMGISLGAGLLAIKAARLAEEGFEAEEVVKQIEASKQKMKYMLIL
                     DTLKYLQKGGRLSLPASVIGSILSIKPILTVVNGKLELLEKSRGMKKAIFSVMNRIKE
                     NGWSLNGKTVGINHITNLESRDILEEQLKKEFNIKEFIRGEAGTVIATHGGPGAVAVH
                     FEI"
     gene            complement(5283..5747)
                     /gene="trmL"
                     /locus_tag="PKU88_07330"
     CDS             complement(5283..5747)
                     /gene="trmL"
                     /locus_tag="PKU88_07330"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HPX69124.1"
                     /translation="MSLNIVLVEPEIPQNTGNIARTCAATGSRLHLVKPLGFSVDDKH
                     LKRAGLDYWGLVNIYYYDSFAELKSRYSDGLFFYSTTKAKKKYTEVIYKENCFIVFGK
                     ETAGLPKLLLEENKEFCIRVPMINNARSLNLANSVAIVAYEALRQIGFPGMH"
     gene            complement(5769..6236)
                     /locus_tag="PKU88_07335"
     CDS             complement(5769..6236)
                     /locus_tag="PKU88_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPX69125.1"
                     /translation="MNVEYINPFVNVSVNLMKMICNETATRGKIYIKDSTFSSDNVVI
                     IIGVAGEIKGQVFFSMNNTTACNIASTMMFGMEVKELDDMAKSAIAELGNMIMGNVST
                     EFFNNGTKIDITPPTILVGKEIAFSTKGLKTICIPLVLEKLGRIEIDVALTEY"
     gene            complement(6368..8071)
                     /locus_tag="PKU88_07340"
     CDS             complement(6368..8071)
                     /locus_tag="PKU88_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPX69126.1"
                     /translation="MNRSFKRISWLLTVVMILAMLLPMGGVWADESGAAAAGSVNLTI
                     VHTNDTHSRVLSSSSEIGFAKIASKVKEIRSSNENVLVLDAGDTFHGQTISSLVEGES
                     VVEVMNIIKYDAMVPGNHDFNYGQERLLELSQMTNFPIVAANITKADGSAFLSPYIIK
                     EFKGVKVGIFGLATPETTYKTNPNNVKGLTFEDPTAAAERMVQELEGKADIIIALAHL
                     GLDESSEYTSKLVAEKVDGIDLIIDGHSHTVLTEGLKVGDTLIVQTGEYDKNFGIVEL
                     ACENGSVTAAKASLITIEAAEQLPEDAEVLQAIEGIKAEQDVITSVVIGRTEVELDGV
                     REHVRAGETNLGNLITEAILKATGADAVITNGGGIRASIDTGDITKGEVITVLPFGNY
                     VVVKKIKGSDIIAALEHGVSQYPAPNGGFPHVAGIRFAFDPAAAAGSRITKAEIGGKA
                     LDPNAEYLLATNDFMAVGGDNYTMFAPGPVITELPGLDEIVMEYIANYGTADIKVDGR
                     ILAEEKKAQAAAEYEVRLGDVLWKIAEKFGLTYEKLAEYNKLKDANRIYPGQKLLIPE
                     F"
     gene            complement(8166..8999)
                     /locus_tag="PKU88_07345"
     CDS             complement(8166..8999)
                     /locus_tag="PKU88_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HPX69127.1"
                     /translation="MPFNPKRVLFEQDALKYPLGRELFDYFKGRDVQTGMLKSHNRVT
                     DIPGKTPAQAYSEGKNTLVVGVRRTLNFESCKPSAHYQLPLATGCMGMCMYCYLNTQL
                     GKKPYMRVYVNVEGILEAVHNGHTTVRFSLNAANVIKKYEHRTPEAEERVSAARKIAG
                     HDYPLGFIIAPVLLYEGWKKDYGELLNMLKDKLDTYINRSICFEIISHRFTSRAKKNI
                     SEIFPGNDLPMNEEERRFKYGQFGYGKYVYNREQLSDMESFFRERIAEIFPNSRIDYI
                     I"
     gene            complement(9076..9897)
                     /locus_tag="PKU88_07350"
     CDS             complement(9076..9897)
                     /locus_tag="PKU88_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPX69128.1"
                     /translation="MKKLFLFIAAVLAMGYMSFISMSIPAVLEKKSIEYGNDGSAANT
                     MPEIRVMTYNIHRGINKENKLDLNGISEAIRDSGAEIIALQEVERFSVRTGFRDQIKY
                     IADKLSMKYAYGKSINILNGQYGNAILSRFPIEEYEVCKLPSDGERRTILRAVLNVYG
                     NRIHFYSTHLGLDQEERDIQAEEIVRLAEEDRNSIVSGDFNSKADKLGMLTERYMDCA
                     SYENDDIRVTFEGEGLSERIDYIFVSRNFRVKSYEVLKSDASDHYPVVCTLDFID"
     gene            complement(9991..10824)
                     /locus_tag="PKU88_07355"
     CDS             complement(9991..10824)
                     /locus_tag="PKU88_07355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPX69129.1"
                     /translation="MMNRDSLKYKMLVTDMDYTLLNKEKRVSDRNREALRRAMEKGVH
                     MVVATGRIYTSARIYAKLLGLDTPIIASNGALIKDASKTIFRDILSQDTVREMLRLCH
                     KYGIYCHFFTENTIYSEKLINVSLRYTEWNKYMGEEDQVKIRIVDDGEEIIEAAKSEV
                     LKAVVFDDDDEKIQKLRDGIMETGIVSVSQSMKHNLEVMNKGVTKGNAVRILAQLYGI
                     NREEVIAIGDNENDISMIEYAGLGIAMGNAEECLKRAADHVTGDYQEDGVAEAIEKFI
                     L"
     gene            complement(10851..11456)
                     /locus_tag="PKU88_07360"
     CDS             complement(10851..11456)
                     /locus_tag="PKU88_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPX69130.1"
                     /translation="MGSVRVMPDSTCDLSPELIGKHGISVVPLYVIFNGDSFRDGIDI
                     KTDELYKMTERLNTLTVRNARITAQEFPEGRIEVIDSRNLSAGIGLLVMKAVDFAGQG
                     LGISEIEEGKMILEQKIRGKKEKALKSMPENTLTDKSIIDSRSVFVTHSMADEEAEYL
                     KGVLEQKLEGPEIIITNAGCVISSHCGRGTAGILYMESSEA"
     gene            complement(11626..11712)
                     /locus_tag="PKU88_07365"
     tRNA            complement(11626..11712)
                     /locus_tag="PKU88_07365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11676..11678),aa:Leu,seq:cag)
     gene            complement(11729..13993)
                     /locus_tag="PKU88_07370"
     CDS             complement(11729..13993)
                     /locus_tag="PKU88_07370"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HPX69131.1"
                     /translation="MNNRNIKEILFYFSLIFMMVFSFNLALKDFVGIDIHLIGVFLIS
                     LSASASASMLLLFPAAVSAGLIPAAGFLLFYCYKEPASVRLYLKEAQEFTTWLLGYLA
                     GYNYFEKAYSLLFIIVITLLAALITSTAVYKGKGVLPLILAGTAVMAYFWFVYVESAR
                     LYLALFLFASIMLYSYRTFKKRLREWIAAGCIIDYKAGRNWMICSGVVIAAALLFSAA
                     LPLKIGPVRWQWLNDKAVSLFPFITEWRNDVLESYSFSSAGYSDNKLGGEIRWDDSVV
                     MLVRTRGEEALYLRGTVMDRYYENSWNKSGKRYKEYKPGHLMPIPYGKDIKVYTRDLE
                     ISHRKLLTSTIFAPYSLYRIQHGGKRTYMDEDSEAYASKIAATDTSYRVTSIIPYIDV
                     AKLRLSGAETLEEDDLRRYTALPEDIPERVRSLSHRITNAYSSNYDKAKAIEKYLRQN
                     YMYSLKPPQLPSNKEFTDYFLFEGKEGYCTYFATSMAVLLRASGIPCRYVEGFIASYE
                     GSEEREVRGTDAHAWVEVFFDGYGWVTFEPTPQYPVVEILEPGSVKDEVFEGAANDTV
                     TVENKRAGMPGYFGQPEGAERVGNGTISGDIKKSYNFAKIILLIALSALTARVIFLYV
                     KQAITEIGLGRSSGKRFAADYIEDINRYLRRAGFEMKGSETLREFMNRVGLSFRENPG
                     HISKIITILEKTRYGGYEPCANERSILKVFRKEVKAFAIKKAGILRYFISMYVLGDRK
                     NAVQKFHVEFKKIT"
     gene            complement(13986..15095)
                     /locus_tag="PKU88_07375"
     CDS             complement(13986..15095)
                     /locus_tag="PKU88_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPX69132.1"
                     /translation="MYGIFLTALFLAIISYASGRYIYNNLVNLTWEISSNAVRGDRVS
                     LKTDWYNAGIIPIPYLKVIASLPKRLAGEEQKQRVYSIMPEMITTMSRDFECRHKGVY
                     KIGIIETVFEDTLGIFKWKKVFYDDRYLVVYPKVHLLKHLDIPMRQQYGTSAVKHAAY
                     EDCTGTKDIRKYIIGDSFKKIHWKVTAHRGDFYVRNVEINASADLIVFLDLYDYGFDE
                     ETAYDIEEKGAECAASIIRYALANSMSVNFTAKGEELVSLSAKRADRFNLFLDAVSKF
                     SSKGDIPIGDLVRKEARKLDWEATAVVITPCIEKNASAFLSLKASGTELVIIYLCGDG
                     ANGGKDAEILKSSGLNVFAVGLEDDINQVLGGHYE"
     gene            complement(15166..16116)
                     /locus_tag="PKU88_07380"
     CDS             complement(15166..16116)
                     /locus_tag="PKU88_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPX69133.1"
                     /translation="MIDKDLFIKKIEESVSKVIVGKKDVLEKLLVVLFAGGHVLIEDA
                     PGVGKTTMVHALARSTGCSFKRIQFTPDLLPSDITGVTVYNIKQGEFEFKHGPITSNI
                     VLADEINRGSPKTQSSLLEAMQERQITVDGNTWKLPEPFMVLATQNPIEYEGTFPLPE
                     AQIDRFAIRISMGYPTYNEEKSILNKCSSDKAPENIEPVISAEEVLEIRRLVEKVHAD
                     DSVQDYIVKLVRGTREHKDVYLGCSPRGTLALFSNCRALAYIRGREYVLPDDVKELVF
                     CTLAHRIILKSEAAIQGKDQYSVLAEILKSIKVPVANING"
     gene            complement(16399..17085)
                     /locus_tag="PKU88_07385"
     CDS             complement(16399..17085)
                     /locus_tag="PKU88_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HPX69134.1"
                     /translation="MNDSLYSKKAICPICGKQFSSMKAKVNSCKVKKKDEDFCIHYED
                     LNPMYYEVFVCPSCAYAAPENSFKELTEKEVKLLKEVFSGREVSRSFCGQRSLDDATA
                     AYKLAIHTAELRKASASVLAGLCLRLAWLYRFKGDKQEQLFLEYSLRNYLEAYDKEPL
                     PIGNLNEISLMYLLGELSRRLGKLDDAITWFEKAATNPEKKENPMIEKLAREQWALTR
                     EQHKESEKSE"
     gene            complement(17139..18524)
                     /locus_tag="PKU88_07390"
     CDS             complement(17139..18524)
                     /locus_tag="PKU88_07390"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPX69135.1"
                     /translation="MSRSMKIGIGVIIVLLIAAAALSAAMVSGDKIHSNISISGIDVS
                     GFVPSQAESMLKKKMEPIIDNLSIVLKFKGKEWQLKYRDIDFKYEYEDAVTKAYDIGH
                     KGSYFKRIKDVAATQLNGEDIKLNYSYDSQYLKNRLEDISKELEQEARDASIVLKEQG
                     FVITDEVEGRKLDVNKSCDIIKALLDKASEGEAELAVDVTEPEIKRSDLENIKDNLGE
                     YSTKFNAADVDRTHNIKIAASSASHILVKPGEVFSLNKSMGPRLAKFGYKEADVIINN
                     ELVPGIGGGVCQVSSTLYNAALLSNLKIVERKSHSLPSSYIALGRDATLSEDYIDLKF
                     ENNTKYPIYIYGEVKGSWVKFSIYGYDEYPERTVKIKTETIKVTPPQISIIEDPALPE
                     GTEVEEKKAYTGYVVKSYRVVMENGKEVSTEELPLSVYRVVNGVKRVGTMKVEPQPAE
                     NEVLPEVPGDI"
     gene            complement(18628..20616)
                     /locus_tag="PKU88_07395"
     CDS             complement(18628..20616)
                     /locus_tag="PKU88_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HPX69136.1"
                     /translation="MTEQDLLFSLDIGTRTVVGIVGFHDGQKYIIKDFEVEEHKERVM
                     YDGQVHDIELVAKAVEKVKERLEEKTGLKLKRVSVAAAGRTLKTCKGFAELDADPSVG
                     VDRELISNLEIEAIQKAQKSMEEQTEEGEQSFFCVGYAVVRYFLNGGIIGNLEGHKAK
                     RIGVEVLATFLPRLVVESLYAVVARAGLEVSSITLEPIAAMNVSISSSLRLLNLALVD
                     IGAGTSDIALTKDGTVFAFAMASIAGDEITEKIAQVLLLDFDEAEKAKISLNNEIIKY
                     TDIMGLIHEKNSSEVLNEIDESIRILAEEISNKILEFNGKAPSAVFLVGGGSQIPRLT
                     GYIAEYLRIPSERVGVRSTDIIRNVEIESKGLSGPEFITPIGIAVTSYFNRQKDFLHV
                     TVNGVNVKMLNTKALTIADSLIMMNYNPRNLIGRRGNPLTFKVNGREVIVRGEPGVPA
                     VIYLNNNIAGLDRPITNGDEIIVKPAVEGKPAEANVKDYLGDYIEKTVYLNENPLNLR
                     PMITINGLECNLDAPIKNSDEVNIENIDTVHGLLKSLNLNYNNIEVEINGKKAEPGDV
                     INDGDHISYKVLERTETESFRKPDKKTVCVNVTINGKPEVLVTNKTQCLFVDVFNNIE
                     IDFDSIKGVSAMLLNGNKASFTDVINDGDVIELHWKEK"
     gene            20798..21706
                     /locus_tag="PKU88_07400"
     CDS             20798..21706
                     /locus_tag="PKU88_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX69137.1"
                     /translation="MYDILKNCRICPRKCGSDRYEGKGLCGAGPEVLVAKACLHQWEE
                     PCISGGRGSGTVFFSGCNMKCVFCQNHDISQASSGALTGKKISTERLAEIMLELQEQG
                     AHNINLVSPTPYALHIIEAVRKAKEKGLHVPIIYNTNGYELEETIGILKGTVDVYLPD
                     IKYYDDLYSVKYSNTGSYFEYASKAVTAMHNQAGYPEFDKDGMIKKGLIIRHLVLPGL
                     GPDSKRIFQWVRENIGKYAYMSIMCQYTPMHKASQYNDINRRISLSEYDEVLGYFFEA
                     GLENGFMQGKESAHSIYTPEFDLSEI"
     gene            complement(22130..23044)
                     /locus_tag="PKU88_07405"
     CDS             complement(22130..23044)
                     /locus_tag="PKU88_07405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX69138.1"
                     /translation="MSAISAENMKSFSDTVSDLYTLFFRSYPFMKPFLMNIFLFSSGN
                     FGIIIKEEGREAGRFSVYHSAEGVIVKEGIKEGINVRITLNRKHYEDMMQNRKKYMEK
                     PYKLLKFFPAFLRSVAIDDRSLAENVLAGSRVTLRPGLDSDIYYLLKWYNDIELNKLA
                     GWSNSKATPDKLRYNISRSFGYDPMNLMIDNEEGTPIGTIQLYDFNEQDKSCKLGIRI
                     GDRDYWSKGYGEDAVRTILKYAFFNIDIFRVTLKVYEYNERAVKCYLKCGFRCEGRTR
                     QSACIDGKFYDEIIMGVLKSDFIRLSDK"
     gene            complement(23099..25471)
                     /locus_tag="PKU88_07410"
     CDS             complement(23099..25471)
                     /locus_tag="PKU88_07410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPX69139.1"
                     /translation="MTMGNEIRISVRDLVEFVYRSGDLDSRFAGMGKAIEGTRLHQKI
                     QGLRKDEAAGSGEIYLKEISVSMRICVRGITFIISGRIDGLLQGSEGITLEEIKTTSS
                     PLDIIGEDTYPVHWAQVMCYAYMYASEQALKTVNVRLTYYNADSGEQKAFIKRADIEE
                     LEKHFTEAVEKYYDWAYTACEWIALRNSTIRDMGFPYDKYRKNQRKLAVAVYKTITAS
                     KRIFIQAPTGTGKTISALFPAVKALGEGNTDKIFYLTAKTITRQAALDAISKMKCKEL
                     RIKAVVLTAKDKICFKEESDCNPEYCEYAKGYYDKVNAVLKEVFEKEDIITRELVEEY
                     AGRHMVCPFELSLDLSYWTDCIICDYNYVFDPRVSLRRFFTETESNYVLLIDEAHNLV
                     DRAREMFSAELYKKPFLEAKRLMKVKAPLISKAANGINSALLKIRKKSGSKNCCMAKE
                     ELKELCQALRAFISHSEEWLGSNHGEQGYREVLELYFEAAAFLRIYEIFDERYTAYVE
                     LPGSDVKLKLFCLDPSKLLGEITGRMKASIFYSATLTPLYYFKDILGGSEEDYNIRLD
                     SPFEESNLSLTVAGNVSTRYTDREGSLSEIADYIKTVVSARKGNYLIFFPSYKYMNEV
                     FGLFTERYPEIGTILQQPAMQEEEKERFLQCFCRDNEKELVGFAVMGGIFSEGIDLEG
                     ERLIGAVIVGVGLPQVCFERDIIMVFFKKKNNMGFEYAYMYPGMNKVMQAAGRVIRSE
                     SDRGVVLLIDERFCSSRYTGIFPREWLHNNNVESHEELRRILEEFWSRTE"
     gene            complement(25492..27204)
                     /locus_tag="PKU88_07415"
     CDS             complement(25492..27204)
                     /locus_tag="PKU88_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPX69140.1"
                     /translation="MRKYFLIIFIALIVAAITGFFVFPQLWTTVQYTPDKVHLVVEDG
                     IAGDEDSPILENGQLLFSIDTIKKFIDPNAHWDEKANKAVFTTKNKVLIMKTGKLTAM
                     VNARPVDINIPVRLVGNKPYIPIQMLDTMFGIEVQWIEENNVAVLDYAKNTVQIAVVQ
                     KDNRIMRAKPALWSPAVRKNIDKGEVLRVFGGDEKWYKVRSKDGFIGYMQKKYVKTDL
                     IASETDMTESAGSTAWKPERGKINMTWEYVGNRNPDVSQLKNISGLDVISPTWFYIVD
                     KQGTVANKADIGYVDWAHKNGHKVWALVGNGSDPDITHDFLNNTDTREKIYQQLLIYA
                     KLYKLDGINIDFENIYMKDRDMLTQFMRELAPLLREQGLVVSIDVTFRSSSENWSMCY
                     DRKALSKVVDYVAVMAYDQHWAASPIAGSVAELGWVERGLERILEEVPGEKLLLGLPF
                     YTRVWKEETIDGKIKVSSTAVSMGKVEQILSEKKPEVIWDEESGQYYAEYKEGKATYK
                     IWIEDDKSINLKSSLIHKYDLAGAASWRKGFESEDIWAVLEDNLKVKQNYAQWANANS
                     TAAD"
     gene            complement(27485..28672)
                     /locus_tag="PKU88_07420"
     CDS             complement(27485..28672)
                     /locus_tag="PKU88_07420"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPX69141.1"
                     /translation="MGLHDLDFITQRINELKEQGVYRKLPVLESPSGPRCIVNGKNVI
                     NLSSNNYLGLANHPRLREAGKKAIDKWGMGAGAVRTIIGTMSIHEELEEKLAKFKHVE
                     AVLVFQSGFTANAGAIPGIVDKGDVIISDELNHASIIDGCRLSKADVVRYKHSDMEDL
                     ERVIKEVKDNYNIKLIITDGVFSMDGDIAKIPEIVELAEKYGCLTYVDDAHSSGVLGK
                     SGQGSAHHFGLSDRVDVQIGTLSKAIGVVGGYVAGRRNLIDWLNHRGRPFLFSTAAPP
                     AVAAACIEAINILSESTELTDRMWDNANHFKKKLNELGFNTGKSETPITPVITGDDRK
                     AVELSKRLFEEGVFAQAIVFPTVPKGGARVRTIVTAAHTKDDLDEAAAAFAKVGRELG
                     LIK"
     gene            complement(28709..29668)
                     /locus_tag="PKU88_07425"
     CDS             complement(28709..29668)
                     /locus_tag="PKU88_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPX69142.1"
                     /translation="MKKILITGALGQIGTELTEYLREQYGNDNVIASNRSKHGDDSFF
                     EAGPFELLNVLDAKGLAEVCRKYGINQIIHLAAILSANAEKNPQTAYDINMNGTYNVL
                     EVARELGIAVFTPSSIAAFGANTPADNTPQDTIQRPSTMYGVTKVAGELLCDYYFKKY
                     GVDTRGVRFPGIISYAALPGGGTTDYAVHIYYEALKNKEYASYIGAGTYMDMMYMPDA
                     LKAVHDLMEANPDKLLHRNAFNVTAMSFEPEMLAAEIRKHIPEFILTYDVDPVRQAIA
                     NSWPNSLDDSAARAEWGWNPEYDLASMTKDMLEKLAVKLNIKE"
     gene            complement(29953..30705)
                     /locus_tag="PKU88_07430"
     CDS             complement(29953..30705)
                     /locus_tag="PKU88_07430"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPX69143.1"
                     /translation="MKFARYESQGIVSYGLVEDSTVYRIEGDIFGEYKVTEERYMLSD
                     VKLLAPCIPSKIVAVGVNYRDHASEMGHELPKDPVLFIKPSSSVIGPEGKIIRPGMSK
                     RVDYEAELALVIGKRTKNVEPSEAFQYVLGATCFNDVTARDLQPKDGQWTTAKSFDTF
                     APMGPFIVTGIDCNNTDIELVLNGETKQKSNTSYLINKTHELVSHVSKIMTLYPGDVI
                     ATGTPSGVGPMSAGDVVEVRLQGIGILRNYVE"
     gene            complement(30719..31504)
                     /locus_tag="PKU88_07435"
     CDS             complement(30719..31504)
                     /locus_tag="PKU88_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69144.1"
                     /translation="MYDKVKRETMVKTYFGCNIEDIASIVILTPVWSLESFISKADEV
                     SAEFAGWYKGVTLKFNSKSITVISSGIGAPRSGDCALALSYTDCDTVIFSGSAGAVSQ
                     CCSIGDMLIVSEAVIGEGYSRYHSGDIKRDRFGELVQGDKETAQKLLNEVLKYQSRYG
                     INCREGRVFSIDSLLGERKDTFDYMQEKGCDGVEMEVSAVFTACRCAGKKAAALIIIS
                     DLPLKYKNLFEGIMETDMKRYNEIEHDLPEILLEAAAGFRQIK"
     gene            complement(31528..32907)
                     /locus_tag="PKU88_07440"
     CDS             complement(31528..32907)
                     /locus_tag="PKU88_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69145.1"
                     /translation="MKKVFAVLAALIMVLSGCSGETPGNGTEPDDIDIKNERLVKEFT
                     GTVNIFHRSSDNLLLSAEEGELFKYYEIDIGTAEVRESKLTEMADRYFYYEPIGNKGF
                     IAVEDTDYKNTLKFIEKGGTEHVIAEDMGPADAINISISPNAGSVAYTSLLEGNDIYG
                     IYVYDTETFENKRLINIKSDGFIEGFHYLVNWSPDENNIIINDKYIYDAFSGSKKGEL
                     KSAYSQWSPTGSKIAFVLEDESGQWLNAEDYHMFQGKKVCVYDIFKGSYDEVFEINGD
                     ECIFGDITWGGKDRFLAFPGIKAGDTDIPDWYMKLNYSSVYIVDLEEGGSKRLETNVD
                     ASDGTMIELGNLKFSNQGNLLSFTVGNYEKSSLHIVNTATLQAESYDNAEYLHWIEGE
                     YYLISAKGDTMYFCMDNSIIGIDENLQEDVKYTSKSKLDDFYMSEDGRGILIFELQED
                     IHIARYVGE"
BASE COUNT         8489 a         8409 c         5672 g        10480 t
ORIGIN      
        1 cttccgtctg ccttaaggct gcctgcgaat ccgcctgcaa ttgtatctgc cggttcaaga
       61 taacctataa ccttcttcat tggcggcagt gaaataattt catttgcatt acctgccgta
      121 accactgcat ctgctgcttt gtctgcatct gcaagggatt gcgaagcacc gtccctgcct
      181 gcatattcgt ctgtaacaag aacagtcttg attccctttg cagctatctt cttgcagttc
      241 atcataaggt cggtatcagg atttccgaaa ccttcttctg atattatgac tgcatcaaga
      301 ttcaaatatt ctgcaagctt tgccgtccag ttggaggacc tttccttatc ggcaagagta
      361 acattttcat tagtaattat tactcctaca aaattgaaat ccttgccatg cctttcataa
      421 agatcctcaa taataggatt gttcaaatga ttgtatgtcg gattcttatc gcaggctgat
      481 acgcagtttc cgctgacaat tgctccatcc attgtttcag ttggatatat gaacgtagga
      541 acaatcttct ttgcatccac accgtataca taagtgtcat gaagcaatcc ctgtgtctga
      601 agcatgtata catatgccac tctcggaaga ttcggatact gtttcatacc ttccattaat
      661 gtcggagtct catatacctt tatttcatca ggtactactt ttctgcctgc ttctccgata
      721 taagcagctg ccttgtaacc caccattctc actgcctgct catgctcatg ctgctggagc
      781 ttttccaccg gtgtgcagac aataacaatg ttctgagtct ttgagaatgg tgtatactct
      841 gctccggggc cgctcatgtc tatgatgcct tcctggaatc ccacaatctt tcctgcagta
      901 acaactgcag caccctttaa tacgtttgtt ttgccagagc cgaccacatc taccttgctg
      961 accataccgg ggaatattcc gccggggcct tctactttaa ctctgggctc aattacgtct
     1021 tttacaggca ttattcttgt ttcttcacct ggttttgcaa tgtcaatctc aatttttgtc
     1081 aatctggggt caccgctgac cgcctccaaa agctcagcct tgttgacata aagagttccc
     1141 ttttcaacct tggtagcatc accgaattgt acatccttaa tgaaaatatt acccaactct
     1201 aaacgcaaaa cgttcacctc cctaataatt tgagtaattc aataaataca gtttattata
     1261 gcttttctta aaggttttct tttataaggt tttcgacagc ttcaggggtg cagttttctg
     1321 ctccgctgat ttctttaatc ttctccccat ttttgtatac gatgaaagtt ggaagtccca
     1381 ataccttctg cccgattgcc agtcttctgt tctgagtagt attaagcttg cacattttca
     1441 ttttatcccc gtatttttca gccagagcgt gcacctccgg cattaatgct ttgcagggtt
     1501 cacatttttc tccccaaaaa tcaaccaata caggacctgt tgcttgaagc acttctgaat
     1561 caaaattatc cttgtctagc tctatcatat tttcacctcc tttaattttc aaaattcttt
     1621 tcaatatatt tttcggcgtt gaccgctgct attgcgccat ctgcagctgc cgtaacaacc
     1681 tgtctcagtg atttcaccct tacgtccccc gctgcgaata ttccctcaac tgatgttctc
     1741 atttcttcat ctgccattat ataccccttt tggtccattt ctacaacgcc cttggcaaaa
     1801 tcagatatcg gtttggtgcc tacgaacatg aatacaccgt cagtcctgaa atctgtaact
     1861 tcattagtct tcacattcct gagcttaata ccctctacta tcccgtcgcc gtaaatctct
     1921 tccggtactg tgtcccacat tatagcaatc ttgtcgtttt tcattgcctt ctctgctatg
     1981 gactttgcag ccctgaactg atctcttcta tgtactattg tgaccttttc cgcaaactta
     2041 gtaaggtata tggcttcctc aaccgccgca tctccgcctc cgacaaccac aacttccaat
     2101 tcggcgaaga aatcggcatc gcatgttgca cagtaggata caccttttcc ccgaagctcc
     2161 gcctctcccg ggatccccag catcatcggc tgagcgcctg ttgcaattat aattgttctt
     2221 gcgcttattt cattaccgct tcccagcttt attttcttta tcttatctga tgtatcaatg
     2281 cttaatactt cgtctttcac aagttcggtt ccgaaggctt ccgcctggtt tttcattctt
     2341 tccataagct tgggtcccgt cggcatttca attgaacccg gataattttc aagttcttcc
     2401 gtagtacctg cctgtccgcc ccatttagcc ttttcaacta tcattgcact cattctggct
     2461 ctcgatgcgt acaatcccgc tgaaaggcct gcaggtcctc cgcctattat aacaacatca
     2521 tataccttgc ccatttatac tcctcccttt actttgtaat actgtcataa gcactttcaa
     2581 aaagcttcaa gtccacaaat ctaaaatcat taagtactct gtcattccat aacggtactt
     2641 tgacatccct ttggaatttc tccgctgaag ctatactgct ctccatgact gtaagagacc
     2701 cgatgtccag agaatctcct tgtgcaacaa taatgcttct gaacggagtt tcattcggct
     2761 tgacgctccc catcaacgga tgagttatca gctttgctcc tttgtgcacc atatccctgg
     2821 ctgacttcag cacatcaata tattccccat caataaatat tatatccagg tctttgccat
     2881 actcatccct taccagactg ttgtttgtca gcacaataat atttctgtcc agccctatcc
     2941 ctccaactaa atattttggt tacggatatt tatccaatta aacaaaaaga agtagagact
     3001 atgcctctac ctctgtataa tacctgagat attctttgat agatgagcaa agacctcatt
     3061 tatcaagttg accctccggt gcctacgctt ttcagagttg cgtccggtaa tcggtccttt
     3121 tgcctgagag cttcatcgta aagcatcttt ttatgacttg ccccttcggc gccaaaactg
     3181 gtctctcccg aaaaccatca cccgcagtat ttattttttc tgcttttata taatatggaa
     3241 ataatctgta tggcttatat tcgtttttgt tatacattta gtatataaga attttattct
     3301 cttgtcaaat ttttatcagt tcatattccc tgctgcctaa tccgatttcc tctccatagg
     3361 ataactgtct tgttccgtca atatttccgt ataaacctct gaacttgtct tcccctttgc
     3421 catgattgca tttcaagcta ctgttgatgt ttcctgtctg ttcgttcaca agatcatagg
     3481 atgcggcatc tattgcaaca ggatccttgg aagcaagtat ccctatatca ggtactatgt
     3541 atgcaccgct gaagccgtaa caatcgcaca gcggagtaat attcatcagt acattaataa
     3601 atcctgcatt tccattttta ttttttactg ccccgtaagc atattctgtc attcgctcaa
     3661 tgaataccgg tatttcaata tcccattgag gatttatcgc cctgatggga catatggaaa
     3721 tgcattcgcc gcagccgatg caagtttccc cgatcagtgc atgccctcct tcttttgtaa
     3781 ttgcacctgc cggacaatac ttcaggcagt tttcgcatcc aacgcatctc cgtgatactt
     3841 tcggccttac cactgaatgc tgctgctgtt ttcccgctgc gggggcgcaa cccatggcca
     3901 ggttcttgat tgccccgccg aatccggcat tctcatggcc tttataatgt gacatcacta
     3961 tcatcttgga tgcactagca atttctttgg aaatctttac tctgtcaaaa tgctttttgt
     4021 ttatctcaac atcaacggaa tttttgctta gtattccgtc tgcaattatt atcggagcat
     4081 tgacaactgc ataagcaaag ccgttgtata ttgctgtctc atagtgatcc actccgtttg
     4141 cccttgaacc gttatacaac gtattggaat ccgtcaggaa gggttttccc cctttttctt
     4201 tgatcttatc aactatttgc cttgcataag taggcctgat atatgccgta tttcctttct
     4261 ctccaaaatg aatctttacc gctacgatat cgttctgtga aatcatttct ccgaatccgg
     4321 ctctgtcaaa gagcttccgc agcttgacaa gcaaactctc ctgccctgca ttggaatgta
     4381 ctcctgcaaa atatacttta gatttcatca tatccgctcc taaatttcaa agtgtaccgc
     4441 tacagcaccg ggacctccgt gtgtggctat cactgttccg gcctctcccc ttataaactc
     4501 tttaatatta aactcctttt taagctgttc ctccaaaata tccctgcttt caaggttcgt
     4561 aatatggttt attccgactg tcttgccgtt taaagaccag ccgttttctt ttatcctatt
     4621 catcacagaa aatattgcct ttttcattcc ccttgacttt tcaagcagct ccagtttccc
     4681 gtttactact gtaagaatcg gttttatgct tagtattgag cctatcaccg aagcaggaag
     4741 cgaaagcctt ccgccctttt gaagatactt taaggtatcc agtattaaca tgtatttcat
     4801 cttctgctta ctggcctcaa tttgttttac tacttcttcc gcttcaaagc cttcctcagc
     4861 cagcctggca gctttgatcg ccaacaaccc ggcgccaagg ctgattccca ttgtatctat
     4921 aacatatata tctgacccgg gcatttgctc ctttgctatt accgctgaat catgtgtacc
     4981 gctgagttgt gaagaggcat gaatggaaat aaccttattc ccgagggctg tttcctcccg
     5041 ataggccgcg agaaagtctc ccggcgttac ttgtgatgtt gtaggcagaa cctctgcccc
     5101 ttccagcttt tttaaaaact cttccaaggt caaatctata ccatcgatat atgaggtatt
     5161 gccaaaattc accctcaagg gcatcaccct tatgccgtat ctgtcggcat agtcctttgg
     5221 aacatcacat attgaatctg ttataatctt tatacccata aacattcctc catgcggaaa
     5281 atttaatgca tacccggaaa ccctatctgc cttaacgcct catatgcaac aattgctacc
     5341 gaatttgcca gattcagtga tcgggcatta tttatcatag gaactcttat gcagaattcc
     5401 ttattctctt caagaagaag cttcggcaaa ccagcagttt ccttgccgaa gactatgaag
     5461 cagttttcct tatatataac ttccgtatac tttttctttg cttttgtggt tgaataaaag
     5521 aacaatccgt cagaataccg gctcttaagc tctgcaaatg agtcataata ataaatattc
     5581 accaagcccc aatagtccaa ccctgctctt ttcagatgct tatcgtccac cgaaaaaccc
     5641 aggggcttga caagatgaag ccggcttcct gtagccgcac aggttcttgc aatatttccc
     5701 gtattttgag gaatctcagg ctctaccaat acaatattca aactcaagct aacaactcct
     5761 gtgaatgatt aatactccgt caatgcaaca tctatttcga ttctccccag tttttccaat
     5821 accaggggta tacatatggt tttcaatcct tttgtcgaaa aggctatttc ctttcccacc
     5881 aatatcgtcg gaggcgtaat gtcaatcttt gttccattgt taaaaaactc tgttgataca
     5941 ttgcccataa tcatattccc aagctcggca attgcactct tagccatatc atccaattcc
     6001 ttgacttcca ttccgaacat catggttgaa gcaatattgc acgccgtagt gttattcatg
     6061 ctaaaaaaaa cctgcccctt gatttctccc gccacaccta taattattac aacattatca
     6121 gaggaaaagg ttgagtcttt tatgtatatc tttccccgtg tagctgtttc attgcatatc
     6181 attttcatta ggttgactga tacattgaca aaaggattaa tatattctac attcaaatat
     6241 gcatcctcca aatatgttca atttgactat ataatatata ataccctatt attccttcat
     6301 tgccaataat taaaggtcaa aaaagaaaga aactgccgga aatgaaaccg gcagtttcgt
     6361 attatgatta gaactcagga atcaacagct tctggccggg ataaatcctg ttggcatctt
     6421 tcagcttgtt gtactcggcc agtttttcat atgtcaatcc gaatttttca gctatcttcc
     6481 aaagaacatc cccaagcctt acctcgtatt cagctgctgc ctgggctttc ttttcctctg
     6541 ccagtatcct tccgtcaact ttaatatccg ctgtaccgta attggctatg tattccatta
     6601 caatctcatc cagacccggc agttccgtta taacagggcc cggcgcaaac attgtataat
     6661 tatctcctcc gactgccata aagtcattgg ttgcaagcag gtattccgca tttggatcca
     6721 gagccttacc tcctatttcc gcctttgtta tcctgcttcc tgcagcagcc gccggatcaa
     6781 atgcaaatct tatccctgca acatgaggga atcctccgtt aggagcggga tactgcgata
     6841 cgccatgctc aagtgctgca atgatatcag atcctttaat tttcttaact accacatagt
     6901 tcccgaaggg cagtacagta attacttccc ccttggttat gtctcccgta tctattgaag
     6961 ctctgatacc gccgccattt gtgatgactg catccgcgcc tgttgctttc agtattgctt
     7021 cagttatgag gttgccaaga tttgtttctc ccgccctgac atgctctctt acgccatcaa
     7081 gttcaacttc tgtcctgcct attaccactg atgttatcac atcttgttcc gccttaattc
     7141 cttcaatagc ctgaagcact tcagcatctt caggcaattg ctccgctgcc tctattgtga
     7201 taagtgaagc cttcgcagcc gttacgctgc cgttttcaca agccaattca acaataccga
     7261 aattcttgtc atattcaccg gtctggacta taagcgtatc gccaaccttt aggccttccg
     7321 tcagaaccgt atggctgtgc ccgtcaataa tcaggtcaat gccgtcaacc ttttcagcta
     7381 caagcttgct tgtatattca gaggactcat ccaatccaag atgtgcaaga gcgataatta
     7441 tatcggcctt tccttccagt tcctgaacca ttctttctgc tgcagccgtc ggatcttcaa
     7501 aagtcaagcc ctttacatta ttggggtttg tcttgtatgt tgtttccgga gtagccaggc
     7561 cgaatattcc tacctttacc cctttgaact ccttaataat ataaggagac agaaaagcac
     7621 tgccgtccgc cttggtaata tttgcagcaa ctatagggaa gtttgtcatt tgactaagtt
     7681 caagaagcct ttcctgtccg tagttgaaat catgatttcc cgggaccatg gcatcgtact
     7741 ttattatatt catgacttcg acaacacttt caccctcgac aagtgaggaa atggtctgcc
     7801 catggaatgt atctcctgca tcaagcacca gaacattctc attggaactc cttatctcct
     7861 tgaccttgga tgcaatcttt gcaaaaccga tttcactgct gcttgaaagc actctggagt
     7921 gtgtgtcgtt agtatgaact atagttaagt tcacactgcc ggcagctgcc gcaccgctct
     7981 catccgccca tacgccgccc attggaagaa gcatagccag aatcatcaca acagtaagca
     8041 gccagcttat tcttttgaaa gacctgttca taatcatacc cccacaataa ttatttgcat
     8101 gtaccataat ttgtacatac atgaatacat ttcaattata tcataatttt gtaattttga
     8161 tacactcata tgatataatc aatcctgctg ttaggaaaaa tttctgcgat tctttccctg
     8221 aaaaagctct ccatatccga aagttgctct ctattatata catatttgcc atagccgaat
     8281 tggccgtatt tgaagcgcct ttcttcctca ttcatcggca ggtcattgcc gggaaatatt
     8341 tcgcttatat ttttctttgc tctggaagta aacctgtgtg aaattatttc gaagcatatg
     8401 ctcctgttta tgtacgtatc aagcttgtct ttcagcatat tgagcagctc tccgtaatcc
     8461 ttcttccatc cctcataaag caagacggga gctattataa accctaaggg atagtcatgc
     8521 ccggcaattt ttctcgccgc tgacaccctt tcctccgctt ccggcgttct gtgctcgtat
     8581 ttcttaatta catttgcagc gtttagactg aaccttacag tggtatgccc gttatgcaca
     8641 gcctcaagta ttccttccac attcacatat acccgcatat aaggcttctt acccaactgt
     8701 gtgttcagat agcaatacat gcacataccc atacaacctg ttgcgagagg aagctggtaa
     8761 tgggcagaag gcttgcagct ttcaaaattc aaggtccttc tgacaccgac tacaagtgtg
     8821 ttcttgcctt cggaatacgc ctgcgccggt gtcttgcccg gaatatccgt gactctgtta
     8881 tgagacttca acatacctgt ttgaacatcc ctgcctttaa agtaatcaaa tagttcccgc
     8941 cccaatgggt atttcaaagc atcctgttcg aataaaaccc gttttggatt aaagggcata
     9001 gcatcaaatt ctttcctcga aatatgcgca gcctttgccg catacttatt atctgcaaaa
     9061 aaaaaatgac ttgcgtcaat ctatgaaatc caatgtgcag accacaggat agtggtctga
     9121 tgcatccgat ttaagcactt catatgactt taccctgaaa tttcttgaaa cgaatatgta
     9181 atctatacgc tcactcagcc cttccccttc aaaggtcacc ctaatatcat cattttcata
     9241 gcttgcacag tccatgtatc tttcggtgag catcccaagc ttgtccgctt ttgaattaaa
     9301 atcccctgaa actatggagt tcctgtcttc ttcagccaac cttacaatct cttcagcctg
     9361 aatatctctt tcttcctgat caagtccaag atgagtgctg taaaaatgta tcctgtttcc
     9421 gtatacattg agcaccgccc ttaaaattgt ccttctctcc ccatcggaag gcaatttgca
     9481 tacttcatac tcctcaatgg gaaatctact caatatcgca tttccgtatt ggccgttcaa
     9541 tatgttaatg ctttttccat aggcatattt cattgaaagc ttgtctgcaa tgtatttgat
     9601 ctgatcccgg aatccggtcc tcaccgagaa cctttccacc tcctgaaggg caattatctc
     9661 tgctcccgaa tcccttatgg cttctgatat tccgttcaag tccagcttat tctctttatt
     9721 tatccccctg tgaatattgt acgtcatcac tcttatttcc ggcatggtat tggcagcact
     9781 gccgtcattc ccatactcaa tgctcttttt ctccaaaaca gcaggaatgc tcatactgat
     9841 gaaactcatg tatcccattg ccaatactgc agcgataaaa agaaaaagct ttttcatgct
     9901 gtttcttccc tttcaaagta tttgcttacc ctcaacatta tgttaacata atattgagcc
     9961 ttcgtaaaac aaatattttg aaaatttgaa tcataatata aacttttcta tagcctccgc
    10021 cactccatcc tcctgataat cccctgtaac atgatcagca gccctcttca agcattcttc
    10081 cgcatttccc attgctattc caaggccggc atattcaatc atgctgatat cattttcatt
    10141 atcgccgatt gcaataacct cttcccggtt tattccgtac agctgtgcaa gtatcctgac
    10201 ggcatttcct ttggtaacgc ctttgttcat cacttccaga ttgtgtttca ttgattggct
    10261 tacagatact attccggttt ccataatacc atcccttagc ttttgtatct tctcatcatc
    10321 gtcatcaaac acaacagctt ttaatacttc ggatttagcc gcctcaataa tttcctctcc
    10381 gtcatcaaca attcttattt taacctggtc ttcttcgccc atgtacttat tccactctgt
    10441 ataacgcaat gatacattta taagcttttc cgagtaaatc gtgttttctg tgaaaaagtg
    10501 gcaatagatt ccgtacttgt ggcataatct cagcatctcc cttacagtat cctgactcaa
    10561 aatatccctg aaaatagtct tggaggcgtc ttttatcaac gcgccattac tggcaataat
    10621 cggtgtatcc agtcccaata attttgcata tattctggcg gaggtataga tccttcctgt
    10681 tgcaactacc atatgcacac ctttctccat tgcccttctc aacgcttccc tgtttctgtc
    10741 agatactctc ttttccttgt tgagaagcgt ataatccata tcggttacca gcattttata
    10801 tttcaaactg tctctgttca tcattttcct ccgcatttgc tcttaagtat ttatgcttct
    10861 gaactctcca tataaagtat acctgcggtt ccccggccgc agtggcttga aatcacacat
    10921 cccgcgttgg taattattat ttcgggccct tccagctttt gttcaagtac acctttaaga
    10981 tactcagcct cttcatccgc cattgaatgg gtgacaaaaa cactccggga atcgataata
    11041 ctcttatctg tcaaggtatt ctccggcatg ctttttaatg ccttttcttt tttacccctt
    11101 attttttgtt caagaatcat cttgccctct tcaatttcag atattccaag gccttgtccg
    11161 gcaaaatcaa ctgctttcat aaccagtaac cctatcccgg cggaaaggtt tcttgaatct
    11221 atgacttcta ttcttccctc aggaaattcc tgtgctgtaa tgcgggcatt ccggacagtt
    11281 agtgtattca gcctttctgt cattttatac agttcatctg tttttatgtc tattccatcc
    11341 ctaaatgaat ccccgttgaa aataacataa agcggcacaa ccgaaatgcc atgctttcca
    11401 ataagctccg gagacaaatc gcatgtacta tccggcatta ccctcacgga ccccatttaa
    11461 ctaacaacct cctgaaaaat aattatgtac atatttatta tacatctata aaattaaaac
    11521 ttcaaaatgt acaaacttcc caaacaattg gaaattagca ttatatctct ataaaataaa
    11581 acaaaaagag ctataacctt tgctatagct ctttaccatc atatttggtg cgcgaggcgg
    11641 gatttgaacc cgcacagcct tgcgaccgct agaacctgaa tctagtgcgt ctgccagttc
    11701 cgccactcac gctaacggaa ttattatatc atgtaatttt tttgaattca acatgaaatt
    11761 tttgtactgc attttttctg tctccaagga catacatgct tataaaatag cgtaaaatac
    11821 cggctttttt tattgcaaag gccttcactt cttttctgaa aaccttgaga atgcttctct
    11881 cattcgcaca aggctcataa ccgccgtatc tggttttttc caatatcgtt ataatctttg
    11941 aaatatggcc gggattttcc ctgaaactta atcccactct gttcataaat tccctgagcg
    12001 tttccgaccc tttcatctca aatccggcac gtctcaaata tctgttaata tcctctatat
    12061 aatctgcagc aaaccttttc ccgctgctgc gtccaaggcc aatttccgtt attgcctgct
    12121 ttacatacaa aaatattact cttgcggtta gagctgacaa agcgatcagt aatattattt
    12181 ttgcaaaatt gtatgatttt tttatatcac cgctaattgt accgtttccg accctttccg
    12241 caccctcagg ctgcccaaag tatccgggca taccggctct tttattttcc actgtaactg
    12301 tgtcattggc agctccctca aatacctcat cctttacact cccgggctca agaatttcga
    12361 ccaccggata ttgcggggta ggttcaaagg ttacccaccc gtatccgtca aagaaaacct
    12421 ccacccaggc gtgtgcatcg gtacccctta cttctctttc ttcagatcct tcatagcttg
    12481 ctatgaatcc ttccacatac ctgcagggta ttcccgaagc cctcagcagt accgccatgg
    12541 aggttgcaaa gtatgtgcag tagccttctt ttccttcaaa aagaaaatag tcagtgaatt
    12601 ctttatttga aggcagctgc ggcggtttaa gagaatacat ataattttgg cgcagatatt
    12661 tctctatggc tttagccttg tcataattgc tgctatatgc atttgttatt ctatgggata
    12721 atgaccttac cctctcggga atatcttccg gcaaggcagt atagcgcctt aaatcatctt
    12781 cctccagagt ctctgccccg gacaacctca gcttcgcaac atcaatgtaa gggattatgc
    12841 ttgtcaccct gtatgacgta tctgttgcgg caattttgga agcatatgcc tccgaatctt
    12901 catccatata tgtcctcttc ccgccgtgct gtatccgata taatgaataa ggggcaaata
    12961 tggtagaagt cagaagcttc ctgtgtgata tctccagatc ccttgtataa acctttatat
    13021 cttttccgta tggaataggc attaaatggc ccggtttgta ctctttatac ctctttcccg
    13081 acttgttcca gctgttctca taatatctgt ccataacggt tcccctgaga taaagcgctt
    13141 cttccccccg tgtccttaca agcataacca ctgaatcatc ccagcgtatt tccccgccca
    13201 gcttattgtc cgaatatcct gcggagctga agctgtatga ctccagtacg tcgttcctcc
    13261 attcagtaat aaaggggaac aggctcacgg ctttatcatt aagccactgc caccttacag
    13321 ggccaatttt taagggcaga gctgcagaaa acaataaagc cgctgcaatt acaactccgg
    13381 aacaaatcat ccagttccgc cctgctttgt aatcgataat acagcctgcc gctatccatt
    13441 cccggagcct cttcttaaat gtcctatatg aataaagcat tatggatgca aacaggaata
    13501 atgcaagata caacctggcg gattcgacat atacaaacca aaagtaggcc attactgcag
    13561 tacccgcaag aataagcgga aggactcctt tccccttata tacggcagtt gaagtaatca
    13621 atgcggccag aagtgtaatg actatgataa acagcaagga atatgccttt tcaaagtaat
    13681 tgtaacccgc cagatacccc aggagccatg ttgtaaactc ctgtgcctct tttaaataaa
    13741 gcctcaccga tgcaggctcc ttatagcagt aaaaaagcag aaaccccgct gcgggaatca
    13801 aacctgccga aacagccgcc ggaaacaaaa ggagcatcga tgctgaagct gatgcagaca
    13861 gagaaatcaa aaacactcca atcaaatgaa tatctatccc gacaaaatct ttaagtgcca
    13921 ggttaaagct gaaaaccatc ataaatatca ggctgaaata gaacagtatt tcttttatat
    13981 tcctgttatt cataatgccc tcccagtacc tgatttatat catcctccaa accgaccgca
    14041 aacacgttaa ggccgctgct cttcagtatt tctgcgtcct ttccgccatt agcgccgtct
    14101 ccgcacaggt atattattac aagctctgtc cctgaagctt tcaaagagag aaatgcggaa
    14161 gcattctttt cgatgcaagg ggttataact actgcagtag cttcccagtc cagttttctt
    14221 gcttcttttc tgaccaggtc cccaatgggt atatcccctt ttgatgaaaa cttgcttacg
    14281 gcgtcaagga acaaattaaa cctgtcggct cttttcgccg aaaggcttac cagttcctct
    14341 ccctttgccg tgaaattcac ggacattgaa tttgccaaag catatcttat aattgatgca
    14401 gcacattccg ctcctttttc ttcaatgtca taagctgttt cctcatcaaa tccataatcg
    14461 tacaaatcaa gaaatacgat taaatccgcg gaagcattga tttcaacatt tcttacataa
    14521 aaatctcctc tgtgtgcggt taccttccag tgtatttttt tgaagctgtc cccgattatg
    14581 tatttcctta tatccttggt gccggtgcag tcctcgtatg cagcatgctt gacagctgaa
    14641 gtaccgtact gctgtctcat agggatgtcc agatgcttca ataaatgcac cttgggatat
    14701 acaacaagat atctgtcgtc gtaaaaaacc tttttccact tgaatatgcc caatgtatcc
    14761 tcgaatacag tttcaattat tccaatttta taaacgccct tatgcctgca ttcaaaatct
    14821 ctggacatag tggttatcat ttcgggcatt atggaataca ccctctgttt ttgctcttca
    14881 ccggcaagtc ttttgggaag gctggctata accttcaaat aaggaattgg tataatgcct
    14941 gcattatacc aatcggtctt aaggcttacc ctgtcccccc ttactgcatt actgcttatt
    15001 tcccatgtga gattaacaag gttattatat atgtacctgc cggaggcata ggatattatt
    15061 gccagaaata aagctgtcag aaagattcca tacataaata tgcctcctgc aaaaagtgct
    15121 gcagcaaaaa tgagcagaat caatgcaagg gcttttttat tcatctcaac cattaatgtt
    15181 cgccaccggt actttaatac tttttaagat ttccgcaagt acggagtatt gatccttacc
    15241 ctgtattgca gcttcggatt tcaatatgat cctatgggca agagtgcaga atacaagctc
    15301 ctttacatca tcaggcagca cgtattccct tccccggata taggcgagtg cacggcaatt
    15361 gctgaaaagt gccagtgttc cccttggact gcagccaagg tacacatcct tgtgttccct
    15421 tgtaccccgg accagcttta caatataatc ctgtacggaa tcatccgcat gcactttttc
    15481 aaccagtctt ctaatttcca gtacttcctc cgcgctgata acaggttcta tattctccgg
    15541 cgctttatcc gaactgcatt tgttcaaaat gcttttttct tcgttataag tgggataacc
    15601 catggatatt ctgattgcaa acctgtctat ctgtgcttca ggcagcggaa atgtgccttc
    15661 atactctatg ggattctggg ttgcaagcac catgaagggc tccggcagct tccatgtatt
    15721 tccgtcaact gttatctgcc gctcctgcat agcctcaaga aggcttgact gtgttttagg
    15781 agaacctctg tttatttcat ccgccaaaac aatatttgat gttatcggtc catgcttgaa
    15841 ctcaaattcg ccttgcttta tgttgtagac cgtaacccct gttatatctg acggaagcag
    15901 gtcgggagtg aattgtatcc tcttgaagct gcagccggtt gatcttgcaa gcgcatgaac
    15961 catagtagtc ttgcctactc cgggagcatc ctcaataagc acatggcctc ccgcaaacaa
    16021 aactaccaat agtttttcca acacatcctt cttgccgacg ataacctttg atacactttc
    16081 ctcaatcttt tttatgaaca agtccttgtc aatcataata cattcctcac tgatttacga
    16141 taaaattgat gcataaacaa ttcctgaaac tgccatgata cattgaaatg cctataataa
    16201 tattctattt aatatattca gacaattcaa tcaactaaaa gtgaaatttt ttttaaaatt
    16261 atttcacaaa ttatgaataa tgcgacgcga gggcattggg aggacacggg gacgtttctc
    16321 ctgtcccgct ttcagcggga caggagaaac gtccccgtgt cctccccgtg tcctcttgga
    16381 cagtttttag ctttatttct attcggattt ttcactttcc ttgtgctgct cccgagtcaa
    16441 tgcccactgt tctcttgcca gcttctcaat catggggttt tctttctttt cgggatttgt
    16501 tgctgctttt tcgaaccagg tgatagcgtc atcaagcttc ccaagccttc ttgacagctc
    16561 acccaaaaga tacatcaagg atatctcatt cagattcccg attggaagcg gttctttatc
    16621 ataagcttcc aaatagttcc ggagcgaata ttccaggaac agctgctcct gcttgtcccc
    16681 cttaaatctg taaagccatg caagcctaag gcacaatccc gcaagcacac tggcactcgc
    16741 ttttctcagc tcggcagtat gaatggccag cttgtacgca gctgtcgcat catccaggct
    16801 tctttggcca cagaagcttc tgctcacttc tcttcccgaa aagacctcct taagaagctt
    16861 gacttctttt tccgtcagtt ctttgaaaga attctccggg gctgcgtatg cacaggaagg
    16921 acatacaaaa acttcataat acatggggtt caagtcctcg taatggatgc agaaatcctc
    16981 atccttcttc ttaactttac aggaattgac cttggctttc attgaagaaa actgcttgcc
    17041 gcatatgggg caaatagctt tcttggaata cagcgaatcg ttcataaaac attctccaat
    17101 ccaagtacta ttgtcatgac agctttaatg cactttagtt atatatcacc gggtacttcc
    17161 ggcaaaactt cattttcagc cggctgaggc tcaaccttca tggttcccac tctttttacc
    17221 ccgtttacaa ccctgtaaac acttaatgga agctcctcgg ttgaaacctc ttttccgttt
    17281 tccataacca ccctgtagct tttgacaaca tacccggtat atgccttctt ttcttccacc
    17341 tctgttccct ctggaagagc aggatcttct attatgctta tctgaggggg agtcacctta
    17401 attgtctctg tctttatctt gacagttctt tccgggtatt cgtcatagcc gtatatgctg
    17461 aatttaaccc agcttccctt cacttctcca taaatgtaaa tgggatattt ggtgttattc
    17521 tcaaatttta aatctatata atcctctgat agtgttgcgt ctcttcccaa agctatatag
    17581 ctggatggca gggaatggct cttgcgctct actattttaa gatttgaaag cagtgcggca
    17641 ttatataagg tggaagaaac ctggcatact cctcctccga tgccgggaac cagctcatta
    17701 tttattatga catcggcttc cttgtatccg aactttgcaa ggcgtgggcc catgctctta
    17761 ttcagggaaa agacctcacc gggcttaacc aatatgtgtg atgcactgct ggcggcgatt
    17821 tttatattat gagtcctgtc aacatcagct gcattgaact ttgttgaata ctctccaaga
    17881 ttgtccttta tattttcaag gtcggatctt ttaatttccg gttctgtaac atcaacagcc
    17941 aattccgcct ctccttccga ggctttatcc agcagagctt taataatatc gcaggattta
    18001 ttgacatcaa gcttcctgcc ctctacttca tcagtaatta caaagccttg ctccttcaat
    18061 actattgatg catccctggc ttcctgttcc aattcttttg aaatgtcctc cagtctgttt
    18121 ttcaagtatt gtgaatcata ggaataatta agcttgatat cctctccatt cagctgagtt
    18181 gcagccacat ccttaattct cttgaaataa ctgcccttat gccctatatc ataagctttt
    18241 gtcactgcat cttcatactc gtatttaaaa tcaatatccc tatatttcaa ctgccattct
    18301 tttcctttaa atttcaagac aatacttaga ttgtcaataa taggctccat ttttttcttc
    18361 agcatgctct ccgcctgtga aggaacaaaa ccgctcacat caattccgct aattgaaata
    18421 ttggaatgga ttttatctcc gcttaccatt gccgcactca gcgctgcagc cgcaatcaat
    18481 aaaacaataa ttacaccgat acctattttc atgcttctgc tcatatgcat cccccactct
    18541 tctgcgttta caactgcatc tctacttgta ctgatatatt atatacgttt ccattgccca
    18601 ttaatgatat atcaactaaa attcctgcta tttttccttc cagtgaagct caattacgtc
    18661 cccgtcattt atcacgtcgg tgaaactagc tttattcccg tttaaaagca tagcacttac
    18721 tccctttatg ctgtcgaaat ctatttcaat attgttaaat acatccacga acaagcattg
    18781 ggttttgttt gtcactaaaa cttccggttt tccgtttata gtcacgttca cgcagactgt
    18841 ttttttatca ggcttccgaa agctctccgt ttctgttctt tcaagcactt tatagctgat
    18901 atggtctccg tcatttatga catcgccggg ctctgccttt ttgccgttga tttcaacttc
    18961 tatattatta taattcaaat tcagagattt caaaagccca tgtactgtat caatattttc
    19021 tatattcact tcatcactat ttttaatagg agcatccaaa ttacattcaa gaccgtttat
    19081 agtaatcata ggcctaagat taaggggatt ttcatttaaa taaactgttt tttcaatata
    19141 atcgcctaaa taatccttta cattagcctc tgccggttta ccttctacgg caggcttcac
    19201 aattatttca tctccgtttg taatgggcct gtcaagtcct gcaatattat tattaagata
    19261 tatgactgca ggtactcccg gttctcccct gacaattacc tctcttccat tgaccttgaa
    19321 ggtcagcgga tttcccctcc tgcctataag gttgcgggga ttgtaattca tcattatgag
    19381 actgtctgct atggtaagag cttttgtatt aagcatcttt acgtttacac catttacggt
    19441 tacatgaaga aaatcctttt gcctgttaaa ataagaagta acagcgattc ctataggtgt
    19501 tatgaattcc ggtcccgaaa gccctttgct ctctatctcc acattcctta tgatatcggt
    19561 gcttcttacc cctactcttt ccgaaggtat ccggaggtat tcggcaatat atccggtaag
    19621 ccttggtatc tggcttcctc cccctacaag gaataccgca cttggcgctt tcccgttgaa
    19681 ttcaagtatt ttattactta tctcttcagc aagtatcctg attgattcgt ctatttcatt
    19741 caaaacctcc gatgagttct tttcatgaat cagtcccatt atatcggtgt atttgataat
    19801 ttcattattc aggctgattt tggctttttc cgcctcgtca aaatcaagca gcaagacttg
    19861 ggctatcttt tcggttatct catctcccgc aatagaggcc attgcaaaag caaatacagt
    19921 tccgtccttt gtcagtgcaa tatctgatgt tcccgctcca atgtccacaa gagcaaggtt
    19981 tagcagcctt aagctgctgg aaatcgacac attcatcgcc gcaataggtt ccaaagtaat
    20041 gcttgaaacc tccaggcccg cccttgcgac tactgcatac aggctttcca ccaccagcct
    20101 gggaagaaaa gtagccagaa cttccactcc tatgcgtttt gctttatgtc cttcaagatt
    20161 gccgattata cccccgttaa gaaaatagcg caccacggca taccctacgc agaaaaatga
    20221 ctgttccccc tcttctgtct gctcctccat gcttttctga gctttttgga ttgcttcaat
    20281 ttcaagattg cttattaatt ccctgtcaac tcccacagac ggatccgcat ccagctccgc
    20341 aaaacctttg caagtcttca gggtccttcc tgcagcagca acggatactc ttttaagctt
    20401 aaggcctgtc ttttcttcaa gcctttcctt aactttttcc actgcttttg caacaagctc
    20461 aatatcatga acttgtccgt catacataac cctttccttg tgctcctcca cttcaaagtc
    20521 cttgattatg tatttctgac cgtcatggaa tcccactatg ccgacaacgg ttctggtacc
    20581 tatatccaaa gagaatagca aatcctgttc agtcataaaa ctcacccttg caccgttaat
    20641 tttgcttaat tttacatttt tcctgcaatt tttcagcata gtagtattct atacgtgcat
    20701 aaataattcc ttcaaatttg cttctccata tgatttaata tttctatcgg aaggtttgaa
    20761 accttctgat aaactgtgga gcggaaaggg aatcaccatg tatgatatat tgaaaaattg
    20821 cagaatctgc cccagaaaat gcggttccga cagatatgaa ggcaaaggct tatgcggtgc
    20881 cgggccagaa gtgctggttg caaaggcctg ccttcatcaa tgggaggaac cctgcatcag
    20941 cggcggcagg ggatccggca cagtgttttt cagcggctgc aacatgaaat gcgtgttctg
    21001 ccagaatcat gacatcagtc aagcatcctc cggcgcatta accggcaaaa aaatctcaac
    21061 tgaaagattg gcggagataa tgctggagct tcaagagcag ggtgctcata atatcaacct
    21121 tgtatctccc actccatatg cactgcatat tatcgaggct gtaagaaaag caaaagagaa
    21181 aggcctgcat gtgccgatca tatacaacac caacggttat gaacttgaag aaaccatcgg
    21241 aattcttaag ggaactgttg atgtctacct tcccgacatc aagtattatg atgacttata
    21301 ctcggtaaaa tattcaaata ccggcagtta ttttgaatat gcctcaaagg ctgtaacagc
    21361 aatgcataat caggcagggt accctgagtt tgacaaagac ggaatgatta aaaagggact
    21421 gattatcagg cacctggttc tccccggttt aggaccggat tcaaaaagaa ttttccaatg
    21481 ggtacgtgaa aatataggaa aatacgcata catgagcatc atgtgccaat atactccaat
    21541 gcacaaagcc tctcaatata atgatatcaa caggagaata agcttgtccg aatatgatga
    21601 agtgcttggc tatttctttg aagctggcct ggaaaacggg tttatgcagg gcaaggaatc
    21661 tgcacacagc atctatactc ccgaattcga tttatccgaa atttaaaaat actattattt
    21721 tcatggttta ataaatacaa aaaaaagcaa cctattaata caatacattc aaaggcttac
    21781 ataaataccg aaaggaagtg ctatcgttat gcgaagagcc tccagaagca tgatgacttg
    21841 gggagcatta ggtgcaatgg caggattggt tttaatccct gcattaagtt caaaaactcg
    21901 caaaaaggtt tcacgttcag cgagaaatgc ttatttcaga gtatcagata tgatgcagga
    21961 tatgaaagac atgggaaaca agtgataaga cacggggggc atggggacgt ttcgggacac
    22021 ggggacgttt gggacacggg gacgtttctc ctgtcccgaa aagcgggaca ggagaaacgt
    22081 ccccgtgtcc caaacgtccc cgtgtcccgt ccccatgtcc ccccgtgtct tatttatccg
    22141 acaaccggat aaaatcgctt ttcagtaccc ccattataat ttcatcatag aacttcccgt
    22201 ctatgcatgc cgactgcctt gttctgccct cgcagcggaa gccgcatttg agatagcatt
    22261 tgactgccct ctcattgtac tcatatacct tcaatgtaac tctgaatata tcaatattga
    22321 agaatgcata cttaagaatt gtcctgactg catcctcccc atatcctttg ctccaataat
    22381 ccctatcccc gattcttatg ccaagcttgc agcttttatc ctgttcattg aaatcataga
    22441 gctgtattgt ccctataggc gttccctctt cattatcaat cataaggttc ataggatcat
    22501 acccaaagct tcttgagata ttgtacctca acttgtccgg agttgccttg gaattgctcc
    22561 agcctgcaag cttgttcaac tcaatgtcat tataccactt taacaaataa tatatgtcgc
    22621 tgtctaagcc gggtctcaag gttactctgc ttcctgcaag tacattctcc gccagggatc
    22681 tgtcatcaat tgctacagat cttaaaaagg ccgggaaaaa cttaagcagc ttatagggct
    22741 tttccatata ctttttcctg ttctgcatca tatcctcata gtgctttctg ttcaatgtga
    22801 ttcttacatt gatcccctct ttgattcctt ctttgacaat aacgccttcg gcactgtggt
    22861 aaactgaaaa cctgcctgcc tcgcggcctt cttccttgat gattattcca aagttgccgg
    22921 agctgaacag gaatatattc atgagaaaag gcttcataaa gggatagctc ctgaagaaca
    22981 gagtatatag atctgataca gtgtctgaga agcttttcat attttcagct gaaatcgcac
    23041 tcatttttaa ttactcctaa aactgtcagc aataataata taattattat atcatatatt
    23101 actccgtccg actccaaaac tcctccaata ttcgtcttaa ttcctcatgg ctttctacat
    23161 tattattatg cagccattcc cggggaaaga taccggtata ccggctgctg caaaaccttt
    23221 catctataag cagcacaacg ccccggtcag attcagacct gatgaccctc ccggctgcct
    23281 gcatcacttt attcatcccc ggatacatat atgcatattc aaaacccata ttgttcttct
    23341 ttttaaagaa aaccatgata atatctcttt cgaagcatac ctgcggaagc cctacaccca
    23401 caataacagc cccaataagc ctttccccct caagatctat tccttccgaa aaaattcctc
    23461 ccatcactgc aaatccaacc agttcctttt cattatcccg gcagaagcat tgaagaaagc
    23521 gctccttttc ttcctcctgc atggcaggct gctgcaaaat tgttccgatt tccggatatc
    23581 tctcggtaaa taggccaaac acctcattca tatacttgta tgaagggaaa aatatgagat
    23641 aattgccttt tcttgcactt actacagtct tgatgtaatc ggctatttcc gaaaggctgc
    23701 cctctctgtc cgtatatctg gtggacacat tgccggcaac agtcaggcta aggttggatt
    23761 cctcgaaggg agaatccagc cttatgttat aatcctcttc gctgcctccg agaatatcct
    23821 taaaataata caagggcgtc aaagtagccg agtagaagat ggaagccttc atgcgtccgg
    23881 ttatttcccc aagaagcttg gaaggatcca ggcaaaaaag ctttagcttt acatcacttc
    23941 ccggcagctc aacatatgca gtatatcgtt catcaaaaat ctcgtaaatc cttaagaatg
    24001 ccgctgcctc aaaataaagc tccagtacct cacggtatcc ttgttcccca tgatttgaac
    24061 caagccattc ctcactatgg ctgataaaag ccctaagggc ctggcacagt tctttcagct
    24121 cctcttttgc catgcagcag ttcttgcttc cgcttttctt ccttatttta agcaaagctg
    24181 agttgatacc attagctgcc tttgatatca aaggtgcttt taccttcatc aagcgctttg
    24241 cttcaaggaa aggcttctta taaagctccg cagagaacat ttctcttgca cggtccacaa
    24301 gattgtgcgc ttcgtctatc agcagcacgt aattgctttc tgtttccgtg aaaaaccttc
    24361 tgaggcttac cctggggtca aaaacataat tgtagtcgca tataatgcag tccgtccaat
    24421 aggataagtc caacgaaagc tcaaaggggc acaccatatg ccttccggca tactcctcca
    24481 ccagttccct agtaattatg tcctcctttt caaaaacctc cttcaatact gcattcacct
    24541 tatcgtaata gccctttgca tattcgcagt actcgggatt gcagtcggat tcctccttga
    24601 agcatatctt atcctttgcc gtcagcacaa cggctttgat tctgagttcc ttgcacttca
    24661 tcttgctgat tgcatccaag gctgcctgcc tggttatggt ttttgcagtc agatagaata
    24721 tcttatcggt attgccttct cccaaagcct taactgcagg aaaaagcgcc gagattgtct
    24781 ttcccgttcc tgtaggggcc tgaatgaaaa tcctcttcga agcagttatt gtcttatata
    24841 ctgcaactgc aagctttctc tgattcttcc ggtatttgtc atatgggaat cccatatccc
    24901 ttatggtgct gtttctcaat gcaatccatt cgcaggctgt ataagcccaa tcataatact
    24961 tctccaccgc ttcggtaaaa tgtttttcca gttcctcgat atcggccctc ttgataaaag
    25021 ccttttgctc gccgctgtcg gcattataat aggtcaggcg gacattgacc gtttttaggg
    25081 cttgctccga tgcatacatg taagcatagc acattacctg tgcccaatgc accgggtaag
    25141 tgtcttcgcc tataatatcc aacgggctgg aagttgtctt tatttcctcc agggtaatgc
    25201 cttcactgcc ttgcagcaat ccgtcaattc ttccggaaat aataaaggta atgcccctga
    25261 cacatatcct catggaaaca cttatttcct tgaggtagat ttctccgctg cctgccgcct
    25321 catcttttct caaaccttgt attttctggt gcagtctggt tccttcaatg gcttttccca
    25381 ttcctgcaaa ccgcgaatcc agatcccctg agcggtaaac aaactccacc aaatccctta
    25441 ccgatattct tatttcattt cccatagtca tgcaacatca gcctttgcaa attaatcagc
    25501 agctgtacta ttggcattag cccattgggc ataattctgc ttcaccttca ggttgtcctc
    25561 aagcacagcc cagatatcct ccgattcaaa gccctttctc catgacgccg cccccgccag
    25621 gtcatacttg tggattagag atgacttgag atttattgac ttgtcatcct ctatccaaat
    25681 tttatatgtg gccttgcctt ccttatactc ggcatagtac tggccgcttt cttcatccca
    25741 tattacttcc ggcttctttt cagacagtat ttgttccacc ttccccatgg atactgctgt
    25801 tgacgatacc ttaatcttac catctatagt ttcttccttc catacccttg tatagaaggg
    25861 aagtcccaaa agcagctttt cccccggaac ctcttcaaga attctttcaa ggcccctttc
    25921 aacccatcca agctctgcaa cagagcctgc tatcggactt gcagcccaat gctgatcata
    25981 tgccattact gcgacataat caaccacctt ggacagggct tttctgtcat agcacatgga
    26041 ccaattctca ctggaagacc tgaaggtgac atcaatcgac actaccagcc cctgttccct
    26101 taacaaaggt gccagctctc tcatgaattg agtaagcata tccctgtctt tcatatatat
    26161 gttttcaaaa tcaatgttaa tcccgtcaag cttataaagt ttggcatata tcagcagctg
    26221 ctgatatatt ttttcccttg tgtcggtatt gttcagaaaa tcgtgagtaa tatccggatc
    26281 ggatccgttt cctacaaggg cccacacttt atggccgttt ttgtgggccc agtccacata
    26341 gccgatatca gccttatttg ctaccgttcc ctgcttgtcc acaatataga accaggtggg
    26401 ggatatcaca tcaaggccgc tgatattttt cagctgtgat acatccggat tcctgttccc
    26461 tacatattcc cacgtcatgt ttatctttcc ccgctccggt ttccaggcag tgctgccggc
    26521 agattcagtc atatccgttt ctgaggcaat gaggtcagtt ttgacatatt tcttctgcat
    26581 ataccctata aacccgtcct tgcttcttac cttgtaccac ttttcatccc cgccgaatac
    26641 tcttagcact tcacccttgt caatattttt tctgactgcc ggggaccaca aggcaggctt
    26701 tgccctcatg attctgttgt ccttctgaac cactgcaatc tgaacggtat tttttgcata
    26761 atcaagcacc gcaacattat tttcctcaat ccattgaact tctattccaa acattgtgtc
    26821 cagcatctgt atgggtatat acggcttgtt gcccacaagt cttacgggta tattaatatc
    26881 cacaggacgg gcatttacca ttgcagtgag cttcccggtc ttcattatca gcactttatt
    26941 ctttgtagta aataccgctt tattggcttt ttcatcccaa tgggcatttg gatcgatgaa
    27001 tttttttata gtatcaatgg agaacagcag ttggccgttt tccaaaatag gtgaatcctc
    27061 atcccctgca atgccgtctt cgacaacaag atgcactttg tcaggagtgt attgaacggt
    27121 tgtccaaagc tgcgggaaaa caaaaaaacc tgtaattgca gcaactatta aagctatgaa
    27181 tattattaaa aagtattttc tcatttttac acccccggat aaaaatagat actattatta
    27241 tatcattagt aatgacgtaa tgcctctggc aaatatttca gccatcagtt tgcagtcata
    27301 ttcccagagc aaaagccttg gggacatggg gacgtttctc ctgtcccgct gaaggcggga
    27361 caggagaaac gtccccatgt ccccaaactt atagctgtgt cgcatcattc ttcagataag
    27421 aaaaaggggc gccgctgact gcttaccgtg tagttttgcg tcgccccctt gatattaccc
    27481 tgtattattt tatcagtccc aactctcttc caacctttgc aaaggctgcc gccgcctcat
    27541 caagatcatc ctttgtatgt gctgctgtca ctattgtcct tacccttgcc ccacccttcg
    27601 gaactgtcgg gaatactatt gcctgtgcaa atactccttc ttcaaaaagc cttttgctca
    27661 gttccacggc ttttctgtcg tcccctgtta ttaccggtgt aatcggggtt tcactctttc
    27721 ctgtgttgaa gcccagctca ttgagctttt tcttgaaatg atttgcgtta tcccacattc
    27781 tatcagtaag ctctgtactt tctgaaagta tgtttattgc ttcaatacaa gctgccgcta
    27841 ctgcaggtgg tgccgcagtg ctgaagagga aaggccttcc tctgtgattc agccagtcta
    27901 taagattcct tcttcctgcc acatatcctc ctacaactcc tatagccttc gaaagagtcc
    27961 ctatctgtac atctaccctg tcactcagcc cgaagtgatg cgcagagcct tgaccgcttt
    28021 tcccaagcac tccgctggaa tgcgcatcgt caacataagt gaggcatcca tatttttctg
    28081 caagttcaac gatttcggga atctttgcta tatcaccgtc catgctgaaa acaccgtcag
    28141 tgataataag ctttatgttg taattatctt taacttcttt tattactctt tccaaatctt
    28201 ccatgtcgct gtgcttatat cttactacgt cagctttgct cagcctgcag ccgtctataa
    28261 tggaagcatg attcagctcg tcgctgataa ttacatcccc tttatcgact atcccgggaa
    28321 ttgctccggc atttgccgta aagcctgact ggaataccaa aacagcttcc acatgcttga
    28381 acttggccag tttctcttcc agttcctcat gtatggacat tgtacctatg attgttctta
    28441 ccgcaccggc tcccatgccc catttatcaa tagccttttt ccccgcttcc ctgagtctcg
    28501 gatggttggc caaacccaga taattattgg aggaaaggtt gattacattc tttccgttaa
    28561 ctatgcatct cgggcctgag ggactctcca ataccggaag cttcctgtaa acaccctgct
    28621 ctttaagttc gttaatcctt tgagttataa aatccagatc atgcaaaccc attctgttta
    28681 cctccccttt ccttaatacc ttatatactt attctttaat attcagcttt actgccagct
    28741 tttccagcat atcctttgtc atcgacgcca aatcatattc aggattccat ccccattccg
    28801 ctcttgcagc actgtcatcc agggaattag gccagctgtt tgctattgcc tgtctgacag
    28861 gatcaacatc ataggtcaat ataaattcag gtatatgctt cctgatttct gcggcaagca
    28921 tttcaggctc aaagctcatt gcagtaacat taaacgcatt cctgtgcaaa agcttgtcag
    28981 gattcgcctc catgaggtca tgtactgcct ttagtgcatc aggcatatac atcatatcca
    29041 tatatgtacc ggctcctata taggaagcat attccttatt cttcaatgct tcgtaataaa
    29101 tatgcactgc gtagtctgta gtgcctcccc ccggcaaagc tgcataggag attatgcccg
    29161 ggaatctcac ccctcttgta tccacgccat atttcttgaa gtaatagtca cagagaagtt
    29221 ctcctgcaac ctttgttacc ccgtacattg tggaaggcct ttgtatagta tcctggggtg
    29281 tattgtcagc cggggtgtta gccccaaaag ctgctatcga gctgggggta aatactgcta
    29341 tacccagttc ccttgccact tccagcacat tatatgttcc attcatattg atatcatatg
    29401 ctgtttgagg attcttctca gcatttgccg aaagaattgc tgccaggtgt attatctggt
    29461 ttatgccata tttcctgcac acttcagcca atccctttgc atccagaacg ttaagcagtt
    29521 caaaagggcc tgcttcaaag aaactgtcat caccatgctt gcttctgttg ctggcaataa
    29581 cattatcgtt cccatactgc tctctgagat attcggtaag ttccgtgcca atctgtccca
    29641 aagctccggt aataagaatc tttttcatac aaatcctccc atctttatct gataactcta
    29701 ttttgacaaa atcagacgca tcaactaatt ttatactttc attatatcac ctataattca
    29761 aaaaaaaagt gtttttgtgc aaattttgca tacactattt atttaatgta tctatttcgt
    29821 gtacaattgt tctcaatttt cagacatttg acagcttaac atgcacttga gttaatacgg
    29881 aacagcagca ggaaagcagg agaggtacaa aaaataaagt gcagatttct gcactttatt
    29941 tttatggatt attcattcta cataatttct aagtatgcct ataccctgca gcctgacctc
    30001 aaccacatca cctgccgaca tgggccctac tccggaaggt gttccggtag caataacatc
    30061 accgggatac aaagtcatta ttttagatac atggcttact aattcatggg ttttgtttat
    30121 taaatatgat gtatttgact tttgctttgt ttcgccgtta agcacgagtt ctatgtcagt
    30181 attgttgcag tcaatccctg ttactataaa aggccccata ggagcaaagg tatcaaagga
    30241 ttttgcagtg gtccactgtc cgtcttttgg ctgcagatcc cttgcggtta catcattgaa
    30301 gcaggttgca ccaagcacgt attggaaagc ttccgacggt tcaacgtttt tcgtccgttt
    30361 tccgattacc aatgccagct ctgcttcata atcaaccctt ttggacatac cgggccggat
    30421 tatttttccc tccgggccga tgactgatga tgaaggcttt ataaaaagca ccggatcctt
    30481 tggcagttca tgccccatct ctgatgcatg atccctgtaa ttgactccta ctgccactat
    30541 ctttgaaggt atacatggag caagaagctt gacatccgaa agcatatacc gctcttccgt
    30601 aaccttgtac tctccaaaaa tatccccttc aatcctgtag accgtactgt cttccaccaa
    30661 accgtaactg acaattcctt gactttcata tctggcaaat ttcatctgcc gcacctcctt
    30721 attttatttg ccggaaacct gcagcagcct ccagcagtat ttccggtaaa tcatgctcaa
    30781 tctcattata ccgtttcata tcagtctcca taataccctc aaaaagattt ttgtatttaa
    30841 gaggcagatc actgataatt atcagtgcag ctgccttttt acctgcgcat ctgcatgcag
    30901 tgaatactgc cgatacctcc atttcaaccc catcgcagcc cttctcctgc atataatcaa
    30961 aggtatcctt cctctctccc agtaatgagt ctatcgagaa tactctgcct tcgcggcaat
    31021 taatcccata cctgctttga tacttcaata cctcattcag aagcttttgc gccgtttctt
    31081 tgtctccctg caccagttca ccaaaacggt ctctttttat gtctccgctg tgataacgtg
    31141 aataaccctc cccaatgaca gcttcgctta ctataagcat atcccctatg ctgcagcact
    31201 ggctgactgc tcctgcagaa cctgagaata tgacagtatc gcaatcggta taacttaagg
    31261 caagagcgca atcaccggat ctgggtgctc ctattccgct gcttatcact gttatgctct
    31321 tgctgttgaa ctttaaggtt acacccttat accagccggc aaactctgct gatacttcat
    31381 ctgccttgct gataaaactc tcaaggctcc ataccggtgt aagtataaca atgcttgcaa
    31441 tatcctcaat attgcagccg aaataagtct tcaccatagt ttctctcttg accttatcgt
    31501 acacatgaac accccgctct atacgtctta ttctccgaca taccttgcta tatgaatatc
    31561 ttcctgtaat tcaaaaataa gaattcctct tccatcctca gacatataaa aatcatccag
    31621 ctttgatttt gatgtgtatt tgacatcttc ctgaagattt tcatctattc ctataattga
    31681 gttatccatg caaaaataca tggtgtcccc ctttgcagaa atcaaatagt actccccttc
    31741 aatccaatgc agatattcag cattatcata tgattctgcc tgaagggttg cggtattcac
    31801 tatatgcagg ctgctttttt cataattccc aaccgtaaag ctaagcaggt ttccctgatt
    31861 ggaaaactta agattcccaa gctcaatcat cgttccgtcg ctggcatcca catttgtttc
    31921 cagccttttg ctgccgccct cttccaaatc cacaatatat accgagctgt aattaagctt
    31981 catataccaa tccggtatat cggtgtcccc tgcttttata cccgggaatg caagaaacct
    32041 gtctttccct ccccaggtta tatccccgaa aatgcattca tcgccgttaa tttcaaatac
    32101 ctcatcatag ctgcccttga aaatatcata gacacacacc tttttaccct gaaacatatg
    32161 gtagtcctca gcattaagcc actgtcccga ttcgtcttcc aatacaaaag caattttgga
    32221 acctgtaggt gaccactgcg aatatgcgga tttcaattct cccttttttg atccgctgaa
    32281 agcatcatat atgtatttgt cattaataat gatgttattc tcatccggag accaattgac
    32341 cagatagtga aaaccttcta tgaatccatc gctctttata ttaatgagtc ttttgttctc
    32401 aaaggtttct gtatcataca catatattcc gtatatatca ttgccctcca gaagagatgt
    32461 ataagcaaca ctgcctgcgt ttggagatat tgagatgttt atggcatcag ccggccccat
    32521 atcctctgca ataacatgct ccgtgccgcc cttttctatg aattttaatg tgttcttgta
    32581 gtctgtatcc tccactgcta tgaacccctt gtttccaatg ggttcataat aaaagtacct
    32641 gtccgccatc tcagtcaatt tactttccct gacttctgca gtacctatat ctatttcata
    32701 atacttaaaa agctcaccct cctctgcact gagaagcagg ttgtcagagg atctgtgaaa
    32761 tatatttact gtccccgtaa attctttcac aagcctttca ttctttatat cgatatcatc
    32821 cggttcggtt ccgtttcccg gagtctctcc gctgcagccg gaaagcacca tgataagtgc
    32881 agccaaaact gcaaacacct ttttcattac ttatccatcc ttattttaaa aaataaatac
    32941 aatattatta tagcagaaaa tgctaattac cccaataaaa ataagtgaaa catggggacg
    33001 tttcccctgt ctcacttcca gtgagacacg cgaaacgtcc ccatgtctcc
//
LOCUS       DAOOAB010000028        32814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1754_length_32814_cov_16.109619, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000028 DAOOAB010000000
VERSION     DAOOAB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 32814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32814
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_32814_cov_16.109619"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..654)
                     /locus_tag="PKU88_07445"
     CDS             complement(118..654)
                     /locus_tag="PKU88_07445"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPX69146.1"
                     /translation="METKNSNYSSTSLKIRDLVQISLLSALTFVAAWAIHIPYGNGGV
                     IHLGDSMIFLTAVVFGRRYAAISGAIGMTMFDIFSGFSVWAPYTFVIKAVMGLIAGSL
                     ANLGDSKGSSAVKNGLGMLAAGIWMIFGYYAAEAIITWNITQPLFSLLGNVIQAGAGA
                     AIAFVIIAAIKRSGYFNK"
     gene            complement(765..1412)
                     /locus_tag="PKU88_07450"
     CDS             complement(765..1412)
                     /locus_tag="PKU88_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69147.1"
                     /translation="MRKSADGKGNLKIADDSKTPSEIYSIVLRGVISTLRTIFLNYYV
                     SENIEALMRYINEKKIISSAEYKRLLYEAKDFYPVIVCALFINPLKKHTKNFKSVIID
                     NQSKKALFNEINEELNKFFNDWLCDVEIDPLLFFRLSAAYGKPFSLDLAIKQQLAAVL
                     TPIEERILKLQITEIVALYTIEQVATHLGLAGLDYIDYVKVEKKEEIVSIQSSRN"
     gene            1646..2392
                     /gene="rluF"
                     /locus_tag="PKU88_07455"
     CDS             1646..2392
                     /gene="rluF"
                     /locus_tag="PKU88_07455"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HPX69148.1"
                     /translation="MKNSDHDDSIRINKHISEKGLCSRREADELIRQGRVTINGKKAS
                     IGSKVYPGDIVKLGGKTLSPKAPPVYIAFNKPPGIVCTTDRREKDNIIDFINYPKRIF
                     PVGRLDKESEGLIFLTSDGDIVNKILRAGNNHEKEYVVTVDKPVTEDFIESMSKGVRI
                     LGTVTKKCRVSRISGYVFRIILTEGMNRQIRRMCEVFGYEVVKLRRVRIMNVNLGSLP
                     EGRWRYLTSHELNTINKLVAESVKTEEASR"
     gene            2489..4687
                     /locus_tag="PKU88_07460"
     CDS             2489..4687
                     /locus_tag="PKU88_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69149.1"
                     /translation="MKRMVSIITLAAVLLGILLAGPVAAAENGNLDLKEALEIAKIAF
                     DFDTANHDFSSSYSETKFGRKLWYLNWNSNDGRGSSMSVTVDAATGEIINMYQWKNMP
                     QPLNRIPKHTREEALEVAEALAGRLHPDKFKDTELMNEYWDNIYRSYYSSDSYNFNFI
                     RKIKGIDFQDNMIQVQVDKNTLEVRSFNLDWDTVIPIPDSAKAISADAAKKIFEEKLG
                     IELAYQMIYPNNSRDSKLILVYNQKNANHRIDAITGEIMTQPYYGLMEKSAMGGGDAG
                     AAGVAITPEEQKVIDDSSKYISKEKALESFTKLVPVDSRYKMDNSSLYGGYNNENAAW
                     SFSWSYNDSANNTYSYIYGTVDAVTGEVKSFSISNSENEYKAGRAQKYTKEQCREIAE
                     KFLKEIQPDKFSTSEYRELYFEIYADPQNVPSYNMNYIRKENGISVPFNNLSVVVNTY
                     TGEITNFSINWQDLDFPDTAGVISLEDAYKLLYAKHDLMLKYIRIYNYNRFEDNTEIK
                     LAYMPDDYYGMIDAKSGQFIDYNGNPVKEFKEISFTDIEGHKFENDIKLLVELGIIDS
                     AESKFNPDTRITQKEFVKLLMKATQPDYYPIPYAASDSSEYDRYYENAINQNILSRNE
                     KNPEACVTRMEASRMTVKALGVGFVADLGSIFNLNLKDAASITAENKGYAAIAAALGI
                     VDTSAGSFVPGLELTKAETAGMLISYLKVDKTPKSSEPTVGTPAIMPAAE"
     gene            complement(4818..5558)
                     /gene="fabG"
                     /locus_tag="PKU88_07465"
     CDS             complement(4818..5558)
                     /gene="fabG"
                     /locus_tag="PKU88_07465"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPX69150.1"
                     /translation="MRLKDKVAIVTGSTKGIGKETAMVFAWEGAKVVICGTRENAIKE
                     VTEEIKSMGAEALGFKVDVGNRAQVDEMVAEVKKVWGRIDILVNNAGITADAMLRKMT
                     EEQFDKVININLKGVYNCAQAVLDTMTEQGSGVILNASSIVGVYGNIGQTNYAAAKWG
                     VIGMTKTWAKELGRKGIRVNAVAPGFVMTPMLETVPEKVLDSLREKTPMQRLGKPEDI
                     ANAYLFLASDEASFITGTVLSVDGGLVI"
     gene            complement(5582..6757)
                     /locus_tag="PKU88_07470"
     CDS             complement(5582..6757)
                     /locus_tag="PKU88_07470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPX69151.1"
                     /translation="MREVVIVSGARTAIGNFNGSLAGVSAVDLGVTAVKEAIKRAGIT
                     PEMVDDTIMGCILQAGLGQGVARQVAVKSGIPVEKPAYTINMICGSGLRAVQVAAQQI
                     ISGECDIVVAGGTENMSAAPYLLRNARTGYRMGHGEIIDSMIYDSLTDVFNNYHMGIT
                     AENLAEQYNITREEQDAFAASSQNKAEAAIKEGKFKDEIVPVVIPSKKGDIVVDTDEF
                     PRAGVTAEALAKLRPAFKKDGTVTAGNASGINDGAAALVVMAADKAEELGIKPLARIA
                     GYASAGVDPSIMGIGPVKAVKKALERANLKLEDIDLIEANEAFAAQSIAVARDLGFDM
                     NKVNVNGGAIALGHPVGASGARILITLLYEMKKRNVKKGLATLCIGGGMGTALVVEM"
     gene            complement(7030..8412)
                     /locus_tag="PKU88_07475"
     CDS             complement(7030..8412)
                     /locus_tag="PKU88_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX69152.1"
                     /translation="MKKISYRLWVQMLLLIGITLGLLWVLQVVMLEQYYLYSQTRNIK
                     QEGIALAKAYEEGMNEELLTEKAEELNLKFNAGVEIYSINGERVFGFSLMPMMMGRAV
                     YKQEVFLEAAKGEAIVRQLKHNRLNSNILLVGIPIGEGPEIKGVMIVSAPLAAIKETK
                     EIIKEQLFIITVILILISLLLTYYSSKAFTKPILKINEAAAKMAKGNLSVRVDVESSD
                     ELGMLSDTINNLSAQLQKIEQLRKDLIANVSHEFRTPLSLIKGYAETIRDVTGDMKEK
                     RNSQLSIILEETDRLKSLVDDTLNLSQIQSEDFRPDIVPFDIFATVNSIMSRFAHMSE
                     EKGITLDMQWDKASVVVLGDEPRIEQVIYNFVNNAFNHTASGGWIKISISTNEDNVKI
                     EVSDNGHGIPSEELPYIWDRFYKASKDDKGKGLGLSIAKEIMEAHNSSYGVESIPEVG
                     TTFWFTLLKA"
     gene            complement(8409..9104)
                     /locus_tag="PKU88_07480"
     CDS             complement(8409..9104)
                     /locus_tag="PKU88_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX69153.1"
                     /translation="MSGVRILLVEDENRIREMVKEYLENEGFVIDEAADGAEALDKFG
                     NSEYDMLVLDVMIPKVDGWSVCREIRRASSIPILMLTARGEEYDKLFGFELGVDDYIV
                     KPFSPKELVARIKAVLRRSSNSGSNAYSAELFEYKGLLVNMSSRKVTIDERNIDMTPR
                     EFDLMSFFIKNRDRVFTREQLLNKVWGYEFGGDFRTVDTHIKMLRESLGEYRNLLVTV
                     WGVGYKFEAEDTK"
     gene            9318..9824
                     /locus_tag="PKU88_07485"
     CDS             9318..9824
                     /locus_tag="PKU88_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69154.1"
                     /translation="MKKALLATVLVLTLVLTSFSVFADGDQSILSKVTGLSDDEISEL
                     RTQRIGYGQLIPASVLSEMLGMDIKDVIGLRKAGKSYYQIAVDKGVTAGDYKKSLLEK
                     RNAYIDEQVKSGSITEDQGNLIKERMSVNIDGCTGQTPGAGRFGGGCGMGGGFGRGFD
                     RGFGRGSL"
     gene            complement(9891..10133)
                     /locus_tag="PKU88_07490"
     CDS             complement(9891..10133)
                     /locus_tag="PKU88_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69155.1"
                     /translation="MEYVKMPELLSAEAARVLAGNMDAVKEMMVAREQASYNGGCHDD
                     DICCPKKIKFIFVLNNVVIINVNAVAEEDNHGPSQC"
     gene            complement(10285..10785)
                     /locus_tag="PKU88_07495"
     CDS             complement(10285..10785)
                     /locus_tag="PKU88_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69156.1"
                     /translation="MDQVIMPELITPKFAEVIAENPEVLKAITDPQPGEQVIVGQNCS
                     MECGGNPTNVKLVFCNVTIGLDNASDDCNKGRCREKAEEHIEGCHIKIGDRGHEKGHD
                     DWGDISGRHKQIWMEMEILEARKNEIEKILKLIPRQIFNRKLVARCKREILEIEEQLS
                     AKRMEL"
     gene            complement(10877..11191)
                     /locus_tag="PKU88_07500"
     CDS             complement(10877..11191)
                     /locus_tag="PKU88_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69157.1"
                     /translation="MGYVKMPGMLSPELVKALAGNVHTVKEEPSPKDEMAAALKDFFK
                     ADKKERKYEDNECAQPKHKARRVIDDDEICCPKKVKFIFLLNNAVVINVNALAEENNT
                     PG"
     gene            complement(11337..11750)
                     /locus_tag="PKU88_07505"
     CDS             complement(11337..11750)
                     /locus_tag="PKU88_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HPX69158.1"
                     /translation="MKTIIKGKSLVGGSTEGTLIVSHEPFSFFGGFSFKTGSIVDTRS
                     DVYQEVLKGKIFAFPCGKGSSSTAGVLLEALRKGIAPRAIININSEKILAIGSIVAEK
                     MYGLNLPIISISEDDYQLLKTGLFLRINNEIIEVM"
     gene            complement(11778..13022)
                     /locus_tag="PKU88_07510"
     CDS             complement(11778..13022)
                     /locus_tag="PKU88_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HPX69159.1"
                     /translation="MKLTQDEQEILSGKRGEAAKIALEIIIELGELYGAAELIPVSKA
                     HIDGCLYGAVGEAGLEFAEKMHQMGGRVAVPTTLNANSRDINCWVNHRMNAEFAEKNA
                     RMEKAYLGMGAIPTWTCAPYQGVCAPRFGEQVAWAESNAIVYVNSVIGARTERYGDYT
                     DLCCALTGRAPKFGLHIKKNRLAQIIINCEGLPLKSSTDDFAVLGYLVGKLAKDKIPA
                     ITGIPQWVTSDQLKAFSAAIASSGAVAMFHMIGITPEAPDLNTISDDPSNIETIEITK
                     EMYLDSRKSLSSATPESADAILIGCPHASAAELMEIADLLNGRFIPEEMEFWIYTNRE
                     VYSLLESTLIIDNLIKSGVRIIRDTCFLNTDLSGWNMKTVMTNSAKYAHYMKALTGRE
                     VVFANLGECINTALQSKGQPRI"
     gene            complement(13350..14378)
                     /locus_tag="PKU88_07515"
     CDS             complement(13350..14378)
                     /locus_tag="PKU88_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69160.1"
                     /translation="MKTLKLCMIGFGNVGGEFCRLLEEKKAALLENNNCRVIITEIAT
                     KSRGILRNVNGIDTDGALDWIEKKGSFKGHEDYINLNSEEVIKSTTADALLELSTLSV
                     MDGQPAIKHIETAFDRNMHVITANKGPIAWRFKKLDTRAKEENVVFLYETVVMDGTPI
                     FNLVRNTLQGCRVLSFRGILNTTTNYILCEMEKGNSFEEAVKEAQRRGFAEADPSLDV
                     DGWDAAAKTAALMNVLMDAEVTPLDIKREGISGVIAADVLRVKQEGSCIKLICEGFFE
                     NGISVGRVYPKEIDESSIYSKIDATSSVLSVTTDLMGEISIVEHDPEIRQTAYGVYSD
                     LLALLGRI"
     gene            complement(14561..14794)
                     /locus_tag="PKU88_07520"
     CDS             complement(14561..14794)
                     /locus_tag="PKU88_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HPX69161.1"
                     /translation="MPYIAIKSFPKDQATKEAVAEKINEIFLEVWGCPQEAITISIED
                     ITPADWEEKIVKTEIDPKKENMMILSGKRCYKR"
     gene            complement(15101..17743)
                     /gene="ppdK"
                     /locus_tag="PKU88_07525"
     CDS             complement(15101..17743)
                     /gene="ppdK"
                     /locus_tag="PKU88_07525"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPX69162.1"
                     /translation="MKKYVYNFKEGKASMKDLLGGKGANLAEMSNIGLPVPPGFTITT
                     EACNEYYNKGEQLWSELIDEIMEKLTDLEATTGKTFGGGSNPLLVSVRSGAKFSMPGM
                     MDTILNLGLNDEVVKTVKDITDNERFAMDSYRRFIQMFSDVVMEIPKSKFERVLDEIK
                     EEKGAKYDTDLTASDLKEVVEKFKSIYKAEKGEGFPQNPKDQLLLAVTAVFRSWNNER
                     AIIYRAMNNIPGNLGTAVNVQSMVFGNMGNDCGTGVAFTRNPATGEKKIFGEFLMNAQ
                     GEDVVAGIRTPQTIEKLSEINQEVYDQFISVANLLENHYRDMQDIEFTIEKGKLFMLQ
                     TRNGKRTTAAALRVAVELVEEGKITKEEAVLRVEPAQLDQLLHPNFDSAALKKALSIA
                     KGLPASPGAASGKAYFTAADAVEASKNGEKVILVRLETSPEDIEGMAMAKGILTARGG
                     MTSHAAVVARGMGKCCVAGCEGLKVSEELKQFVANGKVYKEGDYISLNGSTGYVYEGV
                     IPTKEPEMSGHFATLMSWADEVRRLKVRTNADTPKDAEQAVKFGAEGIGLCRTEHMFF
                     SEDRIAAVREMIISETVEQRKVALAKLLPMQREDFIGIFEAMEERPVTIRLLDPPLHE
                     FLPQEDEDIKELAKTMDIEFGKLKARVESLHEVNPMLGHRGCRLAVTYPELAEMQARA
                     IIEAAVEVKKNKGIDVHPEIMIPLVGELKELEYIEKVVRQTADKVIAESGIKLEYLYG
                     TMIEVPRGAVTADEIAKTAEFFSFGTNDLTQMAYGFSRDDAGKFIEEYLKKGIFDQDP
                     FQTLDQNGVGKLVKMAVDLGRKTRPDIKLGICGEHGGDPASVEFCHRVGLNYVSCSPF
                     RVPIARLAAAQATIKDKMGEYSQK"
     gene            complement(17740..18558)
                     /locus_tag="PKU88_07530"
     CDS             complement(17740..18558)
                     /locus_tag="PKU88_07530"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HPX69163.1"
                     /translation="MNKNRAVIFVISDSIGETAEQVAKAASAQYPECEIRIRWIPYVT
                     EIESIQEVISEAKDLDSIIMFTLVVPELRKYLTEQAAMHNIICVDIMGPAVEAIAKVT
                     GQHPTDIPGAARKLDDEYFSKIEAVEFAVKYDDCKDPRGIKKADLVLLGISRTSKTPL
                     GMFLATKNLKVANIPLMPEVKPPKELFEISPKKIIGLVISTDQLNPIRLERLKSHGLS
                     DQANYASKERIAYEHKYAEEIMNKIGCRIVDVTDKAIEETANTILQILKEAEQE"
     gene            complement(18573..19214)
                     /locus_tag="PKU88_07535"
     CDS             complement(18573..19214)
                     /locus_tag="PKU88_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX69164.1"
                     /translation="MVAIEFTSRQKQIIELVKKYQPITSEQLAAKLNMTRAALRPDLT
                     ILTMSDMLEARPKVGYFYSGKIINDIGHDFIKSIKVAEIKSSAVVMDESATVYDSIVM
                     LFLRDAGTIYVQSKGALSGVVSRKDFIKIAIGNNDIQNMPIGMIMSRMPNIVYIFDND
                     SAYDAVVKLITHEIDSIPVVEPFKDSDGKEQFKITGKVSKTNMTKLLYDLYKQ"
     gene            complement(19345..21429)
                     /gene="glyS"
                     /locus_tag="PKU88_07540"
     CDS             complement(19345..21429)
                     /gene="glyS"
                     /locus_tag="PKU88_07540"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPX69165.1"
                     /translation="MSKDLLFEIGTEEIPANYMPSTLKQVKSISGAMLKNYRIAFEEI
                     KAYGTPRRIVLFVKGIAEQQENLEELVKGPSRRAAYDENGNPSKALLGFLKGRKAELG
                     DVFIQELSGVEYVYYKKQEKGQPVKKVLKTILPDILTSISFPKSMKWGNKSFRFARPV
                     RWLVPILGDELIKFDKDGIKCSRYTKGHRVLSKGSIKINNTAEYFDKLKAGFVIADQE
                     ERRAIIRRQCEELAREKGGEVLIDEELLEEVVYLVEYPTALIGGFDAEYLKLPKEAVI
                     TPMKEHQRYFPVVDNDKNLMNYFITVRNGDSRFLETVREGNEKVLKARLADADFFYSE
                     DRKESLDNCAEKLKNVVFQETLGTIYDKTQRISAIAEYLAAILGLNAGAKKNLLRSAH
                     LCKADLVTNMVKEFDELQGIMGREYALLQGEDREVADAIFEHYLPRFAGDCTPETLNG
                     SILGISDKIDTICGCFAIGIQPTGSQDPYALRRQAIGVTSIILDSNIHLGLAQLIHNS
                     LKPFEEKGILKGDEKSVKRDILEFFRQRLKNVMIDKGFEYDVIDAVINAGFDDIYDSY
                     LKILELSKWKYKDEFMNILGSFNRVSNLASKGKSTEINELLFTEKAELKLLHDYDKVY
                     AKFVNCVDNRDYGKALELMITLKKPIDDFFDNIMVMVEDEKIRNNRLGLLKSIGDMMN
                     RIADLSAVVMNK"
     gene            complement(21422..22318)
                     /gene="glyQ"
                     /locus_tag="PKU88_07545"
     CDS             complement(21422..22318)
                     /gene="glyQ"
                     /locus_tag="PKU88_07545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPX69166.1"
                     /translation="MTFQEIILTLQKYWADRHCIIAQPYDVEKGAGTMSPYTFLKALG
                     PEPWRIAYVEPSRRPADARYGENPNRLYQHHQFQVILKPSPDNVQDLYLGSLKALGID
                     PVKHDIRFVEDNWEAPTLGAWGLGWEVWLDGMEITQYTYFQQVGSIDCQLESAELTYG
                     LERIAMYIQDLSNVFDIEWVSGINYGDVFKTAEFEHSKYSFEEADTKLLFTLFDEYEA
                     EAKRLLDIGLILPAYDYVLKCSHAFNILDARKAISVTERTGFIGRVRNLARLVAQGYL
                     KQREELGYPLLKKAVFGGEKNE"
     gene            complement(22754..23311)
                     /locus_tag="PKU88_07550"
     CDS             complement(22754..23311)
                     /locus_tag="PKU88_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPX69167.1"
                     /translation="MDISLEKVDIIRDRTGVTYKEAKEALEAANGNVVDALINIEEAG
                     DKRWTETISVKGSEAVEKLKGIVKSGNVNRIRVKKDDYVILDIPVTAGAISAVVIPQL
                     TAIGTAVALLSKCVIEVERPNKQTINVSEAITNTADNLAGKIKNAADDFRNMTSSEAG
                     HNSAAADASDIFGAVNDNTDNTFKQ"
     gene            complement(23343..24119)
                     /gene="recO"
                     /locus_tag="PKU88_07555"
     CDS             complement(23343..24119)
                     /gene="recO"
                     /locus_tag="PKU88_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPX69168.1"
                     /translation="MPISKTEGIVLRYTNLGEADKILTILTRNNGKIKAIAKGCRKPK
                     SSLLSSSEVFVFSEFVLYKGTNFYHISQAVTRETFYNIRKDLLRLSYATYFAELAEAV
                     SDEGIPSERLFLLLAKTLYYLSTENIPIGILGVGYQLKLMDISGYRPNIARCAICRKS
                     SEDYTRFSVDLGGVICGSCSTGENQKNAGEVFRISQGTAEAINFLLNIEISRLNTKKI
                     DNTIFNEIEKITRSFIQSHLDKRFKSLDFLDSMKDLNAQK"
     gene            complement(24124..25026)
                     /gene="era"
                     /locus_tag="PKU88_07560"
     CDS             complement(24124..25026)
                     /gene="era"
                     /locus_tag="PKU88_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPX69169.1"
                     /translation="MNTKFKSGFVTIIGRPNVGKSTLINKLLGERVSIISSKPQTTRN
                     SIRCIYTTSEYQIVFIDTPGIHKPRHMLGEYMVKTATESLKGVDVVVVMFDCSAEVGP
                     GDMYAAQVAEKVNSPVILVLNKIDKIPKSRLEQIAASLIMHEDYFDSIIPVSASTGEN
                     LDELLKEIVKHLPEGPRYFPEDIYTDMPEKFIVSEIIREKVLELTSEEVPHGTAVEIT
                     SMKERENKDLMDIAATIYCEKESHKAIIIGKQGRMLKEIGVRSRVDIENLLHCRINLQ
                     LWVKVRRNWRDSRSALKNFGYNWE"
     gene            complement(25331..25711)
                     /gene="cdd"
                     /locus_tag="PKU88_07565"
     CDS             complement(25331..25711)
                     /gene="cdd"
                     /locus_tag="PKU88_07565"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPX69170.1"
                     /translation="MNYDVLIEKAQEAMENAYVKYSNFKVGAALLTRSGKVYTGCNVE
                     NSSFGATICAERVAFTKAISEGEREFEAIAVASSLKGFTYPCGICRQFMSEWGLDIKL
                     ITKSGDEVRVHTIGELLPEAFTEF"
     gene            complement(25717..26775)
                     /locus_tag="PKU88_07570"
     CDS             complement(25717..26775)
                     /locus_tag="PKU88_07570"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPX69171.1"
                     /translation="MKNNRCKFMLMILCIIAVIFSGCGKATEPVSSTEPEVTPEPVVE
                     PSPPPAPEPPKGSPCPLTGELVTYEADLKLRPIAVMIDNEYNARPQSGLSEAEIVYEI
                     PVEGNITRYMAIFHHTHADKVGPVRSARPYFIDKALEFNAVYVHCGGSPQALQDLVTL
                     KVNTLNDLKGSPCFWRSKDRKMPHNLYTSTKLMREVMETNKFNNKTAPEYFKFNEEFL
                     EPDGKATRSIIFNYSKNYVVGYEYSEKDKLYYRTINGTRLKDKESGKDIAAANIIVQK
                     TTRKVLDDVGRLEIKNIGKGRGYYLSGGKLIEIEWNKDGRSAKEVYKDLKGNEVLMNK
                     GVTWVQVVPDYVKVEIRE"
     gene            complement(26813..27295)
                     /gene="ybeY"
                     /locus_tag="PKU88_07575"
     CDS             complement(26813..27295)
                     /gene="ybeY"
                     /locus_tag="PKU88_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPX69172.1"
                     /translation="MSVLIDNRQEEIKVDEALEAFVVQVVEKVLAYEQCEEAFEVSIS
                     FVNNQEMRKLNKEYRNIDKETDVLSFPLAEFIEEELNTEDEGAEYIEEEIALGDIVIS
                     MEKAAEQSEEYGHSFKRELAFLLVHGMLHLLGYDHDEEASEGEIFDKQEEILKNMEIA
                     "
     gene            complement(27401..29467)
                     /locus_tag="PKU88_07580"
     CDS             complement(27401..29467)
                     /locus_tag="PKU88_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPX69173.1"
                     /translation="MIKYSVSDLKRLARNSTTIRALLVAGLFIALFLLFVLNTAPQKH
                     LLQVGDISDKDIYAPTDIVDKSATELRRKQAEDSVQPVYNLDLTVQIDIEKKISDFFM
                     LLNSVRSNAELDKVQKLNLIKQNSNISLGNSLYNMLLDVSEADLASLEHNIKYINNQM
                     LSGRITEQQLNDKRSDIREFFMNLPKKSQLNEIGADIAVMVLKPNMYYDEQTTLARKQ
                     SEREKVEDVVIRKGTLIVGKGQELSSQQIQLLNAYGLLAESDKDRFDIQFYLGYGVII
                     GICLIIMGLYLRSLDKKLWDSNSTLLLISLTAIMALTVTMMLKNISQYLIVLPAGAML
                     VSILVSPRIAIMTNIMTSILAGFLGGFDLHVVVMMLLTGILGAGMVSKTHHRSTFILS
                     GLAVSLVSLLVIFSEGILNREDIYRMINNGLYGVIGGTLSAVLTIGTLPFFETVFDII
                     TPLKLLELSNPNQPMLRRLLIEAPGTYYHSILVGNLAEAAAEDIGANALLCRVGAYYH
                     DIGKLKRPYFFKENQITRDNPHDKITPNLSALVITSHVKDGVEIAGKNKLPSTVIRII
                     EEHHGNTLVAYFYHKALSAEGAEAVDESKFRYPFRKPQTEEAAIVMLADSVEAYIRSL
                     SEPTRDQVEQGVRKIIKDKLNDAQLDNCDLTLKDLEMIGQAFVKVLAGIFHDRIEYPE
                     TIKDVQ"
     gene            complement(29464..30399)
                     /locus_tag="PKU88_07585"
     CDS             complement(29464..30399)
                     /locus_tag="PKU88_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPX69174.1"
                     /translation="MERIMNLFGNFDQNLKVIEDEFNIDIIVREGEVKIVGQENAVAM
                     AERTLNTLMKIQENGEAVTSHAVSYTIELVKEGRENRSEEFDSVIITNARGKAIKSKT
                     IGQKRYIDAIRNNEVVFGIGPAGTGKTYLAMAMAVKAFKSHEVSRIILTRPAVEAGEK
                     LGFLPGDLQNKVDPYLRPLYDALHDIMGAETYQKHIERGVIEVAPLAYMRGRTLDDSY
                     IILDEAQNTSPEQMKMFLTRLGFGSKAIITGDITQIDLPKGKYSGLKQVMEILKGIKG
                     IGFVYFSEKDVVRHKLVKDIIKAYEKHEEEIGESE"
     assembly_gap    30495..30594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30639..31817)
                     /gene="yqfD"
                     /locus_tag="PKU88_07590"
     CDS             complement(30639..31817)
                     /gene="yqfD"
                     /locus_tag="PKU88_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HPX69175.1"
                     /translation="MFIIRIWNYLRGYAIIIVKGLKIERFINLAVVNNIYIWDIEKLD
                     YTTVKAKIGLENFGRLRDIVRKTDSSVSILNKRGFPFILRNIRRRKLFYSGLFLMCVF
                     IYIMSSFVWMIEIVGTKDVDNESILRCLYNEGLKEGSWKGRLDRRHIENQVLIKMPEL
                     SWIGIQIKGTKAVVEVVEKSEGPPLISRTDACDIVAAKDGVITKLLVLSGDPLVKDGD
                     SVRKGQKLVTGTILRENLEPRQIHSLAQVSARTWYEDAEEIPLQQIEFKPTGRVARQY
                     KLKLLDKEFSRKKSVNFKDFNEYVEERHLISFGDYVFPIKLIEYRFEELVPELKTIMV
                     DEAKERCAERLNARIKLRIPEDAVILKRDINYYTDKKSVKAKISVEVLEDIGVKQKIY
                     "
     gene            complement(31850..32122)
                     /gene="yqfC"
                     /locus_tag="PKU88_07595"
     CDS             complement(31850..32122)
                     /gene="yqfC"
                     /locus_tag="PKU88_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HPX69176.1"
                     /translation="MGKRSYKLREKLSNTFELPKDIVLDVSKIIIIGTGQITVENHKG
                     IIEYSEELIRINTGSGIVALHGKNLAIKTIFQEEITITGEITNMEF"
     gene            complement(32271..32771)
                     /locus_tag="PKU88_07600"
     CDS             complement(32271..32771)
                     /locus_tag="PKU88_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69177.1"
                     /translation="MEGIYIVIAIIIALFNLAAKQQKNQGRGRRGMNRQQGQYRQKPV
                     QRQKHIPQWSEGPFADLGKSWNELFGSPEEHVPESKPAEDYEGMEIEDRRRVGSLDYV
                     EHSSSSEGICDEHPEHSRLDALKAEIRETEEEGFIFEIAEDNLLRSVVMAEVLGPPRA
                     MKRRIR"
BASE COUNT         8420 a         8136 c         5899 g        10259 t
ORIGIN      
        1 agacataggg acacggggac gcttcgggac acggggacgt ttctcctgtc ccgctttcag
       61 cgggacagga gaaacgtccc cgtgtcccga agcgtccccg tgtcttgcca gaacctgtta
      121 tttattgaaa tagccgctcc tttttatggc tgctattatt acaaaggcta ttgccgcacc
      181 ggcaccggcc tgtatgacat tgcccaatag gctgaataac ggctgtgtga tattccatgt
      241 tataattgct tccgcagcat aatatccgaa tatcatccaa attcctgccg ccagcattcc
      301 caatccattc ttaaccgcac tgctgccctt tgaatctcca aggttcgcca ggctgccggc
      361 aatcaaaccc attaccgctt taatcacaaa agtatatggt gcccatacgg agaaacccga
      421 aaatatatca aacatagtca tacctattgc tccggatatt gcagcatatc ttctgccaaa
      481 gaccaccgcc gtaaggaata tcatactgtc tcccagatgt atgactccgc cgtttccgta
      541 gggtatgtga atagcccagg cagctacaaa agtcaatgcg gataaaaggc ttatctgtac
      601 taaatctctt attttcaaac tggtgctgct gtaatttgaa ttttttgttt ccattttgcc
      661 gactccccca aaattatata ttgctgttgc tgcttctctg atttcattat acaggagccg
      721 ccggagggaa caatacggaa tcagcaattt tatgcacagt ttatttagtt tctgcttgat
      781 tgtatcgata caatctcttc tttcttttcc acctttacat aatcaatata atcaagccct
      841 gcaaggccca aatgtgttgc aacctgctct atggtataca aagcaacgat ttctgttatt
      901 tggagcttta gaatcctttc ttctattggt gtaagcacgg ctgcaagctg ttgttttatt
      961 gcaagatcaa ggctgaaagg ttttccatag gctgcggaaa gccgaaaaaa cagcagcgga
     1021 tctatctcca catcacagag ccaatcattg aaaaatttat ttaattcttc attaatctca
     1081 ttgaacagcg cctttttgga ctgattatca ataattacgc tcttgaaatt cttcgtatgc
     1141 ttttttaaag gattaatgaa caatgcgcaa acaatgacag gataaaaatc ctttgcctca
     1201 tataaaagcc ttttatactc tgcagagctt attatcttct tttcattaat atatctcatc
     1261 agtgcctcga tattctccga gacataatag ttcaaaaata ttgtcctcaa ggtactgata
     1321 acgccccgca atacaatgct gtaaatctct gaaggagtct tactgtcgtc tgcaattttc
     1381 agatttcctt tgccgtctgc actttttctc atgttcacac ctccacaaga ctttcaagga
     1441 aatttaatga acccctgagc cctttttcct ctatatctaa ttatttacaa attcttttta
     1501 tctattcagc aatttgatat acattaattg attaatttac cgtatataag taaaatataa
     1561 atacaggcac ggagctgcgg ttcagggata cccggtgatt gccccctggt ggctgccgtg
     1621 caaaaagttg aaagggcatt ttattatgaa aaactcagat catgatgata gtatccgaat
     1681 caacaaacac ataagcgaga agggcttgtg ctcccgcagg gaggctgatg agcttatcag
     1741 gcagggaagg gtcacaataa acggcaagaa ggcttccatt ggcagcaagg tatatcccgg
     1801 ggatattgta aagctcggag gaaaaaccct ctctcccaaa gctcctccgg tctacattgc
     1861 ctttaataaa cctcccggaa ttgtatgtac tacggacaga agggaaaaag acaatattat
     1921 agactttatc aactacccta aaagaatatt ccccgtagga aggcttgata aagaatcgga
     1981 aggcctgatt ttcctcacaa gtgacggtga tattgtaaat aagatactcc gggcaggcaa
     2041 caatcacgag aaagaatacg tagtgacggt agacaaacct gttactgaag attttatcga
     2101 atccatgtca aagggtgtac ggattctggg aactgtaaca aaaaagtgca gggtatcccg
     2161 gatatccggg tatgtgttca ggataatact tacggaagga atgaacaggc agatcagaag
     2221 gatgtgtgag gtgttcggat atgaagttgt gaagctgagg cgggtacgga ttatgaacgt
     2281 taatctgggt tcacttccgg aaggaagatg gagatatctt acaagccacg agcttaatac
     2341 aattaacaag cttgttgcag aatcagtgaa gaccgaagag gcctccagat aaatgttcct
     2401 ccggaatcat cgttttgccg gaacttttat taattattgg cagtcaaagt tgtatacatt
     2461 caaataagct atgaaaggga gataaattat gaaaaggatg gtatccatta ttacactggc
     2521 tgcagtactc ttaggcatcc tgcttgcagg gccggtagcc gctgcagaga atggtaatct
     2581 ggatcttaaa gaggcattgg aaattgccaa aatagctttt gactttgata ccgcaaatca
     2641 tgacttcagc agcagctatt cagaaacaaa attcggaaga aagctgtggt atctgaactg
     2701 gaattccaat gatggaagag gctcaagcat gagtgttacc gttgatgcgg caacagggga
     2761 aataataaac atgtaccaat ggaagaacat gccgcagcct ttaaacagaa ttccaaaaca
     2821 caccagggaa gaagctttgg aggtagccga ggctctggcc ggaaggcttc atcctgacaa
     2881 attcaaagat acggagctta tgaacgaata ctgggacaat atctatcgat cctattacag
     2941 cagcgattca tacaacttca atttcataag aaagataaag ggaatagact ttcaggataa
     3001 tatgatacag gtacaggtag acaaaaacac cctcgaggta agaagcttca acctggattg
     3061 ggatacggta atacctattc ccgactctgc aaaagcaata agcgcggatg cggcgaagaa
     3121 gatatttgaa gaaaagctcg gtattgagct ggcatatcag atgatatatc cgaacaattc
     3181 aagggattcc aaactgatac tggtatacaa tcagaagaat gcaaaccatc gtattgatgc
     3241 cattaccggc gagataatga cccagcctta ttacggatta atggaaaagt ccgccatggg
     3301 cggcggcgat gccggtgcgg caggtgttgc gattactccc gaggagcaga aggtgattga
     3361 cgattccagc aagtatataa gcaaggaaaa agccctggaa tcttttacaa aacttgtacc
     3421 tgtcgacagc aggtataaaa tggataactc aagcctctac ggcggttata acaatgaaaa
     3481 cgcagcctgg agcttcagct ggagctataa tgacagtgcg aacaatactt acagctatat
     3541 atacggcacg gtagatgctg ttaccggtga agtcaaaagc tttagcatca gcaacagcga
     3601 gaatgaatac aaagccggac gcgctcaaaa gtatacaaag gaacaatgca gggaaatagc
     3661 ggaaaaattc cttaaagaaa tacagcctga caaattcagc acctctgaat acagggagct
     3721 gtactttgaa atatatgcag atcctcagaa tgtacccagc tacaatatga actatatcag
     3781 gaaggagaac ggcatctctg ttcccttcaa caatttgagc gtagtggtaa acacatatac
     3841 cggagaaata acaaacttct ccataaactg gcaggacctg gacttccctg acacagcggg
     3901 tgtaataagc ctggaagacg cttataagct gctgtatgca aagcatgacc ttatgcttaa
     3961 gtatataagg atatataatt ataacaggtt tgaagacaat acggaaataa agcttgctta
     4021 catgccggat gactattacg gcatgattga tgccaagagc ggccagttta tagactataa
     4081 cggaaatccg gtaaaagaat tcaaggagat atccttcacc gacatagaag ggcataaatt
     4141 tgaaaacgat atcaagcttc ttgtggagct gggaattatt gattccgccg aaagcaagtt
     4201 caatccggat acaagaataa cccagaaaga atttgttaag cttctgatga aagcaaccca
     4261 gccggattac tatcctatcc cctatgcagc aagcgacagc agcgaatacg acaggtacta
     4321 tgaaaatgcc ataaatcaga acatactgtc cagaaatgaa aaaaatcccg aagcctgcgt
     4381 cacaagaatg gaagcgtcaa gaatgacagt aaaagcattg ggtgtaggct ttgttgcgga
     4441 cttgggaagt atattcaacc tcaatctcaa ggatgccgca agtattaccg cagaaaacaa
     4501 aggctatgcc gccattgcag cagctttagg aatcgttgac acttctgcag gcagcttcgt
     4561 accgggtctg gagcttacaa aagctgaaac agcagggatg ctcataagct atcttaaggt
     4621 ggataagaca ccaaaatcat cggaaccaac cgtagggaca ccagcaataa tgccggcggc
     4681 agaataaagc cgaaaagaag gacacgggga cgtttctcct gtcccggaag gacacgggga
     4741 cgtttctcct gtcccgctga aagcgggaca ggagaaacgt ccccgtgtcc ttcttttcgt
     4801 gtcctttttt atgtaaatta gattacaagc cctccgtcta ccgaaagtac agttcctgtt
     4861 ataaagcttg cttcatctga tgccaggaac agatatgcat ttgcaatatc ctccggttta
     4921 ccaagcctct gcatgggggt tttttccctg agtgaatcca gaaccttttc aggaacagtc
     4981 tcaagcattg gagtcattac aaagccgggg gcaacggcat taacccttat accctttctt
     5041 ccaagctcct tggcccaggt ctttgtcata cctataactc cccacttggc agctgcatag
     5101 tttgtctggc ctatgttccc atatacccct actatggagg aagcattgag tattacgccg
     5161 cttccctgct ccgtcattgt gtcaagtaca gcctgagcac agttgtatac gcccttcagg
     5221 ttgatattga taactttgtc gaactgctct tccgtcatct tcctaagcat tgcatccgct
     5281 gttatgcccg cattgttaac aagtatgtct attctgcccc aaaccttctt aacttccgca
     5341 accatttcat caacctgtgc cctgttgcct acgtctacct tgaagccaag agcctctgct
     5401 cccattgact taatctcttc tgtgacttct ttaattgcgt tttcgcgtgt accgcagatt
     5461 accacctttg cgccttccca ggcaaagacc attgcggtct ccttgcctat cccttttgta
     5521 gaacctgtga ctatggctac tttatcctta agcctcatat actttcctcc ttttttgtat
     5581 actacatttc cactacaagt gcggtaccca tacctccgcc tatacataag gttgcaagtc
     5641 cttttttcac gtttcttttc ttcatctcat aaagaagtgt tataagtatc cttgcgccgc
     5701 tggctcccac cggatgtcca agtgcaattg cgcctccgtt gacattcacc ttgttcatgt
     5761 caaaacccaa atcccttgcc actgcaatag actgagctgc aaaagcctca ttggcttcga
     5821 taaggtctat atcctccagc ttgaggttcg ctctttccaa agctttcttt actgccttaa
     5881 ccggccctat acccattatg cttggatcca ctccggcaga tgcataacct gctattctgg
     5941 caagaggctt tatgccgagt tcttccgctt tatccgcagc cattacaacc aatgccgcgg
     6001 caccgtcatt tatacctgaa gcatttccgg ctgtaacagt accatccttt ttaaacgcag
     6061 gcctcagctt cgccaaggct tctgcagtaa ctcccgctct tgggaattcg tcggtatcaa
     6121 ccacaatatc accctttttt gaaggaatga ccaccggaac tatctcgtcc ttgaatttgc
     6181 cttccttaat agctgcttcg gctttgttct ggctggaagc agcaaaagca tcctgctctt
     6241 ctctggttat attgtattgt tccgccaggt tttcggcagt aatgcccata tggtaattgt
     6301 taaatacatc agtgagggag tcgtatatca ttgaatctat tatttctccg tgacccattc
     6361 tgtagcccgt tctggcattt ctgagcaaat aaggagctgc gctcatgttc tcagttccgc
     6421 ctgcaacaac aatgtcgcac tctccggata ttatctgctg ggcagcaacc tgtacagccc
     6481 tcagacccga accgcatatc atgtttattg tataagcagg cttttcaacc ggtatccctg
     6541 acttcacagc tacctgacgt gccactccct ggccaaggcc tgcctgaaga atacatccca
     6601 tgatggtatc atccaccatt tcaggagtga tccctgctct tttaattgcc tccttgactg
     6661 cagtcacccc aaggtctact gcggaaactc ccgccaggct gccgttgaaa ttgcctattg
     6721 ctgttcttgc cccacttacg ataacaactt ctctcatcgt gtttaatacc tcctcaaaga
     6781 attaattact attcgtataa aagcaatgca acttttatgc caaattccct tcagccctat
     6841 gtgacataga aaacgagcaa cacggaacat ttatatattg tgcagctgca tcagaaaact
     6901 tcgataatca cttcattgcc gttcattatt catgattcaa aaattatcgc cctccgtgca
     6961 ttcagtggtt ttctgtgttt tcagtgttat ccgtatctag ttcccttttc gcctaaaatt
     7021 gggcatatat tacgctttca aaagagtaaa ccaaaatgtt gttcccactt ccggtatact
     7081 ttctactcca tagctgctat tatgtgcttc catgatttct ttggctattg aaagccccag
     7141 tccttttcct ttgtcatcct tggatgcctt atagaatcta tcccaaatat atggaagttc
     7201 ttctgatggt atgccatgac cgttatctga tacttcaatt tttacgttat cttcattcgt
     7261 cgaaattgaa atttttatcc agcctccgga agctgtatga ttgaatgcat tatttacaaa
     7321 attgtatatt acctgctcaa tacgcggctc gtctcccaac acaacaacgc tggccttatc
     7381 ccactgcata tccagggtta tccccttttc ctccgacata tgtgcaaacc tgctcataat
     7441 gctgtttact gtcgcaaaaa tatcaaaagg aactatgtcc ggcctaaaat cctccgactg
     7501 aatttgggac agattcaaag tatcatctac cagactcttt aacctgtcag tctcttccaa
     7561 tataatgctt aactgactgt ttcttttttc cttcatatct cccgtaacat cccttattgt
     7621 ttctgcatat cccttgatca ggctaagggg agtcctgaat tcgtgggaca catttgctat
     7681 aagatccttc ctcagctgct caattttctg aagctgcgct gaaaggttat ttattgtatc
     7741 tgaaagcatc ccaagctcat ctgagctttc tacgtcaacc ctgacggata gattgccttt
     7801 agccattttt gctgcagctt cattgatttt cagtataggt tttgtaaagg cttttgatga
     7861 atagtatgta agcagcagag atatcagaat aagtatgact gtaattataa atagctgctc
     7921 cttaataatt tccttagtct ctttaatcgc agcaaggggc gcactgacaa tcatcactcc
     7981 ctttatctca ggcccttctc ctatcgggat tccgaccagc aaaatattgc tgttgagcct
     8041 gttgtgcttt agctgtctga ctattgcttc acctttagca gcttccaaaa atacttcctg
     8101 cttatataca gccctgccca tcatcattgg catgagagaa aatccaaata cccgctctcc
     8161 atttatggag tatatttcta cgccagcatt aaacttaaga tttaattcct cagccttttc
     8221 tgtaagcagt tcttcattca ttccttcttc ataagccttt gccagtgcaa ttccctcctg
     8281 cttgatattt ctggtctggc tgtacaagta gtactgttcc agcataacaa cctgaagaac
     8341 ccaaagtaat ccgagggtaa tcccaataag cagcagcatc tgaacccata acctgtaaga
     8401 tatctttttc attttgtatc ctccgcctca aacttatacc ctactcccca gaccgttacc
     8461 aaaaggttcc tgtactctcc caggctctct ctcagcattt tgatatgggt atctaccgtc
     8521 ctgaaatctc ccccgaactc atagccccat actttgttca aaagctgctc tcttgtaaaa
     8581 accctatccc tgttttttat aaaaaaactc atcaggtcaa actctcttgg tgtcatgtct
     8641 atattccttt catctattgt aacctttctg gaactcatat tcaccaaaag ccctttatat
     8701 tcgaacagct cagcggaata agcgttactc ccggaattac tgctgcgtct cagtactgcc
     8761 ttaattctgg caacaagttc cttagggctg aatggtttta caatgtaatc atcaacacca
     8821 agttcaaatc caaacagctt gtcatattct tcccctctgg ctgtcagcat caatataggt
     8881 atggaggagg cgcgtcttat ttccctgcat acgctccagc catccacctt gggaatcatt
     8941 acatcaagca ccagcatatc atattctgaa tttccgaact tatccagggc ttctgccccg
     9001 tctgcggctt catcaataac gaacccctca ttctccaggt attcctttac catttccctg
     9061 attctattct catcctccac cagaagaatc cttacacctg acataacagc accaccttac
     9121 ttggtttgta tcccactccg gtattattta atgatattat tacacaaatt gacattcaat
     9181 gtcactacat aatcctgtgg tttttcaatt ttcataaatc caacacaatt tcatcattat
     9241 atagacaaat ctataatgta gaataaaatc aaggcaataa tatgaagcct gaaactatta
     9301 aagaaagggt gaaaaaaatg aaaaaagcgc tattagctac tgtattggtt cttacgttgg
     9361 tattaacatc attttcagtt tttgcagatg gtgatcaaag catactatca aaagtcacag
     9421 gattgagcga tgatgaaata tcagagctga gaactcagag aatcggttat ggacagctta
     9481 taccggcatc tgtgctttca gaaatgcttg gcatggacat taaagatgta ataggactga
     9541 ggaaggcagg aaagtcatac tatcagatag ctgttgacaa aggagtaact gccggggatt
     9601 acaaaaaaag cctgcttgaa aagagaaatg cttatattga tgagcaggtt aagtcgggaa
     9661 gcatcacaga agatcagggc aacctcataa aggaaagaat gagcgtaaac attgacggct
     9721 gtaccggtca gactccaggg gcaggcagat tcggcggtgg atgcggtatg ggcggaggct
     9781 tcggcagagg cttcgacaga ggattcggcc gcggctccct ataacttcaa aaaggataaa
     9841 tggcccttgc catttatcct ttttgtggtt ctgaacttat ctgacacagc ttagcattgg
     9901 gaaggaccgt gattgtcttc ctcagccact gcattaacat tgattatcac aacattgttc
     9961 aaaacaaata tgaacttaat ctttttgggg cagcagatat catcgtcatg acagccaccg
    10021 ttatagcttg cttgttccct tgcaaccatc atttctttta cagcatccat atttccggca
    10081 agcacccttg ctgcttcagc tgacagtaat tccggcatct ttacatattc cataaattca
    10141 cctcctttca ttttctgggc ggagaatttg aaccggttcg tcttcttcca atagcattat
    10201 atgacttgta cattttattg ctactgattg tacaataaac tctatcaaca ttttctcaca
    10261 atatcccata ctccataaac caagttacag ctccatcctt ttagctgaca attgctcctc
    10321 tatttcgagt atctctcttt tacaccgggc aaccagtttc ctgttaaata tctgtcgggg
    10381 tatcagtttc aggatttttt caatttcgtt ttttcttgct tcaagtattt ccatctccat
    10441 ccatatctgt ttatgtctgc ctgaaatgtc tccccagtcg tcatgccctt tctcatggcc
    10501 cctgtcacct atctttatat gacagccttc aatatgctct tccgcctttt ccctgcagcg
    10561 gcctttattg caatcatctg atgcattatc cagaccaata gtaacgttgc aaaacacaag
    10621 cttgacattc gtcggattac ctccgcattc catactacag ttctgcccga cgataacctg
    10681 ctcccccggc tgaggatcgg taatcgcttt cagcacttca ggattttcag ctatcacttc
    10741 tgcaaacttc ggtgttatta attcaggcat aataacttga tccatgtttt catcacctcc
    10801 cttgctggat tctgctatgt atagtaaagc ataggtacaa taattgaatt atgtacctat
    10861 gcatttcata ttatctttag cccggagtgt tattttcttc agccaatgcg ttgacattaa
    10921 tcactactgc attattcaac agaaatatga acttgacttt tttaggacag cagatttcat
    10981 catcgtctat tacccttctt gctttatgct ttggctgtgc acattcattg tcttcatatt
    11041 ttctttcctt cttgtctgct ttgaaaaaat ctttcaaggc tgccgccatt tcatctttgg
    11101 ggcttggttc ttctttaaca gtgtgcacat tgcctgccaa tgcttttacc aattcaggcg
    11161 aaagcatacc cggcattttt acatatccca tggattcacc tcccctcatt aaattgacag
    11221 agattcagaa ccggtttgtc ttcttccaat agcattatat gacttgtaca ttctattgct
    11281 acaattcata aaaaaaaacg cctcacggcg tcaaaacttg tattgtttgc cttgtttcac
    11341 attacttcga tgatttcgtt attaatacgg aggaataaac ctgttttcag cagctgataa
    11401 tcatcttcag aaatacttat gatgggcaga ttcaatccat acattttctc tgctacaata
    11461 gaaccaatgg ctaaaatttt ttcactatta atattaatta ttgcccgggg ggcgatccct
    11521 tttcttaaag cctcaagaag cacaccggct gtactgcttg atccctttcc acagggaaag
    11581 gcaaaaattt tgccttttaa tacctcttga taaacatcgc ttctggtatc tacaatactg
    11641 cctgtcttaa aactaaaacc gccgaaaaag ctaaagggtt catgggaaac aatgagagtt
    11701 ccttccgtac tgcctcccac aaggcttttt cctttaataa ttgttttcat ttcatttctc
    11761 ccgtataatt attttaatca gatccggggc tgacccttgg attgaagagc cgtattaata
    11821 cattcaccca aatttgcaaa tacaacctcc ctaccggtta atgccttcat ataatgggca
    11881 tatttggctg agtttgtcat aactgttttc atattccaac cgctaagatc agtatttaaa
    11941 aagcaggtat cccggataat tctcacaccg ctttttatca aattatcgat aataagggtt
    12001 gattccaaca gggaatatac ttctctattg gtataaatcc aaaattccat ctcttcagga
    12061 ataaacctgc cattaagtaa atctgcaatt tccattagct ccgctgcgga agcatgaggg
    12121 cacccaatta aaatggcatc tgcactttcg ggagtcgcgc tgcttaaaga tttcctggaa
    12181 tctaaataca tttcttttgt aatctctata gtttcaatat tggaaggatc atctgatatt
    12241 gtatttaaat ccggtgcttc cggcgtgatc ccaatcatat gaaacatagc tactgcccct
    12301 gatgaagcaa tagccgcgga aaatgctttc agctgatctg atgttaccca ttgtggaatt
    12361 ccggttatag cggggatttt atccttggca agcttaccca caagataccc caatacagca
    12421 aagtcatcag tgcttgattt taagggtaac ccctcacaat ttataatgat ctgagccagg
    12481 cggtttttct tgatatgtag tccaaatttg ggtgcccggc cggttaatgc acaacaaagg
    12541 tcggtgtaat ctccatatcg ttcggttctg gcaccaatga cagaatttac atatactatg
    12601 gcattcgatt ctgcccaagc cacttgctcc ccgaatcgag gtgcacaaac cccttggtaa
    12661 ggagcgcaag tccaggtagg gatagcgccc attcctaaat aagctttttc catcctggca
    12721 tttttttctg caaactctgc attcattcta tggtttaccc agcaattgat gtccctggaa
    12781 ttggcattaa gggttgtagg taccgcgaca cgccccccca tttgatgcat tttttctgca
    12841 aactccaagc cagcctcacc aacggcgcca taaaggcagc cgtcaatatg cgccttagaa
    12901 acaggaatca gttccgcagc cccatacaat tcacccaact caatgatgat ttcaagagca
    12961 atttttgctg cttcgcctct cttaccggat aaaatttcct gttcatcttg cgttaatttc
    13021 aaatttttca cctctgttaa taattctatc atcattatta ttggtctttt cattggtctt
    13081 ttcgctattc tctatttctt tccctcattc tagtaattat taacacaaca actttctatt
    13141 ctttcatagt gttaataatc accacattga ttcagtgcct cgcatgctca agaagggata
    13201 tacgttacga ggcactagta tgtatgttcc aacaaaggtc ttgccgttaa cttcgggaga
    13261 acagttattt ccggtaaaga agtgggcata tggtgtggtg ccaggcacct attacttatt
    13321 gaacagcgcc tttcatcgct ccttattttt tatatcctgc caagtaatgc aagcaggtcg
    13381 ctgtatacac catatgcagt ctgccgtatc tccggatcat gctccacgat acttatttca
    13441 cccatcagat ccgtagttac cgacagtacc gatgaggttg catctatctt gctgtatatg
    13501 ctgctttcat caatctcctt tggatatacc cgtccaactg agataccatt ctcaaaaaag
    13561 ccttcacata taagctttat gcagctcccc tcctgcttta ctctcagcac atctgctgct
    13621 atgacgcctg atatgccttc cctctttata tcaagaggcg tcacctctgc atccatcagc
    13681 acattcatga gagcagcagt tttggcagcc gcatcccagc catccacatc aaggctgggg
    13741 tctgcttccg caaagcctct cctctgcgct tccttcacag cttcttcaaa gctgttgcct
    13801 ttttccatct cgcaaaggat atagttcgtt gtggtattga gtatgcccct aaaggatagc
    13861 accctgcagc cctgcaatgt attcctgact agattgaata ttggagtacc atccatgaca
    13921 acagtctcgt acaaaaatac cacattttcc tcttttgccc tggtatcaag ctttttgaag
    13981 cgccatgcta taggcccttt atttgcagtt attacatgca tatttctatc aaaagcagtt
    14041 tcaatatgct ttatggcagg ctgaccgtcc attaccgaca aagtgctgag ttccagcagc
    14101 gcatcagcag tagtactttt tattacttct tcagaattca agttgatata atcctcatgt
    14161 cctttaaagc tgcctttttt ctctatccag tccaatgctc cgtcagtatc gataccattt
    14221 acatttctta atattcctct gctttttgtt gcaatttcgg tgataatgac tctgcaatta
    14281 ttattttcca acagcgccgc cttcttttcc tccagcagcc tgcagaactc tcccccgaca
    14341 ttgccgaagc ctatcatgca tagcttaagt gttttcatat acagcgctct cctccttact
    14401 ctattatatt tcatacagca gctattcaca atcttcaccc tggcacctat tattttaaga
    14461 gccttcatcc tgtgttgatt atctgttttt tcacattatg gaacacttct ggagaagcta
    14521 ctttacaaat tggaatttgt aagcacgtga agtcaaatca tcaccgttta tagcatcttt
    14581 taccagacaa gatcatcatg ttttccttct taggatcaat ctcagtcttg acaatcttct
    14641 cctcccaatc cgccggtgtg atatcttcaa ttgaaatggt gattgcttcc tgcgggcagc
    14701 cccagacctc caaaaaaatc tcgttgattt tctcggcaac cgcttccttc gtcgcctgat
    14761 ccttggggaa tgacttaatc gcaatataag gcatactaaa atctcctttc aaatttaatg
    14821 aagcagcggg gaagcagcgg ggacggttct ttctgctttt ctttctgctt tttcaaatat
    14881 catattacct tgtttgaata aagaaaagta aaaggaaccg tcccttttgc tttcccatgg
    14941 gcattactac agtattcttg ccctggtttg cttgagtctt agcatgaaat atgctgaaac
    15001 accgacatca ttataccgca tttaatcaag cttttcaaaa aaaaacaagg gaagccatgt
    15061 cagcctccct tgtcatatac actggcttta tgaatttctc ttacttctgg ctgtattcac
    15121 ccattttatc cttaatggtt gcctgtgctg ctgctaacct tgctatcggc actctgaaag
    15181 gtgaacagga tacataatta agcccaaccc tatggcagaa ttcaacggat gcagggtccc
    15241 cgccgtgctc accgcagata cccagcttaa tatccggcct ggtcttcctg ccaaggtcta
    15301 ccgccatctt taccagcttt ccaactccat tctgatcgag agtctggaat ggatcctggt
    15361 cgaatatgcc tttttttaga tactcctcta tgaacttgcc tgcatcgtcc cttgagaagc
    15421 cataggccat ctgtgtaagg tcatttgtac cgaaggagaa gaattcggct gtctttgcta
    15481 tttcatcagc agtcacagct cctctgggta cctcaatcat tgttccgtac agatactcaa
    15541 gcttgattcc agattcggct ataaccttat cggcagtctg ccttacaacc ttctcaatat
    15601 actcaagctc tttcagttca cctaccaaag gtatcattat ttcaggatgg acatctatgc
    15661 ccttattctt tttaacctca actgcagctt caattattgc ccttgcctgc atttcagcca
    15721 gttcgggata tgttaccgcc aatcggcagc ccctgtgccc cagcatcggg ttgacttcat
    15781 gaaggctttc aacccttgct ttaagctttc caaattcgat atccattgtc tttgccagtt
    15841 ctttgatatc ttcgtcttcc tggggaagga actcatggag cggcggatcc aaaagcctta
    15901 tggtaacagg cctttcttcc attgcttcaa atattccgat gaaatcctct ctctgcattg
    15961 gaagaagctt tgccaatgcg acttttcttt gctccactgt ctcggatata atcatttctc
    16021 ttaccgctgc tattctgtct tcactgaaga acatatgctc agtacggcaa aggccgattc
    16081 cttctgctcc gaatttcaca gcctgctccg catccttcgg tgtatctgca ttagtcctca
    16141 ccttaagcct tcttacttca tcagcccagg acataagagt tgcgaaatgt cctgacattt
    16201 ccggctcttt tgtcgggata accccctcat agacataacc ggtgctgccg tttaaggata
    16261 tgtagtctcc ttctttgtat accttgccgt tagccacaaa ctgcttcaat tcttcactta
    16321 cctttaaacc ttcgcagccc gctacgcagc acttgcccat tcctcttgcc acaacagctg
    16381 catgagaggt cattcctcct cttgcagtca gtattccctt agccattgcc attccttcaa
    16441 tatcttcagg agatgtttca agccttacaa gtattacttt ttcgccgttt tttgatgctt
    16501 caactgcatc ggccgctgtg aagtatgctt tccccgaagc agctcccggt gaagccggaa
    16561 gtccttttgc tattgataat gcctttttga gtgcagctga gtcaaagttt ggatgcaaca
    16621 gttggtcaag ctgtgccggc tctactctca ataccgcttc ttccttggtt attttgcctt
    16681 cctcaacaag ctcaacggcg actctgagag ctgcagcagt tgtcctcttc ccgtttcgtg
    16741 tctgaagcat gaaaagcttg cccttttcta tagtgaattc tatatcctgc atatctctgt
    16801 aatgattctc caggagattt gccacactta tgaactggtc ataaacctcc tgatttattt
    16861 cgctgagctt ttcaatggtc tgaggtgtcc ttatccctgc gacaacgtct tcaccctgtg
    16921 cattcatcaa aaattctccg aatatcttct tttctcccgt tgcaggattt cttgtgaaag
    16981 caacacctgt tccgcagtca ttccccatat ttccaaatac cattgactgt acattaacag
    17041 ccgtacccag gttcccgggt atgttgttca tggccctgta tattattgct ctttcattat
    17101 tccaggacct aaaaactgct gttacagcca gcagcagctg atccttcgga ttctgaggaa
    17161 atccctcgcc cttctccgcc ttgtagatac tcttgaactt ctctactact tccttcaagt
    17221 cggaagctgt aaggtcggta tcgtattttg cacccttttc ttcctttatt tcatcaagta
    17281 ctctttcgaa ttttgacttg ggaatctcca ttacaacatc gctgaacatc tgtatgaacc
    17341 ttctgtagct atccattgca aatctttcat tgtcagtgat gtcttttaca gtcttgacaa
    17401 cctcatcatt caaaccaaga ttgagaattg tatccatcat gccgggcatt gaaaacttcg
    17461 caccggatct taccgataca agcagaggat tgcttcctcc gccgaaagtc tttcccgttg
    17521 tggcttcaag atctgtaagc ttttccatta tctcatcaat aagttcgctc caaagctgct
    17581 cccctttgtt gtaatactca ttgcaagcct ctgtagttat agtaaacccc ggaggtaccg
    17641 gcagccctat attgctcatt tccgccaaat tggcaccctt gcctcccaag aggtctttca
    17701 ttgaagcttt tccttcttta aaattataaa catacttctt cattcctgtt ctgcctcctt
    17761 taatatttga agtattgtat ttgctgtctc ctcaattgcc ttatcggtta cgtcaactat
    17821 tctgcaacca attttgttca tgatttcctc agcatattta tgctcatagg ctattctttc
    17881 cttgctggca taattcgcct ggtcgcttag cccatgagat ttcaaccttt ccagtcgtat
    17941 aggattaagt tggtctgtac ttattaccaa accaattatt ttcttcggcg atatttcaaa
    18001 aagctccttg ggcggcttta cttccggcat cagcggtata tttgccactt ttaaattctt
    18061 ggttgccaaa aacataccca gaggggtttt ggaagtcctg gaaataccca gcagcaccag
    18121 gtctgccttc ttgatgcctc tcggatcctt gcagtcatca tattttaccg caaattccac
    18181 ggcttctatc ttgctgaagt attcatcatc aagctttctt gccgcgcccg ggatatcagt
    18241 cggatgctgt cctgtaactt tggcgattgc ctctacagcc ggccccatta tgtcaacaca
    18301 gataatatta tgcatagccg cctgttcagt aagatacttc ctgagctcag gcacaacaag
    18361 agtgaacatt attatactgt ccaggtcttt ggcctcactg ataacttcct gtatgctctc
    18421 tatctcggta acatacggta tccatctgat cctgatttca cattcgggat actgggcgga
    18481 tgcggctttt gccacttgtt ccgcagtctc acctattgaa tctgatatca caaatattac
    18541 tgccctgttt ttattcataa tatcccccta acctattgct tatataaatc atatagcagt
    18601 ttagtcatat tggttttgga caccttgcct gttattttga actgttcttt gccatcactg
    18661 tctttaaacg gttccactac cggtatactg tctatttcat gggtgataag cttaactact
    18721 gcatcatatg cgctgtcatt atcgaatata tagactatat taggcatcct tgacataatc
    18781 attccaatcg gcatattttg tatatcatta ttaccgattg caatctttat gaaatccttt
    18841 cttgatacta ctccggaaag tgctccttta ctctgcacgt atattgtacc tgcatctctt
    18901 aaaaaaagca tgacaataga atcatatact gttgctgatt catccatgac cacagcactt
    18961 gacttgattt ctgcaacctt aatgcttttt atgaaatcat ggccaatgtc gttaattatc
    19021 ttgccggagt aaaaatagcc aaccttcggc ctggcctcaa gcatatcgga cattgtcagt
    19081 attgtaagat caggcctcaa tgccgccctt gtcatattaa gctttgccgc cagctgctcg
    19141 cttgtgattg gttggtattt ctttaccaat tcaattatct gcttctgtct gcttgtaaat
    19201 tctattgcaa ccacctgcct caaggaaata ttcagagcca tcctgtctgg ggctgtgtat
    19261 gcactcctgt ttattcagcc caatttttta ctttattctc attaaatact atcataatgc
    19321 attgacaagc acaacaaaaa tcaattattt attcatcact acagcactca aatcagcaat
    19381 tctgttcatc atgtccccta tgctctttaa aagccccagc ctgttgttcc tgattttctc
    19441 atcttcaacc attaccataa tattgtcaaa gaagtcgtca atgggttttt taagtgttat
    19501 cataagctcc agagcctttc cataatctct gttgtctacg cagttcacaa attttgcata
    19561 aactttatcg tagtcatgca aaagtttcag ttcagccttc tcagtaaaca gcaactcgtt
    19621 gatttccgtg ctcttgccct tagatgccag gttggatacc cgattgaagg aacccagtat
    19681 gttcatgaac tcatccttgt acttccattt tgaaagctcc agaatcttca ggtatgagtc
    19741 atatatatcg tcaaacccgg catttataac agcatcaata acgtcatact caaagccctt
    19801 gtctatcatg acatttttga gtctttgcct gaagaattcc aatatgtccc tctttactga
    19861 cttttcatca cccttcaata tccctttttc ttcaaaaggc ttaagggaat tatgaattaa
    19921 ttgcgccaaa ccaagatgaa tgttactgtc gagtattatt gacgtaaccc ctattgcctg
    19981 tcttcttaag gcatatggat cctgagagcc tgtcggttga attcctatgg caaagcaccc
    20041 gcatatggtg tcaattttgt ctgaaatgcc aagaattgaa ccattcaatg tttcgggagt
    20101 acaatcccct gcaaacctcg gcaggtaatg ctcaaatatg gcatccgcca cttccctgtc
    20161 ctctccttgc agcagtgcat actcccttcc cattatcccc tgaagctcgt caaattcctt
    20221 aaccatattg gtgactaaat cagctttgca taagtgtgca gacctcagca gattcttttt
    20281 cgcaccggcg tttaatccca aaatcgcagc cagatattct gctatggcag aaattctctg
    20341 agtcttatca tatattgttc ctaaggtttc ctggaatacc acattcttaa gcttttctgc
    20401 gcaattatcc aaggattcct tcctgtcctc gctatagaag aaatccgcat cagccagtct
    20461 tgccttaaga accttctcat tgccttcccg caccgtttcg aggaagcggc tgtcaccatt
    20521 tcttacagtt atgaagtaat tcataagatt cttatcatta tccacaaccg gaaaatacct
    20581 ctgatgctcc ttcatgggag taattactgc ttccttggga agcttaaggt attcggcatc
    20641 aaaaccgcca ataagtgcgg taggatactc tacaagatat acaacctcct ccagcagctc
    20701 ttcatctata agcacttccc cgcctttttc cctggccagc tcctcgcact gcctgcggat
    20761 tattgcgcgt ctttcctcct ggtccgctat tacaaagcct gctttcagct tatcaaaata
    20821 ctctgcagtg ttatttatct taatactgcc tttagataat accctgtgcc ctttagtata
    20881 ccggctgcac tttattccgt ccttgtcaaa ctttatcagc tcatccccca gtattggcac
    20941 cagccatctg acaggccttg cgaatctgaa ggacttattg ccccatttca ttgacttggg
    21001 aaaagatatt gaagtaagta tatcggggag tatggttttc agtacttttt taacaggctg
    21061 ccccttttcc tgcttcttgt agtatacata ttccacaccg gaaagctcct gtataaaaac
    21121 atcaccaagc tctgccttcc ggccctttaa aaagcccagc agcgctttgc tcggatttcc
    21181 gttttcatcg taggctgccc tcctggaagg ccccttcacc agttcctcca gattctcctg
    21241 ctgttccgca atgcctttga caaacagcac tattctcctg ggagtaccat aggcctttat
    21301 ctcttcaaaa gctatcctgt aatttttaag cattgctccg gaaatacttt tgacctgctt
    21361 cagggttgaa ggcatataat ttgcgggaat ttcctcagtg cctatttcga ataacaaatc
    21421 cttactcatt tttttcacct ccgaatacag ccttcttgag aagaggatat cccaattcct
    21481 ctctctgctt cagataacct tgagctacaa gccttgccag atttcttacc cttccgataa
    21541 agccggtcct ttcagtaacg cttatagcct ttcttgcatc aagaatgtta aaagcatgtg
    21601 aacatttcag aacgtagtca tatgccggaa gtataaggcc tatatccagc agccgttttg
    21661 cctctgcctc atattcgtca aaaagtgtaa aaagcagttt ggtatcagct tcctcgaagc
    21721 tatactttga atgctcgaat tccgctgtct tgaatacatc tccatagttt atccccgaaa
    21781 cccattcaat atcaaataca ttacttaaat cctgaatata catcgcaatt cgctccaaac
    21841 cgtaggtcag ttccgcagac tccagctgac agtctatgct gcctacttgc tggaagtacg
    21901 tgtactgggt tatctccatt ccatccagcc agacctccca tccgagtccc caggctccaa
    21961 gtgtgggagc ctcccaattg tcctcaacaa accgtatatc atgcttgacg gggtcaatcc
    22021 ccaatgcttt caggcttccc aggtacaaat cctgcacgtt gtcgggagag ggcttcaata
    22081 ttacctgaaa ttgatgatgc tgatacagtc tgttggggtt ctctccatac cttgcatccg
    22141 caggcctcct tgaaggctct acgtaagcaa tcctccaagg ctccggcccc aaagccttga
    22201 ggaaggtata tggactcatt gtgccggctc ctttctccac atcataaggc tgtgcaataa
    22261 tgcagtgccg gtcagcccag tatttttgaa gcgtcaggat tatttcttga aatgtcattt
    22321 ttcatccctc cataataaaa tagaaaaacc tttcatccct tgaagtaagg gacgaaaggt
    22381 ctgcttccgc ggttccaccc tttttgactt gaaatcaatc aagccctctt ttttaagctt
    22441 atgctccaaa gtgcccgtaa tcacatgcac tgccggctca caccatgtgc ttcatccggt
    22501 gaagcacccc cggttctctg ataatgcact tcctgattac tcctcttctt caacacattt
    22561 tagcattaac actaatttag caaatcataa atattttgtc aataatcaat atgttttaca
    22621 ctaaaccttt tacattgaat tcaactagtg taaaacattt tccttgattc atatgttgtg
    22681 aaaaatacat tactttaaca caaaatcaga atctatctga ttttgtaatg ataaatcttt
    22741 ttcacaacat atattattgc ttaaatgtat tgtcggtatt gtcattgaca gctccgaaaa
    22801 tatccgatgc atccgccgcc gcagaattat gtcctgcttc cgatgatgtc atgtttctga
    22861 aatcatctgc ggcatttttg attttgcctg ccaaattatc tgctgtattt gtgattgctt
    22921 cagatacatt aatcgtctgt ttgtttggcc tttctacttc tattacgcat tttgaaagca
    22981 gcgcaactgc agttcctatt gccgtaagct gcggtatcac cactgcgctt attgctcccg
    23041 cagttacagg aatgtcaagt attacatagt catctttctt tactcttatt ctgttcacgt
    23101 tgccggattt gactattccc ttcagctttt caacagcctc gctgcctttt accgagatag
    23161 tctccgtcca ccttttatct cccgcctctt caatatttat caaggcatcc actacattgc
    23221 cgtttgcggc ttccagagct tccttggctt ccttataggt cactcctgtt ctatccctga
    23281 tgatatcaac cttttccaga cttatatcca tggaacttac tcctttcaga acattaataa
    23341 atttacttct gggcattcaa atccttcata gagtctaaaa aatcaaggga cttaaacctt
    23401 ttatccaggt gactttggat gaaggatctt gttatttttt caatttcatt aaaaatagta
    23461 ttatcaattt tcttagtatt tagtctagaa atttcaatgt tcaacaaaaa attaatggct
    23521 tcagccgtac cttggcttat cctgaaaact tctccggcat ttttttgatt ttcaccggtg
    23581 ctgcagctgc cgcatattac tccgcccaaa tcaacactga atcttgtata atcctcactg
    23641 ctctttctgc atatggcaca cctggcaata ttaggcctgt acccgcttat atccataagc
    23701 ttgagttggt agccgacacc cagaatccct attggtatat tttcggttga aagatagtat
    23761 agtgtttttg caagcagcag gaacagtctt tcactgggaa taccctcatc ggaaacagct
    23821 tctgcaagct cggcaaaata tgtggcataa gaaagcctaa gcaaatcctt ccttatgtta
    23881 taaaaggttt ctctcgttac tgcctgggaa atgtgataaa agttcgtgcc cttatataat
    23941 acgaactcac tgaaaacaaa aacctcagac gatgaaagaa ggctgctttt aggttttctg
    24001 cagcctttgg caattgcttt gatttttccg ttgtttctcg taagtatcgt aagtatctta
    24061 tcggcttctc ccaggttggt atacctcagg actatacctt ccgtcttgga tatcggcatt
    24121 aaatcactcc caattatatc cgaagttctt aagtgcactt ctgctgtcac gccaatttct
    24181 tctcaccttg acccaaagct gaagattgat cctgcaatgc agcaggttct ctatgtcaac
    24241 tctggatctg acacctatct ctttcagcat cctgccttgc ttgcctatta ttattgcttt
    24301 gtgtgattcc ttctcacaat atattgtagc agcaatatcc atgagatcct tattttctct
    24361 ttctttcatg gaggtaattt ccacggcagt gccatgaggc acttcctctg atgtaagctc
    24421 aagaaccttc tctctgataa tttctgatac aatgaacttc tcaggcatat cggtatatat
    24481 atcctccgga aaatacctgg gcccttccgg cagatgctta actatttcct tcagaagctc
    24541 atccagattc tccccggtgc ttgccgaaac aggtattata ctgtcaaaat aatcttcatg
    24601 cattattaag gaagctgcaa tctgttccag cctggatttg ggtatcttgt caattttgtt
    24661 cagcacaagt ataaccgggg agttaacttt ttccgccacc tgtgcggcat acatgtctcc
    24721 cggccctact tcagctgaac agtcaaacat gactacaact acatccacgc ctttcaagga
    24781 ctcggttgcc gttttcacca tatactcccc caacatgtgc cttggcttat gtataccggg
    24841 agtatcgata aaaactatct ggtactcact tgtggtatat atgcagcgga ttgagttcct
    24901 tgtagtctgg ggtttgctgg aaataatgga tacccgctcc cccagcagct tgtttataag
    24961 agttgatttg cccacattcg gccttccgat gattgtcaca aatcctgatt taaattttgt
    25021 gttcataata ctactcctta tatgaagaat gcgacgcatt cttcgggtac gaggtacgag
    25081 gcgcgaggtt cgaggtttag ggcagggctt cggagttatg ataacactga agacacgaaa
    25141 aaccgctaaa tgcacggcgg gataaatatt acgttacata aataattatc gataatgatg
    25201 taagtatcga aattttctta tgtaactata taatatataa aagttaccat gcaatcgcgc
    25261 agcgattcca tgaaacagcc gtgtctcccg tgttacccat tacctacgtc gcattatcct
    25321 actaatgcgc ttagaattct gtaaaagcct cgggtaacaa ctccccgatg gtatgaaccc
    25381 ttacttcatc tccgctcttt gtaattagct ttatgtccaa accccattcg gacatgaact
    25441 gcctgcaaat tccgcaggga tacgtgaagc ccttcaggct gctggcaacc gcaatggctt
    25501 caaactccct ctctccttcg gatatagcct ttgtaaatgc aactctttcc gcacatatgg
    25561 ttgcaccgaa ggatgagttt tctacattgc agccggtata taccttgccg ctcctggtca
    25621 gcagcgccgc cccgaccttg aaattcgaat atttcacata agcattttcc atggcttcct
    25681 gtgctttttc aattaacaca tcgtaattca tgcctatcac tccctgattt caaccttcac
    25741 atagtccgga accacctgta cccatgtaac tcccttgttc ataagcactt cattgccctt
    25801 aaggtccttg tatacctcct ttgcgctcct gccgtccttg ttccattcaa tttctatcag
    25861 cttccctccg ctgaggtagt atcctctgcc cttgccgata ttctttattt ccaaccgtcc
    25921 tacatcgtca agaacctttc tggtggtttt ttgaacaatt atattggctg cagctatgtc
    25981 ctttcccgat tccttgtctt tgagccttgt tccgtttatc gtcctataat ataatttgtc
    26041 cttttcacta tattcatacc cgacaacata attcttgctg taattaaata tgattgatcg
    26101 ggtagccttt ccatccggtt caaggaattc ttcgttgaac ttgaaatact caggtgccgt
    26161 tttgttattg aatttattgg tttccattac ttctctcata agcttggtgc ttgtatacag
    26221 gttatggggc atcttcctgt cctttgacct ccagaagcat ggggaccctt tcaagtcatt
    26281 gagggtattt acttttagcg tgaccaggtc ctgaagggcc tgggggctcc cgccgcagtg
    26341 cacgtatact gcattgaatt ccagggcttt atctatgaaa taaggcctcg cacttctcac
    26401 cgggccgact ttgtctgcat gagtgtggtg aaaaattgcc atatatctcg tgatattgcc
    26461 ttcaacaggg atttcataca ctatctccgc ctctgaaagc cctgactggg gtcttgcgtt
    26521 atattcattg tcaatcatca cagctatagg cctgagcttc aaatcagcct catatgttac
    26581 caactccccc gtcaatgggc aaggcgaccc cttgggcggt tcgggagccg gaggcggact
    26641 cggctcgaca accggttcag gtgtgacctc cggttcagta cttgatacag gttcagtggc
    26701 tttaccgcat ccggaaaata ttactgcgat aatacataaa atcatcagca taaatttaca
    26761 tctgttattt ttcatatgag accctctttc attaaataaa atcacaatat tcctaagcaa
    26821 tctccatatt cttcagtatt tcttcctgct tatcgaatat ttctccctcc gaagcttctt
    26881 catcatggtc atagcccaga aggtgcagca tcccatgtac cagcaggaaa gccagttccc
    26941 gcttaaagct gtgcccgtac tcctccgact gctccgctgc tttttccatt gatatgacta
    27001 tatcccccaa tgcaatttcc tcttctatat attcggcacc ttcatcctct gtattaagct
    27061 cttcttcgat gaactccgct aacggaaagg ataatacatc cgtttccttg tcaatattcc
    27121 tgtattcttt gttaagcttc ctcatctctt gattgttcac gaaagagata ctaacctcga
    27181 atgcctcttc gcactgctca taggcaagaa ccttttcaac tacttgtacg acaaatgctt
    27241 ccaaagcctc atcaactttt atttcttcct gtctgttgtc tatcaatacc gacatataat
    27301 tacctccaaa tattatttac ttcgtacttc aaagcccgcc ctaattcccg tatctcgtat
    27361 ctcgcaccct gtaccccgga cctcgcgacc ccgtaacaca ttattgtaca tctttaatag
    27421 tttccggata ttctattcta tcatggaata ttcctgccag caccttcacg aaagcctgtc
    27481 ctatcatctc cagatccttt agcgtaaggt cacagttgtc cagttgtgca tcattgagct
    27541 tatccttaat tatttttctc actccttgtt caacctgatc ccgggtaggc tcggataatg
    27601 acctgatata ggcctcaacc gaatctgcca gcataactat cgcagcttct tccgtctgag
    27661 gctttctgaa tggatatctg aacttactct catccactgc ttcggcccct tctgcactta
    27721 atgccttgtg atagaaatat gctaccagag tgtttccatg atgctcctcg ataatcctta
    27781 ttactgtgga ggggagcttg ttctttccgg caatctccac tccgtccttt acatgggaag
    27841 taatgaccag ggcacttagg ttgggagtaa ttttatcatg ggggttatcc ctggtaatct
    27901 ggttttcctt gaagaaataa ggccttttga gttttccgat gtcatgatag tatgccccta
    27961 ccctgcataa aagcgcattg gcacctatgt cttccgccgc cgcctccgcc aggtttccca
    28021 ccaatatcga gtggtagtag gtgccgggtg cttcgataag cagcctcctc agcatcggct
    28081 gattcgggtt tgacaattcc agaagcttta aaggggtgat gatatcaaat accgtttcaa
    28141 aaaacggcag cgtaccaatg gtaaggactg ctgaaagagt acctcctatg acaccgtaca
    28201 acccgttgtt aatcatcctg tatatatctt ccctgttcag aatgccttca ctgaatatga
    28261 ccagcaggct gaccaggctt acggccaatc ctgacaggat aaaggtactc ctgtggtgtg
    28321 tttttgatac cattcccgcg ccaagtatac cggtcaggag catcatgacc actacatgca
    28381 agtcaaagcc tccaagaaag cctgcaagta tggacgtcat tatattcgtc attatggcta
    28441 ttcttgggct taccaatatc gagacaagca ttgcccctgc gggcagtact atgagatact
    28501 gggaaatatt tttgagcatc atggtaacag tcaatgccat aatggctgtc aagctgataa
    28561 gaagtaaagt agaattgctg tcccaaagct ttttgtcaag gcttcgcagg tacaggccca
    28621 taataataag acaaatgccg attattaccc catagcctaa atagaattga atgtcaaatc
    28681 tgtccttatc actttcggcc agaagcccat aggcattgag cagctgtatc tgctgagatg
    28741 ataattcctg ccctttgccg actatcagag tacccttgcg gatgactaca tcttccacct
    28801 tttcccgctc gctctgtttt cttgccaatg ttgtctgctc atcatagtac atatttggtt
    28861 tcagaaccat tacggcaata tccgcgccaa tttcattaag ctggcttttc ttgggaagat
    28921 tcataaagaa ttcccttata tcgcttctct tgtcatttag ctgctgttcg gtaatcctcc
    28981 cgctaagcat ctggttgttt atatatttga tattgtgttc caaagatgcg agatctgctt
    29041 ccgaaacatc aagaagcata ttatacaagc tgtttccaag gcttatgttg gaattttgtt
    29101 taataaggtt aagcttctgc accttatcca gctccgcatt actcctgact gaattcaaaa
    29161 gcataaaaaa gtcgcttatc ttcttttcta tatcgatctg aacagtcaaa tccaggttgt
    29221 aaaccggttg gacactatcc tccgcttgtt ttctccttag ctcggtggcg gacttgtcca
    29281 caatatctgt tggagcatat atgtctttat cggatatatc acctacctgg agaagatgtt
    29341 tctgcggcgc agtattcaga acaaacagta aaaacagcgc aataaaaagg cctgcaacca
    29401 gtaatgctct gattgtagtg ctgtttcttg ccagcctttt taaatccgaa accgaatact
    29461 ttatcattcg ctctctccta tttcctcttc atgcttttca taagccttta tgatatcctt
    29521 taccagctta tgcctgacaa catccttttc actgaagtat acaaatccta tacccttaat
    29581 acctttcaat atctccatga cttgcttcag tccggaatat ttccctttgg gcaggtcaat
    29641 ctgtgtaatg tccccggtaa taatggcctt ggaaccaaag cctaatctgg tcaggaacat
    29701 tttcatctgt tcaggggaag tattttgcgc ttcatccagt atgatatatg agtcatcaag
    29761 agtccgtcct ctcatatatg caaggggtgc tacctctatt acccctctct ctatatgctt
    29821 ttgataggtc tccgcaccca ttatgtcatg caaagcatca tataaaggcc ttagatacgg
    29881 atctaccttg ttctgcaggt ctccgggcaa aaaacccagc ttttctccgg cttccactgc
    29941 aggccttgta aggattatcc tgcttacctc atggcttttg aatgccttta ctgccatagc
    30001 cattgcaaga taagtcttcc ctgtccccgc agggcctatt ccgaatacaa cctcattgtt
    30061 gcgtattgca tctatatacc tcttctgtcc tattgtcttg ctctttattg ctttccccct
    30121 ggcgtttgtg attataacac tgtcaaactc ctcactcctg ttttccctgc cctcttttac
    30181 aagttcaata gtatagctta ccgcatggct tgtaacagct tcaccgttct cctgtatttt
    30241 cataagagta ttcagagttc tttccgccat cgcaacggcg ttttcttgtc ccacaatctt
    30301 tacttcgccc tctctgacaa ttatgtcaat attgaactca tcttctatga cttttagatt
    30361 ctggtcaaag ttcccaaaga gattcattat tctttccata ttatcaattt gtatactagc
    30421 ttcagtaaat tgctcctgca attgttctcc tccttatgtg cttcggacac catgcatgtg
    30481 gtgcggacac catgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggaca
    30601 ccatgcatgt ggtgcggaca ccatgtatgg tgtccctact agtaaatttt ttgtttcacg
    30661 ccaatatcct caagcacctc gacacttatt tttgccttga cagacttttt atctgtgtaa
    30721 tagtttatat ctcttttcag aattacggca tcttccggta tccgaagctt tattcttgca
    30781 ttcagccttt ccgcgcatct ctccttggct tcgtcaacca taattgtctt tagttcgggt
    30841 actaattctt caaatctgta ttcaataagc tttattggaa atacataatc tccgaagctt
    30901 atcaggtgcc tctcttccac atattcgtta aaatccttga agttcacgct tttcttcctt
    30961 gagaattctt tgtccagcag cttcagttta tactgccgtg caacccttcc ggtcggcttg
    31021 aattctattt gctgaagggg tatttcctct gcgtcctcat accatgtcct cgcacttact
    31081 tgagcaagag aatgtatttg cctgggttcc aggttttctc tcaatattgt gcctgtgacc
    31141 agcttctgcc cctttctcac agaatccccg tccttaacca ggggatcgcc ggaaagcacc
    31201 agcagctttg ttatcactcc gtcttttgcc gcaacaatgt cgcaggcatc agtgcggctg
    31261 atcagtggag gcccttcact tttctcaaca acctctacca ctgccttggt accttttatt
    31321 tgtatcccga tccatgaaag ctccggcatt tttatcagca cctgattctc aatatgcctt
    31381 ctgtccagcc ttcctttcca gcttccttcc ttaagccctt cgttataaag gcatcttaat
    31441 atgctctcat tatcgacatc cttggttcca actatttcaa tcatccagac aaaggaggac
    31501 attatgtaaa taaatacaca catcaggaac aggccggaat agaatagctt tcttcttctt
    31561 atattcctta ggataaaagg aaagccccgc ttgttcaata tgctgactga actgtccgtt
    31621 tttcgtacaa tgtccctgag ccttccgaaa ttctccagcc ctatttttgc cttgaccgtt
    31681 gtatagtcaa gcttctcgat atcccatatg taaatattat tgactactgc aagattaata
    31741 aacctttcaa tcttcagtcc tttaacaata ataatagcat atcccctcaa ataattccat
    31801 attcgtatga taaacatgcc tcttataccc ccagcaactt caatgatact tagaattcca
    31861 tattcgttat ttctcccgtt atcgtaattt cctcctgaaa tattgttttt attgcaaggt
    31921 ttttaccgtg aagggcgact ataccgctgc cggtgttgat tctaataagc tcttcgctgt
    31981 attcgattat acccttatgg ttttccaccg ttatctgccc ggtaccaatg atgattattt
    32041 tggaaacatc caggacaata tccttgggaa gttcgaaagt attggacagc ttctctctta
    32101 atttgtaact ccttttaccc atagcacaca cctctgtata aatgtatgcg aaagaggtac
    32161 tttgaagaac aaaaatagga ttgccgcaca ataggacatg gggacgtttc tcctgtcccg
    32221 ctttcagcgg gacaggagaa acgtccccat gtcctactcc tttttgacgc ttatcttatt
    32281 cttcttttca ttgctctggg gggccccaga acctcagcca tgactacgct tctaagaagg
    32341 ttatcctctg ctatttcaaa tatgaagcct tcttcctccg tttctcttat ctcagccttt
    32401 aaggcatcca acctgctgtg ttcagggtgt tcatcgcaga taccctcgga gctgctgcta
    32461 tgctctacat aatccaagct gccgaccctt cttctgtctt caatctccat gccttcatag
    32521 tcttcagcag gctttgattc cggcacatgt tcctccggac ttccgaataa ctcattccag
    32581 ctttttccca gatctgcgaa aggcccttca gaccactggg gaatatgctt ttgtctttga
    32641 accggcttct gccggtattg gccttgctgc cggttcatgc ctcttctgcc cctgccctgg
    32701 ttcttctgct gctttgcagc aaggttaaac aaggctatta ttatagctat tactatatat
    32761 ataccttcca actacatccc tcctttgatg gagatgctat aggggctatt tctt
//
LOCUS       DAOOAB010000029        31462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1868_length_31462_cov_13.906072, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000029 DAOOAB010000000
VERSION     DAOOAB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 31462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31462
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_31462_cov_13.906072"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..391)
                     /locus_tag="PKU88_07605"
     CDS             complement(26..391)
                     /locus_tag="PKU88_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69178.1"
                     /translation="MSDIDKVYDLLEKVYVELQDTKNEVKNNSQRLDNLESGQNKLEH
                     RMGTIDNRMSKIETTLEHDIKAKLQALHERTGDNTVKLGEHTERLESIENKIDILAMS
                     INSQDKRLEVVESRHKKAK"
     gene            complement(569..1060)
                     /locus_tag="PKU88_07610"
     CDS             complement(569..1060)
                     /locus_tag="PKU88_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69179.1"
                     /translation="MANIKINLNGLEIMVFFWNSLMSREKVSESFLNDLCSMQEFSYI
                     YNEDFNNESLRRALSALNNKEPFSGNKPERKYYSNSLMIMEYIDEVQPAINAIKKLNL
                     NSIAEKAKEDVEVVVVPGLSKGIEKTDNKLIVDFFGLKNTENGLVISGESLEAVLKNM
                     FEV"
     gene            1332..2123
                     /gene="arsM"
                     /locus_tag="PKU88_07615"
     CDS             1332..2123
                     /gene="arsM"
                     /locus_tag="PKU88_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1944..1946,aa:Sec)
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPX69180.1"
                     /translation="MAKIKTDIRNAVRRSYGRIASGRAKDGGCCGSNISVKESAKDIS
                     RGIGYSEEEMQEIPEGADMGLGCGNPQLIAEIKEGETVIDLGSGGGIDCFLASRKVGP
                     KGYVIGVDMTPEMLSKSRILARKHRYFNVDFRLGEIENLPVADNTADVIISNCVINLS
                     PKKQRVYDEVYRVLKKGGRIAISDVVLVKELSNEMKQDEKLYCGUVAGASSVEELKAY
                     LEKAGFSNINIDTKEVSEEYAAKWSHNLKVGEYIMSASIKAVKAK"
     gene            2149..3330
                     /locus_tag="PKU88_07620"
     CDS             2149..3330
                     /locus_tag="PKU88_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPX69181.1"
                     /translation="MPELSSKVNIFTDSVIRRMTRISEAYGAINLSQGFPDFDPPKEI
                     LDALAYAAYNGPHQYTFTYGSYNLRSALAKKHGKAINRTIDPDMEVLVTCGGTEAMMS
                     AMMTICNPGDNVIIFSPYYENYRADTILSGANPIIVPLNPPDYSFDKQVLEDAFRNNV
                     KAIIVCNPSNPTGKVFTTEELEFIGHLALRYDAYVITDEVYEHIIYEPFHHVHMAGLP
                     DMFEHTITCSSLSKTYSITGWRLGYLIGPEKVIESAKKVHDFLTICAPAPLQEAACVG
                     VNFGESYYKDLQRKYTEKRNYFCRRLDEIGLRHNTPQGTYFVMMDISEFLSMDKFKGW
                     TDLQFCEWMIMEIGVAAVPGSSFFMEPVNNLIRLHFARSMDTLDEVLNRLEKLGRYLH
                     R"
     gene            3366..4211
                     /locus_tag="PKU88_07625"
     CDS             3366..4211
                     /locus_tag="PKU88_07625"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPX69182.1"
                     /translation="MRELNVDVITNTVENLCIESNYFLPRDVKKALEDAVLKEESPVG
                     RETLLDILKNAEIAARNQVPICQDTGLTVVFAELGQDLRLVGGDFYEAINEGVRRGYK
                     NGYLRKSAVDDPFLVRKNTGDNTPAIIHTTIVKGDRLKIIIAPKGGGSENMSALRMLT
                     PAEGVDGIKKFVIDTVDKAGSNPCPPVIVGVGIGGTVEMTALIAKKALLRTVGEHNPN
                     TEVAKLENELLEEINNLGIGPQGFGGRITALAVNIETFPAHLASMPVAVNIQCHAARH
                     KEAVL"
     gene            4234..4782
                     /locus_tag="PKU88_07630"
     CDS             4234..4782
                     /locus_tag="PKU88_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPX69183.1"
                     /translation="MSRIINTPLTDEVTCNLHAGDRVLINGIIYTGRDAAHKRLTELL
                     DRGEKLPFELNGQVIYYVGPCPAKPGNVIGSAGPTTSCRMDVYAPGLMEMGLKGMIGK
                     GSRSKDVIDSIVRNKCVYFAAVGGAGALLAEAIKEAEVVAFADLGPEAIFKLKVENFP
                     AVVIADTEGKDLYKIGRSKYQR"
     gene            4795..6027
                     /locus_tag="PKU88_07635"
     CDS             4795..6027
                     /locus_tag="PKU88_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPX69184.1"
                     /translation="MMSLREDALKLHRDNRGKLQVVSKVSFNDKENLSLAYTPGVAEP
                     CKEIQKDPDKVYEYTFKGNMVAVVTDGTAVLGLGDIGSHAALPVMEGKAVLFKNFGGV
                     DAFPICLNTIDADKIVESVTLIEPAFGGINLEDIAAPACFEIENKLKKKLSIPVFHDD
                     QHGTAIIVLAGLLNSIKITGKKLQELAVVINGSGAAGIAITKILLSAGVKDAILCDRA
                     GIIYKGRNTGMNWAKEEIALVTNKECRKGTLADAMVNSDVFVGVSSANIVSEDMVRSM
                     NKDAVIFAMANPVPEIDPEPAKKAGARIIGTGRSDYPNQVNNVLAFPGVFRGALDVRA
                     SEINEEMKLAAAYALSEIIPADELTEDYIIPKVFDPRVAPAVAKAVAKAAIETGVARA
                     AVDPEQVALRAAGLIKLK"
     gene            6052..6477
                     /locus_tag="PKU88_07640"
     CDS             6052..6477
                     /locus_tag="PKU88_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPX69185.1"
                     /translation="MVIKQTIIRGINNALKVILDLIKYIIPAVFVLKVLEHSGWLVRI
                     ADLFGPFMSYIGLPGEGALIIVMGQVTLYSAIAAMAVVDITVKQLTIVSTFVTIFHSF
                     ILETAVINKAGGNGFMIIGIRVIAAVLVCFILNILIPGV"
     gene            6502..6960
                     /locus_tag="PKU88_07645"
     CDS             6502..6960
                     /locus_tag="PKU88_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69186.1"
                     /translation="METFEISFSTIFKMTVIVVPLLIGIEYLKEIGWMEKLSVKLRKV
                     SELIGLPGEAAFGMIVVYFIGLVFGSGVIIRMKKEVGMTRKQLNTMFIFIGICHAVVE
                     ETIVFTAVGANGVIVMVSRVLASFLFGLLYAWFAKHDETSIRKSSGIELE"
     gene            7009..7668
                     /locus_tag="PKU88_07650"
     CDS             7009..7668
                     /locus_tag="PKU88_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69187.1"
                     /translation="MKKGSIIWIIALLLWILVLAVPATRSTFMAATDAHPYVMGFIKF
                     AILATMGDLLGIRVLGGEWIIPRGVFYKSIIWGIIGMMTTLVFTVYMGGTSAAQAAGR
                     LPFKESALALAFFTSAIMNLTFGPIMMAFHRFTDMYIDSRYESSKGRTTISSLIDRND
                     WHSLVEFSWLKTCPFFWIPAHTIVFMLPAQYRVLISAFLSIALGLLLALAKKGKAAKA
                     A"
     gene            7682..8188
                     /locus_tag="PKU88_07655"
     CDS             7682..8188
                     /locus_tag="PKU88_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69188.1"
                     /translation="MRRVLAIVAASILILLAAGCIKEADTYLYKGRDGDWSIDLQKEF
                     TKSKEEYDEELKSYTITFKTESDLFFVINEIIDEKLEINQDRIKEELSLDDYMKAERY
                     DNIDIPDIGTAYGALVFDEATGTAMMYHRLRYKDKVISFIFHYKNDLSDEHEAKAKAI
                     IGSFKGLK"
     gene            8318..9004
                     /locus_tag="PKU88_07660"
     CDS             8318..9004
                     /locus_tag="PKU88_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX69189.1"
                     /translation="MANEGENVNRKRLIEAFLKIFLWVVAVIINIMYISGLFEHLPEE
                     LKPFIITGDVSMVVILLWFMPSTMAERRLKKGLEYLDRDNDKAVRHIEAYLDSKMLTM
                     NEIKHALRVLGVAHHKRGDDAAAIKCLTKALEGNYKDNDLKAEILGAMGIIYSESGEF
                     QKAAECFDRTFEIMFAMSKANIDKAIFIQVINTYIKTGQKEKALMIYDRLLMIQGFTR
                     DKRVEEILGI"
     gene            9136..9303
                     /locus_tag="PKU88_07665"
     CDS             9136..9303
                     /locus_tag="PKU88_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69190.1"
                     /translation="MNKRWNVFVTRMLPHIGSATIEARINMGMIAARNIIAAMNGKVP
                     KTLVNTDVLKK"
     gene            complement(9293..12262)
                     /locus_tag="PKU88_07670"
     CDS             complement(9293..12262)
                     /locus_tag="PKU88_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPX69191.1"
                     /translation="MKHLVSSLICIFLVLMSLFQINIYAENNDISITPSIIFEDSSES
                     YPIDGEQVILYTNKYGEFTRPFGAHTHEFVIVNNIVVQENSNGANGTYIPPNGCVISY
                     TGDNEDFRRKFSVGTGLTLTNIDIPVSPDMYFILEDMLIPIDQINTVRDANQIILYNA
                     AYNTSTKTNPWGMELTVEDGIITKIADMTNTEGLQGRNNSQIPTDGAVISIHAGSPYY
                     MQVREKAKPGININISADAKLYNASKIKYAAYNPRTLADNPAAWDSMKEKPYDSFRGP
                     DQLIIYDSSYGEFTGTNPYGYEITVNSEGIIIGTGGNDSKIPEGGYVLSGHGESMKWL
                     QKNALLGSTVTANHDKKEVTIILTPESYTSRALLNIKSAQDRLGLAKLQYLDIPYDDV
                     RKAIDVAADKLSKLNRQLKEGRYENLAGTVQEIQKVTDKAWFMTFESVKAENRAVWLR
                     PRDINIEDVKKSFDMLESININTIYLETYWNGYAIYPTGNDLMRQNPLFKGFDVLDSY
                     IKEAHSRGIELHAWVENFLVDLPIAEKKPEWMNVSRKGDTYYLENGITKYYFLNPALP
                     EVYDFLSSLYTELIEKYDIDGIQFDYLRYPNSHDYTNDFGYDTYTRQLFANFTGTDPI
                     SLMPGDSLWQEWCEFRQYIISSFACRIFSELKSIQPEIQISADVWPDYDETLSDIYQN
                     PKTWVRKDYINTLIPMSYYLHEKPVIEDLENTWSFSRGHSRITSGIATFNKVDTKVFI
                     KQIDAVRASNTSGIAIFEYESLFSSGYDYALKLGAFSTPSAVTGKNPEESINIILKDI
                     IRKLDDVYLKNNGINKNQRNKYKNLIEKLGAGFDEEQKNNIQEAIKAIYNDKNLNVEI
                     SKRIIADLNRAINIIDGHIANTRLMERLRIIAEKSDGVLKAAEISYSRVKLGWGSNAN
                     DSGIAGYILYRNGKEILRTSSYSINDNDLDFGGTYTYEVHGFDAVGNIVYRSNSLTVR
                     TKSSLLAVSSPPKNTIF"
     gene            12401..13615
                     /locus_tag="PKU88_07675"
     CDS             12401..13615
                     /locus_tag="PKU88_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPX69192.1"
                     /translation="MYGLALEGGGVRGAFHMGAYKALLEEGYEFGGVTGTSIGALNGA
                     IIAQGDFEAGYKLWESMDTPLLFDIEEEQYRKLIDRKIDRELLLRLAARMGEILENKG
                     INTSKIRQVLESIINEEKLRKSNIDFGLVTVSITDLKPLELYKENIPEGKMIDYLMAS
                     ASFPGFKLNPIGDKYYIDGAFYDNCPINLLARKGYKDIIAVRTLGLGIVQHVRYQSVK
                     VTNIFPSEDLGSPLDFNRDLIIRNLQLGYYDTMKLLKRLKGRKYYIIPEDEEKVFEIF
                     RSLPDMLVRELGKLFNTKEMTVKRMLFERIIPEIADLLGLKAASEYQDILIGLLEYLA
                     EESGVERFRIYSVKEFMKEIKFQREGKSRMHVKRPVLTGRKSLNTRLAYILKRKSRLS
                     KAAKNIFDEISS"
     gene            13749..15131
                     /locus_tag="PKU88_07680"
     CDS             13749..15131
                     /locus_tag="PKU88_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPX69193.1"
                     /translation="MYKPVAEPFRIKMVESIRMISKEERVKALKEAGYNPFALKAEDI
                     YIDLLTDSGTGAMSDYQWSGMMLGDESYAGSRNFYNLLEAVKDIIGYPYCVPVHQGRG
                     AEKVLFSNLIKKKGQYVLGNMHFDTTMAHIELNGGRPLNFITEKAFNTTEPYEWKGNF
                     DLEKLEAFIKENDRENIAFILITITCNSSGGQPVSMENIKGVHALAKKYGIRLFIDAA
                     RFAENAYFIKRREKGYENKSITEIVREMFSYAEGFTMSAKKDAIVNIGGLIGIKEDEE
                     LFTAVKTTLIPYEGFVSYGGLAGRDMEALARGLREGVNEDYLHYRISQVEYLGYRLTE
                     GGVPIQLPTGGHAVFVDAKKMLPHIPYYQFPAQVLANALYIESGVRAVEIGSFLLGRD
                     PETGEHRESPLELMRLTIPRRVYTNSHMDYIADALIYLKDKVNTLKGLDFEYEPKVLR
                     HFTARLKPIE"
     gene            15315..16262
                     /locus_tag="PKU88_07685"
     CDS             15315..16262
                     /locus_tag="PKU88_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPX69194.1"
                     /translation="MSEVKKAVYYISTNEGRPGTVSRVVWEILNDKGLLKPAGMSFDN
                     REVMMHRDGAGNEYYFAQSEVPVCWDYPRYLAEMNERFADADFSGMVTWHEGASAPPK
                     VLTVHTIGDVNAGVYGPANPAYMRNILISMEHNRVMLGLSDYMTVTEATHWSGTAVSG
                     SSPELILHYPVPMVDIEVGSEEDSWNNRTACEALAGALTHVFESDGKRVHNLLCVGGI
                     HFDPNFAQAVFTQWEDDAFGVSHIIANQWLVAGEYEKEHGLAFTSAAVDKIAGGVEAV
                     VLHDKMKGCYKDLVRAVGEKYQIPVLRHQRLRQPEELQL"
     gene            16454..16792
                     /locus_tag="PKU88_07690"
     CDS             16454..16792
                     /locus_tag="PKU88_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPX69195.1"
                     /translation="MSNQLTAVAALINDKVKFSGVSRDKKEIIIDYVKPIGDGEGYTS
                     LELFLISLATCSGTSVSSLLRKMNKDVNGLNITTYGERREEHPTYFKKYSYILNLCQR
                     MLRFLIWKNP"
     gene            complement(16859..17314)
                     /locus_tag="PKU88_07695"
     CDS             complement(16859..17314)
                     /locus_tag="PKU88_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69196.1"
                     /translation="MSKSFRKPVFSSRFNRLPDSTVEKCGECCLVHEKFSLNLIKKAT
                     NKPSTNTIFKASEFQDCSPVQIGIAQNSSRFPFGSILVRNFSAVPVSFTVFGDTEEET
                     DMTVQSNSEVAVTLNAISAVEIFYPTDPIRLLFIFDIFHPGDPITPPNP"
     gene            complement(17476..17552)
                     /locus_tag="PKU88_07700"
     tRNA            complement(17476..17552)
                     /locus_tag="PKU88_07700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17516..17518),aa:Pro,seq:ggg)
     gene            complement(17651..18007)
                     /locus_tag="PKU88_07705"
     CDS             complement(17651..18007)
                     /locus_tag="PKU88_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPX69197.1"
                     /translation="MKQRKHILKKKTMNKLYYSPKAFEDLDEIWTYIMGDLGSPAAAQ
                     NTITNILNSIEKLQEFPEMGPSLSSVTEIESNYRFLVCGICLAFYRVKGTEINIDRIL
                     YSRRDYIHILFGNTSE"
     gene            complement(17964..18221)
                     /locus_tag="PKU88_07710"
     CDS             complement(17964..18221)
                     /locus_tag="PKU88_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPX69198.1"
                     /translation="MPNIKPVSDLRNYNEVLRDVAVGEPVFLTKNGRGRYALVDILDY
                     EKAQATIKLMSELAKGQKSGEEKGWLSHEAAKAHFKEKNNE"
     gene            18494..19762
                     /locus_tag="PKU88_07715"
     CDS             18494..19762
                     /locus_tag="PKU88_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPX69199.1"
                     /translation="MRAKKNKISSVYIFPVLFFVICLSYFIRFMFFNIETEIVKYGSV
                     ENSIRVKALIIRNESTTILPSGVEIKYNVNEGERVSLGKKILEIVKNDQADENISIKI
                     KQLEDRIQEIKQSDINNNFFSKDKEKIENRIKERVSDLKSIARSGDLKKLDDVKNDLA
                     ANLYKKSLIYGEGSFFGKNLEQLQKEKSVLEGIYNNSIDVIYAQASGVVSKSLDGYEQ
                     ILNIGNIKDFKLSNIREIMSSLTADSQKTSQSTTAGIKIVDNFEWYTCSLISFDQTKD
                     IKAGKKVRLRFSELGNAEVNGEVHEVSQPEGDICMVIIRISDHVNDFYKKRIAEVDII
                     KDYNEGFEVSANAIVVKDNTRGLYVLKKGIVKFIPVAVMNEDKERCLVRNLNKEEWDS
                     KSEYEPLKIFDEVITTTERVKENQVFTDKI"
     gene            19776..20474
                     /locus_tag="PKU88_07720"
     CDS             19776..20474
                     /locus_tag="PKU88_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPX69200.1"
                     /translation="MQEIKNNIIAISRDINDICIKKGKAPDNITIIAVTKTIDPSRVN
                     YAIKCGIRNIGENKVQEIMAKYENIEENVNWHLIGHLQTNKVKYIIDKVALIHSVDSI
                     GLAEEINKRAEKAGLVKDVLVQVNVAQEETKFGIEYDETDSFAERLSGLSSIRVKGLM
                     TIAPYYDDAELARPVFRRLKEKFDMLAAAGIPNVDMKYLSMGMTNDYKVAIEEGSNMV
                     RIGTGIFGARNYDL"
     gene            20490..20948
                     /gene="sepF"
                     /locus_tag="PKU88_07725"
     CDS             20490..20948
                     /gene="sepF"
                     /locus_tag="PKU88_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPX69201.1"
                     /translation="MESNILNKVLYFMGIGEAAEEEHEEAEVSKPQIEAVGTASHRRS
                     NVVNIHTASAPMKVVLVEPSSYDEVQSICDDLRSKKPVIINFEEMDKEVAKRMVDFIS
                     GAVYVLDGTIQKISNGIVLVAPSNVDVLGNIKSSIGDENFDLEGIFSWLK"
     gene            20970..21251
                     /locus_tag="PKU88_07730"
     CDS             20970..21251
                     /locus_tag="PKU88_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPX69202.1"
                     /translation="MKFVLAATLSYFIDFLNFMILIRVLLSWFSFNPDNKIIVLIFQL
                     TDPILEPFRKLTAKLGLNTGMIDFSPIISLLFLYYIAKPLLLTLIYTFL"
     gene            21357..21806
                     /locus_tag="PKU88_07735"
     CDS             21357..21806
                     /locus_tag="PKU88_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPX69203.1"
                     /translation="MITPMDIRNKEFKKAFKGYKEDEVDEFLDKVVADYERIYRENGE
                     LKDRIAIDNDRIESYNSMEKSLQSTLLIAQTTAEDIVANARKKAEMIINEAEDQGRKI
                     IEEANSSVIKVNKDFEELKKEVQVFKTRFKTLLESELEALNATLKDI"
     gene            21906..22196
                     /locus_tag="PKU88_07740"
     CDS             21906..22196
                     /locus_tag="PKU88_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HPX69204.1"
                     /translation="MDIDNTEVKELESRIEKIALLLEKAKIGEYVNIMTKPKTLLLNN
                     FIGGVARGFGTAVGFTILGAVVIYILRQSVLLNLPFIGGFIAEIVKIVQDHL"
     gene            22218..22826
                     /locus_tag="PKU88_07745"
     CDS             22218..22826
                     /locus_tag="PKU88_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPX69205.1"
                     /translation="MDKNDFKHFEELLKDEKKKRENSLEDERTGLGISVKDLTGELSS
                     YDNHPGDMGNETFEAEKNFSFRTRDKFLLSEIDAALNRIADGTYGSCEICHNEIGKDR
                     LELRPYSRLCINCEKEYGSNVQNEEKGRPIEEQVLYPPFGRTFTDNSVEDKVQFDGED
                     AWESVNEYNVRMSDNEFGEDESAGYVEDVEQVSNEQYRKQLE"
     gene            22885..23328
                     /gene="lspA"
                     /locus_tag="PKU88_07750"
     CDS             22885..23328
                     /gene="lspA"
                     /locus_tag="PKU88_07750"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPX69206.1"
                     /translation="MYPVLIAAAIIVSDQVIKNILANALASGPITVIKNFFYLVYVEN
                     YGIAFGMFKNKVLFFIVTNCIISAAVAFMIYKYRNKSVPVTICLSLILGGAIGNVIDR
                     IRFGYVIDYLSFTIFPPVFNLADSAIVVGAILLSLLLIFGKDIKI"
     gene            23419..24357
                     /locus_tag="PKU88_07755"
     CDS             23419..24357
                     /locus_tag="PKU88_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPX69207.1"
                     /translation="MEVKEYIAGPEAEGERLDCFLSGKVEGYSRTYMQKLIEEGHCKV
                     NGKNAKPRFKLREGDRVEAVIPDPVPLEVEPEKIDLDIIYEDNDIVVINKPQGMVVHP
                     SHGNYSGTVVNALMGHCKNLSDYNSLTGINGVMRPGIVHRIDKDTSGIIVVAKNNEAH
                     LSISGQLKEHTITRKYTALLEGRLKKECGTADTLIGRNPKNRKQMAVVRENGKRAVTH
                     YRVLESFENHTLIEAVLETGRTHQIRVHMAYLGHPVVGDTVYGFKKQRFDTKGQLLHA
                     RVLGFIHPAKKEYVEFEAPIPEYFEKVLRLLRNGRL"
     gene            24497..25036
                     /gene="pyrR"
                     /locus_tag="PKU88_07760"
     CDS             24497..25036
                     /gene="pyrR"
                     /locus_tag="PKU88_07760"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPX69208.1"
                     /translation="MRIKTEIMDEKALDRALTRISHEIIERNKGVEGLVLLGIKTRGV
                     SLAKRLCEKIRNIEGNVLPVGTLDITRHRDDIKDMKDIPRENLTDISFDINGMKVVLV
                     DDVLYTGRTVRAAMDAIVEIGRPLSIQLAVLVDRGHRELPIRADYVGKNVPTSRSEMI
                     IVNVGEVDGKDAVLLGEQE"
     gene            25087..26373
                     /locus_tag="PKU88_07765"
     CDS             25087..26373
                     /locus_tag="PKU88_07765"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPX69209.1"
                     /translation="MRLLIKQCSIIDPGNTYEGINDIYIENGLIKLIEPSIGLAADKV
                     IDAEGLFLLPGFIDMHAHLREPGFEYKETVYSGTRAAARGGYTSICCMPNTRPVIDDE
                     ASLSMLQKIIKKDAAVKVYPIAAISKGQKSTELVEMNKLSEMGAVAFSDDGRPVATAA
                     FMMKALLSAKEKGFMIIDHCEELSLAEGGAINKGRKSEQMGIRGIHPLSEELNVMRDI
                     MIAGNTYSHIHIAHVSTAGSAEIIRAAKKRGIKVTCEVTPHHIGLTEDIITPGFTDCK
                     VNPPLRTAGDVEALKEALKDGTIDVIATDHAPHHKDEKSEDFYSSAFGISGLETAFSV
                     CYTELVEPGILTLKELSERMSKNPAELLKLDTGIIRTGIAADLVLVDTAREITVNRND
                     FLSKGKNTPIHGKTYKGDIVYTIVDGNVAYENKNRL"
     gene            26385..27323
                     /gene="pyrF"
                     /locus_tag="PKU88_07770"
     CDS             26385..27323
                     /gene="pyrF"
                     /locus_tag="PKU88_07770"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPX69210.1"
                     /translation="MFTDILIEKVVKCRNHTVVGLDPRLQYLPDYLIDEAVEAVAEGS
                     RASVIAEAFFRFNKEIIDNVYDIVPAVKPQIAFYEQYGTAGFECYLKTCRYAKSKGLL
                     VIGDVKRGDIGSTAEAYSDFYLGNNKASAETDCITINPYLGTDSMIPFVNNCEKNGKG
                     IFVLVKTSNKSSKDIQDLESGGKKVYEHVAELVVRLGEGLTGKYGYSSVGAVVGATFR
                     SEGQRLRELMKGVYFLVPGYGTQGGTAEDAAVCFNDDGLGAVINSSRGIIAAYKSEVY
                     RERFSEKEFGLAAREAAIAMRHDINNELAGTGKIAW"
     gene            27338..28093
                     /locus_tag="PKU88_07775"
     CDS             27338..28093
                     /locus_tag="PKU88_07775"
                     /inference="COORDINATES: protein motif:HMM:NF000798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPX69211.1"
                     /translation="MQCICKIVENRELCKDVFEMKIKCPEMVREAQPGQFLHLKVNKS
                     IDPLLRRPISISRIYRDTGVISIVYQCVGKGTKEMAALKKGEYIDVLGPLGSGFTLSD
                     EGKCAVVGGGIGIAPLLELACSLKDCDAYLGFRCNTYKLDEFSSACKELYIATEDGST
                     GSKGYVTDIIKDITKYNVIYACGPKGMLKRVKELCEDTGVKCFVSVEERMGCGIGACL
                     VCACRIKEGDTWHYKKACTDGPVFAAEEVDFDD"
     gene            28086..29027
                     /locus_tag="PKU88_07780"
     CDS             28086..29027
                     /locus_tag="PKU88_07780"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPX69212.1"
                     /translation="MTRMSVNIGGIKLKNPVLTSSGTFGFGREYSGFYDLSKLGGIVV
                     KGLTLEPREGNKPPRIAETPAGMLNSVGLQNPGVEYFIEKELPFLRGYDVAVIANIAG
                     NTVEEYCMMARRLGSLVDGIELNISCPNVKEGGAAFGISSESVYNITRRVKEQCKVPL
                     IVKLSPNVTDIKEIAESAKEGGADALSLINTLLGMAIDINSRRPVLANNVGGLSGPAV
                     KPVAVGMVWQAAQAVDIPIIGMGGISCWEDAIEFMLAGADAVSVGTANFINPYAPLEI
                     LEGIEKYLIKNQFSNINELKGNLKIYGGDNKCLQKKE"
     gene            29006..29581
                     /gene="pyrE"
                     /locus_tag="PKU88_07785"
     CDS             29006..29581
                     /gene="pyrE"
                     /locus_tag="PKU88_07785"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPX69213.1"
                     /translation="MLTKKRVNGILEKTGVLLTGHFQLTSGKHSNKYLQCAKVFQHPD
                     ISAEISKDLAEKYDGYDIDIVIGPAVGGIILAYEVARQLEVRALFAERENGIMTLRRG
                     FEIQRGSKVLVVEDVITTGGSVKEVIDVVRSAGGEVVGVAAVVDRSGGKALFDVPFKS
                     AIKIDIEVFEPGDCPMCKSGSKAVKPGSRNI"
     gene            29771..>29922
                     /locus_tag="PKU88_07790"
     CDS             29771..>29922
                     /locus_tag="PKU88_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPX69214.1"
                     /translation="MTEKRENWGSRFGFLMAAAGSAVGLGNIWRFPYLTGMNGGAAFI
                     IIYLGCV"
     assembly_gap    29923..30022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30023..30405
                     /locus_tag="PKU88_07795"
     CDS             <30023..30405
                     /locus_tag="PKU88_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPX69215.1"
                     /translation="TSAIMAVMCMLSSLSMGVMSGFTIFGVGFFDFFDILTDKIFLAV
                     GGGLLAICVGFFMKKEDLKDELTNGGTIEFGLFELWYNVTKYVIPVIIAVIAFSGIMA
                     IQQKGLMIFGLVIIAVLAAFSKKL"
     gene            complement(30452..>31462)
                     /locus_tag="PKU88_07800"
     CDS             complement(30452..>31462)
                     /locus_tag="PKU88_07800"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HPX69216.1"
                     /translation="AAFKPNIVYKGGKALDFFCFDLEIYRDLEKQEFQGMSEAVERFY
                     HEKDQKDRIKQKSGDIYRIISNRLERCFKKLERLNGDLQEAADSEKFKIYGDLIMSNI
                     YYLQRGEEKARLQNYYSSEGEYVDIPMDIRLSPSENAQKYYKHYNKSKNAIIKITEQL
                     EENKQEIMYLETQLDNLSKCTEELELEEIRAELAEQGYLKRRAAKGKQTKKTSTPMHF
                     VSSSGFEIFVGKNNMQNDYLTLKFASSQDIWLHTKDIPGSHVLIKTEGRDVDDNTLEE
                     AANLAAFFSKGRMSSKVPVDYTRRKNVKKPGGAKPGMVVYDNYFTMYITPDEEKVNSM
                     KK"
BASE COUNT         9658 a         5452 c         7458 g         8794 t
ORIGIN      
        1 ttttttgtaa atagcaaaat ttttattact ttgctttctt atgtcttgat tcgactactt
       61 caagccgctt atcctgacta ttaattgaca tagcaagtat atcaatctta ttttctatac
      121 tttctaacct ttctgtgtgt tctcccagtt taacagtatt gtcaccggtt ctttcatgca
      181 aggcttgaag ctttgcttta atgtcatgct caagagtggt ttcaatttta ctcattctat
      241 tatcaattgt acccattcta tgttcaagct tattttgtcc cgactccaga ttgtcaagcc
      301 gctgggaatt attttttacc tcattcttag tgtcctgcag ctctacatat accttttcca
      361 aaagatcata gaccttatca atatcactca tgaggtttcc tcctaaaata cattcctggt
      421 gatatttagt ttaccacaaa ccggaagaat ttagaagtaa aatcatataa gatactgtaa
      481 agaagcatgg gaagcgcagc tgcaatcgta gccgcccgcc ggctgctgac agttgcatgc
      541 aagataagtg cggtatatac aataataatt atacttcaaa catgtttttc aaaacagctt
      601 ccaggctttc accggatata accaatccat tttcagtatt ttttaagccg aaaaagtcta
      661 ctatcagttt attatcagtt ttttcaatac ctttacttaa acccggtaca accactactt
      721 caacatcttc ttttgctttt tctgcaatac tgttaaggtt cagctttttt atagcattta
      781 tggcaggctg aacctcatct atatattcca ttatcataag gctattggaa taatactttc
      841 tttcaggttt gtttcccgag aatggttctt tgttgtttaa tgcgctcaat gctcttctga
      901 gagattcgtt gttaaaatcc tcattatata tgtatgagaa ttcctgcatg gaacagagat
      961 cattcaggaa gctttcactg actttttccc tggacatcag tgaattccaa aagaagacca
     1021 tgatttcaag tccgttcaag ttgattttta tgtttgccat atcaacacct ctgtttatat
     1081 ttgttcttcc tttgtagaag acttatatta ttataactca aaactccgtt gctttccacc
     1141 ccaaccttgc ccatcaatac ctgcaggttt aatatttgtt ttgaagttat aattatgcaa
     1201 tcaaaagtaa ttctgttata ataaaagatg gtgaaggttt agtcttagga tagcaattaa
     1261 gcattagcat taccatatac aggaatatgc aaaaagagaa aaaggtattt ttagacaaga
     1321 ggtgatattt tgtggctaaa ataaagactg acattagaaa tgcagttcgc agaagctatg
     1381 gcagaattgc ctccggcagg gcaaaagatg ggggctgctg cggcagtaat atcagtgtta
     1441 aagaatctgc aaaagatata tcaagaggaa tcgggtattc cgaagaagaa atgcaggaaa
     1501 ttccggaagg cgccgacatg gggctgggct gcggaaatcc tcagctaatc gcagagataa
     1561 aagaaggcga aacagtaatt gatcttggaa gcggcggagg aatagattgt ttccttgcat
     1621 caaggaaggt aggccccaaa gggtatgtaa tcggggtgga tatgactcct gagatgttga
     1681 gtaaatcaag gattttagca aggaagcaca gatatttcaa tgtagatttc agactgggag
     1741 aaattgagaa ccttccggta gccgataata cggcggacgt gattatctca aattgtgtta
     1801 tcaacttatc ccctaaaaag cagagggtat atgatgaagt gtaccgggtg ttaaaaaaag
     1861 gcggcagaat agcaatttcc gacgtggttt tggtcaagga actttcaaat gaaatgaagc
     1921 aggatgaaaa gctttactgc gggtgagtcg ccggtgcttc ttcagttgaa gaactcaagg
     1981 catatttaga gaaagccgga ttcagtaata ttaatattga tactaaagag gtatctgagg
     2041 aatatgccgc aaaatggtcg cacaacttaa aagtcggaga gtatattatg tccgcatcga
     2101 ttaaggcggt taaggctaaa tgaatgaagg aatcaaggag gtattgaaat gcctgaatta
     2161 agcagcaagg taaatatatt taccgattca gtcataagac gtatgaccag gatttccgag
     2221 gcctacgggg caataaacct ttcacagggg tttcctgatt ttgacccccc caaggagatt
     2281 ttggatgcat tggcttatgc agcatataac ggccctcatc aatatacatt tacatacggt
     2341 tcctacaatc ttagaagcgc tttggcaaaa aaacacggga aagctattaa tcgaaccatt
     2401 gatcccgata tggaggtgct ggtcacctgc ggcggcacgg aagcaatgat gagcgctatg
     2461 atgacaatct gcaatccggg ggataatgtt attatattca gcccctatta tgaaaattac
     2521 agagcggaca cgattctgtc gggggcaaac ccgataatag ttcccttgaa tcctccggac
     2581 tatagttttg acaaacaggt tttggaagat gctttccgga ataatgtgaa ggcaatcatt
     2641 gtatgcaatc cgtccaatcc gacgggtaag gtatttacaa cagaagaact ggagtttata
     2701 ggacatttgg cattgaggta tgatgcatat gtaattaccg atgaagtcta tgagcatata
     2761 atatacgaac cttttcatca tgttcatatg gcaggccttc ctgatatgtt cgaacatacg
     2821 ataacctgca gctccctgtc caaaacctat tctattacag gctggcgcct tggatatctg
     2881 attgggccgg aaaaggtaat tgaaagcgct aaaaaagttc atgattttct gacgatatgt
     2941 gctccggcac cgctgcaaga agctgcatgc gtcggtgtaa actttgggga gtcatattat
     3001 aaagatcttc aaaggaaata taccgaaaag cgaaattatt tctgcaggcg cctggatgaa
     3061 ataggtttac ggcacaatac gcctcagggc acttattttg ttatgatgga tatttcagaa
     3121 ttcttatcaa tggataagtt taagggatgg actgacctgc aattctgtga atggatgatt
     3181 atggagatcg gtgttgctgc ggtgccgggt tcaagctttt tcatggaacc tgtcaataat
     3241 ttgatacggc tgcattttgc aagaagcatg gatactttgg acgaagtgtt gaaccgtctt
     3301 gaaaaattag gccggtatct gcacagatag aaaaaatata cgtggtatgc agggaggaaa
     3361 accggatgag agaacttaat gttgatgtaa tcaccaatac tgttgagaat ttatgtatcg
     3421 aatcaaatta ctttcttccc cgggatgtaa aaaaagccct tgaagatgcg gtcctgaagg
     3481 aggaatcgcc tgttggaagg gaaacgcttc ttgatattct taagaatgcg gaaatagctg
     3541 caaggaatca agtgcctatt tgccaggaca caggcttgac agttgttttt gctgaacttg
     3601 gccaggactt gagactggtt ggaggagatt tttatgaagc gattaacgaa ggtgtaaggc
     3661 gcgggtataa aaacgggtat ttgaggaagt ctgcagttga tgatcctttt ctggtgagaa
     3721 agaataccgg cgacaacaca ccggcaatta ttcatacaac cattgtaaag ggagacaggt
     3781 taaagattat tatagcgcca aaaggaggag gaagcgaaaa catgagtgcc cttcgtatgc
     3841 ttacgcctgc cgaaggagtc gacggaatca agaaatttgt aatagatacg gtggataagg
     3901 cgggttcaaa tccgtgtccg cctgtaattg taggtgtagg tatcgggggt actgtcgaaa
     3961 tgactgcatt gattgccaaa aaggctttat tgcgcactgt aggagaacat aatcccaaca
     4021 ctgaggttgc caaactggag aatgagctgc ttgaggaaat caacaatctg ggtataggcc
     4081 ctcaaggctt tgggggcagg attacagccc ttgcagtaaa cattgagacc tttcccgccc
     4141 atttggcaag catgcctgtc gcagtaaata tccagtgtca tgccgcaagg cataaggaag
     4201 cagtactata agcgaagcag gaggtgcaaa agagtgtcca ggattataaa tacacctttg
     4261 acagatgagg tgacatgcaa tttacatgcg ggggacaggg tgcttataaa cgggattata
     4321 tataccgggc gtgatgctgc tcataaaaga ttgactgaat tgctggatcg gggagaaaaa
     4381 ctgccctttg agcttaacgg gcaggttatt tattatgtcg ggccttgtcc cgcaaagccc
     4441 ggtaatgtaa taggctctgc aggccctaca acgagctgcc gcatggatgt ttatgccccc
     4501 gggctgatgg aaatgggcct caaaggcatg attggaaaag ggtcacgcag taaagatgta
     4561 attgattcca tagtcaggaa taagtgtgta tacttcgcgg cagtcggcgg agcaggggct
     4621 ctgcttgcag aagctataaa ggaagcagag gttgtggcat ttgcagacct gggccccgag
     4681 gcaatattca aattgaaagt ggaaaatttc ccggccgttg taatagcaga tacggaagga
     4741 aaagatttgt acaaaatcgg aagaagtaaa tatcaaagat aatatggagg aatgattatg
     4801 tcattaaggg aagatgcact taaacttcat agggataaca ggggaaagct tcaagtagta
     4861 agcaaggtgt cgtttaatga taaagaaaac ctgagccttg catatactcc gggggttgca
     4921 gagccttgta aggagattca aaaggatccc gataaagttt atgaatatac tttcaagggc
     4981 aatatggtgg cagttgtaac agacggcacg gctgtcctgg ggctggggga tatcggcagc
     5041 catgctgcct tgccggttat ggaaggcaag gccgttttat tcaagaattt cggcggagtt
     5101 gatgctttcc cgatatgcct taataccatc gatgcagata agatagttga atcagtaact
     5161 ctgatagagc ctgccttcgg aggaataaac cttgaagata tagctgctcc ggcatgcttc
     5221 gagatagaaa acaagcttaa gaagaagctt tcaatacctg ttttccatga tgatcagcac
     5281 ggaactgcaa tcatagtgct tgcaggcctt ctgaattcca taaagattac cgggaaaaag
     5341 cttcaggagc ttgctgttgt cataaacggt tcaggtgcgg ccggtattgc aattaccaag
     5401 atattgctca gcgccggagt taaagatgcc attctatgtg acagagccgg tattatttat
     5461 aaaggaagaa atacaggcat gaattgggct aaagaggaaa ttgcccttgt aacaaacaaa
     5521 gaatgccgaa aaggcacatt ggcagatgct atggtgaatt cagatgtgtt tgtcggagta
     5581 tcctcagcca atatcgtaag tgaagacatg gtcaggagta tgaacaaaga cgctgttatt
     5641 tttgcaatgg caaatcctgt tccggaaata gatcctgaac cggcaaaaaa agccggagcg
     5701 cgtattatcg gtacgggaag atcggactac cccaatcagg taaacaatgt actggcattc
     5761 ccgggtgtat tcagaggcgc tcttgatgta cgtgcatccg agattaacga ggaaatgaag
     5821 ctggctgcag cttatgcact ttctgaaata attccggccg atgaattgac ggaggattat
     5881 ataattccaa aggtttttga tccgagagtt gccccggctg ttgccaaagc agtagcaaag
     5941 gctgcaatag aaacaggagt tgcaagagcg gcagtagatc ctgaacaggt tgccttaagg
     6001 gctgcggggt taataaaact aaaataaaag ctgatgtgaa ggaagtgtac tatggttata
     6061 aaacagacta ttatacgtgg aatcaataat gccttaaagg tcattctgga tcttataaaa
     6121 tacattattc ctgcagtttt tgttctgaag gtattggagc attcgggatg gctcgtcagg
     6181 attgcggacc tttttggacc gtttatgagc tatataggat tgcccggaga aggagcactg
     6241 attattgtga tggggcaggt aacattatat agtgcaattg ctgctatggc tgtcgtggac
     6301 attacagtaa agcagttaac cattgtaagc acctttgtta ccatatttca ttcattcata
     6361 ttggagacag cggtgataaa caaagcaggc ggaaacgggt tcatgataat aggaataagg
     6421 gttattgcag cagtactggt atgcttcata ttgaatatat taatcccggg ggtatagaag
     6481 atgaattggg cggaagtttt tatggaaaca tttgaaatca gtttttcaac aatatttaag
     6541 atgacggtta ttgtagttcc tttgcttata ggcatcgaat atctcaagga aataggatgg
     6601 atggagaagc tgtccgtaaa gcttagaaag gtttcggagc ttatagggct gccgggagaa
     6661 gccgcttttg ggatgatagt agtatacttc atcggactgg ttttcggatc gggagtaata
     6721 attaggatga aaaaagaggt tggaatgaca aggaaacaac ttaataccat gtttatattt
     6781 attggcatat gccatgccgt tgttgaagag accatcgtct ttacagcagt tggtgcaaat
     6841 ggggtaatag tgatggtgag cagggtgctt gcttcatttt tatttggctt gttgtatgct
     6901 tggtttgcta aacatgacga aacttccata aggaaaagct ctggtattga gcttgagtga
     6961 aaaaatatgt tattataatt ttaattaaat ttgcaggagg atgttacagt gaaaaaaggc
     7021 agtattatat ggatcattgc gttgctgttg tggatattgg ttctggcggt tccggctacc
     7081 cggtcgacat ttatggctgc taccgacgcc catccttatg taatgggatt catcaagttt
     7141 gccatactgg caactatggg agatttgctc ggaataagag tcctcggagg agaatggata
     7201 attccgagag gggtttttta taaatctata atctggggta tcatcggaat gatgaccacc
     7261 cttgtattta cggtatacat ggggggcact tcggcagctc aggcagcagg gagactgccc
     7321 ttcaaggaat ccgctctggc cttggctttt tttacaagtg caattatgaa tcttaccttc
     7381 ggacctataa tgatggcttt tcacaggttt acagacatgt atatcgacag caggtatgaa
     7441 agcagcaaag gcagaactac aataagcagc ctgattgaca ggaacgactg gcattctctt
     7501 gtcgaattca gctggcttaa gacatgccct tttttctgga tacctgccca tacaatagta
     7561 ttcatgctgc ctgctcaata cagagtcctt atttctgcat ttctatcaat tgcactgggc
     7621 ctgttattgg ctctggctaa aaaaggaaag gccgcaaagg cggcataagg agggaatgtt
     7681 tatgagaaga gttcttgcga tagttgcggc ttcaattctg attctgcttg cagcaggatg
     7741 tataaaggaa gccgatacat acctatataa gggcagagac ggggactgga gcatagattt
     7801 gcagaaggag tttaccaaga gtaaggagga gtacgacgaa gaactgaaat cttatacaat
     7861 taccttcaag acagaaagtg atttattttt tgtaattaat gaaataattg acgaaaagct
     7921 tgaaatcaac caggacagaa ttaaggaaga gttaagcctg gatgattata tgaaagcaga
     7981 gagatatgat aatatagaca tacctgatat tggaacggct tatggggcgc tggtgttcga
     8041 cgaagctacc ggtacggcca tgatgtatca caggctaaga tataaggata aggtgatttc
     8101 ctttatattt cattacaaga acgatttatc cgatgagcat gaagctaaag caaaagcaat
     8161 catcggttca ttcaaaggat taaaatagga ataacggctg cgccgttatt ttttattgta
     8221 taggaaagta tgctttccta tacaatgggg gatgtacgcg ttttttatca taaaagttga
     8281 tataatgtgc aaggatattt actgaagaaa aatagatatg gctaatgaag gtgaaaatgt
     8341 gaacaggaaa aggcttattg aagcattttt aaagattttt ctatgggtgg ttgcagtaat
     8401 aataaatata atgtatattt caggattgtt tgaacatctg ccggaggagt taaagccatt
     8461 cataattaca ggagatgtgt ccatggtagt aatactatta tggttcatgc cctcaacaat
     8521 ggcggaaaga aggctgaaga agggacttga atacctggat cgggataacg acaaggctgt
     8581 aaggcatatt gaagcatacc ttgattcaaa aatgcttact atgaatgaaa taaagcatgc
     8641 cttgagagta ctgggagttg cccaccataa aagaggggat gatgcggcgg ctattaaatg
     8701 ccttaccaag gctcttgagg gaaactataa ggataatgac ctgaaggcag agatcctggg
     8761 tgcaatgggg ataatatatt ccgagtctgg agagttccaa aaggcagcgg aatgtttcga
     8821 caggactttt gaaatcatgt ttgccatgtc caaagcaaat atcgacaagg ctatatttat
     8881 tcaggttata aatacttata taaaaacagg ccaaaaggaa aaagcactga tgatatatga
     8941 caggctcctt atgattcagg gattcacacg ggataagaga gtggaggaga tattgggtat
     9001 ataaaaggaa tgctaaggtt cttaatatac tatcatcctc ttttggtata gtataaatga
     9061 aggaaaacag ggctggcgta gaatatagga ttaagcaaaa gcaatgatta tagttattat
     9121 ataaggagga gaaatatgaa caagagatgg aacgtgtttg taactaggat gctgcctcac
     9181 attgggagtg ctactataga agcgagaatt aacatgggta tgatagcggc aagaaatatt
     9241 atagctgcta tgaatggcaa agtcccgaag acgcttgtta atacggacgt tcttaaaaaa
     9301 tagtattctt aggagggctg ctgacggcaa gaagcgatga ctttgttctg acggtcagac
     9361 tgttgcttct gtatacaatg tttcctactg catcaaatcc gtgtacctca taggtgtaag
     9421 tcccaccgaa gtccaagtca ttatcattta tggaataaga agaagttctg aggatttctt
     9481 tgccattgcg gtacaaaata taacctgcta tgcctgagtc atttgcattg cttccccatc
     9541 caagcttcac tcttgaatag cttatttcag cggcttttaa aacaccgtcg gatttttcgg
     9601 caattattct aagtctttcc ataagccttg tatttgcaat atgcccgtcg ataatattta
     9661 ttgcgcggtt caagtcagca atgattcgtt ttgagatttc cacattgaga tttttatcat
     9721 tatatatagc ctttattgct tcttgtatat tgtttttttg ttcttcatca aagcccgctc
     9781 ctagcttctc aataaggttt ttgtatttgt ttctctgatt tttgtttata ccattattct
     9841 tcagatatac atcatcaagc tttcttatta tgtcttttaa aattatattt atcgattctt
     9901 ccggattttt acccgtaact gccgaggggg tgctgaaggc tccgagcttc aatgcataat
     9961 catagccgct gctgaacagc gactcgtatt caaagattgc aatgccgctt gtatttgaag
    10021 cccttacagc gtcaatttgt tttataaaca ctttggtatc aaccttgtta aacgttgcta
    10081 ttcctgaagt gatccgggaa tggccccttg agaaggacca ggtattttct aaatcttcaa
    10141 tgacaggttt ttcatgcaga tagtaggaca taggtatcag ggtattgatg taatcctttc
    10201 taacccatgt ctttggattc tgatatatgt cggagagggt ttcatcatag tccggccata
    10261 catcagcgga tatttgaatt tcaggctgta tcgattttaa ttctgaaaat atcctgcaag
    10321 caaaggaact aataatatac tgtctgaatt cacaccattc ctgccataat gagtcccccg
    10381 gcatcagtga aattgggtca gtaccggtaa aattagcgaa cagctgcctg gtatatgtat
    10441 catagccgaa gtcatttgta taatcgtggg aatttggata tctcaagtag tcaaattgaa
    10501 taccgtcaat atcgtatttt tcgattagtt ctgtatacag cgatgatagg aaatcgtaaa
    10561 cctcaggcag ggcgggattc aaaaagtaat atttggttat tccgttttcc agataataag
    10621 tatcgccctt tctgcttaca ttcatccact caggtttttt ctcagctatg ggcagatcca
    10681 caagaaagtt ctccacccat gcgtgaagtt caattcctct cgaatgcgct tctttaatat
    10741 atgagtccag gacatcaaat cctttgaata aaggattctg cctcatcaag tcattgcctg
    10801 tcggatatat ggcatatccg ttccagtagg tttcaagata tatggtattg atgttgattg
    10861 attccagcat atcaaaactc ttttttacat cttcaatatt tatgtctctg ggccttaacc
    10921 atactgccct gttctctgcc tttacagact caaaggtcat gaaccatgcc ttgtccgtaa
    10981 ccttttgtat ctcttgaacg gtgccggcca agttttcata ccggccttcc ttcagttgcc
    11041 tgttaagctt tgacagcttg tcggcagcaa catctattgc tttccgcaca tcatcatatg
    11101 gaatgtccag atactgaagc tttgccaggc caagcctgtc ctgtgccgat ttaatattca
    11161 ataaagccct gcttgtatat gattccggag taaggattat agttacttct tttttgtcat
    11221 ggtttgctgt aaccgtcgat cctaataagg cattcttttg aagccatttc atgctttcac
    11281 cgtgtcctga aagtacatat cccccttcgg gtatttttga gtcattgcct cctgtgccaa
    11341 ttattattcc ctcactgttt accgtaatct catagccata agggtttgta ccggtaaatt
    11401 caccgtagct gctgtcatag attatcaatt gatccggccc tctgaagctg tcatatggct
    11461 tttctttcat actgtcccat gcggcggggt tatctgccaa tgttcttggg ttgtatgcgg
    11521 catatttgat tttacttgca ttatacagct tagcatcagc tgatatatta atattaatac
    11581 ccggttttgc tttttcccgt acctgcatgt agtatgggct tcctgcgtgt attgatatta
    11641 ccgcaccatc cgtcggtatc tgcgaattat tccggccctg cagcccttcg gtatttgtca
    11701 tatctgcaat tttggtgata atgccgtctt caaccgtcag ttccattccc cacgggtttg
    11761 tctttgtcga ggtattgtaa gcagcattat ataatattat ttgattggca tcccgtactg
    11821 tattgatttg atcaattggt ataagcatat cctccagtat aaaatacata tcgggagaaa
    11881 cggggatatc aatgttggta agtgttaatc ctgtacccac ggaaaatttt cttctaaaat
    11941 cctcattgtc gccggtatat gatattacgc atccgttcgg aggaatataa gtaccattgg
    12001 cgccgttgct gttttcttga acaacaatat tgtttacaat gacgaactca tgggtatgtg
    12061 ctccgaaagg cctggtaaat tcaccatatt tgttggtata caatatgact tgttctccat
    12121 caatcgggta tgattcagag ctgtcttcga atataatcga aggagtgatg ctgatatcat
    12181 tgttttcggc ataaatattg atttgaaata aagacattaa aactaagaat atacatatca
    12241 ggctgcttac aagatgcttc atatcattgc tcctttattt tcaacatttt atgtcatata
    12301 aattatagca gagcatgtat atgtgaagca actgaagcat attaaaatag cttgtctggc
    12361 aagccacaaa atgatatatt attcaagggg aggagttttt atgtatggtc ttgcacttga
    12421 aggcggaggt gtgaggggcg cttttcacat gggtgcttat aaagccttgc tggaagaagg
    12481 atacgagttc ggaggagtga caggtacatc cataggagca ctgaacggag ccataattgc
    12541 ccagggtgat ttcgaggcag gatacaagct atgggaaagt atggatactc ctttattgtt
    12601 tgatattgaa gaagagcaat acagaaagct gattgacaga aaaatagaca gggaattgct
    12661 gttaaggctg gctgccagaa tgggcgaaat acttgaaaat aagggcataa ataccagcaa
    12721 aatacgccag gtgcttgaaa gcattataaa tgaagaaaaa ctccggaaat caaatattga
    12781 cttcggcctg gtaacggtgt cgattactga tttaaagcct ttggagctgt ataaggagaa
    12841 tattcccgaa ggaaagatga ttgattacct tatggcaagt gcaagttttc ccggattcaa
    12901 attaaacccc atcggggata aatactatat tgacggagcc ttttacgata attgccccat
    12961 taatctgctg gccagaaagg gatataagga tataatcgca gtcagaaccc tgggattggg
    13021 cattgtccag catgtgcgat atcaaagcgt gaaggtcaca aatatattcc cttctgagga
    13081 cctgggaagc cccttggact tcaaccggga tctgataatt cgcaatcttc agttgggtta
    13141 ttatgataca atgaagctgc tgaagcgcct taaaggaagg aagtattaca ttatacccga
    13201 agatgaggaa aaagtatttg agattttccg cagtttgcct gatatgctgg ttagggaact
    13261 tggaaaactg ttcaatacta aggaaatgac tgtaaaaaga atgctgtttg aaagaataat
    13321 tccggagatt gccgacttat tgggattgaa agcagcctcg gagtatcagg acatactcat
    13381 tggcctgctg gaataccttg ctgaagaaag cggggtggaa agattcagga tatattcagt
    13441 gaaagagttc atgaaggaaa taaaatttca aagggaaggt aaaagcagaa tgcatgtcaa
    13501 aaggccggta ttaacgggca gaaaaagcct gaacacgaga ttggcatata ttcttaaaag
    13561 aaaaagcagg ttgagcaaag cagcaaaaaa tatatttgat gaaatcagct catagcagac
    13621 ggaaaggaga gtgagcagaa agtacttttt taaggaaact tttccgcata tcctattggg
    13681 acaagacggc ctgatttgca tcagcaagga tccagtatca ttattctata ataaaagggg
    13741 gaattgaaat gtataaacca gtagcggaac ctttcaggat caaaatggta gaatccatca
    13801 gaatgatttc caaggaggaa agagtaaaag cattaaaaga ggcaggatat aatccctttg
    13861 ccttgaaggc cgaagacata tatatcgacc tgttgacaga cagtgggacg ggagctatga
    13921 gcgactatca gtggtcaggc atgatgttgg gagatgaatc ctatgccggg agcaggaatt
    13981 tctataatct tctggaagca gtaaaagata ttattggcta tccgtattgc gtaccggtac
    14041 atcagggcag gggagcggaa aaagtattgt tcagcaatct cataaagaaa aaaggccaat
    14101 acgtacttgg gaatatgcat tttgatacca ctatggctca tattgagctg aacggcggaa
    14161 ggcctttgaa ttttattact gaaaaagcct tcaatacaac ggaaccttat gaatggaaag
    14221 gtaatttcga ccttgaaaag ctggaagctt ttattaagga aaatgataga gaaaacatcg
    14281 cttttattct gataaccata acctgcaaca gctcaggagg ccagcccgta tccatggaga
    14341 acataaaagg ggtccatgct ctcgctaaaa aatatggcat aaggctgttc atagatgctg
    14401 caaggtttgc ggagaacgct tattttataa agcgcaggga aaagggctat gaaaataaga
    14461 gtatcactga gatagttcgg gaaatgttct cttatgccga aggctttacc atgagcgcca
    14521 aaaaggatgc catagtaaat atcggaggcc ttatcggcat aaaggaagat gaagagttat
    14581 tcaccgccgt aaaaactact ttgatccctt acgagggatt tgtatcatac gggggtcttg
    14641 caggaagaga catggaagct ctggcaagag gcctcaggga aggtgtaaat gaagattacc
    14701 tgcattacag gatatcccag gttgaatacc tcggttacag gcttacggaa ggaggcgtcc
    14761 cgatacaact cccaacagga ggccatgcgg tattcgtaga cgcaaagaaa atgctgccgc
    14821 atataccgta ctatcaattc ccggcccagg ttctggcaaa tgctttgtat atcgaatcag
    14881 gagtaagggc agttgaaata ggatccttcc tgcttgggcg ggatccggag acgggagaac
    14941 acagagaatc gcctctcgaa ctaatgaggc tgacaatacc cagaagagtg tatacgaaca
    15001 gccatatgga ttatatcgct gatgccctta tatacttaaa agacaaggtc aatacattaa
    15061 aggggctgga ttttgaatat gagcctaaag tattaaggca ctttactgca aggctgaagc
    15121 caatagaata gcgaggataa ttatggagaa aacaagggcc ataatcaatc cttgaagtca
    15181 atgtatcctt ccttgtagac cactacaaaa ggattaggct gaaactagct gtttgggctg
    15241 ctgcgaaatg atacattttg cagcagcctt gtattgtacc gttccggaac ggtacagcag
    15301 aaaggagaca taatatgtca gaggtcaaga aagcggttta ttatatcagc acaaacgaag
    15361 ggcggccggg cactgtctca cgtgtcgtgt gggaaatatt gaatgataaa ggcttattaa
    15421 aacctgcggg aatgtcattt gataaccgcg aggttatgat gcatcgtgac ggggcaggta
    15481 atgaatatta tttcgcacag tcggaagttc ctgtttgctg ggattatccg agatacttgg
    15541 cggagatgaa cgagagattt gcggatgcag atttttcggg catggtcacc tggcacgagg
    15601 gggcgagtgc accgcctaag gtgcttacag tccatactat aggagatgtg aatgcgggag
    15661 tatatggacc ggctaaccct gcttatatga gaaacatact gatttcgatg gaacataacc
    15721 gcgttatgct ggggctttcg gattacatga cggttacaga ggcgactcat tggtcgggta
    15781 ctgccgtaag cggaagttca ccggaattga ttttgcatta tccggtgcct atggtggata
    15841 ttgaggtagg aagcgaggag gacagctgga acaaccgtac agcctgtgaa gctctggcag
    15901 gcgcattaac ccatgtgttt gaaagtgacg gcaaacgcgt gcacaacctt ctttgtgtag
    15961 gaggtatcca tttcgatcca aactttgcac aagccgtatt tacacaatgg gaagatgatg
    16021 cattcggtgt ttcacatatc atagccaacc agtggctcgt tgcaggggag tatgaaaagg
    16081 agcatgggct tgcatttaca agtgctgctg ttgataaaat tgcaggcggc gtggaggcgg
    16141 tggtgctcca tgacaaaatg aaaggctgct ataaggattt ggtgcgtgcg gttggagaga
    16201 agtatcagat tcccgtcctg aggcatcagc gtcttagaca gcctgaggag cttcaattgt
    16261 aaaaaaggtt ttactattgc tgaaggagag aattctatga gagaacaatg cctgcagctt
    16321 tttttgaaaa tattaaaact atgatttcta aaaaatcttt agaaatttta tcgtaataat
    16381 ctataatcga tgtgaccttt tgttattttt agtatctaag cttataaatc agaaaataac
    16441 aggaggtttt tttatgagca atcaattaac agcagttgca gcacttataa atgataaggt
    16501 gaagttctcg ggtgtttcaa gggacaaaaa ggagataatt atcgactatg taaagcctat
    16561 cggagacggt gaaggatata cctcccttga acttttcctg ataagccttg ctacctgcag
    16621 cggcacatca gtctcttcac tgttaaggaa gatgaacaag gatgttaacg gactaaatat
    16681 tacaacctac ggagagagga gggaagaaca cccaacctat tttaaaaaat actcatacat
    16741 tttgaactta tgtcaaagaa tgctgagatt cctgatatgg aaaaacccat aaaacaagcc
    16801 tgtaattgtt gcgattacag gcttttaact tacgattata ttcagggtga acgtcttatt
    16861 atgggttcgg aggggtaatt gggtcaccag gatggaatat atcaaatatg aacagcagtc
    16921 ttatagggtc agtagggtaa aatatctcta ctgcacttat agcgtttaat gttactgcaa
    16981 cctcagaatt tgattgaact gtcatatcgg tttcttcttc cgtatcccca aatactgtaa
    17041 aggagacggg tactgctgaa aaatttctta ccaatatact gccaaatggg aaacgggaag
    17101 aattttgtgc tataccaatt tgtaccggag aacaatcttg aaattcactt gccttaaaga
    17161 ttgtatttgt acttggttta tttgttgctt tttttatcag gtttagggaa aacttttcgt
    17221 gaactaaaca gcattcaccg catttttcta ctgtactgtc cggcaaccgg ttgaaacgag
    17281 atgaaaaaac tggttttcta aatgatttac tcatttatac acctcctttt gggatacctt
    17341 gtctgctttt cttaatatat agtattaagg cagtatatat ttggtgcgtt tgtggttata
    17401 aaaagtcttg acaagcctga tacagctttt tatacaaaat aaaaaaagtg ccttaaagca
    17461 ctttgattac ttagatggtc tgggtgacag gaattgaacc tgcgacctct tgaaccccat
    17521 tcaagcgcgc taccaagctg cgccacaccc agaaaaatat tcttatatca tttctgacac
    17581 ctattgaata tatcataaaa aagtataaat ttcaagaaca tattttgccg gtttaatgtc
    17641 tgcttatcgc ttattcagat gtattgccga acaagatatg aatataatcg cgtctgctgt
    17701 ataatatacg gtctatattt atctctgtac ctttaacgcg gtagaaggca agacagatac
    17761 cgcaaactag aaaacgataa ttactttcaa tttctgtaac tgaagagaga gaaggtccca
    17821 tttccggaaa ctcctgcagt ttttctatgg aattcaatat gttggttatg gtgttttgag
    17881 ctgcggcagg gcttcccaag tcgcccataa tgtatgtcca tatctcatcg agatcttcaa
    17941 aagcctttgg agagtagtat agcttattca ttgttttttt ctttaaaatg tgctttcgct
    18001 gcttcatgtg aaagccatcc tttttcctca cctgattttt gtccctttgc aagctcagac
    18061 atcagtttga ttgtcgcttg agctttttca tagtccaaaa tatcaacaag tgcatagcgc
    18121 cctctgccat tctttgttaa aaagaccggc tcgccaaccg caacatctct taagacctcg
    18181 ttataatttc gcagatcaga aactggtttg atgtttggca taatatcacc tctcttcttg
    18241 ttgataatat tatatacaaa ttcttcgtaa aatacaacat caaaaacaac agcacgcaat
    18301 attaagttgc atattgatta ttattcacac attgcattat taccttcagg actatttcaa
    18361 tgggaagctg gcactaattt tattatttgc ttaatttatg caggattatg ggaaccggtg
    18421 gagaaatata taccttatgt aaatataaag ttcaataagt tatacctgta tcttgcgagg
    18481 agctgctgtt gctatgaggg caaaaaaaaa taaaatatca tctgtttata tattccctgt
    18541 attgtttttt gttatttgtc tatcatactt tattagattc atgtttttca atattgaaac
    18601 tgaaatagta aaatatggct cagttgagaa ttcaataaga gtaaaggcat tgataataag
    18661 gaatgaatca actacaatac ttccgtcggg agtggaaatt aaatataatg tcaatgaagg
    18721 cgaacgggtc tccctgggga agaaaattct ggagattgtg aagaatgatc aggcggacga
    18781 aaatatatca ataaagataa agcaattgga ggacaggata caagaaataa aacagtcgga
    18841 tattaataac aacttttttt caaaggacaa ggaaaagata gagaaccgga taaaggaacg
    18901 ggtgtccgac cttaagagca tcgccaggtc aggagacctt aaaaagcttg atgatgtaaa
    18961 aaacgacctt gccgccaatt tatacaaaaa atctttgata tacggagaag gaagcttttt
    19021 cgggaagaat ctggagcagc tgcaaaagga aaaaagtgtc ctggagggaa tatacaataa
    19081 cagtattgat gtaatatatg cccaggcttc aggagttgta agcaaaagcc tggacggtta
    19141 tgagcagata ctgaatattg ggaatatcaa ggatttcaag ctcagcaata tcagagaaat
    19201 aatgagttct ttgacggctg acagccaaaa aaccagtcag agcactacgg ccggaattaa
    19261 gatagttgac aattttgaat ggtacacatg cagtttaata agcttcgatc agacaaagga
    19321 tataaaggca ggaaaaaaag tcagactgag attttcagag ctggggaatg cagaggttaa
    19381 cggcgaggtg catgaggtat cccagccgga aggtgacata tgcatggtaa taatcaggat
    19441 tagcgatcat gtcaatgatt tttacaaaaa gagaattgcc gaggttgata taataaagga
    19501 ttataacgaa ggcttcgaag tgtcggcaaa cgctatagtg gttaaggata atacgagagg
    19561 gctgtatgta cttaaaaaag gaattgtaaa attcatacct gttgctgtta tgaatgaaga
    19621 taaagaaaga tgcctcgtta gaaatcttaa taaggaagaa tgggattcca aatcggaata
    19681 tgagcctctg aaaatttttg acgaagtaat tactacaaca gaaagggtta aggagaatca
    19741 ggtttttacc gataaaatat aggaggggtt taactttgca ggaaatcaaa aataatatta
    19801 ttgccatatc ccgggatata aatgatattt gtataaaaaa aggcaaagcc cctgacaata
    19861 ttaccataat agccgtgaca aaaaccatag atcccagccg tgtgaattat gcaataaaat
    19921 gcgggattag aaatatcggt gagaataaag tgcaagagat aatggcaaaa tatgaaaata
    19981 ttgaagaaaa tgttaattgg cacctgattg ggcatttaca aaccaataag gtaaaatata
    20041 taatagataa agtagcattg attcattctg ttgacagcat aggcctggcg gaagaaataa
    20101 ataagagggc tgaaaaggct gggcttgtaa aagatgtgct tgtacaagtg aatgtagcac
    20161 aggaagagac caaattcgga atagaatatg atgaaacaga cagctttgca gagaggttat
    20221 cagggctttc cagcataagg gtgaaggggc ttatgaccat tgccccatat tatgacgatg
    20281 ctgagctggc aaggcctgtg ttcaggcggc tgaaagaaaa attcgacatg ctggcagcag
    20341 ccggtattcc aaatgtagat atgaagtatt tatccatggg tatgaccaac gattataagg
    20401 ttgcaattga agagggttcc aacatggtaa ggattgggac aggtattttt ggagcaagga
    20461 attatgattt ataataagga ggtatagtaa tggagtccaa tatcttgaac aaggtacttt
    20521 attttatggg aataggagaa gctgctgaag aagaacacga agaagctgaa gtatcaaaac
    20581 ctcagataga agcagtgggc accgcttccc accgcaggag caatgtagtt aatatacata
    20641 ctgcctctgc acccatgaag gttgttctgg tagagccttc ttcctatgat gaagttcaat
    20701 ccatctgtga tgatcttaga agcaaaaaac ctgtaataat caattttgaa gagatggaca
    20761 aggaagtagc caaaaggatg gtcgatttta tcagcggcgc ggtatatgta cttgacggta
    20821 caattcaaaa gatttcaaac ggtatagtgc tggttgctcc gtcaaatgtg gatgttttgg
    20881 gtaatattaa gagttctata ggcgatgaga atttcgacct ggaaggaatt ttcagctggc
    20941 ttaaataatt gtagaatgag agtggttaga tgaaatttgt cttggctgca actttgtcat
    21001 attttattga ttttcttaac tttatgatac tcattagagt tttattgtcc tggttcagtt
    21061 ttaatcccga caataagata atagtgctga tatttcagtt aacagatccg atacttgagc
    21121 cctttagaaa acttaccgcc aaattgggac tcaatacggg catgattgat ttttcaccca
    21181 taatatcatt gttatttctt tattatatag ccaaaccttt actgctgact ttgatatata
    21241 ctttcctgta aataatggtt ctgtgaagaa taatcctgga gcagtatacg atacaacaaa
    21301 aaaaggaaga ataggaattg ttattaagaa atacctgtga tggaggttga aggaaaatga
    21361 taactccgat ggatataaga aacaaggaat ttaaaaaggc cttcaaagga tacaaggaag
    21421 atgaagttga tgaatttctg gataaggtcg tagcagacta tgagagaatt tacagggaaa
    21481 acggtgagtt aaaggacaga atagccatag acaatgatag gatagaaagc tataacagca
    21541 tggaaaaatc tcttcagagc actcttctga tagcacagac aactgctgag gacattgttg
    21601 caaatgcgag gaaaaaagcg gaaatgatta ttaatgaagc agaagatcag gggagaaaga
    21661 taatagaaga agcaaacagc agtgttataa aggttaataa ggattttgag gaattgaaaa
    21721 aggaagtaca ggtctttaag accaggttca agactctgtt ggagtctgag cttgaggctc
    21781 ttaatgcaac cttgaaggat atataaagat taaaaagtga atatttatgt aggaatgttg
    21841 ataatataaa gtcatgaagt tattcatggc tttatatttt cattgggggc caagggggca
    21901 tgtgtatgga tattgataat accgaagtga aggaactgga aagcaggata gaaaaaatag
    21961 cgctactgct ggaaaaggcc aaaattggcg agtatgtcaa tattatgacc aagcctaaaa
    22021 ctctgctcct gaacaatttt ataggagggg tagccagagg ctttggcact gctgtaggct
    22081 tcactattct tggagccgtt gtcatttata ttctaagaca gtctgtgctt ttgaatcttc
    22141 cttttatagg agggttcata gcagaaatag tcaagatagt tcaggatcat ctatgaataa
    22201 aaaaggaggc agaaaatttg gacaagaatg actttaagca ctttgaggaa ttgctgaagg
    22261 atgagaaaaa aaagagggaa aactcacttg aagatgagag gacagggctt ggaatttccg
    22321 tcaaagatct gaccggtgag ctttcttctt atgataatca tcccggagac atgggaaatg
    22381 agacctttga ggctgaaaag aatttttcct tcaggactag ggataaattt cttttaagtg
    22441 agattgacgc agctttgaac aggatagctg acggaacata cggttcatgt gagatttgtc
    22501 ataatgagat cggcaaagac aggctggaat taaggccata ctccagatta tgcatcaatt
    22561 gtgaaaaaga atacggttcg aatgtacaaa atgaggagaa gggcaggcct attgaggagc
    22621 aggtgcttta tccgcctttt ggcaggactt ttacggataa ttcggttgag gataaggtac
    22681 agtttgacgg ggaagatgcc tgggagtcag tgaacgaata taatgtaaga atgtcggata
    22741 atgaatttgg agaagacgaa tcggcagggt atgttgaaga tgtcgaacag gtaagtaatg
    22801 agcagtacag gaagcagttg gaatgaaaca gggttatatg ctataataac agttgtactt
    22861 atttgcattt aagggggaat atcattgtat cctgttctta ttgcagcagc gattatagta
    22921 tccgaccagg ttatcaagaa tatattggcc aatgctttgg cttcaggacc cataacggta
    22981 ataaagaact tcttttacct tgtttatgtg gagaactacg gaatagcctt cggcatgttt
    23041 aaaaacaagg tattgttttt tattgtcacc aattgtatta tatcggcagc ggtggcattc
    23101 atgatttaca agtaccgtaa taaaagcgtc cccgttacaa tttgcctgtc tcttattctg
    23161 ggcggagcga taggcaatgt aatagacaga ataaggttcg gatatgttat tgactacctt
    23221 tcattcacta tatttccgcc ggtttttaac cttgcggatt ccgcaatagt ggtgggcgca
    23281 attctgctta gcctgctgct tatattcggc aaggatataa aaatataggt acggggatac
    23341 ggggtacggg gtacgagggt tgggcaggac tttgaagttt ttggattatg aggtttcgag
    23401 attatgaggt gttgatgtat ggaggtaaaa gaatatatag cgggaccgga agcagaggga
    23461 gagaggcttg actgctttct gtcgggaaag gtggaaggct attccagaac ttatatgcaa
    23521 aagcttattg aagaagggca ctgcaaagtc aacggaaaaa atgcaaagcc caggtttaag
    23581 cttcgtgaag gtgaccgggt tgaagctgta atacccgatc cggtgccttt ggaagtggag
    23641 cctgagaaaa tagatctgga tataatttat gaagataatg atattgtagt aatcaacaag
    23701 cctcagggaa tggtggtgca tccttcccat ggcaattatt ccggcactgt tgtgaatgcc
    23761 cttatgggac attgcaaaaa cctgtccgac tataacagcc ttaccggaat aaacggggtt
    23821 atgaggccgg ggatagtgca caggatagat aaggacacat caggtataat agttgtggca
    23881 aaaaacaatg aagctcacct ttccatatcg ggacagctta aggagcatac aataacaaga
    23941 aagtatacag cactgcttga aggcagattg aaaaaagaat gcggaacagc ggataccctt
    24001 ataggcagaa acccaaagaa taggaaacag atggctgttg tgagggaaaa cgggaaaagg
    24061 gcagttactc actatagagt acttgagagc tttgagaacc ataccttgat tgaagcggta
    24121 ctggagacag gaagaaccca tcagataaga gtgcatatgg catatctggg acaccctgtt
    24181 gtgggggata cggtatacgg ctttaaaaaa cagcgctttg acacaaaagg acagctgctg
    24241 catgcaaggg tactcggatt cattcatccg gccaagaagg aatatgttga atttgaagcc
    24301 cccattccgg aatactttga gaaagtatta aggcttttga ggaatggaag attatagaca
    24361 aattatatat gctatggtaa aatctattaa aagcaacctt taaacttgtc cggtgaggca
    24421 tggaaggatt caaaaatata actgcaatct gcctcacggc agattttttg ctatgaaaca
    24481 gcatggagga atcattatga ggataaagac tgagataatg gatgagaaag cattggacag
    24541 ggcgcttacc agaatatctc acgaaataat agaaagaaac aagggagtgg aaggcctggt
    24601 tctgctcggt attaagacaa ggggagtctc ccttgccaaa aggctctgtg agaaaatccg
    24661 aaacattgaa ggcaatgtgc ttcctgtcgg aactcttgat attaccaggc acagggacga
    24721 tatcaaggat atgaaggata taccccggga aaatctgact gatatctcct ttgatatcaa
    24781 cggaatgaaa gtagtgcttg ttgatgatgt tttgtatacg ggaaggactg tacgagcggc
    24841 aatggatgcc atcgtggaaa ttggaaggcc cttgagcatt cagctggctg tacttgtgga
    24901 ccggggacac agggagcttc cgataagagc cgattatgtg gggaagaatg ttcctacttc
    24961 gagaagtgaa atgattattg taaatgtcgg agaagtggat ggcaaggatg cagttctttt
    25021 gggagaacaa gagtaatata cctttattgc tcttttttct tattattacg aaagagaggt
    25081 aattgaatga gattgcttat aaagcaatgc agcataatag atccgggaaa tacttatgaa
    25141 gggattaatg atatttatat tgaaaatggc ttgattaagc tgatagaacc ttcaatcggg
    25201 cttgctgccg ataaagtgat agatgcggag gggctttttc tgcttccggg attcatcgat
    25261 atgcatgcgc atctgaggga gcccggcttt gaatacaagg aaacagtata ttcggggaca
    25321 agagccgcag caaggggagg atacacctca atctgctgca tgcctaacac caggccggta
    25381 attgatgatg aagcatcttt atccatgctc caaaaaatca taaaaaagga tgcggcagtc
    25441 aaggtatacc ccatagctgc catatccaaa ggccagaaaa gcactgagct tgttgaaatg
    25501 aacaagctct cagaaatggg tgctgtcgct ttttccgatg acggaaggcc ggtggccaca
    25561 gcagcattta tgatgaaagc actgttgagc gccaaggaaa aaggttttat gataattgac
    25621 cactgcgaag aactgagcct ggctgagggc ggcgcgataa acaagggaag gaaatcggag
    25681 cagatgggca tacgggggat acatcccctt tctgaggaac ttaatgtgat gcgggatatt
    25741 atgattgccg gaaatacgta ttcgcatata cacatagccc atgtaagcac tgccggatca
    25801 gcagaaataa taagggcggc aaaaaaaaga gggatcaagg tcacatgcga agtgacgccc
    25861 caccatatcg gactgacgga ggatattatc acaccgggtt ttaccgactg caaggtgaat
    25921 ccgcctctta ggactgccgg agatgttgag gcgctgaagg aagcattgaa agacggcaca
    25981 attgacgtga tagccaccga ccatgcgcct caccataagg atgagaaaag cgaagatttc
    26041 tacagctctg cttttggaat atccggtctt gagactgcat tttccgtctg ttataccgaa
    26101 ctggtagagc ccgggatact tacattgaaa gaattaagtg aaagaatgag caaaaatccg
    26161 gcagaattat taaagctgga tacaggaata atcagaacag gcattgctgc tgatttggta
    26221 ctggttgata ccgcaaggga aattacggtt aatcggaatg atttcttgtc aaaaggtaaa
    26281 aacactccca ttcacgggaa gacatataag ggagatattg tatatacaat agttgacggc
    26341 aatgtagcct atgaaaataa aaatcggctt taggagggat ttgattgttt acagacatac
    26401 tcatagagaa agttgtaaaa tgcaggaatc atactgttgt gggcctggat ccaaggcttc
    26461 agtatctgcc tgattattta atcgatgaag ccgtggaagc agttgcggaa ggcagcagag
    26521 cttccgttat cgccgaagcg tttttcaggt ttaataaaga gataatagac aatgtgtatg
    26581 acattgttcc cgctgtgaag ccccagatag ccttttatga gcagtatggc accgcgggct
    26641 tcgaatgtta tttaaaaact tgcagatacg caaaatcaaa gggtttattg gtcataggcg
    26701 atgtgaaaag aggcgacata ggttctacgg cagaggctta ctctgatttt tatcttggga
    26761 ataataaagc ttcggcggaa accgattgca taaccataaa cccgtattta ggcaccgact
    26821 caatgattcc ttttgtaaat aactgcgaaa agaacggcaa agggattttt gtgctggtga
    26881 agacctccaa caagtcatcc aaggatatac aggacctgga gtcgggtggg aagaaggttt
    26941 atgagcatgt ggcggaactg gtagtaagac ttggcgaagg gctgacaggc aaatacggct
    27001 atagctcggt tggagcggtt gtaggggcca ccttcaggag cgaagggcaa aggctcagag
    27061 aactgatgaa aggggtatat ttcctggttc cgggctatgg aacccagggc ggtactgcag
    27121 aggacgctgc cgtatgcttt aatgatgatg ggcttggtgc tgttatcaac tcctcaagag
    27181 gcataattgc agcctataag agtgaagtat acagggaaag gttcagtgaa aaggagttcg
    27241 gacttgcggc aagggaagct gcaatagcaa tgaggcatga cataaacaat gagcttgccg
    27301 ggacaggtaa aatagcgtgg taggagttga agcatatatg cagtgcatat gtaaaattgt
    27361 tgagaacagg gaactatgca aagatgtatt cgaaatgaag ataaagtgcc cggaaatggt
    27421 acgggaagcc caaccggggc aattcctgca tttaaaggtt aataagtcaa ttgatccgct
    27481 tttaaggcgg cctataagca taagcaggat ttacagggat acaggtgtca tttcaattgt
    27541 ttaccagtgc gtaggcaaag ggacaaaaga aatggccgct cttaagaagg gtgaatacat
    27601 agatgtcctg gggcctttgg gcagcggctt tactttgtct gacgagggaa aatgtgccgt
    27661 agtaggcgga ggaatcggta tagcaccgtt attggagctt gcttgcagcc ttaaggattg
    27721 tgatgcctat ttgggcttcc ggtgcaatac ctataagctg gatgaattct cttcagcctg
    27781 taaagagctt tatattgcaa cagaagacgg aagtacggga agcaagggct atgttaccga
    27841 tattataaag gatataacga aatacaacgt tatatatgcc tgcggcccta aagggatgct
    27901 gaaaagagtc aaggagctgt gtgaggacac cggtgtgaaa tgctttgtat ccgtcgagga
    27961 aagaatgggc tgcggtatag gagcgtgctt ggtgtgtgcc tgcaggataa aagaagggga
    28021 tacatggcat tataaaaagg cctgcacaga cggcccggta tttgcggctg aggaggtgga
    28081 ttttgatgac tagaatgagc gtaaatatag ggggtataaa gctaaaaaac cctgtgctca
    28141 catcctcggg aactttcggc ttcggaaggg aatactccgg gttctacgat ctttcaaagc
    28201 ttggaggaat tgtagtaaaa ggcctgacac tggaacccag agaaggcaac aaacctccga
    28261 gaattgcgga gacaccggca ggtatgctga acagtgtcgg gctgcagaat cccggagtgg
    28321 agtacttcat tgagaaagag cttccgttcc ttcggggata tgatgtggca gtcatagcga
    28381 atattgcagg caataccgtt gaggaatact gcatgatggc ccgaaggctg ggaagcctgg
    28441 ttgacggaat agaactgaac atatcctgcc caaatgtgaa ggaaggcggg gctgcatttg
    28501 gaataagttc agaatcggtg tataatataa caagaagggt aaaggaacaa tgcaaggtac
    28561 ctcttatagt aaagctgtca ccgaatgtaa ccgacataaa ggaaatcgcc gagtcagcca
    28621 aagagggggg agcggatgca ttatccctca ttaatactct tctcggaatg gcaatagata
    28681 tcaattcaag aaggcctgtg ctggcaaaca atgtaggagg gctgtcaggc cctgcagtga
    28741 agcccgtagc tgtaggaatg gtatggcagg cagcccaagc agttgatata ccgataatag
    28801 ggatgggagg tataagctgc tgggaggatg caattgagtt catgcttgca ggtgcagacg
    28861 cagtgtcagt aggaacagca aacttcataa atccatatgc ccccttggaa attttggaag
    28921 gtattgaaaa atacctcata aaaaaccaat tcagcaatat aaatgaattg aaaggaaact
    28981 taaaaatata cggaggagat aacaagtgct tacaaaaaaa agagtaaatg gaattctaga
    29041 aaagacagga gtacttctga cgggacattt ccagctgacc tcaggcaagc acagcaataa
    29101 atatttacag tgcgcaaagg ttttccagca ccccgacatc agtgccgaga tatcaaagga
    29161 tctggctgag aaatacgacg gctatgatat agatatagta ataggccctg ctgttggagg
    29221 gataatactt gcatacgagg ttgcaaggca gctggaggta agggcacttt ttgcggagag
    29281 agagaacggt atcatgactt tgagaagagg ctttgaaata cagcgtggct caaaggtgct
    29341 ggtggttgag gatgtaatca ctacgggagg ctcagttaaa gaagtgattg atgttgtgcg
    29401 ctctgcaggg ggagaagtgg tcggagttgc cgcggtagtg gacagaagcg gcggcaaggc
    29461 cttattcgat gtacctttta aaagtgctat aaaaatcgat atagaggtct tcgagccggg
    29521 agattgcccc atgtgcaagt cgggttccaa agcagtaaaa ccgggcagca ggaatatata
    29581 atatatatta tatgatttga aatgttaaat taataacaca taatgtataa aaagccttaa
    29641 atttcaatag ataaaagtat caataatgct aatttatttc atcttattaa aaataataca
    29701 taatgattgt tgaatttttt gaaaaatcaa tgtattatat tattaatata acataaagga
    29761 ggtttaatta atgactgaaa aaagagaaaa ttggggatca cgatttggat ttcttatggc
    29821 agcagcaggc tcagctgtag gacttggtaa catttggaga ttcccatacc tgaccggtat
    29881 gaacggcggt gcggctttca ttattattta tttaggttgt gtnnnnnnnn nnnnnnnnnn
    29941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30001 nnnnnnnnnn nnnnnnnnnn nnttacttct gcaatcatgg cggtaatgtg tatgctttct
    30061 tcattgtcaa tgggagttat gtcaggattt acgatttttg gagtcggctt ctttgacttc
    30121 tttgatatcc ttactgacaa aatattcctg gctgtcggag gaggcctcct tgcaatatgt
    30181 gtcggattct ttatgaagaa ggaagacctg aaggatgagc ttacaaatgg cggaacaata
    30241 gaatttggat tattcgaatt gtggtacaac gtaacaaaat atgttatacc tgttattatt
    30301 gcagttattg cattttcagg tattatggca atacaacaaa agggcttgat gatattcggg
    30361 cttgtaatta ttgcagtact ggcagcattc tccaaaaagc tttaaatctt aatatttatg
    30421 taaaaataga gctgttgcat atgcagcagc tctatttttt catactattc actttttctt
    30481 catctggtgt aatatacatc gtaaagtagt tgtcatagac taccattccg ggcttggcgc
    30541 cgccgggttt cttaacattc tttcttctgg tataatccac aggtaccttg gaggacatcc
    30601 tgcctttgct gaaaaatgcg gctaaattcg ctgcttcctc aagagtgttg tcgtccacat
    30661 ctcttccttc agtctttatt aggacatgag aaccgggaat atcttttgta tgaagccata
    30721 tatcttggct tgaagcaaat ttgagtgtta gatagtcgtt ctgcatatta ttctttccga
    30781 caaatatttc aaaacctgaa gaggaaacaa aatgcatagg tgtcgaggtt tttttcgtct
    30841 gcttgccttt tgctgccctt ctttttaaat agccttgttc cgccagttct gctctgattt
    30901 cttccaattc cagctcttct gtgcatttgc ttaggttatc aagctgggtt tccagataca
    30961 ttatctcctg tttgttttct tcaagctgtt ccgttatctt gattattgca tttttagact
    31021 tgttgtagtg cttgtaatat ttttgggcgt tttctgaggg ggaaagacgg atatccatag
    31081 gaatatcaac atattcgccc tctgaggagt agtaattctg aagccttgcc ttttcttcgc
    31141 ctctttgaag ataatatatg tttgacatga tgagatcgcc atatatcttg aatttttctg
    31201 aatcagcggc ttcctgaaga tctccgttca gcctttcaag cttcttgaag cagcgttcca
    31261 gcctgtttga aataattcta tatatatctc ccgatttttg cttaattctg tccttttgat
    31321 ccttctcatg atagaacctt tcaactgcct cactcattcc ttggaattcc tgcttctcta
    31381 agtctctgta tatttcaagg tcaaagcaga agaaatccaa ggccttgccg cccttgtaca
    31441 ctatattcgg tttaaaggcc gc
//
LOCUS       DAOOAB010000030        31364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1880_length_31364_cov_16.113354, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000030 DAOOAB010000000
VERSION     DAOOAB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 31364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31364
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_31364_cov_16.113354"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..993
                     /locus_tag="PKU88_07805"
     CDS             382..993
                     /locus_tag="PKU88_07805"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPX69217.1"
                     /translation="MNKLERSFYLRDPLIVAEDLLGKHLVHETRDFRVVGKIVETEAY
                     AGPEDRACHAYGLRRTKRNEIMYHEGGFSYVYFIYGMYYCFNVVTEQKDNPSAVLVRA
                     VEPIDGIGHMARLRYGMDLLSLSASQRSVMCNGPGKLCMSMGIGKAENGLDLCGSTLY
                     ICDAHNEPFSVKSSPRINIDYAGEYKDKPWRFYIEGNKHVSKG"
     gene            complement(1014..1676)
                     /locus_tag="PKU88_07810"
     CDS             complement(1014..1676)
                     /locus_tag="PKU88_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPX69218.1"
                     /translation="MPSYYELFVRLALACFLGGVIGYEREKTRHPAGFRTHILVCVGS
                     ALAMLCNIYLFEEYKGIANIDPGRFGAQVISGIGFLGAGTILKEGATVKGLTTAASLW
                     SVACIGISVGLGFYLGSLLTTAFVLVALVVFSRFEVLISGGKKNTTLKIRSEDKPGQL
                     GKIGTHLGSFNLKICDIAIEVEDEASVMVKIKVNRAKENISPEVLDSLSKIEGILKVE
                     VC"
     gene            1927..2763
                     /locus_tag="PKU88_07815"
     CDS             1927..2763
                     /locus_tag="PKU88_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPX69219.1"
                     /translation="MISEAEEKYMVINGKVMPVSGAGDILAGSSRTIYEVIKVVSGIP
                     LFLEKHMERLEASARLINYSVTNIMNSIRESITELIKANNSPEKNIKIIVYNMGNPVP
                     DFMAYFIRSSYPTPEQYETGVHGILLREERNNPNAKVVNSGYKERVAAALAEANAYEA
                     LLVNREGEITEGSRSNIFFVRNGKVLTAPKGNVLIGITREYVFDLCKNLGIEIIEEPI
                     SVSMLGEMDGAFMTGTSPKILPISTIDDMSFSSPKNPVIKALISSYNDIVEKYIKEKT
                     IG"
     gene            complement(2766..4112)
                     /locus_tag="PKU88_07820"
     CDS             complement(2766..4112)
                     /locus_tag="PKU88_07820"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HPX69220.1"
                     /translation="MDKIILGKTIGQWIEEFPIIDNLMSCKPVFWTNKNLDNADKVLP
                     ELSLTLEDMVDAEKRLQRFAPLISVLFPETADKGGLIESELVCIGKMKNSIQKVYRQD
                     FQGELMLKCDNYLPVAGSIKARGGIYEVLKHAEDLAVKHGLIRIDDDYSRLGSPEFKE
                     FFGKFSLAVGSTGNLGLSIGIMGSALGFNVTVHMSADAKEWKKSLLRGRGVNVIEYSS
                     DYSKAVEEGRRQSQQDPNSYFVDDEHSKSLFLGYSAAAFRLKKQLDALGTKVDESNPL
                     FVYVPCGVGGAPGGICFGLKHVFGDNAHVFFAEPTHSPCMLLGLATGENEKLSVQDFG
                     IDNITDADGLAVGRPSGFVGNMVGRLLSGIYTVEDNELYKLLAMMKDEENIKIEPSAT
                     PGLLGPLQAEAYINANLPEVSMKNATHIAWATGGLFVPEAMMQEFYERGKKLLGEL"
     gene            4350..4793
                     /locus_tag="PKU88_07825"
     CDS             4350..4793
                     /locus_tag="PKU88_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HPX69221.1"
                     /translation="MPLATREIFARMIKCEAGGEGDNGMKAVATVIMNRVHVPNGEYQ
                     RIGQGDLRRILFQPGQFDCASDTLGGAYNAQNIYNMRPDQIHYDIADWALSGNKMLTV
                     AYCLWYFNPFTQTCIQTFPRNGSGTFANRIGEHCFYNPTRLYYTT"
     gene            4869..5354
                     /locus_tag="PKU88_07830"
     CDS             4869..5354
                     /locus_tag="PKU88_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69222.1"
                     /translation="MYSNSSYSFNQTPASSGNQSQFSQAPEMGTTFGYPMAPQTFPIQ
                     QQMGAQQMGGQQMGQQMPQMPQMPPPTMQMPVTGAAPVDFDMLPTTPVLDIEYTQGYL
                     KTQIGKRVKVEFLIGTNMLIDREGTLVDVGISYIIINEVETDDLLLCDIYSIKFVRFY
                     F"
     gene            complement(5480..6655)
                     /locus_tag="PKU88_07835"
     CDS             complement(5480..6655)
                     /locus_tag="PKU88_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPX69223.1"
                     /translation="MKTDQDRIKSLTEYVQGLLDKENGRELYFKYRTYIERVTPQEVF
                     EIFHLILQKGIKPKEILTVLDKAINVFYKSLSNYTWKWPDKDSFMDYLMRENRALEEK
                     LEGIKEIIKDMSSEAGKKELILRMHELRKFNDHYLKKENILFPYLEKKMKKFDGLAIM
                     WALHDEARAQLKKVIECLEAEGCIEGEFNSELGKLFFDMYGLVQKEELILFPAASEII
                     TDDEWLEMQRQSMEYEFPFIERPEPADNEYIPGIKEGCRFETETGALDFNEILAIFDA
                     LPVDLTFVDENNKVKFFTKPKDRIFPRSPAVIGRDVEKCHPPESVHVVNEILDAFKEG
                     RKDNAVFWIKVKGRMILIQYFAVRDSVGRYLGTLEASQDITDIIKLEGERRLLQFNK"
     gene            complement(6664..7476)
                     /locus_tag="PKU88_07840"
     CDS             complement(6664..7476)
                     /locus_tag="PKU88_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035109.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPX69224.1"
                     /translation="MSSIGIYYFSGTGNSLYVAKELQKRIEGAKLIPVISRLDREVVE
                     TDAETIGFVFPVYFSAIPVPVKNFIKKLDLKSAKYIFAAATRIGTSHSAFMAIDKILK
                     SKGQRLDSYFSLNMPGNDPKFNYKVPTEEEIARLEAVVQKRLDSIQSIIKNKEISREK
                     DVDCTIHIPFVGILSSLAALIDISKDNLYCDSKCTGCGICEKVCLAHKIKIVDGKPEW
                     QDNAKCYHCAACLNYCPVQSAQIKSQTEKNGRYSHPYAAVEDIAMQKNFERR"
     gene            complement(7492..7893)
                     /locus_tag="PKU88_07845"
     CDS             complement(7492..7893)
                     /locus_tag="PKU88_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPX69225.1"
                     /translation="MKSRRRVVLAVVIALAAIVTAVFGFKSYTEASLVKLAETSIPDV
                     DLSKIADGVYKGSYKVFPVEAEVRVTIENHKIVAIELVKHFNGQGAAAEVIPDRVTEA
                     QSLGVDIVSGATYSSKVILKAIENALQSANR"
     gene            complement(7890..8390)
                     /locus_tag="PKU88_07850"
     CDS             complement(7890..8390)
                     /locus_tag="PKU88_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015594182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPX69226.1"
                     /translation="MKIGIIVHSKTGNTYSVAEKLMKTLAAKGHIASIEQVEACDNGQ
                     MDAIKVQLKSKPDINPYDALIFGAPVHGFSPSVVMEAYLTQLMAIPDKKAACYVTEFF
                     PLPQMGGNQAIERMKEICSSKGFDVIGTGVVNWSNIHRKGMIDNLAEKFSGLFMSLSE
                     CKEGNK"
     gene            complement(8387..9001)
                     /locus_tag="PKU88_07855"
     CDS             complement(8387..9001)
                     /locus_tag="PKU88_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX69227.1"
                     /translation="MKEKTAEDKKILKKQRIRTYFLEAAREIILNDGVESVTVRKVAD
                     MAGYSYATIYNYFSDLNQLLWEVKKLMINDIVEALYKKMAGMTKDVDGIRKCFKMYVE
                     YFLVNPNVFRFFYFHHLSKPAGETGENEEEPDFDAMWEETFKEFVISGKIRAEDIEVL
                     ARTMIYAIHGLITLYFSEDVDYKGEDVYSELDKVIEYILGRGQI"
     gene            complement(9198..9356)
                     /locus_tag="PKU88_07860"
     CDS             complement(9198..9356)
                     /locus_tag="PKU88_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69228.1"
                     /translation="MKYSPKPTFEFYKAYRKQINQIKKEVDASLQVDNGAFCGFLMMS
                     LESSEDTE"
     gene            9500..10024
                     /locus_tag="PKU88_07865"
     CDS             9500..10024
                     /locus_tag="PKU88_07865"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HPX69229.1"
                     /translation="MDLKRWQIIGAVFALIMGALLHFTYEWSGENPVVGIFSAVNEST
                     WEHLKLLFVPVALFAALEYIGYGKQIPNFVPVKVLSILLGMITIIIAYYTYTHIVGQN
                     YLWVDIGIFILGVLAAYWFSYSLLQTEFLASECAVCLGWAGLLLLAFSFAFFTFCPPH
                     VELFLDPVTGTYGR"
     gene            complement(10084..10665)
                     /locus_tag="PKU88_07870"
     CDS             complement(10084..10665)
                     /locus_tag="PKU88_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX69230.1"
                     /translation="MDCSKVGKLIFGLRKEKGMTQKQLADAMNISDKTISKWERGLGC
                     PDVSLLRELSEVLNADIEKILLGDLEPNNADGGNMKKIKFYMCPNCGNTLTSTGESDV
                     SCCGRKLVPLIPKPAEGTHKLTVEEIEEDFYITFAHEMSKTHYINFVAYVAYDRVLFV
                     KLYPEQGSEVRFPKMYRGKIYLCCNQHGLWVND"
     gene            complement(10670..10915)
                     /locus_tag="PKU88_07875"
     CDS             complement(10670..10915)
                     /locus_tag="PKU88_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69231.1"
                     /translation="MKKGLAVSPGIGIGKAYIISEPDINIDKGRIDAEQVKEELDRGQ
                     FIYSYKWRIHRFLILHRNSTERLLNYSPSAIIINAWR"
     gene            complement(11017..12168)
                     /gene="nagA"
                     /locus_tag="PKU88_07880"
     CDS             complement(11017..12168)
                     /gene="nagA"
                     /locus_tag="PKU88_07880"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017531532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPX69232.1"
                     /translation="MKAIVNARIIKDNSIFDGYTVLFEEKILRLMEGNALKGLEPEEV
                     FDAAGRYLSPGFIDVHIHGCIGNDTMDDNESSLRNISKAIAATGVTAILPTTMTMGFP
                     MIEKSINRIRTLMDDAPGASILGCHLEGPFISEEYKGAQDGKYIRRPDFKTIEGLSDV
                     IRIVTIAPEEEGAEEFIESCVSQGIVVAIGHTNASYEQAMRAIQKGASHITHIFNAMT
                     PLNHREPGAVGAAMDSSVVCELIADNVHVNPAVQRILLKVKGVEGIILVTDAMKACLL
                     EDGEYELGGQRVVVKNEVARLPAGNLAGSVLTLDKALRNFTANTGIGLIDAIKTVTEN
                     PARVLKVNDRKGSIDIGKDADFVLFDDSFNIHATFVKGKKVYERVVKWE"
     gene            complement(12169..12456)
                     /locus_tag="PKU88_07885"
     CDS             complement(12169..12456)
                     /locus_tag="PKU88_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69233.1"
                     /translation="MRINANTEKIDKLSTIEMVTLFNNEDKKVAVIDASECPPTFGID
                     DNVIIGVISGRIEAVSGWKEEEALKAFEKAGGNARAAILSLVNDSSKIEGD"
     gene            complement(12511..13086)
                     /locus_tag="PKU88_07890"
                     /pseudo
     CDS             complement(12511..13086)
                     /locus_tag="PKU88_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(13255..13491)
                     /locus_tag="PKU88_07895"
     CDS             complement(13255..13491)
                     /locus_tag="PKU88_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69234.1"
                     /translation="MNNGWALTSPTGESFIWGKDFSSEGTPDVMLEFDSQPFIDAGAD
                     PNKVEGWVFAKVPVMDEQGKKIEVEKLLKPFDLK"
     gene            complement(13658..13978)
                     /locus_tag="PKU88_07900"
     CDS             complement(13658..13978)
                     /locus_tag="PKU88_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPX69235.1"
                     /translation="MSSKGGIPLDCNCDHNVTVRTEKEKKDIISRLNRIEGQVRGIKG
                     MIEKDVYCDDVLNQISSVNSAMNSVGKLILKNHMKSCLIKRIQAGELDVVDELLYTIE
                     KMLK"
     gene            complement(13979..14194)
                     /locus_tag="PKU88_07905"
     CDS             complement(13979..14194)
                     /locus_tag="PKU88_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPX69236.1"
                     /translation="MKKQINIEGMSCGHCVKHVEGALGEVPGVIKVDVSLEGKYAIVE
                     LEKEVPDSVLKNAVDEAGYDAGAVKVL"
     gene            complement(14293..16755)
                     /locus_tag="PKU88_07910"
     CDS             complement(14293..16755)
                     /locus_tag="PKU88_07910"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPX69237.1"
                     /translation="MMRSDIFRINGMTCAACAKAVERAVKKLDGVSDATVNLATEKLS
                     LTYDEAKLKPSDIAEAVSKAGYEALQEKNTKEVSIPIEGMTCAACAKSIERAVGKLSG
                     VEAVSVNFATEKANVKYDPAELRLSEIKQAIVKAGYKALEIESKDKVDEDKKRKDREI
                     RILWTKFIVSAVFAAPLLYIAMGHMLGLPLPKALHPMDFPLRFALVQILLTVPVVTAG
                     YRFYTVGFKAILHRSPNMDSLIAMGTSAAVLYSLYSTYQISIGHFMYAEDLYFETAGV
                     IITLILLGKSLEAVSKGRTSEAIKKLMGLAPKTAIVMQAGEEIEIPIEEVEVGDIIAV
                     KPGSKIPVDGEVVEGLTAIDESMLTGESIPIEKKPGDKVFAASINKTGSIRFKATKVG
                     SDTALAQIIKLVEDAQGSKAPIAQMADIVSGYFVPIVFVIATLSALAWYFIGGETPVF
                     ALTVFIAVLVIACPCALGLATPTAIMVGTGKGAEYGVLIKGGEALETTHKIKTIVFDK
                     TGTITEGRPEVTDIVTTEIIKKDKLLQIAASAEKGSEHPLGEAIVRGAEKENLEDLKV
                     DKFNAIPGQGIEVEIEGKNILLGNRKLMTERNISLSGLETESDRLASEGKTPMYAAIG
                     NQLAGIIAVADIVKESSAKAINKLHEMGIEVVMITGDNRKTAEAIAKQVGIDWVLAEV
                     LPQDKANEVKKLQSEGKKVAMVGDGINDAPALAQADIGIAIGSGTDVAMESADIVLMR
                     SDLMDVPTAIQLSKSTIRNIKQNLFWAFGYNVAGIPVAAGLLHVFGGPLLNPILAAAA
                     MSLSSVSVLTNALRLKGFKPYK"
     gene            16924..18714
                     /locus_tag="PKU88_07915"
     CDS             16924..18714
                     /locus_tag="PKU88_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPX69238.1"
                     /translation="MSIKKEKIKIFEMTCTSCENRVEKAVRQLEGVIYAKASYSGQYM
                     EIEYDDDLCGMNNIKDAVKNAGYSTEGSKDCKFMGILIIAVGVVLLGLKTGGFDMEAK
                     LTNASYAVLFIVGVLTSLHCVGMCGGIMLSQSLSKESKNRFEAIQPALLYNIGRVISY
                     TILGGLIGALGSVFTLSIAAKAAVQIFAGAFMIIMGLNMAGFSAFRKFQIRLPQFACK
                     AKSKSGSPLIVGLLNGLMPCGPLQTMQLFALGTGSAAKGALAMFVFSIGTVPLMLTFG
                     ALSGLLSKGYTKKILKFSGVLIIVLGLIMGNRGLALAGININPITAFASNSAGSSNPY
                     VAKATLQDGIQIINMTADNNGYAPNAFYVQKGIPVRWIIDGKELNSCNNAIVARGLNL
                     ERKIKKGENIIEFTPGDKDINFSCWMGMISGVIKVVDNLDSVDTSKADPSLPPPSTGP
                     SCCAVPIEEDEPSQPSIYGDDISKVPTEKLVNKALVDGKNQSVIFKGIGYELQPLIAV
                     AGSGGKIRLTLDLNDFDNAEGEYLIIDAVTGKDVTAFTAEKGINEIEFSPKNVDGYAI
                     IKGNSILGIIEVVDNLEAADIKEIREKYLP"
     gene            complement(18820..19593)
                     /locus_tag="PKU88_07920"
     CDS             complement(18820..19593)
                     /locus_tag="PKU88_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPX69239.1"
                     /translation="MKQLRLSAAFIAVLLLIVTSFGVIIVQGAEPEIPNPTREFYVND
                     FAGILDSGTERDILALGENTYKATDGGQVVFVSIDTLNGNTIEEYANTLFNKWKIGTE
                     DKGILFILSMKERESRIEVGYGYEGVLTDLESSKLLVKFAGLYEEKGMDEAVKTIYSD
                     IANIVSGNEDMVRYPEIGEERQYTSDDFSFFRENPIITVILVIIILILIVLDFIFTGG
                     RVTFFIIRMAASSGRRGGGGGRNSGGGGRSGGGGASGRF"
     gene            complement(19685..20248)
                     /locus_tag="PKU88_07925"
     CDS             complement(19685..20248)
                     /locus_tag="PKU88_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPX69240.1"
                     /translation="MKNKSWIIIIGIVVLLAVYVVGVQRSLVNLSEEVNASWSDIEVQ
                     YQRRADLIPNIVETVKAQAIKEQEIFTQIAEARSKLSGAANIQEKIDANAEVSSVLGR
                     LLAIAENYPELKQNQGFQDLRVQLEGTENRIAVARKYYNDNVKEYNKKIKVFPTNIAA
                     GILGYSDEFPYIEAEAGAEQAPDVNFD"
     gene            complement(20453..21844)
                     /locus_tag="PKU88_07930"
     CDS             complement(20453..21844)
                     /locus_tag="PKU88_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX69241.1"
                     /translation="MKVSLRTKLTLSHVATALICVALISLFSNILLERHFQDYIRSNQ
                     EKKNKELVSLFSSNYQSNGQWNAESVQDIGIYALEQGMIVEVRDQSGNIIWNAMSYNN
                     GWCNQMLDHMANNMYSRYPNWEGKLVQVNYPVFYKGEKVGSIDIGYYGPFYFNDNDMA
                     FISTLNRIFISVGVFSLLLAFLFGSIMSRRITMPITRVINTARMIAKGYFGDRSFERT
                     STKEISQLTDTINDLAETLETQEKLRKRLTGDVAHELRTPVATLQSHLEAMLDGIWEP
                     SSDRLKSCHEETMRINRMIGDLEKLARYESDNMILNEEEFSLCELIKIMVQNFEKEFI
                     QRNIKIQIQCENIMLFADRDKISQVLVNLISNALKYTNSNGQVTITASDSGDNTTIAV
                     RDTGIGISEEDLPYIFERFYRSDKSRNRTTGGSGIGLTIAKAIVEAHKGSISASGKLN
                     EGTVFTVALPKKR"
     gene            complement(21862..21990)
                     /locus_tag="PKU88_07935"
     CDS             complement(21862..21990)
                     /locus_tag="PKU88_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004837889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LDCC motif putative metal-binding protein"
                     /protein_id="HPX69242.1"
                     /translation="MKNLKKIIDDFLKRLAAQNKEQFGSEKLDCCNLDRPKNDSRE"
     gene            complement(22154..22384)
                     /locus_tag="PKU88_07940"
     CDS             complement(22154..22384)
                     /locus_tag="PKU88_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPX69243.1"
                     /translation="MVIKVLGSGCMNCRKLEENTKAAVKELGIDAKVEKVENYKDILA
                     YGVMKTPALVVNEEVKVMGRVADTEEIKKILR"
     gene            complement(22411..23427)
                     /locus_tag="PKU88_07945"
                     /pseudo
     CDS             complement(22411..23427)
                     /locus_tag="PKU88_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(23594..24658)
                     /gene="arsB"
                     /locus_tag="PKU88_07950"
     CDS             complement(23594..24658)
                     /gene="arsB"
                     /locus_tag="PKU88_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPX69244.1"
                     /translation="MNNKQKSIERDNEALGFFAKYLTVWVAICIAAGVILGQVLPIVP
                     ETLSKFEYANVSIPVAILIWLMIYPMMLKIDFSSIVNATKKPKGLIVTCGTNWLIKPF
                     TMYAIAALFFHVVFKALIPAELAKEYLAGAVLLGAAPCTAMVFVWSHLTKGDPAYTLV
                     QVAVNDLILLAAFTPIVAFLLGVSDVAVPYDTLFLSVILFVVIPLAGGYFSRKYIIKN
                     KGLDYFNNVFLKMFDNTTVIGLLLTLIIIFSFQGEIILNNPLHIALIAVPLTIQTFLI
                     FFLAYGWAKAWKLKHDIAAPAGMIGASNFFELAVAVAISLFGLESGAALATVVGVLVE
                     VPVMLALVKIANSTRHWFAE"
     gene            complement(24659..24994)
                     /locus_tag="PKU88_07955"
     CDS             complement(24659..24994)
                     /locus_tag="PKU88_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPX69245.1"
                     /translation="MNLDYNENAKIIKALSDPSRLKIIDILSCGEMCACDILKHFDFT
                     QPTLSHHMKVLMECGIVECRKEGLWNYYSLNGTNCNKLILFLMSIITNTDDCICKEKP
                     KCGCECEGK"
     gene            25215..25541
                     /locus_tag="PKU88_07960"
     CDS             25215..25541
                     /locus_tag="PKU88_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPX69246.1"
                     /translation="MNISRICNALGNEVRYQIVKSLKNRSIATCCNRIEYYENGISVG
                     DVVNATGLAQSTVSQHIKVLLDAGIIYKEKRGQWTCFFLNGEIINNFIKGLSHDLIQT
                     ECDCKK"
     gene            25603..26256
                     /locus_tag="PKU88_07965"
     CDS             25603..26256
                     /locus_tag="PKU88_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPX69247.1"
                     /translation="MINLLNETISYVAGTLLHNAPVLVFGILTAAAVKVYADQEKLRK
                     VLIKKAGSFAEKYKLKELFKVFYEVGIKQVILYFAIFAAIGFLINRFVPAEVITKYLG
                     TDNKFAVPLMALVGLPLFVSGSSSIPILNTLIAGGASQGALLAFMITGPGTSAGVIAG
                     LAVIMKRKAIGLYIAYFLAFGILLGYLYDFLLMLVISPFPYHPQTAHYTGMRILRRC"
     gene            complement(26186..27934)
                     /locus_tag="PKU88_07970"
     CDS             complement(26186..27934)
                     /locus_tag="PKU88_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69248.1"
                     /translation="MSKIRRFISYYKPYKGLFFLDMFCSLTVAGIDLLFPVLVRYLLD
                     HGINVEIGVAFDIILKIGALMLGMHILKYFCQYFITAWGHIMGARIEYDMREEIFSHL
                     QKLSFSFYDNNKTGHIMSRIVNDLFDISELAHHGPEEVFISIIKILGAFLILLRINMK
                     LTLVTFAFVPLMMGFAYFYNNKMRSVFRRNRQKVAEINAQLEDSIAGIRVVQSFSNER
                     EEQEKFTRGNRGFLDTKQESYFYMGRFFSGIEFFDGIIYIFAVIAGAYFIKLGTAATS
                     DLVAYLLYINTFLVPIRSLIHFTEQFQKGMTGFERFLEILDTEPDIKDGEDSISLEKV
                     EGRVEFTDVSFKYGEGGQVLKDINLNLEPGETVALVGPSGGGKSTLCSLIPRFYEASE
                     GIIKIDGVDIKNIKLKSLRKNIGIVQQDVYLFAGTVTENIKYGKPGASDEEVIAAAKK
                     ANAHDFIMTLEKGYDTYVGERGVKLSGGQKQRISIARAFLKNPPILILDEATSSLDNQ
                     SERIVQQSLEELSSSRTTFVIAHRLSTIKRAKTILVLTDNGIEEKGNHEELISSGGVY
                     ASLYNAQFEDDMEMEI"
     gene            complement(28045..28926)
                     /locus_tag="PKU88_07975"
     CDS             complement(28045..28926)
                     /locus_tag="PKU88_07975"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPX69249.1"
                     /translation="MKIYYFSGTGNSFAVAKDIAKKADAELISIPGAMYMDKIQVDAE
                     SIGIIFPSYLAPLSGVPLIVERFVMKIANIGSIYIFAVCTCGGYECVNALPSLERLRH
                     IIGFCGGKLSAEYSVRLPMNNLDYDHIPIPIIRDQEIIINKSKAKIDDVCYRIINKRG
                     TKYKIVKKLFGLLMTPLYKLIRQSVINDLKEKAKEPMDTGLKYFELIPLTDKSIIIDE
                     KCNGCGTCEKVCPVNNIKIVDKKPEFQHKCEMCFACDEWCSYNAIHHWSRTVGVKYHH
                     PEVEISDMLCKKVLDGN"
     gene            complement(29121..30482)
                     /locus_tag="PKU88_07980"
     CDS             complement(29121..30482)
                     /locus_tag="PKU88_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HPX69250.1"
                     /translation="MANIDKQHVIESNKLSGEQYFTSILQGAYANGLLCDSDMENIQL
                     QCIQLLANICERYSGGESSSIRVEAAENIMKSNLYTIGLYLKSLPDVDFAVNEIKKAS
                     VSEMYQKGRKLINVRLHSAKHIYNLVRKNKLFTPNYSYNSTINEGIGSFFKAYDPDYE
                     AHETPASIDYQLCNPVTNMIGIEFILKYLENLFLENEFCRGFAPEDIHHMLYGYDKGY
                     KDLLINIFEQVLTAALGCSLANRNVVELDISAEEIRHLHYELSEYDDCSLSLKLRKAA
                     GKMLEELNADSLPLKRYIERSMPKIITNILHAVRMNTLNKTFISKALPDLKPRIRFLS
                     GTKMHDEDYRKLIDELLMCRHSPDKLALIKEKVKSFGDLEDLLFDAQLSEEETDSVFD
                     ILEDVEIAALIRRHPFRTDIQAVDLSEGEMALRQYLKNYIDRLPKGKQEEIFEMADRL
                     IDE"
     gene            complement(30469..30960)
                     /locus_tag="PKU88_07985"
     CDS             complement(30469..30960)
                     /locus_tag="PKU88_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HPX69251.1"
                     /translation="MSFEIIGISSSLIEKQAITEIERCNEFTAEFGLALSPSDAAELV
                     EARNIALKSNGRIEFGGGAIHKIIREFCDSPYISMHNYAETIHELTEMFYYYKNETLD
                     LMSDDDLIKFMKNSFDGKCQGSLELLSGRELANMARNLRYGYPPDYSEDPMPDEEDED
                     GEY"
BASE COUNT         8745 a         7432 c         5687 g         9500 t
ORIGIN      
        1 attatatagt aatattttca tattgtaaat tgctcatgct catactacta ttattgtata
       61 ctatatgaag aatgcgacgc attcttcggg tacgaggtac gaggcgcgag gttcgaggtt
      121 tagggcaggg cttcggagtt atgataacac tgaagacacg aaaaaccgct aaatgcacgg
      181 cgggataaat attacgttac ataaataatt atcgataata atataagtat cgaaattttc
      241 ttatgcaact atataatata taatagttac cgtgcaatcg cgcagcgatt tcgtgaaaca
      301 gccgtgtctc ccgtgttacc cgttacctat ggcgcattat tcttcaaaaa cgaaacataa
      361 aaaaatatag acggaggtac tatgaataag cttgaaagga gcttctatct tagagatcca
      421 ttgatagtag cagaggatct gctggggaag catctggtgc atgaaacccg ggattttaga
      481 gttgtcggaa agattgtaga aactgaagca tacgcaggcc ctgaagacag agcctgccat
      541 gcctatggcc tgagaagaac caaacggaat gagataatgt atcatgaagg aggcttctcc
      601 tatgtatatt tcatttacgg aatgtactac tgcttcaatg tggtcacaga acagaaggat
      661 aatccctcag cagtactggt gagggcagtt gagccgattg acggaatcgg gcatatggcc
      721 agattaagat acggcatgga tctgttatca ctgtctgcaa gtcagagatc cgtaatgtgc
      781 aacgggccgg gaaagctctg tatgagcatg ggtataggaa aggctgaaaa cggacttgac
      841 ttgtgcggca gcacacttta tatatgcgat gcacataatg aacctttttc tgtaaagtcc
      901 tccccgagaa tcaatataga ctatgcaggg gaatataagg ataagccttg gagattctac
      961 atcgaaggaa acaaacatgt atcaaaggga tgatttaata aatcccttca atatcagcaa
     1021 acttcaacct tcaatattcc ctcaattttg gacagggaat ctaaaacctc agggctaata
     1081 ttctctttgg ccctatttac ctttatcttt accatgaccg aagcttcatc ctccacttct
     1141 atggctatgt cacatatttt cagattaaat gaacccaaat gggtgccaat tttccccaac
     1201 tgccccggtt tatcctctga tcttattttc agggttgtat ttttcttgcc cccggatata
     1261 agtacttcaa atcttgaaaa gaccacaaga gcaacgagta caaaagcggt ggtaagaagc
     1321 gagcctaaat aaaaacccag tcctacggat attcctatac aagcaaccga ccaaaggctg
     1381 gctgcagttg tcagcccctt gacagtcgca ccttctttca atattgtgcc tgcccccagg
     1441 aatcctatgc cgcttattac ctgtgcgccg aaacgtcccg gatcaatatt ggcaatacct
     1501 ttatactcct caaataaata aatattgcac aacatggcca gtgcagatcc cacgcatacc
     1561 aatatatgcg tcctgaagcc cgcaggatgc ctggtctttt ccctttcata gcctatgacc
     1621 ccgccgagaa agcaggcaag agcaagccgg acaaacaatt cataataact tggcatgaaa
     1681 ttccacctcc ttttatgata tatatcggct tatcgtgcca atattcctaa cccgtattta
     1741 atatatattc cgtgaaaaag ttttatcacc ccaaaagcag cagtactttt tattaaagta
     1801 atgtgtttcc cggcgcatta atttcggaaa atgtttgaca ctaagcatat acctcttgaa
     1861 atataataca cataatgcta gatttctatt atagaacaat taattacaga gacaggagac
     1921 agattgatga tcagtgaagc agaagaaaaa tatatggtta taaacggcaa agtaatgcct
     1981 gtttcagggg caggggatat tttggcaggc tcttcccgaa caatctatga ggtaataaaa
     2041 gtagtgtcag gaattcctct tttcctggaa aaacatatgg aaaggctgga ggcttcagcc
     2101 aggcttatca actattcggt aaccaatatc atgaatagta tacgggaaag tataacggag
     2161 cttatcaaag ccaataacag tcctgaaaaa aatataaaaa taattgttta taatatggga
     2221 aaccctgtgc cggactttat ggcgtatttc atccgcagca gctacccgac ccctgaacaa
     2281 tacgaaacag gagtacatgg aattcttctc agggaagaaa ggaacaatcc caatgccaag
     2341 gtggtaaatt caggttacaa ggaaagggtt gcagctgctt tagctgaagc aaacgcctat
     2401 gaagcactgc tggtgaacag ggaaggtgag attaccgaag gaagccgctc caatatattt
     2461 tttgtcagga acggtaaggt attgaccgcc ccaaaaggga atgtactgat aggtataacc
     2521 agggaatacg tatttgatct gtgcaagaac ctgggaatag aaataatcga agaacccata
     2581 tcagtttcca tgcttgggga aatggacggt gcttttatga ccggcacctc accaaaaata
     2641 cttcccataa gcacaattga cgatatgagc ttcagttccc ccaaaaaccc tgtaatcaaa
     2701 gcgcttataa gtagctataa tgatattgtg gagaagtata taaaagagaa gacaattgga
     2761 tgagcttata gctcacccaa tagttttttg cctctttcat agaactcctg catcatagcc
     2821 tcaggtacaa acaaccctcc tgtagcccat gcaatatgag tcgcattttt catgctgacc
     2881 tcaggcaggt tggcattaat gtaagcctcc gcctgcaggg ggcccagcag ccccggcgta
     2941 gccgaaggct caatcttgat attctcctca tccttcatca ttgccagcag cttataaagc
     3001 tcattatcct caacagtata aataccgctt aaaagcctcc ccaccatatt gcctacaaag
     3061 cctgaaggcc tcccaactgc aagtccgtcg gcatcggtta tgttatctat accgaaatcc
     3121 tgcaccgaca gtttttcatt ttcgccggta gccagaccga gaagcataca tggggaatga
     3181 gtcggctccg caaaaaatac atgtgcatta tctccgaaga catgcttcag gccaaagcat
     3241 attccacccg gtgcgcctcc aaccccgcag ggcacatata caaacaaggg attgctctca
     3301 tctacctttg ttcccaaagc atcaagctgt ttcttcaaac gaaaagccgc cgcgctgtat
     3361 cccaggaaca agctcttgga atgctcatca tccacaaagt aactgttcgg atcctgctgt
     3421 gattgccttc tcccttcctc aaccgcctta ctgtaatcgg aagagtattc tatgacattg
     3481 actcccctgc cccttagaag actctttttc cattcctttg catcagcaga catatgtacg
     3541 gtaacattaa aacccaaggc agaacccatt attcctatac tcagccccaa gtttcccgtt
     3601 gagcctacag ccagggaaaa tttaccgaag aattccttga actccggcga ccccagcctt
     3661 gaatagtcat cgtcaatacg tatcaaaccg tgcttgacag ccaaatcctc cgcatgcttc
     3721 aggacctcgt aaatgccgcc ccttgccttg atagatcccg ccacaggcag gtaattatca
     3781 catttcagca ttaattcgcc ttggaagtcc tgccggtata ccttttgaat actatttttc
     3841 atttttccta tacataccaa ttcggattca atcaagcctc ctttgtccgc cgtctcggga
     3901 aacaacacgg atatgagggg tgcaaatctt tgaagccttt tctcagcatc aaccatatcc
     3961 tcaagggtca atgaaagctc gggtaacact ttgtctgcgt tgtccagatt cttgttggtc
     4021 cagaaaaccg gcttgcagct cattagatta tctatgatag gaaactcttc aatccattgg
     4081 cctatggttt tacctaatat tattttatcc atttgatatt cctccctata tttattttca
     4141 tactttcagc attacctgac ttctaaatat attgcatatt ttaattataa cgcatcaaca
     4201 gaaaatttca catttacaaa aattttctta ccgctgcata tttaacaaga cgaaatctgt
     4261 ttcacccttt ataaattctt tgctgttaca gtgtcaaata acccgctgat acataaaatg
     4321 ataatatcac tgtatgcgag gtgaatttta tgccgcttgc taccagagaa atatttgccc
     4381 ggatgattaa gtgtgaagcc ggtggcgaag gggacaacgg tatgaaggct gtagccaccg
     4441 ttattatgaa tagagttcat gtacccaacg gggaatatca aagaatcggc caaggggatt
     4501 taagaaggat actgttccag ccaggccagt ttgactgtgc ttcagatact ctcggaggag
     4561 catataatgc acagaatata tataacatgc ggcctgacca aattcactat gatatagccg
     4621 attgggctct gtcgggcaat aagatgctta cagtggccta ttgcctgtgg tacttcaatc
     4681 cttttactca gacatgtatc cagacttttc cgagaaacgg ctcaggaaca tttgccaatc
     4741 gaataggaga gcactgcttc tataatccta caaggcttta ctatactacg tagaaaatcc
     4801 gtccatgcgc gcttcgcgcg gcaaagtaat gcaaggacat gaaatattat ctatgaaagg
     4861 agataactat gtacagcaat agttcataca gttttaacca gacacctgca agcagcggta
     4921 accagtccca gttcagccaa gctcccgaaa tgggcaccac ctttggctat ccaatggcgc
     4981 cccagacatt tccgatacag cagcaaatgg gggcacaaca gatgggcggg cagcagatgg
     5041 gacagcaaat gcctcagatg cctcaaatgc cgcctccaac aatgcagatg cctgtgacag
     5101 gtgccgctcc ggtagatttt gacatgctgc cgacaacacc ggtacttgat atcgagtata
     5161 cacagggcta tctcaagact cagataggca aacgggtaaa agtggaattc cttatcggaa
     5221 ccaacatgct tatagaccgt gaaggaacac tagttgatgt tggtataagc tatatcataa
     5281 taaacgaagt tgagacagac gacctgctgc tttgcgatat ttattcgata aaattcgtac
     5341 gtttctattt ctaatatggc aggtgccgca taggaagaat tatgcgatgc gaggacacgg
     5401 ggaggacatg gggacgtttc tcctgtcccg ctgaaagcgg gacaggagaa acgtccccat
     5461 gtccccaccc ccgtgtcctc tacttgttaa attgcagcag ccttctttcc ccttcaagct
     5521 ttataatgtc cgttatatcc tggctggctt ccagagtccc caggtatctg cccactgaat
     5581 cccttacggc aaaatactgt atcagtatca tcctgccctt aacctttatc cagaatacgg
     5641 cattatcctt tctgccttcc ttaaaggcgt caagtatttc attgactaca tggacacttt
     5701 ccggagggtg gcatttttcc acatcccttc ctattaccgc aggggaacgg gggaaaattc
     5761 tatccttggg ctttgtaaag aattttactt tattattctc atcgacaaat gtcagatcca
     5821 caggcagtgc atcgaatatc gcaagaattt cattaaaatc caaagctcct gtttccgttt
     5881 caaagcggca gccttcctta atgcccggta tatactcgtt gtctgccggt tcaggccttt
     5941 caatgaacgg gaactcatat tccatactct gcctttgcat ttcaagccat tcatcatcgg
     6001 taataatctc actggctgcc gggaaaagga taagctcttc cttctgtacc aggccgtaca
     6061 tgtcaaagaa cagctttccc agctctgagt taaactcccc ttctatgcag ccttcagcct
     6121 caaggcattc aatgaccttt tttagctgtg ccctggcctc atcgtgcaac gcccacatga
     6181 tagcaaggcc gtcaaacttc ttcatcttct tttccaggta ggggaacagt atattttctt
     6241 ttttgagata gtggtcatta aacttccgga gttcatgcat ccggagtatc agttctttct
     6301 ttccggcctc cgaagacata tccttgatga tttccttaat gccctcaagt ttttcctcca
     6361 gagcgcgatt ttcccgcatc aaataatcca taaagctgtc tttgtcgggc catttccacg
     6421 tataattcga caggctttta taaaacacat ttatcgcttt atccagaaca gtcaggattt
     6481 ccttcggctt tatacccttc tgcagaataa ggtggaaaat ctcaaaaacc tcctggggag
     6541 taaccctttc aatataagtc ctgtacttaa agtatagctc ccttccattc tccttatcca
     6601 aaagcccctg tacgtattca gtcaaactct taattctatc ctgatccgtt ttcataaaag
     6661 tactcatctc ctttcaaaat ttttttgcat tgcaatatct tctaccgctg catacggatg
     6721 cgaatacctt ccgttttttt cagtttgcga ttttatctgg gcagactgca cagggcaata
     6781 gttgagacag gcagcacaat gataacattt cgcattatcc tgccattcag gctttccatc
     6841 aactatttta atcttatgcg caaggcaaac cttttcacat atgccgcatc ctgtacattt
     6901 cgaatcgcag tacaaattgt ctttggatat gtctataaga gccgccaggg atgaaaggat
     6961 ccccacaaag gggatatgga ttgtgcagtc aacatccttt tccctgctaa tttctttatt
     7021 ttttattatg ctctgaatag aatcaagcct tttttgaaca actgcttcca atcttgctat
     7081 ttcttcttct gtaggaacct tataattaaa tttcggatca tttccgggca tattgaggga
     7141 aaaatatgaa tccaggcgtt ggcctttgct tttcaatatt ttatctatgg ccataaaagc
     7201 actgtgtgaa gttccgattc ttgttgctgc agcaaaaata tattttgccg atttcaaatc
     7261 cagttttttg ataaaattct tcaccggtac cgggattgcc gaaaaatata ccgggaatac
     7321 aaaccctatt gtctctgcat cagtttcaac tacttctcta tccaaccggc ttataaccgg
     7381 tataagcttt gccccctcaa tccgcttctg caattctttt gctacataca gtgaattgcc
     7441 ggtaccggaa aaatagtaga ttcctatact actcattata actgctcctt tttatctgtt
     7501 cgcactttgc agtgcgtttt ctatggcctt taatattacc ttgctgctgt atgttgcacc
     7561 tgagacaata tccaccccta aggactgcgc ttctgtaacc ctgtccggga ttacttcagc
     7621 ggctgccccc tggccgttaa agtgcttgac cagctctatc gcgactatct tatgattctc
     7681 tattgtcacc cttacttcag cttctaccgg gaaaacctta tagctgcctt tatatacccc
     7741 atccgcaatc tttgacaaat ccacatccgg tatgcttgtt tcagccagtt ttacaaggct
     7801 tgcttcagta tatgatttga acccgaatac agcagttaca attgctgcaa gggctataac
     7861 cacagccagc actactcttc tcctgctttt catttatttc cctccttaca ttcacttaat
     7921 gacataaaca atccggaaaa cttctcagcc agattatcaa tcattccttt tcgatgtatg
     7981 ttggaccagt ttacaacacc ggttcctata acatcaaatc cttttgagct gcatatctct
     8041 ttcatccgtt cgattgcttg gtttcctccc atttggggaa gcggaaaaaa ctccgtcaca
     8101 taacaagctg cttttttatc cggaatcgcc ataagctgtg tcaagtaagc ctccattacc
     8161 actgaaggcg agaatccatg caccggagcc ccaaatatca atgcatcgta aggatttata
     8221 tccggcttgc ttttcaactg tacttttata gcatccattt gtccgttatc acatgcctct
     8281 acctgttcaa tacttgcaat atgccctttc gccgcaaggg ttttcattag tttctccgct
     8341 acagaatacg tattcccggt ttttgaatga acgataattc caattttcat atttgacccc
     8401 ttcccaatat atattcaatt actttgtcca gttcactata aacatcttct cccttataat
     8461 ccacatcttc tgaaaagtat aatgttatca gcccgtgtat ggcataaatc attgttcttg
     8521 caagtacttc aatgtcctct gccctaatct ttcctgaaat cacgaattcc ttaaaggttt
     8581 cttcccacat tgcatcaaaa tcgggctcct cctcattttc tccggtttct ccggcaggtt
     8641 tactcaaatg atggaaatag aaaaatctga acacattagg gtttactaaa aaatattcaa
     8701 catacatttt gaagcacttt ctgattccat cgacatcctt cgtcattcca gccatttttt
     8761 tgtataatgc ttcaacaata tcattgatca tcagtttctt aacttcccaa agcagttggt
     8821 ttagatcgga gaaataatta tatatggttg catacgaata gcctgccata tctgcaacct
     8881 tcctcactgt gacactttca acgccgtcat ttaatattat ttctctggct gcttccaaaa
     8941 agtatgtcct gatcctctgt ttcttcagaa tctttttatc ttccgctgtt ttctccttca
     9001 taatatcacc tatccccgtt tatataatct gcagtattaa cacgtataca tattattaac
     9061 attgttaata tttaataaca acgttaataa tatcatggtt tttattatat gtctatagca
     9121 aattacaaat tttttcaaaa aaatttaaga gccggaattt ttccgactcc cgtcacttat
     9181 ttaagaataa ctgactccta ttcggtgtct tcagaactct ccagcgacat cataaggaaa
     9241 ccgcagaaag ctccattatc aacctgaagc gaagcatcaa cctctttttt tatctgattg
     9301 atttgtttcc gataagcctt atagaattca aaggttggtt tgggactgta tttcaacccc
     9361 tccatttatc tgcaggtacc ggatataccc tatatatttc aattgatgac ttaaaaactc
     9421 cttctctaac tataagattc aaattacttt atcatataaa aatccatccg ccataaaatt
     9481 attgaaaggg ggtatttttg tggatttaaa acgctggcaa attattgggg cagttttcgc
     9541 ccttattatg ggagcacttc tgcattttac atatgaatgg tccggtgaaa acccggttgt
     9601 gggaatcttc agcgcagtta atgaaagcac ctgggagcat ttaaagctgt tgtttgttcc
     9661 ggttgctcta tttgcagccc tggagtatat tgggtatgga aagcagattc ctaactttgt
     9721 accggtaaag gtgctgtcca tcctgctggg tatgatcact atcataattg cttattatac
     9781 ttacacccac atagttgggc aaaattatct ctgggtagat attggaatct ttattttagg
     9841 agtacttgct gcatattggt tcagctatag tcttctgcag acagaatttc tcgcctcaga
     9901 gtgtgccgta tgcttgggtt gggcaggatt gctgctctta gccttttcct ttgcattttt
     9961 cacattttgt ccgcctcacg tcgaactatt ccttgaccct gtaaccggaa cttatggcag
    10021 atagctaagc taataacata atagccaccc gtgcggatta tccatagtcc gttgtaattc
    10081 tctttaatca ttcacccata atccgtgctg attacagcac aaataaatct ttccgcggta
    10141 cattttggga aaacgtactt cgctgccttg ttccgggtac aatttgacaa agagcactct
    10201 atcgtaagca acgtacgcta caaaattgat gtaatgcgtt ttgctcatct catgtgcaaa
    10261 tgtaatgtaa aaatcctcct ctatctcttc caccgttaat ttgtgagttc cctcagccgg
    10321 ttttgggata agcggcacca gttttcgccc acagcaggaa acatcggact cccctgtgga
    10381 agttagtgtg tttccgcaat tggggcacat ataaaatttt atttttttca tatttcctcc
    10441 gtctgcatta ttgggttcca aatcccccaa taatattttt tcgatgtctg cgtttaatac
    10501 ttcagagagt tcacgaagaa gggatacatc agggcatccc aaaccacgct cccattttga
    10561 aatggtcttg tcactgatat tcatagcatc ggcaagctgc ttttgtgtca tacccttttc
    10621 ttttcgcaaa ccgaatatca gctttccaac cttgctgcaa tccatattgt cacctccacg
    10681 cattaataat aatagcagac ggactgtaat tcaataaacg ctccgtagag tttctatgta
    10741 agattaaaaa cctatggatt ctccacttat aactgtagat gaactgcccc ctatcaagct
    10801 cctctttcac ctgttcggcg tctattctgc ccttgtcgat atttatatca ggctctgaga
    10861 ttatatatgc ctttccgatc ccaatacccg gggatacagc cagacctttt ttcattctcc
    10921 catcagcctt ttaccctaat tatctctcta agcaattcca atccccctat tcatcacggc
    10981 cgtccttaag aaaactgccg gaatctccgg ctattattac tcccatttta cgactctctc
    11041 atatactttt ttacccttta caaaggtggc atgtatgttg aagctatcat caaacaacac
    11101 aaagtcagca tccttgccta tgtctatgct tcctttcctg tcattgactt tcaataccct
    11161 cgcagggttc tcagtaacgg tttttattgc atctatcagc cctattccgg tattggccgt
    11221 aaaattcctc aaggctttgt ccaaggtaag gacgcttccg gcaaggttac ctgcaggcag
    11281 cctcgctacc tcattcttca ctactacccg ctgccctccc agttcatatt ctccatcctc
    11341 cagcaggcat gccttcatag cgtcagtcac aagaattatc ccttccactc cctttacctt
    11401 cagaaggatt ctttgtactg ccggattcac atgtacattg tctgcaataa gctcacagac
    11461 aacagaactg tccattgccg ctcccacggc ccccggctcc ctgtgattca gcggagtcat
    11521 tgcattaaag atatgggtga tatggcttgc acccttctga atcgctctca tggcctgctc
    11581 atagctggcg ttcgtatggc ctatcgccac tactatgccc tgactgacac agctttctat
    11641 gaactcctcg gcgccctcct cctcaggagc aatggttaca atcctgataa catcggacag
    11701 gccctcaata gttttaaagt caggccttct tatatatttc ccgtcttgcg cacccttata
    11761 ttcttcgctt atgaaagggc cttccaaatg acagcctagt atggacgctc ccggagcatc
    11821 gtccataagc gtccggattc tgtttatgct tttctcaatc atcggaaagc ccattgtcat
    11881 tgtagtaggc aggatagccg taacccctgt agctgcaata gccttgctta tgttcctgag
    11941 actgctctca ttgtcatcca tggtgtcatt tccaatacag ccatgtatat gcacatcgat
    12001 aaaacccgga gaaagatatc ttccggccgc atcaaagacc tcctccggct ccaggccctt
    12061 taatgcattg ccttccatca gccgaagtat tttctcttcg aatagcacag tatagccatc
    12121 aaatatacta ttatctttga ttatcctcgc attaactatt gccttcattt aatcaccttc
    12181 tatttttgaa ctgtcattta caagtgacaa tatcgcagcc ctagcattac cccccgcctt
    12241 ttcaaaggcc ttaagcgctt cctcctcttt ccagccggaa acagcctcaa tcctacccga
    12301 tattacacca attatcacat tatcatctat tccaaaggta ggagggcatt cggaagcatc
    12361 aattacagca acctttttgt cctcattatt aaacagtgtt accatttcaa ttgttgacag
    12421 cttatctatt ttttcagtat tagcatttat acgcaccgca gtacctcctc cggtcttaaa
    12481 acaaggcggc ataagccgcc ctgttgtctg ttaccttttt atggattttt tcatttcatc
    12541 agctatcagc tctgctcttg ttcccactat attggccttt cctccaagtc tttccaccag
    12601 tttagcagaa aattcactga cattcaagct gcccatagcg ctttcgtcca tttcctcatc
    12661 tacccttccg ggagtaggaa tgtcaagctt ctgaataacc caaacaaata ttatgtagta
    12721 tgccaaacca aacacaaggc ctattggaat tatcatcacc ggattggttg ccagccccca
    12781 gttaagcgca taatctatga aacctgctga gaagccgaaa ccatgcagta ttccaaaggt
    12841 atatgatact gcaagggcta atcccgtaag cactgcatgc agtacataga gcaccggtgc
    12901 caagaacatg aacatgaact ctattggttc tgtaataccg gtaaggaatg ctgtaaatgc
    12961 aacagagaat agtgcaccgc cgactgcctt cctgttttca ggctttgctg tcctgtatat
    13021 agccagtgcc actgccggca gggcaaacat gaaaatgagg tagaagcctg tcataaagtt
    13081 gcccgcgcag ctgcagttac tatgggcaca aaccttctgc atccgaaaaa cccgaggaac
    13141 tcagtcagct tgttattata gtatccggca tatttcaagc cataattttc cataataatt
    13201 aaaaagaaaa gaacctcgct gccttaagac aacaaggttc ttttctttta atatctattt
    13261 caagtcaaag ggctttaata atttttcaac ttcaatcttt ttgccctgct cgtccataac
    13321 cggaaccttc gcaaaaaccc agccctctac cttgttcgga tctgcaccgg catctataaa
    13381 tggctgcgaa tcaaactcca gcataacgtc cggtgtacct tcactgctga aatccttccc
    13441 ccagatgaaa ctttcgccgg tcggggaagt caatgcccag ccgttattca cttcatccga
    13501 ggttatattt tccggaattt tatttaacag tgcttcgaag gatgttaccg caaatttgcc
    13561 tactacatct gttgcttaac acgctgccag ggataaagct gcagtcaaaa ctaaaataat
    13621 aactaagtat tttttcataa ttaataacct accttcttta tttcagcatc ttttcaattg
    13681 tataaagcag ctcgtcgacc acatccagtt ccccggcttg aatacgtttt atcaggcagg
    13741 atttcatatg atttttcaaa atcagtttgc ccaccgaatt cattgcagag ttaacagagg
    13801 atatctggtt cagaacatcg tcacaatata catccttctc tatcattccc ttgatgcccc
    13861 ttacctgacc ctcaatgcgg ttcagcctgg atatgatatc cttcttttct ttctctgttc
    13921 ggacagttac attgtggtca caattacaat ccaagggtat acctccttta ctgcttattc
    13981 acagcacttt tacagcgccg gcatcatagc cggcttcatc gactgcattc ttaagcacag
    14041 aatcgggaac ttccttttcc aattccacaa ttgcgtattt gccttccaaa ctcacatcga
    14101 cctttattac gccgggaacc tctccaagtg cgccctcaac atgcttaacg cagtggccgc
    14161 agctcattcc ttcaatatta atttgttttt tcatttttat tcctccttaa atttctattt
    14221 ggtattcctt tcgggttcgg ttgcaaattt cgggttacgc gttacgtgtg gctcaaatct
    14281 caagtcccat gcctacttat atggtttaaa gcctttcagc cttaaggcgt ttgtcaatac
    14341 cgaaactgag cttaacgaca tggctgccgc tgccagtatc ggatttaaaa gcgggcctcc
    14401 aaagacgtga agcaatcctg ctgcaaccgg tataccggct acgttgtagc caaatgccca
    14461 gaaaagattc tgcttgatat tccttattgt actcttgctg agctgaatag cagtaggcac
    14521 atccatcaaa tcgcttctca ttaaaactat atctgcagat tccatagcca cgtcggtacc
    14581 ggagccgata gcaatgccta tatccgcctg tgccaatgca ggtgcatcat tgattccgtc
    14641 accgaccatt gcaaccttct tgccctcaga ctgcagcttt ttaacttcat tagccttgtc
    14701 ctggggaaga acctcagcca gtacccagtc gattccaacc tgctttgcta tagcctccgc
    14761 cgtcttgcgg ttgtctccgg taatcatgac tacttctata cccatttcat gaagcttatt
    14821 tattgctttg gcgctgctct cctttactat gtccgcaact gcaataatgc cggcaagctg
    14881 attgccaatt gctgcataca tcggcgtctt tccttctgaa gccaatctgt ctgattcggt
    14941 ttcaagcccg gaaagactga tattcctttc tgtcataagc ttcctgttgc ccagtaatat
    15001 attcttccct tcgatctcaa cctcaatacc ttggcccggt attgcattaa acttgtcaac
    15061 cttgagatct tccaggtttt ctttctctgc cccgcggact attgcttccc cgaggggatg
    15121 ctctgagcct ttctcagcag atgcggcaat ctgaagaagc ttgtccttct ttatgatttc
    15181 tgtggtaaca atatcagtta cctccggcct tccttcggtt atcgtaccgg ttttgtcaaa
    15241 tactatcgtc tttattttat gtgttgtttc aagcgcttct ccacctttta taagcactcc
    15301 gtattcggca cccttgcctg ttccgaccat aattgccgtc ggagtagcca ggcctaatgc
    15361 gcaggggcag gcaataacca atactgctat aaaaaccgtc agtgcgaata ccggtgtttc
    15421 cccgccgata aaataccatg ccagcgccga taaggttgct attacgaaaa caataggtac
    15481 aaaatatccc gaaacaatat ccgccatttg cgctataggt gcctttgagc cttgagcatc
    15541 ttccaccagc ttaattatct gagccagagc tgtatcgctt cctacttttg tagccttgaa
    15601 tctgattgaa cctgtcttgt ttatacttgc tgcgaatact ttatcccccg gctttttctc
    15661 aataggaatg ctctcaccgg tcagcatgga ttcatcaatt gccgtaaggc cttcaactac
    15721 ttctccgtcc acaggaattt ttgaaccggg ctttactgct ataatatcac ccacctctac
    15781 ttcctcaatg ggtatctcaa tttcctcacc cgcttgcatt actatggctg ttttaggagc
    15841 caggcccatc aatttcttga tagcctccga ggttcttcct tttgataccg cttccaggga
    15901 tttccctaaa agaatcaagg ttataatgac acctgctgtt tcaaagtata aatcctcagc
    15961 atacataaaa tgcccgatgc ttatctgata ggttgagtac aagctgtaaa gcactgcagc
    16021 tgaggtaccc atcgctatca atgaatccat attggggctt ctgtgcaata ttgccttaaa
    16081 ccctactgta taaaaacgat accctgcagt aactaccggt actgtaagaa gaatctgcac
    16141 caaagcaaac ctgagcggga aatccattgg atgcaaagct ttcggcagcg gcagtccaag
    16201 catatggccc attgctatat aaagaagcgg agcagcaaaa actgccgaaa caataaactt
    16261 tgtccataga attcggattt ctctgtcctt gcgcttttta tcctcgtcta ccttatcctt
    16321 gctctctatt tcaagagcct tatagcctgc ctttacaata gcttgcttta tttccgacag
    16381 cctcagctca gccggatcgt acttgacatt tgctttttct gttgcaaaat tgactgaaac
    16441 agcctcaact ccggaaagct tgccgactgc cctctctatt gattttgcac aggcagcaca
    16501 agtcatacct tcaatcggta ttgaaacctc cttggtgttt ttttcctgta aagcttcata
    16561 acctgctttt gaaacagctt cagcaatgtc gcttggtttt aactttgctt catcataggt
    16621 aagggaaagc ttttctgtcg ctaaattaac cgtagcatct gacactccgt ccagcttctt
    16681 aactgctctt tctactgctt tggcacatgc tgcgcatgtc ataccattta tcctgaaaat
    16741 atctgatcgc atcatatttc tcctttcgta tttactttac ccccctaccc tatctgtata
    16801 taaatataat atcacattat ggagatttta tgaagattca tttatttatt ttccccaatc
    16861 tccataaaat caacatattt gatatatata ctatatctca caagtacatt aaggaggata
    16921 gctatgagca ttaagaaaga aaaaatcaaa atttttgaaa tgacctgcac ctcttgtgaa
    16981 aaccgtgttg aaaaagccgt caggcaatta gagggggtta tatatgccaa agccagctac
    17041 agcggccaat atatggaaat tgaatatgat gatgatttat gcggcatgaa taatattaaa
    17101 gatgctgtaa aaaatgccgg atacagcaca gaaggttcta aggactgcaa attcatgggg
    17161 atccttataa ttgcggtggg tgtagtgctg ctgggattaa agaccggtgg ttttgacatg
    17221 gaggcaaaac ttaccaatgc ttcctatgca gttcttttca ttgtaggggt tcttacttca
    17281 cttcactgtg taggtatgtg cggcggaatt atgctttcac aaagcttgtc aaaagaaagc
    17341 aaaaacaggt tcgaagccat ccagccggct ctgttatata atatcgggag agtcatatct
    17401 tacactattc ttggcggtct tattggcgcc cttggttcag tatttacgct ttctattgct
    17461 gcaaaggccg ccgtgcagat atttgccggt gcatttatga ttattatggg tttaaatatg
    17521 gcaggcttca gcgctttcag gaaatttcag ataaggcttc ctcaatttgc atgtaaagca
    17581 aaatcaaagt ccggctctcc gctaattgtc ggccttttaa acgggctaat gccttgcggc
    17641 ccgcttcaga ccatgcagct tttcgctctg ggcacgggaa gtgctgcaaa aggggccctg
    17701 gcaatgtttg tattttctat aggaacagta ccccttatgc tgaccttcgg tgcactatca
    17761 ggactgctca gcaaaggtta taccaagaaa atacttaaat tcagcggtgt gcttataata
    17821 gttctcggcc tgatcatggg taaccgaggc ctcgcgctgg caggtataaa tattaatcca
    17881 ataactgcct ttgcaagcaa ttcagcaggc tcttcaaatc catatgttgc gaaggcgact
    17941 ttacaggatg gtatacagat tataaatatg actgctgata ataacggtta tgcgcctaat
    18001 gctttttatg tccaaaaagg aataccagtc agatggataa ttgacggaaa agaattgaat
    18061 tcctgtaata atgcgattgt tgcacgagga ttgaatcttg aacggaaaat taaaaaggga
    18121 gaaaacatta tagaatttac accgggggat aaggatataa acttcagctg ctggatgggt
    18181 atgataagcg gtgtaataaa ggtcgttgat aatcttgatt ccgtagatac ctcgaaggct
    18241 gatccgtccc tgcctccccc aagtacaggc ccaagctgct gtgcagtacc gattgaagag
    18301 gatgaaccaa gccagcccag catatacggc gatgatataa gcaaggtgcc cactgaaaag
    18361 ttagttaata aagcactggt agacgggaag aatcaaagtg tgatattcaa aggtatcggt
    18421 tatgaattac agcctttgat agctgttgca ggcagcggcg ggaaaatcag gctgaccctt
    18481 gatttaaacg attttgacaa tgccgaaggg gagtacctga taatagatgc ggtaacagga
    18541 aaagatgtta cggcttttac tgccgaaaaa ggtataaatg aaatcgagtt cagtccaaaa
    18601 aatgttgatg gttatgcaat tataaagggt aattctatac taggcataat tgaggtagtt
    18661 gacaatcttg aggctgcgga tataaaagaa atcagggaga agtatcttcc gtagaagaat
    18721 aatgcgacgg ggacatgggg gcggggacat ggggacgttt cccctgtccc acttccagtg
    18781 ggacacgcga aacgtcccca tgtcctcccc atgtcctcgc taaaaccttc cggaagcgcc
    18841 tccgcctccg gaacggcctc cgcctccgga attccttccg ccgcctccgc cgcgtctgcc
    18901 ggacgaagca gccattctaa ttatgaagaa ggttactctg cctccggtaa atataaaatc
    18961 caggactatc aaaatcaata taatgattac aagtattaca gttataatag ggttctccct
    19021 gaagaagctg aagtcatcac ttgtatactg cctttcttcg ccgatttcag ggtaccggac
    19081 catatcttca ttcccgctta cgatattggc tatatcggaa taaattgttt taactgcttc
    19141 gtccatgcct ttttcttcat aaagtccggc aaacttcaca agcagcttgc tgctttccaa
    19201 gtctgtcaat acgccttcgt agccataacc tacttcaatt ctggattctc tctctttcat
    19261 actgagtatg aaaagaatac ccttatcctc agtgcctatt ttccatttgt tgaaaagagt
    19321 attggcatac tcttctatgg tatttccgtt taatgtatcg atactgacaa atacaacctg
    19381 tcctccatct gttgctttgt aggtattttc ccccagcgcc agaatatccc gttccgtgcc
    19441 ggaatccaga ataccggcaa aatcattgac ataaaactcc cttgtaggat tcggtatttc
    19501 aggttcagct ccctgaacga ttattactcc aaagctcgtg acaatcagaa gcaaaactgc
    19561 aataaatgct gcagacaacc ttaattgttt cactcacatg ccccccatct cgaagtcttc
    19621 aaatagatga acaactctaa aactatgttt tagagttgca gtatagttcc tttcacattc
    19681 cgaattaatc aaaattcaca tcaggtgcct gctccgcacc ggcttccgct tcaatataag
    19741 ggaattcatc cgaataaccc aatatgcctg ccgctatatt tgtcggaaat acctttatct
    19801 tcttgttata ttcctttaca ttgtcattat aatattttct ggcaactgca atcctgtttt
    19861 ccgtgccttc aagctgtact ctcaagtcct ggaagccttg attctgcttc agctccggat
    19921 aattctccgc gatagccaga agtcttccca atacagacga aacctccgca ttggcatcaa
    19981 ttttctcctg tatgtttgct gcccctgaca gctttgaacg cgcttccgca atctgtgtaa
    20041 aaatttcctg ctcttttata gcctgtgctt ttaccgtttc aacaatgttt ggtattaaat
    20101 ccgctcttct ttgatattgt acttcaatgt cgctccagga tgcgttcact tcctcgctca
    20161 aattcaccag gcttctttgt acccccacaa cataaaccgc caacaagacg acaatcccaa
    20221 ttattataat ccatgatttg tttttcactt tactacctca cttatttttt tactctgaat
    20281 ttctaatggt acctataaaa ctcatcataa tagaatatac catatttatg gtttcattga
    20341 aagacggata ttatccggat atttaccgaa tcaaaatccc tttattgaat tcctttgaac
    20401 ctatcatctg cacactgcaa atatagctct agctccttat atctcccttc gattatcttt
    20461 ttttcggcag agcaacggta aataccgtac cttcatttaa tttgccggat gctgaaatgc
    20521 tgcctttatg agcctccaca atagccttgg caatagtcaa tcctatgccg gagccgccgg
    20581 tcgtcctgtt tctggactta tccgacctat aaaaacgttc aaatatatag ggaagatctt
    20641 cttctgagat accgataccg gtgtccctaa ctgcaattgt cgtattatcg ccggaatcag
    20701 aggcggttat tgtaacctgg ccattgctgt tggtatactt taatgcattt gatatcagat
    20761 ttacaagaac ctgactgatt ttatccctgt cagcaaaaag cataatgttt tcacactgta
    20821 tttgaatctt tatattcctt tgtataaact ccttttcaaa gttctgtacc attatcttta
    20881 tcaattcgca taaagaaaac tcttcctcat tcaatatcat attgtcgctt tcatagcgtg
    20941 ccagcttttc caggtcaccg atcatcctgt ttatcctcat tgtttcctca tggcagcttt
    21001 tgagcctgtc ggagcttggc tcccatattc cgtcaagcat tgcctccagg tggctttgca
    21061 gtgtagcaac aggtgttcta agttcatggg caacatcacc cgtcaggcgc ttccgcagtt
    21121 tctcctgggt ttccagggtt tccgccaaat catttatagt atctgtcaac tggctgattt
    21181 cttttgtact tgttcgttca aagcttctgt ccccgaaata accctttgct atcatccttg
    21241 ccgtgttgat aacccgggtt ataggcattg taatcctcct ggacataatt gagccgaata
    21301 aaaaggccag aagcagcgaa aagacaccta cactaataaa tatacgatta agtgtgctta
    21361 taaatgccat atcattgtca ttaaaataga aagggccata atacccgata tctatactgc
    21421 cgactttttc tcctttatag aaaacgggat agttaacctg caccagctta ccctcccaat
    21481 tggggtaacg gctgtacata ttatttgcca tatgatcaag catttggtta caccaaccgt
    21541 tattatagga catggcattc catattatat tgcccgattg atcccttact tcaacaatca
    21601 ttccctgctc aagggcatat atccctatat cctgtacgct ttccgcattc cactgcccat
    21661 tgctttgata attgctggaa aagagcgaga ccaattcttt attctttttt tcctgatttg
    21721 acctgatgta atcctgaaag tgcctttcca gcaggatatt ggaaaacaag cttatcaatg
    21781 caacgcatat caaagctgtt gcaacatggg acaatgtcag tttagtcctt aaggatacct
    21841 tcaaatctct caccgccgaa cctattctct gctatcattt ttgggcctgt ccaagttgca
    21901 gcagtccagc ttttcgctgc cgaactgctc cttgttttgg gctgccagcc tcttcaggaa
    21961 atcatcaatt atttttttca gatttttcat aacttaacct ccccgtacta tctccggttg
    22021 tatcaagata cggcatttcc cgtaattctt ataatgttca aatttcaaca tatgctgttt
    22081 cattttacat tatcatatta gcaagaaaat atgaagaagt tatgaataat ataaaagttc
    22141 ggcgctgatt ttgctatcgc agaatctttt ttatctcttc ggtgtctgca actctgccca
    22201 taaccttcac ttcctcattt acaacaagtg caggtgtttt cattactcca taggccagta
    22261 tgtctttgta attttccact ttctcgactt ttgcatcaat acccagttcc ttcactgccg
    22321 cttttgtgtt ctcctctagc ttcctgcagt tcatgcagcc cgaacccaat acttttatta
    22381 ccattttttg acctccttaa aatatttcta ttatattaaa ataaaagaaa ccgcattgaa
    22441 taaataccct attatcacaa taccgacaga cactattcca gcaaatattg ccagaagctt
    22501 cggctttaca accttactca gcaatatcat cgaaggcaaa gacaatgcag taacagccat
    22561 catgaaggaa agaacggtac ctactcctgc acctttggta aacagtgcct ctgcaatagg
    22621 aatagtgccg aatatgtcag catacatcgg aactcctata gcagttgcca ataaaaccgc
    22681 aaaaggattg ttttctccta tgacattttc cactatgctt tgaggaatcc aattgtggat
    22741 tgcggctcct atgccaacac caatcaatat atacagccag acttttttaa taatatcctt
    22801 tacctgctgc tttgaataag aaattctttc atggcgggtc atttccgaca tctccaagtc
    22861 gacattctct atatctctta cataactctc aacgtatttt tcaagtccca gcttgtcaat
    22921 gaccgtacca cctgcaaccg caagtatcag tccgacaaca acataagcaa ttgctatctt
    22981 agctccaaag aatgacatca gtatcagcat tgaagctaaa tcaaccaatg gtgatgatat
    23041 taaaaacgag aatgttacac ccaacggcag cccggccgag gtaaaaccga taaatatcgg
    23101 tatacttgag caacttcaga agggtgttat agtacctaac aatgctccta aaatgttccc
    23161 cttgatacct ttgaagccac caagtatttt cttggtcttc tcaggcggaa aatagctctg
    23221 tatataggat attgcaaata tgagtacaga cagaagtata aatatcttaa ttacatcata
    23281 tataaagaag tgaatgcttc ctccaagcct tgtttcgacg gataggccaa ataccttctc
    23341 caccaaagct tcaaccagct ccgacagcca cgtcatttta agcagctgag catttaacca
    23401 actgaatatc tgagtaatat agctcattga tctcacctcc cccaataaga atacaaatcc
    23461 cagtaatgca aaggatttat aactttagca gcatttattc tctccacttc cgcagcatga
    23521 acttttttta aaattatgat tgattttgtt gacaacaacc tcaatacagg atgcctgcaa
    23581 tacattggaa atatcattca gcaaaccaat gccttgtact gtttgcaatc tttaccaatg
    23641 caagcataac cggaacctcc accagtaccc ctacaacagt agccaatgcc gcacctgatt
    23701 caagcccgaa caacgaaata gccactgcaa ctgcaagctc aaagaaattg cttgccccta
    23761 tcatcccggc cggggctgca atatcatgct taagcttcca tgcctttgcc cagccgtaag
    23821 ccaggaagaa tataagaaag gtctgtattg tcaacggtac ggctataagt gctatatgca
    23881 gcggattatt caatatgatt tctccctgga atgagaaaat gataattaat gtaagcagta
    23941 aacctattac tgtggtattg tcaaacattt ttaaaaaaac attgttgaaa tagtccaagc
    24001 ctttgttctt aattatatac ttcctcgaga aatagccccc tgccaaaggt attaccacaa
    24061 acaatataac cgacaggaat aatgtatcat atggaacagc aacatcactg acaccaagca
    24121 gaaaagccac tataggagta aaggcggcaa gcagtatcaa atcatttact gctacctgca
    24181 ccagggtata agcaggatct ccctttgtaa gatggctcca tacgaatacc attgccgtac
    24241 agggggccgc acccaaaagt actgctccgg ccaggtattc cttggccaat tcagcaggaa
    24301 tcagggcttt gaaaactaca tggaagaaaa gtgcagctat tgcatacatg gtaaacggct
    24361 ttatcagcca atttgtacca caggtaacta tcaggccttt tggctttttt gttgcgttaa
    24421 ctatacttga aaaatcaatc ttaagcatca tcgggtatat catcagccaa ataagtatcg
    24481 ctaccggtat tgatacattt gcgtattcaa atttgctcag ggtttccggc actatcggca
    24541 gtacctgccc aagaatgaca cccgcagcta tacatattgc aacccacact gtcagatact
    24601 tggcaaaaaa accaagggct tcattatccc tttctattga cttttgttta ttgttcattt
    24661 attttccctc acattcacat ccgcatttgg gtttttcttt acagatacaa tcatctgtat
    24721 ttgtaataat actcataaga aacagtataa gcttattgca gtttgtaccg ttcaatgaat
    24781 aatagttcca aagcccttct tttctgcatt ccacaatgcc gcactccatc aataccttca
    24841 tatgatgtga aagtgttggc tgagtaaagt caaaatgttt caatatatca caggcgcaca
    24901 tttccccgca ggacaaaatg tcgattattt ttagcctgct tggatctgaa agcgctttga
    24961 taatttttgc gttttcatta taatccagat tcacttaacc acccccttat atagatatat
    25021 atctatattt aatatatgtt aataccctca tatcgtcaat atatattaca taaataaatg
    25081 ctgaattaat acttatattt gcaatcctct atataaaacc atacacaata caaaaaaatt
    25141 tatagtttcc tattgatacc atcgtaaatt tacgatacga tatatcgtag aaaaacgata
    25201 taggggttga ttatatgaat atatcaagaa tctgtaatgc tttaggaaac gaagtcagat
    25261 atcagatagt caaatcactg aaaaacagat caatagctac ttgctgtaac agaattgaat
    25321 actatgaaaa cggcataagc gtcggtgatg tggtaaatgc tacagggctc gcacaatcaa
    25381 cggtatcaca gcatataaaa gtgcttctgg atgccggaat catttacaag gagaaaagag
    25441 gacagtggac ctgcttcttc ttaaacggag aaataatcaa taacttcatt aaaggacttt
    25501 cacatgactt gatccagact gaatgcgact gcaaaaaata acggttgaat tttttttaaa
    25561 taaatatatc gtaaatttac gattaatttt ggaggaagta taatgattaa tcttttaaac
    25621 gaaaccatca gctatgttgc cggcacattg ctccataatg ctccggtcct tgtcttcggt
    25681 atacttacag ctgcagccgt aaaagtatat gcagaccagg aaaaactaag aaaagtgctt
    25741 ataaaaaaag ccgggagctt tgcagaaaaa tacaaattaa aggaactttt taaagtattc
    25801 tatgaagtgg gtataaagca agttatatta tactttgcca tattcgccgc aataggtttc
    25861 ctgataaaca gattcgtacc ggcagaagta attaccaaat acctgggaac agacaacaaa
    25921 tttgcagtac cattaatggc tcttgtagga ttacccctat ttgtaagcgg ttcatcctcc
    25981 attcccatat taaatactct tatagcagga ggtgccagcc aaggagcact tctggctttc
    26041 atgataacgg gtcccggtac aagcgcaggg gtgattgcgg gtttagctgt gattatgaaa
    26101 agaaaggcaa taggattata tattgcttac tttctggcat ttggaatact ccttggatac
    26161 ctttacgatt tcctattaat gcttgttata tctccatttc catatcatcc tcaaactgcg
    26221 cattatacag ggatgcgtat actccgccgc tgctaataag ctcctcatgg tttccctttt
    26281 cttctatccc attatcggta agcaccagta ttgtctttgc ccttttgatt gtagacagcc
    26341 tgtgggctat cacaaaagtg gttctgctgc tggacaattc ctccaaggat tgctgcacta
    26401 tcctctcact ttggttatcc agggatgagg ttgcttcatc cagaatgagt ataggggggt
    26461 tcttcagaaa agccctggca atgcttattc tctgcttctg ccctccggaa agcttgactc
    26521 ccctttctcc cacataggta tcatagccct tttccagagt cattataaag tcgtgggcat
    26581 tagccttttt tgctgccgct ataacttctt catccgaggc cccgggcttg ccgtatttta
    26641 tgttttccgt tacagtcccc gcaaataggt aaacgtcctg ctggacaatc ccgatatttt
    26701 ttctcaggga cttcagtttg atgttcttaa tgtctactcc gtcaattttt ataataccct
    26761 cagaagcctc ataaaacctt ggtataaggc tgcagagtgt tgattttcct ccccccgagg
    26821 gtcccacaag agcaaccgtc tctccgggct caagattcag atttatatcc tttaatacct
    26881 ggcctccttc gccatacttg aaggaaacat ctgtaaattc aacccttccc tcaactttct
    26941 caagggatat gctatcctca ccgtccttta tgtccggttc cgtatccaat atttccagaa
    27001 acctttcaaa tcccgtcatc cccttttgga actgctcggt aaaatggata agggacctta
    27061 tgggcacaag gaaggtatta atgtagagca gatatgccac gagatccgag gttgctgctg
    27121 ttccaagctt tatgaaatat gcaccggcaa taacggcaaa aatataaatt ataccgtcaa
    27181 aaaattctat cccgctgaag aagcggccca tataaaagta actttcctgt tttgtgtcaa
    27241 gaaagcccct gttcccccgg gtgaacttct cctgttcctc tctttcattg gaaaaggact
    27301 gtacaactct gattccggcg atgctgtcct caagctgggc atttatttcc gccaccttct
    27361 gcctgttcct cctgaaaaca gagcgcatct tgttgttgta gaaatatgca aagcccatca
    27421 ttaagggtac aaaagcaaag gtaaccaagg taagcttcat attaatcctg agcagtataa
    27481 ggaaagctcc gagtattttt ataattgaaa tgaatacttc ctcaggcccg tggtgtgcca
    27541 attcgcttat atcaaacaga tcgttgacga ttctcgacat tatatgcccg gtcttgttat
    27601 tgtcatagaa ggaaaaagac agtttctgga gatgcgaaaa aatttcttct ctcatgtcat
    27661 attcaattct ggcacccatg atatgtcccc aagccgtgat aaaatactga cagaagtact
    27721 tgagtatgtg catcccgagc attaatgccc caatctttaa aataatatca aaagccacac
    27781 cgatttcaac atttatacca tgatccagca ggtatcttac aagcacgggg aaaagcaaat
    27841 ctattcccgc taccgtgagt gaacagaaca tatcaagaaa gaataagcct ttatagggct
    27901 tataatatga aatgaatctc cttatcttac tcataggtta aaacccccac aacaaaacaa
    27961 taaaactgca ctaagtaaaa aaacattgtt aacaaatata ttatatgcat ttgccgtatg
    28021 tattccaaca aatatttata ctgcttaatt tccatccaac acttttttgc aaagcatatc
    28081 agagatttca acctcaggat gatgatactt cacccctaca gttctgctcc aatgatgtat
    28141 cgcattgtac gaacaccatt cgtcacatgc aaagcacatt tcgcacttgt gctgaaattc
    28201 cggtttttta tcaacaattt taatattatt tacagggcat accttttcgc atgttccgca
    28261 gccgttgcat ttttcatcta taataatgct tttgtctgtt aatggaatca attcaaagta
    28321 ttttaatccc gtatccatcg gctcttttgc tttttccttc aagtcattta ttactgactg
    28381 ccgtatcagt ttatataacg gtgtcatcag caggccaaac aattttttta caattttata
    28441 tttggtgcct cttttattta tgatgcgata acaaacatcg tcaattttag ctttgctctt
    28501 attgattata atttcttgat ccctgattat gggaatagga atatgatcgt aatccaagtt
    28561 gttcatagga agtcttacgg aatactcagc cgatagtttt ccgccgcaga atcctattat
    28621 atgtctcaat ctttctaacg aaggaagtgc attaacgcat tcatatcctc cgcaagtaca
    28681 aaccgcaaaa atatatattg atccaatatt ggctattttc attacaaatc gctctactat
    28741 aaggggcact cccgataatg gagccaaata tgaaggaaag ataattccga tactttcggc
    28801 atcaacttgt attttgtcca tgtacatggc acccgggata gatatcaact ccgcatccgc
    28861 tttctttgca atatccttag ccacagcaaa tgagtttcct gttcctgaga aatagtatat
    28921 tttcatactt attgccggaa cctccttgaa ccttattgat caaaaaatac cattatcaat
    28981 tatattatat atttcctgca atcttatttc aatagaagga catggggacg ggggacacgg
    29041 ggacgtttcg cgtgtctcac tggaagtgag acaggggaaa cgtccccatg tcccccgtcc
    29101 ccatgtcctc atcggaatgc ttattcatcg ataagcctat ccgccatttc aaaaatctct
    29161 tcctgcttac ctttcggaag ccggtcaata tagtttttca aatattgccg caatgccatt
    29221 tccccttccg ataaatccac agcctgtata tcagttctaa acggatgcct tcttatcaat
    29281 gcggcaatct ctacatcttc aagaatatca aaaaccgaat ccgtttcttc ttcactgagc
    29341 tgtgcgtcaa acaataaatc ttccaagtct ccgaaggatt ttaccttttc ttttataagc
    29401 gcaagcttat cgggtgaatg gcggcacatg agcaattcgt caatcagctt tctgtaatcc
    29461 tcgtcatgca tcttcgtccc ggacagaaaa cggattctgg gtttcaagtc cggcaaagcc
    29521 ttcgatatga aggttttgtt cagagtattc atccttactg cgtgaaggat attggttata
    29581 attttcggca tgcttctttc tatatatctt tttaggggaa gactgtcagc attcagctct
    29641 tcaagcatct ttcctgctgc tttgcggagc ttcaaagaaa gtgaacaatc gtcgtattcc
    29701 gataattcat aatgaagatg ccggatttct tctgctgaaa tatccagttc cacaacgttt
    29761 cggtttgcaa gggagcaccc aagtgccgcg gtcaatacct gctcaaagat attaatcagt
    29821 aaatccttat accctttgtc gtatccgtaa agcatatgat ggatatcctc tggggcaaaa
    29881 cccctgcaga attcattttc cagaaaaaga ttctcaaggt atttaaggat aaattcgatc
    29941 cctatcatat tagtgacagg attacacagc tgataatcga tggatgcggg agtctcatgg
    30001 gcctcataat cggggtcata tgccttgaag aagcttccga taccttcgtt tattgttgag
    30061 ttataggaat agttgggtgt aaacaattta ttcttccgaa ccagattata aatatgtttt
    30121 gcggaatgaa gcctgacatt aatcagtttt ctgcccttct gatacatttc ggagacactt
    30181 gcctttttta tttcattgac agcgaagtca acatcgggca gcgacttcaa atatagtcct
    30241 attgtgtaga ggttggattt cataatgttt tcagcagcct ccactcttat ggaactgctt
    30301 tcaccgccgc tgtatctttc acatatattt gccaatagct gtatgcattg cagctgaatg
    30361 ttctccatgt cggagtcgca taaaagccca ttggcatatg ccccttgcag gattgaagta
    30421 aaatattgct caccgctcag cttattactt tcaatgacat gttgcttatc aatattcgcc
    30481 atcttcatcc tcctcatccg gcatcggatc ctccgaataa tccggaggat aaccgtatct
    30541 caaattacgc gccatattgg caagctcccg tccggaaagc agttccaggg atccctggca
    30601 tttgccatca aagctgtttt tcatgaattt tatcagatca tcatcgctca tcaaatctag
    30661 ggtttcattt ttgtaatagt aaaacatctc cgtaagctca tgaattgttt cagcatagtt
    30721 gtgcattgaa atatacgggg agtcacagaa ttccctgatt atcttatgga tggcaccgcc
    30781 cccgaattcg atccttccgt tgcttttcag ggcaatatta cgggcttcaa ccagttcagc
    30841 tgcatcggag ggggataggg ctaacccgaa ttcggctgtg aattcgttgc atctttcaat
    30901 ctctgttatc gcttgctttt ctatcagcga cgaagatatg ccaataatct caaatgacaa
    30961 aagcaatcac cccaatataa aattttattc tgaaaaactt ccaaaaagct attgacatat
    31021 tgactgtaat gtgatatagt ataatgggtg tattgtatgc taatataaat taaccgtatc
    31081 ttataatata ccacttttaa atgcaaaaag caatatactt actataatag gagcgatatt
    31141 tatactcgct ttttttatcg tataggaaat aataatttcc tatacgatgg gggatgcaag
    31201 ggggagaaac cccctgccgt ccaaggaggg tgctcaggat gcttgctaac gccttcggga
    31261 accatagggt tccctagcga aaaaaatgtg tcgtaagacg cattttttat tgctcatcat
    31321 acataaaaca aaaactgaat ataatgcatc ggattaaaca taag
//
LOCUS       DAOOAB010000031        30321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_1965_length_30321_cov_16.831164, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000031 DAOOAB010000000
VERSION     DAOOAB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 30321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30321
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_30321_cov_16.831164"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..788
                     /locus_tag="PKU88_07990"
     CDS             210..788
                     /locus_tag="PKU88_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HPX69252.1"
                     /translation="MITYEINDALYINLTNRCTNNCSFCIRNHPEGFGFDLWLPKEPV
                     LEEIMADLENLELYNEVVFCGYGEPLVRYREIIGICRHIKQKGVRTRINTNGQANLIW
                     NRNIVPELKGLVDSISISLNAKNAEDYAELCRPEYGKAAYSAVLDFAGECIKYIPEVW
                     LTVVDVISAYDIEVCRGIAEGIGAGFRVRHYC"
     gene            1026..2063
                     /locus_tag="PKU88_07995"
     CDS             1026..2063
                     /locus_tag="PKU88_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPX69253.1"
                     /translation="MKINLKDFKSLFSIKLIAVAAICLIFAASYVYSAMKKEITIEDI
                     GGVKVVSTFKETVSDVLEENNIQLIPQDKINLGMTEKLEDGTHIKISRAKKVTISSDG
                     EDIVILTTAPDVKSALGEVQIKLGEKDRVSPGFDTAITGDTTIAVTRVEERIVKQEMK
                     MDFTNEVRKSDNLDKGLARVIKKGSQGSKELSIKVIFENGKEVKREIAEEKVLKEPVN
                     GIIEEGTRTTFVSSRGQVTRFVRALKMTATAYDATFESCGKHPDHPQYGITYSGLRVR
                     PGIVAVDPKVIPLGTYLYVEGYGEALAADIGSAIKGNKIDLYYESPEDVAKYGKRTVK
                     VYVLDKPKYKF"
     gene            2105..2647
                     /gene="rnmV"
                     /locus_tag="PKU88_08000"
     CDS             2105..2647
                     /gene="rnmV"
                     /locus_tag="PKU88_08000"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HPX69254.1"
                     /translation="MLKETIVVEGKNDAAAVRRAVEADIIITSGFGINGSILERIRTA
                     QDRNGVIVLTDPDFVGEKIRKAISDKVKGVKHAFLSKEEAEAKGDIGVENAAPESILR
                     ALSLAKYELEEVQATFTSEDLTYYGLMGGQNAAELRNRVGSALGIGYSNAKQFINRLN
                     KYGISREELEAALEKVSEQY"
     gene            2662..3519
                     /gene="rsmA"
                     /locus_tag="PKU88_08005"
     CDS             2662..3519
                     /gene="rsmA"
                     /locus_tag="PKU88_08005"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPX69255.1"
                     /translation="MKETSIKTSTLDLMKKYDFKTAKKFGQNFLTDDTVLKAIIDCSE
                     LSKEDCVLEIGPGLGVMTQIFCEKAGRVLAVEIDRKLIQILRVSLFGYENVEIINEDI
                     LKLDLKKVLEEKFGSSPVKIVANLPYYITTPIIMKLLEEDINLRSITIMVQKEVGERI
                     CAAPGGKDYGALTVAVQYRCIPRKILVVPPDSFMPQPEVESMVVRLDFRDKPPVTLKN
                     EKMYFRVVKASFGQRRKTLLNALSAGNLGKTKDELKDILARNGIDEKRRGETLSLEEF
                     ARIANDLLS"
     gene            3599..4888
                     /locus_tag="PKU88_08010"
     CDS             3599..4888
                     /locus_tag="PKU88_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69256.1"
                     /translation="MGSNNEYKRYFIILQEDDKGYELSPGKIPTGYVKIEVKNNKVRL
                     NAFIQNIKLEDRIEYRLILVSSAKKLAVDAGKIIIDGSGRGELSYELEPDNVLKSGLS
                     IGDFNVAAVTCGSSIPLSGYTGRDKLEWKNKYDIVNKPKVQEAPRIDKKIEIEEIVPE
                     VPQPPIHVPLAPQPEPAVVEPAPKPEPAPVPIMEAVPEPEPEPVPEPEPVPEPEVMIQ
                     PAPEPVIIPESKVEVKIVIPECPPIAPMEEPCEIEKVKPIKDKCEYENIEEYYCYDDE
                     EPCKHTASPYYSGGMYSRLKRVLRKLRRYEPFEKERGYSWFKVGDDMYEINRVSVPYM
                     GYMMPLGYPFLSEGCSMMMDRKDYILGVRYEDDDEDDDRRQIKYILFGVPAIYSKRNE
                     MYYKSRGFMEFRPHKTKSYGYFIMCLDIRRGMLCRMN"
     gene            4967..6082
                     /gene="rodA"
                     /locus_tag="PKU88_08015"
     CDS             4967..6082
                     /gene="rodA"
                     /locus_tag="PKU88_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPX69257.1"
                     /translation="MKVENLVDRKLLKNIDLGIVISTILLIAYGMAAISSATHIRSGG
                     SAGTLKVQIIAFILGIIGVIVILLIDYKTFGDNYVLIYIVNVFLLALVLIIGFSTKGT
                     KGWIDLGPVNMQPSEIVKIGYILTFAKYLEKKKDNLNRLIDVLPAMLHLGLIVGLIML
                     QPDAGTALVFIFISIFMLFAAGISYKIIAGAFGAFLISLPVMWKLLKPYQQSRILVFL
                     NPELDPMDKGYHVIQSKTAIGSGQLWGKGLFEGTQNNLGFVLERHTDFIFSVIGEELG
                     LIGALVLMMLFLWLLLRCIHIAKVSKDDYGMLICVGIISMFLFHILENIGMTIGLMPV
                     TGIPLPFISYGGSSLLTNMAAVGLVLNVGMRRQVIRF"
     gene            6399..7643
                     /locus_tag="PKU88_08020"
     CDS             6399..7643
                     /locus_tag="PKU88_08020"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPX69258.1"
                     /translation="MDINKNKVVLAYSGGLDTSIIIPWLKENYDLDVIACCVDVGQRD
                     ELEPIKHKALSTGASKVYIIDAKKEFAEDYIIPTVKAGAVYEGKYLLGTAMARPLIAK
                     KLVDVAEKEGAGYIAHGCTGKGNDQVRFELTIKALNPDIKVIAPWREWTIKSREEAVD
                     YAAARGIPVPVSKERPYSMDRNLWHLSHEGVDLEDTKNPHPTDIYMLTTTPEDAPDKP
                     EYVEIEFNKGIPVKLNGIACDSLELIEELNVIGGRNGIGLVDLVENRLVGMKSRGVYE
                     TPGGTILCSAHKELEYITLDKQTMRFKQMVSDQYADLVYNGLWYTPLKEALDAFVDRT
                     QQNVTGVVKMKLYKGSCVISGMDSIYSLYNKDFATFGENVDNIYNQKDAEGFINLFGL
                     PIEVMALMKAKLAKQQEERKIG"
     gene            7644..9038
                     /gene="argH"
                     /locus_tag="PKU88_08025"
     CDS             7644..9038
                     /gene="argH"
                     /locus_tag="PKU88_08025"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPX69259.1"
                     /translation="MKLWGGRFSKNTALEAEDFNSSISVDSRLYLQDIEGSKAHAIML
                     GKCGIISADEAAEILSGLEEIKQGIESGSIDLKKDAEDIHMNIEKLLIEKIGETGKKL
                     HTARSRNDQVALDIRMYLRDEIHSILNLLTELEALLLDKAEEHAGTIMAGYTHMQKAQ
                     PVTLGHHFMAYFEMFKRDMGRFKDCMKRMDIMPLGSCALAGTTYPIDRKMVAGILKFS
                     DITKNSIDAVSDRDFAIEFISCCAVGMMHLSRFCEELIIWNTGEFGYIEMDDGYSTGS
                     SIMPQKKNPDIAELIRGKTGRVYGDLIALLTIMKGLPLSYNKDMQEDKESVFDAGDTL
                     KSCISVFIPMLEAIIINKERIRNNLIGGFLNATDLADYLVKKGLPFRDAHEAAGEMVK
                     YCIAANKELTELSMDEFRKFSCCIEEEIYEFLKPEVCLSRRNTEGGPSEQRVREAVLR
                     GREYLNHFAETVKF"
     gene            9061..10020
                     /locus_tag="PKU88_08030"
     CDS             9061..10020
                     /locus_tag="PKU88_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69260.1"
                     /translation="MIWVGILGAKGYFGEELSKLIAGHQSAQVSTVLDLQELSESGCG
                     FGRSNGKSAGYLKMMNAVNRSDVIFNGFSGTAAEDIYSKALSNGKKIIDVGDKHIDGS
                     AYPGSVFGLSELYKDKMKDASIAANPSSYCTGAMLGLAPLAAGNLIDINTAVIESKSG
                     ISCLRCGDKLECAGITADDGSKIYKMESRDYAEEVKGQMLALFGKDALSSYVSYIIPG
                     IKGIITSITAEPGTGFCGSDISEIYRGFYKFNPLIEVSGADTINGARNGFDKYFCSIS
                     ASTDADTGKITVTTILDDAVRGSASQAVQTMNLMYGIDGKVGL"
     gene            10194..11849
                     /locus_tag="PKU88_08035"
     CDS             10194..11849
                     /locus_tag="PKU88_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPX69261.1"
                     /translation="MGFNVIIALFAGLSLFLYGMQIMSDALQKSAGDRLKKLLEILTT
                     NKYIGVLVGAAITAVIQSSSATTVMVVGLVNAGIMNLSQAVGVIMGANIGTTMTAQII
                     AFKLTNIVPYAIIIGAILVLFSRKKSRKHLGELIIGFGILFMGMNMMGDAMKPLKDIP
                     EFSQFMIGLEDNPLLGVLSGLLLTAIVQSSSATVGILQVLAMQGLVPIEAALPILFGD
                     NIGTCATALIASIGTGLTAKRAAIMHVTIKIIGTIVFMFILHPVTSLVVMIASEPARQ
                     IANAHTLFNITNTVILLPFSAMLITFVTKVLPGEDVYDKFALKYLDKRILETPSIAVG
                     QIVKEIIRMGEVAKCNVQKGMQAILNNDERVLDEILNNEKVINELERRIGEFLQEVMH
                     SAIGDDQQKMVGMLFNTVHDMERIGDHADNLGEIAQYKLDNKVSFSNYAMEELREIYE
                     NVEEAMDNAFLALSTKDTAYVEAVDAYERKVDELRDSFRESHINRINKGECNINAGVL
                     FLEILTNLERISDHCVNVADVVRIVDRSKLKLDRVSEISPEKC"
     gene            11947..12627
                     /locus_tag="PKU88_08040"
     CDS             11947..12627
                     /locus_tag="PKU88_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPX69262.1"
                     /translation="MNNHDYLRQISIFSELEGEFLEKIYQISRVRKYKKGRIIFMEGE
                     PGEAFFYIKSGLVKISKLSRDGREHILHVLNEGHVFAEVTLFSNTEYPATAEVLEDAE
                     IGIIKNEDLEKVIKENPDLSLQLIKYLNKRLVEAHMKIRNLALYDTYERTAQALVKLA
                     EDYGKKSSKGVNLDINISRQELANIVGTTRETVIRALTAFKKEHLIGIEKNTITIIDL
                     ESLKEWPY"
     gene            13025..13228
                     /locus_tag="PKU88_08045"
     CDS             13025..13228
                     /locus_tag="PKU88_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69263.1"
                     /translation="MNNIIHQICENYISEVLHFFDSGAIRKLDEIEVVLKEKTNSYLK
                     QMTQTYFEEIDQAIADDKTGGRS"
     gene            13710..16709
                     /locus_tag="PKU88_08050"
     CDS             13710..16709
                     /locus_tag="PKU88_08050"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HPX69264.1"
                     /translation="MEGYKLRYKLITFLSSGNIKDISSVYAKTFDIALFIFPSRRSFI
                     PFDRIEAIISETLEVYSEKNLVQVPPFDNLEPTITNLGNVFFVLLKEALFQKDLVLET
                     LEISESPIRSYIVNEVNADRGFLVGHKKIKATSLLMDDIISQSTYHLLSENEKEGQTP
                     IPVVHAPAPVAEAGLPLPPPKPKIEDSSPTLQPVKVPVYQFTLCFLLLTVCGVLITIY
                     LKKTGAYPSGSDIYGHLFKSDFLYHSIRAGDFYPLYTDLWYNGMQPFRYWAPLPYYFL
                     VILQFIAGGDAISSYLLFVLFAFVIGGTGWLLWGWAYNRMLFTTFLGVLWFFLPDNIR
                     VFFVEGNFPRMAIAIFLPYLFYFVWRYVEHSKTWAVVPVIFIMCCIILSHVMIAAMTG
                     IGTFIFLFIYSMRQKRLLESFQIIVAMLLSFALCGVWLYPALQGGLMGMNAAATSEVM
                     QSLSTPISISLNPLLRTQEIFDYYYFGLSILVISIIGIFVADKKSLPGFLTSIIIFLG
                     TTTVVVPFLEKLPLNQLLWMMRFTPIVYAIFMLALLEWRNCRRYVAIIIAFFLIIDSI
                     PSAHLEKYFSHNPARLSDSLSTTKELTRQRVSLIDLSVFDSYPSYYFSSKEPKTKYTF
                     GWAWQGAATANNIMMINTAAEKGYYYYLFDRSLELGDDTVMVHKELVDKAKRTMTSLL
                     EAAQASGYKFYKETNFTYIFHRDTPACFGVASEYAGLAIGNSAKAIALEYPSFEEGSS
                     DNLTDYRYEDLSRYKVIYLSGFSYNDKKAAEELLTRTANGGVRIIIDMGRIPVDPVTS
                     RMTLFGVTAQAISFSAQYPELMYHDQVYQALPFKEEYSTWNTFYLDNVEQAIGYSWFK
                     NKNMPFLGTAGSPNIIFMGYNILFHAMETQDESIIMLINDLLEFEPTQLPDREVVSIS
                     VKKQKDKLIIDSEKGSINTTIAYQDNFRSDQKIYEKNNLLVVQEPHTEIEITYAYLAQ
                     GLALSGAGVLGTGVLLYFIIRKRRCVQ"
     gene            16706..17338
                     /locus_tag="PKU88_08055"
     CDS             16706..17338
                     /locus_tag="PKU88_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Firmicu-CTERM sorting domain-containing protein"
                     /protein_id="HPX69265.1"
                     /translation="MKKIIQKVLIGLLLIQAVSFSGYADYGSIDIDGYYDDWEDKPHT
                     EVYYGKKPYVSEIHLVSVFRDETTVYVHIKMSENGYTELPNSYFTMDTDMGSEAFTLM
                     LDSMKKKESGTAGIEVHLTDNWDVVGSGYYTREEGESDEAEFEIPLYSITDEPDGVVD
                     IAIMFPDLGSQDIVCVGAGTGPYIAVAIGAVIVLLSLAYYYKKKHPAYAK"
     gene            17351..17953
                     /gene="xrtG"
                     /locus_tag="PKU88_08060"
     CDS             17351..17953
                     /gene="xrtG"
                     /locus_tag="PKU88_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase family protein XrtG"
                     /protein_id="HPX69266.1"
                     /translation="MLTLKIIAFIVWIYVIWVLSRTGLHFFKFCIGSVGLFFFLMLWI
                     QPLIMQPLKMAVSAAAGVLGDFFEMYDSYYQYSILFIPKDTTAVSLTIDYECSGVIEI
                     MAFSCMLWFFPLYNTIEKLIINCIGLAVIFLANVLRIFLICTLIYFYGNEIFYFAHTI
                     FGRIFFYSISIALYFYVFTRSHIIRQKVGSFNYGNGDTDK"
     gene            17928..19301
                     /locus_tag="PKU88_08065"
     CDS             17928..19301
                     /locus_tag="PKU88_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase, exosortase G
                     system-associated"
                     /protein_id="HPX69267.1"
                     /translation="MEMEILINRIIREVIFWMAWIIIPLIMEIIPAFGGFFILLKKSF
                     VLKGKAIPQAVSEITLIIPIYNSAETLRTCLTSVHNSNYPIELMDILLVNNGSTDNSY
                     EVYRQCQDEFRDLPMTWLNASQGKSKALNMALYNSHGKYIVHIDSDGKLHPDALRNMA
                     IRFEQRPEVDCLTGVILTDMKLIESTNSFMMKLVRRCEFFEYCQSFLAGRNFESEFNS
                     IYTLSGAFSAFRKSAILKSQLFNTATVSEDTQVTFQMHKIQKKGISLCENAFFYVDPI
                     ESYNKLYTQRQRWQRGEIEVSHMFLQEELTIIKGFISNFMVRTLVFDHTFAFPRMIWY
                     FALIFLVFLNYPIALVLSSIGIIYLLYSFSMFLYYINVLMYLDKVKDIQRYYRSKWYL
                     IFVLPGFNFVVFWIRFAGIINSIKGEGVWKTTSLTEEWNKIKVAVNDDFHFPKRVIAA
                     LRRKVNL"
     gene            19298..20077
                     /locus_tag="PKU88_08070"
     CDS             19298..20077
                     /locus_tag="PKU88_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX69268.1"
                     /translation="MNTLTVALPAYNEEENIEALVSSWQQYREILMDKYGLILQIVVI
                     NDGSKDKTVEIAEDLKRKYSNFTLVNHIHNKGLGEAVKTGIAYFLDNCPESRYMCLMD
                     CDNTQDPCYITDMLDCSNRTGADVIIASRYRKGSEVMGVSGFRLIMSGGARYVFSVLL
                     NIPGVRDYTCGYRLYSREILRKASYRFEKDLIEESGFTCMAELLYKLFTCGAVFGEIP
                     FVLRYDYKKGTSKMSVLKTAINSMGLALRLRKIKMTEKSDE"
     gene            20070..22709
                     /locus_tag="PKU88_08075"
     CDS             20070..22709
                     /locus_tag="PKU88_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HPX69269.1"
                     /translation="MSEITENKITDSKLQYRIELFSALIITGIVCAAGSSNLLFASND
                     LYPAATDAMGHMAKVQYITQCLKGGVIPSWFPYWYNGAAVTQYYPPLSYYLMVPVYAL
                     TGNVMLTFKINCFLMMFIGGMGVWYFCRKVIGKWCGCIAIVFYCLQPFLLSSLYIVGV
                     LAQGPVYAITPWLLYLVLSFIKEPNRQKALFCTIATALLILSHPMHAFIIVLCIMLVL
                     LMFIPSKRISLLNYTVLVVMMTFSLIITAFWSLVGVTGLENPTIPYILPGVAANVTAT
                     LKWFQPGNMGGLYFYFALPILVFTVIAAMALILRTTKKQVINAERFEIPFCITLTFFT
                     TMFSFGRQIPGFKLLPMTDSLVSGRILSLTALTGAIACAYLTFTVIGYIPTLRRMMRL
                     VILAIALSAFVLTIVHINPYKFSFPIGSYEKFEKRFESVAGDISVSPFDKGRYIWFSG
                     LSSDEANIPIAYGFTISEGWNMEGSPHNRALWNHLLCIATDTRDYLAKNFAFWNVRYF
                     MLPTQYDEMVIPLKEHLGFRLAYDTEFYTSQLPSSYFLTDNRNGLIFGIGAPGVAMEF
                     PYLVYEQRRDISDYSLAELSEYKLIYLCEPVVDTMRQKERIEETIKYLIDKGVRIIIE
                     PTPTKGFKLFDVAVSDVPAEDSLLLEKQYMSPLNSIIDNVKIDEKHGYFRALFGLDEA
                     YYKSVQNGGLLQNDIIGTKRVGKGEVLFLGMHLSQYLKAVYALNLGVPEDIKGYPSCS
                     DEIKALFGDIFKTYGVEQNFWPEPFPVNNVRWNYKGVDFDYSSQAAQEMTISITYTPR
                     WKATIDGKPVDVGQRENLITLQLPAGEHKVQLKYGLTKYAVIGYTTLIAGLLLFSLFL
                     RNYDINVINLRKLCALLKNYLQV"
     gene            22838..24532
                     /locus_tag="PKU88_08080"
     CDS             22838..24532
                     /locus_tag="PKU88_08080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPX69270.1"
                     /translation="MFQELNKYKDSIIKNYSSTQESLVTEVSKKVKESLLTGSGEEGV
                     IDLLSNAGTSGSRYWFLYTASGAVFEKDYEETRKIKGKSASELIKFWKLQGGTGTEDF
                     EKLLLQKKNGSSVFTKSADYGEEIVSVKYFIANNTGYFLGMSTQHQYVLYVGRVNEHI
                     LYLYIFAGAVSLALLVLALFLCLTLYKGQKRCDKANKIIKDNNFHIEDLTKKLASKTD
                     TVKKASIFDDLTKHYNRKFFYNLLSKINDEHFQPISIVVLDINGMDRLNRTAYYRTGD
                     EVLEKTSGILQRLCLDTDIVARTGGAEFSVLMTVTNISEAYGITENIRRQFTGLNHAD
                     LTLSIGVAQMQPEDNSIFSALDRARKNLILEKLNDKNSSDYSIIAMLMEILGAYSLST
                     VAHCNRLREKALIIGKSLRLSNSELSRLAIAAQLHDVGKIGISDTILNKKEMLTRHEK
                     DCIYRHPDIGYEIIKLIPPLDEVAIDIRQHHEFYDGTGYPAGLKGEEISLCARIINVI
                     DSFDAMTNKSVYAKVKTPEEAIEELQKKSSIQFDPHIVDVFVKVFSDNIKVGSEIA"
     gene            24963..25475
                     /locus_tag="PKU88_08085"
     CDS             24963..25475
                     /locus_tag="PKU88_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPX69271.1"
                     /translation="MTYSQYRFKFYFNASHAIYLMGQLGESHPHTWELIIHGAKITDD
                     FAPFNEIEKKIEDFLSQYQDVFINTVQPFTTINPTLENICTHFKTSIQELLYLKGWLL
                     LSIELSETPTRAYVISMHDEAESKKSYYEKAGAETYQDVVDELVFEKLDSLFENREIA
                     AEKDTDGEQK"
     gene            25773..28253
                     /locus_tag="PKU88_08090"
     CDS             25773..28253
                     /locus_tag="PKU88_08090"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPX69272.1"
                     /translation="MKRIRGSRFLIKALVILLIASITFPGAVYSLESAKLIEDGQHIG
                     PGTYYRNMNYITPNGNFMVNMIESKFDDKYISVEVADSGSSLTNKPVSYQASLKNDEN
                     KRVIGAVNGDFFDMTTIKGLTYGTSIIEGEIKTAVKTSTILGVTDKGDCFIDTLNMEG
                     AAAYKYIQYPVDSVNRLRWADQTVIYTPFFGKTTVNTVAGTDIIVRGVELPLKAGKRY
                     TGKIEKIIPDSKITEIPSDGVVISMPVKAFRQLAGVPSGAEMSFYVDFDKKNLDFAVS
                     GAPRLLEGGQISAKVKERADALQRHPRTAIGIKDNKIYMVTVDGRQLGYSDGMTLYEL
                     VEFFLSQGVENAINLDGGGSTAMAVRKQGDAAAKLANNPSDGRERYVGNSIQLVSEAP
                     LSDPAILRFKDTSVKIYKNSSFKPSYYVLDKYYNLLPGDMGKVRFGIDVKTSKIAADG
                     TYSSGSQAGKGYIYAACGDTQSKMLVEIVDKVSSLAVTNDFVLLDPGEKTQLQVKAFD
                     ENGGNIIISPSAVKWTVEGELGTVDKDGVFTAGNNNGHGKITASAGTVTAAVGAKIGK
                     TPVIVADFGTLDNVEAKFIRSKAAIRHNQKGEPVRIGNISLRLDYDFQNTEGTSAAYV
                     TFKKPVTIIGKPIELGVWVYGDGSSHWIRGNYINSAGEKKVFDLTKSGGLDWKGWKYV
                     YAELPKDEKYPIALEQIYLAETAQDNKNSGSIYFDDVMAIYKLDKDYYDPVIVSVLSE
                     SSEGLPGQAQEIGVFAADRGYGIDPASIKMYINDSAVKASFEPETGKISYIPAKPLKK
                     GEYKVRISLKDKIGHSLNPEYAYTFTVK"
     gene            complement(28339..28995)
                     /gene="trmB"
                     /locus_tag="PKU88_08095"
     CDS             complement(28339..28995)
                     /gene="trmB"
                     /locus_tag="PKU88_08095"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPX69273.1"
                     /translation="MRIRNNPKALEIIKQYDEIVIFRPGEYKGNWNEYFSRRTGNKED
                     APIRAELGMGRGRFISTNGLLNRDINFIGIDLRHEIILSALSKVLEMELQNVILIPYN
                     ILDIEDAFEPSEVDTFYINFCDPWPKVRHAKRRLTHRLFLGKYRTLLKNGGRIEFKTD
                     SRELFDFSINEFKASGFEILDLTYDLHNEDYPGNITTEYEEKFIKKGIKINRLEAVKK
                     "
     gene            29202..>30321
                     /locus_tag="PKU88_08100"
     CDS             29202..>30321
                     /locus_tag="PKU88_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPX69274.1"
                     /translation="MQLSTKILIGLAAGILAGVLLQGSPEIATTWIKPFGTLFLNLIK
                     MIIVPLVLASLVVGTCGLGDVKKLGRIGGKTMAYYLLTTAFAVILGLILANMFSVGAG
                     FVLPSDVKAAEASKMPSVIDTLLNIIPTNPIKAASDGNMLQIIAFALILGSGIVVLGD
                     KGKPVYEFFDSLAEISYKITGGIMALAPYAVFALITPVVASNGPKVLLPLLSVIIIVY
                     VGCALHAALVYSGTLMLFAKFSPMKFFKGIFPAQAIAFTTCSSSATLPVNMQCTEENL
                     GVPKSICSFVLPLGATINMDGTALYQGVCALFIANVYGLDLTMAQQATIVLTATLASI
                     GTAGVPGAGLIMLTMVLQTVGLPLEGLALVAGIDRILDM"
BASE COUNT         9380 a         5239 c         7031 g         8671 t
ORIGIN      
        1 aaacctcgaa cctcgcgcct cgtacctcgt acccgaagaa tgcgtcgcat tcttcctata
       61 ttgcctaaat tcccctcata tagtaaaatt agcatatata gcaaattaag aagctaattt
      121 gcataaacat atgtaatgtt tttcatatcg agcttatttt tttattagca atattccaaa
      181 gcattatcaa accttccggg ggtactgcaa tgattactta tgaaatcaac gatgcccttt
      241 atataaacct taccaacagg tgtacaaaca actgcagttt ttgtatcaga aatcatcccg
      301 aaggttttgg atttgatctt tggcttccga aggaacccgt tcttgaggaa ataatggcgg
      361 acctggagaa cctggagctt tataatgaag tggtgttctg cggttatgga gagccattgg
      421 tacgatacag ggaaataatc gggatatgca ggcatataaa gcaaaaggga gtcagaacaa
      481 ggataaatac caacgggcag gcaaatttaa tatggaacag gaacattgta cctgagctta
      541 aaggactggt ggattcaata tctataagcc tgaatgcaaa aaatgcggag gactatgcgg
      601 agctttgccg gccggaatac ggaaaggcag cttacagcgc agtattggat tttgcgggag
      661 aatgtataaa atatattccg gaagtatggc ttactgttgt tgacgtgatt tcagcctacg
      721 atattgaggt atgcagaggc atagcagaag gcattggcgc aggcttcaga gtcagacact
      781 attgttaggt gctgttttgg tgaataaaat gctttattat ggcaaatata tatatgaatg
      841 ataatattcc ccaatttacc agagattgta tagatgcttc gtggaaaagg taaatttgac
      901 aatggaagca aatacaggta aaataaaaat acttgcctga cgattgaggc agaattggca
      961 agtaaaacgg atgctaaaag tgaaatcaag acaggcattc ggttaaacaa aaaggaggtt
     1021 gaacgatgaa aataaacttg aaggatttca agagtttatt ttcaatcaag cttattgctg
     1081 tcgctgcaat atgtttgata ttcgctgcat cttatgttta cagtgcgatg aaaaaagaaa
     1141 ttactattga agatattgga ggagtaaaag ttgtaagtac ctttaaggaa accgtatccg
     1201 atgtgctgga agaaaataat atacaactga taccacagga taagataaac ctgggtatga
     1261 cggaaaagct tgaagacgga acgcacataa aaatatccag ggcaaagaag gttacaatta
     1321 gttcagatgg tgaggatatt gttatcctga ctactgctcc tgatgtgaaa agtgcattag
     1381 gggaagttca gataaagctg ggggagaaag acagggtttc acccggtttt gatactgcaa
     1441 ttacgggaga tacaacaatt gcagtaacaa gagtggaaga gagaattgta aagcaagaga
     1501 tgaaaatgga ctttaccaat gaagtgagaa aaagcgacaa ccttgacaag ggcttggcac
     1561 gggttataaa aaagggcagc caagggtcaa aagaactgtc aataaaggtt atattcgaga
     1621 atggtaagga agttaaacgg gaaatcgcag aagaaaaggt gcttaaagag cctgtgaacg
     1681 gcattattga ggaaggaacc agaactacct ttgtatcttc aagagggcag gttaccagat
     1741 ttgtaagagc tctaaagatg actgcaacag cttatgatgc cacctttgaa agctgcggca
     1801 agcatccgga tcaccctcag tacggtatta cctattcggg tttaagagtt aggcccggta
     1861 tcgttgccgt tgaccccaag gtgattcctc tggggacata tctttatgtt gaagggtatg
     1921 gggaagccct tgcagctgac ataggcagtg ctataaaagg aaacaagatt gacctgtatt
     1981 atgaatcccc ggaagatgta gcgaaatatg gaaaaagaac tgtaaaggta tatgtgcttg
     2041 ataagcccaa atacaaattc taaaaaatgc gtcgagagac gcatttttat tgcgaggtat
     2101 atcaatgtta aaggaaacaa ttgtggtgga aggcaagaac gatgctgctg cagttaggcg
     2161 ggcagtggaa gcggatataa taattaccag cggttttggc ataaacggaa gtattctgga
     2221 aaggataagg actgctcagg ataggaacgg ggtgattgtt cttaccgatc ccgattttgt
     2281 gggagagaag atacgaaagg ccatttccga taaggtcaag ggagtaaagc atgctttcct
     2341 gtctaaggaa gaggctgaag caaaaggaga tatcggggtg gagaatgcag ctcccgaaag
     2401 tattttaaga gccctgtctt tggctaaata cgaactggaa gaggtccaag cgacatttac
     2461 aagtgaagat ttgacatatt acggccttat gggagggcaa aacgcagccg aactgaggaa
     2521 cagggtaggt tccgcattag gtataggcta ttcaaacgca aagcagttca taaaccgttt
     2581 gaataagtat ggtattagca gagaggaact tgaagcagct ttggaaaagg tatcggagca
     2641 atactgaaaa ggatgataga tatgaaggaa acatcaatta aaacgtcaac tttggattta
     2701 atgaagaagt atgattttaa gacggcaaaa aaattcggac agaatttttt gacggatgac
     2761 actgttttaa aggcaatcat agactgctca gagctttcaa aggaggattg cgttcttgaa
     2821 ataggccccg gactgggagt gatgacacag atattctgtg agaaagcagg cagggtactg
     2881 gcggtggaga tagacaggaa gctcatacag atacttaggg tttcgctgtt cggttatgaa
     2941 aatgtggaga taatcaatga agatatttta aagctggatt tgaaaaaggt gcttgaagaa
     3001 aagttcggca gttctcctgt aaagatagtg gcaaatttgc cttattatat tacaactcct
     3061 ataataatga agttgctgga ggaggatatt aacctcagga gcatcactat tatggttcag
     3121 aaggaggtag gggagaggat ttgcgccgcc cccgggggaa aggattatgg tgcccttaca
     3181 gtggcagtac agtaccgatg cataccccgc aagattcttg ttgtccctcc tgattcattc
     3241 atgccgcagc ccgaggttga atccatggta gtgaggctgg acttcaggga taagcctccc
     3301 gtaactttga aaaatgaaaa aatgtatttc agagtagtga aggcttcctt cggtcagcga
     3361 aggaagaccc tgttgaatgc tttgtccgca gggaatctgg gaaaaacaaa agatgagctt
     3421 aaggatatat tagcaagaaa cggcattgat gaaaaaagac gaggtgagac tttgtctctg
     3481 gaggaatttg cgagaattgc caacgacctt ttatcctgat aaaacagaaa aaatttgttc
     3541 acttttaacc ccctcataca tatattagta atgactatgt atagaagggg gttattatgt
     3601 gggatcaaat aatgaatata aaagatattt tataatactt caggaagatg acaaaggata
     3661 tgaattgtca cccgggaaaa taccgacagg ttatgtaaaa attgaagtga agaataataa
     3721 ggtaagactc aatgcgttta ttcagaatat taagcttgaa gatcggattg agtatagatt
     3781 gattcttgtg tcatcggcca agaagctggc agtggatgca ggaaagataa ttattgatgg
     3841 gagcgggaga ggagagcttt cctatgaact tgaaccggat aatgttttga aaagcggttt
     3901 gagtattgga gatttcaatg ttgccgctgt gacctgcgga tcatccatac cgctttccgg
     3961 ttataccggg agggataagc tggagtggaa gaataaatat gatatagtaa acaagcctaa
     4021 ggttcaagag gcaccaagga tagataagaa gatagagatc gaagaaatag ttccggaagt
     4081 accgcagccg ccgatacatg ttccgcttgc cccacagcct gaaccggctg ttgttgaacc
     4141 tgccccgaag cctgagccgg cacctgtccc cattatggaa gctgttcccg aacccgagcc
     4201 ggagccggta cccgagcctg aacctgtgcc tgaaccggaa gtcatgatac agcctgctcc
     4261 tgagccggta attatacctg aaagcaaagt ggaggttaaa atagtaatac ctgaatgtcc
     4321 tccaattgct cctatggaag aaccctgcga aatagaaaag gtgaaaccga taaaagataa
     4381 gtgcgaatat gagaatatag aagaatacta ttgttatgat gatgaagagc cctgcaagca
     4441 cactgcatct ccttattaca gcggaggaat gtacagcagg cttaagagag tactgagaaa
     4501 gctgagaaga tatgaaccct ttgaaaaaga gagaggctac agttggttca aggtaggaga
     4561 tgacatgtat gagataaaca gggtatcggt tccatacatg ggttatatga tgcctttggg
     4621 gtatccattt ttgtctgaag gctgcagcat gatgatggac aggaaggatt atatactggg
     4681 tgttagatat gaagacgatg atgaggatga tgacagaagg cagataaagt atattctctt
     4741 tggagtgcct gcgatatata gcaaaagaaa tgaaatgtat tacaaatcaa gaggattcat
     4801 ggaattcagg cctcataaaa caaaaagcta cggatatttc ataatgtgtc tggatattag
     4861 gcggggcatg ctgtgccgta tgaattaaag caaaaaaata aaacaaagtt gaatttttta
     4921 tgttagaata atattaagta ttgaaataca gaagaggaat gattttgtga aagttgagaa
     4981 tcttgtggac aggaagctcc ttaagaatat agatttgggc atagtaatat ccactatact
     5041 gcttattgca tacggcatgg cagcaatttc aagtgctact catattagga gcggaggctc
     5101 tgcgggcaca cttaaggtac agattattgc ttttattctg ggaataatcg gagtaatcgt
     5161 gattctgctc attgattaca agaccttcgg ggataattat gttcttatct atatagtcaa
     5221 tgtctttctt ttagccctgg tattgattat tggcttttcc accaagggaa ccaagggctg
     5281 gattgacttg ggcccggtga acatgcagcc ttctgagata gtaaagatag gatatatatt
     5341 gacctttgcc aagtacctgg aaaaaaagaa ggataacctg aacaggctga ttgatgtatt
     5401 acctgcaatg cttcatttgg gactgatagt aggcttgata atgctgcagc cggatgccgg
     5461 caccgcattg gtatttattt ttatttcaat ttttatgctg tttgctgcag gtattagcta
     5521 caaaataata gcaggcgcct ttggtgcctt tttaataagc cttccggtaa tgtggaagct
     5581 tttgaagccc tatcaacaga gcagaatact tgtgtttctg aatcctgaat tggatcccat
     5641 ggataaaggc tatcatgtaa tacagtcgaa aacagctatc ggatcgggac aattatgggg
     5701 caaggggctt tttgaaggta ctcagaacaa tcttggcttc gtacttgaga ggcatacgga
     5761 ttttatcttt tctgttatcg gggaggaact gggactgatt ggtgctctgg ttttgatgat
     5821 gctgttcctg tggctgcttt tgagatgcat acatattgca aaagtatcaa aggatgatta
     5881 tggaatgctt atatgcgtag gaataatatc aatgtttctc tttcatatat tggagaacat
     5941 aggaatgacc atagggttaa tgcctgtaac aggtataccg ctgcctttca taagctatgg
     6001 gggaagctcc ctgctgacta atatggctgc ggtaggactg gtgcttaatg tgggtatgag
     6061 aagacaggta ataaggtttt aaaagagtat aaccaaaggg gtgccgcaaa actgcggcat
     6121 ccttttttca tataataaga aaattgcgac ggggacacgg ggacgcgggg acacggggac
     6181 gtttcccctg tcccgctttc agtgggacac gcgaaacgtc cccgtgtccc cgcgtccccg
     6241 cgtcctcatg tcgcattatt cttattatat gaatataaat tcatagagga aaaaaattta
     6301 ataaatattg taataagtac ttgcatattt atgcatacga tattataatt ataattatta
     6361 ctattattat tactaataca atagggggag tctcaaatat ggatattaat aagaataaag
     6421 tagttcttgc atattccgga gggttggata cctccataat aattccctgg ttaaaggaaa
     6481 attacgattt ggacgtaatt gcatgctgcg ttgatgtagg gcagagggat gaactggagc
     6541 cgataaagca taaggctctg agcacgggag cttcgaaggt gtatataata gacgcaaaaa
     6601 aggaatttgc agaggattac attataccaa ctgtaaaagc cggagctgta tatgagggta
     6661 aatatcttct ggggacagca atggcaaggc cgctgattgc aaagaagctg gtagatgttg
     6721 cagagaagga aggagcagga tatattgctc acggatgcac cggtaaagga aatgatcagg
     6781 tacgtttcga actgacaata aaagctttga atccggatat aaaagtcatt gctccatggc
     6841 gtgagtggac tataaaatcc agagaagaag cagtggatta tgcggcagca agaggaattc
     6901 ccgttcctgt ttccaaagag cgcccataca gtatggatag gaacctttgg catttaagcc
     6961 atgagggagt ggatttggaa gataccaaaa atcctcatcc tacggatatt tatatgctga
     7021 caacgacacc tgaggatgct ccagacaaac cggagtatgt cgaaatcgaa ttcaataagg
     7081 gcatacctgt gaagcttaac ggtattgcct gtgattcttt ggaattaata gaagagctta
     7141 atgtaatagg cggcagaaac ggtataggcc tggtagattt agttgagaac agacttgtag
     7201 gaatgaagtc aaggggagta tatgaaactc ccggcggaac catactttgc tccgcccata
     7261 aagagctgga atatataact ctcgacaagc agacaatgcg attcaagcaa atggtttccg
     7321 accagtatgc agatcttgta tataacggct tatggtatac tcctttaaag gaggccttgg
     7381 atgcttttgt tgatcgtact cagcaaaatg taacaggtgt tgtgaaaatg aagctttata
     7441 agggttcctg tgtaatttcc ggaatggact caatttattc tttgtacaac aaggattttg
     7501 cgaccttcgg cgagaatgta gacaatattt ataatcaaaa agatgctgaa ggctttatca
     7561 acttgttcgg cctgcccata gaggtaatgg cgcttatgaa agcaaaactg gccaagcagc
     7621 aggaggaaag gaagataggt taaatgaaac tctggggagg aaggttcagt aagaataccg
     7681 cacttgaggc tgaggatttc aactcttcga tctctgttga ttcgaggctg tacctgcagg
     7741 atatcgaggg gagcaaagcc catgcgatta tgctaggaaa atgcggaata atcagtgccg
     7801 atgaagcagc agaaatacta agcgggctgg aggaaatcaa acaggggatt gaatccggaa
     7861 gcatagacct gaaaaaggat gctgaagata ttcacatgaa cattgaaaag cttctaatcg
     7921 aaaaaatagg tgagacaggt aagaagcttc atactgcaag aagcagaaat gatcaggtgg
     7981 ctttggatat caggatgtat ctcagagatg aaatccatag tatactgaac ctgctgacgg
     8041 agcttgaggc cttgctgctt gacaaggcgg aagaacatgc cggaaccata atggcgggat
     8101 atacccatat gcagaaagca cagcctgtaa ccctgggaca tcattttatg gcttactttg
     8161 aaatgttcaa acgggatatg ggcagattta aagactgcat gaagaggatg gatataatgc
     8221 ctttagggag ttgtgccctt gcagggacta catatcccat agacagaaaa atggttgcgg
     8281 gaatcctaaa attttcggat attaccaaga attccataga cgcggtatca gacagggact
     8341 ttgcaattga gtttatcagc tgttgtgctg ttggaatgat gcatctcagt cggttctgcg
     8401 aggagttgat tatatggaat accggagaat tcggatatat tgagatggat gacggctaca
     8461 gcacaggaag cagcattatg cctcaaaaaa agaatcctga tattgcagaa cttataagag
     8521 gcaagaccgg cagggtatat ggagacctga ttgctcttct taccattatg aaggggcttc
     8581 cgctatccta caacaaggat atgcaggaag acaaggaatc ggttttcgac gcgggggaca
     8641 ctttaaagtc ctgcatttca gtatttattc caatgcttga agctattata ataaacaaag
     8701 aaagaataag gaacaatctt ataggcggat ttctgaatgc caccgatctg gctgattacc
     8761 tggttaaaaa aggattgccc ttcagggatg ctcatgaggc ggcgggggaa atggtgaagt
     8821 actgtattgc cgccaataag gagctgacag agctttccat ggacgaattc aggaaatttt
     8881 cctgctgtat tgaagaagag atatatgagt ttttgaagcc ggaagtatgt ttgtcaagaa
     8941 gaaacactga aggagggcct tccgaacaga gagtcagaga ggctgtattg cgcggaagag
     9001 agtatctgaa tcattttgcg gaaacagtta agttttagca tatattgggg gaaggtattt
     9061 atgatttggg ttggaatttt aggggcaaag ggatattttg gagaagagct ttcaaagctg
     9121 attgccggac atcagtcggc gcaggtgagc actgtcttgg acttgcagga gctgagtgaa
     9181 tccggatgcg gatttggcag aagcaatgga aaaagtgcgg gatacctgaa gatgatgaat
     9241 gcagttaata ggtcggacgt tatatttaac ggattttccg gaactgcagc agaggatata
     9301 tactcgaaag cactttccaa cggaaaaaaa ataatagatg tcggtgataa gcatatagac
     9361 ggcagtgcat atcccggctc agtatttggg ctgtctgagc tttacaagga taagatgaag
     9421 gatgcatcca tagctgcaaa tccaagcagt tattgcaccg gcgcaatgct gggattggcg
     9481 cctctggctg caggcaatct gattgatata aatactgcgg ttattgaatc caagtcgggg
     9541 atttcctgcc tcagatgcgg agataagctt gaatgtgcag gtatcaccgc tgacgacggc
     9601 agcaagatat acaaaatgga gagcagggac tatgctgaag aagttaaggg gcagatgctt
     9661 gcgctgtttg gtaaggacgc cttatcttca tatgtatcat atataattcc gggaatcaaa
     9721 gggataatta catctataac ggccgaaccc ggtaccggat tctgcggaag cgatatttca
     9781 gagatttaca gaggttttta caaatttaac cctttaattg aagttagtgg tgctgataca
     9841 ataaatgggg caaggaacgg attcgacaaa tatttttgct ccatatcagc ctcgacagat
     9901 gcagataccg gtaagataac agtgactaca attcttgatg atgctgtaag aggatcagca
     9961 agccaagcgg tacagacaat gaaccttatg tatggtatcg acggaaaagt aggtttatag
    10021 ataacaaagc aagccggcat cggcttgctt tgttttttca cagcatagca gaataataaa
    10081 ttgtacaggc ttttaataag cttgttcttt ttgttttatg atacttgatg tcgaaaggcc
    10141 aaacaggtat aattaattgg gtactgcatt ttattagcca tggaggtgga gttttgggtt
    10201 ttaatgtgat tattgcacta tttgccggtt taagtctttt cctttatggt atgcagataa
    10261 tgtcggatgc tcttcagaaa tctgccggag acagattgaa aaagctgctg gagattctta
    10321 caacgaacaa gtatattggg gtcctggtag gtgcggcaat tacagcagtt attcaaagca
    10381 gcagcgccac cacagtaatg gtggttggcc tggtcaatgc agggataatg aacctttcgc
    10441 aggcagtagg ggtaataatg ggggcgaata taggcactac aatgaccgct cagataattg
    10501 cctttaagct taccaacata gtcccctatg ccattattat cggtgcaata cttgtgttat
    10561 tctcaaggaa aaaatcacgt aagcacctgg gtgaattaat tatagggttt ggtatactgt
    10621 tcatgggtat gaatatgatg ggtgacgcca tgaagccttt gaaagacata cctgaattct
    10681 cacaattcat gataggcctg gaggataacc cgcttctggg ggttctttcc gggttgctgt
    10741 taactgcaat agtccaaagc agcagcgcaa ctgtcggtat acttcaggtt ctggctatgc
    10801 aggggctggt tcctatcgaa gcagcactgc cgatattatt tggtgacaat atcggtacat
    10861 gcgctaccgc cctaattgcc agcataggta caggcctgac ggctaaaaga gcagctataa
    10921 tgcatgtgac aattaagatt ataggtacga tagtatttat gtttatactg cacccggtaa
    10981 cttcattagt ggttatgatt gcttcagagc ctgcaaggca aatagcaaat gctcataccc
    11041 tatttaatat tacaaatacc gttattctac tgcccttctc cgcaatgctt attaccttcg
    11101 tgactaaagt actgccggga gaagatgtgt atgataaatt cgcattaaag tatctggaca
    11161 agagaatact ggagactccc tcaatagctg tcggacaaat tgtaaaagag attatcagaa
    11221 tgggagaagt tgcaaagtgc aatgtacaaa aaggaatgca ggcaatttta aataatgatg
    11281 agagagtctt agatgaaata ctgaacaatg agaaagttat aaatgaattg gaaagaagaa
    11341 taggtgagtt tcttcaggag gtaatgcatt ccgcaatagg ggatgaccag caaaaaatgg
    11401 tagggatgct gttcaataca gtacatgata tggagagaat aggggatcat gcggataacc
    11461 ttggagaaat agcccaatat aagctggata ataaagtttc cttctccaat tatgcaatgg
    11521 aggaattaag agaaatatat gaaaatgttg aggaggcaat ggacaatgca tttttggcct
    11581 taagcaccaa ggatactgct tatgtcgaag cagtagacgc ttatgagaga aaggttgacg
    11641 agcttagaga ttcatttagg gaaagccata tcaacaggat aaacaaaggg gaatgcaata
    11701 taaatgcagg ggtgcttttc ctggagatac tgaccaatct tgaaaggatc agcgaccact
    11761 gtgttaacgt tgcggatgtt gtcaggattg tagacaggtc aaagctaaag cttgacaggg
    11821 tgagtgaaat atctcccgag aagtgctgaa acaacggcag tcatgtgtaa gctgtaaaag
    11881 cttgagcatg actgctattt ttatataata taggtatata acgaggtgaa agggagaaga
    11941 atttgtatga ataatcatga ttatctgagg cagataagca tcttttcaga gcttgagggt
    12001 gaatttcttg agaaaatcta tcagatatca agagtccgta aatataaaaa gggcagaata
    12061 atattcatgg agggagaacc gggggaggcc tttttctata taaagtccgg ccttgtcaag
    12121 atctcgaagc tttcaagaga cgggcgggag catatacttc atgtattaaa tgaagggcat
    12181 gtttttgcag aggtgactct tttcagcaat acggaatatc ctgctacggc agaggttctt
    12241 gaagatgcag aaataggaat aataaagaac gaggatttgg aaaaagtcat aaaggaaaat
    12301 ccggacctgt ccttgcagct gatcaagtac ctgaacaaaa gacttgtaga ggcacatatg
    12361 aagattagga atctggcgct ttacgataca tatgaaagga ctgcccaggc tttggtaaag
    12421 cttgccgagg attacggcaa aaaaagcagt aagggggtaa atctggacat taacatatca
    12481 agacaggagc ttgcaaatat agtaggtact accagagaga cagtaataag agcacttacc
    12541 gctttcaaaa aagagcactt gataggaatt gaaaagaata caatcacgat aattgacctg
    12601 gaaagcctca aggaatggcc ttattaaatt aagaataaaa caaatcggta ttgaattttg
    12661 atactcggtt tgttttttta ttgtatagga aagtatgctt tcctatacaa tgggggatgc
    12721 aaggaagagg agtcccctgc cgatgaagga gggtgctcag ctgcagagct gacgccttcg
    12781 ggaaccatag ggttccctag cgaaaaaaat gcgtcgggag acgtgttttt tatgaaacca
    12841 ggagtttttt ttcgtctaaa acacaggagg gcattgaggg taacgggaat accggttgta
    12901 tataaattgt aatattacat atcggtctta gtgtaaaatt gttgtaggtc aaaaaatagc
    12961 agagaccact ctttttggta aaatattagt tgcaaaacaa aataaacacc aaggaaggtc
    13021 tcttgtgaat aatattatac atcaaatttg tgagaattac attagtgaag ttttgcattt
    13081 tttcgattca ggggcaataa gaaaacttga tgaaatcgag gttgttctaa aggaaaagac
    13141 aaatagctat cttaaacaaa tgactcaaac atactttgaa gagatagatc aagctatagc
    13201 agacgataag acgggcggaa gatcttaaag tagggtttcc tgaggaaaag cgtagtatcg
    13261 agggcgttat aatctaaaca gcaggaaagc aaaccaaaat aaatacaaaa aaagagaaga
    13321 aaaagcataa tatatgcact gcgaccgacg gagccaggct tcttgtcaag gccgcgtgcg
    13381 gagcaccgtt cattttagcc ttgacaaaat ggcaagcggt acaataataa tatttttctt
    13441 tgtgtaatgc tgacatctga gaaaatctca aagaacggat gcaaggcaac ttgtgcgttt
    13501 acccggaata ctgatttagt taaaccataa agggagaatc tgctttttaa aaaccgagaa
    13561 caaattgaca ctgtcgatta taacagcgat attgcaattt tatagaaaaa atgggataat
    13621 atcaataatg atgcaatttt atagaaaaac atggtaatat catgttggct aaaaaacatt
    13681 tgataatgtc ggatattgga gtaatggcat tggaagggta taaattaagg tataagttaa
    13741 taacgtttct cagttccggt aatattaaag atatttcatc tgtttacgct aaaacatttg
    13801 atatagcttt atttattttt ccgagtagaa ggagttttat tccctttgat agaatagaag
    13861 ctatcatttc cgaaaccttg gaagtctatt cagaaaaaaa cctggtgcag gttcctccct
    13921 ttgataacct tgagcctacc attacaaacc tgggaaatgt gttttttgtg ctattaaaag
    13981 aagctctctt tcaaaaagat ttagttttag aaaccctgga aattagtgag agccctattc
    14041 gcagctatat agtcaatgaa gtaaatgcag atcggggatt ccttgtcggg cacaagaaaa
    14101 ttaaagcaac cagtttgcta atggatgata taatttctca atcaacttat catttgcttt
    14161 cggaaaatga aaaagaagga cagacgccaa ttcctgtagt tcatgctccc gcgccggtag
    14221 ctgaagcagg gcttcctttg cctcctccta aacccaaaat agaggattcc tcacccactt
    14281 tgcagcctgt taaggtaccg gtttatcagt tcaccctttg ttttctttta ttgacggttt
    14341 gtggagtact tattacaata tatttaaaaa agacaggagc gtacccttcg ggatcagaca
    14401 tatacggtca tctgttcaaa tccgattttc tttaccacag tatcagagca ggggacttct
    14461 atcctcttta taccgatctt tggtacaacg gtatgcaacc tttccgctat tgggcaccac
    14521 tgccttatta ttttctcgtc atactccaat ttatcgccgg gggcgatgcg atttcctcat
    14581 atctgctgtt cgtacttttt gcttttgtga taggaggaac cggatggctg ttatggggtt
    14641 gggcctataa caggatgctt tttactactt tccttggtgt gctgtggttc tttcttcctg
    14701 ataacatcag agttttcttt gttgaaggaa acttcccgcg tatggcaata gcaatttttc
    14761 tgccctattt gttctatttt gtatggagat atgtggaaca tagcaaaacg tgggctgttg
    14821 ttcccgtaat ttttataatg tgctgcatta tattatctca tgttatgatt gctgccatga
    14881 caggcatagg gaccttcatt tttttgttta tttacagtat gagacagaag cgcctgctcg
    14941 agtccttcca gataatcgtt gccatgctgt tatcatttgc actctgcgga gtctggttat
    15001 acccggctct tcaaggggga ctgatgggga tgaatgctgc tgcaacctcc gaagtcatgc
    15061 aatcactaag cacccccatt tcaatatcct tgaatccttt gctgcgtact caggagatat
    15121 ttgactatta ttattttgga ctttcaatcc ttgtgattag cattatcgga atttttgtag
    15181 ctgataaaaa aagcttaccc ggctttctta catcaattat tatatttttg ggaacaacaa
    15241 ctgttgtagt gccttttctg gagaagctgc ctttaaacca gttgctttgg atgatgcgtt
    15301 ttacacctat cgtatatgcc atatttatgc tggctctact ggaatggcgg aattgccgcc
    15361 gctatgttgc gattattata gctttctttc ttattattga cagtattccc tctgcacatt
    15421 tagagaaata tttttcccat aaccctgcca gattatctga ttctctttca actaccaaag
    15481 agcttacccg gcaaagagta tcattgatag atctcagtgt ttttgattct tatccgtctt
    15541 attatttttc aagtaaggaa ccgaaaacca aatacacttt tggatgggca tggcaagggg
    15601 ctgcgacagc aaacaatatt atgatgatca atacagcagc agagaaagga tactattact
    15661 atctttttga ccgcagcctt gaactgggtg atgacacagt tatggtgcat aaagaacttg
    15721 tggataaggc aaaaaggaca atgacttcac tgcttgaggc tgcacaggct tcaggataca
    15781 aattttataa agaaacaaac tttacttata tttttcatag ggatactccc gcttgttttg
    15841 gtgttgcttc cgaatatgcg gggctggcaa taggtaattc cgcaaaggca atagcgttgg
    15901 aatatccatc ttttgaagaa ggttccagtg ataacctaac ggattacaga tatgaggact
    15961 taagccggta taaagtgatc tacctttccg gttttagtta taatgataag aaggccgctg
    16021 aggagctgct gactcgcacc gctaatgggg gcgtcaggat aatcatcgat atgggtcgga
    16081 taccggttga ccctgtgacc agccggatga ccttatttgg ggtaacagcc caggctataa
    16141 gtttttctgc tcagtatccc gaattgatgt atcatgacca ggtttatcaa gcgcttccct
    16201 tcaaagaaga atactcgacg tggaatacct tttatttaga taatgtagag caagcaatcg
    16261 gatattcctg gtttaaaaac aagaatatgc cttttttagg aacggcgggc agcccaaaca
    16321 taatatttat gggttacaat atattgtttc atgcaatgga aacccaggat gagtcaataa
    16381 taatgctgat aaatgattta ttggagtttg agccgaccca acttccggac agggaagtag
    16441 tgtcgatttc tgtcaagaaa caaaaagaca agttgataat tgattccgaa aaaggtagta
    16501 ttaatacaac aattgcttat caggataact ttcggagtga tcagaaaatt tatgaaaaaa
    16561 ataacctttt agttgtgcaa gagccccaca cggaaattga aataacctac gcatacttgg
    16621 cccaaggatt ggctttaagc ggtgcgggag tacttggtac aggtgttctg ctgtatttca
    16681 ttatcaggaa gaggaggtgt gttcaatgaa aaaaataatt cagaaggttc ttataggttt
    16741 attacttata caggcggttt ccttttccgg ttatgcagac tatggttcta ttgatattga
    16801 tggttattat gatgattggg aggataaacc tcataccgag gtttattatg gtaagaagcc
    16861 ttatgtctcg gaaattcacc tggtttctgt attccgggat gaaacaacgg tatatgtgca
    16921 tataaaaatg tccgagaacg ggtatacaga actaccaaac tcttatttta ctatggatac
    16981 agacatgggt tcagaagctt ttacgctgat gcttgattcc atgaagaaaa aagagtccgg
    17041 tacagccgga attgaagttc atctgacaga taactgggat gtagtcggtt cggggtatta
    17101 taccagggag gaaggggaat ccgatgaagc ggagtttgaa atcccacttt actcaattac
    17161 agatgagcct gatggagtag ttgatatcgc aattatgttt cccgatttgg gatcgcagga
    17221 tattgtatgt gtcggtgccg gaacaggacc atatattgcg gttgcaatag gtgcggttat
    17281 cgttttgctt tcccttgctt attattacaa aaagaagcat ccggcttatg ccaagtgaaa
    17341 ggaataatca atgctgactt tgaaaatcat tgcgttcata gtttggatat atgttatttg
    17401 ggttctcagc aggacgggac ttcacttttt taagttttgc ataggcagtg tcggactttt
    17461 cttctttttg atgctctgga tacagccctt aatcatgcag cccttaaaaa tggcagtatc
    17521 tgctgctgct ggcgtattgg gagatttttt tgaaatgtat gactcatatt atcaatactc
    17581 aatattattt attccaaaag atacgaccgc agtatccctt acaattgatt atgaatgttc
    17641 aggggtaatt gaaattatgg ccttcagctg tatgctatgg ttctttccgc tttataatac
    17701 aattgaaaaa ctgataatca attgcatagg gttggcagtt atatttttag ctaatgtttt
    17761 gagaattttt ttgatttgca cactgattta cttttacggt aatgagattt tctatttcgc
    17821 tcataccatt tttggcagaa tatttttcta ttctatttct attgctctct acttttatgt
    17881 atttacccgt tcgcatatca tacggcagaa ggtaggtagt ttcaattatg gaaatggaga
    17941 tactgataaa tagaataatc agagaagtaa tcttctggat ggcctggata atcatccctt
    18001 taattatgga gattatccct gcttttggag gtttttttat actgctaaaa aagagctttg
    18061 tactcaaagg taaagccatt ccgcaggctg tgtcggaaat taccctgatt attccgatat
    18121 ataattctgc cgaaaccttg cggacttgtt tgacttcagt ccacaattcc aactatccca
    18181 tcgaattgat ggatattctg ttagtgaata acgggagcac cgacaatagc tatgaggtat
    18241 acagacagtg tcaggatgaa ttcagagacc tgccgatgac ctggctcaat gccagtcaag
    18301 gtaaatccaa agcattgaat atggcactat ataacagcca cggtaaatat attgtccaca
    18361 ttgacagtga cggaaaactt catcccgatg cactcagaaa tatggcaata cgattcgaac
    18421 agaggcctga ggtagattgc cttacgggag ttatattgac agatatgaag ctaatcgaat
    18481 cgacaaatag ttttatgatg aaattagtaa ggcgatgtga attttttgaa tattgccagt
    18541 cttttttagc cggaagaaat tttgaatcgg aattcaacag tatttacacg ctttccggag
    18601 cattttcggc ttttcgtaaa tcagctattc ttaaatccca gctttttaat acagctacgg
    18661 taagtgaaga tacccaggta acgtttcaaa tgcacaagat acaaaaaaaa ggcattagtc
    18721 tttgcgaaaa cgctttcttt tatgttgatc ccattgaaag ctataataag ctttataccc
    18781 agcgccagcg ctggcaaaga ggtgagatag aagtatccca tatgtttttg caggaagagc
    18841 tgactataat aaaaggtttt attagcaatt ttatggtgcg tacactggtt tttgaccata
    18901 catttgcatt cccgcgtatg atatggtatt ttgcacttat tttccttgta tttttaaact
    18961 atcctattgc cttggttttg agctctatag gaataatata tctgctatat tccttttcca
    19021 tgtttcttta ttatatcaat gtacttatgt atctggataa ggtaaaagat attcagaggt
    19081 attacaggtc gaagtggtat ctcatatttg tattgcccgg gtttaacttt gtggtttttt
    19141 ggattcgttt tgccggtatt attaacagta tcaaagggga gggagtgtgg aaaaccacat
    19201 ccctgaccga agaatggaat aagataaaag ttgccgttaa tgacgacttc cactttccca
    19261 aaagagtgat cgcggcatta agaaggaagg ttaatctatg aatacgctga ctgtggcttt
    19321 acccgcctat aatgaagaag aaaatattga agctttggta agcagctggc agcagtatcg
    19381 ggaaatactt atggacaagt atgggttgat attacagatt gtcgtcatca atgacggcag
    19441 taaggataag actgtcgaga ttgccgaaga tctgaaaaga aaatattcga acttcacatt
    19501 ggtcaatcat atccataata aaggtcttgg agaagctgta aaaacaggga ttgcttattt
    19561 tcttgacaat tgtcccgaaa gccggtatat gtgtctgatg gactgcgaca acacacagga
    19621 tccgtgttat ataaccgata tgcttgactg cagtaatcga accggagcag atgtcatcat
    19681 tgcttcccgt tatcggaaag gatcggaggt tatgggggta tccggttttc ggctgattat
    19741 gagcggaggg gcgagatatg ttttttcggt tctgctgaat attccaggtg taagggatta
    19801 tacctgcggc tataggcttt acagcagaga aatccttcga aaggcctcat acagatttga
    19861 gaaggattta attgaggaaa gcggatttac ctgcatggct gaactcttat ataagctttt
    19921 tacctgcggt gcggtctttg gtgaaattcc ctttgttctg cgctatgact ataaaaaggg
    19981 aacaagcaaa atgtcagtac tcaaaacagc tataaacagc atgggtttag cgctgcgact
    20041 cagaaaaatc aaaatgacgg agaaatcaga tgagtgaaat tacagaaaac aagataacag
    20101 atagcaaatt gcaataccgt atagagcttt tttcggctct gattattaca ggtattgttt
    20161 gtgctgccgg ttcttccaat ctattatttg catcaaatga tttgtatccg gcggcaacag
    20221 atgctatggg tcacatggca aaggtacaat atattaccca gtgcctgaag ggaggggtta
    20281 ttccttcatg gtttccttat tggtataacg gtgccgccgt cactcagtat tatccgccgc
    20341 tttcgtatta tttaatggtg ccggtatatg ccctaacggg aaatgttatg cttacattca
    20401 aaatcaactg ctttctgatg atgtttatcg gtggcatggg agtttggtat ttttgccgta
    20461 aggttatcgg gaaatggtgt ggttgtatag ctattgtatt ctactgttta cagccctttc
    20521 ttttgagctc attatacatt gtcggagtat tagcacaggg acccgtctat gccataactc
    20581 cttggctgct atatttagtg cttagcttta tcaaagaacc aaaccgccag aaagcactct
    20641 tttgcaccat tgctactgca ttgctgattc tatcacatcc aatgcatgca ttcataattg
    20701 ttctttgcat aatgctagtg cttctgatgt ttattccttc taaaaggata tccttactta
    20761 attatactgt gttggtagtc atgatgacat tttccttaat tataacggct ttttggtctt
    20821 tagtcggagt aacgggatta gaaaatccaa caattcccta tatattacca ggcgtcgcag
    20881 caaatgtcac agcgactctt aaatggttcc aacccggaaa catgggtggc ctatacttct
    20941 attttgcact gccaattttg gtatttactg taattgcggc aatggcttta attttgcgca
    21001 ctacaaaaaa gcaggttatt aatgctgaaa ggttcgagat tccattttgt ataacattaa
    21061 cattcttcac tactatgttt tcttttggca ggcaaattcc aggattcaag cttctaccta
    21121 tgacagatag tttggtatcg ggtcgaattt tgtctcttac tgcacttacc ggagcaattg
    21181 cctgtgccta cctaaccttt acagttatag ggtatatacc aaccttgcga aggatgatgc
    21241 gtttagtaat tttggctatt gccctcagcg cctttgtcct gacaattgtt cacattaatc
    21301 catataaatt ctcttttccc atagggtcct atgaaaaatt tgaaaaacgg tttgaatctg
    21361 tagccggaga tatttccgtt tctccttttg acaaaggacg ttatatctgg ttcagcggac
    21421 taagttctga tgaagcaaac atacctatag cttatggttt tactatctca gagggatgga
    21481 atatggaagg aagtcctcac aaccgtgctt tgtggaatca tctattatgt attgccacag
    21541 atacaagaga ttatctagct aagaattttg ctttctggaa tgtaagatat tttatgttgc
    21601 ctactcaata tgatgaaatg gtaataccct taaaagagca tcttggtttt cgattagctt
    21661 acgacactga gttttataca agccaattgc cgtcatccta ttttctaacc gacaaccgca
    21721 atgggctgat ttttggtatc ggagcacccg gagttgctat ggagtttccg tatcttgtct
    21781 atgaacagcg tcgggatatt tctgattatt cgcttgcaga actttcagaa tacaagctta
    21841 tatatttatg cgagccggtg gtggatacca tgaggcaaaa agaaaggatc gaagaaacaa
    21901 ttaaatacct gatagataaa ggagtcagaa ttattattga acctacgcca accaaaggat
    21961 ttaaactatt tgacgtggca gtctccgatg tgcctgctga ggactcactg ctactggaga
    22021 aacaatacat gtccccttta aatagtatta tagataatgt gaagattgat gagaaacatg
    22081 ggtacttcag ggcactgttt ggacttgatg aagcgtatta caaatcggtt caaaatggcg
    22141 gactgttgca aaatgacatt atcgggacaa aaagagtggg aaaaggtgaa gtgctttttc
    22201 tcggaatgca tctcagtcag tatctgaaag ctgtatatgc attgaacctg ggtgtacctg
    22261 aagatattaa aggatatcca agctgctcag atgaaattaa ggctttattc ggggacattt
    22321 tcaaaaccta tggggttgaa cagaattttt ggccggaacc ttttcctgta aataatgttc
    22381 gctggaatta taagggtgtt gatttcgatt attccagtca ggcagcgcag gagatgacta
    22441 ttagtatcac ttatacgcca cgctggaagg cgacaataga tggaaaaccg gtggatgtcg
    22501 ggcagcgtga aaacttgata acactgcagc tgcctgccgg cgagcataaa gtgcaattaa
    22561 aatatgggtt gacaaaatac gctgtcatag gatacactac attgatagcg ggattgttac
    22621 tattttctct ttttttaagg aattatgaca taaatgttat taatctaaga aaattatgtg
    22681 ctttattaaa gaactatttg caggtataaa atcatctttt tgttttatag aaagaagttg
    22741 agataaaatg ctgaaaatag gaaaaattag ttacaaagtt aagctttttg tcatttcctt
    22801 actgacaata ggttttattt cgatttgctg gctatatatg tttcaagagt tgaataagta
    22861 caaggacagt attatcaaaa actatagcag tactcaagaa agtctggtta cagaggtctc
    22921 aaaaaaagtt aaagaaagcc ttttaaccgg ctccggtgaa gaaggtgtta ttgatttatt
    22981 aagtaatgcc ggaacttcag ggagcagata ttggttttta tatacagctt caggggcggt
    23041 atttgaaaaa gactatgaag aaaccaggaa aattaaaggg aaaagcgcat cggagttaat
    23101 taaattttgg aagcttcagg gaggcaccgg tacggaagat tttgagaagc tgcttcttca
    23161 gaagaaaaat ggatcttctg tctttacaaa aagtgcagat tatggagaag agattgtttc
    23221 agtaaagtat tttattgcaa ataataccgg atacttttta ggaatgtcaa cacaacatca
    23281 gtatgttttg tatgttggaa gggtcaatga acatattcta tatttatata tatttgcagg
    23341 tgctgtcagc ctggctcttt tagtccttgc tctttttctg tgcctgactt tgtataaggg
    23401 tcagaagcga tgtgataaag caaataaaat aataaaagat aataacttcc acattgagga
    23461 tcttacaaaa aagcttgcct cgaaaaccga tacagtgaaa aaagcgagta tatttgacga
    23521 cctgacaaag cattataata ggaagttctt ttataatttg ttgtccaaga taaatgatga
    23581 acattttcag cccataagca ttgttgtttt ggatatcaat gggatggata ggctaaaccg
    23641 aacagcatat tacagaacag gtgatgaggt tcttgaaaaa acctccggga tattgcaaag
    23701 attatgttta gatacagata ttgttgccag aaccggcggc gctgaattca gtgttcttat
    23761 gacagttaca aatatatctg aagcttacgg gataacagag aacataagac gtcagtttac
    23821 aggtcttaat catgccgatt tgacgttatc aataggcgtt gctcaaatgc aaccggaaga
    23881 taacagcatt tttagcgctt tggaccgtgc acgtaaaaac ttgattttgg aaaaactgaa
    23941 tgataaaaac agcagcgatt atagtattat cgcaatgttg atggaaatac tcggagctta
    24001 ttctctttca acagttgccc attgtaaccg actgagagaa aaagcactga taatcgggaa
    24061 atcccttagg ttgtctaatt cagaactctc acgtcttgca atagccgctc agcttcatga
    24121 tgtggggaaa ataggtattt ccgatacgat tttgaacaaa aaagaaatgc ttacaagaca
    24181 tgagaaagat tgtatttaca ggcatccgga cattgggtat gaaataataa aattgattcc
    24241 gcctttagat gaggttgcta ttgatatccg gcagcatcac gaattctatg acggcaccgg
    24301 ttatcccgca ggacttaaag gagaggagat ttccctctgt gccagaatta taaatgttat
    24361 cgatagcttc gatgcgatga caaacaaaag tgtatatgct aaagtgaaga cgccggaaga
    24421 ggcaattgag gaacttcaga agaaaagttc cattcagttt gatccccata tagtagatgt
    24481 ttttgttaaa gtatttagcg acaatataaa ggtaggcagt gagattgcat gatgaatatt
    24541 ttaaagcaga ttctgaagga taaatttgca ttatcaaaat atttcttata ctcagtgata
    24601 gttgctgtta tcgatactat aattgtttgg ttactgacaa ggcagaattt gactcacttg
    24661 gttatggcaa acacaatagg cgtatcaata ggctttgttc ttcattattt actatcttca
    24721 aaatcagtct tcaaaacacg atacagtgtt atagggttta ttatctattt ggggactttt
    24781 tttgttggct tatttgctgc caactggctt atttatgtat cttaccaata catctttgtt
    24841 gattatatga tggatttacg tatcttgcta agcaaaggag tctcaataat agttccgttc
    24901 tttttaatgt attttttgag aagatatctg ttttttattc ttaatagaaa ggaagacaaa
    24961 tagtgacata cagtcaatac cgattcaaat tttatttcaa tgcaagtcat gcaatatacc
    25021 tgatggggca attgggggag agtcatcctc acacatggga attgattata catggggcca
    25081 agataactga cgactttgca ccttttaatg agattgagaa gaagatagaa gattttcttt
    25141 cacaatatca ggatgtattc attaatactg tacagccatt taccacaatt aatccgacac
    25201 tggagaatat atgcactcat tttaaaacaa gtattcaaga gctgctttac ttaaagggat
    25261 ggcttcttct ttccatagaa ttaagcgaaa cgcctacaag ggcatatgtt ataagtatgc
    25321 atgatgaagc cgaatcaaaa aaatcatatt atgaaaaagc cggcgcagaa acatatcagg
    25381 atgttgtgga cgagcttgtt tttgagaagt tggattcctt atttgaaaac agggaaatag
    25441 ctgccgaaaa agatactgac ggtgaacaaa aataaaaaga catatgaatt gcggaaaatg
    25501 tgacctgcca tttgaattcc aatgtaaaat gtttgacagg tcacatatag cattaaattt
    25561 tatgatacct gaaactaagg gtcttttttt tcgtctaata ttaaagtagg gctcgggggt
    25621 taccgggaac atgtactgta tagaaattgt aatattacat atcggttact ttgctgaaag
    25681 tacggggata gtctggtaaa ataggttata caatagcctg aacaatggtt caagaaatct
    25741 aaaataaggt aaaagtaaat gaggtagtga ttatgaaaag aattcgtggc agtagatttt
    25801 taattaaggc attggttata ctgcttatag catctataac atttccggga gcggtttatt
    25861 ctctggaatc ggccaaactg attgaagacg ggcagcacat tggccccggt acatattata
    25921 ggaatatgaa ttacataacc cctaacggga actttatggt caacatgata gagagtaaat
    25981 tcgatgacaa atatatcagt gtagaagttg ctgacagcgg aagcagcctg accaacaaac
    26041 cggtgtcgta tcaggcttcg ctgaaaaatg atgagaacaa gagagtaata ggtgcagtca
    26101 atggagattt ctttgacatg acgacaataa aagggcttac ttacggtacc agtattatag
    26161 aaggagaaat caagactgca gtaaagactt caactatact tggagttacc gacaagggag
    26221 attgcttcat cgataccttg aatatggaag gggctgccgc atataagtac atacaatatc
    26281 cggtagactc agtaaacagg ctcagatggg cggatcagac cgttatttac actccatttt
    26341 tcggaaagac aacagtaaat acagttgccg ggactgatat catagtaaga ggcgttgaac
    26401 ttccgcttaa agctgggaag agatatacag gcaaaattga aaagataatt ccggactcca
    26461 aaatcacaga aataccttca gacggagtgg ttatttccat gcctgtcaaa gccttcaggc
    26521 agcttgccgg agttccttcc ggtgctgaga tgagctttta tgtagatttt gacaagaaaa
    26581 acctggattt tgcagtatcc ggggcgccca ggcttcttga aggggggcag atctctgcga
    26641 aggtaaagga aagggcagat gccttgcaaa ggcacccaag gactgcgata ggcataaagg
    26701 ataacaagat ttatatggtt acggttgacg gaaggcagct tgggtacagc gacggaatga
    26761 ctctttatga attggtggag tttttcttga gccaaggagt ggaaaatgcc atcaatctcg
    26821 acggcggagg gtcaacggcc atggctgtaa gaaagcaggg tgatgctgct gcaaagctgg
    26881 caaacaaccc ttctgacgga agggaaagat atgtgggaaa ctcaatacag cttgtatctg
    26941 aagcaccgtt atcagatcct gcaatattga gattcaagga taccagtgtc aagatatata
    27001 agaacagctc cttcaagcca tcctattacg ttttggacaa gtattacaat cttctgccgg
    27061 gagatatggg caaagtaagg ttcggaattg atgtgaagac atcaaagatt gctgcagacg
    27121 gcacatacag ctccggcagc caggcaggca aagggtatat atatgctgcg tgcggggata
    27181 cacaaagcaa aatgcttgtt gaaatagtgg acaaggtaag ctcgcttgcg gtaacaaatg
    27241 attttgtgct tcttgatccg ggagaaaaga cacaactgca ggtaaaagcc tttgatgaaa
    27301 acggcgggaa tattataata agcccctctg ctgtcaagtg gacagttgaa ggtgaattgg
    27361 gaacggtgga taaagatgga gttttcacgg caggaaataa taatggacat ggtaaaataa
    27421 cagcttctgc aggaactgtc acagcagccg ttggggctaa gataggcaag actcccgtaa
    27481 ttgttgccga ctttggaacc cttgacaacg tggaagcaaa atttatcaga agcaaggcag
    27541 ctataagaca taatcagaag ggggaaccgg taaggattgg aaatatttcc ttaagacttg
    27601 attatgattt ccaaaatact gaaggtacgt cagccgctta tgtaaccttt aagaaaccgg
    27661 taacaattat aggaaagcct attgagcttg gagtctgggt atatggtgac ggaagcagcc
    27721 actggattag aggaaattat ataaactcgg cgggagagaa aaaggtattt gatctcacca
    27781 agtccggagg ccttgattgg aagggctgga agtatgtata tgctgaattg ccgaaagatg
    27841 agaaataccc aatagccctt gagcagatat acttggccga gacagctcag gataataaga
    27901 attccggcag catatatttt gacgatgtaa tggcaatata caaactggat aaagactatt
    27961 acgatcctgt gatagtatcg gtactgtccg aaagcagtga agggcttccc ggtcaggctc
    28021 aggagatagg tgtctttgca gctgacagag gttacggcat cgatcctgct tccataaaga
    28081 tgtatattaa tgattctgcc gtaaaagcca gttttgaacc tgaaacagga aaaataagct
    28141 acattcctgc aaagccgctt aaaaaaggtg aatacaaggt gagaatatca ttgaaggata
    28201 agatagggca ttccctgaat ccggaatatg cctatacctt cacagtcaag taagagaaga
    28261 acgaggacac ggggacgttt cgcgtgtccc actggaagtg ggacagggga aacgtccccg
    28321 tgtccttttt tcgtaacctt acttctttac cgcttccagc ctgtttatct ttatcccctt
    28381 tttaatgaac ttctcttcat attccgtggt gatattcccg ggataatcct cattgtgaag
    28441 gtcgtaagtc agatcaagta tttcaaagcc ggaggccttg aattcattta tagaaaaatc
    28501 aaaaagctct ctgctgtctg ttttgaattc tattcttcct ccatttttca aaagggttct
    28561 gtactttccc aggaagagcc tgtgagtcag ccttcgcttg gcatgcctga ccttcggcca
    28621 cggatcgcag aagtttatat agaaagtgtc cacttcagaa ggttcgaagg catcctcaat
    28681 gtccaaaatg ttataaggta taagaattac gttttgaagc tccatttcaa gcactttact
    28741 cagagctgag agaattatct catgccggag gtctattcca atgaaattta tatctctgtt
    28801 aagcaatcca tttgttgata tgaatcttcc cctgcccatg ccaagttctg cgcgtatcgg
    28861 agcatcctct ttattgcctg ttctccttga gaagtattca ttccaattgc ccttgtattc
    28921 ccccggacgg aatatgacga tttcatcata ttgctttata atctcaagag ctttgggatt
    28981 atttcttatt ctcatttgtc ttctccacct gcctgccgta gttatattac aattattatt
    29041 tatagtaaga gcattgtcaa tgtatcacac aatacattgc tttgtattac aaaaataatt
    29101 atatcagttg ttattaaaat aacataaaaa gcaatagaca aaagacattt tagtatgtta
    29161 ccttaaatat aaatagaaga ggaggtgtta tagtgaaaaa aatgcaattg tccactaaaa
    29221 tcttgattgg ccttgctgca ggtatacttg caggtgttct gcttcaaggt tctcctgaaa
    29281 ttgctacaac ttggattaag ccctttggta ctttgttcct gaaccttatc aaaatgataa
    29341 tcgtaccttt ggtactggca tctcttgtag tagggacatg tggactgggg gatgtaaaga
    29401 agcttggaag aatcggcgga aaaactatgg catactatct attaaccact gcatttgcag
    29461 taattttagg acttattcta gctaatatgt tcagcgtagg tgcaggcttc gtgcttccgt
    29521 cagatgtaaa agcagcagaa gccagcaaaa tgccaagcgt aattgataca ctgcttaata
    29581 ttatacctac caacccgata aaggctgcct ctgacggcaa tatgctccag ataatcgcat
    29641 ttgcattaat actgggcagc ggaatagtag tattgggaga caaaggcaaa ccggtatatg
    29701 aattctttga ttcacttgca gaaatctcat ataagataac agggggaatc atggctcttg
    29761 ctccttatgc ggtattcgcc ctcattactc ctgttgttgc ttcaaacgga cccaaggtgc
    29821 tgctgccgct cttgagtgta atcattattg tttatgtggg ctgcgcactc catgctgctc
    29881 tggtatattc cggcacactg atgctatttg cgaagttcag tcccatgaag ttcttcaaag
    29941 gaatattccc tgcacaggct attgcattta ctacctgcag cagcagtgca acactgccgg
    30001 tgaatatgca gtgcactgag gagaaccttg gtgttccaaa gagtatatgc agctttgtat
    30061 tacccttggg cgcaacgata aacatggacg gtactgcttt gtaccagggt gtatgcgctc
    30121 tattcatagc aaacgtttac ggtttggatc ttaccatggc tcagcaggct accattgtat
    30181 taaccgctac cttggcttcc ataggaacgg caggtgttcc gggagcgggg cttataatgc
    30241 ttacaatggt gctgcagact gtcgggctgc ctcttgaagg cctggcactg gttgccggta
    30301 ttgaccgaat actggatatg g
//
LOCUS       DAOOAB010000032        28582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2158_length_28582_cov_14.682056, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000032 DAOOAB010000000
VERSION     DAOOAB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 28582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28582
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_28582_cov_14.682056"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..922)
                     /locus_tag="PKU88_08105"
     CDS             complement(47..922)
                     /locus_tag="PKU88_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69275.1"
                     /translation="MKKSYWWIVISYGAFLGWLLSFPYNGPVLRAISAAANTAVEPLS
                     LIYTFVPVLFLFVFFIYKPKENHLKTIMILCIFVCLTGTLIIFLSKPHWWYFTLGIMG
                     ICSVIYIITWSSYYTRQIPINKKIKIMALVIIVGNIIYYLINIGLEYFTFNTILFILT
                     GVLSLSFWASYNLKVDNPIEIHFESEPFPTKLILTVCLFLFIIKLNGGIAIYVIYPSY
                     KEMYGSLSAYYGMLPYILVLLVLYCFSNKIPRMQYVYFGTSLMGMAYLSYGIMQNSIL
                     GYFLTETLLQMGWCE"
     gene            complement(1044..2417)
                     /locus_tag="PKU88_08110"
     CDS             complement(1044..2417)
                     /locus_tag="PKU88_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69276.1"
                     /translation="MKRLLIFVIAALIISSFVCYAAVEQNHHIDWDRVLFEGEKLGHF
                     VPGNIYADNQNNAVLYSKKTINTLTQHKIYRFNSSKDFDAISKWDETADYINTDFTSE
                     YRPTSDGGFIRQRQSSKVSMPPSSKKYTMEKSYIDKIDANMKQLFSICLQDDLKLPLP
                     GAGPASNINSWAEVEDGYIFVGTAGGSKAVKAADAWSSNVWFFKIDLKGKLIWEKVIG
                     SDTSQWLLDIVKAHDNSLILVGRSGNQIYLIRIDSNGNILGEKLLDAGLLVNNPRNSS
                     AKNCISMTNDGGFVMLTQTVEDASYKSGNITYSRSNVKYQITKYDYQFKSIWQNEFYG
                     INHIDYKKYPYEIKIYGIEALSNGDIVAFGQYKPRDKWMMSRFDPNEKYQAYIKEMDL
                     SGKDAGYNLITSGWDENYGILDIKEINGNLLFLGLRYKSSTANDYTWIGKLKSETASQ
                     TNGESAK"
     gene            complement(2475..3884)
                     /locus_tag="PKU88_08115"
     CDS             complement(2475..3884)
                     /locus_tag="PKU88_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69277.1"
                     /translation="MKIKRSYFTKTKIVATLLIVSIVLQAVFMTSFASENTDSLINTP
                     IAAGNKHNLIIRKDGTVWGWGYNRYNQLGLGRTTSRSVPTACQTVEIDKVIGVSAGKL
                     HSICLKEDKTVWAWGNNAAGQVGQGSPTLTKPYSIIKPVQVTNLKDVIEISAGDEYNL
                     ALKQDGSVWSWGNNIMGNLGDGTNATRYAPIKIETLSDIVHISAGNWHNLAVKADGTL
                     WSWGYNEKGQLGNGGSGGMFGVFNTGIDQNAPVQVLYVEDNTKDELPQPFTDVKTASA
                     GLNFSLALKNDGTVWAWGRNDQGQLGIGDWNDSDLPKQVMDLNNIVMISAGQNFSLAL
                     RNDGTVWTWGRGQYGQLGQFTEVTGPKKENGEPANGYTFIDGYHHFLDRNKPIQIKGL
                     SDVSYIAAGDDHGMALDKKGMLWTWGFNDEGQIGNGTVANRGYPLNNVKASIAGGPFT
                     NLDNILQPNISWPSTKVVP"
     gene            4550..5170
                     /locus_tag="PKU88_08120"
     CDS             4550..5170
                     /locus_tag="PKU88_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69278.1"
                     /translation="MLYLENIKPLDISRLLSSLGKPGARVDTDNQNPYSDKIIQGYYI
                     LLHTLFDKAVKWEMMSENVCDKVDKPSIKKHKAEFYDEEQINNLLECLNSEGEELELS
                     LSKRNPNNRSKSEQEKLELMEERKYLYSMHKAYVYLALASAGRRSEICGLDWTNINFD
                     TNYIVSDKTLQYTKEAGKFSRPYLKNGDTYKGMYLPEVVIDILEEY"
     gene            complement(5375..6907)
                     /gene="spoVB"
                     /locus_tag="PKU88_08125"
     CDS             complement(5375..6907)
                     /gene="spoVB"
                     /locus_tag="PKU88_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HPX69279.1"
                     /translation="MKKLTYIQGAFILTITNLITGSLSFIYRVFLTRAIGSEGVGVYQ
                     LVLPLYLLFITLVSGGITTAVSKLIAEQAPGSNYRNMYKIIKICGITIGIWSLALCSF
                     IFFNSEYLAKYVLKDNRTFFSIMVFTPAIFFISLSAVLKGYFYGLQYVKPPAMIDILE
                     KMIRLGGLLMTTYYLLPLGIEYVCAGVMAAMAAGEFISLILLYFCYKVKKAAVVHTKT
                     DSTIHIITRILKTALPLSLSGSLTTIMDMITAILIPMQLRAAGYSNSSALSIYGQVTG
                     MVMPLLFFPFIIVASLGITLVPAIAASYAAGNWTALNKKCNDSLKITSAIAFASTALF
                     IVFPWELCRIFFKCPEAGNMLFWMSFVCVFEYWQFTLFAVLNGVGKQHVVLKNSILHI
                     VISVICSYFLIPIPSIGIYGFVIGFCASAVLICLWNLADLKRIPNIHIDYVNIFMKPL
                     FAFVGMLIVIKCCNLYLVARTLQNFNMTVSSITGLVFFFLILFFMGIFTPKQLRNTFA
                     MK"
     gene            complement(7030..9255)
                     /locus_tag="PKU88_08130"
     CDS             complement(7030..9255)
                     /locus_tag="PKU88_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69280.1"
                     /translation="MLKKKLAALGIAVVFTLSSLTTTVAADYKKPAAGGNITIKVVSP
                     GKGNAYGQTKIKKNEVKKYRFDDCADITWALNAIEKLAGKGIIGGIGNGKYAPRSNVT
                     HVEALAMVMRLTGDDKEAEGMGKKVHPFYKGILPNWGYGYIFMAIEKGILLPEELKDF
                     NPNQPAKRHEIAKYIIRAMGETKEALKHMDEDLDFKDSSAVPDKSVGYVYLVSKLNIM
                     IGNEKNMFKPMEPVTRAEMAVLLDRAEGKFDLPDTDNRKNSTVFVSADVDDNEITVKV
                     KGVTVTYDVLEDVQVYRDGSFETIEDLVKGDILQLTLNNSKDVIFIEVVKSAEEGDDD
                     EEEEIAVSFRAVSDSSLPKVLKDEIDDMKSKESYAAYEYDNYIYLVAAMGRQRTGGYD
                     IEIEKVYRIGDNDEYTIRAVVDTDEPSSGSNVTQAITYPYSVVKFKSFDDIEEVIFVD
                     EDDDELDDVEIEDLDEDPEETKVSFAEVDYDDLSAVLKDQVDYLKLNKNFKAYEYDNI
                     IYLIATMGKKNSEDYKISIDDVYKVENGDKYTIKAVVDTTVPASSSGSKSVYPYSIVK
                     LRNFDGIDGVRFVDEGNNKLSETKIVELDEVVTAEGIISAINTSGKQIRVKKSNGTTV
                     ALTIPSSAEITVNDDDDASISDFEVGMKVKVDIVGDIVTEVIAEDDKTEVTGTLTGIS
                     ISTVKKITVKVDNTTKTYTVDSDVKVIIDDETERVEDLRVNDELTIKFINGILAEIEK
                     K"
     gene            9567..10511
                     /gene="pyrB"
                     /locus_tag="PKU88_08135"
     CDS             9567..10511
                     /gene="pyrB"
                     /locus_tag="PKU88_08135"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPX69281.1"
                     /translation="MSLKGRHLIDPNDFSLSELDEIFDLADRMADDRDSFGNICNGKI
                     LGTLFYEPSTRTRLSFESAMLRMGGKVLGFSDAGTSSVSKGESIADTIRVLSSYADVV
                     VMRHPKEGAPKLASEYSLIPVINGGDGGHQHPTQTLTDLLTIRRYRGTLENQTIAFCG
                     DLKFGRTVHSLISAMSYYKGSKFVLISPAELRLPGHLKSSLIEKHNVGIYETENLEEV
                     INQVDVLYMTRIQKERFFNEADYMRLKDSYILSRSKLKDSREDMLVMHPLPRVNEISY
                     DVDDDKRAVYFKQAEFGIYVRMALMALLLGVNVNVNRK"
     gene            10495..10926
                     /locus_tag="PKU88_08140"
     CDS             10495..10926
                     /locus_tag="PKU88_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPX69282.1"
                     /translation="MLTVNSINCGIVIDHIAAGNGIKIFEKLKLDEVDFPVVLLINVP
                     SRKLGRKDIIKIENKIDVDLTMLGLIDPNITVNIIENDKIVKKMNTIIPEEVQGLFKC
                     KNPRCVSNFDEYVTPKFHLAKANGKIHYRCGYCEELTTYSI"
     gene            11087..11506
                     /locus_tag="PKU88_08145"
     CDS             11087..11506
                     /locus_tag="PKU88_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPX69283.1"
                     /translation="MKMPEFSIGMSKSVQKVITREDTALNFGSGALKNLLATPTLSAL
                     MIEATVKTIDPLLPDRYITIGKSIEIEHEHATKEGTTVTVSAELVEINGNVLSFKITA
                     FDEIGRIGTGYHVRQIVNYDILMKRVEERFSVMEGRP"
     regulatory      11591..11725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            11832..12467
                     /locus_tag="PKU88_08150"
     CDS             11832..12467
                     /locus_tag="PKU88_08150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX69284.1"
                     /translation="MSVATKSYSVVNPYHLKKLLRVRKAEISDINSIYDVASSVGTSE
                     KISEQGFLVDDYQSKPNYYKKKIGHWIENLERFYVAEYDKVYGFMIAYTKEEWLRDNP
                     SWLQDVYWNPEFDPSWTNKFIVVDKTAVFADLTGMGIGSLIYKRLLEDLRNEGIKHIF
                     AETIISPQPNFASLQFRKKQKYSLAGVRYEKYNNILYTDLIYHKSAFDALT"
     gene            complement(12543..12743)
                     /locus_tag="PKU88_08155"
     CDS             complement(12543..12743)
                     /locus_tag="PKU88_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPX69285.1"
                     /translation="MARNNQKVVPQATAALDRMKFEVASEVGVNLKEGYNGDISARDA
                     GRVGGTMVKRLIEQAERSMSGR"
     gene            12903..15503
                     /gene="clpB"
                     /locus_tag="PKU88_08160"
     CDS             12903..15503
                     /gene="clpB"
                     /locus_tag="PKU88_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPX69286.1"
                     /translation="MDINKLTQKAQEAVIESQSLAIKHNHQTVEGEHLHYAIILQNEG
                     LIPKLLSKMNVPFDFYYKDISDLLDKIPKIYGETASSPYVSRRFEQLFLRADEEAKKF
                     QDEYISVEHIYLALIDETNTPSSKIIKKYGITREKFLRELSAVRSNQRITGQNPEETY
                     EALKKFGRDLVEDARKGKLDPVIGRDNEIRRIIRILSRRTKNNPVLIGEPGVGKTAAV
                     EGLAQRIMKGDVPEGLKNKTIFSLDMGALIAGAKYRGEFEERLKAVLKEIEKSNGRII
                     MFIDEIHNIVGAGKAEGAMDAGNLLKPMLARGELHCIGATTLDEYRKHIEKDAALERR
                     FQPVLIDAPDVEDTISILRGLKEKFEIHHGVRIQDNALISAAVLSNRYISDRFLPDKA
                     IDLIDEAAAMLRTEIDSMPGELDEIMRRIMQLEIEREALKKERDKQSEERLEALNREL
                     SELKSKGDSMKAQWELEKENIKNIREIKKRIEETKAEIEKAEMKYDLNRVAELKYGTL
                     IELEKQLNAEKANLEKMQGMGQLLKEEVTEEEISDIISKWTGIPVKKLMENEKEKLLR
                     LDDILHKRVIGQDEAVSAVADSVIRARSGLKDPKRPIGSFIFLGPTGVGKTELAKTLA
                     EALFDSEDNIIRIDMSEYMEKHTVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVLLFD
                     EIEKAHHDVFNIFLQILDEGRLTDSQGRTVDFKNTVIIMTSNIGSMYLLEGVDNDGEL
                     KPEAVEAVNEELKRHFRPEFLNRVDDVVFFKPLLKEQIRKIIDLIFEQVAERLKDRNI
                     KISLSENAKDYIIGNSYDPVYGARPLKRFIQRKMETSIGRALIAGEINDGNDILIDAD
                     EKELKFKKLS"
     gene            15602..16015
                     /locus_tag="PKU88_08165"
     CDS             15602..16015
                     /locus_tag="PKU88_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPX69287.1"
                     /translation="MGKTYEQINVGDKGSFEKTISETDIYMYAGVTGDINPAHINHEA
                     MKNTKFGGRIAHGMLTAGLVSAVLGMQLPGPGTIYLSQQLNFKAPVKIGDTVKAVAEV
                     VEKLEKGRVRLKTSCYNQDGVEVLDGEALVIAPRE"
     gene            16030..16371
                     /locus_tag="PKU88_08170"
     CDS             16030..16371
                     /locus_tag="PKU88_08170"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPX69288.1"
                     /translation="MQSVIRNQKDVEPVKNPKHNEVYGRLLAKQEENGRLSAHLTNIK
                     PGGEILPHTHEVLEVFYVIEGEGLALVNGVRQKAEKGTIIAANTGSEHGIINTGESDM
                     VLFCVFSPGIA"
     gene            16631..17623
                     /locus_tag="PKU88_08175"
     CDS             16631..17623
                     /locus_tag="PKU88_08175"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPX69289.1"
                     /translation="MFEIRLTENEAGQRADRFLRKYLKEYSLGDIYKLFRTNKVKLNG
                     KRVKENQMLKEGDLLQLYVEKPERAAKTAIPLVKPVGGIDLIYEDANIIIADKPAGLL
                     THPDKPGDTDTLLDRALYHIYGEKEADESRTFSPAVCNRLDRNTSGIVIIAKNYKTLK
                     AVNHSIRERGIRKLYLCIAAGKMDKSGEIKGFLEKDRNRNIVYVKENGTGDGKQIHTI
                     FRTLAVSESIRELGRQFSLLEAELVTGRSHQIRAHFAYLGHPIAGDVKYGDKTINEYF
                     RKRYGLKHQFLHAHKVAFEEVHEDISYLKGRSFHCDLDQTAAMTAASLFGTDWV"
     gene            17666..18589
                     /locus_tag="PKU88_08180"
     CDS             17666..18589
                     /locus_tag="PKU88_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPX69290.1"
                     /translation="MRKRMIQDPAAAIVDAAKSDNCVEDVLNMIEAPKIIGQGACVVI
                     TANMVDNKPASTGTVVHPDLLRKVIRYAKKFNPSRIVVAAGSGGAPTQQVFEEIGFNR
                     IIREEEIEFMDLNYGPYIELKLNHDIIPSIKINKLCEEMDVHISFTPIKMHKEATVSL
                     GIKNVALSWPPAEIHGMPKKKLGIHEDLHNFIAAMGEVVPIDITLLSGMNGMIWTGPS
                     DGKAVSSNLIVAGTDPVAADAVAARMMGLLPQAVHYLFELHRRGLGQADIKKINMKGV
                     PLVQAEEMFSMAALGESIAIDRPRILTFHGD"
     gene            complement(18586..18912)
                     /locus_tag="PKU88_08185"
     CDS             complement(18586..18912)
                     /locus_tag="PKU88_08185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPX69291.1"
                     /translation="MSYVPKQGDILWITLYPQLGHEQKGRRPGLVISNDTFVKMTGLI
                     LLCPITRTDNGFPLHLKLSDYNTCGYVMIEHVKSMDYNQSDVEYIETLRPEDLVEVLS
                     VLNACF"
     gene            complement(18916..19167)
                     /locus_tag="PKU88_08190"
     CDS             complement(18916..19167)
                     /locus_tag="PKU88_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPX69292.1"
                     /translation="MYISKVQKWGNSQGVRIPKYILDHADISEGDNVAISLEDNKIIL
                     FRPNKALKKYTLEELFKDYKGDYKPEEYDWGVPVGKEEW"
     gene            19661..19822
                     /locus_tag="PKU88_08195"
     CDS             19661..19822
                     /locus_tag="PKU88_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69293.1"
                     /translation="MDNNRVLLSNLKAYHKLEKLLKIQEALDSNKIIQKAKANDAKVE
                     SCTASNPII"
     gene            20022..20912
                     /locus_tag="PKU88_08200"
     CDS             20022..20912
                     /locus_tag="PKU88_08200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPX69294.1"
                     /translation="MQKKYPVRLSENRIFVDAYMSQQLGFSSRQIKKLFKDKKVCING
                     KTAYRDNKLKDGDIIEVDLSVEAREDIVPENIKLDIIYEDEYLLAVNKPPYMLVHPTP
                     NHPGGTLLNAAAYYFAGKGEEVSLRLLNRLDMNTSGIVVMPKNGLVHGRLDEMMRSGG
                     VRKFYTAVIEGIAAPEKGVIDRPIGKDESDPIKRKVKSDGQPAVTKYDTLKTGKSHSL
                     VRLELVTGRTHQIRVHLSFLGHPIVGDTLYGKKSCLIGRQALHASDMELAHPVTGNLL
                     KLHAEFPEDIRLLASRLELE"
     gene            20920..21810
                     /locus_tag="PKU88_08205"
     CDS             20920..21810
                     /locus_tag="PKU88_08205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPX69295.1"
                     /translation="MKSYNTYIIDKENDGITLGVYLRSRLMMSRNGLIKVKKSGSLKV
                     NGFKAHTDIIIKAGDKVEFELPDRNSDNILPEFMPLDIIFEDDYLIVINKEAGIPTHP
                     SRNHYMGTLANGLMYHWMEESRDITIRPVNRLDKNTSGLVIFAKSSHIQHLMSAEGYR
                     SNITKEYLAVVQGVVEADSGTIDAPIAREKLHSIRRVVREDGAEAVTHYSVVERHEDC
                     SLLKIVPETGRTHQIRVHMAHLGHHLLGDELYGGRLEKIKRHALHAYKIKMLHPIENR
                     MLDFTAQVPSDMEELIKKHF"
     gene            complement(21794..22174)
                     /locus_tag="PKU88_08210"
     CDS             complement(21794..22174)
                     /locus_tag="PKU88_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HPX69296.1"
                     /translation="MKPLIIIISLTILIVAGGSITLYLLNSESQRLDDSLSSLGEAIE
                     NGNWDNASKNLKEFNSKWDRTSSLWSMLVDHYEIDNIELLLSQLASYVKNKNRNDALS
                     SVSSLKTLIKHIPEKESLSLKNVF"
     gene            complement(22199..22885)
                     /locus_tag="PKU88_08215"
     CDS             complement(22199..22885)
                     /locus_tag="PKU88_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPX69297.1"
                     /translation="MLIVFVRTLILYILIIVVLRLMGKRQVGQLQPSELVVALIIADL
                     AAVPMSEVGIPLINGIIPIITLFIMEELLSYISMKSERARGLISGKPSILIERGTIIE
                     DELRRIRYNINDLLEQLRLKNFSNVEDVSYAILETSGQLSVIPKEEKKPVTLKDMNLI
                     AKTGHLPVTVIIDGRIISDNLYKIGLSNNWLADQLRKNSIKSSEDVFFAYLNPERKFI
                     YQLRSNSQKK"
     gene            complement(23095..23514)
                     /locus_tag="PKU88_08220"
     CDS             complement(23095..23514)
                     /locus_tag="PKU88_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPX69298.1"
                     /translation="MKVKQIMTTDVSTVTPNDTVTKAASIMKQLNVGSVPVTDGSRVV
                     GIVTDRDITLRGVAKSGDPNQKISEVMTTDVKYATPDMDVHAVADLMAENQVRRLPVI
                     DNDRLVGIVAIGDLAVENIFENEAGEALHNISMGVRH"
     gene            23795..24355
                     /locus_tag="PKU88_08225"
     CDS             23795..24355
                     /locus_tag="PKU88_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPX69299.1"
                     /translation="MNVEAGIIVRCKKYDKSAFTELFKMYEKYLYKLCYNYVQNEQDA
                     LDIAQEVYIKVFNSISSFDVKMPFHPWFRTIAVNTCLNFKRTIRYDSVSLNAGYEDDR
                     TIEEVVAASKDVESEVLDNELGHIIRENLECLRPKHRMVLILRYYEGLSYEEIAAVLK
                     EPLGTVKTDIYRARNILKEKLRNAMK"
     gene            24373..24807
                     /locus_tag="PKU88_08230"
     CDS             24373..24807
                     /locus_tag="PKU88_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69300.1"
                     /translation="MNCGFDREIIQRYADNTIDPLEFIFLKEHINYCGECRKELDMVM
                     TLENELYKFFDDDSEIRNPDMNIARLVDDCMYGLNKREKLKYTLTRSIKFGKKVADNS
                     LKFAGYIPGSKIICKGAKRTASYTGNLIKSLVKKEVKKLLAR"
     gene            24830..25486
                     /locus_tag="PKU88_08235"
     CDS             24830..25486
                     /locus_tag="PKU88_08235"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HPX69301.1"
                     /translation="MVVLLIIVKILLYLLLFLLGLVFLLLIIPVNYRGQVLTAEGFRA
                     QFAFGWAGKLFGISAEMEGENLDVTLRILDKRVCKLKSKGQEGKKEQSEEKTEKKEKE
                     KNVPRFREITDRTLINEILEYIKRVLNIVKPKYMHLYATYGFDDPSLTGMVCGAAAVV
                     KSMIPHARVGLYPDFTQEIIDLDLRAEGSMTVGSLAYQTIRTALKKPVRIILFRKKKS
                     "
     gene            25557..25946
                     /locus_tag="PKU88_08240"
     CDS             25557..25946
                     /locus_tag="PKU88_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPX69302.1"
                     /translation="MESKTPVNESLETLFSHLEKFLKTETVIGEPIVVGETTLIPIIT
                     VAFGCGGGGGGGKDEKGNDGMGTGVGVGAKISPDAVIVIKQDSVTMLPVKNRSNIEKL
                     VGMVPEIVKKIKLKKEEKSCCAEEKSE"
     gene            26294..26893
                     /locus_tag="PKU88_08245"
     CDS             26294..26893
                     /locus_tag="PKU88_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69303.1"
                     /translation="MAELELEIEKFSEIDGIGAEELKDMLIPKLGTTLSRETKGYNVY
                     VDLMQHKLNKLGYKDMEGKELKEDGVFGTNTEFAVNTFKETNKLWNHNEYRGKVGQTS
                     YDMLYSGKARRSPGYDDQGTGDDDISFKAVIYLNRSNRANLQGHAAIMLVTEDNRGLF
                     YSFTGDDTDVFKVLNGENVSGALLTVVDPETKNKHSFRM"
     gene            26926..27393
                     /locus_tag="PKU88_08250"
     CDS             26926..27393
                     /locus_tag="PKU88_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69304.1"
                     /translation="MLQFDGKEIKDDNYDRYIYMSINNEQGQKMYDRAEKLRSNTPDY
                     NLYEFNCNHMAQTILLEADLSFSKMRGNASDEKELLDAPYKIGVWDIISAYRKDREDL
                     TIPNAAYKHGVSIAEDNGWIIGETDWKHETIRTRRGSKEYQHEHNPLEYNPFR"
     gene            27415..28260
                     /locus_tag="PKU88_08255"
     CDS             27415..28260
                     /locus_tag="PKU88_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69305.1"
                     /translation="MRKWMIIISCIGLYLIMSGCSLNKSNLVGEIMLNSREGITAYSP
                     AENKFKSVLAGNFNNEVAINADKTKILGYKSIDENKGDYTVIFEYDMANDKFFSVMEY
                     DRSLGTGGKECIKYVPNSNKISFIANDKLYIFDRDKGEKTAILNEVDDQYSWDTNGEK
                     ILYADKDEKIYMYDMIKKEKQFICEGIHPVLSNNNEYIAYMAKDHMLVVREIKTKKEW
                     RGIDLLRDRRFVFSPDDKYLALAKQEYSIANNPYTIMYAVDYRKGKVTQLFKGDYSSY
                     GFDWK"
BASE COUNT         8953 a         5354 c         6283 g         7992 t
ORIGIN      
        1 aaaggtggta tcctttttac gctcgttttt ggtctcctta attttatcat tcacaccatc
       61 ccatttgaag cagggtttct gtcaaaaaat atcccaatat tgaattttgc ataattccat
      121 aggaaagata tgccatcccc ataagggatg tcccaaagta tacatactgc atcctgggaa
      181 ttttatttga aaagcaatat aaaacaagta acaccaagat atagggtagc attccataat
      241 aggctgataa tgagccatac atttctttat aggatggata tataacataa atggcaatgc
      301 ctccatttaa tttgattata aacagaaaga gacacactgt taatattagc ttggtaggaa
      361 aaggctctga ttcaaaatga atttcgatag gattatcaac ttttaagttg tatgatgccc
      421 aaaaacttaa agacagtacc cctgtcaata taaacagtat cgtattaaac gtaaaatatt
      481 ccaatccaat atttatcaaa taatagatga tattgccaac tataattaca agtgccatta
      541 ttttaatttt cttgtttata ggaatttgac gggtgtaata agaactccat gtaatgatat
      601 atataacact gcaaataccc attatgccca gagtaaaata ccaccaatga ggttttgaca
      661 gaaaaataat tagtgttcca gtcaagcata caaatatgca gagaatcata attgtcttta
      721 aatgattttc ctttggctta tatataaaaa aaacgaatag gaagagcacc ggtacaaatg
      781 tgtatattag actcagaggc tctacagctg tattggctgc agccgatatt gcccttaaaa
      841 caggcccatt atagggaaag gacaatagcc agcccagaaa cgcaccataa gatattacga
      901 tccaccaata gctttttttc attgtatatc tcccgacaaa tatctatata tccacatact
      961 actattcttt aaatattcag caaattcctt cataattgcg gataaaaaaa cagtcctttg
     1021 tggactgttt agaaaggtag cttttattta gcggattcac cattggtctg cgaagctgtc
     1081 tccgatttca gttttcctat ccaggtataa tcattcgctg tacttgactt gtagcgtaat
     1141 cccagaaaga gcagattccc gtttatttcc ttgatatcaa gtatcccata attttcatcc
     1201 caaccagatg taatcagatt gtatccagca tctttgccgc tcaaatccat ttcttttata
     1261 tatgcctgat acttttcatt aggatcaaac cttgacatca tccacttgtc tcgaggcttg
     1321 tattgtccaa aagctacaat gtccccatta cttaatgctt caattccata aattttaatt
     1381 tcataggggt attttttata gtctatatgg tttattccat aaaactcatt ctgccagata
     1441 cttttgaact gataatcata ttttgtaatc tgatatttca cattgcttcg gctgtaagtt
     1501 atgttgcccg atttatagct tgcatcttca actgtctgtg tcaacataac aaacccaccg
     1561 tcatttgtca ttgaaatgca gtttttagca gatgaattcc tcggattgtt caccaagaga
     1621 cctgcatcaa gcagcttttc acccaatata ttgccgttag aatcgattct tattaaataa
     1681 atctgattac cggacctccc caccagaatc agggaattat catgagcctt tacaatatcc
     1741 agtaaccatt ggcttgtgtc acttccaatt accttctccc atataagctt acccttaagg
     1801 tctatcttga aaaaccagac attgctggac catgcgtcag ctgccttaac tgcttttgag
     1861 cctccagcag taccaacgaa aatgtatccg tcctcgacct cagcccaact gtttatattg
     1921 cttgctggac cagcaccggg aagcggcagt ttcaaatcat cctgcaagca tatcgaaaac
     1981 aattgtttca tatttgcatc gattttgtct atgtatgatt tttccatagt atatttctta
     2041 gagcttggag gcattgatac tttagaggac tgacgttgtc ttataaaacc cccatcggag
     2101 gttggtctgt attccgaagt aaaatcagtg ttgatatagt cagctgtctc atcccatttg
     2161 cttatagcat caaagtcctt ggaagaattg aatctgtata ttttatgctg tgtcaatgtg
     2221 tttatagttt tcttgctgta gaggacagca ttattctggt tgtctgcata aatattaccc
     2281 ggaacgaaat gtccaagctt ctcaccttca aacagcactc tatcccagtc aatatgatga
     2341 ttttgttcta cggccgcata acagacaaag cttgatataa ttaaagccgc tattacaaaa
     2401 attagtaacc gtttcattct tatccccccc tttttataat gggaaagaag catgtatgtt
     2461 tatatagctt acacttacgg aactaccttc gtacttggcc agctgatatt tggctgtaat
     2521 atgttatcaa gatttgtaaa aggtcctcct gcaattgatg cttttacatt atttaatgga
     2581 taacctctgt tcgctactgt gccatttcct atttgtcctt catcattaaa gccccacgtc
     2641 catagcattc ctttcttgtc taaagccata ccatgatcat cccctgctgc tatatatgaa
     2701 acatcagata atccttttat ctgtattggt ttatttctat ccagaaaatg atgatacccg
     2761 tctataaaag tatacccatt cgccggttcc ccgttttctt ttttcggacc tgtgacttca
     2821 gtgaactgac ccaactgtcc atattgcccg cgcccccacg tccatactgt tccatcgttt
     2881 cttagcgcca ggctgaagtt ttgccctgct gaaatcatta caatattgtt caggtccata
     2941 acctgtttgg ggagatcgct atcattccag tctccaatac ctaactgacc ctgatcatta
     3001 cgtccccatg cccatactgt tccatcattt tttaaggcta agctaaagtt cagaccggct
     3061 gatgcagttt tcacatccgt aaagggctga ggcaattcat ctttagtatt atcctctaca
     3121 taaaggactt gtaccggagc attctggtct attccggtgt tgaaaacgcc aaacatacct
     3181 ccgctgcctc catttcccaa ctgacctttt tcattataac cccagctcca aagggttccg
     3241 tctgctttaa ctgcaaggtt atgccaatta ccggctgata tatgtacaat atctgatagt
     3301 gtttcaattt ttataggtgc ataccgtgtt gcgttagtac cgtcacccaa attgcccata
     3361 atgttattcc cccaggacca tacgcttcca tcctgcttga gtgccagatt gtattcatct
     3421 cctgccgata tttctataac atccttcaag ttggtcactt gtacaggctt gattatgcta
     3481 tagggcttag tcagagttgg tgatccctgt ccaacttgtc ctgctgcatt gttcccccat
     3541 gcccatactg ttttgtcttc ttttagacaa atactatgaa gcttccctgc cgaaactccg
     3601 attaccttat ctatttcaac agtctgacat gctgtgggaa cagatcggct ggttgttctt
     3661 cctaatccca attggttata gcggttgtat ccccatcccc atacagtacc atctttcctt
     3721 attataagat tatgcttatt tccggcagca ataggggtgt tgattagcga atctgtattt
     3781 tcagaagcaa aggaagtcat aaacactgcc tgaagaacaa tggaaacaat caaaagggtt
     3841 gcaactattt ttgtttttgt aaaataactt ctctttattt tcatttttct caaacctcat
     3901 ttcatttcgt attattacac tcattatact cctgacactt ctccttgtca tcaccccttt
     3961 ggggtgatac agatgatgat actgaaagtt ttctgtattg ttttctgtat tttcagactt
     4021 actgtcaagt aaacgttggc aacttacctt catacaaatt ttctgtaatt gctttttaaa
     4081 atttgatcgc atttattcca cggtcctatt ggcatgggta cccctgcacc ctctggacac
     4141 ctatttttag gcggaattaa cctatgtttt atgcggcttc cgccgccaag attttaccag
     4201 agggtgccac cccatatcaa taggctttcg cggcataaac gctatgctta tttttattac
     4261 ctatagacta aatcagtaaa atttctcata ttaaacatgc tgcatatagc ttttcttcca
     4321 ttggtaacag gataattcgc gaatggtact tgacccttat gtatgttttc atcttttcct
     4381 ttaccgtctt ttttaggcgc tttcgatgca cttagtatct gaattatctc actggttttt
     4441 tcttccggta atacaatctt tgaatacctt tcaacaacct catataatcg tttactcgcc
     4501 ttggcagcta ataacttccc taaattatta gttccttcga tactccagct tgctttactt
     4561 ggagaatatc aagccgctgg atatatcgcg tttgctgtcc tcacttggaa agcctggcgc
     4621 aagggtagat accgacaatc aaaatccata cagtgataaa ataattcaag ggtactacat
     4681 tcttttgcat actttgtttg ataaggcggt taagtgggaa atgatgtcag agaatgtatg
     4741 cgataaggtg gacaagcctt caataaagaa acacaaagcg gagttttatg atgaagagca
     4801 gataaacaat ctgcttgagt gcctgaatag tgaaggagaa gaattagaat taagccttag
     4861 caaacggaat cccaataata gaagtaagtc tgagcaagaa aagctggaac tgatggagga
     4921 aaggaaatac ctatactcta tgcacaaagc atatgtgtat ttagcattag caagtgcagg
     4981 aagacgcagc gaaatatgtg gattggattg gacgaatatc aattttgata caaattatat
     5041 cgtatcggat aagacactcc aatatacaaa agaggcaggg aagttttcaa ggccttatct
     5101 gaaaaacggc gatacatata aaggtatgta tctgccggag gtggtaattg atatattgga
     5161 ggaatattga gtggagcagc aacagtacaa ggaggtactg ggggacaagt ggattgaatc
     5221 aggcaaagtg tttacagcag tcaatggcga tttgatgagg gacatgggga cgtttctccg
     5281 agggacatgg ggacgtttct cctgtcccgc tttcagcggg acaggagaaa cgtccccatg
     5341 tccctcggag aaacgtcccc atgtccctcc ttttttattt cattgcaaag gtgttcctca
     5401 gttgtttggg agtaaaaatt cccatgaaga agagtatcaa aaagaagaac acaaggcctg
     5461 taatgcttga tactgtcatg ttgaagtttt gtaaagtcct tgccaccaga tacagattgc
     5521 agcacttgat aacgatgagc attccaacaa aagcgaaaag gggcttcata aatatattga
     5581 cataatctat atgtatattg ggaatacgct ttaagtctgc gagattccac aggcaaatca
     5641 acactgcact tgcacagaat ccgattacaa atccataaat tcctatagaa ggtatcggta
     5701 taaggaaata tgagcatatg acggatataa caatatgcag gattgagttt ttcagaacca
     5761 catgctgctt cccgacgccg ttcaaaacag caaataaagt gaactgccaa tattcaaaga
     5821 cacaaacgaa agacatccaa aaaagcatat tgccggcctc ggggcatttg aagaatatcc
     5881 tgcatagctc ccaggggaat acaataaata gggcagttga agcaaatgcg attgctgaag
     5941 tgattttgag ggagtcattg catttcttgt taagggccgt ccaattacct gcggcatagg
     6001 aggcggctat ggcagggaca agagtaatgc caagggaagc aacaataatg aaggggaaga
     6061 aaagcagggg cataaccata cctgttacct ggccgtatat tgataatgca cttgagttgc
     6121 tatagcctgc tgcacgaagc tgcatgggta tcaatatggc tgttatcata tccatgattg
     6181 ttgtcaggga accgcttagg gacaggggaa gagctgtttt cagtattctg gttatgatat
     6241 ggatagttga atcagtcttt gtatgaacta cagctgcttt tttgactttg tagcagaagt
     6301 acaaaagaat gaggctgata aactctcctg cagccatcgc tgccattacc ccggcgcaaa
     6361 cgtattctat tcccaatgga agcagataat atgtagtcat aagaagccct cccagccgga
     6421 tcattttttc aagtatgtca atcatggccg gaggcttgac atattgcagc ccgtaaaaat
     6481 atccttttaa cacggcactt aaggatatga agaatatggc aggagtaaat accatgattg
     6541 agaagaaggt cctgttgtcc ttgaggacat atttggccag atattcagag ttaaagaata
     6601 taaaggagca taaagccaag ctccatatcc ctattgtgat cccgcagatt ttaataatct
     6661 tgtacatatt cctgtaattg ctgccgggag cctgttcagc gatcagcttt gatactgccg
     6721 tagtgattcc tcccgaaacc aaagttataa aaagcaggta taaaggaagg accagctgat
     6781 atacccctac accttcagag cctattgccc tggtaagaaa taccctgtat ataaaggaca
     6841 gggatccggt aatgaggttt gttattgtca gaataaatgc cccttgtatg tatgtgagct
     6901 ttttcaatac ttatccactc cttataaaaa ccctataata aataaatatt acaagggcaa
     6961 gtacaatatt aacaaaaaaa aggcagccgg ttttggctgc cgagagagtg gatatgacca
     7021 agatttgctt tatttctttt ctatttctgc cagaattccg ttaatgaatt ttatggtcaa
     7081 ttcatcgttt actctaaggt cttcaaccct ttcagtttca tcatcaatta ttaccttgac
     7141 atctgaatcc actgtgtagg ttttggttgt gttatcaact tttacagtga ttttctttac
     7201 agtgctgatg cttattcccg tgagtgtgcc ggttacttct gttttatcat cttctgcaat
     7261 aacttctgtt actatatcgc cgacaatatc tactttcact ttcatgccca cttcaaaatc
     7321 cgaaatgctt gcatcgtcat catcgtttac tgttatttca gcactgcttg gtattgtgag
     7381 ggcaactgtt gtgccgtttg acttctttac tcttatttgt ttgccgcttg tattaattgc
     7441 gctgattatc ccttctgcgg taacaacctc atccaattct actatttttg tttcggacag
     7501 cttgttgttg ccctcgtcaa caaatctgac tccgtcaata ccatcgaaat tccttaactt
     7561 tacaatgctg tacgggtata ccgatttgct gccgcttgaa gatgcaggga cagttgtatc
     7621 tactacagct tttattgtat atttgtctcc gttttcaacc ttgtatacat catctatact
     7681 aatcttgtag tcctctgagt tctttttacc cattgttgct atcaggtaga taatgttgtc
     7741 atattcataa gccttgaagt ttttattgag cttcagatag tcaacctgat cctttaatac
     7801 ggctgacaaa tcatcgtaat caacttcggc aaaagacact ttggtttctt cagggtcttc
     7861 atcaaggtct tcaatttcta catcatcaag ttcatcatca tcttcatcta cgaaaatcac
     7921 ttcttcaatg tcatcaaagc tcttgaattt tactacactg tacgggtaag tgattgcctg
     7981 ggtgacattt gagccggatg acggttcatc cgtatcaact accgctctta ttgtatattc
     8041 atcgttatcc ccaatcctat ataccttttc tatttctata tcataaccgc cggttctctg
     8101 cctgcccatt gcagctacaa ggtaaatata gttatcatat tcatatgctg catagctttc
     8161 cttagacttc atgtcgtcaa tttcatcctt aagtactttt ggaaggctgc tgtcagatac
     8221 agcacggaag gagactgcaa tttcctcttc ctcatcatcg tcgccttctt cagcactctt
     8281 tacaacctca atgaatatta catctttgct gttgttcagc gtaagctgca gaatatctcc
     8341 ttttaccagg tcttctatag tttcaaagga accatccctg tacacctgta catcttcaag
     8401 cacatcgtaa gttacagtga ctccttttac cttgactgtg atttcattgt catcaacatc
     8461 tgcagagaca aatactgtgc tgttctttct gttgtcagtg tccggaaggt cgaacttgcc
     8521 ttcagccctg tcgagaagta cggccatttc agcccttgtc acaggctcca ttggcttgaa
     8581 catatttttt tcattgccaa tcataatgtt cagtttactt acaaggtaca cataacccac
     8641 tgatttatcc ggtacagcgc tgctgtcctt gaaatcaagg tcttcatcca tatgctttaa
     8701 agcttctttg gtttctccca ttgctctaat gatatacttt gcaatttcat gacgctttgc
     8761 aggttggttg ggattgaaat ccttcagttc ttcagggagc aggattcctt tttctattgc
     8821 catgaatata tagccatagc cccaattagg caatatacct ttatagaagg gatgaacctt
     8881 ttttcccatg ccttccgctt ctttgtcatc acctgtcagt ctcataacca ttgcaagagc
     8941 ctctacatga gtgacattgc tcctcggcgc atacttgccg ttgcctatgc ctccgattat
     9001 tccttttccg gccagctttt caattgcatt gagggcccag gtgatatcag cgcaatcatc
     9061 aaatctgtat tttttcactt cgttcttctt gattttggtt tgcccatatg cattaccttt
     9121 gccaggagat acaactttga ttgttatatt tcctcctgct gccggctttt tataatctgc
     9181 tgcaaccgta gtagttaagg aagagagtgt gaatactact gcaattccca gtgccgccaa
     9241 ctttttcttc aacataagat ttcctccttg tttctttttt cttttaatgg atttttgtga
     9301 ttaatataca attcgcaggt tggtcatgat ttttgggtgt aatttgaaaa aaacttatca
     9361 aatcatttta ttgacaacaa ttctgtctta tgatatcttt aaaaaaaaag ataaccttta
     9421 atttagtcct gtgaggctgg taaggttttt gatagtgtat ctggtgactg gaataacttc
     9481 atgcccacag gtgtgaggtt ttttttatgc cctgaatggg taacctttaa ataatagtcc
     9541 ggagagacta agaaggagga gaatacatga gtttaaaagg cagacatttg attgacccca
     9601 atgatttcag cctgtcggag ctggatgaga tttttgacct ggctgacaga atggcggatg
     9661 acagagattc atttgggaat atctgcaatg ggaaaattct cgggacactg ttttatgaac
     9721 caagtacaag aacaaggctg agctttgaat cggcaatgct gagaatggga ggaaaggtac
     9781 ttggtttttc tgatgcaggc acaagctcgg tatcaaaggg ggaaagcata gctgacacca
     9841 taagggtatt gagttcatac gcagatgttg tggtaatgag gcaccccaag gagggagccc
     9901 ctaagcttgc ttcggaatat tcactaattc ctgtaataaa cggaggcgac ggagggcatc
     9961 agcacccgac acagaccttg acggacctgc ttaccataag aagatataga ggaacattgg
    10021 agaatcagac aatagctttc tgcggggatc tcaaattcgg aaggacggtt cattcactga
    10081 taagcgcaat gtcgtactat aaaggatcaa aattcgtact tatatctcct gctgagctta
    10141 ggcttcccgg acacttgaag tcaagtctta ttgaaaagca taatgtgggt atttatgaga
    10201 cagaaaacct tgaagaagtg ataaaccagg ttgatgtgct gtatatgacg agaattcaaa
    10261 aagaaaggtt ctttaacgaa gcggattaca tgaggcttaa ggacagttat attctaagca
    10321 gaagcaagct taaggattcc agggaggata tgctggtaat gcatcctctg ccgagggtta
    10381 acgagataag ctatgatgtt gatgatgata agagggcagt atactttaaa caggcggaat
    10441 tcggaatata tgtgagaatg gcgctgatgg cgctcttatt gggggtgaat gtaaatgtta
    10501 accgtaaata gcattaattg cggtatagta atagaccata tagctgcggg caatggaata
    10561 aaaatctttg agaagcttaa gcttgatgag gtagatttcc cggtggtgct tctgattaat
    10621 gtaccaagca ggaagcttgg caggaaggat atcataaaaa tcgagaacaa gatagatgtg
    10681 gaccttacaa tgctgggatt gatagacccc aatataaccg taaacataat tgaaaacgat
    10741 aagatagtca aaaagatgaa caccattata ccggaagaag tgcagggatt gttcaaatgc
    10801 aagaatccca ggtgcgtcag caattttgac gagtatgtaa ccccaaagtt ccacctggct
    10861 aaagccaatg gaaaaattca ttacagatgc ggctattgtg aggaattgac aacctacagc
    10921 atataaaatt tgatacgagg tttcagctaa actttgaagc ataaagaatc ccagggtgca
    10981 catcaattcc cgggattctg ttttttagcc gggttacagt gaaaatagct gaaagggtta
    11041 tatcggaagt aattttatgg tataataaaa aaaagggggt gttattatga agatgccgga
    11101 attttccatc ggaatgtcaa aatctgttca aaaggtcata accagagaag acactgcatt
    11161 gaattttggc agcggagcat tgaagaatct gctggcaacg ccgacacttt ctgcgctgat
    11221 gattgaagcg acagtaaaaa ctattgaccc tctgcttcct gatcgctata ttacaatcgg
    11281 gaagagcatt gagattgagc atgagcatgc aaccaaagaa gggacaactg ttactgtgag
    11341 tgctgagctt gttgagatta acggaaatgt attgtccttt aaaattaccg cctttgatga
    11401 gatcggaagg atcggtacgg gctatcatgt cagacagata gtgaattatg atatcctgat
    11461 gaagagagtt gaagaaagat ttagtgtaat ggagggcaga ccttaaaatt ttcatataaa
    11521 aattaaaaat ttttgttgta atttttatcc cgctggaata taatagagtg taacaaaaaa
    11581 aataaatatg ctcaatcgat cgctgagtcc ctttcaaagg gaacggggga accaaaaaat
    11641 ggggtgaatc ctgaatagtc aggtagggct aaactctttc agtccgaatc cgtcagctaa
    11701 cctcgtaagc gtgggaagag gaggactggg tgtaatttga aatagttgca gccgtggttt
    11761 actccacggc tttttcaacc cccaaattac ctgttatcac ccgccttctt taaaaaaaag
    11821 gaggtattga aatgtcagta gcaaccaaat cgtacagtgt tgtcaatccg tatcatctca
    11881 aaaagcttct tagagtcaga aaagcagaaa taagtgatat caattcaata tacgatgttg
    11941 catcttcggt aggaacctcg gagaaaatct ccgaacaggg gtttcttgtg gatgattacc
    12001 agtcgaaacc taattattat aaaaagaaaa taggccattg gattgaaaac ctggagcgtt
    12061 tttatgttgc cgaatatgat aaggtatacg gattcatgat agcctataca aaagaggagt
    12121 ggctaaggga caatcccagc tggctccagg atgtatactg gaatccggaa tttgatccaa
    12181 gctggacgaa caagtttatt gtggttgaca aaactgccgt atttgcagat ttgacgggta
    12241 tgggaatcgg aagccttatt tacaagaggc tcctggagga cttaaggaat gaaggcatta
    12301 agcatatttt tgcagagacc ataatcagcc ctcagcccaa ttttgcttcc ctccagttca
    12361 ggaaaaagca gaaatacagc cttgcggggg tacgttacga gaagtacaat aatatccttt
    12421 atactgattt gatttaccat aaaagtgcgt ttgatgctct tacctgatta attgaaagga
    12481 ctaaaaaaag ggatatcgaa aactatttat tcaatagttt tgcgacatcc cttttttaaa
    12541 tattatctac cggacatgct tctttcagcc tgctcgatta atctcttcac cattgtcccg
    12601 cctactctgc ctgcgtccct tgccgaaatg tcaccattat atccttcttt taagttaaca
    12661 cctacttcgg atgctacttc gaatttcatc ctatcaagtg ctgccgttgc ctgaggtact
    12721 actttctgat tattcctagc catttatatc acctcctgat attatgtttt actgaaacga
    12781 gaataatatt agtgttatat aattgatacg attccaattt tagcttgcaa ttgaaataaa
    12841 gatatgatat aaaataatta aaagtcaaag aaagtcaaag tcaaaacgcg gaggttaaca
    12901 tcatggatat taacaaatta acacaaaaag cccaggaggc tgttatcgaa tcacagagcc
    12961 tggcaataaa gcacaatcat cagactgttg aaggagagca tttgcattac gcaatcattt
    13021 tgcagaatga aggattgatt cccaagctgc tttcaaaaat gaacgtgcct tttgattttt
    13081 attataagga tatttcagac ctccttgaca agattccgaa gatttacgga gagacagctt
    13141 ctagtcctta tgtttccaga aggtttgagc aattattctt aagggcggat gaagaagcaa
    13201 aaaaattcca ggatgaatat atcagcgttg aacatattta tctggcgctg attgatgaaa
    13261 caaatacacc ttcttcaaaa attataaaaa aatacggaat aaccagggaa aagttcttaa
    13321 gggaactttc cgccgtaaga agcaatcaga ggataaccgg ccaaaatccg gaggaaactt
    13381 atgaagcact gaaaaaattc ggccgtgacc tggtggaaga tgcgcgtaaa ggcaagcttg
    13441 acccggtgat tggcagggac aatgaaataa ggagaataat caggatacta tccagaagaa
    13501 caaagaacaa tcctgtgctt atcggagagc ccggagttgg gaaaacagct gcagtagaag
    13561 gcctggcgca gcggattatg aagggggatg tgcctgaggg gctgaagaac aagacgattt
    13621 tctccctgga tatgggtgcg cttatagccg gtgccaaata cagaggggaa ttcgaggaga
    13681 gactcaaggc agtccttaaa gaaatagaaa aatccaacgg cagaataatt atgttcatag
    13741 atgaaatcca taacatagtg ggcgcaggga aggcggaagg tgccatggat gcagggaacc
    13801 tgctgaagcc aatgcttgca aggggggaac tgcactgcat cggggctact acactggatg
    13861 aatacaggaa gcatatcgaa aaggacgcag ctcttgaaag gagattccag ccggtgctca
    13921 tagacgctcc ggacgtggag gataccatat caatactgag agggcttaag gagaagttcg
    13981 agatacatca cggggtgagg atacaggata atgctctgat atcggctgca gtactttcaa
    14041 acagatatat cagtgacagg ttcctgccgg acaaggccat tgaccttatt gatgaagccg
    14101 cagccatgct gcgtacggag attgacagca tgccaggtga gcttgatgag ataatgagaa
    14161 ggataatgca gctggagatt gaaagggaag cactgaaaaa agaaagagac aagcaatccg
    14221 aggaaagatt ggaggccttg aacagggagc tttcagagct gaagtccaaa ggcgattcca
    14281 tgaaggctca gtgggaattg gaaaaggaga acattaagaa tatccgcgag ataaagaaga
    14341 ggattgagga gacaaaggct gaaattgaga aggcggaaat gaaatacgac ctcaacaggg
    14401 ttgcggaatt aaaatacggc acgcttattg agcttgagaa gcagttaaat gctgaaaagg
    14461 caaaccttga aaaaatgcag ggcatggggc agcttctcaa ggaagaggtt acagaagagg
    14521 aaatatcaga tataatatca aaatggacag gtatacctgt aaagaaacta atggaaaatg
    14581 aaaaagaaaa acttctccgg ttggatgaca tactccacaa aagggttatc ggccaggacg
    14641 aggcagtatc tgccgtagcc gattcggtga tccgtgcaag aagcgggctc aaggatccga
    14701 agaggcctat cggttccttc atattcctcg ggcctacagg ggtagggaag acagaacttg
    14761 ccaagaccct tgcggaagcg ctgtttgaca gtgaggacaa cattatcagg atagatatgt
    14821 cggaatacat ggagaaacat acggttgcaa ggctgattgg agcacctccg ggatatgtgg
    14881 gctatgatga gggagggcag cttaccgagg cagtcagaag gaagccttat tcggtactgc
    14941 tgtttgatga aatagaaaag gcccatcatg atgtatttaa catattcctg cagatacttg
    15001 acgaaggaag gcttacggac agtcagggca ggactgtgga tttcaagaac acagtgataa
    15061 taatgacttc aaacatagga agcatgtatc ttttggaagg ggtggacaat gacggtgagc
    15121 tgaagcccga ggcagtagag gccgttaatg aggagcttaa acggcatttc aggcctgagt
    15181 tccttaacag ggtggatgat gtggtgtttt tcaagcccct gctgaaggaa cagataagaa
    15241 aaatcattga cctgatattt gaacaggtgg cggaaaggct taaggacagg aatataaaaa
    15301 tctcattaag cgaaaatgcg aaggactata taatcggcaa ttcctatgac ccggtatacg
    15361 gagcaaggcc tttaaaaagg ttcatacaga ggaaaatgga gacttcaata ggaagggctt
    15421 tgattgcagg ggagattaat gacgggaatg acatactgat tgacgcggat gaaaaggagc
    15481 tgaaattcaa gaagctttca tgaagaattt gctgaatcat atttgaaaca gtgcaaacct
    15541 gtgatatatt taaatataga gattaaagca cacagttatc atttgttaag gaggagatga
    15601 aatgggcaag acgtacgagc agataaatgt gggggataag ggaagcttcg agaagacaat
    15661 aagcgaaact gacatatata tgtatgcagg agtaacaggg gatatcaacc cggcgcatat
    15721 taatcatgaa gctatgaaga acaccaaatt cggggggagg atagcccacg ggatgctgac
    15781 ggcaggtttg gtatctgcag tactgggaat gcagcttccg ggccccggaa ccatatatct
    15841 ttcacagcag ctcaacttca aagcacctgt aaaaatagga gatactgtaa aagctgtggc
    15901 ggaggttgta gaaaagctgg agaagggcag agtaagatta aaaacgagct gctataacca
    15961 ggacggagtt gaagtgctgg acggcgaagc tctggtaata gcgccaagag aatagaagga
    16021 agggacttta tgcaatcagt tatcagaaac caaaaggatg ttgaaccggt aaaaaatcca
    16081 aagcacaacg aagtatacgg cagactgctt gctaaacagg aggaaaacgg caggctttcc
    16141 gcacatctga ctaatattaa gccgggggga gaaatccttc cccataccca tgaggtgctt
    16201 gaagtatttt atgtaattga aggggaaggc ttggcgctgg ttaacggtgt aaggcaaaag
    16261 gcggaaaaag gtacaattat tgctgccaat acaggcagcg aacatggaat aataaatacg
    16321 ggagaatcgg atatggtgtt gttctgcgta ttctctccgg gaattgcata aaagctgtgg
    16381 gccataagtt aatacttata gcccatattt gttattgtat agggaagtat agtttcctat
    16441 aaaatggggg atgcaagggg gagaatccct ctgccgttca aggagggtgc tcaggatgcg
    16501 gagctaacgc cttcgggaac tatagggtta cctagcgaaa aaaatgcgtc gtgagacgca
    16561 tttttataaa gctataattc cggaatgata gcgcaaaata taaggataac ataaattgca
    16621 gaggtgtaat atgttcgaaa tcagacttac tgaaaatgaa gcgggccaaa gagcagacag
    16681 gttcttaaga aaatacctga aggagtattc attaggggat atatacaagc ttttcaggac
    16741 aaacaaggtc aagctaaacg gcaagcgggt gaaggaaaac caaatgctta aagagggaga
    16801 cctgctgcag ctgtatgttg aaaagccgga aagggctgca aaaactgcga tacccttggt
    16861 aaaaccggtc ggaggcattg atttgatata tgaggatgcc aatataataa ttgctgataa
    16921 acctgcagga ctgctcaccc atcctgataa accgggggac accgatactc ttcttgacag
    16981 ggctttgtac catatatacg gtgaaaagga agctgacgaa tcccggacct tctcaccggc
    17041 agtgtgcaac aggctggaca gaaacaccag cggtattgtc ataatagcaa agaactacaa
    17101 gacacttaag gcagtaaacc attccatcag ggaaagagga ataagaaagc tctatctttg
    17161 cattgcagcc ggaaaaatgg ataaaagcgg cgagataaaa ggatttctgg aaaaagaccg
    17221 gaacaggaac attgtttatg ttaaagagaa cggaaccgga gatgggaagc agatacatac
    17281 aatattcagg acccttgccg tatcggaaag catcagggaa ttgggcaggc agttttcact
    17341 tctggaggca gagcttgtga ccggaaggtc ccatcagata agagcgcatt ttgcatacct
    17401 gggccatcct attgcaggtg atgtgaaata cggcgacaag acaataaatg aatacttcag
    17461 aaaaagatac ggcctgaagc atcaattcct gcatgcccac aaggttgctt ttgaagaagt
    17521 acacgaggat ataagctatc ttaaaggaag gagttttcat tgcgatttgg atcaaactgc
    17581 tgcaatgacg gctgcttcgc tgtttggaac tgattgggta taaaaaacac ctgccggggg
    17641 gaaaatttag gggaggtgtt tttttgtgcg caaaagaatg attcaggatc cggcggcagc
    17701 aatagtcgac gccgcaaaat cggataactg tgtggaagat gtactgaata tgatagaagc
    17761 tccaaaaata atcgggcagg gagcttgtgt ggtaataacc gcaaatatgg tggacaataa
    17821 gcctgcgagt acgggaaccg tcgttcatcc tgatttattg agaaaggtaa taagatatgc
    17881 caagaaattc aatccctcaa ggattgtagt cgctgccggc tccgggggtg ctcctacaca
    17941 gcaggttttt gaggaaatcg gatttaacag gataattcgg gaggaagaaa tagaattcat
    18001 ggacctgaat tacggacctt atatcgaact aaagctcaac catgatatta ttccatcaat
    18061 taaaataaac aagctgtgtg aggagatgga tgtacatatt tcctttaccc cgataaaaat
    18121 gcacaaggag gcaacagtat ccctgggaat aaaaaatgtg gccctttcat ggcctcccgc
    18181 cgaaatccac ggaatgccga aaaagaagct gggcattcat gaggatctgc ataatttcat
    18241 tgcggcaatg ggtgaggttg taccgattga tataacttta ctctcgggga tgaacggcat
    18301 gatatggacg ggccccagcg acggcaaggc tgtaagctct aatctcattg tagccggtac
    18361 agacccggtg gcggcggatg cagtggcggc gagaatgatg ggcttgctgc ctcaagcagt
    18421 gcattatctt tttgaattgc atagaagggg tttgggccag gctgatataa aaaaaataaa
    18481 tatgaaaggc gtgccgcttg tgcaggcgga agaaatgttc agcatggcag cactaggcga
    18541 aagcatagct atagacaggc cgaggatatt gacttttcac ggagattaaa agcaagcatt
    18601 taatacagat aatacttcaa ctaaatcttc cggcctcaat gtttctatat actcaacatc
    18661 gctttgatta taatccattg atttaacatg ctctatcata acgtacccgc aagtattgta
    18721 atctgaaagt ttaagatgca aaggaaaacc attatcagtc cttgttatag gacagagtaa
    18781 tatcaatccg gtcattttta cgaaagtatc attgctgata accaaacccg gcctgcgccc
    18841 cttttgttca tggccaagct gcgggtacag tgttatccaa agtatatcgc cctgttttgg
    18901 cacataggac atttattacc actcctcttt gccgactggt acaccccagt catactcttc
    18961 cggtttataa tcacctttat agtccttaaa taattcttcc aaggtgtatt tttttaatgc
    19021 cttattgggt ctgaataaaa ttattttatt gtcctccagg gatattgcta cattgtcacc
    19081 ctcagaaata tcagcatgat ccaatatata ttttggtatc ctgactcctt ggctattacc
    19141 ccatttttgg actttagaaa tatacattat aaatcactcc gtaattttaa attatattat
    19201 ttatattatc tgccatgcac aatcatataa tccctccttt tatcatgtat atataaagta
    19261 tatactttta aaaataatat gtcaatataa aaagcaaaat atagaaccat attaacctag
    19321 ctcaaatgct taaatagaac acactcctta ttaaacctat aaggaaaact gccgaatacg
    19381 gaggagttcc ggtaaagttt ataggctatc tctgataaaa gcggataggt atggatagga
    19441 atgtttaatc gcattttggc agagttcctt tgcaagaaat tgtaaaagaa ataggttata
    19501 atagaattaa gataaacgtc gacgttattt atatgagaaa taagttacga attcaggttg
    19561 tgtttgaaat agctttatat atcatttgga ggtgttgatc aagagagaaa gcaaaaattt
    19621 attattatca caaccaaatt tgaaagaaag aggtggaatt atggataata atagagtatt
    19681 actttcgaac ctgaaagcct atcataagtt ggagaagcta ttgaagattc aggaagcatt
    19741 ggacagcaac aagataatac aaaaagcaaa agccaatgat gcaaaagttg aaagttgtac
    19801 ggcatcaaac ccgataatct agaacgaaaa aatcgacgca agtcgatttt tttacatggg
    19861 gcatggatgg gacgtggggg ggacacgggg acgtttctcc tgtcccgctt tcagtgggac
    19921 aggagaaacg tccccgtgtc ccactcacgt cccattattc gtataatatg ctataattat
    19981 taggaacata agtaaataat aaagcgtagc ggggcgatgg catgcaaaaa aaatacccgg
    20041 taaggctcag tgaaaacaga atatttgtcg acgcttatat gtcgcagcag cttggttttt
    20101 cctccaggca gataaagaag ctgttcaagg acaaaaaggt atgtattaac ggaaaaacgg
    20161 catacaggga taacaagctg aaggatggag atattattga ggtggatttg tccgtagagg
    20221 cgagagagga tatagttccg gaaaacatta agcttgatat tatttatgaa gacgaatacc
    20281 tcttggcagt caacaagcct ccttatatgc tggtgcaccc tacacccaac caccccgggg
    20341 gaaccctgct taatgcagcc gcttattatt ttgcagggaa gggggaggaa gtatcgctgc
    20401 gccttctgaa caggctggat atgaatacct ccggaattgt tgtgatgcca aagaacgggc
    20461 tggtacacgg caggcttgat gaaatgatga ggtcaggggg agtaaggaaa ttctacacag
    20521 cagttattga gggaatagcc gcacctgaaa aaggggtcat tgacagaccc atcggaaagg
    20581 acgaatcgga tcccataaag cgaaaggtaa aaagtgacgg gcagccggca gttaccaagt
    20641 atgatacgtt aaaaacaggc aagagccatt ccctggtgcg cctggagctt gtaacgggca
    20701 gaactcacca aataagggta catctcagct ttctggggca tcctattgta ggagacactc
    20761 tgtatggaaa aaaaagctgt cttatcggca ggcaggcatt gcatgcatcg gacatggagc
    20821 tggcccatcc tgtaacgggc aatttgctca agcttcatgc agaattcccg gaggatatcc
    20881 ggttgcttgc atcaaggctt gaattggagt gagaagaagt tgaaaagcta taatacatat
    20941 ataatcgaca aggagaatga cggcattaca ctgggggttt accttagaag caggcttatg
    21001 atgtcaagga acggactgat aaaggtgaaa aagtccggtt ccctgaaggt gaacggattc
    21061 aaagcacaca cagacatcat tataaaagcc ggagacaagg tggaatttga acttcctgac
    21121 cggaactccg acaacatcct gcctgaattc atgcccctgg atataatatt tgaagacgat
    21181 tatttaattg tgataaacaa ggaagcaggc atacccaccc acccctcccg gaatcattat
    21241 atgggcaccc tggcaaacgg cctcatgtac cactggatgg aagagagcag ggatataacc
    21301 ataaggccgg ttaacaggct tgataaaaat acctccggcc ttgttatttt cgcaaagagt
    21361 tctcatatcc agcatctcat gtccgcagaa ggttacagga gcaatataac aaaagagtat
    21421 ctggctgttg tacagggcgt ggttgaggca gattcaggca caatagacgc acctatagca
    21481 agggaaaagc ttcattcgat acggagggtt gtgagagagg acggagccga agcagttaca
    21541 cattacagcg tagttgaaag gcatgaggac tgcagcctcc ttaaaattgt accggagacg
    21601 ggacggacac accagataag agtacatatg gctcatctgg gacatcatct ccttggggat
    21661 gaactctacg gaggaagact ggaaaaaata aaaaggcacg cactgcatgc ctataaaata
    21721 aaaatgcttc atcccataga aaacaggatg ctggatttta cagcccaggt cccttcggat
    21781 atggaagaac ttattaaaaa acatttttaa ggcttaagga ctccttttca ggtatgtgtt
    21841 tgataagggt ttttaaagag gaaacacttg acagggcatc atttctattc ttgtttttca
    21901 cataggaagc aagctgtgag agaagcagtt ctatattatc aatttcatag tggtccacaa
    21961 gcattgacca taatgaactt gtcctgtccc atttgctgtt aaattctttg aggttttttg
    22021 aagcattatc ccaattccca ttctcaatgg cttccccaag ggacgaaaga ctgtcgtcaa
    22081 gcctttggga ttcggaattc aataaataca gtgttattga gcctccggca acaatcagaa
    22141 tagtaagtga aataattatt atcaagggtt tcaattcgtc cgccgcccct ttctgcaatc
    22201 attttttctg cgaattacta cggagctggt atataaactt cctctccggg tttagatatg
    22261 caaagaacac atcttcagat gatttaatac tgtttttcct gagctgatct gcaagccaat
    22321 tattgctcag gccgattttg tacaggttat cggagattat ccgcccgtca attataacgg
    22381 tgacaggcag gtgcccggtt ttggctatta aattcatgtc cttcagtgtg acaggttttt
    22441 tttcctcctt cggaattacg cttagctgcc cgctggtttc caatatggca tagctgacat
    22501 cttcaacatt ggagaagttt tttaacctta attgctccaa aagatcattg atgttatatc
    22561 taatacgtct tagctcatct tcgatgattg ttccccgctc aataagtatg ctgggcttgc
    22621 cgcttatcag gcccctcgct ctttcacttt tcatggatat gtaggataaa agctcttcca
    22681 tgatgaaaag agtgattata ggtattattc cgttaatcaa aggaataccc acttcagaca
    22741 tgggaactgc cgccaagtct gcaattatca aagctactac cagctctgaa ggctgaagct
    22801 gccctacctg cctttttccc atcagccgta atactacaat tatcaatata tatagaatca
    22861 gcgttctgac aaaaactata agcaacaggc acacctctca ggtataaagg ttgatattag
    22921 aataattccc atattggcag agaaatatta atatataaga aggtttaata ttaaagaaat
    22981 aagtggtaat attatactag caacttatta caaaagagct gcatgcagca atcagctgat
    23041 gcaggatatc agagagaaag aaatctccgg aaggccggag attaaagaaa attattaatg
    23101 ccttacaccc attgatatgt tatgaagtgc ttcaccggct tcgttctcaa atatgttttc
    23161 tactgccagg tctccgattg ctacgatacc gacaagccta tcattatcta tgaccggaag
    23221 ccttcttacc tggttttctg ccattaaatc ggcaacagcg tgaacatcca tgtccggggt
    23281 ggcatatttg acatcagtgg tcattacttc tgaaattttt tggtttgggt ctccgctttt
    23341 tgcgaccccg cgaagtgtaa tatctctgtc agttacaata ccgactactc tgctgccgtc
    23401 agttacaggt accgagccta catttagctg cttcataatt gatgcggctt tggtaactgt
    23461 atcatttggc gtaacagtgc tgacatcggt ggtcataatt tgtttaacct tcacaagcat
    23521 catcctttca taatgagtta tttagatgtt tcgcagttaa catcttatgc tgaattaaat
    23581 agtgcgaaac attttccttg atgcatatat cgtgaaaagt acattacttt aatacaaaat
    23641 tagacatatc tactgatttt gtaatgatga atctttttca cgatatatat agctttacct
    23701 ttttatatat ttttagcagt taaataaaca gaaaaaaaat gaaacaattt atggtttttt
    23761 tgtgtaatat actgtatggc ctggaggtga tgctgtgaat gtagaagccg gcattattgt
    23821 taggtgcaag aagtatgaca agtctgcctt cacagagctt ttcaaaatgt atgaaaaata
    23881 tctgtataag ctttgctata attatgtgca gaatgagcag gatgctttgg atattgccca
    23941 ggaggtttac ataaaggttt ttaacagtat atcaagcttt gatgtcaaaa tgccctttca
    24001 cccatggttc agaacaattg cggtaaatac atgcctcaac ttcaaaagga ctatcagata
    24061 tgattcggta tcgcttaatg cagggtatga agatgacaga acaattgaag aggtagttgc
    24121 cgcttcgaag gatgtggaat ccgaagtctt ggataatgaa ttggggcata ttataagaga
    24181 aaatctggag tgccttaggc caaaacacag gatggtattg atcctcagat attatgaagg
    24241 attaagctac gaggagattg cagcagtgct gaaggagcct ctcggcactg tcaaaacgga
    24301 tatttacagg gcgagaaaca ttttgaagga aaaactaagg aatgcaatga aatgaagctt
    24361 ggaggtgcgg aaatgaactg tggatttgac agggaaatca tacagagata tgccgataac
    24421 accattgacc cgctggaatt tatttttctt aaagagcata ttaactactg cggtgagtgc
    24481 aggaaggaac ttgatatggt catgactctg gaaaatgagc tgtataagtt ctttgatgat
    24541 gattcggaaa taagaaaccc agatatgaat attgcgaggc ttgttgatga ctgcatgtat
    24601 gggctgaata agagggagaa gcttaaatat acactgaccc ggagtatcaa gttcggaaag
    24661 aaagtggcgg ataattcttt gaagttcgca ggatacattc ccggaagcaa aatcatatgc
    24721 aaaggggcca aaaggactgc ttcatacacg gggaatctta taaaatccct tgtaaaaaaa
    24781 gaagtcaaga agctcttggc taggtaatta ttaaatgggg gtacacccga tggttgtatt
    24841 attgataatt gttaaaatat tgctgtattt gctgcttttc ctattggggc tggttttcct
    24901 gctgcttatc atacctgtaa attacagagg acaggttctg actgctgagg ggttcagggc
    24961 gcagtttgcc tttggctggg caggaaagct gtttgggata agcgctgaaa tggaagggga
    25021 aaatttagat gtaaccctcc ggatactgga taaaagagtt tgcaagctga aaagcaaggg
    25081 gcaggagggg aaaaaggagc aatccgagga gaaaacagaa aaaaaagaga aagaaaaaaa
    25141 tgtacctcga ttcagggaga ttaccgacag gacattgata aatgagattc ttgaatatat
    25201 aaagagggtg ctgaatatag taaagccgaa gtatatgcat ttatacgcaa cctatggctt
    25261 tgatgatccc tcgcttacgg gaatggtgtg cggtgctgcg gcagtagtaa agtctatgat
    25321 tcctcatgca agagtagggt tatatcctga ctttacccag gaaataatag atttggattt
    25381 gcgtgctgaa ggcagcatga cagtaggaag cctggcttat cagacgataa gaactgcttt
    25441 gaaaaagccc gtaaggataa tattgttcag aaagaaaaaa agttgaaact tttttaatat
    25501 ataagtgtaa ataataatgt aaacaattat gaaaacaatt ataggaggat ataataatgg
    25561 aaagtaaaac accggtaaat gaaagcctgg aaactttatt cagccatctg gaaaaattcc
    25621 tgaaaacaga aacagtcatc ggagagccga tagttgtcgg agaaacaacg ctaatcccga
    25681 ttattacagt ggcctttggc tgcggcggcg gaggcggcgg cggaaaagat gaaaagggca
    25741 acgacggaat gggaacaggt gtgggagttg gcgccaaaat atccccggat gcagtaatag
    25801 tcatcaaaca agactctgtc acaatgcttc ccgtaaagaa caggtcaaac attgaaaagc
    25861 ttgtcggcat ggttcccgag atagtaaaga aaatcaagct caagaaggaa gaaaaaagct
    25921 gctgtgcaga agaaaaaagc gaataattgt gaggctgata acgtcaacca tcgattttgg
    25981 ttggcgtttt ttttatgctg cttgttcaca cttttttcat taaggtcaga atatatcgaa
    26041 tatagctgcc agcacgacag atagacagtt attgcagaag attggtcata ctggtaagaa
    26101 atagataaat gtcagatgca aagatcctat atgtgtcagg cattgaacaa aagacatatg
    26161 aggtaaatat ttacagcatg tacaccttct gttagaattt atacagaagg taaatttttc
    26221 gatattatta aaccaccttc aggtatgaca gcaacggaaa caaaagagca aggcagaaca
    26281 gctgaagaag cgaatggctg agctggaatt ggaaatagag aaattcagcg aaatagacgg
    26341 aataggggcc gaagaactta aggacatgct tatcccgaag cttggcacta cattatccag
    26401 agaaacaaaa gggtataacg tgtacgtcga cttgatgcag cataagctga acaagcttgg
    26461 ctataaggat atggaaggca aggagcttaa ggaggacggc gtattcggca cgaacacaga
    26521 attcgcagta aacacattca aagaaacgaa caagctgtgg aaccacaatg aatacagagg
    26581 gaaggtcgga cagacaagct acgacatgct gtacagcggt aaagccagaa ggtcaccggg
    26641 gtatgatgat caggggacgg gtgatgatga tatctctttt aaagctgtta tttatctaaa
    26701 taggagtaac agagcgaatt tacaaggaca tgctgctata atgcttgtaa ctgaggataa
    26761 tagaggcttg ttttatagct ttactggaga tgacacagat gtttttaaag tacttaatgg
    26821 cgaaaacgtt agtggtgcat tgttaaccgt tgtagaccca gaaacaaaaa acaagcattc
    26881 attccggatg taaatgaatt tctgaagact ggcaatatta aaaatgtgct gcagtttgat
    26941 gggaaggaaa taaaagatga taactatgac agatatatct acatgtcaat taataatgaa
    27001 caaggccaga aaatgtatga tagagctgaa aagctaagga gtaatacacc tgactataac
    27061 ctttatgagt ttaattgtaa ccatatggcg caaacgattt tgttagaagc tgatctgagt
    27121 ttttcaaaaa tgagaggaaa tgcaagtgat gaaaaagaat tgctagatgc accatataag
    27181 ataggtgttt gggacataat ttctgcatat agaaaggata gggaagattt aacaatcccg
    27241 aatgctgcat ataaacatgg agtgtcgata gcagaagaca atggatggat tataggagaa
    27301 acggattgga aacatgagac aattagaacg agaagagggt caaaggagta tcagcatgaa
    27361 cataaccctc tagagtataa tccattcaga taattgtgag aggtgatgtt ttttatgaga
    27421 aaatggatga ttattatctc atgtattggt ttatatttaa taatgtcagg ctgtagctta
    27481 aataagagca atcttgtagg agaaattatg ctgaatagta gagaggggat tactgcttat
    27541 tctccagcgg agaataagtt taagagtgta ttagcaggta atttcaataa tgaagtagcc
    27601 attaatgctg acaagactaa aatactgggc tataaatcga tagatgagaa caaaggagat
    27661 tatacagtta ttttcgagta cgacatggct aatgataagt tttttagcgt tatggaatat
    27721 gatagatctt taggcactgg tggtaaggag tgtattaagt atgtacccaa ttcaaataaa
    27781 ataagtttca ttgcaaatga taaattgtat attttcgata gggacaaagg agaaaagact
    27841 gccatactaa atgaagttga tgatcaatac tcgtgggaca ctaatggaga aaagatatta
    27901 tacgcagata aggacgaaaa aatatatatg tacgatatga ttaaaaaaga aaagcagttt
    27961 atatgtgaag gaatacaccc tgttctttcc aataataatg agtatatagc ttatatggct
    28021 aaagatcaca tgctagttgt gcgtgaaatt aaaactaaaa aggagtggcg aggcattgat
    28081 ttattgcgcg atcgaaggtt tgttttttca cctgatgata agtatcttgc attagcaaaa
    28141 caggaatata gcatagcaaa taatccttat acaataatgt atgcagttga ttatagaaaa
    28201 ggaaaagtaa ctcagctatt taaaggagat tattcatctt atgggttcga ttggaaatag
    28261 ttaaagagaa tatgtgccgg gcagtgcgtc cgagcaatgt cggaacatat agtgggtggg
    28321 aagcccatcg ggtaaggtta accagccgct cgtagtcagc cttggagcgc agcaggtaac
    28381 tgctgcgttt aagcgtaggc agacaagata gcaggccgaa agccgaaagg ttgaagtgat
    28441 tgagccacga aatactaaat aggaaggctg atgccgttgc ctgtgcagaa agcaaagaat
    28501 atgtgccagg cacgacagat agacagttat tgacagagat taatcctttt ggtattatat
    28561 gggtataatt accaggagga tt
//
LOCUS       DAOOAB010000033        27999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2220_length_27999_cov_14.551031, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000033 DAOOAB010000000
VERSION     DAOOAB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 27999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27999
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_27999_cov_14.551031"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..673)
                     /locus_tag="PKU88_08260"
     CDS             complement(23..673)
                     /locus_tag="PKU88_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPX69306.1"
                     /translation="MKKVELKADNRTGSGSKQSKKLKKENCIPAILYVKGSAGRLLKI
                     DESELNNIIAKNGENVVVKLRIDGTEIPAVIKEIQRDHLQQHLIHVDFQPVTLHEIIH
                     AEVPILVVNGEKIERSGWVINKQMTELEIEGEVEKIPPAITVDAAKYKVGQVLRVADL
                     EISEELSIVNEKNEVILSILPTKDRPIDLVFNRVEPELVTTEKRDKKKEKERPDVQ"
     gene            complement(876..1415)
                     /locus_tag="PKU88_08265"
     CDS             complement(876..1415)
                     /locus_tag="PKU88_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HPX69307.1"
                     /translation="MYFTEEGKKNTEKVAELVIKAARENNIAHIVVASNTGATARHFI
                     GCGMEITCVTHAYGYGQPGVNEMNRNDREELSALGVKVLTTTHVLSGAERSLSAKFGG
                     INPVEIIANSLRMLGQGVKVCVEISVMALDAGLIPYGEPVIAVGGTGRGADTAVIIKP
                     AHAKDILNTRIQEIICKPR"
     gene            complement(1569..2453)
                     /locus_tag="PKU88_08270"
     CDS             complement(1569..2453)
                     /locus_tag="PKU88_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX69308.1"
                     /translation="MFRFRKVSQADKSKVLEISSKIWEGHDFIQHVFDHWVSDAEGEF
                     TIGEKDGDVIGFAKYTKHSDSEAWFEGIRADEKYTGQGFGKAITQYFISRAKAEAVDT
                     IRLCTETASKECIRIVEGLGFKKDGYFTYGYKELGKDTKEPNVSKTVISIMSTATAWD
                     LITRGNSYYMSNGYISYGWVFKKATYDLTDKLVKDKCVYGVLQDGKVNSVMVLTEDSH
                     MDGGLNIGFIDGDMGSMKLLVDFAEQQAINRGMKYCEIMAPIDERIINVLEMSNFKFL
                     SEKYREVNVFVYFMKILG"
     gene            complement(2470..2721)
                     /locus_tag="PKU88_08275"
     CDS             complement(2470..2721)
                     /locus_tag="PKU88_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69309.1"
                     /translation="MNYIRVIRISGSFFAREFIKPEKARKKVQYREVDEKTVAEQFLK
                     GDATVEVIFEDSDRESIKLHLESDPSLIKKYLGDRFISY"
     gene            complement(2764..4806)
                     /locus_tag="PKU88_08280"
     CDS             complement(2764..4806)
                     /locus_tag="PKU88_08280"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPX69310.1"
                     /translation="MESYLQGLNKEQLTAAKHGEGPCMVLAGPGTGKTTVITSRVINL
                     IKCKSVPPENILVVTFSRAAANEMKERYGRLLGNRADEKVSFGTFHSIFYKLLRQYKG
                     YKLEDLIDENHKFNIIRNFVRRMESGFTEDEEQVRDIIGDMEYIISTMSEAEDFKPGS
                     CSSSQLKGIMKEYSEYKQKSGKYDYDDILFDCFHMLRENSGILKEVRERFKYILVDEF
                     QDINALQFEAIKLIAEPLNNIFIVGDDDQSIYGFRGAAPDILLNFERLYSGCSKIYLK
                     TNYRSTSNILTSALSVINTNNNRYSKDLEAVNGQGSLPVVFEAEDFEQEARIIASRIG
                     QMIKNGREYSDFAVIYRTNLQSRSVIDAFLDCRIPFITFDGMISIYNHWIYKDIISYL
                     KLGTGLGDTRDFIRIMNKPKRYISRDMMEKAENCGGDFIQGIIGQGSLNRLQINGVFG
                     LKNDLKRLGGMNPLNAVRYIRNVMGYDEYLKEYAASKGISTKGLSEIIEEIESSAGGF
                     KSILNYMQHTEDVVEKLGQKEYTNSGHNNVKLLTMHKAKGLEFETIFICGTVEGLTPY
                     IKDDSALRTDPEEERRLFYVAMTRAKRELYIAVPKRRYGKAVRPSRFIEEFQKSIDYS
                     SQVHAGQKVYHKIYYEGVVKEVIGKKGGTRIRVDFGGSIKELNLETCLNNEIIRLM"
     gene            complement(4907..5479)
                     /locus_tag="PKU88_08285"
     CDS             complement(4907..5479)
                     /locus_tag="PKU88_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPX69311.1"
                     /translation="MSDVKNILLVGVGGQGTILVSRILTAGLIKAGYDVKMSEVHGMA
                     QRGGSVTTQVRYGKKVYSPIIGKGQADILVSFEKMEAVRWIEYLKPDGVVVVNNYEIP
                     SMPIASGKEEYPRGLIEALQGSFKTVVIDAGKIAEELGNIKTQNIVLLGALVKALKMN
                     EIDWEEIIRETINPKLVDINIKAYNKGKEL"
     gene            complement(5481..7232)
                     /gene="iorA"
                     /locus_tag="PKU88_08290"
     CDS             complement(5481..7232)
                     /gene="iorA"
                     /locus_tag="PKU88_08290"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPX69312.1"
                     /translation="MKQLMSGNEAIARGAYEAGVKVAAAYPGTPSTEILENIVEYKDD
                     IYCEWAPNEKVATEVAIGASIAGVRSISAMKHVGLNVAADPIFTYAYLGVNAGYVLIT
                     ADDPSMHSSQNEQDNRYYAKFAKIPMVEPSDSQESKDFMAEAFKISEQFDTPVLYRVT
                     TRVCHSKSMVEFGERIEVEPKSYERNAAKYVATPANAKIARIKVEERMKSLEEYAYSS
                     PLNKIEYNKDTKIGIITAGISYQYVKEVFGDNASYLKLGFTFPLPMKLIKEFASKFET
                     VYVVEELDPFMEEQIKAEGVKCIGKELIPPMYELNPDIVAKALLNKENETKKVDAKVV
                     GRPPVLCPGCPHRGFFYTMSKKKNTVVTSDIGCYTLGGSPPLNAVDTCICMGAGFSAG
                     MGMAKAFEASGRDNKVFGVVGDSTFFHSGITGAIDIVYNKGKMVPVVLDNRITGMTGH
                     QENPGTGKTLMGEDAVQIDPVEVLRAIGYKTVKTVDPCDLKAMDEVVTEAVNSEEPYA
                     IVTKRPCVLIKGLPPFKTKCRVDRDKCKKCKMCLKVGCPAVFFKDGTSYIDQSMCVGC
                     EVCMQVCKFDAIEKVGE"
     gene            complement(7253..8155)
                     /locus_tag="PKU88_08295"
     CDS             complement(7253..8155)
                     /locus_tag="PKU88_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPX69313.1"
                     /translation="MIKNFNELLETAKSQKIMKIVIAAAQDEDVIKAVSQAKEIGIAE
                     PVLVGNKAKILEIMNELGIESSEFEIIDEPDLIQAARKSVKLVREGKGDFLMKGILQT
                     ADIMRAVLDKEEGLRTDNLISHVMVYEVPSYNKLLYLTDGGMNVAPDLSQKVQILENA
                     VKVCKAMKMKKIYASCIAGAETVNPKIPATVDAKAIEDMKDKWEEQGVVVQGPVALDL
                     AISSEACAHKGFKGEGGGKADILLVPYYEVGNAMGKAMTYFAGAKSAGVIMGAKVPIV
                     LVSRADSSESKLLSIALGSVVANN"
     gene            complement(8401..9012)
                     /locus_tag="PKU88_08300"
     CDS             complement(8401..9012)
                     /locus_tag="PKU88_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPX69314.1"
                     /translation="MNESVKKTIEALEKHNMKASYFDDSDTAVSYLMGKIGDGATVGI
                     GGSMTVYSLGIPQKLIERGNRVYFHWLESTPEGMDYARDKAQKADVYLSGTNALTEDG
                     KLVNIDGLGNRVASMIFGPKKVFVLCGVNKITKDLDAALKRIKENTYKNARRLKLDTP
                     CAHTEKCNDCNSPQRMCSVTTIIERRPYKTEFEVIIIGKELGY"
     gene            complement(9065..10498)
                     /gene="glnA"
                     /locus_tag="PKU88_08305"
     CDS             complement(9065..10498)
                     /gene="glnA"
                     /locus_tag="PKU88_08305"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPX69315.1"
                     /translation="MFKNLTEVKEFCNSKGIKIVDFKITDLAGRWHHLSIPVTRLNEK
                     VLEEGIGFDGSNYGFKTVEKSDMVFIPDLNTAFVDPFCEIPTLSMIGDIFALEGGIRR
                     YEGDPRYIAEKAEKFMASTGIADENIIGPEFEFYLFDQIAYESRPEHQEVAIDSAQAE
                     WNTNDQYEKNLGYKVRHKGGYHLALPYDLTNDLRSKMTVMLEEFGMPIKYHHHEVGGP
                     AQVELEVEPGKLKDMADKTYLLKYVVKNIAIQNGKSATFMPKPIFGEAGSGMHVHMWL
                     YKDGKPLFYDKDGYSGLSQTALYFIGGLLKHSPALLGFTSPSTNSYKRLVPGYEAPVS
                     ICFATANRSAIVRIPGYGNTPDSKRFEFRSSDATCNPYLAYSALLMAGLDGIINKIDP
                     VKEGFGPYDVNIFELDDEEKKKIKGLPKSLEEAMEALRQDHEFLLKGGVFTKSLIETW
                     IKNKIEKEHKKISIIPHPAEFELYYDL"
     gene            complement(10595..11485)
                     /locus_tag="PKU88_08310"
     CDS             complement(10595..11485)
                     /locus_tag="PKU88_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPX69316.1"
                     /translation="MSAKGKIITAVVVAVLLIAAAIYGFTYNFLSKISVEEGAEGYPV
                     PEKRVNVVVMGVAKGLADTIMLASFDPEDGALDVLSIPRDTYIERKGHKISANNKINS
                     SYGRGGADNVAESVVSLTGLDVHYFAEVDYEAVKELVDAMGGIKVTVPMDMNYDDPVD
                     DLHIHFKKGEVVSKGEDILKLLRYRKNNKGGGYKEGDLGRVKMQQEIVRLGIDKVMRG
                     NIAANFLKLQEPVKEYVKTNMPPKQMMYYITKAKKIKKENITIQTIPGRAETINKLSF
                     FVVNRDKMDEVLKPLIEESK"
     gene            complement(11621..11881)
                     /locus_tag="PKU88_08315"
     CDS             complement(11621..11881)
                     /locus_tag="PKU88_08315"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPX69317.1"
                     /translation="MNEYYVAVFDSTHYALKFEKAVRDWGFNISIMPVPREITASCGI
                     SAKLKADEIENIMALAVKNDLKVSGYYHVEVQNGRKVYHKST"
     gene            complement(11905..12414)
                     /locus_tag="PKU88_08320"
     CDS             complement(11905..12414)
                     /locus_tag="PKU88_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPX69318.1"
                     /translation="MNKNVELWDILDEDGRLTGRTIERGKPLKKGEYHLIVHIWIRNS
                     KGEYLIQKRSSQAGAWPGVWGAAGGSAISGESSLDAALREVKEELGLSFEPDALKRLY
                     RVRYKSVFSDLWFVCRDINPEDVRIQKEEVSEVKYACMDEILSMMESNEFINFGEAYM
                     SFLVNPCNC"
     gene            complement(12536..13318)
                     /gene="aroD"
                     /locus_tag="PKU88_08325"
     CDS             complement(12536..13318)
                     /gene="aroD"
                     /locus_tag="PKU88_08325"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPX69319.1"
                     /translation="MRLQAKKPIKIGNRTIGGPDMLICIPILEEEESGLERAAKNTIQ
                     LCPDIIEWRADYFKDACSPIKVMKALNMLRSIIGEIPLIFTFRGSFEGGFIEVEENIR
                     YEIIRLVLCTGEVDAADIELASRKNAVEEIRKEAYDHDIPLIISYHNFDETPSVEFLL
                     NKIREEVALGADIAKIAVMPKNEEDVLKLLSATLKARIELPYTPLITVSMGALGIISR
                     IAGGMFGSDMTFGAVEKTSAPGQIPIAELRASIKTLLYRIRR"
     gene            complement(13364..13813)
                     /locus_tag="PKU88_08330"
     CDS             complement(13364..13813)
                     /locus_tag="PKU88_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPX69320.1"
                     /translation="MNILVSACLLGTNCKYNGGNNFTPKLLELKRENNIIPVCPEELG
                     GLLTPREPCEIQEGSGKEVLEGKSRVINKIGEDVTADFIKGAGETLALAKQHNCRLAV
                     LKSRSPSCGCGIIYDGTFSGKLKEGNGVTAQLLLDNKIVVMTEKDFE"
     gene            complement(13827..16172)
                     /locus_tag="PKU88_08335"
     CDS             complement(13827..16172)
                     /locus_tag="PKU88_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HPX69321.1"
                     /translation="MKDKALRILEYNKIIDMLCENTVSPMGRDIAADLKPSGDLNEIK
                     ELLQETTEAVNLTLKKGSIPIGGVQDIRMPLKKVKMGSALEPGELLKVADTLRAARRL
                     KAFMNEDRGEESFPIIEYYIDTLSAFKGIEDRINSAIISEEEIADNASPQLSTIRRKI
                     KEKTASMREKLNHMISSPAYQKYLQEPIITVRGDRHVIPVKQEFRANIPGIVHDQSAS
                     GATLFIEPMAIVEMNNDVKKLKLDEENEIERILRELTALVDEKYDNIKTNMEILATLD
                     FIFAKARFSLDFRCTEPRVNNKGIVNIKKARHPLLKAESVVPIDIYLGETFNTLVITG
                     PNTGGKTVTLKTTGLLTLMAQSGLHIPANDYSEVAVFDEVFADIGDEQSIEQSLSTFS
                     SHMTNIVSILEHVTPNSLVLFDELGAGTDPTEGAALAMSILELLHKKNIRTAATTHYS
                     ELKIYALTTEGICNASVEFDVATLRPTYRLLIGIPGKSNAFEISKKLGLSEEIIKRAK
                     EFISQDDVKFEDLIKSLEEDKRKAEEERDVASRLKFELEKEKSEMDRRLEKLEAQKDR
                     IVREARSEALEIVRRSKEEAEELIRELRDAAMIEKSRMKLKQRENEIEDKIGESLFKK
                     TGSAPLKDIKIGMTVKIVNLDRKGSVLTLPDEDGNLTVQAGIMKINVNVNNLTAAEEE
                     KEKPKRSSGVKVNSAKAMAVASQIDLRGQTLDEALMNVDKYLDDAYLGNLPQVTIIHG
                     KGTGVLRAGIMQLLKSHSHVKSYRSGGFGEGGIGATIVEIK"
     gene            complement(16221..17702)
                     /locus_tag="PKU88_08340"
     CDS             complement(16221..17702)
                     /locus_tag="PKU88_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX69322.1"
                     /translation="MRSYGIAFKLWASIIAMTLALLVFTLIFQTEFLNDFYFELEKEQ
                     LEKDCFRLANYVARDQHFTIPYYSVMRRVNDIIIITGNDGKIIYVEGTNKYKLDSIFG
                     KNHIGRILKGEPVYEQSKEISNPHSVWPKEMDTLLVGVPVKKNVVLTQENRQRISENY
                     EGGPLKQHEITGAVYIITTLEYMQTTIDAIRKQFLYIFITAIVLASAISYFLSQSFSR
                     PLKLINNAALEISKGNYNTKIDLRSSREIKELGDTINNLAKQLTRVEQIRREFIANVS
                     HEIRTPLSYLQGYSEVLQDGLVESEEERNKYLGIIMDETARLKRMVDEILQLSQMEAG
                     YVQLQSMPFSMDGMIRSMIDKMLPHALKRNIGIKFTKTPEDALNCFGDENRIKQVLIN
                     LIYNAIKHSYDNGEILISAYRQGENIYVCVRDFGEGIPEVDLPFIWDRFYIVDKKRSE
                     NSTGLGLAIVKNIINAHGCEINVKSVNGEGSEFCFYLPAHKEM"
     gene            complement(17699..18385)
                     /locus_tag="PKU88_08345"
     CDS             complement(17699..18385)
                     /locus_tag="PKU88_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX69323.1"
                     /translation="MNNSVRVLLIEDEEKMRELIKIVFRKEDFVIIEAVDGKQGLNLF
                     RTNSVDIVILDIMLPEIDGWTVCREIRRTSNIPIILLTARGEEFDKLFGFELGADDYL
                     VKPFSPKELMARVKALLRRAEIKSNEAVSYYRFGDIVIDRLSREVTVNGRPVNLTNKE
                     YELLYFLAANPKIVFTREQLLLKVWGYEQYGDPRTVDTHIKKLREKLGDYSSCISTMW
                     GVGYKFEVPK"
     gene            complement(18401..20875)
                     /locus_tag="PKU88_08350"
     CDS             complement(18401..20875)
                     /locus_tag="PKU88_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HPX69324.1"
                     /translation="MNRTELLAPAGNFEALAAAVESGADAVYLGGNKFNARAYADNFD
                     GQSLTKAVNYAHIRGVRVFVTVNILLSDRELAEALDYVNFLYQIGVDGIIVQDIALLK
                     LARNAFPDLPVHCSTQMTVHNAEGARYYSELGAKRIVLARELTLEEVRGIAESTDAEL
                     EIFIHGALCVSYSGQCLMSSLIGGRSGNRGRCAQPCRKKYSLYDFNTGKAVIENRHKH
                     LLSTRDLNTYNRLKELTESGVASLKIEGRMKKPEYVAIVVRHYRNALDNIIKGEAFDT
                     ADAAYELKSAFNREFTEGYLFNRRNRDVVSIDRPDNRGVRLGKAIRQKGEIVSLLIEE
                     NLLHDGDGIEIISKSGGSTGAIISGIRVMGRNVKAAVKGDIAEVFIRDRVEAGSRVNK
                     TFDSELSRRAREEYSPENTRKIPVRCSLTVKTGEYPVITVNDDDGNTVSYTDEERAEA
                     AAKAATPIEKILEYLGKTGDTPYIIDVTETIIDGDCYIPARQINRMRRKVLELLSRIR
                     SQKYDRPIIQINAAELINGLISPVKASGEDSLEYIAGIRNYASAVSALNSGADSVYIL
                     SDVYEGDIVSETVKIAETCRSMGKTAFYVLPNIMTDRETGKIKERLEEIIKAADMRYF
                     GVVVSDAGQLKLARNSGIRRVRVNYSMNVFNSVSASHWHREGAEAVGLSAELNLPQIK
                     AITSNTAAAAESVVYGYLPVMTTEYCPVSMFRDNCNYPRGCNSSNYGIIDEKGKIFRI
                     KKLDSCRTQLLNSNVLLMAEDLGDVIDSGVMKLRADFYIEVPEEVGRIMKLYRNYSRM
                     NDDDRSLIKEIRGSGFTKGHFYRGVD"
     gene            complement(20914..21471)
                     /gene="zapA"
                     /locus_tag="PKU88_08355"
     CDS             complement(20914..21471)
                     /gene="zapA"
                     /locus_tag="PKU88_08355"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPX69325.1"
                     /translation="MAEKNKTTVSIFGTEYVMVAEKSEEYIYDLAAKVDEAMKEIARS
                     NSRYNPTMVAVLTALNLADELQKSQDELAETAEKLENIQGEMQRPFEELNEMRQELEA
                     IKEQYTKMQSEYTRSQIELGKISREWAKAQEELRDLRCELDVSRQTIEDVQNKLFENQ
                     IELLKTKKELDETKIKRIDKNRNVK"
     gene            complement(21624..24017)
                     /gene="pheT"
                     /locus_tag="PKU88_08360"
     CDS             complement(21624..24017)
                     /gene="pheT"
                     /locus_tag="PKU88_08360"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPX69326.1"
                     /translation="MLVPVKWLKDYVDIKFDPKGFADAMTMSGSKVEGIEELGKEIEN
                     VVVGKILKTEKHPDADKLTVAQCDVGTEVIQIVTGADNIKEGDYIPIAKHGSTLPGGV
                     KIKKGKLRGVESNGMMCSAQELALDLDKVSENMLDGIYILDGEYPLGADIKDILGLND
                     TVVEFEITNNRPDCLSILGVARETAATFGVAMKYPDTGFKENSENIKDYIDIEVKNSE
                     LCPRYMARMVKNIVIKDSPAWMQERLIKAGVRPINNIVDITNFVMLELGHPMHAFDYR
                     DLEDKKIIVRNAAPGEKITTLDETERKLDESILVIADGKKPTGIAGVMGGYGSEIKND
                     TTAIIFESANFNPVNIRMTSKKLGLRTEASSRYEKGIDPELAEMALNRACSLVSRLGA
                     GEIVGGSIDIYPSPVKVRKLRLNIKKANDFIGVQDITKEMAVKYLTTLEFKAEIANGD
                     LEVTVPTFRDDVEGEADLIEEIARLYGYDKIPVKLMDTTFTQGGKNNRQVIRDLAKSN
                     MAAQGLYEVVTYTFVSPAVYNKINLKAENPLRNAIKLLNPLGEDQSIMRTTIIPNMLE
                     VVSRNYNMKVSEGRFFELSKVYLAEELTVEKLAEERETLTIGMYGDVDFFDLKGVIEN
                     LMDELHIDKYRILSSNNDSMHPGRTAELLINNRRIGCLGEVHPEVLDKYDIPVRVYIT
                     ELNFEEILRQCDMNIKYKALPKYPSVARDIAIVAAEEITAGQIEEIIRNKGGKLVEDV
                     KLFDIYRGSQIKEGYKSMAYSIVYRSDEKTLSEEDITKVHNKIINSLENQVGAELRQ"
     gene            complement(24049..25068)
                     /gene="pheS"
                     /locus_tag="PKU88_08365"
     CDS             complement(24049..25068)
                     /gene="pheS"
                     /locus_tag="PKU88_08365"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPX69327.1"
                     /translation="MKETLEKIKLHSVKELESVQSQKELEEVRIKYLGKKGELTQVLR
                     GMGGLSPEERPVIGQLANEIRDYINEKIEETAYCLKRKDLEIRLGKEFIDVTMPSKRK
                     ALGNKHPLTKVQDEINQIFIGMGFTIVEGPEIEKSYYNFDALNHSKYHPARDMQDTFY
                     FTEEILLRTQTSPMQVRTMELQKPPIRVLVPGKVYRRDEIDATHSPMFHQNEGLVVDK
                     GITMANLKGTLDVFAKKLYGEDTKTRFRPHHFPFTEPSAEMDCTCSACKGAGCRVCKY
                     EGWIEILGAGMVHPNVLRNCGIDPEIYSGFAFGMGLDRIALLKYDVEDLRLFFENDKR
                     FLSQF"
     misc_binding    complement(25127..25353)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25509..25859
                     /locus_tag="PKU88_08370"
     CDS             25509..25859
                     /locus_tag="PKU88_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69328.1"
                     /translation="MLDRRKGPDKLVKWVKWSGIISWILVAIVLFITIIAKPSFESYM
                     DKSFHVKLQSNWDINLMQYVLVLLVLLFFFCIISIAINLSRCRRKTDRFNKTLILNAV
                     ASFIGILLYLLFFI"
     gene            complement(25842..27200)
                     /locus_tag="PKU88_08375"
     CDS             complement(25842..27200)
                     /locus_tag="PKU88_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPX69329.1"
                     /translation="MKKHQFSILLAYILILLISSGCAEAFQYSIGCADIKTNQSFSFF
                     IVSDPHYISKETYDNGKAFQDFLASGDGKMLNHTDELLDALIKDIRVENPDFLIITGD
                     LTCNGTRQSHIELAEKLKTIEDIGTCVFVVPGNHDIQNPYARKYIGDEVTDIETITSE
                     DYVNLYDPFGYEGCVSRDPNSLSYLAMPAEDIWLLMLDSTDTDRNMARKYPEQSGYLR
                     PDTLKWIEQCAALAKENNAKLIAVMHHSLIDHSEMIKANYTIKNSKDALKVFRKHGIE
                     IVFTGHIHIQDIKSDEYQGNRIYDIGTSSLAVYPHQYGRMKFTPYVGFDYRTIPLDME
                     YYDIHENAKDGTPLNFEDYSASFFTEQCGRMHQECLKQLEGLSDAERETVLETVCRMN
                     MLYFAGYRNEALNDIVNTEGFKKLENIAPCFTKEYAMKMLDDERTNNNTLLIPISLNE
                     KQ"
     gene            complement(27351..27557)
                     /locus_tag="PKU88_08380"
     CDS             complement(27351..27557)
                     /locus_tag="PKU88_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69330.1"
                     /translation="MDKIKLTEDKKKGSGWGTRLLMITGIAVVLLSAMALYLMRPSPV
                     EQDAGLIDEEESEEDKVFDKAESG"
     gene            complement(27702..27778)
                     /locus_tag="PKU88_08385"
     tRNA            complement(27702..27778)
                     /locus_tag="PKU88_08385"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27742..27744),aa:Val,seq:gac)
BASE COUNT         6800 a         7226 c         4682 g         9291 t
ORIGIN      
        1 tgtcctccga tgcattattc ttctactgca catcaggcct ctctttttct ttcttcttat
       61 cacgcttttc cgtagtgacc agttccggct caaccctgtt aaacaccaaa tcgataggcc
      121 tatccttcgt aggcagaatt gagagtataa cctcattctt ttcattcact attgacagtt
      181 cctctgagat ttccaggtct gccaccctaa gaacctgccc tactttgtat ttcgctgcat
      241 caaccgtaat tgccggagga atcttctcaa cctcaccctc tatttccagt tctgtcatct
      301 gcttgttaat gacccagccg cttctttcaa tcttctcccc gtttaccacc agtatcggta
      361 cctctgcatg gattatttcg tgtaatgtaa caggctgaaa atccacatgt atcaaatgct
      421 gctgcaggtg gtccctctgt atctccttaa taactgcagg tatttcggtt ccgtctattc
      481 tgagcttcac tacaacattc tcaccgtttt tagcaataat attattaagc tcactctcat
      541 caatcttgag cagccttccg gcagagccct ttacgtaaag tattgcagga atacagttct
      601 ctttctttaa tttttttgac tgcttgctgc cggagccggt cctgttatca gctttcaact
      661 ctaccttctt cataaaaaac ctccataaat gatcttcatc tatggagtat tcccaataat
      721 tgctttaatt aaacaatcga attaattatc tttactgccc ttctttcagt attatgattg
      781 ttgaatagag tttttgctgc aggtttcgga tttcgagtgc ttacgatatg cttcgaagaa
      841 tttttttaat cgcttaattc gcaacgcgca acaattcatc tgggcttaca gattatctct
      901 tgaatccttg tattcagaat atccttggca tgagcaggct taataattac tgcagtatct
      961 gcacccctgc cggttcctcc tacagctatt acaggttcac catagggtat cagtcctgca
     1021 tctaatgcca ttactgaaat ctctacgcac acttttaccc cttgtccaag catccttaag
     1081 gagttagcaa ttatctcaac aggatttata cctccgaact ttgcgctgag agatctctca
     1141 gctccggaaa gcacatgtgt tgttgtaagt acctttaccc ctaatgccga taattcctct
     1201 ctatcattcc tattcatctc atttacaccc ggctgcccat aaccatatgc atgagttaca
     1261 caagtaattt ccattccaca tccaataaaa tgtctggcag tagccccggt atttgaagca
     1321 acgacaatgt gagcaatatt attctccctt gcagccttta taactaattc cgcaaccttc
     1381 tccgtgtttt tctttccttc ctcggtaaaa tacatagtat cactcctcat ataatagtat
     1441 tcgcaaggaa tttttagctg tatttcagga taatagcctt gcaatcaatt gtcctgttta
     1501 atgcttctct gctattcaat aacaatgtct atggcttgaa ttgttggcag aaaccattta
     1561 ccgcaagact atcctaatat tttcataaaa tatacgaata cattcacttc cctgtatttt
     1621 tcgcttaaaa acttaaaatt actcatttcc aatacattaa taatcctttc atctatcggt
     1681 gccattatct cacagtattt cattccccga tttatagcct gctgctctgc aaaatccacc
     1741 agcagcttca tagaccccat atccccgtcg atgaaaccta tattgagtcc tccatccata
     1801 tgtgaatcct ctgtcagaac cattactgaa tttaccttcc catcctgaag caccccgtaa
     1861 acgcatttat ccttcacaag cttgtctgtc aaatcataag tcgctttttt aaaaacccac
     1921 ccgtagctta tatacccgtt ggacatatag tatgagtttc ctctggttat caagtcccat
     1981 gctgtagccg tactcattat actgatgacc gtctttgaaa cattaggctc ttttgtatcc
     2041 ttcccaagct ccttataccc gtatgtaaaa tatccatcct tcttgaaacc caggccctca
     2101 acaatccgta tgcactcttt acttgcagtt tcggtgcaaa gtcttatggt gtccacagct
     2161 tccgcctttg ccctgcttat aaaatactga gtaatagctt ttccgaaacc ttggcctgta
     2221 tatttttcat ccgctcttat gccctcaaac caagcttcgg aatcactatg ctttgtatat
     2281 ttcgcaaagc ctatgacatc tccatccttt tcgcctattg tgaattctcc ttccgcatca
     2341 ctgacccaat ggtcaaaaac atgttgtatg aaatcatgcc cttcccaaat tttcgatgaa
     2401 atctccagca ccttgctttt atcagcctgt gatacttttc tgaacctgaa cacattgact
     2461 ccctcctctt cagtaagata tgaacctgtc acccagatat ttcttaatca gtgacgggtc
     2521 gctttccaaa tgcagcttga tagattccct gtcggaatct tcaaatatta cttcaactgt
     2581 tgcatcacct ttaagaaact gctctgcaac cgttttttca tctacttctc tgtattgcac
     2641 cttctttcgc gctttttcag gcttaataaa ttccctggca aagaaacttc cagaaatcct
     2701 tattacccta atataattca tcctgcgcct cctatgcgaa aacatatgta tttatagatt
     2761 tccctacata agccttataa tttcattatt gagacaagtc tccagattaa gctctttaat
     2821 actgcctccg aaatccaccc ttattcttgt gccccctttt ttaccaatca cctcttttac
     2881 tactccctca tagtatatct tatgataaac tttttgccct gcatgaactt ggctgctgta
     2941 atcaatggat ttttgaaatt cctcaataaa gcgtgacggt ctcaccgcct tgccgtatct
     3001 gcgctttgga acggcaatat acaattccct tttagccctg gtcatagcca catagaatag
     3061 gcgcctttct tcttccggat cggttcttaa ggcagaatca tcctttatat acggtgtaag
     3121 cccttcaaca gtaccgcata tgaatatcgt ttcaaattca agtcccttag ccttatgcat
     3181 ggtcaggagc ttcacattat tatgcccgga atttgtgtat tccttttgtc ccagcttctc
     3241 aacaacatct tccgtatgct gcatataatt taatatgctt ttgaatccgc ctgctgagct
     3301 ttcaatttct tcaattatct ctgacaggcc ttttgtgctt attccctttg aagctgcata
     3361 ttccttaagg tattcatcat agcccattac atttctgata tatcttactg cgtttaatgg
     3421 attcatccct cccagtcttt tcaagtcatt cttaagcccg aataccccat ttatctgcag
     3481 cctgttcaga ctgccctggc cgattatgcc ctggataaag tctccgccgc agttttctgc
     3541 cttttccatc atatccctgc ttatatacct tttaggcttg ttcataattc tgataaaatc
     3601 ccttgtgtcc cccaatcctg ttccaagctt cagataactg atgatatcct tgtatatcca
     3661 atgattgtat atagatatca taccatcaaa agtaataaaa ggaatcctgc aatccaaaaa
     3721 agcatcaata accgacctgg attggagatt tgtcctgtat atcacggcaa aatccgaata
     3781 ctccctgcca tttttgatca tttgcccgat tctggaggct ataattcttg cctcctgctc
     3841 aaaatcttcc gcttcgaaaa caacaggaag gcttccttgt ccgttcaccg cttcaaggtc
     3901 tttggaatac ctgttattgt tggtatttat gacagataag gcagaggtca atatattgct
     3961 tgtacttctg taatttgttt taagatatat tttactgcag cccgaataaa gcctttcaaa
     4021 attcaggagt atatcaggag ctgcacccct gaatccataa atagactgat catcatcccc
     4081 tacaatgaaa atattattga ggggctcagc tataagcttt attgcttcaa actgcagtgc
     4141 atttatatcc tgaaattcat ccaccaatat gtatttgaac ctttccctta cttctttaag
     4201 aatcccggag ttttcccgca gcatatgaaa gcagtcaaat aatatatcat cataatcata
     4261 tttaccggac ttttgcttgt attcactgta ttccttcatt attcctttaa gctggctgct
     4321 gctgcatgaa cccggcttga aatcttccgc ttcggacata gtacttatta tatactccat
     4381 atcaccgatg atatccctta cctgctcctc atcctctgta aagcctgatt ccatccttct
     4441 gacaaaattc ctgataatat taaacttatg gttttcatca atcaggtctt ccagcttata
     4501 tcccttatac tgccttagaa gcttgtagaa tattgaatga aaggtgccga agctcacctt
     4561 ttcgtccgcc cggtttccca aaagcctgcc gtaccgctcc ttcatttcat tggctgccgc
     4621 tcttgaaaag gtaacgacaa gtatgttttc agggggcaca gacttacatt ttatcaggtt
     4681 gattaccctt gaagttatta cagttgtctt ccctgtaccc gggcctgcca gcaccatgca
     4741 gggcccctct ccgtgctttg cagctgtcag ctgttccttg ttcaagcctt gtaaataact
     4801 ttccatcagt ataccttaat cctttatatt atatgtacat tatatcattt cttttaagaa
     4861 tgccaaaata aagaaaagct gcacacaaat gtgcagctcc ttccgtctat agctcttttc
     4921 ctttattgta cgccttgata ttaatatcta ccagtttggg attgattgtt tcccttatga
     4981 tttcttccca gtcaatttca ttcattttga gagcttttac caaagcacct aaaagcacta
     5041 tattctgagt cttgatattt cccagttcct ccgctatttt ccccgcatca attacaactg
     5101 ttttaaatga accctgcagt gcttctatta gtccccgggg gtattcttcc ttgccgctgg
     5161 caattggcat tgaaggaatc tcatagttgt tgactacaac aactccgtcc ggtttgagat
     5221 actcaatcca tcttacagct tccatcttct caaaagatac gagtatgtca gcctggcctt
     5281 tgccaattat aggtgagtaa acctttttgc cgtatcttac ttgtgtagta acacttccgc
     5341 ccctctgagc catgccgtgc acctctgaca ttttcacgtc gtaaccggct tttataagcc
     5401 ctgcggtcaa tatcctgctg acaagtattg ttccctgccc gccgacgcca accaataata
     5461 tgttctttac atcactcata ttattcacca accttctcta tagcatcgaa tttgcaaacc
     5521 tgcatacaaa cttcgcagcc tacacacatt gactgatcaa tatatgaagt gccatctttg
     5581 aagaatacgg cagggcatcc taccttaagg cacattttgc acttcttgca tttatccctg
     5641 tcgaccctgc attttgtctt gaaaggcggt aaacccttaa ttagcacgca aggcctcttg
     5701 gttactattg cataaggttc ttcactgttt actgcttctg tgaccacctc atccatagcc
     5761 tttaaatcac atgggtctac tgttttaacc gttttatagc ctattgccct caatacttca
     5821 accggatcta tctgtaccgc atcttcaccc attaaagtct ttcctgttcc cggattctcc
     5881 tggtgcccgg tcattcccgt aattctgttg tcaagcacta caggcaccat tttaccttta
     5941 ttgtatacta tgtctattgc tcccgtaatt cctgaatgga agaaggttga atcacctaca
     6001 acaccaaaca ccttgttgtc ccgtccggat gcctcaaagg ccttagccat tcccattccg
     6061 gcactaaagc ctgctcccat acatatgcag gtatctactg cattaagagg tggtgaccct
     6121 ccaagagtat aacagccaat atcgcttgtt actactgtat tctttttctt tgacattgta
     6181 taaaagaaac ccctgtgagg acagccgggg cagagcaccg gaggccttcc tactactttg
     6241 gcatcaacct tctttgtctc gttttcctta ttcaaaaggg ctttcgctac tatatccgga
     6301 ttaagctcat acatcggagg tataagctct ttgcctatgc acttgactcc ttcggctttt
     6361 atctgttctt ccataaaggg gtccagttct tcaacaacat aaacagtctc aaatttagaa
     6421 gcaaattctt ttatcagctt cattggcagc gggaatgtaa aaccgagctt taaatacgaa
     6481 gcattgtctc cgaatacttc cttaacatac tgataagata ttcccgcagt tattatacct
     6541 atttttgtgt ccttgttata ttcaatcttg ttgagaggtg agctgtatgc atattcttca
     6601 agagatttca ttctttcttc aacttttata cgggcaatct tcgcattagc gggagttgct
     6661 acgtacttgg ccgcatttct ctcataactc ttcggctcaa cttctattct ctctccaaac
     6721 tctaccatac tcttgctgtg gcataccctg gttgttaccc tgtacagtac aggagtatca
     6781 aactgctcgc tgattttaaa tgcctccgcc ataaagtcct ttgattcctg gctgtcgctg
     6841 ggctcaacca tcggaatctt ggcaaatttt gcataatacc tgttgtcctg ctcattctgt
     6901 gagctgtgca tgcttgggtc atcagcggta ataagtacat accctgcatt cactcccagg
     6961 tatgcatatg tgaatatcgg gtcagctgct acgttgaggc cgacatgctt cattgcggat
     7021 atgcttctca ctcccgcaat agatgcacca atcgcaactt ctgttgccac cttctcgttg
     7081 ggtgcccatt cacaataaat atcatccttg tattctacga tgttttccag tatttctgtg
     7141 cttggcgtac cgggataggc tgcagctact ttaaccccgg cttcatacgc gcctctggca
     7201 attgcttcgt tgcctgacat cagttgcttc actatgttta cccccttata tattaattat
     7261 tagctactac acttccaagg gctattgata aaagctttga ttctgaggag tcagctcttg
     7321 atacaagtac tataggcact ttggcaccca ttataactcc cgcggatttt gcccctgcaa
     7381 aatacgtcat tgcctttccc attgcatttc ctacttcata ataaggaacc agcaatatat
     7441 cggctttgcc gccgccttct cccttaaagc ctttatgggc acatgcctca ctgcttattg
     7501 caaggtctaa agcaacaggc ccctgcacta caactccttg ttcttcccat ttgtctttca
     7561 tatcctcaat tgcttttgca tcaacagtcg ccggtatttt gggattaact gtctccgcac
     7621 ccgcaataca tgaagcataa atttttttca ttttcattgc cttgcacact ttaactgcat
     7681 tttccagtat ctgaaccttc tgagaaagat cgggtgccac attcattcct ccgtcagtta
     7741 aatacaaaag cttattatat gaaggaacct cgtataccat aacatggctg ataagattat
     7801 ccgtcctcag tccttcctcc ttgtcaagaa cggctctcat tatatcggca gtctgaagaa
     7861 ttcccttcat caggaagtca cctttgcctt ctcttacgag ctttacagac ttccttgcag
     7921 cttgtatcaa atccggttca tcaattatct caaattcact gctttcaatt cccaactcat
     7981 tcatgatttc gagtattttt gctttatttc ctaccaaaac cggctctgca atacctattt
     8041 cttttgcctg gcttactgcc ttgattacgt cttcatcctg agctgcggct ataacaattt
     8101 tcattatttt ttggcttttt gccgtttcca gtaattcatt aaaattctta atcattattt
     8161 cacctcaaaa tattttgtat atcttaaaaa ttttgtagaa ccccatattt attctacaat
     8221 ataatttatc ttccttcaac ctataactaa tatttttaag tattatttca aaaataaaga
     8281 cacgaaggac gtttcgggac acggggacgt ttctcctgtc ccgctgaaag cgggacagga
     8341 gaaacgtccc cgtgtcccga aacgtcctcg tgtccttcgt gtccttcaag ctgatactta
     8401 ttaatacccc agttcttttc ctataataat tacctcaaat tcggtcttgt acggccttct
     8461 ttcaataatt gtggtaacgc tgcacattct ctgcgggctg ttgcagtcat tgcatttctc
     8521 ggtatgcgca catggagtat caagctttag ccttcttgca tttttatatg tattctcttt
     8581 aatccttttt aaagctgcat ccaggtcttt tgttatctta ttgactccgc acaaaacaaa
     8641 tacttttttg ggtccaaaaa tcattgaagc aactctgttc cccaagccgt caatattaac
     8701 cagttttccg tcctcggtca gggcatttgt acccgacaga tatacatcgg ctttttgtgc
     8761 tttgtccctt gcataatcca ttccttcagg agtgctctcc agccaatgga aatataccct
     8821 gttgcccctt tcaataagct tctggggtat acccagggag tatacggtca tggatcctcc
     8881 gattcctact gttgctccat ccccgatttt acccatcaaa taggatactg ccgtatcgga
     8941 gtcatcaaaa tatgatgcct tcatattgtg cttttccaaa gcttctattg tttttttaac
     9001 agattcattc atactatcac ccccaagata tattatattt caagcaatgc ctggaattca
     9061 agcactacag gtcgtaatac agctcaaatt ctgccggatg aggaattatg cttatcttct
     9121 tatgctcctt ctcaatcttg ttctttatcc aagtttcaat taggctcttt gtgaacaccc
     9181 cgcctttcag caggaattca tgatcctgcc ttagagcttc catagcctcc tcaaggctct
     9241 ttggcaggcc ttttatcttc ttcttctcct catcatcaag ctcaaaaata tttacatcgt
     9301 atggtccgaa gccctccttg acagggtcaa ttttgtttat tataccatcc agaccggcca
     9361 tcagcagtgc tgaataagca aggtaaggat tgcaggtggc atcggaagat ctgaactcaa
     9421 acctcttgct gtcaggagta ttgccataac ccggtattct aacgatagcg cttctgtttg
     9481 cagttgcaaa acatatgctt accggtgcct cataacctgg aaccaacctc ttatatgagt
     9541 ttgtgctcgg gctcgtaaaa cctaacaatg cgggagaatg cttcaaaagc cctccgataa
     9601 aatacagtgc tgtctggctg aggccggaat atccgtcctt gtcatagaat aaaggctttc
     9661 catccttata cagccacata tgcacatgca ttccgcttcc cgcctcgcca aaaataggct
     9721 taggcatgaa ggtggcgctc ttgccgtttt gtattgctat gtttttcacc acatatttga
     9781 gcaaatatgt cttatccgcc atatccttca gtttccccgg ctctacttcc agttcgacct
     9841 gcgccggtcc tcccacctca tgatgatgat actttatggg catgccgaac tcttcaagca
     9901 taactgtcat tttgcttctc aagtcatttg tcagatcata gggaagcgcc agatgatatc
     9961 cgcccttatg ccttactttg tagccaaggt ttttttcata ctggtcatta gtattccatt
    10021 cagcctgcgc cgaatctatt gccacctcct ggtgctccgg cctgctttcg taagctatct
    10081 ggtcgaataa ataaaattca aactccggtc cgattatatt ctcatccgct ataccggtag
    10141 aggccatgaa cttctcagcc ttttctgcga tatatctggg atccccttca tatcgcctta
    10201 tcccgccctc aagtgcaaag atatctccta tcatgctgag agtcggaatt tcacaaaatg
    10261 ggtcaacaaa agccgtattc aagtccggta taaaaaccat gtcacttttt tctacagtct
    10321 tgaaaccata gttggaaccg tcaaatccta taccctcctc aagtactttc tcattcagcc
    10381 tggtcaccgg aatagagagg tgatgccatc tccctgctaa atcagtaatt ttgaagtcta
    10441 ctattttaat gccctttgaa ttacaaaatt ccttgacctc ggtgagattt ttaaacaata
    10501 caataacccc cttgtaatat tgtgctaatt ttagtatatt taaacatacg gaataagtca
    10561 aacaattaaa aaacgcggca taagccgcag tcttctattt actttcttct attaaaggct
    10621 ttagtacctc atccatcttg tccctgttta ccacaaagaa agacagtttg tttatggttt
    10681 ctgccctgcc gggtattgtc tgtatggtga tattctcctt ttttatcttc tttgctttgg
    10741 taatatagta catcatctgt tttggcggca tatttgtctt tacatattcc ttgacaggct
    10801 cctgaagctt aagaaagtta gcagcaatat tgcctctcat tactttatca atacctaatc
    10861 tgactatctc ctgctgcatc ttcaccctgc ccaggtctcc ctccttatag ccgccgccct
    10921 tgttgttctt acggtatctc aagagcttta atatatcctc ccccttgctc actacctctc
    10981 cttttttaaa atgtatatgc aaatcatcaa ccggatcatc atagttcata tccataggta
    11041 cggttacctt aatgccgccc atggcatcaa ccagttcttt gacagcctca taatccacct
    11101 ctgcaaaata atgcacatcc agtcccgtaa gagatacgac gctttcggcc acattatccg
    11161 cccctcccct tccgtaggaa gagttaatct tgttatttgc agagatctta tgtccttttc
    11221 tttcaatata tgtgtccctg ggtattgaaa gcacatccag cgctccatcc tctgggtcga
    11281 agcttgccag cattattgta tccgcaagac cctttgccac acccataact acaacattta
    11341 cccttttttc cggtaccgga tagccttccg caccctcctc cacgcttatt ttgcttaaaa
    11401 agttatatgt aaagccatat atggctgctg caatcagaag taccgcaacc acaacagctg
    11461 ttattatctt ccctttagca ctcaattttt ttcctccaaa tcgtattaga tattaatcca
    11521 tgtcacaagt aaataataat gcagacacaa cttatagttt aattctcctt atataatatg
    11581 taaagtgcat ttacagccaa aaagtatacc ataaaaacaa ttaagtgctt ttatggtata
    11641 ctttcctgcc gttttgtact tctacatgat aatatcccga taccttcaga tcatttttga
    11701 cggcaagcgc catgatattt tctatttcat cagctttcag ttttgctgaa attccgcagc
    11761 ttgcggttat ttcccgggga accggcatga tgcttatgtt aaaaccccag tcccttactg
    11821 ccttttcaaa cttcaaagca tagtgggtag aatcaaaaac ggcaacgtag tattcattca
    11881 ttgcattcac tcctcattat gtgcttaaca gttacaagga ttcaccaaaa agctcatata
    11941 tgcttccccg aagttaatga actcattgct ttccatcatt gacaatattt catccataca
    12001 ggcatatttc acttcactga cttcttcctt ctgtatcctg acatcttccg gattgatatc
    12061 ccggcaaaca aaccataaat ctgaaaaaac acttttatac ctgaccctgt ataatctctt
    12121 cagagcgtcc ggttcaaagc ttagccccag ctcttcctta acttctctca atgcggcatc
    12181 cagactgctt tcgccgctga ttgccgaacc tccggctgcg ccccagactc cgggccaagc
    12241 accggcctgt gaggaccttt tttgtataag gtactcacct ttgctgtttc ttatccaaat
    12301 atgaactatt aaatgatatt ctcccttttt taaaggcttg cctctttcaa tagttcttcc
    12361 ggtcaggcgg ccgtcttcat ccaatatgtc ccataactca acattcttat tcataaaact
    12421 gcctcatcct atacatttta ggataaacat cagctaatta tatttcggat atcctaaatg
    12481 ttttccttga ttcatagaat acgccaaagg ttctgtgcca tttttaataa gctgtttacc
    12541 ggcgtattct atataataag gtttttatcg aagccctcaa ttcggctatg ggtatctgcc
    12601 ccggagcaga agtcttctca acggctccga aagtcatatc tgacccgaac atacctccag
    12661 ctattctgct gataatgcca agagcaccca ttgatactgt tatgaggggt gtgtaaggca
    12721 actcgatacg ggctttcaat gtagccgaaa gcagttttaa cacatcttcc tcattcttgg
    12781 gcattaccgc aatttttgct atgtccgctc ccaaggcaac ttcttccctt atcttattca
    12841 ataaaaactc aacagaaggc gtttcatcaa aattatggta agatatgata agcggtatat
    12901 catggtcata tgcttctttt cttatttctt caactgcatt tttgcggctt gcaagttcta
    12961 tatctgcggc atcaacctct cctgtacata ataccagcct gatgatttca taccgtatat
    13021 tttcttcaac ctcaatgaac ccgccttcaa agctgcccct gaatgtaaaa ataagaggta
    13081 tttccccgat tatgcttctg agcatattaa gcgctttcat tacttttata gggctgcagg
    13141 catccttaaa ataatccgcc cgccattcta ttatatcagg gcatagctgg atcgtatttt
    13201 ttgctgcccg ctccaatccg ctctcctctt cttccaatat tggtatacaa atcagcatat
    13261 caggcccgcc aatggtccta ttcccaattt ttatcggttt ttttgcctgt aatctcataa
    13321 caaatctcct gttccaagcc caaaaacatt tcaggcttca tacttattca aaatcttttt
    13381 ctgtcattac cacgatttta ttatccagca gcagctgtgc ggtcacaccg tttccctctt
    13441 taagctttcc tgaaaaagtc ccgtcatata ttattccgca gccgcaggag gggcttcttg
    13501 acttaagcac cgccagccgg cagttatgct gcttggctaa agcaagggtt tctccggcac
    13561 ccttaataaa atctgccgtt acatcttctc ctattttgtt tatgaccctt gattttcctt
    13621 ccagtacttc ctttccggaa ccctcttgta tctcgcaggg ctcccttggt gtcagcaagc
    13681 ctcccagttc ttcgggacat acaggtatga tattgttctc ccttttaagc tccagcagct
    13741 tgggagtaaa attgttcccc ccgttgtatt tgcagtttgt acccaataaa catgcactaa
    13801 ccagaatatt catccaatca cttccatcat tttatttcca ctatggtagc cccaatgccg
    13861 ccttcaccaa acccgccgct tctgtatgac ttcacatggc tgtggctttt cagaagctgc
    13921 attatgcccg cccttagaac ccccgtgccc ttgccgtgaa ttattgtgac ctggggcagg
    13981 ttgcccaggt atgcgtcatc caggtattta tccacattca tcagggcttc atccaatgtc
    14041 tggcccctta aatctatctg tgaagcaaca gccattgcct tggcggaatt caccttgaca
    14101 ccggaactcc tcttgggttt ttccttctct tcttccgctg ctgtcagatt gtttacattt
    14161 acatttattt tcattatacc tgcctgcacg gtcagattcc cgtcctcatc aggaagtgta
    14221 agaacacttc ccttccggtc aaggttgact atcttcacgg tcatgcctat ctttatgtcc
    14281 tttaacggtg cgctgccggt ttttttgaac aatgattctc caatcttatc ttctatttca
    14341 ttttcccgtt gttttagctt catcctcgat ttttcaatca ttgcagcatc cctaagttcc
    14401 cggataagct cctccgcttc ctcctttgac cttcgcacta tttcaagggc ctcactgcga
    14461 gcctccctta caattctgtc cttttgagct tcaagctttt caagccttct gtccatttcc
    14521 gacttttctt tttccagctc aaatttcagt ctggaagcaa catcccgttc ttcttcagcc
    14581 ttccttttat cctcttcaag gctttttatc aaatcttcaa attttacatc atcctgagaa
    14641 atgaattcct ttgccctttt tattatttcc tcggaaagcc caagcttttt ggatatttca
    14701 aaggcatttg actttcccgg tatgccgatt aagagcctgt acgtcggcct caaagtagcc
    14761 acgtcgaatt ccacgctggc attgcatatt ccttcggtag taagggcata tatcttcagt
    14821 tcactataat gtgtagtggc agcagtcctt atattctttt tatgcagcag ttccagtatg
    14881 gacattgcca gtgccgcacc ctccgttgga tccgtacccg cacccagttc gtcaaacagc
    14941 accagggaat ttggggtgac atgctcaaga atggacacta tattggtcat atgggaagaa
    15001 aaagtactga ggctctgttc tatgctctgc tcatccccga tatccgcaaa cacctcatca
    15061 aaaactgcca cttcactgta gtcatttgcc ggaatgtgaa gacctgactg agccataagc
    15121 gtcagaagcc ctgtagtctt taatgtgaca gtcttccccc ctgtattcgg ccctgtaata
    15181 accagggtat tgaaggtttc tcccagataa atgtctatgg ggactacgga ctcagccttt
    15241 aacaaaggat gccttgcttt cttgatgttc actatgccct tattgttgac ccttggctcg
    15301 gtgcacctga aatccagaga aaacctggct tttgcaaata tgaaatccaa tgtcgccaat
    15361 atctccatat tggtcttgat gttgtcatac ttctcatcaa caagtgccgt aagctctctc
    15421 agtatccttt ctatttcatt ctcctcatcc agcttcagct tttttacatc attattcatc
    15481 tctacaatcg ccataggttc aataaaaaga gttgcaccgc tggctgactg gtcatggact
    15541 atgccgggta tatttgctct gaattcctgt tttaccggta tgacatggcg gtctcctctt
    15601 acagttatga tcggttcctg aagatacttc tggtaagccg gagatgaaat catatggttc
    15661 agcttttctc tcattgaagc agttttttct tttatttttc tccttatggt gctaagctga
    15721 gggctggcgt tgtccgctat ttcctcttcg ctgattatgg cgctgtttat tctgtcttct
    15781 atacctttga atgctgacag ggtatctata taatactcaa ttatcggaaa gctttcctca
    15841 cctctgtcct cattcatgaa agctttcaac cttctggcag ctcttaatgt gtctgccacc
    15901 ttaagaagct ctcccggttc cagagccgac cccatcttga cctttttcag gggcattcgg
    15961 atgtcctgta ctccgccaat ggggatgctg cctttcttta gtgtaagatt tacagcttct
    16021 gtggtttcct gaagcagctc ctttatctca ttgaggtcac cggagggctt caaatcagcc
    16081 gcaatatccc ttcccatagg tgaaacggta ttttcgcaca acatatctat aatcttatta
    16141 tattcaagaa ttctcagcgc cttatccttc aatccattca cctcttcacg caatccgcat
    16201 cccgcagccc gtaccttatt ttacatctcc ttatgggctg ggaggtagaa acagaattcc
    16261 gagccttctc cgttcacgct ttttacattt atctcacagc cgtgagcatt tattatgttc
    16321 tttactattg ccagcccaag gccggtgctg ttctccgatc ttttcttgtc aactatataa
    16381 aatctgtccc aaatgaaggg cagatccacc tcaggtatcc cttccccaaa atcccttacg
    16441 catacatata tgttttcacc ttgcctgtat gcgcttatca atatttcacc attatcatac
    16501 gaatgcttga tcgcgttgta aatcaaatta atcagcacct gttttattct gttttcatcc
    16561 ccgaagcagt taagtgcatc ctccggcgtt ttcgtgaatt taatgcctat attccttttt
    16621 aatgcatggg gcagcatctt gtctatcatg ctcctgatca ttccgtccat gctgaagggc
    16681 atagattgaa gctgtacgta tcccgcttcc atctgcgaca attggagtat ttcatccacc
    16741 atccgcttta accttgctgt ttcatccatt attataccaa gatatttatt tctttcctcc
    16801 tctgattcaa caagcccgtc ctgaaggacc tccgaatacc cctgcaggta gcttaacggt
    16861 gtccttattt catgggatac gttggctatg aactctcttc ttatctgttc aacacgggta
    16921 agctgctttg ccaggttgtt tatagtatcc ccaagttcct ttatctccct gctggaacgc
    16981 aggtcaattt ttgtattgta attgcccttt gatatttcca gtgcggcatt gttgataagc
    17041 ttcagcggcc ttgaaaagct ttgtgaaaga aaatatgata ttgcggaagc aaggactatt
    17101 gccgtgataa atatatataa aaactgcttc cttatggcat caattgttgt ctgcatatac
    17161 tccagggtgg tgattatata tacggctcct gtaatttcgt gctgctttaa tggtccgccc
    17221 tcgtagtttt cactgatacg ctgcctgttc tcctgggtca aaaccacatt ttttttaact
    17281 ggaacgccaa ccagaagtgt atccatttcc tttggccata ccgaatgtgg gttgctgatt
    17341 tctttgctct gctcgtagac tggttcccct ttaagtatcc tgccaatatg attcttccca
    17401 aaaatactat ccagtttgta tttgtttgta ccttccacat agattatttt tccgtcattg
    17461 ccggtgatta ttattatatc atttactctt ctcattacac tgtagtaagg tatggtgaaa
    17521 tgctgatccc tggctacata atttgccagc ctgaaacaat ccttctccaa ctgttctttc
    17581 tctaattcga aataaaaatc atttaagaat tccgtctgga atatcagggt aaaaaccagg
    17641 agcgccaatg tcattgcaat tattgatgcc catagcttga atgctattcc gtagcttctc
    17701 atttcggcac ctcaaactta tacccgacac cccacatggt actgatacag ctgctgtaat
    17761 ctcccagctt ctctctcagc ttctttatat gggtatccac agttcttggg tcgccgtact
    17821 gctcatagcc ccagacctta agaagcagct gctcccttgt aaagacgatt ttgggattgg
    17881 cagccagaaa atacaataat tcatattctt tattggtaag gttgacaggc cttccgttta
    17941 ccgttacttc cctggacagc ctgtcaatca caatatcccc aaacctataa tatgaaaccg
    18001 cttcattact cttaatttct gcccttcgca gcaaagcttt aactcttgcc atcagctctt
    18061 tcgggctgaa tggctttacc aaatagtcat cggcacccag ctcgaagccg aagagcttgt
    18121 caaattcctc cccccgtgct gtcaatagta ttatgggaat attcgatgtc cttcttattt
    18181 ccctgcatac agtccagcca tcgatttcag gaagcatgat atcaagtata acaatgtcca
    18241 cactgttcgt tctgaaaaga ttcaatccct gtttcccgtc aacggcctca ataatcacga
    18301 agtcctcttt tctgaaaact atcttgatta attccctcat cttttcttca tcttcaataa
    18361 gcagtaccct aacactgtta ttcacccgca gttcctcctt tcaatcgaca cccctgtaaa
    18421 aatgtccttt cgtgaatccg gatcccctga tttcttttat tagtgacctg tcatcatcat
    18481 tcatcctgct atagttcctg tacagtttca taattctgcc aacctcttcc ggcacttcta
    18541 tataaaaatc ggccctcagc ttcattactc cgctgtctat tacatctccc aaatcttcag
    18601 ccataagcag tacattagag ttcagaagct gcgtcctgca tgaatcaagc ttttttatcc
    18661 taaaaatttt tcctttctcg tctattatac cataattgga gctattgcat ccccgaggat
    18721 aattgcagtt gtctctgaac atcgaaaccg ggcagtattc ggtggtcata accggcaaat
    18781 atccatacac aacagactcc gcggcagctg cggtatttga ggttattgct ttaatctgcg
    18841 ggagattcaa ttccgcggat aatccgacgg cttccgctcc ttccctgtgc caatggcttg
    18901 cggatacgga attaaacaca ttcatgctgt aattgactct taccctgcgg atacctgaat
    18961 ttcttgccag tttcaactgt ccggcatccg ataccactac gccgaaatat ctcatatccg
    19021 ctgcctttat tatttcctca agccgctcct ttatttttcc tgtttccctg tccgtcataa
    19081 tgttcggaag cacataaaac gcggtcttgc ccatacttct gcatgtttct gcgattttta
    19141 cggtttcgga aacaatatcc ccctcatata catcactgag aatatatacc gaatcagctc
    19201 ccgaatttaa agcacttaca gcagaggcat agtttcttat ccctgctata tattccaatg
    19261 aatcttctcc ggaggccttt accggtgata tcaaaccgtt gataagctct gcggcattaa
    19321 tttgaataat cgggcggtca tatttctgtg agcgtatccg tgacagcagt tccagcactt
    19381 ttcttctcat ccggtttatc tgccttgcag gtatgtagca atccccgtct attattgtct
    19441 cagttacatc tataatgtaa ggggtatccc ctgtttttcc aagatactcc aatatttttt
    19501 caatcggagt ggcggccttt gcagcagcct cggccctttc ctcatcggta tagcttacag
    19561 tgttgccgtc atcgtcattt actgttatta ccgggtattc tccggtttta actgttagac
    19621 tgcaccggac agggatcttc cttgtattct caggtgaata ctcctctctc gcccttctgc
    19681 tcaactcgct gtcaaaggtt ttgtttaccc gggaaccggc ttcaaccctg tctcttatga
    19741 aaacctccgc aatatctcct tttactgcgg cttttacatt cctgcccatg acccttatgc
    19801 cgctgattat tgcaccggtg cttccgccgc tttttgatat tatttcgatt ccgtccccgt
    19861 catgcaataa attctcttca attaaaaggg atactatttc acctttctgc cttattgcct
    19921 ttcccagcct tacacccctg ttgtcgggcc tgtctatgct gaccacatct ctgtttcttc
    19981 tgttgaacag gtatccttcg gtaaactccc tgttgaatgc acttttcagt tcataagcgg
    20041 catcggcggt atcgaatgct tcgcctttta ttatattatc cagggcattc ctgtaatgtc
    20101 tgaccactat agccacatac tcgggttttt tcattctccc ttctattttc agggaagcca
    20161 ctcctgactc agtcagctcc ttcagcctgt tgtatgtatt aagatccctg gtactcagca
    20221 aatgcttgtg ccggttctca atcaccgctt ttccggtatt gaaatcatac aaggaatatt
    20281 tctttctgca gggctgggcg catctccccc tgtttccgct ccttccgcct ataaggctgc
    20341 tcataaggca ctgccccgaa tagctcacac acaaagctcc gtgtatgaat atctccagct
    20401 ccgcatctgt gctttcggca atgcccctga cctcttcaag ggtcaattcc cttgccagca
    20461 ctatcctttt ggcgcccaat tccgaatagt acctggctcc ttccgcattg tgcaccgtca
    20521 tctgagtgct gcagtgaacc ggcagatccg gaaaggcatt ccgcgccagc tttaggagag
    20581 caatatcctg gacaattata ccgtcaacac ctatttgata taagaagttt acatagtcca
    20641 gagcttccgc taattccctg tcggacagca gaatattaac ggtgacgaat accctgacgc
    20701 cccttatatg ggcgtaatta accgcctttg taaggctttg gccgtcaaag ttgtcggcat
    20761 acgccctggc attgaattta tttccgccca aataaacagc atcagcacca ctttcaacag
    20821 cagcagctaa tgcttcaaaa ttcccggcag gagccagaag ttctgttctg ttcattatca
    20881 tcacttcccg tgtctcgtgt cctgtaatta tttttacttt acattcctgt ttttatctat
    20941 tctttttatt ttcgtttcat ccagttcttt ttttgtcttc agcagctcta tctggttttc
    21001 aaaaagcttg ttttgaacat cttcaattgt ttgcctggaa acgtccagtt cgcaccttaa
    21061 atccctaagc tcctcctgtg cttttgccca ctccctgctt atctttccca gttcaatctg
    21121 tgaccttgta tactcactct gcatttttgt atactgctct ttaatggcct ccaactcctg
    21181 gcgcatttca ttcagttcct caaagggcct ttgcatttct ccctgtatgt tctccagctt
    21241 ctcagcggtc tcggcaagct catcctggga tttttgcagt tcgtctgcca gattcagggc
    21301 agtaagcaca gcaaccatag tgggattata ccttgaattg ctccttgcta tttccttcat
    21361 cgcttcgtca accttagctg caagatcata tatatattct tctgactttt cagcaaccat
    21421 cacatattca gtaccgaata tgcttaccgt tgtcttattt ttctcagcca tatatatcaa
    21481 ctcctttggg attaaaaact atatctaaat attattcttc aaaatgcgcg gattttcctt
    21541 catttttttt acattaaggc aaaataattg tgaaacagca tatcaactca cactgtatac
    21601 cgctgatacg ctgtcccatt attctactgt cttaattccg ctcctacctg attttcaagg
    21661 gagttaatta ttttgttgtg aacttttgtt atatcctctt ccgaaagagt cttttcatcg
    21721 gatctgtata ctatggagta agccatactc ttatagcctt cctttatctg gcttcccctg
    21781 tatatgtcaa ataattttac gtcttcaaca agcttgccgc ctttattcct gattatctcc
    21841 tctatttgtc ccgccgtaat ctcttcagcc gcaacgattg ctatatccct tgcaactgac
    21901 gggtacttcg gaagtgcttt atactttata ttcatatcac actgcctcaa tatctcttca
    21961 aaattaagct cagtaatata aacccttacc ggtatatcat acttgtcaag cacttcagga
    22021 tgcacttctc ccaggcagcc tatcctccgg ttatttatca gaagctctgc agtccttccc
    22081 ggatgcatgc tgtcattatt tgaggacaat atcctgtatt tgtctatatg caattcatcc
    22141 atcagatttt caatgacacc cttcaggtca aagaaatcca catctccgta catgcctatg
    22201 gtaagagtct ctctttcctc cgcaagcttt tccacggtca gctcctccgc caggtacacc
    22261 ttggaaagct caaagaaccg cccctcactt actttcatat tgtagttcct tgatactacc
    22321 tccagcatgt tgggtattat tgtagtcctc attatactct ggtcctctcc aagaggattc
    22381 aacagcttta ttgcatttct aaggggattt tctgctttga ggtttatttt gttgtatacc
    22441 gccgggctga caaaggtata agtcactacc tcataaagcc cctgggcagc catgttggac
    22501 tttgcaagat cccttattac ctgcctgttg ttctttccgc cctgtgtaaa tgtagtgtcc
    22561 atcagtttta caggaatttt atcatagccg taaagccttg caatttcttc tatcaaatcg
    22621 gcttcaccct caacatcgtc tctgaaggtt ggtaccgtta cctccagatc cccgttggct
    22681 atctctgcct tgaattccag agttgttaaa tacttgaccg ccatttcctt cgttatatcc
    22741 tgaaccccga tgaaatcatt cgccttcttt atattcagcc tcaattttct gaccttcaca
    22801 ggagaaggat agatatcgat gcttccgcct acaatctcac cggcacctaa ccggctaaca
    22861 aggctgcatg ccctgttcag cgccatttcg gcaagctccg ggtcaattcc cttttcatat
    22921 cttgaggaag cttcagtcct aagccccagc tttttggaag tcattcttat attcacaggg
    22981 ttaaagttcg cactttcaaa tataattgct gttgtgtcat tttttatttc actgccgtaa
    23041 cctcccataa ctcccgcaat accggtaggt ttttttccgt cggctataac aagtattgac
    23101 tcatccagct tccgctccgt ttcgtcaagg gttgttattt tctctccggg cgctgcattt
    23161 cttactatga tttttttgtc ctccaaatcc ctatagtcaa aggcatgcat gggatggccc
    23221 agttccagca taacaaaatt ggttatatca actatgttgt ttataggcct gacgcctgct
    23281 tttataagcc tttcctgcat ccatgcagga gaatccttaa tcactatatt cttaaccatt
    23341 ctggccatgt atcttgggca aagctcgctg ttttttactt caatatcaat ataatccttt
    23401 atattttcac tattttcctt aaaaccggta tccggatatt tcatagcaac tccaaaggtt
    23461 gctgcagttt ccctggctac acccaaaata cttaaacagt caggcctgtt gttcgttatt
    23521 tcaaattcaa ctacagtatc attaagtccg agaatatcct ttatatccgc accaagaggg
    23581 tattccccat ccaatatata tattccgtcc agcatatttt cagacacttt gtcaagatcc
    23641 aaggccagct cctgagcaga gcacatcatt ccgttcgatt ctactcccct gagctttccc
    23701 ttcttgattt ttaccccgcc gggaagtgtt gaaccatgtt tggcaatggg tatatagtcc
    23761 ccttccttta tattatccgc ccctgtgaca atctgtatca cctcggttcc aacatcacat
    23821 tgcgcaacag tcagcttgtc ggcgtccgga tgcttttctg tctttaaaat ctttcccacg
    23881 acgacatttt caatttcctt tcccagttcc tctattcctt caacctttga ccccgacatg
    23941 gtcattgcat cggcaaagcc cttcgggtca aacttgatat ccacataatc ctttaaccat
    24001 ttaacaggta caagcatgtt taatctcctt tctttcctta ttacttactt aaaactgcga
    24061 caggaacctc ttgtcattct caaagaaaag cctgaggtcc tccacatcgt atttcagcag
    24121 cgcaatcctg tcaaggccca tgccgaaggc aaagcctgaa tatatctccg ggtcaatgcc
    24181 gcagttcctg agcacattgg ggtgtaccat accggcgccc agtatctcaa tccatccttc
    24241 atatttgcat accctgcagc ctgcaccctt gcatgcggag caggtgcaat ccatttccgc
    24301 gcttggctca gtgaagggga agtgatgggg tctgaacctt gttttggtgt cctctccgta
    24361 cagcttcttt gcaaagacat ccagggttcc cttcaaattc gccatagtga ttcccttatc
    24421 tacaaccaaa ccctcattct gatggaacat aggcgaatgt gtcgcatcta tttcatctct
    24481 cctgtatacc ttccccggga ccagcaccct tatcggaggc ttctgcagtt ccatagtcct
    24541 gacctgcata ggagaggtct gggtccttaa caatatttct tcggtaaaat agaaggtgtc
    24601 ctgcatatcc cttgcaggat gatacttgct gtgatttaac gcatcaaagt tataatatga
    24661 tttttctatc tccggccctt ccactattgt aaatcccatt cctatgaata tctgattgat
    24721 ttcatcctgc accttggtca ggggatgctt gtttcccagg gcttttcttt tggatggcat
    24781 ggtaacgtca atgaattctt ttcccagcct tatttccaag tccttccttt taaggcagta
    24841 tgcagtttct tcaatctttt cattgatata atcccttatt tcattggcca actgcccaat
    24901 tacaggcctt tcctcagggc tgagtccccc cattcccctc agtacctggg taagttctcc
    24961 ttttttgcca agatacttta ttctcacttc ttccagttcc ttctgggact gaacgctttc
    25021 cagctccttg acagaatgca atttgatctt ttcaagtgtc tctttcattt ttcatgctcc
    25081 tttcctatat gctatgtttt acaaaaatta aaaccttcat ccccaaaaaa gggacgaagg
    25141 ttatccgcgg taccaccctg cttgtttgca ttccgcaaac cgctccgaat ataacggtat
    25201 ttaccgtcaa agcctactgg tgctgttaac accatcttca gccctgaatc tccggagtga
    25261 acttcaactg cttcggtgcc ggtgcgctct cagccggtgg cagcacctct cttcaggcct
    25321 tccgcaattt actctctcca tcaacgattg cttcactatt atctttttga tgaatcttaa
    25381 tagattatat cataagcctt cacaggttac aacaaataaa actatatagt atataatatg
    25441 caatatattc attgattctc acaatatagt tgattataat ttaattaaag catagtgggg
    25501 gtgaggtcat gcttgacagg agaaagggtc ccgataagct tgtaaaatgg gtgaaatggt
    25561 caggaataat atcctggatc ctggttgcga tagtgctgtt tattactata attgcaaagc
    25621 ccagcttcga atcctacatg gacaagtcct ttcacgtaaa actacaaagc aattgggaca
    25681 ttaatctgat gcaatatgtc ctggtgctgc tggtgctgct tttcttcttc tgtataatat
    25741 ccattgcaat aaacctttcc aggtgcaggc gaaaaaccga caggttcaac aaaactctca
    25801 tactgaacgc tgttgcctct ttcataggaa ttctgcttta tctattgttt ttcatttaaa
    25861 gaaataggaa tgagcaatgt attattgttg gtcctctcat catccagcat cttcatggca
    25921 tattcctttg taaagcaggg ggctatgttc tcaagctttt taaagccctc ggtattcact
    25981 atgtcattga gtgcttcatt cctataccct gcaaaataca acatattcat tctgcatacc
    26041 gtctccagca ctgtttccct ttccgcatca gacagtccct caagctgttt aaggcattcc
    26101 tgatgcatcc tgccgcactg ttcggtgaaa aagctggctg aataatcttc gaaatttaga
    26161 ggagttccgt ctttcgcatt ctcatgtatg tcgtaatact ccatatccaa tgggattgta
    26221 cggtaatcaa agccaacata gggagtaaat ttcatccggc cgtactgatg gggataaact
    26281 gccaggctgc tggttccgat atcatagatt ctatttccct ggtattcatc ggatttaata
    26341 tcctgtatgt gaatatggcc ggtaaagact atttctattc catgcttgcg gaacactttc
    26401 aaagcatcct tgctgttttt tatagtataa tttgccttta tcatttcact gtggtcaatc
    26461 aggctgtgat gcataactgc tatcagcttc gcattattct ccttcgccag ggcggcgcac
    26521 tgctcaatcc atttaagagt atccggccgc agatatccgc tttgctcagg gtactttctc
    26581 gccatgttcc tgtcagtatc tgtggaatcc agcataagca gccaaatatc ttcagcaggc
    26641 atcgcaagat agcttaaaga attgggatcc cttgacacgc aaccctcgta gccgaagggg
    26701 tcataaagat tcacataatc ctctgaggta attgtttcga tgtctgttac ctcatctccg
    26761 atgtattttc tggcgtaagg attctgtata tcatgattgc ccggtacaac aaatacgcag
    26821 gtgcctatgt cctcaatagt tttcagcttt tctgccagtt caatatgact ctgcctggtt
    26881 ccattgcatg tcaggtctcc tgttataata agaaaatcgg ggttctccac cctaatatcc
    26941 ttaataagcg catcaagaag ctcatcagta tgattcagca ttttgccgtc ccccgaagcc
    27001 aaaaaatctt gaaaggcctt gccgttgtca taggtttcct ttgaaatata atgaggatct
    27061 gatacgataa aaaagctgaa agactgattg gtcttgatat cggcgcagcc aatagaatac
    27121 tgaaatgctt cggcacatcc cgaggatatg agaagtatca gaatatatgc aagcaaaatt
    27181 gaaaattgat gcttcttcat ggccaaccct ccctttcttt ccgaaaggag tgcaacgggt
    27241 tacaggttta ctttgttatt attatatcat acttttatta atatttgttt atttttaaac
    27301 aattattatt attgttattt tatagtaaaa ataccgcttt tcacttcaaa ctatccgctc
    27361 tcagccttat caaatacctt atcctcttca ctttcctctt cgtctataag ccctgcatct
    27421 tgttccaccg gagacggccg catcagatat aaggccatgg cggacagcag caccaccgct
    27481 atgcctgtta tcattagcag ccgcgtaccc cagccggagc ctttcttttt atcttcagtg
    27541 agtttgattt tgtccatact tcgcaggcct cctatcatgg tgcctggttt ttacatttca
    27601 gcctttccca tcattttacg tttctcatca gattcctgct tcatcaaaaa actaaaaaag
    27661 actatacgct tacaccttac atatagtctt tacattcatt atggtgggac cggctgggct
    27721 cgaaccagtg acccccacga tgtcaacgtg gtactctaac cagctgagct acggtcccat
    27781 ttactttcat attatatcat ttatgatttg cttgttcaag aagttaatga tgccagttcg
    27841 tataattttg ttcttataag ctgtacgtgg catatggttt caacaggctg gcacctaacc
    27901 tactctgacc ttgctcgtca gcattgcctt tcacctattc ttctctattt ccaatcgaac
    27961 ccataagatg aataatctcc tttaaatagc tgagttact
//
LOCUS       DAOOAB010000034        26449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2405_length_26449_cov_15.301318, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000034 DAOOAB010000000
VERSION     DAOOAB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 26449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26449
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_26449_cov_15.301318"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1154
                     /locus_tag="PKU88_08390"
     CDS             192..1154
                     /locus_tag="PKU88_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69331.1"
                     /translation="MNTIKPLYRIILPILILLSLLSFASCSPQKKPDLEKAQKEAEEK
                     KPPDELDKLKDSIKKVEKALEAIHEENKKPMFMQQEKIEKQASEEGKKQEGEQGGQSG
                     EQGGGGEGSSGGSESGGEQQKQQAQAPKLTPEQMKMKLEQEKYKKFESIKKDVLELHS
                     AWNSYEAKAISDFAMQTTINDFESALNNLTKTVEAQDAYLSLLEINQLYKYLPDFYML
                     YESKVPPDLDRLRFAAKKIMLLSEKQNFTGANDVLLYFENIWMTARPKLKSENAEIVS
                     KFEFAFADLKNAVIAKNDMIVEAKSEVLLKLVDEIEKKAEKSGK"
     gene            1347..2054
                     /locus_tag="PKU88_08395"
     CDS             1347..2054
                     /locus_tag="PKU88_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPX69332.1"
                     /translation="MKVLKLILPLINLLPESIIRLLGRIITGFLLSKYAELEIHGKEI
                     LAEREDKPTIYISNHLSNIDGVVLNSLLKNNSAAFMAGVKLEKNPFTRFFLKSINHIP
                     ITPGSPDKSAVKSALKHLGSGGSIVIFPEGTRSRTGSLIKVKKGFILLVKLAKVPVVP
                     IALEGTEIVLPINDNDMGSEKLRKAGVIVKIGEPFTLPGKNEHPGAEDWEEICVAYSM
                     KKIAAMLEPKYRGVYGN"
     gene            complement(2051..2521)
                     /locus_tag="PKU88_08400"
     CDS             complement(2051..2521)
                     /locus_tag="PKU88_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPX69333.1"
                     /translation="MSRKTMIMFSGDLDKAMAGLIIANGAAAMGDEVTMFFTFWGLNI
                     LRKAQKIKVRKSFMEKMFGFMMPRGADKLGLSKMNMGGMGTLMMKGIMRKKRVSSLPE
                     LIESAQDLGVKFIACTMSMDVMGIKPEELIDGVEFAGVATYLGEADEANVNLFI"
     gene            complement(2531..2755)
                     /locus_tag="PKU88_08405"
     CDS             complement(2531..2755)
                     /locus_tag="PKU88_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPX69334.1"
                     /translation="MAEKYINARGLQCPGPVVELFKVAKVSEKGDLITIEVTDMAFKK
                     DIAVWCRKTNNELVSLCEGEGTLKAVIRKG"
     gene            complement(2770..4449)
                     /locus_tag="PKU88_08410"
     CDS             complement(2770..4449)
                     /locus_tag="PKU88_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX69335.1"
                     /translation="MADNKLIVIGAVAAGTKAASKAKRDNPNLEVKVFTKERYISYAG
                     CGLPYYIGGIIRDKQELLVRSPEDFKREQGIDIFTEHEVKKIDASGKKVIVEDLKSGD
                     IREYSYDKLIIATGASPIVPPLENINLANIFTLRSVSDAFAVRELLDSGAAKKAVIVG
                     GGFIGLETAENLRNRGMDVTIVELVPHILPPFDEEMALYAQNHMIDRGVRILTGEKVT
                     GFEGSDGKVTAVKTSAGDIEADMVILSIGVRPNTGIAVEAGIETGAAGAIKVNKYMET
                     NIKDIYAAGDCAENTNLITGKPAWYPMGSTANKTGRIAGHNAALEKQEEALKESLEGV
                     LGTTIVRLFEVNAAKTGLSEKDALKEGYEAETVLVPANDTAHYFPGYRMINTKLVVEK
                     STHKVLGAQIVGEGIVDKPIDTIAAAITMGARAEDLAKLDLAYAPPFSMAVGSTILSA
                     NVMLNKLNKRMESVKAPVLPDMLEKGGINVLDVREEEEFFISSIPGSKNIPMGELEVR
                     LGEIDKNKETIILVCRIGNRAYMSYLKLKNLGFTNLKVLEGGICAYPYKLE"
     gene            complement(4467..4739)
                     /locus_tag="PKU88_08415"
     CDS             complement(4467..4739)
                     /locus_tag="PKU88_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPX69336.1"
                     /translation="MEDVKKYEGIAEILKSMAHPVRICIIKGLMEKKCNVTHMQECLE
                     LPQSTVSQHLGVLRAKGIIKGERNGLEITYSVVNEDVKKIINAIFD"
     gene            4901..5473
                     /locus_tag="PKU88_08420"
     CDS             4901..5473
                     /locus_tag="PKU88_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69337.1"
                     /translation="MINSIKGSPAVNQNYEAKEKHDNRQVENTKAKEDQAVILDLSKP
                     VEKKPTYTKPNAKNMDIKEINRLWKESQKSYETLRKLVEKLISKQGKKLEDLLEGKDV
                     LLVDEETRAAAAQAVSEDGELGVKAVSERIVAFAKEVSGNDKNRIGELKNAIIQGFKE
                     AEKALGGKLPDISQRTYDEVMRQLDEWEEA"
     gene            complement(5536..5754)
                     /locus_tag="PKU88_08425"
     CDS             complement(5536..5754)
                     /locus_tag="PKU88_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HPX69338.1"
                     /translation="MDTEYEEIIEYQEYSQKEKLKSKWARLESVQAKMKMTIKRFLKK
                     YGYPPDKMAKAVDIVMEQTKLMCQNESR"
     gene            complement(5808..11642)
                     /locus_tag="PKU88_08430"
     CDS             complement(5808..11642)
                     /locus_tag="PKU88_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HPX69339.1"
                     /translation="MKKIIRKKLLPLVMAVILTVGLLPQTVLAADPSGNWSDSGNYVE
                     PSNDGVTYTVTSAAELAWVAVQVNQGYSDFESCTIELSEELTEPIDLSEHYWTPIGSD
                     DGAFKGVFDGNLNTIANMTIGAFGDPANYDYLGLFGRTADAEIRDFALTDAVIYGDSQ
                     YTGSVVGYAAATEIFQCTVSGILYDYYDSSDLSGFGGLTGCAADRTVVTLCGMRGPLT
                     STSSSDDIRCVGGLIGLTKGYAAIANCYAECDINIERAYSNVGGFIGRAGNLTTITGC
                     WTYGGIQNDYEATVIGGFAGIISGEAGNGLSTVPVIENCFAEVYVDSTSVYAEVYGDS
                     TAQSSAGGFAGSVYLATIRNCCATDSVIGGTNSNVGGFIGLVRISLMENCYAAGSTAG
                     GIGSDVGAFIGHDEPIEDQFSYIYTSYWNSDAADEPVGYGYTTSYNLTELTTEEMQAS
                     DFADTLSQNAESIMDSTKTGSLDAWIAYGDDYPILDRGAWGKPLAIGVTASGTAGIGQ
                     TLTGSYTYYDADGDAESGTVVRWLYSTDPYGLSWFCLDGANGPSYTVTPDDAGHYFCY
                     EVVPKNGQSTGTAVRSGWIGPAEIGNWVDETEQPIRSGDSYIILSASNLAWVAEQVNS
                     GSKTFSGKSVVLARDIDLSGAYWTPIGTDSTNSFQGTFDGNDHVISGLMIGLENNPDD
                     TLEELGLFGYASGAAFQNVRIEDAAIYAENSKFGISFAACLVAYADNDTSVTDCSTSG
                     VISVGNRFCTGLLIGILTNSNSEITNCYTSGIISAGEQSGVGGFAGLIGESTNVVITN
                     CYAEGSAEGGDSTYVGGFISSLQTSSVINCSSTVDVTVGSNSYSGGLIAYCEDNDIIN
                     SYASGIATGGSNSDVGGFIGIDTAPSSVSLSFWNSSAADAAVGYGTGSYDITSMTVSA
                     MKSAVFASTLNSNVDTLSSYSLAQWSIIAGENDGYPVLEIESPGRWTDKGNYVPSAPT
                     NNGTIYYINTAQELAWVSAQGLSGNSFSGKTIELTADIDLSEHYWVPIASGYVASFEG
                     DFDGNGYTITGLTIGTAENPDYIYGDVGLFGSIDGSVIRNVTLENASVHLDCNSYVGG
                     LVGSAYISTISYCESEGVISVGGESLQNFTVGGLVGGARYTEIASCDSGGSVSGSGDN
                     RCSVGGLLGTAKTEVTVSNSGSSASVTNGGGAYTYAGGLVGLANEDITIEGCSASGNA
                     VGGDSCYAGGLIGNAYEGITIKNSHATGNAQGGGGDSRVGGFAALAYDDVTIDGCHST
                     GDASCGSNSAAGGFAGVLSETDVTNCYAIGGLSAGSSSYAGGFAGYVIDVAAINCYAT
                     GSIAGSGSAVGGFAGYTKTSEINNCYAAGSATGATYVGGFIGADYTATPSTVAYAYWN
                     SSAAQTAAGAARADADKIGIGAGISSYDMTAMTSDEMKASAFANSLNANVATLTEADS
                     SLSLSIWAAVTDSYPVLKKAPTATNVAISGSLKAGQTLTGGYTYYDADGDAESGSVYQ
                     WYIADDDNGTNAAAIDGATNNTYTLLSTDVGFYVRFAVTPSDGSVSGTAVMSAWAGPV
                     NSAASPGGGGSSDGGSGSGGGSSDGGSGSGSDDETEEINLTTSDKAVSVTAEMTSSSD
                     SVMNVIAREDFDKLAADSDGSVAITTAFATLVFDGVAVDYISAGSDTGDVTIEIKKGN
                     TTGLSDEDIEKLGDRPVYDFTVTVGNTQISELGGGCVTISIPCTLRDGENPNAVVVYY
                     IDKSGKLLVVQGAYNEETGTVEFTAYHFSMYAVGYNLISFSDVAGNAWYYDPVTFCAA
                     REITTGIGNGLFGPENTPTRGQFIVMLMRAYGIEADENASDNFSDAGNTYYTGYLAAA
                     KRLSIAAGVGNNMYAPEKEITRQEMFTLLYNALKVIGQLPESDSGKTLSDFSDAEEID
                     TWAVTAMTLLVETGTISGSGERLTPKGTTTRAELAQVLYNLLSK"
     gene            complement(11787..11921)
                     /locus_tag="PKU88_08435"
     CDS             complement(11787..11921)
                     /locus_tag="PKU88_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX69340.1"
                     /translation="MQALEKDELMVSQGEDYKDIAAKLGISENMVKHHVKRVVDKLHR
                     "
     gene            complement(12043..12681)
                     /locus_tag="PKU88_08440"
     CDS             complement(12043..12681)
                     /locus_tag="PKU88_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX69341.1"
                     /translation="MRIVIADDHALFADGLKNLLETHDYEVIGSARNGLEAYEMARLF
                     SPDIMLIDIFMPQCDGLEATTLISSSFPEIKIVILTSSENENDIFSAVKAGACGYFIK
                     SFESDKLFEVLRALENDEIPVSPGLAGKILKELPLGKMSLNETDRLTDRQCEVLSLVA
                     QGMFYRDIAEKLDISERTVKYHVKTSIDKLHLQNRNQLINFASRANIIEPSD"
     gene            complement(12814..14511)
                     /locus_tag="PKU88_08445"
     CDS             complement(12814..14511)
                     /locus_tag="PKU88_08445"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPX69342.1"
                     /translation="MGIYQFFPYIIFSSFSFATLIILSIYGIQYRHHVGVKEFIMVML
                     ASAWSVFCQTFELIGTTLPVKLFWADMEYLGMMFSAFAFLLMAIRFAGYDRYITKRNV
                     LIVTLVYTIFWALVLTDPYLGLMRTNFSLDTSAIPYTIKKDYGLIYPLYTLFIYSIII
                     ASFSILVNVARRKDSLYRKQAVVLILYIVLMVFPNVGFYFQLLPITRYDLSPAFFGPA
                     SLLMAWSIYRHKFLNIMPIARDLLIETMNSGVIVTDGDKKIIDINQSAIELLSLEGAD
                     MLSRDISEIQPVSSLLTDSEEEQQAAFAFDNGNGKRVYEMKSQTILNKQGESTGRLIV
                     LDDVTEQRENTQKIITQQNTLSVMRERERLGRELHDGLGQVFGYINAQAQTVREYMCQ
                     GKPDQAVKRLDELIAATRDAHGSIREYILEMRGISPRNRSFSAVLKQLAYEFSEKNGI
                     SVNIIFDDNLPESFPEDEKAINLLKIIQEALTNIRKHAGACQVEVSFQKEDGYAVICI
                     ADSGAGFDPDSQSDGHHYGLSIMRERAKEMGADLWISSQIGRGSRVTLRLPHRGEEV"
     gene            complement(14711..14935)
                     /locus_tag="PKU88_08450"
     CDS             complement(14711..14935)
                     /locus_tag="PKU88_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69343.1"
                     /translation="MEGQAFPLGELTADILNITPEELAFTFQMIENAVLKAKEKALVR
                     LPDVCLNGELGVWREVRNMMRAALRGQKAP"
     gene            complement(15598..16746)
                     /locus_tag="PKU88_08455"
     CDS             complement(15598..16746)
                     /locus_tag="PKU88_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPX69344.1"
                     /translation="MKTRKLLMIPGPTPVARSIQEQMGRETVAFGDPAFVKDFKELLE
                     DLKSLFNCSGEVFVVAGTGTMGMEMAIANTTKAGDNVLIVSHGFFGDRFIDLCSRKGL
                     NADVISSEWGETVPVEKMKEKLKEKKYAAMTVTHVDTSTGVCAPVAEIGKMMKEFPET
                     IYIVDGVCATAGEPEDVDAMGIDILFTGSQKAFGVAPGLTMLWASGKALERRKSLGSI
                     PEFYIDFEKWLPVMKDTAKYFATPAINMIWALKESVRIIKEEGIENRYARHRKTGTAM
                     QAALEALGFRILAKEGCRAATLSNPVYPEGVDDAKFRGLLAEEGIMVAGGLAAYAGRM
                     FRLGHMGNIDKHDMVSVIAAVERALYRAGMPVELGKGVGVVQQHLIDS"
     gene            16930..18717
                     /locus_tag="PKU88_08460"
     CDS             16930..18717
                     /locus_tag="PKU88_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX69345.1"
                     /translation="MSYLKLIDDKIQSLAESLSSILNVETTVVDEHLLRIAGTGDFYH
                     RINESSPENSLFAKVLKTGKADLNLYDKKNPICQTCNNSDTCKELKSMILPIKVENDV
                     IGVVSFVSFNAAQDQLMVEKKEEYLEILKHFAETIEKEIISIKMVNKLNMGIAEINGI
                     INSINRSIIILNKEQRIIYINSKAIKSLNINFSADKIINKRIGSIIKGFKAEDTNNKE
                     VIGNWAIGGKLIKVIYKVNYIILDNKNVSTIINFDTLDEIISIAMTYNSDEETTFENI
                     IGRSQIMQDVIEKAKIAAKSDSTIFLHGTSGTGKELFARSIHNASYRKDGPFVAVNCA
                     ALPENLIESELFGYEKGSFTGASQKGKTGKFEQANNGTLFLDEIADLPLHLQAKLLRV
                     LQDKKIDRIGGSAQINVNVRIISATHKNLEEMVRNNEFREDLFYRLNVIPLHLPSLKD
                     REEDVLLCSEYIIKKLCLKMNKSPKRISKDVETMFMGYGWPGNVRELENVLEYAINFS
                     FDDEITAENLPEYFLANYAKKDNDEYINIESPEIDNLKSLGDITKEYEKKLIRKLISK
                     YGNTTETKRKIAEKLGMSITTLYRKLNDY"
     gene            complement(18725..20002)
                     /locus_tag="PKU88_08465"
     CDS             complement(18725..20002)
                     /locus_tag="PKU88_08465"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPX69346.1"
                     /translation="MLSKKVMENYILILKSELIEALGCTEPIAIAYAAAKARSVLGKA
                     PERIEVGCSGNIVKNVKGVVVPNTGGLIGIDAAAVAGVVGGDPDRGLQVLESVKRKQY
                     CTVKRLTEEGYCKVYHLKGVDNLYIEAKVYAGNESAEVYIVDAHTNIVKIVKDGKVLF
                     ENNNGGRKDSKLAGDKTLLNIKDILEFAETVDLDILHELLEKQVTVNTAIAEEGIRND
                     YGVSVGKTLLENYGNDVTVRARAMAAAGSDARMSGCSLPVVINSGSGNQGITVSVPVI
                     EYARELKTSEEKLFRALIISNLVSIHQKAQIGNLSAYCGAVSAAAGSGAGIAYLNGGS
                     YKEISSTIINTIANIGGMVCDGAKPSCAAKISSAVGAAILGYQLSARGKVFRSGEGLV
                     KGGVEETIKSFGRVGKKGMEGTDIEIINIMLDK"
     gene            20302..21087
                     /locus_tag="PKU88_08470"
     CDS             20302..21087
                     /locus_tag="PKU88_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPX69347.1"
                     /translation="MDNYNEKNTKTIGSVIKAIEILEELAESEKGLGLTEISSSLNYG
                     VSATYHLLNTLKLHNIIEQNKKTKKYRIGVGLFRISSKAKSQYSLANLAMPFLDKLRA
                     TVDETSNLLVLDGYEVLYIAQSESTKLLKMFTQLGAKAPLYCTGGGKAILAYKPLSFQ
                     ELALSKINFIKYTENTIVTADGLRKEFETIKKQGYSIDNEEREEGITCAAAPIFDCYG
                     EVIAAVSISGPSYRIKEKDSKSIISSVVLTAKEISANLGYMEK"
     gene            complement(21092..22042)
                     /locus_tag="PKU88_08475"
     CDS             complement(21092..22042)
                     /locus_tag="PKU88_08475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPX69348.1"
                     /translation="MKEVTFDDYRQHYRYKYVVENIKDVIWEMDENLIFTYISPSCKE
                     MSGYESSEMIGKRMTDFLVPESRNYVLDQWKKNAHSRKSCNSRGIIMYDLQFICKDKG
                     LIWVEVSVKPVFEHDRFLGYIGVTRDVTEKKVHENELKKYYNELETANRELEKLATFD
                     MLTGAYNRRRFEEYVKSAVDMKEMHGSPFSIIMFDIDCFKLINDIYGHSWGDRILQVV
                     TEIVKASIRETDKLFRWGGDEFIILMPGKASKDAYKVAERVRKSIEKNNFGIQHDRIT
                     ISTGVGEYITDESIDQFVSRVDDALLTAKSIGRNKVELCI"
     gene            complement(22181..23122)
                     /locus_tag="PKU88_08480"
     CDS             complement(22181..23122)
                     /locus_tag="PKU88_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPX69349.1"
                     /translation="MVGGIDKYILGISEIFDAPTEDKGSRISLTEHRDVRNIIKKRER
                     DSHKGTYGRVGILSGSKGMAGAAVLNCNAALRSGSGLVKACVTEAIYCIVESMALEAI
                     TYAFNEQNINYDELNRELIQFSDVIATGSGCADMKRYGELLKFLAENSQKPLVIDAEG
                     INLLTPDILNNHRQIMILTPHYGEMARLINKDPDYMRENIIEKAAEFACRYNVYLVLK
                     GARTVIACPRGNSYINTTGNPGMATAGSGDVLTGIIASLIGQKIEITDALRAAVYIHG
                     LAGDIGSSYAGEYSLIAGDIIRYLPEAFKAVMGQEDV"
     gene            complement(23157..23933)
                     /locus_tag="PKU88_08485"
     CDS             complement(23157..23933)
                     /locus_tag="PKU88_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HPX69350.1"
                     /translation="MEFEITVLNVVFLCLAGFAAAAVDAIAGGGGLISLPAIMAAGVP
                     PHFALGTNKFSSSFGAVTSAYTFIRSGKVHIPLMKFTVPCTLAGSVIGVFTALRIDQK
                     FLRLIILVLIFATAIYTIAKKDFGSEDKFKELTGRNIIKGCLFAFSLGFYDGFFGPGT
                     VSFLIFLFIKVYGFDFTIAAGNSKVLNFVSNFTSLVMFAINGKIYYMLGIPIGLAMIA
                     GARMGSKIAIKNGAKVIKPIFVTIALALTLKLLFDAVRLL"
     gene            complement(24001..25647)
                     /locus_tag="PKU88_08490"
                     /pseudo
     CDS             complement(24001..25647)
                     /locus_tag="PKU88_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611065.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
     gene            complement(25638..26339)
                     /locus_tag="PKU88_08495"
     CDS             complement(25638..26339)
                     /locus_tag="PKU88_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69351.1"
                     /translation="MGYSSNMTVLGSVFPDTVIKCSLTRDQTHNVGWGLYKYLKGHGN
                     EYLEFARAMATHTVNPRGLDFYGDEEYGDGYKGYCFQKAAAIEKEVIEACNIPAEFGL
                     WKAHNFIEMGIEMNIAESERELMDVFHKGLTDYCLIKELSLYLDRYYGLKEKSVSDCF
                     CKFTEFMELKDLNSFTLARRYNTQMNYKHGIEIDISKCSNIIDKARGIVQEDFESFIS
                     YCAEKVKASLKEECT"
BASE COUNT         7095 a         6570 c         5299 g         7485 t
ORIGIN      
        1 ttcagcggga caggagaaac gtccccgtgt cccaggagaa acgtccccgt gtcccaccag
       61 tgtcctcaaa aaaattttca gttgtattcc ataaatggaa agatatttag ttgtcattaa
      121 ggactcaaaa ccgcttctta aattatatta aagaataatt aaggaataat aaagttaaag
      181 aggtgatttc tttgaatacg ataaagcctt tatatagaat aatacttccg atacttattt
      241 tattgtcctt attaagcttt gcttcatgtt ccccccagaa gaagccggat ttggagaagg
      301 cgcagaaaga agcggaagaa aaaaagcctc ctgatgaact ggataagctt aaggacagca
      361 taaaaaaagt ggaaaaagcc cttgaggcaa tacatgagga aaacaaaaag ccaatgttca
      421 tgcagcagga aaaaattgag aagcaagctt ccgaagaggg caagaagcag gaaggggagc
      481 aaggaggcca gtccggtgaa cagggcggag gcggcgaagg cagcagcggc ggcagtgaaa
      541 gcggcggcga gcagcagaag cagcaggctc aagcccctaa gctcactccc gaacagatga
      601 agatgaaact ggagcaggaa aaatacaaaa aatttgaaag cattaagaaa gatgtattgg
      661 aactgcatag tgcctggaac agttatgaag caaaagcaat atctgatttt gcgatgcaaa
      721 caaccattaa cgactttgaa tcggcactta acaatctgac caaaacggtt gaagcccagg
      781 atgcctatct gagcttgctg gaaataaatc aactatataa atacctgccg gatttctaca
      841 tgctctacga gtcaaaggtt ccccccgacc tcgacaggct taggttcgcg gcaaagaaaa
      901 taatgctgct gagtgaaaag caaaatttca ccggtgcaaa tgatgtactt ctgtactttg
      961 aaaatatatg gatgacagcc aggccgaagc tcaaaagtga aaacgctgaa attgtcagca
     1021 agtttgaatt tgcctttgcc gatttgaaaa atgccgttat cgcaaaaaat gatatgattg
     1081 ttgaagcaaa atccgaagtc ctgctgaagc ttgttgatga gatagaaaaa aaggcggaaa
     1141 aatccggcaa atgataagaa cccgcttaat tcggggttct tatcatttta tatataatgt
     1201 gatataatat gcgcaaacgc gtattatatt tcttatttaa ttgccgcgcg agtatttcag
     1261 cttcgctgaa aacgcgcatg gaacaataat aaataccgct tatttaatgg tcatcagaca
     1321 ttgagtttgc ggaccgggag taacatatga aagtgttaaa gcttatcctg ccgttgataa
     1381 atcttttgcc cgagtccata atccgcttgc tgggaagaat aatcaccggt tttttattgt
     1441 caaagtatgc ggaactggag atccacggca aagaaatatt agctgaaaga gaagacaaac
     1501 caacaatata tatatcaaat catctgagca atattgacgg cgtcgtgctg aacagcctgc
     1561 tgaaaaacaa ttcggctgct ttcatggcag gagtcaagct ggaaaaaaat cctttcacac
     1621 gctttttcct taaaagcata aaccatatac ccattactcc gggcagcccc gacaaaagtg
     1681 ctgtgaaatc tgccctgaag catctcggct ccggaggctc aattgtcatt ttccccgagg
     1741 gtacccgcag caggacaggc tcccttataa aagtaaagaa gggtttcatc ctcctggtaa
     1801 agctggcaaa ggtgcccgta gtgcccattg ccctggaagg aactgaaata gttcttccca
     1861 ttaacgacaa cgacatgggc agtgaaaagc tccgcaaggc cggagtcata gtcaaaattg
     1921 gggagccttt tacccttccc gggaagaatg aacaccccgg ggctgaagat tgggaagaaa
     1981 tatgcgtcgc ctactcaatg aaaaagattg cggctatgct ggagccaaaa tacaggggag
     2041 tatatggaaa ttaaataaac aagttaacgt ttgcctcatc cgcttctccc aggtacgtgg
     2101 cgacccctgc aaattccact ccgtcaatca gttcctccgg ctttataccc attacgtcca
     2161 tagacatggt gcaggctatg aacttcacac ccaagtcctg tgcactctca ataagctcag
     2221 ggaggctgct tactctcttc tttctcatta tacccttcat cattaatgtg cccataccgc
     2281 ccatattcat ctttgagagc cccagcttgt cagctcccct gggcatcata aatccgaaca
     2341 tcttttccat aaaggacttc ctcactttaa tcttctgcgc tttcctcaaa atattcaggc
     2401 cccagaaggt gaaaaacatt gtcacctcat cacccatggc agcagcaccg ttggcaataa
     2461 taagccctgc catggcttta tccagatccc cgctgaacat tatcattgtt ttcctgctca
     2521 taatcgtacc ttaccctttc cgtatcactg ctttgagtgt tccttcccct tcgcaaagac
     2581 taaccagctc attattggtc tttctgcacc agaccgcaat atctttcttg aaagccatgt
     2641 cggtaacctc tattgtaatc aaatcacctt tttcagatac ctttgcaacc ttgaaaagct
     2701 caactacagg ccccgggcat tgaagccccc ttgcattaat gtatttttca gccatttgta
     2761 tctcctcctt tattccaact tatacggata agcgcaaatt ccgccttcca gaactttcaa
     2821 atttgtaaaa ccaagattct taagcttgag atatgacata tatgctctat tacctatcct
     2881 gcatactaaa attatcgttt ctttattttt atcaatctct cctaatctaa cttcaagctc
     2941 acccatagga atgttctttg aacccggaat tgaagatatg aagaattcct cctcttctct
     3001 cacatcaagc acattgattc cgcccttctc cagcatgtcc ggcagaaccg gtgctttaac
     3061 ggactccatc cttttattca gcttgttcag catgacattg gcagacagaa tcgtcgaacc
     3121 tactgccata gaaaaaggcg gtgcgtaagc aagatccagc tttgccaggt cttctgccct
     3181 tgctcccata gttattgccg cagcaatagt atctatgggc ttatcaacta taccctctcc
     3241 tactatctgt gctccaagca ccttatgagt acttttttcc actacaagct tagtatttat
     3301 catcctatag cccgggaaat aatgagctgt gtcatttgcc ggaacaagta ccgtttcagc
     3361 ctcatagcct tcctttagtg catctttttc cgaaagcccc gtctttgcgg cattaacttc
     3421 aaacaacctg acaattgttg ttccaagcac tccctccagg ctttccttca gagcttcctc
     3481 ctgcttctca agtgccgcat tatgccctgc tatccttccg gttttgtttg ccgtagaacc
     3541 cataggatac catgcaggct ttccggtaat cagattcgta ttctcagcgc agtctcccgc
     3601 tgcatatata tccttgatat tcgtttccat atatttgttc accttgattg ccccggctgc
     3661 gcccgtctct atgcctgctt caacggcaat acccgtgttt ggccttactc cgatagacaa
     3721 tatcaccata tcggcttcaa tatcccctgc cgaggtttta acagcggtga ctttcccatc
     3781 gctgccctca aagccggtga ctttttctcc ggtcagtatc ctgactcccc ggtctatcat
     3841 atggttctgt gcatataacg ccatctcttc atcaaaagga ggcagtatgt gaggtacaag
     3901 ctcaacaata gtaacatcca tgcccctgtt tctcaggttc tcggccgtct ccaggccgat
     3961 aaagccgccg ccgactatta ccgccttttt tgcggctcct gagtcaagca gttctcttac
     4021 tgcaaatgca tcggatacgc tcctgagtgt aaatatgttg gcaagattta tgttttccag
     4081 gggcggtaca atcggggaag ctccggtggc tattatcagt ttgtcatagg agtactccct
     4141 tatatccccg cttttaaggt cctcaacaat tacctttttc ccggatgcat ctatcttctt
     4201 tacctcgtgc tcagtgaata tgtctattcc ctgctctctt ttaaaatcct cgggggacct
     4261 gacaagcagc tcctgcttat cccttataat tccgcctata taatatggca ggccgcatcc
     4321 ggcataagaa atatatcttt ctttagtaaa gaccttaacc tccaaattgg gattgtccct
     4381 ctttgccttt gaagccgcct ttgtacctgc tgcaactgca cctattacaa ttagtttgtt
     4441 atcagccatg gtttacctcc ttaaatttaa tcaaatattg cattgatgat ttttttcaca
     4501 tcttcattta ctacactgta ggttatctcc agtccgtttc tttctccctt tattattccc
     4561 tttgccctca gaacacccag atgctgggat acagtggact gcggaagctc caggcattcc
     4621 tgcatatgtg ttacgttgca ctttttttcc ataagcccct ttataatgca tatccttacg
     4681 ggatgggcca tggatttcaa tatttctgca ataccttcat acttttttac atcttccatt
     4741 aagtcacctc cgtctatatt gctatattct aatattacga tattgtgata ttattgtcaa
     4801 gtataagcag caatttttat taaatttctc atatcttctc tatattcatc ctatatatat
     4861 atcataagga tgtaaattta tttaccaagg agggaatata atgattaaca gtattaaagg
     4921 aagtcctgca gttaatcaaa attacgaggc aaaagaaaaa catgacaata ggcaggttga
     4981 gaatactaaa gcaaaagaag atcaagcagt aattctggat ttaagtaagc ctgtcgaaaa
     5041 aaaacctact tataccaaac cgaatgcaaa aaacatggac ataaaagaga ttaacaggct
     5101 ttggaaagaa tcgcaaaaat cttacgaaac attgcggaag cttgttgaaa aactcatttc
     5161 gaagcaaggt aaaaagctgg aggatttact ggaaggaaaa gatgtcctgc ttgtggacga
     5221 ggaaaccaga gctgcagccg cacaggcagt ttccgaagac ggagaattgg gagttaaggc
     5281 agtcagcgaa agaatagttg cctttgcaaa ggaagtttcc ggcaatgaca aaaacaggat
     5341 cggcgagtta aaaaatgcta ttatccaggg cttcaaggaa gcagagaaag ctcttggggg
     5401 caagctgccg gacatttctc aaagaaccta cgacgaggtc atgaggcagc ttgatgaatg
     5461 ggaagaagct taaacataga ctggtattaa agggtcaggc atggaatttt tccgcctgac
     5521 cctgtttgta ttaccctatc tgctctcatt ctgacacata agtttagtct gttccattac
     5581 aatatccacc gctttagcca tcttatcagg tgggtatcca tatttcttaa gaaagcgttt
     5641 aatcgtcatc ttcattttag cttggacact ctcaagcctt gcccatttgc tcttaagctt
     5701 ttctttctga ctatactctt gatactcgat tatttcctca tattccgtat caatcttcgg
     5761 tttcagttct tccggcttct acatcctatg cttgaaaatt tctgcattta tttggaaagt
     5821 agattatata gcacctgcgc cagttctgcg cgggtggtcg tgccctttgg cgtcaacctt
     5881 tctccgctac cgctgattgt tccggtctca accagcagcg tcatggcagt tacagcccaa
     5941 gtgtctatct cttctgcgtc gctgaaatcg gacagtgttt ttccggaatc gctttcgggg
     6001 agttgcccaa tgaccttgag agcgttgtaa agcaaggtga acatctcctg tcgggtgatt
     6061 tccttttccg gcgcatacat gttattgccc actcctgccg cgatgctaag ccgcttggct
     6121 gccgcgagat agcctgtgta gtatgtattc ccggcgtcgc tgaagttatc ggatgcgttt
     6181 tcgtcggctt caattccgta ggcgcgcatg agcatgacga taaattgtcc gcgcgtaggc
     6241 gtgttttccg gcccgaacaa gccgtttccg attccggtgg ttatttcgcg cgcggcgcag
     6301 aaggtaacag ggtcatagta ccaggcgttg cccgcgacgt cggaaaagct tatcaagtta
     6361 tagccgaccg cgtacatgga aaagtgatac gccgtaaatt caacagttcc ggtttcctca
     6421 ttatacgcac cctgaaccac cagcagctta cctgatttgt ctatgtaata gaccacgacg
     6481 gcattggggt tttcaccatc ccgcagcgtg catggtatgc tgattgtcac acatccgccg
     6541 ccaagttcgg aaatctgtgt attaccaacc gtaacggtaa agtcgtacac cggacggtcg
     6601 ccgagctttt cgatatcttc atctgacaga ccggtcgtgt tgcctttctt gatttctata
     6661 gtcacatcac ccgtatccga ccccgcgctg atataatcta cggcaacacc gtcaaagacc
     6721 agcgtggcaa aggctgttgt gattgctacg gagccatccg aatcggctgc aagcttgtcg
     6781 aagtcctctc ttgcgattac gttcatcacg gaatcagatg agcttgtcat ttccgccgta
     6841 acgcttacgg ctttgtctga cgtggtcagg tttatttctt ccgtctcgtc gtcactaccg
     6901 ctgccggaac ctccgtctga gctgccgcct ccgctgccgg aaccgccgtc tgaactgccg
     6961 cctccgccgg gagaagccgc gctattcaca ggacctgccc aggcactcat gacagctgtg
     7021 cctgaaacgc tcccgtcaga cggcgttacc gcgaagcgga catagaagcc gacatctgta
     7081 gaaagcagcg tgtatgtatt gttagtcgcg ccgtctatag cggcagcgtt cgttccgtta
     7141 tcatcgtcgg caatatacca ctgatatacc gagccagatt cggcgtcccc gtcggcgtcg
     7201 taataggtgt agccgcccgt gagagtctgc ccggccttta aagaccctga aatggcgaca
     7261 ttcgttgccg taggcgcttt tttgagtaca ggataactgt ctgttacagc cgcccatatg
     7321 gaaagcgata aactgctgtc ggcctctgtc agcgtggcga catttgcatt cagcgaattg
     7381 gcaaaggcgg aagccttcat ttcatcggat gtcatggcgg tcatgtcata cgaactgatc
     7441 cctgcgccga tgccaatttt gtcagcatcg gcacgcgcgg ctcctgctgc tgtctgagcc
     7501 gccgagctgt tccaatatgc gtaagcgacc gtgcttggag ttgcggtgta atccgcaccg
     7561 atgaagcccc cgacatatgt cgcccctgtc gccgaacctg ccgcgtagca gttattaatt
     7621 tcgcttgttt tcgtgtatcc ggcaaagccg cccactgcgg agcccgatcc ggcaatactt
     7681 cccgtggcat agcagttgat tgctgctaca tcaataacat agccggcgaa accgcctgca
     7741 tatgagctgc ttcctgcgct taaaccgcca atggcatagc aattagtaac atcggtttct
     7801 gacaaaacac ccgcgaagcc gcccgccgcg gaattacttc cgcaggatgc gtctccggtg
     7861 gaatggcagc catcaatcgt cacatcgtca tacgcaaggg cggcaaaacc gcccacgcgg
     7921 gaatcgccgc cgccgccctg agcattgcct gtggcgtggc tgttcttgat ggtaattcct
     7981 tcgtaggcgt ttccgatcag cccgcccgca taacagctgt cgccccctac agcattgccg
     8041 gaagctgagc agccttcaat agtgatatcc tcattggcaa ggccgacaag tccgcccgca
     8101 taagtatacg cgccgccgcc gtttgtaacg gaggccgatg agccgctgtt gcttactgtc
     8161 acttcagttt tcgctgttcc gagaaggcct cccacgctgc acctgttgtc gccgcttccg
     8221 gaaacgctgc cgccggagtc gcagcttgcg atttccgtgt accgcgctcc tccgaccaga
     8281 ccgcctacag taaaattctg taagctttcg ccgccgacag aaattacacc ttccgattca
     8341 caatacgaga tcgtgctgat ataagcgctc cccacgagac cgcccacata ggaattgcaa
     8401 tcaagatgga cggacgcatt ctccaacgtg acattgcgta ttacggaacc gtcgatcgag
     8461 ccgaacaaac caacgtcgcc gtaaatatag tcgggattct ccgctgtgcc gatggtaagc
     8521 ccggttatgg tatagccgtt tccgtcaaaa tcgccctcga aggacgcgac ataaccggat
     8581 gcgatgggga cccaataatg ctctgaaagg tcgatatctg cggtcagctc aatcgtttta
     8641 cccgaaaacg aattgccgct caatccctga gccgaaaccc aggcaagctc ctgtgccgtg
     8701 tttatgtagt agattgtgcc gttattcgtc ggcgctgagg gaacgtaatt gcccttgtcg
     8761 gtccatcgcc cggggctctc aatttccaaa acaggataac cgtcgttttc gccggcgata
     8821 atgctccact gcgcgaggga ataactagac agcgtatcga cgtttgaatt cagcgttgag
     8881 gcaaacaccg ccgatttcat ggcgcttacg gtcattgatg taatatcata agaaccggtc
     8941 ccatagccga cggcagcatc ggccgcggag ctgttccaaa atgaaagcga tacagacgat
     9001 ggagctgtgt caatcccaat aaatccgcct acatcggaat tgctgccgcc ggtggctatc
     9061 cccgaggcat agctatttat tatgtcgtta tcttcgcaat acgctataag tccgcctgaa
     9121 tatgaattgc tgccaactgt cacgtcaaca gttgaagagc aattaatcac cgagctcgtt
     9181 tgcaatgagg agataaatcc gcccacgtat gtagaatcgc ctccttccgc gcttccctcg
     9241 gcatagcagt ttgttataac aacgttcgta ctttcaccga tcaagcccgc aaatcctcca
     9301 actccggatt gctctcctgc tgatatgatt ccagatgtat agcaattcgt gatttccgaa
     9361 ttagaattgg taaggatccc tatcaaaagt cccgtacaga aacgattccc gaccgaaatg
     9421 acgccggacg tggagcaatc cgtgaccgaa gtgtcgttat ctgcgtatgc cacaagacag
     9481 gccgcgaagc taatcccaaa tttgctattt tccgcataga tcgccgcgtc ctcaatgcgt
     9541 acgttttgaa acgccgctcc ggaagcgtag ccgaagagcc ccagctcctc cagcgtgtca
     9601 tccgggttgt tttccagtcc gatcataaga ccggaaatga cgtgatcgtt cccgtcgaaa
     9661 gtgccctgaa aactatttgt ggaatcagta ccgattggcg tccaataagc gccggaaagg
     9721 tcaatatccc gcgccagcac gacggatttt ccggagaagg ttttgctgcc gctgtttacc
     9781 tgttcggcga cccaagcaag attcgaggcc gacaagatga tataggagtc accgcttctg
     9841 ataggttgtt ccgtctcatc tacccagttc ccgatctccg ccgggccaat ccaaccgctt
     9901 ctgaccgcag tccccgtgct ttgcccgttt ttggggacca cctcatagca gaaataatgt
     9961 ccggcgtcat ccggggtcac ggtgtaggac gggccgttcg cgccatctaa acaaaaccaa
    10021 gacaaaccgt aggggtcggt cgagtacagc catctcacga cggtacccga ctcagcgtcc
    10081 ccgtcggcat cgtaataggt atagcttccc gtcagcgttt gacctattcc ggctgttcca
    10141 gaggcagtta cgccgattgc cagcggcttt ccccacgccc ctcgatcgag aatgggataa
    10201 tcgtccccat aggctatcca tgcatccagc gagcctgttt ttgttgaatc cataatggat
    10261 tcggcgtttt gacttaatgt atcggcaaag tccgaagcct gcatttcttc cgtcgtcaac
    10321 tcagtcagat tgtaggatgt tgtatatccg taacccacgg gttcgtcagc ggcatcgcta
    10381 ttccaatagc tggtgtatat gtatgagaat tgatcctcga taggctcgtc atgaccgatg
    10441 aaggcgccta catcggaacc gattcctcct gccgtgcttc ccgcggcgta acagttttcc
    10501 atcaacgaaa tgcggacaag cccgataaaa cctccgacgt tggagtttgt gccgccaatc
    10561 acgctgtccg tggcgcagca gtttctgatc gtcgccagat atacgcttcc ggcaaagcct
    10621 ccggcggagg actgagctgt actgtcacca tagacttctg catatacgct tgtactgtca
    10681 acatagactt ccgcaaagca attttcaatt accggtactg tggaaagccc gttgccagcc
    10741 tcgcctgata tgattccggc aaagccgccg ataacggtgg cttcataatc attttgaatt
    10801 ccgccatacg tccagcagcc tgttattgtg gttaaattgc ctgcgcgtcc aataaagccg
    10861 ccgacattag aataagctct ctcgatattt atgtcgcact cggcgtaaca attggcaatc
    10921 gctgcatatc ctttggtcag accgattaag ccgccgacgc agcggatatc gtcggatgat
    10981 gatgttgacg ttaggggtcc ccgcataccg cacaatgtaa cgactgtgcg gtccgccgcg
    11041 cagccggtca gaccgccgaa gccggacagg tcggaggaat cataatagtc atataaaata
    11101 ccactcacgg tgcattgaaa aatttcagtg gctgccgcat agcccacaac gcttcccgta
    11161 tattgggaat cgccatatat cacggcatct gttaaagcga agtctctgat ttccgcgtcg
    11221 gcggttcttc caaacagccc taagtaatca taatttgccg ggtcgccgaa ggctccgatg
    11281 gtcatgttgg cgatcgtatt taagtttccg tcgaagacac ctttgaatgc cccgtcgtcg
    11341 cttccgatcg gcgtccagta gtgctccgaa agatcaatgg gttccgtgag ctcttcggaa
    11401 agctcaatcg tacagctttc aaaatccgaa tacccctggt ttacctgcac cgccacccag
    11461 gccagttcag cggcgcttgt gaccgtgtag gttactccgt cgtttgacgg ttccacataa
    11521 ttgccggaat cactccagtt gcctgacgga tcggcagcca gtacggtctg cggcagcaat
    11581 cccaccgtca gaatgaccgc cataaccaag ggcaataact tttttcttat gattttcttc
    11641 attctatcag ctcctgtcat ttgttattta cggcagagaa ctctaccaaa ttgcgacata
    11701 aaacacttgt ccagcatcaa ttataaagaa aagatctttt tggcacactg tccttgagga
    11761 cagggagggt gaaaaaatgc tgttttttac cggtgcagct tgtcgacaac ccgcttaaca
    11821 tgatgtttaa ccatgttctc agagattcca agcttagcgg caatgtcctt gtaatcttcc
    11881 ccctgtgata ccataagctc atctttttca agcgcctgca acctcaattg cgccaaaata
    11941 cgactatttg taatgaatta taaataaaaa aacttcgcgg cgtactgtcc ttgagaacag
    12001 gtgcccgcga agttttttaa gatttttgta tatttatcgc cgtcaatctg acggctcgat
    12061 tatgtttgcc cttgacgcaa agttgatgag ctgattgcgg ttttgcaggt gcagcttatc
    12121 tatggaggtt ttgacatggt actttaccgt ccgttcgctg atgtccagct tctccgcgat
    12181 atccctgtaa aacataccct gcgccacaag ggacaacacc tcgcattgcc ggtcggtcag
    12241 ccgatcggtc tcatttaagc tcatttttcc cagcggtagc tcttttagaa ttttccccgc
    12301 cagaccgggc gaaaccggaa tttcgtcatt ttcaagggcc ctgagcacct cgaaaagctt
    12361 atcgctttca aaacttttaa taaaatatcc gcaggccccg gcctttaccg cgctgaatat
    12421 gtcgttttca ttttcggatg aagtcaggat cacgattttg atttcaggga agctggagga
    12481 aatcagcgtc gtggcttcca gcccgtcgca ctgcggcata aatatgtcga tcagcataat
    12541 atcgggagaa aagagccgtg ccatttcata agcctcaagc ccgtttctgg cgctgccgat
    12601 gacctcataa tcatgcgttt caagcaggtt tttcagcccg tccgcaaaga gcgcatgatc
    12661 atccgctatg acaattctca tcttatgacc attcctttcg cttgacttca gacacaaaac
    12721 gaccatgaaa aggaatggtc ataatgtgcg cgttttgcgg ttgcccttag ggcgagcaaa
    12781 acgtacttga aataaacgaa gtttgaactt gtttcaaact tcttcacctc tgtggggcag
    12841 ccttaacgtt actctggatc cccttccgat ctgcgaggaa atccagaggt cggcgcccat
    12901 ttctttggcc ctctccctca tgatggaaag gccgtaatga tgaccatcac tttgagaatc
    12961 gggatcgaaa cctgcgccgg aatccgcaat gcaaatgacg gcatagccgt cttccttttg
    13021 gaaagaaacc tccacctgac aggccccagc gtgctttctg atattggtca gcgcctcctg
    13081 tattattttc agcaagttga tagccttttc atcctccgga aagctttccg gcaaattatc
    13141 gtcgaatatg atattgacag aaatgccgtt tttctcgctg aattcatagg caagttgttt
    13201 gagaacagct gaaaagcttc tgttgcgggg agaaattccg cgcatctcga gtatatattc
    13261 ccgaatgcta ccatgggcat ctctggtcgc ggcaatgagt tcatccaaac gtttgaccgc
    13321 ctggtctggt tttccctgac acatatattc tctaactgtt tgcgcttgcg cgttgatata
    13381 gccgaacacc tgacccagcc cgtcgtgcag ctcgcgccca agccgttcgc gttcccgcat
    13441 gacgcttaaa gtattttgct gagtgatgat cttttgtgtg ttctcccttt gctccgtgac
    13501 gtcgtcaagc acaatcagac ggcccgtgct ttcgccctgc ttattcaaga tggtctggct
    13561 tttcatttca tagacgcgtt ttccgtttcc attgtcaaac gcaaatgctg cttgctgttc
    13621 ttcctctgaa tcggttagca gggaggaaac cggctgaatt tcggatatgt cccggctcag
    13681 catatcggcg ccctccaaac tgagcagttc gatagccgat tggttgatat caataatctt
    13741 tttatccccg tctgtaacga taacgccgct gttcatggtt tcaataagca gatcgcgggc
    13801 gatgggcatg atatttaaaa atttatgacg gtagatgctc catgccataa gcagggaggc
    13861 aggtccgaaa aatgctgggg ataaatcata tcgcgttatg ggcaacagct gaaagtaaaa
    13921 tcctacattg ggaaacacca ttagtacaat gtatagtatc agaactacgg cctgctttcg
    13981 gtacagcgaa tcctttcttc ttgccacgtt aaccagaatc gaaaaagaag cgatgattat
    14041 ggaatagata aaaagtgtgt aaagaggata gattaatcca tagtcctttt ttatcgtata
    14101 cggaatcgcc gacgtgtcca gcgaaaagtt tgtccgcata agaccgagat acggatcagt
    14161 taagacaagc gcccagaaga tggtatatac aagcgtaacg attagaacat tccgctttgt
    14221 aatatacctg tcgtacccgg caaagcgaat ggccatgagt aaaaacgcaa acgccgagaa
    14281 catcatgccg aggtattcca tgtcagccca gaacagcttt accggcagcg tcgttccaat
    14341 caactcaaac gtctggcaga acacactcca cgccgaagca agcatcacca tgataaactc
    14401 tttaacgccc acatgatgac gatactgaat tccataaatt gaaagaatga tcagtgtggc
    14461 aaaagaaaac gatgaaaaaa tgatgtatgg gaaaaattga taaatgccca ccgcctcctt
    14521 gtatcaattt atacgaatga atataaagct acattagtct tttattatat ttctaaaatt
    14581 aataattcca agtattaagt atggagcgtt attgctttaa aaagttctgt tgcatggtat
    14641 taaagctctg gtgtgagaat tttcaagtga tgatcggtaa actataaccg aatcgcgcct
    14701 ccgaaatacg tcagggagct ttctggcccc gtaacgcggc acgcatcatg tttcgcactt
    14761 ctcgccaaac gccgagctct ccgttcaggc atacgtccgg cagtctgact agggcttttt
    14821 cttttgcctt cagcaccgcg ttttcgatca tctgaaaagt gaaagcaagc tcctccggcg
    14881 tgatgttcag gatatccgct gtcaactccc cgagtggaaa ggcctgtcct tccacttcaa
    14941 tatggcttcc ctgtatgcga aaagcaattt tatcaaactt gccgtgcacc ccaattgtat
    15001 aggattgagt tttatatatt aatccgtttt tctgaatttc tccaaaatgt tccacaatta
    15061 tattgtaagg aataattatt taaaaatcaa tcccaacaac aaggacgccg atataacatc
    15121 tattgcaagc ttaaagcctt ttttagaaag cttctccaac agctcagtta tttcttataa
    15181 acacaggttt ttcactgcgc agcatgatac gcaaaaatgg gaaatggaca ggcgacatac
    15241 ttttacaacg attgcctatc catttcccga ggtttcctgc aatggaggtt acctccttat
    15301 ttaattttac cccatcgctt cagctctaat tcctgcttat ataaatcgtc attcaaaaag
    15361 gagcgggttt ctgaaaatac agttcgatca aatcgaaaca acagcggcct taaggccttt
    15421 cagtggacta cacgtaccct tcctgaaaca aatcaattcg tttcagcagg taatcaatag
    15481 cttaatcccg cggggcaccc cccttactaa ggttgtccct tttcttgggg tagcttaatt
    15541 atataactta ctgaatattc tgtcaataaa taatggagaa atattattgt ttatctttta
    15601 actatcaatt aaatgctgct gtactactcc tacgcctttc ccaagctcca caggcatgcc
    15661 tgctctgtat aatgcccttt ctacggctgc aataaccgat accatatcat gcttatcaat
    15721 atttcccata tggcccagcc tgaacattct tcctgcgtag gcagccagtc ctcctgccac
    15781 catgatgcct tcctctgcca gaagtcctct gaattttgca tcatctactc cttcaggata
    15841 aacaggattg gataatgtgg cggccctgca gccttccttt gccaatattc tgaatcccag
    15901 ggcttctaat gctgcctgca ttgctgtacc ggtttttcta tgccttgcat acctgttttc
    15961 tattccttct tcctttatga tacgcaccga ttccttcaaa gcccatatca tgtttatagc
    16021 cggtgtggca aagtacttgg ccgtatcctt cattacagga agccattttt caaaatctat
    16081 atagaactca ggaatgcttc ccaggctttt gcgcctttcc agcgctttcc cgcttgccca
    16141 gagcattgtc aggccggggg caacgccgaa ggctttctgt gagcctgtaa acaatatatc
    16201 tattcccatg gcgtcaacat cttcaggctc tcctgccgta gcgcaaacac cgtcaactat
    16261 gtaaattgtc tcagggaatt ccttcatcat cttgccgatt tccgcaaccg gcgcacaaac
    16321 ccctgtggaa gtatcaacat gagtaactgt catagccgcg tattttttct ctttcagctt
    16381 ttctttcatt ttttcaacag gtaccgtttc gccccattcg ctggagataa catctgcatt
    16441 caaacctttt ctgctgcata ggtctatgaa ccggtctcca aaaaacccgt gagatactat
    16501 cagcacattg tcgcctgcct ttgtagtatt ggctatggcc atttccatac ccatagtgcc
    16561 ggtgcctgca acaacaaata cttctccgct gcaattaaac aaagacttca aatcctccag
    16621 aagctcctta aaatccttta caaaagccgg atctccaaaa gcaacagtct ctctccccat
    16681 ctgttcctgg atggacctag ctacaggagt gggtcccgga atcatcaaca gtttcctggt
    16741 tttcatagaa atcctccttt tttactatta gtaaattatg atttacctat actttaatta
    16801 tgcgccctat cccatttaaa gtcaaggaat gaatcaccgc atgtctttcc attgcaggca
    16861 tcttatgata agctattaat gtaccctgca aatatcagtg gttaaatatg gttttaggaa
    16921 ggtgtgcccg tgtcatatct gaaacttatt gatgataaaa ttcaatcctt ggcagagtcg
    16981 ctttcatcca tacttaatgt agaaaccacc gtggtagatg agcatttgct gagaatcgca
    17041 ggaacaggag acttttacca tcgtattaat gaaagcagcc ccgaaaattc attatttgca
    17101 aaagtattga aaaccggaaa agcagacttg aacctatacg ataaaaaaaa tccgatatgt
    17161 caaacctgca acaattctga tacctgcaaa gagcttaaaa gcatgattct tccgataaaa
    17221 gttgaaaatg atgttatcgg tgtcgtaagc tttgtatcct ttaatgccgc ccaggatcag
    17281 cttatggttg aaaaaaaaga ggaatatctt gaaatcctta agcattttgc ggaaaccatc
    17341 gaaaaagaga taataagcat caaaatggtg aataagctca atatgggcat agccgaaata
    17401 aacggcatca taaactccat aaacagaagc attatcatat tgaataagga gcagcggatt
    17461 atttatataa attccaaagc aataaaatcc ctgaatatta atttttcagc cgacaagatt
    17521 attaacaaaa gaatcggcag cattattaaa ggttttaaag ctgaggatac caacaataag
    17581 gaagtcatag gcaactgggc aatagggggc aagctgataa aggtcatata caaagtaaat
    17641 tatataattc tcgataataa gaacgtctcc actataatta attttgatac tctggatgaa
    17701 ataatcagca tagccatgac ttacaacagc gatgaagaaa cgaccttcga aaatataatc
    17761 ggacgcagtc agataatgca ggacgtaatt gagaaagcaa aaatagccgc taaatccgat
    17821 tccacgatat ttctgcacgg tacaagcggt accggcaaag agttgtttgc cagatccata
    17881 cacaatgcaa gctacagaaa agacggccct tttgttgcgg taaactgtgc cgccctgcct
    17941 gaaaacctta tcgaatcgga actcttcggt tatgaaaaag gctcatttac cggtgcaagt
    18001 cagaaaggca aaacaggcaa gttcgagcag gccaataacg gcacactgtt tcttgatgaa
    18061 attgcagacc tccctctgca tcttcaggca aagctgctta gggttctcca ggataaaaag
    18121 attgacagga taggcggctc cgctcaaata aatgttaatg tcagaataat ttccgctact
    18181 cataaaaacc ttgaggaaat ggtaaggaat aacgaattca gggaagactt attctacagg
    18241 ttaaatgtaa tacccctgca cctgccgtcc ttaaaggaca gagaagaaga tgtcctcctg
    18301 tgttccgaat atataatcaa gaaattatgc cttaagatga acaaatctcc aaagcgcatt
    18361 tccaaggatg tagaaacaat gttcatgggc tacggctggc cgggaaatgt gagagagctg
    18421 gagaatgtgc tggaatatgc cattaatttt tcctttgatg atgaaatcac tgcagagaat
    18481 ttacctgaat actttctggc caactacgca aaaaaagata atgatgaata catcaatatt
    18541 gaatcaccgg aaattgataa tttaaagagc ttgggtgaca ttaccaaaga atatgaaaaa
    18601 aagcttataa gaaagcttat aagcaaatac ggcaatacta ccgagacgaa gaggaaaatc
    18661 gcagaaaagc tcggaatgag cattactact ctctatagaa aactcaacga ttattaatct
    18721 ttgcctattt atcaagcatt atattaatta tttcaatatc cgtgccttcc attccctttt
    18781 tgcctactct tccaaagctt ttgatagttt cctcaactcc gcctttaaca agaccttcac
    18841 cgcttctaaa taccttaccc cttgcgctca gctggtatcc taaaattgcc gctccgacag
    18901 cagaagatat ttttgcggca caggagggct ttgctccgtc acagaccata ccgcctatgt
    18961 ttgctattgt attgatgatt gtactggaaa tctctttata gctgcctccg ttaaggtatg
    19021 ctatccctgc accgcttccc gccgcagcac ttacagcacc gcagtatgcc gaaagattcc
    19081 ctatttgggc tttctgatga atagaaacga gattgcttat tatcaatgcc ctaaagagct
    19141 tttcttcact tgtctttaat tcccttgcat actcaattac aggaacagat accgtaatcc
    19201 cctgatttcc gctgcctgag ttaataacta ccggcagtga acaaccgctc attctggcat
    19261 cgcttccggc agcagccata gcccttgccc ttactgttac atcattgccg tagttttcca
    19321 gaagtgtttt tccgacgctg acaccatagt cattcctgat gccttcttcc gcaatagccg
    19381 tgtttactgt tacctgtttt tcaaggagtt catgcagtat atccaaatca accgtttcgg
    19441 caaattccag aatatccttt atattgagaa gagttttgtc tccggccaat ttggagtctt
    19501 tgcggccgcc gttattattc tcaaaaagaa cctttccgtc ttttacaatt ttaactatgt
    19561 tggtatgtgc atctacaata taaacctctg cggattcatt tcccgcatac accttggctt
    19621 caatataaag gttgtccact cccttcagat gataaacctt gcagtaacct tcctcggtta
    19681 atctttttac agtacagtat tgtttcctct taacactttc caaaacctgc agtcccctgt
    19741 caggatctcc ccccacgact cccgcaacag ccgcagcatc gatgcctata agccctccgg
    19801 tatttggcac cacaacacct tttacatttt ttacaatatt accgctgcac cctacctcta
    19861 tcctttccgg tgcttttccc agcaccgacc tggctttagc tgccgcataa gcaattgcaa
    19921 tgggctcggt acaaccaagg gcttcaatta attcactttt caagataaga atatagtttt
    19981 ccattacctt tttgcttaac attaattaat cccccgctct cctgtaaaac atatattaat
    20041 ttatagtaat gcaattacca tgccagattt aataattcac gccatagccc ttgtccttgc
    20101 ccgcatttat taagtttatg cctgatttgc actatgcctt gtctgcagca aaggcttatt
    20161 tttagaatat taattttcat aaatgtaaaa aatgtttcat tcatgaaaat attattcctt
    20221 ttatgacacc ttatgcatta atatgatata atatgcacaa gcgcatatta tatcatattg
    20281 cacatcagga gctgattagg attggataac tataacgaga aaaacacaaa gaccattggg
    20341 tctgttataa aagccattga aattctggaa gaactggctg aatcagaaaa aggacttgga
    20401 ttaacagaaa taagcagcag tctgaattat ggagtcagtg caacttatca tcttctcaat
    20461 acattaaagc ttcataatat aattgagcag aacaaaaaaa ccaagaaata cagaataggt
    20521 gtcggcctgt ttagaataag cagcaaggcc aaaagccaat attccctggc gaaccttgcc
    20581 atgccgtttt tggacaaact aagggcaaca gtggatgaga ccagtaacct attggtgctg
    20641 gacggatatg aagtgttata tattgctcag tccgaaagca ccaagctcct gaaaatgttt
    20701 actcagcttg gggcaaaggc tcctctgtac tgtacaggcg gcggtaaagc gattttagct
    20761 tataagcccc ttagctttca agagcttgcc ctgagtaaaa tcaactttat aaaatatact
    20821 gaaaatacca ttgtcacagc agatggtttg cgaaaagaat ttgaaaccat aaaaaaacaa
    20881 ggctattcca tagataatga ggaaagggaa gaagggatta cgtgtgcagc cgctccgata
    20941 tttgattgtt atggcgaggt tattgccgcc gtaagcatat caggcccaag ctacagaata
    21001 aaagaaaagg attcaaaaag tataatatcc agtgtggttt tgacagcaaa agaaatatca
    21061 gctaatttgg gttatatgga aaaatgaacc cctatatgca tagttcaacc ttgtttctcc
    21121 cgatggattt tgccgttaat aaagcatcat ccacccgtga aacaaactga tcaatgcttt
    21181 catcggttat gtattcccct acccctgtgc ttatcgtaat tctatcatgt tgtattccaa
    21241 agttgttttt ctctatagat ttcctgactc tttccgcaac cttatatgca tctttggagg
    21301 cctttccggg cataagtatg atgaattcat ctccgcccca tcgaaacagc ttgtccgttt
    21361 ctcttattga agctttgact atctctgtta ctacctgcaa aatccggtcc ccccaactat
    21421 gcccgtaaat atcgtttatc agcttgaagc agtcaatatc aaacatgata attgaaaaag
    21481 ggctgccgtg catctctttc atatcaacgg cagatttgac gtattcctca aatcttcgcc
    21541 tgttgtatgc acctgtcagc atgtcaaagg tcgcaagctt ctcaagctct ctgtttgcag
    21601 tctccagctc attatagtat ttttttaact cattctcatg tactttcttt tcagtgacat
    21661 cccgggtaac accgatataa cccaggaatc tatcatgctc gaaaaccggt tttacagaaa
    21721 cctcaaccca aattaaccct ttatccttac aaatgaattg aagatcatac ataatgatcc
    21781 ctcttgaatt acatgacttt ctgctatgtg cgtttttctt ccattgatcc aatacataat
    21841 tccttgactc cggaacaaga aaatccgtca ttctttttcc gatcatctca ctgctttcat
    21901 atcccgacat ttccttacac gacggactta tgtaagtaaa aatcaggttc tcatccattt
    21961 cccagattac atccttaatg ttttcaacca catatttgta gcgataatgc tgcctataat
    22021 catcaaatgt tacttctttc atacatttca cctgcaatcg aaaaaatctg ttatttttaa
    22081 ttattatatc gtataaatac ataaaaaggt acaattcatt acaaattttt gtgtttcata
    22141 tctcttcttt cgttctgttt ttgctttgtc atggttcaag ttacacgtcc tcctgtccca
    22201 tgacagcttt aaaggcttcc ggcagatacc tgataatatc accggcaatg agggaatact
    22261 cacctgcata actgctgcct atgtcaccgg ctaaaccatg tatgtatact gcagccctca
    22321 gagcgtccgt gatttcaatt ttttggccta taagtgaagc aataatcccc gtcaggacat
    22381 ctccgctgcc tgctgttgcc atgccgggat tacccgtggt attgatgtaa ctgttccccc
    22441 gggggcaggc aataacggtc cttgccccct tcagcaccag ataaacatta tatctgcatg
    22501 caaattccgc ggctttttca attatgtttt ctctcatata atcagggtcc ttatttatca
    22561 gtcttgccat ttcaccgtaa tggggagtta atatcataat ctgcctgtga ttgttcaata
    22621 tatcgggagt cagcaggttt attccttccg catcaataac cagaggtttt tgtgaatttt
    22681 cagccaggaa cttcaacagc tctccgtacc ttttcatatc agcgcatccg gagcctgtgg
    22741 caataacatc cgaaaactgt attagctccc ggttcagctc atcataattt atgttttgtt
    22801 cattaaaggc atatgttatt gcttccaatg ccatagactc aacaatacag tatatggctt
    22861 cagtcacaca tgccttgaca aggcctgaac cgctgcgcag ggcagcattg cagttcagca
    22921 ccgccgctcc tgccataccc tttgagcctg acagtattcc cactcttccg taggttccct
    22981 tgtgtgaatc cctttctctc ttttttatta tgtttcttac atcccgatgc tcagtaaggc
    23041 ttatccgtga gcctttgtcc tccgttggcg catcaaatat ctctgatatt cccaatatat
    23101 atttgtcaat gcctccgacc atttttcaca gcccctttat ttcccggcaa tcttttttat
    23161 agcagcctta cagcgtcaaa taaaagcttc agtgtaagcg ccagtgctat tgtcacaaat
    23221 atcggcttta ttacctttgc accgtttttt atggctattt tgctccccat ccttgcaccg
    23281 gctatcatgg caaggcctat gggtattcca agcatataat atatctttcc gtttatcgca
    23341 aacatcacaa gagaagtgaa attacttaca aaattcagca ccttgctgtt accggccgct
    23401 atcgtaaaat caaagccgta tacttttatg aacagaaata tcagaaaaga aaccgtcccg
    23461 ggtccgaaaa agccgtcata gaagccaagg gaaaaggcga ataaacatcc tttaataata
    23521 tttctgcctg taagttcttt gaacttatcc tcactgccga agtccttttt ggcaattgta
    23581 taaattgcag tcgcaaaaat aagcaccaat attattaatc gcagaaactt ctgatcgatt
    23641 ctcagcgcag taaatactcc tatcactgaa cctgccagag tacacggaac agtgaatttc
    23701 atcaggggaa tatgcacctt tcctgagcgt atgaatgtat atgcgcttgt aacagctcca
    23761 aaggagcttg aaaacttgtt ggttcctaat gcgaaatgag ggggtacccc tgctgccata
    23821 attgcaggaa gactgataag cccgccgcct ccggcaatag catccaccgc agctgctgca
    23881 aagcctgcga ggcacaaaaa tacaacgttt aacacagtta tttcaaattc catttgtaac
    23941 tcctgaatcc atatttataa gcccggcatc ggggcagtga gggcactgtt cctgctgctt
    24001 ttattgattt acactgctta aaaggggcgg tattacttgc ttcttgcggg atactactcc
    24061 gggcagaatt attgaattct tatcatgctt cccttcaaat gctttggata tcaattcggt
    24121 attgctgccg ataaacagca gctcggttct ttcatcggaa atgtcggtaa gcatgaacag
    24181 caccatatca agtttcttat cccggtagac cttgtccata tattcaagca tcctgtcctt
    24241 tatctcaaga agctcgctgc tgtccataga gtatacctgt cccactccca actccagctc
    24301 tcccgttttg aactgcttga aatcctagta gaagatctcc tcggcacttt tatccataag
    24361 atttgagctg gccctgaaca taatcattga aaactcatcc atatccaatc cggcaatttc
    24421 cgccaggctc tccgccgctt ccctgtccgc ctccgtacag gtgggagatt tgaatgccag
    24481 ggtatccgat aatatcgcag agcaaagaag ccctgccatc tttttgctta tttccacatt
    24541 gttctccatg tacatattcc ggacaatcgt agcagtgcag cctaagggct ggtttctgaa
    24601 gtataccggc cctacagttt caatatcacc tattctatgg tgatcgatta tctccagaat
    24661 ttccgcttct tcaaggccat ccactgcttg tgaacgctca ttgtgatcaa ccagtatcac
    24721 ctgttttctt gggcagttaa tcaatgatgc ccttgaaagc atccctatca tattgttttt
    24781 atcatccacc accgggaagt ttctgaatct tttcttgacc ataacttccc ttacgtcatc
    24841 caaataatca ttaatattaa aggtgaccaa ctcattggat ttcatgaact gccttatagg
    24901 tatgctttga tttataagcc ttgccaccgt ataggtgtca tggggcgaaa ccactatggc
    24961 acagttttgg gcctcagcca gtttctttat agaacttgct acaggtactc cccctgttac
    25021 cacgatacat tgggctccca tctcgatggc gcataactgg gcctcatatc tgttcccgac
    25081 gattacaaca tcttttgcat gtatatagct ctccatcaaa tccggattca tggcaccgat
    25141 atgcacatta ccgttatata ccgagtcttc tatatccccg accagtattt ccccgtccag
    25201 cacttcaacc atattcctgt acggagtcct ggcctgggat aatatatcat tgtcatatat
    25261 gtccataaat gaatgtgcaa tatcgtcaac tgcaattatc ccgtccagtg tcctgttgtt
    25321 atgtgaaact gcaagggtgg ttatcccgct gcttttcatt atttcccaag cctttttcaa
    25381 ggatatctcc ggtttgacaa gctccacttt cctgatattt atatccttaa cttgtgtcct
    25441 tacattatct atcagaattg gttccccggc tttgaaataa ttaagcacaa aaagtgtctc
    25501 gctgtttaat tctcctattc tcttcggaaa atatacattg cttgatgtga taatattctt
    25561 aagattggca taagctatgg atgagcatat ggaatcggta tcgggatttt tatgcccaat
    25621 tactgaaatt tcctttttca agtacattcc tccttcagcg atgctttcac cttttccgca
    25681 caatatgaga taaagctttc aaaatcctcc tgtactatac ctctggcttt atctatgata
    25741 ttgctgcatt ttgatatgtc aatttctatt ccatgcttat aattcatttg cgtattatat
    25801 cttcttgcaa gggtaaagct gttcagatcc ttcaattcca taaactctgt aaatttgcag
    25861 aagcaatccg atacggactt ttctttcaaa ccgtagtatc tgtcaaggta taatgagagt
    25921 tctttaatca ggcagtagtc cgtcaagccc ttatgaaata catccatcag ttccctttcg
    25981 gattccgcaa tattcatttc gatacccatt tctataaaat tgtgagcctt ccatagtccg
    26041 aactctgcgg gaatattaca ggcttcaatt acttccttct ctattgctgc cgccttctga
    26101 aaacagtagc ccttgtatcc gtccccatat tcctcatctc cgtagaaatc aaggcctctc
    26161 ggatttacgg tatgcgtagc catagccctt gcaaattcaa ggtactcatt tccatgcccc
    26221 ttaaggtatt tatacagccc ccatcccaca ttatgggtct ggtcacgggt aaggctgcat
    26281 tttatcacag tatcaggaaa aacagacccc aaaacagtca tattactgct gtaacccaat
    26341 actctcctgc aaaaccaaat atgtgttatc ggaaacatta gttcttcctt tctcacacgc
    26401 attaatcttc tatatctata tctgcatcta catctatatt aatatattt
//
LOCUS       DAOOAB010000035        26434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2409_length_26434_cov_16.286781, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000035 DAOOAB010000000
VERSION     DAOOAB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 26434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26434
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2409_length_26434_cov_16.286781"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1146
                     /locus_tag="PKU88_08500"
     CDS             373..1146
                     /locus_tag="PKU88_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPX69352.1"
                     /translation="MNKSGENVIQSVCRAIQIIRCFEKNEELGVTEISKMAGLHKSTA
                     FGLISTLEANKLIEKNELTGKYRLGLELFRLGTKVNSALRHIAVPYLEQLVKMYQETA
                     NFVVMDDLSIIYLEKVESTQSMRISTLVGGRLPLYCTAVGKAILANLPPEELNSKLKR
                     MEFRKYTGNTVSDRNSLMKSLELTRNRGYAEDNEELEIGLSCIAAPIYNHFNIPFAAI
                     SLSGPTFRMDEIFRKEASVTLMNYTGEISMKLGYDNNNL"
     gene            1174..2025
                     /gene="panB"
                     /locus_tag="PKU88_08505"
     CDS             1174..2025
                     /gene="panB"
                     /locus_tag="PKU88_08505"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPX69353.1"
                     /translation="MAKKKSINDFYEMKKTGEKVSWITAYDFPMASFAEQAGMDMILV
                     GDSLGMVVLGYQGTVPVTMDDCISHCQAVRRGAPNTFAIGDMPFGSYQVSDEEAVRNA
                     CRFLKEADMDAIKLEGGIRVLSRIKAISDAGIVVFGHIGLTPQSSGQLGGFKAQGRTV
                     DSARELIKDALAIQEAGARALLLEAVPPELTEYLSHKLTIPVYSIGAGLPCDGQLIIC
                     GDMLGLFQAFTPKFVKKYANVAEVIINAFKEYVADVKEEKFPEDNHVYHAIDSKEAFE
                     ALFREFE"
     gene            2395..4347
                     /locus_tag="PKU88_08510"
     CDS             2395..4347
                     /locus_tag="PKU88_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69354.1"
                     /translation="MNSTAIKILEFDKIKELLKTYTVSNLGKDLTDKLEPSLNTDTIL
                     SWMNETTEACAILQRSGSVPIHSLNGIESIVGKLGKNMTLLPEEFEVILGLLQEVGKL
                     KRFMEDKSAIAPIVSSFALSAYELEELSEEIERCIQNGAVADKASSELSRVRKKIIIL
                     EDRIKGKLESILRSPAYARYLQDGIISSRNGRYVIPVKREYRRNVDGEVMDSSVSGST
                     VFIEPSEVRKLQDELNHCRIEEEKEVCRILSMLTNLAESYKKEITTNIQVMSYYDFLF
                     AKGRFSRSLEANPVSVNTCGRLDICGARHPLIGKSAVPLDLSIGDSYRTLVITGPNTG
                     GKTVALKTIGLLTLMVQSGLHVPADPGSEFAVFTDILADIGDGQSIEQSLSTFSSHIR
                     NIISIVECAGSNTLVILDELGAGTDPGEGMGLAIAILEETYRKGAVTIATTHYSEIKH
                     YAYEKDGFENGCMEFDLDTLQPLYSLKIGEPGESNAFLIALRLGMKRDIIERAHEITY
                     KERKAYDEFMPLGRITQVKNTEMVQKHAEQTNKSIKLKEDKKNYEKISRQTSFKIGDC
                     VYISTMNRTGIVCEQEDGKGNLGVMVMQKKFIVNRKRLSLYINSKELYPEDYDFDVIL
                     ESKENRKKRKAMGKRHMEGMTIEIKE"
     gene            complement(4373..5017)
                     /gene="sigH"
                     /locus_tag="PKU88_08515"
     CDS             complement(4373..5017)
                     /gene="sigH"
                     /locus_tag="PKU88_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HPX69355.1"
                     /translation="MEVNAEEKYVERYDSMFDEEIVLSARDGNATSLEYIINKYKNFV
                     RAKARSYFLIGADKEDIVQEGMIGLYKAIRDFRNDKLSSFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYVSLNKPIYDEESDRTLLDILTATKITDPEELIISREELVSIESKIG
                     EILSELELEVLMSYLQGKSYQEIACDLDRHVKSIDNALQRVKRKLEKYLEIKDI"
     gene            complement(5091..5822)
                     /gene="rlmB"
                     /locus_tag="PKU88_08520"
     CDS             complement(5091..5822)
                     /gene="rlmB"
                     /locus_tag="PKU88_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPX69356.1"
                     /translation="MNLIEGRNPVLEALKSGREIDKIFVQKGVGEGSIRQIIAIAREK
                     KILIKEVDKAKLDGLSRTRNHQGVIASAALYKYYEVDDILQAAREKGEDPFIVILDEI
                     TDNNNLGSVIRTAEAAGVHGIIIPKRRAVGLTPAVAKISAGAIEYVPVAKVTNLNHTI
                     EYLKKEGLWIAGAEMGGEPYYGKDMTGPVALVIGSEGEGLGRLIKENCDYLVSIPMKG
                     KISSLNAAVSAAIILFEIQKQRANR"
     gene            complement(6140..6637)
                     /locus_tag="PKU88_08525"
     CDS             complement(6140..6637)
                     /locus_tag="PKU88_08525"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPX69357.1"
                     /translation="MKAIVAADMNWGIGYKGSLLVRIPEDMRFFKEMTLGKAVIMGRE
                     TFESLPGREPLKDRKNIILSRKEGYGDGRITICHSAEDIFDMLKGYKSEDVFVIGGES
                     VYKQFLPYCTEAYVTKIEHKYEADKYFPDLDSKEEWELVSVSDLHTYKDINYRFTKYK
                     NIKFL"
     gene            complement(6652..7482)
                     /gene="thyA"
                     /locus_tag="PKU88_08530"
     CDS             complement(6652..7482)
                     /gene="thyA"
                     /locus_tag="PKU88_08530"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPX69358.1"
                     /translation="MSKVDKIFIEMCKDILKNGFSTEGQNVRAKWEDGSPAHTIKKFC
                     VINRYDLAEEFPILTLRPTNLKAAIDELLWIWQKKSNNIKYLKSHIWDSWADESGSIG
                     KAYGYQLGIKHKYPEGEFDQVDRVLYDLEHNPYSRRIMTNIYNHSDLHEMHLYPCAYS
                     MTFNVTGRKLNGILNQRSQDVLVANNWNVAQYSILMHMMAQVSGLEVGEFVHVIADAH
                     IYDRHIPLVEELIQRNPYPAPKLLINKEVKDFYDFKVEDFELEGYQSGQQIKGIPVAV
                     "
     gene            complement(7579..7992)
                     /locus_tag="PKU88_08535"
     CDS             complement(7579..7992)
                     /locus_tag="PKU88_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HPX69359.1"
                     /translation="MMDSGKLSATDVKMLNPLVLAYAGDSVYDTFVRTLLVFGGHGQV
                     AKLHKMSIEFVKAKAQADILWEINELLTPEEQDIVRRGRNTKSSTMPKNADILDYRYA
                     TGFEALIGYLYLTGQIDRLMEVIRMVINVKTSRQK"
     gene            complement(8075..9397)
                     /gene="rmuC"
                     /locus_tag="PKU88_08540"
     CDS             complement(8075..9397)
                     /gene="rmuC"
                     /locus_tag="PKU88_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPX69360.1"
                     /translation="MSDIMLYIVIGLLAINSIILIIIMNTSPAGKLIKLEARLDAIEK
                     SIDNLDRTIMEGMLQNREEISGSIKIFNEMIASQMSEISNMQRNQLDIFSQQLANLTQ
                     TNEYRLDRMRDTIEEKLKYLQEDNSKKLDQMRATVDERLSSTLEQRLGESFRLVSERL
                     EMVHKGLGEMQTLASGVGDLKKVLTNVKARGTWGEIQLGNILEQILTPEQYSKNVATV
                     KGSSERVEFAVKLPGRDIKGTETVWLPIDSKFPQEDYQRLLEAQENGNPVKAEEAAKA
                     MEFKVKGFARDIRDKYISPPWTTDFGIMFLPTEGLYAEILRRPGLCDCLQREYRIVIA
                     GPTTLIALLNSLQMGFRTIAIEKRSSEVWSLLGTVKTEFGKFGDILDKTRKKLQEASN
                     TIEDAARRSRSIGRKLKNVQELASEDAEMLLEEADDEDFSQIASAKED"
     gene            complement(9422..10816)
                     /gene="cysS"
                     /locus_tag="PKU88_08545"
     CDS             complement(9422..10816)
                     /gene="cysS"
                     /locus_tag="PKU88_08545"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPX69361.1"
                     /translation="MKLYNTMTRKKEEFEPMTPGEVKMYSCGPTVYNFFHIGNARPFI
                     IFDILRRYLEYRGYKVTFVQNFTDVDDKIIKRAKDENTTEKEVSDRYIKEYFKDAEAL
                     NIRKADYHPRVTEHIKEIIDIIKRLEANSFTYTVGGDVYYDTAKFVGYGKLSKQNLEE
                     LEVGARVDVNEVKRNPFDFVLWKAQKPGEPAWESPWGMGRPGWHIECSAMSMKYLGET
                     IDIHSGGQDLVFPHHENEIAQSEGATGKPFARYWLHNAFLNINNEKMSKSLNNFFTVR
                     DIEEKFDLEVFRLFMISAHYRSPLNFSAELLDQAKSALERLYNAKNNLVYLMDKSEKK
                     PWVQDEKDFKEALLGYRNKFNEAMDDDLNTADGLAVIFDMVRDINSNVKAESSRELVK
                     VSYDMLMELTGVLGLLYKKEESLDSEIEKLIEERQKARKEKNWALADKIRDDLKEQGI
                     ILEDTPEGVKWKRV"
     gene            complement(11321..14530)
                     /locus_tag="PKU88_08550"
     CDS             complement(11321..14530)
                     /locus_tag="PKU88_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPX69362.1"
                     /translation="MNLNSDDINLYLNENIDRIYKKIIRIAEKNIGKEENFRIDIAIL
                     INDIFRDLEIENRINPEQEYSVTQGRIDSLYGNIIIEYKAPGKISSNNISINETFIEQ
                     VQRQIIGLSKRNKVDIEKILGVVFDGYNIIYVRHRNNICSVSKVIKVNKESIQLFLKR
                     LLSLSIEKKALTIENLLNDFSSDSERTRKIVSLLYKKSSNNKDYNRPKLLFEQWKHLF
                     REVCGYDFDTLDLKIQDLKQHYNISEDNVKIDYLIFSIQTYFSLIIKFLSIEVLTYLS
                     DKKDDGLNVLITDNQSKLKEQLEDIESGGIYKRLGVTNFLEGDFFGWYLSLWDDDIYF
                     EFRDLIEEFRKYDYSSINLEPDTAKDLLKDVYHSLFPKELRHNLGEYYTPDWLAEFLI
                     QEMDIEYYSSKSILDPTCGSGTFIVLLIKNYVSYNKGKIEDKKILNSIINNIKGYDLN
                     PLAVISARANYVIALGDLFNERVCEIEIPIYLCDSMLTILEQKQLDRDCHTVFTKAKE
                     FPIPVSLIDKGIMNKILDILNEAVNLDYTEDNFIQRIKNENIINEEGLNKEKAILKEL
                     YSITYNLEKEGLNGIWGNIIKNAFAPIFQKKVDYIIGNPPWIVWQSLPEEYRRSIQKY
                     WHEYRVFEHAGLDARLGSAHDDISVLMTYVIMDNFLKDKGKLGFVINQNIFQSSGGGQ
                     GFRKLMIRDSIPVKIERVHDFVKVEPFKDLGASNKTAVFTAIKNEETEFPVDYIVWDK
                     KVNGTVDSSESLENILTKIIGYKSLKAKPIKEKNSSWIIGTEEELTIYDKMVTCQDVH
                     YKARKGVDTSANCIYWVKVKKGLPNNQVLVDNSPEASRKPIKQVENTPLEEDLVYPLL
                     RGKDVQRWNAETHYNIVLPYTNDGKVIEAEELKVKYPRAYEYFYKKENGFIDILINRA
                     IYKKHYLSLNTKKIAPEYVLYNIGPYTFSPYKVVWKALASGMIATTISTNNNKLIIPD
                     HNVIMIPLYDEDEAYYISGILNAEIVSKFINAYISWFFSTHILENMYIPKFNPNNKIH
                     KKIVELSREAHDFAIQKDRKLHNTEKQVNEIVESLLLNE"
     gene            complement(14794..15111)
                     /locus_tag="PKU88_08555"
     CDS             complement(14794..15111)
                     /locus_tag="PKU88_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69363.1"
                     /translation="MFIIRNKGSGNKFTLISIIMIIAVIYIFRLISPQKEIEYPDTMH
                     YNNVKYEYAETVKGLPFMFVRKRPVSEEGFIILVRRGTDTQEEIFMYEGFRKYRRYEA
                     LRE"
     gene            complement(15187..15666)
                     /gene="ispF"
                     /locus_tag="PKU88_08560"
     CDS             complement(15187..15666)
                     /gene="ispF"
                     /locus_tag="PKU88_08560"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPX69364.1"
                     /translation="MHGFRVGNGYDVHKLAEGRKLILGGVEIPNAKGLLGHSDADVLV
                     HSIMDALLGACGESDIGRHFPDSDISYKGISSLVLLKKVKDILYSKGYRISNIDSTII
                     AEKPKLSPFVDEMKANIAAVLQLDKDCIGIKATTSEGLGFTGRGEGIAAYAAAIIYK"
     gene            complement(15676..16365)
                     /gene="ispD"
                     /locus_tag="PKU88_08565"
     CDS             complement(15676..16365)
                     /gene="ispD"
                     /locus_tag="PKU88_08565"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPX69365.1"
                     /translation="MNTAIVLAAGKGTRMNAGMNKQFMLINDKPLLAQTLAAFQSCSA
                     IDSIILVAGREELKTCKEQILDVYGFDKVDKLVSGGSERQQSVYNGILELEDDCSIVV
                     IHDGARPILPEGIIEKCIDGAKIYGAVSAGMPAKETIKILNEEGFVQYTPERGKVWVT
                     QTPQAFKRDIIEKAHKMANIKGISGTDDAFLVECMGIKVKMLEGSYENIKITTPEDII
                     LAEAIMRKTVE"
     gene            complement(16458..17579)
                     /locus_tag="PKU88_08570"
     CDS             complement(16458..17579)
                     /locus_tag="PKU88_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HPX69366.1"
                     /translation="MRKVIVWVLMILGAALGAESAYLLTNYFKVTRFLGFDLSGSGSI
                     VPVLFGGVIGGIIFYFISSWLINFGKESTTRVERMLQKIPAGEMLVGAIGLITGLVIA
                     YLISSGPIKLIPFAWLGTSLSILIYLLLGYLGVSIATKKREDILGLLIPWKRLVQKDK
                     EKQAAKVGSVMQPKILDTSVIIDGRIADICKTGFIEGPLIIPAFVLEELRHIADSSDA
                     LKRNRGRRGLDILNRIQKELDINVEIVEKDFEELTEVDTKLLKLAQYMNGKVITNDYN
                     LNKVAEFQRVPVLNINELANAVKPVVLPGEEMVVQVIKDGKEFGQGVAYLDDGTMIVV
                     DGGKRYIGDTIEVVVTSVLQTAAGRMIFVKPKSAADKVS"
     gene            complement(17733..18209)
                     /locus_tag="PKU88_08575"
     CDS             complement(17733..18209)
                     /locus_tag="PKU88_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPX69367.1"
                     /translation="MFSIGDMIYYPRHGAGIIEGIEEKEVLGEKCKYYVIRIALGDMK
                     VMVPISKTEFHGIRNITSRDRIDDIYNILSGETSCMCKSWSKRYRENEAIIRRGDVFE
                     ISELVKNLTVADRGKKLSTGEKRMLENVRQLLVSELMLMLDEPYEKIEKKVLGIIK"
     gene            18411..18806
                     /locus_tag="PKU88_08580"
     CDS             18411..18806
                     /locus_tag="PKU88_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPX69368.1"
                     /translation="MKDLLYDNFQDSVGELLVRHKSILDVLTKYQESQSRVSRAVVKA
                     VTSCGCIEINSKKQNYDNEITLQELKNYMDTHLKGSLCANCRDVIEKELGNNLFYIAA
                     LCNIMDISMYDVLLKEYEKMHTLGIYNLF"
     gene            complement(18891..19970)
                     /gene="disA"
                     /locus_tag="PKU88_08585"
     CDS             complement(18891..19970)
                     /gene="disA"
                     /locus_tag="PKU88_08585"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPX69369.1"
                     /translation="MRDEMGRDDGLQSCLRMIAPGTPLREGLESVLKAKTGGLIVVGD
                     SKEVMSLVDGGFHINSDFLPAYMYELAKMDGAIILSRDCKKILYANTQLIPDPSIPSS
                     ETGIRHRTAERVARQTGELVISISQRRNVITIYKGYLKYVLKDISIILSKANQALQTL
                     TKYKSVLDGTLANLGALEFDDIATVLDVALAVQKIEIVNRIVTEVEQYIYELGVEGKL
                     VSMQLEELVAGLKEEEVLLIRDYLPRGEENNYKEITRTLRNQSSEDLLELPFVARILG
                     LGNNINTLDLPISTRGYRILSKIPRLPMNIIENLVSDFERLQRILGASIEELDEVEGI
                     GEVRARIIKESLKRVYEQIQTERHI"
     gene            complement(19975..21339)
                     /gene="radA"
                     /locus_tag="PKU88_08590"
     CDS             complement(19975..21339)
                     /gene="radA"
                     /locus_tag="PKU88_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPX69370.1"
                     /translation="MAKIRTKFVCSECGSESPKWMGKCPECGQWNTLVEEVEQRDSQA
                     QQSFAGILSKPESLLDISEDIEERLSTGSYELDRVLGGGIVKGSLVLVGGDPGIGKST
                     LLLQIANNIGNNGKKVLYVSGEESTAQIKLRAFRMGIRSGNIFLVSENNTQNVDIYVR
                     DMKPDILVVDSIQTMYSPVITSAPGSVSQVREATLGLMKIAKQDGIATFIVGHVTKQG
                     AIAGPRVLEHMVDTVLYFEGETHMSYRVLRAVKNRYGSTNEIGIFEMGDKGLTEVPNP
                     SEMLLSGRMEGATGSCVISAMEGTRAMLVEVQALLSHTGFGVPRRNATGVDYNRVALL
                     MAVLEKRVGMQLGSSDSYVNVVGGIKLNEPAADLGIVAAIASSFRDREIDGSTVIIGE
                     VGLAGEVRAVNFAEKRVNEAAKLGFKRCIIPANNLSRLNDKSGIEVLGVSNVNNMLDI
                     IIGG"
     gene            complement(21753..24188)
                     /locus_tag="PKU88_08595"
     CDS             complement(21753..24188)
                     /locus_tag="PKU88_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPX69371.1"
                     /translation="MGLFDRFTERAQKVMVYSQEEAIRQNYNYIGTEHILLGILREGE
                     GIAAQVLKNKGVNLDTVRQQVEALVGKGQQEVGQVVGYTPRTKTILELSVEEARSLGQ
                     NYIGTEHLLLALIREGEGVAAQILAAMGLNFDSIRQEIIGLLDSEGAKGKRGEASAKG
                     SNTPNLNQYGRDLTEMAREGKLDPIIGREKEIERVIQILSRRTKNNPALIGDPGVGKT
                     AIAEGLAQKIIEGNIPEILKGKRVVTLDLSSMVAGSKYRGEFEERLKNVMNEIRESKN
                     VILFLDEMHTIIGAGGAEGAIDASNILKPALARGEIQAIGATTLDEYRKHVEKDAALE
                     RRFQPVMVGEPTKEEALLILEGLRDKYEAHHKVKITDEAIKAAVELSDRYIADRFLPD
                     KAIDLVDEAASKVRIKIFTAPPDLKKMEEELAALEKEKAEAISLQNYERAAELRDRLQ
                     HIRQKLEDGRKNWNDKASSKDSVVTEEDIASIVSGWTGIPVTKLAEEESNRLLNLESI
                     LHQRVIGQEEAVLAVSKAIRRARVGLKDPKRPVGSFIFLGPTGVGKTELSKALAEALF
                     GDENAVIRIDMSEYMEKHTVSKLIGSPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVDFKNSVVIMTSNVGVSTIRKQAVLGFATSSNE
                     REDAYEKMKENVLEELKKTFRPEFLNRVDEIIVFHQLQEEHIRKIVDLMLTSLLVRIR
                     EMNINVEVTDKAREFLAEKGYDQTYGARPLRRAIQKMVEDQLSEEMLKGEIKPGSEVL
                     VDIEDDKLIFRNK"
     gene            complement(24267..25289)
                     /locus_tag="PKU88_08600"
     CDS             complement(24267..25289)
                     /locus_tag="PKU88_08600"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPX69372.1"
                     /translation="MKGWVLGNGPQSDIILSSRIRLARNIADIPFPPGLSAEKAKEVV
                     DSVRNSLSKPESKLEFKEYIMQKLPALERQCFVERHLISPGLSRNCAGGAALISSDEM
                     VSIMVNEEDHIRIQTILPGLQIKKSWEMASDIDDILEQSLEYAYSENWGYLTSCPTNV
                     GTGMRASVMVHLPALNITGNMNKILQTVAHIGLTIRGLYGEGTEIVGNIFQISNQITL
                     GRAEEEIVENLTAVTKQIIDKEKEARRVLLESNRIKIEDKIWRSWGIMKNARVMSSQE
                     CMKLLSDVRLGVDLNIIGNIPTRLLNEIMIEAQTANIQKSSRKELTPDERDIIRAEMV
                     RSKLDS"
     gene            complement(25289..25801)
                     /locus_tag="PKU88_08605"
     CDS             complement(25289..25801)
                     /locus_tag="PKU88_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPX69373.1"
                     /translation="MICDECGKRPAAVHITKVENGKKSDMHLCEQCAMQKNLLSLSTS
                     FSVNDLLAGLLNSGGASHIKTEMVNDIKCNVCGLSYGKFRETGRFGCGNCYKVFGERL
                     NPLFKKVHGNTGHTGKIPNRAGGRIKVLREVEKLKQQLDEAIRNEEYEKAAAIRDKIR
                     DLNKAGKEEA"
     gene            complement(25827..26285)
                     /locus_tag="PKU88_08610"
     CDS             complement(25827..26285)
                     /locus_tag="PKU88_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HPX69374.1"
                     /translation="MSRISDLIEKFISDMIEESKGVVEIQRNEMANYFDCAPSQINYV
                     LMTRFTTERGYFIESRRGGGGCIKITRIDIDKDDYLMNMLSTSIGDSINKEGAFALAE
                     ALYKQKLIDCNTVNILKAATDDSTLSKVGKPYRDNLRAEILKAVIVAALV"
BASE COUNT         7080 a         6294 c         4491 g         8569 t
ORIGIN      
        1 aatatggtat tataatgcta atttatagtt ttgtcaatat ggttaaagcc tagagaaata
       61 tataactcaa gagtatatta catgcaatta atgtgacatt taatactttt tatataatgt
      121 ccgggcggga atatatgctt gcttttaatt ttgtttcatc ataaatatta tgaaactttt
      181 tataatattt attaacgcat tttgataaca catgagaaag cacaaatttc ccttgtaatg
      241 cttctctgaa tgtattacac aattgctgaa gagaggtatt gaaattaaat atatattggg
      301 ttacaataat agtgtactat attatagaat gttgttcttc attatagtac gttttggcgg
      361 ggagggatcg tattgaataa atccggtgaa aatgtaatac agtcagtatg cagagcaata
      421 caaataattc gctgttttga aaaaaatgaa gaactgggag ttaccgaaat aagcaaaatg
      481 gctgggcttc ataaaagtac tgcttttggt ttaatatcca cacttgaagc aaacaaattg
      541 attgaaaaga atgagcttac cggaaagtac agattggggc ttgaattgtt caggctgggt
      601 accaaggtta attccgccct taggcacata gccgtaccat acctggaaca gctggtaaaa
      661 atgtatcagg aaacagcaaa ctttgttgtt atggacgacc tttccattat ctaccttgaa
      721 aaagttgaga gcacacagtc aatgcgtatc agcaccttgg taggaggcag gctgcctctg
      781 tattgtactg cggtgggaaa agcgatattg gccaatcttc ccccggagga gcttaacagc
      841 aaactcaaaa ggatggaatt cagaaaatac accggcaata ccgtttctga cagaaactcg
      901 ctgatgaaat cccttgaatt aacaagaaac agagggtatg cagaggataa cgaagagctt
      961 gagatagggc taagctgcat agctgcaccc atttataacc acttcaatat tccctttgcc
     1021 gctataagtc tgtccggccc aacctttaga atggatgaga tcttccgaaa ggaagcaagt
     1081 gtgacattaa tgaattacac tggggaaata tctatgaaac tcgggtatga caataataat
     1141 ctataaaata ttaaaattta aggaggaatt caaatggcaa agaaaaaatc aatcaacgac
     1201 ttctatgaaa tgaagaagac gggggaaaaa gtctcatgga ttaccgccta cgattttccg
     1261 atggcctcat ttgctgaaca ggccggcatg gacatgatcc ttgtgggtga ctctttaggt
     1321 atggttgtat tgggatatca aggtacagtt ccggttacaa tggacgattg catatctcac
     1381 tgccaggctg tcagaagagg tgctccgaat acttttgcaa taggtgatat gccctttggt
     1441 tcatatcagg tatctgatga agaagcagta agaaacgcat gcagattcct gaaggaagcc
     1501 gatatggatg ccataaagct tgaaggcgga attcgtgtac taagcaggat taaagcaatt
     1561 tctgacgcag gaatcgttgt attcgggcat ataggcttaa cacctcaaag ctcaggacag
     1621 ttgggaggat tcaaagcaca gggaagaaca gttgacagtg caagagaatt gattaaggat
     1681 gccctcgcaa ttcaggaagc gggagcaaga gcactgcttc tggaagcagt tcctccggaa
     1741 ttgacagaat acttaagcca taaattgact atcccggtat attccattgg tgcaggactt
     1801 ccctgcgacg gccagttaat aatatgcggg gatatgctgg gccttttcca ggccttcact
     1861 ccaaaattcg tcaaaaaata tgccaacgta gctgaagtta ttattaatgc attcaaagaa
     1921 tacgtggcag atgtcaaaga ggaaaaattc cccgaagaca accatgtata ccatgcaata
     1981 gacagcaagg aagcctttga agctttattc agggaatttg agtaaataaa cattgacatc
     2041 ttgcagatat ttacatagaa taaaatatga aagtcgtaga aatcaataat ttgataaacg
     2101 ccgaaaatta aagttcgagg tattttatgt ctgagagtgt tcataactag ataaatgaat
     2161 ctagcatcga aagggaatcc tattaattgc tttgcgcatt aataggcttg gttagttgcc
     2221 cttttgatat tatggacacg gaaccaattg tatatgttgt acagtgccgt gcttttgcac
     2281 ggttttttca tatttggcgt ttatctgcag gatacgtatg actgtaccgg atataaaggt
     2341 tgtgattgga cgtagtctga ttacggcctt ttcccattta aggaggaata taaattgaat
     2401 tcaacagcta taaaaatttt agaatttgat aaaatcaagg aactgctgaa aacctataca
     2461 gtatccaatc tgggaaagga tttgacagat aagcttgaac catctctgaa tactgacacc
     2521 atattgtcgt ggatgaatga aactaccgaa gcttgtgcca tattgcagag gagcggcagc
     2581 gtaccaatcc acagccttaa cggtatagaa agtatcgttg gcaagttagg aaagaatatg
     2641 actctgcttc ccgaagaatt cgaagtaata cttggattgc ttcaggaggt tggcaagctt
     2701 aagcggttca tggaggataa atctgcaata gcccctatag taagcagctt tgccttgtcc
     2761 gcctatgagc ttgaggagtt gtccgaggaa atagaaaggt gcattcagaa cggagcggtt
     2821 gctgataagg ccagcagcga gctgtccaga gtaagaaaga aaataataat acttgaagac
     2881 aggataaagg gcaagcttga aagcattttg agatccccgg cctatgccag gtatcttcag
     2941 gacggaataa taagctcccg aaacggcaga tatgttatac ccgttaaaag agagtatcgc
     3001 aggaatgtag acggggaagt gatggacagt tcagtcagcg ggtctactgt cttcattgag
     3061 ccttcggaag taagaaagct ccaggacgaa ctgaatcact gcaggataga ggaggaaaaa
     3121 gaagtctgca ggatattgtc aatgctcaca aatcttgcag aatcatacaa aaaggaaata
     3181 acaaccaata tacaggttat gtcatattat gacttcctgt tcgccaaggg aaggttcagc
     3241 agatctctgg aagccaaccc tgtaagcgtc aatacctgcg gcaggctgga catatgcgga
     3301 gccaggcacc ccttaatagg aaagtcagcc gtgcctctgg atttaagcat cggggattcg
     3361 taccggactt tggtcataac aggccctaat acaggaggca aaactgtggc cttaaaaact
     3421 ataggactgc tgacgcttat ggtacagtcg ggacttcatg tacctgcaga tcccgggagt
     3481 gaatttgccg tctttaccga catacttgcc gatatcggag acggccaaag cattgaacaa
     3541 tccttaagca ccttctcctc ccacatcaga aatataataa gcatcgtaga gtgtgcaggc
     3601 agcaatacac tggtcatatt ggatgaactg ggggcaggca cagaccccgg tgaaggcatg
     3661 ggccttgcca ttgcaattct ggaagaaaca tataggaaag gtgcggtcac aattgccact
     3721 acccattaca gcgagataaa gcactacgca tatgaaaagg atggatttga aaacggctgc
     3781 atggaatttg accttgatac gctgcagcct ttgtatagtt tgaagatagg agaaccgggt
     3841 gaaagcaatg cttttcttat tgccttaaga ttgggaatga aacgggatat aattgaaagg
     3901 gcccatgaaa tcacatataa agaaaggaaa gcttacgatg aattcatgcc cttaggcagg
     3961 ataactcagg tcaagaacac cgaaatggtt caaaaacacg cggaacagac aaataaaagc
     4021 attaaactca aggaggataa aaagaattac gaaaaaatct cacgccaaac ctctttcaaa
     4081 attggcgatt gtgtgtatat aagcactatg aaccgcaccg gaatcgtgtg tgagcaggag
     4141 gatggaaaag ggaatctcgg agtcatggtg atgcagaaga agttcatagt aaacagaaag
     4201 aggctctccc tttatataaa ttcaaaggaa ctttacccgg aagattatga ttttgatgtg
     4261 atattggaga gcaaggaaaa cagaaagaag agaaaagcaa tggggaaaag gcatatggaa
     4321 ggcatgacaa ttgaaataaa ggaataaaaa atgcgtctca cgacgcattt ttttatatat
     4381 ctttaatttc cagatacttt tcaagctttc tcttcactcg ctgaagtgca ttgtcgatgg
     4441 atttcacatg cctgtccaaa tcacaggcaa tctcctggta ggacttccct tgaagatatg
     4501 acatcagcac ctcaagctcc aattcgctca atatctcacc aattttggat tctattgaga
     4561 caagctcctc cctgctgatt atcagctctt cggggtctgt tatttttgtt gcggtaagta
     4621 tgtcaaggag tgtcctgtct gattcttcgt cataaatggg cttattcagc gaaacatatg
     4681 aattaagcgg aatatgcttc tgcctcgtgg cagtctttat agccgttatt atctgcctgg
     4741 tgatgcaaag ctctgcaaag gctctgaagg aagacagctt atcatttcta aaatctctta
     4801 tggctttata taatcctatc atgccttcct gtactatgtc ctccttgtcg gcgcctatca
     4861 ggaagtagga tctggctttc gcccttacaa agttcttgta cttgtttata atatactcaa
     4921 gagatgtggc attcccatcc ctggcactaa gcactatttc ttcatcaaac atgctgtcgt
     4981 atctttcaac gtatttttct tctgcattta cttccaatct atcgcctcca tgtatcagac
     5041 atagaaaacc acactattat tatacatata ccttcaattt tagtcaagtt ttagcggttt
     5101 gcccgctgct tttgaatctc aaacagtata atcgcggcag aaacggctgc attaagcgat
     5161 gagatctttc ctttcatcgg gatgctgaca aggtaatcgc agttttcctt gataaggcgt
     5221 ccgagtcctt caccttcgct gccgattacc aatgcaacag ggccggtcat gtccttgccg
     5281 taatatggtt ctcctcccat ttcagccccc gcaatccaca ggccttcttt tttcagatac
     5341 tctattgtat ggttcagatt ggttactttt gcaacaggca catattcaat tgcgcctgca
     5401 gaaatctttg ccacagcagg cgtaagtcct acagctctcc tttttggtat gattatgccg
     5461 tgaactccag ccgcttcagc tgttcgaatc actgagccaa gattattgtt gtcggttatt
     5521 tcatcaagta ttacgataaa gggatcttca cccttttccc ttgcagcttg cagaatatca
     5581 tccacttcat aatacttgta caaagctgcc gaagctatca ctccctgatg gttccttgtc
     5641 cttgaaaggc cgtccagctt agctttgtcc acttccttta taagtatctt cttttctctg
     5701 gcaatggcta ttatctgcct tattgaacct tctccgacac ccttctgtac aaaaatcttg
     5761 tcgatttctc tgccggattt cagtgcctca agtacaggat ttcttccttc aatcagattg
     5821 atattgtcat catcatcttc ttcctgccgg aagtcagctt taaacatatt cttcggctta
     5881 cctgcctcat aaggtgcggc cttttcaaaa ggccttttct tttcaaaagg cttctctgtc
     5941 tttttatctt tgacagaacc cttggaatac ctgccttcat cctttacaaa cctgctgtct
     6001 ttcttttggg tttttcccgg ccttcccggc cttgaaggtt ttgaataatt cttattaaac
     6061 gccataattc tcatccttta tagttagttt tgcctcgggt atagttccgg tattcatatg
     6121 cttattatct gtaatttttt tataaaaatt taatgttttt atattttgta aacctataat
     6181 tgatatcctt gtatgtatgc aaatcactta ctgataccaa ttcccattcc tctttgctgt
     6241 ccaaatccgg gaaatatttg tctgcttcat acttgtgctc tatttttgtt acataagcct
     6301 cggtacaata gggcagaaac tgtttatata ctgattctcc gccgattaca aacacatcct
     6361 cactcttata tcccttcagc atgtcaaaaa tatcctctgc cgaatgacat atagtaattc
     6421 tgccgtcccc gtagccttcc ttcctgctta atatgatgtt ttttctgtct ttaaggggct
     6481 ccctgcccgg taatgattca aaggtttctc ttcccataat tacagctttc cccaacgtca
     6541 tctctttaaa aaacctcata tcctcaggaa tcctcaccaa taagctgcct ttatacccta
     6601 ttccccaatt catgtctgca gcaacaattg ccttcatgcc atctctcctt attacacagc
     6661 aacaggaata cctttgatct gttggccgct ctggtacccc tccaactcaa aatcttcaac
     6721 cttgaagtca taaaagtctt ttacttcttt gtttatcaac agctttggcg ccggataagg
     6781 gttcctttga ataagctcct caactagcgg gatatgcctg tcgtatatat gggcatccgc
     6841 aataacgtgc acgaactccc caacctcaag gccactaact tgtgccatca tatgcataag
     6901 aattgaatat tgtgcgacat tccaattgtt ggcaaccaga acgtcctgtg acctctggtt
     6961 aagtattccg ttaagcttcc tgcccgtaac attaaaagtc atgctgtaag cacaggggta
     7021 taagtgcatt tcatgcaaat cgctgtgatt gtatatatta gtcataatcc ttctgctgta
     7081 aggattatgc tctaaatcgt ataatacccg gtcaacctgg tcgaattctc cttcgggata
     7141 tttatgcttg atacccagtt gatatccata tgcctttcct atggatccgc tctcatccgc
     7201 ccaactatcc caaatatgac tcttaaggta tttgatattg ttggacttct tttgccatat
     7261 ccaaagcagt tcgtcaattg cagcttttag gtttgtcggc cttaatgtaa gaatcggaaa
     7321 ctcttctgca agatcatacc tattgattac gcaaaacttc ttaatcgtat gggccggact
     7381 gccatcctcc cacttggctc tcacattttg cccttctgtt gaaaaaccgt tttttaaaat
     7441 atctttacac atctcaataa atattttatc aaccttactc aatacaaaac acctcactta
     7501 cattttatcc tgcgggcttg caaaaacatc ctatttatta cattctaccc gtaaaatcca
     7561 tcccatgtcc agacaattct acttctgccg gcttgttttt acattgatta ccatccttat
     7621 tacttccatt aggcggtcaa tctgtccggt cagatacaag tatcctatca gggcttcaaa
     7681 accggtagca tacctgtagt caagaatgtc tgcgtttttc ggcatggttg acgactttgt
     7741 atttcttccc ctccgtacaa tgtcctgctc ttcgggagtc aacagctcat ttatctccca
     7801 cagaatatcc gcctgggctt ttgccttcac aaactcaatc gacattttgt gaagcttcgc
     7861 aacctgcccg tggcctccga ataccagcag ggtcctgacg aaagtgtcat agactgaatc
     7921 ccccgcatat gcaagcacga gagggttcag catcttgaca tccgtagccg aaagcttgcc
     7981 gctgtccatc ataagctgat gcagctcctc cagcagtttt ttgtagttct tttccaaata
     8041 atcatttccc ataatatacc tactattcca atctctaatc ctcttttgcg gaagctatct
     8101 gggagaagtc ctcgtcatcg gcttcttcca gcagcatctc agcatcctcc gatgccagct
     8161 cctgcacatt cttcagcttt ctgcctatgc ttctggatct tcttgcagca tcttcaattg
     8221 tgttgcttgc ctcctgaagc tttttccgtg ttttgtccag tatgtcaccg aactttccga
     8281 attctgtctt gaccgtccca aggaggctcc atacctcgct ggaacgcttt tctatcgcaa
     8341 tagtcctgaa gcccatctgc aggctgttta ataatgcaat caatgttgtg ggcccggcta
     8401 taactatcct gtattcccgc tgcagacagt cgcacagccc cggccttctt aaaatctcgg
     8461 cataaaggcc ttccgtaggt aaaaacatta ttccaaagtc agttgtccag ggggggctta
     8521 tgtatttatc ccttatatcc ctggcaaagc cttttacctt aaactccata gcctttgccg
     8581 cttcttccgc tttaacggga ttcccattct cctgcgcctc cagcagcctc tgataatcct
     8641 cctgagggaa cttggaatca ataggcagcc ataccgtttc tgtccccttt atatctctcc
     8701 ccggcagctt tactgcaaat tcaacccttt cagaggaacc ctttactgta gccacgttct
     8761 tcgagtactg ctccggtgtc aaaatctgct ccagtatgtt gccaagctgg atttctcccc
     8821 aggtccctct ggccttgaca ttggtaagca ccttttttag gtcccccact cccgaggcca
     8881 gtgtctgcat ttctcccagt cccttgtgaa ccatttccag cctttcgctt accaacctga
     8941 aggattcccc aagccgctgc tcaagggtgg agcttagcct ttcgtccaca gttgccctca
     9001 tctggtcaag ctttttactg ttatcctcct gcagatactt cagcttttcc tctattgtat
     9061 ccctcattct gtcaagcctg tattcgttgg tctgcgtaag atttgcaagc tgttgggaaa
     9121 atatgtccag ctggttgcgc tgcatattgg atatttccga catttgagag gctatcattt
     9181 cgttaaatat ctttatcgag cctgaaatct cctctctgtt ctgcagcatc ccctccataa
     9241 tcgtcctgtc cagattgtca atgctcttct caatggcatc aagcctggct tcaagcttta
     9301 tcagcttacc agccgggctg gtattcatta tgataataag aataattgaa tttattgcaa
     9361 gcaagcctat gacaatataa agcattatat cagacatggt tatacttcct ttatttaaat
     9421 tttataccct tttccacttg actccctcgg gggtatcctc aagaattatt ccctgttctt
     9481 tcagatcatc tcttattttg tcggccaatg cccagttctt ttcctttctt gccttctgcc
     9541 tttcctctat aagcttttcg atttccgaat ccagactttc ttcctttttg taaagcaagc
     9601 ccagtactcc cgtaagctcc ataagcatgt cataggatac tttcaccagt tccctggagg
     9661 attctgcctt gacgttggaa tttatatctc ttaccatgtc aaaaataaca gccaatccgt
     9721 cagcggtatt caagtcatca tccatagcct cgttgaactt gttcctgtac cccaaaagag
     9781 cctctttgaa atccttctca tcctgaaccc aaggcttctt ctctgactta tccataagat
     9841 aaacaaggtt gttctttgca ttatacagcc tttccagtgc actttttgcc tgatccaaaa
     9901 gctcggcgct gaaattcaaa ggactcctgt aatgagccga aatcataaaa agcctgaaga
     9961 cctcaaggtc aaatttttct tcaatatccc ttactgtgaa aaagttgttc agggatttgg
    10021 acatcttttc attatttata ttgaggaacg cattatgcag ccagtacctg gcaaaaggct
    10081 ttcccgtcgc gccttcactc tgtgcgattt cattttcatg atggggaaat accaggtcct
    10141 gtcctcctga atggatatct atagtctctc ccaggtattt catagacatg gcggagcatt
    10201 caatgtgcca tcccggcctg cccattcccc aggggctttc ccatgcaggc tctccgggct
    10261 tttgagcctt ccaaagcaca aaatcaaagg gattcctttt tacctcattt acatctaccc
    10321 tggcgcctac ctccagctct tcgagattct gcttggaaag cttgccgtat ccgacaaact
    10381 tcgcagtatc ataatatacg tctccgccga cagtgtatgt aaagctattg gcttccagcc
    10441 tttttattat gtctattatt tccttgatat gctcggtaac cctgggatga taatcagcct
    10501 tcctaatatt taaggcttca gcatccttga agtattcctt tatgtacctg tcagatacct
    10561 ctttctctgt tgtgttctca tccttggctc ttttgattat tttatcgtct acatccgtaa
    10621 aattttgaac aaaggttacc ttataccctc tatattcaag atatctcctt agaatatcga
    10681 atattatgaa tggccttgca tttcctatat gaaagaagtt gtagacagtc ggcccgcagg
    10741 agtacatttt tacttccccc ggcgtcatcg gttcaaactc ttcctttttc cttgtcattg
    10801 tgttgtacag cttcattttt aacaccttcc tatacataaa tattaagctt gaggcatata
    10861 tcaaaataga taaacccgcc tcttaaagag gcgggcaatt ccgcggttcc actcttttga
    10921 tactctcaag aattaacggt aatccgccgg agaataccac tttacaagca ttcagcttcc
    10981 tctccggtgc tcacgggtgc acttcaatca gcgtatcatg aaacctctct caaccgacga
    11041 aggttcctct ctgtatgcct ttctgattta ctcttcccga tccgcgcatc atttattcaa
    11101 ttgtaatttc accttcaatt atatatatta ttatagttaa tagatattat gtcaatactt
    11161 tttataacaa tattccaatt gtagcctgta aagttgtatg ccttactatt ctctcctatc
    11221 gtccggctcg ataggctcac cctggtattt tgacggactg taatctgaca ccatatttac
    11281 tcaaccatac ttgcttttga ttgggcatag ccctagcaga tcactcattt aataacaagg
    11341 attcaacaat ttcattaact tgtttttcag tattatgaag cttcctatcc ttttgtatgg
    11401 caaaatcatg tgcttcccta gataactcaa ctattttctt gtgaattttg ttattcggat
    11461 taaacttagg tatatacata ttctcaagaa tatgagttga aaaaaaccat gaaatatatg
    11521 cattaataaa tttgctcact atctcagcat ttaatatccc agatatataa taagcttcat
    11581 cttcatcata taaaggtatc attatgacat tgtgatcagg aataattaat ttattattat
    11641 ttgtagaaat tgtggtggca atcataccag aagccaaagc cttccaaact actttataag
    11701 gtgaaaatgt atatgggcct atattatata atacatattc cggggctatt ttttttgtat
    11761 tcagggataa ataatgtttc ttataaatag ctctatttat taaaatatca ataaatccat
    11821 tttccttttt ataaaaatat tcatatgctc ttggatattt aacctttagc tcttctgcct
    11881 caatcacttt accatcattt gtataaggaa gaactatatt gtaatgtgtt tcagcattcc
    11941 atctttgaac atcttttccc cttagtaatg gataaactaa atcttcctcc aaaggtgtat
    12001 tttcaacttg tttaataggt tttctgctgg cttccggact attatctact aaaacttgat
    12061 tgtttggcaa accttttttt acttttaccc aataaataca atttgcgctg gtatctacac
    12121 cttttcttgc tttgtagtgc acatcttgac aagttaccat tttatcatat atagttaatt
    12181 cctcttcagt tccgataatc caagaagagt ttttttcttt aataggctta gcctttaagc
    12241 ttttatatcc tattattttt gtcaaaatat tttctaatga ttcgcttgag tctaccgtac
    12301 catttacttt tttatcccat acaatataat caactggaaa ctctgtttct tcatttttta
    12361 ttgcagtaaa tactgccgtt ttattacttg cacctaaatc tttaaagggc tcaactttta
    12421 caaaatcatg aactctttct atttttactg gtattgagtc cctaatcatt aactttctaa
    12481 aaccttgccc tcctccgctt gattgaaata tattctgatt tattacaaaa cccaatttac
    12541 ctttgtcttt taaaaaatta tccataataa catatgtcat taaaactgat atatcatcat
    12601 gtgcacttcc caatctagca tctagtccgg catgctcaaa taccctatat tcatgccaat
    12661 atttttgaat agaccttctg tattcttccg gaagactttg ccatactatc caaggaggat
    12721 tcccaataat ataatctact tttttttgaa aaataggtgc aaatgcattt ttaataatat
    12781 tgccccatat gccatttaaa ccttctttct ccaggttata cgttatagaa tacaattctt
    12841 ttaagatagc tttctctttg tttagcccct cttcatttat gatattttca ttttttattc
    12901 gttgtataaa attatcttct gtataatcta aattaacagc ttcgtttaaa atatctaata
    12961 ttttattcat tatacctttg tctattagac ttaccggaat tggaaattct tttgcttttg
    13021 taaatacagt atgacaatct ctatctaatt gtttttgttc taatatagtt aacatagaat
    13081 cacaaagata tataggtatt tctatttcac aaaccctttc attaaataaa tcacctaatg
    13141 caattacata attggctctt gctgaaataa cagctaatgg atttaaatca tagcctttaa
    13201 tattatttat aatactgtta agaatttttt tatcttctat ttttccttta ttatagctta
    13261 cataattttt aattaataaa acaataaaag taccagagcc acaagttgga tcaagtatgc
    13321 ttttgcttga ataatattca atatccatct cctgaatcaa aaattcagcc agccaatctg
    13381 gtgtataata ctctcctaaa ttatgtctta attctttagg gaataagctg tgatagacat
    13441 ctttaagcaa atccttagcg gtatctggtt caaggttaat actactataa tcatatttcc
    13501 taaattcttc gattaaatct ctaaattcaa aataaatatc atcatcccat agagataaat
    13561 accagccaaa gaaatcccct tctaaaaaat tagtaactcc taatctttta tatattccac
    13621 ctgattctat atcctcaagc tgttctttta attttgattg gttatctgtt attaatacat
    13681 ttaacccatc atctttctta tcagataaat atgttaaaac ttcaatagat aaaaacttaa
    13741 taattaacga aaaataggtc tgtatagaga atattaaata gtctatttta acattatctt
    13801 cactaatatt ataatgttgc tttaagtctt gaatttttaa gtccaatgta tcaaagtcat
    13861 aaccacaaac ttctctaaat aaatgcttcc attgctcgaa taatagctta ggcctattat
    13921 aatctttatt attggaactc tttttataaa gcaaagatac aatttttctt gttctttcgc
    13981 tatccgaact aaaatcattt agcagatttt ctatcgttaa ggcttttttc tcaatactta
    14041 aagataataa tcttttcaaa aataattgta ttgattcttt attaactttt ataacttttg
    14101 atacgctaca aatattattc ctgtgtctta catatataat attatatcca tcaaaaacaa
    14161 cacctaagat tttttctata tccactttat ttcttttaga taaacctatt atttgccttt
    14221 gtacctgctc aataaaagtt tcattaattg aaatattatt acttgatatt ttccctggag
    14281 ctttgtattc tataatgata ttaccatata gggaatctat tcttccttgt gtaacactat
    14341 attcttgttc cggattaatt ctattttcaa tttctaaatc cctaaatata tcattaatta
    14401 atattgctat atctattctg aaattttctt ctttacctat attcttttct gctattcgta
    14461 taattttttt atatatccta tcaatattct catttaaata taagttaata tcatcactgt
    14521 taagattcat tttatcaccc ttacatattt tatttattag ttctcctata ttcaatcaga
    14581 tatgaaatat ttgatttctg tgactttttt attaatatgc tatgactcct cttttatact
    14641 taccaagtca ttaatttgaa tctcccagct tattgctatt tttctcaatt ataacatgat
    14701 cattagcaca aaacaagaac atatgttcga ttttatgaaa atcttttcgt aatttccttt
    14761 tttaggatgt cgcttcttgg ttattcttat cctctattct cttaacgcct catagcgcct
    14821 gtatttacga aatccttcgt acataaagat ctcttcctga gtgtcggtac ctcttcggac
    14881 aagtattata aatccttcct cgcttaccgg cctcttcctc acgaacataa acggaagacc
    14941 cttcacagtc tccgcatact catattttac attattataa tgcattgtat cgggatattc
    15001 tatctccttc tggggcgaaa taagcctaaa aatatatatg actgcaatta tcattataat
    15061 cgaaataagc gtgaatttat ttccgctgcc tttatttctt attataaaca ttttcaggcg
    15121 cctcccttcg tataaaatgg ctggaagcga gcaagacaac tcagtattgc gacgcttacg
    15181 gacattttat ttatatatga ttgctgcggc ataagccgca attccttcac ctctgccggt
    15241 aaaacccaaa ccttccgaag tggtggcctt gatacctatg caatccttgt caagctgcag
    15301 cactgctgct atatttgcct tcatttcatc tacaaacggc gaaagttttg gtttttcagc
    15361 tattattgta ctgtcaatat ttgaaatccg atatcccttg gaataaagaa tatccttgac
    15421 ttttttcaat agaacaaggc ttgatatccc tttataggat atatcactgt caggaaagtg
    15481 cctgcctata tccgattccc cgcaggcacc caataatgca tccattattg aatgcacaag
    15541 cacatctgcg tccgaatgcc ccaacaggcc tttcgcatta ggaatctcta cgcctcccag
    15601 tatcagcttc cttccctctg ccagcttatg tacgtcgtag ccgtttccaa ccctgaatcc
    15661 atgcatgctt tcacctcatt ccacagtttt tctcattatc gcttcagcaa gaattatgtc
    15721 ctcaggagtt gtaatcttga tattctcata gcttccctcc agcattttta cctttatccc
    15781 catacactca accagaaacg catcgtcagt gcctgatatg cctttgatat tggccatttt
    15841 gtgggctttc tcaattatat ccctcttaaa agcctgaggc gtctgggtaa cccatacctt
    15901 tccccgctca ggcgtatatt gcacaaaacc ctcttcattc aatatcttta tagtttcttt
    15961 tgccggcata ccggcggaaa ccgctccgta aatttttgca ccgtcaatgc acttttcaat
    16021 aattccctcc ggcaatatag gcctggctcc gtcatgaatt acaactatgc tgcaatcatc
    16081 ttccagttcc agtataccat tgtaaaccga ctgctggcgc tcactgcccc ctgagaccag
    16141 cttgtccact ttatcgaagc catagacatc aagtatttgc tccttgcagg tcttaagctc
    16201 ctccctgcct gcaacaagta tgatgctgtc tattgcactg cagctttgaa aagccgccag
    16261 agtctgtgca agaaggggct tatcatttat cagcataaac tgcttgttca ttcctgcatt
    16321 cattctggtc cctttgcctg cagcaagtac aattgcagta ttcatagccc ttcaatcccc
    16381 cattttttaa taaatatacc ctaagcggac tttatttctg cttagggtaa aacaaatgcg
    16441 gcttgcgccg caatgtttta cgatacttta tctgcggcgg attttggctt tacaaatatc
    16501 atccttccgg ctgcagtttg cagaacgctt gtcactacaa cctcaatagt gtctccaatg
    16561 taccgttttc cgccatctac aactatcatt gtcccgtcat caagatatgc gactccttgg
    16621 ccaaactcct tgccgtcttt tattacctgc actaccattt cttctcccgg aagtacaaca
    16681 ggcttcaccg cgtttgcaag ctcattgata ttcaaaacag gcaccctttg gaattccgcc
    16741 actttattca gattgtaatc gtttgttatt accttgccgt tcatatactg agccagcttc
    16801 aaaagcttgg tatcaacctc ggtcagttct tcaaaatcct tttccactat ttctacgttt
    16861 atatccagtt ccttttgaat cctgtttagt atatccaaac ctctgcggcc cctgtttctt
    16921 tttaatgcat ccgatgaatc cgcaatatgt ctgagttcct ccaggacaaa tgcaggtatg
    16981 ataagcgggc cttcaataaa ccctgtctta catatatccg ctattcttcc atctatgata
    17041 acacttgtat cgagaatttt gggctgcata acgcttccaa cctttgcagc ctgcttttcc
    17101 ttgtccttct gcactaatct tttccaagga ataagcaatc ctaatatgtc ctcccttttt
    17161 ttagttgcta tacttactcc caggtatccc agaagcaaat atatcagtat tgacaacgaa
    17221 gtgcccaacc atgcaaaagg tataagcttt ataggaccgc tgcttataag ataagcaata
    17281 acaagccctg taatcagccc tatcgctccc accagcattt cgccggctgg tattttttga
    17341 agcattctct caactcttgt ggtagactcc ttaccaaaat taatcagcca ggaagatata
    17401 aaataaaata taattccacc aataaccccg ccgaataaaa ccggcacaat agaacctgaa
    17461 cctgaaaggt caaaaccgag aaaccgtgtc actttaaaat aattcgtaag aagatatgcc
    17521 gactccgcac ccagagcggc acccagaatc attaacaccc aaacaataac ttttcgcatt
    17581 tacaacttca cctcctttta tctagtatat ccagtttgat actttttaag ctataataaa
    17641 ccaattataa gaccaattat attataacaa ataaatctgc attaaaaaat aacaattgcc
    17701 tatttattta aaccaaatat ataaataatt tactatttta taatgcccag cacctttttc
    17761 tctattttct catagggctc atccaacata agcataagct cgcttaccag cagctgcctt
    17821 acgttttcaa gcatcctttt ctcccctgtg gacaattttt tcccgcgatc tgcaaccgta
    17881 aggtttttta caagttcaga tatctcgaaa acatcgcccc tccttattat tgcctcattc
    17941 tccctgtacc tcttgctcca gctcttgcac atacagcttg tttctcccga cagtatatta
    18001 tatatatcgt caattctgtc cctggatgtt atatttctga tgccatgaaa ttccgtcttg
    18061 cttatgggca ccatcacctt catatctccc aatgcaattc ttataacata atacttgcat
    18121 ttttcaccaa gaacttcttt ttcctcaata ccttcaatta tccctgcccc atgacgggga
    18181 taatatatca tatcacctat actaaacatt gatttgcctc cagtcgattt ttacaatctt
    18241 acttgatttc cataatcttc tatagccgat agaatgtcca cattgcaatt ataaattgac
    18301 attggaattc cttatactta taatggatat ataacaaagc tgaaacagca gctgacaata
    18361 tcagtttgat actatatttt atgtttactt ttatatagga gtgatacctg atgaaagatt
    18421 tgctttatga taacttccag gacagcgttg gtgaactctt ggtacgccac aaaagtattc
    18481 ttgatgtgct gacgaaatat caggagtcgc agtcgagagt gagcagagct gtggttaagg
    18541 cggtaacctc ctgcggatgt atagaaatca actcgaaaaa acagaactac gataatgaga
    18601 taacgctgca ggagctgaag aattatatgg atacccatct taagggaagc ctttgtgcga
    18661 attgcagaga tgttattgaa aaagagctgg gcaataatct tttctatatt gcagctctat
    18721 gcaacataat ggatatcagc atgtatgacg tacttctgaa ggaatatgaa aaaatgcata
    18781 cccttggaat ttacaatttg ttttaaataa ataaatgaga cagggggacg tttctcttgt
    18841 cccgcaaacg ggacaagaga aacgtccccc tgtctcatcc ccctgtctca ttatatatgc
    18901 ctttcggtct gtatctgctc atatactctc ttgaggcttt ccttgattat cctggccctg
    18961 acctctccta tgccctccac ttcatccagc tcctcgatgg aagccccaag aatcctttgc
    19021 agcctttcaa aatcagatac caggttttca attatgttca tgggcagcct tggaatttta
    19081 ctcagtatcc tatatcctct tgttgatatc ggaaggtcta aagtattgat attattcccc
    19141 aatcccaata tcctggctac aaaaggcaac tcaagaaggt cctctgaaga ctggttcctc
    19201 aaggttctgg ttatttcctt ataattattt tcctctcccc tgggcagata gtccctgatc
    19261 aggagtactt cctcctcctt caggcctgca acaagttctt ccagctgcat actgacaagc
    19321 tttccttcaa cgcccagttc atatatgtat tgttcaacct cagtcacaat cctgtttaca
    19381 atctcaattt tctgtactgc cagcgctaca tccagcacag ttgcaatatc atcaaactct
    19441 aaagccccaa gattcgccaa ggttccgtcc agcaccgatt tatacttggt cagggtctgc
    19501 agcgcctgat tggctttgga cagtattata cttatatcct taagaacata tttcagatat
    19561 cctttataaa tagttatgac gttccttctt tgagaaatag aaataaccaa ttccccggtc
    19621 tgccgtgcaa ccctctccgc tgttctgtgc ctgataccgg tttcggaaga tggtattgaa
    19681 ggatcgggaa tcaattgagt attggcatat agtattttct tgcaatccct gctgagtatg
    19741 attgcaccgt ccatctttgc caactcgtac atgtacgccg gcaagaagtc cgaattgata
    19801 tgaaagcctc catctacaag ggacataacc tccttactgt caccaaccac aatgagtccg
    19861 cctgttttcg ccttcagtac gctttccagg ccttccctca gaggagtgcc gggagcaatc
    19921 atcctgaggc agctctgaag gccgtcatcc cttcccattt catctctcat atgcctatcc
    19981 tcctattatt atgtcaagca tattatttac atttgacacc ccaagtacct ctatgcctga
    20041 tttatcatta agcctgctta aattgttcgc gggtattatg catcttttaa aaccaagctt
    20101 cgcagcttcg tttaccctct tctcggcaaa gttcaccgct cttacttctc cggcaagccc
    20161 tacttctcca ataattacag tgctgccgtc aatttccctg tctctgaagc tggaggcaat
    20221 agctgcaact attccaaggt ctgctgcagg ctcgttaagc ttgatccctc ctactacatt
    20281 cacatatgaa tcactgctcc caagctgcat accgactctc ttctccagta cggccatcag
    20341 cagcgcaacc ctgttgtaat caactcccgt agcattcctt cttggaaccc cgaaacctgt
    20401 atggctgagt aaagcctgaa cctctaccag cattgccctt gtaccttcca tagcagaaat
    20461 aacacaggat ccggtggctc cctccattct tccggacaaa agcatctcag aaggattggg
    20521 cacctctgta agccctttat ctcccatttc aaatattcct atctcatttg tggagccgta
    20581 tctgttttta acagccctaa gcactctgta tgacatatgg gtctctcctt caaaataaag
    20641 aacagtgtca accatatgtt caagcaccct ggggcctgct attgctccct gctttgtcac
    20701 atgccccaca ataaaagtcg ctataccgtc ctgttttgca attttcataa gcccgagggt
    20761 cgcctccctg acttggctga cactgccggg agccgatgtt attacagggc tgtacattgt
    20821 ttgtatagaa tccaccacca gaatatcagg cttcatgtcc ctgacataaa tatcaacatt
    20881 ctgagtgttg ttttcggata ccagaaatat attccccgat ctgatgccca ttcggaatgc
    20941 cctcagcttt atctgcgcgg ttgattcttc tccggataca tataagactt ttttgccgtt
    21001 gtttcctata ttgtttgcaa tctgaagcag caaggttgac ttcccgattc ccggatctcc
    21061 tcctacgagt accagggaac ctttgacaat gccccctccc agaaccctgt ccaattcata
    21121 gctgcctgtg cttagtcttt cctcaatatc ctccgaaata tccaacaggg actccggctt
    21181 cgacagaatt cctgcaaaac tttgctgagc ctgggaatcc ctctgttcca cttcttcaac
    21241 aagagtattc cactgtccgc actcggggca tttgcccatc catttgggtg attcgcttcc
    21301 gcattcgctg catacaaatt ttgttcttat tttagccatt taaacacctc aagaattata
    21361 tcggaatttt cacagatttt gtaaatcaat atgagtattt cccaatcatt ccaacttttt
    21421 tcactgaaac atattcttat tatactatat ttgacaatag aagaataatg cgtcgcattt
    21481 ttcgggcacg aggtacgagg cgcgaggttt aggtcagagc ttcgaagcca ttaatgccgg
    21541 gtaacacggg agacacggct gtttcacgga attcacggaa aactatatat ttccccgccg
    21601 tgaatttagt ggctttcagt gttttcagtg ttatgcgtta tttacccgta aggaataccc
    21661 aagcataccc cgcaaccccg taacctcgag gcctcgcaac ctgaagaaaa ttgctgcgca
    21721 attttcttct tttgcgatac cccccggata ttttatttat tcctgaaaat cagcttgtca
    21781 tcctcaatat ccaccaacac ttccgaaccc ggctttattt cccccttaag catttcctcc
    21841 gatagctgat cctctaccat cttttgtatg gcacggcgca gaggccttgc cccataagtc
    21901 tggtcatagc ccttttctgc cagaaattct cttgccttgt cggtaacttc cacattgata
    21961 ttcatttccc tgattctgac aagaagcgac gtaagcatca aatctacaat ctttcggata
    22021 tgttcttcct gcagctggtg gaatacgata atttcatcga ccctgttcag gaactccggc
    22081 ctgaaggttt ttttaagctc ttccaggaca ttttctttca ttttttcata ggcatcttct
    22141 ctctcgttag atgatgtggc aaacccaaga acagcctgct ttcttattgt acttacccca
    22201 acattggagg tcataataac aactgagttt ttgaagtcaa ccgttcttcc cttgccgtca
    22261 gtcagcctgc cgtcatcgag tatctgaagc agtacattga atacatccgg gtgagccttt
    22321 tctatctcat caagaagtat gaccgaatat ggctttcttc ttaccttttc agtcagctgc
    22381 cccccttcct catagcccac atagcccgga ggggaaccta taagctttga tactgtatgc
    22441 ttttccatgt attcggacat atcaatcctg attactgcat tttcatcacc gaacaatgct
    22501 tctgccagag cctttgaaag ctcggttttc cctactcctg tggggcccag gaatataaag
    22561 gaacccaccg gccttttagg atccttcagc cctactcttg cccttctgat tgccttggaa
    22621 actgccaata ccgcctcttc ctgcccgata acccgctgat gcaatattga ttccagatta
    22681 agcaatctat tggattcctc ctctgccagc ttggttacag gaatcccggt ccagccggat
    22741 acgatggaag caatatcttc ttcggtaaca acggaatcct ttgagctggc cttgtcattc
    22801 cagttcttcc tgccatcctc aagcttttgc ctgatatgct gcaatctgtc ccgaagctct
    22861 gccgccctct cataattctg aaggcttatt gcttcagcct tttccttttc caaagctgca
    22921 agctcctcct ccattttttt aagatccgga ggcgcggtaa atatctttat tctgactttg
    22981 gacgcagctt catccaccag gtctatggcc ttatcgggaa ggaacctgtc tgcaatatac
    23041 ctgtcggaca attcgactgc cgcctttatt gcttcatcgg ttatcttaac cttatgatgg
    23101 gcttcatatt tatctcttaa gccttcaagt ataagcaaag cctcctcttt tgtgggttcc
    23161 cccaccatca caggctgaaa acgcctttcc agtgccgcat ccttctcaac atgcttgcgg
    23221 tactcatcaa gagttgtcgc cccgattgcc tgaatttcac ctcttgccaa agcaggtttc
    23281 aatatattgg aagcgtctat agccccttca gctccgcctg ctccgataat ggtatgcatt
    23341 tcatccagaa aaagtatcac attctttgat tcacgtattt cattcattac gttcttgagt
    23401 ctttcctcaa attctcccct gtacttggag ccggccacca tggaagacaa atccaatgta
    23461 acgacccttt tcccttttag tatttcggga atattccctt caattatttt ctgggccaat
    23521 ccttctgcaa tggctgtttt gcctaccccg ggatcgccga tcagggccgg attgtttttt
    23581 gtccttctgc tcaatatctg tattacacgc tctatttctt tttctcttcc tataataggg
    23641 tccagcttcc cctccctggc catttcagtc agatccctgc cgtattgatt cagattcggt
    23701 gtgttggagc cttttgccga tgcctcgccg cgcttgccct ttgccccttc gctgtccagc
    23761 agtcctatta tctcctgcct tatactgtcg aaattcaggc ccattgccgc aagtatctgt
    23821 gccgccacac cttcaccttc gcggattaac gccaaaagca gatgttcagt tccgatatag
    23881 ttttgcccca agcttcttgc ctcttctaca ctcagttcaa gaatcgtctt tgttctcgga
    23941 gtatagccta caacctgtcc aacttcctgc tgcccctttc cgactaaggc ctccacctgc
    24001 tgtctcacag tatccaaatt aactccctta tttttaagca cttgggcggc aatgccctct
    24061 ccttccctta ggatacccag caatatgtgt tcagtgccta tatagttgta attctgccgg
    24121 atagcttcct cctgtgaata taccattacc ttttgggctc tctcagtaaa tctatcaaat
    24181 aatcccatat tatttcctcc tatatgtctt gctgcgggct tcgcagctta gctcttccgt
    24241 tgaagccctg tttgaaattc ccgtatctaa ctatcaagtt tgcttcttac catctctgct
    24301 cttattatat ctctctcatc cggagttaat tctttcctgc tgctcttttg tatatttgcc
    24361 gtttgggcct ctatcatgat ttcattcaaa agccttgtgg gaatattgcc gataatattc
    24421 aaatccaccc ccagccttac atccgaaagc agcttcatac actcctgcga actcatcact
    24481 ctggcatttt tcataatacc ccaggaacgc caaattttat cctctatctt tatcctgttg
    24541 ctttccagca gtacccttct tgcttccttt tccttgtcaa ttatttgctt tgttacggca
    24601 gtgagatttt ctactatttc ctcttccgct cttccaaggg ttatttggtt ggaaatctga
    24661 aatatattac ccacaatttc tgtgccttcg ccatacaaac ctcttattgt aaggcctatg
    24721 tgagctactg tctgaagtat cttgttcata ttgcccgtta tattgagtgc cggcagatgc
    24781 accattacag atgctctcat acctgttccc acgttggtag ggcagcttgt aagatagccc
    24841 cagttttcac tgtatgcgta ttcaaggctc tgctccaaaa tatcatctat atcactggcc
    24901 atttcccagg atttctttat ttgaagcccc ggaagtattg tctgtatcct tatatgatcc
    24961 tcctcattga ccattatgct taccatttcg tcgctgctta taagcgccgc ccctccggcg
    25021 cagttcctgg aaagccccgg acttataagg tgcctttcca caaaacactg tctctccaag
    25081 gccggaagct tctgcatgat atattccttg aattccaatt ttgattccgg cttggaaaga
    25141 ctgtttctga cgctatccac aacttctttt gctttttcag cacttaatcc tggcggaaac
    25201 ggaatatccg ctatgtttct ggccaatctt atacggctgc tcaaaattat gtcgctctga
    25261 gggccgttgc ccagcaccca gcctttcatc atgcttcctc ctttcctgcc ttgtttaaat
    25321 ccctaatctt atcccttata gctgcggctt tttcatactc ttcatttcgt attgcctcat
    25381 ccaactgctg tttgagtttt tcgacctccc gcagcacctt tatcctgccg cctgccctgt
    25441 ttggtatctt gcctgtatgg ccggtattgc catgaacctt tttaaaaagc ggattaagcc
    25501 tttcaccaaa tactttgtag caattaccgc atccgaatct cccggtttcc ctgaacttcc
    25561 cataggacaa tccgcaaaca ttacatttga tatcattcac catttcggtc ttaatatgag
    25621 atgctcctcc tgaattcaac aatccggcca ataaatcatt caccgaaaag cttgtgctta
    25681 aactcagcag gttcttctgc atggcacact gctcacacaa atgcatatcc gactttttcc
    25741 cattctcaac ctttgttata tgaaccgctg ccggcctttt cccgcattca tcacaaatca
    25801 aaacaataac ctcctaacga agtaatctaa accagtgctg caacaattac agccttcaat
    25861 atctctgccc tcaaattgtc cctgtatggt tttcccacct tggagagggt actgtcatcg
    25921 gttgctgcct taagaatatt cacagtatta cagtcaatca gcttctgctt gtacaaagct
    25981 tccgccaggg caaaagcccc ttccttgttg atgctgtccc ctatactggt ggaaagcata
    26041 ttcatcagat aatcatcctt gtcaatgtca atacgggtga tcttaataca gcctccgccg
    26101 cccctccggg attctatgaa ataacctctt tcggtagtga accttgtcat cagcacatag
    26161 tttatctgtg atggggcaca gtcaaaataa ttggccattt catttctctg aatctcaaca
    26221 acacccttgc tctcttctat catgtcactt atgaactttt caattaaatc actaatcctt
    26281 gacatatcat cactctttct gactttgact ttctttgacc ttaattctat tatataatat
    26341 ataatatcat ttttacaagt actatataaa aatattatct ttatgcagca taaaagaata
    26401 atgcgacggg gacgcaggga cacggggacg tttc
//
LOCUS       DAOOAB010000036        26040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2471_length_26040_cov_17.551318, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000036 DAOOAB010000000
VERSION     DAOOAB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 26040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26040
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2471_length_26040_cov_17.551318"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="PKU88_08615"
     CDS             complement(<1..2078)
                     /locus_tag="PKU88_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HPX69375.1"
                     /translation="MEKATKTLFLVVLLGCIFALNTYAAEPEEIYTAEQLNDVRNNLA
                     GSYILMSDIDLSDTVWGEVYAGEYGTSGWEPIGSQASPFRGSFDGNGYTITGLFIDKP
                     NVNEVGLFGNTAASATIEGLRLGDVNVTGKMDVGGLVGLNEGIITNCYVTGSVKGTAE
                     EIGGLVGYHKNAGTITNSYSACAVEGTHFLGGLIGRNEAPISNSYATGDVYGILAGTS
                     SAVGGLVGRNADGIINSYATGNVTGGSNVGGLIGYNWSDMNVEYSYATGAVEGTTKVG
                     GLIGSINVNGTGTVLNSYYNSCTTGQTEDDSGNGEPKTSTEMVQTDTFIDWDFTNTWN
                     INEWESYPRLKWSSHVPAPPAATAVTVSSASVELTYGGGPTTLTAAVEPVCAIQKVTW
                     STSNSDVATVDENGVVTPIGAGEATITATPVSGDVVGETAVTVGKKELTVSGTFTAGD
                     KVYDGSTTAVIDASSLTLSGVVGGDNVELSTSAVFANKNVAENITVNLTSDNSLIGDD
                     AGNYTLSLTGAPISTANITAMELTVINAAAQDKVYDGTADAIIDGAQLSGVVDGDSVT
                     LEDHTVGIFAQATVGTGIGVTTNMTITGDDADNYTIAQPGLLTANITAKELTVINAVA
                     QEKVYDRTTDADIINAELSGVVDGDSVTLENHTVGTFAQAAIGTGIGVTTNMTITGDD
                     VENYTIAQPVL"
     gene            2517..2981
                     /locus_tag="PKU88_08620"
     CDS             2517..2981
                     /locus_tag="PKU88_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPX69376.1"
                     /translation="MPCPMMGCEADEYNSMELQDYGPEPFVVNIEEASKQNNNFRTAL
                     WTGRHLQLTLMSIKVGGDIGLEMHPDLDQFIRIEEGQGVVKMGERKDRLNFQRKVYDD
                     FAFIIPAGTWHNLINTGNIPIKLYSIYAPPQHPKGTVHKTKADAEAAEKKRG"
     gene            complement(3006..3638)
                     /locus_tag="PKU88_08625"
     CDS             complement(3006..3638)
                     /locus_tag="PKU88_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69377.1"
                     /translation="MNKAKLKEAEERFLLKYPGGFSDPLMLEIAKKHKVDKMHKAAQE
                     SFEAENFDDPDKIVDSMSRIVGQSSLVSVFEKPKFRDLVKEISPGERALLSQGLREFL
                     HGDQASGFRLMTGLLDEYKLAKWPLLTVCPVYYRPDVEVFIKPTTVKGVIEYFELEGF
                     KYSPKPTYDFYRAYREQINRIKKEVDASLQIDNTAFCGFLMMSLEIPSYR"
     gene            complement(3635..4225)
                     /locus_tag="PKU88_08630"
     CDS             complement(3635..4225)
                     /locus_tag="PKU88_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HPX69378.1"
                     /translation="MIYRNAVIKDIPAISELQKKYHIATISEEDKPDGFVTTLFTEEQ
                     FKELIEKENGIAVAYDGDKIAAYAMAASWEYWSKWPIFQYMIEDLPNIEYMGQILSVK
                     NSYQYGPICIDKDYRGTEVLPKIFDFSRIQMKNRYPILITFINHINTRSYNAHTKKIG
                     LDVIKSFVFNNNNYYELGYDTSKKVRTIPENGGDRT"
     gene            complement(4249..4638)
                     /locus_tag="PKU88_08635"
     CDS             complement(4249..4638)
                     /locus_tag="PKU88_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPX69379.1"
                     /translation="MYEMKQEYFTGIKFIDDEHTRLFAIANECYELLTNQFLPDKYDY
                     IVNIINELKDYARYHFKHEEEYMGSIGYKRILSQKVAHNDFIEKIDEYDSEKIDENQK
                     ESLLELLDFLNTWLIEHILKKDKQIVE"
     gene            complement(4657..6141)
                     /locus_tag="PKU88_08640"
     CDS             complement(4657..6141)
                     /locus_tag="PKU88_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPX69380.1"
                     /translation="MNKIGFSSEKYLQMQSEKIKERIEFFGGKLYLEFGGKLFDDYHA
                     SRVLPGFEPDSKIKMLLQLKDKVEIVIAISADDIERSKRRGDFGITYDQDIMRLIDAF
                     REIGLYVGSVVITRYRPNFSTDSFQKRLNNLGIKVYRHYPIADYPAAIPLIVSEDGYG
                     KNDYIETARSLIVVTAPGPGSGKMATCLSQLYHEHERGIKAGYAKFETFPIWNLPVKH
                     PVNIAYEAATTDLNDVNMIDPFHLDAYGITAVNYNRDIEVFPVLNTILDKILGKSPYQ
                     SPTDMGVNMAGFCITDEEAVCRASEQEVIRRYYNTRCAQRQGLAEKAEVYKLELLMNT
                     LGITTECRPAVKAALKRAEATDGPAVALQLHDGRIVTGKTTSLLGAAAAVMLNALKEL
                     GGINHDMHLISPIVIEPIQALKLKHMGNHNPRLHTDEILIALSICATTNPTAELAIQQ
                     LSKLRGCEAHSTVILSRVDENVFQKLGINITCEPQYQSKRLYHK"
     gene            complement(6365..6784)
                     /locus_tag="PKU88_08645"
     CDS             complement(6365..6784)
                     /locus_tag="PKU88_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69381.1"
                     /translation="MIVKPPNIVMIVQYDYIQPGDNAKEVGQVAKGDAEREKNGDVIT
                     FSRKKVMEEDGTYLVQTIRKLNPINERATDRGIAWFERFNNNELEIIKAYFEPDVNFT
                     YDSIAGKADATPESMAEGYKKATKMVVKDGKAEMTKY"
     gene            complement(6781..7011)
                     /locus_tag="PKU88_08650"
     CDS             complement(6781..7011)
                     /locus_tag="PKU88_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005397819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPX69382.1"
                     /translation="MKKAAIQLETLTCPSCMLKIENAVKSLEGVERESVSVSFNSSKV
                     KLDFDDEKISIKDIENAINKLGYEVKKSQVKG"
     gene            complement(7051..8904)
                     /locus_tag="PKU88_08655"
     CDS             complement(7051..8904)
                     /locus_tag="PKU88_08655"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPX69383.1"
                     /translation="MQRFILSKKNHIALISAILITIGFTGELGFKNEAVAVWSLTAAS
                     VLGAAPIAIQAYQALRVKVVSIDVLVTIAVIGAFLIKNLEESAVVTFLFLFGSFLEQR
                     TLNKTRSAIKELTEMAPESALKQMESGEFEEVDVDEVDVGDILLVKTGAKVPVDGTVL
                     TGEGHVNEASITGEAVPAGKEKGSQVYAGTILENGTIQIVADRVGEDTTFGRIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLSSIVWLISRDIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVIHDFSKVDTIVFDKTGTLTIGNPKVADKEIYADNAD
                     EVLGYLASVESESGHPLAKAVAEYIGDTKAYTVEKTDVVKGGGIVAQAEGHRVAVGNA
                     ALMEQEGVHLDEKARADIARFEKNGNSLVLTSVDGELKALMGIRDQIRPGVKEDLQKL
                     KKLGVKNLVLLSGDNQGTVDLAARELGLTEAHGHMLPEDKSTYIKELQSKGQIVAFVG
                     DGVNDSPSIALAQIGIAMGSGTDAAIETSDIVLMNSDFSRLPHALGLTKATANNMRQN
                     IVIAVGVVLVLLASVFFSEWMNMSIGMLVHEASILAVILNGMRLLSYRLRY"
     gene            complement(8950..9252)
                     /locus_tag="PKU88_08660"
     CDS             complement(8950..9252)
                     /locus_tag="PKU88_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HPX69384.1"
                     /translation="MSNRTIFDEIRKNLFPRLEQYVPVVARVHGGNHPEFHEVRRLFD
                     EIIKKTKKAAPEKPELSEEFARLREITNNYTVPEDVCESYEAVYNMLAEADKAYQA"
     gene            9435..10250
                     /gene="nagB"
                     /locus_tag="PKU88_08665"
     CDS             9435..10250
                     /gene="nagB"
                     /locus_tag="PKU88_08665"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPX69385.1"
                     /translation="MDIIVRENYDELSKYSAKLIARLVRSKPDCVLGLATGKTPIGTY
                     KELIRMHKEEGLDFSQVRTFNLDEYYGIGIDLDKPYESDQSYARFMHEELFKHINNKK
                     ENIHIPDGLTKKPEEFCKWYEEEIKKAGGIDLQLLGIGGDGHWAFNEPGSSLASRTRI
                     QALTQQTLDDNYESFYKKAGINRAHMPHFAITMGIGTILEAKNIIMLANGIKKADVVA
                     KCIEGPVTSQITASAIQLHSGGITVVLDKDSSSKLKGLHHYMHVEKLKQEYGF"
     gene            complement(10260..11159)
                     /locus_tag="PKU88_08670"
     CDS             complement(10260..11159)
                     /locus_tag="PKU88_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69386.1"
                     /translation="MDKIIEILENQGPLTGKELLEKTGMDEFTAWKNCNSSERIVTKT
                     IGTRYLRLDRLVAGYARLSPSIIREFFGYTVIGTEKNLPDILRKAEYLSQEIIRISKN
                     KFELAKETITKIVESQAASNIIKGGACFIIAGDVAYEMSHLEPRPEVSTGELVKGSDL
                     DIVAVTQGLPGNIINALDTSIYEHKYYLLKNPSYNEEIDYIVKDISKVKEQLKFDSFE
                     SMVASKILYEGKFLYGNFDIFKQIKKMLIDEGIPEKIRVLEEKALLNRNNAKTQLLKY
                     EGKLTDEESMKLFYTKEEKEEFF"
     gene            complement(11143..13692)
                     /locus_tag="PKU88_08675"
     CDS             complement(11143..13692)
                     /locus_tag="PKU88_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HPX69387.1"
                     /translation="MEKLVIGILAHVDAGKTTLSESMLYVSGKIGKPGRVDNKDAYLD
                     TYELERARGITIFSKQAVLEIGETQIILMDTPGHADFSAEMERTLQVLDYAVLVISGA
                     DGVQGHTKTLWRLLDIYQIPVFIFVNKMDQKGTDKDKLMEGMKKQLNDGCIEFGYITA
                     AEFYEQLAMCDDIIMEAYLKTGHIEAEQIRKAVRRRKVFPCFFGSALKLEGIGQFMQG
                     IAKYATAPCYPDEFGAKIFKITRDEQGNRLTHLKITGGKLKVKDVLAGGIRKEKVNQI
                     RIYSGQKYEAVSEAEAGSVCAVTGLSRGKPGEGLGIEAAAPAPVLEPVLIYRILLPEG
                     SDPRAMMPRLRQIEEEEPELNIYWDEQMQEIKVRIMGEVQLEILQSLIQSRFGVPVSF
                     DAGGIVYKETIADVVEGVGHFEPLRHYAEVHLLLEPGEPGSGLQFGTECSEDILGINW
                     QRIILTHLQEKEHIGVLTGSVLTDVKITLVSGRAHNKHTESGDFREAANRAVRQGLRS
                     AESVLLEPYYAFQLELPEKMIGRAMSDIEKMHGTGELLQTDGETAVLAGSAPVIEMRN
                     YQKDVTAYTKGMGRLFCSLKGYGPCHNTEEVIASIGYDPERDAENPAGSVFCTHGAGF
                     SVAWDEVKNYMHVESYIKKRDDFSGEAATEKAASEEGRISLEEIDQIINKTYYGNQGK
                     KSAWKRLKTTRENYCGPVDRVRREKEPKEEYLLVDGYNIIHAWPELKELAEVDIGSAR
                     MSLLDSLSKYQSVRQSNVIVVFDAYRVQGHREEMTDYHNIRLVYTKEAQTADQYIEKF
                     ARDNRDKYDITVATSDGLQQIIVRGAGCSLLSARELKIEIERKEKSYGQDN"
     gene            13881..14321
                     /locus_tag="PKU88_08680"
     CDS             13881..14321
                     /locus_tag="PKU88_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX69388.1"
                     /translation="MKGYYTVQQISDMLDIHPKTIQRYIREGKLRASKVGKGWRITGH
                     DLSTFVESNGDDGSCSGNRPVHCISASSVIDITAGGKEDAIRIMNTLTAALNSKPPEY
                     GQSSMQSQYIEGENMVRVTLWGDIRFMAVMMDTIASLTEHNEKE"
     gene            14327..15157
                     /locus_tag="PKU88_08685"
     CDS             14327..15157
                     /locus_tag="PKU88_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPX69389.1"
                     /translation="MKKSVFKTEAGRDKVRAYYNRVLDQFPFGQRYVETTFGRTFMLT
                     AGHESNPPIILLHGSCSNSAFWFPEIMALSNYFRVYAVDIIGEAGNSEEYRPDLCSNA
                     FALWMKDVLDTLGLERAALIGNSLGGWMALKFATAYPERVSKLILIASGGLAQIRPQF
                     LINADQTRQTNGTVPVSPSIMGEQSIPKEVLDFMNLIVENYNPIRELPVFADEQLLRL
                     NMPILFIDGENDAIIDAEKSAQRLSRLIPSAVIHLLPNCGHVVANSAEYIIPFLMKTH
                     "
     gene            complement(15167..15259)
                     /locus_tag="PKU88_08690"
                     /pseudo
     CDS             complement(15167..15259)
                     /locus_tag="PKU88_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
     gene            complement(15425..16243)
                     /gene="fdhD"
                     /locus_tag="PKU88_08695"
     CDS             complement(15425..16243)
                     /gene="fdhD"
                     /locus_tag="PKU88_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPX69390.1"
                     /translation="MECSYNSNAVEIEVIKVNGSESKIKNEQVVTERALTVYLNGQEF
                     VTMVYSPGHEKELVTGFLALEGIIKRPDQLVKFSYDMQEGLAWADTAEKSILGETLFL
                     KRYMTSCCGKGRTSFYFANDARLAQKIDNPLQITSKDVSYYINLMEEKSELFRRTGGV
                     HGGALASDGQLQYFAFDIGRHNVLDKLYGQAFLEGIDISRKIIVFSGRISSEILIKTA
                     KMGCPILIGVSAPTDLALDLAKELGITVIGFARQERMNLYTHPERIIISEERSF"
     gene            complement(16379..16600)
                     /locus_tag="PKU88_08700"
     CDS             complement(16379..16600)
                     /locus_tag="PKU88_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69391.1"
                     /translation="MRGFHTVQSDSTVYGYYAWNSLILVGKAEGNKLSGHIYDGIFEI
                     RMNPDGKSYSGFIFDNSAAEAGYWTGIRK"
     gene            complement(17086..17478)
                     /locus_tag="PKU88_08705"
     CDS             complement(17086..17478)
                     /locus_tag="PKU88_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPX69392.1"
                     /translation="MTHFKQLSEVLRNYYDIPDQELDKINSLFYPCKLKKGEYFVRSG
                     YMADSIGFIVTGLLRYFYTDKNGVEYTKAFAAENDFAVSYASMLLKKPSAFQLRHWKI
                     PNCFASIIQTGRSCWTMYHVGILSLERL"
     gene            complement(17620..19398)
                     /locus_tag="PKU88_08710"
     CDS             complement(17620..19398)
                     /locus_tag="PKU88_08710"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPX69393.1"
                     /translation="MKNNIFNKKYILIFLLCMILGFSAYVDIFTTLDSKMQDSFLRKS
                     GPVDTEIVLVAIDDQSLEDLGRFPWPRYVHAELLRILSQAQSAAIGIDIIFSEAADDP
                     EEDLALAEAIREAGNVVLPVYAGLEGFSARQGAIKTDNLVVPFPDFPDLFITGHINTF
                     PDPDGIIRKTALYLDYQGQTVPSFAWRIYEQYCKSKGLPAPDLNRLPLDPIKRLEINY
                     AGIPGDYEHVSYSQVISGEVPPEFFRGRIVLVGSYTVGIGDFYFTPLAPEVPMYGMEI
                     HANIIQNLLRGSYLQRAPFSASLLAIVIFGAAAAFGFTRLNPQKGVLLLAVLAACYLA
                     VARLMYDKGYLLSLLYPLLLLALGYLVALAANYISELLERRRITGVFSRYVAPQVVDK
                     LLEGGEKALQLGGSRREITVLFVDIRGFTPLSEAAAPEEVVEILNEYLTLVAQCIFKY
                     GGTLDKFIGDAAMAMFNAPLDLEDHCLKAVQAAWAMKEGATPLQQRLEEKFGRTVQFG
                     IGVNTGPAVIGNIGASFRMDYTAIGDTVNTAARLESNAKPGQILISQDVYEKVKERVA
                     AANLGEIKVKGKAQGVNVFQVDGVHQ"
     gene            complement(19413..20900)
                     /locus_tag="PKU88_08715"
     CDS             complement(19413..20900)
                     /locus_tag="PKU88_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPX69394.1"
                     /translation="MSIANAKLGDHLEELLNIGIALSAEKNHNKLLERILKEARRITN
                     ADAGTLYLCEQDHLVFKIIHNDTLGIYQGGQGEPVDLPPVPLDPANVSSWAALHKKSV
                     NIPDVYAADNFDFSGPKKYDAITGYRTGSMLVVPLENHEGEVNGVLQLLNAKNEKSQI
                     IPFAPELEKIVFSLASQAAVSLTNMQYIQEIDNLFHSFVQVMATAIDARTPYNVNHTR
                     NIARLAEKFAAYIDSLAGGPFSSEKFPESRTAQLVMAAWLHDIGKVAIPLNVMDKPSR
                     LGERIEYVRQRLELVENRLIRSFLEKKAAGAAGREQEEGHRQQLDRLSQARELIEKAN
                     HPATFVDQDLVRELSELAQWEPLPGVRLLMDEEFKCLSIPKGTLTAEERKVMESHVAV
                     AQRMLENIRFNSRYKDVPAWALSHHELLDGTGYPQGLKAGEIPLEARILAVLDVYDAL
                     TASDRPYKKAMPSNAALNILEKMAEEGKLDNLLVAAFKESRIWEE"
     gene            complement(20897..21724)
                     /locus_tag="PKU88_08720"
     CDS             complement(20897..21724)
                     /locus_tag="PKU88_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX69395.1"
                     /translation="MTLSDFTITFWGVRGSRPVPGPRTVIYGGNTPCVEVRIGKRLII
                     LDAGTGITNLGSKLMQEPGPVIGDIFITHVHWDHIQGFPFFQPAFRIGNSFCLYGERK
                     GNVSFAALIKRTMRHPHFPVPLEHMGAALHFTEIEPGDMPELGDGITVRTAANNHPDG
                     GISYRIQYGNKSCCYVTDYEHGPEKAQALLELCAGTDLLIYDACFTAAEYPGYQGWGH
                     STWEEGLALLEATGAGRLILFHHKPERSDEEMAVLETNITGQYPMVKAAREGMVISL"
     gene            complement(21776..>22519)
                     /locus_tag="PKU88_08725"
     CDS             complement(21776..>22519)
                     /locus_tag="PKU88_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69396.1"
                     /translation="PAVAVNTSNGDYLIAWEEEAGGDLQDDIYGQLLDQDGVQIGEKQ
                     TLVSMDWYQSDSVLAYSAAGNKYLLAFVDSNDILGRYIGADGVPEGAIQEIALDQNRP
                     DEEQFDPDVVADSDGRILVVWGDNRNEDWISNIYGRFFNTDGNMEDEFIVYQSEVDQY
                     EPSAAINTADGSCLVAWRDAYDRLYGQKFTADGTGIGEPVEIDSDGEGFGNAAIVYNP
                     EENNYLAVYEDYNSYPRKIKYKILYPQQP"
     assembly_gap    22520..22619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22620..25632)
                     /locus_tag="PKU88_08730"
     CDS             complement(<22620..25632)
                     /locus_tag="PKU88_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPX69397.1"
                     /translation="MKRISWLLIAVLLLSVVMPGINLAGNLVVDADTLRVAKITAAQG
                     DVKILRAGGEKNFPAFKGMGLTQGDTIITGKDGRVTLELAEDKELKIGENSRIMISEL
                     VQSAENNADKTSLNLKAGQVYTNVKGKLSPDAKYEIRTPTAVMGVRGTQFFVSLASGG
                     QAKVVTLEGNVFVTVPQLVTLEDGTTVTREVEIEVQPNQIFVQTGDISDPGRYGLETL
                     TEEVELGLFVLETLQEISIQQPDLINPEILQNLEEKIEKAREEQQEQQERQQEELEQN
                     IRYDNDTSGGSTPTTDRGNDDDDDDDRGNGNEEPGDGDDEDDDLPQLVITSQLNNVQL
                     QLGDELPWTCNIETEPADDVELEVEVEDENIAEAYFDENYLVIEGLESGSTTVTVTAS
                     KEGYLPAVKSFTVTVVTDYEEEGDWDKSIIGDYGEYTDIALDSKGMPHIIFEASSVEY
                     RHWNGSSWQEEEEPQIGSSQGSLAIVKIDGNEYSLISYNLNGCFGYDVFDGEYWSHQS
                     FGDEGVTVAASSIDTIMYNGEYGTGVSYYDEENGDLYFRFDTDPIDIILDSWGDPFPV
                     DGENSDAGKSSSLVFDEDGLAYIAYYDYSSGGEDGSLKLAVINDLLDPELDIMEVDDQ
                     VGYDDGQYVSVSQAVYNESTAIHLAYYDAVNKNLKYAMWLDEEWFIETVDDSEGDVGK
                     YASLVLDYDGNPHISYYAESDSGGALKYARRDPEHEGEWLIEVIDQGEEVGLYSSIAF
                     DNYFSIPHFAYTAMTGGDRVVKHATRQGIMNTIEPGVLSGTAETFEDADFLIESFENE
                     ICYVWREVWGEIDYLGDEDYIISEDGDVLTITLKSDYLNSLEYGIHKIWFYFDYGLPV
                     LLTIDIEENLEAYTLRSNPDLAYDPMNNRYLMVYEKIIGDTNPGIWGRFISADGEQDT
                     EFPISAEIYCNEPKVVYNPVNNNFLVTWSHYPNYEEPLIYAQILDDEGEPYGDFGNFA
                     VCPETTGMGYGQGRPAVAVNTSNGDYLIAWEE"
     gene            complement(25633..>26040)
                     /locus_tag="PKU88_08735"
     CDS             complement(25633..>26040)
                     /locus_tag="PKU88_08735"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HPX69398.1"
                     /translation="DADNNLIWQMPFNPAESKALPNFAPPSEEVQIWVNGVRLEPDAN
                     NKPYIQEGRTIVPLRDFCEKLGYRVDWTADGTITITKGNWQKVFTALDPNLKNNNGYT
                     LAGLRYLAENMGYKVEWVPKYRAVVITDSQGVE"
BASE COUNT         6705 a         6580 c         5075 g         7580 t
ORIGIN      
        1 agtaccggct gagcaattgt atagttttcg acatcatcac cggttatagt catgtttgtg
       61 gttactccaa tgcctgttcc gattgcagct tgagcaaagg taccgacagt atgattttcc
      121 aatgttacac tatcaccgtc tactacaccg ctaagctctg catttataat atcagcatca
      181 gtagttctgt catatacctt ttcctgtgct acagcattaa ttaccgtcaa ttccttggct
      241 gtgatgtttg cagttaaaag ccccggctga gcaattgtat agttatcggc atcatcaccg
      301 gttatagtca tgtttgtggt tactccaatg cctgttccaa ccgtagcttg tgcaaagata
      361 ccgacagtat gatcttccaa tgttacacta tcaccgtcta ctacaccgct aagctgggct
      421 ccgtcgatga tagcatcagc agttccgtca tataccttgt cctgtgctgc tgcattgatt
      481 accgtcaatt ccatggctgt tatgtttgct gttgaaatcg gagcaccggt gagggacaat
      541 gtataattgc cggcatcatc accaatcaac gaattgtcgc tggtcagatt aaccgtaata
      601 ttctctgcca catttttatt ggcgaaaact gctgatgtac tcagttcaac gttatctcca
      661 ccgacaacac ctgacagggt cagacttgaa gcatcaatta ctgctgttgt gctgccgtca
      721 tatactttat ccccagcggt aaaggtcccg ctgacagtca attccttttt tccaacagta
      781 acggcagttt ctccaacaac atcaccggaa accggagtag ctgttatagt agcctctcct
      841 gctcctatag gagttaccac accattttcg tccactgtcg caacatcaga gttgcttgta
      901 gaccaggtaa ctttttggat tgcacatacc ggttccacgg ctgctgtcaa cgtagtcggc
      961 ccgccgccat aagtcaattc caccgaagca gaactaaccg ttactgctgt tgcagcagga
     1021 ggtgcaggca catgactgct ccacttcaag cgcggatagg attcccattc attaatgttc
     1081 caagtgtttg taaaatccca atcaataaaa gtatctgttt gtaccatctc cgttgaggtc
     1141 tttggctcac cattgccgga atcatcttct gtctgaccgg ttgtacaaga attatagtag
     1201 gagtttagca cagtgccagt tccattaacg ttaatactcc caatcagacc acctacttta
     1261 gtggttccct ctacagcgcc tgttgcatag gagtattcaa cattcatatc actccagttg
     1321 taccctatta aaccaccgac gttggaaccg ccggtcacat ttcccgtagc ataagaatta
     1381 ataataccat cggcattacg acctacgaga cctccaacag cactactagt gcccgccaat
     1441 atgccataca catctcctgt ggcataagaa ttgctgatag gtgcttcatt tcgtcctatc
     1501 agtccgccga gaaaatgagt tccctctact gcacatgcag aataggaatt tgtgattgtg
     1561 cctgcgttct tatgataacc caccaaaccg ccaatttctt cagctgtacc tttcactgaa
     1621 cctgtcacat agcagttagt gattataccc tcattaagcc cgactaaacc tccaacatcc
     1681 atcttgccgg taacatttac atctcccagc ctcaagcctt caatggtagc tgatgctgca
     1741 gtattgccaa acaaccctac ttcattgaca ttgggtttgt caatgaaaag tccggtgatt
     1801 gtataaccat taccatcaaa gctgcctcta aagggagaag cttgtgaccc gataggctcc
     1861 caaccactgg ttccgtattc cccggcatat acttctcccc atacagtatc gcttaaatct
     1921 atatctgaca tcaatatgta gctgccagcc agattgtttc tgacatcatt cagttgttcc
     1981 gcagtatata tttcttcagg ttctgccgca tatgtgttaa gtgcaaatat acagcctaac
     2041 aacacgacta aaaaaagtgt tttcgttgct ttctccattt caataatcct ctctttctga
     2101 aatatttatt aaacaattgc aatatgtata tcagtcctac tcatatcagt gcttttaagt
     2161 cttaaatgta actttattat atcaagaatt tgtctgattt tgattgtata gtaacgaatc
     2221 agcaatataa tggtgtgagg tgtcaaaaaa ttagtctggc tcatcttgtc acataaggga
     2281 acttttcact gaagagcagg ggttgcgtat ttgaagcaga aggagcaagg cgaacaggcc
     2341 gttgcccgta tgatgaatat ggctttttca taagaggata cggaactaat agcatgtgaa
     2401 accgggcaca tttttaagta attggcatat gatggtagta ttagtctatg aaaggaatga
     2461 ttagtgtgta taaaaagtac ccatgcccat actaccccgg cttgccgacg tatcatatgc
     2521 cctgccccat gatgggctgc gaagcagatg aatataattc tatggaatta caggactatg
     2581 ggccggaacc ctttgtggta aatatcgagg aggcatccaa acagaataat aacttccgca
     2641 ccgctttatg gacaggaaga catttgcagc ttacactgat gagtatcaag gttggaggcg
     2701 acataggcct ggaaatgcat cccgatcttg accaattcat acgcattgaa gaagggcagg
     2761 gagtcgtaaa aatgggtgaa agaaaagaca gactgaactt tcaaagaaag gtctatgatg
     2821 actttgcctt tattataccg gcaggcacat ggcacaatct aatcaataca ggaaatatac
     2881 ctattaagct atactccatt tatgcacctc cccagcatcc aaaaggtaca gtccataaaa
     2941 ccaaggcaga tgctgaggct gccgaaaaga aacgcggcta aaaatgccgt aagcgataca
     3001 ccatttcaac ggtaggaggg aatttccagc gacatcataa gaaagccgca gaaagctgta
     3061 ttgtcaattt gcagagaggc atcaacctct ttttttatcc ggttgatctg ctcccggtaa
     3121 gcccgataga aatcataggt gggtttcggg ctgtatttga acccttccag ttcaaaatat
     3181 tcaattacgc ctttaactgt agtcggtttg atgaagacct ccacatccgg cctgtagtat
     3241 acgggacata cagttaacaa tggccacttg gcaagcttgt attcatccag caggccggtc
     3301 atcagcctga agccggaagc ctgatccccg tgcagaaatt ccctcagccc ttgggataaa
     3361 agcgcccttt caccggggct tatttctttt acaaggtctc tgaacttagg cttttcaaaa
     3421 accgatacca gggaagactg gccgacaatc ctgctcattg aatcaactat tttatccggg
     3481 tcatcgaaat tctcagcttc aaagctctcc tgagccgctt tgtgcatttt gtccaccttg
     3541 tgcttctttg caatttcaag catcaacggg tctgaaaatc ctccgggata tttaagtaaa
     3601 aatctttctt cagcctcttt aagctttgct ttattcaagt acgatcccct ccattctccg
     3661 gaatggtgcg aacctttttt gatgtatcat agcccaattc atagtagttg ttgttgttga
     3721 acacgaagct ttttattaca tcaaggccaa tctttttagt atgggcattg tatgatctgg
     3781 tatttatatg gttaataaat gttataagta tgggatacct gtttttcatc tgtattctgg
     3841 aaaaatcaaa aatcttcgga agtacttccg ttcccctgta atccttatcg atacagattg
     3901 ggccgtattg ataagagttt tttacggaaa gtatctgccc catatattca atattcggca
     3961 ggtcttcaat catgtactga aaaatcggcc actttgacca gtattcccag gacgcagcca
     4021 tagcataagc agctatctta tcgccatcat aggcaactgc tatgccattt tctttctcta
     4081 taagctcctt gaactgttct tccgtaaata aggtagtcac aaatccatca ggtttgtctt
     4141 cttcgctgat ggttgcaata tggtattttt tctgcaattc tgatattgca ggtatatctt
     4201 taatcacagc atttctataa atcatatctt ttttcatttt ccgccccttt actccacaat
     4261 ctgcttatcc tttttcaata tatgctctat cagccacgta ttcaagaaat ctaacagctc
     4321 caaaagcgat tctttttgat tctcatctat tttttcagaa tcatactcat caattttttc
     4381 aataaaatca ttatgagcga ctttctgcga gagaatccgt ttatacccta tgcttcccat
     4441 atattcttct tcgtgcttga aatgatacct ggcataatcc ttcagttcat ttattatgtt
     4501 cactatgtag tcatacttgt ccggcaggaa ctgattggtc aacaattcat agcattcatt
     4561 cgctatggca aaaagcctcg tgtgctcatc gtctataaac tttattccgg taaagtattc
     4621 ctgtttcatc tcatacataa tacatccctc caaagattat ttatggtaga gcctctttga
     4681 ctggtattgg ggttcgcagg tgatattaat gcccagcttc tggaacacat tttcgtcaac
     4741 cctggataaa ataaccgtgg aatgtgcctc acagcctctc agcttggaaa gctgctgtat
     4801 cgccaactct gcggtggggt tcgtcgtagc gcatatggat aatgctatca gtatctcgtc
     4861 ggtatgcaga cgaggattat gattgcccat atgctttagt ttcaaagcct gaataggttc
     4921 aattacaatg ggtgaaatca ggtgcatatc atgattgatt ccgcctaatt ctttcaatgc
     4981 atttaacatt actgccgcag cagcgccaag caaagaggtg gtttttcccg ttacaatacg
     5041 tccatcatga agctgcagcg ctacagcagg gccatcggta gcttctgcgc gctttaacgc
     5101 cgccttcacc gccggacggc actcggttgt aatacccaat gtattcatta aaagctccag
     5161 cttatatact tccgcttttt cggccaagcc ttgcctttgg gcacatctgg tattgtagta
     5221 cctgcgaata acctcctgct ctgatgctct gcacacagcc tcttcgtcag taatgcagaa
     5281 acccgccata tttacaccca tatccgtcgg gctttgataa ggagatttcc ctaaaatctt
     5341 gtccagtatg gtattcagca cgggaaaaac ttcaatgtcc ctattgtagt taacggctgt
     5401 aataccatag gcatccaagt ggaacgggtc tatcatgttt acatcattca agtcagtggt
     5461 ggctgcctcg tatgcaatat tgacgggatg ctttacgggc aagttccata ttgggaaggt
     5521 ttcaaacttc gcatatcccg cctttatgcc gcgttcgtgc tcatgataaa gctgtgagag
     5581 acaggttgcc attttaccgc ttcccggccc gggggcggta acaacaataa gagatcgagc
     5641 tgtttcaatg taatcgtttt tgccatatcc gtcttcactc acaattaggg gtatggcagc
     5701 gggataatca gcgatgggat aatgcctgta aacctttata ccgagattat tcaggcgctt
     5761 ttgaaaggaa tccgtggaaa agttagggcg gtagcgtgta attacaacgc ttcctacata
     5821 taatccaatt tcacgaaatg cgtcaatcaa gcgcattata tcctgatcat atgtaattcc
     5881 aaaatcgccg cgtcgcttgc ttctttcaat atcatccgcc gagattgcaa ttacgatctc
     5941 caccttatcc ttgagctgca gcagcatttt tattttgctg tcaggttcaa agcccggaag
     6001 tacacgggaa gcatggtaat catcaaatag ttttccgccg aactccagat acagcttacc
     6061 gccgaaaaac tctatccgct cctttatttt ctctgattgc atctgaagat atttttcact
     6121 gctaaatcca attttgttca tatatattct cctgtacaat acttttgtta atataagcta
     6181 ctcatattta ttccaaatta tcttgatgaa taactataag tatttttcct ccttatataa
     6241 taatatattg aaagcatgtc gaaatacaac tgctaatcgc atttttttga taaaaaggat
     6301 tgaaatttta cttcacataa taaaggactg cccctgtagg cagtccttta tcataacagc
     6361 tttattaata ttttgtcatt tcagccttac catctttaac aaccatctta gtggctttct
     6421 tatatccttc cgccatgctt tccggcgttg catccgcttt tcctgctatg gaatcgtagg
     6481 taaagtttac atccggctca aagtaagcct ttattatttc aagctcatta ttgttaaagc
     6541 gttcaaacca agcgatacct ctgtctgttg ccctttcgtt aattggattc agctttctta
     6601 tagtttgaac taaataagtg ccgtcctctt ccataacctt ctttctgcta aaggttatga
     6661 catctccgtt tttctctctt tcggcatcac ccttggcaac ttgtccaact tcctttgcat
     6721 tgtcgccggg ctgtatataa tcatattgaa caatcatgac tatatttgga ggcttgacaa
     6781 tcatcctttg acctgagatt tcttaacctc atatcccagc ttattgattg cattttcgat
     6841 atcttttatt gatatttttt cgtcgtcaaa gtccagtttt actttgcttg aattgaatga
     6901 aacgcttacg ctttctcttt ctactccctc taaagatttt accgcatttt ctatttttag
     6961 catacacgag ggacaagtca atgtttctaa ctgaatagct gcctttttca tttttcatct
     7021 ctcctttaat ctttattaat ctgcaaagca ttaatatctc agtctgtaac taaggagcct
     7081 cataccgttt aaaataacgg ccaatatact tgcttcgtgt actaacatgc cgattgacat
     7141 gttcatccat tcgctgaaaa agacgctggc aagcaggacc agtacaactc ccaccgcgat
     7201 aacaatattc tggcgcatgt tattggctgt tgcttttgtt aaacctaatg catggggcaa
     7261 gcggctgaaa tccgaattca ttaagacaat atccgaagtt tcaatcgctg cgtccgtacc
     7321 gctgcccata gcaatcccga tttgtgccag agctattgaa ggactgtcat ttaccccgtc
     7381 tccgacgaat gcgacaattt gccctttaga ctgcagctct tttatatatg tggatttgtc
     7441 ttccggcagc atgtgcccat gggcttcggt aagtcctaat tcacgtgccg ctaaatccac
     7501 tgttccctgg ttatctccgg aaagaagcac cagatttttg acacctaatt ttttcagctt
     7561 ttgaagatct tcttttaccc ccgggcgaat ttggtcacga atgcccatca gtgcttttaa
     7621 ttcaccatca accgatgtca ggacaagtga gtttccgttt ttctcgaatc gggcaatatc
     7681 cgcacgggct ttttcatcca aatgaacacc ttcctgctcc atcagcgcag cattgcccac
     7741 cgctactcta tgcccctctg cttgagcgac aattccgccg ccttttacaa catctgtttt
     7801 ttcaactgta taagctttag tatcccctat atattccgca actgcttttg ccaacggatg
     7861 gcctgattca ctttcaacac ttgcaagata acctaatact tcatcggcgt tatctgcata
     7921 aatttctttg tcggctactt taggatttcc tattgttaat gtacctgttt tgtcaaatac
     7981 tattgtatca accttgctaa aatcatgaat aacttcactg cccttcagaa ggacaccgtg
     8041 ccgtgctccg ttgccgatac ccgccacatt tgaaacaggc acgccgataa ccaatgctcc
     8101 cgggcatccc agaaccaata ttgtaattgc cagttcaata tcccgtgaaa tcagccatac
     8161 aatgcttgaa agaactaaaa ctgccggagt gtagtatttg gagaatcggt caatgaaacg
     8221 ttcggcttct gattttgaat cctgtgcttc ttcaaccagt tcaataattc tgccgaaagt
     8281 ggtatcttca ccgacacggt cagctacaat ttgaattgtt ccgttttcca atattgtccc
     8341 cgcgtacacc tgagaacctt tctctttacc tgccggaaca gcttcaccgg taatacttgc
     8401 ttcattaaca tggccttctc cggtcaatac cgtaccgtca acaggaacct ttgcgccggt
     8461 tttaacaagc agaatatccc ccacgtcaac ttcatccaca tccacttctt caaattcgcc
     8521 gctttccatt tgcttcaaag cactttccgg cgccatctca gttaattctt ttatcgcaga
     8581 acgtgttttg ttcagtgtcc gctgttctaa aaaggacccg aataagaaca ggaatgtgac
     8641 tacggctgat tcttcaaggt tcttgattaa gaatgccccg ataactgcaa tggtaaccaa
     8701 aacatctata ctgacgactt taactctcag cgcttgatag gcttgaattg caataggtgc
     8761 agcccctaaa accgaggcgg cagtcaagga ccaaaccgcc acagcttcat ttttgaaacc
     8821 cagttcaccg gtaaatccaa ttgtaattaa aattgcacta atcaatgcta tgtgattttt
     8881 cttacttaat atgaatcgct gcattcttct tcaccttctt ccttgaatca ttccggataa
     8941 tcattccgtt taagcctggt atgctttgtc tgcctcagcc agcatgttgt atactgcttc
     9001 ataactttcg catacatctt ccggaaccgt atagttattg gtaatttcac gcaatctggc
     9061 aaattcttcg cttagttcag gtttttccgg tgccgctttt tttgttttct ttattatctc
     9121 atcaaataat ctgcgaactt cgtgaaattc cggatgattg cctccgtgaa cccgggctac
     9181 aacaggtaca tattgttcca gccttgggaa tagatttttc ctgatttcat caaatattgt
     9241 ccggtttgac atgtatttcc cttctttctt tataatttta tctgttttgt tgattccatt
     9301 atatccggaa attaaggaaa ataaattgac ctaaatcaaa attatttatt ttttcaaatt
     9361 ttttcataat cttaatgaaa atatagaaca gaaaatatat aatataagta caatcatttg
     9421 aagagaggtt taaaatggat attatcgtaa gagaaaacta tgatgaacta agcaaatatt
     9481 cagctaagct tattgcccgt ttagtaagat caaagcctga ttgcgtactt ggactggcaa
     9541 ccggaaaaac ccccataggt acctacaagg aacttatcag aatgcacaaa gaggaaggac
     9601 tggatttttc acaggtcagg actttcaatc ttgatgaata ttacggcata ggcattgacc
     9661 tggacaagcc ctacgaatcg gatcagagtt atgcaagatt tatgcatgag gaacttttta
     9721 agcatatcaa taacaaaaaa gaaaatatac atattcccga tggattgaca aagaaacctg
     9781 aagagttctg caaatggtat gaagaagaaa ttaaaaaagc cggcgggata gatttacagc
     9841 ttctaggcat aggcggcgac gggcactggg catttaacga acccgggtca tcccttgcat
     9901 ccagaaccag aattcaagcc cttacacaac aaacccttga tgataattat gagagtttct
     9961 acaagaaagc cggcataaat agggcccata tgccgcactt tgccataaca atgggcattg
    10021 gaaccatttt agaggctaaa aatatcataa tgcttgcaaa cggaatcaag aaggctgacg
    10081 tagttgccaa gtgcattgaa ggccctgtta cttcccaaat caccgcttcg gcaatacaac
    10141 tgcacagcgg cggaattaca gtagtccttg ataaggattc ctcctcaaag ctcaaaggcc
    10201 ttcatcacta tatgcatgtg gaaaaattaa aacaggaata tggtttttag aggaattcat
    10261 cagaagaatt cctctttctc ttcctttgtg taaaacaatt tcatgctctc ttcatcagtc
    10321 agtttacctt catattttaa cagctgtgtc ttggcattgt ttctgttgag caaggctttc
    10381 tcctccagaa ctcttatttt ttcaggtatg ccttcatcta taagcatttt tttaatctgt
    10441 ttgaaaatat caaaattccc ataaagaaat ttaccttcgt aaagaatttt tgaagcaacc
    10501 atcgattcaa agctatcgaa tttcagctgt tcttttacct ttgaaatgtc ttttacgata
    10561 taatctatct cttcattata actggggttc ttaagcaggt agtacttgtg ttcgtatatg
    10621 gaagtatcaa gggcattaat tatattgccc ggcaaacctt gggtaaccgc aacaatatca
    10681 aggtcggaac ccttaaccag ctcaccggtg gacacttcgg gccggggctc cagatgcgac
    10741 atttcatatg ccacatcccc cgcaataata aagcatgcac ctccttttat aatatttgaa
    10801 gctgcctggg attcaacgat ttttgttatt gtctctttcg ccagctcaaa tttgtttttg
    10861 cttatccgga taatctcctg actgagatat tcagccttcc tcagtatatc cggcaaattc
    10921 ttttcagtgc cgattactgt gtagccaaaa aactccctga tgatggaagg tgaaagcctg
    10981 gcatatcctg caaccagcct gtcaagcctc aggtatctgg ttcctattgt ttttgtgact
    11041 attctttcac tgctattaca atttttccac gcagtaaatt cgtccatccc ggttttttca
    11101 agcaattcct taccggttaa aggtccctgg ttttccagta tctcaattat cttgtccata
    11161 ggacttttcc tttctttcaa tttcaatctt cagttctctg gcggataaca gagagcaccc
    11221 cgcacctctt acgattatct gctgcaaacc gtccgatgta gcaactgtta tatcatattt
    11281 atcccgatta tcccgggcaa atttctcaat atactggtcc gccgtttgtg cctctttggt
    11341 atataccaga cggatgttat ggtagtcggt catttcctcc cggtgcccct gaacgcggta
    11401 agcgtcgaat acaacaataa cattgctttg ccgaaccgac tggtatttgc tgagagaatc
    11461 cagcagcgac attctggcac ttcctatgtc gacctccgca agctctttca gttcaggcca
    11521 tgcatgtata atattatagc catccactag cagatactct tctttgggtt ccttttccct
    11581 tctgacacgg tcaacaggcc cgcaatagtt ttcccgggtc gttttgagtc ttttccaggc
    11641 agattttttc ccttggttcc catagtaggt tttatttata atttggtcga tctcttccaa
    11701 gcttatccga ccctcttccg atgctgcttt ttctgttgct gcttccccgg aaaagtcatc
    11761 ccttttctta atatagctct ctacatgcat ataattcttt acttcatccc aagccactga
    11821 aaatcctgcc ccgtgtgtgc aaaatacgga gcccgcggga ttctccgcgt ccctttccgg
    11881 atcatatccg atgcttgcta tgacctcttc cgtattgtgg cagggcccgt aacctttcag
    11941 gctgcaaaac agccttccca tacctttggt ataggcagtt acatcttttt gataattcct
    12001 catttcaata accggagcac tccccgcaag aactgcggtt tcgccgtccg tctgcaataa
    12061 ttcgccggtt ccgtgcattt tctcaatgtc actcatggct ctgcctatca ttttctcggg
    12121 cagctccagc tgaaatgcat aataaggctc cagcaatacg gattccgccg accttaagcc
    12181 ctgccgcacc gcacggttgg cagcctccct gaagtcaccg ctctctgtat gcttattgtg
    12241 ggctctgcct gaaacgagag tgattttcac atccgtaagc accgaccctg tcagaacccc
    12301 tatatgctct ttctcctgaa ggtgggtcaa aataatcctt tgccagttta tgcccaaaat
    12361 atcctcactg cattccgttc caaactgcag gccgcttccc ggttcccccg gctccaataa
    12421 cagatgaacc tccgcataat gccttaaggg ctcgaagtgc ccaactcctt caactacatc
    12481 ggcaatggtt tccttataga caatccctcc ggcatcgaaa gacaccggta ctccaaaacg
    12541 gctttgtatc aggctctgca gaatctcaag ctgcacttcc cccataatac gtacctttat
    12601 ctcctgcatc tgttcatccc agtaaatatt aagctccggc tcctcttcct caatctggcg
    12661 cagcctgggc atcatcgctc tcggatcgct gccttcggga agcaggatcc gataaatcag
    12721 taccggttcc aatacagggg ccggtgcggc tgcttctatt cccaagcctt ctcccggctt
    12781 gccccggctg agccctgtta ctgcgcatac agatcccgct tctgcttcac taactgcctc
    12841 atacttctgt cctgagtaga tacggatttg atttactttt tctttccgga tgccgccggc
    12901 caatacatcc ttaaccttaa gctttccgcc ggtaatttta agatgggtaa gacggtttcc
    12961 ttgttcatcc cttgttattt taaatatctt tgctccgaat tcatcgggat agcaaggtgc
    13021 cgttgcatac tttgcaatac cttgcataaa ttgcccaatg ccttccaatt ttaaagccga
    13081 accaaaaaaa cacggaaata ctttgcgcct ccggacagcc ttcctgatct gctcagcttc
    13141 aatatgccct gttttcagat acgcttccat aataatatca tcacacatgg ccaattgttc
    13201 gtaaaactca gccgccgtaa tatatccgaa ttctatacat ccgtcattaa gctgcttttt
    13261 catgccttcc atcagcttgt ccttatcagt ccctttctga tccattttat tgacaaatat
    13321 aaaaacaggt atctgataaa tatcaagcag ccgccataat gttttggtat gcccctgtac
    13381 tccgtccgca ccgcttatca ccaaaacggc atagtctagc acctggagtg ttctctccat
    13441 ttcagccgaa aaatctgcgt gccccggggt gtccattaaa attatctgag tctcacctat
    13501 ttctaatacg gcctgtttgg agaaaatggt gattcctctt gccctttcaa gctcataggt
    13561 gtctaaatag gcatccttat tatccaccct tcccggcttt ccgatttttc cgcttacata
    13621 aagcatactt tccgataacg tcgtcttgcc ggcatcaaca tgtgccaata ttccgataac
    13681 taacttttcc ataaagaaat aatccttctc attcttcgca tcccttaatt attcgtatat
    13741 gatagtaatt tgcatttaat gctatattta tgatatcatg tattttcttg cggaaacaat
    13801 ggcaaataaa aaaacttctt gacatgtttc tcattattcc ttattattac cttataagta
    13861 actataggga gtgttatgta atgaaaggtt attataccgt ccagcagatt tcagacatgc
    13921 tggacattca tcctaagacc atccagcggt atatccggga gggaaaactg cgtgcatcaa
    13981 aggttggcaa gggctggcgc atcacgggcc atgatttaag cacatttgtc gagagtaacg
    14041 gtgacgatgg atcctgttcc ggaaaccggc ctgtccattg catcagcgcc tcgtcggtta
    14101 tcgatatcac tgctggcgga aaagaggatg ccattcgtat catgaataca ctgacagctg
    14161 ccctgaactc caaaccgccg gaatacggcc aatcgtccat gcagtcgcaa tatatcgagg
    14221 gtgaaaatat ggttcgcgtt acactctggg gtgacatccg ctttatggca gtgatgatgg
    14281 acacgatagc ctcactgaca gaacataatg aaaaggagtg attgatatga agaaaagcgt
    14341 attcaaaact gaggcaggac gggacaaggt tagggcttat tataaccgcg tgcttgatca
    14401 gttccccttc ggacagcgat acgtcgagac caccttcgga cggacattta tgctaaccgc
    14461 cggccatgag tcgaacccgc ccattattct gctgcacggc tcctgcagca acagtgcgtt
    14521 ctggtttccc gaaatcatgg ccttatccaa ttatttcaga gtctatgccg ttgacattat
    14581 cggtgaagcc ggaaacagcg aggaatacag gcccgatcta tgctcaaacg cttttgcact
    14641 ctggatgaag gatgtccttg ataccctcgg ccttgaaaga gccgctttaa tcggcaactc
    14701 actgggcggc tggatggccc tcaaatttgc cacagcttat ccggaacgcg tttccaagct
    14761 gattctgata gcatccggcg gacttgcaca gatccgtccg caatttctta ttaatgcgga
    14821 ccaaacgcgg cagacgaatg gaactgtacc ggtgagccct tccataatgg gagaacaaag
    14881 cattccaaag gaagtcctcg attttatgaa tttaattgtt gaaaactaca atccgattcg
    14941 ggagctgcct gtatttgctg atgaacaatt actgaggctt aacatgccga tactctttat
    15001 agatggagaa aatgatgcta ttattgatgc tgaaaagtca gcgcaaaggc tctcccgcct
    15061 tataccatca gctgtaattc atttgcttcc caattgcggg catgtggtag ccaattccgc
    15121 cgaatatatc attccgtttc tgatgaagac tcattaatat aattgagtca attttcacat
    15181 cataaggaaa ttcgacatat gcaccgtcta tgtcggggac tttctgaatg gcggcctcat
    15241 acttatatgt cttattcatt tcattttcct ctctccaagg cctctccata aaaattgcct
    15301 cctggtatat gctgcctaca taataccaga aggctgccaa tcattcaata acagtttaac
    15361 cttcgtgctt gacactaatt cccctataat taagcagata agttaggggc ctgtcaccta
    15421 tcttctaaaa agaccgctct tcggaaatta taatccgctc cggatgtgtg tatagattca
    15481 tacgctcctg tctggcaaag ccaataactg taattccaag ctctttggcc aggtcaagcg
    15541 ccaaatcggt aggagcggaa actcctatca atatcgggca tcccattttg gccgttttta
    15601 tcaaaatctc agaggaaata cggccgctaa aaacaattat tttccgggag atatcgatac
    15661 cctccaaaaa agcttgcccg tatagcttat ccaaaacatt atgccgccca atgtcaaagg
    15721 caaaatactg aagctgtccg tcagaagcca acgcccctcc gtgtactccg ccggtccggc
    15781 gaaacaattc cgacttttct tccataagat taatatagta actgacgtct ttggaggtta
    15841 tttgtaaagg gttgtcaatt ttttgagcaa gcctggcatc gttggcaaag tagaaagatg
    15901 tccggccttt gccgcagcag gatgtcatat accgcttcaa aaacagggtt tcccctaaaa
    15961 tacttttttc cgcagtgtct gcccaggcca gcccttcctg catatcataa gaaaacttga
    16021 ccaattgatc gggcctctta atgatgcctt ccaaagccag aaaaccggtt accagttctt
    16081 tttcatgccc cggagaatag accattgtca caaactcctg tccgttcagg tagacagtaa
    16141 gtgcccgttc tgttacaacc tgctcatttt taattttgct ctccgaacca ttgactttta
    16201 tcacttcaat ctctacggca tttgaattat aactgcattc cactccgcca tctccttact
    16261 ttattactct attaatatac ttgatcagtt ctcccatgag gattatacca tatagcaccc
    16321 ttcagataaa gtaaacaaaa aaaatgtacc ttgcggtgca tctatgcatt cgggtttatt
    16381 atttcctgat acccgtccaa taaccggctt ctgccgcact gttatcaaat atgaaacccg
    16441 agtaggattt cccgtcaggg ttcatcctta tttcgaaaat accgtcgtat atatgtccgc
    16501 tgagtttgtt gccctccgct ttgcctacca ggataaggga gttccaggcg taatatccgt
    16561 atactgtact gtcggactgg acggtgtgaa agcctctcac tatactctct tcccaataat
    16621 cacatttcca gtttgccttg aagccggagt tatattaaga aaaaaaagag agaatctata
    16681 tagaaaattc attaagaaac tcctcaagta aatgaattta tagaatgctc aattaagtac
    16741 aaacactgcc gtttctgcca ttgctttatt ctctgaatat cgcggattta cacggctcaa
    16801 aatataaaac aacattgttc tttgtgttca ttgtatctac ctcctctata tagcttgagt
    16861 atatagtcag aggttttatt ttcattaata taggttaata attactcatt tttctgcgaa
    16921 ttctgcttaa ggtgaccggt gaaataccta aatatgaagc aatataatgc tgttttagtc
    16981 ttttatgcag catagggtac tgttccataa aatgaatata catgctttga gcatcctcaa
    17041 gaagtaattc tatctcgcgc atctgcttca taatatatac cttttctaca acctttcgag
    17101 cgacaatatt ccaacatggt acattgtcca gcagcttctc ccagtctgaa taattgatgc
    17161 aaagcaattc ggtattttcc aatgcctcaa ttgaaaagcc gaaggtttct tcagaagcat
    17221 acttgcatat gataccgcaa aatcattttc tgccgcaaaa gccttggtat actctactcc
    17281 atttttgtca gtataaaaat accgcaataa tccggtcacg ataaaaccga ttgaatctgc
    17341 catataaccg cttcttacaa aatattctcc tttttttagt ttgcatggat agaacaaact
    17401 gtttattttg tccaattcct gatccgggat atcgtaataa tttcttagta cttctgataa
    17461 ttgtttaaaa tgtgtcattt ccctgatatt cctctccctg aattttcacc attagcagat
    17521 gtgccattaa tcaacttgct gcactcataa ttgacggaca gggtcacccc tgtccgtgaa
    17581 atctcgatac ggtcatatac acaatccata acaaaagctt tactgatgca caccatcgac
    17641 ctggaagacg ttaactccct gggctttccc tttgaccttg atctcgccca ggttggcggc
    17701 tgctactctt tcctttactt tttcgtatac gtcctgggat atcagaatct ggcccggttt
    17761 ggcattgctc tccagacggg cagcggtatt gacggtatcg ccaatggctg tatagtccat
    17821 tctgaagctg gctccgatat tccctatgac agccggtccc gtgttcaccc ctatgccgaa
    17881 ctggacagtg cggccaaatt tttcttccag cctctgctgc aggggagttg ccccttcctt
    17941 catagcccaa gccgcttgga cggcttttaa gcagtgatcc tccagatcca aaggagcatt
    18001 aaacatagcc atggccgcat ccccgatgaa tttgtccagg gtgcctccgt atttaaaaat
    18061 acactgggcc accaaggtca ggtattcgtt gaggatttca accacctctt caggggcggc
    18121 agcctccgaa aggggggtaa aaccccgaat atctacaaac agaactgtaa tctcccgcct
    18181 gcttcctccc agctgcaaag ctttttcgcc gccctccagg agcttgtcca ctacctgggg
    18241 agccacatag cggctgaaga caccggtgat gcggcggcgt tccagcaact cgctgatgta
    18301 attagctgcc aaagccacca aatagcccaa agccagcagg agcaagggat aaagcaaaga
    18361 aaggagatac cccttgtcgt acatcagtcg ggcgacggcc agatagcatg ctgccagtac
    18421 tgccaggagc agcacgccct tttgggggtt gagccgggta aagccaaaag ctgccgctgc
    18481 cccaaaaatg actatggcca gcagggaagc actgaaaggc gctctctgca gatagctgcc
    18541 ccggagcagg ttctgtatga tattggcatg aatttccatt ccatacatag gtacctctgg
    18601 ggccagggga gtaaagtaaa aatctcctat tcccacagta taggaaccca ccaggacaat
    18661 tctgccccgg aagaattcgg gaggcacctc tccgcttatt acctgggagt aactgacatg
    18721 ctcataatcg ccgggaatgc ccgcatagtt tatttccagc cttttgatgg gatccagggg
    18781 cagccggttc aggtcgggag caggcagccc cttgctctta caatactgct catagatacg
    18841 ccaagcgaag ctgggaacgg tttggccctg gtaatccaga tacagggcgg tctttcttat
    18901 gattccgtcc gggtcgggaa aagtatttat atgcccggta atgaataaat cggggaagtc
    18961 cggaaaagga accaccagat tatcggtttt gatggctccc tgcctggcgg aaaagccctc
    19021 caagccggca tagacgggca agacaacatt gcctgcctcc cggatagctt ctgccaaagc
    19081 caggtcttct tccggatcat cggcagcctc actgaaaatt atatcaatgc cgatggctgc
    19141 cgactgagcc tgggacagga tccttaatag ttctgcgtga acatagcggg gccatgggaa
    19201 gcggcccaga tcctccaagc tctgatcatc aatagccacc aggacaattt ccgtatctac
    19261 agggccgctt ttcctcagga agctgtcctg cattttactg tccaaagtgg tgaagatatc
    19321 cacataggca gaaaagccaa gaatcatgca aagcaaaaat atcagaatgt attttttatt
    19381 aaaaatattg tttttcatca gcacaactcc ctttattcct cccagatccg gctctcttta
    19441 aaagccgcca caagcaggtt atccaacttc ccctcctcgg ccatcttttc caggatgttt
    19501 aaggctgcat tggaaggcat ggccttttta taaggcctgt ccgaagctgt taaagcatca
    19561 tagacatcca gaacagcaag aatcctggct tccaagggaa tttccccggc ttttaagcct
    19621 tgggggtagc ctgttccgtc cagcaactca tgatgactca aggcccaagc aggcacatct
    19681 ttataacggc tgttaaagcg tatattttcc aacatccttt gggcaacagc cacatggctc
    19741 tccatgacct ttcgttcttc agcggtcagt gtgcctttgg gtatgctgag gcatttgaat
    19801 tcctcatcca ttaaaagcct cactcccggc aaaggttccc attgggccag ctcggacaac
    19861 tcccggacta aatcctgatc cacaaaagtt gccgggtgat tggctttctc aatgagttcc
    19921 cgggcttggc tgagacgatc caattgctgc cggtgcccct cttcctgttc cctgccggct
    19981 gccccggccg cttttttctc cagaaaagac ctgatgaggc ggttttccac cagttccagc
    20041 ctctgtcgca catactcaat ccgctccccc aatcggctgg gcttgtccat aacgttcaaa
    20101 gggatagcca ccttaccgat atcatggagc catgccgcca tgaccagttg ggctgtccgg
    20161 ctctcgggaa atttttcgct gctgaacggc ccgccggcca agctgtcaat ataggcagca
    20221 aatttttccg ccagtcgggc aatattgcgg gtatggttga cattgtaagg ggtacgggca
    20281 tcaatagccg tagccatgac ctggacaaaa gaatgaaaaa gattatcaat ctcttgaata
    20341 tactgcatat tagttaagga tacggccgcc tgggaagcca gggaaaaaac aatcttctcc
    20401 agttcggggg caaagggtat gatttggctc ttttcatttt tggcattcaa aagctgaagg
    20461 acaccgttca cttccccctc atggttctcc aagggaacca ccagcatgga gccggtgcga
    20521 tacccggtga tagcatcata cttcttggga ccggagaaat caaaattgtc ggcagcataa
    20581 acatcgggta tgttgacact tttcttgtgt aatgccgccc aggaagaaac attggcaggg
    20641 tcaaggggaa cgggaggtaa atcaacgggc tccccctgcc ctccctgata tattcccagg
    20701 gtatcattat gaataatctt aaaaaccagg tgatcctgct cgcataaata caaggtgcct
    20761 gcatctgcat tggtaatcct gcgggcttcc ttcagaatcc gttctaacag cttgttatga
    20821 ttcttctccg ccgacagggc aattccgata ttcagcagtt cctccagatg atcgcccagc
    20881 ttggcgttgg cgatactcat aaactaatca ccattccttc cctggccgct ttgaccattg
    20941 gatattgacc ggttatattc gtttccagaa ccgccatttc ctcatcactg cgctcgggct
    21001 tatggtggaa gaggattagc cggccggctc ccgttgcctc aaggagggcc aatccctctt
    21061 cccaagtgga atgtccccag ccttgatagc cgggatactc ggcagcggta aaacaagcat
    21121 cgtagatgag cagatccgtt ccggcacaca gctccaggag tgcctgcgcc ttttccgggc
    21181 catgctcgta atcagtcaca tagcagcagg atttatttcc gtactgaatc cggtaggaga
    21241 ttcccccgtc aggatgattg tttgcggctg tccgcacggt aatgccatcc cccagttccg
    21301 gcatatcccc aggttcaatc tcggtaaaat gcagggctgc ccccatgtgt tccaggggaa
    21361 caggaaagtg gggatgtctc atagttctct taattaatgc cgcaaaagaa acatttccct
    21421 tcctttctcc gtaaagacaa aagctgttgc ctatgcggaa agcgggctgg aagaaaggaa
    21481 aaccttgtat atgatcccaa tgtacatggg tgataaaaat atccccgatg acagggcccg
    21541 gctcctgcat cagcttggag ccaaggttgg taatccctgt tcccgcatcc aggattatta
    21601 atctcttgcc gattctaact tctacacagg gtgtgttgcc cccgtatatg actgtcctgg
    21661 gtcccggcac cggccgggat cccctgacac cccaaaaggt tatagtaaaa tcactcaaag
    21721 tcataccgtt acctcactaa tttttctatc agcgggcagc tgctgcggtt tataattaag
    21781 gctgctgcgg atacagtatt ttatacttta ttttacgcgg gtagctatta tagtcttcat
    21841 aaaccgccag gtagttgttt tcttcagggt tatagactat tgcagcattt ccaaagcctt
    21901 ctccatcaga atcaatttca acaggctccc caattccggt accatctgca gtgaactttt
    21961 ggccatataa cctgtcgtat gcatcccgcc atgccaccag acaggagccg tctgcagtat
    22021 taatagccgc actcggctcg tattgatcta cctctgactg ataaacaata aattcatctt
    22081 ccatatttcc gtcggtattg aagaacctgc cgtatatatt gcttatccag tcttcattgc
    22141 gattgtcccc ccagaccacc aggatacggc cgtcagaatc cgccacgaca tcaggatcaa
    22201 attgttcttc atcaggccga ttttgatcaa gggcaatctc ttgaatggct ccttctggca
    22261 ccccgtctgc cccgatgtac cgacccagga tatcattgct gtccacaaag gccagcagat
    22321 acttattgcc ggcagcactg taggcaagaa ccgaatcaga ctggtaccag tccatgctta
    22381 ccagggtctg cttttcgcca atctgcacac catcctggtc aagaagctgc ccgtagatat
    22441 cgtcttgcag gtcaccccct gcctcctctt cccaggcaat cagatagtca ccgttggatg
    22501 tattgacagc cactgctggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    22621 ctcttcccag gcaatcagat agtcaccgtt ggatgtattg acagccactg ctggccgccc
    22681 ctgaccatag cccatgcccg ttgtttccgg acacacggca aaattcccaa aatccccgta
    22741 gggttcccct tcatcgtcca ggatctgggc atatatcaaa ggctcctcat agttgggata
    22801 atgcgaccag gttaccagga aattattatt cacaggatta tatactactt taggctcatt
    22861 gcagtaaatc tccgcggata ttggaaactc agtgtcttgt tcgccgtcag cgcttataaa
    22921 cctgccccat attccgggat tagtgtcacc tatgattttc tcgtaaacca ttaagtagcg
    22981 gttattcatg ggatcataag ccagatccgg gttagacctt aatgtataag cttccaagtt
    23041 ttcctctata tcaatggtta agagaacagg caagccgtaa tcgaagtaaa accatatttt
    23101 gtggatacca tactccaggc tgttgagata gtcgcttttc agggtaattg ttaatacatc
    23161 accatcttca gaaataatat aatcttcatc ccccaaataa tctatctctc cccacacttc
    23221 tctccataca taacaaattt cattttcaaa gctttcaatg aggaagtcag catcctcaaa
    23281 tgtctccgca gtcccggaca gtacacctgg ctcaatggta ttcatgatac cctgcctggt
    23341 ggcatgcttc actacccggt ccccaccggt catggctgta tatgcaaaat gcggtatcga
    23401 aaaatagttg tcaaaggcta tggagctgta cagccctact tcctcgccct gatcaattac
    23461 ctctatcaac cattctccct catgctccgg atcgcggcgg gcgtacttca gcgctcctcc
    23521 cgaatcactc tcagcatagt aactgatatg agggttccca tcataatcga gaaccagcga
    23581 ggcatatttg cccacatcgc cttcactgtc atccaccgtt tcaatgaacc actcttcgtc
    23641 taaccacatg gcgtacttaa gatttttatt gacggcatca taatatgcca gatgtattgc
    23701 agttgattca ttataaacag cctgagaaac agaaacatac tggccgtcgt cgtatcccac
    23761 ctggtcatcg acttccatta tatcaagttc aggatccagc aggtcattaa ttacggcaag
    23821 cttcagggaa ccgtcctccc cgccgctgct ataatcatag taggcaatat acgccaggcc
    23881 atcctcatcg aagaccaggg aagaagattt tcccgcatcg ctgttttccc cgtccactgg
    23941 gaaaggatca ccccaagaat caagaataat atctattgga tctgtatcga acctgaagta
    24001 caaatcaccg ttttcctcat cataataact gactccggtg ccatactcac cgttgtacat
    24061 gattgtatca atgctggaag ccgcaactgt cactccttca tccccaaaac tctggtgtga
    24121 ccaatactct ccgtcgaaca catcgtatcc aaagcagccg tttaaattat aagaaattaa
    24181 ggagtattca tttccgtcaa tcttaacaat ggctaaagat ccctgactgc tgccaatctg
    24241 gggttcttcc tcttcttgcc aagaactgcc gttccagtgc cggtactcca ccgagctagc
    24301 ctcaaagata atatgaggca tccccttact atccagagct atatccgtat actctccata
    24361 atcgccaata atactcttat cccagtcgcc ttcttcttca tagtctgtaa caaccgtaac
    24421 tgtaaagctc tttaccgcag ggagataacc ctccttgctg gcagttacgg ttaccgtagt
    24481 actgcctgac tccaggcctt ctataacgag gtagttctcg tcaaagtaag cttcggctat
    24541 gttctcatcc tctacctcca cttccagctc cacatcgtca gcgggctcgg tctctatatt
    24601 acacgtccag ggcagttcat cgccgagctg caattggacg ttattcagct gagaagttat
    24661 tactaactgc ggcaagtcat cgtcctcatc atcaccatct cccggttctt cattaccatt
    24721 tccccgatca tcatcatcat cgtcgtcatt cccccgatcg gtggttggcg tgcttccgcc
    24781 cgaggtatcg ttgtcatacc ggatgttctg ctcaagttcc tcctgctgcc tttcttgctg
    24841 ttcctgctgt tcctctctgg ccttttcaat cttctcctcc aggttctgca ggatttcggg
    24901 attaatcaga tcaggctgct ggatactgat ttcctgcaat gtttccagaa caaataagcc
    24961 cagttctact tcctctgtca gggtctccag gccatacctt ccggggtcgc taatatcccc
    25021 tgtctgaaca aatatctgat taggctgaac ctcaatctcc acttcccggg taacggtagt
    25081 tccgtcttcc agggttacca actggggaac agtaacaaaa acattacctt ccaaggtcac
    25141 cactttagcc tgcccgccgg aggctaaact gacaaagaac tgggttcctc tgacccccat
    25201 tacggcggta ggagtgcgga tttcatactt ggcatcgggg ctaagcttgc ctttgacatt
    25261 ggtatagacc tggcctgcct tcagattcag cgaggtttta tcagcattat tctcagcgga
    25321 ctgcaccaat tcactgatca taatccggct gttttcacca atcttcagct ctttatcctc
    25381 ggctaattcc agggtaaccc gcccgtcttt gccggtaatg atagtgtctc cttgggtcag
    25441 gcccatgcct ttaaaggctg ggaagttttt ttcgccgcct gcccgcaata tcttaacatc
    25501 cccctgggct gccgttattt tggcaacccg cagggtatcc gcatccacaa ccaggttccc
    25561 cgcaagatta attccgggca tgaccacgga cagtaaaagc actgctatca gcaaccagct
    25621 aattcttttc atttattcca ccccctgact atccgtaatg acaacagccc ggtacttggg
    25681 cacccactcc actttataac ccatattctc ggccaggtat cgcaaacctg ccaaagtata
    25741 gccgttattg ttcttcaggt ttggatccaa ggcagtaaac accttttgcc agtttccttt
    25801 agttatggta attgtgccgt ctgctgtcca atcaacccgg taccccagct tctcgcagaa
    25861 atcccgcagg ggcactatgg tccgcccttc ctggatataa ggcttgttat tggcatccgg
    25921 ctccagtctg actccattga cccaaatctg aacctcttcc gaaggaggag caaagttggg
    25981 gagggccttc gactcggcag gattgaaggg catctgccaa ataaggttat tgtctgcatc
//
LOCUS       DAOOAB010000037        24168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2727_length_24168_cov_15.151288, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000037 DAOOAB010000000
VERSION     DAOOAB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 24168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24168
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2727_length_24168_cov_15.151288"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PKU88_08740"
     CDS             <1..860
                     /locus_tag="PKU88_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69399.1"
                     /translation="AEEPAEDEGTTGSEPGTGSYDWDQIVINDTVIFRDNYYIRNKDG
                     FYYIFIGAERLKYAFKLRNAGSYTYPEVEVIGGSITAEWSSGENVVLKAEMREGSTEA
                     IVGGKIVDIGMAPYIIAGKMYIPINLFISALEMDVETSSNDNILFIQYKDAFPAEILS
                     GIWSDTGIDLFAAAKDLESGSGNLPSYATAYRFNSDGTYELIKVSAAGINDTIIKQRG
                     KYKVMGNTIMYYDIFETIYKGSPFSLKYRDEPVDRPHYEFVYNYDSKEEIIEIGGFWL
                     NRVQQFPRK"
     regulatory      927..1120
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1230..2138
                     /locus_tag="PKU88_08745"
     CDS             1230..2138
                     /locus_tag="PKU88_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX69400.1"
                     /translation="MKKIIMLLIACITILSLFGCVDTQQKKEFEDAGKIEVTDIKGRS
                     IVLDKIPQRIVSLSPSSTEILFALGAGDRVVGVTNYCDYPEEARKAEKIGDYDEPNME
                     LIRKVQPDVVLAGHVKEEVAEALEKMGIPVIITEAEDFDTIYHSIKLVGQVTGTTVQA
                     EAIVNGMKSKIEEIEAKFKDKEKPTVFYLVWKDPLFTAGSETFINDVIKAAGGINVAE
                     KVEGWANYSAEEMIKDNPDMLIAALHSTDEGMTREDLSGDRLFSLLECVKQGKVYIMP
                     DDNIISRPGPRIVQAIEDMSKVLHGE"
     assembly_gap    2140..2239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2240..3456
                     /locus_tag="PKU88_08750"
     CDS             <2240..3456
                     /locus_tag="PKU88_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69401.1"
                     /translation="QVEMNIGKGSFFCIVGPNGSGKSTLLRVISAALKPQEGTVYLDG
                     RNINGIKHRSVAGMLSFVPQNTSLEFDFKVSDVVLMGRYPYINKLKGETGKDFEIAEK
                     AMEYTNTTHLKDRSFMELSGGERQRVILAQAFAQDTDILILDEPVSHLDLQHQVEILN
                     LIKKMCVDRKLTAITVLHDLNLASAYGDYIVMMKAGAIMKQGTPFETLTADGIKEIFD
                     TDIYVSVSPVGNKPYIYALTKPNIEKKGIRVHVICGGGSGSDIISRLCSEGFELSSGV
                     LAIGDLDWKISKENDMQVAEEVPFVGISGEAYAQNKELAMAADAIVLTGLYLGKSNLK
                     NLELLLEKDLEGKPLFILEDESFAARDYTGGAACRLYEMIKFRNNSILTDSKLLKEEI
                     IKAVETHGESKE"
     gene            3437..4441
                     /locus_tag="PKU88_08755"
     CDS             3437..4441
                     /locus_tag="PKU88_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPX69402.1"
                     /translation="MGKVKNRNWQVFVFLIASLILTLIFATSTGAVKISTAEIIEILA
                     KKDSDIVNASILMDIRLPRVLLAVVLGAGLAAVGGVMQAIFKNPLVDSYTLGMSSGAA
                     LGAVISIITGLNARILGMETTGVFAFIGAVLTLFFVYSLAYTRNRMSINSFLLAGVAV
                     SYFLASVISFLMMLNHDKIEHIVFWTMGSLSMATWNEFFISAALIIPSLAVLMYYTRE
                     LNILTMGEDAAHHLGINTFALKNVLLITCALVVGSVVSTGGTIAFLGLVAPHIIRLII
                     GSDNKRVIPYSALLGSILLVLADTVGRVLIQPAEIPVGVMTSIMGGPFFIFLLRKSKN
                     "
     gene            complement(4602..5627)
                     /gene="ltaE"
                     /locus_tag="PKU88_08760"
     CDS             complement(4602..5627)
                     /gene="ltaE"
                     /locus_tag="PKU88_08760"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HPX69403.1"
                     /translation="MRYIDFRSDTVTQPTDEMREAMAKAIVGDDVYGDDPTVNLLEEK
                     AAEYAGKEAALFVPSGTMGNQLSVMTHTKRGNEIIAEENCHIIQHEVGAVAILSGAMV
                     RTIRGEKGVMNPQDVLKAIRGNDIHYPETGLICMENALSNGTVVPLEVMKEIYTIAKG
                     HNIPVHLDGARLFNAAAYLEVNAAQITQYTDSVMFCLSKGLCAPVGSMLAGSKEFIEK
                     ARKNRKILGGGMRQAGILAAAGLIALEKMSKRIHIDHENAKYLAERLMSIPGIKLNYD
                     DVHIDMVFFDMEETGISSDKLVDQLFKKGIKINGAEGGLMRFVTNNDITREDIDYTIQ
                     CMKEIIK"
     gene            5907..6506
                     /locus_tag="PKU88_08765"
     CDS             5907..6506
                     /locus_tag="PKU88_08765"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HPX69404.1"
                     /translation="MSKNKLKLICVFSLVLIIALYSVLFYAGRLPAAYTAFTENIGRS
                     KVSRQLFEAYNVMDNTFHFQLPDSWSAYEASFAGEEILYHLNFISKDKKIHGFVQVWK
                     LSEPLKQFIEKSKESAVGVIDFKNFDIKEITVDNKNGYLMDYSRANQKGEYIRAYEAF
                     IKGFSNKVYRLSFFVPEKEWKEYYRILFDRIIHLVKISK"
     gene            6667..7443
                     /locus_tag="PKU88_08770"
     CDS             6667..7443
                     /locus_tag="PKU88_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPX69405.1"
                     /translation="MPINLVLCEDKHIILNSIKASLEKAAKQNGIDANICLMTDNPKR
                     IIEYSRQYSQGVNAYFLDINLETDMNGLELAKQIRSHDPHSYITFITGHLELCMTVFK
                     YHIEAFEYLVKPVSYQAIEECLISIDKQYSQYLSNQKYNKDAMIIIRSGICDYRVELY
                     DIIYVESINQKLVVHTRSRNIEFFGYLKDIIKDLNKNGESFYRCHRSYIININHVKEV
                     NYRDSSIIMSNGEKCYMSRQQKSEMRNILGRMGQDYDDII"
     gene            7427..8722
                     /locus_tag="PKU88_08775"
     CDS             7427..8722
                     /locus_tag="PKU88_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HPX69406.1"
                     /translation="MMILYKIFINTIGMVLFAYLAQALYNFQREKKSYAKFIIIASLG
                     ANVLFYLDKYIGAGVRAIAVLLMMMLMSYFVLKLNVLQSIVANITNGIALVIGDISAV
                     FILVKIYGYSVEMIKSNILLSLISDLIIYGSFIIIILLIKFFRQTQEMTDKYKRKNSI
                     RTSLYMLATFIVITVNYSMYINFIGVASQNIILINVAIMWLYLILSLYINFTNSALAL
                     KEQQYDQQQDYIRTIDSLINDFRRLKHSYSNTIYSFYGYIQENDMEGLKAYYTEVADE
                     AKRMDSNLLLALQRIKVYAVFGLLWNKINEAEGRGIEVGIRVTNEIREAGMKLTDLCE
                     VLGNYLDNAIDAAAASKDKKMNITLTDGGGYLTISIENTYEGDVDVKEIQKKGYSTKG
                     NGRGFGLSVTNQVLSGYTNILHNTFAEGGLFKQELVIKK"
     gene            complement(8753..8890)
                     /locus_tag="PKU88_08780"
     CDS             complement(8753..8890)
                     /locus_tag="PKU88_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HPX69407.1"
                     /translation="MKRFLKPLMILISTLALFIASTSTTACYMWVLREPKMPESLIKR
                     D"
     gene            complement(8893..9510)
                     /locus_tag="PKU88_08785"
     CDS             complement(8893..9510)
                     /locus_tag="PKU88_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HPX69408.1"
                     /translation="MEFPERISGKITKAIAAELKDLSEEKLAEIDYGIAAFIANSYKM
                     VFIFILAMLLNIFQYFLIAFVSFGALRTFASGVHAKREWTCLPVSTSLFLGITYLGIT
                     LDLNIYIINFVFILCFAAILRYAPADTEERPLVSRKLRKKLKIMSCITVLVLYAIALY
                     YLDTSASSIITFAVLVESVLILPVTYRLAGSMYGAGMKISNNGEV"
     gene            9767..10579
                     /locus_tag="PKU88_08790"
     CDS             9767..10579
                     /locus_tag="PKU88_08790"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HPX69409.1"
                     /translation="MPESERGNLVIIGGAEDKRADKAILKEVIRLSGGKDSRVALITT
                     ATNSPEESGREYKRLFGELGAGSVVSINITDRETANNLEGLKVLEDATCIFFVGGDQL
                     RISSILGGTEVHRLLKQAYKNGGVIAGTSAGASMMSEIMVVEGKDDEAPRKCTLKMAP
                     GMGLLEGVIIDQHFNQRGRIGRLLAAVAQNPNVLGIGIDEDTAMIVNKKLEFSVEGSG
                     VVTIVDGRGISFTNISEQYQDDPLAITNVKIHILPRGYGFNLIKKQEIIFRR"
     gene            10620..13247
                     /gene="cphA"
                     /locus_tag="PKU88_08795"
     CDS             10620..13247
                     /gene="cphA"
                     /locus_tag="PKU88_08795"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPX69410.1"
                     /translation="MIIVSSHAYQGKNIYSYKPVVKLVVDLGKYADAPTKDIEGFNDA
                     IQELLPGLKKHCCCKGHEGGFIERLEEGTYFAHVMEHIALELQAMLGYDIRFGKTRET
                     GKKGVYNVIYGYENEYAGLEAGRLAFEIIEEILEGELPDVNTRLEEIKKVCIATDFGV
                     STAAIVKEAQQKDIPVIRLGSGSILQLGYGRNQKRIEATLTENSSCVSVDIACNKELT
                     KSILNEYGIPVPEGKVFKNEQGALEYCEKIGYPVVVKPNYGSHGKGVSINLKNPQEVL
                     EAYKIAREYEDTVLVEKYIKGNYYRVLVVGNKVVAASHRISANVTGDGASTLRELIKR
                     ENSNPMRGEGHEKPLTKINIDKVVEQHLKKRNMTLEYVPDDGEIVYLRENDNLSTGGV
                     AIDVTDEVHEQNKKLIALAAGIVGLDVAGVDISTVDISKPLTETGGAIIEINAAPGIR
                     MHHYPYKGTPRNVAKAIVDMLFPEGSKSRIPIVSVTGTNGKTTTSRMIAHIIKRMGHT
                     VGMTTTSGIYINDELIKYGDNTGPISARTVLMDRRVEYAVLETARGGIVNRGLGYDIA
                     DVGIVTNITEDHLGIDDINTLEDLAYVKSLVAEAVKQNGYTVLNADDQYCLDMRERII
                     SNVILFSLSTDNETVKNHVKNGGIAVYSNGSSIFVNKGGVELPFIEVKAIPAAMNGIL
                     KHNISNSMAAIAGAIGLDIPIESIIAGLSTFRCDSTGNPGRFNIHDINGIKVILDYGH
                     NIDGYRVVIDSLKQMKSGRLIGIIGVPGDRTDSSTIAIGNICGNSFDRIYIKEDRDKR
                     GREPGEIAALLEKGCRMGNIKPSEIFIELAEEKALKKAFLDAVPGDIIIVFFEEYQLL
                     MDVIERIKLDLLKIRQIIA"
     gene            13503..13700
                     /locus_tag="PKU88_08800"
     CDS             13503..13700
                     /locus_tag="PKU88_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69411.1"
                     /translation="MDIHSGDTLVFAVDNMTKRWRISKVDNNVVKIFEEDGSYRQMPY
                     TYLIEMHERGYLKVETPAMVW"
     gene            13860..15212
                     /locus_tag="PKU88_08805"
     CDS             13860..15212
                     /locus_tag="PKU88_08805"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPX69412.1"
                     /translation="MKDNKVETGSYLIIIASLVFILICILFDKPLYFGFAGAVAFTVS
                     VLIKKGYDIRTLNSFMLNGIKGCSKLMVIILLTGIAVSIWLASGVVPTLIYYGFEYIK
                     HANYLLVCFAAAAVVAVVTGTSIGTISTIGIALLGIGKGFEIPAHIVLGAVVSGAFFA
                     DRTSPISGIVNITLKTVNIKYRDYIISILKTLMPPAVITVAVYYFLGRGYISVVNPER
                     IAEFQHSIGSSFVVSPFILLLPLAVIILSLFGINIIFNMSIGIFGGAVLSKFLQNSTV
                     SEVIQYIISGYHPRTGIGELNEILRGGGILSMVELLLILSGAVALNSLLEGANMISPI
                     IQGAVRNVKSKVSMICRTAALSSLITIITCDQVLGVIILGKILQNKFETFGISKIKLA
                     RTIADSGTAIAPLIPWNVNAIIILAITGIPTMQYAPYALMCYLLPVITILFSFFKEKP
                     "
     gene            15427..16263
                     /locus_tag="PKU88_08810"
     CDS             15427..16263
                     /locus_tag="PKU88_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPX69413.1"
                     /translation="MSVKIVADSSCDLNEDLKKELGVTLVPLTLEVGSNVFRDDENLD
                     VKSMLKEMAACPTPPRTACPSPSDSIKAYEGEDSVFVVTLSSNLSGTYESAMLAKKMF
                     LEKAGDKFIHVFDSFSASIAETLISIKIFELAKKKCDDLQIVEKVNEYIESMKTLFLL
                     ESLDNLIKNGRMSRVKGGIATLLNIKPVMGATDDGHIKLVESVRGYRRAFMRFVDIIG
                     EQGEKLEDKVIGIAHCNCLERAEEFKKELLKKYKFKDIIIVEAAGLTSVYANQGGIIV
                     AF"
     gene            16401..16988
                     /locus_tag="PKU88_08815"
     CDS             16401..16988
                     /locus_tag="PKU88_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HPX69414.1"
                     /translation="MKRIISAILIVMILSGCSQKPAEPVETAGPAVQPAAEPIVEPEV
                     EPEKTETEQKAEEESNLFDARKAKEGDMVAGLKIIKVQVDSPEMEDYDAYVDFEGEVT
                     VSGTFKHNLNDEFLDHEISFKVDEESEALLPKLAHDQRYVWFMFMNHDEAEKALGAAG
                     TEGKATVTIRNYSINYAHTEIWNTADLVKAEISSE"
     misc_binding    17073..17283
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    17338..17547
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17601..18494
                     /gene="thrB"
                     /locus_tag="PKU88_08820"
     CDS             17601..18494
                     /gene="thrB"
                     /locus_tag="PKU88_08820"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPX69415.1"
                     /translation="MVEVRVPATSANMGPGFDCLGVALSLYNRFQIEESGNGLLIDGC
                     DETYRNEKNLVYVSMQKCFEKIGCNNRYKGLRINFESDIPISRGLGSSAACILGGVLA
                     ANEIGRGNLNKNEILEIASEIEGHPDNVAPALFGGMTASVKDGNRVYYEKIKLPPGLK
                     FCAIIPDFKLSTKEARAVLPDSIPYKDGVYNVGRVSLLIAALSNGNFEMLKLACKDRL
                     HEIYRGRLIGNYNEIVEECNRLNSLCVFLSGAGPTIMAVLKEEETDFCRQMEEYLSKL
                     ECKWIIKELKSDQNGACVVNI"
     gene            18509..19705
                     /gene="dapG"
                     /locus_tag="PKU88_08825"
     CDS             18509..19705
                     /gene="dapG"
                     /locus_tag="PKU88_08825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPX69416.1"
                     /translation="MRIIVQKFGGTSVASGERRNLVADKVICAIEQGYSPVVVVSAMG
                     RKGDPYATDSLLSLINDKDCSVDKREADLLMSCGEIISAVVMAEAFAERGYKARVLTG
                     GNAGIITDDNFGNANVVSVEAEKVLKVLEQGLIPIVTGFQGMTEEGDFTTLGRGGSDV
                     TGALLGKALNAEYVEIYTDVDGIMTADPNIVPNAKIIDRIDYREVFELAYQGAKVIHP
                     RAVEYALEDNIPLIVKNTFSDTPGTVITNLVEYEENLFTGVTSMTNKAMVIVNPSEVF
                     EGGAEAIFKLLERKKISIDVISESQYQKVFIVDSDAASEVEDILKQLCAEYRIINNCS
                     KIFLIGNKASGMEEVMSKLVKTLEVSNVNVLQTATSHKDICCLIGEADNKKALNELHR
                     AFFCAG"
     gene            19741..21030
                     /locus_tag="PKU88_08830"
     CDS             19741..21030
                     /locus_tag="PKU88_08830"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPX69417.1"
                     /translation="MGKVRIALLGLGTVGQGVWSILNTNKDDILRKCGCEIEVAKILV
                     RDPEKPRGVKVPEGIITADVNDILNDSSIKIVIELMGGTEPAKDYMLRAMKCKKHVVT
                     ANKLVLATQGEELFKTAEQERVLFYYEASVGGGIPIIREINESLTANRIDKLVGIING
                     TTNYILTKMTREGMDFNEALREAQEKGYAEADPSSDVDGYDAAYKLAIMSSLSFGTKI
                     DFSSIYREGISNIKAVDIDYANRFGYVIKLLAIGKEADGRLELRVHPAMVPKDHPIAN
                     VNDSFNAIFIKGNAVGDLMLYGRGAGDLPTGSAVVGDIISILRSNIALSALYSFKNGR
                     AQKEVKSAYENVSEYYIRLNVKDQPGILGEIAAVLGRHDVSIVSVTQDIKDEDNVSIV
                     FITHEALEGNVAESIKEISMLDRVNNLENLIRIENFN"
     gene            complement(21075..21150)
                     /locus_tag="PKU88_08835"
     tRNA            complement(21075..21150)
                     /locus_tag="PKU88_08835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21115..21117),aa:Thr,seq:cgt)
     gene            complement(21170..21352)
                     /locus_tag="PKU88_08840"
     CDS             complement(21170..21352)
                     /locus_tag="PKU88_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69418.1"
                     /translation="MDKTTKKSLIWGGVILVEAFILKLVKKSDLWFYISLPIIAGFLI
                     TEFLIDMPQKPKRNLK"
     gene            21611..22282
                     /locus_tag="PKU88_08845"
     CDS             21611..22282
                     /locus_tag="PKU88_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69419.1"
                     /translation="MKYKYKGSLSLIMIAAFIICAAAGMVSCAPAPTPTPTPTPTPAP
                     EPEPAPEEESLIGFYPLKEGNMWEYEGEGMEYASFIQKVIFSSGNRHQVTIDNGGTII
                     ANVLEESEGSIVNIYRSGEEFSGGNLLDKPSNVNIIMLQTPLEKGNFWISEENHYEII
                     NTEADVVVPAGEFKGCIAVKVTFKDQTSNMTYYYKRGIGMVQSEFRTDSGDLITSKLK
                     RYELK"
     gene            complement(22297..23226)
                     /locus_tag="PKU88_08850"
     CDS             complement(22297..23226)
                     /locus_tag="PKU88_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPX69420.1"
                     /translation="MFFKNEESKVMSAFIAVCIIWGSTYLAIRIGVSVFPPELFAGIR
                     FFVAGLLMLIFARLRGLEFPTNLNSIKNQSIVGLFLLLGGNGLVVWAEQWVHSGIAAL
                     LVATAPLFMALIELFIPGRPRIEFKGWLGLLIGFSGVVILVLSSSGVGSINVKGAILI
                     LAASFLWAMGSVYSKSFKPEGSMIVNIGIQMLAGGIALLILGISLGELPRVSISLKGI
                     GALIYLIVFGSLLGYSSYIYILGKWPAAKAGTYAYINPPVAVLLGTLILKEPFTPTVL
                     LATAIILSGVLLVQLSKIKSVDKAVIKEEDPSM"
     regulatory      23354..23471
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            23625..24158
                     /gene="thiT"
                     /locus_tag="PKU88_08855"
     CDS             23625..24158
                     /gene="thiT"
                     /locus_tag="PKU88_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPX69421.1"
                     /translation="MKRASTQMLAEAGVMIAAAQILSYVKIFEAPYGGSVTAGSMIPI
                     IVFSLRWGVKYGLLAGTAYGILQFLLGGAIYSYHVISILFDYVVAFGMLGLAGLFRSN
                     FKGVFTGTCLGVAGRFICHVISGVVVWASYAPEGMNPMFYSIVYNGSYLLPELIITLI
                     IVGILYKPLISNLGKSA"
BASE COUNT         7568 a         4219 c         5833 g         6448 t
ORIGIN      
        1 ctgctgaaga gcctgctgaa gatgagggta cgacaggcag cgagcccggc accggatcct
       61 atgactggga tcagatagtc ataaacgata cggtaatatt cagggataat tattatatca
      121 ggaacaagga tgggttctat tatattttta taggggccga gaggttaaaa tatgccttta
      181 agctgcgcaa tgccggaagc tatacctatc ctgaggttga agttatcgga ggcagcataa
      241 ctgccgaatg gagcagcgga gagaatgtcg tattgaaggc ggaaatgcgg gaaggcagca
      301 ctgaagccat agtcggcggt aaaatagttg atataggtat ggctccatac attatcgccg
      361 gaaagatgta tattccaatc aatctcttca tatctgccct ggagatggat gtggaaacca
      421 gcagcaatga taatatactg ttcatacaat acaaggatgc ctttcctgca gaaatacttt
      481 caggtatctg gtcagataca ggcattgacc tgtttgcagc tgcaaaggat ttagaaagcg
      541 gttcagggaa ccttccgtct tatgcgacag cttacaggtt caacagtgac ggtacctatg
      601 aactgataaa ggtcagtgcg gcaggtatca acgacaccat aatcaaacag aggggcaaat
      661 ataaggttat ggggaataca atcatgtatt atgatatatt tgagacgatt tataaagggt
      721 ctcccttcag cttgaaatat agggacgaac cagtggatag gccccattat gagtttgttt
      781 acaattatga ttctaaggag gaaataatag aaataggcgg attctggctt aacagggttc
      841 agcaatttcc gaggaaataa tgcgagattg aagatgggtc attctattgt aatgctgccg
      901 gcattgtgat agaatcagaa ttaattgaaa agagaaacag ttcaggtgca taactgcagg
      961 taatttctgc agaagcttaa aggggaaatg ggtgaaagtc ccatgcggtc ccgccactgt
     1021 gaaggtgagt atcctagaca tgccactgga gtttctccgg gaaggcctgg gaatacgacg
     1081 aacctgagcc aggagacctg cctgaatctg aagcttgcct acgagagata gggaggtgct
     1141 taatcgaaat ttttgttcct tctatatcag tagaaggaac tttttactta acatgaatca
     1201 tggactatat ttaatgggag gattgtcatt tgaaaaagat tataatgctg ttaattgcat
     1261 gtataacaat actgagcctt tttggctgcg ttgatactca acagaaaaaa gagtttgagg
     1321 atgcggggaa aattgaagtt acggatatta aaggcaggag catagtgctg gataagattc
     1381 cccagagaat agtctccctg tcaccctcca gtacagaaat actttttgct cttggggcag
     1441 gggacagggt tgtcggggta accaactact gtgactatcc tgaggaagcc aggaaagccg
     1501 aaaaaatagg ggattatgat gagccgaata tggagcttat caggaaggta cagcctgatg
     1561 ttgtgctggc tggtcatgta aaggaggaag ttgcagaggc ccttgagaag atgggtattc
     1621 ctgtaattat tactgaagcg gaagactttg atacaatata tcacagcata aagcttgtag
     1681 gccaggtaac cggaactact gtccaggctg aagcaatcgt aaacgggatg aagagcaaaa
     1741 tagaggaaat agaagcaaag tttaaagata aggaaaaacc cacggtattt tatttggtgt
     1801 ggaaggaccc tctttttaca gccggctcag aaacctttat taatgatgtt ataaaagctg
     1861 ccggagggat aaatgttgcc gaaaaagttg aaggctgggc aaattacagt gcggaagaga
     1921 tgataaagga taatccggat atgctgatag ctgccctcca ctctacggat gaaggtatga
     1981 ccagggagga tctgtcaggg gacaggttat tcagccttct tgaatgtgta aagcagggga
     2041 aggtatatat tatgcctgat gataacataa tatccaggcc ggggccaaga atagtacagg
     2101 caatagaaga tatgtctaag gttttgcatg gagaatagan nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnna tcaggtggaa atgaatatcg gtaaagggag tttcttttgc
     2281 atagtgggtc ctaacggatc gggcaaaagt acccttctca gagttatttc agctgctctt
     2341 aagccccagg agggtactgt gtatcttgac ggcagaaaca tcaatggtat taagcatcgt
     2401 tccgttgccg gaatgctttc ctttgtgccc cagaatacct ctcttgaatt tgatttcaag
     2461 gtttctgatg ttgtgcttat gggcaggtat ccgtatataa acaaactcaa gggagagacc
     2521 ggaaaagatt ttgagattgc agaaaaagct atggaatata caaatacaac acacctgaag
     2581 gacaggagct ttatggagct ttccggggga gagcggcaaa gggtgattct tgcacaagca
     2641 tttgcgcagg atacggatat acttatactt gacgaaccgg tatcccatct ggatcttcag
     2701 caccaggtgg aaatactgaa tcttataaaa aagatgtgtg ttgacaggaa gcttacggca
     2761 attaccgtac tgcacgatct taatctggct tccgcatacg gggactatat tgtaatgatg
     2821 aaggcaggtg caataatgaa gcaggggacc cccttcgaaa cactgactgc tgacggcatc
     2881 aaagaaatat ttgatactga tatatatgta tcggtcagcc ctgtaggaaa caagccttac
     2941 atatatgccc tgaccaaacc caatatagag aaaaagggaa tacgggttca tgtcatatgc
     3001 ggagggggca gcggcagcga tataataagc aggctgtgct ccgagggttt tgaactcagc
     3061 agcggagtgc tggctatcgg cgaccttgac tggaagattt caaaggaaaa tgatatgcag
     3121 gttgctgaag aggtaccttt tgtaggaata tccggggaag cctatgccca aaataaggag
     3181 cttgccatgg ctgccgatgc catagtgctg accggattgt atctgggcaa atcaaacctt
     3241 aagaatcttg aactcctgct tgaaaaggac ctggaaggca agcctctgtt tatactggag
     3301 gatgagagct ttgcagccag ggattatacg gggggtgccg catgcaggct gtatgaaatg
     3361 ataaagttcc ggaacaattc aatacttacg gacagcaaat tattgaagga agaaattata
     3421 aaggcggtag aaactcatgg ggaaagtaaa gaatagaaac tggcaggtgt ttgtatttct
     3481 gattgcctct cttatattaa cgctgatttt tgcaacatca acaggagctg taaaaatcag
     3541 tacagcggaa ataattgaaa tactggcaaa aaaggattct gatatagtca acgcctctat
     3601 tcttatggat ataaggcttc cgagggtact gcttgcagtt gtcctgggag ccgggcttgc
     3661 cgcagtggga ggagtgatgc aggcgatatt caagaacccc ctggtggatt cttatactct
     3721 ggggatgtct tcgggcgccg ccctgggtgc ggtgatttca attatcacag gcctgaatgc
     3781 aaggattttg gggatggaga cgacaggagt atttgcattt attggtgctg ttctcacgct
     3841 gtttttcgta tacagccttg catatacaag aaacaggatg tccatcaatt cctttctgct
     3901 ggccggtgtt gcagtgagct attttcttgc atcggtgata tctttcctta tgatgctgaa
     3961 tcacgataaa attgagcata tagtcttctg gacaatggga agcttatcaa tggcaacctg
     4021 gaatgagttt ttcatatctg cagcacttat aataccctct ttggcagtgc tgatgtatta
     4081 taccagggag cttaacatac tcacgatggg agaagatgct gcccatcatt tgggcataaa
     4141 tacctttgca ttgaaaaacg tacttctcat aacctgtgca cttgttgtcg gcagtgtggt
     4201 ttccaccgga gggacgatag cattcctggg actggttgcc ccccatatta tcaggctgat
     4261 tataggctca gacaataaaa gggtaatacc ttactcggca cttctgggat caatactcct
     4321 ggtgctggcg gatactgtcg gcagggtgct gattcagccg gcggagattc cggtgggagt
     4381 gatgacttcg ataatgggcg gccccttttt catattcctt ttaagaaagt cgaaaaactg
     4441 aacaatatca gaaagatgtg acacaatata agacaataag gatggatttc agatcaagcg
     4501 gaatggtgac ggttcaatag atattgtctt caagaatgtg aaaagttata ttaaagataa
     4561 atgaactcgt ccagccgcgg ctgaacgagt tcatattttt gctattttat tatttctttc
     4621 atgcactgaa tggtatagtc gatatcttcc ctggtaatat cgttgttggt aacgaatctc
     4681 ataagtccgc cttcagcgcc gtttatcttt attccttttt tgaataattg atctacaagc
     4741 ttgtctgaac ttattcctgt ctcttccatg tcaaagaaaa ccatgtcaat atgaacatca
     4801 tcataattaa gctttattcc cggtatcgac ataaggcgtt ctgccaagta ttttgcattt
     4861 tcatgatcaa tgtgaatgcg ttttgacatt ttctccaagg caatgagacc cgcagcggca
     4921 agtattcctg cctggcgcat cccgcctccc agtattttcc ggttctttct cgccttttct
     4981 atgaattctt tgctgcctgc aagcatcgac cctacgggag cgcacaagcc cttggaaagg
     5041 cagaacatca cagagtccgt atattgagta atctgtgccg cattcacttc caggtatgct
     5101 gcggcattga aaagccttgc accgtcaaga tgaacaggga tattgtggcc ttttgcaata
     5161 gtatatattt ctttcattac ctcaaggggt acaacagttc cgtttgacaa tgcgttttcc
     5221 atgcagataa gtccggtttc agggtagtgt atgtcattgc cgcgaattgc ttttaaaaca
     5281 tcctggggat tcatgacacc cttttcccct ctgattgtcc tcaccattgc accggacaat
     5341 atcgctacag ctccaacctc gtgctgtatt atatggcagt tttcttccgc aataatctca
     5401 tttccccttt ttgtgtgggt cataactgac agctgattcc ccatggtgcc gctgggtaca
     5461 aaaagagcag cttccttgcc ggcatattct gcggcttttt cttccaaaag gtttacagta
     5521 ggatcatcac cgtatacatc atcaccaact attgcttttg ccattgcctc ccgcatttca
     5581 tccgtaggct gggtaaccgt atcacttcta aaatctatgt atctcaatgc taaaaccccc
     5641 atatatttta tataaattat tatacagcat ttgtattggc tgtacaacat aagaagaatg
     5701 cgacgcgagg acacgggaca cggggacatg gggacacggg acacggggac gtttcgcgtg
     5761 tcccactgga agtgggacag gggaaacgtc cccgtgtccc gcgtccccgt gtccccgcgt
     5821 cgcatcattc ttttgttctt tatcaacata tagcttaata caaatatagt aatctaagaa
     5881 atcagtttta taggaggggg tcaacaatgt caaaaaacaa attaaagctt atatgcgttt
     5941 tttctttagt tttaataata gccttatatt ctgtactttt ttatgccgga agacttccgg
     6001 cggcatatac cgcatttacg gaaaacatcg gaaggtcaaa ggtctcgagg cagcttttcg
     6061 aggcttacaa tgtgatggat aatacatttc attttcaatt gcccgattca tggtccgcat
     6121 acgaagcatc ctttgcaggg gaggaaatac tatatcatct gaatttcatt tccaaggata
     6181 agaagataca tggttttgtc caggtgtgga agctctccga gccgttaaag cagtttattg
     6241 aaaagtcgaa ggaatccgct gtaggtgtaa tcgatttcaa aaatttcgat atcaaggaaa
     6301 taacggttga taacaaaaac ggctatctta tggattacag cagggcaaat cagaaagggg
     6361 agtatataag ggcttatgag gcatttatta aggggttttc aaacaaggtg tacagattaa
     6421 gcttctttgt gccggaaaaa gagtggaaag aatattacag aatccttttt gacaggataa
     6481 tacatttggt aaagattagc aaataggcaa tatttaacag acgcaaaggt cagaacgata
     6541 tatatggaat cgatgccgca gtattttgtt tatgctaaaa gggaaaaaat gttttatgtc
     6601 gaataatcat atagttatga ttattttttg taatggttaa ttctgagcat gttaggaggt
     6661 agtcagatgc caattaactt agtgctgtgt gaggacaagc atatcatatt gaatagtatc
     6721 aaggcaagcc tggagaaggc tgccaaacaa aacggaatag atgcgaatat ttgcctcatg
     6781 actgacaatc ccaaaaggat aatcgaatat tccagacaat actcacaagg tgttaacgca
     6841 tattttcttg atatcaacct ggaaacagat atgaatggac tggagttggc caagcaaata
     6901 aggagccatg acccgcacag ctacataacc tttattacag ggcatctgga attgtgcatg
     6961 acggtgttta aataccatat cgaagcattt gaatatcttg ttaaaccggt atcataccag
     7021 gccatagaag aatgtctgat ttcaatagac aagcaatata gccaatactt gagcaatcaa
     7081 aaatacaaca aagatgcaat gatcataatc aggtcgggga tctgcgatta tagagtggaa
     7141 ctctatgaca tcatatacgt tgagtcgata aatcaaaagc ttgtagtgca taccagaagc
     7201 cgcaacattg aattcttcgg atatctgaag gatatcataa aagatttgaa caagaacgga
     7261 gaaagctttt accgatgcca tcggtcgtac ataatcaaca ttaaccatgt aaaagaggta
     7321 aactacaggg attcaagtat tattatgtcc aatggtgaaa agtgctatat gtccaggcag
     7381 caaaaaagcg aaatgcggaa tatattgggt aggatgggtc aggattatga tgatattata
     7441 taaaatattt ataaatacaa ttgggatggt actctttgca tatttggcgc aggctttata
     7501 caactttcaa agggaaaaga agtcatatgc aaagtttatt attatagcat ctcttggggc
     7561 aaatgtatta ttttatctgg acaaatatat tggagcaggg gttcgtgcca tagctgtttt
     7621 gcttatgatg atgttaatgt cttattttgt tttaaaactt aacgtgctcc aatcaatagt
     7681 tgcaaatata accaatggta ttgctcttgt aataggagac atatctgctg tatttatttt
     7741 agtaaaaata tatgggtatt cggttgaaat gataaagtca aatatattgc tatcattgat
     7801 atcggatttg ataatatacg gttcgtttat aataataatt cttctaatta aatttttcag
     7861 gcagacccag gaaatgacgg acaaatataa gcgaaaaaac agtataagaa cgtcacttta
     7921 catgcttgca accttcatag taattacggt taattattcg atgtatataa actttattgg
     7981 agtggcaagt caaaacatta ttcttataaa tgttgcaatc atgtggcttt atcttatact
     8041 gagcctttac ataaacttta ccaacagtgc cctggccttg aaggagcagc agtatgacca
     8101 gcagcaggac tatatcagaa ccatagacag cctaattaat gatttcagaa ggttaaagca
     8161 cagctattcc aatacaatat acagctttta cggatacatt caggaaaatg acatggaagg
     8221 actgaaagct tattatactg aggtggctga tgaggcaaaa aggatggaca gcaatctgct
     8281 tctggcactg cagcggataa aggtgtatgc ggtgttcggg ctgctgtgga ataagattaa
     8341 tgaagccgag ggcaggggaa tagaagtggg gatccgggta accaatgaaa tccgtgaagc
     8401 gggtatgaag cttacagacc tctgcgaggt gctgggaaac tatcttgaca atgccattga
     8461 tgcggcagcg gcttcaaagg ataaaaaaat gaatatcact ctgaccgacg gtggggggta
     8521 ccttactata agtattgaga atacatacga gggagatgtc gatgtgaagg agatacaaaa
     8581 gaaggggtat tccacaaaag ggaatggacg gggctttggc ttgtcagtta ccaatcaagt
     8641 attatccgga tatacaaata tactgcataa cacatttgca gagggtggct tattcaagca
     8701 ggaactggtg ataaaaaaat gagagcgcat gaagcgctct cattttttag ctttagtctc
     8761 ttttaatcag tgattcaggc attttcggct ctctcagtac ccacatataa catgccgttg
     8821 ttgaagtgct tgctataaat aaagccaagg tacttatgag aatcattaga ggtttaagga
     8881 atcttttcat tttcatacct ccccattatt acttattttc atacctgcac catacatgct
     8941 gccggccagc ctgtatgtaa cgggtagtat taagactgat tcaaccaata ccgcaaaagt
     9001 tatgattgaa gaagccgatg tgtccaaata atataatgca attgcataaa gcaccaggac
     9061 agtgatgcag gacattattt taagcttctt cctcaatttt ctgctgacaa gtggccgttc
     9121 ttcagtatcg gcaggagcat atctcagaat tgctgcaaag cacagtataa atacaaagtt
     9181 gataatatag atattcaagt ccagggttat tcccaggtaa gtaataccca gaaaaagcga
     9241 ggtactgacc ggaaggcatg tccactcacg ctttgcatga actccggagg caaaggtgcg
     9301 cagtgcgccg aaactgacga aggctattag aaaatattga aatatgttta acagcatggc
     9361 taatatgaat ataaagacca ttttgtatga gtttgcgata aatgcggcaa tgccatagtc
     9421 tatttcggca agtttttcct ccgacaaatc cttcagctct gctgcaatag cttttgtaat
     9481 tttcccgctt atcctttccg gaaactccat aatatcaacc ttctttttct tgcctgactt
     9541 agatactaat ttaataataa gcacacatat gttgggattc aatagtttta tacaaaatgc
     9601 ccacattatg atataaaatg tagaaaaatg ccgaattgcc aagaatattg acacttcatc
     9661 aataattaat aatgcagatg ggaaagttaa ttccaatgca ttttttttac ttttattggt
     9721 atattaaata tgttattaaa aattatgaaa gcagggggat acggcattgc ctgaatctga
     9781 aaggggaaac cttgtaataa ttggaggagc ggaggacaag agggccgata aggcaatatt
     9841 gaaagaagtc atcagactgt ccggagggaa agattcacgg gttgccttga ttactacggc
     9901 aactaacagc cctgaggagt cggggcggga atataaaagg ctgtttggag aattgggagc
     9961 cggaagtgtg gtgtcaataa atataacaga cagggagact gcaaataatc ttgagggttt
    10021 gaaagtactg gaggatgcaa cctgcatatt ttttgtagga ggagaccagc ttaggatttc
    10081 cagcatattg ggcggtactg aagtgcatag attactgaaa caagcatata aaaacggcgg
    10141 agtaattgcg ggaaccagtg caggtgcttc catgatgagc gaaataatgg ttgtggaagg
    10201 aaaggatgac gaggctccga gaaaatgtac gcttaaaatg gctcccggta tgggcctttt
    10261 ggaaggagtt ataatagacc agcattttaa tcagagggga aggattggaa ggctgctggc
    10321 agctgtggcg caaaacccga atgttttggg aatcggaata gatgaggata cagccatgat
    10381 agtaaacaag aagctggaat tttcggtgga aggctccggg gtagtcacaa ttgtcgacgg
    10441 gagaggaatc tcatttacaa atatatcgga gcagtatcag gatgatcctc ttgccataac
    10501 caacgtaaaa atacacattt tgccaagagg atacgggttt aatttaataa aaaaacagga
    10561 aataatattt aggagatgaa agaaaatcat ttttctcaaa aacacaagga ggaagtattg
    10621 tgattattgt aagcagtcat gcttatcaag gtaaaaacat ttatagttat aaacctgtgg
    10681 tgaagctggt agtagacctg ggtaaatacg cagacgctcc gaccaaggat attgagggtt
    10741 ttaatgatgc aattcaggag cttctgccgg gtttgaaaaa gcactgttgc tgcaaggggc
    10801 atgagggagg ctttattgag aggcttgagg aaggaacata ttttgcacat gtaatggaac
    10861 atattgcact tgaactgcaa gccatgctgg gatatgacat aagattcggc aagacaaggg
    10921 agaccggcaa aaaaggtgtt tacaatgtta tatatggata tgaaaacgag tatgcgggct
    10981 tggaggcagg caggcttgca tttgaaatca tagaggaaat attggaaggg gagctgccgg
    11041 atgtaaatac aaggctggaa gaaatcaaaa aggtttgtat tgcgacagat tttggggtca
    11101 gtactgctgc aatcgtaaag gaggcacagc aaaaggatat acctgtgata cgcttgggaa
    11161 gcgggagtat tctgcagctg ggatacggca ggaatcaaaa aaggatagag gcaacactga
    11221 ctgagaattc aagctgcgta tcggtggata ttgcctgcaa caaggaactt accaagagca
    11281 tactgaatga atatgggata cctgttccgg aagggaaggt atttaagaat gagcagggtg
    11341 ctttggaata ctgtgaaaaa atcggctatc ccgtagtagt gaagcctaat tacggaagcc
    11401 atggaaaagg tgtatccata aatcttaaga atcctcagga agtattagaa gcatataaaa
    11461 tagcaaggga atatgaagat acagttctgg tggaaaaata tataaaaggc aattattata
    11521 gggttctggt tgtgggaaat aaggtagtgg ctgcctcaca caggatttcc gccaatgtaa
    11581 cgggagacgg agcaagcacc ctgagagaac tgatcaaaag ggaaaacagc aatcccatgc
    11641 gcggagaagg ccatgaaaag cctttgacga agataaacat agataaagta gtggaacagc
    11701 acctgaaaaa aaggaacatg actcttgagt acgttcctga tgatggagaa atagtgtatc
    11761 tacgcgaaaa tgacaatttg agcacagggg gagtagcaat tgatgttact gatgaggtac
    11821 atgagcaaaa caaaaaactg atagcattgg ctgccggtat agtcggcctg gatgtggcgg
    11881 gagttgatat cagcacggtt gatatttcca agcctctgac tgaaacagga ggagcaataa
    11941 ttgaaataaa tgccgcaccg ggtatcagga tgcaccatta tccatataag ggcaccccca
    12001 ggaacgtggc aaaagcaata gtggatatgc tgtttccgga agggagcaag agcaggattc
    12061 caatagtatc agtgacaggg acaaacggaa aaactacgac ttcaagaatg atagctcata
    12121 taataaagcg gatgggacat actgtaggaa tgactaccac aagcggtatc tatataaatg
    12181 atgagctgat aaaatatggg gataacacag gacccataag tgcaagaaca gttctgatgg
    12241 acagaagggt agaatatgct gttctggaga ctgccagggg aggaatagtg aacaggggac
    12301 tgggctacga tattgctgac gtcggtatag tgaccaatat cactgaagac catttgggga
    12361 ttgatgatat taataccctt gaggatttgg cctatgtgaa atctttggta gcagaagcag
    12421 ttaaacagaa cggctatacg gttttaaacg ccgatgatca atactgcctt gacatgaggg
    12481 aaaggattat atcaaatgtt attctgtttt ccttgtcaac agataatgaa acagtaaaga
    12541 accatgtgaa aaacggcgga attgccgtat acagcaacgg cagcagcata tttgtaaata
    12601 aaggaggggt ggagctgccg tttattgaag taaaagctat tcctgctgcc atgaatggaa
    12661 tactgaagca caacataagc aacagcatgg ctgcaatagc cggagcaata gggttggata
    12721 tccccattga aagtataata gcaggacttt caacctttag atgcgacagc accggtaatc
    12781 ccggaagatt caatattcat gatatcaacg gaataaaggt gatattggac tatgggcaca
    12841 atattgacgg ttatagggtg gttattgatt cccttaagca aatgaaatcc ggccgtctta
    12901 ttggaataat cggagtgccc ggggacagga cggactcaag cacaatagcc ataggcaata
    12961 tttgcggcaa cagttttgac agaatctata taaaggagga cagagacaag agaggacgcg
    13021 aaccggggga gatcgcggca ctgcttgaaa aaggctgcag gatgggaaac attaagccat
    13081 cggaaatctt tattgagctt gccgaggaaa aagcactgaa aaaggctttc ctggatgcgg
    13141 tgccgggaga tattattatt gtattctttg aagaatacca attgctgatg gatgttattg
    13201 agaggataaa attagatctg ttgaaaatcc ggcagattat agcatgagag gacacggagg
    13261 cgaggacacg gggacgtttc ccctgtctca ctgaaagcga gacacgcgaa acgtccccgt
    13321 gtcctccccg tgtcctcccc atgtcctcca tgaatatata ttactagtat ttttttttaa
    13381 tatgatactt ttttactata gaatagcatg agctggtagt atataatata ataaaatatc
    13441 aaataatttg acagcattta ccgacatttg cagtattatt atatgtcata ggggtggatt
    13501 gaatggatat acacagtggt gataccttgg ttttcgctgt tgacaacatg actaagagat
    13561 ggaggatatc aaaggtagac aacaatgttg tgaagatctt tgaggaagac ggatcgtaca
    13621 gacagatgcc ctatacatat ctgatagaaa tgcatgagag aggatattta aaggttgaaa
    13681 cccccgccat ggtatggtaa taaagcagtt atagcctatt gttcccaaac aataggtttt
    13741 tttgtttttt atgtcgaaaa atgtagattt ttatataatc atataaagtg cgtattgatg
    13801 atatagttta aaagacgtat agtattattt gaatatactt tttgtgtgtg agagggccta
    13861 tgaaagataa taaagtcgaa acaggctcat atttgataat aatagccagc ctggtgttca
    13921 ttttgatttg catactattc gataagccgc tgtattttgg gtttgccggg gcagttgctt
    13981 ttactgtgtc ggtactcatt aagaaggggt atgatatcag aactttgaac agctttatgc
    14041 taaacggtat aaaaggttgt tcgaagctga tggtaattat tctgcttaca ggtattgcag
    14101 tgtccatatg gctggcctca ggagtcgttc ctaccttaat atactacgga ttcgaatata
    14161 taaagcatgc caattactta cttgtatgtt ttgcagcagc agcggtggta gcagtggtta
    14221 caggcacttc tatcggcacc ataagcacta tcgggatagc attgttaggt atagggaaag
    14281 gatttgaaat acctgcgcat atagtattgg gggctgtagt atccggtgca ttttttgcgg
    14341 acaggacttc acctatatct gggattgtga atattacatt aaagactgta aatataaaat
    14401 accgggatta tattatcagc attttgaaaa ccttaatgcc tcctgcggtt attactgttg
    14461 cagtgtatta ttttttagga aggggatata tatcagtagt caatcctgaa aggatagctg
    14521 aattccagca tagtatcggc agttcttttg tcgtcagccc ttttatactt ttgcttccgc
    14581 tggctgttat aattctgtcc ctattcggta ttaatattat attcaatatg agcataggaa
    14641 ttttcggggg cgctgtttta agcaaatttt tgcagaatag tactgtatct gaagttattc
    14701 aatatattat ttccggttat catccaagga caggtattgg ggaacttaat gaaattctaa
    14761 ggggtggggg gatattgtca atggttgaat tgcttttgat tctgtcaggg gcagttgcac
    14821 ttaacagcct tcttgaaggt gcaaatatga tttcccctat aatccaagga gcagtgagaa
    14881 atgttaagag caaagtatca atgatatgca ggacagcagc tttaagcagt ctgattacaa
    14941 taattacttg tgaccaggtc ctcggagtca taattctggg aaagattttg cagaataaat
    15001 ttgaaacctt tggaatcagc aagataaagt tagccagaac cattgcggat tcgggaactg
    15061 ccatcgcacc gcttatcccg tggaatgtga acgcaataat aatactggcc attacaggta
    15121 taccaacaat gcaatatgca ccttatgcgc tgatgtgtta cctgctgcct gtcattacaa
    15181 tattattctc attttttaaa gagaaaccat gatttaggtc agttgttgat gaagcagcag
    15241 cataatagta tttgatacca gataatatgc atattagtac tatacgctaa agcttttaat
    15301 tgcatatatc gataacttat gctaaaaata gaaatagaat actatatacg tcatgaataa
    15361 gacttgtcac aacataggaa tcaatgcaaa tgttcagtgc gtaacttatg attggaggat
    15421 atgcaaatga gtgtcaaaat agttgcagac agcagctgtg acctgaatga agatttgaaa
    15481 aaggagcttg gcgttacgct ggtacctctt actcttgaag taggcagcaa tgtctttagg
    15541 gatgatgaaa acctggatgt taaatccatg ctaaaggaaa tggcagcatg tccgactccc
    15601 cccagaaccg catgtccttc acccagtgat tctattaaag catatgaggg cgaggatagc
    15661 gttttcgttg ttacattaag ctccaacctt agcggaactt atgaaagcgc aatgcttgcg
    15721 aagaagatgt ttttggaaaa ggcaggggat aaatttattc atgtttttga ctccttcagc
    15781 gcttcaatag cagaaacact tataagtatc aagatttttg aactggcaaa gaaaaaatgt
    15841 gatgaccttc aaatagtaga aaaagtcaat gaatacatcg agagcatgaa gactttattt
    15901 cttcttgaat ccctggacaa cctcataaaa aacggaagga tgagcagggt aaaaggcgga
    15961 attgcaacgc ttctgaacat caagcctgtt atgggggcaa ctgacgatgg gcatataaag
    16021 ctggtagaga gtgtcagggg ttacagaagg gcatttatgc gttttgttga tattataggg
    16081 gagcagggag agaagctgga ggataaggtt attggaatag ctcactgcaa ctgccttgaa
    16141 agagcggagg agtttaagaa agagcttctg aaaaaatata agttcaagga tataattata
    16201 gtagaggctg caggccttac cagcgtgtat gcaaaccagg gaggcataat agttgcgttt
    16261 tagcgggtat aaaaggtaca ttaatgcagc ttgatgtgtg gcagtcaaaa ataggactat
    16321 agtgcgatag tcctattttt tatttctgcc ttaagaaaat ggaataattt tcgaaattat
    16381 ttgtaatgag gggtgataag ttgaaaagga taatatcagc aattttaata gtcatgattt
    16441 tatcgggatg ttcccaaaaa cctgctgaac ctgtggagac agctgggcct gccgtacagc
    16501 ctgcagcaga gccgatagtt gaaccggaag ttgagccgga aaaaactgaa acggagcaga
    16561 aggctgaaga agaaagcaat ctgtttgatg caagaaaagc aaaggaaggt gatatggtag
    16621 ccggtctgaa aatcattaag gttcaagtgg attctcccga aatggaggat tatgatgcat
    16681 atgtagattt tgaaggggaa gtgacggtaa gcggcacatt taagcataat ctgaacgacg
    16741 aattcttgga tcatgaaata agcttcaagg tcgatgagga atccgaggcc ttgctcccaa
    16801 agcttgccca tgaccagagg tatgtgtggt tcatgttcat gaaccacgat gaagcggaga
    16861 aggcattagg agctgcaggt acggaaggaa aggcaactgt aacaataagg aattacagca
    16921 ttaattatgc tcatacagag atatggaata cggctgattt ggtgaaagca gagatttctt
    16981 ccgaataatc tgagtaattc gagaatattt aaaaaaaccc ttgacgagaa tatgaaaatg
    17041 atataaacta ataaaaatag tttatacgac ttataaatac tgtgatcggg acgagtagac
    17101 catgttcggc taacagagac ggagatccgc cgactgaaag atttccttgg caagaatggt
    17161 tgaaaaccac ccgggagtag cattctgaaa taccagtaag aaatgacgcc ggctgcgtta
    17221 aagctttgag tgggtttata ccaatatggg tggaaccgcg ggagtcaggc tctcgtccct
    17281 ttcagggacg ggagcttttt gcatacccgg caccccctaa acaaaattac atatatgtat
    17341 ataacgatga tcgggacgag taaaccatgt tcggctaaca gagacggaga tccgccggct
    17401 gaaagatttc cttggcaaga atggttgaaa accacccggg agtagcattc tgaaatacca
    17461 gtaagaaatg acgccggctg cgttaaagct ttgagtgggt ttataccaat atgggtggaa
    17521 ccgcgggagt tagctctcgt ccctttcagg gacgggagct ttttgtattt tcaaatatcg
    17581 tagaggagca ggtgatagat atggtagaag taagagttcc ggctacaagc gcaaatatgg
    17641 gcccagggtt tgattgcctt ggagttgctt tgagtctgta taaccgtttt caaattgaag
    17701 aaagcggaaa cggattatta attgatggat gtgatgaaac ttacaggaac gaaaaaaacc
    17761 ttgtgtatgt ttcaatgcag aaatgctttg agaagatagg ctgcaataat agatacaagg
    17821 gattgagaat caacttcgaa agtgacatac ccatatcaag aggattggga agcagtgctg
    17881 catgcatact ggggggagtg ctggcagcca atgaaatagg cagaggaaac ctgaacaaga
    17941 atgagatatt ggagatcgct tcagagatag aagggcatcc ggacaatgtt gccccggcac
    18001 tgttcggagg gatgacagca tctgtaaaag acggaaacag ggtttattat gaaaaaatta
    18061 aacttcctcc ggggctgaaa ttctgtgcga taattcctga ttttaagctg tctacaaagg
    18121 aagcaagagc agtccttccg gacagtattc catacaagga cggcgtttac aatgtgggaa
    18181 gagtatccct gcttatagca gccctttcaa acggaaactt tgagatgttg aagctggcgt
    18241 gcaaggatag gcttcatgag atatacaggg gcaggcttat cggtaattac aacgaaattg
    18301 ttgaagaatg caacaggctg aacagtcttt gtgtattcct cagcggagca ggccccacca
    18361 tcatggctgt tttaaaagag gaagagacgg atttttgcag acaaatggaa gaatatttat
    18421 caaagcttga atgcaaatgg ataataaaag aactgaagtc cgatcaaaat ggggcttgtg
    18481 ttgtaaacat ttaattagga ggattattat gagaatcata gttcaaaaat ttggaggcac
    18541 ctcggttgca tcaggagaaa gaagaaattt ggtggcagat aaggtaatat gtgcgataga
    18601 gcaaggatac agccccgttg tggtagtatc agcaatggga aggaaagggg atccctatgc
    18661 caccgatagc ctcttatcac tgataaatga taaggattgc agtgttgata aacgggaggc
    18721 cgatctgctg atgtcctgcg gcgaaatcat ttctgcagtt gttatggcag aggcttttgc
    18781 tgaaagagga tataaggcga gagtcctgac agggggaaat gcaggaatca taacagacga
    18841 caattttggc aatgccaatg ttgtaagtgt tgaagccgaa aaggtcttaa aggtgcttga
    18901 gcaggggttg atacctattg taacaggatt ccagggaatg acggaggagg gagacttcac
    18961 tacccttgga agagggggga gtgatgtgac gggagccctg ctggggaaag cgttaaatgc
    19021 ggaatatgtg gaaatctata ccgacgtaga cggtattatg actgctgatc caaatatcgt
    19081 gcccaatgca aagataatag acaggataga ctatagagag gtttttgagt tggcgtatca
    19141 aggagcgaag gttatccatc cccgtgctgt ggaatatgct ttggaagata atataccttt
    19201 gattgtaaaa aatactttct ccgatacacc cggtactgta attaccaatt tggtggaata
    19261 cgaagaaaac ctgtttacgg gagtcacaag catgacaaac aaggccatgg ttattgtaaa
    19321 tccgagtgaa gtatttgaag gcggggctga ggctattttc aaactgctgg agaggaaaaa
    19381 aataagcatt gatgttataa gtgaatccca gtatcagaag gtttttatcg tggacagcga
    19441 tgcagcatcc gaggttgagg atatcttgaa acagctgtgt gcagagtata gaatcataaa
    19501 taattgcagc aagatttttc ttatagggaa taaagcaagc ggcatggagg aggtaatgtc
    19561 aaagctggta aaaacattgg aggtcagcaa tgtaaacgta ttgcagacag caacttcgca
    19621 taaggacatt tgctgcctga ttggagaggc agacaataaa aaagccttga acgagttgca
    19681 tagggcattt ttctgtgccg gctgataata tacagtatag ttgaaaggaa tgaagcaatg
    19741 atgggaaagg ttagaatagc attattaggg cttggaactg taggccaagg agtatggagc
    19801 atattaaaca ccaataagga tgatattttg aggaaatgcg gctgtgaaat agaggttgca
    19861 aaaatacttg tgagagatcc tgaaaaaccc agaggggtca aagtgccgga agggataatc
    19921 actgccgatg ttaacgatat tttaaatgac agcagcataa aaatcgtgat tgagcttatg
    19981 ggcggaactg agccggcaaa agattacatg cttagggcaa tgaagtgcaa aaagcatgta
    20041 gtaactgcaa acaagctggt attggcaacg cagggagagg agctcttcaa gactgcagag
    20101 caggagagag tgctcttcta ttatgaagca agtgtgggag gcggtatccc gataatcaga
    20161 gaaatcaatg agagtctgac tgccaataga attgacaagc ttgtaggtat aattaacgga
    20221 acgaccaact atattctgac taaaatgacc cgggagggca tggatttcaa cgaagccttg
    20281 agggaagcgc aggagaaagg atatgcagaa gcagatccct cttctgatgt agacgggtat
    20341 gatgcggctt ataagcttgc tataatgtcc tcattgtcct ttggtacgaa gattgatttc
    20401 tcttccatat acagggaagg tataagcaat ataaaagccg tcgatattga ttacgcaaac
    20461 aggttcggct atgttataaa gctcctggca ataggcaagg aagctgacgg aaggcttgag
    20521 ctgagagtac atcctgcaat ggttccaaag gaccatccca ttgcaaatgt aaatgattcc
    20581 tttaatgcta ttttcataaa gggaaatgcg gtgggagatc tgatgctgta cggaagaggg
    20641 gcaggagacc ttcctacggg cagtgcggta gtgggggata ttatttcaat cctgaggagc
    20701 aatattgcgt tatctgcgct atactccttc aagaacggca gggctcaaaa agaagtcaaa
    20761 agcgcatacg agaatgtatc cgaatactat atcagactta atgtaaagga tcagcccggg
    20821 atactgggag aaattgccgc agtgctgggg aggcatgatg tcagcatagt gtcggttaca
    20881 caggatatca aggacgaaga taatgtatcc attgtattta ttactcatga agccctggag
    20941 ggaaatgttg ccgaatcaat taaggaaatc agtatgcttg acagggttaa caatttggag
    21001 aatttaataa gaatcgaaaa ttttaattag tcgctaataa ataaaacctt gattttttca
    21061 aggttttctt caaatggagc tggagatggg actcgaaccc acgacctgag cattacgaat
    21121 gctctgctct accgactgag ccactccagc atttattaac atttgtattc tatttcagat
    21181 tccgtttcgg cttctggggc atgtcaatca gaaactcggt aatcaaaaaa cctgcaatta
    21241 tcggcagaga aatatagaac cacaaatcag atttctttac aagcttcagt atgaatgctt
    21301 cgacaagaat aactccaccc catatgagag attttttcgt ggttttatcc atattgtacc
    21361 tcctgctgct attgatatga atcaaagcaa gccactttaa tattctatca ctattataac
    21421 aaaaatgtaa gtcaggtaca cattaaaatt aaacgctaac tacatttttt tattttgttg
    21481 aagtattaat atttctaatt caaattaaaa ccacataata aaaggaatta ttgggaataa
    21541 aatagtcaga tgcgttataa ttgatacatg ggctagaacc ggcaaaacca tatagaatgg
    21601 agtgataaaa ttgaaatata aatacaaagg ctccctgagc ctgatcatga ttgcggcatt
    21661 cattatttgc gcggccgccg gaatggtgtc ctgtgcccca gccccaactc caactccaac
    21721 tccgactcca accccggctc cggaacctga acccgcgccg gaggaagagt cattaatagg
    21781 cttttatccg ctgaaggagg gcaatatgtg ggaatatgaa ggggaaggaa tggaatatgc
    21841 ctcttttatt caaaaggtaa tattcagctc gggaaacagg catcaggtta ccattgacaa
    21901 tggaggcacc ataatcgcca atgtgctgga ggaaagcgag ggcagcattg tgaatattta
    21961 taggtcggga gaagaattca gcggcgggaa tttacttgat aagccaagca atgtaaatat
    22021 aataatgctg caaacaccgc tggagaaagg gaacttctgg ataagcgaag aaaaccacta
    22081 tgagataata aatacggaag ctgatgtagt tgtacctgca ggagaattca aaggctgtat
    22141 agcagttaaa gttactttca aggaccagac ttcaaacatg acatactatt ataagcgggg
    22201 aataggtatg gttcagtccg agttcaggac ggattcaggt gacctgatta cgtctaaatt
    22261 gaaaagatat gagcttaagt aagataaaga agactcctac attgaggggt cttcctcttt
    22321 aattactgct ttatctacgc tcttgatttt tgaaagctgt accagcagta ctccggaaag
    22381 tataatagct gtagcaagca ataccgtagg agtgaagggt tccttcagga tcagagtacc
    22441 gagcaagaca gccacaggcg ggtttatata tgcataagtt cccgcttttg cagcaggcca
    22501 ttttcccaat atataaatgt atgaggaata accaagcagg gagccgaaga caataagata
    22561 aatcaatgcg ccgattcctt ttaatgagat acttaccctg ggaagctctc ccaaggatat
    22621 gccgagaatc agcaaagcaa ttcccccggc cagcatttgt atgcctatat ttacaatcat
    22681 tgaaccttca ggtttaaagc ttttagaata tactgagccc attgcccaga gaaaggatgc
    22741 agccagaatc aatatggcgc cttttacatt tatactgccg acacctgagc ttgagagaac
    22801 cagaattaca actccggaga atccaatgag caagccaagc cagcctttga attctattct
    22861 gggacggccg ggtataaaaa gttctattaa agccatgaat aaaggagcgg tagcaacaag
    22921 aagcgcggca attccggagt ggacccattg ctctgcccaa acaaccaggc cattgcctcc
    22981 aagcagaagg aacagtccta cgatagactg gtttttgata ctgttcagat ttgtggggaa
    23041 ctctaaaccc cgcaggcggg caaaaatcag cattagcaaa cctgcaacaa aaaaccttat
    23101 tccggcaaaa agctccggag gaaatacact gacaccgatt cggattgcaa gatatgtaga
    23161 tccccatatt atgcaaactg caataaacgc tgacataacc tttgattctt catttttaaa
    23221 aaacatatgt aagacctccc aggcactttt agaaccatta taaggcatgg gacagataat
    23281 gtaaatgaaa atgataatag cgattgacac atgaggagaa tggagctatc atatacatga
    23341 gcattaattg aatattgtcc gctaggggag cttagagctg agaaagcact ttgcggtttg
    23401 aatacgcata ggtgttgacc ctttgaacct gtctggttaa ttctaacgga gggaagcggt
    23461 tgtgattaat aaatagtctg accgtaactt ttggttacgg tctttttatt tagttattcc
    23521 tcccgggcaa taataaaaag aggtggtgta aaggtctctt attccggtaa tcaaaatttg
    23581 gcggtgattt atctgattaa agtcatctat tgggaggtaa tattatgaaa agggcatcta
    23641 cacaaatgct tgcagaggcg ggagttatga ttgcggctgc ccagatactc agctatgtaa
    23701 aaatatttga ggccccttac ggaggttcgg ttacggcagg gagcatgatt ccaattattg
    23761 tgttttcgct gagatgggga gtcaagtacg gacttcttgc aggcactgcc tatggaattt
    23821 tgcagttcct gctgggaggt gcaatttatt cctatcatgt aatatcaata ctttttgact
    23881 atgtagttgc cttcggcatg cttggactgg cagggttatt ccgcagcaac tttaaagggg
    23941 tatttaccgg tacatgcctg ggcgtggctg gaaggttcat ctgtcatgtg atatcaggag
    24001 tggtcgtatg ggcttcatat gctcccgaag gtatgaatcc gatgttttat tcaatagttt
    24061 acaacggctc ctatcttttg ccggagctta taatcactct aattattgta ggaatactct
    24121 ataagccgct gataagcaat ctgggcaaga gtgcataaaa aagcaatg
//
LOCUS       DAOOAB010000038        23713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2790_length_23713_cov_13.707583, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000038 DAOOAB010000000
VERSION     DAOOAB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23713
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_23713_cov_13.707583"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..510)
                     /gene="cas2"
                     /locus_tag="PKU88_08860"
     CDS             complement(220..510)
                     /gene="cas2"
                     /locus_tag="PKU88_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPX69422.1"
                     /translation="MMILITYDVSLEDTGGAKRLRGVAKACENLGQRVQKSVFECILE
                     PDQYIRLKNKLLSVIDQERDSIRIYHLGKNWERRVEHFGLTPRFEQEGTIIL"
     gene            complement(526..1554)
                     /gene="cas1c"
                     /locus_tag="PKU88_08865"
     CDS             complement(526..1554)
                     /gene="cas1c"
                     /locus_tag="PKU88_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HPX69423.1"
                     /translation="MKKLLNVLYVMTDNSYISKQGETVCVHVGGEEKVRVPAHTIESI
                     VCFGNTTVSTPLLSFCGEKGIGLAFFSDYGKFYGRLEGPVRGNVLLRRAQYKAAEKDD
                     VQFNMVKWFIAAKIANCRNILMRAAREGISEEASKVLTRAAGELSNIASQMGSKDCVD
                     ELRGLEGIAASKYFSVFDNMIRVNKDEFFFHSRSKRPPLDNMNCILSFLYALLVHDIR
                     SALEGVGLDPASGFLHTLRPGRPSLALDIMEEFRAPLCDRLALALVNLRQLSERDFEK
                     SVTGVILTDTAKRVILTAWQKRKKEEIKHTYLNEKVQIGLLPHIQALLLARYLRGDID
                     AYPPFYWK"
     gene            complement(1551..2219)
                     /gene="cas4"
                     /locus_tag="PKU88_08870"
     CDS             complement(1551..2219)
                     /gene="cas4"
                     /locus_tag="PKU88_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPX69424.1"
                     /translation="MSYSEEDLIPISYLSQYYYCHRRAGLLLLEEQWNDNVHTAEGSV
                     MHERVHAGGDESRGDIIITRSLYLRSFELGLSGIADVIEFHASSEGFEFPWMKGRWCA
                     YPVEYKHGEKRDELEYEVQLCAQAICLEEMANCKVQFGYIYYGSDRRRKKVFLNDDLR
                     SKVQKGAESLHIMMKSKVTPHEIKSPKCRECSMVDICLPGKLKSTRNYLDMLMHQIYG
                     DDDV"
     gene            complement(2216..3226)
                     /gene="cas7c"
                     /locus_tag="PKU88_08875"
     CDS             complement(2216..3226)
                     /gene="cas7c"
                     /locus_tag="PKU88_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HPX69425.1"
                     /translation="MIMSIKNRYEFLYFVECINGNPNGDPDSGNFPRIDPQDMHGLIS
                     DVAVKRRIRNYIQISKGNVSPYGIVVQQSTNINKFIAQAHEQAKNDSKAGTKDKVGAA
                     RRWLCENYYDVRTFGGVLSTGANAGQVRGPIQLAFPRSIDPVVPMDISITRMAVAKNI
                     KDVNTFEQYEKWESEQPEDELRTMGRKQLIPYGLYEGRGFISANLSEDTGFSEEDLIL
                     FWEAILNMYEHDHSASKGQMSTLHPLIIFRHVGTDSDEEQRERQAKLGCAPAHKLFEL
                     VKIRKKPEVSVARSYKDYVLKVSMSKAPKGVEIGFAIPAYNGVKIIWNNIPEELDWVT
                     AE"
     gene            complement(3234..4979)
                     /gene="cas8c"
                     /locus_tag="PKU88_08880"
     CDS             complement(3234..4979)
                     /gene="cas8c"
                     /locus_tag="PKU88_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HPX69426.1"
                     /translation="MLNELASYAKKYEIPTMPGFKAKKAQWVIQLSEKGKFIDILEDE
                     RTYVVCPHLEQNELIAGGISRSHFLLDTLSIVLDIDTDRKGRLKHEFFIKLLDEASEY
                     EPLLKHCADFLRDEFSLNSARQRVKTLKAKATNYVTFRIGSINVVDLVSWHDWWNNFR
                     SSIREKENNPPMLCLLSGNVVEPVSNHFKITGLTSVGGQASGTVLIGFDKDSYTSYGL
                     TQSLNAACSEESAALYRNALDNLIEKAPRPLAGTMFLHWYKEPIEDKDDILLFEGIDM
                     DEIEETSSRIRVEKLLRAIHEGERPELIRNKYYILQLSASGGRIMVRDWVCGDFSDLV
                     TKINKWFDDLSIVNPDGKGLAGDFKLSAAFLRMVDYRPGEEIRKTFARIDHELSPIFP
                     RMWRSILEGSPLMSLIADKALHYIKSKLFNSENDTNLDRVACALLKAWLIREEERRGG
                     KSSMQTGLNENHTSSAYHAGRMMAVLGDLQQCALGDVGSGVVQRYYAAVSATPALVLG
                     RLIRMAQFHLNKIESKGLANWYEKLMASIAEKMGDSIPTTMTLEEQALFALGYYQQKA
                     AMMAGKQNTNTNSNS"
     gene            complement(4984..5694)
                     /gene="cas5c"
                     /locus_tag="PKU88_08885"
     CDS             complement(4984..5694)
                     /gene="cas5c"
                     /locus_tag="PKU88_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HPX69427.1"
                     /translation="MRDIVIEAWGDLACFTRPESKVERFSYPVITPSAARGLLDAIYA
                     KPVEFCWRIDRIEVLNPIRFISLRRNEVKEKISENVVLKAIKGGDGPLIIADATKEMV
                     GNDKEGRTQRQTMALKDVRYRIFAHIEPRPEFEAKLKGFEEQALRRIEGGKCFYQPFF
                     GCREFVAYFEPSQMQKLPISESIDIGWMVYDVFDLDKIIIDEAQPYISLFRAELVEGV
                     MKIPDWRSEDVKKPNTRR"
     gene            complement(5709..7973)
                     /gene="cas3"
                     /locus_tag="PKU88_08890"
     CDS             complement(5709..7973)
                     /gene="cas3"
                     /locus_tag="PKU88_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPX69428.1"
                     /translation="MEYYAHTEDKRGIWEPVIKHLNEVAQLSKVFSSAWNAEIEGETV
                     GLMHDLGKYSELFQKVLKREEYHVDHATPGALAALKKYKENGMAVAMAIQGHHDGLQR
                     GNHRDFQTSVQMRTEVSKLGKKYSCRNDSQLLDRFYADGGSLIESFKSDFPEEYINKR
                     YISAMLYVRMLFSALVDADFLATEAHFSRIGENLIFRKQGVSLNPRIALDKLFDYKNY
                     LEKNSNANISINTLRNELFNSCISAGGNAKGLYTLTAPTGSGKTLAMLAFALKHALTH
                     KLRRIIIVLPFLNIIEQSAKVYNDILGSLSDGDFVLEDHSLVEWPEGEQMRLLAENWD
                     APVVVTTTVRFFESLFSNRPSACRRLHNISKSVIMFDEAQTIPPSLVIPTLSALSEIC
                     WRYGSTVVFSTATQPAYEFFDDNVRKYSIKGWKPSEIVTDRLNLFGKSKRVEVNWKKE
                     RYTWDKLADAINKDTQSLTIVNLRRHAKELYKKVYEEANLYFGKEASNIIFHLSTDMC
                     PTHRLDVLNKVKQRLKDDMPCYLISTQCVEAGVDIDLPVVWRALAPLEAIIQAAGRCN
                     RNGRKKAGIVHIFLPSIEEELYPTNDYHYGAIVVKQMLEEGDIDFYDTDTIRKYYQRY
                     FEIARLSQKEDKLQRAITAVDFPEVARYYRWIPPIATNVLVPYQPEIEVFEALCKEAR
                     KGDITREWLSKARRLSVGVILNKNSNISDYLEEVRDKRGDPTGWYILVNSKAYTINTG
                     IDISEAKPEEFYVT"
     gene            complement(8385..9191)
                     /locus_tag="PKU88_08895"
     CDS             complement(8385..9191)
                     /locus_tag="PKU88_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69429.1"
                     /translation="MIIKLLEQPIVAIHEAEITGTVDGVIIKGSKVNCIYHGNMEDHF
                     VIPIERAIIGSDAVMIPDVALMTMASKNVKPLRSMINVYNLKGKRLGYLQGIEVDEQF
                     VARYIYTEEYRIEMSKAVSYDSVLIVDIEEADLEAIEEKQDVLEDTENEEAEVAGASG
                     GNSAESTEAAVSMEIGPEINWEAEDYKPEEKSAESSELSLVKPVHHEEDKKEIPYVEA
                     KYAYLCGKQLLEGIDIDDTFYKKGTIIDADLIKHAISSNAIVKVIVNAEE"
     gene            complement(9203..10342)
                     /locus_tag="PKU88_08900"
     CDS             complement(9203..10342)
                     /locus_tag="PKU88_08900"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HPX69430.1"
                     /translation="MESRVKIYADGGNRLTKIMEESRKPFDFPTIISEPEKELNYGLN
                     FDFDLSDWNNDAAKLDINNMFVEIIKDGVSMGKMLVGKAAEEKGLLIRDRNRYQEKAA
                     DDVIMFCTIAGIAYNLAKYNKNYQKIDLTVNQPLIEYARNKEASANKYTENLMGNMKV
                     LFFNIFNPSQVIYEVIFDIEKVVLCPEGIAAFFQYAVNEDGSLKEEFKEDMKTIVWDI
                     GSGQINVAAFDGFKVAGVNTFEKGMFDCYEKISLILYNNFRDKLDRKPYSYEVDNMIR
                     FNNSVLKGKKGEDIDFNNIVKNVFDVFAYDLSKDIREFSKIKFMGACDSVIFAGGGSK
                     ILFDYLSKYLSNDYICVKSNIGEYDNVVGSMYYRMYKDVRSSIEG"
     gene            10517..11827
                     /locus_tag="PKU88_08905"
     CDS             10517..11827
                     /locus_tag="PKU88_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69431.1"
                     /translation="MENVSNKNALKVHRCIISTGSSSNSYLTRYASDHVEESISICNC
                     LDKEDIPKITPASHYDIIISYSDETIEEIIYDLFSYLIPQDVTIRAAYILDNSMSGSS
                     MPKHVAFLEKLEKHKFITLEFIDDSPEEPVEQEENIIYFVGPGNTGKTSIIASLSESF
                     RQNGQKLALIDITRNNKLINYFPKHSFLNTGNLKDFSISRDFYRSVNIYTDGLVDLYV
                     YDYEYDKKGLEVIYFCEILRKLSGLYDYIIVNADESSVINTPYIYKTASKVFIVHDFM
                     PTKIRTTKKLLLKMISAGINTQKTVSLIYNKTLKDTIDIGRIEEKLLFIRLPNRKILP
                     TVNINCSTFEIPYEQRTMAALVNNLAQNTTGIENTSKKYRHIIQNIYKYINNIPYTEN
                     DEMEISQFLREKLRKLSDRKFMLEYMRNICDTSLIKNYIKAYKK"
     gene            complement(11850..13094)
                     /locus_tag="PKU88_08910"
     CDS             complement(11850..13094)
                     /locus_tag="PKU88_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX69432.1"
                     /translation="MDSRNHSLADFFDVDADMAGRAKMFGEYLKDIREKRHLQYRRIS
                     MDGSGPVMKVVDHYTGKIKEMVYLASNDYLNLTKHPRTIAAGRKALEKYGSGAGSVPL
                     LGGTLDIHIELEKKIAKFKGCEDSIIYTSGFGSNCGSLLSILQEKDIAILDTYVHASI
                     IDGCKSTNIRFFRHNDMNSLERVLQRVKDKYRTKLIAVDGVYSMDGDIASLDRIAELA
                     KTHGAYVMVDEAHATGVIGDTGRGTPQHHKIEGKVDIVAGTLSKALGGVGGFIAANSE
                     LVELLRYYSRPYIFSTAMTPQVAGSLLAAIDVIEEEPEHRERLWENIRYFRSNLTALG
                     FDLGNSETAIFPIIIGNDLKVKEICRRLHELDIYANPVMYPAVPKRLSRIRMSLMSAH
                     TKEHLDKALDALETAGREYGVI"
     gene            13291..14583
                     /gene="nagZ"
                     /locus_tag="PKU88_08915"
     CDS             13291..14583
                     /gene="nagZ"
                     /locus_tag="PKU88_08915"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPX69433.1"
                     /translation="MKRLFSIVIVVLILLIAASCTVPDVSEGNTPPVPPAQSDPEAED
                     RKDPLEAKAESLLAGMTIEEKAGQLLIVGFPGDTKKEALQDYIDRLKVSGFILFSRNY
                     TDFDSLYALARSLKEMNSLNNPLPLFISIDEEGGTVSRLPKGGTRFPDARKVGKAGEP
                     GLTYKAGQTIAKELKAAGINLNFAPVLDIVENGENKLLIKRSYGSTPEVVSLHGTSFI
                     SGLQSEGVTAVPKHFPGHGNTNQDSHSTLPVIDTDKAAMQSRELVPFKAAIDAGLDAV
                     MVGHIAFPKLDPTGLPASMSSYFLTDVLRKDLGFGGISISDDIEMQGYISSKDTVEEC
                     VISSFNAGLDIFLIGHTKAIQDQVYKALLDGCRDGRISEERLNESVLRIIKAKLKNGL
                     TDAMEYEIEEAKRIFGSDEHKAILEELNGDTGTFLLSR"
     gene            complement(14641..16500)
                     /locus_tag="PKU88_08920"
     CDS             complement(14641..16500)
                     /locus_tag="PKU88_08920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPX69434.1"
                     /translation="MNADVFLYFIFITAKLLFFNSIIDGTGMGSIPLIASVAGTVLLL
                     FFWAGLLPGKIRLPLLFMLDCAVTFIIMADLLFFRYFSDVLSMPVLEQASVVTSVKSS
                     ILNLLHASDLIFAADLLFLPFVSAKAGRLNKKPKAGFGKRIICALSALAIGIGLCGCG
                     VSEILKAQPEIFRSFYDRVYIVQNIGLLGFHGIDAFRYVKSKDKLELSLSEDIKKEIA
                     EFIKDKKPEQPSSSKLFGTGKGKNLIVVQVEALQEFVIGKSIEGQEITPNLNKLIKSS
                     LYFDNYYTQTAGGGTSDAELLSNVSMHPARDGATYIRFPLNDYYSLARRLSEEGYLTS
                     AMHAYRPGYWNRSVMYKTLGFDEFIDKEDYIPDEISGMGLTDKSFFRQSLEFMEKYKE
                     PYYTFLITLTSHFPYDNDKKYLGSFNAGKYKGTFFGSYLEVIHYADEALGGFIDELEA
                     RGILDRSVLAVYGDHFGIPRDKKEYLADFLGLKEMDDYNWVKQQRIPLIIRIPEGKYT
                     GTKHIAGGGLDFMPTILNIMGVDSSNIPMLGRDLLNSEEGMAVLRHGYFITDKYICLT
                     SKGVAYDAETGSTYPAEKLAKEKEFAARQLRYSDLITEYNLVKELMEYLKDET"
     gene            complement(16748..17992)
                     /locus_tag="PKU88_08925"
     CDS             complement(16748..17992)
                     /locus_tag="PKU88_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPX69435.1"
                     /translation="MATVKAFRAIRPAPELAHKVAALPYDVMDSNEARGMVKGNPYSF
                     LHVDKAEIDLDPSIDLYDKRVYEKARDNLREMIKNGVFQQDEKNCLYIYKQVMKGRVQ
                     TGLVGCTSIDDYMNNIIKKHEYTRADKEQDRINHVDYCNANTGPIFLTYRASKEINEI
                     VDNWMADHKPVYDFVSDDGIGHTVWVIDGDDAIQRLEERFRAVDYLYIADGHHRSASA
                     VKVGLKRRESFPDYKGDEEFNFFLSVLFPDEQLYIMDYNRVVKDLNGNSAEEFLKKVG
                     EKFDIEEYTGEGQFRPTVRHTFGMYMDGKWYKLTAKAGTYNEEDPVDRLDVSIMQNNL
                     LDPVLGIKDPRTDKRIDFVGGIRGLGELERRVNAGMKVAFSMCPTTIMDLMSIADAGK
                     VMPPKSTWFEPKLRSGLFIHEL"
     gene            complement(18004..18915)
                     /locus_tag="PKU88_08930"
     CDS             complement(18004..18915)
                     /locus_tag="PKU88_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010299674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPX69436.1"
                     /translation="MKRILVTDGMEKSAVEKLKNSGYEVVEQFYEPEVLKEQIKNFDA
                     VVVRSATKVRQPIIDAALETGRLKLIIRGGVGVDNIDVDYAKLNGIAVANTPNASSAS
                     VAELAIGHMFCLARHIYIANHTMRQGKWNKKQYEGIELSGKTLGLVGFGRIAREVAKR
                     AFALGMKVVYTNTSGAKEGFNDYKYMKLDELLAVSDFISMHIPCSQDQQPVIGEAQFA
                     KMKDGVYLVNTARGGVVCEEALLKALDSGKVAAAALDVFEEEPTKNERIYTHERISLT
                     PHIGASTNEAQERIGGEIIQIIQNFFE"
     gene            complement(18960..20036)
                     /locus_tag="PKU88_08935"
     CDS             complement(18960..20036)
                     /locus_tag="PKU88_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPX69437.1"
                     /translation="MHKKLFIPGPIDVREDVLQKMATPQIGHRTKEASALQRSVSEKL
                     MKVFQTKNQIILSTSSGSGLMEGAIRSCTRKRAAVFSVGAFGDRWFKMAKANGVPADK
                     ISSVPGKPTTPEMVDEALATGKYDVITVTQNETSAGIMNPCGEIGELLKKKYPDVLYL
                     IDTVSSMGGTDIRVDEWGADVCITSTQKCLGLPAGMSACSISERAVEAARQVENRGMY
                     FDLVELYDFIQKKDHQYPSTPSLAHMFAMDYQLDKILAEGLEKRFARHIEMAEYVRAW
                     ARKNFALFVEDEKYLSNTLTTVTNTIGIDVGGLNKKLGERGFMISNGYGDLKDKTFRI
                     SHMGDYTLGEVKELIKNINEILGL"
     gene            20275..20880
                     /locus_tag="PKU88_08940"
     CDS             20275..20880
                     /locus_tag="PKU88_08940"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPX69438.1"
                     /translation="MEMDFTKLVNRKTAICDKEVYLEFSVLVPIIKTGSEFSLLFEVR
                     SEKLAKQPNEICFPGGKIESFENARQAALRETTEELLIPEANIDVIAELDTIVTPFNR
                     ILYPFAGKIHEYNGTFNSEEVKETFIVPLSFFLENAPLHHDINISMKPVEGFPYHMVR
                     DGRDYPWGKGKYPVYFYTYENKIIWGLTARIIKNFVNLLGS"
     gene            20918..>21276
                     /locus_tag="PKU88_08945"
     CDS             20918..>21276
                     /locus_tag="PKU88_08945"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPX69439.1"
                     /translation="MEIKRIKGVIPPMITPFKENGDVDYRMHAGNMEKWNNDNLAGCL
                     VLGSNSETVYLNEEEKLELIRITVEAARKDRLILAGTGMESTRETIKLTNKAARLGAH
                     GALVLTPFYYGGKMNDEA"
     assembly_gap    21277..21376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21377..21605
                     /locus_tag="PKU88_08950"
     CDS             <21377..21605
                     /locus_tag="PKU88_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPX69440.1"
                     /translation="VQEYYENGQIEKAKDTYTRIFPVNTAVTATYGIAGLKYACSLSG
                     YEGGYVRKPLLELKEDEKKKVRDILSKAGLI"
     gene            complement(21656..23701)
                     /locus_tag="PKU88_08955"
     CDS             complement(21656..23701)
                     /locus_tag="PKU88_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPX69441.1"
                     /translation="MNKDKAANRLITEKSPYLLQHAYNPVDWYPWGQEAFDKAKAEDK
                     PVFLSLGYSTCHWCHVMERESFEDKEVAEILNKNYIAIKVDREERPDIDHIYMNVCQA
                     LTGSGGWPLTIIMTPEKAPFFAGTYFPKRARMGYPGVIELLEKINEAWNTEREKLLEA
                     SENILEHIRSDSAADSAVELDIETAGKAFKGLERSFDSEYGGFGRAPKFPTPHNLRFL
                     LRYWKVTNDPKALQMVEKTLISMYRGGIFDHVGYGFSRYSTDRIWLVPHFEKMLYDNA
                     GLAAAYLEAYQATGKEQYGEVARQIFEYVLRDMTSPEGAFYSAEDADSEGVEGKYYVW
                     SPKEITNILGEDAAVEFNGYYGLSDDGNFEGKNIPNLLHNKAAFENIRKNPLEGHREK
                     VFEAREKRIHPYKDDKVLTAWNGLMIAALSIGARVLDNMEYAVAAERAVRFIGSKLIR
                     EDGRLLARYREGEAAYPGYLDDYAFLVWGLIELYEATFRAEYLKLAADLNRDMLKYFY
                     DDEKGGLFLYGSDSEQLIVRPKDVYDGAMPSGNSVAAMNFLRLGRLTGDTDLEDKAYK
                     QLEAFGSIIKGHPMGYADMLSALLFINSKASEIVIVGNREDENAKRMIKSINSHFSPF
                     TVVLFKDKENAEEGITSIAPYTEAQGMVDNKTTAYVCENFACRAPITDLQEFENVLA"
BASE COUNT         6418 a         5636 c         4268 g         7291 t
ORIGIN      
        1 atgtttcaat ccacgcccct gcgcgagggg cgacagcgca cactgtaaaa ctttatggtt
       61 ctagcattat atgcatacta ttcgcatacc gcaaataatc tgtatatact tagtctctaa
      121 agcagtacaa aatccagttt attctcatca taagtgagta cttacaagcc tgcgcgaatt
      181 tttatggaaa tatatgttca tttaaagtac gcgcagatac tagagtataa tcgtaccttc
      241 ttgttcaaat cttggggtaa gaccaaaatg ttctaccctg cgctcccaat tcttccctaa
      301 atgatatatt cttattgaat ctctttcttg atcaattact gaaagtaatt tatttttcaa
      361 tcttatgtac tgatctggtt ctaaaataca ttcaaataca gatttttgca ctctctgacc
      421 aagattttca catgctttgg caacacctct tagtcttttt gctccccctg tatcctctaa
      481 gctaacatca taagttatta atatcatcat ttccaatgcc actccttact tccaatagaa
      541 aggaggataa gcatcgatat cacctcttaa gtaacgcgcc aagagtagag cctgaatatg
      601 cggtaataaa cctatttgta ccttttcatt taagtacgta tgcttaatct cctccttttt
      661 tcttttctgc catgctgtta gaattactct tttagcagta tcagtcaata ttactccagt
      721 tactgacttc tcaaaatccc tttctgaaag ctgtctaagg tttacaagag ccaaagcaag
      781 cctgtcacat aaaggtgccc taaactcttc cataatatct aaagccaagc taggtctacc
      841 tggccttaat gtatgaagaa atcctgaagc tggatcaagt ccgacacctt ccaaagcaga
      901 acgaatatcg tgaaccaaca aagcatacaa aaatgaaagt atgcaattca tgttgtcaag
      961 cggtggtcgt ttgctgcgac tatgaaaaaa gaactcatcc ttattaactc gaatcatatt
     1021 atcaaacaca ctaaaatatt tactcgctgc aattccttcc aaacctctca actcatcgac
     1081 acaatcctta ctccccattt gactggcaat gtttgaaagt tcgcctgcag cacgagtcaa
     1141 tactttgctt gcctcttcac taataccttc tcgggcagcg cgcataagaa tattacggca
     1201 attagcaatt ttagccgcaa taaaccattt aaccatattg aattgaacat cgtccttttc
     1261 tgcagccttg tattgtgctc tcctcagaag aacatttcct cttaccggtc cctcaagcct
     1321 tccatagaat tttccataat cactgaaaaa ggctaaccca atacccttct caccgcaaaa
     1381 agataatagt ggtgtcgata cagttgtatt tccaaagcac actattgatt ctatggtatg
     1441 tgcgggtact cgtactttct cctctccccc gacatgtaca caaacagttt ctccctgctt
     1501 ggatatgtat gaattgtcag tcatcacata aagcacattc aagagttttt tcatacatca
     1561 tcatctccat atatttgatg cattagcata tccagataat tacgtgtact tttgagtttc
     1621 cctggcaaac atatatctac catagagcat tctctacatt ttgggctttt aatttcatgg
     1681 ggagtaactt ttgacttcat cattatatga aggctttctg cccctttctg taccttgctt
     1741 ctaagatcgt cattcagaaa tactttcttg cgcctccggt cagatccgta atatatgtaa
     1801 ccaaattgaa ctttacaatt agccatttct tcaagacaaa ttgcttgtgc acagagctgc
     1861 acttcgtatt caagttcatc tcttttttca ccatgtttat attcaacagg atatgcacac
     1921 catcttcctt tcatccaagg aaattcaaac ccttcgctag aagcgtgaaa ctctataaca
     1981 tcagcaatac ctgagagccc gagctcaaaa gaacgaagat ataagctcct tgttataatt
     2041 atgtcgcctc ttgattcatc tcccccagca tgcactctct catgcataac tgagccttct
     2101 gctgtatgga cattgtcatt ccactgttcc tctaaaagca agaggcctgc gcgacgatga
     2161 cagtaatagt attgagaaag atatgaaatc ggaattaaat cctcttccga ataactcatt
     2221 ccgctgtcac ccaatccaat tcttcaggta tattgttcca tataattttc actccattat
     2281 atgcaggtat tgcaaagcca atttcaacac ccttaggtgc tttactcata gaaactttca
     2341 atacataatc tttataactt cttgcaactg atacttcagg cttttttcga atctttacca
     2401 attcaaaaag tttatgtgca ggtgcgcacc ccaattttgc ctgcctttcc cgttgctctt
     2461 catccgaatc ggtacctaca tggcggaaaa taattaaagg atgtaaagtt gacatttgcc
     2521 ccttgctggc cgaatggtca tgctcataca tgttgagaat ggcttcccag aataatatca
     2581 gatcttcttc cgaaaaacct gtgtcttcag aaagatttgc actaataaat ccccgtcctt
     2641 cataaaggcc ataaggtata agctgcttcc ttcccatagt gcgcaattca tcctctggtt
     2701 gctcactttc ccatttttca tattgttcaa aagtatttac atcctttata ttctttgcaa
     2761 cagccattct agttatcgaa atatccatag gcactacagg atcgatggaa cgcggaaaag
     2821 ctaattgaat aggtcctcga acctgacctg catttgctcc tgttgaaagt acacctccaa
     2881 aagttcttac atcgtaataa ttctcgcaaa gccatcttct tgccgctcct accttgtctt
     2941 tcgtaccagc tttgctatca ttctttgctt gttcatgtgc ctgggcaata aacttattta
     3001 tattcgtaga ttgctgcaca acaataccat agggtgaaac atttcctttt gatatttgta
     3061 tataattcct aatcctcctc ttaactgcca catcggaaat aagtccatgc atatcctgag
     3121 gatctatgcg cggaaaatta ccagaatcag gatccccgtt tggattacca ttaatacact
     3181 caacaaaata aaggaattca taacgattct taattgacat gattatcctc ctctcaagaa
     3241 ttggaatttg tattagtatt ttgttttcct gccatcatgg cagctttttg ctgatagtat
     3301 cccagtgcaa atagtgcctg ctcctccaac gtcattgttg tcggtatgct atcacccatc
     3361 ttttctgcaa tagaagccat tagtttttca taccaatttg ctaatccctt gctctcaatc
     3421 ttgtttaaat gaaattgagc cattcttata agccttccaa gaacaagtgc cggagttgca
     3481 cttacagcag cataataccg ctgaacaaca cctgatccta catcaccaag tgcacattgc
     3541 tgcaagtcac cgagcactgc catcattctt cctgcatgat aagcagaaga tgtatgattt
     3601 tcattcagcc ccgtttgcat actgctctta cctcctctcc tttcctcttc ccggataagc
     3661 caggctttta gaagtgcaca ggcaacccgg tccagatttg tatcattttc tgaattaaaa
     3721 agttttgact ttatataatg caatgcttta tctgctatca gggacattaa tggacttcct
     3781 tccaatatgc tgcgccacat tcttgggaaa ataggtgata attcatgatc aatacgagca
     3841 aaagtttttc ttatttcctc ccctggtcta taatcgacca ttcgtaaaaa ggctgcagat
     3901 agtttaaaat ctccggctaa gcctttaccg tctgggttta caatggataa gtcatcaaac
     3961 catttgttta tctttgttac aaggtcagaa aaatctccgc aaacccaatc acgtaccatg
     4021 atacgaccac ccgatgcaga caattggagg atgtaatatt tattcctaat cagttcaggt
     4081 ctttctcctt catgtattgc tcttaataat ttttcaactc ttatccttga actggtctcc
     4141 tcaatttcat ccatatcaat tccttcaaat aataaaatat catccttatc ctcgataggt
     4201 tctttatacc aatgcaaaaa catagtacct gccaacggtc taggtgcttt ctcgatgaga
     4261 ttgtcaaggg catttctata taatgctgca gactcttcag aacatgctgc attcaatgac
     4321 tgtgtaagtc cataggaagt ataagaatct ttatcaaacc ctataagtac agtaccactg
     4381 gcttgtcccc caactgaagt caatccagtt attttgaaat ggttcgatac aggttcaact
     4441 acattgccgc taagcaggca caacataggt gggttatttt ccttctctct tatggaagat
     4501 ctaaaattat tccaccaatc atgccagctt acaagatcca caacattaat acttccaata
     4561 cggaaggtta catagttcgt agcttttgct tttagtgtct ttactctttg ccttgcgctg
     4621 ttaagtgaaa actcatccct cagaaagtct gcacaatgct ttaatagcgg ttcatattca
     4681 gaagcttcat ctaaaagctt aataaaaaat tcatgcttta atcttccctt tctgtcagta
     4741 tcaatatcca atacaattga caaagtatca agcagaaaat ggcttcgaga aatgccgcct
     4801 gcaataagct cattctgctc aagatgtggg cataccacat aagtgcgctc atcctccaat
     4861 atatcaataa actttccttt ttcacttagt tgaattaccc attgagcctt ttttgcctta
     4921 aaccctggca ttgtcggtat ttcatatttc ttagcataac ttgcaagttc attaagcaca
     4981 taatcacctc cttgtatttg gtttttttac atcttcactt ctccaatcgg gtattttcat
     5041 aacaccttct actagttctg ccctaaaaag acttatataa ggttgagctt cgtcaattat
     5101 tatcttgtcc aagtcaaaaa catcatagac catccatcca atatcaatac tttcacttat
     5161 agggagtttc tgcatttggg atggctcaaa gtaagcaaca aactccctgc agccaaaaaa
     5221 gggctgatag aagcacttcc ctccttctat acgtctcagc gcttgttcct caaatccttt
     5281 tagttttgct tcaaattccg gcctcggttc gatatgagca aatattctat atcgtacatc
     5341 ctttaatgcc attgtctgcc tttgagttct accttcttta tcatttccga ccatttcctt
     5401 tgttgcatct gcaataataa gaggcccatc tcctccttta atcgccttta gtacaacatt
     5461 ttcgctgatt ttttctttta cttcatttct cctgagagaa ataaatcgta ttggatttag
     5521 tacttcgatc ctgtcaattc tccagcaaaa ttccaccggt ttagcatata tcgcgtcaag
     5581 taaaccccgc gcagctgaag gggtaatcac agggtaagaa aatctttcaa cttttgactc
     5641 tggtcttgta aaacatgcca agtcacccca tgcttcaata acaatatctc tcatccttcc
     5701 tcacctcctt acgttacata gaattcttct ggttttgctt cagaaatatc aataccagtg
     5761 ttaattgtat atgctttgga attcaccaat atataccacc ccgtaggatc tccccttttg
     5821 tcccttacct cttccaagta gtctgaaata ttactattct tgtttaatat aactcctaca
     5881 gataatcttc ttgccttgct aagccattcc cttgtaatat ctcctttcct tgcttctttg
     5941 cacaatgctt caaatacttc aatctcaggc tgataaggta ctaatacatt tgtcgcaata
     6001 ggaggtatcc accggtaata tcgtgcaacc tctggaaaat caacagctgt tatagccctt
     6061 tggagtttat cttccttttg actaagacga gctatttcaa aatatctttg atagtatttc
     6121 cttatggtat ctgtatcata aaaatcaata tcaccttctt cgagcatttg ttttactaca
     6181 atagccccat aatgatagtc atttgtagga taaagttctt cttcaatcga cggtaaaaaa
     6241 atatgcacta tgcccgcctt ttttcttcca tttctattac atcgccctgc tgcttgtata
     6301 atagcctcta gtggagccaa agccctccaa acgactggta aatcaatatc aactcctgct
     6361 tctacacatt gtgttgaaat aagatagcag ggcatatcat ctttgagcct ttgctttact
     6421 ttattcaaga catcaagccg atgtgtaggg cacatatcgg tagacaaatg aaaaataatg
     6481 ttgcttgctt ctttaccaaa atacaaatta gcttcttcat ataccttttt atagagttcc
     6541 tttgcatgcc ttctgagatt tacaatagtt aaagattgcg tatccttatt aattgcatct
     6601 gcaagcttgt cccatgtata tctttctttt ttccaattaa cctctaccct ctttgacttt
     6661 ccaaataggt ttagtctatc agttactatt tcacttggtt tccagccttt tatagaatat
     6721 ttcctaacgt tatcgtcaaa gaactcataa gcaggctgtg tagctgtaga aaaaacaacc
     6781 gtgcttccat atctccaaca aatctctgat aaagctgaaa gagttggaat gactaaactc
     6841 ggaggtatcg tctgggcttc atcaaacatt attaccgatt tcgagatatt atgaagtctt
     6901 ctacatgctg aagggcgatt ggaaaaaaga ctttcgaaaa atctgactgt agttgttaca
     6961 acaactggag catcccagtt ttctgctaga agtctcattt gctcgccttc tggccattca
     7021 acaagactat gatcttcaag tacgaaatct ccatcagata aactgcctaa tatatcatta
     7081 tatactttag cagattgttc tattatattg agaaaaggaa gtacaataat aattcttctt
     7141 agtttatgtg ttagtgcatg ttttaacgca aatgccagca ttgcaagcgt tttccctgac
     7201 cctgtaggtg ctgttagtgt atataagcct ttggcatttc cacctgcact tatacaggaa
     7261 ttgaaaagtt cattacgaag tgtattgata ctaatatttg cattagaatt tttctctaaa
     7321 tagtttttat aatcaaaaag tttgtcaagt gcaatccgag ggttcaaact gacaccttgc
     7381 tttcgaaata ttagattctc acctatacga ctaaaatgag cctctgttgc tagaaaatct
     7441 gcgtctacta aagcagaaaa taacattctt acatataaca ttgctgaaat ataccgttta
     7501 tttatatact cctcaggaaa atcactttta aaactttcaa tcaaagagcc gccatcagca
     7561 tagaatcgat ctaataactg gctatcattt ctacaagaat atttcttacc tagtttcgaa
     7621 acttcagtcc tcatttgaac gcttgtctga aagtccctat gatttccccg ttgcaatcca
     7681 tcatggtgtc cctgaatagc cattgcaact gccattccgt tttctttata cttttttagt
     7741 gcagccaatg ctccgggagt tgcatgatcc acatgatatt cttctctttt taatactttt
     7801 tgaaataatt cactatattt gcccaaatca tgcatcaacc ccactgtttc tccttcaatt
     7861 tctgcattcc aagctgaaga aaacacttta gagagctgtg caacttcatt caagtgtttt
     7921 attacaggtt cccatattcc tcttttatct tctgtatgtg cataatattc catatagact
     7981 tttctcctca tatttacttt taattatata ctttccatta ctgtatatca atgtaatact
     8041 aaaaattgta tattatagaa taaaaagtct aaaaatggcg ctttcgctgt gtattttcag
     8101 acttccaaac atattgattg tattaattaa ttatcctaca ccttaccatc ccgctacaac
     8161 agctccatca atatattaat cgcataaaga ggtacattgg ttaaccagga ttcctttctg
     8221 taatcagaca ttaaggtgtg aaaagcctgc ttcggattat gtttaaacat aacaattcac
     8281 aaaaatcaaa ggacttttgt gaatatcata cattttttca aacgacttta tgtaatattt
     8341 gctgcaaact gcactaaaaa agaaggcgat ttctcgcctt cttcctattc ctctgcgttt
     8401 actattacct ttacgattgc attgctgctg attgcatgct ttatcaggtc agcatcgatt
     8461 attgttcctt tcttgtagaa ggtatcatca atatctatgc cttcaagaag ctgtttgccg
     8521 cacaggtatg catatttggc ctctacatag ggaatctctt ttttgtcctc ttcatggtgt
     8581 acgggcttta ccaggcttag ttccgagctt tcggcactct tctcctccgg cttgtagtcc
     8641 tcagcctccc aattgatttc cggccctatc tccatggata ctgctgcttc tgtactttct
     8701 gctgagttac ccccggaagc tccggcaacc tcagcttctt cattttcagt atcctccaga
     8761 acgtcttgct tttcttcaat tgcttcaagg tccgcttctt ctatgtctac gatgaggacg
     8821 ctgtcatagc tcactgcctt cgacatttca atcctgtatt cctctgtgta tatgtaccgg
     8881 gcaacaaatt gttcgtctac ctcaattccc tgcagatatc ccagacgctt tcccttgaga
     8941 ttgtatacat ttatcatcga cctgagaggc tttacattct ttgaagccat ggtcatgagc
     9001 gccacatcgg gaatcatgac ggcatcgctg ccgataattg ctctttcaat cggaatgaca
     9061 aaatgatcct ccatattgcc gtgatatatg cagttgacct tgctgccttt gattatcact
     9121 ccatcaaccg tacctgtgat ttccgcctca tgaattgcta caattggttg ttccaacagt
     9181 ttaataatca aaaagccggc acctaccctt caatacttga ccttacatcc ttgtacatcc
     9241 tgtaatacat gcttcctacc acgttgtcat actctccgat gttgctcttt acacaaatat
     9301 aatcattgct gagatacttg ctgagataat cgaaaagtat cttgctcccg ccgccggcaa
     9361 atattacact gtcacaggca cccataaact taattttgga aaattctctt atatccttgc
     9421 tgagatcata tgcaaaaaca tcaaatacat ttttaactat gttgttgaaa tcaatatctt
     9481 ctcccttttt acccttaagc accgagttgt tgaatcttat catgttgtct acttcgtaac
     9541 tgtatggctt tctgtccagc ttgtccctga agttattgta cagaatgagg gagatttttt
     9601 cgtagcagtc gaacatacct ttttcaaagg tgttgacccc cgctaccttg aagccgtcaa
     9661 atgcagctac atttatctgg ccggaaccaa tgtcccagac tattgtcttc atatcttcct
     9721 tgaattcttc cttcaggctt ccgtcttcat tgaccgcata ctggaagaat gctgctattc
     9781 cttcggggca caatacaacc ttttcaatgt cgaatattac ttcgtatatt acctgggaag
     9841 ggttgaatat attaaagaat aataccttca tgttgcccat aaggttctct gtatatttgt
     9901 ttgcagatgc ttctttattt cttgcatact cgataagagg ctggttgact gtaaggtcta
     9961 ttttctgata gttcttgttg tatttagcca gattatatgc aatgcccgca attgtgcaga
    10021 acattatcac atcatctgct gccttttcct ggtatctgtt tctatccctt attaaaagtc
    10081 ctttttcttc ggcggcctta ccgaccagca tcttgcccat tgatacgcca tctttgatga
    10141 tttcaacaaa catattgtta atatcgagtt ttgcggcatc gttattccag tctgaaaggt
    10201 cgaagtcaaa gttcaatcca taattcaatt ctttctccgg ctcagatata atagttggaa
    10261 aatcaaaagg ctttctggat tcctccataa ttttcgtcaa tcgatttcca ccatctgcat
    10321 aaattttaac tcgagactcc attaaaagac ctccttcaat attaaaaatg aatacaacta
    10381 ctctataata tacgatactc catatcttcc cagcatcata taggacttta gtcctaattt
    10441 gtctgattgt gttgaaaaaa tagccatttt gtgttaattt caaggtgtgt gcagctttat
    10501 caaagggggg gatagagtgg agaacgtaag taataaaaat gctttaaagg tgcatcgatg
    10561 catcatttcc acaggttcat cctctaattc ttaccttacc cgttatgcct cagatcatgt
    10621 cgaagaaagc atatccatct gcaattgttt ggataaggaa gacataccca agataacccc
    10681 ggccagtcat tatgatataa tcatcagtta ttccgatgaa acaatagaag aaatcatata
    10741 tgacctgttc agttatttga ttccccagga tgttaccatc agggctgcat atatattgga
    10801 caacagcatg tccggaagca gcatgccgaa gcatgtagca tttttggaaa agcttgaaaa
    10861 gcacaaattc attactcttg agtttattga tgacagtcct gaagagcccg tggaacagga
    10921 agaaaacata atatatttcg tagggcccgg caataccggc aaaacctcaa ttatagcgtc
    10981 cctcagcgaa agcttcaggc agaacggcca gaagctggca cttatagata taaccagaaa
    11041 taataagctt atcaactatt tcccaaagca tagctttctc aacaccggca atttgaagga
    11101 tttttccata tccagggatt tttacaggtc tgtgaacatt tataccgacg ggcttgtaga
    11161 tttgtatgta tatgactacg agtacgacaa aaaagggctt gaggtaattt atttctgcga
    11221 aatactcaga aagctgagcg gcttgtacga ttacatcatt gtcaatgctg acgaaagttc
    11281 ggtaataaac actccataca tatataagac agccagcaag gtttttatag tacatgactt
    11341 catgcctaca aagataagaa ctacaaaaaa gctccttttg aaaatgatat ccgcaggcat
    11401 caacactcag aagacagtat cccttatata taacaagact cttaaggata ccatagatat
    11461 aggccggata gaagaaaagc tgcttttcat caggctgccc aacaggaaga tactgcctac
    11521 cgtgaacatc aactgcagta cctttgagat accctatgag cagagaacaa tggctgcatt
    11581 agtaaataat cttgcccaaa acaccaccgg catagaaaac acctccaaaa aatacaggca
    11641 tattattcaa aatatttata aatacataaa taacattccc tataccgaaa atgatgaaat
    11701 ggaaatctcc cagttcctca gggaaaagct ccggaagctt tccgacagga agtttatgct
    11761 ggaatacatg agaaatatct gcgatacctc actgataaag aattacatta aggcgtataa
    11821 aaaatagtac atctcccgat gtactatttt catattacgc cgtattccct tcctgccgtt
    11881 tccaaggcat ccagggcttt atccaggtgt tctttcgtat gggcggacat gaggctcatt
    11941 ctgattcttg ataacctttt gggaacagcc ggatacataa ccggattcgc atatatatcc
    12001 aattcatgga gccttctgca gatttccttt actttaaggt catttcctat gataatcggg
    12061 aaaattgccg tctccgaatt ccccaagtca aaccccaggg cggtcagatt agacctgaaa
    12121 taccttatgt tctcccaaag cctttccctg tgctccggtt cctcctctat cacatctatt
    12181 gctgccagca gcgagcctgc cacctgagga gtcattgccg tggaaaatat gtacggcctt
    12241 gaatagtagc gcagcagctc taccagttcc gaatttgcag ctatgaagcc tcccacccct
    12301 cccaaggctt ttgacagggt gccggcaaca atatcaacct ttccctcaat tttgtgatgc
    12361 tggggggtcc cccttccggt atctcctatt actcctgttg catgggcttc atccaccatt
    12421 acatatgccc catgggtttt tgccagctca gctatcctgt caagggaagc gatatccccg
    12481 tccattgaat atactccgtc aactgcaata agctttgttc tatacttatc cttcactctt
    12541 tgaagcaccc tttcaaggga gttcatgtcg ttatgcctga aaaaccttat attcgttgat
    12601 ttgcagccgt ctattatact tgcatgaaca taggtgtcaa gtattgcaat atccttttcc
    12661 tgaagtatgg aaagcaggct cccgcagttg gagccgaagc cggaggtata gatgattgaa
    12721 tcctcacagc ctttgaattt tgctatcttt ttctccagtt caatatgtat gtccaaggtt
    12781 cctcccaaca ggggaacact tccggcaccg gaaccgtatt tctccagcgc tttccttccg
    12841 gctgctatgg ttctgggatg cttggtaaga ttgagatagt cattggacgc caggtacacc
    12901 atttccttaa tctttcctgt ataatggtca acaaccttca ttaccggccc tgacccatcc
    12961 attgatattc ttctgtattg aagatgcctt ttctctctga tatccttcag atattctccg
    13021 aacatctttg ccctgcctgc catatcggca tcgacatcaa aaaaatccgc cagactgtga
    13081 ttccttgaat ccatatcctt acctcctatt ggtttttatt ctgcggttaa tcggatgtgt
    13141 acatttctgt gcacattatt atatatatta tgcctgtttt tctaatattt agaagtgcaa
    13201 ttcatctaat ccggcgggct tttcatatat tgacatatgc tgtcctgccg gtgataattg
    13261 gagtatatac aagaaaagga gcataaaccg ttgaaaaggc tattcagtat tgtgattgtc
    13321 gtgcttattt tattaatagc agcaagctgc accgttcctg atgtttcgga aggaaacact
    13381 cctcccgttc ccccggctca gagtgaccct gaggcagagg ataggaagga tcctcttgag
    13441 gcaaaggcag aatccttact ggccgggatg actattgagg aaaaagcagg acagctgttg
    13501 attgtggggt tccctggaga tacaaaaaaa gaagctttgc aggattatat agacaggtta
    13561 aaggtatccg gctttattct tttcagcagg aactatacgg attttgattc cctgtatgca
    13621 ctggccagga gcctgaagga aatgaattcc cttaataatc ctcttccgct gtttatttca
    13681 attgatgaag aggggggcac cgtatcacgg ctcccaaagg gcggtacacg tttccccgat
    13741 gcccggaagg tgggaaaagc gggagagccc gggctcacat ataaggcagg gcagactata
    13801 gcaaaagagc ttaaagccgc aggaataaat cttaattttg ccccggttct tgatattgtc
    13861 gaaaacggtg agaacaagct gcttataaag aggtcttacg gaagcactcc ggaagtggtt
    13921 tcgctgcacg gaacatcatt tatcagcgga ctgcaatccg aaggcgtaac agcagtgccg
    13981 aagcatttcc cgggacatgg aaacaccaat caggattctc atagtactct tccggttatt
    14041 gatacggaca aagctgcgat gcaaagcaga gagctggttc ctttcaaggc cgccattgat
    14101 gcgggactgg atgccgtaat ggtcgggcat attgcttttc ccaagcttga ccctacaggc
    14161 cttccggctt ccatgtcaag ttattttctt accgatgttc tgagaaaaga tttgggcttt
    14221 ggaggaattt ccatatcgga tgatattgag atgcaggggt acataagcag taaggatact
    14281 gttgaggaat gtgtaatctc ttcctttaac gcaggccttg atattttttt gatagggcac
    14341 acaaaagcca ttcaggatca ggtatataaa gctttactcg atggatgccg ggacggaaga
    14401 atatcagagg aaaggctcaa tgagtcggtg ctccggataa taaaggccaa gcttaagaat
    14461 gggcttacgg atgccatgga atatgaaatt gaagaggcaa aaaggatttt cggcagtgac
    14521 gagcataaag caattctgga ggaactgaat ggggacacgg ggacgtttct cctgtcccgc
    14581 tgaaagcggg acaggagaaa cgtccccgtg tcccgcgaaa cgtccccatg tccccatgtc
    14641 ctatgtctca tccttcagat attccatcag ttcctttacc agattgtact cggtaatcaa
    14701 atccgaatac cggagctgcc tggctgcgaa ctccttctcc tttgcaagct tttccgcagg
    14761 gtatgtgctg ccggtttccg catcataagc aacacccttg gaggtcaggc aaatgtactt
    14821 gtcggtaata aaatacccat ggcgcagcac tgccattcct tcttcagaat tcagcaaatc
    14881 cctgcccagc atcggtatat tgctgctgtc aacccccatg atgttcaata ttgtcggcat
    14941 gaaatcaagt ccgccccctg ctatatgctt tgtccctgta tattttccct ccggaatgcg
    15001 gataatcaga ggtatcctct gctgcttcac ccaattgtaa tcatccatct ccttcaatcc
    15061 gaggaagtcc gccaggtatt cctttttgtc cctgggtatt ccgaaatggt ctccatatac
    15121 ggccaatacc gaacggtcca atatacctct tgcctccaac tcatctatga accctccaag
    15181 ggcttcgtca gcataatgaa tgacctcaag atagctgccg aagaaagtac ccttgtactt
    15241 gccggcatta aagctgccca agtatttttt gtcattgtcg taaggaaaat gactggtcag
    15301 agttattaaa aaagtatagt agggttcctt gtatttctcc ataaattcaa gagactgcct
    15361 gaagaaggat ttgtcggtaa gccccattcc gctgatctca tcgggaatat aatcctcctt
    15421 gtcaataaac tcatcaaagc caagggtctt gtacatcacg ctcctgttcc agtagccggg
    15481 tctgtaggcg tgcattgcag aggtcaaata gccctcttcc gaaagcctcc tggcaagtga
    15541 gtaataatcg ttcagcggga atctgatgta tgtagctccg tctctcgcag gatgcatcga
    15601 tacgttggac agaagctccg catcggatgt tccccctcct gccgtctgtg tataatagtt
    15661 atcaaaatat aagctgcttt ttatcaattt gttcagattc ggggtaatct cctgaccctc
    15721 tatgctcttt cctatgacaa attcctgcag tgcttccacc tgaaccacta taagattctt
    15781 gccttttccg gttccgaaaa gcttggatga tgacggttgt tcaggctttt tatccttaat
    15841 gaattcagct atttccttct ttatatcctc cgatagggac agttccaatt tatcctttga
    15901 ttttacatac cggaaggcat ctatgccgtg aaagcccagc agccctatat tctgaacaat
    15961 atataccctg tcataaaatg acctgaatat ttcgggctgc gccttcagta tttccgacac
    16021 cccgcatccg cacagcccga tacctatggc aagggctgac aaggcgcaga tgattctctt
    16081 tccgaagcct gccttgggtt ttttattcaa cctgcctgcc ttggccgata caaaaggcaa
    16141 aaacagaaga tcggcggcaa atataaggtc ggatgcatgc aaaaggttca gaatgctgct
    16201 tttcaccgaa gtgacaaccg aagcctgctc caatacgggc attgaaagca cgtcgctgaa
    16261 atacctgaaa aacaatagat ccgccattat tatgaatgtt acggcacagt caagcataaa
    16321 aagaagcggc agccggattt tccccggcag caggccggcc cagaaaaaca gcagcaatac
    16381 cgttccggca acggaagcaa ttaaaggaat gcttcccatg cctgtcccat ctattatgct
    16441 gttaaaaaac aggagctttg ccgttatgaa aataaaataa aggaaaacat ccgcattcat
    16501 tgtatcatcc tctgattaac tgcattttgt tatgtattca tactatttca acaaggatat
    16561 tctacagcaa tcaagagaat tatacaagct gtgcttaagc aatgagggct gcacttatac
    16621 aagaagaggg atatcgccaa ctacctatca agtagttttg cgatatcctt ttacagcatt
    16681 tccttaacac cgcttgccgc tgccctgaag tgcctgttgg catcagggaa atgaatcggg
    16741 tgtttttcta tagttcgtgt atgaataatc cgcttcttag ctttggctca aaccaggttg
    16801 atttgggcgg cataaccttt cctgcatcgg ctatggacat taagtccata atagtggtag
    16861 ggcacatgga gaaagcaact ttcataccgg catttaccct tctttccaat tccccgaggc
    16921 ctctgatacc tcctacaaag tctattctct tgtcggttct gggatccttt attcctaata
    16981 cagggtcaag caggttattc tgcataattg acacatccag cctgtcaacc ggatcctctt
    17041 cattgtaagt gcctgccttg gccgtaagct tataccactt tccatccatg tacatcccga
    17101 aggtgtgcct gacagtgggt ctgaattgtc cttccccagt gtattcttca atgtcaaatt
    17161 tctctccgac ttttttcagg aactcttcag cggaattgcc gttcaagtcc tttaccactc
    17221 tgttgtaatc cattatataa agctgctcat cggggaaaag taccgagagg aagaagttga
    17281 attcctcgtc ccccttgtag tccgggaagc tctctctccg cttgaggcct acctttaccg
    17341 cggatgcgga acggtggtgc ccgtcggcta tgtataaata gtcaacggct cggaaccttt
    17401 cttccagcct ctgaattgca tcatccccgt caattaccca taccgtatgg cctataccgt
    17461 catcggatac aaagtcatat accggcttgt ggtcagccat ccagttgtcc actatttcat
    17521 ttatttcctt tgaagccctg tatgtaagga aaatcggccc ggtattggcg ttgcagtaat
    17581 caacatggtt tatcctgtcc tgctccttgt ccgcccttgt atactcgtgc ttcttgataa
    17641 tgttgttcat ataatcatct attgaggtgc agcccacaag ccctgtctgt acacgcccct
    17701 tcataacctg cttgtatatg tacaggcagt tcttttcatc ctgctgaaaa actccgttct
    17761 taatcatttc tctcagattg tccctggctt tttcatatac ccttttgtca taaagatcaa
    17821 tggaagggtc aaggtctatt tctgccttgt caacatgcag gaatgaatag ggattgcctt
    17881 ttaccattcc tcttgcttca ttgctgtcca ttacatcgta aggaagggct gccaccttgt
    17941 gcgccagctc cggtgccggc cttatagccc tgaatgcttt tactgttgcc atagttttct
    18001 ctcctattca aagaaatttt gaataatctg tattatttcg ccgcctattc tttcctgagc
    18061 ctcattggtt gaagcaccta tatggggcgt caatgatatt ctctcatgag tgtatattct
    18121 ttcattcttg gtgggttcct cttcaaatac atcaagagcg gcggcggcaa cctttccgga
    18181 gtcaagagcc ttcaagagcg cttcctcgca tacgacaccg cctcttgccg tattgaccag
    18241 atatactccg tccttcattt ttgcaaactg cgcttcacct attacaggct gctggtcctg
    18301 tgagcagggt atatgcatcg atataaaatc ggagacagcc agaagttcgt ccagtttcat
    18361 atatttgtaa tcgttgaagc cttcctttgc tccgctggta ttggtataaa caaccttcat
    18421 tcccaaagcg aatgcccttt ttgcaacctc ccttgcaatt cttccaaagc ctacaaggcc
    18481 cagggttttg ccggaaagct caatcccctc gtactgcttc ttgttccatt tgccttgcct
    18541 catggtatga ttggcaatat atatatgcct tgccaggcag aacatatggc ctatagccag
    18601 ttccgccacc gatgcgctgc ttgcattggg cgtattggca actgctatac cgttaagctt
    18661 tgcataatcc acatctatat tatcaactcc aacgccgccg cggattatga gcttaagcct
    18721 tcctgtttcc agagctgcat ctattatcgg ctgccttact tttgttgccg aacgcacaac
    18781 aactgcatca aagtttttta tttgctcctt taacacttcc ggctcgtaaa attgttcaac
    18841 cacctcatag ccgctgttct taagcttttc aacagcggat ttttccatac cgtcagttac
    18901 aagtatcctt ttcatctgtt ttcaaccccc aagctttatt tgttttctga aagtccctct
    18961 tacagtccca aaatctcatt gatattctta ataagctcct tcacttcacc aagagtgtaa
    19021 tcacccatat gggatattct gaaggtcttg tccttaagat ctccataacc gtttgaaatc
    19081 ataaagcctc tctcacccag ctttttgttc aggccgccga catcaattcc tattgtattc
    19141 gtaacggtag taagggtatt tgaaaggtat ttctcatctt ccacaaacag cgcaaagttc
    19201 tttcttgccc atgcacgtac atattcagcc atctcgatat gtctggcaaa tctcttctca
    19261 aggccttcgg caagaatctt atccaactgg tagtccattg caaacatgtg ggcaagggac
    19321 ggagtcgaag gatattgatg atcctttttc tgtatgaaat cataaagctc taccaggtca
    19381 aagtacatcc ccctgttctc aacctgtctg gcggcttcaa cagccctttc ggatatcgag
    19441 catgcagaca tgcctgccgg caagccaagg catttctgtg ttgatgtaat gcagacgtcc
    19501 gctccccact cgtcaactct gatgtctgtt cctcccatgg agcttacggt atctataaga
    19561 taaagcacat ccggatattt cttcttaaga agctctccga tttctccgca gggattcata
    19621 atgcctgccg aggtttcatt ctgggttact gtgattacat cgtatttgcc ggtggcaagt
    19681 gcttcatcca ccatttcagg agtagtaggc tttcccggta ccgatgagat tttatccgcc
    19741 ggtaccccgt tggctttggc catcttgaac catctgtctc caaaggcacc tactgaaaat
    19801 accgctgctc ttttcctggt gcaggagcgg atggcacctt ccatcaggcc gcttccggag
    19861 gatgtggaga gtattatctg gttcttggtc tgaaatactt tcataagctt ttcagaaaca
    19921 cttcgctgta atgccgatgc ctcctttgtc ctatgcccta tctgaggtgt agccattttt
    19981 tgaagcacat cctcacgtac atcgataggc cctggaataa ataatttttt gtgcattttc
    20041 gttcctccct taactctttt ctaaaattat aatttaataa tttataaccc aagataataa
    20101 taccatttat gggaataagc ctcaacacat ttaaattaat taaagacatt ttttgcccgg
    20161 tgtcctcaat ccgggcacag atggccaatt tgaagtattc aaatattcag cggcatttgt
    20221 tataattgat atatgagaat atcccttcac catatcattg caaagaggtg tcatatggaa
    20281 atggatttta caaaactggt caacaggaaa accgccatat gcgataaaga agtatatctg
    20341 gaattctccg tactggttcc gataataaaa accggcagtg agttttcact tctttttgaa
    20401 gtcagatcgg agaagcttgc caagcagcct aacgaaatat gcttccccgg agggaagata
    20461 gaaagctttg aaaatgcacg gcaggctgcc ttgagggaaa ccacggagga gcttctgatt
    20521 cctgaagcga acattgatgt cattgccgaa ctggacacta ttgtcacacc atttaacaga
    20581 atcctgtacc ccttcgccgg aaaaatccat gaatacaacg gaacctttaa ttctgaggaa
    20641 gtaaaggaga ccttcattgt tcccctttcc ttcttcctgg aaaatgcccc gttgcatcat
    20701 gatattaata taagtatgaa acctgtggaa ggttttccat atcatatggt gcgggacgga
    20761 agggattacc cctgggggaa agggaaatac cctgtctatt tctataccta tgaaaataag
    20821 ataatatggg gactgactgc aaggattatc aaaaactttg taaatctgct gggttcataa
    20881 tatgtacccg gaatacaaaa gaaggagtat caacagcatg gaaattaaaa gaatcaaagg
    20941 tgttatacct ccaatgatca cccccttcaa agaaaacggg gatgtggatt atcggatgca
    21001 tgccggtaat atggagaaat ggaacaatga taacctggca ggctgcctgg tcctgggatc
    21061 caatagtgaa accgtatacc tgaatgagga agaaaaactt gagctgataa ggattactgt
    21121 tgaagcggca agaaaagaca ggcttatact tgcgggaacc ggcatggaat caacaaggga
    21181 aacaataaag ctgactaata aggctgcgag gcttggggct cacggtgcat tggttctgac
    21241 tcctttttat tacggcggta aaatgaatga tgaagcnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnggtt caggaatact atgaaaatgg ccaaattgaa aaagcaaaag
    21421 acacatatac caggatattt ccagtaaaca cagcggttac agccacctat ggaattgccg
    21481 gcctgaaata cgcatgctcc ctgtcaggat atgaaggggg ttatgtaagg aagcccctgc
    21541 tggaattaaa agaagatgaa aagaaaaaag taagggatat tctgtccaaa gccggactta
    21601 tttagggaca tggggacagg taccgtgtct ctgtcccttt atccatatcc tctgctcatg
    21661 ctaaaacatt ttcaaactcc tgcaaatccg ttataggagc cctgcaggcg aagttttcgc
    21721 atacataggc agttgttttg ttatctacca tcccttgagc ttcagtataa ggggcaatgc
    21781 ttgtaatacc ttcttccgca ttttctttat ccttgaacag gacaacggta aatgggctga
    21841 aatggctatt tatacttttt atcattcttt ttgcattctc atcctcacga ttgcctacta
    21901 tcactatttc acttgctttg ctgttaataa aaagcaaagc cgacagcata tcggcatatc
    21961 ccatcgggtg gccttttata atacttccga aagcttcaag ctgcttataa gccttgtctt
    22021 ccaaatcagt atctcccgta agccttccaa gcctcaaaaa attcatggcg gcaaccgaat
    22081 tgcccgaagg cattgcaccg tcatatacat ccttaggcct gacaatcagc tgctcgctgt
    22141 cgcttccgta aagaaaaagc cctccctttt catcgtcata gaaatacttc agcatatcgc
    22201 ggttcaagtc tgcagccagc ttcaaatact cggccctgaa ggttgcttca tacagctcta
    22261 taagccccca taccagaaat gcatagtcat ccaggtatcc cggataagcg gcttctcctt
    22321 ctctatatct tgcaagcagc cttccgtcct cccgtataag ctttgatccg ataaacctta
    22381 cagccctttc agctgcaacc gcatattcca tgttatccag cacccttgct ccaatggata
    22441 gggctgctat catcaagccg ttccatgctg tcagtacctt gtcgtcctta tagggatgaa
    22501 tcctcttttc tcttgcttca aatacctttt ccctatgacc ttccaagggg tttttccgga
    22561 tattttcaaa agcagcttta ttatgcagaa ggttggggat gtttttcccc tcaaaatttc
    22621 catcgtcgct taacccgtaa tagccgttga actccactgc agcgtcctcg cccaaaatgt
    22681 tcgttatctc ctttgggctc catacgtagt acttcccttc caccccttcc gaatcggcat
    22741 cctccgcaga gtaaaaggct ccctccggtg aagtcatatc cctgagcacg tactcgaata
    22801 tctgccttgc aacctccccg tactgctcct ttccggtggc ctggtatgct tccaggtatg
    22861 cggcagccaa acctgcatta tcgtaaagca tcttttcaaa atgcggcacc agccaaatgc
    22921 ggtcggtgga gtatctggaa aacccataac ccacatggtc aaagatgcct ccccggtaca
    22981 ttgaaatcag agtcttttca accatctgca aggctttggg gtcatttgtc accttccagt
    23041 acctcagcaa aaaccgcaga ttatgaggcg tcgggaactt tggtgctctc ccgaaccccc
    23101 catattcact gtcgaagctc ctttccagac ctttgaatgc tttgcctgca gtttcaatgt
    23161 caagctcaac cgcagaatca gcagcagaat cggatctgat atgctccaat atattctccg
    23221 aggcctccaa taatttttcc ctctccgtat tccaggcctc attgattttc tccagtaatt
    23281 caatcactcc cggatagccc atccttgccc tcttggggaa atatgtgcct gcaaaaaatg
    23341 gggccttttc cggagtcatt attatggtca ggggccatcc gccgcttccc gtcaaagcct
    23401 ggcacacatt catgtaaata tggtcgatat caggcctctc ctccctgtct accttaatgg
    23461 ctatgtagtt tttgtttaat atctccgcaa cttccttatc ctcaaaggat tcccgctcca
    23521 tcacatggca ccaatggcag gtactataac ctaaactaag aaaaacaggc ttatcttctg
    23581 cctttgcctt atcaaatgct tcctgtcccc aaggatacca gtcaactggg ttataagcat
    23641 gctgcagcag gtaaggggat ttttcagtaa tcagtctatt ggctgctttg tctttattca
    23701 tgcgggcatc acc
//
LOCUS       DAOOAB010000039        23706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2791_length_23706_cov_16.269249, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000039 DAOOAB010000000
VERSION     DAOOAB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23706
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_23706_cov_16.269249"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..2211)
                     /gene="fusA"
                     /locus_tag="PKU88_08960"
     CDS             complement(142..2211)
                     /gene="fusA"
                     /locus_tag="PKU88_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPX69442.1"
                     /translation="MPRQFSLEKTRNIGIMAHIDAGKTTTTERVLYYTGKTYKLGEVH
                     EGTATMDWMEQEQERGITITSAATTAEWKDHKINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFCAKGGVEPQSETVWRQADKYNVPRIAYINKMDIMGADFYRVVEMMKERLGANAV
                     PIQLPIGSEDRFVGIVDLIGMKAHIYKDDLGKNMEVGEIPSDMMDKAKEYREKLIEAV
                     SEMNEDLMMKYLEGEEPTEQEIKDAIRAGTISVKINPVVCGSSYRNKGVQLMLDAVVD
                     FMPSPIDIPPIEGTTVDGEEKIVRHSDDKEPFSALAFKIMVDSYVGKLAFFRVYSGMV
                     EAGSYVYNSTKGKRERIGRIVQLHANHREEIKEVYTGDIAAIVGLKDTTTGDTLCDEE
                     NAVILESMDFPEPVINVAIEPKTKAGQDKMGIALQKLAEEDPTFRTYTDAETGQTIIA
                     GMGELHLDIIVDRMMREYKVEANVGKPQVAYKETIRKKVQVEGKFVRQSGGKGQYGHV
                     WLEIEPKEPGTGFEFVNKIVGGVIPKEYIPAVQRGVEEATMNGVLGGYPVLDVKVTLY
                     DGSYHEVDSSEMAFKIAGSMAFKDGMKKADPVLLEPIMKVEVTVPEEYMGDVLGDINS
                     RRGKIEGMEARSGAQVIRGFVPLSEMFGYATDLRSRTQGRAVYSMETDHFEEVPKSIA
                     EKILGEK"
     gene            complement(2320..2790)
                     /gene="rpsG"
                     /locus_tag="PKU88_08965"
     CDS             complement(2320..2790)
                     /gene="rpsG"
                     /locus_tag="PKU88_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPX69443.1"
                     /translation="MPRRGHISKRDVLPDPLYNDKIVTKLINNVMYDGKKSVGQRIVY
                     GAFEILREKTGKDPLEVFETAMNNIMPVLEVKARRIGGATYQVPIEVRPDRRQALGMR
                     WLIEYARKRGEKTMKERLAGELLDAANNTGSSVKKREDTHKMAEANKAFAHYRW"
     gene            complement(2922..3296)
                     /gene="rpsL"
                     /locus_tag="PKU88_08970"
     CDS             complement(2922..3296)
                     /gene="rpsL"
                     /locus_tag="PKU88_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPX69444.1"
                     /translation="MPTISQLVRKGREKVEYKSTAPALKESPQKRGVCTVVKTTTPKK
                     PNSALRKIARVRLSNNIEVSGYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DAAGVADRKQSRSKYGAKRPKK"
     gene            complement(3417..3659)
                     /locus_tag="PKU88_08975"
     CDS             complement(3417..3659)
                     /locus_tag="PKU88_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPX69445.1"
                     /translation="MQELKAIHRRTVGLKQTVKAIKSGTVKKVYLAEDADDFIKQSVL
                     DVCRDRDIQIIYVSNMKELGDACGIDIGASTAAIKD"
     gene            complement(3859..7383)
                     /gene="rpoC"
                     /locus_tag="PKU88_08980"
     CDS             complement(3859..7383)
                     /gene="rpoC"
                     /locus_tag="PKU88_08980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPX69446.1"
                     /translation="MFELNNFEAIKIGLASPEKIREWSRGEVKKPETINYRTLKPEKE
                     GLFCEKIFGPTKDWECHCGKYKRIRYKGIVCDRCGVEITKAKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRALEKVLYFASYIIIDSGDTPLSKKQLLSEKEYRENIE
                     KFGNRFRAGMGAESIRELLLDLDLEKLSKELRQDLKGSTGQKRLRTVRRLEVVEAFKN
                     SGNKPEWMILEAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKLFQCGLPNEMALELFKPFVMKKLVNDGLAHNIKSAKRMVER
                     VKPEVWDILEEVISEHPVLLNRAPTLHRLGIQAFEPVLVQGRALKIHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSANNILAPKDGRPVVSPTQDMVMGVYYLTIYVPG
                     HKGEGKEFTDLNEMLMAYQLDEVSLHAKVKVRITKEINGRMVSRFVENTVGRFIFNQS
                     IPQNLGFVERNTEEDMFLLELDPRDKDGNLMPVDKSLLGKIVDKCYRMHGNTNTAIVL
                     DEIKRLGFHYSTKGAITISISDMDIPMAKKELLSKADERVDKIEKMYRRGLISNDERY
                     EQVIETWKIATEDVTKALMDNLERLNPVYMMANSGARGSVNQIKQLCGMRGLMADTSG
                     RIIEIPIKANFREGLDVLEFFTSTHGARKGLADTALRTADSGYLTRRLVDVSQDVIAR
                     EEDCGTEDGLMVSEIKDGNEVIEKLKDRLMGRYASSDILHPETGEVLVKRNELITEKK
                     ANSIVKLGIKKVPIRSVLTCHSKYGVCTKCYGVNLATGDPVHIGEAVGIIAAQSIGEP
                     GTQLTMRTFHTGGVAGDDITQGLPRVEELFEARKPKGLAIISEISGVVKIIDQRKKKD
                     VVVLAEDGDSRTYTIPYGSRLRVADGDVIEAGDEITEGSVNPHDILKIKGVAGVQSYL
                     LQEVQRVYRLQGVDIADKHIEVIVRQMLRKVKIEESGDTDLLPGGLFDIFKYEEENQR
                     VSAEGGEMATGKRVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKIDPLVGLKE
                     NVIIGKLIPAGTGMKKYMDISIKQVEAE"
     gene            complement(7410..11459)
                     /gene="rpoB"
                     /locus_tag="PKU88_08985"
     CDS             complement(7410..11459)
                     /gene="rpoB"
                     /locus_tag="PKU88_08985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPX69447.1"
                     /translation="MLVHPISAGRNQRISYSKIDEVMDMPNLIEVQKNSYNWFLEEGL
                     REVFNDVSPIQDYTGNLILEFVDYSLDGKPKYSVEECKERDVTYSAPLKVKIRLINKE
                     TNEVKEQEVFMGDFPLMTDTGTFIINGAERVIVSQLVRSPGVYYAETIDKSGKKLYSS
                     TVIPNRGAWLEYETDSNDILWVRIDRTRKIPITVFLRALGFGTDEQIDGILGEDDRIK
                     ATLQKDGTKNEEEGLIEVYKRLRPGEPPTVESAKSLLEALFFDAKRYDLAKFGRYKFN
                     KKLSLSSRLTGQKLDRELADPRTGEILAEEGEKITRDKAVKIQNAGINEVYVTAEGKT
                     VKILGNNTVDINKYLSFDVSDLNIKELVNLSVLKDILQKAGSESEIKEEIRNRIKELI
                     PKHILIDDIVASINYNINLSNGIGNKDDIDHLGNRRLRSVGELLQNQFRIGLSRMERV
                     VKERMTIQDIDVVTPQQLVNIRPVAAAIKEFFGSSQLSQFMDQTNPLAELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLIGSLSTYAKVDEYGFIETP
                     YRTVKTDDNGEKYVTDEIVYLTADVEDEYVIAQANEPLDEEGRFVNRRVTARDANEIV
                     IVPTMEIEYMDVSPKQVVSVATAMIPFLENDDANRALMGSNMQRQAVPIIKPDSPIVG
                     TGMEYKSARDSGVIGLANSDCVVEKVSANEIVMRNSLGEKERYKLLKFLRSNQGTCIN
                     QRPVARKGEVLKKGDIIADGPSTDMGEIALGRNVLIGFMTWEGYNYEDAILISEKLVK
                     EDTFTSIHIEEYEAEARDTKLGPEEITRDIPNVGEEALKDLDERGIIRIGAEVRAGDI
                     MVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGESGIIVDVKVFTRENGD
                     ELPPGVNELVRCYIAQKRKISVGDKMAGRHGNKGVISRVLPEEDMPFMPNGTPLEIVL
                     NPLGVPSRMNIGQVLEVHLALAAKALGWKIATPVFDGATEESILETIECANKVLQVKK
                     NLRIIKERILIADKQNDEDYEGQQLAETDIRSEIKENVEIIYELLNHEIYDIDSKFTS
                     LREMIKSSDLTELKSVEKILKAFGETPIDGIELNHTGKATLRDGRTGEYFDNDITVGY
                     MYILKLLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGSAHTL
                     QEILTVKSDDVVGRVKTYESVVKGENIPEPGVPESFKVLIKELQSLALDVKVLTEEHE
                     EITIRESVEEDVEDLGADMEVREDMDFSSLSYKGETVTPEDLYEDYEMESEGAEDFDA
                     EIDLDDLLIGDNEVEEEVEEEVEEDGEEF"
     gene            complement(11753..12646)
                     /locus_tag="PKU88_08990"
     CDS             complement(11753..12646)
                     /locus_tag="PKU88_08990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPX69448.1"
                     /translation="MIIGDVLNGKYRIERLLGKGGTGSVYLCTNIEIGNKWAVKHIPP
                     DKINDKTLSEIEILKRLYHPSLPRVVDAFRNEQGIYIVESNIEGSSLDKLLKRHGSFD
                     LETVTDWFLELCDILKYLHGMKPRPIIYRDMKPSNIILAQGVRLVLVDFGISQEYCEY
                     ESGDTFFAGTSAYAAPEQLINGGKTEQRTDIYNMGVTIYHLLYGRLPGSADYSLRRRK
                     SRAASNVGRIISKCMENKPEDRYQRVEDIQKELESVKNMLAVNKARQKAVFKLEIASI
                     ALLSAASYTAAILGALYSLSK"
     gene            complement(12673..12888)
                     /locus_tag="PKU88_08995"
     CDS             complement(12673..12888)
                     /locus_tag="PKU88_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69449.1"
                     /translation="MKLHMIYISIGVFTLILNLALTLHIIRGHRNKSCRKNPDCEEVI
                     TFRLTGAEGIEDATTAAAPCDDTLPLE"
     gene            complement(12915..14252)
                     /locus_tag="PKU88_09000"
     CDS             complement(12915..14252)
                     /locus_tag="PKU88_09000"
                     /inference="COORDINATES: protein motif:HMM:NF039422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HPX69450.1"
                     /translation="MRNTVLDFEYSYDNNAAGSYLVLKIDSESALLKHQVEILCQNPN
                     SAFMPFHVRRENENTFIYYNITSKISLSHYIERKSLNRAGLLDLLRSIAKNLMLSGNY
                     LLDLSGFIIHPDYIFINPATAEASLLYVPVSCNRNVTEILRSFFKDIVVNSSNADDNA
                     RDNYLQRILNYLKSDTFSLIDFNRLIVDLRSSGELYEYGQGAAVQEAAAGISTPSSRS
                     PKERAYEDAGKSSNIRSIMLLQLIFLVAAAAACLFLISRKMGDIVSIAGVVIISAALD
                     ILVINRISGSRGKRAATDDTQRIAQDKVKRSAGRHDEVLRSRYKPVFSSPDVVKACDT
                     VCISEIPKDCRPYLESIGPNRVERVIINKDGFIIGRLGSMADHIIQGGTIGKLHAEIT
                     FNEGFYYIRDLNSKNGTFVNGVKIPSNREYGIKDNDRIKFSNYEYVFKMQEEL"
     gene            complement(14274..14702)
                     /locus_tag="PKU88_09005"
     CDS             complement(14274..14702)
                     /locus_tag="PKU88_09005"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPX69451.1"
                     /translation="MSHYLPYLTIIILLAAAAIIDIRKKRIPDKLVLAGTIAGMAFVL
                     IDPQREFLNSILGGVTAGLVLLLIHYITKGGLGLGDVKLFGCSGIFLGFERIISAMLI
                     SAILSGLFSIVLICLNPDNKKSEIPFAPFILAGVLAVIIP"
     gene            complement(14728..15669)
                     /locus_tag="PKU88_09010"
     CDS             complement(14728..15669)
                     /locus_tag="PKU88_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69452.1"
                     /translation="MYKGYKKLRSRGSITVEACAVFPVFISVFFLLLFLIKFTCTGMV
                     LDHVVNEAAKEIAAAAYPISFINELEDEKHREYGNAKIPTLEEELEKLAGEISGGSSD
                     NLFRAIVSGELEGTDLTEAVQDILKDYGKGAIGNVVGRITPAYWDMKSDAKYYIAEAF
                     IKEHLKSPLINRENIRLRLVEFPQGEEEYNARSQRAIYSELGLTPDKDFSRDDVVLQL
                     EYEYLVRLPFIEPFNIRMIHTAVERAWINGSFGILTAEDEGLDLEPEGSIVYITRTGI
                     RFHEGDCRYLRKSRIPILIEEAEGSGYTPCKVCKPLG"
     gene            complement(15681..17735)
                     /locus_tag="PKU88_09015"
     CDS             complement(15681..17735)
                     /locus_tag="PKU88_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69453.1"
                     /translation="MYQRDGSIRKEKGAITVFLALIFMSLIIFAGTVIDIVRIIAADR
                     RVQSTLNTSARSVLADYDSELMGSYGIYGVNAAEGNVKEDFYRYLNVNLKERHKNINF
                     INISIDYEDIEIQGMENLLKHETFNRQVQEYMKYRTVISATGSLIEQLQNIKLNKGIE
                     FAKSEKITRDKANELRAKVSEVNIKLTSIKKKLTNISAEKLEDIRDELSGALEINSLI
                     CNGNAGGLLADYIQSRDETNIKAEEGQFVENQSREFDSIKKNNENLTPGIQMYIEEID
                     KTLLLVKPLQNELERMEGKLESLKDELDELKEKLSDVQEDDAGVAEEIDETREEIDEV
                     KDKIRRLESEIDEEMSDLKMKLGRISLEGYSLKEEAVQLPDKKTEELKSFISKTKKEI
                     KEALFRRLEKGWLIEPEEFEDSSLITGENFGEMDESTYFSASMREEEAEKSNDAIIKT
                     LEKLAKAIGDVASNAFEKVNTIEYVMDKYTFLTSGTERGHYFRKSEVEYIISGSDIEE
                     GYSPIKNTEYYVVTKVLLQVWALRFALDTIDAFIRSTVVFPPQRLAFALAEGALDSSL
                     DIFNMLNGEAVPICPKSFTTVRLKYSDHLKILLLMKPEEEILRRARQLIQVNIKQVVD
                     AKTELPRSDFRLGDYSTVISASVKAKVNLFFLPLLKVDRLLPGSFENGKYLIYKQIYV
                     GY"
     gene            complement(17765..18466)
                     /locus_tag="PKU88_09020"
     CDS             complement(17765..18466)
                     /locus_tag="PKU88_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69454.1"
                     /translation="MLDKHICDRGSYTVEASMVFVTIMLALLALLFTFLYMGQRASLE
                     SFASFAAQQGAELWLDSRRNMEDGKINNKRPEDSMGYRIFDNLLFSSTTYEGYFEREA
                     SDNGKTRVVFKMDAGNNLPGHKALLIGEDLCKRIESTILKPESTRIRITFNNNALRGR
                     LIVELIQEIKVPLGGIKEFFDGKDTLTLSVQSEAAVSEPDEYIRNVDLILELSKRLGE
                     ELDLGELAGRIGAKR"
     gene            complement(18515..18727)
                     /locus_tag="PKU88_09025"
     CDS             complement(18515..18727)
                     /locus_tag="PKU88_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HPX69455.1"
                     /translation="MLKEVKGFIVEEDGISTVEIVVIIAILIGIALLFRKSITAYVQR
                     VIRNFIDTAPAVENNEIDMTAPLPTD"
     gene            complement(18772..19635)
                     /locus_tag="PKU88_09030"
     CDS             complement(18772..19635)
                     /locus_tag="PKU88_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPX69456.1"
                     /translation="MLPVFITEIIVLGFICFYVKGKYEDFTETGFKKGHIYKVLAPAA
                     LFITDKLIAGRFIAYERKLGIKLAELYGGRDIKHMLKLHFVHKVIHMIIALLSVTLLG
                     ALMEKPDIIYAIFALSALGIVFYVSDRELDEKIKKRNFNIRYDFPDFLNKMVLLINAG
                     MTVPRAWEKIVRDRKNITPLYEELRTTYIEIMNGKPEMEAYEDFAKRCRVKEITKFIT
                     AVIQNLKKGNSDLVPLLKLQSNECWQLRKNMAKKLGEEASTKLVLPLMIMFVGILIIV
                     TLPAVMQLRYL"
     gene            complement(19639..20454)
                     /locus_tag="PKU88_09035"
     CDS             complement(19639..20454)
                     /locus_tag="PKU88_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPX69457.1"
                     /translation="MDLRKWPAVSTEYEYGRGTAAHASLADYDTYIMSLKEKLLCIFM
                     AGTVIFATGYIFYRSALLSLLLTPFALLYPGFRTKEIIKKRKNELNLQFREALYSLSS
                     SLSAGKSIETAIKGSIKELSIQYPNNDTYILIELEHISRRIEMNETIEEALADFAARS
                     HLEDIINFADVFLICKRTGGNLVQVVKNTAEIITEKIDIRQEINLLLMEKRLEHKVLN
                     LMPVLIVLLLSASAEEFMAPVFSEPLGRAAMTFSLVLFGAAYFVSRKIMDIEV"
     gene            complement(20466..21704)
                     /locus_tag="PKU88_09040"
     CDS             complement(20466..21704)
                     /locus_tag="PKU88_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HPX69458.1"
                     /translation="MDDDAKNLVINNLRKEIRESLDIKSQVTDEDINELITDKVFEEA
                     RLRSLSIREKQELISVLFNSIRKLDILQPLIDDKSITEIMVNGEDNIFIEKHGRTYSI
                     ESSFESRQKLEDVIQSVVSKVNRVVNEASPIADARLADGSRVNVVLPPIALNGPILTI
                     RKFSERPISMKDLIEWGSITEEAAEVLERLVKAKYNIFICGGTGSGKTSFLNALSDFI
                     PRDERIVTIEDPAELQIKNIKNIVKLETRNANIEGKGEITIRDLIRASLRMRPERIII
                     GEVRGAEAFDMLQAMNTGHDGSISTGHGNSTADMLSRLETMVLSGAMMPLEAIRQQIA
                     SAVDIMIHLSRFRDSTRKVVEIVEVTDCREGMIGLNPLFEFVERGGSMSEKVEGCLVR
                     TGNTMKNLAKLRMAGLNEVI"
     gene            complement(21783..22958)
                     /locus_tag="PKU88_09045"
     CDS             complement(21783..22958)
                     /locus_tag="PKU88_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69459.1"
                     /translation="MARLNLVIADCDESYARGLFEYINSNHSSSFMVSCFTKLDSFAG
                     YFEKQPQTDILAISSDFYDISVEYPNIKLKVILSGEALNREYPGFQAIRKYSTGDKLI
                     RDLLYLYSRANPQEIRLSSCSKDTEFIGVYSPAGGTGKTTIATSLSTQCAELGLKSFY
                     LNLESIQSTGMFFGLGSKRNLSYVYYYIKEKSRNLSFKMEGIKSTDDGVEYFNPPDSP
                     MEYEEINPEELEQLIKGIKGMGCCDYVFIDMSSNFDSKNYKIMKLCDRIVLVTLREPI
                     ASYKERVLLNELAKLRDTDGGSISDKFITVINKYKNKGDEDFESFGENAAAAVKIPEY
                     SRTLIREDGRIFIENDDFRKAVNRIIDIITRRKDVTTSTSDGNPFRCPTGSEHISPR"
     gene            complement(22961..23500)
                     /locus_tag="PKU88_09050"
     CDS             complement(22961..23500)
                     /locus_tag="PKU88_09050"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HPX69460.1"
                     /translation="MQLGVIAIVFKLIIVSMLMLLALVSDFRTYSVKNYITYGFMLIG
                     LAVNLAIEGIGGLVFSLQGIIIPAACLFVLYITRIVGAGDIKLLCAVGAVMGAAFALY
                     AAIYSFIFGGFIASGLMLTRENASERFNHLFVYLKSCFLTMDILQYTDLKNRQDGGKF
                     HFSIAIASGTAAAIIMRGV"
BASE COUNT         5941 a         6032 c         4038 g         7695 t
ORIGIN      
        1 tattgtccct atgtttacat ggggtttggt cctttcatat tttgcctttg ccattttact
       61 tttcctcctt taaaatcgga taattcaata tcttaggaaa ttgtacaata tcaaattgcc
      121 atacgatttc cctgttttta atcacttttc acctaatatc ttctccgcta tgctcttagg
      181 aacctcttca aagtgatctg tctccattga gtatactgca cgtccttgtg tccttgaacg
      241 caggtcggta gcataaccga acatctcgga aagaggtaca aagcctctta taacctgagc
      301 cccgcttctc gcttccatgc cttctatttt ccctcttctg gagtttatgt ctccgagaac
      361 atctcccata tattcctcag gcactgttac ttcaactttc attataggct ccagaagaac
      421 aggatcggcc tttttcattc catccttaaa agccattgaa ccggcaattt taaatgccat
      481 ttcggaagag tcaacctcat ggtatgaacc gtcatacagc gtgactttaa catcaagtac
      541 aggatatcct ccgagtacgc cgttcatggt ggcctcttcc acaccgcgct gtacagcggg
      601 tatatactct ttcggaatca caccgcctac tatcttgttt acaaattcaa aaccggttcc
      661 gggttcctta ggctcaatct caagccaaac atggccgtat tgtcctttgc cccccgactg
      721 tctgacaaac tttccttcca cctgtacttt ttttcggatg gtttctttat aagcaacctg
      781 cggcttgccg acatttgctt caaccttgta ctcccgcatc atcctgtcaa cgattatgtc
      841 cagatgcagc tcacccatgc ccgcaattat ggtctggccg gtctcagcat cagtatatgt
      901 cctgaatgtc gggtcttctt ccgccagttt ctgcagggct atacccatct tatcctggcc
      961 tgcctttgtt ttgggctcta tggcgacatt gataaccggt tccggaaaat ccatcgattc
     1021 cagtataact gcattttcct catcgcaaag ggtgtctccc gtagtcgtat ccttaagccc
     1081 cacaattgct gcgatatcgc ctgtataaac ctcttttatc tcttccctgt ggttggcatg
     1141 cagctgcact attcttccga ttctttctct tttgcccttt gttgaattat atacatagga
     1201 tccggcttca accatccccg agtatactct gaagaaggca agcttaccca cataagagtc
     1261 gaccattatt ttgaatgcaa gagctgagaa gggttccttg tcatcagaat gcctgacaat
     1321 cttctcttct ccatctacag tagttccttc aatgggcgga atatcaatag gagaaggcat
     1381 aaaatccaca acagcatcca gcataagctg tacaccttta ttcctgtacg aggagccgca
     1441 aacaaccgga ttaatcttga cactgattgt accggcccta atggcatcct tgatctcctg
     1501 ctccgtcggt tcttctccct ccagatactt catcatgaga tcctcattca tttcggatac
     1561 agcttcaatt agcttctccc tgtattcttt tgccttatcc atcatgtctg aaggtatttc
     1621 ccctacctcc atatttttgc caaggtcgtc cttgtaaata tgagctttca tacctatcaa
     1681 gtccactata cctacaaatc tgtcttcgct gcctatcgga agctgtatag gaacggcatt
     1741 ggctccgagc ctttctttca tcatttcgac tactctgtag aaatccgctc ccataatgtc
     1801 cattttatta atatatgcaa tccgcggaac attgtatttg tctgcctgcc tccagacggt
     1861 ttccgactgg ggctcaactc cgcccttggc gcaaaaaact gctacagctc catccaatac
     1921 gcgcagtgac ctttctactt caacagtaaa gtccacgtgc cccggcgtat ctattatgtt
     1981 aatcttatga tccttccact cagccgttgt agctgcagaa gttattgtta ttcctctttc
     2041 ctgctcctgc tccatccagt ccattgtggc agttccttcg tgaacttcac ctaatttata
     2101 ggtttttcct gtataataca gtactctttc agtcgttgtc gtctttcctg catcaatatg
     2161 ggccattatt cctatgtttc tggtcttttc caacgaaaat tgcctaggca caattatcct
     2221 cctttctgtt gcaagtatca ctgttatact tcccgaaaat tttaatattt agcattaatt
     2281 tataatcatt tttataagtc acaattacct cttttaagat taccacctgt aatgtgcaaa
     2341 ggccttattg gcttctgcca tcttatgagt gtcttctctc ttcttcacgc tgctgccggt
     2401 attgttggca gcatctaaaa gttcaccggc caatctttct ttcatggtct tttcccctct
     2461 ttttcgggca tactcgatca gccacctcat ccctaatgct tgccttctgt caggccttac
     2521 ttctatagga acctggtaag ttgctccgcc tattcttctg gctttgactt ccagaacagg
     2581 catgatattg ttcatagctg tctcaaatac ttcaaggggg tctttacctg tcttttccct
     2641 cagtatttcg aaggcaccat aaactattct ctgtccgaca cttttcttgc cgtcatacat
     2701 aacgttgttt ataagcttgg taactatctt atcgttgtat aacggatccg gcaatacgtc
     2761 cctttttgat atatgtcctc ttcttggcac tatacttccc tccttaatat tattttaata
     2821 tcatcggtac tcggcagtat caagccaacc aactgccgtt gtgcgcaaat atattcaaaa
     2881 caaaggctgc agtcagccaa tgtcaaataa ctcgcacaat attatttctt tggtctcttg
     2941 gcaccgtact ttgatctgct ctgctttctg tcagcgacgc cagcggcatc aagagtacct
     3001 cttactatgt ggtaccttac gccgggaagg tccttaactc tgccgcccct gataagaaca
     3061 accgagtgtt cctgcaggtt atggccgatt ccggggatgt agcctgatac ttcaatgttg
     3121 tttgaaagtc taaccctggc aatttttctc aatgcggaat taggcttctt gggtgttgta
     3181 gtctttacta cagtacatac tccccttttc tgcggacttt ccttcaatgc tggtgcagtt
     3241 gatttgtact ctaccttctc tctgcctttc ctcactaact ggctaattgt aggcattaaa
     3301 tgcacctcct tgttttattt atcatataca ggcccggtat ttgccaagct ctgctatacg
     3361 cggtgaaaac caatacacta aaaaacctct tcatatgaag aggtctgata tgcatactaa
     3421 tccttgatag ccgccgtcga ggcgccgata tcaatgccgc atgcatctcc aagctctttc
     3481 atattgctga catatatgat ttgtatatct ctatctctac atacatccag taccgattgc
     3541 ttaataaagt catcggcatc ctctgccagg taaacctttt ttaccgtccc gctttttatt
     3601 gcctttactg tctgcttcaa ccccactgtt cttctgtgta tggccttcag ttcctgcatt
     3661 caaaccacct cttaaggatt tttttgcatg gcagcatcag acatcaacaa tcaaattata
     3721 aatactgtat tcaactgcaa gcttagaaaa ccattattaa tggttttcta taaaacacta
     3781 gattagttta gcatttgtta taacatgatg tcaataatgg ttttttgcat ttgcgcaaaa
     3841 aacttttccc gcctcatatt actcggcttc cacctgtttt attgatatat ccatatattt
     3901 tttcataccg gtgcctgcag gtataagctt tccgattatt acattttctt tcaggcccac
     3961 gaggggatct atcttaccct ttattgcagc ctcggtaaga acccttgttg tttcctggaa
     4021 tgatgcggct gacaagaagg aatcggttgc cagggaggcc ttggttatac caaggagtac
     4081 tctcttcccc gtagccatct ctccgccttc ggcacttacc ctttggtttt cttcttcata
     4141 tttaaatatg tcaaagagcc ctcccggaag cagatcggta tctccggatt cttctatctt
     4201 gacttttctc agcatttgtc tgacaatgac ttctatatgc ttatccgcaa tatcaacacc
     4261 ctgaagcctg tataccctct gaacttcctg aagcagatag gattgcaccc cggctactcc
     4321 tttgattttc aatatatcat gaggattaac cgaaccttcg gttatttcat ctcctgcttc
     4381 aattacatct ccgtcggcaa ccctaagcct tgagccgtat ggtattgtat atgtcctgga
     4441 atcgccgtct tcagccagca cgacaacatc tttctttttc ctctggtcta ttatttttac
     4501 aacaccggag atttcggata ttattgcaag ccccttaggc ttcctggctt caaaaagctc
     4561 ttcgactctg ggaagaccct gggttatatc atcaccggca acgcctcccg tatggaaggt
     4621 tctcattgta agctgcgtac cgggttcccc gattgactga gccgcaatta ttcccactgc
     4681 ctcgccgata tgcacgggat ctcctgttgc caggtttacc ccatagcatt tggtgcatac
     4741 tccatatttt gaatgacatg tcagtacaga ccttatcgga actttcttaa tccccagctt
     4801 gacaatacta tttgccttct tttcggttat aagctcattc ctcttaacca gtacctcccc
     4861 tgtctcagga tgcagtatat cgctgctggc gtatctgccc ataagcctgt ctttcagctt
     4921 ttcaattact tcatttccgt ctttgatttc gctgaccatc aaaccgtctt ccgtaccgca
     4981 gtcctcttct ctggctatta catcctggct tacgtcaaca aggcgtcttg tcagatagcc
     5041 ggagtcggca gtacgcagcg cagtatctgc caagcctttt cttgcaccgt gggttgacgt
     5101 gaagaattcc agtacatcaa ggccttcacg gaaattggcc tttataggaa tctctatgat
     5161 tctgcctgag gtatccgcca tcaggcctct catgccgcac aactgcttga tctggtttac
     5221 gctgcccctt gcgcctgagt tagccatcat atatacggga ttaagcctct ccaggttatc
     5281 catcaatgct tttgttacat cttcagttgc tattttccag gtctctatta cctgctcata
     5341 tctttcatcg ttggatataa ggcctcttct gtacattttt tctattttgt ctactctctc
     5401 atctgctttt gaaagcagct ccttcttggc catcggtata tccatatcag atatgcttat
     5461 tgttattgca ccctttgttg agtaatgaaa gcccagtctc ttaatttcgt ccagcacaat
     5521 tgcggtattt gtgttcccat gcattctgta gcatttatcc actattttac ccaggagcga
     5581 cttatccacg ggcatcaaat ttccgtcctt atcccttgga tcaagctcca gaaggaacat
     5641 atcctcttcg gtatttctct caacaaaacc aaggttctga ggaattgact gattgaatat
     5701 gaatcttccg acagtatttt caacaaacct gctgaccatt ctcccgttaa tctctttggt
     5761 tattcttacc ttaacctttg cgtgaaggga aacttcatcc aattgataag ccatgagcat
     5821 ttcgttcaaa tctgtaaatt ccttgccttc gcccttatgc cccggcacat aaatggtaag
     5881 gtagtatacc cccataacca tatcctgagt cgggctgaca accggccttc catccttggg
     5941 agccagtata ttattggcgg acagcatcag aaatcttgcc tctgcctgtg cttcaaccga
     6001 taatggtacg tgcaccgcca tctggtctcc gtcaaagtca gcgttatatg cagtacatac
     6061 caggggatgt atcttcaatg ccctgccttg tacaagcaca ggttcaaaag cctgtatccc
     6121 cagcctgtga agtgttggag cacggtttaa aagcaccgga tgctcgctta ttacctcttc
     6181 gagtatgtcc cagacctccg gtttcactct ttcaaccatt ctcttggcgc ttttgatatt
     6241 atgagcaagc ccgtcattaa caagcttctt cattacaaaa ggtttaaaca gctccaaggc
     6301 catctcatta ggtaaaccgc attggaaaag cttcagctca ggccctacaa ctataactga
     6361 acggcctgag tagtccacac gttttccgag gaggttttgc ctgaacctgc cctgctttcc
     6421 tttaagcata tcacttaagg atttaagagg cctgttgccg gggccggtta cgggccttcc
     6481 tctcctgcca ttgtcgataa gtgcgtcaac tgcttcctgg agcattctct tttcatttct
     6541 gactatgata tcgggtgctc ccaagtctaa cagcctctta agcctattgt tcctgtttat
     6601 tactcttcta taaagatcat tcaggtcgga ggtggcaaat cttccgccat ccagctgcac
     6661 catcggcctc aaatccggcg gtattacagg tatcgcctca agaatcatcc actcaggctt
     6721 gtttccggaa ttcttgaaag cctcgacaac ctccagcctt ctgactgttc taagcctttt
     6781 ctgccctgta ctgcctttaa ggtcttgcct cagttccttt gacagttttt caaggtccaa
     6841 atccagaagc agttctctta ttgattcagc tcccattcct gccctgaatc tgttgccgaa
     6901 tttctcaata ttttctctgt attccttttc acttagcagc tgcttttttg aaagaggagt
     6961 atcaccgctg tcaattataa tgtatgaagc aaagtacaga accttttcca aagccctcgg
     7021 ggacatatca agtataagcc ccattctgct gggtatccct ttaaaatacc aaatatggga
     7081 tacgggagca gcaagctcta tatgccccat cctttctctt cttaccttag ccttggttat
     7141 ttcaacaccg cagcggtcgc atactattcc tttgtacctg attctcttgt atttcccgca
     7201 gtggcattcc caatcctttg taggaccgaa aatcttctcg caaaaaagtc cttccttctc
     7261 aggcttcagt gtcctgtagt ttattgtctc aggcttcttt acctctcctc ttgaccactc
     7321 tcttattttc tccggggatg ccaaaccaat ttttattgct tcaaagttat ttaattcaaa
     7381 caaggagtta tctctccctt ctaaaaattt tagaactctt cgccatcctc ttcaacttct
     7441 tcttcaactt cttcttcaac ttcattatca cctatgagaa ggtcatccag gtctatctct
     7501 gcatcaaagt cttccgctcc ttcggattcc atctcataat cctcgtaaag atcctccgga
     7561 gtgaccgtct cccctttata tgaaagactc gagaaatcca tgtcttccct tacttccata
     7621 tcagcgccca aatcctccac atcttcttcc accgattctc ttatggtaat ctcttcatgc
     7681 tcctcggtca gaaccttgac atcaagggcc aggctctgca gttcttttat aagaaccttg
     7741 aaagattcag gcacgccggg ctcgggtatg ttttcgccct tgacaacgga ttcataggtc
     7801 tttactcttc ctactacatc gtcagactta acagtaagta tttcctggag tgtatgggct
     7861 gaaccatagg cttccagtgc ccacacttcc atttcaccga acctctgacc gccgaactgg
     7921 gctttacccc caagaggctg ctgcgttaca agagagtatg ggcctgtgga ccttgcatga
     7981 atcttgtcat ctacaagatg cagcagcttc agtatataca tatagcccac tgtaatatca
     8041 ttgtcgaagt actctccggt acgcccgtcc ctcagtgtag cttttcctgt atgattgagt
     8101 tcaattccgt caataggagt ttcaccaaat gccttgagta ttttctcaac ggactttaat
     8161 tccgtaagat ctgaagactt tatcatttcc cttaaggagg tgaacttgct gtcaatgtca
     8221 tagatttcat gatttaacag ttcgtaaatt atttcaacat tttcttttat ttcagacctg
     8281 atatccgttt cagcaagctg ctgcccctca taatcttcat cattctgctt gtcggcaata
     8341 agaattctct cttttattat tctcaggttt ttctttacct gaagcacctt attggcgcat
     8401 tcgattgtct ctaagatact ttcctccgtt gccccgtcga ataccggagt agcaattttc
     8461 cagcccagtg ctttagccgc aagtgcaaga tgcacctcaa ggacctggcc gatattcata
     8521 cgtgaaggca cacccagagg attcaatact atctcaagag gtgtcccgtt gggcataaag
     8581 ggcatatcct cctccggaag cactctcgat ataacaccct tgttcccgtg gcgccctgcc
     8641 atcttgtcac caacggatat cttcctcttt tgtgctatat aacatcttac cagttcgttc
     8701 actcccggag gaagttcatc tccgttttct ctggtaaaca cttttacgtc tacgattatc
     8761 cctgattccc cgtgaggaac tctgagcgaa gtatctctta cctcccttgc cttctcaccg
     8821 aatatcgccc ttaagagcct ttcctcggca gtaagctcgg tttctccttt cggagtcact
     8881 ttacctacca ttatatctcc ggctcttact tcggcgccta ttcttatgat gcccctttca
     8941 tccagatcct tcaaggcttc ttccccgaca ttcggtatgt cccttgttat ttcctcgggg
     9001 ccaagctttg tatcccttgc ttccgcctcg tattcctcta tgtgtatgga ggtgaaggta
     9061 tcctctttta caagcttttc actgataagt atagcatcct cgtaattgta gccctcccaa
     9121 gtcatgaaac caatgagcac atttcttccc aatgctattt cacccatatc ggtagaaggc
     9181 ccgtcagcta ttatgtcgcc ttttttcagc acttcgccct ttcgtgctac aggcctctga
     9241 ttgatacagg ttccctgatt ggagcgcagg aacttgagaa gcttgtatcg ctccttttca
     9301 cctaagctgt tccgcataac tatttcattg gcgctgacct tttcaaccac gcaatcgctg
     9361 tttgccaatc ctatgacacc cgaatccctg gcagatttgt attccatgcc tgtacctact
     9421 ataggcgaat cgggctttat tataggaact gcctgacgct gcatgttcga acccatcaag
     9481 gctctattgg catcatcatt ttccagaaag ggtatcatag cagttgcaac ggatactacc
     9541 tgctttgggg atacgtccat atattcaatc tccatagtag gtacaataac tatttcattg
     9601 gcatcccttg ccgtaaccct tctgttaacg aatcttcctt cttcatccag aggttcgttt
     9661 gcctgtgcaa ttacatactc gtcctcaacg tcggcagtaa gatatacaat ttcatcagtt
     9721 acatattttt caccattatc atcagtcttg acagttctgt acggtgtttc gataaatccg
     9781 tactcatcta ccttggcata agtactcaaa gaaccgatca ggcctatatt aggaccttca
     9841 ggggtctcta tggggcacat tctgccgtag tgggagtggt gaacgtctct gacttcaaac
     9901 cctgctcttt ctcttgacaa acctccgggt ccgagagctg acagtcttct cttatgcgta
     9961 agctcagcaa ggggatttgt ctggtccatg aactgtgata actgggaact tccaaaaaat
    10021 tccttgattg ctgcggctac aggccttata ttaaccagct gctgcggtgt gacaacatca
    10081 atatcctgga tagtcattct ttcctttaca actctctcca tcctggataa gcctattctg
    10141 aactggttct gcagcagctc tcccaccgac ctgagtcttc tgttgcccag atggtctata
    10201 tcgtctttgt tcccgatacc gttagaaagg tttatattat aattaataga agcgactata
    10261 tcatcaatca gaatatgctt cggtataagc tccttaatcc ggttcctgat ctcttccttg
    10321 atttcgctct cagaacctgc cttttgcagt atatccttca gcactgataa attgaccaat
    10381 tcctttatat taagatctga tacatcaaag ctgaggtatt tgtttatatc cacagtattg
    10441 ttccccagta tcttaaccgt ctttccctcg gctgtgacat atacttcgtt tatacctgca
    10501 ttctgaattt tcacagcttt atccctggtg attttctccc cttcttccgc cagaatctct
    10561 cctgtcctgg gatccgccaa ctctctgtcc agcttttgcc cggtcagcct gctgctcagg
    10621 cttagctttt tattgaattt atatcttccg aatttggcaa ggtcatatct ttttgcatca
    10681 aaaaacaagg cttcaagcag ggattttgcg ctttctacag tcggtggctc tcccggcctc
    10741 aggcgcttat acacttctat taagccttct tcctcattct ttgtcccatc cttttgcagg
    10801 gttgctttta tcctatcatc ttcccccaat attccatcaa tctgttcatc ggtgccaaag
    10861 cctaaagctc tcaaaaaaac agttataggg atttttcggg ttctgtcgat tcttacccaa
    10921 aggatatcat tggaatccgt ctcatattcc agccatgcac ctctattagg tataacagtg
    10981 cttgaataca gcttcttccc ggacttgtcg atggtctctg cataataaac ccccggtgac
    11041 cttaccagct gacttactat aaccctttct gcgccattga taataaaagt acccgtgtct
    11101 gtcatcaaag gaaaatcgcc catgaaaact tcctgttcct tgacctcatt ggtttcctta
    11161 ttgatgagtc tgatcttaac ttttaaagga gcagagtatg taacgtccct ttccttgcat
    11221 tcctcaactg aatacttcgg ttttccgtct aacgaataat caacaaattc cagaatcaga
    11281 ttacctgtgt aatcctgaat aggtgaaaca tcattgaaaa cctctctaag accctcctcc
    11341 agaaaccagt tataggagtt tttctgcact tcgatcaggt taggcatatc cattacttcg
    11401 tcgatttttg agtagcttat cctctgattt cttccggcag agattggatg taccagcata
    11461 aagtccttca ccccttaaat ttaaataaat taaaataccc aaaagcaaaa aaattcccaa
    11521 aaagtaacgc ttttggtttg tccgtacagt taaagcacac ctattttaat attgcctttt
    11581 ttaattatac ttattcaaat gcagcttcaa atgcagcagt atagcgcaaa aaaacttact
    11641 acaatgcaat atataatgtt aacataccaa taaatgactg tcaagcataa actgataaaa
    11701 ttggtatgta ttttgctaat ttttctattt tctgatttgt caataggtat ttttatttcg
    11761 acagcgaata caatgcaccc agaatagcag cagtatatga agctgccgac aatagtgcta
    11821 ttgaagctat ctcaagcttg aaaactgctt tctgccttgc tttgtttact gccagcatat
    11881 ttttcaccga ttcaagctcc ttctgtatat cctctactct ctgatacctg tcctcaggct
    11941 tgttctccat gcatttggaa attatcctgc cgacattaga tgctgccctg ctcttcctgc
    12001 gcctcagact gtaatcggca cttccgggaa gcctgccata cagcaaatga tatatggtta
    12061 cccccatgtt atatatatcg gttctctgtt ctgtctttcc cccatttatc aactgctccg
    12121 gtgccgcgta tgcacttgta cctgcaaaga aggtatctcc ggattcatac tcgcagtact
    12181 cctgagatat cccaaagtca accagcacca gtctgacccc ctgtgcaagt ataatatttg
    12241 acggcttcat atccctatat ataataggcc tcggcttcat gccgtgcagg tatttcagta
    12301 tatcacacag ctccagaaac cagtctgtta ctgtctcgag atcaaaactg ccgtgccttt
    12361 taagcagttt gtccaaggag gaaccctcta tattgctttc tacaatataa ataccctgct
    12421 cattgcggaa tgcgtcaaca acccttggca ggcttgggtg gtataacctt ttcagtatct
    12481 caatctccga caaggtttta tcatttatct tgtccggcgg aatatgcttt acagcccatt
    12541 tgtttccgat ctcaatattg gtgcataaat agacagagcc tgtccctcct ttacccagaa
    12601 gcctttctat tcggtacttt ccatttaaca cgtctccgat tatcatcttt caccctccat
    12661 tgcacagtgc cgtcattcca gtggcagcgt atcatcacag ggggctgctg cagtagttgc
    12721 atcttcaata ccttccgccc cggtcagcct gaaggttatc acttcttcac agtccgggtt
    12781 ctttcggcag ctcttatttc tatgtcctct gattatgtgc agggtaagtg caaggtttag
    12841 aataagagta aaaactccga ttgatatgta aatcatatga agtttcataa ggttacctcc
    12901 gacatgtcct gattttacag ttcttcctgc atcttgaaaa catattcgta atttgaaaac
    12961 tttattctat cattatcctt tattccgtat tccctgttgc tgggaatttt tacgccattt
    13021 acaaaagttc cattttttga gtttagatct cttatgtaat aaaacccttc gttaaaggta
    13081 atttctgcat gaagctttcc tattgtacct ccctgtatga tatggtctgc catactgcct
    13141 aatcttccta ttataaaacc atccttgttt ataatcaccc tttcaaccct attgggccct
    13201 atgctttcaa gataaggacg gcaatcctta ggaatttcag aaatgcaaac ggtgtcacag
    13261 gcttttacta catccggcga tgaaaacacc ggtttatacc tgctcctaag gacttcatca
    13321 tggcggcctg cacttctttt tactttatct tgtgctatac gctgagtatc atctgttgcc
    13381 gctctcttac cccggctgcc cgatatccta ttaattacga gaatatccag tgcagctgaa
    13441 ataatcacca cccctgcaat ggatacaata tcgcccattt tccgggatat caaaaatagg
    13501 catgcggcag ctgcagctac taaaaaaatc agctgcagca gcatgatgct tcggatattg
    13561 ctgctttttc ccgcatcttc atatgccctt tccttcgggc ttctgctgct gggggtgctt
    13621 atgccggcag ctgcttcttg aacggctgcc ccctgtccat attcatataa ttctccgctg
    13681 ctcctcaaat ccactatcaa cctgttaaaa tcaatgagac tgaatgtatc tgattttaaa
    13741 taattcagta ttctctgcag ataattatcc cttgcattat catctgcatt ggatgagtta
    13801 accacaatat ccttaaaaaa ggatcttaat atctccgtta cattcctatt gcaggaaaca
    13861 ggtacatata acagcgaagc ttccgccgtc gcaggattaa taaaaatata atccggatgg
    13921 atgatgaatc cggaaagatc caataagtaa ttaccgctaa gcatcaggtt ttttgcaatg
    13981 cttctgagca gatccaaaag ccccgccctg ttaaggcttt ttctttcgat gtaatgggat
    14041 aatgatattt tggaggttat attataatag ataaaggtgt tttcattttc ccgccttaca
    14101 tggaatggca taaaagcaga attcggattc tgacatagta tctctacctg atgcttcaac
    14161 agtgcagatt cactgtcaat cttaagaacc aaataactgc cggcagcatt attatcatag
    14221 ctgtattcaa aatccaatac tgtatttctc acatcaacta cctcccggtt taattaaggt
    14281 attattactg ccagcactcc tgccagtata aagggcgcaa aaggtatttc gcttttcttg
    14341 ttgtcaggat tcaggcatat taatactatg ctgaacaagc cgcttagaat tgcagaaatc
    14401 agcattgcgg atattattct ttcaaagccc aggaatattc ctgaacagcc aaagagtttt
    14461 acatctccga gtccaaggcc gcccttagta atataatgga taagaagcag caccaatcct
    14521 gctgtcacac cgcccaaaat gctgtttaaa aattctcttt gggggtcaat caacacaaag
    14581 gccattccgg ctattgtacc tgcaagcacc agcttatccg gtatcctctt ctttctgata
    14641 tcaataattg ctgctgcagc aagtaatatg ataattgtta aatatggaag ataatgactc
    14701 ataataccct cctaatgagt atttgcccta tcccagcggt ttgcatacct tgcacggagt
    14761 atagcccgat ccttcggctt cttcaattaa tattggaatc ctgctttttc taagatatcg
    14821 gcagtcccct tcatggaagc ggatacctgt ccttgtaata tatacgatac tgccttccgg
    14881 ctccaggtcc aaaccttcat cctcggctgt cagtattccg aagctgccgt tgatccacgc
    14941 cctttccaca gctgtatgaa tcattcttat attgaaaggc tcaataaaag gaagtctcac
    15001 caaatattcg tactccaact gaagcacaac gtcatccctg ctgaaatcct tatccggagt
    15061 caacccaagt tcactatata ttgctctttg tgaccttgcg ttatattctt cctcgccctg
    15121 aggaaactca acaagccgca gccttatatt ctcccgatta atcagcgggc ttttcaggtg
    15181 ttccttgata aacgcttcgg caatataata tttggcatca gacttcatgt cccaatatgc
    15241 aggtgttatt ctaccgacaa catttcctat tgcgccttta ccgtaatcct ttaatatgtc
    15301 ctgcaccgct tcagtgaggt ctgttccttc aagctccccc gatacaatag ctcgaaacag
    15361 gttatcagag ctgcctcccg aaatttcccc agccagcttt tccagttcct cttcaagagt
    15421 aggtattttt gcattcccat actctcgatg cttttcatcc tccagttcat taatgaagga
    15481 tatgggatat gcggctgcag ctatttcctt tgctgcttca tttacaacat gatcaagcac
    15541 catcccggta catgtaaact tgataagaaa aagcagcagg aaaaaaacgc ttatgaaaac
    15601 cggaaataca gcacaagcct ctactgttat tgaacctctg gaacgtagtt ttttataacc
    15661 tttatacaat ttcaaccacc ctaatagcct acatatattt gcttatatat taaatacttt
    15721 ccgttttcaa aactgccggg cagcagcctg tcaaccttca gcaggggaag aaaaaaaaga
    15781 tttaccttcg ccttaacaga ggcagatatg acggtgctgt aatctcccag cctgaaatcc
    15841 gaccttggaa gctctgtctt tgcatccaca acctgcttaa tgtttacttg aataagttgt
    15901 cttgcccttc ttaaaatttc ttcctccggc ttcattagaa gcagtatctt tagatgatcc
    15961 gaatacttta atctaactgt tgtaaagctc ttcgggcata ttggcacagc ctcgccgtta
    16021 agcatattga aaatatccaa acttgagtca agggcacctt cggcaagggc aaatgccagc
    16081 ctttgaggcg gaaataccac agtgctcctt ataaaagcat ctattgtatc taatgcaaat
    16141 ctcaatgccc aaacctgaag cagcactttt gttactacat aatactcggt attttttatc
    16201 ggactgtatc cttcctcaat atctgatccg ctgatgatat actccacttc actctttctg
    16261 aagtaatgac ccctttctgt cccggaggtc aggaaggtat acttgtccat tacgtattca
    16321 atggtattta ccttttcaaa tgcgtttgat gcgacatctc caattgcttt tgcaagcttt
    16381 tccaaggttt tgattatagc atcattgctt ttctcggctt cttcttcccg cattgatgcg
    16441 ctgaaatatg tactttcgtc catttcgcca aagttctcgc cggttatcag actgctgtct
    16501 tcaaattctt cgggctctat cagccaaccc ttttccagcc tcctgaacaa tgcttcctta
    16561 atttcttttt ttgtcttgct gataaagctt ttcaattctt ccgtcttctt gtccggcagc
    16621 tgtacagcct cttccttcaa ggaatagccc tcaagagaaa tccttccaag cttcattttc
    16681 aaatctgaca tttcctcatc aatttctgat tccagccgtc ttattttatc tttaacttca
    16741 tcaatctctt ctctggtttc atctatctcc tccgcaacac cggcatcatc ctcctgaaca
    16801 tccgagagct tttcttttaa ttcatccagc tcatccttaa ggctttctag ctttccctcc
    16861 atacgctcca gctcattctg caagggctta accagcagaa gtgtcttatc tatttcctct
    16921 atatacattt gaatcccggg agtcaggttt tcattgtttt tctttatgct gtcaaactcc
    16981 cgggactgat tctctacaaa ctggccttcc tcagccttaa tgtttgtttc atccctgctt
    17041 tggatataat cagcaagaag gcctccggca ttcccattgc aaattaagct gttgatttcc
    17101 aaggctcctg acagctcatc ccttatatcc tcaagctttt ccgcgcttat atttgtcagc
    17161 ttcttcttaa tacttgtaag ttttatattt acttcactta ccttggctct cagctcattt
    17221 gccttatctc tggtaatctt ctcacttttt gcaaattcaa tccctttatt cagcttaata
    17281 ttctgcagct gctctataag gctgccggtt gctgaaatta ctgttctgta tttcatatat
    17341 tcctgtacct gtctattaaa agtctcatgc ttcaacaggt tttccatgcc ttgtatttca
    17401 atatcctcat aatctatact tatatttata aagtttatat tcttatgccg ctcctttaga
    17461 tttacattca aatatctata aaagtcctct tttacatttc cttctgcagc attcactccg
    17521 tatattccat agctgcccat aagctcactg tcatagtctg ccaatacaga cctggctgag
    17581 gtgttcagtg tactttgaac tctccggtcg gcagcgataa ttctcacaat atcaattact
    17641 gttcctgcaa aaattatcag agacatgaaa ataagcgcca gaaaaacagt aattgcccct
    17701 ttctcttttc ttatgctgcc atccctttgg tacatttgcc tctccccttt gatgaccaat
    17761 catcttaacg ttttgcccct attctgcctg ccagttcacc gaggtcaagt tcctctccaa
    17821 gcctctttga taactccagt ataaggtcaa cattccttat atactcatcc ggctctgata
    17881 ctgctgcttc ggattgaaca ctcaaagtca gagtatcctt accgtcaaag aattccttta
    17941 tgccgcctaa aggcacctta atctcctgga ttaactcaac tatgagtctt cccctaagag
    18001 cgttgttatt gaatgtaatc cttattcttg tactttcggg cttcaggatt gtgctttcaa
    18061 tccttttgca caaatcctcc ccaatcagaa gggctttatg cccgggcaga ttattccccg
    18121 catccatttt aaaaaccacc ctggttttgc cattgtcaga tgcttctctt tcaaaatatc
    18181 cttcataggt tgtacttgaa aacagcagat tgtcaaaaat tctgtagccc attgaatctt
    18241 caggtctttt attgtttatt ttcccatcct ccatatttct tctgctgtca agccataact
    18301 ccgccccctg ctgagcagca aaggaggcaa agctttcaag gcttgccctt tgccccatgt
    18361 ataagaatgt aaacaaaagt gccaataagg ccagcataat cgtcacaaac accatggagg
    18421 cttcaacagt atagcttccc ctgtcacata tatgcttgtc cagcaatcat cacctccgca
    18481 cccttttcag gaagtaaata tcctgtacaa ggcgctagtc cgtcggcaac ggtgcagtca
    18541 tgtcaatttc gttattttca acagcagggg cagtatcaat aaaatttctt ataaccctct
    18601 gcacataagc agttatcgac tttctgaaga gcaatgctat tcctataaga attgcaatta
    18661 ttaccacaat ctcaaccgta cttatgccat cctcctcaac aataaatcct tttacttcct
    18721 ttaacatttt gctgcctcct ttaattttta tttggtattc gtgtatcata tttaaagata
    18781 ccttaactgc attactgcag ggagtgttac aataataagt attcctacaa acataatcat
    18841 cagcggaagc accagcttgg ttgaggcctc ttcccccagc ttcttcgcca tattctttct
    18901 aagctgccag cattcattcg attgaagctt cagcagcggg accaaatcgc tattgccttt
    18961 tttcaggttt tgtatcacgg cagtaatgaa tttggttatt tcctttaccc tgcatcgttt
    19021 tgcaaaatcc tcataagctt ccatttcagg ctttccgttc ataatctcaa tataggttgt
    19081 tcttagctct tcatataggg gagtgatatt ttttctgtcc ctgactatct tctcccaagc
    19141 cctgggtaca gtcattccgg cattgataag gagcaccatc ttattaagaa aatcaggaaa
    19201 atcgtatcgg atgttaaaat tccttttttt gattttttca tccaactctc tgtctgaaac
    19261 ataaaaaaca atccccagtg ccgacaatgc aaatattgca tagatgatat caggcttttc
    19321 cattaatgcc cccagcagag taacagatag cagtgcaata atcatgtgaa ttaccttatg
    19381 tacaaaatgg agcttgagca tgtgctttat atccctgcct ccataaagct cagccagctt
    19441 tatgccgagc ttcctttcat aagcaatgaa cctccctgca atgagcttgt ctgttatgaa
    19501 taaagcagca ggcgccaaaa ctttgtatat atgccctttt ttaaagcctg tttcggtaaa
    19561 atcttcatat ttgcccttta cataaaagca aataaatcca agaacaataa tttccgtaat
    19621 aaataccggc agcataagct atacctcaat atccatgatt tttctcgata caaaataagc
    19681 ggctccaaaa agcactaatg aaaaagtcat tgccgctctg ccaaggggct ccgaaaatac
    19741 cggagccatg aattcctcag cgctggcgga cagcagaagc acgataagca ccggcatcag
    19801 gttaagcact ttatgttcca gccttttttc cataagcaga agatttatct cctgccttat
    19861 atcaattttc tcggttatta tttccgcagt attctttact acctggacaa gattacctcc
    19921 tgtgcgcttg catattagaa aaacatcggc aaagtttatt atatcctcca aatgcgacct
    19981 tgcagcaaag tcagccaatg cttcctctat ggtttcattc atctcaattc ttctgcttat
    20041 atgctcaagc tcgatcaata tatatgtatc attgttggga tactgtatgg aaagctcctt
    20101 aatagagccc ttgatcgcag tttctattga ctttccggca gacagagaag atgacaatga
    20161 gtaaagggct tctctgaact gcaagttgag ttcgtttttt ctttttttta ttatttcttt
    20221 tgtcctgaat ccgggataca aaagggcaaa gggcgttaat agcagcgata acaaagcact
    20281 tctgtagaaa atataacctg tcgcaaatat aactgtaccc gccataaata tgcataaaag
    20341 cttttctttt aatgacatta tataagtatc atagtctgcg agagatgcat gtgcagcagt
    20401 accccttcca tattcgtact ccgttgagac tgccggccat tttctaagat ccataaaaaa
    20461 tcacctcata tcacttcatt caaccctgcc attctgagct ttgccaggtt tttcatagta
    20521 ttgccggttc ttacaagaca cccttcaacc ttttctgaca ttgatccgcc tcgctccaca
    20581 aattcaaaga gaggattaag ccctatcatt ccttctctgc agtctgttac ctccactatt
    20641 tccactactt ttctcgtgct gtccctgaat cttgaaaggt gtatcataat atcaacggct
    20701 gaagcaatct gctgcctaat agcctccaaa ggcatcattg cgccgctcag caccatggtt
    20761 tcaagccgtg agagcatatc agcggtagaa tttccatggc ctgttgaaat tgaaccgtca
    20821 tgaccggtat tcattgcctg aagcatatca aaggcttcag ctcctcttac ttcaccaatg
    20881 atgattctct caggcctcat cctcaatgag gcccttatca gatcccttat ggttatttct
    20941 cctttaccct cgatgtttgc atttctggtt tccagcttga ctatgttctt tatattcttt
    21001 atttgcagct cagccggatc ctcaatggtg acaattcttt catcccgggg aatgaaatct
    21061 gaaagggcgt tgaggaaaga agttttgcct gatcccgtac ccccgcatat gaaaatgtta
    21121 tatttggcct ttaccaacct ttccaggact tcggcagcct cttccgtaat agagccccat
    21181 tcaatcaagt ccttcattga tatgggcctt tcagaaaact tccttattgt tagtataggc
    21241 ccgttcaatg caataggggg aagtaccaca tttactcttg atccgtccgc caatctggca
    21301 tccgctatcg gcgaagcttc atttacgact ctgtttacct tcgaaacaac tgattgtatg
    21361 acgtcctcga gcttctgccg gctttcaaag ctgctttcaa tactgtatgt ccttccgtgt
    21421 ttttcaataa aaatattatc ctcaccgttt accattatct cggttatgga cttgtcatca
    21481 ataagaggct gcagtatatc aagcttccgt atggaattga aaagcacgga tataagttcc
    21541 tgcttttccc taatgctcaa agaccgcagc ctggcctcct caaaaacctt atctgttatc
    21601 agttcattta tatcttcatc agtgacttgg cttttaatgt ccagactttc tcttatttct
    21661 tttctcaaat tatttatcac caagtttttc gcatcatcat ccatgtactc cacctcaacg
    21721 gccagttata acaagatttc aatgagggtt tttgcatact tgcaaaaacc tacccgagcg
    21781 tttcaacgag gcgagatatg ctcactgcct gtggggcaac gaaaggggtt cccgtcactt
    21841 gtagaagtgg taacatcctt tcgcctcgtt ataatatcaa taatccggtt aacggctttc
    21901 ctgaaatcat cattttcaat aaaaatcctc ccatcctctc tgatcagtgt ccttgagtat
    21961 tcaggaatct taactgctgc agcggcattt tctccaaagc tttcgaagtc ttcatcccct
    22021 ttgtttttat acttgtttat cactgtaatg aatttatcgg aaatgcttcc accgtccgta
    22081 tcccgcagct tggcaagctc attcagcaat actctttctt tatacgatgc aattggctcc
    22141 cgcaaagtaa caagtacaat acggtcacat aatttcatta tcttgtaatt ttttgagtca
    22201 aaattgctgg acatatcgat aaaaacatag tcacagcatc ccatgccttt tatccctttt
    22261 ataagctgtt ccaattcctc cggatttatt tcctcgtatt ccatagggct atcaggagga
    22321 ttgaaatatt ccaccccatc atcagtactt tttatcccct ccattttaaa ggagaggttc
    22381 cggctttttt ctttgatata gtaataaaca taagaaagat ttcttttgct gcctaaaccg
    22441 aaaaacattc cggtggattg aatggactct agattcaaat aaaatgattt caagcccagt
    22501 tcggcacatt gtgttgataa ggatgtcgct attgtagttt ttcctgtacc ccctgcagga
    22561 gaatatacac ctatgaactc agtatccttt gaacaggatg aaagcctaat ctcttgggga
    22621 ttggccctgg agtacaaata cagcaaatcc cgaatcagct tgtctccggt actgtacttt
    22681 ctgatggcct gaaacccggg atactccctg ttcaaagctt cccctgaaag tattacctta
    22741 agcttaatat tcggatattc aaccgaaata tcataaaaat cggaactgat tgccagaata
    22801 tcggtttgag gctgcttctc aaaatatccg gcaaaagaat caagctttgt aaagcagctg
    22861 accatgaagg atgaggaatg atttgaattg atatattcaa acagccccct tgcataactt
    22921 tcgtcgcaat ctgcgataac cagattcaat cttgccatac tcaaactccc ctcattatta
    22981 tggctgccgc agttcctgat gctatagcta ttgaaaaatg aaacttccct ccgtcctgcc
    23041 tattttttaa gtcggtatat tgaagaatat ccatagtcag aaaacaactt ttgaggtaca
    23101 caaaaagatg attgaatctt tctgaagcat tttcccttgt cagcattagg cctgaagcaa
    23161 taaaacctcc aaagataaat gaatatattg cggcatataa ggcaaatgct gcgcccatta
    23221 ccgctcctac agcgcataaa agctttatat cccctgctcc tacaatcctt gtaatatata
    23281 agacaaaaag gcatgcggcg ggaattataa tgccctgcag tgaaaatacc aggcccccaa
    23341 taccttctat tgcaagattt actgccagtc ctatcagcat gaagccgtaa gtaatgtaat
    23401 ttttaacact gtaagtcctg aaatcagata ctaatgcaag cagcataagc attgaaacta
    23461 taatcagctt aaatactatt gcaatcactc ccaattgcat tgtataacat attacatttt
    23521 atacaaattt tatatgattt tgttaaatta tagcatatac tagaaatttt tgtatatata
    23581 tgatatattt ggtatgtcct attatacgaa tgaataatac agtctattta ctgtattttt
    23641 acatccccca ctgtgcagga aaatattttt cctgcataat aattgcaaat aaaaaagccg
    23701 caataa
//
LOCUS       DAOOAB010000040        23268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_2866_length_23268_cov_15.848619, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000040 DAOOAB010000000
VERSION     DAOOAB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 23268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23268
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2866_length_23268_cov_15.848619"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1248
                     /locus_tag="PKU88_09055"
     CDS             184..1248
                     /locus_tag="PKU88_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX69461.1"
                     /translation="MLKKGLILVLAIVMVMTMFTGCAQPAQAPAEPAQEAEQAPAEEA
                     KPELSFGMITDVGGLGDKSFNDSAWEGLQKAGKELGVEPVILQSTKQEDYEANIGNMA
                     QSVDLSIAVGFLMEQAMISGAEQYPDMKFGIIDMVVPKPNVMSVTFKEHDGSFLVGVI
                     AGLTTKSNIVGFVGGMEGPLIEKFEVGFRAGVASVNPDAKVLVNYTGAFDNPDKGKEI
                     AISQFKQKADVIYHASGACGIGVIQAADENGFWAIGVDQDQSAVSKNNKVLCSMIKRV
                     DVGTYTIVKSYADNNFAGGKAIELGLKEDGVGFSDGGNNVPAEVKAVADKAKELVVNG
                     TIVVPSTKKELEAFKPVEIK"
     gene            1470..2990
                     /locus_tag="PKU88_09060"
     CDS             1470..2990
                     /locus_tag="PKU88_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69462.1"
                     /translation="MPAVIEMKGISKSFPGIRANDNVNLKIEQGEIHVLLGENGAGKS
                     TLMNILYGLYQPDEGQIFIKGKPVKIVNPNVAISHGIGMVHQHFMLVQPFTVTENIIL
                     GKEPKKGAALDIDKAIKDVEEISKKYELKVDPRAVVQDISVGMQQRVEILKTLYRGAE
                     ILILDEPTAVLTPQEIEELCIILRSLVDQGKSIILITHKLKEVMSMSDRVTIIRRGKV
                     IDTLNTKDTNIDELAEKMVGRKVSLTVEKKEAEVSAPILEIKDLRVLNNRKLEAVKGI
                     DLQVHGGEILGIAGVDGNGQSELVQAITGLSKAEAGEILLAGKDITNLTPKEVIASGV
                     GHIPEDRHKRGLVLAYSLAENSILGSHHMKQFRQGFGLNYQKIREYCRRLIKEYDVRT
                     PNERVLAKSLSGGNQQKLIVAREIDRDPKLCIASQPTRGLDVGAIEFVHKKLIELRDE
                     GKAVLLVSLELDEIMDLSDRIAVMYDGKIVGILNAKDATEKKLGIMMAGGSAQDAV"
     gene            3003..4115
                     /locus_tag="PKU88_09065"
     CDS             3003..4115
                     /locus_tag="PKU88_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69463.1"
                     /translation="MESGINEFKEGLLNTLKEIAYPLVAILISVIAGGILVYSFGSDP
                     IIAYTAMIKGSLGDWNKTGETLVTVTPLILTGLSIAFAFKCNLFNIGVEGQFIIGSIT
                     AVWAGWAFAGLPAILHLPLTLLAGTVAGGIWASVIGWLKAKVGSHEVINGIMMNYIAM
                     YTSNFLVRTVLNVPGKAYSIEIADTAKLWRFSQAFEQFNHSRLHIGIILSLIVALIAY
                     YILNKTVRGYEIRAVGFNPHAAEYGGISVRKNIILAMIVSGLFAGLAGSIMATGVQYR
                     VNNLFGFTGYGLDGIAVSLVGNNHPIGIILSAFLFGVLQKGGPLMQIQGIPKEVVGII
                     QGIIILFVAANFVKVVMENIKLNKDLKRTAVKEEVE"
     gene            4117..5007
                     /locus_tag="PKU88_09070"
     CDS             4117..5007
                     /locus_tag="PKU88_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69464.1"
                     /translation="MEGVGILTIIGIVVTASIRSATPLIFASLGGNFSERSGVFNIGL
                     EGIMTVGAFSAVFFSYKTGSPWAGLLGAMVVGGLFGLLLAFMSIYFKANQVIVGTAIN
                     ILAASLTTFLLVEIWGRPGQTDNVEYFKAVGPFNLFTYLAFVMVIVSYYVMYKTPFGL
                     RVRAVGEHPRAADTLGVNVYVIRYICVMISGVLAGIGGASLSIGSMSLFKEGMIAGKG
                     FIALAALIFGKWNPIGAALACLFFGFAVALETVATNLPVPREFLYALPYIMTMFAVSG
                     FVGRAVGPAAGGIPYEKGEK"
     gene            5182..6585
                     /gene="rpoN"
                     /locus_tag="PKU88_09075"
     CDS             5182..6585
                     /gene="rpoN"
                     /locus_tag="PKU88_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPX69465.1"
                     /translation="MRMGYDLLIEQQQKLIMTPELKLALKILQLPAVELEELLQQELE
                     DNPILELADDNRDEKSEVQQKQDKKEKEKEKEIDWKEYFQFQGKSYAEEGFDSDEAAE
                     LSYENFITYSYTLKDHLISQLRVSQISKKLRDIGEYIIESLDENGYLTITKEDLAAIL
                     DVKPEEVEEALSVIQTFEPFGVGASNLKECLLIQLRNKDIYDKKIEVIIINHLDDIAS
                     NRINNISKKLSISIEEAQKYSDIIKNLEPKPGRAFEEGTSTKYVTPDVYIEKVGKEYV
                     VIINDYYGSKLMINQFYKKMLISEDKSSQASAFISNKLSSAMWLIKSLEHRKNTLYNV
                     VKAILNYQMDFFEKGPMFLKTMTLKKIAEMVNVHESTVSRAINGKYVQTPRGIFEIKY
                     FFKSGVDNQDGEAISSESIKKMIKSYVSGEDASKPLSDQHIADLLVKEGFMISRRTVA
                     KYRDELGILSSSKRKRY"
     gene            6760..7791
                     /locus_tag="PKU88_09080"
     CDS             6760..7791
                     /locus_tag="PKU88_09080"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HPX69466.1"
                     /translation="MNEFLNLQKRLVPEIIEITEKRYSILRAIEFNQPVGRRALAGIL
                     GQSERWVRSELDILKSQNLIYVQALGMVVTDEGNEVINKLRDYISELKGLKDLEKAVR
                     EMLNIRELIAVPGDTENELQVVVEMAKAAFEYIKGIIEDNDIIAVSGGYSALSVAENA
                     RRIDAQNITIVPARGGIKTDMERQANIVAEKLAKNIGGNYYLLHLPDNINEDMLKRMA
                     DDPDIAKVLNYIAETDVLIYGLSSFESISRKRNFTEEQVEELKTLNVRAEAVGCYFDS
                     NGNIVYKSGSIGIKPEHLDSIKTVMSVGGGARKAEAIVLTSKGRKNRVVVTDEAAARE
                     IYKILKGGN"
     gene            7793..8800
                     /gene="gap"
                     /locus_tag="PKU88_09085"
     CDS             7793..8800
                     /gene="gap"
                     /locus_tag="PKU88_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPX69467.1"
                     /translation="MALRVGINGFGRIGRPVFKIASEKFGDDAEIVLINDLTDTKTLA
                     HLLKYDSTYGRYDRDVQAKEHSLVIDGKEIEICAETKPENIRWGDYGVDVVIESTGRF
                     TSKEAAMAHVAGGAKKVIISAPAKNEDITIVMGVNEDKYDPEKHVVISNASCTTNCLA
                     PVAKVLHENFGIVKGLMNTIHSYTNDQRILDLPHSDLRRSRAAALSIIPTTTGAAKAV
                     ALVLPELKGKLTGLSMRVPTPTVSVVDLVVDLERNVTVEEVNAALKRAAENELKGILE
                     YCDEPLVSIDFKKDPHSSIVDALSTMVIQDNMVKIISWYDNEWGYSNRVVDLVKYISN
                     K"
     gene            8824..10008
                     /locus_tag="PKU88_09090"
     CDS             8824..10008
                     /locus_tag="PKU88_09090"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPX69468.1"
                     /translation="MNKKSIQDIDVDGKKVLVRVDFNVPLDENGTITDDRRIREALPT
                     IRYLREHNAKVILMSHLGRPKGKFNMKYSLKPVAERLSGKLGAEVRLAGDVIGEEVSA
                     MVNSLKEGDVMLLENVRFYEEEEKNDKEFSGKLAALGDIYINDAFGTAHRAHATTAGI
                     AEYLPSAVGFLIKKELEIMGKALTAPERPFVAVLGGAKVSDKIGVIENLMDKVDTLLI
                     GGGMAYTFFGAMGYETGKSIFEADKAELAGELLKKAETKGVKLLLPVDTVVASEFSND
                     APKKTVPSAMIPKDMMGMDIGEDTRKIFSEIVKNARTVLWNGPMGVFEMPNFAKGTFE
                     LAKAMAECTGTTIIGGGDSAAAVEQLGFADKMTHISTGGGASLEFIEGKVLPGIAAIE
                     NK"
     gene            10025..10777
                     /gene="tpiA"
                     /locus_tag="PKU88_09095"
     CDS             10025..10777
                     /gene="tpiA"
                     /locus_tag="PKU88_09095"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPX69469.1"
                     /translation="MRRPVIAGNWKMNKTVNEAIKLAEELKPLVKGAAAEVAVCPAFI
                     CLNDLVKVLKGSNIRVGAQNMYHKESGAYTGEISPVFLKDIEVEYVILGHSERRQYFR
                     EEDSEINKKVIAALDHGLRPILCVGETLEQREAGVTFDVIAAQVKGCLEGLENRNTDD
                     LIIAYEPVWAIGTGKTATSQDANEVIGFIRKQLALLLGNDAADKTRILYGGSVKSSNI
                     KELMDMPEIDGALVGGASLDAQEFSRIVNFNQ"
     gene            10809..12092
                     /gene="eno"
                     /locus_tag="PKU88_09100"
     CDS             10809..12092
                     /gene="eno"
                     /locus_tag="PKU88_09100"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPX69470.1"
                     /translation="MTVITDIYAREIIDSRGNPTVEVEVELEGGALGRAAVPSGASTG
                     AFEAVELRDGDKSRYLGKGVLTAVNNVNEIIAPEIIGMNALDQVAIDQALIELDDTPN
                     KGKLGANAILGVSLAVAHAAAEALGLPLYQYVGGVNAKTLPVPMMNILNGGKHADNNV
                     DIQEFMVMPVGAESFKEALRMCAEVFHNLKSVLKAKGYNTAVGDEGGFAPNLTSNEEA
                     LQVIIEAIKKAGYEPGKDLYIAMDAAATELYKEDGKYHLDGEGVVKTSEEMVDYYENL
                     VSKYPIISLEDGLSEEDWDGWKLLTERLGKKIQLVGDDLFVTNTERLEKGIEMGVANS
                     ILIKVNQIGTLTETLDAIQMANRAGYTAVVSHRSGETEDVTIADLVVAVNAGQIKTGA
                     PSRTDRVAKYNQLMRIEEGLDTMSEYLGVNAFFNL"
     gene            12361..12591
                     /gene="secG"
                     /locus_tag="PKU88_09105"
     CDS             12361..12591
                     /gene="secG"
                     /locus_tag="PKU88_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPX69471.1"
                     /translation="MRLAIVILQYVVCLVLIVSVLLQPGRSAGLSGSIAGGAEQLFGK
                     KASKGYEALFSKLTTASAILFMVLSVVLVLVK"
     gene            12696..14660
                     /locus_tag="PKU88_09110"
     CDS             12696..14660
                     /locus_tag="PKU88_09110"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPX69472.1"
                     /translation="MDLLILAPLSGVLALVFAWFLANSIAKKDPGNDRMKEIMGFIHE
                     GAMAFLFREYKVLVVFIIVLAIIIGVFLKSWLTAICFVAGACCSILAGYFGMQVATKA
                     NARTANAAQRGQNEALGIAFRGGAVMGMCVVGLGLLGLGTLYIFIRDASIVTGFGLGA
                     SSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMG
                     ADLFESYVGSIISAITLGAIVYAGKVEGVLFPMLLAAIGIVASIIGTSFVKTDEKSNP
                     QSALSRGTYGSSIIVIVASFILSRYMFGNLNAFWAVAAGLVAGSIIGFVSEVYTSSDY
                     KYVKKIAQQSGTGSATTIISGLGVGMLSTVAPIIFICAAVLVAYDSAGIYGIALAAVG
                     MLSTTGMTVAVDAYGPIADNAGGIAEMAGLPESVREITDKLDSVGNTTAAIGKGFAIG
                     SAALTALALFVSYSEAAGLKTIDILNPKVMVGMFIGGALPFLFAALTMEAVGKAANQM
                     IEEVRRQFRETAGIMEGTTKPDYARCVDISTAAALREMIVPGLLAVIVPIATGFILGK
                     GALGGLIAGAVVTGVLLAIMMANSGGAWDNAKKYIESGKMGGKGSVAHKAAVVGDTVG
                     DPFKDTAGPSMNILIKLMTIVALVFAPLFM"
     gene            14802..15071
                     /locus_tag="PKU88_09115"
     CDS             14802..15071
                     /locus_tag="PKU88_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HPX69473.1"
                     /translation="MKTLLIYLITVNIAAFAAMGADKYRAQRNKWRISESSIFALGLI
                     GGGAGIYLGMKLFRHKTRHLEFTLGIPVIIILNAIALWYILQKLE"
     gene            15278..16060
                     /locus_tag="PKU88_09120"
     CDS             15278..16060
                     /locus_tag="PKU88_09120"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPX69474.1"
                     /translation="MNDINSKYAKEFYFKGNNTGVLLIHGFTGTPYEMRFLGEFLRDK
                     GCTVRGILLKGHGTTLKDMRRCSYRDWIHGAVEGYRSLKQECDEVFAVGFSMGGLLSL
                     YLARNYDIRGVVTLSAPIRVYSRAAALAFIERNLRNHMTGKTENREINVIGYERAPLI
                     SVHHLFKLIRYVKANLKYVKKPVLIMQSYGDRAVSPKSANIIYGSIGSKDKSIIYLHK
                     SGHVITCDKEKEQVFKEVYSFIKNKSTFIREADRKEERCSSV"
     gene            16053..18191
                     /gene="rnr"
                     /locus_tag="PKU88_09125"
     CDS             16053..18191
                     /gene="rnr"
                     /locus_tag="PKU88_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPX69475.1"
                     /translation="MFNTEKEINQEIKSKLLAFMREDAYKPLSHDELIQAFQDDSLQV
                     PELDEVLNSLLEEGSIIITRKNKYGLTEKMNMVVGRIQGNKKGFGFLIPDNKDLRDLF
                     IPAENLNGAMNDDRVIVRLTAGESGTGKREGEVVRILKRANAKIVGTFENGNGYGFVV
                     PDDQRIYQDVFVSKSDFNGAKDGYKVVVEITRWPEARRNPEGIVVEVLGHKDDVGTDI
                     ISIIRGHNLPEEFPIEAENQAGKIPELVSDADIAKRRDLRDKTIVTIDGEDAKDLDDA
                     VSLEILPDGNYMLGVHIADVSQYVFENSHLDKEALKRGTSVYLIDRVVPMLPKRLSNG
                     ICSLNPQIERLTLSCDMVIDKKGKVKEYEIYESVIKTRERMTYENVNKILAGSDPMLT
                     ERYDYLADTFRQMEELMLILNKKRRLRGSVDFEFDETKIILDEKGRPIEIKPYERGIS
                     ERIIEEFMLVCNETVAEHMYWKETPFIYRVHEDPDAEKLQAFNEFIFNFGYHLKGIAE
                     IHPKALQQLTDQIKGTKEERIINTLMLRSLKKARYTSESMGHFGLAAKYYCHFTSPIR
                     RYPDLMIHRIIKEDIHGKLTEKRLKHLHSIIESIAEQSSIRERAADEAEREVEDLKKA
                     EYMKDRIGEEYDGIISGVTSFGMFVELENTIEGLVHMSNMEDDYYQFDEMHHMLIGER
                     RRRTFRIGDSVRIKVLNADIANRTIDFSLV"
     gene            18356..18820
                     /gene="smpB"
                     /locus_tag="PKU88_09130"
     CDS             18356..18820
                     /gene="smpB"
                     /locus_tag="PKU88_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPX69476.1"
                     /translation="MAQVNIKTIAQNKKARHEYFIEDTYEAGIELSGTEVKSVRKGSV
                     NLKDSYAAIDKGQVYVYNMHISPYEQGNIFNKDPMRTRKLLLHKYEINKLIGYVQQKG
                     YTLIPLSIYLKGSLVKVQLAIAKGKELHDKRHDIAERDAKREMEREFRGKNY"
     gene            18825..19173
                     /gene="ssrA"
                     /locus_tag="PKU88_09135"
     tmRNA           18825..19173
                     /gene="ssrA"
                     /locus_tag="PKU88_09135"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19324..19476)
                     /locus_tag="PKU88_09140"
     CDS             complement(19324..19476)
                     /locus_tag="PKU88_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69477.1"
                     /translation="MKYIQGQDRSQITLFPDCIEDYVGEDNPVRVIDAFVDNLDHAEL
                     GLSFLA"
     gene            complement(19683..22799)
                     /locus_tag="PKU88_09145"
     CDS             complement(19683..22799)
                     /locus_tag="PKU88_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPX69478.1"
                     /translation="MQPTMIALLNNAALLLLLPVIYQLTSLINTRNTRFKQIVSGILI
                     ALLCIAIMSVPFKFYLGVQFDTRSILISVTALVFGPIPTIITIAAAIILRILQGGAGA
                     LPGVAVIISSAIIGLVWRLWIHSAATKLRWLNVYAMSLLVHAAMIACMNLLPYPASVN
                     VIREITLPVLLIYPAATVPIYLLLTQQQVFLQVRDQLKQSEERFRLLFDKAPLGYESL
                     DIDGNIIDVNQQWLNTSGFSRDEVIGKWFGDFIHPAYKEEFLEKFSQLEETKQIHSEF
                     EMLHKSGKSLFIAVDGNIVYEPNGDFNETFCILKDITRQKKVETDLQSSEKKYRRLYE
                     TMAQGVIYQAADGRIISANPAAEKILGIPLDQMLGMSSLDPRWRTIREDGSELSGMDH
                     PSMIALRTGKPYGPVILGVYQPMLKDYVWISVNAIPLFEDGEAAPSKVYTTFQDITAE
                     LKANQNYQLLFNEMVDAFALHEIICDDQGTPIDYRFLAVNPAFEKMTGLKAADILGKN
                     VLEILPNTESYWIETYGKVALTGESVSFENYAAATDKYFRVMAYQPAPMQFACTFSDD
                     TTQVRAEHALKESEKKYSSYIENAPYAVFVVDKKGQYVEVNNAASIITGYSREQLLEM
                     TIRDITAEESMNEAMRCFTSMLDTGKLSTELKYLHKDGSIRWWTVDAVKISENRFLGF
                     SIDITSKKQAEEELTFQSRHDYLTGLYNRRYYETELKRLDIEGQLPLSVITGDINGLK
                     LINDAFGHAEGDKIIIESAMIIQGCCRQGDILARTGGDEFGIIMPRTDSVTALEVLQN
                     IQTALIKFDSENNFEMYQHSVSLGYATKEKIEEDIMYILKTAEDHMYQRKLLEANSVH
                     STIVASIKAALFEKSHETEEHAERLVELSKAIGMALNLSQVELDKLELLATLHDIGKV
                     GIKDNILTKPGKLSESEWAEIKRHPEIGYRIAMTSPELVPIAEGILCHQEWWNGSGYP
                     KGLCGESIPLLSRIIAVVDAYDAMTNDRPYRSAMEPAAAIEIIKENAGTQFDPKIAEI
                     FADLMSKE"
BASE COUNT         7188 a         4129 c         5695 g         6256 t
ORIGIN      
        1 agcgggacag gagaaacgtc cccgtgtccc gaaacgtccc cgtgtcttgc ccgtgtcctt
       61 tttgtcacat aaaaaattag ggtggaaatt tttgttggaa aatactataa tatatttata
      121 ttgcaattgt aatacaattg taatataata aacatccaat tatattatta agggggaaga
      181 ttgatgttaa aaaagggttt aatactagta ttggcgattg tgatggttat gacaatgttt
      241 acaggctgcg cccagccggc acaggcaccg gctgaaccgg ctcaggaagc tgaacaggct
      301 ccggcagaag aagcaaaacc ggaattatcc tttggtatga taacggacgt tggcggactc
      361 ggagacaagt cctttaatga ttcggcttgg gaaggactgc aaaaggcagg aaaggaactg
      421 ggagttgaac cggttatact tcagtcaaca aagcaggaag actatgaagc taacatagga
      481 aatatggctc aatctgttga tctttccata gctgtcggtt tcctgatgga gcaggctatg
      541 atatcaggag ctgaacagta tccggatatg aagttcggaa taattgatat ggtagttcct
      601 aaaccaaatg ttatgtcagt cacattcaag gagcatgacg gctcattcct ggttggtgtt
      661 attgccggtc ttactacaaa gtccaatatt gttggttttg taggaggtat ggagggccct
      721 ctgatcgaaa aatttgaagt tggtttcaga gcaggcgttg cttctgttaa tccggatgca
      781 aaagttcttg tgaactacac aggtgctttc gacaatcctg acaagggcaa ggaaattgca
      841 atttcacagt tcaagcagaa ggctgacgtt atatatcacg catccggtgc ttgcggcata
      901 ggtgtaatac aggcagctga cgaaaatggt ttctgggcaa tcggtgttga tcaggatcag
      961 tcagcggtat caaaaaacaa taaggttctc tgcagcatga ttaagagagt tgacgtaggt
     1021 acatatacta tagttaagtc atatgctgac aataatttcg ccggaggaaa ggcaatcgag
     1081 ctgggactta aagaagacgg cgtaggcttc agtgatggtg gaaacaatgt accggcggag
     1141 gttaaggctg ttgcagacaa agctaaagag ttagttgtta acggcactat agttgttccg
     1201 tcaacaaaaa aagaattaga ggcattcaag ccggtagaaa taaagtaatc aacatcgtgt
     1261 agttacatga tgaatcaaag ctagatgcgc cgctgcatct agcttttgct tcacctttct
     1321 gataccagat gccggacacc ggatacctga ttagggcagg gctttggagt gcttcacaaa
     1381 ttcttgttta aaatgatata attaagacat gcgaaatgtt gcattttttg agaaggtttt
     1441 cttatttgat ttgagaggtg ggatataaat tgcctgcagt aattgagatg aaaggtataa
     1501 gcaaatcctt tcccggtata cgggcaaatg acaatgtcaa tctgaaaatt gagcaggggg
     1561 agatacatgt attgctgggt gagaacggcg ccggcaagtc tacgcttatg aatatacttt
     1621 acggacttta tcagcctgat gagggacaga tatttataaa gggaaagccg gtcaagatag
     1681 tcaatccgaa tgtggcgatt tctcatggta taggtatggt tcaccagcat ttcatgctcg
     1741 ttcagccctt caccgttaca gagaatatca tattgggaaa ggaaccaaag aagggagctg
     1801 ctttggatat tgacaaggca ataaaggatg tagaggaaat ttccaagaaa tatgaattga
     1861 aagtggaccc ccgagctgtt gtccaggata tttccgtagg aatgcagcag agggttgaaa
     1921 tattgaagac cctttacagg ggagctgaaa tacttatact tgatgagccg acagcggtgc
     1981 tgacaccgca agagattgag gaattatgca ttattttgag gagcctggtg gaccagggca
     2041 agtcaataat actcatcacc cacaagctca aggaagtaat gtctatgagc gacagggtaa
     2101 caatcatcag aagaggcaag gtcatcgaca cgctgaatac aaaggataca aacattgatg
     2161 aacttgcaga aaagatggtg ggcagaaaag tgagtctcac ggtggagaag aaggaagctg
     2221 aagtttctgc tccgattctg gagatcaaag acctgagggt attgaacaac aggaagcttg
     2281 aggcagttaa agggatagac cttcaggttc atggcggtga aatacttgga atcgccggag
     2341 tggacggcaa cggtcagagt gaacttgtac aagccataac cgggctttca aaagcagaag
     2401 cgggagaaat tcttctggcc ggcaaggata ttacaaacct aactccgaag gaggtaattg
     2461 ccagcggagt ggggcatata ccggaggaca ggcataagag aggcctggta ctggcatata
     2521 gcctggcgga gaattccata ttggggagcc atcacatgaa gcagttcaga caaggttttg
     2581 ggctgaacta ccaaaaaata agggaatatt gcagaaggct tattaaggaa tacgacgtca
     2641 ggacaccgaa tgaacgggta ctggcaaaat ccctttcggg aggaaaccag caaaaactga
     2701 ttgttgcaag ggaaattgac agagatccaa agctttgtat agcttcacag cctacaaggg
     2761 gactggatgt gggggcaata gagtttgtgc acaaaaagct aatagagctg cgggacgaag
     2821 gcaaagctgt gcttctggtt tcccttgaac tggatgagat aatggattta tcagacagga
     2881 tagcagtaat gtatgacgga aaaatcgtag gaatccttaa tgcaaaggat gcaacagaga
     2941 agaagctcgg cattatgatg gctggcggaa gcgctcagga tgctgtatag ggggaatgtg
     3001 aaatggaatc tggaataaat gaatttaaag aaggactgtt gaatacgtta aaggaaattg
     3061 catatcccct tgtcgcaata cttatttctg ttattgcggg gggtattctg gtttactcct
     3121 ttggcagtga cccgataata gcatatactg ccatgataaa aggaagcctt ggggattgga
     3181 ataaaaccgg agagactttg gttaccgtta ccccgcttat cttgacaggc ctgtctattg
     3241 ctttcgcatt caaatgcaat cttttcaaca taggagtgga aggccagttt attatcggat
     3301 ctattactgc agtatgggca ggatgggctt ttgcgggatt gccggcaata ctgcatcttc
     3361 ctctgacact gcttgcaggc accgttgccg gcgggatttg ggcttcggtt atcggctggc
     3421 ttaaggccaa ggtgggctcc catgaagtaa taaatggaat aatgatgaat tacattgcaa
     3481 tgtatacgag taattttctg gtaaggacag tattgaatgt acccggcaaa gcctattcca
     3541 ttgaaattgc cgataccgct aagctttgga ggttttctca ggcttttgag cagtttaatc
     3601 attcaagact gcacattgga ataatccttt ccctgatagt agccctgatt gcttattata
     3661 tacttaataa gactgtaaga gggtatgaaa taagagctgt cggcttcaat ccgcatgcag
     3721 ccgaatacgg cgggataagc gtcaggaaaa atataatcct tgcaatgatt gtttcaggtt
     3781 tgtttgcagg gctggcagga agcataatgg ctacgggagt ccaatacagg gtaaataacc
     3841 tgttcggttt tacaggctat ggattggatg gaattgccgt ttcgctggta ggaaataacc
     3901 accccatcgg aatcatactc tcggcatttc ttttcggagt actgcaaaag ggcggccccc
     3961 ttatgcagat acaagggatt cctaaagagg ttgtcggtat cattcaggga ataatcatac
     4021 tttttgtagc tgcaaacttt gttaaggtag taatggagaa cattaaactg aacaaggact
     4081 tgaagagaac tgcagtgaaa gaggaggtgg aatagaatgg agggcgtagg cattttaacc
     4141 ataattggca tagtagttac agccagcata agatcggcta ctccgctgat attcgcctcc
     4201 ctgggaggca acttctccga aagatccggt gttttcaata taggcctcga aggaattatg
     4261 acagtcggtg cttttagtgc ggtatttttc tcatataaga caggttcccc ctgggcaggg
     4321 cttttaggtg ccatggttgt cggaggcttg ttcggactgc ttttggcgtt catgagcata
     4381 tatttcaaag caaatcaggt tatagtgggt actgcgataa atattctggc agccagcctc
     4441 actaccttcc tgctggttga aatctgggga aggccggggc agacggacaa tgttgaatac
     4501 tttaaagctg tggggccctt caatctgttt acttacttgg cgtttgtcat ggtaatcgta
     4561 tcatattacg taatgtataa gactcccttc ggattgagag tccgtgccgt aggtgaacac
     4621 ccaagagctg ccgatacatt gggggtcaat gtttatgtaa taaggtacat atgtgtaatg
     4681 ataagcggtg tacttgcagg aataggcgga gcatcacttt ccatagggtc aatgagcctt
     4741 ttcaaggagg gaatgatagc aggcaagggc ttcattgcac tggctgcatt gatattcggg
     4801 aaatggaatc cgataggggc ggcgttggca tgcctattct tcggctttgc tgttgcactg
     4861 gaaacagttg cgacaaattt gccggttccc agggagtttc tgtatgcgct tccatatata
     4921 atgacgatgt ttgcggtatc aggatttgta ggaagagcgg ttggaccggc tgcaggcgga
     4981 attccctatg aaaaaggtga aaaatgatat tatgataaat tatataatgc gcttcggcgc
     5041 attattcctt ttatttacag aagcaaataa ttatgacttg tccctcatat atgctataat
     5101 ataagtggat ttatgctata taatagtaat agataattta ttgcatagga tagtattatt
     5161 tcattgatgg ggggtgatga aatgagaatg ggatatgatt tattaataga acaacagcag
     5221 aagctgataa tgacgcctga actaaagctg gctctgaaga tacttcaatt acctgcagta
     5281 gagcttgagg aactgttaca gcaagaattg gaagataatc ccattcttga actggcggac
     5341 gataacaggg atgaaaaatc cgaagtacag caaaaacaag ataaaaagga aaaggaaaaa
     5401 gaaaaagaaa ttgattggaa ggaatacttc cagtttcaag gcaagagtta tgctgaggag
     5461 ggctttgaca gtgatgaggc tgctgaactc tcctatgaga atttcataac ctactcatat
     5521 acattgaaag atcaccttat ttcacagctg cgggtatccc aaataagcaa aaagcttaga
     5581 gacataggag agtatattat tgagagtctt gatgaaaacg gatatcttac cattacaaag
     5641 gaagatctgg cagccattct tgatgtgaag ccggaggagg tagaggaagc attaagtgtg
     5701 atccagacct ttgagccttt tggagtaggc gcatcaaatc tgaaggaatg ccttttaata
     5761 cagcttagaa ataaagatat ttatgataag aaaattgaag tgattataat taaccacctg
     5821 gatgatattg catccaacag gattaacaat atatcgaaaa agctctctat aagcattgaa
     5881 gaagctcaga aatacagcga tattattaag aaccttgaac caaaacccgg ccgggcattt
     5941 gaggagggta cctcaacaaa atatgtaaca cctgacgtat atatagaaaa ggtaggaaaa
     6001 gaatacgtag taataatcaa tgactattat ggttccaagc ttatgataaa tcaattttat
     6061 aaaaagatgc tgatttccga ggataagtca tctcaggcct ccgctttcat aagcaataag
     6121 ctgtcctctg caatgtggct gataaagagc cttgagcaca gaaaaaacac tttatataat
     6181 gttgtgaaag ctatattgaa ttatcagatg gatttttttg aaaagggacc catgtttctg
     6241 aaaacaatga cactgaagaa gattgctgaa atggtaaatg tacatgagtc aacagtaagc
     6301 agggcaataa acggcaaata tgtgcaaaca ccccggggga tatttgaaat aaagtacttt
     6361 ttcaaaagcg gtgttgataa tcaggacgga gaagcgattt cttctgaaag catcaaaaaa
     6421 atgattaaga gttacgtaag cggcgaggat gcaagcaagc ctctaagcga tcagcatata
     6481 gcagatttac ttgtgaagga aggatttatg atttccagga ggacagtggc gaagtacagg
     6541 gatgaactgg gcatcttgtc ttcttcaaaa agaaaaagat attaaaggcg gcaatgataa
     6601 ggccgctttt ttattttctt gcatacttta ctgataaatt ataaatttta acaaataata
     6661 caatattata ttgaaattgg aacatgcata taatataatt aactcaatcg ggacgttatc
     6721 attatatacg ggacaaaata tgaccatagg ggtaatgcaa tgaacgaatt tttgaattta
     6781 cagaaaagac tggttccgga aataatagag attacagaaa aaagatatag cattctaagg
     6841 gcaattgagt tcaatcagcc tgtagggaga cgcgctcttg cgggaatact cgggcagagc
     6901 gaacgttggg taaggagtga gcttgatatc ttgaagagcc agaatctgat ttatgtgcag
     6961 gctttgggca tggtggtaac agatgaaggc aatgaagtga taaacaagct cagggattac
     7021 atatcagaac ttaaggggct caaggacctt gaaaaggcag tcagggaaat gctcaacata
     7081 agagaattaa ttgcagtgcc gggggatacg gaaaatgagc tgcaggtagt tgttgaaatg
     7141 gccaaagcgg cttttgaata tatcaaagga ataatagagg ataatgatat aatcgcagtg
     7201 agcggaggtt attccgcatt gtcagttgcc gagaatgcac gcaggattga tgcacaaaac
     7261 attaccatag ttcctgccag aggcggaata aaaaccgata tggagcgcca ggcaaatatt
     7321 gttgcagaga agttagccaa gaatatcgga ggcaattatt atcttctgca tcttccggac
     7381 aatataaatg aagatatgct gaaaaggatg gctgatgatc cggatatagc caaggtgctc
     7441 aattacatcg ctgaaactga tgttttgata tacggcttaa gttcctttga atccatatcc
     7501 aggaaaagaa attttaccga agagcaggtt gaagagctta agactttgaa tgtcagggct
     7561 gaggcagtag gctgctattt tgattctaac ggaaatattg tatataaatc aggcagcata
     7621 ggtataaaac ctgagcattt agatagtata aaaactgtaa tgtcagttgg aggaggagcc
     7681 cgaaaagctg aggctatcgt attgacaagc aagggcagaa aaaacagagt agttgtaact
     7741 gacgaagctg ctgcccgtga aatatataag attttaaaag gaggaaatta aaatggcatt
     7801 aagagtagga atcaacggtt ttggaaggat agggaggccg gtttttaaaa tcgcttcgga
     7861 gaagtttgga gatgatgcag aaatagtttt aataaatgat ttaactgata cgaaaacact
     7921 ggcacactta ttgaaatatg acagcacata cggcaggtat gacagagatg tacaggctaa
     7981 agagcattca ttagtcatag acgggaaaga aattgagata tgtgctgaga caaaaccgga
     8041 aaacatcagg tggggtgact acggagttga cgtggttatc gaatccacag gcaggttcac
     8101 aagcaaggaa gcggcaatgg ctcatgtggc tgggggagca aagaaggtaa taatatctgc
     8161 tcctgcaaag aatgaagaca ttacgatagt aatgggtgta aatgaagaca agtatgatcc
     8221 tgaaaaacat gtggtaatat caaatgcctc ctgtacaacc aactgtttgg ctcctgtagc
     8281 caaggtgctt cacgaaaact tcggaattgt aaagggtttg atgaatacaa tacattcgta
     8341 tacaaatgat cagagaatac ttgatctgcc tcactcagac cttagaagat cccgggctgc
     8401 cgcattgtca ataattccga caactacggg agctgcaaaa gcagttgcat tggtgcttcc
     8461 ggagcttaaa ggcaagctta cggggctttc tatgagagtt cctactccta cagtgtcggt
     8521 agtagatttg gttgtcgacc tggaaaggaa tgtcactgtt gaggaagtga atgctgctct
     8581 caagagagcg gcggaaaatg agctgaaggg gatacttgag tattgtgatg aacctcttgt
     8641 atccatagat tttaagaagg atccgcattc ctcaatagtt gatgcactat ctactatggt
     8701 tattcaggat aacatggtaa agattatctc atggtatgat aatgagtggg gctattccaa
     8761 cagagtagtt gacttggtaa aatacatttc aaataagtag aatcgactgg gaggattctt
     8821 gttatgaata aaaagtccat acaggatata gatgttgacg gtaaaaaggt gctggtaaga
     8881 gtagacttta atgtgccctt agatgaaaac ggcactatca cggacgacag aagaataagg
     8941 gaagcacttc cgacaatcag gtatctgagg gagcataatg caaaagtcat attgatgtca
     9001 catctgggaa ggccaaaggg gaagttcaat atgaaatatt ctctgaagcc ggtggctgag
     9061 agattgtccg ggaaacttgg agccgaagta aggctcgccg gtgacgtaat aggtgaggaa
     9121 gtatcggcaa tggtaaacag cctcaaagaa ggggatgtaa tgctccttga aaacgtcagg
     9181 ttctatgaag aggaagaaaa gaacgacaaa gagttttccg gaaagcttgc agccttgggt
     9241 gatatatata taaatgatgc cttcggtact gcccacaggg cacatgccac aacagcaggc
     9301 attgcagaat acctgccctc cgcagtaggt tttctgataa agaaggagct ggaaataatg
     9361 gggaaggctc tgactgcacc ggaaaggcct tttgttgcag tactgggcgg agcaaaggtc
     9421 tcggataaga tcggcgtaat agaaaacctg atggataagg ttgatactct tttgattggc
     9481 ggcggtatgg cctatacatt cttcggggca atgggttatg agacaggcaa gtccattttt
     9541 gaagccgata aagcggaact ggcgggtgaa ctgctgaaaa aagcagaaac aaaaggcgtg
     9601 aaattgctgc ttcccgttga tactgtcgtt gcttcagaat tcagcaatga cgcacctaaa
     9661 aaaactgtac cgtctgctat gattccgaaa gatatgatgg gtatggatat cggggaggat
     9721 acaagaaaaa ttttctcgga aattgttaag aacgcaagga cagttctatg gaatggccct
     9781 atgggcgtat ttgaaatgcc gaattttgca aagggcacct ttgaattggc aaaagctatg
     9841 gctgaatgta ccgggacaac aatcatcggc ggcggtgact ctgccgcggc tgttgaacag
     9901 cttggctttg cggataagat gacgcatata tctacaggag gaggggcttc tcttgaattt
     9961 atcgagggta aggtgcttcc cgggatagcg gcaatagaga ataagtaatt tgggaggtaa
    10021 tattatgcgc agaccggtta tagctggcaa ctggaaaatg aacaaaacag taaacgaagc
    10081 aataaagctt gcggaggagc ttaaacctct tgttaaggga gctgccgctg aagtagcagt
    10141 gtgtcctgcc tttatttgcc tgaatgattt ggtaaaggtt ttaaagggaa gcaatataag
    10201 agttggtgcg caaaacatgt accataagga aagcggcgca tataccggtg agatatctcc
    10261 cgtgttctta aaggatatag aggttgaata tgtaatattg ggccattcag aacgcagaca
    10321 gtatttcagg gaagaggatt cggaaattaa caagaaggta attgctgcac tggaccatgg
    10381 attaaggcct attctctgcg tgggagaaac ccttgagcag agagaagcgg gggtgacctt
    10441 cgatgtcata gcagctcagg tgaaagggtg tcttgaaggc cttgagaaca gaaatacaga
    10501 tgacttgata atagcctatg agcctgtatg ggctatagga accggcaaaa ctgcgaccag
    10561 tcaggatgca aatgaagtca ttggattcat caggaagcag ctggccctat tgcttgggaa
    10621 tgatgcggct gacaaaacaa ggatactata cggaggcagc gttaagtcct caaacataaa
    10681 ggaacttatg gatatgcccg aaatagacgg ggctcttgta gggggagcaa gcttggacgc
    10741 ccaagagttt tcacggattg taaatttcaa tcagtaataa atatatagat ttaaggaggg
    10801 tactgactat gacagtaatt actgacatat acgcaagaga aataatagac tccagaggga
    10861 atccaaccgt agaggttgaa gttgagttgg agggaggagc tttaggcagg gctgcagtac
    10921 cttcaggagc atccaccggg gcttttgaag ctgtagagct tagagacggc gataagtcaa
    10981 gataccttgg gaagggtgtg ctgactgcag tgaataacgt aaatgaaata attgcaccgg
    11041 aaataatagg catgaatgca ttggatcagg ttgcaataga ccaggctctt attgagctgg
    11101 atgatactcc aaataagggg aagctgggtg ccaacgccat attaggagta tcactggctg
    11161 tggctcatgc ggctgcagag gcgctgggac ttccgcttta tcagtatgtc ggcggagtaa
    11221 atgccaagac tcttcccgtt ccaatgatga atatactaaa cggcggaaag catgcagaca
    11281 ataatgtgga tattcaggaa ttcatggtta tgcctgtcgg tgcggaaagc tttaaagaag
    11341 cattgagaat gtgtgccgag gtttttcata atctaaaatc cgtgcttaaa gccaagggct
    11401 ataacacggc tgtaggagac gaagggggtt ttgctccaaa ccttacttcc aacgaagaag
    11461 cactgcaggt tatcattgag gcgataaaga aggcaggcta tgagcccggc aaggatcttt
    11521 acatagcaat ggatgctgct gcaaccgagc tttacaagga ggacggcaag tatcatctgg
    11581 atggtgaagg agtagtaaag acttctgaag aaatggtcga ttactatgag aatcttgtca
    11641 gcaagtatcc gataatctcg ctggaagatg gactttccga agaagactgg gacggctgga
    11701 agcttcttac cgaaaggttg ggtaaaaaaa tacagctggt tggcgatgac ctgtttgtaa
    11761 caaatacaga gagactggaa aaaggcatag aaatgggagt agccaactcc attctcataa
    11821 aggtaaacca aatcggtaca ctgacagaga cccttgatgc aattcaaatg gcaaacaggg
    11881 caggttatac cgcggttgta tcccatagat caggcgaaac agaagacgta acaattgctg
    11941 accttgttgt ggctgtcaat gcaggacaaa taaaaaccgg tgctccctca aggactgaca
    12001 gggttgcaaa gtataaccag cttatgagga ttgaagaagg attggataca atgtctgaat
    12061 atttaggcgt aaatgcattt tttaatctat aggccatgac gtaagagcct gataaaaaat
    12121 tctggggagt cgtatttttg aattaaaagt gctatacaat ttaaaaatat gtgatataat
    12181 aatgggtgaa attatttcat cccattattg tattttatca gcctttgtgt taaaatggtg
    12241 tttgtagaat ttttcctgat aattatgtga ataataagcg gaaagagcga atgaatggca
    12301 ataaatgcac gttcttttgg cacaaacgga tgatctggat gaagatggga ggtgcaggaa
    12361 gtgagattgg ctatagtaat tttacagtac gttgtttgtt tggtattgat tgtaagtgtg
    12421 cttctgcagc cgggaaggag tgccgggctg tccggttcaa tagcgggagg tgcggaacag
    12481 cttttcggaa agaaagcaag caaaggctac gaagccttgt tttctaaact gacaacagca
    12541 agtgcaattc tgttcatggt tttatcggtt gtactggtac ttgtaaaata atatttcaat
    12601 aatgatagcc tgactttttg ttaggctata ttatcaatat ttcgtgtata cagtattaca
    12661 gtatatcaca tttatgtatt aggagggtta tttggatgga tttattgatt ctggctccgc
    12721 tgtcaggagt acttgctctg gtttttgcat ggtttttagc aaacagtatc gcaaagaaag
    12781 accccggaaa cgacaggatg aaagaaataa tgggctttat ccacgaaggg gcaatggcat
    12841 ttctattcag ggaatataag gtgcttgttg tatttattat tgtattggca ataataatcg
    12901 gagtattcct caaaagctgg ctgacagcaa tatgctttgt tgccggggca tgctgctcca
    12961 tacttgcagg ttattttgga atgcaggtgg caacaaaagc aaatgccaga acagcgaatg
    13021 ctgcacagag aggccagaat gaagctctcg ggatagcatt caggggcgga gctgtaatgg
    13081 gcatgtgtgt tgtaggttta ggccttctgg gtttagggac tttatatata tttataagag
    13141 atgcaagtat tgtgacaggt tttggcctgg gtgcaagttc aattgctttg tttgcccgtg
    13201 taggcggcgg gatatataca aaagctgccg atgttggtgc tgatcttgta ggaaaggtgg
    13261 aagccggcat acctgaggac gacccgagga atccggcagt tatagctgat aatgtcggcg
    13321 acaacgtagg tgacgtagcc ggtatgggag ctgacttgtt tgaatcatat gtgggttcga
    13381 ttatttccgc aataactctt ggtgcgatag tatatgccgg aaaggttgaa ggagtattat
    13441 tccccatgct tttggctgca ataggcatag tagcttcgat tataggaact tcttttgtaa
    13501 agaccgatga aaagagtaac ccgcagagcg ctctcagcag aggaacttat gggagttcga
    13561 taatcgtaat tgtggcatct tttatactaa gccgctatat gttcggtaat ctgaatgcct
    13621 tctgggcagt tgctgcaggg cttgtcgcag gatcgataat cggttttgta tcagaagtat
    13681 atacttcttc tgactataag tatgtaaaga aaattgctca acagtcggga acagggtctg
    13741 ctactacaat aataagcggt cttggagtgg gaatgctctc tacagtggca ccgataatct
    13801 tcatttgcgc tgcagtgctt gtggcatatg attctgccgg catctacggt attgctcttg
    13861 cggcagtagg tatgctttca acaacaggca tgacggttgc agttgacgcg tatgggccta
    13921 tagctgataa tgccggagga attgcggaga tggccggtct gcctgagtca gtgagagaaa
    13981 ttacagataa gcttgattca gttggaaata caacagctgc aataggaaag ggctttgcaa
    14041 taggctcagc ggcattgact gccctggcgt tgtttgtatc ctattccgaa gctgccggcc
    14101 ttaagacaat agatatcctt aaccctaaag taatggttgg aatgtttatc ggaggcgcgc
    14161 tgcctttcct ctttgccgct ctgactatgg aagcagtcgg caaggccgct aatcagatga
    14221 tagaagaagt cagaaggcag tttagggaaa ctgccggcat aatggaaggc acaaccaagc
    14281 ccgattacgc aaggtgtgtt gatatttcaa ctgctgcggc attaagagaa atgattgtac
    14341 cggggcttct tgctgtcata gttcctatag ctaccggctt tattctgggc aaaggtgctt
    14401 tggggggact tattgcaggt gcggttgtta caggagtact cctggcaatc atgatggcaa
    14461 attcaggggg agcatgggat aatgccaaga aatatataga atccggcaag atgggcggaa
    14521 aaggcagtgt tgcgcacaaa gctgcagttg tcggagatac tgtcggagat cccttcaagg
    14581 atacggcagg gccttcaatg aatatactta ttaagcttat gactatagtt gcattggtat
    14641 ttgcaccatt gttcatgtag tttcagcata tatccgaacc atatttgata atactaataa
    14701 tatactgtaa tattaaagag atggtagaaa ttatcatctc ttaatttttt attgtaaaga
    14761 atatattttc actgtggaat atacttatgc cggaggttat catgaaaaca ttacttattt
    14821 acctcataac cgtcaatatt gcagcctttg ctgctatggg agcggataaa tacagggcgc
    14881 agaggaataa atggaggata agcgagtcaa gcattttcgc tttaggactt attggagggg
    14941 gagcaggcat atatcttggg atgaaattat tccgccacaa gaccaggcat cttgaattta
    15001 cactgggaat acctgtaata ataattctaa atgcaattgc gttatggtat attttgcaaa
    15061 aacttgagta aatgagacac ggggagacaa ggggagacac ggggacgttt cgcgggacat
    15121 ggggacgttt cgcgtgtccc actggaagtg ggacagggga aacgtcccca tgtcccggag
    15181 aaagtccccg tgtctcccct tgtctcactg aatgttatga acaaaatatg ttcataataa
    15241 ttacatattg aatccgcgac cttgggggat gtttttcttg aacgatataa acagcaaata
    15301 tgcaaaagaa ttctatttta aaggcaataa cacaggggtg ctgcttatac atggatttac
    15361 gggaacaccc tatgaaatgc gattcttggg ggaattcctg agagataaag gctgcacagt
    15421 aagaggaatc ctgctgaaag gacatggtac aacactgaaa gatatgagaa gatgcagcta
    15481 tagggattgg atccatggag ccgtagaagg atacagatcg ctgaagcagg aatgtgatga
    15541 ggtttttgcg gttggatttt ccatgggagg attgctgtcc ttatacctgg caaggaacta
    15601 tgatataaga ggtgttgtaa ccctttcggc acctataagg gtatatagca gagcagctgc
    15661 tttggctttt atagaaagaa acctcagaaa tcacatgact ggaaagactg agaacaggga
    15721 aataaatgta atcggttatg agagagcgcc attaattagc gtgcatcatt tgttcaagct
    15781 gataagatat gtgaaagcaa acctgaagta tgtaaaaaaa ccggtgctta ttatgcaatc
    15841 atacggtgac agggcggtaa gcccgaaaag tgcaaatatt atatacggca gcataggttc
    15901 gaaggataag agtataatat atttacacaa aagcgggcat gtaataacct gtgacaagga
    15961 aaaagagcag gtttttaagg aagtatacag ttttataaag aataaatcca cttttatcag
    16021 agaagcggat agaaaagaag agaggtgcag cagtgtttaa tacagagaaa gaaataaacc
    16081 aggaaataaa gagcaaattg ttagctttca tgagagagga cgcatacaaa cccttgtctc
    16141 atgatgagct tattcaggca tttcaggatg attcacttca ggtgccggaa ttggacgaag
    16201 tgctgaattc ccttctggaa gaagggagca taataataac aagaaaaaac aaatatggcc
    16261 tgacagaaaa gatgaatatg gttgtgggac gcatacaagg gaataaaaag ggttttgggt
    16321 tccttattcc ggacaataag gatcttcgtg atttattcat acctgcagaa aatctcaatg
    16381 gagcaatgaa cgatgacaga gtaattgtaa ggcttacggc aggtgagtcg gggactggga
    16441 agagagaggg agaggtagta agaatactga aaagagcgaa tgccaaaatc gtaggtacct
    16501 tcgaaaacgg caatggctat ggatttgtgg tgcctgatga tcaaagaata tatcaggatg
    16561 tttttgtatc caaatctgac ttcaatggtg cgaaagacgg atataaggtg gttgtcgaga
    16621 taaccaggtg gccggaggca agaagaaatc cggaggggat tgttgttgag gtattggggc
    16681 ataaggatga tgtaggcacc gatatcatat ctattatacg gggccacaat ctgccggagg
    16741 agtttccaat cgaggcagag aaccaggccg gaaaaatacc tgaactggtt agtgatgcgg
    16801 atatagcaaa acggagagac ctgagagata aaacaatagt tacaatcgat ggggaagacg
    16861 caaaggatct ggatgatgcc gtatcactgg agatacttcc tgacggaaat tatatgctgg
    16921 gagtacatat agcggatgta agccagtatg tatttgaaaa ttcacatttg gacaaggaag
    16981 cactaaaaag aggtacaagc gtgtatctga ttgacagggt ggtgccgatg cttcccaaaa
    17041 ggctttcaaa tggtatttgc agtttgaatc ctcagataga aaggctcact ttgagctgcg
    17101 atatggttat agacaagaag ggcaaggtaa aagaatatga gatttatgaa agtgttataa
    17161 agacaagaga aaggatgacc tatgaaaatg taaataagat acttgccggc agtgatccca
    17221 tgcttacaga aaggtatgat tatcttgcag ataccttcag gcagatggaa gagcttatgc
    17281 tgatactgaa taaaaaaaga cgtttaagag gaagcgtaga ctttgaattt gatgaaacca
    17341 aaatcatatt ggatgaaaag ggcaggccta tagagataaa gccctacgaa agaggaatat
    17401 ccgaaaggat aatcgaggaa tttatgctgg tatgcaatga gacagttgca gagcatatgt
    17461 attggaaaga gacacccttt atttacaggg tccacgagga tccggatgca gaaaagcttc
    17521 aagcctttaa tgaatttata tttaactttg gttatcatct gaaaggaata gctgagatac
    17581 atccaaaagc gctgcagcag cttaccgacc agataaaggg aaccaaagag gagcggataa
    17641 taaatacact gatgctgcgt tcgctgaaaa aggcaagata tacttcggaa agcatgggac
    17701 atttcggcct cgctgcaaag tattactgcc acttcacttc tccgataaga agatatcctg
    17761 acctgatgat ccacaggatc attaaggagg atattcacgg aaaactcact gaaaagaggc
    17821 tgaagcatct tcactcaata attgagagta tcgctgagca atcctccatc agggagcggg
    17881 cggcagatga agctgaaaga gaagtagaag acctcaaaaa ggcagaatat atgaaagaca
    17941 ggatcggaga ggaatatgac ggaataattt ccggcgtcac ttcctttgga atgtttgttg
    18001 aactggaaaa cactatagaa ggcttggtgc atatgagcaa tatggaggat gattactatc
    18061 agtttgatga gatgcatcac atgcttatcg gtgaaaggcg gagaaggacc ttcagaattg
    18121 gcgatagtgt aaggataaaa gtcctgaatg ctgatattgc taatagaaca atcgattttt
    18181 cattggttta aactgattta acattttttt tcattgatat tacagttgta tttcgttata
    18241 atatataagt tgactccggt actaatgatg ttataatatt agtgcatgct catatttaaa
    18301 atgaaagcat ataacatttt gaaaaaagcg taatactttg taaagttggg gtaacatggc
    18361 acaagtcaac attaagacaa ttgcacaaaa taaaaaagcc agacatgaat acttcattga
    18421 ggatacctat gaagccggta ttgagctttc aggcactgaa gtgaagtctg taaggaaagg
    18481 cagcgtaaat ttgaaggaca gctatgccgc aatagataaa gggcaagttt atgtttacaa
    18541 tatgcacata agcccgtatg agcagggaaa tattttcaac aaggatccta tgaggacaag
    18601 aaaactgctt ttgcacaaat atgaaattaa taagctcata ggatatgttc agcaaaaagg
    18661 ttatactctt ataccgcttt cgatttatct taaaggcagc ctggttaagg tgcagcttgc
    18721 aatagcaaaa ggcaaggagc tccatgacaa gcgacatgac atcgcagaac gggatgcaaa
    18781 aagagaaatg gaaagggaat tcagaggcaa aaattattag acacgggggc gtaatggttt
    18841 cgacgggggt agttgaggcc gcggcagcga gcggtggtcc cagaggcacc taaaaaagct
    18901 ggaaacttaa atttaaacgc agaagataat tttgcattag cagcctaagg gctgctcgtt
    18961 caacccgggg ttcccgcacc ttgggattgg gcgtcattta gcggggtacg tcactgccaa
    19021 agctttgagg cagctgtcgc attatgaagc tactgaagct gtaagcctgc ttttgggcgt
    19081 gcagcggagg gaatgttaaa gcaaaagctg cgctcggaga agctgtgacc aagatgcttt
    19141 cggacaggag ttcgattctc ctcgcctcca ccatattttg tccgaaaacc cttgattttc
    19201 aagggtttaa tttgtttttt gaagagaaaa acaccaacat atttaaagaa aaaatcaggg
    19261 agcctactgc atagctgcaa ttaattccat aattcccacc atatttattg ctctttacag
    19321 attctaggca agaaaactaa gccccagttc tgcatgatca aggttatcaa caaatgcatc
    19381 aataaccctt accgggttat cctctccaac atagtcttca atgcaatcgg gaaacagtgt
    19441 aatctgtgat ctgtcctggc cttgaatata cttcattttc agcaccaccc taaataatct
    19501 tgttatatct attataccat atttcatagt gcctcaggcg ttgctgtgac tgacttttcg
    19561 gacagtctcg cccaaaaaat atgtatttac ataataacgc tgtattcgat gaatacagcg
    19621 tttgtgttta tgtgatgaaa caagggtaca ttatgtttgg acacatgtcc agcgacgtta
    19681 ttctactctt tggacattaa atcagcaaat atctcagcaa ttttcggatc aaattgtgta
    19741 cccgcatttt ccttgatgat ctcaatagct gccgctggtt ccattgcact tctgtatggt
    19801 ctgtcatttg tcatcgcgtc ataagcatca acaacagcaa tgattcgcga aagcagagga
    19861 atgctttcgc cgcataagcc tttgggataa ccgctgccat tccaccattc ctgatgacag
    19921 agaatccctt ctgcaatagg cacaagttcc ggagaagtca tggcaatccg gtatccgatt
    19981 tcgggatgcc ttttaatctc agcccattct gattcgctaa gtttaccggg ttttgtcagg
    20041 atattatcct tgattcccac ttttccgata tcatgcagtg tcgcaagcag ttcgagtttg
    20101 tccagctcta cttgtgataa attcagtgcc atacctatcg ccttggatag ttctactaac
    20161 ctttcggcat gttcttctgt ttcatggctt ttttcaaaca atgcagcctt tattgaagca
    20221 acaatagtac tgtgtacact gttagcctca agcagcttgc gctggtacat atgatcttcc
    20281 gcagttttta atatgtacat gatgtcctct tcaatctttt cttttgttgc ataacccagt
    20341 gatacactat gctgatacat ttcgaaattg ttttcagaat caaattttat cagtgcggtt
    20401 tgaatgttct gcaatacttc aagtgctgta acactgtcag ttctaggcat gattattccg
    20461 aactcatctc ctccggttcg ggctaatatg tcaccttgcc ggcagcagcc ttgtattatc
    20521 atcgcagatt ctattattat tttgtctcct tctgcgtgac caaaagcatc atttattaac
    20581 ttcaagccat ttatatctcc ggttattaca gacagaggca attggccttc tatatccaaa
    20641 cgtttcagtt cagtttcgta gtaccttcga ttatataagc cggtaaggta atcatgcctg
    20701 ctttgaaatg taagctcttc ttccgcttgt tttttagaag taatatcaat tgaaaatcca
    20761 agaaaccgat tctccgatat ctttaccgca tcgaccgtcc accatcttat cgagccgtct
    20821 ttatgtaaat attttaattc tgtactgagt ttgcccgtat caagcattga agtgaaacag
    20881 cgcatcgcct cgttcataga ctcttcggct gtaatatctc ttatagtcat ttcaagcagc
    20941 tgctctcgac tatatcctgt tataattgag gcggcgttgt ttacttctac atactgtcct
    21001 tttttatcga caacaaaaac tgcatagggt gcattttcga tgtaactgct atatttcttt
    21061 tcgctttctt ttaaggcatg ctctgcgcgt acttgggttg tgtcatctga gaaggtacat
    21121 gcaaattgca ttggagccgg ctgataggcc ataacacgga agtatttgtc ggttgcggcc
    21181 gcataatttt caaaactaac tgattcaccg gtcagcgcaa cctttccgta ggtttcaatc
    21241 cagtatgatt ccgtattcgg aaggatttca agtacattct ttcccagaat atctgctgct
    21301 ttcagcccag tcatcttttc aaaggccggg tttacagcaa gaaacctata atcaattggg
    21361 gttccttggt cgtcacaaat aatttcgtgc aaagcaaatg catcaaccat ttcattgaac
    21421 aaaagctgat aattctgatt ggcttttagc tcggcagtga tatcttgaaa agtagtatat
    21481 accttggaag gggcagcttc tccatcttcg aataatggga tggcatttac ggaaatccag
    21541 acataatcct tgagcatagg ctgatataca cccagtataa ccgggccata tggcttacct
    21601 gtccgtagag caatcataga aggatgatcc attcccgaca attccgaacc gtcttcgcga
    21661 attgtcctcc atctcggatc aagagacgac attcccaaca tttggtctaa cggaatacct
    21721 aatatttttt cagctgcggg gtttgccgat attatccttc catcagccgc ctgataaata
    21781 acaccttggg ccattgtctc atatagtcgt cggtatttct tttcgcttga ctgcaggtct
    21841 gtctccacct tcttctgcct ggttatgtct ttgagaatac agaaagtttc attaaaatct
    21901 ccattaggct cataaactat attaccatca actgcaataa atagagattt cccgctctta
    21961 tgcagcattt caaactcgct gtgaatttgc tttgtttctt ctaattgtga aaacttctca
    22021 agaaattctt ccttatacgc cgggtgaata aaatctccaa accactttcc tataacttca
    22081 tcccttgaaa acccggaagt attaagccac tgttgattta cgtcaattat attcccgtca
    22141 atgtcaagag attcatatcc aagcggggct ttatcaaaca acagtcgaaa acgttcttct
    22201 gactgcttaa gttggtcccg cacctgcaaa aatacctgct gttgggttag cagaagataa
    22261 atcggtactg ttgcagccgg gtatattaaa agtactggca aagtgatttc acggattaca
    22321 tttacactgg ccggataggg taaaaggttc atacaagcta tcatggcagc atggaccaaa
    22381 aggctcatag cataaacatt taaccagcgc agcttcgttg ccgcagaatg tatccaaagc
    22441 cgccacacta agccaataat agcactggat ataataacag caacacccgg caatgcacca
    22501 gcaccacctt gaaggattct taagatgata gcagcagcta ttgttataat cgtaggaatc
    22561 ggtccaaata ctaatgccgt tacgctaatc agaatagacc gtgtatcaaa ttgtactccc
    22621 aggtaaaact taaatggcac tgacataata gcgatacata acaaagcaat taatatcccg
    22681 cttactattt gcttaaatcg agtgtttctt gtatttataa gactggttag ctgatagatt
    22741 acgggcaaaa gcaataatag agcggcgttg ttcagtaatg caatcattgt tggctgcata
    22801 tgacacctcc tttgttatag atcggcttgg cttatttggc caggtatttt ctctttttca
    22861 acgaaagatg aagccgaagc acaccctata ggctattatt gttgcttcaa cacatagatg
    22921 cctttatagt taactgcact tccgaaactt tcaaattatc tgaattttca acattttatt
    22981 atgtattata taataatata aaaaaattgt cactatctat aattaaatta ggctggtgaa
    23041 ttaacacact agttcaaaat cactttattc gatatcagca agtaggcgga tttcctgggc
    23101 tggctttatc tcaaaatgat ttaatggcac ataaagtaat gcataaggac gtcgtggatg
    23161 aagtattaaa aagagatctg ccattgcttt ataacatcag aagggtaaaa tgctggagta
    23221 taatgacccc acttgtcaat acagaatttt ttgcagtctt agttaggt
//
LOCUS       DAOOAB010000041        21630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3199_length_21630_cov_15.949988, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000041 DAOOAB010000000
VERSION     DAOOAB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21630
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_21630_cov_15.949988"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PKU88_09150"
     CDS             complement(<1..1599)
                     /locus_tag="PKU88_09150"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPX69479.1"
                     /translation="MSKRIYIFDTTLRDGEQSPGISLNSNEKLKIARQLSELGVDAIE
                     AGFPAASKGDYEGVKSIAKNVRSSIIAALARAVRQDINTAWEAIEYAERPRIHTFIAT
                     SDIHMKYKLKMKPEDVLEKTAEMVAYARNLCPEVEFSPEDAARTDKEFLYRVIEEAIN
                     AGARVVNIPDTVGYTTPVEFGGLIRSIRNNVSNIDKAIISVHCHNDLGMAVANSLAAV
                     ENGADQVECTINGLGERAGNAALEEIVMALKTRKDFYNVYTGIVTEKLYRTSSMVSFA
                     TGVHVQPNKAIVGVNAFAHESGIHQHGIMSNSETYEIMTPESIGLKQSKMVLGKHSGR
                     HAFEEKLKEMGYSSLSQEEINKAFIQFKDLADKKKCIMDRDIEALITQESRKAYEKYR
                     MEYFQVSSGSDITSTSTVRIRCNDDVYEEAACGDGPVDATFNAIERATGVRVKLDEYV
                     IKASTEGKDALGEVTVKISKDKTRSVGYGVSTDIVEASALAYINAINNMESRVEEPEE
                     SEVKGFEFVKSRNYFGAGGDERWEL"
     gene            complement(1622..2623)
                     /gene="ilvC"
                     /locus_tag="PKU88_09155"
     CDS             complement(1622..2623)
                     /gene="ilvC"
                     /locus_tag="PKU88_09155"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPX69480.1"
                     /translation="MSNVKMYYEKDANLEVLKGKKVSVIGFGSQGHAHALNLRDSGVD
                     VCVGLYKGSKSWESAESHGIEVYEVAEAVKKGDFIIMVTPDEKSAEIYKESVEPNLRE
                     GKSLMFAHGFNIHYGQIVPPENIDVFMVAPKSPGHLLRSQYQEGKGVPCLIAVHQNYS
                     GNAKVNALAYAKGIGGTRAGVLETTFKDETETDLFGEQAVLCGGVTELMKAGFETLVE
                     AGYAPENAYFECLHEMKLIVDMIIQGGFGYMRHSISDTAEYGDYVSGKRIITEDTRKE
                     MRKILSEVQDGTFAKNWLLENQVNRPSFNAMRKKEQSHQLEQVGKELRKMMSWINKK"
     misc_binding    complement(2678..2877)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3311..4243
                     /locus_tag="PKU88_09160"
     CDS             3311..4243
                     /locus_tag="PKU88_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HPX69481.1"
                     /translation="MNNFTDYLDINREVLDGLRYYKPVVALESTIISHGMPYPDNIET
                     ALSLEKIIRENGAIPATMGIIKGRIKIGLDREEMEFLAKAKDIIKTSRRDIPFVVGMG
                     LSGGTTVAATMILSQYAGIRVFATGGIGGVHRNAEKSFDISADLTELSNTNVAVICAG
                     IKAILDIGLTLERLETFGVPVIGYRTDDFPAFYTRSSGFKIPMRAGSPRECAKIMKTK
                     WDLNLDGGIVVANPIPKQYEAQKDNIDKAIELALFEADKNGITGKAVTPYLLGKVKEI
                     TAGESLAANIALVRNNALLAAQIAVEYSKLENGS"
     gene            complement(4486..5538)
                     /gene="mtnA"
                     /locus_tag="PKU88_09165"
     CDS             complement(4486..5538)
                     /gene="mtnA"
                     /locus_tag="PKU88_09165"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPX69482.1"
                     /translation="MKKIFEAMRSVKWENNELIILDQTKLPDTTEFIKLDKAEDVWDA
                     ILHLKVRGAPAIGIAAAYGLCVAMRDTGAKSHEEFMTELKKNSQYLASSRPTAVNLFW
                     ALRRMERAAFDNKNKTVEELKEILIREANAIRDEDESMCRAIGENALTLFHDGMTVLT
                     HCNAGGLATAMYGTALAPIYLAKEKGWDIKVFADETRPLLQGARLTAWELMQAGVDVT
                     LICDNMAAAVMAKGLIDAVIVGCDRVAANGDTANKIGTYGLAVLAKAHNIPFYTAGPT
                     STIDIETPSGNEIPIEERKPDEITCGLGRRTAPLGVKTYNPAFDVTPHELITAIITEK
                     GVVRQPYDEGIRKLFE"
     gene            complement(5535..6386)
                     /locus_tag="PKU88_09170"
     CDS             complement(5535..6386)
                     /locus_tag="PKU88_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPX69483.1"
                     /translation="MAVLDCREAARSIKEELAKKVQLLAAEGLKPKLGIIRVGARPED
                     ISYEKGIIKNCESIGISCEVTALSDDISNEDFMKALEAANSDRSINGILVFRPLPAHI
                     DEDRARNMINPAKDIDCMNPYNLAKVFEGDDSGFEPCTPAAVMEIIRFFGINLQGENV
                     AVIGRSMVVGKPLSMMLLKENATVTICHSRTKDISSIVSRADIVVAALGKARAVGADY
                     IKPGAVVIDVGVNGAPEGGICGDVDFEKVKDIASKITPVPGGVGAVTTLMLLKHVVEA
                     AQQGSAL"
     gene            complement(6417..8615)
                     /locus_tag="PKU88_09175"
     CDS             complement(6417..8615)
                     /locus_tag="PKU88_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69484.1"
                     /translation="MNKSKNRFRKLIFLIVIVLFIGGSLIPAYCREEPGKMPSFSGGA
                     GTPEDPYLISTAEDLLAFSEVGWNAGYMEASYKLTGNIDMGENTWNPVGVNGLSFSGT
                     FDGNGKTITLRNIADSRNMGLFGGTDRRSSIKNLTVSGQISKAISSEEEACFGLIAAW
                     AEGSIENCITEGSVDLSVNVPGNFYFGGAAGHLLGRIDSMINKADLTIKISGGDRLYM
                     GGITGDAMGKEAGIFNLTNQGNITATFDGIGAVGGLAGFCGFGAEAENLLNQGNVTVM
                     QTRAPNSSRSAAGGIVGELRDSGMDKALNKGSIYFEHSGPYANEEIMAGGIAGESEIA
                     ELKNVGNEGNVETKIARIQLAIGITKPGREVTIENAYSRGRIYASTTDSKGELYVMGI
                     GEEVPTNNFYFSGTVRLKAGKKNEEDGDAFANIRLGAKTGIYNYCYWDSKFSPFPAYP
                     FFNKAAVTSKAVNTATGKLSSPVSIGGRQYDNISAALNAWVDMQKGGYLKWTKGAEPS
                     FEWTFGYQIPDFMQYKNGREGKWLNTSDWACEWMDKADRLSIIPDILLNQDMARGITR
                     KEFSALAVKLYENLKGSKIAVDLASPFTDTSDSDVIKAYSLGIVSGIGNGLFAPDASL
                     TREQACTMLTRVYKAVYWEGWALGEDAAYDRHVLDTAGVAKFSDDELISGYAKDSVYF
                     MVKNNIITGIGNNMFGPAPKEGQEGSYGRASREQAFKIAVAMIECFNEFR"
     gene            8789..9883
                     /locus_tag="PKU88_09180"
     CDS             8789..9883
                     /locus_tag="PKU88_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPX69485.1"
                     /translation="MISEFIEMAKVSSLSLKEGRFASLLAEKLRGLDFEVYIDKAGEL
                     AGGDTGNVLGRLKGTLDTQPILLCAHMDTVVPGENINPIIRDGIVYSDGTSILGGDDK
                     AGIAAILEAVRYIKENNIPHGDIEVAFTICEENGMFGAKHLDYSWLRSEFAFVLDSSG
                     AVGSVIVQAPAQIKTLSVIKGKAAHAGLAPEKGISAIQAAARAVNNMKLLRIDEETTA
                     NIGRINGGGATNIVCDKVEMEMEARSLTRSKVEAQAKHMLECIDDACREFGAEHETTQ
                     QLSYTELNIPKDAEVIKLVDSAIKRIGLETRLESTGGGSDTNIMSGKGIQAVTLGIGM
                     TNAHTTSEYIAISDMEKTAELVASIIQEAR"
     gene            complement(9880..10269)
                     /gene="mutT"
                     /locus_tag="PKU88_09185"
     CDS             complement(9880..10269)
                     /gene="mutT"
                     /locus_tag="PKU88_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HPX69486.1"
                     /translation="MYIVTAAAIIRDGKVLIAQRQQGSHMEYKWEFPGGKLEPDETPE
                     ECIIREIKEEMDIDIEVDDIYKVVKFRYEEKDILLLCYLCRIIKGDLKAIECNDFRWV
                     TKDELPGFDFVPADLPIVEKLINDKRL"
     gene            complement(10271..11824)
                     /gene="pckA"
                     /locus_tag="PKU88_09190"
     CDS             complement(10271..11824)
                     /gene="pckA"
                     /locus_tag="PKU88_09190"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPX69487.1"
                     /translation="MNLDNLGAKVHRNLTVGKLVEIALKKEGAALTDTGALSINTGKY
                     TGRSPGDRFIVDDPGIHDKVDWGKVNVPISGEKFENLYNKINEYIKDKELFVFDGFVG
                     ADKNYRMPIRIINEYASQNLFAQNLFIKPKEGELEGFEPEYTVIAAPGCHADAEADGV
                     NSEAFIILNFAKKIVIIGGSMYCGEIKKSIFTIMNFILPMKGVMPMHCSANIGEDGDV
                     ALFFGLSGTGKTTLSADKGRRLIGDDEHGWTEDGVFNFEGGCYAKCIRLSKENEPQIW
                     NAIRFGAVTENLVLDPETGVPDYDSEKFTENTRAGYPVTYIDNAVEEGVGGNPSTVIF
                     LTADATGVLPPIARLTKEQAMYHFMSGYTSKLAGTERGIIEPTATFSTCFGAPFMPMP
                     PQVYAELLGKKIDKNNARVFLVNTGWSGGSYGVGKRMKLSYTRAMVNAAISGELDNVG
                     YDTDPIFGLAVPAECPGVPAEVLKPVNVWQDKAEYEKTALKLAADFNANFRKFRGVSE
                     DIVNAGPKV"
     gene            complement(11978..13501)
                     /locus_tag="PKU88_09195"
     CDS             complement(11978..13501)
                     /locus_tag="PKU88_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HPX69488.1"
                     /translation="MAKRELKSKSLMIQGTASSVGKSLMCTAICRILARDGYNVNPFK
                     SQNMSLNSYITIEGHEMGRAQVMQAEACGKLPEACMNPILLKPVSDRKSQVIIEGGVY
                     ADMDAVEYFNFKPRLKERISDIYRRLEDRSDIVVIEGAGSPAEINLNHEDFVNMGMAK
                     IAKAPVLLVGDIDRGGVFASLAGTMLLLSPEEKKLVKGVIINKFRGSQEILKPGIKML
                     EEIINVPVLGVIPYFSMNLEDEDSVSEWLKSGPEKGGELDIAVIRLPYMSNHTDFNAL
                     RLHEDVSLRFAEIDKELGEPDVIILPGTKSTIHDMLALKNTGMDKKILKCHSRGSVVF
                     GICGGYQMLGKEISDKFKAESDNERSEGLGLFNAVTEFRKNKFTTLSKGRDNIFGAEV
                     RGYEIHMGETAGTDAENSFITISERGGKGVFGRDGMINKDLTVFGTYIHGIFDSSEFT
                     RSFLNLIRRRKGLVPLENLAPDYWVYKEGQYDKLAETVRSNLDMDKFYEILKTGIED"
     gene            complement(13831..14442)
                     /gene="cobC"
                     /locus_tag="PKU88_09200"
     CDS             complement(13831..14442)
                     /gene="cobC"
                     /locus_tag="PKU88_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HPX69489.1"
                     /translation="MKGSKTEIYLIRHGETDMNIKGVYYGWTDVGLSEKGIMQAEDLA
                     DILHDVQFDAVISSSLIRAVETASIVSGYTPDMIIKDDRLRELNFGDWEGKHHRELKV
                     RYKEALEKWGSDWRKSAPPGGESFFEIYIRVKSSIEDILKEYRGKKVLIVSHQGTMRI
                     IPLVLLGLTEDAYWSFTAEQGRYSLYEINEEGRCIIRRINSGR"
     gene            complement(14439..15191)
                     /gene="cobS"
                     /locus_tag="PKU88_09205"
     CDS             complement(14439..15191)
                     /gene="cobS"
                     /locus_tag="PKU88_09205"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019279106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPX69490.1"
                     /translation="MRIFLITLQFLTRISVNKNLKATEEDFAKGVAFFPIVGLIIGAF
                     NLTILIIASNILGGVFPVICCLIAGTAITGGLHADGLADTCDGIFSSRDRDRMLEIMR
                     DSRIGTNGTIAIIFDYLLRMSLLAMLTGKALYGAVLLSPVAARTLLVLLMKVSVYARS
                     GSGMGGLYLEKQTMRSTVLAAALGIIIILGFGHIWGLVVFGICTVICFLYRSFIYSKL
                     QGMTGDTLGAANEILEVSFILITAVMAGRGII"
     gene            complement(15207..15767)
                     /gene="cobU"
                     /locus_tag="PKU88_09210"
     CDS             complement(15207..15767)
                     /gene="cobU"
                     /locus_tag="PKU88_09210"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPX69491.1"
                     /translation="MGKSILITGGARSGKSSYAEKLAKELGGNVLYIATSIPFDEEMK
                     LRVKRHKESRPREWDTFEGCKGLGRVISEKGSQYKGILLDCITVMVTNLLLEYTGMEG
                     NDCSNEDFEKAEKEIEREIAELMAGIGNSEASVIIVTNELGSGIVPENPLARAFRDIA
                     GRTNQYIAERCDEVYITVCGIPLKLK"
     gene            complement(15932..17002)
                     /gene="cobT"
                     /locus_tag="PKU88_09215"
     CDS             complement(15932..17002)
                     /gene="cobT"
                     /locus_tag="PKU88_09215"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPX69492.1"
                     /translation="MEIFERTAAGIRPSYEESKKEAAERLDKLVKPIGSLGKLEDIAV
                     KISGITGKQKNSFEKKVTIIMASDNGVAEEGVSAAPQAVTALMTINFLKGITGVCVLS
                     KHAGADIRVVDIGVGSDLEYPGLINRKVRKGTSNMAKGPAMSKEEAISAIETGIEMVS
                     QLVKEGYNLFGTGEMGIGNTSTASAVAMAFLGCSAEEAVGKGVGLTEEGYASKKSVIE
                     RAISINKPDPYDPIDVLSKVGGFDIAGMTGCFLGAAYCRVPIVIDGFISAAAALAAYR
                     INPLVRDYIIPSHLSAEPGYSLVINEIGLEPLLDLNMRLGEGSGCPLAFNIIEAAEAI
                     ISDMATFEEATIKDDFLIDIRK"
     regulatory      complement(17107..17281)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            17576..17875
                     /locus_tag="PKU88_09220"
     CDS             17576..17875
                     /locus_tag="PKU88_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69493.1"
                     /translation="MGKEHRRDKEFRIKHFPDNIRDLLCLLADNEDNRATIILNTCDD
                     CVQENVEVVAVIGNLAVFRCHDKFKFVDIHCICEVIVDCETILSELLESKSECKC"
     gene            complement(17923..18375)
                     /locus_tag="PKU88_09225"
     CDS             complement(17923..18375)
                     /locus_tag="PKU88_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPX69494.1"
                     /translation="MLQFIYQVVKKVTNSRKIEDLHPVVQEICRKHIEACKRQGVQII
                     VTNTLRDSEYQEYLYSQGRTRPGNIVTNMRLIGPHGFGLAYDVVPIVKGKAVWNNNRY
                     WRIIGIEGKKLGLTWGGDWKSIVDKPHFEYTGGLKAADLRAGKRPSWW"
     gene            complement(18458..19867)
                     /locus_tag="PKU88_09230"
     CDS             complement(18458..19867)
                     /locus_tag="PKU88_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPX69495.1"
                     /translation="MKEKNYVSPILDRIRKQTFEEDMVLAHDLALRYVKQVNDMRVFP
                     DKKAVGNLSVFEEALSEEPEDTKNILNMLGKYGSPATVAQTGGRYFGFVCGGILPSAL
                     CSKWLSDAWDQNSALFVLSPIASKLEEVCESWLKQLLKLPDDTAAGFVSGSSTATICG
                     LLAGRNYLLGNLGYDVTKEGLFNAPQIKVVVGEEAHSTVFKALSIIGLGNAKLIRVPT
                     DGQGRIKADEIPEPDNRTLLILQAGNVNTGAFDDFDSVCRKANAAGAWVHIDGAFGLW
                     AAACEKMNRLTKGMELADSWSVDAHKTLNAPYDNGIVLCRHKDALVNAMHMTGSYIIY
                     NENRDGMLYTPEMSRRARAIDLWATLKGLGRKGAAELVEELHCKAVYFSRLLEEGGLQ
                     ILNDVVYNQVLVYYDNDRKTEALIKGVQESGVCWLGGSKWSGKSVMRISVCSYKTTYD
                     DIEKSAEDILRVAALVSDK"
     gene            complement(19925..20341)
                     /locus_tag="PKU88_09235"
     CDS             complement(19925..20341)
                     /locus_tag="PKU88_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPX69496.1"
                     /translation="MIKRHKSVCYCINLRRAANAVTDLYNRVLLPIGLTVSQYSLLIN
                     IGRLKACSVSDLAGYVGLERTTLVRSLKPLFKLKYIEDVSEKGRRSRLLKVTGAGQQV
                     LNQGEVLWRTAQAELEQKVGKEKLEQLSEILAQLVE"
     gene            complement(20553..21185)
                     /locus_tag="PKU88_09240"
     CDS             complement(20553..21185)
                     /locus_tag="PKU88_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPX69497.1"
                     /translation="MSAESTIKGYLIVHLEHILDEIGEDETIKILLGFSCPLNSDVEK
                     FLHHKAIEFAKQSLSQTFLVFTSYKDEPTLVGYFTLANKTILVDKAVLRGNVKRKIGR
                     FGTYNPVIKKYVFSAPLIAQIGKNYKYNEQNLISGDELLKMACDKIAEAQFVLGGKVV
                     YLECEDNEKLKNFYSSNGFVEFGKRKLDRDEKDDLSGEYLVQMLKYTKKC"
BASE COUNT         5550 a         5577 c         4026 g         6477 t
ORIGIN      
        1 caattcccaa cgctcatcac ctccggcccc aaaataattc cggctcttca caaattcaaa
       61 accctttact tcgctctctt ccggctcttc aacccttgat tccatgttgt ttattgcatt
      121 tatatatgcc aatgcgctgg cttcaactat atccgtgctg actccataac ctaccgacct
      181 tgttttatcc tttgaaatct ttacggtaac ttcgccaagg gcatctttac cttccgtaga
      241 agcctttatc acgtattcat caagctttac ccttacacct gttgcccttt cgatggcatt
      301 gaaggtggca tcgacaggcc cgtctccgca ggcggcttct tcgtacacat catcattgca
      361 tctgattctg acggttgaag ttgaagttat gtcacttccg cttgatacct ggaagtattc
      421 catcctatac ttttcataag ccttccttga ttcctgggta atcaacgcct caatatccct
      481 gtccataata catttcttct tgtccgccaa atccttaaac tgtataaatg ccttgtttat
      541 ctcctcctgg ctaaggctgc tgtaacccat ttccttgagt ttttcttcga aggcatgcct
      601 tccggaatgc tttccgagta ccatcttact ctgcttcagc cctatggatt cgggagtcat
      661 tatttcatag gtctcactgt ttgacatgat tccatgctgg tgtatccccg attcgtgagc
      721 aaatgcatta acccctacaa tcgctttgtt gggctgcaca tgaacccccg ttgcaaagct
      781 gaccattgaa cttgttctgt aaagcttttc agtaactatt ccggtataaa cattataaaa
      841 atccttcctg gtcttaagtg ccatgacaat ctcttccaat gcggcgttcc ccgctctttc
      901 acccaatccg tttatagtgc attccacctg atctgcgccg ttttccaccg ctgccagaga
      961 atttgccaca gccattccca ggtcattatg gcagtgcacg ctgattattg ccttgtcaat
     1021 attgcttaca ttattcctga tactgcgtat aagccctccg aattcgacag gagttgtata
     1081 tcctactgta tcaggtatgt ttactactct tgctccggca ttgattgcct cctcaatcac
     1141 cctgtacaga aattccttat ccgtccttgc ggcatcttcg ggagaaaatt ccacttcagg
     1201 acacaggttc cttgcataag caaccatctc cgccgttttt tccagcacat cttccggctt
     1261 cattttcagc ttgtatttca tgtgaatgtc cgaggtggca atgaaggtgt gtatccttgg
     1321 ccgctccgca tattctattg cttcccacgc tgtattgata tcctgcctga cagcccttgc
     1381 cagggctgca attattgaac ttctgacatt ctttgctata gactttactc cttcataatc
     1441 accctttgaa gcggcgggga atccagcctc aatggcatca actcccagct cgcttaactg
     1501 ccttgctatc ttaagttttt cattactgtt taagcttatg cccggggatt gttccccgtc
     1561 ccttaatgtt gtatcaaaaa tatagatcct cttcgacata agaattcctc ccatacccct
     1621 attatttttt gttaatccac gacatcatct ttctgagctc ttttcctacc tgttccaact
     1681 ggtggctttg ctctttcttt ctcattgcgt tgaaggaagg tctgttaact tggttttcca
     1741 ataaccagtt ctttgcaaag gtgccgtctt gtacttcaga taatatcttt ctcatttctt
     1801 ttcttgtatc ttcagtgatt attcttttcc ctgaaacata atcaccatat tctgcagtat
     1861 cgcttattga atgcctcata tagccaaacc ctccctgaat tatcatgtct actatgagct
     1921 tcatttcatg caggcactca aaataggcat tttcaggtgc ataacctgct tccacaagag
     1981 tttcgaagcc cgccttcata agctcggtta cacctccgca aagcacagcc tgttctccaa
     2041 agaggtctgt ctcggtttca tccttgaagg ttgtttccag cactcctgct cttgtacctc
     2101 cgataccctt tgcatatgca agggcattta cctttgcgtt tccggaatag ttctgatgca
     2161 cagctatcag acatggaact ccttttccct cctgatactg acttcttaat agatgacccg
     2221 gactcttcgg tgctaccatg aatacatcaa tattttccgg tggaactatt tgcccataat
     2281 gtatattaaa cccgtgagca aacatcaatg actttccctc tctcagatta ggttctacag
     2341 attctttgta gatctcagcc gacttctcat caggtgtaac cattatgata aaatcgccct
     2401 ttttaacagc ctctgcgact tcatatactt caatgccatg agactctgca ctttcccaag
     2461 acttactgcc tttgtaaagg ccgacacata catcaactcc gctatccctt agatttagtg
     2521 catgtgcatg tccctggctt ccaaaaccta taacggaaac cttcttaccc tttagtacct
     2581 ctagatttgc atccttttcg taatacattt ttacatttga cattttacat cctcctaaaa
     2641 aattattaat ataactcaaa aagacttttc accccattac tggagcgaaa agtctttcgc
     2701 ggtaccatcc aattttcttc tctttttatt taacggctta gccggcgggg cttactgcta
     2761 tttcagcctg caactccagg atgaagactt tcaacaaatt catcctatgg attcacacca
     2821 gcctccactc tctgaaagaa ttaaaatctg tttacttatt cctgtcatcg tttttgattt
     2881 tattaagctg cttaatatct gtatcacgct aataattata attaggctta taatcagtcc
     2941 cacgacaaat agaattatgc ctgaaaccaa tagcacttgg ctgcttagca gcagtacaag
     3001 gactgataat aggataattc caaaaataat atatgaaatt gtgctgttaa accgtactct
     3061 gtccatattg atccctttca tcttcacaat ttcctttccg cctttaatcg gcagaaaaat
     3121 atttaaagaa aatgatacca gcttttaaag catatgtcaa atcttttttt attattatat
     3181 tattatatta tgggttgtgc tataccaaac ttctttgaaa gtagattcat tatatgttat
     3241 atttgttaca tagtattccg gtaccctgaa cacattatag ctgcaaggca ctattaagga
     3301 ggttttgctt atgaacaact ttactgatta tcttgatata aacagggagg tgcttgacgg
     3361 cctcagatat tacaagcccg ttgttgctct cgaatcaacc ataatttccc acgggatgcc
     3421 atatccggat aatattgaaa ccgctctgtc cctggaaaaa attatccgtg aaaacggagc
     3481 tataccggca accatgggaa taataaaggg aagaataaaa ataggccttg accgtgaaga
     3541 aatggaattc ctggcaaaag caaaggatat tatcaaaaca agcaggcgtg atattccctt
     3601 tgtggtagga atgggcttat ccggaggaac tacagttgca gctacaatga ttttatcgca
     3661 gtatgccgga attcgtgtct ttgccaccgg aggaatagga ggcgtacaca gaaacgcaga
     3721 aaagagcttt gacatttcag cagaccttac ggagctttca aatacaaatg ttgcagtgat
     3781 atgcgccggt ataaaggcaa tacttgatat cgggcttacc cttgaacggc ttgagacctt
     3841 cggagttccg gttatcggat accgtactga tgatttccct gccttttata ccagaagcag
     3901 cggatttaag atccccatga gagcaggcag cccacgggaa tgtgcgaaaa taatgaaaac
     3961 aaaatgggat ctgaatctgg acggaggaat tgtagtggca aatccgatcc cgaagcaata
     4021 tgaagctcag aaggataata ttgataaagc gatagaactg gctctttttg aggcagataa
     4081 aaacggcatt accggcaagg cagtaactcc gtacctgctg ggcaaggtca aggagataac
     4141 agccggagaa agccttgcag ccaatatagc tttggttagg aacaacgctt tgctggctgc
     4201 tcagatagca gtcgaataca gcaagctgga aaatggcagc taaagctgca gatacgagac
     4261 acgagatacg agattccggg gttacgggta acacagagga cacagatttc ccacggattc
     4321 ctgcggaata acacagcaac tattatattt aatatagtta cctcacggaa tttagatact
     4381 tacttaatta tcggtcatta tctatgttca aaattttcct ctgctgtgta tttagtggtt
     4441 ttctgtgttt ttagtgttaa ccgttaccaa ctacgcatgt ttctattatt caaatagctt
     4501 cctgatccct tcatcatagg gctgccttac aactcccttt tctgttatta tagccgtaat
     4561 cagctcatgg ggagtcacat caaaagccgg attgtaggtc tttaccccga gcggggcagt
     4621 ccttctgccc aggccgcatg tgatttcgtc aggcttcctc tcttcaatgg gaatctcatt
     4681 cccgcttgga gtttcaatat ctatagtcga tgtgggccct gctgtataaa atggtatatt
     4741 atgcgccttt gcaagtacgg caaggccata agtgccgatt ttatttgccg tatctccgtt
     4801 ggccgcgact ctgtcgcagc ctacaatcac cgcatctatc aaaccctttg ccattaccgc
     4861 tgcggccata ttgtcgcaga taagggttac atccactccg gcctgcataa gctcccaggc
     4921 agtaagcctt gccccctgaa gcagaggcct tgtctcatct gcaaatactt ttatatccca
     4981 acccttctcc tttgccagat atatcggagc cagggcagtt ccgtacatag ccgttgccag
     5041 gccccctgca ttgcaatggg taagtaccgt cattccatca tgaaacaggg taagtgcatt
     5101 ctcgcctatc gctctgcaca tactttcgtc ttcatccctt atggcatttg cttccctgat
     5161 aagtatttcc ttcaactctt ccacggtttt atttttattg tcaaatgctg ctctttccat
     5221 tctcctcaat gcccagaaaa gatttaccgc cgtaggcctt gaacttgcaa ggtattggga
     5281 attctttttt aattcagtca taaattcctc atggctttta gcccccgtat cccgcattgc
     5341 gacacacagc ccgtaagcag cggctattcc tatggcagga gcgcctctta ccttaagatg
     5401 aagtatagca tcccatacgt cctccgcctt gtccagcttt ataaattcag ttgtatcggg
     5461 gagctttgtc tgatccaata taatcagttc attgttctcc cattttacag acctcatggc
     5521 ttcaaagatt tttttcataa ggcactccct tgttgtgctg cttccacaac atgcttcaga
     5581 agcatcaatg ttgtaacagc acctaccccg cccggtacgg gggttatttt tgatgcaata
     5641 tccttcacct tttcaaagtc gacatccccg catatcccgc cttccggcgc cccattgact
     5701 ccaacatcta taacaacagc tccgggcttt atatagtcag cccctacggc ccttgctttt
     5761 ccaagggcag ccactacgat atccgcacgg gaaacaatgc tgcttatatc ctttgttctg
     5821 gaatggcata tggtcacagt ggcattctct ttaagcagca tcatgctcag aggctttccc
     5881 acaaccatgc ttctgccgat aacagccaca ttctccccct ggaggtttat tccgaaaaac
     5941 ctgataattt ccataaccgc cgcaggggtg cacggctcaa agcccgagtc gtcgccttca
     6001 aataccttgg ccagattgta aggattcata caatctatat ccttggcagg gtttatcata
     6061 ttccttgccc tatcctcgtc tatatgtgcc ggaagcggcc tgaacaccag tatcccattt
     6121 atgcttctgt cgctgtttgc ggcttcaagg gccttcatga agtcctcatt gcttatatca
     6181 tcagaaagcg ccgtcacttc acagcttatt cctatggact cacaattttt tattatcccc
     6241 ttttcataag aaatatcctc agggcgtgct cctactctga ttattcccag cttgggcttc
     6301 aatccttcag cggcaagaag ctgtactttt tttgccagtt cttccttaat gcttcttgca
     6361 gcctctctgc agtctaatac agccatatgt ccattactcc tttattaaaa tatattttat
     6421 ctaaattcat taaagcactc tatcattgcc actgcaatct taaatgcctg ttctctgctt
     6481 gccctgccgt agctgccctc ctgtccttct ttgggggcag gcccgaacat gttatttcca
     6541 atgccggtta tgatattgtt ttttaccatg aaatagacag aatccttggc atagccgctt
     6601 ataagttcgt cgtcactgaa tttggccact ccggccgtgt ccagaacatg ccggtcatag
     6661 gccgcgtctt ctcccaatgc ccaaccttcc cagtaaacgg ctttgtaaac tcttgtaagc
     6721 atggtgcagg cctgctctct ggtcaagctc gcatccgggg caaacagccc gtttccgatg
     6781 cccgacacaa ttcccaagct gtatgccttg attacgtcag aatcactggt atcggtgaat
     6841 ggactggcaa ggtcaactgc aatcttactg cccttaagat tctcataaag cttcactgcc
     6901 agggcgctga attccttcct ggtgatgccc ctggccatat cctgattcag gaggatatca
     6961 gggattatgg ataacctatc cgccttgtcc atccattcgc aggcccagtc tgaagtgttg
     7021 agccactttc cttctcttcc gtttttatat tgcataaaat cagggatttg atatccgaaa
     7081 gtccattcaa atgaaggctc cgcacctttt gtccatttga gatagcctcc cttctgcata
     7141 tcaacccatg cgttgagagc cgctgatatg ttgtcgtact gcctgcctcc tatgcttacg
     7201 ggagaagaga gctttcctgt agctgtattc actgccttgg aagtaaccgc agctttgtta
     7261 aaaaaaggat aagcaggaaa tgggctgaac ttggaatccc aatagcagta attatatatg
     7321 ccggtttttg cccccagcct tatattggca aaagcatctc catcctcttc attctttttc
     7381 cctgctttta atctgacagt accgctgaaa tagaagttat tggttggaac ctcctctcca
     7441 attcccataa cataaagttc acctttggaa tcggttgtgc ttgcatatat ccttcctctt
     7501 gaataggcgt tttctatagt tacttctctt cccggctttg ttatgccaat ggcaagttgt
     7561 atcctggcaa tttttgtttc cacattgcct tcatttccca catttttcag ctcggctatt
     7621 tccgactctc ccgcgatacc gcctgccata atctcttcat tcgcataagg gccggaatgc
     7681 tcaaaatata tactgccttt attcagagcc ttatccatgc cggagtccct cagttctccc
     7741 actatgcccc ctgccgcaga tcttgaactg ttgggagccc gtgtctgcat aacagtgaca
     7801 tttccctgat tcaacagatt ttctgcttct gcgccaaatc cgcagaatcc tgccaggccg
     7861 cctacggcac ctatgccgtc aaaagtcgcc gtaatatttc cctgattggt caggttgaaa
     7921 attccggcct cctttcccat tgcatctccg gttatacccc ccatatatag tctgtcacca
     7981 ccgcttattt ttatggtgag atcagctttg ttgatcatgg aatcaatcct gcccaaaagg
     8041 tgtcctgcag ctccgccaaa atagaaattc cccgggacgt taacggacaa atccacactt
     8101 ccttctgtta tgcagttttc aatgcttcct tccgcccagg ctgcaatcaa accgaagcag
     8161 gcctcttctt cagatgatat ggccttactg atctgcccgc tgaccgtaag atttttgatg
     8221 gaggatctcc tgtccgtacc gccgaacaag cccatattac ggctgtcggc aatatttctc
     8281 aaagttattg tttttccatt accgtcaaaa gtgccgctaa aagaaagccc gttgacgccg
     8341 acagggttcc atgtgttttc gcccatatct atgtttccgg tgagcttata ggatgcctcc
     8401 atataaccgg cattccatcc aacctcagaa aaagccaaaa ggtcttctgc cgtggatata
     8461 aggtagggat cctccggagt tcccgcgcct ccgctgaagg aaggcatttt gccgggttcc
     8521 tcccggcagt atgcaggaat caggcttccc ccaatgaaaa gaacaatcac gattaaaaat
     8581 ataagctttc tgaatcggtt tttgctttta ttcatctctt accctcctca ctttccgatc
     8641 cctttgggcc ctttgaagcc cttacagcat gactttatac cattgcagta aaattttatg
     8701 atataatatt ctcaagcgca tattataata ttattctact atatcactat tttttttacc
     8761 aggaggggtt aaattgaata cggacagaat gatcagtgaa tttattgaaa tggccaaggt
     8821 aagcagcctg tcgcttaagg aaggcagatt tgcttcgctg ttggctgaaa aactcagggg
     8881 tttggatttc gaggtatata tagacaaagc aggtgagctg gcgggaggag ataccggaaa
     8941 tgtactgggc aggttaaagg ggactcttga cactcagccg atactcttat gtgcccatat
     9001 ggatacggta gtcccgggag aaaacataaa ccctataatc cgtgatggaa tagtatacag
     9061 cgatgggaca agcatactgg gaggcgatga caaggcagga attgctgcga tattggaagc
     9121 agtaagatac ataaaagaga ataacatacc tcacggtgac attgaggttg cattcacaat
     9181 ttgcgaagaa aacggaatgt tcggtgccaa gcatctcgat tacagctggc tcaggtctga
     9241 atttgccttc gttcttgaca gcagcggagc cgtgggatca gtaattgtac aggccccggc
     9301 acagattaaa accctttcgg taataaaggg aaaagctgcc catgccggat tggctccgga
     9361 aaaaggcatc agcgcaatac aggcagctgc aagagctgtt aacaatatga agcttctccg
     9421 catagacgaa gagacaaccg caaatatagg caggataaac ggcggcggtg ccaccaacat
     9481 tgtatgtgac aaagttgaaa tggaaatgga agcaagaagc ttgacaagga gtaaagtcga
     9541 agcccaggca aagcatatgc tggagtgcat agatgatgcc tgcagggaat tcggtgcaga
     9601 acatgaaaca acacagcagc tgagctacac cgagttaaac attccgaagg atgcggaagt
     9661 aataaagctt gtggactcgg caataaaaag aataggcctt gaaacccgtc tggagtcaac
     9721 aggaggcggc agtgacacaa atataatgag cggcaaaggg atacaggcag taactctggg
     9781 aataggaatg accaatgcgc acactacttc ggaatacata gcaataagcg atatggagaa
     9841 aaccgcggaa ttagtcgcat caataataca agaggctcgt tagagcctct tgtcatttat
     9901 aagcttttcc actatgggca gatctgccgg tacaaaatca aagcccggaa gttcatcctt
     9961 cgttacccat ctaaagtcat tgcattcaat tgcttttaga tcacctttga ttatcctgca
    10021 taggtagcac agaagcaata tgtctttttc ttcatatctg aacttcacaa ccttatagat
    10081 atcatcaact tcaatatcta tatccatttc ttccttaatt tctcttatga tgcactcttc
    10141 gggagtttca tcgggctcca gcttcccccc gggaaactcc cacttgtatt ccatgtgaga
    10201 gccctgctgc cgctgtgcaa taagcacttt cccatccctg attatggccg ctgccgttac
    10261 aatatacata ttacaccttc ggtccggcgt tgactatatc ttcagacaca cccctgaatt
    10321 tccggaagtt cgcattgaaa tccgctgcaa gcttcagagc tgtcttttca tattccgcct
    10381 tatcctgcca cacattcacc ggcttcagaa cctcagccgg aactcctggg cattcggcag
    10441 gcacagccaa tccgaatatc gggtcggtat cgtagccgac attatccaac tccccgctta
    10501 ttgcggcatt taccattgct ctggtgtagg acaatttcat gcgcttccct actccgtagg
    10561 agcctccgct ccagccggta ttgacaagga atacccttgc gttgttcttg tcgatttttt
    10621 tacccagaag ctctgcataa acctgcggag gcatcggcat aaaaggtgct ccgaagcaag
    10681 tggagaatgt tgcggtaggc tctattatcc ctctttccgt tcctgccagc ttgcttgtat
    10741 acccggacat gaaatggtac attgcctgct cttttgtaag ccttgcaatc ggaggcagca
    10801 cgccggtagc atcagccgta aggaatatta ccgtagaagg attgcctccc actccttctt
    10861 caacagcatt atcaatataa gttacaggat atcccgcacg ggtattctcg gtgaactttt
    10921 cactgtcata gtccggaacc cctgtctcag gatccagcac aagattctcc gttaccgcac
    10981 caaaccttat ggcattccat atctgcggct cgttttcctt tgaaagcctt atgcattttg
    11041 cataacagcc tccctcgaag ttgaataccc cgtcctctgt ccagccgtgc tcgtcatcac
    11101 caataagcct tcttcccttg tcagcggaca aagtagtctt ccctgtaccc gacaatccga
    11161 agaacagcgc aacatcaccg tcttctccga tgttggccga acaatgcata ggcattactc
    11221 ccttcattgg aagtataaag ttcataatcg taaaaatgga cttctttatc tcgccgcagt
    11281 acattgatcc gcctataata acaattttct ttgcaaaatt cagtataata aaggcttcgg
    11341 aattcacacc gtcagcttcg gcatcggcat gacagcccgg tgcggctata accgtgtatt
    11401 caggctcaaa accttctaac tcgccttcct ttggcttgat gaaaagattc tgggcaaaaa
    11461 gattctggct tgcatattca tttatgatcc ttatcggcat cctataattc ttgtcagccc
    11521 cgacaaaacc gtcaaataca aaaagctcct tgtcttttat atattcatta attttattat
    11581 acaggttttc aaatttctca ccgcttatgg gtacgttgac ctttccccaa tccaccttgt
    11641 cgtgtatgcc gggatcatca actataaatc tgtcgcccgg ggatctgcct gtatattttc
    11701 ctgtgttgat gctcaaagct cctgtatcgg taagagcagc cccttctttt ttcagggcaa
    11761 tttccacaag cttccctaca gtaagattgc gatgcacctt tgcaccaaga ttatccaaat
    11821 tcatttcggc acctccaagt ataaaacaaa tatctatgta tgctgcaatc tgaattgcaa
    11881 ataatgcaaa aactcagcca taagcatatc ataacctata ttatatatca ttattgaaat
    11941 ttgcgccatg aattttgaat acaaaaagtg atagatttca atcctctatt cccgttttca
    12001 gtatttcata gaacttatcc atatcaaggt ttgaccttac tgtctcagca agcttgtcgt
    12061 actggccttc cttatacacc caataatcag gagccaggtt ttccaaagga accaatccct
    12121 tccttctccg gatcagatta agaaagcttc ttgtgaactc cgaactgtca aatatgccgt
    12181 ggatatatgt gccgaatacc gtaagatcct tatttatcat tccgtcccgt ccaaaaactc
    12241 cctttccccc tctttcgctt atggttataa aggagttttc cgcatctgtg cccgcagttt
    12301 ctcccatatg tatttcgtag cctcttactt cagctccgaa tatattgtcc ctgccctttg
    12361 aaagagtcgt aaatttgttc tttctgaact ccgtaacggc gttaaaaagg cccaagcctt
    12421 ccgagcgttc attgtccgac tcggccttaa atttatccga tatttctttc ccaagcatct
    12481 ggtaaccccc gcagattcca aaaacaacgc tgcccctgga atggcatttc agtattttct
    12541 tgtccatccc cgtatttttc agtgcaagca tgtcatgaat agtgcttttt gtgcccggca
    12601 gtattataac atccggctcc ccgagttctt tgtcaatttc cgcaaatctc agggatacat
    12661 cctcatgaag ccttaaggcg ttgaaatcag tatgattcga catataaggc agccttatta
    12721 ccgcaatatc cagctctccg cccttctccg gcccggactt cagccattcg gaaacgctgt
    12781 cctcatcctc caggttcatg ctgaaatacg gtattacccc cagcaccggc acattaataa
    12841 tttcttccag catcttgatt ccgggcttca atatctcctg gctgcccctg aatttattta
    12901 ttattacacc ttttacaagc tttttttctt ccgggcttaa aagcagcatt gtaccggcca
    12961 gggatgcaaa tacaccgccc ctgtctatat ctcccacaag cagtactgga gccttggcaa
    13021 ttttggccat acccatgttt acgaaatcct catgattaag gtttatctct gcagggcttc
    13081 ctgccccttc tatgacaaca atatcacttc tgtcctccag cctccggtaa atgtctgaaa
    13141 tcctctcctt aagccttggc ttgaagttaa aatactcaac ggcatccatg tcagcataaa
    13201 caccgccttc aattataacc tgggacttcc tgtccgacac aggcttcaga agtataggat
    13261 tcatgcaagc ttcgggcagc ttcccgcagg cctccgcctg catcacctga gcccttccca
    13321 tttcgtgccc ttctattgtt atatatgaat ttaaagacat attctgagat ttaaagggat
    13381 tcacattata tccatcccgg gcaagtatcc tgcagattgc agtgcacatc aggctttttc
    13441 ccaccgagga tgcggttccc tgtatcatca ggctcttaga cttcaattcc cttttcgcca
    13501 tgttaattcc ctttccgcag cattgcttgg tacaacgcag tattgcgccg cttatagcag
    13561 ttcatacccc gctgaagaat ggctagaagc aagcaagaca aggcgtgcaa ggcggaatga
    13621 gcggagcgta cttattgtcg tacgtgagca cattccgacc gcagcacaac gcagtattgc
    13681 gccgcttata gcagttcata ccccgctgaa gaatggctag aagcaagcaa gacaaggcgt
    13741 gcaaggcgga atgagcggag cgtacatgtc agtacgtgag gacgtttcgg ccgcagcaca
    13801 acgcagtatt gcgccgctta tagacattct ttacctgccg ctgttgattc ttcgtataat
    13861 acagcgtccc tcttcattaa tctcataaag gctgtatctt ccctgctccg cagtaaagct
    13921 ccagtatgca tcttccgtaa gcccgagaag caccaggggt attatcctca tggtgccctg
    13981 atgggataca atcagcacct tttttcccct gtactccttc aaaatatcct caatactgct
    14041 ctttacacgg atatatattt caaagaagct ctctcctccg ggaggggctg attttctcca
    14101 atcgctgccc cacttttcca gggcttcctt gtacctcacc ttcaattccc tgtggtgctt
    14161 tccttcccag tcaccaaagt tcagttccct cagccggtca tccttaataa tcatatcggg
    14221 ggtatatccg cttacaatgg aagcggtttc aaccgccctt atcaggctgc ttgaaattac
    14281 ggcatcaaat tgcacatcgt gaagtatatc agccaggtct tccgcctgca ttatcccctt
    14341 ttcactgagt cccacatcgg tccagccgta atatacccct tttatgttca tgtcggtttc
    14401 accatgtctt attaaatata tttcagtctt gctgcctttc atatgattcc ccttcctgcc
    14461 atgactgccg ttataaggat gaaggacact tccaagatct catttgctgc tcccagggta
    14521 tcccccgtca tcccctgcag cttggaatat ataaagcttc tgtacaggaa gcatataacc
    14581 gtacaaatgc caaaaacaac caaaccccaa atatgaccaa agcccagaat tataataata
    14641 cctaaagcag ctgccagcac cgtcgatctc atggtctgct tttcaagata aaggcctccc
    14701 attcctgaac ctgagcgggc atatacagag accttcataa gaagcaccag gagtgttctt
    14761 gccgcaacag gggacaaaag caccgccccg tacagcgcct ttcccgtcag catggcaagc
    14821 agtgacattc tcagcagata atcaaaaatg atggctatag tcccattggt gcctatcctg
    14881 ctgtctctca ttatctcaag cattctgtcc ctgtcccgtg atgagaaaat gccgtcacag
    14941 gtgtccgcaa ggccatccgc atggaggcct cccgtaatgg cagtacctgc aataaggcag
    15001 catatcacgg ggaatactcc tcccaagatg tttgaggcaa ttattagtat cgtcagatta
    15061 aatgcaccta taatcaaccc gacaatagga aaaaaagcaa ctccctttgc aaaatcctct
    15121 tcagttgcct tcaaattctt atttacagat attctggtca agaactgcag ggtgattaaa
    15181 aaaatcctca tatccgttcc tccatatcac ttaagcttca gggggattcc gcatacagtg
    15241 atataaactt catcgcaccg ttcagctatg tattggttcg tccttccggc aatatccctg
    15301 aatgccctcg ccaaggggtt ttcaggtaca atcccgcttc ccagctcatt agtgacaatt
    15361 ataacggaag cctctgaatt tccgataccc gccataagct ccgcaatctc tctttctatt
    15421 tccttttccg ccttttcaaa atcctcattt gaacaatcat tgccttccat accggtatat
    15481 tccaggagaa ggttggtcac cattacagta atgcagtcaa gcaatatccc tttatattgg
    15541 ctgccctttt cagatatcac tcttcccagc cctttacagc cttcaaaggt atcccattcc
    15601 cggggccttg attccttatg cctttttacc ctcagtttca tttcctcgtc aaagggtatt
    15661 gacgtggcta tgtacagaac atttccccca agctcctttg ccagcttttc cgcgtaactg
    15721 ctcttgccgc tccgggcccc gcccgttatt aatatagatt tacccataaa ccaattatct
    15781 ccttatcagt ataagtattc atacacaaac ttataaatgg ctagaagcaa gcaagacaag
    15841 gcgtgcaagg tgaaatgccc ggagcgtaca ttaagataca tgaggacatt gaatccgaag
    15901 cataacgcag tattgcgccg cttatcgcca tttattttct tatatctatc aagaagtcat
    15961 ccttaatcgt cgcttcctca aaggttgcca tatcggatat gattgcttcc gccgcctcta
    16021 ttatattgaa ggcaaggggg cagccgctgc cctctcccag cctcatatta agatccagca
    16081 aaggctcaag cccaatttca ttgattacca ggctgtaccc gggttccgcc gatagatggg
    16141 acggaataat gtaatccctt accagggggt tgatcctata tgctgccagg gcagctgccg
    16201 ccgaaataaa accgtctata acaatgggca ccctgcaata tgccgctcca aggaagcatc
    16261 ctgtcatacc tgcaatatca aagcctccga cttttgaaag cacatcgatg ggatcataag
    16321 gatccggctt gtttatgctt attgccctct caattacaga cttcttactg gcatagccct
    16381 cttccgtcaa tcctacgccc ttgcccaccg cctcttccgc gctgcagcca aggaaggcca
    16441 ttgcaacagc acttgccgtg cttgtatttc cgattcccat ttcaccggtt ccaaacaggt
    16501 tgtatccttc ctttacaagc tgtgaaacca tttcaatccc tgtctctatg gcagatatcg
    16561 cttcttcctt gctcatggca gggccttttg ccatattgga ggttcctttt cgtacttttc
    16621 tgtttatcaa gcccggatat tccaaatcgc ttccgactcc gatatctaca actctgatat
    16681 cagctcccgc atgcttagac agcacgcata ctcctgtaat acccttcaga aaattaattg
    16741 tcattaatgc ggtaactgct tgaggtgcgg cacttacccc ttcctccgcc acaccgttgt
    16801 ctgacgccat tataattgta acctttttct caaaactgtt cttttgtttt ccggttattc
    16861 cggaaatctt taccgcaatg tcctccagct ttcccagact gccaatgggc tttacaagct
    16921 tgtcaagcct ttccgccgct tccttcttac tttcttcata tgagggcctg attcctgccg
    16981 ctgtcctttc aaaaatctcc ataatgcatc tccttaatca gtatgaaata aaaaaacccg
    17041 ctgtccaact taatcgagcc ggagcggaaa atattaactt aataatcctc ctcccctccc
    17101 tccgaaggta gaaaatgtat cataggcagg tttcctggct tgtggctctc cttactctcc
    17161 ggccttctcg cactacaatg gcttgtcgga tttcataccc acttacagta gcgggggctg
    17221 cagcggcttt gaaccgcttt cccttttaac ccgttagggc accatatgat tgtattcgat
    17281 tgacatattc attatatatt ttaagaatta tccagtcaat gcataatatg cattttataa
    17341 aaccaaccgg cattaaaaat gcgtgttaca acgcattttt ttcgtttaat tcacccaaat
    17401 ttaatatttt tagacttagc tgtcacatta tttgtgcagt ttaatattat gatatcagag
    17461 gtatatgtag gagacaacat cagacaccgg tacatctcct gcaaatagat ttatagctca
    17521 gtagcaatgc aggaaatcaa atctattatc ctcaaatatt taggaggtga attaaatggg
    17581 caaagagcac agacgcgata aagaattcag aataaagcac tttccggata acataaggga
    17641 cttgctgtgc ctgctggcag ataatgaaga caacagggct actataatac tcaatacttg
    17701 tgatgattgt gttcaggaaa atgtagaagt agttgcagta ataggaaatc ttgcagtctt
    17761 ccgctgccat gacaaattca aattcgtaga tatccactgc atttgcgaag taatcgttga
    17821 ctgtgagaca atattaagtg aactccttga atcaaagagt gaatgcaaat gttagcaaat
    17881 aggagggact cccataatta gggaggccct cttattcctt tcttaccacc aggaaggccg
    17941 tttgcctgcc ctcaggtctg cagccttcaa acctccggtg tattcaaagt gaggcttgtc
    18001 aactatggac ttccaatctc cgccccatgt cagtcccagc tttttgccct ctattccgat
    18061 aatcctccaa tagcggttat tattccatac ggcttttcct ttgacaatcg gtaccacatc
    18121 ataagcaagc ccgaagccat gagggcctat cagccgcata tttgttacta tattgccggg
    18181 ccttgtacgg ccttgtgaat acagatactc ctgatattcc gaatctctca gggtatttgt
    18241 cacaattatt tggactccct gcctcttgca agcttcgata tgttttctgc atatttcctg
    18301 tactaccggg tgaagatcct ctattttacg gctattagtc acttttttca ccacctgata
    18361 tatgaattgg agcatgtaca cctcctactt catatatatg agaataaaaa agttttgcaa
    18421 tcaatatgac tgcaaaaccc ttaagtttat gccggatcta cttatctgat accagtgccg
    18481 ccactctcaa aatatcctct gcggatttct caatatcatc gtaggtggtc ttatatgagc
    18541 atacgcttat cctcataacc gatttccccg accatttcga acctcccagc cagcagacgc
    18601 cggattcctg gactccttta ataagggctt ccgttttccg gtcattatcg taataaacca
    18661 atacctggtt gtagactaca tcgttcaaaa tctgaagccc gccttcctca agcagccttg
    18721 aaaaatacac agccttgcaa tgaagctctt caaccagctc agctgcgcct ttcctgccca
    18781 gcccttttaa tgttgcccac aaatctattg ctctcgccct tcttgacatc tcgggagtat
    18841 atagcattcc atccctgttt tcattatata ttatgtagga ccctgtcata tgcattgcat
    18901 ttaccagtgc atccttatgc ctgcaaagaa caatcccgtt atcataagga gcatttaagg
    18961 tcttatgggc gtctaccgac caggaatcag ccaactccat acccttagtc aggcggttca
    19021 ttttttcgca ggcagctgcc cataatccga aggcaccgtc aatatgcacc catgcgccgg
    19081 cagcattggc cttcctgcaa acagaatcaa aatcatcaaa agcccctgta ttgacatttc
    19141 cggcttggag tatcaataat gtcctgttgt ccggctcagg aatttcatca gctttaatgc
    19201 gcccctgtcc gtcagtggga accctgataa gctttgcgtt tcccaggcct attatggaca
    19261 acgccttaaa taccgttgaa tgggcctctt cacctacaac tacctttatc tggggagcat
    19321 tgaaaagccc ctcttttgtg acatcatatc ccagattgcc aagcagataa ttcctccctg
    19381 ccagcaggcc gcaaatagtg gctgtggaac tgccgctgac aaagcccgca gctgtatcat
    19441 cgggtaattt taaaagctgt tttaaccagc tctcgcatac ctcttcaagc tttgaagcaa
    19501 taggagacaa tacaaacagg gctgaattct ggtcccaagc atcagaaagc cactttgagc
    19561 ataatgccga gggaagaatg ccgccgcata caaatccgaa atacctcccc cccgtctggg
    19621 cgactgttgc aggagaaccg tatttaccca acatgttcaa tatatttttg gtatcctccg
    19681 gctcctctga caaggcttcc tcaaacactg acagattacc gacagccttt ttatcgggga
    19741 aaacccgcat gtcattgacc tgcttcacat atcttaaggc caggtcatgg gctaatacca
    19801 tatcctcctc aaaggtttgc tttctgattc tgtccaaaat cggactgaca tagttttttt
    19861 ctttcatatg aagggtgcct ccttataatt tttagtgtat atacacactt ttgccaaaaa
    19921 gttattattc cacaagctgg gctaatattt ccgacagctg ttccagtttt tcctttccta
    19981 ctttctgctc cagctctgcc tgtgccgttc tccagagcac ttccccctga ttaaggacct
    20041 gctgtcccgc tcccgtaacc tttagcagac ggctgcgccg gcccttttcg gaaacgtctt
    20101 cgatatattt cagcttaaaa agcggtttca gagagcgtac aagggttgtt cgttccagcc
    20161 ccacatagcc tgccagatca ctgacgctgc aagccttcaa cctgcctata tttattaaaa
    20221 gggaatattg gctgacggta agccctatag gcagtaaaac cctgttgtat aagtccgtta
    20281 cggcattagc agcccgtctt aagttaatac aatagcaaac gctcttatgc cttttaatca
    20341 taagtatagt attccttccg tttatagtgt atatacacta tattaatata catttactgt
    20401 tttgtcaatg tccaggtgtt atgggaaatt taaaggaggg tatcccgccc tccttcagta
    20461 ttgcatattt atatctgtta tattatgcct tgatttgagt caaaatagag catatcagtt
    20521 taaatcactt cattgtcaag tttaccttct acttaacatt tttttgtgta tttcagcatc
    20581 tgtacaagat attctcctga aaggtcatct ttttcatccc tgtcaagctt tcttttgcca
    20641 aattcaacaa acccattaga cgagtagaaa tttttcaatt tctcattatc ttcacattca
    20701 agataaacaa ctttgccacc gagcacaaat tgtgcttcag caattttatc gcaagccatc
    20761 tttaatagct cgtccccgga tatcaaattc tgttcattgt atttgtaatt tttcccaatt
    20821 tgagctatca atggcgctga aaaaacatat ttctttatca ccggattgta tgtgccaaac
    20881 cttccgattt ttctttttac atttcctctc aatacagctt tatcaacaag aatggttttg
    20941 tttgccaaag taaaatatcc aaccagggta ggctcgtcct tatatgaggt aaaaactaga
    21001 aacgtttggg aaagagattg ctttgcaaac tcgattgcct tatggtgcag aaacttttct
    21061 acatcagaat taagcggaca tgaaaaaccg agaagaattt ttattgtttc atcctccccg
    21121 atttcatcta aaatatgttc caggtgtacg attaaatacc ctttaattgt actttctgca
    21181 ctcacttacg gcctccgaat atatttttta cttggcttct agttgctgta gtgcatgtct
    21241 tactaaaatt aacttcttta cttgtcttgt tctccgcatt ttctaaagcc gacgcaaaag
    21301 cacgacccaa acgctgattt ttgatgtcaa tgttttttaa aatgcttttt gtagccatat
    21361 gacatccctc ttcctatatc attacaatca attatattgt ttactcttgt atttacaaca
    21421 cagtaacatc ctatgtttat agtataaccc aaagtttgaa tttgttcaag gtttgactaa
    21481 attaatcact tcactacata gtttatcggt tgtgttctac aattctaata tatattatga
    21541 taaaaaacaa tatattccac tatatttcat actgatacta taaaaaaaag agggctgccg
    21601 ccctccttca gctatctctt atgctttata
//
LOCUS       DAOOAB010000042        21506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3229_length_21506_cov_15.800615, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000042 DAOOAB010000000
VERSION     DAOOAB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_21506_cov_15.800615"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..678
                     /locus_tag="PKU88_09245"
     CDS             76..678
                     /locus_tag="PKU88_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX69498.1"
                     /translation="MNGYERRTELKKDKIRAAALELFCMYGTDKTSINEIAQKAGVAP
                     ASIYNYFGSKEGLMKDTTINLLENGWRARKELWETDLPFPELVKRAISMNYDFIDHIN
                     LDTLSALFNTDPEIKKLADDYYKHKYPYIVSQFIEKGRREGYIRKDISVEAATIYLKM
                     YQDLFQQPEILRNRNKDLFKELCDLMLYGLAGQPITSKTE"
     gene            845..1849
                     /locus_tag="PKU88_09250"
     CDS             845..1849
                     /locus_tag="PKU88_09250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPX69499.1"
                     /translation="MKKYLMLGLLSLLIMVSDQPKQSEESIKIVLGGDVLLDGSVGWL
                     IENQGCSSIWGDMISILTSADFSMVNLENPLSSRGSMEKDKQFTFRGKPAYVKALESA
                     GIDMVSLANNHVLDYGKTALLDTMKHLDDAGILYAGAGQNEDAASMPVFIDKGAVSMA
                     VLCSSHVIPFVHWHAGKNKPGVASAYDPARLISEVASASEKADIVLVYLHWGKEMKSQ
                     PVQYQKNIARMLIDAGADLVIGSHPHIIQGLEFYKGKLIAYSLGNLVFTNSFKETMLL
                     SIRLSKQEMLEAHVIPCMIKSSKTMLVKDEQKKQDFIDKLNSLSPGVQVHKDGRITPD
                     "
     gene            1987..3363
                     /locus_tag="PKU88_09255"
     CDS             1987..3363
                     /locus_tag="PKU88_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX69500.1"
                     /translation="MEYVNPLGKEKVSKLLAEYSVPAIVGMIVNSIYNIVDRIFIGNS
                     SDLGTFGLAGITIGFPIMIILLSMGLLFGVGGATLFSIKLGEGQSEEADRALGNAFSL
                     MVIAGALFMITGQILLRPLLTLFGASEAVLPYSMEYMRVIFFGAIFQVIGMGMNNFIR
                     ADGSPRTAMLTMFLGAGLNTLLDPLFIYVFKMGMTGAALATILAQCISATWVVMYFIG
                     KHSKHKLKLKYMKLKLNVVLTITSLGIPGFALQLANSLLNAILNKSLLIYGGDIAVSG
                     MGIINSLQTIFYMPIIGIKQGVQPIISFNYGARKFDRVKEAVKLAVISATAIVVFGYV
                     ITRLFPRQIISIFNRDPDLIEFGSYGITAWFLCMPVVGFQIITSHFFQAIGKSKAAMF
                     LTLTRQVIFLIPALIIFPKFWGISGLLHAAPFADFFSALLTGIWFYYGNKNLEKYPLA
                     TISNNSIK"
     gene            3545..3763
                     /locus_tag="PKU88_09260"
     CDS             3545..3763
                     /locus_tag="PKU88_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69501.1"
                     /translation="MRNLSDAEVLSLTALFKMEQDSLTVSRALHALISNDELKKQSEA
                     GMLAAEGRVKGIRQFIVENGITNIQEVQ"
     gene            3765..4058
                     /locus_tag="PKU88_09265"
     CDS             3765..4058
                     /locus_tag="PKU88_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HPX69502.1"
                     /translation="MASMIGNIVKNNTDITDEVILTSMLGSLKAIAAAYFTATMTSTT
                     PELKSMFSSALVQVMNSHSVLTELAVKHGWEKPYVSPAEQLADVYKYSITEKQ"
     gene            complement(4169..4360)
                     /locus_tag="PKU88_09270"
     CDS             complement(4169..4360)
                     /locus_tag="PKU88_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69503.1"
                     /translation="MNLGEFKVVCCQCKSDNILEKYSRNRLEWVGGIIKYGEGIQRKC
                     CECDNESFMIFRTWEEKSP"
     gene            complement(4357..5457)
                     /locus_tag="PKU88_09275"
     CDS             complement(4357..5457)
                     /locus_tag="PKU88_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPX69504.1"
                     /translation="MKVAIIGAGTSGLSCAIELERNGIYPVIFERNEFIGEYHPHVSA
                     LLGLITRPASDPVKYLDKVLGIKLKPLSQFRKVIHYSPNNQAAVTGPLGHFLIRGKEE
                     NSVKNQLRSQVKSQIIFNSFVQPEDLENEFDYIVVADGHWSVPARYGIWQEVMRTWVM
                     GGIFEGDFEEDTLQMWLDNELTKGVYIYSAPYSKNKGVIAHVVQSIGQEELNGYWHRF
                     LQSRDILKKYDMLETWKLPHRAGSVTTNRVGKIYFAGVAGGGIEPFLGFGQFNAVVSG
                     VMIARSIARGIDVNLLLKDIHKKSRELSALRPMLNAADNKDFDRMMTIMKAPGLRSLV
                     YRTNIDVIKMLSTGLRLAASEKKIASQIKGGK"
     gene            5685..7010
                     /locus_tag="PKU88_09280"
     CDS             5685..7010
                     /locus_tag="PKU88_09280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX69505.1"
                     /translation="MKKISGKKSRLYIPVSIKFWLSHLFAILWTSLSIYISLPWLEDF
                     SEVFTFPAAFIIISGIAYFPGYLNAFLFASLMLDRQPPFKNEYPARDITVLIAARNEG
                     ERIADTIYYIAKQDYTGNIKVLIIDNGSEDDTAVNALKAGEAHNLDLSVIREEIPGKF
                     NALNAGLEHTSTDLIITLDADTLLHRSAIRYLAARMESAPKEVCAVAGSVLARNSRNA
                     LLAKIQEWDYFLGIASIKRLQGLYQGTLVAQGAYSIYKAESIREVGGWPDAIGEDIVL
                     TWNLLKKSGPIYFEPLAVAFTDVPTTIRSFSKQRSRWARGMIEALKQVKPWQQPLPYT
                     KYLTGMNLIMPFLDFCYTFFWLPGLILAFFGYYWIVGPMTLLVIPLTLMNYSILYAYQ
                     LYVFRRLNLRVRKNMRGFILFIIVYQMIMSPVSVWGYLQEFLKLKRNWK"
     gene            7121..8614
                     /locus_tag="PKU88_09285"
     CDS             7121..8614
                     /locus_tag="PKU88_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPX69506.1"
                     /translation="MPAINEEDSLYNKTADVSSDNIRSILGSNADFVSRVILVRNKPE
                     IPSVLVYMDGLVDISLINDNILKPLSQEEQFDSCSGEEDAARLISMGKIYSSSMKVRT
                     NMKDLTDDILTGSAALIFNDIRTAFTFEAKASLSRTISEPTGETVIKGAKDSFIEDLR
                     TNTALCRKKLTSPYLTIEQIRLGKQTKSNIAILYVKNIAKSKLIDEVKQRLESIDIDR
                     VLDAGVIEGAIVDDKYSAFPQIQYTERIDRLCASLVEGRAGILIEGLPTAYIVPGTLL
                     QFLQAPDDYSFQFIIASIVRFIRFMSMFITLVLPGFYISITSFHPEMLPAELAFSIVA
                     SRVGVPFPMFVEVLILLLAFELLIEAGVRLPNVIGQTVSIVGGLVVGQAAVEAKLVSP
                     ATVMIIAITAIAGFLMPNQDFSNAIRLWRFILAIFGSITGIFGLTFGLIFLLYHWCRI
                     ETFGIPYLDPFVANEDEQLQDTIFRLPLFTMKKRPSSLDPKNKKRME"
     gene            8622..9809
                     /locus_tag="PKU88_09290"
     CDS             8622..9809
                     /locus_tag="PKU88_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPX69507.1"
                     /translation="MRKYAILTVLLALTAALSGCMQIIMGRSEIDKLFIIRMFSFDEA
                     EEGKIKVTATTKDLSTGEGGGEVLQKGESIAAEGETVFDAVRNMSTYADRKLNAGHTE
                     FILFGESLARKGILPYLDLISRHPEFRYNAKLYVVKGGTANSLAEKASTSKYFVGDRL
                     AHIEENIGRTSISGTVTLNEVLLIFDERYLDTFIPYIEPVPTKATGEKPDTCDMLLNG
                     YAIFREDKLLYFASREESRGINWIMNRIGSGIIVLKDQTGQEISMIIVKGASRVIPEI
                     VGNELRCRVEISFSTNIGEIMGGRSVFDKASLDYITEQQENSIKREVENAIRIAQDKN
                     SDHFSIISKFRIKYPLLRDYFQQNWQELFPDIKFEVSVDSLIDGTYMINEPVTASKEA
                     VGE"
     gene            9806..10009
                     /locus_tag="PKU88_09295"
     CDS             9806..10009
                     /locus_tag="PKU88_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69508.1"
                     /translation="MKIFLMVVIVVIAITAEMIPVIKKKQHKEAAVLIMLGAVTLAYG
                     YYYITHVHTASLANLMFDIFRLR"
     gene            10022..11122
                     /locus_tag="PKU88_09300"
     CDS             10022..11122
                     /locus_tag="PKU88_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPX69509.1"
                     /translation="MKTGKDKISVKQLFFVFTIMVSSPTTRLLPKYAAAKAEQAGWVS
                     PIISTVPFILLILAIDSLLKKYKGQSMSDIISNILGRFLGKLVLVVYLLWALWLAAMY
                     TRYYSERLTSSIYPNISVGLFIILALIPIAYILRSGFTPIARMSEILLPFIGAMLIML
                     ALFLFPKVRADNLLPVYFNDIIPIFRGSIAVTGILSYLFLMFFLSDNVVNLKSLRVFG
                     YIAAYVNISSIMLVNFIVIGVMSSSLARRVSVPVLTVVKQITIMDIIENIEAVVVAIW
                     IFADFTMITTLLVIVLNLIKSIFNLSDTRLLINMLAVLVYFLSMGVCARRFELEDFTN
                     SLLMPINITLGYVFPFVLFALSKLKKTTNPSR"
     gene            complement(11160..11624)
                     /locus_tag="PKU88_09305"
     CDS             complement(11160..11624)
                     /locus_tag="PKU88_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HPX69510.1"
                     /translation="MNNLTQKEKGLLQDQKTHEEICIKKYNEYAGKAQDPQLKQLFSS
                     HAKHEQQHLNTINQLLSGQMPGMNQNQQQGRQGAQGQQPIRTGTGSKSDGDLCNDILM
                     TEKYVSGAYDTAIFEFRDPNIRQTLNHIQKEEQQHGESVFKYMESMGLYNPQ"
     gene            complement(11670..16478)
                     /locus_tag="PKU88_09310"
     CDS             complement(11670..16478)
                     /locus_tag="PKU88_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69511.1"
                     /translation="MVKKFVTVITIFALVLSAILPVQVIADDDIGYEGRICRDLGILK
                     GHTGIVDEEYLETRPSRLQAAIMFLRLKGLEQDALDYYGGKNFKDAGVVAWEEGRNVL
                     SYLKNHPELGWIGDGVNFMPYSLIDSRAYYKVLLESLGYKQKIDGKGDFDWDEVLDFA
                     EEKGLYKVAGIRNFTVKSLAIATVEALNTKMKGERRTLIEYLVDIGDVDKWDAISAGL
                     YSKELEAEVKDVRAISNSKVEVVFKESIDGTDAEDEDLYEIKHLDINKVSMKNEYAVI
                     IDTSAMKENTSYTLEFNDKDYSFRGLKKDKTSPKLIKIECRDTDLLELTFDRVLDNET
                     AQDTDTYTISGASVRSAELDSTNTKVRLTTSGIESGKSYELKIRNIKNGDGAVTKSIT
                     KRFTGRRDTSPPKLSKLTVLNNVRLLLEFSDSNGLDKSTAQDKNNYSITGGDGDLEVE
                     TAEVKDMDEDGLWESVYLVTESQEAGTKYTLIIEDMMDDSVLGNNITRIIKKEFRGKS
                     KDKSGPTVANDPRAVTNTMVEVEFSDSNALDVESACDIDCYEIDEGLEILDARIKNPD
                     DLYSVGGRTVLLITEEMEKNERYTLRISGIYDEFGNEMKSSSSDKKYRFKGIGEDRTP
                     PYITSVQCIDSRTLELHFDDTLDEGSAENIVNYRVDGLAIVTKAVLQEDGKTVRLTVS
                     SLSSDRNHTVMMNNIKDLSGNRLSNVSVDILYNGSVYDDDPPEVDYIEAVNRKEVWIH
                     FEEEVYAENAGMEASGIDFKQAGSVLDDGTVVVMVIDGNESLKDDREYEVTKLTGIWD
                     MRGNKYEFEYGLEFDGTDDENDPPEVDDWDQMDVRRFRVVFTEPVLLKGDGVSGIKNP
                     GGVSIEWKAVLNPDDGDDDEAYCTVDYIAKNKNIPPDKEFRFNFTEMVEDYAGLGAYD
                     GIIILESYEEDDDEPYIEDVEAINCKKVQVEFSEAIREPGSYKITYEDDDGKLRTIDI
                     DFVEVDPEDNTRVNIYTEDKMSDEYYYILEPKSAAADIAGNKLDIDDLEIDFEGSSIM
                     SSDYIQGVDLLNEYNLRVYKSSRISRVSSLYRLDGDGNAVGENLIDSTSRISSNVFRV
                     TSKKPLYKNVKYRITADGISYSFRGIADDDNKPEPPLKSSSKEITSFGFNNIAGGIDG
                     NIDKDAKRISLTVPHGTDVKKLAASFKCSEHAVVEVKGKRQISGETTNDFSGEVTYTV
                     IAQDGSSQDYKVVVAIAKELDKSIKAFRFEKPKSAIGEIDEEKRIITVTVPYRTDLSK
                     LVPVIDIPDNTRISVKTGKEPDFSEPVEYIVTAEDGTTKEYTVIVKKAQNTENRITVF
                     AFRALEPVIRAEVGKEQKTIELTVPYGTDRTKLVASFKASPEAVVKVKGEEQVSGETV
                     QDFTNPVKYIVTAQDGTAREYTVIVNAAPSSEKLIKEFGFSVPEAEGVINEAESKINV
                     TVPFGTSLNALAAAFVSSENSKVLIGSDEQKSGETVKDFTNPVIYTVAAQDGSTRNYT
                     VTVRAADENEKILSKFEFAGLNPKARGIINQQNRIIRVSVPSDTFVSNLIASFSYLGK
                     SVYVGEVQQYSGITPNDFSRKVVYRITADDGSYIEYKVIVTVMEGEIKGF"
     regulatory      16932..17033
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            17094..17609
                     /locus_tag="PKU88_09315"
     CDS             17094..17609
                     /locus_tag="PKU88_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HPX69512.1"
                     /translation="MSKAKTIAFVGLLVSMEIIFTRFLSIQTPIIRIGFGFIPIAFTA
                     ILFGPLIGGTAAAIADITGMMIFPKFPFFPGFTLSAFLTGAIYGLFLYRKPITIVNIG
                     KSVLLITILVDLGLTSLWVYMTTGNAASVFLMPRILKSAIMLPIQIVTINVLWKYIGS
                     HIDKARYAKEH"
     gene            18555..19316
                     /locus_tag="PKU88_09320"
     CDS             18555..19316
                     /locus_tag="PKU88_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX69513.1"
                     /translation="MPCQHLEHCPAPAEQNRYIVQEGDTLYSISRFYNISLDDLIEAN
                     PNLEPDRLLPGLEICIPLAAQPADCPFGATTYTVQRNDTFYSIAKKFKMRLSELLKSN
                     PDLNPDALLIGQIICLPKISSSYSNEAYRVRFSYPYLWSRFDSKRHEGIDGFFQISAI
                     SDDAAPEEICKKEAYHKLKPYGTHPTISRTELRGRQAFFIIPSSDQPKEMRGQSAMIV
                     ECSEPVEIEGNNCRYFILRTDKEHLHDIADTLEFF"
     gene            complement(19313..21352)
                     /locus_tag="PKU88_09325"
     CDS             complement(19313..21352)
                     /locus_tag="PKU88_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69514.1"
                     /translation="MKKRAISYFLFLLMTAFVLNACLPAFAVENEDTYDDAPLLDKQT
                     AERELLFFEDNSYNFKDIVKHWARDEMLELSYMDILKGYSSTTMLPEKSISREEYIAM
                     LVRAIGIPTDDEFVESYTDVTDKHWSHKYVTAAKEHGLLSVFSGYYFYPYREITREEM
                     AVITAKAVQNADINGEKLSFKDIPQYYRHKESIDLVTALGIIKGLPDGSFGPKGKATR
                     AQAAAVIGRVLRTREASGEAEDEYLKAFAADYERTVLESLSEGDLGFDTPISLSAGKE
                     ARLNAKRSEQLKIQYPKGFTRSKHMVNESFAVLSKSRYLAEVEAAYELVLENGDRYSV
                     RKSIFLKNIGDKWAVYNSIPEFGADFASGTKINLTWHYIWKDTPDMSSVKKIEGLNVV
                     SPTWFTLANEKGDLEDRGSLSYSNWAHKNGYKVWALVDNQFNSDLTSKLLGNASARAK
                     FISSLISCAKKYKLDGLNIDFENMYIKDKNAFTQFVKELSIQAKADNLVLSVDVSIIA
                     VNSNWSESFDRAALSGYVDYVALMTYDQHWGGSPISGSVAQLSWVERSLKRVLEEVPN
                     EKLLLGVPFYTRLWKEEYVSGSSKPVITSKAISMEEAEKTVAENKAAKIWDPVSGQYY
                     AEYKKGNATYKIWLEDEKSIKLRAELVNKYRLAGIASWKYGLEKPVIWDVIAKTI"
BASE COUNT         5776 a         4950 c         4350 g         6430 t
ORIGIN      
        1 ctgtcaataa gttttatata ttttatattg actcattaca aaacttctag tataattgag
       61 acggggtgaa aaaccatgaa cggatatgag cgcagaacag aattgaaaaa agacaaaata
      121 cgtgctgccg cattagagct cttttgcatg tacggtacgg ataagaccag tataaatgaa
      181 atcgcacaga aggcgggggt tgcaccggcc agtatttata attattttgg gagcaaggaa
      241 ggccttatga aggatacaac cataaacctc cttgaaaacg gctggagagc aagaaaagag
      301 ctgtgggaaa ccgacctgcc ttttcctgag ctggtgaagc gtgccatatc aatgaattat
      361 gattttattg accatatcaa tctggatact ctaagtgcat tgtttaatac cgatcctgag
      421 ataaagaagc tggcagatga ttattataag cacaagtatc cttatatcgt aagccaattc
      481 attgaaaaag gccgaaggga aggctatata cgcaaagata tttcagtaga agcagctaca
      541 atttatctta aaatgtatca ggacctgttc cagcagcctg aaatactgcg gaacagaaac
      601 aaagatttgt ttaaggaatt atgtgattta atgctgtacg gtttggcggg gcagcctatt
      661 acctccaaaa cggagtaagg aaaaaaacat aaaagcctct caattattaa tcaggaaata
      721 cagcttcagg acaaggtatc ttcaccttgt cctattattt tccagttttc agacaaggca
      781 tatgaggcaa gatattaaca tacaatgtta gtgacattcc tgggtatggg agatgattga
      841 agctgtgaaa aaatacttga tgctggggct tctttcgctg ctgattatgg tttctgatca
      901 gccgaagcaa tcggaagaaa gcataaaaat tgtacttggc ggagatgtac ttctcgacgg
      961 gtctgtgggc tggcttattg aaaatcaggg atgcagcagc atatggggag atatgattag
     1021 tatcctcact tccgctgatt tttcaatggt gaatcttgaa aatcccttaa gcagcagggg
     1081 cagcatggaa aaagacaagc agttcacatt caggggaaag cccgcatatg taaaagcttt
     1141 ggaatctgcg ggaattgata tggtttcact ggctaacaac catgtcctgg attacggaaa
     1201 aactgctctc ctggatacaa tgaagcatct ggatgatgcc ggaatcctct atgccggtgc
     1261 gggacaaaat gaggatgccg caagcatgcc ggtatttatt gataagggag cagtaagtat
     1321 ggctgttctg tgctccagcc atgttattcc ctttgtgcac tggcatgcag gcaaaaacaa
     1381 gcccggggtt gcaagcgcat atgatcctgc aaggcttata tcagaggttg catcggcttc
     1441 tgaaaaggca gatatagtcc tggtatacct tcactgggga aaagaaatga aatcccagcc
     1501 tgtccaatac cagaaaaaca ttgcccggat gctgatagat gccggcgcgg atctggtaat
     1561 tggttcacac ccccatatta ttcaaggcct tgaattttat aagggaaaac taatcgccta
     1621 cagcctgggt aatcttgtct tcaccaacag cttcaaagaa acaatgcttc tcagtatcag
     1681 gctttcaaag caggaaatgt tggaagctca tgtgattcca tgcatgatta aatcctcgaa
     1741 aacaatgctc gttaaagatg aacagaaaaa gcaggatttt attgataagc tgaattcact
     1801 gtccccgggt gtacaggtgc ataaggacgg aagaataacc ccggactgat ttaatctcct
     1861 ttaattattt tccgtttaat aatcttgtaa aactattctt ctgaattgaa taacctttac
     1921 attatgatat aatatctatg tattcaacaa atatcttgtt tataatatgc aatggagaaa
     1981 actaacatgg aatatgtaaa tccgttaggt aaagaaaaag tttcaaagct attggccgaa
     2041 tattcggtac ctgccattgt aggcatgata gttaattcca tatataatat cgtcgataga
     2101 atttttatag gaaacagcag tgatttagga accttcggct tagccggaat cactatcggt
     2161 ttcccgatta tgataatact cctctcaatg gggcttctgt tcggggtagg aggagccacc
     2221 ttattctcaa taaaattggg agaaggacag tccgaagaag ctgaccgtgc cctgggcaat
     2281 gctttttcct taatggttat tgccggagca ttattcatga ttacaggtca gattttactt
     2341 cggcctttat taaccttgtt tggtgccagt gaagctgtat taccttactc aatggaatac
     2401 atgagggtaa ttttctttgg ggcgattttt caggtaatag gtatgggcat gaataatttt
     2461 atacgcgctg acggaagccc ccggacagcg atgttaacaa tgttccttgg cgcgggtctt
     2521 aacactttgc tggatccatt attcatttat gtctttaaaa tgggtatgac cggagctgca
     2581 cttgccacca tactggctca atgtatttca gctacttggg tagttatgta ttttatcggc
     2641 aaacacagta aacataagct taaactcaaa tacatgaagc ttaagctaaa tgttgtatta
     2701 acaattactt ctttaggcat tcccggattt gcattacaac tggccaatag ccttctgaat
     2761 gcaatactta acaaaagcct gctcatatac ggaggagata ttgcagtgtc cggcatggga
     2821 ataataaaca gtcttcagac tatattttac atgcccatta ttggcataaa acaaggcgtt
     2881 cagccaatta tcagcttcaa ctacggggca agaaaatttg acagggtaaa agaagcggtg
     2941 aagcttgctg taatttccgc aactgcaatt gtggttttcg gctatgttat aacaaggttg
     3001 ttcccgagac aaatcatatc catattcaat cgtgatcctg atctgataga gtttggaagc
     3061 tacggtataa cagcctggtt cttatgtatg cctgtcgtcg gattccagat aattacttca
     3121 catttctttc aggctatcgg aaaatcaaag gctgcaatgt tcttaacgct tacaaggcag
     3181 gtaatctttc tgattcccgc ccttataata ttccctaaat tctggggcat tagcggttta
     3241 ttgcatgctg ctccctttgc agactttttt tcagctttat tgaccggcat atggttttat
     3301 tatggtaata aaaatttaga aaaatatcca ttagcaacaa tctcaaataa ctcgattaaa
     3361 tgatcattcc gaacacatca tcagaacttt tcaaaaatac tcgcatcaat acatggtaaa
     3421 ttcatggtaa attccatttt tacttgcctt tcttgcctat cttgcgccct cttgtcccct
     3481 gaatagaact gacaaatttc cggcagaata ataaataaga tcaattttaa ggatggtggc
     3541 atatatgaga aatctatcag acgctgaagt actatctctg acagcactgt tcaaaatgga
     3601 acaggacagc ctgacagtat ccagggcttt gcatgcttta atcagcaatg atgaattaaa
     3661 aaagcaaagt gaagcaggca tgctggcggc agaaggaagg gtcaagggca ttcggcagtt
     3721 tattgtggaa aatggcataa ctaatataca ggaggtacag taatatggct tcaatgattg
     3781 gaaatattgt aaagaacaat acggacataa ctgatgaagt tattctgact agtatgctcg
     3841 gctctttaaa agcaattgcc gctgcatatt ttactgcaac aatgacaagt accacaccgg
     3901 agctgaagtc aatgttttca agcgctctgg tacaagtaat gaatagccat tccgtactga
     3961 cagagctggc tgtaaaacat ggttgggaaa agccctacgt ttcacctgcg gaacagctgg
     4021 cagacgtata taaatattca ataacagaaa agcaataacc cttgaggata cggggacgtt
     4081 tcgggacacg gggacgtttc tcctgtccca ctttcagtgg gacaggagaa acgtccccgt
     4141 gtcccgaaac gtccccgtgt cctctttttt aagggctctt ctcctcccac gttctgaaaa
     4201 tcataaaaga ttcattatca cactcgcagc acttccgctg aatcccttct ccgtatttta
     4261 ttatacctcc tacccactcc agcctgttcc tgctgtattt ctcaagaata ttatccgact
     4321 tgcattgaca gcagactacc ttgaattcac ccaagttcat ttacctcctt taatctgcga
     4381 agcaattttt ttctcagaag ctgccagcct aagaccggtc gaaagcattt ttatgacgtc
     4441 aatattcgtt ctgtatacta acgacctgag ccccggagcc ttcataattg tcatcatcct
     4501 gtcaaaatcc ttgttgtcgg cggcgtttaa catcggtctc agggcgctga gttcccggct
     4561 ctttttgtga atatccttca acagcaggtt aacgtcaata cctcttgcaa tacttctggc
     4621 tatcattacg ccggatacta cggcattgaa ctgtccaaag ccaaggaacg gctcgattcc
     4681 gccccctgca acacctgcaa aatagatctt ccccaccctg ttggttgtaa cacttcctgc
     4741 acggtgaggc aatttccatg tttccagcat atcatatttt tttaatatat cccgggactg
     4801 aagaaaacgg tgccaatagc cattcaattc ctcctgtccg atactttgaa ccacatgagc
     4861 tataaccccc ttgtttttac tgtagggagc cgagtatatg tatacccctt ttgtcagctc
     4921 attatccagc cacatctgca gggtatcctc ctcaaaatca ccctcaaata tccctcccat
     4981 tacccatgtc ctcataacct cctgccaaat gccgtaccgg gcaggtactg accaatgtcc
     5041 gtccgccact acgatataat caaattcgtt ttccagatct tccggctgca caaaactgtt
     5101 gaaaataatc tgtgacttaa cttgtgaacg gagctggttt tttactgagt tctcttcttt
     5161 cccgcgaatc agaaaatgac ccaaagggcc ggtaacggct gcctgattgt tgggcgagta
     5221 gtgaatgact tttctgaatt ggctcaaagg cttcagtttg attcccagta ccttgtccaa
     5281 atacttaacc ggatctgagg ccggacgggt tataagcccc aaaagggccg atacatgagg
     5341 atgatactcc ccaatgaact catttcgctc aaaaataaca ggatatattc cgttcctctc
     5401 caattcaatt gcacaggaca atcccgaggt tcctgcccct attatagcaa ctttcacata
     5461 cagcaccctc atgcgctaat acttacagtg ttattatgca taaaagcaga actatcattc
     5521 atcgaaagaa attttgcctc gtttttcttc attttctttt aatgtcattg tttacataat
     5581 taatgcattc gaggttttta ttacaagtgt attacatcct ctcagatgct ataattacta
     5641 tgtcataaaa ttataaagtc ttcatttgaa ggagctgatt cggaatgaaa aaaatcagcg
     5701 gtaaaaaatc aagactttat attccggtat ctataaaatt ttggcttagc catttattcg
     5761 ccatactatg gacaagcctt tcaatttata tatctcttcc ctggcttgaa gacttctcgg
     5821 aggtttttac cttccctgcg gcatttatta tcatttcggg aatagcatac tttccgggct
     5881 acctcaatgc cttcctgttc gcaagcctga tgcttgaccg gcagccgccc tttaaaaatg
     5941 aataccctgc aagggatatt actgttctta ttgctgcaag gaatgaaggc gagcgaatag
     6001 cggacacaat ttattatata gcaaagcagg attataccgg aaatattaag gtcttgataa
     6061 tcgataacgg ttcggaggac gataccgctg ttaatgccct taaggcaggc gaagcacata
     6121 atctggatct atcagttatc agggaagaaa ttcccggaaa attcaatgcc ctgaatgccg
     6181 ggcttgagca tacctcaacc gatttaataa ttacattgga tgcagacact cttctgcata
     6241 ggtctgccat aagatacctg gcagcaagaa tggaaagtgc cccaaaggag gtctgcgcgg
     6301 ttgccggatc agtactggcc aggaacagca ggaatgctct tcttgcaaag atccaggaat
     6361 gggactattt tcttggtatt gcatcaatca aaagactgca gggactatat cagggaactc
     6421 tggttgctca aggtgcctac agcatttata aagctgaaag catcagggag gtagggggat
     6481 ggcctgatgc aataggtgag gatatcgtac tgacatggaa tctgctaaaa aaaagcgggc
     6541 ccatatactt tgaacctctg gcagtggcgt ttaccgatgt gccgacgaca attcgctcct
     6601 tttcaaagca gcgctccaga tgggcaaggg gaatgattga ggctctaaag caggtgaaac
     6661 cttggcagca gccgctgcct tacaccaaat acctgactgg tatgaatctt attatgcctt
     6721 tcctggactt ttgctacacg ttcttctggc tgccgggatt gatactggct ttttttgggt
     6781 attactggat cgtaggcccc atgacattgc tggtaattcc gcttaccctc atgaattatt
     6841 caatattata cgcttatcag ttatacgtat tcagacgctt gaacctaagg gtaagaaaaa
     6901 acatgcgggg tttcatatta ttcataattg tttatcagat gataatgtct cctgtttctg
     6961 tatggggata ccttcaggaa ttcctgaagc tgaagagaaa ctggaaatag caggatttcc
     7021 cgggctgctg caaaaccaca ttttgcagca gctacatatt tttttacagg tttgtacaaa
     7081 ataacagaaa tgaaaattct tgatgccggg gagtggttcc atgcctgcaa tcaatgaaga
     7141 agacagcctc tacaataaaa ctgccgatgt atcaagtgat aatatccgct ctatccttgg
     7201 aagcaatgca gattttgtat cccgtgtcat tcttgtcagg aataaacctg aaataccctc
     7261 tgttcttgta tacatggacg ggctggtaga catcagtctg ataaatgata atatactgaa
     7321 gcccctttcc caggaggagc agtttgattc ctgcagcggg gaagaagatg ccgcaaggct
     7381 tataagcatg ggaaagatat attcctcctc aatgaaggta aggacaaata tgaaagacct
     7441 gactgacgat atactgacag gaagcgcggc attgatattc aatgatataa ggacagcctt
     7501 taccttcgag gccaaagctt ctctaagccg aaccatctca gagcctacag gggaaaccgt
     7561 tattaagggc gcaaaggatt cttttattga ggacttaagg accaataccg ctctgtgccg
     7621 caaaaagctt acctctccat acctgactat tgaacagatt cgtttgggaa aacagacaaa
     7681 aagcaatatt gcgatattat atgtgaaaaa tatagcaaaa agcaaattga tagatgaagt
     7741 gaaacagagg cttgaatcca ttgatattga cagggtgctt gacgcaggtg ttattgaggg
     7801 ggcaatcgtt gatgacaagt attccgcatt ccctcagatt caatataccg aaaggattga
     7861 cagattatgc gcaagccttg ttgagggccg tgcaggaata ctgattgaag gcctgcctac
     7921 ggcttatata gttccgggca cactgctgca atttttgcag gctccggatg actactcctt
     7981 tcagttcatt attgcatcca ttgtccgctt tatcaggttt atgagcatgt ttataacact
     8041 ggtgcttccc ggcttctata tatccattac ctcctttcac ccggaaatgc tgcctgcgga
     8101 actggccttc tcaatagttg catccagggt gggggtgccc ttcccgatgt ttgtggaagt
     8161 actgatactg ctgctggctt ttgagctgtt gattgaagcc ggcgtaaggc tgccaaatgt
     8221 cataggccag acagtgtcta ttgtgggcgg tcttgtggta ggccaggctg ccgttgaagc
     8281 caaactggta tccccggcta ccgttatgat tatagcaatt actgccattg caggcttcct
     8341 tatgcccaac caggatttca gcaatgccat aaggctatgg cgctttatac tggctatatt
     8401 cggcagcata accggtatat ttgggctcac ctttgggttg atattccttc tctaccattg
     8461 gtgcaggatt gagaccttcg gtattccgta cctggacccc tttgttgcca atgaggatga
     8521 acagcttcag gacaccattt tccggcttcc cctcttcacc atgaaaaagc ggcccagcag
     8581 ccttgatcct aaaaacaaga aaaggatgga gtgagctata tatgcggaaa tatgcaattt
     8641 tgacagtcct gctggctttg acggcggctt tatccggctg tatgcaaatc ataatgggaa
     8701 gatcggaaat tgacaagctg tttataatac ggatgttctc tttcgatgaa gcagaagagg
     8761 gcaagatcaa agtaaccgct actacaaagg atctgtcgac gggagaaggc ggaggagagg
     8821 tgcttcagaa aggggaaagt attgcagccg aaggagaaac tgtatttgat gctgtacgaa
     8881 atatgtcaac atacgcagac agaaagctga atgcaggaca tacggaattc atactcttcg
     8941 gagagtccct tgccaggaaa ggcatactcc cctatcttga tttaataagc agacacccgg
     9001 aattccgtta taatgcaaaa ctttatgtag ttaaaggagg taccgcaaac tccctggccg
     9061 aaaaggcaag cacctcgaaa tactttgtag gggacaggtt agcccatata gaagaaaata
     9121 tcggcagaac atccatatcc ggtacggtca cgcttaatga agtactgctt atatttgatg
     9181 aaagatatct ggataccttc atcccatata ttgagccggt tcctacaaag gcaaccggtg
     9241 aaaaaccgga tacctgcgat atgttattga acggttatgc aattttccgg gaggataagc
     9301 ttctctactt cgcttcacgg gaagaatccc gggggataaa ctggattatg aaccgcatcg
     9361 gttcagggat aattgtgtta aaggaccaaa cggggcaaga aatctccatg ataatagtca
     9421 aaggagcttc aagagtaata cctgagattg tagggaatga actccgctgc agggtggaaa
     9481 tctctttctc aaccaatata ggtgagataa tgggcggaag aagcgtattt gacaaggctt
     9541 ccctggatta cattactgag cagcaggaaa attctataaa gcgagaggtg gaaaatgcca
     9601 taaggatagc acaggataag aattccgacc acttcagcat tatttcaaaa ttcagaatca
     9661 aatatcctct gctccgggac tattttcagc aaaactggca ggagctgttt ccagatatca
     9721 aatttgaagt cagtgttgat tccctaatag acggaaccta catgataaat gagccggtta
     9781 ctgccagtaa ggaggctgtg ggtgaatgaa aatatttctg atggtggtaa tagttgtaat
     9841 tgccataaca gcagagatga taccggttat aaaaaagaag cagcacaagg aggcagcagt
     9901 cctcattatg ctgggggctg tgactctggc ctacggctat tattacatta cccatgtgca
     9961 cacagccagc ctggcaaatc ttatgtttga catattccgc ttaaggtaag gagattcttc
    10021 tatgaaaaca ggcaaagata aaatatcggt taagcagctt ttctttgtgt ttacaataat
    10081 ggtgtcttct cccacaacaa gactgcttcc aaaatatgcg gcggcaaaag cggagcaggc
    10141 aggctgggtg tccccgatta taagcacggt gccctttatt ctgctgatac tggctattga
    10201 ttctctattg aagaaatata aagggcagag tatgtctgat attatcagca atattctggg
    10261 gagattcctt ggaaagctgg tactggtggt atacctgctg tgggctctct ggcttgcggc
    10321 catgtataca cgttattatt cggaacgtct gaccagttcg atttatccca atatcagcgt
    10381 tgggttgttc attattttag cccttatacc catagcctac atactgcgtt ccggcttcac
    10441 ccccattgcg aggatgagtg aaatactgct gcccttcata ggggcaatgc ttatcatgct
    10501 ggcgctcttc ttatttccca aggttagagc agataatctg ctgcctgtct atttcaacga
    10561 cataattcct atattcaggg gaagtatcgc agtaacaggg atactgtcct atctgttcct
    10621 tatgttcttc ctgagcgaca atgttgtaaa tctgaaaagc ctcagagtgt tcggctatat
    10681 tgctgcttat gtgaatattt caagcataat gctggtaaat ttcattgtaa taggcgtaat
    10741 gagcagttcc cttgccagga gggtttccgt gcctgtcctt actgttgtaa aacaaattac
    10801 cataatggat attatagaaa atattgaggc tgtagtcgtt gccatatgga ttttcgccga
    10861 cttcacaatg attacaacac ttcttgtaat tgtgctcaac cttattaagt caatatttaa
    10921 cttgtctgat accaggcttc tgattaatat gcttgctgta ctggtatatt ttctgtctat
    10981 gggagtatgt gccagaaggt ttgagcttga ggatttcacc aatagcctgc tcatgcccat
    11041 aaatattaca ctgggctatg tattcccctt tgtactattc gctctatcca agctcaaaaa
    11101 aacaactaac ccttcccgat aaaacggaag ggttagttgt ttacagatca tatcaggttt
    11161 tactgcggat tgtacaagcc catgctctcc atatacttga agacactttc tccatgctgc
    11221 tgctcttctt tctgtatatg gttcagagtc tggcggatgt ttggatccct gaattcaaat
    11281 atagccgtat cgtatgcccc tgaaacatat ttctcagtca tgagaatatc gttgcacagg
    11341 tccccgtcac ttttgcttcc ggtccccgtc cttatgggct gctgtccctg tgccccctgc
    11401 cggccttgct gctgattctg gttcatgccc ggcatctgtc cgcttaacag ctggttgatg
    11461 gtattaagat gctgctgctc gtgttttgca tgagagctga aaagctgctt caactgaggg
    11521 tcttgagcct tgccggcata ttcattgtat ttcttaatgc atatctcctc atgggttttc
    11581 tggtcctgaa gcagcccctt ttccttttga gtcagattgt tcataaaatc acctccggct
    11641 ttagtatttt tcattgtaag caaaatatac taaaagcctt tgatttcacc ctccataact
    11701 gttacaatta ctttatattc aatataggag ccgtcatctg ccgttattct gtaaacaacc
    11761 ttgcggctga aatcattagg tgttataccg ctgtattgct gcacttcccc gacatatacc
    11821 gactttccca gataagagaa tgaagcaatc aagtttgaca caaaagtatc agatggaaca
    11881 gacactctga ttatacggtt ctgctgattg ataattcctc ttgcctttgg attcaagcct
    11941 gcaaattcaa attttgacaa tattttttca ttttcatctg cagctctgac tgttacggta
    12001 tagttacggg ttgagccgtc ctgtgccgca acagtatata tcaccggatt ggtaaaatcc
    12061 tttactgtct ctccgctttt ctgctcatcg cttccaatca ggactttgct gttttccgaa
    12121 ctgacaaatg ctgccgccag tgcattcagt gaagttccaa agggtacggt aacgtttatc
    12181 ttgctctcag cctcatttat aaccccttcc gcttcgggaa cagagaatcc gaattcctta
    12241 ataagctttt cactgcttgg agcagcattg actatcacag tatattccct ggcagtaccg
    12301 tcctgtgccg ttacaatgta ctttacagga tttgtaaagt cttgcactgt ctctccgcta
    12361 acctgctcct cccctttcac ctttacaaca gcttccggtg aagccttgaa ggatgcaaca
    12421 agcttggttc tgtctgtacc gtaaggtact gtcagttcta tagtcttttg ctccttgcct
    12481 acttctgccc tgataacagg ttccaatgct ctgaatgcga aaacggttat ccggttttca
    12541 gtgttttgag cttttttcac tattacggta tactcctttg ttgttccatc ctcagccgtt
    12601 actatatatt ccaccggttc actgaaatcc ggctctttac ccgtcttgac agatatcctg
    12661 gtattgtccg gtatatctat aacaggcacc agcttgctca ggtctgttct gtacggcact
    12721 gtaacagtta taatcctttt ttcctcatct atctccccga tggcggactt aggcttttca
    12781 aacctgaagg cttttatgga tttgtcaagc tcctttgcaa tggccaccac taccttataa
    12841 tcctgtgaag atccgtcctg ggcaataacg gtataggtaa cttccccgga aaagtcattg
    12901 gtggtttccc cgctgatctg cctttttcct ttcacctcta ccacagcatg ctccgagcac
    12961 ttgaagcttg cagccagctt cttcacatcc gttccatggg gcactgtcag gctgattctc
    13021 tttgcatcct tatcaatatt tccgtctatt ccgcctgcaa tgttattgaa gccgaagctt
    13081 gtaatctcct tggaagagga ttttaggggc ggttccggct tattgtcatc atcggctatg
    13141 ccgcgaaagc tatatgatat cccgtcagct gttattctgt atttaacatt cttatacagc
    13201 ggtttttttg atgtaactct gaatacgtta ctgctgatcc tcgaagtact gtcaatcagg
    13261 ttttctccta cggcattacc gtctccgtcc agcctgtaca ggctgcttac cctggatatc
    13321 ctgctgcttt tgtaaaccct gagattgtat tcattcagca aatccacacc ctggatataa
    13381 tcactgctca ttatactgga gccttcaaaa tctatctcca gatcatcgat atccagcttg
    13441 ttccctgcaa tatccgccgc agccgactta ggctccagga tataatagta ttcgtcactc
    13501 atcttatcct cagtatatat atttaccctg gtattatctt caggatcaac ttccacgaag
    13561 tcaatatcga tagtcctcag cttgccgtcg tcatcctcat aagttatctt atagctcccc
    13621 ggctccctta tggcttcgct gaattccacc tgaacctttt tgcagttaat ggcttcaaca
    13681 tcttcgatat acggctcatc atcatcctcc tcatatgatt caagaatgat gatgccgtca
    13741 tatgcgccta accccgcata gtcttctacc atttcagtaa aattgaatct gaattctttg
    13801 tcagggggta tatttttatt cttagctata tagtccacag tgcaataagc ttcatcatca
    13861 tccccatcat cgggattaag cactgctttc cattctatac ttacccctcc gggatttttg
    13921 attcctgata ctccatctcc tttaagcagt accggctccg tgaaaactac ccggaatctt
    13981 ctcacatcca tctgatccca gtcatctact tcaggagggt cattctcatc atcagtacca
    14041 tcgaattcca ggccgtactc aaactcatat ttattgcctc tcatgtccca tatgcctgta
    14101 agtttggtta cctcatactc tctatcatct ttcagcgatt catttccatc tataaccatc
    14161 acaaccacag tcccgtcatc cagtacactt cccgcctgtt tgaaatcaat gcctgacgct
    14221 tccatcccag cgttttccgc atacacttcc tcttcaaaat gtatccagac ctctttcctg
    14281 tttacagcct ctatataatc cacttccgga ggatcatcgt catacacgct gccgttataa
    14341 agaatatcta cgcttacatt tgaaagcctg tttcctgata aatccttgat attattcatc
    14401 atcacggtat gatttctgtc tgaagacagg gaactgaccg taagcctgac cgtttttccg
    14461 tcctcctgca gtactgcctt tgtaacgata gcaagcccgt ctaccctgta gttgactatg
    14521 ttctccgccg atccctcatc caaggtatcg tcgaagtgca actccagagt tctgctgtct
    14581 atacactgaa cagaggttat atatggtgga gttctgtcct cgcctattcc tttgaacctg
    14641 tactttttat cgctgcttga agacttcatt tcatttccga attcatcata aatgccgctg
    14701 attctaagtg tatacctttc atttttttcc atctcttccg taatcagcag cactgtcctg
    14761 ccccccactg aatacaaatc atcgggattc tttattcttg catccaggat ctccaggcct
    14821 tcatctatct catagcagtc aatgtcgcag gctgattcca catcaagggc attggaatcc
    14881 gaaaactcga cttctaccat tgtgtttgtg actgcccttg gatcattggc aactgttggc
    14941 cccgatttat cctttgactt ccctctgaat tcctttttta ttatcctggt tatgttgttt
    15001 cccaataccg agtcatccat catatcttca attatcagag tatatttagt gcctgcttcc
    15061 tggctttcag tcacaagata cacggattcc cacaaaccgt cctcatccat gtctttgacc
    15121 tcagccgttt ccacctccag gtccccgtcg ccgccggtta tactatagtt gttcttgtcc
    15181 tgagccgtgg atttatccaa tccgttgctg tccgaaaact caagaagcag ccttacattg
    15241 ttcagaaccg taagcttact cagctttgga gggcttgtat cccttctccc tgtaaatctt
    15301 tttgtgattg actttgttac cgctccgtcg ccattcttta tattgcgtat tttaagctcg
    15361 taggattttc ctgactcaat gccgctggta gtaagcctta ccttggtatt tgtgctgtcc
    15421 agctccgccg accttacgct tgctcctgaa atcgtataag tatccgtatc ctgggctgtt
    15481 tcattatcca atactctgtc aaaggtaagc tccagcaggt ctgtatccct gcattcgatc
    15541 ttaatgagtt ttggagaagt cttatccttt ttcaggcccc tgaagctata gtccttatca
    15601 ttgaattcca atgtgtagga agtgttttcc ttcattgccg aggtatctat tattacagca
    15661 tattcattct tcatacttac tttgtttatg tcaagatgct taatttcata cagatcctca
    15721 tcttccgcat cggtaccgtc aatggactcc ttaaaaacaa cctccacctt gctgtttgaa
    15781 atagccctta cgtcttttac ctctgcctcc aattccttgg agtacaagcc tgctgatatt
    15841 gcatcccact tatccacatc ccctatatcc accaggtatt ctataagagt cctgcgctct
    15901 cccttcattt tggtattaag agcctcaacc gtagcaatcg caaggctttt tactgtaaag
    15961 ttcctgatac cggctacctt gtacagcccc ttttcttctg caaaatcaag cacttcatcc
    16021 cagtcaaagt ctcccttacc gtcaattttc tgcttatagc ccaggctttc caggagtacc
    16081 ttgtaatatg ccctcgaatc tatcaggctg taaggcatga agttcactcc gtcaccaatc
    16141 cacccaagct ccgggtgatt tttcaggtaa ctcagcacgt ttctgccttc ttcccaggca
    16201 actacgccgg catccttgaa gttttttcct ccgtaatagt ctaatgcatc ctgttccaaa
    16261 cctttaagcc tcagaaacat tattgccgcc tgcagcctgc tggggcgagt ttcaaggtat
    16321 tcttcgtcta caattccggt atgtcccttc agtattccca agtctctgca aatacgcccc
    16381 tcgtagccga tatcatcatc ggcaataacc tgtaccggaa gtattgcgct tagaaccagc
    16441 gcaaaaattg taataacagt tacaaatttt ttgaccatca aacgtacctc cttaaaatat
    16501 cagtgcctac cgataaaaat gtcgatggtg caccccttta atgttaaatg gcagatactc
    16561 gcctaataat tagatatgac tcttttcatg caaactgtac tgctttgatg atttgtaaca
    16621 tactcttaac agccctgtaa tatttggcgg caccatctgt atcctcctta ttttatagta
    16681 gtttccattt tctttcaata attacagttc gtcccatttc tactatattt tcagccgaca
    16741 gtcaattgcc gtataatgct gtaacttttc gcaggctatg agattaaatt tgattgatcc
    16801 ggtaataata tggaacgaaa acaatccggt gcggttgaaa taatacaaga ctggccaggc
    16861 tgtagtatat ggcgggcata cttgcaatta ccgatcattt gatgtacaat ttcaatacaa
    16921 tataaaaata tggtagagta ggcttacatg cgtaaagtgc tggttgtacg ggaagttgac
    16981 accagacgaa aagcccgcca gggcttgcgg tatgtatgct gcattccgct gccacttagg
    17041 agccatccct ctttaagtgg tctatttgcc atttaaagag aggaggtgat tgtatgagta
    17101 aagctaagac aattgctttt gtcggattgc tcgtatcaat ggaaatcata tttacccgtt
    17161 ttctatccat tcagactcca ataataagaa ttggcttcgg gtttatacct attgctttta
    17221 ctgccatact gtttggtccg cttataggtg gaacggctgc tgcaattgct gatatcacag
    17281 gcatgatgat ttttcccaag tttccgtttt tccccggatt cacattgagc gcatttttaa
    17341 ccggcgcaat atatggcctg tttttataca ggaaaccgat tacaatagtc aatataggaa
    17401 agagcgtttt gctgattact attcttgttg atctcggact gacttcactt tgggtatata
    17461 tgactacggg aaacgctgca tctgtattcc tgatgccgag gattctgaag agtgccataa
    17521 tgcttcccat tcaaatagtg acaattaatg tactgtggaa atatatcgga agccatatcg
    17581 acaaagcgcg ctatgcaaaa gaacattaaa aaggcttgga gccggtcggc tccgagcctt
    17641 tttcacataa gaagaataat gcgacgcatc tgtagtttac ataagcctcc gcaagagaca
    17701 atatagtata tcgacgcaca cggattatga ttttgacagg actactgtga agcaaaacaa
    17761 attaatacga atccgcctca cgccggttaa tttttcgctt ttcttcgtta tcctcattga
    17821 ttggcgccag ctaatctgct tcctctgcct cgaaaatcaa caaatttcct cggcgtgagg
    17881 tcaatagatt ttcgagcaag ctgatttgat ttgtggcaag gcgggcgctc ttgtaatgct
    17941 tatcgcctta caagagcgga caacgcagct acaaggtaaa tcagctgctc taaaacggat
    18001 tcgtatcagt ttgttttgct tagcctttgg aaaagtccta acggtgccaa aggccatccg
    18061 tgacccgtgg tacctaagcc ggcgtccttg ccggctttac ggacttttta ccaaagccat
    18121 atcagaggcc tgttatcaaa atgcataaaa gtgtgccctg ctatactata caagctcttg
    18181 cttagtatgt atcctacaga gcatcatatg ttatacaagg ccagtgccct ttaggaatta
    18241 tgctgcatta aggaaatgca aataccgacg tggggaagat atctttgacg aaactttcca
    18301 cctgacctcg gaacgggaat accccaggca aaataaccag acgggctcgc agagatggga
    18361 cccacgccag tgattttgct ggctttggaa agtgaggaga acatacttcc ccaacagtcg
    18421 gagtacttca catactattg taatgatgtc gcactattct tctattgtgc acaagatata
    18481 ttgtaacctt caaatgaata tattatatta cccggcattt ttagggtata taatatttca
    18541 aagaggtgag cgtaatgcct tgtcagcatt tggagcattg cccggccccc gccgagcaaa
    18601 accggtacat agtgcaggaa ggagatactc tatattcaat atcaagattc tacaatattt
    18661 ccctggatga tttgatagaa gccaatccta atctggagcc cgaccgtttg ctgccgggac
    18721 tggaaatatg cataccactg gcggcgcagc ctgcagactg tccctttggg gccacaacct
    18781 acaccgtgca aagaaacgat accttttatt ccattgccaa aaagtttaaa atgcgattgt
    18841 cggaattgct taaatcaaat cccgatctca atcctgatgc gctgctgatc gggcaaataa
    18901 tatgcctgcc taagatatca tcaagctatt ccaatgaggc ctatagggtc aggttcagct
    18961 acccttacct ctggagcagg tttgacagca agcggcatga aggaatagac ggattctttc
    19021 agatatctgc catttccgat gatgcagcac cggaagagat atgtaaaaaa gaagcatacc
    19081 ataagctgaa accttacggt acgcatccga caataagcag aactgagtta cgcggccggc
    19141 aagccttctt tattataccc tcttccgacc agcccaagga gatgcggggc cagtctgcta
    19201 tgatagttga gtgttcggaa ccggtcgaga ttgaaggcaa taattgcagg tacttcattc
    19261 tccggacaga caaggagcat ctgcacgata ttgccgatac attggaattt ttctagattg
    19321 tctttgctat tacatcccat attacgggct tttcaaggcc atatttccat gaagctatac
    19381 ccgcaagcct gtacttgttc actaattcag ccctcagctt tatggatttt tcgtcttcca
    19441 gccatatctt ataggttgca ttgcccttct tgtattccgc atagtattga ccgcttacag
    19501 gatcccaaat ttttgcagct ttgttttctg cgacggtttt ttccgcttcc tccatggaaa
    19561 ttgccttgga ggttataacc ggtttgctgc ttccgctcac gtactcttcc ttccagagcc
    19621 ttgtatagaa gggcaccccc aacagaagct tttcattcgg tacttcctca agtaccctct
    19681 tcaggctccg ttcaacccag gacagctgtg caacagagcc gcttatgggg cttcctcccc
    19741 aatgctggtc ataggtcatt agcgccacat aatcaacata ccccgacagt gcagccctgt
    19801 caaagctttc agaccagttg gagtttacgg caattattga cacgtcaaca gacaacacca
    19861 gattatcggc ttttgcctgt atggaaagct ctttaacaaa ctgtgtaaaa gcattcttat
    19921 cctttatata catattttca aaatcgatat ttaatccgtc aagcttatat ttctttgcac
    19981 agcttataag ggaacttatg aattttgctc ttgcgcttgc atttccaagc agtttgcttg
    20041 tcaaatcgga gttaaactga ttgtctacca aagcccagac cttatacccg tttttatggg
    20101 cccaattgct gtagctcaag cttcccctgt cttccagatc gcctttttcg tttgccagtg
    20161 taaaccatgt tggtgagact acattaaggc cctcaatttt cttgacactt gacatatcgg
    20221 gggtatcttt ccagatataa tgccaggtaa gatttatctt tgttcccgag gcaaaatctg
    20281 cgccgaattc cggaatgctg ttatatactg cccacttgtc tcctatgttc ttcaaaaata
    20341 tgcttttcct gactgaatac ctgtccccgt tctcaaggac cagttcataa gcagcttcaa
    20401 cttcagccag gtacctggat ttgcttaata ccgcaaagct ctcattgacc atatgcttgc
    20461 tgcgggtaaa ccccttggga tattgtattt ttagctgttc agacctcttg gcattaagtc
    20521 tagcttcttt tcccgctgaa agggatatgg gagtatcaaa gcccaaatcg ccttcgctca
    20581 gggattccaa aacagttctt tcataatctg ccgcaaaagc ctttaggtat tcatcttcag
    20641 cctctcccga agcctccctg gttctcagta ctcttcctat taccgccgcg gcttgtgctc
    20701 ttgttgcttt tcctttaggg ccgaagctcc cgtcgggcag ccccttgatt attcccaatg
    20761 ccgttaccag gtctatactc tccttatgcc tgtaatattg cggaatatcc ttgaagctaa
    20821 gcttttctcc gttaatgtca gcattttgta cggcttttgc cgtgattacc gccatttcct
    20881 ccctggttat ttccctgtag ggataaaaat aataaccgct gaaaacagac agcaaaccat
    20941 gctccttggc cgctgttaca tacttatggc tccagtgctt gtccgtaaca tcggtgtagc
    21001 tttcgacaaa ctcatcatca gtaggaattc ctattgctct tacaagcata gctatatact
    21061 cttccctgga tatgctcttt tccggcagca ttgtggtact gctgtaaccc ttcaaaatat
    21121 ccatatagct cagttcaagc atttcgtccc ttgcccagtg tttgactata tccttaaaat
    21181 tataggaatt gtcctcaaaa aaaagcaatt ctctttctgc agtctgcttg tccaaaagag
    21241 gtgcatcatc atatgtatcc tcattttcga cagcaaaagc aggtaaacag gcattcaaca
    21301 caaaagctgt cattaataaa aataaaaaat aacttatggc tcttttcttc ataacttcac
    21361 actccccata atattatatc aattatgtaa actagatttg ttacagggag atttcaaaaa
    21421 acttgtatta aatagaaaaa gagacagggg gagacatagg gacacgggga cgcttcggga
    21481 cacggggacg tttctcctgt cccgct
//
LOCUS       DAOOAB010000043        21038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3317_length_21038_cov_16.242577, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000043 DAOOAB010000000
VERSION     DAOOAB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_21038_cov_16.242577"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1158
                     /locus_tag="PKU88_09330"
     CDS             238..1158
                     /locus_tag="PKU88_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPX69515.1"
                     /translation="MNALKRISQVFEASEQITFDDSSKIILMSDCHRGDGSWADDFSR
                     NQNLYFTALTHYYKNNFTYIEIGDGDELWENNNFFNIKQVHCNVFWLLSRFYSEGRLH
                     MIYGNHDMVKKYNRFVRSNMHRYLDEPARRHMPLFINIKFHEGLILKYRPTGGKIFLI
                     HGHQVDCLNDRLWKLARFLVRYLWRPLNNLGVNDPTRTAKNYKKKETAEKKLTEWVIK
                     EGHVLIAGHTHRPMFPEVNEPPYFNDGSCVHPRCITGIEIAEGYILLVKWSVKTKDDG
                     TLFIGREVLEGPRKLVDFFACKDRTKKLLL"
     gene            1223..2092
                     /locus_tag="PKU88_09335"
     CDS             1223..2092
                     /locus_tag="PKU88_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPX69516.1"
                     /translation="MKRLAYVFVISAAVLWGTIGIYSNQLSQYGANVLQIVSIRAIFA
                     APAIFVFILLKDIKLLNIDIHDWKYFFGTGILSFVFFNWCYFTAIDKTTLSTAAILLY
                     TAPAIVMVFSIILFKEKLTRRKVAALILTFIGCAFVTGVVKGSQDISPLGILAGLGSG
                     FGYALYSVFGRYALKKYDPITVTLYTFVFASIGLIPISDIKGLFGLLSSTVAFYNAVL
                     LGLAATVMPFLLYTKGLSYLETSKASIIAAIEPVVATIIGIILFGEPITVFKIMGVFL
                     VVFAVSILEQKKS"
     gene            complement(2413..3426)
                     /locus_tag="PKU88_09340"
     CDS             complement(2413..3426)
                     /locus_tag="PKU88_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPX69517.1"
                     /translation="MNYDEVLKNAKANLNGTCRVCPVCNGKACSGEVPGMGGKGTSEA
                     FTANVDALKKYKLNMRVLHNCKNPDTSIELFGKKMSIPVFAAPVAGTNFNMGGKFQEQ
                     EYISWIIKGCIQGGIYPMFGDAAIDSLLVTNLEELKKSGGEGIAIIKPWTNDKVIEKI
                     RMAEKAGAYAVGIDIDAAGLITLSLLGKPVGPKSVEDIKEIAESTKLPLILKGIMTPD
                     EAELAVESGAAAIVVSNHGGRVLDQTPGTADVLPEIAKKVKGKITILADGGVRNGVDV
                     LKMLALGADAVLIGRPFVPASFGGQAEGVKAYIDSIAADLKSAMILTGCNSIEDVSSR
                     ILF"
     gene            complement(3530..5167)
                     /locus_tag="PKU88_09345"
     CDS             complement(3530..5167)
                     /locus_tag="PKU88_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPX69518.1"
                     /translation="MNSLVLLFIGIAAFLIAYTIYGKWLANQWGIDPTKKTPSCTLRD
                     DIDYCPANAKVLLGHHFSSIAGAGPITGPIAAAVFGWLPVYLWIVVGSIFVGGVHDWG
                     SLFASVRHDGKSIGEIIRANIGENGKKLFNIFAYLTLILVVAAFTDICAGTFAFDPAK
                     PEAITGARAGTASILFIFLAMGFGYFVYRKGASLSVSTVAGVALLFFCIWIGYQFPVL
                     KFSKLTWDVILIIYIFSASTLPVWLLLQPRDYLCSFLLYAMLAGAVIGVLLVRPAMAI
                     PASVGFTVGEGAKAQYLFPMLFVTVACGAVSGFHSLVASGTSSKQVDNEKDTQLIGYG
                     GMLIEGVVAIIALIAVSYVAGATGTPAQIFAAGVADFMSSFGIPVPVGTVFVTLAFSA
                     FALTSLDTATRLGRYMFQEMTTKADGTKSPLNNMYTATAVTVGLSALLLSYGYTKIWP
                     IFGSANQLLAGLALLAVSAWLAKSGKKNTMTIIPMVFMFCVTLTALVLVARTNFAGGN
                     VIIGSLAAILFVLAIVLIITTIKTFSGDKSKNQNVDA"
     gene            complement(5253..5759)
                     /locus_tag="PKU88_09350"
     CDS             complement(5253..5759)
                     /locus_tag="PKU88_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69519.1"
                     /translation="MKYDEYIKLLTSKLEKNFAIERDIVIFDNKIDILAKFSNISVRT
                     FITQHDIIDQYNNHEHIYIKRCDDVNEKDIISFGQFLKRISDECIKPDKDHMSTYVTG
                     VLIGNHINENAIKAVEKYNYKKAFCFYLKGWCDVRLIYVGLDNSEIITNKDGKKVKKL
                     YEFASLNK"
     gene            complement(5962..6663)
                     /locus_tag="PKU88_09355"
     CDS             complement(5962..6663)
                     /locus_tag="PKU88_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPX69520.1"
                     /translation="MNCIIVDDEYPSIQELSYFITNFSSITISETFDDSIKALEYIQR
                     HSVNVIFLDINMPKLDGLTFSKVINTLPVKPVLVFITAYREHALEAFEVSAFDYILKP
                     YSQTRIADTLQRLENSTAVRLHNDKITLWKNEKLYVLDADDIYYCEAHKHEVYVYTKD
                     KRFVVASSISDFHKKLRQDCFFRCHRSYIVNIDKITEIIPWFNNTYMLKLKGLDAEIP
                     VSRQNILLFKKLMGI"
     gene            complement(6703..8373)
                     /locus_tag="PKU88_09360"
     CDS             complement(6703..8373)
                     /locus_tag="PKU88_09360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPX69521.1"
                     /translation="MIDLIKNLINSLIYVIIVAFIVSNLSVFKKIIQKDAFKKSDLLI
                     LSVIFAVFGIISTYTGTDVLGAIANTRIIGVMTGGILCGPFVGIVSGIIAGLHRFLYD
                     IGGITSVPCAISTILAGFLSALIYKKGIRYQKWLYGLAGGIIIESIEMLLILLMSKPF
                     SSALAIVKSIYVPMSFTNAIGICILILLIQNSFKEKEQMAAMQAQLALEIANKTLPYF
                     REINGDSLKKVCTIIRESTGAAAVSITDKTTILAHVGLGSDHHISGDPIQTFATKQVI
                     MNGQMRIMNSPEEIECSSEDCQLKSGLIVPLKEKDEIIGTLKLYFAKRNGISFTHEKL
                     AIGLSQLISTQIEISKVGKLEELAAQAEIKALQAQINPHFLFNALNTIIALLRINPNS
                     ARELIVNLSTYLRYNIEETSIFVDISKELQQVRSYIEVEKARFGDKLNVIYDIDESIH
                     VKIPSLVIQPIVENSIKHGILKGSGTGNIVISVKKHNPDEIRVSVEDDGIGIPQAFIE
                     NLHAGKVKENKIGISNVDNRLRHIYGTGLNIERLDVGTRVSFMLNMSA"
     gene            complement(8517..8972)
                     /locus_tag="PKU88_09365"
     CDS             complement(8517..8972)
                     /locus_tag="PKU88_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPX69522.1"
                     /translation="MTLEEYKKIISDAIKNEEEAYSFYRDVAKKTKDKNISEIFENLA
                     KDELSHKVILQDYFKDTGKKLEFDSTADYKVAESVDKPKLSIDMKPADAIALAMKREE
                     EAMDMYKGFAAISTDKEQKETFLGLAAMEQGHKTKLEDLYVGAAFPEVW"
     gene            complement(9179..9748)
                     /locus_tag="PKU88_09370"
     CDS             complement(9179..9748)
                     /locus_tag="PKU88_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69523.1"
                     /translation="MKGKVIVLVFAVIIALFAGCAPEPAAPETPPQTTPPEATPQEAT
                     PDVVTTASIVADADAFKKAISGNGTWIICTTKDLSINEDLILEGEFHDKNDPAKDLKR
                     KIALYAQDADKNVTARYTLTAPKLTVKSPNARIQGGTFKGDVYVSAKNFELVDGTIDG
                     NVYFTTDDAKSTFKIDDKSKVTGKQELKK"
     gene            10038..12023
                     /locus_tag="PKU88_09375"
     CDS             10038..12023
                     /locus_tag="PKU88_09375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPX69524.1"
                     /translation="MQEAAGAFKLYIAKNNAVMIQAADNQKAVFYFFIILLIAAAVAI
                     IHYYAARNRRGTVNSKKEGVISYRTIIQLMQEGFALFEIIYDQGGKPIDYYFIDSNEA
                     AEVISGIERKTSINKSVFDILPDSAAELAKKLNEVTFAGEPYKDEIYVKEWGKYLSVN
                     MYKAEKKLLAVMFSDITQKKLADEAMQKQKLSYEALFRNSSDAIIRFDQNHNIIDINE
                     KFTELFGYTLDEIYGRELDSVLAPKEKFDEAKVLTKNVLNGNITAMEESVRLGSDGKT
                     REVSIKGVAIVLNDKIIGGYGIYTGISRRKKAERELMYMNYHDLLTGLYNRRFFEEEL
                     KRLDTERNLPISIIMADVNGLKLFNDAFGHAEGDKLLVKAAEIISKELREDEIVARLG
                     GDELVILLPGTDSEEARSIVKRIRASISNIRLKSIELSISFGWDTKNSADQDMNDILK
                     NAEDYMYRNKLFESPNIREKRIKAIINSFYENNSEEEQHSKRVSSLCEATGAAMGMAG
                     AALKELRTLGLLHDIGKIAIDNSILNKPDRLTKEEWNEIKRHPEIGYRILSSVNDMAE
                     LAVFILAHHERWDGKGYPKGIKGTDIPLQARITAVADAYDAMTSKSAYRNPLSESIAV
                     DELEKNAGTQFDPEIVQIFIDKVINKKVKTSVDIDVT"
     gene            12064..12828
                     /locus_tag="PKU88_09380"
     CDS             12064..12828
                     /locus_tag="PKU88_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPX69525.1"
                     /translation="MEYTVQKLGKLAGVSTRTLRYYDEIGILKPARINASGYRIYGQK
                     EVDRLQQILFYRELGVGLDSIREIVTAPSFDGAKALREHRGKLLEKREQLDMLIANVE
                     KTIALTEGRISMTDKEKFEGFKQKLLDDNEKKYGKEVREKYGESAVEKSYDKVKNMTR
                     EEYDELTKLSEEITKILGEAFKTGDPAGDLAQRAADLHKQWLTFYWDSYSKEAHAGLA
                     QMYVDDERFRAYYDKKQPGTAEFLRDAILIYTGMKK"
     gene            13019..>15393
                     /locus_tag="PKU88_09385"
     CDS             13019..>15393
                     /locus_tag="PKU88_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPX69526.1"
                     /translation="MKCISKFIIFLFALLLTLSAFPVSAASEAPEKEELRGVWVATVA
                     NIDYPSKSTADSAILKSEALKILDDAEAMGLNAIFLQVRPASDALYKSKLFPWSKYLT
                     GRQGLAPDNGFDPLEFWAAEAHKRGMELHAWINPYRITKKTPKEPKHDLASLDPSNPA
                     RLKPEWVVKHTDGNLYYNPGIPEVRKLVIDGVLEIIENYGIDGIHLDDYFYPGKDFSD
                     KAAYAKYGTAYGNIDDWRRENVNKLISELSKAVKAADEAVSFGVSPFGIWANKSANTL
                     GSDTRGMQTYYDQYADTRKWVKEGLLDYIAPQLYWYIGYEIADYSRLLSWWADVTAGT
                     GVDLYIGQAAYRTGDTDPKSPWYGIAEMEKQLKLNSENKEIKGSIFFSNRSLCNNPAL
                     CAALQSLYKRKSSIPISVSRPAEDIQTTLEKYYLNGSSDPEKPLYLNGVPVENRSARG
                     YFGVLVPLTEGANSFTLSQEASEVTRVIYRKTADSEPAKMSGAEIISASVFPQSQEYR
                     NPGEKITLSCEAPYGSTVTVQIGGKRYLMKSSGAVTGTSGLYADKFTCEYTIPAYKGT
                     PRNIDLGKPVYTMNYKGTAKSCTAPAKIGVIMKGSPFYAKVEKEVIYTYNEASSSDGA
                     AYELYSGMVDSVTGMSGGYVRLSLGQWVKDSDVAVYTSKAQSRPIIKDTEYISGDGWD
                     SLKLDISAPPAAIADFDGALLKINVSADSQAASPELPDDSPFSSVAVAKKVNNTEYTM
                     ALKKNRSISGYYMQKTKTGLVLNIKRPVKSNNSNEPLSGLTIMLDPGHGGS"
     assembly_gap    15394..15493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15494..16053
                     /locus_tag="PKU88_09390"
     CDS             <15494..16053
                     /locus_tag="PKU88_09390"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPX69527.1"
                     /translation="EPLSGLTIMLDPGHGGSDTGAIGPLGTEYPEKTINLNIAFKLQS
                     ELEQLGARVFLTRAADVNVSLEERLAASRNAKPDMFLSLHSNSMAEDVDISKVDGFAV
                     FYREKLALPLAETIFQNTLTHLNRTNKGLHNKNFYVLRGTWTPSILVESGFVPNPYEF
                     EWLIDENEQAGLAQSIAKSVVEYFK"
     gene            16123..17235
                     /locus_tag="PKU88_09395"
     CDS             16123..17235
                     /locus_tag="PKU88_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69528.1"
                     /translation="MSELRKMKAIVLLSIAIMTAIVILPASAAVGQQPPFSEVKEKLS
                     GISKEEKEILQKLFILAQEIEIAEAEAEELSRETEAISKEIKGLEEDIAKEELAYEKK
                     RKSLEQVLKSYQRMGAGSFLEIILDSDSLSTFLHRLNILRDLTKNTGTLLEQLESSGR
                     KLAEEKSKLSEKLALVKDKESMSRTALADKNRLKAEKEAYLASLKGEKEHYQEYLSNL
                     ERVWNELKPLLSETAKEFSRMIEEEGLPYDALKISFSLFEVKGAIDDGTINKIISENA
                     NLEEMIFAFHQEAAEIRIPDKNLILTGTFAIQEGHTLKFQAREGSFFGLPLEKGVLEE
                     LFGEEGMMLNLEPLLAGNDIHAVRIKEGYIELIHRF"
     gene            17263..17937
                     /locus_tag="PKU88_09400"
     CDS             17263..17937
                     /locus_tag="PKU88_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX69529.1"
                     /translation="MIEAKGVCLEYPDGTKAIKNIELSVKPGELVYITGPSGSGKTSL
                     LKLFMGEEYPTAGSLRVLGQHISRGQASRIRGLRRMIGPVFQDFRLIKGRTALENVML
                     GMRFLDIPSGCIRENAGNALKKVGLEQKMLSLVEHLSWGECQRVAIARAVSREPVLII
                     ADEPTGNLDKDNALNILELLKSFRNDNKAIIITTHATHLIEDERYAVHIRMDNGNMLK
                     ERSGEA"
     gene            17938..18822
                     /locus_tag="PKU88_09405"
     CDS             17938..18822
                     /locus_tag="PKU88_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPX69530.1"
                     /translation="MKNILYNTGYFIKETKTIMRLNLLSSILSFLSIGLIFFILAMMI
                     SGWWVSSHVVLAIQEEAEISIYFSEDMDAAGAERLAEEIENIEGVYEARLVSEEEAYS
                     RMEKILGKEARVLEYLDDNPFSPFIEAKIQIEDIGLIPGKLDLIEGIEHVRDNREVLE
                     RILGISQTIKILGYLIIAAVGISTTVIISHIIRMGIYDCREQINTLRLMGAPEAFIGF
                     PFMLEGLLLTLGGGALSSALAAYALKYLYAQAAGPIAFIPLPPREALVSDLTILIMLL
                     STALGTAGSFFGLSSAKN"
     gene            18904..20091
                     /locus_tag="PKU88_09410"
     CDS             18904..20091
                     /locus_tag="PKU88_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HPX69531.1"
                     /translation="MSFKMTGKVTWVGKIDWELRRFHGDEYSTHRGSSYNSYLIKDKK
                     NVLIDTAWAPFAKEFVENLKKEIDLDKIDYIIANHSEIDHSGALPLLLAEIPNTPVYC
                     TANGAKILKGHYHKDWNFVTVKTGDTLDIGESKLTFVEARMLHWPDSMFVYMSGENIL
                     FSNDAFGQHYASELMYNDMVDNAELYQEAIKYYANILTPFSTFVTKKIEEVLSFNLPV
                     NIIAPSHGIIWRDNPAQIIQKYMDWAKDYQENQIVIVYDTMWNGTRRMAEAIAEGIRS
                     VDKEVAVKLFNAAHSDKNDVITEIFKSKAVLFGSSTVNKSIMHAVGGMLDMVKGLGFK
                     NKKAAAFGSYGWSGESVGILTERLKDAKFEIVSDGIKELWNPDQDAMNRCVQFGADFA
                     GKL"
     gene            20166..>21038
                     /locus_tag="PKU88_09415"
     CDS             20166..>21038
                     /locus_tag="PKU88_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69532.1"
                     /translation="MLIKMFNKKAFFRSASLVMSIVVLLGAGLLTGCTAPAEDNTGGK
                     AEDNNPPAVEESIEDRIIPEFMALAVGNPKPDAIIEFMDKSIADVSGENASKMLDELE
                     KSLKSNLPELEEKYYSTAVQEAMFNAYKSEFDLSKLDSIQDAEVKSLIDETKAMGYKV
                     ETAEGMFFPIINYEFLKKFSSYAEDDMKDYIDIMAAESNKVPAKDGALVIGWDEVIER
                     ALAQENFMAKHSSSAKVGSIKELQKKYIMFILYGLNNTPLFSYDTKMMNPEAKDAYTK
                     VLKEKGDSELLQLLG"
BASE COUNT         6409 a         3961 c         4685 g         5883 t
ORIGIN      
        1 agaatattgg aaagctaagg ctgaataaag aggacacggg ggaggacacg gggacgcggg
       61 gacatgggga cgtttcccct gtcccacttt cagtgggaca ggggaaacgt ccccatgtcc
      121 ccgcgtcccc gtcgcattat tcttcaattg tgcaacagtt cctttgatct agcctcaaat
      181 cacaagtcat gtgaatataa tgtattgtag tttgatgact tgacgaggtg aggcaaagtg
      241 aatgcattaa agcgtatatc ccaggttttt gaagcatcgg agcaaattac ttttgatgat
      301 tcctccaaaa ttattttaat gagtgattgt cacaggggag acggaagctg ggcagatgat
      361 ttttccagga accaaaattt atattttact gccttaactc attactataa aaataatttt
      421 acttatatag agataggtga cggcgacgag ctttgggaaa acaacaattt ttttaatatc
      481 aagcaggttc attgcaatgt tttctggctg ctgtccaggt tttacagtga gggcaggctc
      541 catatgattt atgggaatca tgacatggta aagaaataca ataggtttgt caggagcaat
      601 atgcatcggt atcttgatga gcctgcgaga aggcatatgc cgttatttat aaatataaaa
      661 ttccatgagg ggttgattct gaagtatagg cctacgggcg gcaagatttt cctgattcac
      721 gggcatcaag tggattgtct gaatgacaga ctgtggaagt tagccagatt tttagtcagg
      781 tatttgtgga ggcctcttaa taacctcgga gtaaatgacc ctacaagaac cgcaaaaaat
      841 tataaaaaga aggagacggc agaaaagaag cttaccgaat gggtaataaa ggaagggcat
      901 gtgcttatag ccgggcacac ccataggcca atgtttcctg aagtgaatga gcccccgtat
      961 ttcaatgacg gaagctgcgt acatcctcgc tgcattaccg gaattgaaat tgcagagggt
     1021 tatatccttc ttgtgaagtg gagtgtaaaa actaaggatg acggtacatt gttcataggc
     1081 agggaggtac ttgaaggccc aagaaagctg gttgattttt ttgcctgcaa agacagaacg
     1141 aaaaaacttt tgttatgaca gaatgaaata aggtataatt cagttaagat accttgagca
     1201 ggctaatgga aaggaatcaa ccatgaaaag attggcatat gtttttgtta tctctgcggc
     1261 agtgctgtgg ggtacgatag gaatatacag caatcaatta agccaatatg gagccaatgt
     1321 gctgcagata gtttcaatca gagctatttt tgccgcccct gcaatatttg tttttatcct
     1381 attaaaggat ataaagcttc ttaatattga tattcacgat tggaaatatt tttttggcac
     1441 cggtatttta agctttgtat tttttaactg gtgctatttt actgcaattg ataaaaccac
     1501 tttatctact gccgcaatat tattatacac tgcacctgct attgttatgg ttttttccat
     1561 aatattgttt aaggaaaagc tgacaagaag aaaggtcgct gccctgattc ttacctttat
     1621 cggttgtgca tttgttaccg gtgttgtaaa aggaagtcag gatatctcgc cactgggcat
     1681 attggcaggc ctgggttcgg gatttggcta tgcactgtac agtgtattcg ggagatatgc
     1741 gttgaagaaa tatgacccta taactgttac tctctatacc tttgtattcg ccagcattgg
     1801 tttaataccc ataagtgaca taaaggggct tttcggctta ttgagcagca ccgttgcctt
     1861 ttacaatgca gttttattag gcctggcagc tacggttatg ccgttccttc tgtacacaaa
     1921 agggctttcc tatttagaga caagcaaggc ttcaataatt gcagcaatag agcctgttgt
     1981 ggcaacaatt ataggaatca ttctttttgg tgaaccgatt accgtattta aaataatggg
     2041 agtattcttg gttgtatttg cagtgtctat tctggaacag aaaaaaagct gataagattt
     2101 attgttttat aagctcagtt tgctgacaaa gctcaaaata agaacagtaa tatatagtca
     2161 gagcatactt atacgaaaat tgttaatggc tcttatgagg ctcgacttag aaacaaccgt
     2221 atgcgaggca tggaggccga gcaagctgtc tcggtcacgg acgacctttg acagcgctag
     2281 gtttttctta gttgagcgga attagagcca tcaatttttg tatacctatg cgagactata
     2341 ccttactgtt cttatataga aggtttttca tcagtctcag ctgataagat ttgttatctt
     2401 atcagcttta tttcagaata ggatcctgct gctgacatct tcaatggaat tgcagccagt
     2461 cagtatcatt gcagatttta agtcagctgc aatgctgtca atataggctt ttaccccttc
     2521 tgcctgtccg ccgaatgaag ccggaacaaa aggcctgcca atcaaaacag catctgctcc
     2581 aagggcaagc atcttaagaa catctactcc gtttcgcacg ccgccgtctg ccaatatggt
     2641 gattttcccc ttcacttttt ttgcaatttc aggcaataca tccgctgttc cgggagtttg
     2701 atcaagtacc cttcctccgt ggttggatac cactatggca gctgcgcctg attcgacggc
     2761 aagctcagcc tcatccggag tcataattcc ctttaatatt aagggcagtt ttgtactttc
     2821 tgctatttcc tttatatcct caacggattt tggcccaacc ggcttgccaa gcagcgataa
     2881 agttattaac cctgctgcgt caatgtctat tccaactgca taggcacccg ctttttctgc
     2941 catgcgtatt ttttcaatga ccttatcatt tgtccagggc tttatgattg caattccttc
     3001 tccgcctgat ttttttagct cttcgagatt agttaccaaa agcgagtcta ttgctgcatc
     3061 tccgaacatt ggatagattc cgccctgaat acagcccttt attatccatg atatatattc
     3121 ctgttcctgg aatttccctc ccatattgaa attcgttcct gcaacaggtg ccgcaaaaac
     3181 aggtatactc atctttttcc caaaaagttc aatggaagtg tcagggtttt tacagttatg
     3241 caggactctc atattaagct tatatttctt taaagcatcg acatttgctg taaaggcttc
     3301 gctggttccc tttcctccca ttccgggcac ttctccggaa caagcttttc cgttgcatac
     3361 agggcagact ctgcatgtgc cgtttaaatt tgccttggca ttttttaaca cttcatcata
     3421 attcataatt cgtttacctc ttatcatcat gatatgttga tataaataat tcaggggctt
     3481 ttgcccctga attatcttat ttattaagag aaaggtttaa agtaattttt taagcatcta
     3541 cattctggtt cttggactta tctccggaga acgtctttat tgtagttatg attaatacta
     3601 ttgcaagtac gaaaagaatt gcggctaatg aaccgattat cacattgccg cccgcaaagt
     3661 tggttcttgc taccagtaca agggcagtaa gggttacgca gaacataaat accattggga
     3721 ttatagtcat agtattcttt ttccctgact tagccagcca agcagatact gcaaggagtg
     3781 caagtccggc aagcagctgg tttgcagaac cgaatatcgg ccatatcttc gtataaccgt
     3841 aggacagcaa taatgcagac aagcctacag ttacagcagt tgctgtatac atattgttca
     3901 gaggagattt tgttccatcc gccttggtag tcatctcctg gaacatataa cggcctaatc
     3961 ttgtcgcggt atcaagactt gtgagagcga atgcagagaa tgcaagtgtt acaaatacag
     4021 tacctacggg aacgggaata ccgaagctgc tcataaaatc tgcaacaccg gcagcgaaga
     4081 tctgagcagg cgtacctgtt gctccggcaa catagcttac cgcaataagt gcaattattg
     4141 caactacgcc ttcgataagc attcctccat agcctatcag ctgtgtatct ttttcattgt
     4201 ctacctgctt tgagcttgtg ccggaagcaa ctaatgagtg gaaaccggaa acagctccgc
     4261 aagcaacagt aacaaagagc atcgggaaaa gatactgggc cttcgcaccc tcaccaacag
     4321 taaagccgac tgaagccggg attgccatgg caggtcttac taaaagtact ccgattactg
     4381 ctccggcaag catagcataa agcaggaagg agcacaggta gtccctgggc tgcagcagca
     4441 gccaaaccgg cagtgttgat gctgaaaaaa tgtatataat aagaattaca tcccatgtga
     4501 gttttgaaaa tttcagtacc gggaactgat aacctatcca tatgcagaag aacagtaatg
     4561 ctacccctgc taccgtagat actgaaaggc tggctccttt tctgtaaaca aagtagccga
     4621 agcccattgc cagaaaaata aacaaaattg atgcagtacc tgcccttgcg ccggttattg
     4681 cctcaggctt tgccggatca aaagcaaagg ttccggcaca aatatctgta aaggctgcaa
     4741 caaccagtat taatgtaagg tatgcaaaaa tgttgaaaag ctttttgccg ttttcaccga
     4801 tatttgctct gatgatttca cctattgatt ttccgtcatg ccttactgat gcaaagagtg
     4861 aaccccagtc atggacaccg ccgacaaata tacttcctac tacgatccac aggtacaccg
     4921 gcagccatcc gaatactgca gcagctatag ggcctgtgat agggccggct ccagctatgg
     4981 atgaaaaatg atgtccaaga agtactttag cattagcagg acaatagtca atatcatccc
     5041 tcaatgtaca ggaaggtgtc ttcttagttg ggtcaattcc ccactgatta gccagccatt
     5101 tgccgtaaat ggtataggct atcagaaatg ccgcaatacc gataaataat aataccaatg
     5161 agttcataac acgacctcct tttttatttg cataggaaat attcatttcc ctaatgctct
     5221 gtttaatcaa aaaaaacagt tctgaaaagg ttttatttat tcaaagatgc aaattcatat
     5281 agttttttta cttttttccc gtccttgttg gtaataatct cgctattatc cagtcctaca
     5341 tatataagcc ttacatcaca ccaaccttta agatagaagc aaaacgcttt tttataatta
     5401 tacttttcta ctgctttaat tgcattttca tttatatgat taccgattaa tactcccgta
     5461 acatatgtgc tcatatggtc tttgtccggc tttatacatt catcagatat tctttttaag
     5521 aactgcccaa aagagataat gtctttctca tttacatcat cacatctctt gatatatatg
     5581 tgctcatgat tattatattg gtcaattata tcatgctggg taataaatgt ccttacactt
     5641 atatttgaaa acttggcaag tatatctatc ttattatcga atatcactat atccctttca
     5701 atggcaaaat tcttttcaag ctttgatgtc agcagcttta tatactcatc gtatttcatt
     5761 ataatcctcc caataaaggc ttcttgtaaa taatatgaaa tattccttaa ctctatacat
     5821 tcctacaact acattatata gggaataaat aaaccgtaat ttatttttgc ttgaaatgac
     5881 aagaataatg cattaaatgc aatcataggc gcataaaaaa actccctcaa ctgaaatcct
     5941 ctgacaatca gtgagggagt cttaaatacc cattagcttt ttaaataata atatgttctg
     6001 cctgctgacc ggaatctctg catccaaccc cttaagctta agcatatatg tattattgaa
     6061 ccaggggatt atttccgtta ttttatctat attaacgata taagaacggt gacagcggaa
     6121 aaaacaatct tgtcttagct ttttatgaaa atcggaaatg cttgaggcaa ctacaaagcg
     6181 cttgtccttt gtgtacacat aaacctcgtg cttatgtgcc tcacaataat aaatatcatc
     6241 tgcatccagc acatataatt tctcattttt ccaaagagtt attttatcgt tatgcagcct
     6301 aacagcagtg ctgttctcaa gtctttgaag cgtatctgct attcttgttt gggagtaggg
     6361 cttcaaaatg taatcaaagg cggaaacctc aaaggcttca agggcatgtt cgcggtaagc
     6421 ggtgataaat actagaacag gtttaaccgg caaggtgttt atcactttgc tgaaagttaa
     6481 tccatccagc ttaggcatat taatatccag aaaaataaca ttcactgaat gtctttggat
     6541 gtattcaagg gcttttatac tgtcatcaaa ggtttccgat attgtaattg aactaaaatt
     6601 tgtaataaaa taacttaatt cctgtatgga tgggtattca tcatctacaa taatacaatt
     6661 caaaaaatct cacccccacc tgaacataat aactgcaggc aatcaagcac tcatattaag
     6721 cataaaacta acccttgtgc cgacatccag gcgttcaata ttaagcccgg tgccatatat
     6781 atgacggagc cggttatcaa catttgatat accgattttg ttttctttaa cctttccggc
     6841 atgcaaattt tctatgaaag cctggggtat acctataccg tcatcctcta ctgaaaccct
     6901 gatttcatcc gggttatgct tttttacgga aattacaata tttccggtgc cggagccctt
     6961 cagaattcca tgttttatgg aattttcaac aattggctgt atgacaagtg aaggaatctt
     7021 tacatgaatg ctttcatcaa tatcatatat aacattaagc ttatctccga accgtgcttt
     7081 ttctacttca atgtatgatc tgacctgctg aagctccttg ctgatatcta caaaaatact
     7141 tgtttcttct atgttgtacc ttaaataggt tgaaaggttg acgattaatt ctctggcgct
     7201 attcggatta attcttaaaa gggctattat cgtgtttaat gcattaaaaa gaaaatgcgg
     7261 attgatttgg gcctgcagtg ccttaatttc agcttgtgca gccaattcct ccagcttgcc
     7321 gactttactt atttctattt gtgtcgatat taattgcgat aaacctatag ccagtttttc
     7381 atgggtaaag gatatcccgt ttctttttgc aaagtataat ttaagtgtac cgataatttc
     7441 atccttttct ttcagaggca ctataagccc ggacttcagc tgacagtctt ccgaagaaca
     7501 ctcaatttct tccggtgaat tcattatacg catctgacca ttcattatta cttgctttgt
     7561 agcaaaggtt tgtataggat caccgctgat atgatgatcc gaacccaatc ccacatgtgc
     7621 caggattgta gttttgtcag taattgaaac cgcagcagcg cctgtagatt cccttattat
     7681 tgtacatact ttttttaaag aatcaccgtt gatttccctg aaatacggga gggttttatt
     7741 cgctatttcg agagccaatt gagcttgcat tgcagccatt tgctcttttt ctttgaagct
     7801 attctgaatc agtaatatga ggatacatat acctatagca tttgtaaaac tcattgggac
     7861 ataaatgctt tttacaatgg caagtgcact ggaaaaaggc tttgacatga gcagaatcaa
     7921 cagcatttca atactttcta taatgatgcc cccggcaagc ccataaagcc atttctgata
     7981 tcggattcct ttcttataga tcaaggcgga aaggaaaccg gcaaggattg ttgatattgc
     8041 acagggaacg gaggttattc cgccgatatc atataaaaat ctgtgcaagc cggcaataat
     8101 acccgataca atacccacaa agggaccgca gagtatgcct ccggtcatta cgccgataat
     8161 tcgcgtatta gctatcgcac ctaatacgtc tgttcctgta tatgtactta taataccaaa
     8221 tacggcaaaa attacagata atatcagaag gtctgatttt ttaaatgcat ccttttgtat
     8281 tattttcttg aatacggaaa ggtttgaaac aataaaagct actataatta cataaattaa
     8341 actgtttatc aggttcttaa taagatcaat catttgatac ttcttaatct ccttttcata
     8401 tttttaaaaa aagcaaaata aggactatca atgagatatt ccttttataa cattttacct
     8461 ttttattctg aactttgcaa atagttttgg ggcaaggtat cccttgcccc aatgctttac
     8521 caaacctccg gaaaagcagc gccaacatat aaatcttcca atttagtctt atgtccttgt
     8581 tccattgctg caagtccaag gaatgtttcc ttttgttctt tatcggtact tatagctgca
     8641 aatcctttat acatatccat tgcttcttct tcccttttca ttgccagggc aatagcatca
     8701 gcaggcttca tatcaatgga aagcttaggc ttgtctactg attccgcaac cttgtagtct
     8761 gcagtcgagt cgaactctaa cttctttccg gtatctttaa aataatcttg aagtatgact
     8821 ttatgcgata attcgtcttt tgccaggttc tcaaatattt cagaaatgtt cttgtccttt
     8881 gtcttctttg caacatcccg atagaaggaa taagcttcct cttcgttttt tattgcatcc
     8941 gatattattt ttttgtattc ttcaagagtc attttacaac cctccctttg ttaacattat
     9001 aacataatta tacataataa atagtctttt ttaaaggctg aataactaat gctgatttgc
     9061 tttggtgcag gataccgggt gtaaaaccat acaaaaactc cagtatttta tacaacttac
     9121 ctaaaaatga taagatgcat caatatactg gagcctgagc gaaccatttt gaaatatatt
     9181 acttcttcaa ttcctgtttt ccggtgactt tgcttttatc atcaattttg aaagtggatt
     9241 tggcatcatc tgttgtaaaa tatacattcc cgtcgatggt tccatcaacc agctcaaagt
     9301 tctttgcaga aacataaaca tcacccttaa aggttccccc ctggattctg gcatttgggc
     9361 ttttgacagt aagctttggt gctgttagtg tgtatcttgc tgttacgttt ttgtctgcat
     9421 cctgcgcgta aagagctatt ttacgcttga gatccttagc cggatcgttc ttgtcatgga
     9481 attcgccttc caatataaga tcctcgttaa ttgacaaatc ctttgtagtg catattatcc
     9541 aggtgccgtt tccgcttata gcttttttaa aggcatcggc atcagctact attgaagcag
     9601 ttgttacaac atccggcgtt gcctcctgcg gcgttgcctc cggcggtgtt gtctgtggtg
     9661 gggtctcagg ggctgcaggc tccggagcac agcctgcaaa taatgcaata ataaccgcaa
     9721 atactaatac tatgactttt cctttcatcg gaaatcctcc ttgcatttat atatacttta
     9781 aattacatta ttgcctgaat gatagaattt aatgtatcac tgcaggccgg atacatgtca
     9841 acaaaaaata ttaattttga aaggaactgc caacaatata tttagcatta agattctttg
     9901 tcaaaaaaga ggaaaatttg attgcatgtc gaataagata gtggatatag gaccataggt
     9961 ctatatatat tcaataaata ggaccagaga actatatatt gattggatta ttacaagatt
    10021 ttgcaggagt ggattatatg caggaagcag caggtgcttt taaattatat atcgcaaaga
    10081 ataatgcagt tatgatacag gctgctgata atcaaaaagc agtgttttat ttttttatta
    10141 tacttctgat agcggctgct gttgctatta tacattatta cgcagccaga aatcggagag
    10201 ggactgtgaa cagcaaaaaa gaaggcgtaa ttagctatcg gacaataatt caacttatgc
    10261 aggagggatt tgcattattt gagataatat acgaccaggg cgggaagcca atagattatt
    10321 attttattga ttcaaatgag gcagctgagg ttattagcgg aattgaaagg aaaacgtcaa
    10381 ttaataaatc ggtttttgat attctgcctg attcggctgc agaattggca aaaaaattga
    10441 acgaagtcac atttgcagga gaaccatata aggatgaaat atatgtcaag gaatggggaa
    10501 agtatttatc agtaaacatg tataaggctg aaaaaaaact gcttgcagtt atgttttcgg
    10561 atattactca aaagaagctg gcggatgagg ccatgcagaa gcagaagctc agctatgagg
    10621 cattgtttag aaattcttcc gatgccatta tccggtttga ccagaatcat aacataattg
    10681 acataaatga gaaatttacg gaattgttcg gatataccct tgatgaaata tatggcaggg
    10741 agttagacag cgtattagct ccgaaagaaa agttcgatga ggcaaaagta ttgacaaaga
    10801 acgtgctcaa cgggaatatt accgctatgg aggaatcagt acgtttagga tctgacggaa
    10861 agaccagaga agtaagtata aaaggcgttg caattgttct aaacgataaa atcatcggcg
    10921 gctatggtat atatacaggt atatcccgaa ggaaaaaagc agaaagagaa ttaatgtata
    10981 tgaactatca tgacctgctg accggtcttt ataacagaag gttcttcgag gaggaattga
    11041 aacgcctgga tactgaaaga aatctgccga tttccattat tatggcggat gttaacggat
    11101 tgaagctgtt taatgatgcc ttcggccatg ctgaaggtga taagctattg gttaaagcag
    11161 ctgaaataat cagtaaggaa ttgcgggaag acgagatagt ggcaaggctg ggaggagacg
    11221 agctggttat tttattgcca gggactgatt cggaggaagc acgcagcata gtcaaaagaa
    11281 taagagcttc catttcaaac atacggctga aatccataga actttcaata tccttcggat
    11341 gggatacaaa aaacagtgcc gatcaggata tgaatgatat attgaaaaat gcagaagatt
    11401 atatgtacag gaataaatta tttgaaagtc ccaatatcag agagaagagg ataaaggcaa
    11461 taattaattc cttttatgag aataacagtg aagaagaaca gcactcaaag cgggtaagct
    11521 ccttgtgcga ggccaccggt gccgctatgg gcatggccgg agctgccctg aaggagctta
    11581 ggacattagg cttgcttcat gatatcggga aaattgcaat tgataatagt atactgaata
    11641 aaccggaccg gcttacaaaa gaagaatgga atgagattaa gcgccatccg gagataggat
    11701 atcggatact aagctcagtt aatgatatgg cagaattggc ggtattcata ctcgcacatc
    11761 atgaacgatg ggatggaaaa ggttatccga agggaataaa gggaacggac ataccgctgc
    11821 aggcaagaat cacagcagta gcagacgctt atgatgctat gacaagtaaa agtgcatacc
    11881 gaaatccctt gtctgaaagc attgcggtgg atgagcttga aaaaaatgca ggcacacagt
    11941 ttgatcctga aatagtgcaa atttttattg ataaggtaat aaataaaaaa gtcaaaactt
    12001 ctgttgacat tgacgttacg taaaggtgta tagtaatctt atcgggctca aggcggtgat
    12061 gatatggaat acacggtgca gaagctggga aaattagcag gtgtcagcac gagaaccctg
    12121 agatattatg atgagattgg aattcttaag ccggccagaa tcaatgcatc aggatatcga
    12181 atatatgggc agaaggaagt tgacaggctt cagcagattc tcttctacag agagctggga
    12241 gtgggactgg acagcataag ggaaatcgtg acggcgcctt cttttgacgg agctaaagca
    12301 ctgcgggaac atcgcgggaa gctgcttgag aaaagagagc agttggatat gctgattgct
    12361 aatgtagaaa aaaccatagc gttaacagaa gggagaatta gtatgaccga taaagaaaaa
    12421 ttcgaaggct ttaagcaaaa gctgctggat gataatgaga aaaagtacgg taaggaagtt
    12481 cgtgaaaaat acggtgagag tgcagtagaa aaatcttatg ataaagtaaa gaacatgacc
    12541 agggaagagt atgatgagct gacaaagctg tctgaggaaa taacaaaaat cttgggcgag
    12601 gcattcaaaa ccggtgaccc ggcaggagat ttggcacaga gggctgcgga tttgcacaag
    12661 cagtggctca cattctactg ggacagctat tcaaaggagg ctcacgcggg gctggcacag
    12721 atgtatgtgg atgacgagag gttcagagct tactatgaca aaaaacagcc cggcacggca
    12781 gaattcctga gagatgcaat tctcatatat accggaatga aaaagtagaa gcaataacct
    12841 tggaggacac ggggagagga cacggggacg tttcgaggac acggggacgt gtcctctccc
    12901 cgtgtctttt gttacagttt tattaaaaag caaagaagca gaagggcatt tgcataaact
    12961 tgtagaaata ccataaagag cataaatact acaaaattca tttattaagg agcaaattat
    13021 gaagtgtatt tcaaagttca tcatttttct gtttgccctt ttattaacat tatctgcatt
    13081 tccagtttcc gcagcttcgg aggccccgga gaaggaggaa ctcaggggag tatgggttgc
    13141 aacagtggcg aacatagatt atccttcaaa gtccactgcc gattcagcga tattgaaaag
    13201 tgaagccttg aaaatactgg atgatgctga ggccatgggc ttgaatgcga tttttctgca
    13261 ggtcaggcct gcttcagatg ccctttataa gtcaaagctt ttcccctggt caaagtatct
    13321 gacggggaga cagggactcg cacctgataa tggctttgat cctctggagt tctgggcagc
    13381 agaggcgcac aagcgcggaa tggaactgca tgcatggatc aacccttaca gaatcacaaa
    13441 aaagactcca aaggagccaa agcatgacct tgcttctctt gatccatcta acccagccag
    13501 attgaagccc gaatgggtgg tgaagcatac tgacggaaat ctttattaca atcccggaat
    13561 tcccgaggtc agaaagctgg taatagacgg ggtgcttgaa attatcgaaa attacggtat
    13621 cgatggtata catttggatg attatttcta tcccggaaag gactttagtg ataaggcagc
    13681 ttatgcaaaa tatggaacag cttatggcaa tatagatgac tggcgaagag aaaatgtaaa
    13741 caagcttatc agcgagcttt cgaaggccgt aaaggcggcc gatgaagctg taagctttgg
    13801 cgtcagcccc ttcggtattt gggcaaataa aagtgcaaat acgctgggaa gtgatacgag
    13861 aggcatgcaa acctactatg accaatatgc tgatacaagg aaatgggtaa aggagggctt
    13921 gctggattac atagcgccgc agctgtattg gtatataggc tatgaaattg cagattacag
    13981 caggctgttg agttggtggg ccgatgtaac tgcgggtacg ggtgttgatc tgtatatcgg
    14041 acaggcagca tatagaaccg gggatacaga tcctaaaagc ccatggtatg gtattgcaga
    14101 gatggaaaag cagcttaaac tgaattctga aaacaaggaa ataaaaggaa gtatcttttt
    14161 cagcaacagg tctttatgca ataacccggc tctatgtgcg gctttgcaat ctttatataa
    14221 aaggaagagc agtattccga tcagtgtttc acgtccggct gaagatatac agaccacctt
    14281 ggaaaaatat tacctgaacg gttcatctga tccggaaaag cctttgtatc tgaacggagt
    14341 acctgtggaa aacaggtcgg cccggggata cttcggagtt ttggtaccct tgacggaggg
    14401 tgcaaattct tttacactat cccaggaagc ctcagaggtg acccgtgtga tatacagaaa
    14461 aaccgctgat tccgaaccgg caaaaatgag cggggctgag ataatttcag cttcagtctt
    14521 tccgcaaagc caggagtacc gtaatcccgg ggaaaaaata actctttcct gcgaagctcc
    14581 ttatggctca acggtaacgg tgcagatagg cgggaaaaga tatttaatga aatcttcagg
    14641 cgcagtgaca ggaacctcag ggctgtatgc agacaaattt acctgcgaat atactattcc
    14701 ggcatataag ggcactcccc gtaatataga cctggggaaa ccggtttata caatgaatta
    14761 caagggtacg gccaagagct gtaccgcccc cgcaaagatc ggtgtgataa tgaaaggttc
    14821 accattctat gcaaaggttg aaaaagaggt catatataca tacaatgagg caagctcttc
    14881 agatggagca gcctatgagt tgtacagcgg aatggttgat tctgttaccg gaatgtcggg
    14941 gggctatgtc cgtctgtccc tggggcaatg ggtcaaggac agtgatgttg cggtatatac
    15001 ttccaaggcg caatcaaggc cgattataaa ggatactgag tatatatccg gggatgggtg
    15061 ggattcttta aaacttgata tatcggctcc gcctgccgct attgcagatt ttgacggagc
    15121 cctgctgaaa attaatgtat cagccgattc tcaggcagca tctcctgaac tgccggatga
    15181 ttcacctttt tcatctgttg ctgttgcaaa aaaagtgaac aatacggaat ataccatggc
    15241 attgaaaaaa aaccggagca taagcggcta ttatatgcaa aagacaaaaa caggactggt
    15301 gctgaatata aaaaggcctg taaaatcaaa taatagcaat gagcctcttt ccggcctgac
    15361 aatcatgctt gatcccggcc atgggggcag tgannnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnatgagcc tctttccggc ctgacaatca tgcttgatcc cggccatggg
    15541 ggcagtgata caggtgcaat aggccctctc ggaacagagt atccggaaaa gaccataaat
    15601 ctgaatattg catttaaatt acagtcggag cttgagcagc ttggggcaag ggtatttctg
    15661 acaagagcgg cagatgtgaa tgtgtccctg gaagagcgcc ttgcagcctc cagaaatgca
    15721 aagcctgata tgttcctttc tcttcattcc aatagcatgg cggaggatgt tgatatatcc
    15781 aaagtagacg gatttgcagt tttctaccgg gaaaaacttg cactgccatt ggcagaaaca
    15841 atattccaaa atacattgac acatctgaac agaaccaaca aagggctgca taacaagaat
    15901 ttttatgttt taaggggtac ctggacacca tctatccttg ttgaaagcgg ttttgtcccg
    15961 aacccatatg aatttgaatg gctgattgat gagaatgagc aggccgggct tgcccagagc
    16021 atagcgaaat ctgtagttga atatttcaag taagtaatga acatactctt ttttttgata
    16081 atttgataga ataagaatat agctgaagca ggaggcaggc ccatgtcgga actcagaaaa
    16141 atgaaagcaa tagtattatt aagtatcgca attatgacgg ctatagtgat tttacccgca
    16201 agtgccgcag ttggacagca gcctcctttt tctgaagtca aggaaaagct ttcgggaatt
    16261 tctaaagagg aaaaggagat tttgcagaaa ctgttcatac ttgctcagga gattgaaata
    16321 gcggaggcgg aggcagagga gctatcccgc gaaacagaag caatcagtaa agaaataaaa
    16381 ggcttggaag aggatatagc aaaagaggag cttgcttatg aaaagaagcg gaagagcttg
    16441 gagcaggttc tgaaaagcta tcagaggatg ggagccgggt cattcctgga aatcatcctg
    16501 gactcggaca gcctgagtac ttttttgcat aggttgaaca tactccggga tcttaccaaa
    16561 aataccggaa cgcttttgga gcagctggag tcaagcggca ggaagcttgc agaggaaaaa
    16621 tcaaaactat cggaaaagct tgcattggtt aaagataagg aaagtatgtc aagaacggcg
    16681 ttagctgata aaaacaggct taaggcggaa aaggaggcat acctggcatc ccttaaggga
    16741 gagaaggagc attaccagga atacctttcc aatctggaaa gggtctggaa tgagcttaag
    16801 cctttgcttt cagagaccgc aaaggaattc tcccgaatga ttgaagagga gggattgcct
    16861 tatgatgctt taaaaatcag cttttctctt tttgaagtca agggagctat tgacgatggg
    16921 actataaaca aaattatttc agagaatgcc aatcttgaag aaatgatttt tgcttttcat
    16981 caggaagcgg ctgaaataag gatacctgat aagaacctta tattgacagg aacttttgca
    17041 attcaggaag gacacactct taagtttcag gcccgggaag gaagcttttt cggattgcct
    17101 ttggaaaagg gagttctgga ggaattattc ggagaggaag gcatgatgct gaatcttgag
    17161 ccgctgctgg caggaaacga tatacatgcc gtaagaatta aagaagggta catagaactg
    17221 attcatagat tttaatttgt tattgtattt ggaggggaaa taatgataga agctaaggga
    17281 gtatgtttgg agtatccgga tggaaccaaa gccattaaaa atattgagct ttcagttaaa
    17341 ccgggtgagc ttgtatacat aaccggaccc agcggctccg gaaagacaag cctgctgaag
    17401 cttttcatgg gtgaggaata tccgactgcc ggttccctga gggtgctggg acagcacata
    17461 tccaggggac aagcgtcaag aatcagaggc ctgcgcagaa tgatcggtcc ggtttttcag
    17521 gacttcaggc tgataaaagg gagaactgcc ttggaaaatg tcatgctggg aatgagattt
    17581 ctggatatac cttccggctg cataagggaa aatgccggaa atgcactgaa aaaggtgggt
    17641 ttggagcaaa aaatgctttc actggtagag cacttgtcct ggggcgaatg ccagagggtg
    17701 gcaattgccc gggcagtatc aagagaaccg gtcttgataa tagcagatga gcctacgggc
    17761 aatcttgata aggacaatgc cctaaacata ctggaattgc tgaaatcctt cagaaacgac
    17821 aataaagcca taatcatcac tacccatgca acccatttga ttgaagatga aagatatgca
    17881 gtacatatcc ggatggataa cgggaacatg ctcaaagaaa ggtcagggga agcttagatg
    17941 aagaatattt tgtacaatac cggttatttc attaaagaaa caaagacaat aatgcgtttg
    18001 aacctgctgt caagtatttt atcttttcta agcatcgggc ttattttttt catcctcgcc
    18061 atgatgattt ccggctggtg ggtaagcagc catgttgtgc ttgccataca agaagaggct
    18121 gaaataagta tatatttcag tgaggatatg gacgctgccg gtgcagaaag gctggccgaa
    18181 gagattgaaa acattgaggg agtatatgaa gcaaggctgg tcagcgaaga ggaagcctac
    18241 agcaggatgg aaaaaatact ggggaaggaa gcccgtgttc ttgagtattt agatgataat
    18301 cctttcagtc cctttataga agcaaaaatc caaatagaag atataggcct gataccgggg
    18361 aaactagatc tgattgaggg cattgagcat gtgagggata acagggaagt gctggaacgc
    18421 atcctcggga tttcacagac aataaagata ttgggatacc tgattatagc cgctgtcggg
    18481 atatccacaa cggttataat atcccacatt atcagaatgg gaatatacga ctgcagagaa
    18541 cagataaata ccttaaggct gatgggagcg cctgaagctt ttatcgggtt tcccttcatg
    18601 ctggaagggc ttcttctcac tctgggagga ggcgcgttat cctcagcctt ggcagcatat
    18661 gcattaaagt atttgtatgc acaggcagcc ggcccgatag ccttcattcc tcttccgccc
    18721 cgggaggcat tggtttctga ccttactata ttgattatgt tattgagtac cgcattggga
    18781 acagccggaa gttttttcgg tttgtcatcg gcaaaaaact gaacaagaaa acgcacatgg
    18841 gcggatatca taatctttgc atatttatgg tataattaat gcataacaaa ggagggaatg
    18901 caaatgagtt ttaaaatgac cggcaaagtt acctgggtag ggaaaattga ctgggagttg
    18961 aggagatttc acggggacga gtattcaact cacagaggtt cttcttacaa ctcatatctt
    19021 ataaaggaca agaagaatgt acttattgac acagcttggg caccctttgc aaaggagttt
    19081 gtggaaaacc ttaaaaagga gatagacctg gataaaattg actacataat tgcaaatcac
    19141 agtgaaattg accacagcgg ggcactgccg ctgctgcttg cggaaatacc aaatactccc
    19201 gtttattgca cagctaatgg ggccaagata ttaaaagggc attatcataa ggactggaat
    19261 tttgtaacgg taaaaaccgg agataccctt gatattggtg aaagcaagct tacttttgtg
    19321 gaagccagga tgcttcactg gccggacagt atgttcgtgt acatgtcagg tgaaaacata
    19381 ttgttcagca atgacgcctt cggacagcat tatgcctcgg agctgatgta caatgatatg
    19441 gtagacaatg ctgaattata tcaggaagca attaaatact atgcaaacat attgaccccc
    19501 ttcagtacct ttgtcaccaa gaaaatagaa gaagttttaa gcttcaattt accggtgaac
    19561 ataattgccc ccagccacgg gataatctgg agggacaatc cggcccagat aattcagaaa
    19621 tacatggatt gggcaaagga ctatcaggaa aatcaaattg taatagtata tgatacaatg
    19681 tggaacggta caaggaggat ggccgaagct atagcggaag gaatcagatc ggttgataag
    19741 gaggttgcag taaagctgtt taatgctgct cattccgata aaaacgatgt gataacggaa
    19801 atatttaaat ccaaggcagt attgttcggt tcttccactg taaataagag tattatgcat
    19861 gcagtgggcg ggatgttgga tatggtaaaa ggcttgggct tcaagaataa aaaagcagca
    19921 gccttcggct catacggctg gagcggggaa tcggtcggta tattgacgga aagattgaag
    19981 gatgccaaat ttgaaatagt aagcgacgga ataaaagaac tgtggaatcc ggaccaggat
    20041 gcaatgaaca gatgcgtgca gttcggagcg gattttgcag ggaagctgta gtccggaact
    20101 tttcgcaaag atgctgcgtc ataactgtgt acggaaaaat atgcagtgtc tttgaaggga
    20161 gagttatgct aataaaaatg tttaataaaa aagctttttt caggtctgct tcattagtta
    20221 tgtccattgt tgtgcttctg ggtgcaggat tgctgacagg gtgcacggca cctgcagagg
    20281 ataatacagg cggcaaagct gaggataata accctcctgc tgtggaggaa agcattgagg
    20341 ataggattat tcccgagttt atggcattgg cggtggggaa tcccaagcct gatgcaataa
    20401 ttgagttcat ggataagagt attgctgacg tttccggaga aaatgcgtct aaaatgcttg
    20461 atgaacttga aaaatccctg aaaagcaatt taccggagct ggaggagaaa tattacagca
    20521 cggcggtaca agaagcaatg ttcaatgcgt acaaatcgga atttgatcta agcaaacttg
    20581 acagcattca agatgctgaa gttaaaagcc ttattgatga aaccaaggct atgggctata
    20641 aggtggaaac agcggaaggc atgtttttcc ctattattaa ctatgaattt cttaagaaat
    20701 tcagttctta tgcagaggat gacatgaagg attatattga tataatggca gcagagagca
    20761 ataaagttcc cgcaaaggat ggggctcttg tgataggctg ggatgaggta attgagaggg
    20821 cgttggccca ggagaacttt atggcgaaac acagcagctc tgccaaggtt ggaagcataa
    20881 aggaactgca gaaaaaatat atcatgttca ttctgtacgg attgaataat acccctctat
    20941 tcagctatga tacaaaaatg atgaatcctg aagccaaaga tgcttataca aaggttttga
    21001 aggaaaaagg agatagtgag ctgttgcagc ttttgggc
//
LOCUS       DAOOAB010000044        20972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3338_length_20972_cov_17.829182, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000044 DAOOAB010000000
VERSION     DAOOAB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20972
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_20972_cov_17.829182"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..1404)
                     /locus_tag="PKU88_09420"
     CDS             complement(541..1404)
                     /locus_tag="PKU88_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPX69533.1"
                     /translation="MQQYILSSFVSNRGGGNKAAVVFDDFGMSYWQMLELAKKNNFSE
                     TAFIDFNKSDGNRFSIRYFTPAEEVDICGHAALASFQVLKQLGYLRDNYAYHRTKAGE
                     LKVLIEGDSILIQMNKPELIMQLSSNIIMDIIDAEKDEITTNRNGMIDVISTGLKDII
                     LEVNGIEALKKLRIKKDKMIEICREYDIIGMHVVSRETIEEDSDFCCRNFAPAVGIDE
                     ESATGTSNASLLYYIKRNDKQFDCGKTYKVEQGYFMNSPSNIFVKYDQGNESIFVGGI
                     ARIDSMESVVI"
     gene            complement(1519..2361)
                     /locus_tag="PKU88_09425"
     CDS             complement(1519..2361)
                     /locus_tag="PKU88_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPX69534.1"
                     /translation="MISRRGLGKGLGALIPEGEESDRNSIVEIKITDIEANDKQPRKA
                     FNDATLADLSESIKEHGVVQPIIVRKQGNGYQIVAGERRWRAARLAGKKTIPAIIKEC
                     SNLEVMELALIENLQREDLNSIEEAMAYKSLIEEYNMTQEEISKKIGKSRPAIGNSLR
                     LLQLPQEIKNMIAEGKISQGHARALLSISGEKKQLDMAEKIIAQQLNVRQIEKLAKDT
                     KQKKKKEVLPDAYRIEINQLEERLRAVLGTKVTIHHRKNRGSIEIEYYSDEEFDRIFE
                     LLEK"
     gene            complement(2362..3135)
                     /locus_tag="PKU88_09430"
     CDS             complement(2362..3135)
                     /locus_tag="PKU88_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX69535.1"
                     /translation="MSKSIAVFNQKGGVGKTTTVINLCACLANRGKKVLMIDVDPQGN
                     TTSGVGIDKTTLEKTIYDVLINGSDPRECIIDTNRENLFIIPSSVQLAGAEIELTEMN
                     KRELKLRECIESIKDEYDYVFIDCPPSLGLLSINALSAVDSVLIPIQCEYYALEGVSQ
                     LMNTIKLVKRSLNPDLEIEGVVLSMFDGRTNLSIQVVDEVKKYFRGKVYTTMIPRNVR
                     LAEAPSYGMSIIEYDMKSKGAESYMDLADEFIELSEDVI"
     gene            3407..3676
                     /locus_tag="PKU88_09435"
     CDS             3407..3676
                     /locus_tag="PKU88_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HPX69536.1"
                     /translation="MLKSVLYTFFLVFIAELGDKTQLATMLLSAKSNSIIAVFIGASF
                     ALICSSFIGVFAGAYITKHVPPQYIQNAAGVLFIAMGLLILKGKI"
     gene            complement(3717..4511)
                     /gene="noc"
                     /locus_tag="PKU88_09440"
     CDS             complement(3717..4511)
                     /gene="noc"
                     /locus_tag="PKU88_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HPX69537.1"
                     /translation="MNDRKEIINIPIDEIAPNPYQPRKEFNGSSLEDLASSIKEYGVL
                     QPINVRKIGEKGYELISGERRLRSSKLAGKSHIPAVIIEVVEQDSAVIALIENLQRED
                     LNFIEEAEGYHNLINDHGMTQEELARKVGKKQSTIANKLRLLKLKDSIKKTILENELT
                     ERHARALLKLPDDTLQEKALKSIIKKSLNVKKAEELVDKMLDEAAAAAEEPKKKRIKG
                     KMNYNIYVNTLKNACKEFLTAGCKVEYGQVDKGEFIEITVKIPKNV"
     gene            complement(4644..5363)
                     /gene="rsmG"
                     /locus_tag="PKU88_09445"
     CDS             complement(4644..5363)
                     /gene="rsmG"
                     /locus_tag="PKU88_09445"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPX69538.1"
                     /translation="MENRKILIDGLTELGIEPANKKVDKLVAFKEIMLEWNEKVNLTA
                     ITEEKEIFIKHFLDSATCLSAGYIKEGMSIIDVGTGAGFPGIPVMILMDGLKMTLLDS
                     LNKRVIFLSEAIKKLDLRDITAVHGRAEEAGGNKAYRECYDIVLSRAVASMNVLCEYC
                     IPFAKIGGYFLCQKGPEIEEEIGNAASAVKILGGTIKEVKEYKLPFSDIRHNIIVIEK
                     VSATPTKYPRKPGKPASNPIT"
     gene            complement(5338..7251)
                     /gene="mnmG"
                     /locus_tag="PKU88_09450"
     CDS             complement(5338..7251)
                     /gene="mnmG"
                     /locus_tag="PKU88_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPX69539.1"
                     /translation="MEYTAGKYDVVVIGAGHAGSEAALACARLGLKTLVTAINLDSIA
                     MLACNPSIGGPAKGNLVREIDALGGEMGINVDRTMLQIRMLNTRKGPAVRALRAQIDK
                     KLYQDRMKRVMENQENLQIKQAEITRIIVENDRVKGVVTHLGAIFECRSVILATGVYL
                     KGKVVIGEISYDGGPNGLFPAAKLSDSLEDLGIKLLRFKTGTPARIDGNTVDFERMTE
                     QPGDRDINTFSFMTGDIDIDQLSCYLTYTNKGTHDIIRKNLHRSPLYTGDIVGVGPRY
                     CPSIETKIVSFPDREEHQIFIEPEGLNTREMYVQGMSSSLPEDVQLDILHSVKGLENC
                     HMMRTAYAIEYDCIDPTQLQLSLEHKKISGLFFAGQINGTSGYEEAAAQGLIAGINAA
                     LSLRGKEPFILDRSEAYIGVMIDDLVTKGTNEPYRLMTSRAEYRLILRQDNADLRLTQ
                     KGYDIGLVSEERYKRYLEKKNNIEKETDRLKKKSISMTEDIREFLMSRESAEIKGGIS
                     LYELLKRPEIEYCSLKEIDEEMPELDRETAEEIEIMIKYEGYIKKQLQQAEQFKKLES
                     RKIPENINFADIKGLSLEARQKLDSINPANLGQASRISGVSPADVSVLMVYLEQIRRG
                     RGGEQAGKQENTD"
     gene            complement(7262..8641)
                     /gene="mnmE"
                     /locus_tag="PKU88_09455"
     CDS             complement(7262..8641)
                     /gene="mnmE"
                     /locus_tag="PKU88_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPX69540.1"
                     /translation="MFERTIAAIATAPGEAGIGIVRISGEKSTEILKKVFKPKRVMDI
                     DDIPSRTVVYGHAVDSKGAIIDEVLVIVMRKPYSYTMEDVVELHCHGGIVPIRRIMEE
                     VLRNGADIAEPGEFTKRAFLNGRIDLAQAEAVIDVITAKTETGLNAALNQLEGELSKE
                     IKKIMEKLLSMLAHIEASIDFPEHDVEEITTANIKNMLLEAKASVMELAESFDEGKIV
                     RDGLSTAIIGRPNVGKSSLLNVLLKENRAIVTEIPGTTRDIIEEYLNMGGVLVRLIDT
                     AGIRDSEDIVERIGVERTKGAISNSDLIILVLDASDELRQEDNEIIDLIKNKKVIAVL
                     NKTDLGLVIDEAYLRGVFGEDNIIRMSVKNRIGIDKLEERIKGLVYHGKLSISKNSMV
                     TNIRHKNLLDRALENIERAILSIEDNIPVDLISVDIREAWNSLGAITGDTVEEDIIKE
                     IFSKFCIGK"
     gene            complement(8927..9619)
                     /gene="jag"
                     /locus_tag="PKU88_09460"
     CDS             complement(8927..9619)
                     /gene="jag"
                     /locus_tag="PKU88_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPX69541.1"
                     /translation="MMMVEKTARTVEEAVQSALEELETEKDNVDIEILEQPTKGIFGI
                     LGSKNAKVRVTLKEKKTTEKSVKKEIENKEIRITEYTLEKERARKFLREVLEAMDIKA
                     EIRVKNINESLYINLSGPKMGMIIGRRGQTLDSLQYLVSLVVNKDKERDSFVKVILDT
                     EDYRRKREETLQRLAKRLADRARKTGKNIELEPMNPYERRIIHSTLQEVEGITTFSEG
                     EEPYRKVIISNK"
     gene            complement(9625..10353)
                     /locus_tag="PKU88_09465"
     CDS             complement(9625..10353)
                     /locus_tag="PKU88_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPX69542.1"
                     /translation="MLDFIAEPLGALLKFIFDFVGDYGYSIIIFTILTKVLLLPINIK
                     QTESTKKMNELNPKMKEIQEKYKNDKEKMNQKLLQLYKEQNYNPASGCLPALIQMPIL
                     FALFYVIQNPVKYVFKDANIYAGIAKNFWWLLDLGKPEMQATMIKVAGFGVPILAVLS
                     AATTYYQMKMISAPKANKKKGKEDTAAQTQSMMTNIMPIMFGWITLNVPSGLALYWVA
                     GNIFTIIQQYFMMKPKPQAAKKEE"
     gene            complement(10451..10675)
                     /gene="yidD"
                     /locus_tag="PKU88_09470"
     CDS             complement(10451..10675)
                     /gene="yidD"
                     /locus_tag="PKU88_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPX69543.1"
                     /translation="MRKILIVIIKTYQLLISPLSRGKCRFIPTCSSYAIEAIDRHGVI
                     RGLYLSAGRILRCNPFNPGGYDPVPEKKNR"
     gene            complement(10676..11020)
                     /gene="rnpA"
                     /locus_tag="PKU88_09475"
     CDS             complement(10676..11020)
                     /gene="rnpA"
                     /locus_tag="PKU88_09475"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPX69544.1"
                     /translation="MTTLHKLKKNYEFKKVYNEGKYYVEKFVVMYIIPNDSNFNRVGF
                     SVSKKVGKSVIRNRIKRLMKEAYRKFADTVKLGYDIVFTARAGSGSADYSMIENNIIS
                     ILKRAKLKKSEE"
     gene            complement(11068..11202)
                     /gene="rpmH"
                     /locus_tag="PKU88_09480"
     CDS             complement(11068..11202)
                     /gene="rpmH"
                     /locus_tag="PKU88_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPX69545.1"
                     /translation="MLMTYQPKKRQRKKVHGFRERMSTATGREVLRRRRRKGRKRLTV
                     "
     gene            11727..13058
                     /gene="dnaA"
                     /locus_tag="PKU88_09485"
     CDS             11727..13058
                     /gene="dnaA"
                     /locus_tag="PKU88_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPX69546.1"
                     /translation="MYSQLVEIWEKTLSVIKVELTEVSFNTWLKAIQPVSIKENLITF
                     SVPDDFTKGILEARYSKLIKNALKQVTSEYYDIRLIIPTEENFTSIMTERSEINQSEE
                     SVNSSLNPKYTFETFVIGNSNRLAHAASVAVAESPAKAYNPLFIYGGVGLGKTHLMHA
                     IGHYILKQNMRSKVVYISSETFTNELINSIRDDRNNEFRSKYRNVDVLLIDDIQFIAG
                     KESTQEEFFHTFNSLYEANKQIIISSDRPPKEIRTLEERLRSRFEQGLIADIQPPDLE
                     TRIAILRKKAKSENLSISDDVMLLIAKKIHSNIRELEGALIRVVAYSSLTNGEINEEL
                     ANEALKDILSNSKPKKVTPSAIKDVVGNYYNIKIEDFNAKKRNKSVVLPRQIAMFLCR
                     ELTDLSLPRIGEEFGRDHTTIMHAYEKISSDIKIDTQLKSVLEELKSKIEG"
     gene            13287..14393
                     /gene="dnaN"
                     /locus_tag="PKU88_09490"
     CDS             13287..14393
                     /gene="dnaN"
                     /locus_tag="PKU88_09490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPX69547.1"
                     /translation="MKIITTKSTLLESINVVQKAVPSKTTLPILEGILFDAKSGKLKL
                     TATDLEMGIETTSNVDVIEPGKIVLSSRMVGEIVRKLPDSDVEIETAEGNLVSIKSEG
                     SKFKIVTLPYDEYPELPVVKKENGITIKQNILKDMIRKTIFAVSTDEIRPILTGVLFD
                     VSGEELTMVALDGFRMAVKKNVIMNDNNFKAVIPGKTLSEIGKIINDNEENINIYFSK
                     NHIQVQIEDTIIISRLLEGEFINYKQIIPAEYKIKTKVSTNLLMEACDRAALFARDSS
                     NNMIKFEINDDMMVIMSNSQNGDVHEELKIQKEGSDIEIAFNAKYFIDVLKVIESEEI
                     TIEFTTNVSPSIIRPVKDEEYLYLVLPARFKKNS"
     gene            14462..14668
                     /locus_tag="PKU88_09495"
     CDS             14462..14668
                     /locus_tag="PKU88_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPX69548.1"
                     /translation="MREAKIETDFIKLDQFLKFENIAGTGGEAKNMIMDGLVKVNGNI
                     EKARGKKLRRGDVIEIFGEEIKLI"
     gene            14690..15796
                     /gene="recF"
                     /locus_tag="PKU88_09500"
     CDS             14690..15796
                     /gene="recF"
                     /locus_tag="PKU88_09500"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPX69549.1"
                     /translation="MYIKRIDLKNFRNYKEFDIELGKGVNIFYGQNAQGKTNIIESIY
                     MASTGKSHRTQKDMELIRWNSEDAKIRIDYQKKEEEKIEIYLNKSLKKQIKKNGVRLF
                     KIGELIGNLNTVIFSPDHMKIIKEGPAERRRFIDIILSQVKPGYYYNLVQYLRTLEQR
                     NNLLEEARTNSGILKTIDIWNEQLADFGARLIKARQDFICDICCMAADIHKKISNGKE
                     TLVIKYNSSIKYHSNSEKEIKENFIIALENHINADMGRSATHKGPHRDDILFFINGTE
                     VKTYSSQGQQRTTLLSLKIAELKYMKEETGELPVLLLDDVFSELDDERQKYLIEFIKD
                     MQAIITCTNVEHLEKLMLEGSRTYKVESGMIVKD"
     gene            15878..16147
                     /locus_tag="PKU88_09505"
     CDS             15878..16147
                     /locus_tag="PKU88_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPX69550.1"
                     /translation="MFMHLGGDVVISLKDIISIMDIESSSTSSITKEFLKTAEDEGFI
                     RRISEDEEKSFILTEKNKKTIIYLSPISSVTLFKRAGFIKDISLK"
     gene            16161..18071
                     /gene="gyrB"
                     /locus_tag="PKU88_09510"
     CDS             16161..18071
                     /gene="gyrB"
                     /locus_tag="PKU88_09510"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPX69551.1"
                     /translation="MEENKFEGTYEAEQIQVLEGLEAVKKRPGMYIGSTGSRGLHHLV
                     WEVVDNSIDEALVGACKNIKVTIHPDNSISNEDDGRGFPVGMHPKLNKPAVEVALTVL
                     HAGSKFGGGGYKVSGGLHGVGVSVVNALSEWLEVEVKRDGKIYHQRYERGFPVTELKV
                     IGTTNRTGTRVSFKPDSDVFDELEYSFDILEHRLRELAFLNKGIKISLKDERDGTEKT
                     FQYEGGIISFVKHLNKNREVLHKEPIYFEAKKDNVDVEIALQYNDGYAESIFSFANNI
                     NTLEGGSHMSGFKNAVTKVINDYARKYKFLKENDANLIGEDVREGMSCVISIRLTDPQ
                     FEGQTKTKLGNPEVRGIVDSIVTENFEAFLEETPSNAKIIVEKALTAARARDAAKKAR
                     ELTRRKNVLESTTLPGKLADCSERDPSLCEIYIVEGDSAGGSAKQGRDRRYQAILPLR
                     GKILNVEKARLDRILNSEMIRAMITAFGAGISDDFNLEKMRYGKIIMMTDADVDGAHI
                     RTLLLTFFYRYMSPLIEAGYVYIAQPPLYKVRKKNKDYYAYNDKELEKVFKEIGREGS
                     SIQRYKGLGEMNPEQLWDTTMNPETRIILQVTMEDAVAADEIFTVLMGDKVEPRREFI
                     EHNAKYVRNLDI"
     gene            18095..20536
                     /gene="gyrA"
                     /locus_tag="PKU88_09515"
     CDS             18095..20536
                     /gene="gyrA"
                     /locus_tag="PKU88_09515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPX69552.1"
                     /translation="MDYENQKILPINIEEEMKKSFIDYAMSVIVQRALPDVRDGLKPV
                     HRRILYAMNDLGITPDKPHRKCAMTVGEVLGKYHPHGDSSVYDAMVRMAQDFSQRYML
                     IDGHGNFGSVDGDGAAAMRYTEARMSKIAMELLKEIDKETVDFTPNYDESLKEPTVLP
                     ARFPNLLVNGSNGIAVGMATSIPPHNLGEVIDCAVSTIENPDIELEEMLKYIKGPDFP
                     TGAIIMGKEGIKKAYSTGRGKVIVRAKAEIEEMSGNRQRIIVSEIPYQVNKAIMIEKI
                     ADLVKEKKIDGISDLRDESDRNGMRVVIELKRDANANIILNQLYKHTQMQETFSIIMI
                     ALVNGQPKVLNLKSVITHYIDHQKDVVTRRTQYDLKKAEARAHILEGLRIALDNIDEI
                     VSILRNSKNVAEAKERMMSRFGLSEIQAEAIAEMKLKSLTGLERGKIEEEYLSIIKNI
                     EYFRSLLANEQKLLMVIKDELLEIKKKFNDKRRTKIIPTSLEEFDEEDFIEEHEVAVT
                     ITHFGYIKRLPADTYKSQRRGGRGITGLQTREDDFVENLFITSTHNYILFFTNKGKMY
                     KLKTYEIPEAGRQAKGTALVNLLQLDAEEKVNAVIHIKDFNDGKYLIFMTKHGTIKKT
                     ELKEYDTSRNTGITAINLIDNNELIGVKLTRGTTELIAATKKGLAIRFPESDVRPMGR
                     SARGVRGINLNRDDVVIGMDIVEEGADLLVISEKGYGKRTPIDEYRVQTRGGKGIITS
                     RITPKTGHVVGMKIVKEQDEIMLINLSGIIIRMNVNEISKMGRSTQGVKLMRVPENES
                     VVSIAKISSNEEEEK"
BASE COUNT         6454 a         4254 c         3806 g         6458 t
ORIGIN      
        1 gacagaatca gagcttgata cttgtaaaat cctgacgcac ataaacccaa cctccaggtc
       61 ttttgcttga caaaagacta catatattcg tgggcatagc ccacatgttg gtatgtgctg
      121 gatttacctc acgtaaatcc cacggatttt actgcgtatc ttctctcgat tctgttgccc
      181 aaagttaata agtgctcgct gttatacaag gctattgccc tgaaagttga atacaaagca
      241 agggcttgta tagtatgata atattatgga aatgcaaata ccgacgtggg gaagatatct
      301 tcgacggaac tttccacccg gcctcggaac gggaataccc cagaggaaaa gctagtctca
      361 cgacgctttt ccgaccctag ggaaccctat ggttcccgaa ggcgttagca agcatcctga
      421 gcaccctcct ttatcggcag ggggcttctc ccctttgcat cccccgcttt ggaaggtgag
      481 gagaacatac ttcccctaca gtcggagtac ttcacaaacc cttgtttttt agcattctta
      541 ttatatcaca acagattcca tactatctat cctggctata ccgccgacaa atatactttc
      601 atttccttga tcatacttta caaaaatatt gcttggactg ttcatgaagt agccctgttc
      661 aaccttataa gttttgccgc agtcaaattg cttatcattt ctctttatat aatatagcaa
      721 tgatgcgttg gaagtcccgg ttgcgctttc ttcatctata cccaccgccg gagcaaagtt
      781 ccggcagcaa aaatctgaat cctcttcaat tgtttcacgt gaaacaacat gcattcctat
      841 aatatcgtac tctctgcaga tttctatcat tttatccttc ttaattctca atttcttcaa
      901 tgcctctata ccgttcactt ccaatataat atcttttagc cccgtagata taacatcaat
      961 cattccgttt cggtttgtag ttatttcatc cttttccgca tcaataatat ccattattat
     1021 attactgctt aactgcataa tcagttcagg cttattcatt tgaatcaata ttgagtcccc
     1081 ttcgattaat accttaagct ctcctgcttt tgtccgatga tatgcataat tatctctcag
     1141 atatccaagc tgcttcagta cctgaaagga agcaagggct gcatgtccgc aaatatcaac
     1201 ttcttctgcc ggtgtaaaat atctgatact aaacctgttc ccatcacttt tattaaaatc
     1261 aataaaagcg gtctctgaaa aattgttttt cttcgccagt tccagcatct gccaatatga
     1321 catcccaaaa tcatcaaaaa caactgctgc tttattgccg cctcccctgt ttgatacaaa
     1381 ggaactcaag atatactgct gcatatatca aaacctccat atacccatta atgttatcta
     1441 taattaattt tttgatttca taatataaaa ccattatggc acaaaaaata atcgattata
     1501 aaccatttaa ataaaccttt atttttccag taattcaaaa attctatcaa attcttcatc
     1561 actataatac tcaatttcta tactgcccct atttttccta tgatgtatag tcaccttagt
     1621 acccaatacc gcccttaatc tttcttccaa ttgattgatt tctatccgat atgcatcagg
     1681 caatacttcc tttttctttt tctgctttgt atccttggcc agcttctcaa tctgtctgac
     1741 atttaattgc tgggcgatga tcttttctgc catatctaat tgcttctttt cccctgatat
     1801 tgaaagcaaa gcccttgcat gcccctgaga tatctttcct tccgcaatca tattcttgat
     1861 ttcctggggg agctgaagca gcctcagaga attgccaatt gcaggcctgc tctttcctat
     1921 tttcttcgat atttcctcct gcgtcatgtt atattcttca ataaggctct tataggccat
     1981 agcctcttca attgaattca aatcctccct ttgaagattc tcaataagag caagctccat
     2041 tacctccaga ttgctgcatt cctttataat tgccggtata gtcttcttcc cggccagtct
     2101 tgcagctcgc catcttcgct ctcccgcaac aatctgataa ccgtttccct gttttcttac
     2161 aattataggc tgcaccacac catgctcttt aatagactct gaaagatccg ccagtgtcgc
     2221 atcattaaat gcctttcttg gctgcttatc gttggcttct atatcagtaa tttttatttc
     2281 cactattgag ttccgatctg actcttcacc ttcgggaata agcgcgccaa gtcctttacc
     2341 caatcctctc ctgctaatca tttaaatcac atcctcactt aattctatga attcatcggc
     2401 caaatccata tatgattccg cacccttgga tttcatatca tattcaataa ttgacattcc
     2461 atagcttggt gcttccgcaa gccttacatt cctaggaatc attgtggtgt agacctttcc
     2521 cctgaaatac tttttgacct cgtctacaac ctggatcgaa aggtttgtcc tgccgtcgaa
     2581 catgctcagt acaaccccct ctatttccaa atcgggatta aggcttctct tgacaagctt
     2641 tatggtgttc atcagctggc ttacgccttc caaggcataa tattcgcatt gtataggtat
     2701 caatacgctg tctactgcag aaagggcatt tatcgataac aaaccaaggg acgggggaca
     2761 gtcaatgaat acataatcat actcgtcttt tatactttct atgcattccc gaagcttcaa
     2821 ttcccttttg ttcatctcgg tcaattcaat ctccgcacct gccagctgga cacttgaagg
     2881 tatgatgaac agattttccc tgtttgtgtc aataatgcat tcccttgggt cgctgccatt
     2941 tataagtaca tcataaattg tcttttccag cgttgtttta tcaatcccaa ccccgctggt
     3001 ggtattaccc tgggggtcga catcaatcat cagcactttc tttcccctgt ttgccaggca
     3061 tgcacacaaa tttatgaccg ttgtggtttt tcccaccccg cccttctgat taaagacagc
     3121 tattgatttg gacatagcat acacctctca ttgtactatt gtgttctgat attattataa
     3181 agttatatag aaataaatgt acatcctttt cttgaacata aatgcttact tccgagaata
     3241 aacatattgt gaatatacat aaattatcaa agtatatgca tgctcacccg gatcatttag
     3301 tatacagcac atattattcc atatatataa tatatcagat taaaaaaaca aatagaatat
     3361 gaatttgcca aagactctcc cacaagtaca aataaggagg acaaaaatgt taaaatccgt
     3421 actttataca tttttccttg tctttatagc cgagctgggc gacaagacac agcttgcgac
     3481 tatgctgctc tctgcaaaat ccaactcaat aattgcagta ttcataggcg caagctttgc
     3541 attgatatgc tcatctttta tcggggtttt cgccggagct tatataacga agcacgttcc
     3601 tcctcaatat attcagaatg cggcaggggt tttgttcatt gcaatgggtc tgctgatact
     3661 aaaggggaaa atataaaaaa cgcgtctcac gacgcctttt ttatattttc ccctttttat
     3721 acatttttgg gtatttttac tgtaatctca atgaattctc ctttatcaac ctgaccgtat
     3781 tcaaccttgc agcccgccgt cagaaactcc ttgcaggcat ttttcagggt gtttacataa
     3841 atattataat tcattttccc tttgattctt ttcttcttag gctcctctgc cgctgccgcc
     3901 gcttcatcta gcatcttatc gaccagctct tcagcttttt ttacgttgag agactttttt
     3961 ataatgcttt tgagagcttt ctcctgcagt gtatcatccg gaagcttcag cagcgccctt
     4021 gcatgcctct ctgtcagttc attctccagt attgtctttt tgatgctgtc cttgagcttt
     4081 agaagcctca gtttattagc tatggtggat tgctttttgc ccacctttct ggcaagctct
     4141 tcctgagtca tcccgtggtc atttatcaaa ttatgatagc cctctgcttc ttctatgaag
     4201 tttaaatctt ctctctgcag gttctctatc agagcaatta cagcagaatc ctgttcaaca
     4261 acctctatta ttactgccgg tatatgactc tttcccgcaa gctttgatga tctaagcctt
     4321 ctttcccccg agatcagctc atatcctttt tcccctattt ttcttacatt gataggctga
     4381 agaacaccgt attccttaat tgaagaagcc aaatcctcca gggatgatcc gttgaattcc
     4441 tttctaggct gatatgggtt tggagctatt tcgtcaatcg gtatattaat tatttctttc
     4501 ctatcgttca aatctttcat tcctctccat accatatgat ttacttttta tattcgctac
     4561 aaacctttga cttcctgcct gagaaagatt gattttactc gttttcagct gttttttata
     4621 taacaaattt ctggtttttt ctgctatgtt atcggatttg atgccggttt acccggcttt
     4681 ctgggatatt ttgtcggagt tgctgaaacc ttctcaataa ctattatatt gtgcctgata
     4741 tcgctgaatg gcagtttata ctcctttacc tcctttattg ttcctccgag aattttgaca
     4801 gctgatgccg cattaccgat ctcctcttct atttcagggc ctttttgaca aagaaagtat
     4861 cctccgatct tcgcaaaagg aatgcaatat tcacataaaa cgttcattga tgccactgct
     4921 ctggacagga caatatcata gcattccctg taagctttat tgccccctgc ttcctccgcc
     4981 cttccgtgaa cagcggtgat atctcttaaa tcaagcttct taattgcttc gctcaaaaat
     5041 attacccttt tgttaaggga atccagaagt gtcattttga gcccatccat caatatcata
     5101 acgggaatcc cggggaaacc cgcaccggta ccgacatcta ttatgctcat accttctttt
     5161 atatatcctg cagacaggca ggtagccgaa tcaagaaaat gtttgatgaa tatctccttt
     5221 tcctcagtga ttgccgtaag gtttactttt tcattccatt ccagcattat ttccttaaag
     5281 gctacaagct tatccacctt tttatttgcc ggttcaatcc ccagctccgt tagtccgtca
     5341 atcagtattt tcctgttttc cagcctgctc accccctcgg ccccttctaa tctgttcaag
     5401 atataccata agcaccgata catctgccgg agacaccccg gaaatcctgg aggcctggcc
     5461 cagattggca ggattgatac tgtcaagctt ttgccttgct tccagtgaaa gtccctttat
     5521 atctgcgaaa tttatatttt ccggtatttt cctgctttcc aattttttaa attgctctgc
     5581 ctgttgcagc tgtttcttaa tatatccttc gtattttatc attatttcta tttcttccgc
     5641 tgtctctctg tctaactcag gcatttcttc gtcaatctcc ttcagagaac agtattctat
     5701 ctccggcctc ttcaaaagct catacaggct tatgcctcct tttatctccg cgctttctct
     5761 gctcattaag aattccctta tatcctctgt cattgaaata ctcttcttct ttagcctgtc
     5821 tgtttctttt tctatattat tcttcttttc aaggtatctc ttatatctct cctcagatac
     5881 aaggcctatg tcatatccct tctgcgtaag cctgagatct gcattgtcct gccttagaat
     5941 aagcctgtac tcggctcttg aagtcataag ccggtatggt tcatttgttc cttttgtaac
     6001 aagatcatca atcattactc caatataagc ctccgacctg tctaaaataa aaggctcttt
     6061 tcctctgaga cttaaggccg catttattcc cgctattaat ccctgggctg cggcttcttc
     6121 ataaccggag gtgccgttga tctggccggc aaaaaataaa ccggatattt tcttatgctc
     6181 aaggcttaat tgcagctggg taggatcaat gcaatcatat tcaatagcat aagctgttct
     6241 catcatgtgg cagttttcca gtccttttac actgtggaga atatcaagct gcacgtcctc
     6301 cggcaagctg gaggacatcc cctgtacata catctctctg gtattaagcc cttcaggttc
     6361 tataaatatc tgatgctcct ccctgtcagg gaaggatact atcttcgtct ctattgaggg
     6421 acaatagcgg ggacctactc ctacaatgtc tcctgtataa aggggagacc ggtggaggtt
     6481 tttccgtata atatcatgag tccctttgtt tgtatatgta agatagcacg atagttgatc
     6541 gatatctatg tccccggtca taaaggaaaa tgtatttata tccctgtctc cgggctgttc
     6601 agtcatcctt tcgaagtcca ccgtattacc gtcaattctg gccggtgttc ccgttttgaa
     6661 tctcagcagc tttattccca gatcctcaag gctgtcagag agctttgccg ccggaaaaag
     6721 tccgttagga cccccatcat agcttatctc tccaatcact acttttccct taaggtatac
     6781 tcctgtagcc agaatcacag accggcattc aaatattgct ccaagatgtg ttacaacacc
     6841 ttttaccctg tcattttcca caataatcct cgtaatttcc gcctgtttaa tctgaaggtt
     6901 ttcctgattc tccatcactc ttttcattct atcctgatac agctttttat caatctgtgc
     6961 tctcaatgct cttactgcag gccccttcct ggtattcagc attcttatct gaagcattgt
     7021 tctgtctaca tttattccca tttctccgcc caatgcatct atctccctca caagattgcc
     7081 ttttgcaggg cctccaatag aaggattaca cgccagcatt gctatactgt cgagatttat
     7141 tgccgtaacc agggtcttta atcccagcct tgcacatgcc agggcagcct cactgccggc
     7201 atgtcctgcc cctatgacta caacatcata ttttcctgct gtatattcca tatccacacc
     7261 tctatttgcc tatacagaat ttactgaata tctctttaat gatatcttcc tcaacggtat
     7321 cccctgttat cgctccgagg ctgttccatg cttcccttat atcaaccgat atcaagtcaa
     7381 caggaatatt atcctctatt gataatattg ccctttctat attctcaaga gctctgtcca
     7441 gaaggttctt atgcctgata tttgtaacca tgctgttttt gcttatactt agcttcccat
     7501 gatataccag gccctttatt ctttcctcca gcttatctat cccgatccgg ttctttaccg
     7561 acattcttat tatattgtcc tctccgaaaa cgcctcttaa atacgcctca tcaataacaa
     7621 gccccaagtc tgttttattc aatacagcta ttactttttt attttttatt aaatctataa
     7681 tctcattatc ttcctgccga agctcatcgg atgcgtccag caccaaaata atcaggtccg
     7741 aattactgat ggcgcctttt gtcctctcca ctccgatacg ttcaactata tcctcagagt
     7801 ctctgatacc tgccgtatca ataagcctta ccaatactcc ccccatatta aggtattctt
     7861 cgattatatc ccttgttgtt cccggtatct ctgtcactat ggcacggttc tctttcagaa
     7921 gtacgttcag aagagacgac ttcccaacat taggacgccc tataattgcc gtgcttaagc
     7981 catccctgac gattttaccc tcgtcgaagg actctgcaag ctccataacg gaagcctttg
     8041 cttccagaag catattcttt atgttagccg tcgtaatctc ttccacatca tgttcgggga
     8101 aatctatcga agcttcaata tgggccagca tagaaagaag cttttccata atcttcttta
     8161 tctcttttga caactcccct tccagttggt taagtgcggc attcagcccg gtttccgtct
     8221 tcgctgtaat aacatcaata actgcttccg cctgcgccaa atctattctt ccgttaagga
     8281 aagcacgttt tgtaaattcc cccggctcag caatatcggc accgtttctc aggacttctt
     8341 ccattatcct tctgatgggt acaatgcctc catgacagtg cagctcaaca acgtcctcca
     8401 tagtatatga ataaggcttt cgcataacaa tcacaagcac ttcgtcgatt atagcaccct
     8461 tgctgtcaac agcatggccg tatactaccg ttcttgaagg tatatcatct atatccataa
     8521 ctctttttgg cttgaaaact ttttttaata tctccgtgga tttctcaccg cttattctca
     8581 caatccctat gccggcttca cccggagcag tagctattgc agctatagtc ctttcaaaca
     8641 ttatatcctc tccttagagt cagctgtact gtttttatta ttgtttacaa tgttttaaat
     8701 atgtacatat aggttattat atatcactgt ttaaattttg gaaacaaatt tatatgtgag
     8761 acatggggga ccggggacat ggggacgttt cgcgtgtccc gcttccagtg ggacagggga
     8821 aacgtcccca tgtccccatg tccccatgtc tcacatataa aaagggatat cgctagatct
     8881 gcaacacccc gttcatactt tccaataata tttcagttga aattttctat ttatttgata
     8941 taataacttt tctgtacggc tcttcccctt cactaaaagt tgttatacct tcaacttcct
     9001 gaagtgtaga atgtattatt cgtctttcat atggattcat aggctcaagc tctatatttt
     9061 tacctgtctt ccgagccctg tcggccagcc ttttggcaag tctctgaagc gtctcctctc
     9121 ttttccttct ataatcctca gtatcaagta tgacttttac gaagctgtct ctttccttat
     9181 ccttgttaac aaccagactt acaagatatt gaagggaatc aagtgtctgc cctcttctcc
     9241 ctataatcat acccatcttt ggacctgaaa gattgatata taagctttcg tttatattct
     9301 ttactcttat ttcagctttt atatccatgg cttccaatac ttctctcaaa aattttcttg
     9361 ctctttcctt ctccagggta tattcggtta ttcttatttc tttattttct atttcctttt
     9421 taacactttt ctcggtagtt tttttctctt taagagttac tcttactttt gcatttttag
     9481 agccaaggat tccgaatatt ccctttgtcg gctgttccaa tatctctata tccacattat
     9541 ccttttcagt ctctaattcc tctaatgcag attgtacagc ttcttccact gttcttgccg
     9601 ttttttcaac catcatcata tctattattc ctccttttta gcagcctgag gcttcggctt
     9661 catcatgaaa tactgctgta tgatcgtaaa tatattaccg gcaacccaat acaaggcaag
     9721 cccggaagga acattgagag taatccatcc gaacattatg ggcattatat ttgtcatcat
     9781 gctctgtgtc tgagctgctg tatcctcctt gccctttttt ttattggctt taggagcact
     9841 tatcatcttc atctgataat atgttgtcgc tgctgataat acagccagta taggcactcc
     9901 aaatcctgca actttaatca ttgtcgcctg catttccggt ttacccaggt ctaatagcca
     9961 ccaaaaattc ttcgcaatcc cggcgtatat attagcatcc ttaaatacat actttaccgg
    10021 attctgtatg acataaaaca atgcaaaaag aatgggcatt tgaatgagtg ccggcaggca
    10081 tcccgaagca ggattataat tttgctcctt atacagctgc agaagctttt gattcatctt
    10141 ttccttgtcg ttcttgtatt tttcctgaat ttccttcatt ttagggttta gttcattcat
    10201 ttttttagtg gattcagtct gcttgatatt aatagggagc agtaatacct tggttaatat
    10261 tgtaaaaatg attattgagt atccgtaatc tcctacaaag tcaaatatga acttcaacaa
    10321 cgcacctaaa ggttcggcaa taaaatctaa caaatctttt cccccttata tttcagttta
    10381 tagcaagatt tcgaggcaga ggatatatgt aaacccttat tggaattctt ttcgcctcgt
    10441 tataattaaa ctaacggttt ttcttctccg gtacgggatc ataaccgccg ggattaaacg
    10501 gattgcatct taaaattctg cctgcggaca gatataaccc ccgtatgact ccgtgcctgt
    10561 caattgcttc aattgcatag ctcgagcaag taggaatgaa tctgcatttc cctcttgata
    10621 atggagatat aagcaactga tatgttttaa taataactat aaggattttt ctcatttatt
    10681 cctcgctttt tttaagcttg gcccttttca aaattgatat tatattattt tcaatcatcg
    10741 aataatccgc tgacccgctt cctgcccttg ctgtaaaaac aatatcatag cctaatttta
    10801 cagtatccgc aaactttctg tatgcttcct tcatcagcct tttgattcta tttctgatta
    10861 cgctttttcc aaccttcttg cttaccgaga aacccactct attaaaattg gaatcatttg
    10921 gaattatata catgacaaca aatttttcca cataatactt tccttcgtta taaacttttt
    10981 tgaactcata gtttttctta agcttatgca atgttgtcat atcctgcctc ttccccttca
    11041 tacagaaaaa ggccacagtt cgcggcctta tactgtcagt ctttttctac cttttcttct
    11101 tcgccttctg aggacttctc tgcctgtagc tgtgctcatt ctctcccgga agccatgaac
    11161 tttttttctt tgtctttttt ttggctgata tgtcattaac atgttatgca ccccctaatc
    11221 tctgagtata tcttaattca aagcaattag cttaatgtaa taaagcataa ttcaccactt
    11281 tactattaaa attatatagg cattgatagt ttgtgtcaat acaatctttt gctgcagcgg
    11341 acatagctgc accaagtgca aataaagtat tttcagtatc tttgccttta tttaagatat
    11401 tcttatttgt attgaacttg accacctttt ttgctattat tattatatga tgtgtataac
    11461 cctgcatatt tactgaagtt atcaacaact gtgcataatt tgtgtataac ctgtttgtgt
    11521 gtattaactt ctattttatt gtcaataatc tgataactac gtttatttat attaacaact
    11581 tatccagata atattaacaa tatttacagt atatattaac aagttaatca catttttatt
    11641 catacttaaa aaattatacc gcaattttcc atacacttgt ctcgggatat aggagcgaag
    11701 agaattttcg gttaaggagg cattctatgt attctcaatt agttgaaatc tgggaaaaaa
    11761 ctttaagcgt aattaaggtt gaattgacag aagtaagctt taatacatgg ctcaaagcca
    11821 tacaacctgt ttctataaag gaaaatctta ttacattctc tgtgccggat gattttacaa
    11881 aaggcatact tgaagcaaga tacagcaagt tgataaaaaa tgcattaaag caagttactt
    11941 ctgaatatta cgatataagg ttgatcattc ccaccgaaga aaattttaca tcaataatga
    12001 cagaacggtc tgaaataaat caatctgagg aatctgtcaa ttcctccttg aaccctaaat
    12061 atacttttga aacctttgta ataggaaaca gcaaccgtct tgctcatgca gcctctgttg
    12121 ctgtagcaga atcaccggcc aaagcatata atcccctctt tatatatgga ggcgtcggat
    12181 tgggtaaaac ccaccttatg catgcaatag gccactacat acttaaacag aatatgagat
    12241 ccaaggtcgt ctatatttct tcagaaacct ttacaaatga acttatcaat tcaatcagag
    12301 atgacagaaa taatgaattc agaagcaaat acagaaatgt agatgtgctt ttaatagatg
    12361 atatacagtt tatagccgga aaagaaagca cccaggagga attcttccac acctttaatt
    12421 ctttgtatga ggctaacaaa caaataatta tatccagcga cagacctccg aaagaaataa
    12481 gaaccttgga agagcggctc agatcaaggt ttgaacaggg tttgatagcc gatatacaac
    12541 ctccggatct ggaaaccaga atcgcaatat taagaaaaaa agcaaaatct gagaatcttt
    12601 cgatatctga tgacgttatg ctgcttattg caaaaaaaat tcattcaaat ataagagagc
    12661 ttgaaggagc attgataagg gttgtggcat attcttccct tacaaacggt gaaattaatg
    12721 aagaacttgc aaatgaagct ttgaaggata tattatcaaa ctcaaaacca aaaaaggtta
    12781 ctccttcagc gataaaagat gttgtgggaa attattacaa tataaaaatt gaggatttca
    12841 atgcgaaaaa aagaaacaaa tccgtagtat tgccaagaca aattgcaatg ttcctctgca
    12901 gagagcttac agacctttcc ttaccgcgga taggtgaaga attcggcaga gaccatacaa
    12961 caataatgca tgcatacgaa aaaatatcct ccgatataaa aattgacact cagttaaaat
    13021 cagttttgga ggagttgaaa agtaaaatag aaggttaata tcctgttgat atactattaa
    13081 taacctgttt ataagttttt tgattatttt agttatactt tgctatcaac agaattcctt
    13141 cgtaaactcc acagattata aacacgcaca ttatttctta aagcaggatt tttggggctt
    13201 ttgcacttat taacagcccc tactactatt actactaaaa ttattctttt atattatata
    13261 aaaataaata tcggaggtta tccacaatga aaataataac tacaaaatct acattattgg
    13321 aatccataaa tgtagtacaa aaagcagttc cttcaaaaac cactcttcct atattggaag
    13381 gaattctttt tgatgcaaaa agcggcaagc tcaagctgac ggcaacagat ctcgaaatgg
    13441 gaatagaaac aacatcaaac gttgatgtta ttgaaccagg caaaattgtg ctgtcttcaa
    13501 gaatggtagg agaaatagtc agaaaacttc ctgattcgga tgtagaaata gaaacggcag
    13561 aaggaaattt agtcagcatt aaaagcgaag gctcaaaatt caaaattgtt actcttcctt
    13621 atgatgaata tcccgagctt ccggttgtga aaaaggagaa cggaattact attaaacaaa
    13681 acattctgaa ggatatgata agaaagacga tatttgcagt atccactgac gaaatcaggc
    13741 cgatacttac aggagtactt tttgatgtat cgggtgaaga gcttacgatg gtggctctcg
    13801 atggtttcag aatggcggta aagaaaaatg ttattatgaa tgataataat tttaaagccg
    13861 ttattccggg aaagacactg tctgagatag gaaaaataat aaacgataat gaagaaaaca
    13921 taaatattta tttttcaaag aaccacattc aagttcaaat tgaggatact attataatat
    13981 cgagactttt agagggagaa tttataaatt ataaacagat aatacctgca gaatataaaa
    14041 taaaaacaaa ggtgtctaca aacctgctta tggaggcttg cgacagggca gctttatttg
    14101 caagagactc cagcaacaac atgataaaat ttgaaataaa cgatgatatg atggtcataa
    14161 tgtcaaattc gcagaacggt gatgtacacg aggaacttaa aatacaaaaa gaaggaagcg
    14221 acatagaaat tgcatttaat gcaaagtatt tcatagacgt gcttaaagta atagaaagcg
    14281 aagaaataac aatagaattt actacaaacg taagcccaag cataataaga cctgttaagg
    14341 atgaggaata tttatatctg gttcttcctg caaggttcaa aaagaacagc taaattaaaa
    14401 gggctgctgc aaaactatat ttcgcagcag ccctttagtt aaaaaagaag agagtgatat
    14461 tatgagagaa gcaaaaatag aaacagattt tattaagctt gatcagttcc tgaagtttga
    14521 aaatattgca ggaaccggcg gagaagcaaa gaacatgatt atggacggat tagtaaaagt
    14581 taacggaaat atagaaaaag caaggggtaa aaagctccgc aggggagatg ttatagaaat
    14641 ttttggcgaa gaaatcaagc ttatctaata aattataggg gtgcttatat tgtatataaa
    14701 aaggattgat ttgaagaatt tcagaaacta taaagagttt gatattgagc tgggcaaagg
    14761 agtaaatata ttttacggtc agaacgcaca aggaaaaacc aatataattg aatccatata
    14821 tatggcatct accggcaaat ctcaccggac gcaaaaagac atggagctta tcagatggaa
    14881 cagtgaagac gctaaaataa gaatagatta tcaaaaaaaa gaagaagaaa aaatagaaat
    14941 atatttaaat aaaagtctaa agaaacaaat taaaaagaac ggagtaaggc tgtttaaaat
    15001 aggagagtta ataggaaatc ttaatacagt tatattctct ccagatcata tgaaaataat
    15061 aaaggaaggg cctgctgaaa gaaggaggtt tattgacata atactatcac aggtaaaacc
    15121 gggttattat tataatctgg ttcagtatct caggactctt gaacagagaa ataacctgct
    15181 ggaagaagca agaacaaatt caggcattct taagaccata gacatatgga atgaacaact
    15241 agctgatttt ggtgccaggt taataaaagc gaggcaagat ttcatatgtg acatatgctg
    15301 catggcagct gatattcaca aaaaaatatc caacggaaaa gaaacactgg ttatcaaata
    15361 taattcttca ataaagtatc atagtaattc ggaaaaggaa ataaaagaga attttatcat
    15421 tgcgctggaa aaccatatta atgccgatat gggaagaagc gctacacaca aaggacccca
    15481 cagggatgat atattatttt ttatcaatgg aacagaggta aagacatatt catctcaggg
    15541 ccaacagagg acaacccttc tctcattaaa aatagcagaa ctcaaatata tgaaagaaga
    15601 gacgggagaa ttaccggtac ttttgttgga tgacgttttt tcagagcttg atgatgagcg
    15661 gcaaaaatac ttgattgagt ttattaaaga catgcaggca atcattacct gtaccaatgt
    15721 agaacatctt gaaaagctta tgcttgaagg gtctcggaca tataaagtgg aaagcgggat
    15781 gatagtaaaa gattagcttt tagtttttta cagaaaaggt tataataata tggtatgaac
    15841 aagataagtt aattaggatg caagggagga caagttgatg tttatgcatc taggaggaga
    15901 tgttgtaata tctctgaagg atataatttc aatcatggat atagaatcca gcagtacatc
    15961 cagtattaca aaagagtttt taaagactgc agaggatgag ggctttataa gaagaatatc
    16021 agaggatgaa gaaaagtctt ttatactgac ggaaaagaac aaaaagacaa taatatattt
    16081 gtctcccatt tcttctgtaa ccttatttaa aagagcagga ttcataaaag acatatcgct
    16141 gaagtagagg gggaatagga atggaagaaa ataagtttga aggcacatat gaagcggaac
    16201 agattcaagt acttgaagga ctggaagctg ttaaaaaaag acccggaatg tatataggca
    16261 gcaccggatc cagaggcctt catcatttgg tttgggaggt agtggacaac agcattgatg
    16321 aagcattggt aggcgcctgt aaaaatataa aagtaaccat acatcctgat aattcaatca
    16381 gtaatgaaga tgacggaaga ggttttccgg taggaatgca tccaaagctt aacaaacccg
    16441 cagtagaagt agctcttaca gttcttcatg cgggaagtaa attcggagga ggaggctata
    16501 aggtttccgg cggtttgcac ggtgtcggag tatccgtagt caatgcttta tccgaatggc
    16561 ttgaggttga ggttaaaaga gatgggaaga tataccacca gcgatatgaa aggggctttc
    16621 ccgttaccga attaaaagtc atcggtacga caaacagaac aggaacaaga gtgtccttca
    16681 agcctgacag tgatgtattt gatgaactgg aatacagctt tgatatattg gaacacaggc
    16741 ttagagagtt ggcatttctt aacaaaggaa taaagataag cctcaaagat gaaagggacg
    16801 gaacagaaaa gacatttcaa tatgaaggcg gaataatatc ttttgttaag catcttaata
    16861 agaacagaga ggttcttcat aaggagccga tatattttga agcaaaaaaa gacaatgttg
    16921 atgttgaaat agcgcttcag tacaatgacg gttatgcgga gagtattttc agctttgcga
    16981 acaacattaa cacattagag ggcggaagcc atatgagtgg cttcaagaac gctgtaacca
    17041 aagtgataaa tgattatgcc agaaaatata aatttcttaa agaaaatgat gccaatctta
    17101 taggtgaaga tgtaagagaa ggcatgtcat gcgtaatcag catcaggctt accgatcccc
    17161 agtttgaggg acagaccaag acaaagcttg gaaacccgga ggtcagagga atagttgaca
    17221 gtattgttac agagaatttt gaagcctttt tggaggaaac cccttcaaac gcaaaaataa
    17281 ttgttgagaa agcactgact gcagcaaggg ctagggatgc agcgaaaaag gcaagagagc
    17341 tgacaaggag gaaaaatgta ctggaaagca ctacactccc cggtaaattg gcagactgct
    17401 ctgagaggga tcccagcctt tgtgaaatat atatagttga aggagattct gcgggaggtt
    17461 ctgcaaaaca ggggagagac agaaggtatc aggcaatact tccattgaga ggtaaaatac
    17521 ttaacgtaga aaaagcacgg cttgacagaa tattgaacag cgagatgata agagcaatga
    17581 taacagcttt tggggccggt ataagcgatg atttcaattt agaaaaaatg agatacggca
    17641 aaataataat gatgactgat gccgatgtag acggtgcaca cataagaaca ttgctcctga
    17701 catttttcta taggtacatg agtcctttga tagaagccgg atatgtttat attgcacaac
    17761 cgccgcttta caaagtacgg aagaagaata aagactatta tgcatataat gacaaagagc
    17821 ttgaaaaagt gttcaaggag ataggcagag agggttccag catacagagg tacaagggtc
    17881 ttggagagat gaaccctgag cagctgtggg atactacaat gaatcctgaa acaaggatca
    17941 tacttcaggt aacaatggag gatgctgtgg cggccgatga gatatttaca gtactaatgg
    18001 gagataaagt ggagcccaga agagagttta ttgaacataa tgctaagtat gtaaggaatc
    18061 ttgatattta agataagctt tggaggtaat tgatttggat tatgagaatc aaaagatact
    18121 gcccataaat attgaagaag aaatgaaaaa atcattcata gattacgcaa tgagtgttat
    18181 agttcaacgt gcattgcctg atgtaagaga tggtttgaag cctgtccaca gaagaattct
    18241 atatgcaatg aatgacttgg gaattactcc cgataaacct catagaaagt gtgcaatgac
    18301 tgtgggtgaa gttttaggta aataccatcc ccatggtgac tcttctgttt atgatgcaat
    18361 ggttcgaatg gctcaggact tttctcaaag atatatgctc attgacggcc atggaaactt
    18421 cggttccgta gacggagacg gtgctgcggc tatgcgttat acggaagcca gaatgtcaaa
    18481 aatagctatg gaactgctga aggaaataga taaggaaaca gttgatttta caccaaacta
    18541 tgatgaaagc cttaaagaac cgactgttct tccggctcgt tttccgaatt tactggtaaa
    18601 cgggagcaat ggaatagctg ttggtatggc aacctccata cctccgcata atctcggaga
    18661 ggttattgac tgtgcagtat caaccattga aaatcctgac atagaattgg aagagatgct
    18721 taaatatata aaaggccctg attttcctac cggcgctata attatgggca aggaaggaat
    18781 aaaaaaagct tatagtaccg ggagaggaaa ggttatcgta agggctaaag cagaaattga
    18841 ggaaatgagc ggaaacaggc aaagaattat tgtcagtgag ataccctacc aggtcaataa
    18901 agccataatg atagagaaaa ttgcagatct cgttaaagaa aagaaaattg acggtatctc
    18961 agacttaagg gatgaatccg acagaaacgg tatgagggtt gttatagaac tcaagcgaga
    19021 tgcaaatgcc aatataatac tgaatcagct ttataagcat acccaaatgc aggaaacctt
    19081 cagtataatt atgattgccc ttgttaacgg acaaccaaag gttctgaacc ttaagagcgt
    19141 tatcactcat tatatagacc accagaaaga cgttgtaacg agaagaactc aatatgatct
    19201 taaaaaagcc gaagccaggg cacatatatt ggaaggcttg agaatagctc ttgataatat
    19261 tgatgagata gtaagcatac taagaaactc caaaaatgtt gctgaagcca aggaaagaat
    19321 gatgagcaga ttcggccttt cagaaataca ggcggaagca atagccgaaa tgaaacttaa
    19381 gagcttaacc ggcttggaaa gaggcaagat tgaggaagaa tatctttcaa taataaagaa
    19441 tattgaatat ttcagatcac ttcttgctaa tgaacagaag cttttaatgg ttataaaaga
    19501 cgaacttttg gaaataaaga aaaaatttaa tgacaagaga agaacaaaaa ttattcctac
    19561 atcattagag gaatttgatg aagaggattt catagaagag catgaagtag ctgttacaat
    19621 tactcatttc ggatatataa aaagacttcc tgcggacacc tataagagtc agagaagagg
    19681 aggccgcgga ataacaggac ttcagaccag agaagacgat tttgtagaaa atctgtttat
    19741 aacctctaca cataattata ttttgttctt tacaaacaaa ggaaaaatgt ataagcttaa
    19801 gacctatgag attcctgagg caggcagaca ggccaaagga acagctttgg tcaatcttct
    19861 gcagctggat gcagaagaaa aagtaaatgc ggttattcat ataaaggatt ttaatgacgg
    19921 caaatatctt attttcatga caaagcacgg tacaataaaa aagaccgaac tgaaagaata
    19981 tgatacttca agaaatacag ggattacagc tataaacctc attgataaca atgaactaat
    20041 cggggtcaaa ctaacccgtg ggacaacgga gttgatagcg gctacaaaaa aaggacttgc
    20101 aataagattt ccggaaagcg atgtaaggcc tatgggaaga agtgccaggg gagtacgtgg
    20161 aataaactta aatcgcgatg atgttgtgat aggaatggat attgtagagg aaggagccga
    20221 ccttcttgta attagtgaaa aaggttacgg caaacgtact ccgatagatg aatacagagt
    20281 tcagacaagg ggcggcaaag gaataataac atcaagaata acccccaaaa ctggacatgt
    20341 tgtgggaatg aagatcgtga aagagcaaga cgaaataatg ttaatcaatt tatcgggcat
    20401 tattataaga atgaatgtca acgaaatatc aaaaatggga agaagcactc aaggcgttaa
    20461 actgatgcgg gttcctgaga acgaaagcgt agtatcaata gcaaaaataa gcagcaatga
    20521 ggaagaagag aagtaacggc gattctttaa ggacagtcat gcataacctt aacagtttgt
    20581 ataaggttat gcatgatttt ttattgtgag gataaatcct ccacctcatt gaaatcttgt
    20641 aataacacgg aaaaagcaga aaaatatcaa gattcgaact attaataaat aatagtaggg
    20701 taaaaggttt ataatattaa aggaatgtta tctgataagc atagaaaaaa ttagtagttg
    20761 acgcttatca tatatgtccc ttccggacag cgatgctgtc tgtcagcaat gaagcaaaag
    20821 cttcattgaa tggacataat caaggtatgt tatatgataa acttatggaa tgaattactt
    20881 attgacgttt atcatataac gtgataagat aagattcctg cttcggaatc ggcgaagcag
    20941 aaaaagacag gaaaaaagtg aaactcagaa ta
//
LOCUS       DAOOAB010000045        20548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3431_length_20548_cov_11.812521, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000045 DAOOAB010000000
VERSION     DAOOAB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_20548_cov_11.812521"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..596)
                     /locus_tag="PKU88_09520"
     CDS             complement(18..596)
                     /locus_tag="PKU88_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69553.1"
                     /translation="MERDFLSECFEEKEVRLKQLNQINIEIMLLEKLINKHKRQTQKL
                     INMDPDYKRYKEKCNRDEEYSGNLDYDEYCEFYKLLIESPAGSELYSKLLKLLKIQSG
                     ISYSSENEFDKRLWLQIMDVLSTKMPEKEFGIWMREVYPISWGDNIIKLSVNNGMNKY
                     ILENEYEPMLIEAIKDITGNVYRIEYVIGESR"
     gene            complement(1553..2581)
                     /locus_tag="PKU88_09525"
     CDS             complement(1553..2581)
                     /locus_tag="PKU88_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPX69554.1"
                     /translation="MKKYSNIYDLCEKDQNICRHLYTQVNNLYRSEINKGKILEDTGI
                     RYRDALHLLSKFLVECYHKKNLNYIESRHIYAFAEWAWETGYSKRTIFTTLSSTRDFY
                     DRVRKDGSSLYLPTNDMLRMELEEIGFSSINNIEKIGDQRAWTVSEINKMKEKAIELG
                     HEKYADIIDLSWLFGTRIHETIRLNYYQLENAIRMDKLTVKGKGGLVRSISIRNDKQR
                     EIIEKLLCGSIKGKVFVAEGEKAHEIIKKVQHFIYNHRGVVQEKIEAWEVFDQYCIKA
                     GVHKIDGYDISIERGDITAHGLRHTWAIEYYLELIAAGVDDIAAKKIVSKELGHHRIE
                     VTKVYLKI"
     gene            complement(2568..2942)
                     /locus_tag="PKU88_09530"
     CDS             complement(2568..2942)
                     /locus_tag="PKU88_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69555.1"
                     /translation="MNNQLSDEQKKILAKTKRIIDLQFRGYREGKHTSRRTYRSVVHA
                     YARYIITQKGGKEIAINLDDVQSYIDSLIKKGRTVSYANGVICALKYYTRKTHGKDSK
                     VLHQLDRVKVITGEERIEYEKI"
     gene            complement(3536..3748)
                     /locus_tag="PKU88_09535"
     CDS             complement(3536..3748)
                     /locus_tag="PKU88_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX69556.1"
                     /translation="MIKIKLSELMGKHRMTQKKVAKETKIWPSTVSAIYHERVKYIDI
                     NHINELCKLFNCQPGDLFEYIEDENE"
     gene            3985..4581
                     /locus_tag="PKU88_09540"
     CDS             3985..4581
                     /locus_tag="PKU88_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001993219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69557.1"
                     /translation="MSNMLKAKVSITGFRPLLFNHFGPESLPLSKTERKGVAGNNPEE
                     WKKTVLCDDNKQLYLEPSYIFACIRDGARYTKKGRGTLQSAIAATLQVIDDRILLDRY
                     LPNEIEIDPTQPVFLDVRSVRNPTTKSRNIRYRVAASKDWKINFSISWDKTIVNREQI
                     HSILIDAGMYCGLGDGRSIGFGRFVVENFEVIEDKESA"
     gene            4574..4864
                     /locus_tag="PKU88_09545"
     CDS             4574..4864
                     /locus_tag="PKU88_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPX69558.1"
                     /translation="MPKKRPPKEVWYKIREIVWNRDKKSCTHCHTPLSLDKCHIDHII
                     SGKCGTNKLNNLRTLCRRCHVLRSDNRHRGMIAKALKDGIITTDWRKHTWDG"
     gene            5354..5521
                     /locus_tag="PKU88_09550"
     CDS             5354..5521
                     /locus_tag="PKU88_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX69559.1"
                     /translation="MSKTYKVSDIETILQLSRTKAYALVNQGLFPVIRIGKVIRIPAE
                     LFDNWMISQMN"
     gene            5606..6817
                     /locus_tag="PKU88_09555"
     CDS             5606..6817
                     /locus_tag="PKU88_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPX69560.1"
                     /translation="MDGSIQERINTKGKTVYDVIFRVIDSKTGKKKQKLKRGFVKKGE
                     AQKYLTEVLGQINNNSYIDAKKITLGELLTEWFSKYVEGKLAPNTIRGYRVNIYKHLI
                     PNIGWIPLQNITSRDIENFYYELYEDENDDTSTGLSATSIEYIHRNLSKALDYATKER
                     LILRNPARDAERPSKEKFYGDVFDQQTIMQLLAVIKNDPMELPTALSGLSGLRRGEDA
                     GLMWIDVDLDRCILNIRKQRTAGAVSTKRDKLKTLGSYRSIRITQTLSEILKRHKDLQ
                     NKNKEILGEDYVDTGFVCCEPDGSIVDPSTYSKRFSQILKKHNFKQIRFHDLRHSFAT
                     NLIRLGVPINTVSKMLGHSSVTITLDIYAHVLEEMQQDAVLKMDEDISRYIDNQDDII
                     DINTNNKTDER"
     gene            complement(6976..7066)
                     /locus_tag="PKU88_09560"
     tRNA            complement(6976..7066)
                     /locus_tag="PKU88_09560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7029..7031),aa:Ser,seq:gga)
     gene            7248..8432
                     /locus_tag="PKU88_09565"
     CDS             7248..8432
                     /locus_tag="PKU88_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPX69561.1"
                     /translation="MKLKIDRTLLKYVYFVVLATILYVIYRLISSFEYILGALIGALS
                     GLLAVLAPFMIALVVAYLLHPLVCWIESNVMYNKRITKAEADSSDKHKKLKRTMSVLI
                     TYLLVLGLIVVLLYSSYAMIGGKFSSQVDINSVIDSLVSYSERYIHIFGQLRSWLENS
                     GLSDNLKQQLINTVESANELISSAISNSFIKIKSFGSNIVNIVLGLIIAFYILKDLEY
                     FRELYGKTIKALIKEHKNEKLSNLFEDINSILSHFIRGQLLAALIVGILSSIGLSIIR
                     LDFAILIGMTAGIANIIPYFGPILGSVPAVIVGLLSGNPVKALLAIIVLVAVQQIDGT
                     LISPRIVGSSVGLHPVFVMLAMIIGGSYLGLLGMLIAVPIAAIIKMFLVRWIENKKNI
                     EA"
     gene            complement(8438..9637)
                     /locus_tag="PKU88_09570"
     CDS             complement(8438..9637)
                     /locus_tag="PKU88_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPX69562.1"
                     /translation="MYQSDATGQNTLYVNDLLVRIFKLMLVAIAAFVILKAAGIILIP
                     WLHTIVIAGTVIIACAVPIACRGFSMDVNTVKSVNIYCMAVICILGYSYLNMGIIMLL
                     AIPIGFACMYFDLKLISRSIGISVLGIMIGELLRVGSNWSFDAAVQNSYIRITLYFFQ
                     FGVAAALITAISKRVLSILSNTHSHYENINNIFSNIQVSSQSLEAAEQTLLNGVNGLS
                     VNNEEDTASSNTVVKGIISNINKSMENAREIMKYTQTMLKGKAKDLKAGEEAARIEEY
                     ARNSKEIILKLDKYTEKIKENLSLISVMIDESKLLSMNASTEAENASSGGRGSAIIAM
                     KVEKLADESVESANHIQELLNNVVNDAENTVKSVAEAYEEMFKSLELINRTVETLIKW
                     LMYKNMK"
     gene            complement(9937..12264)
                     /locus_tag="PKU88_09575"
     CDS             complement(9937..12264)
                     /locus_tag="PKU88_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPX69563.1"
                     /translation="MKIERKAVRICLVCILAVCIALPVLTFAREDTFIELPERIGTSP
                     EIVLEIPFDAPLMASTVSTGNVYVMDDQGSKVPLSRKMGSEWKSIIIEPLAQYEAGKA
                     YTLYIRNAVKYATGSRLKEGFKMKFTIAKAAAEPLPTVGSRENLRKLLEEAAEQDYYM
                     DYGIYKRNGLTAVDTAAAAPASAAAESAGSSNKAKQAADEGSGDYSTTNVQVEGVDEA
                     DIVKTDGKYLYQVNNNRIVVAEIYPSDKMKITKIISLEEENVYPTELYLDNKYLILIG
                     ASNNSIPVYRPGQSSIMPEYYPHYSNETVKLMAYDITDKSNIKKVREIEMEGSYLSSR
                     KIGSKLYLVSNKRFNYHRILDNAEVNDTPSYRDTAVKEEFINIDHGKIEYFPGSVEPN
                     YMIVAGVNFDLPKEGVNVSTYLGSGESIYASAENLYVAVTRYNNTRVPSPKIYDSAAP
                     KKIEIKVNDRETVIYRFALNNGKLDYTGKGSVPGSILNQFSMDEDKGYFRIATTKGNL
                     FGEGENISKNNMYVLDSDMNTCGSLEDIAPGERIYSVRFMGDRAYMVTFKKVDPLFVI
                     DLKDPQNPAILGALKIPGYSDYLHPYDENHIIGFGKDTIELSNEGIRNREGTTAYYQG
                     MKIALFDVSDVSNPKEKFKEIIGDRGTDSELLNNHKALLFSKKKELMAFPVTVMEIES
                     GGNVEENRPVYGSFSFQGAYVYNIDLEEGFKLRARISHISEEEYKKAGDRWYRGNMNV
                     ERIIYIGDDLYTISKGMIKANSMTDMKEKGSLLIP"
     gene            complement(12307..13275)
                     /locus_tag="PKU88_09580"
     CDS             complement(12307..13275)
                     /locus_tag="PKU88_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPX69564.1"
                     /translation="MKLIDFHCDTILSCIDSDGEVKLRNNDLCIDIRKLKKADSLAQF
                     FAMYVDLDKYSAPMDRCLDMIDCFYNEIEENSADIAFAGSMKELKKNRAEGKISAFIT
                     AEEGGVLEGKLRNLRILYRLGVRLITLTWNYPNCIGYPNFKWEYKDKGLTAFGEEVVA
                     EMNRLGMIIDVSHLSDQGFYDVVRLSQKPFVASHSNARAVENHCRNLTDDMLKKLAEK
                     GGVAALNLEPEFLGDGGSLEAMTRHIVHMTNTAGIEAVAIGTDFDGCSTLTAIKNTGD
                     MHLLSDALSKAGFSGGDIERIMYRNGERVIKDVLRDNWEPYPKSFV"
     gene            13418..13885
                     /locus_tag="PKU88_09585"
     CDS             13418..13885
                     /locus_tag="PKU88_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPX69565.1"
                     /translation="MFREMRRQDRKTDNEKAAAILAAGEYGILSTVGDNGYAYGVPLS
                     YAYSKGSIYFHSATVGHKLDNIKHNDKVSFCVVGATEVIPEDFGTKYESVIVFGKASE
                     IYDEEKQEALMTIIKKYSSDFIENGKKYVAGSVSKAKVIKIEIEHMTGKAKND"
     gene            14032..15447
                     /locus_tag="PKU88_09590"
     CDS             14032..15447
                     /locus_tag="PKU88_09590"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPX69566.1"
                     /translation="MAFEYELVYNNERFYTCLVLLFSIILTSVIITYTLFRVRSKEQR
                     AGSLFLNVIILVYQTSQFLVVVSPPRELYFFVSLQCIGFCILGPAFYLYCRTLLKMGA
                     ISLPETIALYSVSCLGILTVLTNGFHEMFFRIYDMFYIDYGIMYFMALISNSLWAMAG
                     SSLLMKYRVTHEEYNIQQRFLYPICLLAVIGAGAFDGINPELFSYGLTPSSISIIQIL
                     FLFSNRLLGLSGNLFIGKSNILDCINESVIMTDTEGNIIFYNDTQLNRQIGMENGADI
                     QEILDKLTHKYEYSDGLDTLYDYLNLYKTQHNTHKDTAAGTIPNSICGEITIESSGKR
                     WYFYTVQPITKGKIHVAGVLYVFRDVTEDKVLINHLNDRNSKLADTYEKMKKYSAIIR
                     QLSVERERNKILKKISRDIEESVLKIISLLNEIEAMDDKDARSVEISLHKSIAIARNG
                     IAGIRKSISAISEPIMQEGEK"
     gene            15448..16083
                     /locus_tag="PKU88_09595"
     CDS             15448..16083
                     /locus_tag="PKU88_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX69567.1"
                     /translation="MIRVIIADDHSIFAESLQLMLQQDNDIAVEGIALNGKDAVTLCM
                     DLLPDLVLLDIKMPGYNGIEAAKIIKNTFPDIKIAILTTFEDFENITQAFINGINGYI
                     LKDITSKELVLAVKCIICGFQVMHGPAGEFLQREFIRLSNNMGYQQQQLKDEDIKIIK
                     LISDGKSNREIGELMNYTEGTIKNRVSRLIELFEVKDRTQVVVHALKNNLI"
     gene            16094..17788
                     /locus_tag="PKU88_09600"
     CDS             16094..17788
                     /locus_tag="PKU88_09600"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPX69568.1"
                     /translation="MTFDIEWLYDIYILEIWHILSIIFSVIFVLSIFLNARKSPVLYY
                     YVVLHSILLEWMICKVLKTIAPTVELKWFFIVAQYFAVCFLGSALLMFGYYYNKRRHL
                     PLWAGILLNIPPVLFFIGIVTNESHHLFYSTYDFLGDTFGPLFYASAGVMYLYLLIGI
                     YFCATCFRKQPGNKNTQAKLLTAGIMVPLLVNALYIFGLVEPRFDFTPISCNISLLFF
                     AYAIYKYQFLDVINIGISLSLGSMQEGVLVTNKRGQIIDYNNSIKEILCFSTNEIPSG
                     TIDRLYDSFELIDNDSILKTCTYDGKIHYIKFYSMPLISKEKEAIGNVHVFTDITAYK
                     ELVFSLKKKNREQVEANKQIIKYINDVEQLTAIEERNRMSKEIHDILGHSLTLVLAIL
                     ESSLILVNENISEAKKRIIQALSAAKQGHSEMKDVLDGKADLSIKSTRRLEMDLENLI
                     DSYKVAGMEVYLSISGTHLNINQQCYNTIFRICQEGLTNAIRHGNAKKADIFIRFGRK
                     EAEVFVIDNGQGCHGINKGQGLSGMEQRIADLKGTLSYGSSENTGFTIHAFIPLQA"
     gene            17968..18462
                     /locus_tag="PKU88_09605"
     CDS             17968..18462
                     /locus_tag="PKU88_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69569.1"
                     /translation="MKVKMLLLLLALLCLVGCKGVKTEEKQNTDIEPIEKVDVREEKD
                     KVVIQAEQGKMEISSDAGQDLDLPEGFPAEKLPVYPKGSIVVSECVNNVYNVGIVTND
                     NVESIYKYYKKNLNADSITSDMLSGESANLFLQDGEKVGAIMIMPNNLPYDFKYVIFI
                     TYGE"
     gene            18480..20435
                     /locus_tag="PKU88_09610"
     CDS             18480..20435
                     /locus_tag="PKU88_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69570.1"
                     /translation="MNLKIFYRIIPIILITLILSFSSAVSASAIPTLPGGAADIGPII
                     PDIPDVPILFLAPSDLSVGAAGETRVNLIWKDNSIDEEGFRIFRREAGGAPYLNIAAT
                     GPHLSETMGYVDNSVERGKTYFYKVNAYKTGTTSAYSNEVTVDVPVLACPNGLSVINY
                     TSRSITIAWNDNSGFESGYNINIGREGSMLFNRYTVPADSTSYLFESLTPGVLYWVSV
                     EVFDEEDNKCETEKITMRTPFEVLQPLNAPTDVNANTVTQNKILLSWRDNSDGETGYT
                     VERKEAGDTDYVILANLNTNSEVYNDNTVIRDKIYMYRVKVLKDMQYECSEPFTVNTF
                     IPEAPSGLNGVEREDSSYRLTWMNDTDYETAYRVERKTYDGTYSLLGYSGPDTLIYDD
                     HTARPDTVYCYRVCAVNGQLVSGYSNECCLGMQAPPHISPEDSEIPAETPVEDYHENS
                     GGTSVDSEGTSRDSEDSADTGIFDGTQSQWAASELEKAFDHDLTYPGIMNNYKNQITR
                     EEFCKIAVKLYEQLSGAIALAGDDPFNDTDDPEILKAYKLQIVMGIGNGQFAPHNKIT
                     RQEICVMIMRALDAAIPEVDRSTASVFPFADKNNIAPWAMDSVKFCYKNNIMKGISET
                     EIAPLADITREQAIVLLKRTFEQYMSR"
BASE COUNT         6165 a         4107 c         3804 g         6472 t
ORIGIN      
        1 tgcaacatca tattcgctta tctgctttca ccaataacat attcaattct atagacattt
       61 cccgtaatat ctttaatggc ctctattaac attggctcat attcattctc taatatatat
      121 ttgttcatcc cattattaac tgatagcttt ataatattat ctccccatga gatagggtat
      181 acctctctca tccatatacc aaattctttt tccggcattt tcgttgatag tacatccata
      241 atctgcagcc acagcctttt atcaaattca ttctctgaag agtatgatat gccgctctga
      301 atcttcagca atttcagtag tttactatat aattcactcc ctgccggaga ctctatcaac
      361 aacttataaa attcgcagta ttcatcataa tcaaggttac ccgaatattc ttcgtcccta
      421 ttgcacttct ctttatatct cttataatcc ggatccatat ttatcagctt ctgagtttgt
      481 cttttatgct tattaatcag cttctccaac agcattattt ctatgtttat ctgatttaat
      541 tgctttaatc gtacttcttt ttcctcaaaa cattctgaca agaaatctct ctccatacaa
      601 aatcacccct tctgattact gcaataatca ttcatattga aacttccgta atgcttatgc
      661 gctctgtgtg attccccccg gaacgctatc tatatccata atctttgtta agctatttta
      721 gcaaagctaa aagcaatatt caatagatta agttatcatt gtaaatcaca tatatgtgtt
      781 cctccactta actctctaat cttcatctat ctattctaga gtaatagaaa tataaaagcc
      841 ttatcttttt ttgtattata ataaataaaa agttggtcag aatgaccaac caaataatgg
      901 taatcgcact cgccttgatt attattctct gtatggctcc ttttgatgtt actctcccgc
      961 attattataa agccatatct attttggggg ctactctttg ctgtttggat acccctgcca
     1021 gaagcttagc cagtcagaat tacattatta ctattagggt atttgttctg cattggttct
     1081 atttcttatt aatgccaacc ttaaatttaa gaaatcttcc tttataattt atgtctttat
     1141 tcttttttgc agcataatat gttaccgcgg taatcggtaa cacaataagt gtaattaaca
     1201 ctataacata atacatgtac atccctcctt tctttgttca tacttgctta acaagaaccc
     1261 tcagcaaaaa aataagcaaa tagaaggttc aacaaaaaaa ttgtcttacc ttatatctgc
     1321 taaccttttg ccacgcgggg caacctttgc taaccttttg ccgcaccggg caacattaat
     1381 aactaacact atcataacaa aggttattat tatatgcaat acttgtatag tcactatttt
     1441 acatattttc accctaataa aaagtgttgt attgggtaaa aaatgcgata atgtgagtca
     1501 tcgatactgt cacattatcg ctcctgaact tgttattatt ctttttaata ttttatattt
     1561 ttaggtagac cttagttacc tcaatcctat gatgtcctaa ctccttgctt acaatttttt
     1621 tggctgcaat gtcatccacc cctgcggcaa tcaactctag ataatactca atcgcccagg
     1681 tatgtctaag tccatgagct gttatgtctc cacgctctat tgatatatca taaccgtcaa
     1741 tcttatgaac tcctgcctta atacaatatt ggtcaaacac ctcccaagcc tctatttttt
     1801 cctgcactac tcctctatga ttgtatataa aatgctgcac tttttttatt atttcatgag
     1861 ctttttcgcc ttcagctaca aacacttttc ctttaatgct tccgcataat agtttttcta
     1921 taatttctct ttgcttatcg tttctgatac ttatactcct aacgagtccc cctttgcctt
     1981 tcactgtcag cttgtccatt ctaattgcat tctcaagttg ataataattt aatcgtattg
     2041 tttcgtggat ccgggttccg aataaccacg agagatcaat gatatcagca tatttctcat
     2101 ggcctagttc aattgctttt tctttcatct tattaatttc tgatacagtc catgccctct
     2161 gatcacctat cttctctata ttgtttattg aagaaaatcc gatttcttct agctccatcc
     2221 tgagcatatc atttgttggc aaatatagtg aagagccgtc ttttctcacc ctatcataaa
     2281 agtctcttgt agagcttaat gtagtaaata tggttctttt gctgtatcca gtctcccaag
     2341 cccattctgc aaaagcatat atatgtcttg actctatata gttgagattt tttttatggt
     2401 aacattctac taaaaatttc gaaagcaaat gtaatgcatc tctatatcta atccccgtat
     2461 cttcaagaat tttacctttg tttatttcag acctgtataa gttgttcacc tgagtatata
     2521 agtgccggca tatgttttga tctttttcgc acaggtcata tatattgcta tattttttca
     2581 tattctatcc tctcctcacc agttattacc tttactctat ccagttgatg caatactttg
     2641 ctgtcctttc cgtgtgtctt ccttgtataa tacttaagcg cacaaattac tccatttgca
     2701 taactcactg ttctgccttt tttgattagg ctatctatgt acgactgcac gtcatctaga
     2761 ttaatggcta tttcctttcc ccccttttgt gtaatgatat accttgcata tgcatgtaca
     2821 acgctccggt atgtgcgccg acttgtatgt ttcccttctc tgtatccccg aaattgtaaa
     2881 tcgattatcc ttttagtttt tgcaagtatc tttttttgtt catcgctcag ttgattattc
     2941 attgtatgct cctcccattt tttgtatatt aattaaagct ttcagcaatt cagatttttt
     3001 tattaaaaat tctttatcac tgtttgcctt aatcccttat aattactatg ctgtagatgc
     3061 cactgatgta ataaaagtaa caccatctca gttatttagt ttagaaagta caactataga
     3121 aataaaagta agcactatgc ccatcctccc aggattagag gtgcttaacg gcgagggctc
     3181 tcttcgtcca tcggacgtgc cacctggcac agagatctga tgatctgtct taccatagct
     3241 gtcaaagttc aactatacaa aaacacccat aagtcgctgc atatgggtgt ttttgtatct
     3301 tttttagatg caagtgttct gcatctttct ttatttactt ttttttaatt tgctggctag
     3361 caaaaaaata aggtcagata atatatatta tccaacctct tgtattctta cctcttaacg
     3421 gcattaccgt taacatgcta accttatgct ttattataaa tacaattttc gcaatgtcaa
     3481 ctggttttca ttaaatatgt actacgtgat acataaagaa caaaacttaa catttctact
     3541 cattttcatc ctcaatgtat tcaaataaat cccccggctg gcagttgaat aatttgcaca
     3601 gctcattaat gtgattaata tctatgtact ttaccctttc gtgatatata gccgaaaccg
     3661 tgctgggcca aatcttagtt tcctttgcca ctttcttctg agtcattcta tgtttaccca
     3721 ttaactccga tagctttatc tttatcatta gatcaactcc cgtttcactt ataaatggat
     3781 ataaactcat cagcccgata ggttaattat attgttaccc taggtaaatt tcaaggcttt
     3841 attacgatgt ttccaaagta attctaaata aaattcgttt tatacgaatt ttgtatattt
     3901 atatgttgac aaatttataa tgttagtata taattcatat tatacgaatt ttattcgtat
     3961 aatatgaatt tgaggtgatg tataatgagt aatatgctta aagcaaaagt ttctattact
     4021 ggtttcagac cattgctatt caaccacttc ggaccggaat ccctgccatt atccaaaacc
     4081 gaaaggaaag gggttgcagg aaacaatccc gaggaatgga aaaaaaccgt actatgtgat
     4141 gataacaaac aactatacct tgagccatct tacatatttg cttgcatacg ggacggggca
     4201 agatacacaa aaaaaggcag aggcaccctt caatcagcaa tagcagccac cctacaggta
     4261 attgatgaca gaatcctatt agatagatat ttacccaacg aaattgaaat tgatccgaca
     4321 caaccggtat tcttggatgt aagaagtgtt cgaaacccta ccacaaaatc aagaaatatc
     4381 agatatagag ttgctgcttc aaaggattgg aaaataaact ttagtatttc ctgggacaaa
     4441 actattgtta atagggaaca gattcattct atacttattg atgccgggat gtattgtggc
     4501 cttggtgatg gtcgctctat aggatttggg aggtttgtag tggaaaattt tgaagttatt
     4561 gaggataagg aaagtgccta aaaagagacc tcctaaggaa gtatggtata agataaggga
     4621 gatagtttgg aatcgcgata aaaaaagctg tacccattgc catactccct tatctttgga
     4681 taagtgtcac atagatcata ttatatccgg taaatgtgga acaaataaac taaacaattt
     4741 aagaacctta tgcaggcgat gccatgtgct tagatccgat aacaggcata ggggcatgat
     4801 tgccaaggct cttaaggatg gaatcataac gactgattgg aggaagcata cctgggatgg
     4861 ttagatattt cgaaatggca ttataaatgc catatatgga gattgaccga ctccatactg
     4921 atgagatttg gttaatttgg acaggtacgg tttgggtatg tatggtctgg tacggtttga
     4981 ttaggtttgg tctggtacgg tttgggtagg ctagtttatg catggtatgg aatggcaggg
     5041 caagggtata ttgagtaagg tttagtatag taatgttcat taaatcttac tcaatattac
     5101 aaaataataa aatataaaaa aggtattgca tttttttaag tattgtgtta acttaaatac
     5161 aagctagtat actagctgtg tttgataaaa taggttggca tataaaaggt tggcaaattg
     5221 caatgcaatt tgcttttttg atttttaatc aaaaataaaa aattctaaaa gccgcaatgc
     5281 aataaaatgc tctgcggctt ttttgtttga gtgacgctag aagcaaatat tagttaggaa
     5341 agggggtttt gtaatgagta aaacatacaa agtgagtgat attgagacta tcctacagct
     5401 ttcaaggact aaggcttatg cactggttaa ccaaggttta tttcctgtta tccgcatcgg
     5461 taaggttata agaatacccg cagagctttt tgacaactgg atgatatctc agatgaatta
     5521 aaagccaaag attaacaaaa atgacaatga taaagatgac tataagataa caaattttga
     5581 tgttaataca ctaggaggag ataaaatgga tggcagtatc caagaacgaa taaacacaaa
     5641 gggtaaaacg gtctatgatg taatatttag ggtaatagac tcaaaaaccg gcaaaaaaaa
     5701 gcaaaaactt aaaagaggat ttgtaaaaaa aggtgaagcc caaaaatatt taactgaagt
     5761 actcggacaa ataaataata atagctacat tgatgcgaaa aaaatcactc ttggagaact
     5821 actcacagag tggttctcca aatatgtcga aggtaaactt gctccaaata ctattagagg
     5881 ctatagggtt aatatctata agcatttaat tcctaacatc ggttggattc cacttcagaa
     5941 tattacatca agagacatag aaaactttta ttatgaactt tatgaagatg aaaacgatga
     6001 tactagtacc ggactatcgg caaccagcat agaatatatc catcgcaacc taagtaaagc
     6061 ccttgactat gctacaaaag aacgcctaat cctaagaaac cctgctagag atgcagaacg
     6121 gccatcaaaa gaaaagtttt atggcgatgt ctttgatcag cagactataa tgcagttact
     6181 tgctgttata aaaaatgatc ctatggaact tcctaccgca ctctccggtt tatccggatt
     6241 gagaagaggt gaggatgccg gccttatgtg gatagatgtt gacttagaca gatgtatcct
     6301 caatattcgc aaacaaagaa ctgctggagc agtaagtact aaacgtgata aactaaaaac
     6361 attaggcagt tatagaagta tccgaatcac tcaaactcta tcagaaatat taaaaagaca
     6421 caaagatctt caaaacaaaa acaaggaaat attaggagag gattatgtgg acactggatt
     6481 tgtatgttgt gagccggatg gtagtattgt tgacccttct acctatagca aaaggttctc
     6541 gcaaatactt aaaaaacaca attttaagca aatacgtttc cacgacttgc gtcattcctt
     6601 tgcaactaac ttgattcgct taggagttcc aatcaacact gtatcaaaga tgcttggtca
     6661 ttcatcggta accatcaccc ttgatatata cgcccacgtt ctcgaagaaa tgcagcagga
     6721 tgctgtacta aaaatggatg aggatatatc aaggtatatt gacaatcaag atgatattat
     6781 cgatataaac accaataaca aaactgatga aagatagaac tctattgaaa aatatcttaa
     6841 caacagtacc acagaattct atagcatatc accaagtatc atgcaatttt gttatcaaaa
     6901 tgttatcaaa cttccattat ataacaaaaa gagggtttca tcgctcctga tgaaaccctc
     6961 ttaaatccag taatatggcg gagagagtgg gattcgaacc cacggaccgc gttaacagtc
     7021 acacgatttc cagtcgtgcg ccttcgacca actcagccat ctctccataa acagagctgt
     7081 tgctcgtact ttagtattat actaaattgg actttaaata tcaacagtat tttgtttgct
     7141 attttacata atttttttgc tgccgaaatg tatgcaatgc tatgaaaatg gcaattttta
     7201 tgatagagta ttcatatact attatgctta agtgtgagga agaacaaatg aagctgaaaa
     7261 tcgacagaac ccttttaaaa tacgtatatt tcgttgtgct tgctactatc ctgtatgtga
     7321 tatacaggct tatttccagc tttgaatata ttttgggcgc gctaatcggc gctttatcag
     7381 gattactggc cgttctggct ccctttatga ttgcgctggt ggttgcatat ctgcttcatc
     7441 cgttagtatg ctggatagaa tccaatgtta tgtacaataa aagaataaca aaggcagaag
     7501 ctgatagttc ggacaagcac aaaaaattaa agcgcacaat gagtgtactt attacatatc
     7561 ttctggttct gggtttgatt gtcgttcttc tttacagttc ctatgcaatg atcggcggaa
     7621 aattcagcag ccaggtggat attaattcgg taattgattc tttggtaagc tattcggaaa
     7681 gatacatcca cattttcggt caattgagat cctggcttga aaacagcggt ttgtcggaca
     7741 acctgaagca gcagcttatc aataccgtag agagtgccaa tgaattgata agctcggcta
     7801 tatccaattc cttcatcaaa atcaaaagct tcggaagcaa tattgtaaat atcgtcctgg
     7861 gacttattat tgccttttat attctgaagg atctcgaata cttcagagag ctgtacggaa
     7921 aaaccataaa agcattgata aaagagcata aaaatgaaaa actgagcaat ttgtttgaag
     7981 atattaattc tatactgtct catttcataa gggggcagct gctggcggca ttgattgtcg
     8041 gcatcctgag ttccatagga ttgtccatta taaggcttga ctttgcaatt ctcataggta
     8101 tgactgcagg tatagcgaat atcattcctt actttggccc tatattaggc tctgtacctg
     8161 cggtaatcgt agggcttctc agcgggaacc ccgttaaggc gcttcttgca attatcgtgc
     8221 ttgttgcagt gcagcagata gacggcacgc tgatttctcc gaggattgta ggcagcagtg
     8281 tggggttgca tcctgtgttt gtgatgcttg ccatgataat cggaggctca taccttggac
     8341 tgttgggaat gcttattgcc gttcccattg ccgcaattat caagatgttc ctggtccggt
     8401 ggatagaaaa taaaaaaaat atagaggcat agcatgccta tttcatattt ttgtacataa
     8461 gccattttat caaagtctcc actgtcctat tgataagctc caggctttta aacatttcct
     8521 cataagcctc cgccactgat tttaccgtat tttccgcatc attcaccaca ttgttcaata
     8581 actcctgtat gtgattagcc gactctaccg attcgtcagc cagcttttcc accttcatgg
     8641 caattattgc agaacccctg cctcccgagc ttgcattttc cgcttcagtt gaggcattca
     8701 ttgaaagcag cttgctttca tctatcatta ccgatatcag gctcaggttt tctttaattt
     8761 tttctgtgta tttatcaagc ttcagtataa tttccttgga attcctggca tattcctcga
     8821 tccttgcagc ttcttctccc gccttcaagt ctttagcctt gcccttaagc atggtctggg
     8881 tatatttcat tatttccctg gcattctcca tggatttgtt tatatttgat attattccct
     8941 ttacaactgt attggaggat gcagtatcct cttcattatt tacgctcagg ccgtttaccc
     9001 cgttcagtaa tgtttgctct gcagcctcaa ggctttgtga ggaaacctgg atattggaga
     9061 aaatattatt gatgttttca tagtggctgt gggtgtttga aaggatactc agtactcttt
     9121 ttgaaatagc cgtaattaat gcagctgcga caccgaattg aaaaaaatat aatgttattc
     9181 tgatataact gttttgaact gcagcatcaa aactccaatt tgaacctact ctcagcagct
     9241 ctcctatcat gatacccaat acggatatgc ctatggaacg gcttataagc ttcaagtcaa
     9301 aatacatgca ggcaaaacct attggtatag ccagaagcat gataatcccc atgttaagat
     9361 aactgtatcc aaggatacaa atcactgcca tacagtagat gttcacactc ttgactgtat
     9421 taacatccat gctgaagcct ctgcatgcga taggaactgc gcaggcaatt attacggttc
     9481 cggcaattac tattgtgtga agccacggaa tcaatattat tcccgcagct ttaagaataa
     9541 caaaagccgc aattgctacc agcatcagct tgaatatcct gacaagcaaa tcgtttacat
     9601 agagtgtatt ctgaccggtt gcatcactct ggtacatcaa agcatccccc ccaaataaaa
     9661 tatggaatat attttaatcg gcatatttct gcataataat ttatttcccg ggaaatattc
     9721 ttattttttg cgcaggcttt accatatatc agacatgtgt atacttatat attaataatt
     9781 cgctgttttt cacccaatat cctgttatta gaaaaaaaaa ccggaaccta agctccggtg
     9841 aaattgctga tgaagagaca cggggagaca cggggacgtt tctcctgtcc cactgaagtg
     9901 agacacgcga aacgtcccca tgtctccatc agccttctac ggtatcagaa gcgatccttt
     9961 ttccttcata tccgtcatgc tgttggcctt tatcatgcct ttggatatgg tatataaatc
    10021 atccccgatg tatattattc tttctacgtt catattgccc ctgtaccagc ggtctccggc
    10081 cttcttgtat tcttcttcgc tgatatgtga aatccttgcc ctgagcttga agccttcctc
    10141 aaggtcaata ttgtaaacat aggctccctg gaagctgaag cttccgtaca caggcctgtt
    10201 ctcctccaca ttccccccgc tctctatttc catcactgtt acgggaaatg ccataagctc
    10261 ctttttcttt gagaagagca gtgccttgtg attgttcaaa agctccgagt ctgtccctct
    10321 gtcccctatg atttccttga atttttcttt gggattgctg acatcgctta catcaaacaa
    10381 tgctatcttc atgccctgat agtatgctgt agtaccctcc ctgttccgga tgccttcatt
    10441 ggagagttct atcgtgtcct ttccgaagcc tattatgtgg ttctcgtcgt agggatgcag
    10501 gtaatcactg tatccgggta ttttcaaggc tccgagtatg gccggatttt ggggatcctt
    10561 aagatcgatt acaaacaggg ggtcaacctt tttgaatgtc accatatatg ccctgtctcc
    10621 cataaatctg accgagtata ttctctcccc gggagctatg tcttcaaggc ttccgcaagt
    10681 attcatatcc gagtcaagga catacatatt gttttttgat atattttcac cctcgccaaa
    10741 cagattcccc ttggtagtgg caatccggaa atatccttta tcctcatcca ttgagaactg
    10801 gttcagtatc gagcccggta cagagccttt gccggtataa tccagcttgc cgttattcag
    10861 tgcgaatctg tatatgactg tttccctgtc atttaccttt atctcaatct ttttcggcgc
    10921 cgcagagtcg taaattttgg gactgggcac ccttgtattg ttatatctgg taacagccac
    10981 atagagattc tcagccgacg catatatact ctccccggaa cccagatatg tggatacgtt
    11041 aacgccttcc ttggggagat caaagttgac accggcaaca atcatatagt tcggttccac
    11101 cgaccccggg aaatattcta ttttcccatg gtctatattt atgaattctt cctttacggc
    11161 agtatccctg taggaaggag tgtcattaac ctctgcattg tccaatattc tgtgatagtt
    11221 aaaacgtttg ttggatacaa ggtacagctt tgagcctatt ttccttgaag acagatagct
    11281 gccctccatc tctatttctc ttactttctt tatatttgac ttatcggtaa tatcataagc
    11341 catgagtttt actgtttcat tgctgtaatg gggatagtac tccggcatta tgctgctttg
    11401 cccgggcctg tataccggaa ttgagttgtt ggaggccccg atcagtataa gatacttgtt
    11461 atcaagatag agttcagtgg gatatacgtt ttcttcctca aggcttatta tctttgttat
    11521 cttcatttta tccgaggggt atatttctgc cacgactatc ctgttgttat tcacctggta
    11581 caggtatttt ccgtctgtct ttactatatc cgcttcatct actccctcca cctgcacgtt
    11641 agtggtggaa tagtcccccg agccctcgtc tgccgcctgc tttgccttat tggagctgcc
    11701 ggcgctctcg gctgccgctg aggcaggtgc tgccgccgcg gtgtccacag ccgttaaccc
    11761 gtttctttta taaatgccgt aatccatata atagtcctgc tcagctgctt cctccagtag
    11821 cttcctgagg ttctccctgc ttcccaccgt cggcagcggc tccgctgcag cctttgcaat
    11881 tgtgaatttc attttgaagc cttctttcag cctgcttccc gttgcatact ttactgcatt
    11941 tcttatgtat aatgtatagg cctttccggc ctcatattgt gcaagaggct ctataattat
    12001 cgatttccat tcgcttccca tcttcctgct caaaggtact ttgcttccct gatcatccat
    12061 gacatagaca ttgcctgtac tgactgtcga tgccatcaga ggagcatcaa aggggatctc
    12121 cagtacaatc tcaggagatg ttccgatgcg ctccggcagt tcaataaaag tatcctccct
    12181 ggcaaatgta agaacaggca aagctataca gactgccaaa atacagacca ggcaaattct
    12241 gacggctttc ctctcaatct tcattaatcc tcaccctcct aaaacttgta taatcttatt
    12301 atacaattag acgaagcttt taggatatgg ttcccaatta tctcgtaaaa catcctttat
    12361 gactctttcc ccgttcctgt acattatcct ttcaatatcc ccgccggaaa aacccgcttt
    12421 tgacaatgca tcactcagca gatgcatatc cccggtattt ttgattgccg taagagtgct
    12481 gcagccgtca aagtcagtac ctatggctac cgcttcaata ccggcggtat ttgtcatatg
    12541 gactatatgc cttgtcatgg cttccaaact gccgccatct ccgagaaatt caggctcaag
    12601 gttcagggcg gcaacccctc ccttttcggc aagcttttta agcatgtcgt cagtaagatt
    12661 cctgcagtga ttttcaacag cccttgcgtt ggagtgggac gcaacaaaag gtttttggga
    12721 cagcctgaca acatcataaa agccttgatc cgaaagatgg gatacatcta taatcatccc
    12781 cagtctgttc atctctgcca caacttcttc tccgaaagcc gtaagcccct tgtctttgta
    12841 ctcccatttg aagttcggat agccgatgca gttcggatag ttccaggtca gtgtaataag
    12901 ccttaccccc agcctgtata gaattctgag gttccggagc ttcccttcca gtactcctcc
    12961 ttcctcggca gtaataaatg cggatatttt tccttctgcc ctgtttttct tcagttcctt
    13021 catgcttcct gcaaaagcta tatcggcact gttttcttca atttcattat agaagcagtc
    13081 aatcatatcc aggcatctgt ccatcggcgc actgtactta tccagatcaa catacatcgc
    13141 aaaaaactgt gccagagagt cggccttctt tagcttccgt atatcgatgc agaggtcgtt
    13201 gttccttagt tttacttccc cgtcagagtc tatgcagctt aaaatggtat cacagtgaaa
    13261 atcaataagt ttcatagtat ttcccccggc aaaatttata tttactactc ttttatttta
    13321 acagataccg ggactttttc atatgatggc gatttaatat agtgaaattt cccctgttgc
    13381 tatataatta tattattaca tatgaggagc tgattttatg ttcagagaaa tgagaaggca
    13441 ggacagaaaa acagataatg aaaaagctgc tgcaattctg gctgcgggag aatacggcat
    13501 actctcaacc gtgggagaca acggctatgc atacggagta cccttaagct atgcctacag
    13561 caaaggaagc atatatttcc acagcgcaac ggttggacat aagctggaca atataaagca
    13621 caacgataaa gtatcttttt gtgtggttgg agcaaccgaa gtcatccccg aagattttgg
    13681 cacaaaatat gaaagtgtga tagtattcgg aaaggcttcc gagatatatg acgaagaaaa
    13741 acaagaggct ctaatgacta taatcaagaa atattcatct gattttattg aaaatggcaa
    13801 aaaatatgta gccggcagcg tcagtaaagc aaaagtaata aaaatagaaa tagaacatat
    13861 gaccggaaaa gccaaaaacg attagatttg ccattatgta tccgcaagaa taagcatgag
    13921 ttacttctgc caatgctgtt tattgtttaa tcaggaaaaa aatgctatac tgaaagcaaa
    13981 atatcttccc ggatatcaag tgtatagaat tttaaagcag gtgttgtatc aatggcgttt
    14041 gaatatgagc ttgtgtacaa taatgaaagg ttttatactt gtcttgtcct gctttttagc
    14101 attattctga cctcagttat tataacctat actctgttca gagtaagaag caaggaacaa
    14161 agagccggct ctttgttcct taatgtaatt atcctggttt atcagacaag tcaattcctt
    14221 gttgttgtat cacctcccag ggaattatat ttcttcgtat cattgcaatg tatcggtttt
    14281 tgcatactgg gacctgcctt ctatttatat tgccgcacat tgttaaaaat gggtgctata
    14341 agtttaccag aaacaattgc tttatattct gtgtcctgcc taggaatact gacggtactg
    14401 actaatggtt ttcatgaaat gtttttcaga atttatgata tgttttatat agactacgga
    14461 ataatgtact tcatggctct gatatccaat tctctctggg caatggcagg aagcagtctt
    14521 ttgatgaaat atagagtgac tcatgaagaa tataatatac aacaaagatt tttgtatccc
    14581 atatgcttgc ttgcagttat cggagcaggt gcatttgacg gaatcaatcc tgagcttttt
    14641 tcttatggtc tcactccttc aagtatttca atcatacaaa tccttttcct tttttcaaat
    14701 cgattattag gtctgagcgg taacttattc ataggcaaaa gtaatatatt ggactgcatt
    14761 aatgagtcag tcataatgac tgatacggaa ggaaacataa tattctataa tgatactcaa
    14821 ttaaacagac agatagggat ggaaaacgga gccgatattc aagagatatt ggataaactc
    14881 acccataagt atgaatattc cgatgggctt gatactcttt atgattatct gaatctctat
    14941 aagacacagc ataatacaca taaagatact gctgccggaa caatcccaaa cagcatatgc
    15001 ggggaaatta ccatagaaag ctccggtaaa aggtggtact tctataccgt acagccgata
    15061 acaaagggga aaatccatgt tgcaggtgtt ctttatgttt tcagggatgt tacagaagac
    15121 aaagtgctta taaatcacct taatgacaga aacagcaaat tagcagatac atatgaaaaa
    15181 atgaagaaat attcagcaat tatcaggcaa ttatcagtag aaagggaaag aaacaaaata
    15241 ctcaaaaaga taagcagaga tatagaagaa tctgtcttga aaataatttc tttgctaaat
    15301 gaaatagaag ctatggatga taaagatgcc cgcagtgttg aaataagctt gcataaatca
    15361 atagcgattg caagaaatgg aatagcggga atccgcaaat cgatttctgc aatctcggaa
    15421 ccaataatgc aggaaggaga gaagtgaatg attcgagtga ttattgcaga tgaccattca
    15481 atttttgccg aaagtctgca gcttatgctt caacaggata atgacattgc ggttgaaggt
    15541 attgcattaa atggcaagga tgcagtgaca ctttgcatgg atctgctgcc tgatttagtt
    15601 ctgttggata tcaaaatgcc tggatataat ggcattgaag ccgcaaaaat catcaaaaat
    15661 acttttcccg acataaaaat tgccattctt actacctttg aagactttga aaatataacg
    15721 caggcattta ttaacggaat taacggttat attcttaagg atattacgtc aaaggagctt
    15781 gtcttggctg tgaaatgcat aatttgcggc tttcaggtaa tgcacggtcc cgcaggagaa
    15841 ttcctgcaaa gggaattcat acggctttcc aataatatgg gatatcaaca gcagcagtta
    15901 aaggatgaag atattaagat aattaaatta ataagcgatg gcaagagcaa cagggaaata
    15961 ggagagttaa tgaactacac tgaaggcact attaagaaca gagtatccag gcttatagag
    16021 ctttttgaag tcaaggaccg tacccaggtc gttgtacatg ctttaaaaaa caatttgata
    16081 tgaggctgat attatgacct ttgatattga atggttatat gatatttata tattggagat
    16141 ctggcatata ctttccataa tcttttctgt aatctttgta ctcagtatat ttcttaatgc
    16201 gcgcaagtcc ccggttttat attattatgt tgttttgcat tcaatactgt tggagtggat
    16261 gatctgcaaa gtattaaaaa ccattgctcc tactgtggaa ttaaaatggt tttttattgt
    16321 cgcccaatat tttgccgtat gcttccttgg aagtgcgctg cttatgtttg gttattatta
    16381 taataagaga cggcatttgc ctctttgggc aggtatattg ttgaatattc ctccggttct
    16441 tttctttatc ggaattgtca ctaatgagtc tcatcattta ttttattcaa cctatgattt
    16501 tctcggagat acctttggac cattgtttta tgccagtgcc ggagtaatgt acctgtacct
    16561 tttaatcgga atatattttt gtgcaacttg tttcagaaaa cagcccggga ataagaatac
    16621 ccaggcaaag cttcttacag cgggaatcat ggtgcctctt cttgtaaatg ccctatatat
    16681 attcggattg gttgaacccc gatttgattt tacccctatc agctgcaata tatcgctgct
    16741 gttttttgcc tatgccattt acaaatacca gttccttgac gtaatcaaca tagggatatc
    16801 attatctctt ggcagcatgc aggaaggagt gctggtcaca aataaaagag gacagataat
    16861 agattataat aattcaatta aggaaattct ttgcttttca accaacgaaa ttccctcagg
    16921 cactattgac cgtctttatg attcctttga attgattgat aatgacagca tattaaaaac
    16981 ctgcacatat gacggaaaaa tccattatat taaattctat tcgatgccct tgatatcaaa
    17041 agagaaggaa gcaataggaa atgttcacgt attcacagat ataaccgctt ataaggagct
    17101 tgtcttttct ttgaaaaaaa agaaccggga acaagttgag gcaaataaac agataataaa
    17161 gtacataaat gatgttgagc agcttacagc tatagaagaa cgaaacagga tgtccaaaga
    17221 aatccatgat atattgggac attctctcac attggtcctg gcaattctgg agagcagcct
    17281 aatattggtt aacgaaaata tatccgaagc caaaaaaagg attattcaag ctttatccgc
    17341 tgccaaacaa ggacacagtg aaatgaaaga tgttctcgac ggtaaagcag atctaagtat
    17401 aaagtctacc cgccgactgg aaatggacct ggaaaacctg attgatagtt ataaagttgc
    17461 aggaatggaa gtatatctaa gcatatcggg aacccattta aatattaacc aacaatgcta
    17521 taatactatc ttcaggatat gtcaggaagg tctgacaaat gcaataagac atggaaatgc
    17581 aaagaaagca gatatattta taaggtttgg cagaaaggaa gcggaagtat ttgttattga
    17641 caacggtcag ggctgccatg gtataaacaa gggtcaaggt ctctccggta tggaacaaag
    17701 gattgcagat cttaaaggta ccctctctta cggttcttcc gaaaacaccg gttttactat
    17761 ccacgccttc atacctcttc aagcataagg aattcacata taactaaagt cattcatttt
    17821 attatcctta ggtcattcaa ttcaattact tttgcagctt gcggtgaaaa gctgtaataa
    17881 cttacacttt ctgtaaagac aaatcatttg cgtatattgt gccggctttg acatgtgcga
    17941 tttttttcga ggaggtataa atggaaaatg aaagtcaaaa tgctgctgtt actgttggct
    18001 cttctttgcc ttgtcggatg caaaggtgtg aaaacagagg agaagcagaa cactgatatt
    18061 gaaccgatag aaaaagtgga tgtccgggaa gaaaaagata aagtggtaat ccaagcagaa
    18121 caaggcaaaa tggaaatatc ctctgatgca ggtcaagacc ttgatttgcc tgagggattc
    18181 ccggctgaaa agcttcccgt atatccaaaa ggaagtatag tagtatctga gtgtgtcaac
    18241 aatgtatata atgtaggaat tgtgacaaac gataatgtcg agtcaattta taaatactat
    18301 aagaaaaatt tgaacgcaga cagtattaca tcagacatgc tttcaggtga atcggcaaat
    18361 ctgtttctgc aggatggaga aaaggtagga gccattatga ttatgcccaa taatctgcca
    18421 tacgatttca agtatgtgat ttttatcacc tatggtgaat aaaaagagga gatgataata
    18481 tgaatcttaa gattttttac agaataatcc ccattatatt aatcacacta atactaagtt
    18541 tttcttcagc ggtttctgca tcggctattc cgacccttcc aggcggagct gccgatatcg
    18601 ggccgattat tcccgatatc ccggacgtcc ctatactttt tcttgcacct tcagatctgt
    18661 ctgtcggagc agcaggtgaa acacgagtga atcttatatg gaaagacaat tcaatagatg
    18721 aagaaggctt cagaatattc cggagagaag cgggaggtgc tccatatctt aacattgcgg
    18781 caacaggacc tcatctgtct gaaacaatgg gatatgttga taattcagtt gagagaggaa
    18841 agacttattt ttacaaggta aacgcatata agacaggtac aacctctgct tattccaacg
    18901 aagttactgt tgatgttcct gtgttggcct gccccaatgg actttcagta attaattaca
    18961 catcacgcag cattaccata gcttggaatg acaattccgg atttgaaagc gggtataaca
    19021 ttaatattgg ccgtgaagga agtatgctgt ttaacaggta taccgtaccc gctgattcca
    19081 cttcgtattt attcgagagc cttactcccg gggtactata ttgggtaagc gttgaggttt
    19141 ttgatgaaga agacaacaaa tgtgaaaccg agaaaataac tatgagaaca ccctttgaag
    19201 tattacagcc gctgaatgca cctacggatg ttaatgcaaa tacagtaaca cagaataaaa
    19261 tactcttgtc atggagagac aattctgacg gagaaaccgg atatactgtt gaaagaaagg
    19321 aagctgggga tacagattat gtcattcttg caaatttaaa tacaaattcc gaagtatata
    19381 atgacaatac ggtaatcaga gacaaaatat acatgtatag ggttaaagtt ttaaaagaca
    19441 tgcaatatga atgctcagag ccttttaccg taaacacctt cattccggaa gctccttcag
    19501 gcttgaacgg agtagaaaga gaagacagct cctataggct tacatggatg aatgatactg
    19561 attatgaaac cgcctacagg gttgaaagaa aaacttatga cggcacttat tccttgttgg
    19621 gttattccgg accggatact cttatatatg acgaccatac cgcccggcct gatacagtct
    19681 attgttacag agtgtgtgca gtaaacggcc aattggtatc cggttattcc aacgaatgct
    19741 gtctcggtat gcaagcgcct ccccatattt ctcccgaaga ttctgaaatt cccgctgaaa
    19801 caccggttga agattaccac gaaaattcag gggggacatc agtcgactca gaaggaacat
    19861 caagggattc agaagattca gcagacacag gcattttcga cggcacacaa agccaatggg
    19921 cagcatcgga gcttgaaaaa gcttttgatc acgaccttac ctacccggga ataatgaaca
    19981 actataaaaa ccaaattacc agagaagaat tctgcaaaat agcagtaaaa ctatatgagc
    20041 agcttagcgg agcaatcgct ttagccggag atgatccttt taacgatact gatgatcctg
    20101 agatcttaaa agcatataag ctgcaaatcg ttatgggaat tggaaatggt cagtttgcgc
    20161 cacataacaa aatcacacga caagaaatat gtgtaatgat aatgcgggct cttgatgctg
    20221 caatacctga agttgacaga agtacagctt ccgttttccc ttttgccgat aaaaacaata
    20281 tagccccctg ggctatggat tccgtgaaat tttgttataa aaacaatatc atgaagggca
    20341 tcagcgaaac tgagattgca ccccttgccg atattacccg ggagcaagcc atagtattgc
    20401 tgaaacgaac ctttgaacaa tatatgagca gatagagctt ggtaaaaaag ccgtataaaa
    20461 tcattgattc tatacggctt tagcattcct tgattatcca agctttataa atctgtctat
    20521 atccttctct ataagctcca gagaattt
//
LOCUS       DAOOAB010000046        20089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3555_length_20089_cov_16.827743, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000046 DAOOAB010000000
VERSION     DAOOAB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20089
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_20089_cov_16.827743"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..653)
                     /locus_tag="PKU88_09615"
     CDS             complement(120..653)
                     /locus_tag="PKU88_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPX69571.1"
                     /translation="MQRKLTVKDLSYISLFATLISVSGYIIIPLPFSAVPVTAQTLAV
                     MLAGSLLPVGQTVLSLLVFILMGAAGLPVFSGGSAGMGAILGKTGGYLIGFVAGAALI
                     SALKGKKPGFVRLAAANVLGGIVVVYSFGVFWLNYVTGIGISQAVIFGALPFIPGDIL
                     KVIIASMLSLRLSKHMK"
     gene            complement(799..2052)
                     /locus_tag="PKU88_09620"
     CDS             complement(799..2052)
                     /locus_tag="PKU88_09620"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPX69572.1"
                     /translation="MAHQDNLNPFLNAQKQVKTACDKLGLDPKVYEILKQPMKVLEVA
                     IPVKMDDGSLRIFTGYRAQHNDAVGPTKGGIRFHQNVNRDEVCALSIWMTFKCCVTGI
                     PYGGGKGGIIVDPTTLSQGELERLSRGYIQAIYKLIGEKVDVPAPDVGTNGQIMAWMV
                     DEYNKLVGRSELGVITGKPVEFGGSLGRNAATGFGVAVVAREAAKKVGIDFKGASIAV
                     QGLGNVGSHTALNLQKLGGKIVAVCEWNGSIYDEKGLDINALVAYMKQNKKIVGFPGS
                     KEITKAEFFALPVDILVPAALENQITAEVAETIRAKLVCEAANGPTTPDGDEVINKKG
                     LLLTPDILTNAGGVTVSYFEWVQNLYGYYWTEKEVEEKEERAMVEAFNSIYAIKEEYN
                     VSMREAAYMFSIKKVAAAMKVRGWL"
     gene            complement(2261..3310)
                     /locus_tag="PKU88_09625"
     CDS             complement(2261..3310)
                     /locus_tag="PKU88_09625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPX69573.1"
                     /translation="MSDYYVIIPGNASLQLKKMLEAVLTGKNASYITDKQSLPDLRGV
                     KIIVCAEISPAGLCIPLYEILEELQSRGCDSLKDSSAILLLHSSNELYTKSTASLIVF
                     LMNQLGCRFPGHPAIEATGSLLNFSAWRKIKEASLEDICLELCTSLGERFAAYNPAAV
                     KKPKILALHSSRKETSNTLALWNMVKNSLEGCRISELHVENGSVVDCKGCSYKTCKHF
                     SMNNSCFYGGIMVKEILPAIEKSDAVIWLCPNYNDAVSANLSAVINRMSALYVKTPFY
                     NKSLFSIIVSGNSGSDSVARQLLGALCINKGFNLPPYHSFMATANDPGTALRIPGIEA
                     AAKKFSENIVNELKI"
     gene            complement(3307..>3636)
                     /locus_tag="PKU88_09630"
     CDS             complement(3307..>3636)
                     /locus_tag="PKU88_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HPX69574.1"
                     /translation="GEGLVIADSIKNYNDRLSRYLTEKSKTANLEVRKLGYKPYIAPA
                     LSSGALSILATIRGQWHYSATFLGGVYMGARNRLNASGIELERLPVPEELFLRLKNTY
                     ESLRKMP"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3737..4730)
                     /locus_tag="PKU88_09635"
     CDS             complement(<3737..4730)
                     /locus_tag="PKU88_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HPX69575.1"
                     /translation="MKYIGGNMMFYYKLFNKTLVSRKKYLQLEEIPEEEAASGAGRIF
                     VLDSCPPGESRRSFCASQPDLVYNLKEGLDMLLLPDSEDNNLPDWIIKAVSGNNVTRI
                     NTEYPNWQDVLLYEPPAKWRINVAGLGDVGGTLVSGLRLLGGGSVSHIGIFDIDPNKV
                     RRWEYEANQILDPSCGNAHPDVVSISEKQLFDCDMFIFCITAGVPPLSENPGDVRRAQ
                     YEGNSKIIWEYAQKARKCGYKGIFAVVSDPVDMLCKAAFGFSNTDGCGRLDFNGLAPE
                     QIRGYGLGVMHARAAYFAGLGRETAHYIREGRAFGPHGEGLVIADSIKNYNDRLS"
     gene            complement(4840..9765)
                     /locus_tag="PKU88_09640"
     CDS             complement(4840..9765)
                     /locus_tag="PKU88_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HPX69576.1"
                     /translation="MNKEYVIRLYNDICNRLLRLDLPDNSKDAAKKYIEDVSFIENLH
                     KIVRNRDYSCNSVLLLCQSLMNQVAEDNIPSNWLEYVYQYTLSKSFPHAVEAELKNGL
                     EGSCSIYLQTLREISEFQKNGSDNTWQSRYPIRFLSSEEENELENPIEYKKFLKAFDE
                     LYVYEMMKLNQEVLGFNTLDHICGVHYLALYIGRELKKAGIPIDLGRVSGAAAGHDIG
                     KYGCKSSESKRVPYLHYYYTDLWFRNHDITYIGHIATNHSTWDLELENLPLESLVLIY
                     SDFRVKHDATGNTREMHIYPLKDSFNIILDKLDNLDAIKEKKYRRVYSKLADFEDYLI
                     SLGIEVDINSISKGIRRELGIKNRHYSLMSGNQVIKSIKHLAISHNINLMYQLRDEAS
                     LNRILESARSEANWKKLREYLRIFEEYSTYLTQKQKLITIRFLYEQLVHPEDDIRRQC
                     AELIGLLIARFDEKYRKDVPENAILDTPVMTSFELLDKYMQLLVSPDHKIVPLHKSYL
                     GYSISIIVSSLFSHCGKSQTEGFRTVAAKYYKEGARRNNETKLYLLEALRYMPVCGDH
                     EADRIICSYVKRRLSDKSNAIRLSAMDVAYNILARSRKNTDTSGDNTAIGSLALSVKE
                     MISGTHRRSGVVSENYLKHKIACLLEMEPAVLNKFHEYCHKDLHKLSEISLSNLKTAT
                     EWVIKRIQLDLLLDNSLSNPKSNGFYSAMHFCNLMKVSASESVRNRAGEALTHIVSHL
                     SPEQRNDIAVEMLRALEIEGYQFAEYIPNYLGKIILSLEPVELDEVLDDFKEKIKNST
                     PHLDSLILKTIGITIANYPSYIKTFKEKREVFENRLIRILSIIINGLAHYDADVKQSA
                     FSVLGKEIFGSSTLMPEEKNEIFIRIAKKLLTLLEDDIREGLLFLTNSAGLINIYRFI
                     SDYTFFYKDIEIAVPEKVALFPGAFDPFSLGHKEIARAIRDMGFEVYLAVDEFSWSKK
                     TLPNLIRKSIINMSVAEEFNIYIYPEDYPTNIANPLDLEVLRNNFPASEVYIVAGSDV
                     MINASAYKKEISKNSVLTFSHIIFERKSSYNQYEKDAGKLSETIGKIDGKVIRLSLPP
                     QYEDISSTQIRSNVDENRDISMLVDPLAQKFIYENGFYQSEPQYKSMVQTESIEIEVV
                     EKASRELFNELSSSLCIDKESLADSLGKFLGKIASRLLLIRDADHKRQILGFSAFHRI
                     SSSFLYQEFGNSSVTDYIRENSTGKIVSIDGLFILNNTVLENMEQILLAETLAYCIAR
                     DYDYAVFHSTLSPISYDMLEALRLMGFRDLPCPGCLNKVLAVNISTPCALNLDIETII
                     KEPYVSSSSFKDIVSRTRKRLQSTLAEVYAGNLILPFDRAVMDSTLVKKICEVNVVPT
                     KPLKPRHLGPAMCVPYGNILSRSIIPNTVTKALHTERLYGPDAKDYKVGSFPYYLDLE
                     IQMKMLKSFDRPIILVDDTLHHGFRLKTLDPMLKKENIEIRKIIVGILSGKGKELMEI
                     QGNDTDCAYFIPRLREWYNENNFYPFIGGDALWRGIYPKRNLLPSINLILPYTSPSFL
                     NETSKKSIYMLSHTAMENAIEILTTLEDEFQAINERSLTLSSMGQVFITPRCPDHGKN
                     MDYDLSLSPSFYLKNDLELLDRLERIICNIF"
     gene            10307..11023
                     /locus_tag="PKU88_09645"
     CDS             10307..11023
                     /locus_tag="PKU88_09645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPX69577.1"
                     /translation="MGKKQRIDKILSNMGYGTRKEVKELISGGLVEIDGIVVKEPGQQ
                     VTADSQEIKVGGRKLLYKDFIYIMMNKPQGVISATVDSREKTVIDLLPDEFKAFKPFP
                     VGRLDKDTEGLLLLTNDGQMAHRLLSPKKHVPKTYIAKVAGRVSQEDAEAFGKGVILE
                     DGYKTMASKLQILVRGSISHVEVTIYEGKFHQVKRMFQAVGKEVIFLKRISMGELKLD
                     ETLEPGEYRELNEEELRILS"
     gene            11131..12159
                     /locus_tag="PKU88_09650"
     CDS             11131..12159
                     /locus_tag="PKU88_09650"
                     /inference="COORDINATES: protein motif:HMM:NF039924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPX69578.1"
                     /translation="MAMEIYGESDEPLVRIVNSPVVELICAVHLLTDAAHHQYELRWA
                     EEVLSGFKQEELEALNTIKLFSSGWMNMLNLILESGILTSIPEFIGYIERLDLLDFFY
                     FFLNEEVERTKIQKAFAYKKERSRIQEKTKWAVEESELMEFFEFPEKIKRDTINLFKK
                     VYAHDFMAKLDRCKEKMEAGIQYVESFTGKDSVESVLEKISGRSRKLFSGFKEYYIIP
                     SYFISPQKSLRIYSGDRIYVVFDCRVTRDKREALLEELSSVLKIIDDKSRMEILRILM
                     NNKSYGKALSDLVGLSTPTVSHHLDILKQAGLIKEEKIRNIKYFYADKDKLKKVVDDI
                     NKYFFGAE"
     gene            12300..13307
                     /locus_tag="PKU88_09655"
     CDS             12300..13307
                     /locus_tag="PKU88_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPX69579.1"
                     /translation="MKIREITENMEKNNLSKYASLSVNSKGRQTWEEKCDIRTDYQRD
                     RDRILHSKAFRRLKHKTQVFISPEGDHYRTRLTHTLEVSQIARTIARGLRLNEDLTEA
                     IALGHDLGHTPFGHAGEKVLNEIHPVGFKHNEQSLRVVDILEGGKGLNLTYEVRDGIL
                     RHTGDIGPETLEGNIVSFSDRIAYINHDIDDALRGGILEMSSLPEKCIAILGDTHKKR
                     INTMITDIITQSMDKNEIMMSSMVRQATDELREFMFENVYIGSEAKAEEKKTRNIISC
                     LYEYYVQNPEEMPIENIRENRDADNIDIERLACDYIAGMSDRFAINKFTELFIPSPWR
                     V"
     gene            13413..13865
                     /locus_tag="PKU88_09660"
     CDS             13413..13865
                     /locus_tag="PKU88_09660"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HPX69580.1"
                     /translation="MRILIDGDACPVTHLIEKTAARCGVGVVFFHSTCHYSNYSNAAA
                     EKIMVDNESQAVDMAIINKAVKGDIVVTGDFGLASLTLGKGAKAISFSGMIFDESNID
                     RLLFERHVSGVIRRSGGRTKGPARRTDSDDRNFEKSLSFLISQIMKDI"
     gene            13945..15714
                     /gene="dnaG"
                     /locus_tag="PKU88_09665"
     CDS             13945..15714
                     /gene="dnaG"
                     /locus_tag="PKU88_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPX69581.1"
                     /translation="MFFSEDKISEIIEKNDIVDVISEYMTLKRSGSNYMALCPFHSEK
                     TPSFSVSPSKQIFNCFGCGFGGNVITFIQKIERIEFVEAVKMLAEKAGVKLEEDKDPK
                     QLERAKQKKALYRINAEAARFFYSNLNAGSKALEYLRRRGLSDAIIKRFGLGYAPDEW
                     YGLMNHMKRKDIDTELLFKAGLIVRNNDGEKFRDKFRNRIMFPIIDLKGNVIAFGGRV
                     IDDSKPKYLNSPDTLVFNKGYNIYGLNFVKKVQNLENIVVVEGYMDAIALHQFGVENA
                     VASLGTAFTENQAKLLKRFSNEIIIAYDSDLAGQAATLKGLAILEKEGCVVKVLSLPS
                     GKDPDEYIRKEGIDAFRDRIRKSVSLIEYQIENLKRGLDINNIQDRIKFTKGFSEILK
                     NVVSNVEVDAYIRKYSGIMKVNEEALYAELNRLRKKNKNGNNKHNILNSIKTEPELKK
                     GEITAEIYLLNICIYYFKKVKNIFEILKPADFSNDIHVRIAEIIQRAYKEDKAITAGE
                     IISYFDNDDEKSRVAGIFSQKLPDSDVDGLIKSSLDKIRQSKLDRTIQEMTEKMNEMY
                     HAGKKEEANKIFVEIKELQKKKR"
     gene            15758..16825
                     /gene="rpoD"
                     /locus_tag="PKU88_09670"
     CDS             15758..16825
                     /gene="rpoD"
                     /locus_tag="PKU88_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPX69582.1"
                     /translation="MTYVKELVDKGKKNGSLTYKEIMDTFEEVEMDPEQIDKLYEYLE
                     TKGIDVVGAIDNDMDIPPLDEDVEEVDLDISIPEGISIDDPVRMYLKEIGKVPLLSPE
                     EEIDLANRIKKNDSEAKKKLTEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVE
                     KFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQLLQQLG
                     RDPIPEEIAAEMGMSEDKVREIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAE
                     SAAFTLLKEQLMEVLDTLTPREEKVLKLRFGLEDGRARTLEEVGKEFNVTRERIRQIE
                     AKALRKLRHPSRSKKLKDFLE"
     gene            16888..16963
                     /locus_tag="PKU88_09675"
     tRNA            16888..16963
                     /locus_tag="PKU88_09675"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16921..16923,aa:Asn,seq:gtt)
     gene            16969..17045
                     /locus_tag="PKU88_09680"
     tRNA            16969..17045
                     /locus_tag="PKU88_09680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17003..17005,aa:Ile,seq:cat)
     gene            complement(17098..17289)
                     /locus_tag="PKU88_09685"
     CDS             complement(17098..17289)
                     /locus_tag="PKU88_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69583.1"
                     /translation="MSKKLHIKPEVIQKFSWGGTTKEWEKEAEKLSNQLETMEAINNI
                     IEKQDCESEIKKPPGGGNK"
     gene            17403..18137
                     /locus_tag="PKU88_09690"
     CDS             17403..18137
                     /locus_tag="PKU88_09690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX69584.1"
                     /translation="MQQQLYINNVRMMILNLTPRLKIIADSIQGFETLADIGSDHAYL
                     PIYLIKNRRVKSVIATDVNSGPAEISRKRIKNHGLESKISVREGSGLRVINSGEAEVI
                     VIAGMGGILIGDILDKGIDAAKSAKLLILQPMRDSEKVREWLLEHGFDIIDEELVKDQ
                     DKIYEVIWSQPGEEAGEAKGPLLIGEKIIEKKHPLLEEFINRKVKELEKIMIALEAMD
                     SDNCRERAEECRAMLAYYREVAEWAR"
     gene            18125..19246
                     /locus_tag="PKU88_09695"
     CDS             18125..19246
                     /locus_tag="PKU88_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPX69585.1"
                     /translation="MGTIKCQTIAGMLDELAPRSLAESWDNVGLLVGDGRKRVSRIMV
                     CLDVTEWVIEEAVDNNVDMIISHHPMIFSGIKKVNTDTATGRKLISLVKNDISLYCLH
                     TNFDIVSGGLNDIFAKELGFSHWTIIEETYREKLFKLVVYVPAGQEDSVADAMCRAGA
                     GYIGKYSGCTFRSKGIGTFEPQEGAKPFLGSIGNLERVEEFRVETIVPEKLLSKVVGE
                     MLKAHPYEEAAYDIYETKNEGRVMGLGRIAELERETTLSMFAGNVKTLLNAENIRYAG
                     DPNRSIRKVALLNGSGSKFINSARLAGADVLITGDLQYHSTLEALEMGLCVIDAGHFS
                     TEKIMMKYITEFLKNKFRELKVEADVFESCSNTEILRVL"
     gene            19302..20012
                     /locus_tag="PKU88_09700"
     CDS             19302..20012
                     /locus_tag="PKU88_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPX69586.1"
                     /translation="MNLPLLYELQHLNKEMGAITRKLRELQEDKDMKKYKEEYQRLKE
                     EYLKGEEKLKKNAYRQEARNNEIRNIEYNMKSSLEIKFSKETNTVKKLENIEKQLEKL
                     EEKKQAAENEIIELINDADNINKELAEIKKKLAFIKKKFLNCKEDADKAAAGLKAQKS
                     ELQAKIDSMMKSIDQESLEMYNRLIKTHSDPVALVKNRICSGCKMEVPAIDYEALKGG
                     NQELKCQSCGRILFYKKS"
BASE COUNT         5937 a         3875 c         4574 g         5603 t
ORIGIN      
        1 gacacggggg agacacgggg gagacacggg gacgtttaac gtgtccctca agggacacgt
       61 taaacgtccc cgtgtctccc ccgtgtctcc cccgtgtctc tcccaccccc catgtcccat
      121 catttcatat gcttggaaag ccttaaggaa agcatggatg caattattac tttcaaaata
      181 tctccgggaa tgaagggcag tgcgccgaat atgaccgcct gagaaattcc tatgcctgtg
      241 acataattca gccaaaatac tccaaaggaa tacaccacaa caatcccacc caatacattt
      301 gccgccgcaa gccgtacaaa acccggcttt tttcccttca gggccgatat cagggcagca
      361 ccggcaacaa agcctatcag gtagcctccg gtcttgccca aaatagcgcc catgccggca
      421 ctgccccctg agaatacggg aaggcctgcc gcacccataa gaataaaaac caggaggctt
      481 aaaacagttt gcccgacagg aagcaggctt cccgcaagca ttaccgccag ggtttgtgca
      541 gttacgggaa ctgcggaaaa aggaagaggt atgataatat atccggaaac agaaatcagt
      601 gtggcgaata acgatatgta actcaagtct tttactgtta gttttctttg catatgtatc
      661 ctccaagaaa ttatatgaat atgctatata taccattgta cacattgata atgccaattg
      721 tcaaccacaa aaataataag gttgacaatt ggccgataaa agaggcctgt gataattcac
      781 aggcctccgg ttgttttctt acagccagcc cctaaccttc atagcagctg caaccttctt
      841 gattgagaac atgtaagctg cttctctcat tgaaacattg tattcttctt ttattgcata
      901 aattgaattg aatgcttcaa ccattgctct ttcttctttt tcttcaactt ctttttctgt
      961 ccagtagtag ccatacaggt tctgtaccca ttcaaagtat gatactgtaa ctccgcctgc
     1021 attagtcaat atgtcaggtg tcagaagaag tcccttctta ttgataactt catctccgtc
     1081 aggagttgta ggtccgtttg cagcttcaca gacgagtttt gctcttattg tctctgcaac
     1141 ttctgctgta atctggtttt ccagagcggc aggaacaaga atgtcaaccg gtaatgcaaa
     1201 gaactctgcc tttgttattt cttttgatcc cgggaagccg acgatcttct tgttttgctt
     1261 catgtaagct accaaagcgt tgatatccag gcccttttcg tcatatatgg atccgttcca
     1321 ttcgcaaaca gcaacgattt ttccgccaag tttctgaaga ttcaatgcag tatggcttcc
     1381 gacattgcct aagccctgta cggcaattga tgcgcccttg aagtcaatgc ctactttttt
     1441 agcagcttct cttgctacaa cagcaacacc aaagcctgta gcagcgtttc tgcctaatga
     1501 accgccgaat tcaacgggtt tgcctgtaat aactcctaat tcactgcggc cgaccagctt
     1561 attatattcg tcaaccatcc atgccataat ctgaccgttt gttccaacat ccggtgccgg
     1621 aacgtcaact ttttcgccta ttaatttata tattgcctgt atgtatcctc ggcttaatct
     1681 ttcaagctct ccctgagaca gtgtggttgg gtcaacaata attccgcctt ttcctccgcc
     1741 gtaaggaata cctgtaacgc agcatttaaa tgtcatccag attgataagg cacaaacctc
     1801 atccctgttt acgttctgat ggaatctgat tccacccttt gtaggaccaa cagcatcatt
     1861 gtgctgagct ctgtatcctg taaagattct cagtgaaccg tcatccattt tcactgggat
     1921 agctacttca agtaccttca tcggttgttt taagatttcg taaacttttg gatccaaccc
     1981 taacttatcg caagctgttt ttacctgctt ctgagcattc aagaaagggt tcaaattgtc
     2041 ctgatgtgcc attttttatt aacctcccaa ttgtaattat taatataata agcaaattat
     2101 gagaaacata attgctgatt atcaagccgg ttctgattca atcacttctt atggcatgta
     2161 cattttcatc caaaaaaata gtatagcata ttaatccatt cagatatata atacaacatt
     2221 tctatataaa aatcaaaact cctgctaaat ttttaacaat ttatattttt agctcattta
     2281 cgatattttc tgaaaatttc tttgccgctg cttcgattcc gggtattctt aaagctgtcc
     2341 ccggatcgtt ggcagtagcc ataaatgaat gatagggagg cagattgaag cctttgttta
     2401 tgcaaagtgc ccccagcagc tgcctggcta ccgaatcact gcctgaattg ccggatacga
     2461 ttatggaaaa caatgattta ttatagaaag gtgtttttac atataatgcc gacatacggt
     2521 taatcactgc ggaaaggttt gcggataccg cgtcattgta attggggcaa agccatatga
     2581 ccgcatcaga tttttctatg gcgggcagta tttccttaac cattatgccg ccataaaagc
     2641 agctgttgtt catgctgaaa tgcttgcagg ttttataaga acacccttta caatcaacta
     2701 cactgccgtt ttccacgtgg agttcgctta ttctgcatcc ttcaaggctg ttttttacca
     2761 tattccacag ggcaagtgta tttgaggtct ccttccggct ggagtgaagc gcaagtattt
     2821 tcggtttttt aactgctgcg ggattgtatg cggcgaatct ttctcccagt gaagtgcata
     2881 gctccaagca gatatcttca agggatgctt cttttatttt tctccaggcg gagaagttta
     2941 acagactgcc tgtggcttct atggcaggat ggccggggaa gcggcagccc aattgattca
     3001 tcaggaatac tataagggaa gcagtactct ttgtatatag ttcatttgag ctgtgcagca
     3061 gcagtattgc agaggaatcc ttcagggaat cgcagccccg gctttgaagc tcttccaata
     3121 tttcatacaa gggtatgcat aagccggcag ggcttatttc agcgcatact attatcttta
     3181 ctcccctgag atcgggtaag ctttgcttgt ctgttatgta tgaagcattt tttcccgtca
     3241 atacggcttc cagcattttt ttcagctgca gagaagcatt tccgggaatt atcacataat
     3301 aatcgctcat ggcattttcc tcaggctttc atatgtattt ttcaatctta gaaaaagctc
     3361 ctccggaaca ggaagcctct caagctctat tcctgaagca ttaagcctgt ttcttgctcc
     3421 catgtatact ccccccagga aggtagcgct gtaatgccac tgtcctctta ttgttgcaag
     3481 tattgacaag gcgcctgacg agagagcagg agcgatatag ggcttatagc ccagcttgcg
     3541 gacttccagg tttgctgttt ttgatttttc ggtaagataa cgggaaagac ggtcattgta
     3601 gttttttata ctgtccgcta ttaccaggcc ttcgccnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnggga aagacggtca ttgtagtttt ttatactgtc cgctattacc
     3781 aggccttcgc cgtgagggcc gaaagctctg ccttccctta tgtagtgagc ggtttccctg
     3841 cccaggcccg caaaataggc tgcccttgca tgcattactc caaggccgta gcctcttatt
     3901 tgctcaggcg caaggccatt gaaatcaagc ctgccgcagc catcggtatt gctgaagccg
     3961 aaggccgcct tgcataacat atccacagga tccgacacta ccgcaaatat gcctttatag
     4021 ccgcatttcc gggctttttg ggcatattcc cagatgatct ttgaatttcc ttcatattgt
     4081 gcccttctta cgtctcccgg gttttcgctc agcggaggca cccctgcggt tatgcagaat
     4141 atgaacatat cacagtcaaa aagttgtttt tccgatattg aaacaacatc aggatgagca
     4201 ttaccgcagc tggggtccag tatctgattg gcctcatact cccaacgcct gactttgttt
     4261 ggatctatat caaaaattcc tatatgtgat accgagccgc ctcccagaag cctcaggcct
     4321 gagacaagag ttcctcctac atccccaagg cctgcaacat ttattctcca ttttgcaggc
     4381 ggctcgtaaa gcagcacatc ctgccaattg ggatattcgg tattgattct ggttacatta
     4441 ttaccgctga cagctttaat tatccaatcc ggcaggttat tatcctctga atcgggaaga
     4501 agaagcatat caaggccttc cttcaagtta taaacaagat caggctggga agcgcagaag
     4561 ctccttcttg attctccggg agggcagctg tccagcacaa atatacgccc ggcaccggaa
     4621 gccgcttcct cttccgggat ttcttccagc tgcaggtatt ttttccggga taccagggtc
     4681 ttgttgaata gcttataata aaacatcata ttacccccga tgtatttcat atagaatacg
     4741 cctgtgtgca gccgattcat tataactggt agtcttgacg tattctatga atcaaggaat
     4801 atattttagg atatccgaaa catgtatcaa atgtatatcc taaaatatat tgcatatgat
     4861 tcgctcgagc ctgtccaaca gctccaaatc gtttttaaga tagaaggatg ggctcaggct
     4921 aaggtcatag tccatgtttt tgccgtgatc gggacacctg ggtgttataa acacctgccc
     4981 catggaagac agggtcaggc tcctttcatt aatggcctgg aattcatcct ccaaggttgt
     5041 cagtatttct atcgcatttt ccatggcggt atgcgaaagc atatatattg atttttttga
     5101 tgtttcgtta agaaaagagg gtgaggtata agggagtatc aggtttattg agggaagcag
     5161 gttccttttc ggatatatgc ccctccataa agcatctccg ccaataaagg gatagaagtt
     5221 gttttcgtta taccattctc tcagacgcgg aataaaatat gcacaatccg tatcattccc
     5281 ctgaatttcc attaattcct tcccttttcc ggaaagtatg ccgactatta tcttccttat
     5341 ctcaatattc tcttttttca gcattgggtc cagggttttc agcctgaagc cgtgatgaag
     5401 ggtgtcatcc acaagaataa tgggcctgtc aaaggatttc agcatcttca tctggatttc
     5461 aagatcaaga taatacggga aactcccgac cttgtaatct ttagcatcag ggccgtatag
     5521 cctttctgta tgaagtgcct ttgttactgt gttgggaata atgcttctgc tgagtatatt
     5581 gccgtagggt acgcacatag caggccccag gtgccttggc ttcaaaggct ttgtgggaac
     5641 cacattcacc tcgcatattt ttttaaccag agtgctgtcc atgactgccc tgtcaaaggg
     5701 cagtatcaga tttcccgcat atacttcggc aagagtgctt tgaagcctct ttctggttct
     5761 tgagactata tccttaaaag agctgctgct tacgtatggt tccttgatta tggtttctat
     5821 atccaggttc aaggcgcagg gggtgcttat atttactgcc agcactttgt tcaggcatcc
     5881 gggacagggc agatccctga agcccatcag cctgagggct tcaagcatgt cgtaggatat
     5941 ggggcttaat gtgctgtgga atactgcgta gtcataatcc cgggctatac agtaagccag
     6001 tgtttccgcc agaagaatct gttccatgtt ttccagaacg gtgttattca atatgaataa
     6061 accgtcaata ctcactattt ttcctgtgga gttttctctt atataatcag ttacggaact
     6121 gttcccgaat tcctggtaaa ggaagctgga gctgattctg tgaaaagcag aaaaaccaag
     6181 gatttgcctc ttatgatccg catctctgat taatagaagc cttgaggcta tttttcccag
     6241 aaactttcct aggctgtctg caagggattc tttgtcgata cacagggatg aggataattc
     6301 attgaacagc tctctgcttg ctttctccac tacttcgatt tcgatggact ctgtctgtac
     6361 cattgactta tattggggct cactctgata gaatccgttt tcatagatga atttctgggc
     6421 cagaggatcc accagcattg atatatctct gttttcatca acattgcttc ttatctgggt
     6481 agaactgata tcctcatatt gaggaggaag agagaggcgg attacctttc cgtcgatttt
     6541 gccgattgtt tccgaaagct tgcctgcatc tttttcatat tgattatagg aactcttcct
     6601 ttcaaatatt atatgtgaaa aagttaatac ggagtttttg cttatttctt ttttatatgc
     6661 cgaggcatta atcattacat cgcttcccgc aactatataa acctccgacg ccgggaaatt
     6721 gttcctcagt acctcaaggt ccaaaggatt cgcaatattt gttggataat cctcgggata
     6781 gatgtatatg ttgaattctt cggcaacgga catattaata atgcttttcc tgataagatt
     6841 aggcagggtt ttctttgacc aggaaaattc gtccacggcc aggtaaactt caaagcccat
     6901 atcacgtatt gctctcgcaa tctccttgtg ccccagagag aaaggatcaa aagcccccgg
     6961 gaaaagtgca accttctcag ggacagctat ttcaatatct ttgtaaaaga aagtataatc
     7021 ggaaataaac ctgtaaatat tgattaagcc cgctgaattc gtaagaaaca acaacccttc
     7081 ccggatatcg tcctcaagca gtgtgagcag cttcttggca atacggataa aaatctcgtt
     7141 cttttcttcc ggcatcagag tacttgaacc gaagatctcc ttgcctaaaa cgctgaaggc
     7201 agactgtttt acatctgcgt cataatgggc aagcccgttg attatgatac tgagtatcct
     7261 tatgagcctg ttttcgaaga cttccctttt ctctttaaat gttttgatat aggatggata
     7321 atttgctatt gtaatgccta tggtttttaa tatcagagag tccaagtggg gggtggagtt
     7381 ctttatcttc tccttgaaat cgtcaaggac ctcatcaagt tccacgggct caagggatag
     7441 tataattttt cccaggtagt tcggaatgta ttctgcgaac tggtatcctt caatttcaag
     7501 ggcacggagc atttccacgg caatgtcatt cctctgctcc ggggacaagt gggaaactat
     7561 atgtgtcaga gcctctccgg cacgattgcg gacactttcg gaggcactga ccttcataag
     7621 attgcaaaaa tgcatagctg aataaaaccc gttggatttt ggattactta atgaattgtc
     7681 cagaagcaga tccagctgaa ttctttttat tacccattct gttgcggttt taaggttgct
     7741 caatgagatt tctgaaagct tgtgaaggtc cttatggcag tactcatgga atttattcag
     7801 taccgccggc tccatttcca gcaagcatgc gattttatgt ttcagatagt tttcggaaac
     7861 aacgccggat cttctatggg tgcctgatat catttccttg acagagagtg ccaggctgcc
     7921 gatcgcggta ttatctccgg atgtatcggt atttttccgg gaacgggcaa ggatattata
     7981 tgccacgtcc attgcggaaa gcctgattgc attgctttta tcgctaagcc gccttttgac
     8041 atagctgcag attatcctgt ctgcttcatg gtcaccgcag acaggcatat accttaaggc
     8101 ttcgagcaga tacagctttg tctcattgtt tctgcgggcg ccttccttat agtatttggc
     8161 ggcaacagtt ctgaagcctt ctgtctggct cttgccgcag tgtgaaaaca atgaagatac
     8221 tatgatactg atgctgtacc ccaggtaact tttgtggaga ggtactattt tatggtcagg
     8281 gctgaccaga agctgcatat atttgtccag caactcgaag cttgtcatta cgggagtgtc
     8341 aagtatggcg ttttcgggca catctttgcg atatttttca tcgaatcttg ctatcaatag
     8401 tcctatcagc tctgcacatt gccttctgat atcatcctcg gggtgcacca gttgttcata
     8461 aaggaatctg atagtaatga gtttttgttt ctgtgtaaga taggtagagt attcttcaaa
     8521 aattcttaaa tactcccgaa gctttttcca gttggcttca cttctggctg attccagtat
     8581 gcggttaagg gaagcttcat ccctaagctg atacataaga tttatgttat ggctgattgc
     8641 caggtgcttg atacttttga ttacctggtt cccgctcatc agggaataat gacggttttt
     8701 tattcccaat tcccttctta tgcccttgct gatgctattt atatcaactt ctataccaag
     8761 gcttatcaaa taatcctcaa agtccgcaag cttggaatat actcgtctgt acttcttttc
     8821 ctttatggca tccagattat ccagtttatc cagaataata ttaaaggaat ccttcagggg
     8881 atagatatgc atttctcttg tgtttccggt tgcatcatgt tttactctga agtctgagta
     8941 tatgagtacc agggactcaa ggggaaggtt ttcaagctca agatcccagg tggaatggtt
     9001 tgtggctatg tggccaatat aggtaatgtc atgatttctg aaccacaaat cggtatagta
     9061 ataatgaaga tacggcaccc tcttggattc agaagatttg catccgtatt ttccaatgtc
     9121 atggccagct gcggctcccg aaactctgcc aaggtcaatt gggattccgg cttttttcag
     9181 ttcccttcct atatataaag ccaggtaatg caccccgcag atatggtcaa gggtattaaa
     9241 gcccaagacc tcctgattca gcttcatcat ttcatataca tacagttcat caaaggcctt
     9301 aaggaatttc ttatattcaa ttgggttttc caattcgttt tcttcttcag aagataggaa
     9361 tcgtatggga tatctgctct gccatgtatt gtcggagccg tttttttgga attcggaaat
     9421 ctctctgaga gtctgcagat atatgctgca tgagccttcc aatccattct taagctccgc
     9481 ttcaacggca tgggggaagg atttgctcag ggtatactga tatacatatt caagccaatt
     9541 tgagggaata ttatcctctg caacttggtt cataaggctt tggcacagca gcagaacgga
     9601 attgcagctg taatccctgt tcctgacaat cttgtgaaga ttctcaataa agctgacgtc
     9661 ttctatatac tttttagctg catccttcga attatcaggc aaatccagcc ggagaagcct
     9721 attgcatata tcattgtaga gcctgattac atattcctta ttcataaaat cctccttaag
     9781 cactggaaat tgcggaaact acagcatagt taaattatat tatattccaa ttaaatatat
     9841 ttttcaaact tcattagatt gtggtataat aatgcgaaaa cgaaggaaat tgcgacgggg
     9901 acatggggac gcggggaaac gtccccgcgt ccccgtatat gagacacggg ggacgtttct
     9961 cctgtctcac tgaaagcgag acacgcgaaa cgtccccatg tctcataaaa gtgtgcctcg
    10021 atatactata caagctcttg cttagtatgc atcctgcaga atactgccat atgtataaag
    10081 gaaatgcaaa taccgacgtg gggaagctat attcgacgaa accttccacc tgacctcgga
    10141 acggaaaggc ttccaggcaa aataaccaga cgggcttgca gagatgggac ccacgccagt
    10201 gattttgctg gctttggaag gtgaggagaa catacttccc ctacagtcgg agtactttac
    10261 aaacccttga ttataagtcg cattcttcct ataaggagat acagtaatgg gcaaaaagca
    10321 gagaattgac aagatactat ccaatatggg gtatggcacc agaaaagaag taaaagaact
    10381 gatttcgggc gggcttgtcg aaatagacgg gattgttgtg aaggagccgg ggcagcaggt
    10441 gacggctgat tctcaggaaa taaaggtagg gggccgtaag ctcttgtaca aggatttcat
    10501 atatataatg atgaacaagc cccaaggagt catatccgcc acggtcgaca gcagggagaa
    10561 gacggttatt gacttgctgc cggacgaatt caaggccttt aagccttttc ccgtgggaag
    10621 gcttgacaaa gataccgaag gcctgctttt gcttaccaat gacggacaga tggcacacag
    10681 gctgctgtct ccgaagaagc atgttccgaa gacatacata gcaaaggttg cagggagggt
    10741 aagccaggag gatgcggaag ctttcggcaa gggggtaatc ctggaggacg ggtacaaaac
    10801 catggcatca aagcttcaaa tacttgtaag aggaagcatc tctcatgttg aggttactat
    10861 atatgagggc aagttccacc aagtcaaaag gatgtttcag gcagtcggca aggaagtgat
    10921 atttttgaag cgcatttcca tgggggagct gaagctggat gagactcttg aacccggaga
    10981 atacagggag ctgaatgaag aggaactgag aatactgtcc tgatttattt aaacttgagg
    11041 ttgcatgttt gttagattat tgataatata attaattaga tatatatcga attacttata
    11101 taataagctt tttttaaagg ggtgctgcaa atggcaatgg aaatatacgg agagtccgac
    11161 gagcctttag taaggattgt gaattctccg gttgtcgaac ttatatgcgc tgttcacctg
    11221 cttaccgacg ctgctcatca tcagtatgag ttaaggtggg cggaggaggt tctttccgga
    11281 ttcaagcagg aagagctgga ggctttgaat accattaagc tgttttcatc aggctggatg
    11341 aatatgttga atctaatact ggaaagcggc atacttacat caataccgga attcataggg
    11401 tacattgaac ggctcgattt gctggacttt ttttatttct tcttaaatga ggaagttgag
    11461 agaaccaaga ttcagaaggc atttgcatat aagaaggagc ggagcaggat acaggaaaaa
    11521 acaaagtggg cagtagaaga aagcgagctt atggaatttt ttgaatttcc tgagaagata
    11581 aaaagagata ctataaattt atttaaaaaa gtttatgctc atgattttat ggcaaagctg
    11641 gaccggtgca aggaaaaaat ggaagccggg atacagtatg tagaaagctt tacagggaaa
    11701 gacagcgtgg aatccgtgct tgaaaaaatc tccggaagat caaggaaatt gttcagcggg
    11761 tttaaagaat actatataat tccttcatat ttcatatcac cccagaaaag cctgagaata
    11821 tactccggtg ataggatata tgtggttttt gactgcagag tgaccaggga caagcgtgag
    11881 gctttattgg aagagctgtc ttcagtatta aagataattg atgataagtc gaggatggag
    11941 atattacgga tacttatgaa caataaaagc tacgggaagg ctctttccga cctggtgggc
    12001 ctgtcaactc ccactgtatc ccatcatttg gacattttaa agcaggccgg attaatcaag
    12061 gaagaaaaaa ttagaaatat caaatatttc tatgcggaca aggataaact aaaaaaagta
    12121 gtggatgata taaacaagta tttctttggg gcagagtagg gaaatcggct gtgcgaattt
    12181 gcacggccga ttatttattg taaaattttt tccgtgaaaa aggagatttc aagtttttgt
    12241 tgtatattaa attaatgtgc tatgcaattt tttgagatat tgcagaggga gtcgtgttta
    12301 tgaaaatcag agaaataacc gaaaacatgg aaaaaaataa cctctccaag tacgcctctt
    12361 taagtgtaaa ttccaaaggg aggcagactt gggaggaaaa atgcgatata agaaccgatt
    12421 atcagaggga tagggacagg atactgcatt cgaaggcatt caggaggctt aagcacaaga
    12481 cgcaggtatt catatctccc gaaggggatc attacaggac aaggctgacc catactctgg
    12541 aggtatccca gatagcgcgg accatagcaa gaggtctcag actgaatgaa gacctcaccg
    12601 aggcaatagc tctcggacac gacctggggc atacaccctt cggacatgcc ggagagaagg
    12661 tattgaatga aatacatccg gtcgggttca agcataatga acaaagcctc agggttgtag
    12721 atatacttga gggaggaaaa ggccttaacc tgacttatga ggtcagagac ggtattttga
    12781 ggcatacggg agatatcggg cctgaaacgc ttgaaggaaa tattgtcagc ttttccgacc
    12841 ggattgcata tataaaccat gacattgatg atgcacttag agggggtata ttggaaatgt
    12901 cctcacttcc cgagaagtgt attgcaatac ttggagatac ccacaagaaa aggattaata
    12961 caatgataac cgacataata acccaaagca tggataaaaa tgagattatg atgagcagta
    13021 tggtaaggca ggctactgat gagcttaggg aattcatgtt tgagaatgta tatatcggtt
    13081 cggaggccaa ggcggaagag aagaaaacca ggaatataat aagctgcttg tatgaatact
    13141 atgtgcaaaa ccctgaggaa atgcccattg agaatatcag ggagaatagg gatgcggata
    13201 atatagatat agaaagactt gcatgtgatt atatcgcagg aatgtccgac cgttttgcca
    13261 taaacaaatt taccgagctg tttattccat ccccctggcg agtatagcag ggaaataata
    13321 tccataaaat ttattttctt ttgaaggata ttaggattat ttgtcgaata aaaactatct
    13381 tgttacgtta attattaatt agaggtatat gtatgagaat attgatagac ggggacgcct
    13441 gccccgttac gcatttgatt gagaaaacag cagcacgatg tggggtcggt gttgtttttt
    13501 ttcacagcac atgtcattac agcaactatt caaatgcggc tgcggaaaaa ataatggttg
    13561 ataatgaatc acaggcagta gatatggcaa taattaataa agcagtaaag ggagatattg
    13621 tagtgacagg agactttgga cttgcatctc tgacccttgg gaagggagcg aaagctatat
    13681 ctttcagcgg tatgatattt gacgaatcca atattgaccg gcttcttttc gaaagacatg
    13741 tatccggagt aatcaggagg agcggcggca gaaccaaagg ccccgcaagg cgtacagaca
    13801 gtgacgacag gaactttgaa aaatcactca gctttctaat ttcacaaatt atgaaggata
    13861 tttaagataa atggagaact attatataaa taattaatga tatcatattt taccgtttga
    13921 ttaaagtaaa aggtggaaaa aaagatgttt ttctcagaag ataagatttc tgagataatt
    13981 gagaaaaacg atatagtgga tgttatttca gaatatatga cattaaagag gagcggcagc
    14041 aattatatgg ctttgtgccc tttccattcg gagaagacac cgtcctttag cgtgtcacca
    14101 tctaaacaga tatttaattg ttttggctgc ggtttcggag gcaacgtaat aacatttatc
    14161 cagaaaatcg aaagaattga atttgtggaa gcagtgaaaa tgcttgctga aaaagccggg
    14221 gtaaagctgg aagaggacaa ggatcccaaa cagttggaaa gagctaagca aaaaaaggca
    14281 ttgtacagga taaatgctga agctgcaagg tttttctaca gcaatctgaa tgccggatcc
    14341 aaagctttgg agtacctccg aagaagaggc ctgagtgatg caatcatcaa aaggttcggc
    14401 ttgggatatg caccggatga gtggtatggg ctgatgaacc atatgaaaag gaaggatatt
    14461 gataccgagc ttctttttaa ggcagggctt attgtcagga ataacgatgg tgagaaattt
    14521 cgcgacaagt tcagaaacag gataatgttt cccataatag atctcaaggg aaatgttatt
    14581 gcttttggag gaagggtaat agatgattca aagcccaagt atctcaactc acctgatacg
    14641 cttgttttta acaagggcta caatatttac ggtttgaatt ttgttaaaaa ggtgcagaac
    14701 ctggaaaata tagtagtagt cgaaggctac atggatgcaa ttgctttgca tcaatttgga
    14761 gtggagaatg ccgtcgcttc tttaggaaca gcctttactg aaaatcaagc taagcttttg
    14821 aagcgttttt caaatgaaat cataatagca tatgattcgg atctggcagg acaggctgcg
    14881 acattgaagg gattggcaat ccttgagaag gaaggctgtg tggtaaaggt actaagcctg
    14941 ccttcaggaa aggatcccga cgagtatata aggaaagaag gcattgatgc ttttagagac
    15001 agaataagga agagtgtttc ccttattgaa tatcagattg aaaatttaaa aagaggtttg
    15061 gacataaata acatacagga cagaataaaa tttacaaaag ggttctccga gattttgaag
    15121 aacgttgtaa gcaacgttga agtagatgcc tatatcagaa agtattccgg tattatgaag
    15181 gtaaatgagg aggctctgta tgcggagctg aacaggctca ggaaaaagaa taaaaatgga
    15241 aataataaac ataatatatt aaatagtata aaaaccgagc cggaattaaa aaaaggagaa
    15301 ataacagcag agatttatct tcttaatata tgtatttatt atttcaagaa agtaaaaaat
    15361 atttttgaga ttttgaaacc ggccgatttt tccaatgata tacatgtcag gattgcagaa
    15421 atcatccaaa gggcatataa ggaggataag gcaataactg caggtgaaat catcagttac
    15481 tttgataatg atgatgaaaa gtcaagagtc gcgggaatct tcagccaaaa actaccggat
    15541 tcggatgtgg acgggctgat taagtcaagc ttggataaga tcagacaatc gaaattggat
    15601 agaacgattc aggaaatgac agaaaaaatg aatgaaatgt atcacgcagg aaaaaaggaa
    15661 gaagccaata aaatctttgt tgagattaaa gagctgcaga agaagaagag gtaatatccg
    15721 gaagggaggg gatacggtga aaacagaaga acttagaatg acatatgtaa aagaattggt
    15781 tgataaagga aagaaaaacg gatcgctgac atataaggaa ataatggaca cttttgaaga
    15841 agtggaaatg gatcctgaac agatagataa gctgtatgaa tatcttgaga ctaaaggcat
    15901 agatgtggta ggagcaatag ataatgatat ggatattccg cctcttgatg aggatgtgga
    15961 ggaagtcgat cttgatataa gcattccgga aggtattagt atagatgatc ctgtcagaat
    16021 gtatctgaag gaaataggga aagtgcccct gctgagcccc gaggaagaga tcgatttggc
    16081 aaatagaata aagaaaaatg attccgaagc caaaaagaaa cttaccgaag caaaccttag
    16141 gctggtagtc agcattgcaa aacgatatgt aggaagagga atgctgttcc tggatcttat
    16201 acaggaaggt aatctggggc ttattaaggc tgttgaaaag tttgactata caaagggatt
    16261 taaattcagt acttacgcta cctggtggat tagacaggca attaccagag ctattgccga
    16321 ccaggcaagg acaatcagaa tacccgtgca tatggttgaa acaataaata agcttataag
    16381 ggtttcaaga cagctcctgc agcagcttgg cagagatcct atccccgagg agattgccgc
    16441 tgaaatggga atgtcggagg ataaggtaag ggagattatg aaaattgccc aagagcccgt
    16501 ttctctcgaa actcctattg gtgaagagga agacagccat ttgggggact tcatacctga
    16561 tgaagacgct cccgcaccgg ccgaatcggc agcatttaca ctgcttaagg agcagcttat
    16621 ggaggtgctt gatactttga ctccgaggga ggaaaaggtg ctgaagctga ggttcgggct
    16681 ggaggatgga agggccagaa ccttggagga agttggcaaa gagtttaatg tcaccagaga
    16741 gcgtataagg cagattgagg caaaagcatt gaggaagctc aggcatccaa gcagaagcaa
    16801 aaagctaaag gatttccttg aataaacaaa atttactgtt gactttcata ataaaagtta
    16861 gtaaaataat atttgtcagt ttaaagttcc tcaatagctc agtcggtaga gcatgcggct
    16921 gttaaccgca gggtcgttgg ttcgagtcca acttggggag ccattttcgg gcctttagct
    16981 cagttggtta gagcaaccgg ctcataaccg gttggtccgg ggttcgagtc cctgaaggcc
    17041 caccatttat tatgagtata aacctgatac gaaagtatca ggttttttac tatccaccta
    17101 tttgtttccc ccgccgggag gcttttttat ttcgctttcg caatcctgct tctcaataat
    17161 attattgatt gcttccatgg tttcaagctg gttggacagc ttctcggctt ctttctccca
    17221 ttccttggta gttcctcccc aactgaattt ttgtattacc tccggcttga tatgaagctt
    17281 cttactcatt tttttcactt cctttattgt atttttctca tttattaata aaaaaattta
    17341 taaagtgtag tataataaat aataataagg caagacacgg gaactgagca gggtgtcgaa
    17401 gcatgcagca gcaattatac attaataatg taaggatgat gatattgaat cttactccca
    17461 gactgaaaat tatagcagac agtatacaag gctttgagac attggcggat atcggttcgg
    17521 accacgcata tcttcctata tatttaataa agaacagacg ggtaaaaagc gttattgcca
    17581 cagatgtgaa cagcggtccg gcagagatat ccaggaaaag aatcaagaat catggactgg
    17641 aatcgaagat aagcgtgcgc gaaggaagcg gcctcagggt gataaattct ggggaagcag
    17701 aggtcattgt tattgcgggc atgggaggaa ttctcatagg agatattttg gataagggca
    17761 tagatgcggc aaaaagtgcc aagcttttga ttcttcagcc gatgagagat tcggaaaagg
    17821 taagggaatg gcttcttgaa catggttttg acataattga tgaggaactg gtaaaagatc
    17881 aggataaaat atacgaagtc atatggtcgc agcccggaga ggaagcgggg gaagcaaagg
    17941 ggccattgct tataggagaa aagattattg aaaaaaagca tcctctattg gaggaattta
    18001 ttaatagaaa agtaaaagaa ctggagaaaa taatgattgc cctggaagct atggattcgg
    18061 ataattgcag ggagagagcc gaagaatgca gggcaatgct ggcttattac agggaggtgg
    18121 ctgaatgggc acgataaaat gtcagactat tgcgggaatg ctggatgaac tggcacccag
    18181 aagccttgct gaaagctggg ataacgtggg cctgcttgta ggcgacggaa ggaagcgcgt
    18241 cagccgtatc atggtttgcc ttgatgttac ggaatgggta atagaagaag ccgtagataa
    18301 taatgttgat atgataatat cccatcatcc catgatattc agcggaataa aaaaagttaa
    18361 tacggatact gccacaggca ggaagcttat tagccttgtt aagaacgata tatccctgta
    18421 ctgtcttcat accaattttg atatcgtgtc aggggggctg aacgatattt ttgcaaaaga
    18481 actcggattt tcgcattgga caattattga agagacatac agggaaaagc ttttcaagtt
    18541 ggttgtctat gtacctgcgg ggcaagagga cagtgttgcc gatgcaatgt gccgtgcagg
    18601 cgcgggttat ataggcaaat acagcggctg cactttcaga agcaaaggga tcggcacctt
    18661 tgagcctcaa gagggggcaa aaccttttct cggcagcata ggaaaccttg agagagttga
    18721 ggaattcagg gtggagacaa tagttcccga gaagcttttg agcaaagtgg ttggggagat
    18781 gttaaaggct cacccctatg aggaagcagc ctatgatata tatgaaacaa aaaatgaagg
    18841 ccgtgttatg gggcttggga gaatagccga gttggaaagg gaaacgactc tttccatgtt
    18901 tgccggaaat gtgaaaacct tgctgaatgc cgaaaacatt aggtatgcag gagatccgaa
    18961 caggagtatc aggaaggttg cgcttctaaa cggctcggga agcaaattca taaattctgc
    19021 aaggcttgcc ggagcggatg tgctgataac cggagacctg cagtatcata gcactcttga
    19081 agctctggaa atgggcttgt gtgtaattga tgcagggcat ttcagcacag agaagataat
    19141 gatgaagtat attactgaat ttttaaagaa caaattcaga gagcttaagg ttgaagcgga
    19201 tgtttttgaa tcatgctcaa atacggaaat tttgagagta ttataggaat agtcagctat
    19261 tcctattttt atttttttaa aaggaaaaag gagatggtct gatgaatttg ccattgcttt
    19321 atgaactgca gcatctgaat aaggaaatgg gtgcaatcac caggaagctc agagagcttc
    19381 aggaagacaa ggacatgaaa aagtataagg aggagtatca aaggctcaaa gaggaatacc
    19441 ttaaaggcga agaaaaattg aagaaaaatg cttaccggca ggaagcaagg aataatgaga
    19501 ttagaaacat agagtacaac atgaaatcat cgttagaaat caagttcagc aaagaaacaa
    19561 acacagtcaa aaaattggag aacattgaga aacaacttga aaagcttgaa gaaaaaaagc
    19621 aggcggcaga aaatgaaata atagaactca ttaatgatgc ggataatatt aataaagaac
    19681 ttgctgagat aaaaaagaaa ctggcattta ttaaaaagaa atttttgaat tgcaaagagg
    19741 atgccgacaa ggcggctgcc gggttgaaag cacaaaaatc agagcttcag gccaaaattg
    19801 acagcatgat gaaatccatt gatcaagaaa gtcttgaaat gtacaacaga ctgataaaaa
    19861 cacattccga tccggttgcc cttgttaaaa accgcatttg cagcgggtgc aagatggaag
    19921 ttcccgctat tgattatgaa gcgctgaaag gcggcaatca agagctgaaa tgccaaagct
    19981 gcggaaggat tttattttac aaaaaatcct gatgctaagt aaagatatgg cataacatct
    20041 taatatatca tattgactga atctcctttc ggatatataa taaatatct
//
LOCUS       DAOOAB010000047        20074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3558_length_20074_cov_15.047105, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000047 DAOOAB010000000
VERSION     DAOOAB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3558_length_20074_cov_15.047105"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1855)
                     /gene="ftsH"
                     /locus_tag="PKU88_09705"
     CDS             complement(38..1855)
                     /gene="ftsH"
                     /locus_tag="PKU88_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPX69587.1"
                     /translation="MKEQNNPRKPIIYFYIVTLVIVMLLNAYIFPAVVKQQVTQVDYG
                     TFLTRIDEGKVKSVEIQDNQIAFTAIDEAGKENIYVTGKVNDPELVDRLLAADIEDFS
                     QVIPRENSPLMNFIISWVLPLIFFIGMGQLLAGSMQKRMGSNAMTFGKSNAKIYVEAQ
                     TGKTFDDVAGQDEAKEALSEIVDFLHNPAKYKEIGAIMPKGALLVGPPGTGKTLLAKA
                     IAGESKVPFFSISGSEFVEMFVGMGAARVRDLFRQAQEKAPCIVFIDEIDTIGKKRDS
                     GGIASNDEREQTLNQLLAEMDGFSSEKGVVILAATNRPDSLDKALLRPGRFDRRVPVE
                     LPDLAGREAILKVHAKKIKLGNDINFNEIARATPGASGAELANIINEAALRAVKCGRK
                     YVKQIDMEEAVEVVIAGYQKKGAVISPKEKEIIAYHEIGHAIVAAKQTESAPVHKITI
                     IPRTSGALGYTMQVAENENVLMTKEEALNKIATYTGGRAAEELVFGTYTSGAANDIEQ
                     ATKIARAMVTRLGMSKAFDMMGLETISNVYLGGDPSMTCSSETASKIDEEVLAIIKSA
                     HEKAINILKENMEKLHLLSSYLIEKETITGEEFMRILES"
     gene            complement(1891..2337)
                     /locus_tag="PKU88_09710"
     CDS             complement(1891..2337)
                     /locus_tag="PKU88_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPX69588.1"
                     /translation="MEVAEFKNIVFDYTRKINESLHCAFGPAIGNRGLTMMQTRILME
                     LHRCESHTIGSLADSICAAGANISAMCKKLEEQGFIERIRNRKDERVVRVVLTRLGKE
                     TVLEIDELFNERISKCLKYESEEAFEDIIRGMQKLNELLTRIIKNE"
     gene            complement(2489..3418)
                     /gene="rarD"
                     /locus_tag="PKU88_09715"
     CDS             complement(2489..3418)
                     /gene="rarD"
                     /locus_tag="PKU88_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPX69589.1"
                     /translation="MDGKKEGLQVKNDVSGVVYGVIAYTVWGLLPIYWKLLGEIPADE
                     ILAHRIFWAFLFVGGLFLSKNDIGVLKEAIKERKNIRNILICSFLITINWGTYIWAVN
                     SGRILESSMGYYINPLMVVLLGMTVLKEKLNVLQYISIVFAAAGVIIITVQFGNIPWV
                     SLLLALSFALYGLFKKLLKVESLTGMVLETAAIMPLALGYILFKLFSGQSALYSVPLS
                     TAAILMLSGIATALPLLWFAMGAARLKLSTLGFLQYISPTLSLLLGVFLYGEKFTVTH
                     LLSFSFIWTGLIIYSFSNFEAKSKFGNSKQADV"
     gene            complement(3691..3816)
                     /locus_tag="PKU88_09720"
     CDS             complement(3691..3816)
                     /locus_tag="PKU88_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69590.1"
                     /translation="MENIFLSGQWLQPPGGLLVALITGKDTIMRLCKMKNKLFVY"
     gene            complement(3854..4267)
                     /locus_tag="PKU88_09725"
     CDS             complement(3854..4267)
                     /locus_tag="PKU88_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69591.1"
                     /translation="MNIEGNRVRRVICSSGKAFEAGYYIAACDAHVLYEKLLKGKYPD
                     PEFQKRFNNPEDYPLASEIRIAIGYEGRTDEIPRTLRFPIDSFKVNGRPINYLTITHY
                     GYEPDFAPEGHVLITCSINQFHADYDAWNALAAHH"
     gene            complement(4278..4784)
                     /locus_tag="PKU88_09730"
     CDS             complement(4278..4784)
                     /locus_tag="PKU88_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPX69592.1"
                     /translation="MDKIVIIGGGIAGLSAGIFAQKNGFESVILEKHNVLGGECTGWN
                     RRGYHIDGCIHWLVGTKKGTPIHDLWTAAGALDGVEIYHPESFLAFEYDGIRVHLYRD
                     LDRLKSSWLELSPQDEAAINNFCCTIKQLQSFEIPVGKPEDMMEFMEKIKLMISLFIP
                     GRRYRSPL"
     gene            complement(4988..5551)
                     /locus_tag="PKU88_09735"
     CDS             complement(4988..5551)
                     /locus_tag="PKU88_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX69593.1"
                     /translation="MGPKIKFTREQIIDAAFEIARAEGIDNVTMRKIAHRMGSSVAPI
                     YVNFKSINELIEALIEKIADIGQQLIAEENTGSPFADIGRASIRFAMEYSVLFRDLVM
                     KNSRYLKGYDEKVMPALIEEMKKDSDLEGFSEDKLKEILLKMRIFQLGLSVMAASGIL
                     SKDYDMKDLTDLLSSTADDVILSARLK"
     gene            complement(5917..10860)
                     /locus_tag="PKU88_09740"
     CDS             complement(5917..10860)
                     /locus_tag="PKU88_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HPX69594.1"
                     /translation="MKNNNTIQLLVKTIAITAMFLLAAFLLIPDTVLAEGGFARGDGS
                     PEQPFEIATPEQLATVAEEVYCSSHFKLIDHIDLSDYLSDGGGGWNEGAGWKPIGTYD
                     MFDPTKAFTGSFDGDGYTISSLFINRSDTEGVGLFGYTVGATIKDVTLSDADITGDAC
                     VGSLVGVNENCTISGCSAENAEVTGFTTVGGLVGSNDSGNIANSLATAEVSGVFSVGG
                     LVGDNYGGSIESSYATGTVSGIDYDDPESQGHFGGLAGRNYIYGTIENSYSTGNVAGI
                     HLVGGLVGSNTDAGVIENSYSTGDAAGTAYVGGLVGLNSLDEESYDNPFITDCYAAGS
                     VEGEQYVGGLVGQNCEGDIVHSFSIGEVSGTNKVGGLVGENEDGTVTDSFYDSNTSGQ
                     SDEGKGERKSTAEMNAYTTFFNASWDFIGEMDNGNEEIWGIDESADNPANDGYPFLTW
                     QGYGHKVWPAVSTMEAEDITDITAVGQGGIIIIGYPNPTQHGVVWSSHEDPTLEDNKT
                     EKGAVLGAGSFTSEITGLEPETTYYVRAYATNDLGTVYGEQISFTTEEGTFAGGNGLP
                     ENPYQIQTADQLAAVSEYMDSYFVLVDNIDLSAYGDGYDGGKGWLPIGNYDNSFTGSF
                     DGNGHTITGLYINRPDTYYVGIFGCTGADAEISNLRLLNVDVSGYGYVGALVGENHGT
                     ITGSCAAGSVEGSDYVGGLVGLNWEATGNIIDSYSAVNVVGAESVGGLAGGNEGTITG
                     SHATGDVVGEDYIGGLTGGSGTSDYRCSITDSYATGNVTGNGIVGYVGGLTGQNFNAD
                     IENSYAVGTVTGSNNVGGLVGINEADGTVTSSYYDTETTGQTDGGKGVPKTTAEMKQQ
                     GSFVGWDFTDTWNLAGGTYPYLEWQTDNFDFAPPVFTVGYPKAEDITYERMDILVQVD
                     EVAAAYYVVLANGVDAPDAAQVKAGMDSENTVLAENLKGSIAGISAGTDGRAVITGLA
                     PGTEYDIYVVAEDNAGNMQPDGSVALLEACTGLMPTGNMSFALTTPVPEADGNSIAVD
                     QSSPKIVDVDTVNGIGSVEITGTKTAAQSVTVGGVDADKVTAAGEATEPVYTIDTSAI
                     AAEGGTYEFTLTVSEAGKADIVYNVTVTVAVPDNTPPVPGNGGTITVSNITHNSLELS
                     WAAASDDVTAQQNLQYKVVRSESNNIDTAINAESNGTIVKDWTAGLTGTEASGLSAET
                     IYYFNVIVQDEAGNMAVYTMKSQAAGSVPEREDEDDRDEDDRSERNVSGNSPVYNAVI
                     KGSNTSGTTITVNVNTRRGSAVVNLGEQFEDVSNGEEVPLITIPSIPGVNAYTVGIPA
                     DSLSGSQSKDILTFSTPEGSITLPGNMLAGIAQTGSKGAGITVGEGDKSLLPDDVKAA
                     LGDKPLVQLSLTLDGNQAEWNNPDAPVTVSIPYKPTAAELSDPEHITVWYIDGSGNVV
                     EVPSGRYNPETGMVTFSTTHFSHYAVAYVTKSFNDLGSAAWAKKPIEALASKGILKGI
                     SETEYAPQTNITRADFLYSLVRALGIYAKVEGNFDDISRDAYYYREIGTAKKLGITGG
                     TGNNKFSPDESITRQDMMVLTERALRMLKKLEVQSSASDLDRFADKTLIAAYATDSIA
                     SVVREELIVGSGDRINPLGSTTRAEAAVFLYRIYNRF"
     gene            complement(10894..11127)
                     /locus_tag="PKU88_09745"
     CDS             complement(10894..11127)
                     /locus_tag="PKU88_09745"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPX69595.1"
                     /translation="MPKIIRVEPKDDHTLLIELNNKHMIVYDMKPRLETVRFCGLADL
                     NRFKDVRIEDENTLVWDSLCQITIDEVLSMIER"
     gene            complement(11235..13715)
                     /locus_tag="PKU88_09750"
     CDS             complement(11235..13715)
                     /locus_tag="PKU88_09750"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX69596.1"
                     /translation="MSMIHRVLQRKHINEQLKSVFAYPLTVAVAAMGYGKTTAVREFL
                     DRANAKYVWLSVESDYRSAGHIWDSLTRQLAKLRPDLGNQLNALGFPADASQRDKIIS
                     IIEEHTYLNDIVLVLDDYHYARSQELDKLLERIVRTNIEGFHIIIISRTKPEINIEEL
                     SLKGYCFMIASELFELSRDEIREFFKLYGCRIPDNSVRTVHEISEGWITAVYLILHRY
                     SATGRLETGRNIESLIESAIMSRYTEEESQILKSICILDSFTLQQAVYVTGNAAAAGI
                     IERMSAGNSFLRYDEQLDVYKMHNIFSEYLKKQIEKQGSSIEAEALYKRSGQWYIRSG
                     DILSGLSFLLKAKEYDLMLTEFERHGATQELDKDPQLIVEVFSQIPDEVKYRHPMSYL
                     TYAYNYISSVDMEGGAELLAQIEEHYRYDESIPDELRRRIAGEIELAGSFLYFNDLEK
                     MNEKYLNAYSLLDGKSSIANKDMIFTFGTPHTLYLYYREKGRLLWIVEYLDNYFHYFC
                     ELSKGCGTGFEELARAEYCLETANFNQAELYAFKSIYKAKPMEQTSLIICASLTLARA
                     YAAQGKFDKARELLDNLAAETAQYNNPVYNSTLDLCKGYLGGITCRLQDFAPWLESGD
                     MEQSDILFLGMGFNYIVHAQAVLLQKNYIKLEVLCEGMLQIFSMFNNLLGFLHTHILD
                     AAAKYNLYGIERAKAAMLQAADIGRADDIILPFAEYGAHILDILKALDNDEKGDEYIS
                     RLISAAEQYSTGLERFKEEKISVPSLTKREREVLKLVAEGKTNREIGAELFIAEVTVS
                     KNITSIYQKLGVTGRPSAVKKAIELNIL"
     gene            complement(13734..14324)
                     /locus_tag="PKU88_09755"
     CDS             complement(13734..14324)
                     /locus_tag="PKU88_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX69597.1"
                     /translation="MARLLFQGHGSFRIISDSDAVIYLDPYAGAGYEEPADVILVTHQ
                     HGDHNQIQLPARKSTCVVIQEKDALQNGEYKSFDIKGVKIRAVPAYNKNHDRSKCVGY
                     ILTVDGIKVYASGDTSTTEEMKEYPSHHIDYALLPIDGVYNMNPVEASKCADLIGAKH
                     TIPIHMAPGMLFDRHKAEQFKGKNRLIVSAGDEIEL"
     gene            14639..16555
                     /locus_tag="PKU88_09760"
     CDS             14639..16555
                     /locus_tag="PKU88_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPX69598.1"
                     /translation="MKNYKSYIIYLLIISLISLIIVIEYNSFKNNKGVPGIELKAKQG
                     ILDLTDLHFDDESLHSLDGEWSFFYNQFIMPDNYDYSAPGIITVPGLWNSYQYNKEEI
                     GASGYGTYRLKVLLPSNCNEKLALKIPDMSSSYRFFVNGKEISHNGVVGKSIEEETPQ
                     WKPLIKTLNTDSGQLDIVIHVSNFHHVKGGIWESIILGTEEAIYKHRELNLLLSFILI
                     GVLFISVFYYLVIFSVMQSNVTSLYLAMFSFMAGLRELLIREAVVFLIFPDISFKLMS
                     KLEYITVPSGPLLLALSLYSLYQTEFPRKIMSTTVTVFSLYIIVIIFTPLKIFGPLMH
                     LYLILFIIAFIYMIFVIAAAAKKKKPGAGTLLFGTLFLLLTALIDMGYVYKLHNNYDL
                     AYTFSIGLIIFILCQMHSLSLNIADVFQRAQGLAKAEMAFLQAQIVPHFLYNTLNTII
                     HLTRESPEKARSLLMELSKYLHGKFNFDLYNKNKLVTLEYELDIVKSYLAIESVRFSN
                     RLKVNYQIDEQTLNHKILPFLLQPLVENAVRHGFRKNSEDCIITISAFVQEKNLILSV
                     SDNGIGMPKNKILALTKCDYSQLGTGLTNINQRLNTAYGTDLSIVSTIDAGTTINIKI
                     PLRSDNVHDKSNIS"
     gene            16533..17276
                     /locus_tag="PKU88_09765"
     CDS             16533..17276
                     /locus_tag="PKU88_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPX69599.1"
                     /translation="MIKAILVDDELFALKELQLQLESLPNIQIIGSFTNPLESFFNIT
                     MLNPDVVFLDIDMPEVSGIYLAEQIMTTHPQVKIIFVTAFDKYAINAFELNAVDYILK
                     PFTLERIKKTISRLEEKIEKNINTDIHSLSNQYKESVKKFFIYEQDNIILLNIKDIYY
                     IEALNKFVRIRTKDRFYNSNHSLNYFENKLKNLDFFRTHRGYLVNLDKIAEIIPKINY
                     SFDIRMTDTNDLIPVSRTNIKLLKQLLEL"
     gene            complement(17342..>20074)
                     /locus_tag="PKU88_09770"
     CDS             complement(17342..>20074)
                     /locus_tag="PKU88_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HPX69600.1"
                     /translation="IGTGIGVTTNMTITGDDADNYTIAQPVLSANITAKGLTVINAAA
                     QDKVYDGTADADIINAELSGVVDGDDVTLGNHTVGTFAQATIGTDIGVTTNMTITGDD
                     AGNYTIAQPVLATANITAKELTVINAEAQDKVYDGTADADIINAELSGVVDGDSVTLE
                     NNDAGTFAQATVGTGIEVTTNMTITGDDAGNYTIAQPVLSADITAKELTIGGTFTAED
                     KIYDGNTTVVIDDSDLTLTGVVDGDSVVLNAAAAFADANAADYIVVSLTDASSISGED
                     SGNYSLSLLGAPTTTADITPPPSDSKAITDFSLEAFEPDIQAVIDEGAKTITMEVPFG
                     TDVTALVPTIEHTGADIIPASGVVQDFTNPVTYEVTAENNTTAVYTVTMSVALPSNEA
                     CLTNLVISSGILNPAFVPDIQAYTADVANSTADITVTSTAAHPGASIEVNMTAVVSGE
                     ASQAIALNVGVNTINIIVTAEDGTERIYEVTVNRASSDSDNDDDRDNDDDNDRNTSIS
                     EPEPTTSSNITINVNGNSITSAASVDSETGTVTAVLNESAINNALTESQDDGQGVDTV
                     DIKIPKTTGANYYVTELPATVLTGNNTTRIEINSELGTITLPGNMLGDEGAYGIDNVG
                     LSIGTVDKSAIDEKVRTQIGDKPVIELKLKSKEGTISWNNPDAPVTVSIPYTPTEKEL
                     SDPEHITVWYIDGEGNVIEVPTGRYNPVTGMVTFSTTHFSNYAVVYVTKTFDDLENAA
                     WGKKSIEVLASKGVMKGVSEKEFEPQSNITRADFLYSLIRTLGVEARFDGNFDDISKD
                     DYYYKEIGIAQRMGITKGTGNNKFNPEESITRQDMMVLTERALRMFKRIEAQGAASDL
                     DKFADKSLIADYAVNSVASVVKEGLIVGSGDGVNPLGNTTRAETAVFLYRIYNK"
BASE COUNT         5336 a         4819 c         3704 g         6215 t
ORIGIN      
        1 gtccccgtgt cctcccccgt gtcctcttta ttcagcctta gctttccaat attctcataa
       61 actcctcacc tgttattgtt tctttttcaa tcaggtatga ggacaataag tgaagcttct
      121 ccatattttc ctttaaaata tttatcgctt tctcgtgagc gctcttaata attgccaata
      181 cctcttcgtc gattttggag gctgtttccg aagaacatgt catggaagga tcaccgccca
      241 gatatacatt ggatattgtc tccagtccca tcatatcaaa agctttgctc atgcccaagc
      301 gagtgaccat cgctcttgca atttttgtag cttgctcaat atcattggcc gctccggagg
      361 tataagtccc gaaaaccagt tcctccgcag cccgtccgcc ggtataggtt gcaattttat
      421 taagagcttc ttctttggtc ataagcacgt tttcgttttc ggctacctgc atggtataac
      481 caagggctcc cgaagtacgg ggtattattg taatcttgtg gaccggtgca gattcagtct
      541 gttttgcagc cacaatggca tgtcctattt cgtgataggc aataatctcc ttttccttcg
      601 gagatataac cgcacctttt ttttgatatc ctgcaattac tacttccacg gcttcttcca
      661 tatcgatctg ctttacgtat ttgcggccgc actttacagc tcttaaagcc gcctcattta
      721 ttatattggc aagctccgca ccggaggcac ccggagttgc ccttgctatt tcattaaaat
      781 tgatatcatt ccctaacttg attttttttg catgaacctt cagaattgcc tcacgtcctg
      841 caagatccgg cagttccacc ggaacacggc ggtcaaacct gccggggcgc agcaaagcct
      901 tgtcaaggga atccggtctg ttggttgccg caagtatcac cactcccttt tcgctgctga
      961 acccatccat ctcagccaac agttgattca atgtctgttc ccgctcatca ttgcttgcga
     1021 ttcctccgct gtcacgcttt tttccgatag tatcaatttc atcaataaac acaatacagg
     1081 gggccttttc ctgtgcttgc ctgaaaagat ctcgcaccct tgccgcaccc atacctacaa
     1141 acatctcaac aaattcggaa cctgaaattg agaaaaacgg tacctttgat tctcccgcaa
     1201 tagcctttgc aagaagtgtc ttaccggtac cgggaggtcc tacaagcaat gcaccctttg
     1261 gcatgatagc accgatttcc ttgtactttg ccggattatg caggaaatct actatttccg
     1321 acagagcttc ctttgcctca tcttggccgg ccacatcatc aaaggttttg cctgtctgcg
     1381 cttcaacata tatcttggca ttgctctttc caaaggtcat agcgttgctg cccattcttt
     1441 tctgcatact gcctgcaagc agctgtccca ttccaataaa gaaaatcaga gggaggaccc
     1501 aggatataat aaagttcatc agaggggaat tttcccttgg aatgacctgt gagaaatctt
     1561 caatatcagc tgcaagaagc cggtctacaa gctccgggtc atttaccttc ccggtaacat
     1621 atatattctc tttcccggct tcatcaattg cggtaaatgc aatttgatta tcctgaatct
     1681 ctacactttt tacttttccc tcgtcaatcc gggtcaaaaa tgttccgtag tccacctggg
     1741 taacctgctg tttcacaaca gccggaaaaa tatatgcatt cagcagcatc actattacca
     1801 atgtaacgat ataaaaataa ataatcggct ttctgggatt attctgttct ttcatgcttg
     1861 tcctctcctt aacatcagtt aaattcatcc tcattcattt tttattattc ttgtcagcag
     1921 ttcatttagt ttttgcatgc cccggataat atcttcaaaa gcctcctccg attcatactt
     1981 caagcacttt gaaatccttt cattaaacaa ttcgtcaatt tccaatacag tctctttccc
     2041 cagccttgta agaaccacgc gaactaccct ttcatccttc cggtttctga tgcgctcaat
     2101 aaagccctgc tcctccaact ttttacacat tgcggaaata ttagcgccgg cagcacatat
     2161 actgtctgcc aggcttccta ttgtgtggga ttcgcagcga tgcagctcca tgagaattct
     2221 tgtctgcatc atggtcagcc ctctattccc aatggcaggg ccaaaggcgc aatgcaagct
     2281 ttcattgatc ttccttgtat aatcgaaaac aatgtttttg aattcagcta cttccatagt
     2341 gctcctccat atatataata ttatttatga tatataatat atatattgta tatataatat
     2401 tatatataac tataattgtc aaatataaat aaaaagggct ttcttttaca caaccaagaa
     2461 agccttctat gaaatgtatt tatggctctt atacatcagc ttgcttgctg ttcccaaact
     2521 tgctctttgc ttcaaaattg gaaaaggaat atattatgag gcctgtccaa ataaaagaaa
     2581 agcttaataa atgagtgacg gtaaacttct ctccataaag aaaaactcct aataaaaggc
     2641 ttaatgtggg agatatgtac tgcaggaaac ccaatgtcga aagcttcaac ctggccgctc
     2701 ccattgcaaa ccagagcagc ggcaaagctg ttgctattcc tgaaagcatc agtattgcag
     2761 cagttgataa gggtaccgaa tatagggcgc tttgcccgct gaaaagcttg aacaggatat
     2821 aacccaacgc caagggcata attgctgctg tttccaatac catacccgtt aaggattcaa
     2881 ccttgagaag cttcttaaat aagccataca atgcaaagct taatgccagc agaagtgaaa
     2941 cccaaggtat attcccaaac tgtacggtta ttataattac tcctgcagcc gcaaatacta
     3001 tggatatata ctgaagtaca ttgagctttt cttttagcac tgtcattccc agcagtacca
     3061 ccattaatgg attaatataa taacccatgc tggattccag aattctgcct gaattaactg
     3121 cccaaatata agtaccccag tttattgtta taaggaaaga acaaataagt atatttctta
     3181 tattttttct ttccttaatt gcttccttaa gtaccccaat atcatttttg gataaaaata
     3241 atcccccgac aaataaaaat gcccaaaata ttctatgggc taaaatctca tcagcaggga
     3301 tttcccccag cagcttccaa tatataggaa gcagccccca tactgtataa gcaattaccc
     3361 cgtataccac ccctgaaaca tcattcttaa cttgtaaacc ttccttttta ccgtccatag
     3421 cattacccct ttatattaat taagcataat gcagcctgtg agtacaatta tagagaaata
     3481 cctatcttaa atcaataggc cagagcttca ctttcaaaga taatgttctt atccattgcg
     3541 gaatatgatg gcaatcagta tgaatcaatg ttctcagatg ctcttcattt gcccgttcac
     3601 atagacaata ttcccttagt tcgtcatata aattattttt atacttatta tattcgttat
     3661 aatatataat cattttatac cacctgttct ttagtataca aacaacttgt ttttcatctt
     3721 acaaagcctc ataatagtat cctttccggt aatcagtgcc acaagcaatc ctccgggggg
     3781 ctgcagccac tggccgctta aaaatatatt ttccaaacct tttatacgcc ctgtatgagc
     3841 catcatcttt cctttaatgg tgggcagcaa gtgcattcca tgcatcataa tcagcatgga
     3901 attggttgat gctgcaggtg attaagacgt ggccttcagg ggcaaagtcc ggttcatagc
     3961 cgtaatgagt tattgtcaag taattaattg gtcttccatt aactttaaag gaatcaatcg
     4021 ggaatcgcag cgtgcgcgga atttcgtccg tcctgccttc ataacctatt gctatacgaa
     4081 tttccgaggc taagggataa tcttcaggat tattgaaccg cttctgaaat tcgggatcgg
     4141 gatactttcc tttcagcagt ttctcataca gcacgtgtgc atcacaggca gcaatgtaat
     4201 aaccagcttc aaaggctttg ccgctgctgc aaatgacgcg gcggacccta ttgccttcaa
     4261 tattcaattc aactgcctca cagggggctt ctatatcgcc gcccaggaat aaataacgaa
     4321 atcattaatt ttatcttttc cataaattcc atcatgtcct ccggttttcc caccggtatt
     4381 tcaaaagatt gcagctgctt tatggtgcag caaaaattat tgatagcagc ttcgtcttgg
     4441 ggtgataatt caagccagct ggatttcagc cggtcaagat cgcggtacag gtgcactctt
     4501 atgccatcgt attcaaaagc caggaagctt tccggatggt agatttcaac cccgtccaag
     4561 gcccctgcgg cagtccagag atcgtgaatg ggagtcccct tttttgtccc caccagccag
     4621 tgaatacatc cgtcgatgtg ataaccccga cggttccaac cggtgcattc accgcctaaa
     4681 acattgtgct tttccaaaat cacactctca aagccgtttt tttgtgcgaa tatgccggca
     4741 ctcaaccctg caatgccccc gccgataata actatcttat ccaatgctca ttttcccctc
     4801 tctattactc caggcaagct gaaatttttt ataaaacatt tattatataa cattagttat
     4861 ataataaatg ttacactgaa accgaaatta ttgcaagggg ttttttagaa aaatgcaata
     4921 aaaaatatcg ctccccttct gactgtaaaa atgccttagg gtgcgatatt aaagaattct
     4981 gatgttttta tttcagcctt gcggatagta ttacatcatc ggcagtgctt gacaataaat
     5041 cggtcaaatc tttcatatca taatcttttg atagtatacc gctggcggcc ataaccgaaa
     5101 gtcccagttg aaatattctc atctttaaca atatttcttt taacttatcc tctgaaaaac
     5161 cttccagatc tgaatccttc ttcatttcct caattaatgc gggcatgact ttctcatcat
     5221 agcctttcaa atatctgctg tttttcatta ccaaatctct gaaaaggaca ctatactcca
     5281 ttgcaaatct tatacttgcc cttccgatat cagcaaacgg gcttcccgta ttttcttcag
     5341 caataagctg ctggccgata tctgcaattt tttctatcaa agcttcaatc aattcattaa
     5401 tgctcttgaa atttacatat atgggagcca cagagcttcc catcctatgt gcaatttttc
     5461 tcatggttac attgtcaatt ccttctgccc tggctatttc aaaggctgca tcaattattt
     5521 gctcccttgt gaattttatc ttaggcccca tagtatcctc atccctttat tacaagattt
     5581 aacgaagtaa ggaaatagtt cattatataa catttgttat ataattatat tacattatta
     5641 attgaaatta tacaagtact acaattaaat aaggatatga aagactatgc cgtaaattgc
     5701 aaaagtaatt ttattcttga attcagacat gtgttattac ctcataattt agatttcaag
     5761 cattataagc caattataca atataaatta tttaatgttt actattattt tatcgcagca
     5821 caaaggtgcg gacaaagccg cacctttcta ttacactatc atatgaagaa atatccctct
     5881 gccctgaaca ttgttcctga agaggtgtgt cttatattag aacctattgt atatcctgta
     5941 caggaatacc gccgcctccg cccttgttgt gctgcccaga ggatttatcc tatcaccgct
     6001 gcctacaatc agttcttccc tgacaacgga ggctatgctg tctgtggcat atgccgctat
     6061 cagtgttttg tcggcaaacc tgtcaaggtc tgaagctgaa ctctgtactt caagcttctt
     6121 aagcattctc aatgctctct cagtcaatac catcatatcc tgccttgtga tactttcatc
     6181 agggctgaat ttgttgtttc ccgtaccacc ggtaatgccc agctttttgg ctgtaccgat
     6241 ctctctgtag taataggcat cgcggcttat atcatcaaaa tttccttcaa ccttggcata
     6301 aataccaagt gccctgacca gggagtacag gaagtccgcc cttgtgatgt ttgtctgagg
     6361 ggcatattct gtttctgata tacccttcaa tatccctttg gatgcaaggg cttcaatggg
     6421 cttctttgcc caggccgcgc ttcccagatc attaaagctt tttgttacat aggctacagc
     6481 gtaatgactg aaatgggttg tgctgaaggt aaccataccc gtttcgggat tatatcttcc
     6541 tgagggtact tccaccacat tgccgctgcc atctatatac catactgtga tatgctcggg
     6601 gtcagaaagc tctgctgctg tcggcttata tggtatggaa accgttacag gggcatcagg
     6661 attgttccac tccgcctgat ttccgtccag tgtcaatgag agctgcacca gcggcttgtc
     6721 gcccaaggct gcttttacat catcgggcag cagggatttg tcaccctcac ctacggtaat
     6781 accggctcct ttgcttccgg tttgtgcaat acccgccagc atgtttccgg gaagggtaat
     6841 gctgccttca ggagtggaga aggtcaggat gtctttactt tgagagccgg aaagggagtc
     6901 agctggaatt cctacggtgt aagcattaac tccgggaatg gaaggtatag tgatgagagg
     6961 aacttcttcg ccgtttgaaa catcctcgaa ctgttccccc agatttacta ctgcactgcc
     7021 tctccgggta ttgacattaa ctgtaatcgt ggtccccgag gtattgcttc ctttaatgac
     7081 agcattgtaa acaggcgaat ttccggaaac gtttctttca cttctatcat cttcatctct
     7141 atcgtcttca tcctcccttt caggaactga tccggcagcc tgacttttca tggtgtacac
     7201 agccatattg ccggcttcat cctgcacaat cacattgaaa taatagatgg tttccgcgct
     7261 taagccgctt gcctccgtac ccgtaagccc ggccgtccaa tccttaacga ttgttccgtt
     7321 tgattcggca ttaattgccg tatctatatt attactttcc gaacggacca ctttgtattg
     7381 caggttctgc tgtgcggtta catcgtccga tgccgctgcc caactcagtt caagactgtt
     7441 atgggtaata tttgaaacgg ttatggtccc gccgtttccc ggcaccggcg gggtattgtc
     7501 aggcacagct actgttaccg ttacattgta aacaatatca gctttgcctg cctcactcac
     7561 tgtgagggta aattcgtatg tgcctccttc tgccgcaata gcggaggtat ctatagtata
     7621 aaccggttct gtggcttctc cggctgccgt tactttatct gcatcaactc cgccgacagt
     7681 cacgctttga gccgctgttt tcgttccggt aatctccacg gaccctatgc cgtttacagt
     7741 gtccacatcc acaattttag gagaagactg gtcaactgca atgctgtttc cgtcagcctc
     7801 cggaaccggt gttgtcagag caaaactcat attgccggtc ggcataagcc cggtgcatgc
     7861 ttctaacaat gccacgctgc cgtcaggctg catatttccg gcattgtctt cagccaccac
     7921 gtagatatca tattccgttc ccggagccaa cccggtaatg actgcccttc cgtctgtccc
     7981 tgcggatatc cccgcaatgc ttcccttgag gttttcagct aagactgtat tttcagaatc
     8041 catgcctgcc ttaacctggg cagcatcagg tgcatctact ccattagcca ataccacgta
     8101 atatgcagcg gcaacctcat ccacctgcac cagtatgtcc attctttcat aggtgatgtc
     8161 ctctgccttg ggataaccca cagtaaatac aggcggagca aaatcaaaat tatctgtctg
     8221 ccattccaaa tacggataag tgcctccggc tagattccag gtatcagtaa aatcccaacc
     8281 tacaaaagaa ccctgctgct tcatttctgc agtggtcttg ggtacacctt tgcccccatc
     8341 ggtttgcccg gtggtttccg tatcatagta ggagcttgtg acagtaccgt ctgcctcatt
     8401 tattcctacc aacccgccga cattattgct tcccgttacc gttcccacag cataggaatt
     8461 ttcaatatca gcattaaagt tttgacccgt cagccctcca acatatccaa ctatgccgtt
     8521 accggtgaca ttacccgtag cataggagtc agtaatggaa cacctgtaat cactggtccc
     8581 actgcctcct gtcagtccgc ctatataatc ctcgcccaca acgtcccccg tggcatggga
     8641 accggttatg gtgccttcgt ttcctccggc caggccgccg acactctctg ccccaaccac
     8701 attaactgcg gaataagagt caatgatgtt gccggtagcc tcccagttca gtcccaccaa
     8761 gcctccgaca taatcactgc cctccacgct gcccgcagcg caggaaccgg ttatagtgcc
     8821 atgattttct cctaccagag cgccgacata cccatagccg gacacatcca cgttcagcag
     8881 cctaaggtta ctaatctcag catccgctcc cgtacagccg aatatcccca cgtaatatgt
     8941 atcaggacgg ttgatgtaga gtccggtgat ggtgtgtccg ttgccgtcaa aactgcctgt
     9001 aaaggaatta tcatagttcc cgatgggcag ccagcctttg ccgccatcat atccgtcccc
     9061 ataagcggaa aggtcaatat tatccaccag gacaaaataa ctgtccatgt actctgatac
     9121 cgcagccaac tgatccgcag tctgtatttg gtatggattc tcaggcaatc catttccgcc
     9181 ggcaaaagtt ccctcctctg ttgtaaaact gatttgttct ccgtaaactg ttcccaagtc
     9241 atttgtagcg tatgctctta catagtaagt agtttccggt tctaacccgg taatttccga
     9301 ggtaaatgat ccggctccca aaactgctcc tttttctgtt ttattatctt ccagtgtcgg
     9361 atcctcatgg ctgctccaga caaccccatg ctgtgtggga ttgggatagc caataataat
     9421 aattcccccc tgtccgacag cagttatatc tgtaatatcc tccgcttcca ttgtagatac
     9481 cgcaggccat actttatggc catagccctg ccaggtcagg aaggggtagc cgtcattggc
     9541 agggttatct gcagattcgt caatacccca aatttcttca tttccgttat ccatttcacc
     9601 tataaaatcc caagaggcat taaaaaatgt tgtatacgca ttcatttcag ccgtactttt
     9661 acgctcaccc ttgccttcat ccgattggcc ggaagtattg ctgtcgtaga aggaatcagt
     9721 aaccgtcccg tcttcatttt cccccaccag tccgccgact ttatttgtac cgcttacctc
     9781 ccctatggaa aaggagtgta caatatcgcc ttcgcaattc tggcccacca aaccgcctac
     9841 atattgttct ccttccacgg agcctgcagc atagcagtct gtaataaatg ggttatcata
     9901 agattcttca tcaagagagt taagccccac caatcctccg acatatgcag ttccggcggc
     9961 atcaccggta gagtaagagt tttcgataac tccggcatct gtattcgacc ccactaatcc
    10021 accgacaaga tgaatgccgg cgacgttacc ggtggagtaa gagttttcga tagtaccgta
    10081 aatatagtta cgtcctgcca aaccgccgaa gtgtccctga ctttcgggat catcataatc
    10141 aatacccgac acagtgcctg tggcatagga gctttcgatg gaaccgccat agttgtcccc
    10201 aaccaagccg ccaaccgaga aaaccccact aacttcagct gtggctaatg agttggcaat
    10261 attcccagaa tcattactgc ccacaaggcc tccgactgtg gtgaaaccgg ttacttccgc
    10321 attctctgcg gaacaaccgc tgattgtgca gttttcattt acccccacta gactgccaac
    10381 gcaagcatca ccggtaatat cggcatcact caatgtcaca tccttaatag ttgcaccaac
    10441 cgtgtaaccg aacaggccga caccctccgt atcactccta ttaataaaga ggctgcttat
    10501 ggtatatcca tcaccgtcaa agctaccggt gaaagccttg gttggatcga acatgtcata
    10561 cgtcccaatg ggcttccagc ctgcaccttc attccagccg ccacctccat cagatagata
    10621 atcgctcaaa tcaatatggt ctatcaattt gaaatgacta ctgcaatata cctcttccgc
    10681 aacagttgcc agctgctcgg gagttgcgat ttcaaatggc tgttccggcg aaccgtcccc
    10741 cctcgcgaac cctccttccg ccaaaaccgt gtccggaatc agcaggaatg ctgccagcag
    10801 aaacatcgct gtaattgcta ttgtcttcac cagcaattgg atggtattat tgtttttcaa
    10861 gtcattcact tcctttccaa taataattga ctatcatctt tcaatcatgc taaggacttc
    10921 atcaatagta atctggcaca ggctgtccca gacaagggtg ttttcatcct ctatcctgac
    10981 atctttgaag cggttaaggt cagcaaggcc gcaaaagcgc actgtttcca gcctgggctt
    11041 catgtcgtag acgatcatat gtttgttatt cagctcaata agcagtgtgt ggtcatcctt
    11101 cggttctacc cgtattatct tgggcatatc gacccctccc atctgcaaca aaaataataa
    11161 acccttgaat gatataatca tactagggtt taaaaaaatt tcatattatc agatcctata
    11221 cttttggggg aaaactataa aatattcagc tcaattgctt tttttacggc agagggcctg
    11281 cctgtgacac caagcttttg ataaatggaa gtaatatttt tactgacggt tacctcggct
    11341 ataaagagtt cagccccaat ttccctgttg gttttcccct ccgcaacaag cttcagcact
    11401 tcccgttccc gctttgtcag ggaaggaacg gatatttttt cttctttaaa cctttccagt
    11461 ccggtgctgt actgttcagc tgctgatatt agccgggaaa tatattcatc acctttctca
    11521 tcattatcca gggctttaag tatgtccaaa atatgcgcac catactctgc aaagggcagt
    11581 atgatatcat cagccctgcc gatgtccgct gcctgcagca tagctgcctt tgccctttca
    11641 ataccgtaca ggttatactt ggctgcagcg tccaaaatat gagtatgaag aaagcccaga
    11701 aggttgttaa acattgagaa tatctgcagc atcccctcac agagcacttc cagctttata
    11761 tagtttttct gcagaagcac tgcctgagca tggacgatat aattgaatcc catacccagg
    11821 aataatatat cgctttgctc catatcaccg gattccaacc agggagcaaa gtcttgaagc
    11881 cggcaggtta tccctcccag gtaacctttg cataaatcca gagtgctgtt ataaacagga
    11941 ttattatatt gagcggtctc cgctgccaaa ttatctaaca gttcccttgc cttatcaaat
    12001 ttcccctgtg cagcatacgc ccttgccaag gtcagactgg cacatattat taaggaggtc
    12061 tgttccatgg gcttcgcctt gtatatggac ttgaaggcat atagttctgc ctggttaaaa
    12121 ttggcggttt ccaaacaata ttccgccctt gccagttctt caaagcctgt accgcagccc
    12181 ttggacagtt cacaaaaata gtggaaataa ttatccaggt attcaactat ccacagcagt
    12241 ctcccctttt ccctgtagta tagataaagt gtgtgaggag tgccgaaggt aaatatcatg
    12301 tccttgttgg ctatggatga tttgccgtca agcaggctgt acgcattaag atacttttca
    12361 ttcatttttt ccaggtcatt gaagtacagg aagctcccgg caagctcaat ttcccctgca
    12421 atcctccgtc tcagctcatc tggaatgctt tcatcatacc ggtaatgctc ttctatttga
    12481 gccagcagtt cggcgccgcc ctccatatcc acgctgctaa tatagttata ggcataagtt
    12541 aaatagctca tcggatggcg gtactttacc tcatcgggaa tttgagaaaa tacctccact
    12601 ataagctgag ggtccttgtc cagttcctgg gttgccccat gcctttcaaa ttctgtcagc
    12661 ataaggtcgt attcctttgc ttttaacaaa aagcttaatc ccgacaatat atcgccgctc
    12721 ctgatgtacc actggccgct gcgcttgtat aatgcttccg cttcgatgct gctgccctgc
    12781 ttttcaattt gctttttcag gtattcactg aaaatattat gcattttata tacatccaac
    12841 tgttcatcat atcgaaggaa agagtttcct gcgctcatcc tttcaataat gcctgcagcc
    12901 gcagcattgc cggttacata cactgcctgc tgcaaggtaa agctgtcaag tatgcatatg
    12961 gatttaagaa tctgtgattc ttcctctgta tatcttgaca taatcgcaga ttcaatcaaa
    13021 ctttcaatat tccgtccggt ctccaatctg cctgtggcgg aatacctatg aaggataaga
    13081 taaactgccg taatccatcc ttcggaaatc tcatgaacag tccttacact attatcaggg
    13141 attctgcatc catacaactt gaaaaactct cttatttcat ctcttgaaag ctcgaacaat
    13201 tcactggcaa tcatgaagca gtatcccttg aggcttaatt cctcaatatt tatttccggt
    13261 tttgtccttg agatgataat gatgtgaaat ccctctatat ttgtccgtac tattctttcc
    13321 aacagcttgt ccaattcctg agagcgggca taatgatagt catcaagtac cagaacgatg
    13381 tcatttagat atgtatgttc ttcaatgata ctgattatct tgtctctttg agaagcatca
    13441 gcaggaaagc ccaaggcatt aagctgattg cccagatcag gccggagctt ggcaagctgc
    13501 ctggtaagag aatcccatat atgtccggca gatctgtaat cgctctccac cgatagccat
    13561 acatattttg cgtttgccct gtcgagaaat tcccttaccg ctgtggtctt tccataaccc
    13621 atggctgcca ctgccaccgt cagcggatat gcaaagacac ttttaagctg ttcattgata
    13681 tgcttacgct gcaaaacccg atgaatcata ctcatatcca cccccttgtc ttgctaaagc
    13741 tctatctcat cccctgcact gacaattaat cggttttttc ccttgaactg ctccgcttta
    13801 tgcctgtcaa agagcattcc cggtgccata tgtataggaa ttgtatgctt tgcaccaatt
    13861 agatcagcgc atttggatgc ttctacagga ttcatattat agactccgtc aattggaaga
    13921 agcgcataat caatatgatg tgaaggatat tccttcattt cttcagtagt ggacgtatca
    13981 cctgatgcat agacctttat accgtccact gtcaggatat aaccgacaca tttgctcctg
    14041 tcatggtttt tgttataggc gggtactgcc cggatcttta caccctttat gtcaaagctc
    14101 ttgtattccc cattctgcag tgcatctttc tcctgtataa caacacaagt gcttttcctt
    14161 gccggaagtt gaatctggtt atgatctcca tgctgatggg ttaccagaat tacatctgca
    14221 ggctcctcat acccggctcc ggcataaggg tctaaataaa ttaccgcatc actgtctgat
    14281 atgatacgga agctgccatg cccttgaaac aataatcttg ccatatttct accttcttat
    14341 ccaaaaatca tctcttggtt aatactacca taatgtggag atggtagcaa cagatgaccg
    14401 ctccgtaggc ggatatgctc tttattcgtt gtccgtaaac gagttttcca aactaataat
    14461 ttcagtaaat agtaaaaagc aactaatctt gcgattgtgg tttatatata gaaatattta
    14521 caatattatt ggataatttg ctctgcatat tatatcgaat tatgctaaaa tgtgagaaag
    14581 gcaagaattc agtgattaaa acaggcaaag tcaagattat tgataatagg tgaaggaaat
    14641 gaagaattat aaaagctata taatttatct attaataatt tcccttatat ctttaataat
    14701 tgttattgag tacaacagct tcaaaaataa caagggtgta ccgggaattg agcttaaagc
    14761 taaacaggga atacttgatt tgactgatct gcattttgat gatgaatcat tacattcact
    14821 tgatggtgaa tggtcatttt tctacaatca atttattatg cctgataatt atgactattc
    14881 agctcccgga attataactg ttcccggctt gtggaattcc taccaatata acaaagaaga
    14941 aatcggtgct tcaggatacg gtacatatag gcttaaggtc ttgcttcctt caaattgcaa
    15001 tgaaaaactg gctttaaaga tacctgacat gtccagcagc tatcgctttt ttgtaaacgg
    15061 aaaggaaata tcccacaatg gtgttgttgg taaatccatt gaagaggaaa cccctcaatg
    15121 gaagcctttg attaaaactt taaatactga ttccggccaa ctggatatag taattcatgt
    15181 ttctaacttc caccatgtaa aagggggcat atgggagtcc ataattcttg gcaccgaaga
    15241 agcgatttat aaacacaggg aattgaattt attactaagc tttattctga ttggtgtatt
    15301 atttatttca gttttttact atctggtaat tttttctgta atgcaaagca atgtgactag
    15361 cctgtattta gctatgtttt cttttatggc cggtcttaga gaactattaa tcagagaggc
    15421 tgtcgttttt ttgatatttc cggacatatc ctttaaacta atgtcaaagc tggaatatat
    15481 aaccgtaccc tcaggtccgc ttcttctggc attatcattg tacagcctgt atcagacaga
    15541 attccccagg aaaatcatga gtactacagt tactgttttt tcactttaca taatagttat
    15601 catttttaca ccgctgaaaa tttttggtcc tttgatgcac ttatatttaa ttttatttat
    15661 tatcgctttt atttatatga tttttgtaat agcagcagca gccaaaaaga aaaaacccgg
    15721 tgcaggcaca ctgcttttcg gcaccttgtt ccttctttta actgccttga ttgatatggg
    15781 ttacgtgtac aagctccata ataattatga cctggcatat accttttcta tcggacttat
    15841 tatatttatt ctttgtcaga tgcactcatt atccttgaat attgcggatg tatttcaacg
    15901 ggcacaaggg ctggccaagg cagaaatggc ttttttgcaa gcgcagattg tcccgcattt
    15961 tttatataac actctgaata caatcatcca tcttaccagg gaatcccctg aaaaggcccg
    16021 cagcttactt atggaactaa gcaaatattt gcatggtaaa tttaattttg acttatataa
    16081 taaaaacaaa cttgtcactc ttgaatacga gctggatatt gtaaagtctt atctggctat
    16141 tgagtcggta aggttcagca accgcttgaa agtaaattac cagatagacg aacagacatt
    16201 aaatcataaa atattaccgt ttcttcttca gcccttggtc gaaaatgctg taagacatgg
    16261 ttttagaaag aattctgaag attgcattat tactatttca gcttttgtcc aggaaaagaa
    16321 cctaatactc tcagtttctg ataatggtat aggaatgcca aagaataaaa tactggcatt
    16381 aaccaaatgt gactatagcc agcttggaac cggacttacc aatataaatc agcgtttaaa
    16441 tactgcctat ggaacagatt taagtattgt aagtaccatt gacgcaggca ccactattaa
    16501 tataaaaatt cccctaagga gtgataatgt ccatgataaa agcaatatta gttgatgatg
    16561 agttgttcgc gttaaaagaa cttcaactgc aattggaaag tctccctaac attcagataa
    16621 ttggttcatt taccaatccc ttggagagct tctttaatat aactatgctg aatcctgatg
    16681 tggtcttttt ggatattgac atgccggaag ttagtggtat ttacctggcg gagcaaatta
    16741 tgaccaccca ccctcaggta aaaatcatat ttgtcacagc ctttgataaa tatgccataa
    16801 atgcctttga gcttaatgct gtagattata tactcaagcc ctttaccttg gaacggatta
    16861 aaaagaccat ttccaggctg gaagaaaaaa tagaaaaaaa cattaatacc gatattcatt
    16921 ccttaagtaa tcaatataaa gaatctgtca agaaattctt tatatatgag caggataata
    16981 ttattctgtt aaacataaaa gacatttact atattgaggc acttaataaa tttgttcgta
    17041 tacgcaccaa ggacagattt tataattcca atcattcact taactatttt gagaataaat
    17101 taaaaaacct tgattttttc cgcacccata gaggctatct ggttaatctg gacaagattg
    17161 cagaaattat tccaaaaatt aattattcct ttgatataag aatgacagac acaaacgatt
    17221 tgattccggt tagcaggact aatattaagc ttctaaaaca gttactggag ctttgagtct
    17281 ttgtagaaaa aagcactgcg tttaataacg caatgctttt ttcttatagc tttaagttct
    17341 tctacttgtt atatattcta taaaggaata cagctgtctc agcccttgtg gtatttccaa
    17401 gaggatttac accatcaccg ctgcctacaa ttaatccttc ctttacaaca gaagcaacac
    17461 tgtttacagc ataatctgct ataagggatt tgtcagcaaa tttgtcaaga tccgaagctg
    17521 ctccctgtgc ttctatcctt ttaaacattc tcagcgctct ttctgtcaat accatcatat
    17581 cctgtctggt aatactttct tcaggattga atttgttatt gcctgttccc ttagtaatac
    17641 ccattctttg agctattcct atctctttgt agtagtaatc atccttactt atgtcatcga
    17701 aatttccatc gaatcttgct tctacaccca aagttcttat cagcgaatat aagaagtctg
    17761 ctcttgtaat atttgattga ggttcaaatt ctttttcgga tacacccttc ataactcctt
    17821 tggacgcaag tacctcaatt gactttttcc cccatgctgc attctctaag tcatcaaagg
    17881 tctttgtcac atataccact gcataattac tgaagtgggt tgtactgaag gttaccatac
    17941 ctgtgacagg gttatacctt cccgttggaa cttcaataac attaccttca ccgtcgatat
    18001 accataccgt aatgtgttcc gggtcagaaa gctccttctc ggtaggcgtg taaggtattg
    18061 atactgtaac aggtgcgtcc ggattattcc atgaaattgt gccttccttt gatttaagct
    18121 taagctcaat aacaggtttg tcacctatct gtgttcgaac tttttcgtca atagctgatt
    18181 tatcaaccgt tcctatactc aagcctacat tgtcaatacc ataagctccc tcatcgccca
    18241 gcatattgcc cggtaaggtt attgtcccca actcgctatt aatttctatc cgtgttgtat
    18301 tgttaccggt taatacagtt gcaggaagct ctgttacata atagttggct cctgttgttt
    18361 ttggtatctt aatatctact gtatcaactc cctgtccatc atcctgtgac tctgtcaatg
    18421 cattgttaat ggcactttca ttcaaaaccg ctgttacagt ccctgtttcg ctatccactg
    18481 aagcagctga ggttatggag tttccattaa catttattgt tatatttgaa gaagtagttg
    18541 gctccggttc agatatcgat gtatttctgt cgttgtcatc gtcattgtct ctgtcatcgt
    18601 cgttgtcact atctgatgat gctctgttca cagtgacttc atagattctt tctgttccat
    18661 cttcagccgt tacaattatg ttgattgtat taactcctac atttagagca attgcctgag
    18721 aagcttctcc gctaactact gccgtcatgt taacctctat tgatgctccc gggtgtgcag
    18781 ctgtggatgt aactgttata tcggctgtac tgttggccac atcggctgta tatgcttgta
    18841 tgtccggtac aaatgccgga ttgagaatgc cgctgctaat tactaagttg gtcaaacacg
    18901 cttcattact gggtaatgcc acactcattg tcactgtgta aactgcagta gtattattct
    18961 cagcagtaac ctcataggtc acagggttgg tgaaatcctg gactacacct gaagcaggaa
    19021 taatatcagc cccggtatgt tcaatagtag gtactaaggc tgtaacatcg gtaccgaatg
    19081 gtacttccat ggttatggtt tttgctcctt catctatgac agcctgtata tccggttcga
    19141 atgcttccag gctaaaatca gttatggctt tggaatcact cggtggcgga gtaatatcag
    19201 cagtggttgt gggtgcacct aagagggaca ggctgtagtt gcctgaatct tctccgctta
    19261 ttgatgaagc atcggtcagg ctgactacaa tataatctgc cgcattcgca tcggcaaagg
    19321 ctgctgcggc attcagtaca acgctatctc cgtcaacaac ccctgtcaat gtcaagtctg
    19381 aatcgtcaat tacaacagtt gtgtttccat catatatttt atcctcagcg gtaaaggtac
    19441 cgccgatagt cagttctttt gccgtaatat cggcactaag caccggctga gcaattgtat
    19501 agttgccggc atcatcaccg gttatagtca tgtttgtggt tacttcaata cctgttccaa
    19561 ccgtagcttg tgcaaaggtg ccggcatcat tattttccaa tgttacacta tcaccatcta
    19621 ctacaccgct aagctctgca tttataatat cagcatcagc agttccgtca tataccttgt
    19681 cctgtgcttc tgcattgatt accgtcaatt ctttggccgt gatgtttgca gttgcaagca
    19741 ccggctgagc aattgtatag ttgccggcat catcaccggt tatagtcatg tttgtggtta
    19801 ctccaatgtc tgttccaatt gtagcctgag caaaggtacc gacagtatga tttcccaatg
    19861 ttacatcatc accgtctact acaccgctaa gctctgcatt tataatatct gcatcagcag
    19921 ttccgtcata taccttgtcc tgtgctgctg cattgattac cgtcaatccc ttggcagtga
    19981 tattggcact aagtaccggc tgagcaattg tatagttatc ggcatcatca ccggttatag
    20041 tcatgtttgt ggttactcca atgcctgttc cgat
//
LOCUS       DAOOAB010000048        19931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3591_length_19931_cov_15.267408, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000048 DAOOAB010000000
VERSION     DAOOAB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19931
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3591_length_19931_cov_15.267408"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..965
                     /gene="spo0A"
                     /locus_tag="PKU88_09775"
     CDS             159..965
                     /gene="spo0A"
                     /locus_tag="PKU88_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HPX69601.1"
                     /translation="MNLQKIKILIADDNKDFCIILSEYLTAQPDFEVVGVAKDGLEVI
                     ELIESELPDVVILDIIMPHLDGLGVLEKLNSMQIEKVPKVIILSAVGQDKITQKALTL
                     GAEYYVVKPFDMDIFSKRIRECFGIANNETERKNYIHPVAVASPAVTTPRELESAITN
                     IIHEIGVPAHIKGYVYLREAITMVVNNMELLSAITKELYPSISRKFNTTPSRVERAIR
                     HAIEVAWGRGRIDVINNLFGYTIHNVKGKPTNSEFIAMIADKLRIELKIS"
     gene            1121..2140
                     /gene="steA"
                     /locus_tag="PKU88_09780"
     CDS             1121..2140
                     /gene="steA"
                     /locus_tag="PKU88_09780"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HPX69602.1"
                     /translation="MKLQGKIKAGARTKNLVNFLCPGDIAVIRHDDIDEMAALAIANT
                     GVKAVINTGKSMTGRFSSAGAAVLLENNIAVIDVDLSLEMFKDDDMITVLGNDIIIRN
                     RIYKNVCTAVNWKYIGKRRQESKENEKYEILKFIDNTINLTALEVDKFISVNNYPELS
                     TRLKGRHAVIVVRNSGTLDELNALKAYIKKFNPVFIGVDGGADLIINSGYIPDLLIGD
                     MDSVSDIGIYKSREIILHAYEDGHCPCLKRIGGMNVPYKIFAVRGTSEDAALMLAYNK
                     GAELIVLVGGHNCMYDFISKGRAGMASTFIVRTLIGERLVDCKGFGIISASENEGKDA
                     QWAKM"
     gene            2125..2799
                     /locus_tag="PKU88_09785"
     CDS             2125..2799
                     /locus_tag="PKU88_09785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX69603.1"
                     /translation="MGEDVSVIIPAYNESDCILRTLEGLKGIENIKEIIVIDDGSTDS
                     TYELLKPLRDIILLKNEYNEGKGSAVRHALDYATNRFVALLDADICESAAEIYKLIEH
                     INPCGKSIIIGRLPAPARKGGFGIVKRLSSGCFRAMTSRNVDSILSGQRVLPLDFLKS
                     IELPSGFGLEFKLTLEAVRRGYEVIEVPVDMRHRETGRNISGFMHRGKQCIDIIDIIR
                     NEMKRL"
     gene            2816..3655
                     /locus_tag="PKU88_09790"
     CDS             2816..3655
                     /locus_tag="PKU88_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl pentapeptide
                     phosphotransferase"
                     /protein_id="HPX69604.1"
                     /translation="MDLIVIAAVTLIIAHYLIRNIIKMLCRAESRICISSNYKGKRIP
                     SIGGIVFVPIQLAAVFQLLLQRPEHSYGYLSYMFLMLSMGFAGVIDDLAGDKRIKGLI
                     NHVRSTLKSNMTTGFIKALIGFLAACTISFRTTGSFAELAADVLVITLSANAINLFDM
                     RPGRAVKVFLAISLMILSSSAGRPAEALPLIILVMTVLIYMSYDLREICMLGDTGANI
                     LGISLGFYSTLFLSFSSKLMLLLLLILLNIAAERLSITALISKSRILSYLDSLGRRRT
                     DNR"
     gene            3652..4722
                     /locus_tag="PKU88_09795"
     CDS             3652..4722
                     /locus_tag="PKU88_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HPX69605.1"
                     /translation="MIDIFKAQVKEIIYRDVYISKFRIERNSMLCNCINYEMLTGSVE
                     VNDTVLLNTTAVQLGLGSGGYHYVLANLSKDSFSNIGLGHIMKLRYTPMQINCMAAEA
                     QESPYHKIFNDFESLRGLPVITGTLHSMLAPIALTLKHIAGKERIVYIMTDGGALPIW
                     MSETVKRLRRSGVLYGAITFGNAFGGDLECINVYTALIAAKEIMKADAAVITMGPGIA
                     GTGTRYGFSGIEQGYIIDAVNNLDGKAIAVPRISFADSRSRHYGLSHHTMMTLGKLCC
                     TKALIALPILEKSKDDLIRKQLEESGVLSRHRLIRLDGTEVEKLLETEKRQLERMGKS
                     FCQDKEYFITCGVSALLAMDSN"
     gene            complement(4715..4852)
                     /locus_tag="PKU88_09800"
     CDS             complement(4715..4852)
                     /locus_tag="PKU88_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69606.1"
                     /translation="MVKYVYKNGFHFVGKFNELMEQLNMIENKQITLREYILSYRYRL
                     N"
     gene            5008..5643
                     /gene="spoIIM"
                     /locus_tag="PKU88_09805"
     CDS             5008..5643
                     /gene="spoIIM"
                     /locus_tag="PKU88_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HPX69607.1"
                     /translation="MPDSLNDSIRRHLADNIWIYLIVMFLFILGISLGALAVNNIDEA
                     AKTDAITYLEGFLDLTNQNQLQSEAILKQSIKYNLYYALILFFSGVVYIGILVIPILV
                     AFRGFCIGFSIAFLTENVEGNGFLLSLGSVLPQNLIFVPVIIVMGVIGFNYSLWALRY
                     KCFKKSAAAPRLFAAYALTMLVLFILLVAGSIVEAYITSHIVKAIVPFIKY"
     gene            5737..6627
                     /gene="xerD"
                     /locus_tag="PKU88_09810"
     CDS             5737..6627
                     /gene="xerD"
                     /locus_tag="PKU88_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPX69608.1"
                     /translation="MILMSFIELFLDYLINNKELSANTLESYKRDIRQFEVYLREHAL
                     SLEKVTKTIIITYLIALQKAGKATSTISRNLASIRCFYQFMQNERYLDMDPSANLESP
                     KVEKKLPSVLTKKEVELLLEQPVPADAKGARDKAMLELLYATGIRVSELISLNIDDVD
                     LNSGLIICRNGEINSRSIPLGNVAVKYIKTYLNDFRKKLCTNENHKLLFVNFHGQKMT
                     RQGFWKIIKYYTAKANIKKSITPHTLRHSFAVHLIENGADLQAIQEMLGHSDISTTQV
                     YSKINKSRIKEVYSKTHPRA"
     gene            6704..7879
                     /locus_tag="PKU88_09815"
     CDS             6704..7879
                     /locus_tag="PKU88_09815"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HPX69609.1"
                     /translation="MVSRVILIVLDSVGIGELPDADMYGDSGSNTLGCLVRNVKDFKL
                     KNLEALGLGNIDRSIGLQIAENPTGSFGRAKEVSPGKDTTTGHWEIAGVTLSRPFPTF
                     PEGFPKEIVEAFERAIGVKTLGNEAASGTEIIARLGDKHVETGYPIIYTSADSVFQIA
                     AHEEVIPFQKLYEICEKARALLKDEYAVGRVIARPFLGKSGSYKRTSNRRDFSLKPIK
                     KTMLDIIVENGMNVAAVGKIEDIFAGEGISEAVHTKDNTDVVDKTLEYMSEDKTGLIF
                     SNLVDFDMLYGHRNDAEGYAGALMAFDERLPEIFAAMKDSDVLIITADHGCDPTTDST
                     DHSREYIPILVYGRHVRQGTDIGTREGFSDIGATVLDLLGLPIEIDGKSFKTEIYKI"
     gene            7898..8722
                     /locus_tag="PKU88_09820"
     CDS             7898..8722
                     /locus_tag="PKU88_09820"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPX69610.1"
                     /translation="MKENLLSLINEAGDYLTERAGAAPEAALILGSGLGDLAEEAENK
                     RIVGYKEIPGFPVSTVQGHKGRLVFGTMGGKRVVFMQGRFHYYEGYSMKEVVFPIWVF
                     NSLGVKKLIVTNAAGGINTSFKPGDLMIIKDHINYTNNNPLIGANIEAFGPRFPDMSA
                     AYSKELADIIRECAASEGIKLREGTYLYLTGPSYETPAEIRAFRILGADAVGMSTVPE
                     VLAANHCGIKTAGISCITNMAAGILDTPLNHKEVMETADRVKESFSKLIRGAMERI"
     gene            8747..9571
                     /locus_tag="PKU88_09825"
     CDS             8747..9571
                     /locus_tag="PKU88_09825"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPX69611.1"
                     /translation="MELRERIEKSTDFIRENTGFEPETGIVLGTGLGSLADYIDKEAV
                     IDYKGIPEFPVSTVLGHEGRLILGRLEGKKVIAMQGRFHYYEGYTMQEVTIPVRVMKQ
                     LGIKLLVASNACGGLNPNFAAGDIMIITDHINFMGTNPLIGPNLEEFGPRFPDMSQVY
                     DKELVELLEKIAASQGIRVHKGVYGAVSGPNYCTKAELSMMIKLGADTVGMSTVPETI
                     AARHCGLKVAGVSCITDMAIPDTMTAPSHEEIVKVAESVKPRFVSLVKQFIKEVRL"
     gene            9571..10872
                     /locus_tag="PKU88_09830"
     CDS             9571..10872
                     /locus_tag="PKU88_09830"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HPX69612.1"
                     /translation="MRMYDIITKKRNGEELTREEIQFFINGYTDGSIPDYQAAALLMA
                     VYFKGMSKRETAELTMAMVNSGETVDLSKIAGVKVDKHSTGGVGDKISLIVIPLAASL
                     GIPVAKMSGRGLGHTGGTIDKLESIPGFRTELTREEFIGNVNKYKMAIAGQSGNLVPA
                     DKKLYALRDVTATVDSIPLIASSIMSKKIASGAECIVLDVKAGSGAFMKSVEEAAELA
                     GTMVEIGKSLNRKTVAVVTDMSQPLGHEVGNSNEIIEAIDVLKGKGSEDERQVALTIA
                     SYMAVLGGAFEEFEKAHSALEEVIRSGKAIDNLKLLIQIQGGDPDVVDNTEKLPKAKY
                     HIEVRSKKSGYVDSIDAESIGISAMLLGAGRKTKEDKIDFSAGISMKKKVGDKVAVGD
                     ILCVLHTNLGNTAEAEKIALEAYGIGSTEKKIVKYIYEVIQ"
     gene            11001..12197
                     /locus_tag="PKU88_09835"
     CDS             11001..12197
                     /locus_tag="PKU88_09835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPX69613.1"
                     /translation="MYNSANINKLSLKLICYITIIAIAHFALNIPVCAENDMNLNCKS
                     AVLMDFGTGKIIYEQNSREKLPPASVTKVMTMLLIMEALDSGKISYEDKVTISPQASS
                     KNSKGTMLLLEAGEVRTVKELLYGIAVESANDACIAMAEHISGSEEEFVKLMNRRAKE
                     LGANDSNFVNPNGLPEDGHVTTAYDLAVLSRELLKHEKIFDFISQYMVKVYVGKKNNV
                     LRELVNKNKMVRFYNDVDGIKTGWTEEAGYCISVTAKRNNLRLISVVMGSPKVDIRNK
                     EARKLIDYGFANFNSLIIAKKGDKIQEVNVSKGSRLKVNALAESDVSVLLNKGEEKSI
                     VKKAELPEALKAPVKKGEKIGELVISINDKEIGRHNLISDADVNKSTFLNNLRNAIIY
                     WFGNSN"
     gene            12304..12960
                     /locus_tag="PKU88_09840"
     CDS             12304..12960
                     /locus_tag="PKU88_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPX69614.1"
                     /translation="MTDLLSKFLYYLNLNFGISSLLYSLPGIILALTIHEYCHALAAY
                     AMGDNTAKQYGRLTLNPIAHMDPLGFLCLIVTRRFGWAKPVPVNDMNFRNRRKGMLLV
                     SLAGPMSNFITAMLIAAAYAVFYKSMGEITKGILQIAYIINLSIGAFNLLPIPPLDGS
                     KILDAFLNDRQRFFMRRYAMYSNMLMLILIFSGIIGRVLGPVIMVMDWFINSVIGLFI
                     "
     gene            12985..13977
                     /gene="trpS"
                     /locus_tag="PKU88_09845"
     CDS             12985..13977
                     /gene="trpS"
                     /locus_tag="PKU88_09845"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPX69615.1"
                     /translation="MTRQRIMSGMRPTGTLHLGNYFGALENWVKLQNDYECFFSIVDW
                     HGLTTGYEDTSEFRNNTMEIAIDWLSAGLDPEKCTIFVQSNVKEIAELHLLLSMIVPL
                     SWLERCPTYKEQLSQLTEKNITTYGFLGYPNLMAADILVHKGNFVPVGEDQQPHLELA
                     REIARRFNYMYGDTFPEPQGKMTKIKVLPGLDGRKMSKSYDNTIAMSDSPETIREKIR
                     QMITDAEKIKKNDPGHPDICAVYAFHKVFSENTHQETYDVCKTGGIGCVQCKKVLAEN
                     VVQYMAPMYERRQKLLGNKEAILEILREGTRHAREEARETIKKVREKMKLMELE"
     gene            13981..14712
                     /locus_tag="PKU88_09850"
     CDS             13981..14712
                     /locus_tag="PKU88_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPX69616.1"
                     /translation="MAINVKLEAFEGPLDLLLHLVEKAEVDIYDIPIAEITDQYLHYI
                     NMMEIHDLEVSSEFIVMAATLLEIKSKMLLPKPKKEENITDEIDPREELVLKLIEYKK
                     YKEASLDLKDKLGIYSKVFYKPPEPIEDYIDDFASISNVSLDMLYMSFRNIILKNESR
                     RKNSYTEIYREVVTVEDKIRLINKLLAGKPSFYFDDLFIGCYNRYEIIVTFLAILELL
                     KRRSLLIEQEKNFSRILIKRRTKGA"
     gene            14705..15259
                     /gene="scpB"
                     /locus_tag="PKU88_09855"
     CDS             14705..15259
                     /gene="scpB"
                     /locus_tag="PKU88_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPX69617.1"
                     /translation="MHEGLGFVMDIREIKSIVEALLFVAGDAVPIKEICSIISIDETT
                     LRRIVMQMMDSFNEDNRGIQIIEVNNCYQICTKPEHYEYIERLVKPQSRQGLSQASLE
                     TLAIVAYKQPITKSQIDYIRGVKSDGCIARLAEKELIYEAGRLEGPGRAILYGTTDNF
                     LKLFGLRSIKELPALIELNGEEGA"
     gene            15456..16079
                     /locus_tag="PKU88_09860"
     CDS             15456..16079
                     /locus_tag="PKU88_09860"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HPX69618.1"
                     /translation="MLYAASGFIFLILILYLMPVEISTKVEKHNSNVKISLVLKTLYG
                     LFNVNTEIPAFKLDYENGKPVLKYKVEVANKERSRLLAGFSKLLTIEEGEGLYRIYKK
                     KKDIILPPLKYLTKKISVRNLYLKLKMGTGDAAATGLLYGTAWIAVGNIMALTRSYVN
                     IKEPRIIIAPIFDSARLSLDFSCIIRIKTGHIINAGIRVIPALITKL"
     gene            16095..16505
                     /gene="ytfJ"
                     /locus_tag="PKU88_09865"
     CDS             16095..16505
                     /gene="ytfJ"
                     /locus_tag="PKU88_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HPX69619.1"
                     /translation="MDQPVQSLMKTTMENIKNMIDVNTVVGDAVETKDGTVIVPVSKV
                     SFAFIAGGGEYTGATDKNEEDASKYPFAGGSGAGVTVQPIGFLATTNGQLRVIPVRYS
                     SAVDRIVDMVPDLINNMENYLKKKRHSEEDSLEM"
     gene            16620..17018
                     /locus_tag="PKU88_09870"
     CDS             16620..17018
                     /locus_tag="PKU88_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPX69620.1"
                     /translation="MELKSAVDMIWENRKYLTDDPKEVLSHLNEEVAESLKALLRGEE
                     DRAKSELEDALSCILIALKVMDVDIEEAVQRQIIQMKKRHDKLMIFKKDKVEIYVDGT
                     LKGGWTIWGEDDIKEALKIAKEFGCKIDKE"
     gene            17108..18241
                     /locus_tag="PKU88_09875"
     CDS             17108..18241
                     /locus_tag="PKU88_09875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPX69621.1"
                     /translation="MKRNTAIVMAAVIAALSGFFAVQALQESSARAAVVMDVNSNRIL
                     YSKNMDEKMAMASTTKIMTTLVAIESGRLDEKVTISKRASYMEGSSIYLREGEVHTVN
                     DLLYAIMLRSGNDAATAVAEHIGGSAEGFAEMMNKKAREIGANNTRFANPHGLDAEGH
                     YTTARDLALITSYALKNPLFSTIVSSKKKVMEGPPSENWDRVMINKNKMLWQFDGGDG
                     VKTGFTKKAGRCLVSSATREGMRLVCVVLNCGPMWDESSALLEYAFKNYVKKRIVDKD
                     SIFKVVEVRNGKERFVGVKPAEDFDLVLRTDRVENIKLEAKDLKAAQAPLKKGGHAGR
                     LEIYIDNNLLKTINLEYSEGVESSSPFYYLKRILTDYIADAKQ"
     gene            18381..19133
                     /locus_tag="PKU88_09880"
     CDS             18381..19133
                     /locus_tag="PKU88_09880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPX69622.1"
                     /translation="MAERLQKVMAEYGVASRRKSEEMICAGKVKVNGRLVTEPGFGID
                     KDRDVIEVDGDIIKSPEKKIYILLNKPTGYITSVKDQFGRPTVLDLLKGISTRVFPIG
                     RLDYDTEGLLILSNDGELTYRITHPKHSIDKTYRALVRGEADKSDIKIFERGITIEDY
                     VTAPAKMEIVRYSKGNSVIDITIHEGRNRQVRKMCSAIGHEVIRLRRIKIGDIGLGGL
                     KTGEWRYLRDSEIRYLKKLGGIWNDADKKNKT"
     gene            19105..19551
                     /locus_tag="PKU88_09885"
     CDS             19105..19551
                     /locus_tag="PKU88_09885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX69623.1"
                     /translation="MMQIRKIKPEDISFVIELASKYGFSDLKLLSNIEHFLICEDGNT
                     KCGCGCLVPYKGKGYLSCVIVDEGHRRKKLGGAITKALLNIADLQGIEEVYAAGICKD
                     FLQAMGFEKYNGTDLEVDLLEAIGDIGISEYYRVLLKDYFKPCSHI"
     gene            19670..>19931
                     /locus_tag="PKU88_09890"
     CDS             19670..>19931
                     /locus_tag="PKU88_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HPX69624.1"
                     /translation="MARIKITETSLRDGHQSLIATRMRTDEMLPIIELMDKVGYHSLE
                     MWGGATFDTCLRFLDEDPWERLRLIKSKLKNTKLQMLLRGQNI"
BASE COUNT         6426 a         3385 c         4878 g         5242 t
ORIGIN      
        1 ttctattgcg aaagtcgatt tttgtagaaa atggcataaa catgctgcat ttatttgtat
       61 gaaattgttt aacgattatt aggcaaataa tagcaggaaa tgaagctgct ttgtcgaata
      121 tgttttctat tgaataacaa tttatgggag gaataccaat gaatttacaa aaaataaaaa
      181 tcctgatagc tgatgataat aaggatttct gcattattct aagcgaatac cttactgcac
      241 agcctgattt tgaagtggtc ggagtagcca aggacggtct tgaagttatt gaactgatag
      301 aaagtgaatt gcctgatgtt gtaatattag atataataat gcctcattta gacggccttg
      361 gggtgctcga aaaactaaat tccatgcaga ttgaaaaagt tccgaaggta ataatccttt
      421 ctgctgtcgg tcaggataaa attacacaaa aagcacttac ccttggagca gaatactatg
      481 ttgtaaagcc gtttgatatg gatatcttct cgaagaggat aagggaatgc tttggaattg
      541 ccaataatga aacagaaagg aaaaactata tacatcccgt agctgtggcc tcgcctgcag
      601 ttactacacc cagggagctg gaatcggcta taacgaacat aattcatgaa atcggcgttc
      661 ctgctcatat caaaggatac gtatacttaa gggaagccat tacaatggtg gttaacaata
      721 tggaactctt aagcgctatt acaaaggaat tgtacccctc tatctcgagg aagttcaata
      781 ctacacccag cagggtcgaa agggccataa ggcatgccat cgaagtggcc tggggaagag
      841 gaaggataga tgttataaac aacctgttcg gctatacgat acataatgtg aaaggaaagc
      901 cgacaaacag tgaatttatt gctatgatag ccgataaatt gaggattgag ctgaagataa
      961 gctgaaggct tctaaaatta ccaagttaaa tactaaaatc actgaatagt ggttttagta
     1021 ttttttttgt gcaggaaagc atgctttaca aaataaacgc gtcgtaagac gcatttttta
     1081 ttttgttttg aaagatacta aaaacaatgg aggtgtggaa atgaagctgc aaggcaaaat
     1141 aaaagccggt gccagaacaa aaaaccttgt aaatttttta tgccccggag acattgccgt
     1201 catacgtcat gatgatattg atgaaatggc tgcccttgct attgcaaata caggagtaaa
     1261 agcagtcatc aatacaggga aaagcatgac gggaagattt tcatcagccg gtgcagcggt
     1321 actgctggag aataatattg ctgtcattga tgtggatttg tctctggaaa tgttcaagga
     1381 tgatgatatg ataactgtcc tggggaatga tattatcata cggaaccgta tttacaaaaa
     1441 tgtttgcacc gccgttaact ggaaatatat cggcaaaagg aggcaggagt caaaagaaaa
     1501 tgagaaatat gaaatactga agtttataga taatacgata aaccttacag ccttggaagt
     1561 tgacaagttt atttcagtta ataattaccc tgagctaagt acaaggctta aaggcaggca
     1621 tgcagtgatt gttgtaagaa acagcggtac tcttgatgaa ttaaatgcat tgaaggctta
     1681 tataaaaaaa tttaatcctg ttttcatagg ggtggacgga ggagccgatc ttataataaa
     1741 ttccggttat atacctgatt tgttgatcgg tgatatggac agcgtatcag atattggaat
     1801 atataaaagc agagaaatca tacttcatgc atatgaagac gggcattgcc cctgccttaa
     1861 aaggatcggc ggcatgaatg tgccctacaa gatatttgca gtcagaggga ccagtgagga
     1921 tgcagccttg atgctggctt acaacaaggg tgcggaactg attgttcttg tcggagggca
     1981 taattgcatg tatgatttca taagcaaggg aagagccgga atggccagta catttatagt
     2041 cagaaccttg ataggagaaa ggcttgttga ttgtaaaggg ttcggaataa tatcagcatc
     2101 cgaaaacgag gggaaggatg cgcaatgggc gaagatgtaa gcgtaatcat tccggcatac
     2161 aatgagtccg actgtattct tcggacgtta gaaggcctga aaggaataga aaacataaaa
     2221 gagataattg taatagatga cggttccaca gattctacat atgaactctt gaagcccctg
     2281 agggatatta tacttctgaa gaatgaatat aacgaaggaa agggcagtgc ggtaagacat
     2341 gccctggact atgcgaccaa taggtttgtt gctcttttag acgcggatat ttgtgaaagc
     2401 gctgcggaaa tatataagct tatcgaacat ataaatccct gcggaaaatc aataattata
     2461 ggccgcctgc ctgctcccgc aagaaaagga ggttttggaa tagtaaagcg gttatcctcc
     2521 ggctgcttca gggctatgac ttcccgaaat gtggattcga tattgagcgg acaaagggtt
     2581 cttcctctgg attttttaaa aagcattgaa ctgccttcgg gctttggact tgagtttaag
     2641 ctcaccctgg aagcagtaag aagaggatat gaagtgattg aggttcctgt tgatatgcgc
     2701 cacagggaaa cggggagaaa catttcgggc tttatgcaca gggggaagca gtgcatcgat
     2761 attattgaca ttattcgaaa tgagatgaag cgattgtgaa acagggtaga tatatatgga
     2821 tctgatagtt attgcggcag ttaccttaat aattgcacat taccttatcc gcaatattat
     2881 aaaaatgctg tgcagagcag aaagcaggat atgtatctct tcaaattaca aaggtaaaag
     2941 aataccttca atcggaggaa tagtatttgt tcccatccag ttggctgcgg ttttccagct
     3001 gctgctgcaa aggccggagc attcatacgg ctatctgagc tatatgtttc ttatgctgag
     3061 catgggtttt gcaggtgtca tagatgatct ggccggtgat aaaagaataa aaggcttgat
     3121 aaatcatgta aggagcactc ttaagagcaa tatgaccaca ggttttatca aggcgctgat
     3181 aggttttctt gcagcatgca caataagctt cagaacgacg ggctcatttg ccgaacttgc
     3241 tgcggatgtg cttgtaatca cactgtctgc aaatgccatt aacctttttg acatgcggcc
     3301 gggcagagca gtaaaggtct ttctggcaat atcccttatg attttgtcct cgtcagcagg
     3361 aaggccggcg gaagcgctgc cgttaataat acttgttatg actgttctta tttacatgag
     3421 ttacgattta agagaaatat gcatgctggg tgatacgggg gcaaatatac tgggaatttc
     3481 cctgggcttt tacagtacat tatttttgag cttcagcagt aagcttatgc ttcttttact
     3541 tcttatttta ctgaatattg ctgccgaaag gctgtctatt accgcgttaa taagcaaaag
     3601 caggatactc tcatatctgg acagcctggg aaggagacgg acagataacc gatgatagat
     3661 atattcaaag cacaagttaa ggaaatcata tatagggacg tgtacatatc aaaattcagg
     3721 atagaaagaa attccatgct gtgcaactgc attaactacg aaatgctgac aggcagtgtc
     3781 gaagtcaatg ataccgtttt gctgaataca acggcagtac agttggggct tggctccggc
     3841 ggttatcatt acgtactggc caatttaagc aaggacagct tttcaaatat cgggctcggg
     3901 catattatga aattgcgata tactcccatg caaataaatt gtatggcggc ggaagcccag
     3961 gaaagccctt atcataagat attcaatgat tttgaatcac tcaggggcct gccggtgata
     4021 acgggaacgc ttcacagcat gcttgccccc atagccctta ccttgaaaca cattgcgggg
     4081 aaagagagga ttgtctatat tatgacagac ggaggagcgc tgcccatatg gatgagcgag
     4141 acagtgaaaa ggcttcggcg cagcggggtg ctgtatgggg caattacctt cgggaatgca
     4201 ttcggtggag acttggaatg tataaatgta tatacggcac tgattgcagc aaaggaaata
     4261 atgaaggcag acgccgcagt aataaccatg ggccccggaa ttgcagggac gggcaccagg
     4321 tacggtttca gcggcattga gcaaggttat attattgatg ccgtcaacaa tcttgacggc
     4381 aaagccatag cagttcccag aatatctttt gccgacagca gaagcagaca ttacggcttg
     4441 agccatcaca ccatgatgac tttaggaaaa ctctgctgca ctaaggcact tatagcactg
     4501 ccgattcttg aaaaatcaaa ggatgattta ataagaaaac agcttgagga aagcggagta
     4561 ctgtccaggc accggttgat ccgcctggac ggtacagaag tagagaagct gctggagacg
     4621 gaaaaaagac agcttgagag gatggggaaa agcttttgcc aggacaagga gtactttatt
     4681 acttgcggtg tttccgcctt gcttgccatg gattctaatt aagcctgtat ctgtaagaca
     4741 aaatgtattc cctcaaggtg atttgcttat tttcaatcat attcagctgc tccatcagct
     4801 cattgaattt gcctacaaag tgaaaaccat ttttataaac atatttaacc attacattta
     4861 ccttccttgc atttgttaca tattatatgt gcaagtgtat attttcatta ctgtttgaaa
     4921 aaaatatgga atcataatat acttccttaa agcatattat caagatagag aaacagtgcg
     4981 gacaaaagct gagaggaggt ttttcttttg ccggacagcc ttaatgattc aatcagaaga
     5041 catcttgcag ataatatatg gatttactta atagtcatgt ttttattcat tcttggaata
     5101 agccttggag cattggctgt aaataatatc gatgaagcgg caaaaacaga tgcaataacc
     5161 tatctggaag gtttccttga tctgacgaac caaaatcagc ttcagtccga agccatattg
     5221 aagcagtcaa taaaatacaa cctttattat gcattgatac tgtttttttc aggggtagtg
     5281 tacattggca tacttgttat tccgatactt gtcgctttca ggggtttttg catcgggttt
     5341 tcaatagctt ttctcaccga gaatgtagaa ggtaatggat ttctcctgtc attgggttct
     5401 gtattgcctc agaatcttat atttgtgcct gtgattatcg tcatgggtgt aataggattt
     5461 aattattctc tgtgggctct caggtacaag tgttttaaga aatcggcagc tgccccccgc
     5521 ctgtttgccg cttatgccct tacgatgctg gtactgttca tactgctcgt cgcaggcagt
     5581 attgtggaag cgtacataac atcccatata gttaaggcaa ttgtcccatt tataaaatat
     5641 taaaaatatt aagaaagctt catgaaaaat tttcatttat tggaggattt ttattatctg
     5701 tgttgaatag ttaaaagata ctgtaataga ggggatatga ttcttatgag ctttattgag
     5761 ttgtttttgg attatctcat taataataag gaattatcgg caaatacgct ggaatcctat
     5821 aaaagagata taagacaatt cgaagtttat cttagagaac atgctctttc attggagaag
     5881 gttacaaaaa ccattatcat tacatatttg attgcattgc aaaaggcagg caaagcaact
     5941 tcaacgattt ccaggaatct tgcttccata aggtgttttt atcaatttat gcagaatgag
     6001 agatatttgg atatggatcc ttccgcaaat cttgagtcac caaaggtaga aaaaaagctt
     6061 ccctcagtgc ttaccaagaa ggaagtggag cttctgcttg agcagcctgt acctgctgat
     6121 gcaaagggtg caagggacaa agccatgctg gagctgcttt atgctaccgg catcagagtt
     6181 tcggaactta tttcattgaa tattgatgat gtagacctga attcaggatt aataatatgc
     6241 aggaacggcg aaataaattc cagatccata cctttaggca atgttgccgt taaatatata
     6301 aaaacttatc tgaatgattt cagaaagaag ctctgcacca atgaaaacca caagctgttg
     6361 tttgtgaatt tccacgggca gaaaatgacc cgacagggtt tttggaaaat cataaaatat
     6421 tatacggcaa aggcaaatat aaaaaagtca ataacacctc atactcttag gcattcattt
     6481 gcagtacatc ttatagaaaa cggcgccgac cttcaagcaa tacaggagat gcttggacac
     6541 tcggatattt ctacaactca agtgtacagc aagataaata agagcaggat aaaagaagta
     6601 tacagcaaga ctcatccaag ggcttaaaaa gcccttttta atatttagac agtaaaagac
     6661 gccggacaca ggattctggt tatccaaatt aggaggtaaa aatatggtta gcagagttat
     6721 tctaatagtg ttggacagtg tcgggatagg ggagctgcct gacgcagata tgtacgggga
     6781 ttccgggagc aataccctgg gatgcctggt gaggaatgtg aaagatttca aattgaagaa
     6841 tcttgaagct cttggactag gaaacattga caggagcata ggcctgcaaa ttgcagagaa
     6901 tccgacagga agcttcggca gggcaaaaga ggtctcccca ggaaaggata cgactacagg
     6961 ccattgggaa atagcaggag ttactttgtc ccggcccttt cccacttttc ccgaaggatt
     7021 ccccaaggag attgtggagg cctttgaaag ggctataggt gtaaaaacgc tcggaaatga
     7081 agcagcttcg ggaacggaga taattgcaag gcttggcgat aagcatgtgg aaacaggcta
     7141 tccgataata tatacttctg cagacagtgt ttttcagata gcagcccatg aggaggtaat
     7201 accctttcag aagctgtatg aaatatgtga aaaagcaaga gccttgctga aggacgagta
     7261 tgcggtggga cgtgttattg caaggccctt ccttggaaaa agcggaagct ataaacgcac
     7321 ctccaacaga agagattttt ccctgaagcc aattaaaaag acaatgcttg atattatagt
     7381 tgaaaatggt atgaatgtag cagctgtcgg aaaaattgag gatatttttg cgggggaggg
     7441 tatcagtgag gccgttcaca caaaggacaa tacggatgtt gtggataaga cgctggaata
     7501 catgtcagag gataaaacgg ggcttatatt ttcgaacctt gttgattttg acatgcttta
     7561 cgggcacaga aatgatgcgg aaggctatgc cggggcactt atggcctttg atgaaaggct
     7621 tccagagatt tttgcggcaa tgaaggattc cgacgtattg attattactg cagatcacgg
     7681 ctgtgacccg accacggaca gcacggacca ttcaagagaa tacataccga tactggtata
     7741 cggcaggcat gtaaggcaag ggacagatat aggcacaagg gaaggttttt ccgatatagg
     7801 tgcaacggtg ctggatttgt tggggctgcc catagaaata gacggtaaat cctttaaaac
     7861 tgaaatttat aaaatttaac aaacggggtg attgtgtatg aaggagaact tattgtctct
     7921 tataaatgaa gccggggatt atttaactga aagagcaggg gctgcccccg aggctgccct
     7981 gatactcggc tccggcctgg gtgacctggc ggaggaagct gagaataaaa ggatagtggg
     8041 atataaggaa ataccgggtt ttccggtatc cacagtgcag ggccataaag gaaggcttgt
     8101 attcggcact atgggaggca agagggtagt tttcatgcag gggagatttc actattacga
     8161 aggctacagt atgaaggaag ttgttttccc aatatgggtt ttcaattccc tgggagttaa
     8221 gaagcttata gtgacaaatg cagccggcgg cataaataca agcttcaaac ccggagacct
     8281 gatgataatt aaagatcaca taaattatac caataataat ccactgatcg gagcaaatat
     8341 tgaagccttc gggccccggt tccccgatat gtcggcggca tattcaaagg agctggcgga
     8401 tattatcagg gaatgcgcag cttcagaggg tattaagctg cgggaaggaa catacttata
     8461 ccttacaggg cccagttacg agacacctgc tgaaataaga gccttcagaa tactgggggc
     8521 tgatgcggtt ggaatgtcta ccgttcctga agtattggcg gcaaatcact gcggaattaa
     8581 aactgcggga atatcctgca ttacaaatat ggcagcgggt atactggata ccccgttaaa
     8641 ccacaaggag gtcatggaaa cggccgacag ggttaaggaa agcttttcaa agcttatcag
     8701 gggtgctatg gaaagaatat gagaataaaa tttgggggta atgaaaatgg aattaaggga
     8761 aagaatagaa aaatcaacgg attttataag ggagaataca gggtttgagc ctgagacagg
     8821 catagtactc ggtacaggcc tgggttcgtt ggcagactac atagacaagg aagcggtaat
     8881 tgactataag ggtattccgg aatttcccgt atctacggtg cttgggcacg aaggaaggct
     8941 tatactgggc cgccttgaag gtaaaaaggt aattgccatg caggggagat tccattacta
     9001 cgagggctat accatgcagg aggttacgat accggtaagg gtaatgaagc agctgggcat
     9061 aaagctcctg gttgccagca atgcctgcgg aggattgaat ccaaactttg cagccggaga
     9121 tattatgata attacggatc atataaattt catgggaaca aaccctctga tagggcccaa
     9181 ccttgaagaa ttcgggcccc ggtttcccga tatgtcacag gtgtacgaca aggaattggt
     9241 tgaactgctg gagaagattg ccgcgtccca gggcatccga gtgcacaagg gtgtttatgg
     9301 agcggtaagc ggaccaaact attgtacaaa ggcggagctt tcgatgatga taaagctggg
     9361 agccgacacg gtgggtatgt ccacagtgcc ggagacaatt gccgcaagac actgcgggct
     9421 caaggttgcg ggagtgtcct gcattacgga tatggctatt cccgacacaa tgacagcgcc
     9481 ttcccatgag gaaatagtaa aggttgccga gtcggttaaa ccaaggtttg tttccctggt
     9541 taaacaattt atcaaagagg tgcggctgta atgaggatgt atgatatcat aaccaaaaaa
     9601 agaaacggag aagagctgac aagggaagaa atccaattct tcataaatgg gtacaccgac
     9661 ggctcaatcc ctgattatca ggcagcggca cttcttatgg ctgtttactt taagggaatg
     9721 agcaaaagag agacagcgga gcttactatg gctatggtaa attcgggaga gacggttgac
     9781 ctttcgaaaa ttgcaggtgt gaaggttgat aaacattcca cgggaggggt aggagataaa
     9841 atcagtttga ttgtcatccc tctggctgcc tccctgggta ttcccgttgc caaaatgagc
     9901 ggcagagggt tggggcatac cggcggaacc atagataagc tggagtccat tccgggtttt
     9961 aggacggaac tgacaagaga agaattcatt gggaacgtaa acaagtataa aatggctatt
    10021 gcgggccagt cgggaaacct tgtgcccgcc gataagaagc tgtatgcctt aagggatgta
    10081 acggcaacag tagacagtat tcctttgatt gcaagctcaa ttatgagtaa aaagattgct
    10141 tccggtgccg aatgtattgt tcttgatgta aaagcaggtt cgggcgcttt tatgaaatct
    10201 gttgaagagg cagcagagct tgctgggaca atggttgaaa taggcaagtc cctgaacagg
    10261 aaaacagtag cggtagtaac ggatatgagt cagcccctgg ggcatgaagt gggtaattcc
    10321 aatgaaataa ttgaagccat tgacgtattg aagggaaagg gttcggagga cgaaagacaa
    10381 gtggctttga caattgcttc ctatatggcg gtacttggag gcgcttttga agaatttgaa
    10441 aaagcacatt ccgcattgga agaagtaatc aggagcggca aggctattga taatttaaag
    10501 ctgctgatac agattcaagg cggagacccg gatgtggttg acaatactga aaagttgccg
    10561 aaggccaaat atcatataga agtaaggtca aaaaagagcg gctatgtaga ttcaattgat
    10621 gcggaaagca taggcatttc ggcaatgctt ctgggtgcgg gcagaaaaac caaggaagac
    10681 aaaatagact tttctgccgg aataagcatg aaaaagaagg ttggggataa agtagccgtc
    10741 ggcgacattc tgtgtgtgct tcatactaat ttgggcaata ctgctgaagc tgagaaaatt
    10801 gcactggaag catacggcat tggcagtacc gaaaaaaaga ttgtcaaata tatatatgag
    10861 gtaatacagt aattaaggaa tagaaagctg gtgggctgcc tttctgtcat tctttaagct
    10921 taatgcaata tatttgcatt gcataacgaa atcttctaaa gggcaatata aaagcaaaca
    10981 aatcaagtag gaggattgtt atgtataatt cggcaaatat aaataaatta tcccttaagc
    11041 tgatatgtta tattaccata attgcaattg cccattttgc attaaatatc cctgtatgcg
    11101 cagaaaatga tatgaacctt aactgtaaat cagcggtact tatggatttc ggcacgggca
    11161 aaataatata tgaacagaat tcccgcgaaa aattaccccc tgccagcgtt acaaaggtaa
    11221 tgacaatgct ccttattatg gaggcattgg acagcggaaa gataagctat gaggataagg
    11281 ttacaataag cccccaggct tccagcaaaa acagcaaggg tacaatgctg ctgctggagg
    11341 ccggtgaagt caggacggta aaagaactct tgtacggcat agccgtggag tcggcaaatg
    11401 atgcctgcat tgcaatggct gagcatatat ccggaagtga agaggaattt gtgaagctta
    11461 tgaacaggag agcaaaagag ctgggagcaa atgacagcaa ttttgtaaac cccaacggcc
    11521 ttcctgagga cgggcatgtg acaacggcct atgatctggc tgtattgtcc agagagcttt
    11581 taaagcatga aaagatattt gatttcatat cacagtatat ggtcaaggtc tatgtaggaa
    11641 agaaaaacaa cgtattgaga gaattggtaa ataaaaacaa gatggtcaga ttctacaacg
    11701 atgtggacgg tataaaaacg ggatggactg aggaagcggg gtattgcatt tccgtaactg
    11761 caaaaaggaa taatttaagg cttataagtg tggtaatggg ttctccgaag gtggatataa
    11821 gaaataaaga agcccgaaag cttattgatt acggatttgc aaatttcaac agcctcataa
    11881 tagcgaaaaa gggtgataag atacaggaag tgaatgtatc aaaaggcagc aggctgaagg
    11941 taaatgcatt ggcggaaagc gatgtttcgg tgctgctgaa caagggtgag gagaaaagta
    12001 tagtaaaaaa agcagagctg cctgaggctc tcaaagcacc cgttaaaaaa ggcgaaaaga
    12061 tcggagagct ggtaataagc attaatgata aagagattgg gaggcacaat ttgatcagcg
    12121 acgctgacgt aaacaaatct acctttctca ataacttaag aaatgcaata atatattggt
    12181 tcggaaattc gaactgacat acacttcccg gctatgccgg gttttttctt tatattgtat
    12241 aaatagcgca aatgtattaa aatatattag atatattatt tttccataca aaggagttat
    12301 tccgtgactg atttgttatc aaaattttta tactatctga acttgaactt tggcatatca
    12361 tctttattat acagtctgcc gggcataatt ctcgcattga cgatacatga atactgccat
    12421 gctctggcgg cttacgcaat gggggataat acagccaaac agtatggaag gcttacgtta
    12481 aatcccattg cccatatgga tcctttgggt tttctgtgcc tgatagtcac caggcgattc
    12541 ggctgggcaa agcctgtgcc ggtaaatgat atgaatttcc gtaaccgaag aaagggaatg
    12601 ctgcttgtat cccttgcggg cccgatgtca aatttcataa ctgccatgct tattgccgcg
    12661 gcttatgctg ttttttacaa aagcatggga gagataacca aaggtatttt gcagattgcg
    12721 tatataataa acctaagcat aggcgccttt aatctgcttc cgataccgcc tctggacggc
    12781 tcaaaaatac ttgatgcttt tctgaatgac agacagaggt tttttatgag aagatatgcc
    12841 atgtattcaa atatgcttat gcttatacta attttttcgg gaatcatagg cagagtattg
    12901 gggcctgtca taatggttat ggattggttc attaattctg tcataggatt gtttatttaa
    12961 aagatagcac tggaggtaat taaaatgaca agacagagaa ttatgagcgg tatgaggcct
    13021 acgggcactc ttcacctggg caattatttc ggagcactgg agaattgggt aaagctgcag
    13081 aatgattatg aatgcttttt ttccattgtt gattggcatg gcttaacaac aggctatgaa
    13141 gataccagtg aattcaggaa caacacaatg gaaattgcaa tagattggct gagcgcaggc
    13201 ttggatccgg aaaaatgtac catattcgta cagtccaatg tcaaggaaat tgctgaactg
    13261 catcttttgc tgtcaatgat tgtaccgctt tcatggcttg aaagatgccc tacctataaa
    13321 gaacagcttt cacagcttac cgagaagaac ataacaactt atggttttct cggatatcct
    13381 aatctgatgg ctgctgacat acttgttcac aaaggaaatt ttgtgccggt cggtgaggac
    13441 cagcagccgc atttggaact tgccagggaa atcgcaagaa ggtttaatta tatgtacggg
    13501 gataccttcc ctgaacctca ggggaaaatg acgaaaataa aagtgcttcc cggccttgac
    13561 ggaagaaaaa tgagcaaaag ctatgacaat acaattgcaa tgtcggattc tcccgagact
    13621 atcagggaaa agataaggca aatgattacg gatgctgaaa aaataaagaa gaatgacccg
    13681 ggacatccgg acatatgcgc ggtatatgca ttccataagg ttttttccga gaatacacat
    13741 caggaaactt atgatgtatg caagaccgga ggtataggct gcgtacaatg caagaaggtt
    13801 cttgcagaaa atgtagttca gtatatggcc cccatgtatg agagaagaca gaagctgctc
    13861 ggcaataagg aggccattct ggaaatactg agagagggaa cccgtcatgc cagagaagag
    13921 gcaagggaga caattaagaa agtaagagaa aagatgaaac taatggaatt ggagtaacac
    13981 atggctataa atgttaagct ggaagccttt gaaggtcctt tggatcttct gcttcatctc
    14041 gttgaaaaag cagaggtaga catctatgat ataccaattg ctgagataac ggatcagtac
    14101 ctccattata taaatatgat ggagatacat gatcttgagg tttcaagcga atttattgtt
    14161 atggccgcaa ccttgctgga gataaaatca aagatgctgc tgccgaagcc gaagaaggaa
    14221 gaaaatataa ctgatgagat cgatccaagg gaagagcttg ttctaaagct tattgaatac
    14281 aaaaagtata aggaggcctc tcttgattta aaggacaagc tgggcatata ttccaaggtt
    14341 ttttacaagc ctcccgagcc catagaggat tacatagatg atttcgcctc tatttccaat
    14401 gtttccttgg acatgctgta tatgagcttc agaaatataa ttttgaagaa tgaatcaaga
    14461 aggaaaaaca gctacacgga aatctacagg gaggttgtca ccgtagaaga caagataagg
    14521 ctgataaaca aattgcttgc cggcaaaccg tcattttact ttgacgactt gtttatcgga
    14581 tgttacaaca ggtacgagat aattgtcact ttcctggcga tacttgagct gctgaaaaga
    14641 agaagcctgc ttattgaaca ggaaaagaac ttctcaagga tacttattaa gagaaggaca
    14701 aaaggtgcat gaagggttgg gttttgttat ggatataaga gaaataaagt cgattgtaga
    14761 ggctcttttg tttgtagccg gagatgctgt tcccataaag gagatatgca gtatcatcag
    14821 tattgacgaa actacactca gaagaatagt aatgcagatg atggacagct ttaatgaaga
    14881 taacagagga atccagatta ttgaggtaaa caactgctac cagatttgca caaagccgga
    14941 gcattatgag tatatagaaa ggctggtcaa gccccaaagc cgccagggcc tttctcaggc
    15001 atcccttgag acacttgcga tagttgcata taaacaaccg ataaccaagt ctcagattga
    15061 ttatatcaga ggggtaaaat ccgacggatg catcgcaaga ttagcggaaa aagaattgat
    15121 atatgaggca ggaaggctgg agggcccggg cagggcgata ctgtacggga ccacagacaa
    15181 tttccttaag ctgttcgggc tgagatccat taaggagctg cctgctctga tagaacttaa
    15241 tggagaagaa ggagcttaga gggctgtgtg catttcaaag ccttgttaac cttctgcact
    15301 ttgtctttat cctatacatc agatgatgta ttttttttta ttttttgaga aagatataca
    15361 gattggaaaa gagtagagac acggggacgt ttctcctgtc tcactgaaat gagacacgcg
    15421 aaacgtcccc gtgtctcatg cttggaggaa ttgtgatgct gtatgctgct tccgggttca
    15481 tatttttaat attgatattg tatttgatgc ccgtagaaat aagcacgaag gttgaaaagc
    15541 acaatagcaa tgtgaagatt tccttggtgc tgaagacctt atatggcttg ttcaatgtca
    15601 ataccgaaat tcctgcattt aagctggatt atgaaaacgg aaagcctgta ttgaagtata
    15661 aggttgaagt agcaaataag gaaagaagca ggttattggc cggatttagc aagcttctta
    15721 ctattgaaga aggagaaggc ttatacagga tatataaaaa gaaaaaggat ataattttac
    15781 cacctttaaa atatttaaca aaaaaaattt ctgttagaaa tctttacctg aaactaaaaa
    15841 tgggaaccgg tgatgccgca gcaacgggat tgttgtatgg aacagcatgg atagcggtag
    15901 gcaatataat ggcacttacc cgaagctatg ttaacattaa ggagcccagg ataataattg
    15961 ctccaatatt cgacagtgcc aggctgagcc ttgatttcag ttgtataatt cgcattaaaa
    16021 caggtcatat tataaatgcc ggaattaggg taatacctgc attgataact aaattataga
    16081 aggatgtgtt tagcttggat cagcctgttc aaagcttgat gaaaactaca atggaaaaca
    16141 tcaagaacat gatagatgtt aatacggtag ttggtgatgc tgttgaaaca aaagacggaa
    16201 ccgttattgt accagtgtca aaggtatcct ttgcattcat agccgggggc ggagaatata
    16261 cgggagcaac cgataagaac gaagaggatg cttcaaagta tccttttgca ggaggaagcg
    16321 gagcgggggt aacggtacag cccataggtt ttctggctac aacaaacgga cagctgaggg
    16381 tcatacccgt caggtacagc agtgccgttg acagaatagt agatatggtg cctgacctta
    16441 taaataatat ggagaattat ttaaaaaaga aaaggcattc tgaagaagac agccttgaaa
    16501 tgtaagaagg agtttaactc cttccttttt tacataaaaa tgctataata gacttgataa
    16561 tatagaaagt tttgattatt atccttaagg aatgatatgc tttatcggga ggtaattata
    16621 tggagttaaa aagtgcagtt gatatgatat gggaaaacag aaaatacttg actgatgatc
    16681 ctaaagaagt attgagccat ttgaatgagg aagttgccga atcattgaaa gccctgctca
    16741 gaggtgagga agacagggct aagagtgaat tggaagatgc tctctcatgt attcttattg
    16801 cgctgaaggt aatggatgtt gatattgaag aagcagtaca aaggcaaatt atacaaatga
    16861 agaaaagaca tgacaagctg atgattttta agaaagacaa ggtagagata tatgtagacg
    16921 gaacattaaa aggaggctgg actatctggg gagaggatga cataaaggaa gcgctcaaga
    16981 ttgccaagga atttggctgc aaaattgaca aggaataatg gaattatata aatatatcat
    17041 tctgttaata atatatgtat gcatttaata ttgagatgta aatcatttat tatttcggag
    17101 tgatttgatg aaaagaaata cggctatagt catggcagca gttattgctg cattatcagg
    17161 cttttttgca gtgcaggctt tgcaggaatc cagtgcccgc gctgcagtag tcatggatgt
    17221 aaactccaac aggatactat attccaaaaa catggacgaa aagatggcca tggccagtac
    17281 tacaaagata atgacaactc ttgtagctat tgaaagcgga agattggatg aaaaggttac
    17341 aataagcaaa agagcttctt atatggaagg ctcctccata tacctgcggg aaggtgaagt
    17401 gcatacagtc aatgatttac tgtacgcaat aatgctccgg tcgggaaatg atgccgcaac
    17461 ggctgtagca gagcatatcg gcgggtcggc agagggtttt gcggaaatga tgaataaaaa
    17521 ggcccgggag attggagcaa ataatacaag gtttgccaat ccccacggcc tggatgcgga
    17581 ggggcattat accactgccc gggatcttgc tctcattact tcttatgctt tgaaaaaccc
    17641 tctgttttcg acaatagtaa gcagcaaaaa aaaggtcatg gaaggccctc caagtgaaaa
    17701 ctgggataga gtaatgatta ataaaaacaa aatgctgtgg cagtttgacg gaggagacgg
    17761 agtaaaaaca ggttttacaa aaaaagcagg gcgatgcctg gtatcttccg ctacaagaga
    17821 aggaatgcgg ctggtttgtg tagttttaaa ctgcgggccg atgtgggatg aatcatcggc
    17881 tcttctggaa tatgcattta aaaattatgt aaaaaagaga attgtagata aggacagcat
    17941 ttttaaggtt gtggaagtaa ggaacgggaa agaaaggttt gtcggagtca agcctgcgga
    18001 ggattttgac ctggtgttga gaacagacag ggttgaaaat attaaattgg aagctaaaga
    18061 tttgaaagcg gcgcaggccc cattaaaaaa aggcggccat gcgggaaggc ttgaaatata
    18121 cattgacaat aatttgttga agacaataaa tcttgagtac tcggaaggtg tggagtccag
    18181 cagccctttt tattatctga aacgaattct gacagattat atagcagacg caaaacagta
    18241 agaaatatct tttatgggaa aataggcttg atgcttatca agaaggttta tataatggca
    18301 gattacctta aattaagcat atctcaaagg ttttaccttt tggctgatat ttaatgatta
    18361 attgtgaggg gtagggggaa atggcggaga gactgcaaaa agtaatggcg gagtacggag
    18421 tggcttcaag gcgtaaaagt gaagaaatga tatgtgccgg aaaggtgaag gtcaacggca
    18481 ggttggttac ggaaccgggc ttcgggattg ataaagacag agatgtgata gaagttgacg
    18541 gggatatcat caaaagcccg gagaaaaaaa tttatatttt actgaacaaa cctactggat
    18601 atataaccag cgtaaaggat caattcggaa ggcccactgt cttagacctg ttaaagggaa
    18661 taagcaccag ggtctttcct attggacgcc tggattatga tactgagggc cttttgattt
    18721 tgagcaatga cggtgaactt acatatagaa ttacccaccc taagcacagt atcgataaga
    18781 cttacagagc tttggtccga ggagaagcgg acaagagtga tattaaaatc tttgaaaggg
    18841 gtataactat cgaggattat gttactgctc ccgcaaagat ggaaattgta agatactcaa
    18901 aaggcaacag tgtaattgat attaccatac atgaaggcag aaacaggcaa gtcaggaaaa
    18961 tgtgttccgc cataggtcat gaggtaataa ggctgcggcg gataaaaata ggagatatcg
    19021 ggctgggagg gttaaaaacc ggcgaatgga gatacctgag ggattcagaa ataagatatt
    19081 taaaaaaact ggggggcata tggaatgatg cagataagaa aaataaaacc tgaggacata
    19141 agctttgtaa ttgagcttgc ttcaaaatac ggattttctg acttaaagct tttgtctaat
    19201 atagagcatt tccttatatg cgaggatggg aataccaaat gcggatgcgg ctgtctggtt
    19261 ccgtataagg gaaaaggata tctgagctgt gttatagtgg atgaaggcca tagaagaaaa
    19321 aagcttgggg gtgcgataac aaaagcattg cttaatatag ccgatctgca gggaattgag
    19381 gaggtatacg cagcaggaat ctgcaaggac tttttgcagg caatgggctt cgagaagtat
    19441 aatggcactg atttagaggt tgacttatta gaagcaattg gcgatatagg catatcagaa
    19501 tactataggg tgcttctaaa agattatttt aagccttgtt cgcatatata aaaagtaact
    19561 tttttgtaaa gaaacatttt tttttattta ataatgtgct atattaataa taattatttt
    19621 tattacagca tattcaataa ttgcagaaaa tatacaggag gaaataaata tggcaaggat
    19681 taaaattaca gaaacatcat tgagggatgg acaccaatca cttattgcta caaggatgag
    19741 gacggatgaa atgctgccga tcatcgagct tatggataag gtgggatatc attcattgga
    19801 aatgtgggga ggagctacct ttgacacatg cctcagattc ctggacgaag acccatggga
    19861 aagacttcga ttaatcaagt caaagcttaa gaatacaaag ctgcaaatgc ttctcagagg
    19921 acagaatata c
//
LOCUS       DAOOAB010000049        19663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3671_length_19663_cov_11.878876, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000049 DAOOAB010000000
VERSION     DAOOAB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19663
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_19663_cov_11.878876"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..571)
                     /locus_tag="PKU88_09895"
     CDS             complement(5..571)
                     /locus_tag="PKU88_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69625.1"
                     /translation="MKLIFSIESDWEGSAKKQSSILPMLQCINGVYPSVKYIFRTANT
                     KDELRYCLRKFKQVSRTDNDYCALFFSGHGSTGKVFIGEEPLTLLELAEAANEVGEKL
                     FKGHLVHFDSCSVVKDSEQIVKEFIKMTGAKLVSGFCNDVDFIESYALEMIYIDYLSD
                     SNTPYKAYKEVIKNHSELSKRTGFIAIR"
     gene            complement(808..1338)
                     /locus_tag="PKU88_09900"
     CDS             complement(808..1338)
                     /locus_tag="PKU88_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69626.1"
                     /translation="MRDIVLLAKLIKTKNEIEREISAITDRPAIIGHVGEYITSKIFG
                     IQLEQSASNKGFDGYFIEGNLAGKSVNIKWYTKQNGILDINPETLPDYYLVLAGPHTA
                     SNSSKGTTLPWLIEEVFLFNSKELMEKLINRNAKIGVATSVAMEYWMEAKIYPISNNN
                     HLTLTDKQIEELSLFK"
     gene            complement(1584..2099)
                     /locus_tag="PKU88_09905"
     CDS             complement(1584..2099)
                     /locus_tag="PKU88_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69627.1"
                     /translation="MNEGADFPFVECLFFLRPTESQRVFYQQLGRGLRKYVGKSHCII
                     IDFIGNFKNAYKLVEYQGLQPFKEESSDSFTWSKNPKDILNLPLGCTVSFDDKVLDIF
                     AQQTLDPRTATRHNIGRILIYQYRKLCRRLERIATKADVDRNLILNSDFYKLVFGSWK
                     KFEEIIDGYEF"
     gene            complement(2096..3499)
                     /locus_tag="PKU88_09910"
     CDS             complement(2096..3499)
                     /locus_tag="PKU88_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPX69628.1"
                     /translation="MGSLIKVEEFECFKAVREVSPSLINDQILSAVRNLDEKEELEPF
                     IRSIIYDYNETPHGPAEIADIFTHRVTVNREQGMAAFILKEKSFKTIRPTHVAHQIYR
                     LEKISGLKFAIFAATGIILDSVKEHFCTTAERQNCKYAIFDATDLGRLFIAYGFLCPR
                     DARRIIAGRCKCGYSPHNRIHNVFQNEALKALEEARAIGQCAGLIILPPSSGKTRIAA
                     EDSKKAQSKKVLYVAHTHEILDVAVSEFEAIYRKTDVRLHETEASLSELATINIATIQ
                     LLQRHINRISSEQFDYLIIDEFHHAAAKTYRKLIEKVKHSFLLGLTATPYRGDRQDIL
                     ELCNNNIIVNYEMRTGIDMGILSPYHYFGCFDNVDYSNIKMKGNNYDICDLEKALIIP
                     ERDEAIIQKWKEKANEKPTIAFCCSHRHAERMAESFIKHGINAEVYLSDTAKDKRAAL
                     ISDLQQRDIKLFVQLMC"
     gene            complement(3704..4156)
                     /locus_tag="PKU88_09915"
     CDS             complement(3704..4156)
                     /locus_tag="PKU88_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69629.1"
                     /translation="MQYRVHPQDKPEIKFRLNEQKYELIRDISVGQKCTAMLIMALSD
                     GNFTIVIDQPEDSLDVRSVWDDMCTKIRKGKEKRQFILTTHNSCLAVASDTDKYTIVE
                     SDAISGSIVISGALESDPVKEEVIKYLEGGRPTYHKKASKYGMKGYFA"
     gene            4353..5897
                     /gene="istA"
                     /locus_tag="PKU88_09920"
     CDS             4353..5897
                     /gene="istA"
                     /locus_tag="PKU88_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007951521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HPX69630.1"
                     /translation="MTKYREILRLYSQGISQRSIALSCECSRNTVAKVVARAKELNIT
                     WPLSSEITDGELEKKLFPNSQQSTTVPRKYPDLEYIHKELAKNGVTLRLLWSEYCEEC
                     RLSNELPLMYSQFCYHYQQFAEKKRATMHIPRKPGEQIEVDWAGQTAAIVDRDTGEII
                     SAYVFVGVLSYSQFAYVEAFLSQDQESWISAHVNMYRFFGGVSRILIPDNLKTGVEKV
                     SWYTPVINKIYHEMAEHYDTAVIPARVRKPKDKPNAEGTVGIISTWIIAALRKQKFFS
                     LQELNVAIKEKLHSFNSKPFQKREGSRTSIFLEEEKSLLLPLPTNPYELAVWKQATVQ
                     FNYHITVDKMHYSVPYEYIKYKVDVRVTRNVIEVFYHNHRISSHPRLYGRASQYSTND
                     AHMPEDHQNYLQWNAERFLSWAEKIGPNTNITVKAILSSHKVEQQGYRSCIALLKLAD
                     KYSVPRLETACKKALSYTPYPSFKSVKTILSTGQDKVTEEQTAKTNDDSANYGFTRGA
                     DYYGRK"
     gene            5901..6656
                     /gene="istB"
                     /locus_tag="PKU88_09925"
     CDS             5901..6656
                     /gene="istB"
                     /locus_tag="PKU88_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HPX69631.1"
                     /translation="MTNEATINKLLEMRLTTMADAYRRQLQEHSLDNFSFEERLGLLV
                     DIEYTSRKNNRLKRLIHSAGFDQSYACIADINYSAGRKLDKSQIERLASCQYIAEKHN
                     IIIMGASGAGKSYLSCAFGIEACKQYYTVKYIRLPELLADLAVARGEGIYKRVINQYR
                     KVNLLILDEWMLVSLTETESRDLLEIIHARHKRASTVFCSQFSPAGWHGKIGETTLAD
                     AILDRIVHDSYTIEIHGSESDRSMREVYGIQNK"
     gene            complement(6695..8956)
                     /locus_tag="PKU88_09930"
     CDS             complement(6695..8956)
                     /locus_tag="PKU88_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPX69632.1"
                     /translation="MNKHSGLSFQKFDLHVHTPGSHDFVDKSVTPKQIVEKTIEKGLK
                     GIAITDHNTGDWIDKVKKEANNTNLVVFPGVEIYCTGGKDGIHVIGILGPDKGTKHIE
                     AILAKLKINPDEYGTKNAVSTLSPYQVIDVISECNGIGVLAHCTSSKGVLHDITGLTR
                     TKIFENPALLAVEASYHDFIDPEKIKKHIRAIDLLDGKDENYNFRRLGVYIASDSKGE
                     NDDNHNLNAIGSKYTYFKVDDEIDLESLRQCFIDRDVRIRQYFEYKEDFYPYIKAIEV
                     KGGFFDGQVATFHSGLNSILGAKGSGKSLLVELMRFVLGQPSSQKEILDDHKRKLEKK
                     LETYGSVKIKFIDETGVENEIERTYNPSKNNPYNKEEQERIASSFKILFLSQNEIVKI
                     AEDENEQIRFIDRFFDFQHYQNRVKNTEIDLSELDIQFAEGLRSVHILNDVRKQISKN
                     NSELEKLNKLLSDPIYDNYKLLEQKDRALKVQESYLNTFREYIISQKLEAENLDIPSF
                     KEPLVKDPAIMRNDDIIKKSKSYVIDLLNKAIEDIEKAIRSVQTEYKKWNIMYLGEKQ
                     KYEEHIRNAGGDRKALENQRLKVLKESEDLKKREEILVKKTSNLKNINKQRDNIIKQL
                     FEIYNEYSNERKSKCEKFEKESNGRLQIKIYESTNIDEFKNKLCALKRGSYLRDTEID
                     ELCSKVTPYDFILTVLRYEVSHELDRLKPLSKKIGIEVQRLQALCEFLLSCVYRCGGA
                     TIPAGGEPPVRNK"
     gene            complement(9279..9497)
                     /locus_tag="PKU88_09935"
     CDS             complement(9279..9497)
                     /locus_tag="PKU88_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HPX69633.1"
                     /translation="MPSWKELKRFCENDGWELYKTTDHYFYRKIDASGNIRRTKVSMG
                     SGEIEKNFFRIILKKQLGVTKEYFNNKL"
     gene            complement(9476..9901)
                     /locus_tag="PKU88_09940"
     CDS             complement(9476..9901)
                     /locus_tag="PKU88_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69634.1"
                     /translation="MEAINATDVRKDWGRFIDSVVRNKPKFIKRSRDYIFAASTDMLN
                     EMLKEYTLTATVYEEENGTITAELEEIDIAANGSTEEEALDILASDLQEYAEEYYNEF
                     EYWYNAPNRKAHLPYVLKVLLQEETKDILSMIKCQAGKS"
     gene            complement(10136..10711)
                     /locus_tag="PKU88_09945"
     CDS             complement(10136..10711)
                     /locus_tag="PKU88_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HPX69635.1"
                     /translation="MWIYEKKLQFPAWVEGCDLHIAKLLFAQYGGPDSELSAGLRYLS
                     QRYTMPIPEAKAVLTDIGTEELAHWEIIATLIFKIVDRATPEELIEAGMGPNYIQHGR
                     DVYPNDAAGVPWTAAYIQAIEDPITNLHEDLAAEQKARTTYEHLINSTKDRGVIETLS
                     FLREREVVHFQRFGETLRLVQEHMDNIKHKR"
     gene            complement(10715..10987)
                     /locus_tag="PKU88_09950"
     CDS             complement(10715..10987)
                     /locus_tag="PKU88_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HPX69636.1"
                     /translation="MDISRDRLELLKEIMAIDFSLVELNLYLDTHPMDQRALRLHNEY
                     VMKSRMLKEKYEKEYALITHKFISDCPWEWIEDPWPWEIDYEMGGR"
     gene            complement(11007..11189)
                     /locus_tag="PKU88_09955"
     CDS             complement(11007..11189)
                     /locus_tag="PKU88_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HPX69637.1"
                     /translation="MLVAPHYEIPVMGPEFKLAVAYIPYQVLQKVYEPMKGLMRGTIF
                     PELYRPYVKMKKDRDD"
     gene            complement(11380..12468)
                     /locus_tag="PKU88_09960"
     CDS             complement(11380..12468)
                     /locus_tag="PKU88_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69638.1"
                     /translation="MQDREEFKKVLLMQHERYPGMQIADMVKLIYQNEFAGGHLITNE
                     DECLKRLTEELRILEKDSGAYIPPVPFENIGNGLCRFYLVSEQISGIDIGTLNRFFIS
                     TSNSIRGNVQSFEQKLKVFKECCADGDFPYKPEEAEAWLDEYRRRGYPPMHHSGEYKA
                     LYSPAYRIVKLDYIHYLEIFRKIDSLLKSRDRVLAAIDGNCGAGKSTLAALIGDVYDC
                     NIFHMDHFYLRPGQGTRERMEEAGGNVDYERFREEVIDGILRGSEFKYRIYDCKKGVM
                     DEYITVLPKKLNIIEGAYSMHPALIDYYDLKIFLGIDEKTQRDRILERNGEVILEQFL
                     TKWIPMENRYFEQLKIEEKCDLVYRNNR"
     gene            complement(12458..12994)
                     /locus_tag="PKU88_09965"
     CDS             complement(12458..12994)
                     /locus_tag="PKU88_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPX69639.1"
                     /translation="MERLSIKNLVLSGFFIAFGLVLPFLTSQIPSLGSKFLPMHLPVL
                     IAGFVCGWQYGLIVGFIVPVFRSMLFGMPPMFPTAAAMAAELAVYGCMTGLMYKLLPK
                     RDAYIYAALLISMICGRIAWGLAGVFLYGLGGTSFTWEVFMAGAFINAVPGIIIQLIL
                     IPIIIIALKKAKVMQNAG"
     gene            complement(13179..13790)
                     /locus_tag="PKU88_09970"
     CDS             complement(13179..13790)
                     /locus_tag="PKU88_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HPX69640.1"
                     /translation="MIDSGISIDARTANSFYDRERAKAYIDAYWKNYNPAYPSFSHGG
                     GDCTNFVSQVLYAGGMPWADDKNPANHKKSINWYCKPGATAKDSENRITFSWKIAAVF
                     KAHWISRAARHRILSYTEAMQSINWLSDSVFPGDVVQFCYSGGAPYHTLAVTGYNRDP
                     EYNIRDIVLASHDMDSNTRSLYRTMLKYPSDYKLRVYNIKLGN"
     gene            complement(13882..14056)
                     /gene="ssrS"
                     /locus_tag="PKU88_09975"
     ncRNA           complement(13882..14056)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKU88_09975"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(14145..14315)
                     /locus_tag="PKU88_09980"
     CDS             complement(14145..14315)
                     /locus_tag="PKU88_09980"
                     /inference="COORDINATES: protein motif:HMM:NF020945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HPX69641.1"
                     /translation="MEEINEVLMKIEELRKKMNDMIYESDDLLDSKIIELSRLLDEQL
                     TEYYRLLKKKNE"
     gene            complement(14537..15763)
                     /locus_tag="PKU88_09985"
     CDS             complement(14537..15763)
                     /locus_tag="PKU88_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPX69642.1"
                     /translation="MSEIINNREYRQKVLKELIMELHAGKTVEEVKSRFEELIKGVSA
                     SEISEMEQALIMEGMPVEEIQRLCDVHAAVFKGSIEDIHKVRKPDETPGHPVHTFKLE
                     NRELEKLINERLKPHLAGFRASDNSENIYKLLEDINLLLDIDKHYSRKENLLFPYMEK
                     YGITAPPKVMWGVDDEIRAAIKEIKGMLQDYKGEKEQVYTKADEAAGRVIEMIFKEEN
                     ILFPMVLETLTEDEWMTIESESDEIGYCLTEPRGKWKPERTDVEEKQKETGQTAADGY
                     LKFETGLLTAKEISLMFNSLPIDITFVDKDGAVKYFSQGKERIFPRTKAIIGRQVHNC
                     HPPASVHIVEKLVEDFKTGKKDSESFWIKMGGRYVYIRYFAIRDDNGDYAGTMEVTQD
                     IQPIQEISGEKRLVSD"
     gene            complement(15776..16003)
                     /locus_tag="PKU88_09990"
     CDS             complement(15776..16003)
                     /locus_tag="PKU88_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPX69643.1"
                     /translation="MSGEIDLSKTVYELSREYPEIVDIMKELGFESITNEGMLNTAGR
                     FMTIPKGAAMKGISMEKISQAFESKGYSIKG"
     gene            complement(16076..16765)
                     /locus_tag="PKU88_09995"
     CDS             complement(16076..16765)
                     /locus_tag="PKU88_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPX69644.1"
                     /translation="MKIVYTGKTKNVYALEDGNYLLKFKDDVTGTDGVFDPGSNTVGL
                     TIEGAGRAGLRLTKYFYEKLNSLGIPTHYIDADIEAGTMTVKKASVFGKGLEFVCRFK
                     AVGSFLRRYGKYVEEGQDLDAFVEVTLKDDEREDPPITEDALEMLGILSKDEYKVLKD
                     LTQKIAGIIKDELAKKGLELYDIKFEFGRTGEDKQITLIDEISGGNMRAYKNGSPVAP
                     LEIERLMLDAD"
     gene            16965..18011
                     /locus_tag="PKU88_10000"
     CDS             16965..18011
                     /locus_tag="PKU88_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPX69645.1"
                     /translation="MKLKVLWTMAEGMGTASDLEMLKNAKPFDIDVVTAGTKGKDAAG
                     FVITGKKFVVPEVKDFKYLNAILDICRIEKVTTVIPQYTDELLLMSENTKQFNDLGIK
                     VLVTEDADKLRIANSKSELYKYFRQKSFVPEYTLASDIDSIKKAVYELGYPSVPVCIK
                     PVQGEGGKGFRIITDEKIDIFKEPVNSPKLSLEAYLESIKISDSIPELLVTEYLPGKE
                     YSVDCVCKNGTTYICIPRQRIETAMGVSSVSLIEKNEELISYSKEIISSLSLSYNINI
                     QFKYSSEGKPKLIEINPRVSGSLVANYGAGINMLEAALRLAYDMPLENIEIKWGTKMI
                     RQWREIFIQQHPKP"
     gene            complement(18001..>18309)
                     /locus_tag="PKU88_10005"
     CDS             complement(18001..>18309)
                     /locus_tag="PKU88_10005"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPX69646.1"
                     /translation="PTYKMLDSIAQFNLKGGVVLENDEVAAFCLYEKVSGDMAQSHVE
                     LTNNSHRGVHTYLVSELAKRMDEEYINKEDDMGLPGLRRFKEKYNPCTMLKKYKAIMV
                     "
     assembly_gap    18310..18409
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18697..19581)
                     /locus_tag="PKU88_10010"
     CDS             complement(18697..19581)
                     /locus_tag="PKU88_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPX69647.1"
                     /translation="MNFRDFLANLQSVRDYEEENLKDLVLSDIRVYLEKINADAGKEK
                     GFSLIMLKNGSDVFNALKGVGGYSGVMIKSPHYIGLAVTKENFEDEFFGAYHMQSVVK
                     KLQEMYLGSCWIDIRDVSGDIKAKLSGDSESRINYLLAFGIPDEKAIKNKKPRTSVTN
                     EASEYRQNPYGIKYADAEITDRARLALGEIVYLHEWGRPAAYEELESRGMADLFFYIR
                     NAPSYKNLQPCRIILKDGEAELTVLEPQNPRNYIDAGIMMYTMEGLAKDLGIPGKWSF
                     VGPKAGNKGYGIAAHIEL"
BASE COUNT         5424 a         4433 c         3283 g         6423 t
ORIGIN      
        1 tctattaacg tattgctata aatcccgttc gcttactaag ttcagaatga ttttttataa
       61 cctctttata tgctttatac ggtgtattgc tatcactgag gtaatcaata taaatcattt
      121 ctaatgcata tgactcaata aagtccacat cattacaaaa tcctgaaact aatttagctc
      181 cggtcatttt gataaattct ttaactatct gttctgagtc tttaacaact gaacatgagt
      241 caaagtgcac aagatgacct ttgaatagtt tttctccaac ttcattagcg gcttctgcca
      301 actcaagtaa agtaagcggc tcttctccaa tgaaaacctt tcctgtagaa ccatgccccg
      361 agaaaaatag agcacaatag tcattgtctg tccgactgac ttgcttaaac tttcttaaac
      421 aatatcttaa ctcgtccttt gtgttagccg ttctgaaaat atattttaca gatggatata
      481 cgccatttat gcactgcagc ataggaagaa tcgaactttg cttttttgca ctaccttccc
      541 aatcagactc tatgctaaaa atcaacttca tcttaatccc ccttagaaat tttgtataac
      601 ggttccgtac tcacgacacc actgaagcgg attctagaac ttgtcccttt ggcgaagtga
      661 ccctcccgat gaagtggtgt ggttgctact aaagttgaat ctcagtctat catatcccct
      721 gcaacctaag gcacgcacga tacttgctct tgctaccgca ctgcctccta agaactttct
      781 tatatgcaga ccactgccta cttaagtcta cttgaataaa gaaagttctt caatttgttt
      841 atcagttagt gttaaatgat tattatttga aataggatat atctttgcct ccatccaata
      901 ctccatagca acacttgttg ctactccaat tttggcattt ctatttatca atttttccat
      961 aagttctttt gaattgaaca agaatacctc ttcaattagc catggcaacg tagttccctt
     1021 tgaagagttt gatgctgtat gtggcccagc taatacaaga taataatcag gaagtgtctc
     1081 agggtttata tcaagtattc cgttttgttt ggtgtaccat ttgatattta cacttttacc
     1141 ggctaaatta ccttcaataa agtaaccatc aaatccctta ttagacgcag attgttcaag
     1201 ttgtatcccg aatattttgg atgttatata ttctccaaca tgtccaataa ttgcaggcct
     1261 atctgttatt gcagaaattt ctcgttctat ctcattcttt gtttttatta atttcgctaa
     1321 taatactata tctctcatta agaattctcc ttctagtgtt caaactatta ttactaagtg
     1381 tatttcacat aacaggttca cactcaagat accagtaacc caggccctag tgcctgtccc
     1441 tttggcagag cttcgctcct ggtgaatggg tgtggttgct gcttaagtca cactatagct
     1501 ttgcaaatcc tctgcaaccc aaggtattca tgattcttgc tcttgcaatc gtgtatttgt
     1561 atgacaaatt gagccaaatc ttactaaaac tcatatccat ctataatctc ctcaaacttt
     1621 ttccaacttc caaatactaa tttataaaaa tcactattta aaattaaatt tctatccaca
     1681 tccgcttttg ttgctattcg ttctaacctt ctgcatagtt ttcgatattg atatataagt
     1741 atcctgccaa tgttatgtct tgtagcagtt cttggatcaa gtgtttgttg tgcaaatata
     1801 tctaaaactt tatcatcaaa actgacggta caacccaaag gaagatttag aatatctttt
     1861 ggattttttg accaagtaaa tgaatcagat gattcttctt taaatggttg aagcccttga
     1921 tattcaacaa gtttgtacgc attcttaaag tttccaataa agtcaattat aatgcaatga
     1981 gattttccaa catattttct aagccctcta cccaattgct gatagaatac cctttgagat
     2041 tctgttggtc ttagaaagaa caggcattct acaaaaggaa agtcagctcc ttcattcaac
     2101 acatcaactg tacaaataat tttatatctc tttgttgtaa atctgatatt aatgctgctc
     2161 gcttatcctt agcagtatca gataaatata cttccgcatt tattccatgt ttaataaaac
     2221 tttctgccat tcgttcagca tgacggtgag agcagcaaaa tgcaattgta ggtttttcat
     2281 tcgccttttc tttccatttc tgtattattg cttcatctct ttctggaata atcaatgctt
     2341 tctctaaatc acaaatatca taattatttc ctttcatttt aatatttgaa taatcaacat
     2401 tatcaaaaca accaaaataa tgatatggtg ataaaattcc catatctatg ccggtacgca
     2461 tttcatagtt tactataata ttgttattac ataattcaag aatatcctgt ctatcgcccc
     2521 tatatggggt agcagtaagg ccaagcagaa atgaatgttt tactttttca attaacttac
     2581 gataagtttt ggcagcggca tgatgaaatt cgtcaataat tagataatca aattgctcgc
     2641 ttgatattct attaatgtgt ctctgtaaaa gctgaattgt agctatgtta atagtagcta
     2701 gttctgatag agatgcttct gtctcatgta atcttacatc cgttttccta tatattgctt
     2761 caaattctga cactgctacg tctagaattt catgggtatg cgcaacatat aagactttct
     2821 ttgattgtgc tttcttactg tcttcagcag ctattcttgt tttgccacta cttggtggca
     2881 gaataattaa tcctgcacat tgacctattg cacgtgcctc ctccagtgcc tttagcgctt
     2941 cgttttgaaa aacattatga atacgattat gaggtgaata accacattta catctaccag
     3001 ctataatcct tctcgcatca cgaggacata aaaaaccata tgctataaac aatcttccta
     3061 aatcagttgc atcaaaaatt gcatacttac aattttgacg ttccgcagtc gtacaaaaat
     3121 gctcttttac agaatcaagt atgattccag tagctgcaaa tattgcaaat tttaacccag
     3181 atattttttc taggcgatat atctgatgtg caacgtgagt gggtcgaatt gttttaaatg
     3241 acttttcttt taaaataaat gcagccattc cttgttctct atttacggtt accctatgag
     3301 tgaatatatc tgcaatttca gcaggaccat gtggggtttc gttataatca taaattatac
     3361 ttcttatgaa tggttccaac tcttcttttt catctagatt ccttactgca cttaatattt
     3421 gatcatttat taatgatggt gagacttctc taacagcttt aaaacactca aattcttcaa
     3481 cttttattaa actccccata aaatcacctt cttatttagc tgacctcaga ggctctttca
     3541 tctaacggaa tcgtgtttcc gacgtccttc aaccttaaag taatgaccat taaaaagctg
     3601 ttttcttagg cttggatgag ctggttacga ttgcggttag gctgaagaat gtggtgcgcc
     3661 tgcctcaaag ccttgcccca cgctcccttg cggaaacata gggttaggcg aagtacccct
     3721 tcattccata cttacttgcc tttttatgat atgttggtct tccgccttct aaatatttaa
     3781 ttacttcttc cttaacaggg tctgattcca acgcaccaga aattactata gaacctgaaa
     3841 tagcatcgct ctcaacaata gtatatttgt cggtatcaga tgcaactgca agacaagaat
     3901 tatgtgtagt taaaataaac tgcctctttt cttttccctt tcttatcttt gtacacatat
     3961 catcccatac acttcttaca tccaatgaat cttcaggttg atcaataaca atagtaaaat
     4021 taccatcact taacgccatt attaacatag ctgtacattt ttgacctaca gaaatatccc
     4081 tgataagctc atacttttgt tcattcaatc tgaattttat ttccggctta tcttgtggat
     4141 gaactctata ttgcaatttg agtaattctt cgtaactgac ttgagataat gaataatccg
     4201 gcgcactttg agccatagtt ccggtaagtg agagccactg ttccgacgaa attgagccac
     4261 ccttccggaa ggtgagccat tatgttgttt ttcctttaga atgtagttat gcactagttt
     4321 cagtgtaaat acattcaaag ggaggtcatc aaatgaccaa atacagagaa atccttaggc
     4381 tctacagcca aggaatcagc caacgcagca ttgcactcag ctgcgagtgc tctcgcaaca
     4441 ctgtagcaaa agtagttgcc agagcaaaag aacttaatat tacgtggcca ttaagttctg
     4501 aaataaccga tggtgaattg gaaaaaaagc tgtttccgaa ttcacaacaa tccacgactg
     4561 ttcctagaaa ataccctgat ttagagtata ttcataagga actggctaaa aacggtgtca
     4621 cgctaagact tctttggagc gaatactgtg aagaatgtcg tcttagtaat gagcttccac
     4681 ttatgtattc gcagttctgc taccactacc agcagtttgc agagaaaaag cgtgctacta
     4741 tgcacattcc caggaaaccc ggtgagcaaa ttgaagttga ctgggccggg cagacagctg
     4801 cgattgttga tagagatact ggcgaaataa tttctgcata tgtatttgtt ggcgttctct
     4861 cttacagcca gtttgcttac gtagaagcat tcctatcaca ggatcaggaa agctggataa
     4921 gtgcccatgt gaacatgtac cgtttctttg ggggcgtaag ccgtatcctg ataccggaca
     4981 atctaaaaac cggagtagaa aaagtatcat ggtatacccc tgttattaat aaaatctatc
     5041 atgagatggc agagcactat gatactgcag taatccctgc cagggtgcgt aaacccaagg
     5101 ataaacccaa tgctgaagga acagtcggga ttatctcaac atggattatt gccgcactcc
     5161 gtaaacagaa attcttcagc cttcaggagc ttaatgtagc aataaaggaa aaacttcata
     5221 gtttcaacag taagccattc caaaagagag aaggtagcag aaccagcatc tttcttgagg
     5281 aagaaaaatc actgctgtta ccgctaccca ctaatccgta tgaactggcg gtctggaaac
     5341 aagccaccgt tcaatttaat tatcacataa ctgtggataa aatgcactac tcagttcctt
     5401 atgagtatat aaagtataag gtggatgtgc gggtaacacg taacgtaatt gaagtttttt
     5461 atcataacca tcgtatcagc tctcatccaa ggctatatgg acgtgctagc caatatagta
     5521 ccaatgacgc acatatgcct gaggaccatc aaaactattt gcaatggaac gcagaaagat
     5581 tcctttcctg ggctgagaaa attggcccta atacaaatat aacagtaaaa gcgatccttt
     5641 cttcccataa ggttgaacaa cagggataca gaagctgtat tgcgttacta aagctggcgg
     5701 ataagtattc tgttccaagg ttagagaccg cttgtaaaaa agcactttcc tatacgcctt
     5761 atcccagctt taaaagtgtc aagaccattc tttcaactgg tcaggataaa gtaaccgagg
     5821 aacagacagc aaaaacaaat gatgattctg ctaactacgg ctttacccgc ggagcagact
     5881 actatgggag gaaatagatt atgacaaatg aagcaaccat caataaacta cttgaaatgc
     5941 gtctcaccac tatggctgat gcttatcgtc gtcagctgca ggaacactcc ctggacaact
     6001 tttcattcga agagcgtcta ggactacttg tcgatataga atatacaagc cgtaaaaata
     6061 accgtctgaa aagactgata cacagtgccg gctttgacca gagctatgct tgtattgctg
     6121 atatcaatta cagtgccggc agaaaacttg ataagtctca gattgaacga ttggcatcct
     6181 gccaatacat agcagaaaaa cataatatca ttatcatggg agcttccggt gcaggtaaat
     6241 cttatctatc ctgtgctttt ggtattgaag catgtaagca gtattatacg gtcaagtata
     6301 tccgtctccc cgaactactt gccgatctag ctgtagccag aggagaaggc atttacaaaa
     6361 gggtaattaa ccaataccgt aaagtaaatc ttctaatact ggacgaatgg atgcttgtat
     6421 ctctgacaga aaccgaatca agagatctcc ttgaaatcat ccacgcccgc cataaaaggg
     6481 cttcaaccgt cttctgctcc caattctcac ccgcaggatg gcatggaaag ataggagaaa
     6541 caaccttggc ggatgcaatc cttgatagga ttgtgcatga ttcatatacc atcgaaatac
     6601 atggcagcga atctgaccgc tctatgaggg aggtatatgg aatacaaaat aaataatatc
     6661 cggtagtgtg gctcaagcat accggaacgg tggctcattt attccggacg ggtggctcac
     6721 cacccgccgg aatagtggct ccgccacacc ggtatacgca agataataaa aattcacata
     6781 atgcctgtaa cctttgaact tcaataccta ttttctttga taatggtttt agtctgtcta
     6841 attcatgaga cacttcatat ctgagcactg tcaaaataaa atcatatgga gttactttgc
     6901 tacaaagctc atcaatctcc gtgtctctaa gatatgatcc ccttttcaat gcacatagtt
     6961 tgtttttaaa ttcatcaata ttcgtggact catatatttt tatctgcaat cttccatttg
     7021 actctttttc aaatttttca catttacttt tacgctcatt tgaatattca ttatatatct
     7081 caaacaattg tttaataata ttatctcgtt gtttattgat attcttgaga ttacttgtct
     7141 tttttactag tatttcttct ctttttttaa ggtcttcact ctcttttagt acttttaatc
     7201 gttggttttc taatgctttc ctatctcctc cggcatttct tatgtgttcc tcatatttct
     7261 gtttctctcc taaatacatt atgttccatt ttttatattc agtctgcacg gaacgaattg
     7321 ctttctcaat atcctcaata gctttattta ataaatctat tacatagctt tttgattttt
     7381 tgataatatc gtcattcctc ataatcgctg ggtctttaac taacggttct ttaaaacttg
     7441 gtatgtctag attttcagct tctaattttt gtgaaattat atattctcta aacgtattca
     7501 aataactttc ttgaactttc aaagctctat ctttttgctc aaggagttta taattatcat
     7561 atattgggtc cgaaagtagt ttattcagct tttctaattc actattattc tttgatattt
     7621 gttttctaac atcatttaat atatgaactg atcttaatcc ttctgcaaat tggatatcta
     7681 attcagacaa atcgatttca gtattcttta ctctattttg ataatgttgg aagtcaaaaa
     7741 atctatcaat gaatcttatt tgttcatttt catcttcagc tatttttact atttcattct
     7801 gactcaggaa taggatttta aaggaactgg caatcctctc ttgttcttct ttattataag
     7861 gattattttt tgatggatta tatgttcttt caatttcatt ctctacacca gtctcatcta
     7921 taaatttaat tttgacagac ccatatgttt caagtttctt ttctaatttt cgtttatgat
     7981 catcaagaat ttctttttga gatgagggtt gtccaagtac aaatctcatt aattcaacta
     8041 atagagattt gccagaacct tttgctccaa ggatactatt tagtcctgaa tgaaaggttg
     8101 caacttgtcc atcaaaaaag ccacctttta cttcaattgc cttaatgtag ggataaaaat
     8161 cttctttata ctcaaagtac tgtctaattc ttacatctct atcaataaag cattgcctaa
     8221 ggctttccaa atcaatttca tcatcgactt taaaatatgt atatttagaa cctatagcat
     8281 ttaaattatg gttatcatca ttttctcctt ttgaatctga agcaatgtag acaccaagcc
     8341 tgcgaaagtt ataattttca tctttaccat caagtaagtc aatcgcacga atatgttttt
     8401 ttatcttttc cggatcaata aaatcatgat acgatgcctc aacggcaagt aatgccgggt
     8461 tttcaaatat tttagttcta gtaagcccag tgatatcatg aagaacacct tttgacgacg
     8521 tgcaatgagc taatactcct attccattac attccgatat aacatcaatt acctgatatg
     8581 gagagagtgt tgaaaccgca ttttttgtcc catactcatc gggatttatc ttcagtttag
     8641 ctaaaatggc ttcaatgtgt tttgtacctt tatccggacc caatatcccg ataacatgta
     8701 ttccatcttt tcctccggtg caatatattt cgactccagg gaatacaacc aaattagtgt
     8761 tattagcctc tttttttact ttgtctatcc aatcaccagt attgtgatct gtaattgcaa
     8821 taccttttaa tcccttttct attgtttttt caactatctg ttttggtgta acacttttat
     8881 caacaaaatc atgagaacca ggagtatgta catgtagatc aaatttttgg aatgataagc
     8941 ccgagtgttt gttcatctgt attacctcct tacttctttt tatggggata ttccgcctaa
     9001 ttcctctatt cccaaactta tcttctccgt ccccgcttcg ccagtgatgt aaatggatgt
     9061 gaaaccatag ctccaatata gacacttttt gttgttaaca tcatatcaaa ttcttatcag
     9121 acatcactat atacgcattt atatgattgt cacataatac atagttgtat atttctatat
     9181 tttcctacaa tttcctccta aaagccttag aataaaacat aaaaagtatt ccgaaaatat
     9241 tacatttccg gaatactttt tattcgccat ttccactatc agagcttatt attgaaatat
     9301 tcttttgtta cccccagctg cttcttaagt attattctaa aaaaattttt ctctatctct
     9361 cccgacccca ttgatacctt tgtccttcta atatttcctg aagcatcaat ttttctataa
     9421 aaataatggt cggttgtctt gtacaattcc cagccatcat tctcacaaaa ccttttcagc
     9481 tctttccagc ttggcatttt atcatactca atatatcttt tgtttcttcc tgcaggagta
     9541 ctttaaggac atagggcaga tgggcttttc tgttaggggc attgtaccag tattcaaatt
     9601 cattatagta ttcttcagca tattcctgta agtccgaagc caggatgtcc aatgcttctt
     9661 cttctgtact gccgtttgct gctatatcta tttcttcaag ctctgcagtt atagttccgt
     9721 tttcctcttc atatacagtg gctgtaagtg tgtattcctt cagcatttca ttcagcatat
     9781 cggtgcttgc agcaaagata taatctcttg agcgttttat aaattttggt ttgtttctga
     9841 caacggaatc aataaatctt ccccagtcct tccttacatc tgttgcgttt atcgcttcca
     9901 atttcttcat cccccttcgc ctcccattat aacatagaac gtacataatg tacagtatgt
     9961 acgttcctat cttataatat tacctgtatg ctgtatttta ttcctggtat tacaagattt
    10021 caatgagggt tttgatatat tcatctagtc aacactgttt ttgcctccta acacaataag
    10081 tgtacaatta aattccctct actataaaaa aaagagggct gccgccctcc ttcagctatc
    10141 tcttatgctt tatattgtcc atgtgctcct gcaccagcct cagagtttca ccgaacctct
    10201 ggaagtgaac cacttccctc tcccttaaga agctcagtgt ctctattact cctctgtcct
    10261 tggtggagtt gatcaaatgc tcgtaggttg ttcttgcctt ctgctcagct gccagatcct
    10321 catgaaggtt tgttatggga tcttcaattg cctgtatata tgctgccgtc cagggtactc
    10381 ctgcagcatc gtttggataa acatcacggc cgtgctgaat atagttgggg cccattcccg
    10441 cctcaatgag ttcttcaggt gttgctctgt caactatttt gaaaataagt gtcgcaatta
    10501 tctcccaatg agccagctct tcggtgccga tatcagtcag cactgccttt gcctccggta
    10561 taggcattgt atatctctgt gacaggtatc tcagaccggc tgacagttcg ctgtccgggc
    10621 cgccgtattg tgcaaaaagc agctttgcga tgtgcaggtc gcagccctct acccaagccg
    10681 ggaactgaag ctttttctca tatatccaca tatattatct ccctcccatc tcataatcaa
    10741 tttcccacgg ccagggatcc tctatccact cccagggaca atcgctaatg aatttatggg
    10801 ttatcagtgc gtattctttt tcatattttt ccttgagcat cctggatttc atcacatatt
    10861 cattatgcag cctgagtgct ctctgatcca ttggatgtgt atccagatac agattcagtt
    10921 ctaccagaga gaaatcaatc gccataattt ccttaagcag ttcaagcctg tctctgctta
    10981 tatccattac aaataccttc ctttcttcaa tcatctctgt cctttttcat ctttacatac
    11041 ggccggtaca attcgggaaa gattgtaccc ctcatcaagc ccttcatagg ctcataaacc
    11101 ttctgaagca cctggtaagg aatgtatgcg accgccagct tgaattcggg acccataaca
    11161 ggtatttcat agtgcggagc tacaagcatt tcaggtttgt acatatgatc ataggtatag
    11221 ccttttgcca tcattgaccc ccctttatac gttttccctt ccatactatt catctgcctg
    11281 ccgatatgtt acatgcagtg ggggctaatg caaaaaatat aaaactcctc caggcaaaaa
    11341 caaattcatg ccgtaaagga gcttcttgtt ctgtattttc tatcgattat tcctgtaaac
    11401 caaatcgcat ttttcttcta ttttaagctg ctcaaaatat ctgttttcca taggtatcca
    11461 cttggtcaaa aactgctcca gtattacttc accgtttctt tccagtatcc tgtccctttg
    11521 tgttttttca tcaattccaa ggaaaatttt taaatcatag taatctatta aagccggatg
    11581 catgctgtac gccccttcta ttatgttcag ctttttgggc aggacggtta tgtattcatc
    11641 cataaccccc ttcttgcagt catatatccg gtacttgaat tcactgcccc ttagtattcc
    11701 gtcaattact tcttccctga agcgttcata atccacattt cctccggcct cctccatcct
    11761 ttcccgggtt ccttgcccgg gcctcagata aaagtggtcc atatggaaga tgttgcagtc
    11821 gtatacgtca cctataagtg cggcaagggt gctttttccc gctccgcagt taccgtcaat
    11881 ggctgccagg actctgtcac gggatttcag cagggagtca atttttcgga atatctctaa
    11941 ataatgaata taatccagct tgactatacg gtatgccgga gaatataatg ccttgtattc
    12001 tccgctgtga tgcatcggcg gataaccccg ccttctatat tcatccagcc aggcttccgc
    12061 ttcctcaggc ttgtatggaa aatccccgtc agcacagcat tccttgaata ccttcagttt
    12121 ttgctcaaag ctttgaacat ttcctcttat agaatttgaa gtgctgataa agaaccggtt
    12181 caaggttcca atatcgatac cggagatttg ctccgacaca agataaaacc tgcaaagtcc
    12241 gtttcctata ttttcaaagg gaaccggcgg aatatatgca ccggagtctt tttcaagaat
    12301 ccggagttcc tccgtgagtc ttttaaggca ttcatcctca tttgtaatca agtgcccgcc
    12361 ggcaaattcg ttttgatata taagctttac catatccgca atctgcattc cgggatatct
    12421 ttcatgctgc atcagcaata ctttcttaaa ttcttcccta tcctgcattc tgcattacct
    12481 tagccttttt taaagctata ataattattg gaattaaaat caattgaatt attatgcccg
    12541 ggacagcgtt aataaacgca cctgccataa agacctccca ggtaaaagaa gtaccgccaa
    12601 gtccgtacag gaatactcca gcaagccccc aggctattct tccgcagatc atggatatca
    12661 gtaaagcagc atatatgtaa gcatctctct ttggtaacag cttatacata agccctgtca
    12721 tacagccata gacagccagc tcggcggcca tagcggcagc tgtcgggaac atgggcggca
    12781 tcccgaacag catacttctg aataccggca ctataaagcc gactatcaag ccgtattgcc
    12841 agccgcatac aaaacccgca atcagaacag gcaaatgcat aggcagaaat ttgctgccca
    12901 ggcttgggat ctgtgatgtc aggaatggca atacgaggcc aaaagcgata aaaaaccctg
    12961 aaagtacaag attcttgatt gataatcttt ccattttgtt ttcctcctgg tgaaaaaata
    13021 aaaagccatg caaataccgc tttcaatctt gaaagcaaat accttcatgg tataaatcct
    13081 aaaatatcat ccctgtttgt ttcagtaccg gcttctgccg gcgattaaac ttagtttagc
    13141 atatactgtc ctgctttaca agattcactt tcaaaacact aattccccag cttaatatta
    13201 tacaccctca gcttataatc cgaagggtat ttcagcatgg tcctgtataa tgagcgggta
    13261 ttcgaatcca tatcatgaga ggccagcact atatccctta tattgtactc gggatccctg
    13321 ttatatcctg ttacggccaa ggtatgatac ggagcacccc cggaatagca gaattgcacc
    13381 acatccccag gaaataccga gtcggacagc caatttatgc tttgcatcgc ttctgtatag
    13441 ctgagtattc tatgccttgc agcccggctg atccagtgag ccttaaaaac tgcagctatc
    13501 ttccatgaga aggttatccg gttctcgctg tctttagcag tcgcaccggg cttgcagtac
    13561 cagttgatac ttttcttatg atttgcaggg tttttatcat ccgcccaggg catccctcct
    13621 gcatacaaca cctgtgaaac aaaatttgta cagtctcccc ctccgtggga aaaggatgga
    13681 tatgcaggat tatagttttt ccagtatgca tctatataag cctttgccct ttccctgtcg
    13741 taaaagctgt ttgcagttct tgcatcaatg cttattccgc tgtcaatcat tgaatccccc
    13801 caaatcagcc cattgaatat taatgcccat aaattattat attcctgcaa atgggaaata
    13861 ataacctacg ggcatttcca tatagtcccc gaaatgccgt cagccataat cttaaaacct
    13921 gccatcagca ggtgggtgcc ctgattcctg cttcaatcct ctggtctgtg ccagcccgat
    13981 ttccacttta atacccgggc tccccattgt aaattgaggc ttaagattac taaaccaacg
    14041 aacatttcag gtacatttcc tgttacttaa attataaacc tgtccgtgta aaaaatcaat
    14101 gtgtcttcac cttgaataca ataattccat gaccggtaac actgctattc attctttttc
    14161 ttcagcaacc tgtagtattc agtcagttgt tcatccaaga gcctgctgag ttcgattatc
    14221 ttactatcca acaggtcatc actttcgtat atcatatcat tcatcttttt tctaagctcc
    14281 tcgattttca tcagcacttc attaatttct tccatttgta tacacctttc aactgtatta
    14341 atatcatgtt tgtgagtata gcgaactatt gtgacatatt cagtatatta atccgtataa
    14401 atacttaata aacagagaaa actggaagca tccgaaaatg ctcccatttc aattcaaagg
    14461 ttcaaaatct gcataatctt tgaagttcat cagtcttgct ctatggactc catctccctg
    14521 atgtttatgt tgcagtttaa tccgatacca atctcttttc gccggatatt tcctgaatcg
    14581 gttgtatgtc ctgggtcacc tccatagtgc cggcataatc cccattatca tcccttatcg
    14641 cgaaatacct tatgtatacg tacctgccgc ccatcttaat ccagaagctt tcgctgtctt
    14701 ttttacccgt cttgaagtcc tccaccagct tttcaacaat atggacactt gccggaggat
    14761 ggcagttatg cacctgcctc cctatgattg ccttagtcct ggggaatatc ctttcttttc
    14821 cctgagagaa gtatttaacc gctccgtcct tatctacaaa ggttatatca atgggaaggc
    14881 tattgaacat aagactgatt tccttagctg tcaaaaggcc tgtttcaaat tttaaataac
    14941 cgtcggcagc tgtctgtccc gtttctttct gcttttcttc cacatctgtc ctctcgggct
    15001 tccacttgcc ccggggttct gtcaggcagt agcctatctc atcactttca ctctcaatgg
    15061 tcatccactc gtcctccgtt aaggtctcca ataccatcgg gaaaagtata ttctcttcct
    15121 tgaatatcat ttctatcact ctgcccgctg cctcatccgc ttttgtgtat acctgctcct
    15181 tctctccctt ataatcttgc agcattccct taatttcttt tattgctgcc ctgatctcat
    15241 catccacacc ccacattact ttggggggag ccgttatgcc gtacttctcc atatagggga
    15301 acagcagatt ctccttcctg ctgtaatgct tatcgatatc aagaagcagg ttgatatcct
    15361 ccagcagctt gtatatgttt tcactgttgt cggaagccct gaatcccgcc aaatggggct
    15421 taagcctctc atttatgagc ttttccagtt ctctgttttc aagcttgaag gtatgcaccg
    15481 gatgtcccgg tgtttcatcc ggtttccgca ccttgtggat atcctcaatg gagcctttga
    15541 agactgccgc atgcacatca caaagccttt gtatctcttc cacaggcatg ccttccataa
    15601 tcagcgcctg ctccatctcc gatatctctg aagccgatac gccctttatc aactcttcaa
    15661 accttgattt tacctcttcc accgtctttc ctgcatggag ttccataata agttccttca
    15721 gaaccttctg cctgtattcc ctgttgttta taatttcact catttttaat gccccctatc
    15781 cttttattga atatccctta ctctcaaaag cttgacttat tttttccata cttatgccct
    15841 tcattgcagc acctttggga atagtcataa aacggcctgc ggtattcagc atgccctcat
    15901 tggtgatgct ctcaaaaccc agttccttca tgatgtccac tatttccgga tattcccgtg
    15961 aaagctcata gacagtcttg ctaaggtcta tttccccgga catatagaca tttccccttt
    16021 caattattca atttggatat tctgtgcatt ttatgagaat gtcattttgt tttccttaat
    16081 ctgcgtccag cataagcctt tctatctcca agggcgcaac aggagaaccg tttttgtatg
    16141 ctctcatatt ccctcccgat atttcatcaa tgagggtgat ttgcttatcc tccccggttc
    16201 ttccgaattc gaatttgata tcatatagct ccaatccctt tttagccagc tcatccttga
    16261 ttattcccgc aattttttgg gtcaggtcct ttaatacctt atattcatcc ttggataaaa
    16321 tccccagcat ttccaacgca tcttctgtta tcggcgggtc ctccctctca tcatccttta
    16381 gggtaacctc cacaaaggca tccaggtcct gtccttcctc aacatacttc ccataacgcc
    16441 tcaaaaagct tcccaccgct ttaaaacggc atacaaactc caaacctttt ccgaataccg
    16501 aagccttttt tacagtcata gtacctgcct caatatctgc gtctatgtag tgtgtcggaa
    16561 ttcccaggct gttcagcttc tcatagaagt atttggtcag cctgaggccg gccctgcctg
    16621 ccccctcaat agtaaggcca acggtgttgg agccggggtc gaacacacca tctgttccgg
    16681 tgacatcatc cttgaatttc agcaggtaat ttccatcttc caaagcatat acgtttttgg
    16741 ttttccctgt ataaacaatc ttcataagca agttcgttcc tttcttatac taatttcaca
    16801 gttgaagttc cgatgttatg attaaaaagt atatcaaatc attgaaggag gcacaagaaa
    16861 aatatcttat cagctcacta atatatgctc ccttgactat atatagcttg taagatatca
    16921 tcaaaaatat acaggatttc ctgaattgga agggaggtat ataattgaag cttaaagtat
    16981 tatggacaat ggcagagggc atgggaaccg cctctgattt ggaaatgctt aaaaatgcca
    17041 agcctttcga tatagatgtg gttactgcag gtacaaaagg caaagatgct gcaggctttg
    17101 taataacggg taaaaaattc gtggttcctg aagtaaagga ctttaaatat ttaaatgcca
    17161 tactggacat atgcaggatc gaaaaggtca ctaccgttat accccaatac actgacgaac
    17221 ttttgctgat gtctgagaat acaaagcagt tcaatgattt aggcataaag gtactggtta
    17281 ctgaagacgc cgataagctt agaatcgcaa acagcaagag tgaactatac aaatatttca
    17341 ggcaaaaaag cttcgtaccg gaatacacat tggcttctga tatcgattca ataaaaaaag
    17401 ccgtgtacga actgggatac ccttctgtgc ctgtatgtat aaagccggta caaggggagg
    17461 ggggaaaagg attcagaatc ataaccgatg aaaaaatcga tatattcaag gagcccgtta
    17521 attctccgaa gcttagcctt gaagcatatt tggaaagtat aaaaatttcg gacagcattc
    17581 ctgagctttt ggttaccgag tatcttccgg gaaaagaata cagtgttgat tgtgtatgca
    17641 aaaacggaac aacgtatatt tgcataccca gacagagaat agaaactgca atgggagtat
    17701 ccagtgtatc cttgattgag aaaaatgagg aattaatttc atattcaaag gagataatat
    17761 cctccctcag cctttcatat aacattaata tacaattcaa gtattcaagc gaaggaaaac
    17821 cgaagctcat tgaaatcaac cccagagtat ccggttcttt agttgcaaac tacggggcag
    17881 ggataaatat gctggaagct gctcttagac ttgcttatga tatgcccttg gaaaatattg
    17941 aaataaaatg gggaacaaaa atgataaggc aatggagaga gattttcata cagcagcacc
    18001 ctaaaccatg attgccttat acttcttcag catagtgcag ggattgtatt tttctttgaa
    18061 tcgtctgagg ccgggaagcc ccatgtcatc ctctttattt atatattcct catccatcct
    18121 cttcgccagc tcgcttacaa gatatgtatg gacacctctg tgggaattgt tggtaagctc
    18181 aacatgggat tgggccatgt cgccggatac tttctcatac aggcaaaatg ccgccacttc
    18241 atcattttcc aggactactc cgcccttcaa attgaattgg gcgatgctgt ccagcatctt
    18301 atatgtcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc catatcagac
    18421 cggattatta ctgctccgtt gctttctgct attttcagct tatacaagtt attatacata
    18481 tatagatatg agaactggta atccgagcaa atgtatccaa acttgccgaa aatatgctta
    18541 tcgtcaagtg tgatttccct gaagtccata tgcttctgcc tcctttagaa aatgcagagc
    18601 ctccgcagaa aagggcttcc ttaaaggcag cccctattcc cgacatgcat ttgttcttaa
    18661 ccggcaccaa gcttttttcg acctgattcc cggcttctac agttcgatat gtgctgcaat
    18721 gccatatccc ttatttcctg ccttgggccc tacgaaactc cattttcccg gaattccaag
    18781 atctttggcc aacccctcca tagtatacat cattatgccc gcatcaatat aatttcttgg
    18841 attctgaggc tccagaaccg tcagttccgc ttctccgtcc ttcaatatta ttctgcaagg
    18901 ctgcaaattt ttataagacg gcgcattcct tatatagaag aacaggtctg ccatacctct
    18961 gctctccaat tcctcatagg ccgccggcct accccattca tgcaaataaa caatttcacc
    19021 caaggcaagt ctcgccctgt cggtaatctc ggcatccgca tattttatgc cataaggatt
    19081 ctgcctgtat tccgaagctt catttgttac ggatgtgcgg ggttttttat tctttattgc
    19141 cttctcatcg ggtattccga atgcaagaag gtaattgatc cttgattcac tgtctcccga
    19201 cagttttgcc tttatatcgc cggatacatc cctgatatca atccagcagc tgcccagata
    19261 catctcctgc agttttttga ctaccgactg catatgatac gcgccgaaga attcatcttc
    19321 gaaattttct ttggtaacgg caagccctat ataatgcggg cttttaatca taacaccgct
    19381 gtaaccgccg acacctttca atgcattaaa gacatcactg ccgtttttca gcatgataag
    19441 tgaaaagcct ttttctttcc ctgcatcggc attgattttt tcaaggtata ctctgatatc
    19501 tgaaagcacc aggtccttta aattctcttc ctcataatcc cttacagatt gaaggtttgc
    19561 caggaaatct ctaaagttca taatcatgcc tcctttacta actctgttta ttatatctta
    19621 attcatagtt caataaatat caagtaatag tgtttattgt ggg
//
LOCUS       DAOOAB010000050        19533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3716_length_19533_cov_15.060581, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000050 DAOOAB010000000
VERSION     DAOOAB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19533
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3716_length_19533_cov_15.060581"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..2002)
                     /gene="selB"
                     /locus_tag="PKU88_10015"
     CDS             complement(92..2002)
                     /gene="selB"
                     /locus_tag="PKU88_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPX69648.1"
                     /translation="MKNVIIGTAGHIDHGKTTLIKALTGRDTDRLKEERERGISIELG
                     FTYFDLPSGRRAGIIDVPGHERFIKNMLAGAGGIDAVILVIAADEGVMPQTREHLNIL
                     SLLQVKKGIIALTKKDMVDQEWLEMIIEQVREETAGTFLEDASIIPVSSISGEGLKEL
                     IAEIDSVTEIVEEKDAHKIFRLPADRAFTITGFGTVVTGTLISGSINEGDRIEIYPRK
                     TETRVRSIQVHEKNVKTAYAGQRVAINIANIKLDEIRRGDILAQPGCMEPTMMLDARL
                     EILKDADKIIDNRDRLRFYQGTSEIMCRVVLLDREELKPGESAFVQLRLEEETACMKG
                     DRFVIRTYSPMLTIGGGTVLEPNPPKRKRFKEEVIDELKIKEQGNPTEVVERYLIQNS
                     DKFPEKKTVLKAAGNMTAELYDKIAAELLEKKLIYEFRLGDETYILHMKYLRDVETKL
                     TELLESYHRRNPLKSGISKEELRTKVFEGMKPRLADMVFGYFEDGGIIRLENQYTALS
                     GFKVQFSKQQEHIRDTILQEYKKNRFNPPRLSELAAAKRLNINQCQEVYIALIEMGEL
                     VKLDEDIAFSKYAYNEAAELLRKYIKDNGSIQLGQFRDLLGTSRKYAMALLDYFDQNK
                     ITKRIGDNRVLF"
     gene            complement(1999..3447)
                     /gene="selA"
                     /locus_tag="PKU88_10020"
     CDS             complement(1999..3447)
                     /gene="selA"
                     /locus_tag="PKU88_10020"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPX69649.1"
                     /translation="MIDKKSILSSIPSVDELMKDKELERLTVLYSRTAVVEGIREYLR
                     EYRKHVMSAKKDEELDLANGSDIMKGIIEKIHNMMRNNLVNVVNATGVVLHTNLGRSP
                     LSSRLKDTLWNIASRYSNLEMDLEAGERGSRYSHVEGLICKLTGAEAAMVVNNNAAAV
                     MLVLSTVAKGREVVVSRGQLVEIGGSFRVPDVMKQSGAALVEVGTTNKTHLEDYESAV
                     NENTAALMKVHTSNYRILGFTSQVEPEELVVLGRKLGLPVIEDLGSGLLIDLTEYGLP
                     YEPTVQQAVKAGMDVVTFSGDKMLGGPQTGIIVGKREYIDKMKENPLTRAMRIDKLTL
                     AALEGTLKLYTDKELALAEITVLRMLTYDNEELGKKARRLGVRLKKTIGDMAAIGIED
                     EFSEVGGGSMPLHKMPTKVVAIKPKTVSLTGLEEKLRAYKTPVIARIVRDRLLLDIRT
                     IMDDEYKVVENAMLWAFTQLSGSKLGEGEGQA"
     gene            complement(3516..4562)
                     /gene="selD"
                     /locus_tag="PKU88_10025"
     CDS             complement(3516..4562)
                     /gene="selD"
                     /locus_tag="PKU88_10025"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4509..4511),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPX69650.1"
                     /translation="MDSKNKTRLTQMTKSSGUAAKIGPETLAQVLCQLPKFYDENLIV
                     GIDTSDDAAVYKIDEDKAVILTMDFFTPIVDDPYTFGQIAAANSLSDIYAMGGRPVTA
                     MNIVCFPTCLPIDILKEILKGGADKVKEAGAIVVGGHSVEDDEPKFGLSVMGMVHPGK
                     VTANSGAKPGDMLILTKPLGIGILNTAIKADITDQEAYKKAVDVMSYLNKDACEAMMK
                     VGINGCTDITGFGLLGHGYEMAEASNVSLEIWSDYIPIIKESIDFAKMGIIPAGAYNN
                     EGFIGEHIRFDKSIKQEVRDIMYDPQTSGGLLISVPEQNSEILMEELRKNNKTDFNII
                     GKVKTKGKYSIEVI"
     gene            complement(5030..5644)
                     /locus_tag="PKU88_10030"
     CDS             complement(5030..5644)
                     /locus_tag="PKU88_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPX69651.1"
                     /translation="MLYITRTQKSILAFVILVLVIISGTIYIRQQKATEISLAEAVPD
                     QAEAQAETKPAEISVYIYGFVKNPGVVKVLEGTRLDEAIRLVGGAVEEADLEAVNLAY
                     RLADEDAIYIPKKGESPQDTGKAIPGVNTVKSAAVNKPGKVNINTAGEKELDTLKGVG
                     PATAKAIIEYREQKGLFNTIEDIKKVSGIGEAKFNNMKDKITVE"
     gene            5980..7200
                     /locus_tag="PKU88_10035"
     CDS             5980..7200
                     /locus_tag="PKU88_10035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPX69652.1"
                     /translation="MKRLIAYIMILSLMMPFVRVYGDVPEVTAPSAILIDAETGKILY
                     EKDADEKRYPASTTKVMTGLLAVEHGKLDEIVTIGKNPPLVEIGSSQIYLVPGEQLTM
                     EQLLYALMLESANDAAVAIAEHISGSVEEFAKLMNSKARELGAASTNFVNPNGLHNDN
                     HYTTARDLAMIAKYAMTLDKFRDVVKEVKYTIPKTNKQEERNYITNSNKLIWEIYDQF
                     RYEYATGIKTGYTTKAKQCLVGGAKKGDLELISVVMGAEGQNMYSDTVALFEYGFSNF
                     QKVQILKKDQIVTSVPIKEGQEKINLLAAEDFSMVLSESEKENITTEIKTRDGLREPI
                     EKGEVLGEIIVYAEGNEVTKIELLSSVSVEAVKKPILWWLWAIAGFLLYRTVVTIYKV
                     RRRKKYRNVTYIKR"
     gene            complement(7332..7715)
                     /locus_tag="PKU88_10040"
     CDS             complement(7332..7715)
                     /locus_tag="PKU88_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPX69653.1"
                     /translation="MTKEIISAEKAPGAIGPYSQAVKYGNMVFTSGAIGVDPATGVFP
                     EGGIQVQAKQCLENLKAVLEASGSSLENVLKTTVFIKDMNDFSKINEIYGQYFTEKHP
                     GRSCVEVARLPKDALIEIEAVAYIG"
     gene            complement(7737..8387)
                     /locus_tag="PKU88_10045"
     CDS             complement(7737..8387)
                     /locus_tag="PKU88_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPX69654.1"
                     /translation="MSKDLHPILKGIIPLIDGIANTFGKNCEVVLHDIRNPQSSVIAI
                     ANGHITGRSIGSPMTEYGLATLRSGQFDKPIVNYRKKTRDGRILKSSSLFIKDENGKL
                     IGFLCINYDISELTIARNIINNLANVIEETDFSEEDECFGNTVNEMLSSIVNKALESV
                     GKPVAFISKEEKVNIVQLLDEKGVFLIKGAIDYVAKVLCVSRYTVYNYLDETRVND"
     gene            complement(8420..8752)
                     /gene="rsfS"
                     /locus_tag="PKU88_10050"
     CDS             complement(8420..8752)
                     /gene="rsfS"
                     /locus_tag="PKU88_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPX69655.1"
                     /translation="MANKAIELLKGKKAKDIRLLDIHELTTISDYFVIAAGSSTTQVQ
                     AMSDELEEKMELAGYEMYHKEGFRNGRWILLDYGNVIVHLFHDEERQFYNLERLWVDA
                     KTVLVDNE"
     gene            complement(8850..9827)
                     /locus_tag="PKU88_10055"
     CDS             complement(8850..9827)
                     /locus_tag="PKU88_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPX69656.1"
                     /translation="MKKYIIIFLASFLVFFAIIGGVYNKYKSNMTDEHFVRTIEENPE
                     LEPEDTKEDVIINTLVMGIDEARSDTMIVVSYNKENQKIALLSIPRDTRVEIPGYGID
                     KINAAAAKKEGTALAMRTVGNLLDVPIHHYVKVTFKGAERIIDILGGVSVNVPPGMDY
                     EDPVQDLYIHLKPGKQVLNGKDAVKFARFRSGYPDQDLGRIRAQQQLIKAFIDKLTSP
                     KMIPKALTIVDAMSKCIKTNLESSDIAHYAINIKDIKMENVSFYTLPGSDGYINRVSY
                     FLHDEAKLKEMIQQIRIDLGVEEPAEEMELLPADNDNAPEGAESGIGLR"
     gene            complement(9878..10480)
                     /gene="yqeK"
                     /locus_tag="PKU88_10060"
     CDS             complement(9878..10480)
                     /gene="yqeK"
                     /locus_tag="PKU88_10060"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPX69657.1"
                     /translation="MLDLNSIMEHLEKTISSKRYIHSVNVSKTAKKLAEFYEYDAVKA
                     EIAGLVHDCARDLEKPLQLKCLKEEGIEADELTLNIEELLHGPAAVHICRKVFAIEDE
                     EILSAVRYHTTGKENMSLLEKIIYLSDFIEPSRSFPGVEELRGLAFKNLDKALLLAFN
                     SSILYILSKNEYVHINTILARNHVLKEVGENGMQYHDSSR"
     gene            complement(10486..11121)
                     /gene="nadD"
                     /locus_tag="PKU88_10065"
     CDS             complement(10486..11121)
                     /gene="nadD"
                     /locus_tag="PKU88_10065"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPX69658.1"
                     /translation="MYEDAVDNGQSRIGIMGGTFDPIHYGHLAAAEAARIEFGLCKVI
                     FMPVGNPPHKQSQAISDAEHRYRMTALATSSNSGFEVSRLEVDKAGITYTFDTMKELR
                     NIYGEAPAIYFITGADAVLELLTWYKLGELLTLCKFIAVTRPGFDICKLEQKIAEITS
                     KYDGEIICLEVPLLEISSTDIRERIRSGKPVKYLLPEEVEAYIHKNGLYKE"
     gene            complement(11181..12479)
                     /gene="aroE"
                     /locus_tag="PKU88_10070"
     CDS             complement(11181..12479)
                     /gene="aroE"
                     /locus_tag="PKU88_10070"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPX69659.1"
                     /translation="MMNNVFGLIGESLRHSFSPQIHCEIFKMLGIEGEYRLFELQEEE
                     LEPALLKYREQGIKGLNVTVPYKIKIMPYLDVISEEAEKIGAVNTISLANDVLTGYNT
                     DYRGFGMLLARSNIEVKNKTAVILGTGGVSKAAAQYLMDNGVKRITYVSRNPGDGAVG
                     YDETESLKGDIIINCTPCGMYPKADVSPVPYGTFKQFGTAVDLIYNPEKTMFLKQAEE
                     NGLKAVNGLYMLVGQAVAAQEIWNGIGLSRSQVDNIYQHIKKMLYKADTSKNIVLIGL
                     SGCGKTSIGRLLAEKLERKFIDIDDMIEKQEGCTINELFRQGEEHFRNLETKAVLALE
                     REKALVISTGGGVVKRAVNMKSLKKNGIIIFIDRDVCDIMDDIDTSTRPLLAGGTGRL
                     LQLHAERYDLYKEYSDFTVCNDSKAADIIEDIYRKLVEKL"
     gene            complement(12497..13609)
                     /locus_tag="PKU88_10075"
     CDS             complement(12497..13609)
                     /locus_tag="PKU88_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPX69660.1"
                     /translation="MDELEILRQKINETDEKLVKLFIERMALAKKVGEYKSGKNIRIY
                     DPAREKDIVGRFVNRVRVDFDIKYIEQFLENLMFLSRRLQGDIIGRKQEPSKIENLNT
                     NMAIGYQGVPGCYSYQAGLEYFGEGIEALSYHSFRDVFDALAKGEIQYGILPIENTTS
                     GSINEVYDLLREYEFYIVGEKCLRVEHSLLAVEGAKLSDIKEIYSHRQGLIQCSRYLS
                     QYPEWKQIPYFDTAGSAEYIAKEGLKSKACIAGEKAASVYGLCIICRGIQDNRNNSTR
                     FVIISKEKLDNEKADKISIVFSVPHKPGSLYGILRYFAEGNCNLMKIESRPLEDKAWE
                     YFFYTDFSGNINEEGIRGILADMEKECSYFRLLGNY"
     gene            complement(13632..14720)
                     /gene="aroC"
                     /locus_tag="PKU88_10080"
     CDS             complement(13632..14720)
                     /gene="aroC"
                     /locus_tag="PKU88_10080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPX69661.1"
                     /translation="MSGIWGNSLKISIFGESHGKAIGINIDGLKAGILLDLDFIRREM
                     QRRVPGNSEFATPRKEGDEFEILSGFFEGRTTGTPLCAVIHNTDKKSEDYEELKNIMR
                     PSHSDYTGYVKHGGFNDYRGGGHFSGRLTAPLVFAGAICKQLLESRGITIGSHIRSIG
                     EEQDRPFNPVSVDSGTLKKLAGKAFPVLDAKSEALMKEVIARAKEQKNSIGGVVETAV
                     LGVKPGIGEPFFDSVESTLAHLIFSIPGVKGLEFGAGFDISKLMGSEANDEYYMDGEE
                     VKTYSNNNGGILGGITDGMPIIFRTAIKPAASIGRRQRAIDIEKKENVGLEVQGRHDP
                     CIVPRAVPVVEAVTAMALLDLIMVSQGK"
     gene            complement(14701..15978)
                     /gene="aroA"
                     /locus_tag="PKU88_10085"
     CDS             complement(14701..15978)
                     /gene="aroA"
                     /locus_tag="PKU88_10085"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPX69662.1"
                     /translation="MKEVKIYPGSLAGEVFIPASKSISHRAVICAGLSEGISNIYNVG
                     ISQDIEATIEALRSFGTMIEKADASLRIKGSAVPKLKNSGINCHESGSALRFIIPIAA
                     LTGEPVTFYGEGRLVERPLLQYYNIFEKQGIKYETEAGKLPLHINGRLVPGEFQLEGN
                     ISSQFISGLMFALPLLEGDSLITVTTEPESKPYIDLTLKVLKDFSIHIENHGYRTFII
                     KGRQKYKATDYTVPGDFSQAAFWLAAGALGSEVVCKGLDINSMQGDRVMMDIIGEMGG
                     GIFIKAAAVRALPADTRGTVIDASQCPDLVPILAVLGALSKGRTEIVNAGRLRLKESD
                     RLKAVSSELGKLGACIMELHDSLVIDGRKNLRGGRVESWNDHRITMALAIAATRCEEP
                     VIISNASCVKKSYPDFWVHYKALGGKIDERYMG"
     gene            complement(15981..17075)
                     /gene="aroB"
                     /locus_tag="PKU88_10090"
     CDS             complement(15981..17075)
                     /gene="aroB"
                     /locus_tag="PKU88_10090"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPX69663.1"
                     /translation="MVTIEVSIKSSSKKYNIQIGKNIRQELYKYLSERYRGKKAVIVT
                     DDNVNKLYGASLYRGIVEAGMKCRLISFSPGEKSKSLQVLSGIYEDLAGFNADRGDII
                     IAFGGGVAGDLAGFAAATYLRGISYIQLPTTLLAQVDSSVGGKTAIDLSSGKNLAGSF
                     YHPEIVFIDTLFLNTLEDRYLRDGMAEVIKYGCIKDKSLFDRLSDYENLQQCIADMDE
                     LVCTCCSIKRAVVEADELDRGERMLLNFGHTFGHAVEQYYNYEKYTHGEAVAMGMAEI
                     TKRSEEMGLTERGTAKALADILKKYGLPYEIPVANKKDLINVMRRDKKFSGDSIRLVL
                     LNKLGTAFIMKAKSEELESFLDTTGDARED"
     gene            complement(17076..17933)
                     /locus_tag="PKU88_10095"
     CDS             complement(17076..17933)
                     /locus_tag="PKU88_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPX69664.1"
                     /translation="MEEGDFRRNIAVVGLGLIGGSFAMALRKHNAVRIFGIDIDESVL
                     NKAAGCGVIDEGASNAKKILCKSDMVIIALYPREAIGFIKNYAEYFKPGTIVTDTCSI
                     KQPIIEAAKKSLRQDVEFVGGHPMAGNEFQGFEAASRELFFDTNYIITPHESNSENGI
                     IAVEKLAASIGCKCLTRIGAEAHDRLISFTSQLPHIVAISFANLMMREKGISPFTGRS
                     FKDATRVAALNKELWTQILRMNNLNIIDKIEDMEDMLHKLKDAIKEQDESSLQILFDN
                     AIKSKREII"
     gene            complement(18002..19015)
                     /gene="aroF"
                     /locus_tag="PKU88_10100"
     CDS             complement(18002..19015)
                     /gene="aroF"
                     /locus_tag="PKU88_10100"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPX69665.1"
                     /translation="MVIVMRENASDEEIEKLRERIETEDIKVSVIKGRDYCVLGIIGD
                     TTGIDISEIEANESVEAVMKIQQRYKLSNRMFHKDNSVIQVGDKTIGGQELAVIAGPC
                     SVESEEQILELAKEVKEYGAGFLRGGAFKPRTSPYSFQGMKLEGLELLKLARKETGLP
                     IVTEIMSVDMIERFVEDVDVIQVGARNMQNFELLKEIGKTTKPILLKRGLSATIEELL
                     MSAEYIMSEGNSNVILCERGIRTFETYTRNTLDISAILAIKKLSHLPVIVDPSHATGL
                     WWMVEPLAKASVVAGADGIMVEVHSDPANAKCDGQQSIKPEKFRAMMKDITLMADSLK
                     KAN"
     gene            complement(19253..19402)
                     /locus_tag="PKU88_10105"
     CDS             complement(19253..19402)
                     /locus_tag="PKU88_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69666.1"
                     /translation="MNNLLGKGISGIYYFSFNLGYYFSAGYYACKKEYIPFPDELEEF
                     KNYCK"
BASE COUNT         4955 a         4998 c         3349 g         6231 t
ORIGIN      
        1 agttatataa gtataacacc gataccattt aatgtcaacg gttattttca cttcgcagaa
       61 acgccaagag ggctgaaacc cagtcctctt tttagaacaa tactctgtta tctccaatac
      121 gcttggtaat cttattctga tcaaaataat ccagtagtgc catagcatat tttctgctgg
      181 tacccagcaa atccctgaat tggccaagct gtatcgaacc gttgtctttt atatacttcc
      241 ttaaaagctc tgctgcttca ttataagcat actttgaaaa agcaatatcc tcatccagct
      301 tcacaagctc gcccatttca atcagagcaa tgtatacctc ctggcattgg tttatgttca
      361 aacgctttgc tgccgccagt tctgaaagcc tgggagggtt gaaccggttc tttttatatt
      421 cctgcaggat agtatctcta atatgctcct gctgtttgct gaattgcacc ttaaaaccgc
      481 ttagtgcggt atattgattc tcaagcctta taattccgcc atcttcaaaa tatccgaaaa
      541 ccatatcagc cagtcttggc ttcatacctt caaaaacctt ggttcgaagc tcttcctttg
      601 atattccgct tttcagcgga tttctcctgt gatatgactc cagaagctct gtaagcttgg
      661 tctccacgtc cctcagatac ttcatatgaa gtatataagt ctcatcacca agcctgaact
      721 cgtaaataag ctttttctcc agaagctcgg cggctatttt gtcataaagc tcggcagtca
      781 tgttccctgc tgcctttaat accgttttct tttcgggaaa tttatcgctg ttctgtatca
      841 gatacctctc aactacctcg gttggatttc cctgctcttt aatcttcagc tcatcaatta
      901 cttcctcttt gaagcgcttt ctcttgggag gattgggttc cagcaccgtc cctcctccta
      961 tggtaagcat tggagaatat gtcctgatta caaaacggtc tcccttcatg caggcagtct
     1021 cctcctccag cctgagctgc acgaatgcac tctcccccgg cttcaattct tccctgtcca
     1081 gaagcactac tctacacatt atctcagagg ttccttgata aaatcttaac ctatccctat
     1141 tgtcaataat cttatccgca tccttcaata tttccaacct tgcatccagc atcatcgtag
     1201 gttccataca cccgggctgt gctaaaatat ctccccttcg gatttcatca agctttatat
     1261 ttgctatatt tatagccacc cgctgtcctg catatgcagt ctttacattt ttttcatgca
     1321 cctgtatgga acgtaccctt gtctcggttt tacggggata tatctcaatc ctgtccccct
     1381 catttatgct gcccgaaata agcgtacctg tcactacggt accgaagcct gttatggtga
     1441 aggccctgtc tgccggaagc ctgaatatct tatgagcatc cttctcttcc acgatttcag
     1501 tcaccgaatc tatctctgca ataagctcct tcagcccttc accgctgatt gatgatacgg
     1561 gtattatact tgcgtcttca aggaaagtgc ctgcagtctc ttccctgacc tgttcaatta
     1621 tcatttccag ccattcctga tccaccatgt cctttttggt aagagctata atgcccttct
     1681 ttacctgcag cagcgaaagt atgttgagat gctccctggt ctgcggcatt acaccctcat
     1741 ccgcagctat cacaagtatg actgcatcaa ttcctccggc acctgcaagc atgtttttta
     1801 taaatctctc atggccggga acgtcaataa ttccggctct tcttccgctt ggaaggtcaa
     1861 agtacgtaaa gcccagctca atggatattc ctctttccct ttcttctttc agcctgtcgg
     1921 tatcccttcc cgtcaaagct tttatgagag ttgtctttcc gtggtctata tgccctgctg
     1981 ttcctattat cacattcttc aagcctgacc ctcgccttcc cccaatttac tcccggatag
     2041 ctgggtaaac gcccataaca ttgcattttc cacaacctta tattcatcat ccattattgt
     2101 cctgatgtca agcagcagcc tatccctgac tatccttgct atcaccggag tcttataagc
     2161 cctcagcttt tcttccagtc ccgtcaggga tactgtcttt ggctttattg caacaacctt
     2221 tgtaggcatt ttatgcaaag gcattgagcc tccgcccact tcggaaaact catcctcaat
     2281 tcctattgct gccatatcgc ctatggtttt tttcagcctt acaccaaggc gcctggcttt
     2341 tttgcccaac tcctcgttat cataggtaag cattcgaagt acagtaattt ctgctaaagc
     2401 aagctcttta tcagtataaa gcttcagagt cccttcaaga gcagctaaag taagcttgtc
     2461 aatcctcatt gctcttgtaa gaggattctc cttcatcttg tctatatatt ccctcttgcc
     2521 tacaattata ccggtttgag ggcctccaag cattttatcc ccgctgaagg tcaccacatc
     2581 catgcctgct ttgacagcct gctgcactgt cggctcgtag ggcagtccat attctgtcag
     2641 atcaataaga agcccgcttc ccaggtcttc tattacggga agtcccagct ttctgcctaa
     2701 aactaccaat tcctccggct ccacctggct cgtaaagccc agtatcctgt aattactggt
     2761 atgtaccttc ataagagctg cagtgttctc attaactgcg ctttcatagt cttcaagatg
     2821 tgtcttattg gtagtaccca cctcaaccag ggcggctccg ctttgcttca ttacgtcagg
     2881 caccctgaag gagcctccta tttcaacaag ctgccctctg gacaccacta cttcccggcc
     2941 tttggctaca gtactcagaa caagcattac cgccgcagca ttgttgttta caaccattgc
     3001 tgcctccgct cccgtcagct tgcatatcag tccttccaca tgagagtatc tcgagcccct
     3061 ctcgcccgct tccaagtcca tttccaggtt ggaatacctt gaagcgatat tccacagggt
     3121 atccttcagc ctgctgctga ggggtgatct tcccaaattt gtatgaagca caacccctgt
     3181 tgcattaaca acattgacaa gattattcct catcatatta tggatttttt ctattatacc
     3241 cttcataata tcggagccat ttgctaaatc caattcttcg tcttttttgg cggacatgac
     3301 atgctttctg tattccctca ggtattccct tataccttct accacggcag ttcttgaata
     3361 cagtacggtc agcctttcaa gctccttatc tttcataagt tcgtcaacgg agggtatgct
     3421 gctcaatatg ctctttttat caatcatatt tttacctcca aaatcagatt cgaaatacta
     3481 gcgttacctc aatcctcaca gctcgcagct cgtgtctata ttacttctat tgaatattta
     3541 ccctttgtct ttaccttccc tattatgttg aaatctgttt tattgttttt tcgaagttcc
     3601 tccatcagta tctcactgtt ttgctcaggc actgatataa gaagtccgcc cgaagtttga
     3661 gggtcgtaca tgatatccct gacttcctgc tttatgcttt tatcaaatct gatatgctct
     3721 ccaataaatc cttcattatt gtatgcacct gcaggaatta tccccatttt tgcaaaatca
     3781 atgctttcct taataatggg aatataatcc gaccatattt ccagggaaac gttgcttgct
     3841 tcagccattt cgtaaccatg ccccaaaagg ccgaagcctg ttatatcagt acagccgttt
     3901 ataccaacct tcatcattgc ttcacaggca tccttattaa ggtatgacat aacatccact
     3961 gcctttttat atgcctcttg atcagtaata tccgctttta ttgcagtatt caatatacca
     4021 atgcccaagg gctttgtaag tataagcata tccccgggct ttgccccgga attggcggtt
     4081 accttgccgg gatgtaccat tcccataacg gaaaggccga atttcggttc atcatcttct
     4141 acggaatgtc ctccgacaac tattgcgccg gcttctttaa ccttgtccgc tccgccctta
     4201 agtatttctt ttagaatatc aatcggcaga caagtgggaa agcatactat attcattgca
     4261 gtaacaggcc ttccgcccat agcatatatg tcacttaaag agtttgccgc tgcaatctgt
     4321 ccaaatgtat atgggtcatc tacaataggc gtaaaaaaat ccatagtcaa aatcacagct
     4381 ttatcttcat ctatcttata tacggcagca tcgtcggagg tatcaatacc tactatcaag
     4441 ttttcatcgt aaaactttgg cagctggcac agaacctgcg ccagggtctc cggacctatc
     4501 ttggcagctc agccggagct tttggtcatt tgtgtaagcc ttgttttgtt tttgctatcc
     4561 ataatgccgc ctcctttcaa cttcttgcta tatttcaatt tccacattat tataacatat
     4621 attccaagaa tttttgacta aatgttttac ggtatattca ttgcataata gaagaaaact
     4681 gcaacatatc tgaagtttgt ataaccctcc gcaagagaca gtatagtata tcgaggcaca
     4741 cggattatga ttttgacagg cctactgcta tactatacaa gctcatgcct ggttatacaa
     4801 cttacagaat actgccttgt atataaagga aatgagaata ccgacgtggg gaagatatct
     4861 ttgacgaaac tttccatctg accccggaac gggaatatct ctggcaaaat aaccagacgg
     4921 gcacgcagag atgggaccct cgccagtgat tttgctggct ttggaaagtg aggagaacat
     4981 actttcccta cagtcggagt acttcacaaa cttattgtga agcattcttc tattctaccg
     5041 ttatcttatc cttcatgttg ttgaactttg cttcccctat accgcttacc ttcttgatat
     5101 cctcaatggt attgaagagc cctttctgtt ccctgtattc tatgattgct tttgctgtag
     5161 ccggcccgac tcccttaaga gtatccagct ctttttcacc tgctgtatta atatttacct
     5221 ttcccggctt gttaactgcc gcactcttga ccgtattgac ccccggaatt gcctttcctg
     5281 tgtcttgagg gctttcaccc ttctttggga tgtatatcgc atcttcatca gcaagtctgt
     5341 atgcaagatt aacagcctcc aaatcagctt cctcaacagc tccccccacc agcctgatag
     5401 cctcatcaag ccgggttccc tccaggactt ttactactcc gggattcttg acaaaaccgt
     5461 agatatatac ggatatttcc gcgggctttg tttcagcctg tgcttccgcc tgatccggta
     5521 cagcctcagc aaggcttatt tccgtggcct tttgctgtct tatatatata gtaccgctta
     5581 ttatgaccag aacaagtatt acaaatgcaa gtattgattt ttgtgtcctt gtgatataaa
     5641 gcatcttgaa cctttaccct ccttcatctc attgataata tacttatttc tgcattttct
     5701 attaatttcc ttcttttcca tgtaacatta tttatatcat atatttccat ttcaaatatt
     5761 caaaaaaccc tttccaaagg ggtgccatcg gcaagctttt ttgatatact agtgattgtt
     5821 aacacgacag cttttggatt tttcaccgtg ttaacaatca caacacctat tcagtgcctc
     5881 gcatgctcaa gaagtgatat gctttacgag gcacatagtg atcgttggca caagactcat
     5941 tcaatttacg ggataaacat gattcacaag gagaaatgga tgaaaagatt aattgcatat
     6001 attatgatat taagtttgat gatgcctttt gtacgggttt atggagatgt gcctgaggtt
     6061 accgctccgt cagccattct gatagatgcc gagacaggaa agatcctgta cgaaaaagac
     6121 gcggatgaga agcgttatcc ggcaagtacc acaaaagtga tgacaggact tctggcagtt
     6181 gagcacggga agctggacga aatagtcact ataggcaaga atcctcccct tgttgaaatt
     6241 ggaagcagtc agatttacct tgtaccgggg gagcagctta ccatggaaca actgctttat
     6301 gccttgatgc tggaatctgc caatgatgcc gccgtagcca tagcagagca catttcaggc
     6361 tctgtggagg aatttgcaaa acttatgaac agcaaagcca gagaactggg tgccgcaagc
     6421 acaaactttg tcaatcccaa tggcctgcac aatgataatc attatacgac tgcaagagat
     6481 cttgctatga ttgcaaagta cgcaatgact ttggataagt tccgggatgt ggtcaaagaa
     6541 gtcaaatata ccattccaaa gacaaataaa caggaggaaa ggaactatat aacaaactcc
     6601 aacaaactta tatgggaaat atacgatcaa ttcagatatg agtatgccac aggcatcaaa
     6661 accggctata ccaccaaagc aaaacagtgc ctggtaggcg gggcaaagaa aggagacctg
     6721 gagctgatat ccgtagtcat gggtgccgaa gggcaaaaca tgtacagtga cactgtagcc
     6781 ctttttgaat atggtttttc caactttcag aaggtgcaaa tactgaaaaa ggatcagata
     6841 gtaactagtg tgccaataaa agaaggccag gaaaaaataa atctccttgc tgctgaggat
     6901 ttctcaatgg tgctttccga atctgaaaaa gagaatatta cgacagaaat taagaccagg
     6961 gatgggttga gggagcctat cgaaaaaggt gaggtattgg gagaaattat tgtttatgcg
     7021 gaggggaatg aagtaacgaa gatagaactc ctctcctctg tatcagtcga agctgttaag
     7081 aagccaatct tgtggtggct ttgggccatt gcaggttttc tgctgtatcg gacagtagtc
     7141 accatatata aagtaagacg cagaaaaaaa tacagaaacg tgacttatat aaaaagataa
     7201 gggcacgggt gggacatggg gactgggaga catggggacg tttcccctgt ctcactttgt
     7261 gagacacgcg aaacgtcccc gtgtctcccc agcgggacag gagaaacgtc cccgtgtccc
     7321 acacatgttc tttatcctat atatgctact gcttcaattt ctataagcgc atccttcggc
     7381 agccttgcaa cctccacaca ggatcttccc ggatgcttct cagtaaaata ttgtccataa
     7441 atttcattga tttttgaaaa atcattcata tccttaataa atactgtagt cttgagcaca
     7501 ttctccaaac tgctccctga agcttcaagt acagccttga gattctcaag gcactgtttg
     7561 gcctgaacct ggataccccc ctcgggaaat acacctgtag cgggatcaac tcctatcgca
     7621 cctgaagtaa ataccatgtt cccgtacttt acagcctggg aatacgggcc tatagcccct
     7681 ggtgcctttt cagcactgat gatttctttt gtcattatat atcccttctt tcattattaa
     7741 tcatttaccc tcgtctcatc cagataatta taaacagtat atcttgatac gcaaagaacc
     7801 ttggcgacat agtcgatggc tccttttatc aggaatacac ccttttcgtc cagcaactga
     7861 actatattca ccttttcctc tttagatatg aaggcaaccg gcttccctac ggattccaat
     7921 gccttattca ctatactgct cagcatctca tttaccgtgt ttccaaaaca ttcatcttct
     7981 tcgctgaaat ccgtttcttc gatcacattt gccagattat taataatgtt tctggctatt
     8041 gtaagctctg aaatatcata attgatgcac agaaaaccaa taagcttccc attttcatcc
     8101 tttataaaca atgacgagga tttcagaatc ctgccgtccc ttgtcttctt tctatagttt
     8161 actataggct tgtcgaattg tccgctgcgc agtgtagcca aaccatactc agtcataggg
     8221 cttccgatgc ttcttccggt tatgtgtccg ttggctatcg caataacaga gctttgcgga
     8281 tttctgatat catgcagtac aacttcgcag ttttttccaa atgtatttgc tatcccatct
     8341 atcagcggta tgattccttt aagtatggga tgcaggtctt tactcatact aacaccccta
     8401 aacaatatag aattattatc tattcattat ccacaagaac cgtttttgca tctacccaga
     8461 gtctttcaag attatagaat tgcctttctt catcatggaa caagtgaact attacatttc
     8521 cataatcaag taatatccat ctgccgtttc tgaatccttc cttatgatac atttcataac
     8581 ctgcaagttc cattttttct tccaattcat cgctcattgc ctgtacttgt gttgtagagc
     8641 ttcctgcagc aattacaaag taatctgata tagtagtaag ctcatgaata tccaatagcc
     8701 ttatatcttt tgccttcttg cccttcaaga gttcgatggc tttattcgcc atttccgttg
     8761 ataccgaaaa caaatgcacc cctccgttta tctctattat cttacaattg ttatatcata
     8821 tttataacca ttgtcaatac taacttatgt catcttaagc caataccgct ttccgccccc
     8881 tccggagcat tatcattatc tgccggcagc aattccatct cctctgcagg ctcctctact
     8941 ccaagatcta tccgtatctg ctgtatcatc tccttgagct tggcctcatc atgcaaaaag
     9001 tatgagactc tgtttatgta tccgtcgctg cccggaagtg tataaaaact cacattctcc
     9061 atttttatgt ctttaatatt aatagcatag tgtgctatat cactgctttc aagattggtc
     9121 ttaatacatt tggacattgc atcgacgatt gtaagtgctt ttggtatcat cttggggctt
     9181 gtaagcttgt cgataaatgc ctttataagc tgctgctggg ctctaatcct gccaaggtcc
     9241 tggtcgggat atcccgatct gaatctggca aatttcacag catccttacc gttgagcact
     9301 tgtttacccg gttttaaatg aatatacaaa tcctgaacag ggtcctcata atccatacca
     9361 gggggcacat ttaccgatac tcctcccaat atatcaatta tcctttcagc tcctttaaaa
     9421 gttaccttaa cataatgatg tatgggaaca tccagaagat tacccacagt ccgcatggca
     9481 agagcagtac cttctttttt tgcagctgcc gcattaatct tgtcaatgcc gtatcccgga
     9541 atctcaaccc tggtatctct tggtatggaa agcaatgcaa ttttttggtt ttccttatta
     9601 tagctgacca caatcatagt atcagatctt gcttcatcaa tacccattac caaagtgttt
     9661 attattacat cctctttcgt gtcttcgggc tcaagttcgg gattctcttc aatcgtcctg
     9721 acaaagtgct catcagtcat atttgattta tatttattat atactccgcc gattattgca
     9781 aaaaaaacta aaaacgatgc caaaaaaatt attatatatt tcttcaagct tgtaaacctc
     9841 gttttctaaa tattttacgc cagacacagt gatatttcta tctgctgctg tcatgatact
     9901 gcatcccatt ctctccgact tctttaagca catggtttct tgcaagtatt gtattaatat
     9961 gcacatattc gttttttgac aatatgtaca ggatagacga attaaaagcc agaagcagag
    10021 ctttgtccag gttcttgaag gctagtcccc tcagctcttc cactccagga aaactccttg
    10081 agggttcgat aaaatccgaa agatagatta ttttttcaag aagtgacata ttctctttac
    10141 cggttgtatg atatcttaca gcgcttaata tctcctcatc ctcaatggca aaaacttttc
    10201 tgcaaatatg tactgcagca gggccgtgca acagctcctc aatattcaaa gtcagttcat
    10261 ccgcctcaat accttcttcc ttaaggcatt tcagttgaag gggcttttca aggtccctgg
    10321 cacaatcatg caccaaacct gcaatttccg ccttgactgc atcgtattca taaaattcgg
    10381 caagcttctt agctgtttta ctcacattaa cagaatgtat atatctcttt gaggatatgg
    10441 tcttttcaag atgctccatt attgaattca aatccagcaa ataattcact ccttgtaaag
    10501 tccgttttta tgtatatatg cttccacctc ttccgggagc agatatttta caggctttcc
    10561 gctccttatc ctttctctga tatctgtaga ggatatttca agtaatggaa cttccaggca
    10621 tataatttct ccatcatatt tagacgtaat ttccgctatc ttttgttcca gcttacaaat
    10681 atcaaatccg ggcctggtaa cagcaataaa tttgcagagc gtcaaaagct cccctaactt
    10741 gtaccacgtc agcagctcca gcactgcatc ggcccccgta ataaaatata ttgcgggagc
    10801 ttcgccatat atgtttctca gttccttcat tgtatcaaaa gtatatgtaa tcccggcctt
    10861 atccacttcc aggcgggaca cttcaaaccc ggagttagaa gaagttgcga gagctgtcat
    10921 acgatatctg tgctctgcat cgcttatcgc ttgcgactgc ttatggggag ggtttcccac
    10981 aggcatgaat atgaccttgc agagcccaaa ttctattctc gctgcctcgg ctgccgcaag
    11041 gtgtccgtaa tgaatcgggt caaaggtgcc tcccataatc ccaatcctgc tttgcccatt
    11101 gtcaactgca tcctcgtaca taaaaatacc tctgtacttt tttatgaatt acgtccatta
    11161 tacaataata tacagagttt ttaaagtttt tccactaatt tccgatagat atcttcaata
    11221 atatccgcag ctttactgtc attacaaact gtgaaatcac tgtactcctt gtacaaatca
    11281 tacctttcgg catgcagctg cagcagccgg cctgtccccc ctgccagcag cggcctcgtg
    11341 cttgtatcaa tgtcatccat aatatcacat acatccctgt caataaatat aataattccg
    11401 ttttttttca ggcttttcat atttacagct ctttttacca caccgcctcc ggttgaaatg
    11461 actaaagcct tttccctctc cagagcaagc accgccttag tttccagatt acgaaagtgc
    11521 tcttccccct gccgaaacag ctcgttaata gtacatccct cttgtttttc aatcatatca
    11581 tctatatcta tgaacttcct ctcaagcttt tctgcaagca gccttcctat agacgtcttg
    11641 ccgcagcccg atagtcctat caatactatg ttcttgcttg tgtctgcctt atagagcatc
    11701 ttttttatat gctggtaaat attatccacc tgtgaccttg agagtccgat tccattccag
    11761 atttcctgag ctgcaacagc ctggcccaca agcatgtaaa gtccgtttac cgctttcagc
    11821 ccgttttctt cagcctgctt taaaaacatt gtcttttccg gattatatat taaatctact
    11881 gctgtaccaa actgcttaaa tgttccataa ggaaccggtg atacatccgc cttggggtac
    11941 attccgcagg gagtgcaatt tattataata tcccccttca ggctttctgt ttcatcatag
    12001 ccgacagccc catcaccggg atttctgctc acatatgtga tgcgctttac tccattatcc
    12061 atgagatact gcgctgctgc ctttgaaact ccaccggtac ccagtattac cgcagtttta
    12121 ttttttacct ctatattgct tcttgcaagc agcatgccga atcccctgta atcggtgtta
    12181 taccctgtga gtacatcatt ggccagtgaa attgtattta ccgcaccgat tttttcagct
    12241 tcttcagaaa tcacatcaag gtaaggcata atctttattt tatatggtac agtgacattc
    12301 aatcctttga tgccctgctc cctgtacttc aggagcgcag gctccaattc ctcttcctga
    12361 agttcaaaaa gcctgtattc tccttctatt cccagcattt tgaaaatttc acaatgtatt
    12421 tgcggagaaa agctgtgtct taggctttct ccgattaatc cgaaaacatt attcatcatc
    12481 ctcacccaac ctcgcttcaa taattaccca gcaatctgaa atatgaacat tccttctcca
    12541 tatccgccag aatgcctctt ataccttcct catttatgtt tccgctgaaa tccgtataaa
    12601 agaaatattc ccatgcctta tcttccaaag gcctggactc aattttcatc agattgcaat
    12661 taccctccgc aaaatacctt aatattccat aaaggcttcc cggcttatga ggcacactga
    12721 atactatgct gattttatca gccttttcat tatcaagctt ttctttgcta ataattacaa
    12781 atctggtact gttgttcctg ttatcctgta tgccccggca aattatacac aggccatata
    12841 ctgaagctgc cttctcacct gcaatacatg ctttggattt caagccttcc ttcgcaatgt
    12901 actctgcgct tcctgcggta tcaaaatagg gtatctgctt ccattcgggg tattgtgaaa
    12961 gataacggct gcactgaatc aaaccctgcc ggtgactgta aatctcctta atatcagata
    13021 gctttgctcc ctccaccgca agtaagctgt gctccactct caggcatttt tcgcctacga
    13081 tatagaattc atattctcta agtaaatcgt atacttcatt aatactgccg gaggtggtat
    13141 tctctatcgg cagtatgccg tactgtattt ccccttttgc aagagcatcg aaaacatccc
    13201 tgaagctgtg atagctcaag gcctcaatcc cttccccgaa atattcaagc cccgcctgat
    13261 agctatagca gcccggcacc ccttgatagc ctattgccat gttcgtattg aggttttcta
    13321 tcttagaagg ttcctgcttt ctgcctatta tatctccctg aagcctcctg ctcaaaaaca
    13381 tcaggttttc caaaaactgc tcaatatact ttatatcaaa atcgactctg actctattga
    13441 caaacctgcc aacaatgtct ttttcccttg ccggatcata tatcctaatg tttttccctg
    13501 atttgtactc cccgaccttt ttggcaagtg ccattctttc tataaacagc ttcacaagct
    13561 tttcatcagt ttcatttatc ttctgtctta agatttcaag ttcatccaat gcaaacacac
    13621 cctctattca ttcattttcc ctgactaacc ataataaggt ctaaaagtgc catagcagta
    13681 actgcctcta ctacaggcac tgctctcggc acaatgcacg gatcatgtct tccctgtact
    13741 tccaatccga cgttttcttt cttctcaata tctatggccc gctgccgcct gccgattgag
    13801 gcagcaggct ttatggcggt tcgaaatatt atcggcattc cgtcggttat gcctccaagt
    13861 attcccccat tgttgttgct gtaggttttt acttcctctc catccatata atactcatca
    13921 tttgcctcgg aacccataag ctttgatata tcaaaacccg ctccgaattc caggcctttt
    13981 actcccggaa tggaaaatat gagatgagct aaagtgcttt ctactgagtc aaaaaaaggt
    14041 tctccgattc ccggcttcac gcccagtact gcagtttcca caacaccgcc tattgagttc
    14101 ttttgctcct ttgcccgggc gataacctct ttcataaggg cttcgctttt tgcatccaat
    14161 acgggaaaag ccttgcccgc caattttttt aatgtgcctg agtcaacgct gacaggattg
    14221 aaaggcctgt cctgctcttc tcctatgctc cttatgtgac tgcctatggt tattcccctg
    14281 ctttcaagaa gctgtttgca tatcgctccc gcaaaaacca gcggggcagt cagcctcccg
    14341 gaaaaatgcc ctccgcctct atagtcattg aagccgccat gcttcacata acctgtgtag
    14401 tcactgtgag aaggcctcat tatatttttc agctcttcat aatcttcgga ttttttatct
    14461 gtattatgta tcactgcaca taaaggtgtt cctgtagtcc tgccctcaaa aaagccgctt
    14521 aaaatttcga attcgtctcc ttcctttcgt ggcgtggcaa actcactatt ccccggaacc
    14581 cgcctttgca tttcccttct tataaagtca agatccagaa ggattccggc tttcaagccg
    14641 tcgatattta ttcctattgc tttaccgtgg gattccccaa atattgatat ttttaagctg
    14701 ttaccccata taccgctcat cgattttccc ccccaatgct ttataatgta cccagaaatc
    14761 aggataagac tttttcacac aagaagcatt gcttatgata accggctctt cgcaccttgt
    14821 ggctgctata gccagtgcca ttgtaatcct atggtcattc cagctttcaa cccttccgcc
    14881 ccgcaggttt tttctgccgt caataaccag gctgtcatgc aattccataa tacacgctcc
    14941 cagtttgcca agctcgctgc ttacagcctt cagcctgtcg gattccttca aacgcagccg
    15001 gcccgcattt actatttcag tccttccctt actcagtgcc cccagaactg ccaatatcgg
    15061 gaccaaatcc ggacactggg aggcgtcgat aactgttcct cttgtatcag ccggaagcgc
    15121 cctgacagca gcagctttta taaatattcc gcctcccatt tcgccaatta tgtccatcat
    15181 tactctgtca ccctgcatgg aatttatatc aaggcccttg cataccacct ccgaccctaa
    15241 tgctcccgct gccagccaga aggcagcctg tgaaaaatct ccgggaacgg tataatccgt
    15301 cgctttgtac ttttgccttc cttttattat aaaagtcctg taaccatggt tttctatatg
    15361 tattgaaaag tccttcagaa ccttcagtgt aagatctatg taaggctttg actccggctc
    15421 tgtggttacc gttatcagtg aatccccctc caaaagcggc agtgcaaaca tcaagccgct
    15481 aatgaactga gagctgatat tcccctccag ttgaaactct ccgggaacca gcctgccgtt
    15541 aatgtgaaga ggaagctttc ctgcctcggt ttcgtatttt atcccctgct tctcaaaaat
    15601 attgtaatat tgcagcaaag gcctttcaac cagtctccct tctccgtaaa aagtaacggg
    15661 ttcacccgtc agggctgcaa tgggtataat aaaccttaag gcagaacctg attcatgaca
    15721 atttattccg gagtttttca gcttaggtac ggcactcccc tttatcctca gtgaggcatc
    15781 cgccttttca atcattgtac cgaagcttct caatgcttct atagtcgctt caatgtcctg
    15841 cgatattccg acattatata tgttacttat tccttccgag agtcctgcgc atatcaccgc
    15901 cctatggctt atgctttttg aggctggtat gaaaacctct ccggcaaggc tgccgggata
    15961 tattttcact tctttcatcc ttaatcctcc cttgcatctc ccgttgtatc aagaaagctt
    16021 tcaagttctt cggattttgc tttcattata aaggcagttc ccagtttatt taataataca
    16081 agccttatac tatcaccgct gaatttttta tcccgcctca taacatttat cagatctttt
    16141 ttatttgcaa cagggatttc ataaggcaag ccatatttct ttaatatatc cgccaaagct
    16201 tttgcagtac cccgttctgt taatcccatc tcctcgctcc gttttgtaat ttccgccata
    16261 cccattgcaa cagcttcccc gtgggtatac ttctcataat tataatattg ttctactgca
    16321 tggccaaagg tatgaccgaa attaagaagc attctttccc ctctgtccaa ctcatccgct
    16381 tccactactg ctctttttat cgaacagcag gtgcatacca attcatccat atcggcaata
    16441 cattgctgca ggttttcata atccgaaagc ctgtcaaaga ggctcttgtc ctttatgcat
    16501 ccgtatttga ttacttcagc catgccgtcc ctcaaatacc tgtcttccag tgtattcaga
    16561 aataaagtat caataaatac aatttcagga tgataaaagc ttcctgccag gtttttcccc
    16621 gaggaaaggt caattgcagt tttaccgcct acgctgctgt ctacttgcgc cagtaatgtt
    16681 gtcgggagtt gaatatacga tatgcccctt aaataggtag ctgcggcaaa ccctgccaaa
    16741 tcgcctgcaa ccccgccgcc aaacgcaata atgatatctc ccctgtctgc gttgaaaccg
    16801 gcaagatcct catatatccc cgaaaggact tgcagggatt tgcttttttc acccggacta
    16861 aaggatataa gcctgcattt catccctgcc tcaactatac ccctatataa tgatgctccg
    16921 taaagcttat ttacattatc atcggtaact attacagcct ttttgcccct atatctctct
    16981 gaaagatatt tataaagctc ctgccgaatg tttttgccga tttggatgtt atattttttg
    17041 ctgctgcttt ttattgaaac ctctatagtc accatctata taatctccct tttactttta
    17101 atagcattgt cgaacagtat ttgaaggctg ctttcgtcct gttcttttat agcatctttt
    17161 aatttatgaa gcatgtcctc catatcttct attttatcaa taatattcaa attattcatc
    17221 ctgagaattt gagtccagag ttccttattc aatgccgcca ctctggtggc atccttgaag
    17281 ctgcgcccgg taaaagggct tatgcctttc tccctcatca tcaggtttgc aaacgagata
    17341 gcaactatat gcgggagctg gcttgtaaag gatatcaaac ggtcatgagc ctctgctcct
    17401 atcctggtca aacacttgca tcctattgaa gctgccagct tttccacagc tattatcccg
    17461 ttttcgctat tgctttcatg gggtgtgata atatagttag tatcaaaaaa gagttcccgg
    17521 gatgcggctt caaaaccttg aaactcattt cccgccatcg gatgtccccc gacaaactcc
    17581 acatcctgcc gcaagctttt ctttgcagct tcaattatcg gctgcttgat actgcaagta
    17641 tcggtaacaa tggtccccgg tttgaaatat tcggcataat tcttaataaa tccaattgct
    17701 tcccgcggat acaaagcaat tattaccata tcggatttgc ataatatttt ttttgcattg
    17761 gaagcaccct cgtctattac tccgcagccg gctgctttgt tcaacacgct ctcatcaata
    17821 tctatgccga atattcttac tgcattatgc tttctcagtg ccatggcaaa agaaccgcct
    17881 attaatccca agccaactac tgcaatattc cttctaaagt caccctcttc caatttaatt
    17941 cctccaatca tcgcaacact taactgatac cattcccagt attttcttta tgttccgccc
    18001 tttaattagc ctttttcagc gagtccgcca ttaatgtaat atctttcatc attgccctga
    18061 acttttccgg cttaatggat tgctgcccgt cacacttggc atttgcagga tcgctgtgca
    18121 cttctaccat aattccgtca gcacccgcaa caaccgatgc ttttgccaga ggttcaacca
    18181 tccaccacaa gcccgtcgca tggcttggat caactattac gggcaaatgg ctgagtttct
    18241 tgattgccag aatagcgctg atatccaaag tatttcttgt ataggtctcg aaggttctta
    18301 tgcctctttc acaaagtatt acattgctgt tcccctcaga catgatatat tctgctgaca
    18361 ttagcagctc ttctattgtt gcagacagtc cgcgcttcaa aagtatgggc tttgtggtct
    18421 ttccaatctc cttaagcagc tcaaagttct gcatgtttct tgcgccaacc tgtattacgt
    18481 ccacatcttc tacgaatctc tctatcatat ccactgacat tatttccgtg acaatcggaa
    18541 ggccggtttc cttccgggca agtttcaaaa gctcaagtcc ctcaagcttc atgccctgga
    18601 agctgtatgg cgatgtcctg ggcttgaatg cgcctcctct taagaagcct gccccgtact
    18661 ctttcacctc tttcgccaat tccaatatct gctcttcgct ttccaccgag caagggcctg
    18721 ctattacagc cagttcctga ccgcctatgg tcttgtcgcc tacctgtatt accgagttgt
    18781 ccttgtgaaa cattctgttt gaaagcttgt atctctgttg aatcttcatg accgcttcca
    18841 cgctttcatt tgcttcgatt tcacttatgt ctataccggt agtatctccg ataattccaa
    18901 gcacacaata atccctgccc tttattacac ttaccttgat atcttctgtt tctatccttt
    18961 ctctaagttt ttcgatttct tcatctgagg cattttctct cattactatc accataatta
    19021 ccctccttta tattttctaa aaaatgtgtc tcacggcgcg tttttttcgc tagggaaccc
    19081 tatggttccc ttcgacggat gcaagcatcc tgagcaccct cctttatcgg cagggagctt
    19141 ctcccccttg catcccccat cgtataggaa attattattt cctatacgat aaaaaaaata
    19201 aggctctaat gagccttatt tgaatcgatg ctatttcata cgaataaaag ttttacttac
    19261 aataattttt aaattcttcc aactcatcag gaaaaggaat atattctttt ttgcatgcat
    19321 aataaccggc gctaaagtaa tagcccaggt taaagctaaa gtaatatatt ccgcttattc
    19381 ctttccctaa taaattattc attgcataaa tctccatact catatgattt gaatcaaatg
    19441 tatttctgtt gattataaca tatgcatcaa atatgtcaac tgttatttct tattcagagt
    19501 atgcacaaac acgattcccg cgaaacataa atc
//
LOCUS       DAOOAB010000051        19519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3720_length_19519_cov_16.747020, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000051 DAOOAB010000000
VERSION     DAOOAB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19519
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3720_length_19519_cov_16.747020"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1309
                     /locus_tag="PKU88_10110"
     CDS             167..1309
                     /locus_tag="PKU88_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPX69667.1"
                     /translation="MIYLDNAATTFPKPESVYRAVDDCLRNYCANPGRGGHKLSLQSG
                     RVLLEARELLAELFNADSPDNVILTHNATEALNLALKGFLKKGSHVITTSMEHNSVMR
                     PLKVLEKLGIETTIAQCNEYGEIDIGSIEKEIKKETALIAAAHASNVVGTIIPIEDIG
                     DLAKRYGLAFLVDASQTAGVYEIDMQKMNISMLAFAGHKSLMGPQGTGGLCIKRGLEL
                     TPLQEGGTGSKSESLYQPDIMPDRFESGTHNTPGIAGLCAGLSFIKSKGIDKIRAHEQ
                     ELVKYFLDGLAEIKNVKVYGVKEASKQAPVVSINIGDTGSSEISYILDRTFDIATRSG
                     LHCAPMAHKTLGTLEQGTVRFSFGFFNTKEQVGAALNAVENIGTLL"
     gene            1321..1827
                     /locus_tag="PKU88_10115"
     CDS             1321..1827
                     /locus_tag="PKU88_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HPX69668.1"
                     /translation="MNIMDYFIKVYNAEILFSITILLFIMIVWNLILSVRLAGMSEKY
                     KKFMRGSSGKNFEGMLLGHIENMEASIERVNIISEDLIHVKNQMDKCIQKCNIIRYNA
                     FSDTGSDQSFSIAMLDNFNDGIIITGIYGRNEFVGYAKPVSKGTSRYPLSVEEELVLN
                     RSLKGTQI"
     gene            1894..2172
                     /locus_tag="PKU88_10120"
     CDS             1894..2172
                     /locus_tag="PKU88_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HPX69669.1"
                     /translation="MIIAIQHGLEDIKKELESRGNEVFYIGENRMADAVLYKEADTRP
                     YYAVNNVPSTAALSNENISYGALLVNVTNKSMDDIIRILEKRIYSPLL"
     gene            2281..3450
                     /locus_tag="PKU88_10125"
     CDS             2281..3450
                     /locus_tag="PKU88_10125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPX69670.1"
                     /translation="MYQGVYDRILLIAMTCFSFFIALYTISMNKTVSLFKLLGWVFFI
                     PFGLCCSIFVFSPDTPINLILSFSEFCLAAGILLLILYPYNTKNQKLMIVVIAALTLI
                     LSVYAIKTEHFIYYTFSAALRNILTAACIIAAIVFNIKKNADRKLAKPLAIWLTGMLL
                     GFVENKAYINEAVFAVKLSAYACFLFYFFRTTHDLLMKKVNESEELIEDMKKSLNKEV
                     KKRVFEIERTNEMLLEISKTDMLTKTFNKITILNIIEKLITSKKMEAFSILMFDIDNF
                     KTINDSLGHVTGDMCLKTLSAIASGNIREVDYIGRYGGDEFIIVLPTLSVNEARFVAE
                     RFRKKVSETSNPRFTVSIGIATYPHDGQTVHELIQAADRGLYKAKSMGRNTVCRA"
     gene            complement(3455..4102)
                     /gene="yyaC"
                     /locus_tag="PKU88_10130"
     CDS             complement(3455..4102)
                     /gene="yyaC"
                     /locus_tag="PKU88_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HPX69671.1"
                     /translation="MLKIKYERTVDINKPYSFSYFCNYLEECMSIFYDKNHTDIVIVC
                     IGTDRATGDCLGPLIGYKVKDMRYKNVHVYGTLDEPVHAKNLCEYMELFRKFDNPFII
                     AIDACLGRYERIGFINIKEGPLSPGSGVNKNLPAIGHINITGIVNVGGFMEIMILQNT
                     RLSVVMNMASLIANGLKYNMWKMNRRALSDNSLINRQIASTASEDKFFDYMENKN"
     gene            4363..5460
                     /locus_tag="PKU88_10135"
     CDS             4363..5460
                     /locus_tag="PKU88_10135"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HPX69672.1"
                     /translation="MQKVRSKKTTALLLLLIIVSVLAILLYINNKTTRKSSFIAGGKS
                     KLAVTGEVTAVSQSSSAIYYVKDLLIEAQAENIRAKNINGDIAWSCKLPGKAVKMVDA
                     GQNIIIIDSANNIHYYTLQGKLLWTYKSDYEIVDIFTEDNGSFLAEYKGMTGSHAEAF
                     KQDGSKIGSISVDNAHILSFAGGSGVFSISIVDTSSEMIKTKIITYSFKGDILWAQNF
                     DNVIISKLSFSKDNRLLALGENTVYIYKNDGSLHQEVKIEGEISRAAMNGHIVALALE
                     IEDKQYLVCYDANMREQSRTEIKPEPLGIFPLKSNLIVYYSDELMILTLKGELNARYE
                     PNVDISAAYMTSDNKVYIASNRRLLQLEYIK"
     gene            5482..6111
                     /locus_tag="PKU88_10140"
     CDS             5482..6111
                     /locus_tag="PKU88_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPX69673.1"
                     /translation="MNWIDYLIIAILAVGVIIGFRKGFLKSVFSIICLLVSIMVAKTY
                     YKALTMFLVENTALEEKITGFLSEKAFVKNILMSPSGESAVFSISNSFTSDLNAFVTV
                     LIINAISVLIIFIAVRFILGIAESILLDAVELPGLREVNSIGGAIVGLAKNIIIIMLI
                     FTIITPISAIKALSGISDGIEASALAEYFYAYNFILGWIWTAAVDLLNK"
     gene            6138..6770
                     /locus_tag="PKU88_10145"
     CDS             6138..6770
                     /locus_tag="PKU88_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69674.1"
                     /translation="MNKKNALIGIALIFLGISMYLKNYNIGTGSLLTLFLGLGLLYAY
                     HIKREQPFIIFGGIFAAIGLAAVLRDIRLFRIDMVFEAMLIALGIIFIFLYYSKHIEG
                     FIFPGVILPAAGVYLLLLRSFNDRYVSPSIFLLLGFAFYVIYLIAYMGKSNWPLIPAN
                     ILLMAGIFAYAVSFEIITWHMVYLKRDYIWPLLMIGAGVLILISRFRRRA"
     gene            complement(6889..8139)
                     /locus_tag="PKU88_10150"
     CDS             complement(6889..8139)
                     /locus_tag="PKU88_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPX69675.1"
                     /translation="MEEKMLILKMLQEGKITTDEAVKLLEALEKGGSGTPSSGSKINE
                     IKDELAAKLNEIKIDEKLNKFGEKASKLASTFGEKAGKLVEQLGENINTDKTGNNAEK
                     FAEEFTKRMEILGQDIAESAAKFADVFTSQIDNLFDTVYEKHKYNSSYTYPVEDTLSL
                     YLETGNFSVKACPVDTKEIKINLYVNSNVPQLAIDEYFKVIKDTEGYRLSTEFPGRTW
                     GRIEILLPKGMDVISFSTNNAKCEISDIDANHISCATANGRVILTRCSADEIEIFTDN
                     ERIIFNEVSARTANIRTSNSKIAVEDSRLDNIDAKTSNAAIAVSTSRKGDSLNPCYTL
                     STSNGKIDFSLAGEEGFEYMVDAHTAMSSIDVKLANLTYETEKKNIGMQGSVQLKSDN
                     FDTASNKVSITANTTNAPISIRNI"
     gene            complement(8151..8513)
                     /locus_tag="PKU88_10155"
     CDS             complement(8151..8513)
                     /locus_tag="PKU88_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HPX69676.1"
                     /translation="MKREVMGQCPICSGPVEITEVSCNSCKSTIKGHFKPCKFCSLSQ
                     EHKEFAEIFIKNRGNIKEIERELGISYPTVKGKLDSLISALGYKSQSVDQSYKKEILE
                     RLYRGEISSEEAIRLMSE"
     gene            8947..9525
                     /gene="yedF"
                     /locus_tag="PKU88_10160"
     CDS             8947..9525
                     /gene="yedF"
                     /locus_tag="PKU88_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPX69677.1"
                     /translation="MKEIDARGLVCPQPVILTKKALEGMTEGEVVTIVDNITAKENVS
                     RLAENLSCKYEISDESGCYYIKIIKAAGAESTETKEDSTVIVITSDKLGQGAEELGKV
                     LIKSYTYALTEATPLPKAVMFLNSGVKLTAEGSEVAENIKKLENSGVEIISCGTCLDF
                     YQLKDKLQVGIVGNMYSIIEKMNSAGKVINIG"
     gene            9537..9755
                     /locus_tag="PKU88_10165"
     CDS             9537..9755
                     /locus_tag="PKU88_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HPX69678.1"
                     /translation="MYYPKQFNIGDIVETKKQHPCGDNHWEVIRVGADFKIKCLKCGR
                     IVMLPRPKFEKSVKKVIKSAAPEENNID"
     gene            9852..10136
                     /gene="rpsF"
                     /locus_tag="PKU88_10170"
     CDS             9852..10136
                     /gene="rpsF"
                     /locus_tag="PKU88_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPX69679.1"
                     /translation="MNRYETIYIIRPTVDEEGIKALQEKFKAIVEENGEIEAIDDWGK
                     RKLAYLIDDIGEGYYVYMKYKAAQVVPKELDRVFGITEEILRHMIIKIEE"
     gene            10178..10588
                     /locus_tag="PKU88_10175"
     CDS             10178..10588
                     /locus_tag="PKU88_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPX69680.1"
                     /translation="MLNKVALVGRLTRDPEIRYTADNQTPIAKFTIAVDRTYKREGQP
                     TADFIPIIVFGKSADNCGKFIKKGRLVAVSGRLQTRNWDDQDGKRHYATEVIADEVDF
                     LDRGSDARGQGNEGRSDSDYGDFHLAEEEDELPF"
     gene            10672..10887
                     /gene="rpsR"
                     /locus_tag="PKU88_10180"
     CDS             10672..10887
                     /gene="rpsR"
                     /locus_tag="PKU88_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPX69681.1"
                     /translation="MRKPKKKVCNFCIDKVEHIDYKDVNKLRRFISERGKIIPRRISG
                     NCAKHQRQLTIAIKRARNIAFLPFTTD"
     gene            11236..11556
                     /locus_tag="PKU88_10185"
     CDS             11236..11556
                     /locus_tag="PKU88_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HPX69682.1"
                     /translation="MLNQDTDITKNIKMIEFLKSELLTTVASLYQNLLKGTKVGQGVI
                     QDILANLVLVTYLLGKRLGVPYSMIDTKILEKIRLGMLEEHETEKWYGDLTDLGDYLK
                     TKKG"
     gene            11566..12549
                     /locus_tag="PKU88_10190"
     CDS             11566..12549
                     /locus_tag="PKU88_10190"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HPX69683.1"
                     /translation="MERMQTRALVEGAIFAGVTALLGILYYYTQYLGIIAMVWPVPVI
                     IVGYRNGIKASILSALSAGLVVSLVTQPLVGVGLLIGFGVPGVLMGHMINKRVNPYAV
                     ILVCGVVLSVSMAGEFLLSLKVAGIDGLEFLSNLDSAFREQLETSLNIYRQFGAAEEK
                     LQEFKDYFSQAIELMKYVIPSSLLISGVFFSFIDYKLTRLILKRIGLILPDIDEFSKW
                     RLTEPYSYILLGTAVLAGVASYFKVPGFNAVVLNISTILSLIFSIVGISAVVYFARVY
                     GDRYGIPKALRRIITVFVILTFIQFIAFIGILDMAFDFRRLKSKNRIGGVR"
     gene            12546..14543
                     /locus_tag="PKU88_10195"
     CDS             12546..14543
                     /locus_tag="PKU88_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPX69684.1"
                     /translation="MKIRKFSDIWGPDVSISLWILAIAVVVIGYYNVFLGLAGALLLG
                     YLVYHNLHAATRKKEELRLYIENLSENVDTATKNAILNLPFPLVIIDDQGVINWYNML
                     FSSIFQGEYILDKKLSSYLSGVDISRILKNKMERFKNINIKDRYYDIYCNLININESG
                     EEKNIIIMYFVDKTDFISLRNRYNDEKPIAAILQVDNIEEVIKETEEALRPALVAEVD
                     RRVNAWAALNNASIQKYSNGEYMIYFSHKQLMSMEEKRFDILDSIREINSGNKIPITL
                     SIGVGVNGKNHAELQKFCTSAMDIALGRGGDQAVVKDNEKLSFYGGKTKAVEKRTRVK
                     ARVIAYALRQLIEQSEDVLIMGHEMPDLDCMGSALGIYRCCKGLNKEVKIVLGGVNAG
                     IANLWSELQEDEEYADVFVSPQDALQICGANTLLVVVDVHRPAFTVQKQLVEKAEKIA
                     IIDHHRRGSEFIENAVITYIEPYASSTSELVTELLQYMFEKPKIKQIEAEALLAGIFI
                     DTKNFSFQTGVRTFEAASYLRRMGADTTLTKQLFQDDMATFLTRAEVVKGAQMFKSNI
                     AISTIPVNAKNPALVAAQAADELLNIKGVNASFVLSCIEGDIFISGRSLGDINVQVVL
                     EKLGGGGHLTVAGAQLPGLTMEKAMSLLKKAIEEYLKEGER"
     gene            14544..14999
                     /gene="rplI"
                     /locus_tag="PKU88_10200"
     CDS             14544..14999
                     /gene="rplI"
                     /locus_tag="PKU88_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPX69685.1"
                     /translation="MKVVLLQDVKNLGKKEQLVNVSDGYARNYLFPRKLAAEATSGKL
                     KELEEKKNSEIIKKDKEKQAAKELADKLEKLEVNFKVKAGENGKLFGSITSKDVADAL
                     KSQHKTEIDKKKIVLHDAIKALGTYKVEIKVYPGISTLINVKVEEQRGV"
     gene            15003..16835
                     /gene="lonC"
                     /locus_tag="PKU88_10205"
     CDS             15003..16835
                     /gene="lonC"
                     /locus_tag="PKU88_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HPX69686.1"
                     /translation="MSHNHSSIRSKDVSIDVLVDFTKKLYSSDKTRKLDNVTDNKEKI
                     YILHEIVSGKIENKRIEENKLEFLIEDIKDKLSDAAAKRYIEEGIKREVIKRMETREI
                     EYLNEMKLQVIKKHAGLENASTLKKYADLELMKKVRLTKSIMDVMRPQRQDEIIGQEN
                     AVSALISKIASPFPQHVILYGPPGVGKTTTARLALEIAKSRKHTPFSKTAKFVEVDAT
                     TLRWDPKESINPLLGSVHDPIFQGANKELAEMGIPEPKPGLVTEAHGGVLFIDEIGEL
                     DVMLQNKLLKVLEDKKVRFDSSYYDVNNENIPRYIKLLFEEGAPADFILIGATTRQPE
                     EINPALRSRCAEVFFNPLSSSDIARIVLNAADKLGARISAKGAELISSYTYEARKAVN
                     ILADCYSSMINEREDKDIEISVDCIKEVLSKGRYVPLNKKKAEGIREIGRINGLGVNG
                     FIGNVIELEAVCFAREDEAGTIRFNETAGSMTKDSLFNALSVIRKITDKNINKYDIHV
                     NCVGGGKVDGPSAGAAITCLLYSAILERPARQDTCITGEISIRGRIKAVGGIVEKISG
                     AKRAGFKNVIIPEENKEEANGLDGINIIPAGNIQEVIGFMFDEQ"
     gene            16854..18194
                     /gene="dnaB"
                     /locus_tag="PKU88_10210"
     CDS             16854..18194
                     /gene="dnaB"
                     /locus_tag="PKU88_10210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPX69687.1"
                     /translation="MSEMIQRIPPNNIEAEQSVLGAMLLDKEAISTATEYISGDDFYR
                     EAHKEIFEAIVDIYNRGEPVDLITLTEKLKTRNTLEAVGGITFLTNLMDAVPTTHNVK
                     YYAKIVEDKSLLRKLIKSSNEIIQKSYQAEEDIGEIIDDAEKGIFNISLKRSTQGFVH
                     LKNILNANFDRIEQLYLNKGKITGVPTGFHDLDNKLSGLQKSDLILIAARPSMGKSSF
                     MMNIVQHAAVREKTTTAIFSLEMSKEQLTQRLLCSEALIDAHRLRIGDINEDEWVKLA
                     RAMGPLSEAPIYIDDTPSISITEMRAKCRRLKLEHDLGLIVIDYLQLMQGKGYAESRQ
                     QEISEISRSLKALAREINVPVVALSQLSRAPEMRADHRPVLSDLRESGAIEQDADVVM
                     FLYRDEYYHPDTEKKNIGEINISKQRNGPTGTIELIWLGQFTKFVNKEKHITAI"
     gene            18707..>19519
                     /locus_tag="PKU88_10215"
     CDS             18707..>19519
                     /locus_tag="PKU88_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HPX69688.1"
                     /translation="MIDIIVNEKDLSFNRLEKEIYAYGCQIAREILKSVLEAMDKRLA
                     EERDKSKYRHRGGRNTSIKTLMGEVEFSRVIYEGKDDEDRKSFIYLLDELLGFDTIGQ
                     ISTNLAEKIVENASITSYRNAADNITELTGQGISHGGVWNVVQALGQKVREEETELAK
                     AAEKNQLCGGKEVPVLFEEADGVYINIQGKDRPKSGKKLEMKVAVAYEGWKEVSKNRF
                     ELVNKVACAGFEDISEFYKIKEAMISREYNVDEIRMRILNGDGAAWIKHGIDD"
BASE COUNT         6286 a         3392 c         4438 g         5403 t
ORIGIN      
        1 gattttacaa gtatcaagct ctgattctgt ccaaagctaa tatcgtgcga gtcggtatac
       61 aaggttattg ctttgggagt atgactgcga attaataatt gcgatgtatc attcttctat
      121 aacgatatat attaataatg tttcacgtga aacaaaggag caaactatga tatatttaga
      181 taatgcagca actacgtttc caaagcccga atcggtatac agggctgtag atgattgtct
      241 caggaattat tgtgcaaacc cgggaagagg cggacacaag ctttcccttc aatcgggcag
      301 ggtacttctt gaagccaggg agttactggc agagttgttt aacgcagatt cacccgataa
      361 tgttatattg actcataatg ccacagaggc attgaatctg gctctcaaag gcttcttaaa
      421 aaagggcagt catgtaataa ccacaagtat ggagcataat tcggtaatga ggcctttgaa
      481 ggttcttgaa aaactcggta tagaaacgac aatagcacaa tgcaatgaat atggggaaat
      541 tgatattggc agcattgaga aggaaataaa gaaggaaacg gcacttattg ctgctgccca
      601 tgcctcgaat gttgtgggaa ctattatacc cattgaggat atcggagatc tggcgaaaag
      661 gtatggattg gcttttcttg ttgatgcttc acagactgcc ggtgtatatg aaatagatat
      721 gcaaaagatg aatataagca tgctggcttt tgcggggcat aaaagcctta tgggacccca
      781 ggggacaggc ggattgtgta taaaaagagg cttggagctg acccctttgc aggaaggagg
      841 aaccggaagc aaatccgaat cattatatca gcctgatatt atgcctgacc gctttgaaag
      901 cggaactcac aacacaccgg gtattgcagg cttgtgtgcg ggcttaagct tcataaagag
      961 taaggggatt gataaaataa gagcccatga acaggagctt gtaaaatact ttttggatgg
     1021 actggcggag ataaagaacg ttaaggtcta cggtgttaaa gaggcaagta agcaggcccc
     1081 tgtagtatca atcaacatag gagacacggg ttcttcagaa ataagctata ttcttgaccg
     1141 gacattcgat atagcaacaa gatcgggact tcactgcgcg cccatggccc ataaaacatt
     1201 agggaccctg gaacagggta ccgtaaggtt cagcttcggt tttttcaata caaaagaaca
     1261 agtaggtgcg gcactgaatg ctgttgaaaa cattggaacc ttgttatagg agagtagggg
     1321 atgaatatca tggattattt tattaaagta tacaatgctg aaattctgtt ttctattaca
     1381 atattattgt ttattatgat tgtctggaat cttatactat ctgtcaggct tgccgggatg
     1441 agtgaaaaat acaagaaatt catgaggggt tcttcaggta agaactttga aggaatgctg
     1501 ctggggcata tagaaaatat ggaagcgtca atagaaagag tgaacattat aagcgaagac
     1561 ctaatacatg taaagaacca gatggataaa tgcatacaga agtgcaacat aataagatac
     1621 aacgccttta gtgatacggg aagcgatcag agtttttcca tagctatgct tgacaatttt
     1681 aatgacggta tcataattac aggtatctac ggaagaaatg agtttgtagg ttatgccaag
     1741 cctgtaagca aaggaacgtc aagatatcct ctttctgttg aagaagaact ggtattgaac
     1801 aggagcctga aaggtacaca aatatagaat aaaaaatata ccttcatagg atactaattg
     1861 tattgcacaa taagttgcta tggaggtgtt tttatgataa tcgcaataca gcatggcttg
     1921 gaagatataa aaaaagaact ggaaagcaga gggaatgaag tattctacat aggggaaaac
     1981 agaatggctg atgcagtcct ctacaaggag gcggatacac gtccttacta tgcagttaat
     2041 aatgttccct caaccgcggc gttatcaaac gaaaatatat catacggggc tctgttggtg
     2101 aatgtaacaa acaaatctat ggatgatata atcagaatat tggagaagcg aatatacagt
     2161 cctctgcttt aatgcatttc ataatttaca gcaggttagc tttcatatta atataaaatt
     2221 cccaaattta tgatataata tatattaatt gtcgaaataa atatgaaagg gtagcatgta
     2281 atgtatcagg gtgtatatga taggatacta ttaattgcaa tgacttgttt ttctttcttc
     2341 attgcattat ataccataag catgaacaaa acggtttcac ttttcaagct gctgggctgg
     2401 gtgtttttca taccctttgg attgtgctgc tcaatctttg ttttctctcc ggataccccg
     2461 ataaatctca tattatcatt ttcggagttt tgcttggccg caggcattct cctgcttata
     2521 ctttatcctt acaacacaaa aaatcaaaaa ctaatgattg ttgtaattgc tgctcttacc
     2581 ctgatactgt ccgtatatgc aataaaaacg gagcatttca tatactatac attctcagct
     2641 gctctccgaa atattctcac ggcagcatgc attattgctg ctattgtgtt caatataaaa
     2701 aagaatgcag ataggaaact tgctaagcca ttggctattt ggcttacggg tatgctgctg
     2761 ggatttgttg aaaacaaagc ttatataaat gaagcagtat ttgcagtcaa gttaagtgca
     2821 tatgcctgtt ttctattcta tttttttagg acaacccatg atttgcttat gaaaaaggtc
     2881 aatgaatctg aagaattgat agaagacatg aaaaagtccc ttaataaaga ggtaaagaaa
     2941 agagtatttg aaattgaaag aaccaatgaa atgctcttgg aaatatccaa gacagatatg
     3001 ctcacaaaga cgtttaataa gattacaata cttaatatca ttgaaaagct tataacaagc
     3061 aagaaaatgg aagccttttc aatattaatg tttgatatag ataacttcaa aacaataaat
     3121 gattccttgg gacacgttac cggagacatg tgcctgaaga ctctgtctgc tatcgcttca
     3181 gggaatatca gggaagtaga ctacatagga agatatggag gggatgagtt tattattgta
     3241 cttccaacct tgtcggtgaa tgaagcgaga tttgtggcag agcgcttcag gaagaaggta
     3301 agcgaaacaa gcaatcccag attcaccgta tcaataggca ttgcgacata ccctcatgac
     3361 ggacagaccg tacatgaact gatacaggct gcagacagag gattatacaa agctaaaagc
     3421 atgggaagaa acactgtttg ccgcgcatag tttattaatt cttattttcc atataatcaa
     3481 aaaacttatc ttcacttgct gtggaagcaa tttgccggtt tatcagcgag ttatcggata
     3541 acgcccttct gttcattttc cacatattgt atttaaggcc gtttgctata aggcttgcca
     3601 tgttcataac tacactgagc cttgtatttt gaagaatcat tatctccata aaaccgccta
     3661 catttactat ccctgtgata tttatatgac cgatagccgg aaggttctta ttgacacccg
     3721 agcccggaga caaggggcct tcctttatat ttatgaagcc tatcctttca tacctgccga
     3781 gacatgcatc aatggcaatg ataaagggat tgtcaaattt cctgaaaagc tccatatact
     3841 cacaaaggtt ctttgcatgg acaggctcat ccagagtacc ataaacatgt acatttttat
     3901 acctcatatc cttcacttta taccctatca acgggcccag gcagtcgccg gtagctctgt
     3961 ctgttcctat gcacacaatt acgatgtcgg tatggttttt atcatagaaa atactcatgc
     4021 attcctctaa gtaattgcag aaataagaaa aggaataagg tttattaata tcaactgttc
     4081 tctcatattt tatcttcaac attccgaact cctttatctg aaatatctac atagagtatt
     4141 gccaaaacac attgagtaaa ttcaaaggtt ttaaaattaa cagggtgtgg cgcaaagtgt
     4201 tttattgtat aattatatta ttcaatatca aatacaataa ccacatgttt tcaaacaact
     4261 gcatccaagg cctttgtgcc catatgatca atcaaataaa tcatgagttt ttatgaatac
     4321 ttttttggaa attattcgta tatattatga gaggtgcttg atttgcaaaa agtaagatcg
     4381 aaaaaaacaa ccgcattgct tcttctgctg atcatagtat cggtgcttgc cattctactt
     4441 tacataaaca acaaaacaac ccggaagtct tcttttattg caggcggaaa aagcaaactt
     4501 gctgttaccg gagaagtgac ggctgtatcg caaagcagca gcgccatata ctatgtgaag
     4561 gatcttctta ttgaagcaca ggcagaaaat atcagagcga agaatataaa tggagatatt
     4621 gcatggagct gcaaactgcc cgggaaggcc gtcaagatgg ttgatgcagg gcaaaatatc
     4681 ataattattg attcagccaa taacatccat tattatacac ttcaggggaa actcctttgg
     4741 acatacaagt cggattatga gattgtagat atttttacgg aggataacgg aagcttcctg
     4801 gcagaataca agggaatgac gggaagccat gctgaagcat tcaagcagga cggaagtaaa
     4861 attgggagta tatctgtgga caatgctcat atattatcat ttgcaggagg cagcggggta
     4921 ttttcgattt caattgtaga cacctcatca gaaatgatta agacaaaaat aataacatac
     4981 agctttaaag gggatatact gtgggctcaa aattttgaca atgtcattat ctcaaagctc
     5041 agcttcagta aggataacag gctgcttgcc ctgggagaaa atacggttta tatttataaa
     5101 aatgacggca gtcttcatca ggaagtcaaa attgaaggtg aaatcagccg tgcggcaatg
     5161 aacggtcata ttgtcgcatt ggcattggaa attgaagata agcaatacct ggtatgctat
     5221 gatgccaata tgcgggagca aagccgtacg gagataaagc ctgagccatt gggaatattt
     5281 ccgctgaaaa gcaatcttat agtatactat tctgatgaat tgatgatact cacccttaaa
     5341 ggagagctta atgcaaggta tgagcccaat gtggatataa gcgccgcata tatgacttcg
     5401 gataataagg tgtacatagc ttcaaacaga aggcttcttc agttggagta tataaaatag
     5461 cacaaaggag gccaaaaacc tatgaattgg attgactact tgataattgc aattcttgcc
     5521 gttggtgtca ttatcggctt caggaaaggg tttttgaagt cggtattcag cataatatgc
     5581 ctgttggtat caattatggt tgcaaaaact tactataaag ccttaactat gtttttggtg
     5641 gagaatactg cattggaaga gaagataaca ggttttcttt cagaaaaagc ttttgtaaaa
     5701 aacatattaa tgtctccttc gggggaaagt gcggtttttt ccatcagtaa cagctttacc
     5761 agcgatttga atgcatttgt aacagtactt ataataaatg caatatccgt gctgattata
     5821 tttattgccg tgagatttat tcttggaatt gccgaaagca ttcttttaga tgcagtcgaa
     5881 ttacccggac tgagagaagt taacagtata ggaggggcaa tagtcggcct tgcaaaaaat
     5941 atcataataa taatgctgat atttacaata ataactccta tttctgctat aaaggctctt
     6001 tcggggatct cagacggaat agaggcatct gcccttgcgg agtactttta tgcttataac
     6061 ttcatacttg gatggatttg gactgcagcc gttgaccttt tgaataaata gtcaagtctt
     6121 aaggagttgt tgaattcatg aacaagaaaa atgcactgat agggatagcc ctgatattcc
     6181 tgggcatatc catgtacctg aaaaactata atatcggaac aggcagcttg ctgactcttt
     6241 tccttgggct gggactgctt tatgcatatc atattaagag agaacagccc tttattatat
     6301 tcggaggaat ctttgcagct ataggcttgg cggcggtatt aagggatatc agattgttcc
     6361 gtattgatat ggtttttgaa gcaatgctta tagcgctggg aataatattt atattcttat
     6421 attactcaaa acatattgaa ggctttattt ttccgggggt gatacttccc gctgcaggag
     6481 tatacctttt attactgaga tcattcaacg acagatatgt atcaccaagc atattcttgc
     6541 ttcttggctt cgctttttat gtgatatacc ttatagccta tatggggaag tccaactggc
     6601 cgttgatacc ggcaaacata ctgctgatgg ccgggatatt tgcgtatgcc gtaagctttg
     6661 agataataac atggcatatg gtctatttaa agagggatta catctggcct ctgctgatga
     6721 ttggagctgg ggtattgata ctgataagca ggttcagaag aagggcttag catctagagg
     6781 acacggggac gtttcgagga catggggacg tttctcctgt cccgctgaaa gcgggacagg
     6841 agaaacgtcc ccatgtcctc gaaacgtccc cgtgtccttt tgttctaatt agatattcct
     6901 aatacttatc ggtgcatttg tggtatttgc cgtaattgat accttgttgg aggcggtatc
     6961 aaagttatcg cttttgagct gtacagagcc ctgcatgcct atgtttttct tctccgtttc
     7021 gtaagtgagg tttgcaagct ttacatcaat actgctcatt gcagtatgtg catcaaccat
     7081 atactcaaag ccttcttctc cggcaaggct gaaatcaatt tttccattag aggtgctgag
     7141 agtatagcaa gggttcagag aatcaccttt gcgggaagtg cttaccgcaa ttgcagcgtt
     7201 gctggttttt gcatctatat tgtccaggcg gctgtcctct acagctattt tggaatttga
     7261 ggttctgatg tttgcagttc ttgcggatac ttcgttaaag attatccttt cattgtctgt
     7321 aaatatttca atttcatctg cgctgcatct tgtcagaatt acccttccgt ttgccgtggc
     7381 acagcttata tgattagcat ctatatcact gatttcacat tttgcattgt ttgtagaaaa
     7441 gctaattaca tccatacctt tgggaagcag tatttcaatc ctcccccagg ttctgccggg
     7501 aaactcggtg gaaagcctgt aaccttcggt gtctttaatg accttgaagt attcatcaat
     7561 ggccagctga ggcacatttg aattaacata caggtttatc tttatttcct ttgtatcaac
     7621 gggacaggct tttacggaga aattacctgt ttcaaggtat aagctgagag tgtcttccac
     7681 cgggtaggta tagctgctgt tatatttatg cttctcgtac actgtgtcaa agagattgtc
     7741 tatctggctt gtaaagacat ctgcaaattt tgctgcagac tcagcaatat cctggcccag
     7801 tatttccatc ctttttgtga attcctcggc aaatttttca gcattatttc ctgtcttatc
     7861 ggtgttaata ttctctccca gctgctctac cagctttcct gccttttcgc cgaaggtgga
     7921 agcaagcttt gaggcttttt caccaaattt gttcagtttc tcatctattt tgatttcgtt
     7981 aagcttggca gcaagttcat cttttatttc gtttattttt gaaccggagg acggtgtgcc
     8041 ggagccgccc ttctccaaag cttcaagaag ctttacagct tcatcggtgg ttattttccc
     8101 ctcctggagc attttaagta ttaacatttt ttcttccaca atatttcccc ctattcactc
     8161 attaatctga ttgcttcttc cgaggatatt tcacccctat aaagcctttc aagaatttct
     8221 ttcttatagc tctgatccac actctgactc ttataaccca atgccgaaat gagagaatcc
     8281 agctttccct tcactgtcgg gtatgatatt cccagctccc gctcgatttc ctttatattt
     8341 cctctatttt taatgaatat ttccgcaaat tccttgtgct cctgtgataa ggaacagaat
     8401 ttacacggct tgaagtgccc ttttatcgtg cttttgcatg aattgcagga cacttccgtt
     8461 atctccaccg gtccgctgca tataggacac tgccccatga cttcacgttt cattacttca
     8521 ctcctaacta ttaataatgt attgccgtta tatcccatta tagcccaata attaataatg
     8581 tcaatatata aattaacatt attaatttaa tttattggaa atattaaaaa atagtaaacg
     8641 catataaata ttattaataa atatggaaag cctgtataga atctctgctt atttatatat
     8701 acgcaaaaca tgctgcttta gttttaaaaa ttaataatta atgatataat tcaaatatta
     8761 taacaagatt ttaatgaggg tttttgcaca cttgcaaaaa cctacccgag cgtttcaacg
     8821 aggcgggaga tatgcttacc gcctgttggg caacgaacgc ggtatccgcc atctataaag
     8881 gcgggagaca tcattagcct cgttataaca gaaatcatat cgcaccatag agtgaggagg
     8941 ataagtatga aagaaataga tgccagaggg cttgtgtgtc ctcaaccggt aatactcacc
     9001 aaaaaggccc ttgaagggat gacggagggc gaggtagtaa ccattgtaga caatatcacc
     9061 gcaaaggaaa atgtttcaag attggctgaa aatttaagct gcaaatatga gatatccgac
     9121 gaaagcggct gttattacat aaaaataata aaggcagcgg gagcagaaag tacggaaaca
     9181 aaagaggaca gcacagtaat agtaataact tcggacaaat taggccaagg agcagaggag
     9241 ctgggaaagg tgctgataaa gagctatact tacgcactta ccgaagccac accgctgcca
     9301 aaggctgtga tgtttttaaa cagtggtgtt aagcttaccg cagagggctc agaggtggcg
     9361 gagaatatta agaagctgga aaactcaggg gtggagatta taagctgcgg tacctgcctg
     9421 gatttctatc agttgaagga taaactccag gtcggcatag tcggaaatat gtattcaata
     9481 attgaaaaga tgaattctgc cggcaaagta attaatatag gataggatgg atgcatatgt
     9541 attacccgaa acaattcaac ataggagata tcgtggaaac aaaaaaacag catccctgcg
     9601 gcgataatca ctgggaagtc ataagggtgg gagcggattt caagataaaa tgcctgaaat
     9661 gcggcagaat agtgatgctg ccgaggccta agtttgaaaa atctgttaaa aaggtaataa
     9721 aaagtgcagc acctgaagag aataatattg attaaagctt tatatagtgt tataatctat
     9781 aattgcgtaa atatcctcgc tctatcatat tcagataggg ccgttagtcc gtggggagga
     9841 ggtgaatttt tatgaacagg tacgaaacca tatatataat caggccgacc gttgatgaag
     9901 aaggtataaa agctcttcag gagaagttca aggcaattgt tgaagaaaac ggtgaaatcg
     9961 aagctataga tgactggggc aaaagaaagc tcgcttatct tatagacgat atcggggaag
    10021 gctactatgt atatatgaaa tataaagcag cgcaggttgt accaaaagag ctggacagag
    10081 tattcggcat aacagaagag attttaaggc acatgataat aaaaattgaa gagtaggaaa
    10141 aaagaatcca aaagaaaaaa atttaggcgg tgtgattatg ttgaataaag ttgccctggt
    10201 gggcagattg actagggatc ctgaaatcag gtatacagca gataatcaaa caccaatagc
    10261 taaattcact atcgctgttg acaggaccta taaaagggaa ggtcaaccaa ctgcagattt
    10321 tataccgata attgtatttg gaaaatctgc ggataattgc ggaaagttca taaagaaggg
    10381 gaggcttgtt gccgtttcag gaagactcca gacaagaaac tgggatgatc aggatggcaa
    10441 aaggcattat gcaacagaag tgatagcgga cgaagttgac ttcctggaca ggggctctga
    10501 tgcaagggga caaggcaacg aaggcagatc tgattcggat tatggtgatt tccacctggc
    10561 tgaagaagag gatgagcttc cgttttagca gatatgttac ataaaacata cagattggag
    10621 ggaaaatgat tgaacagacg tagtggcggt gaaagaaccg gcggcggaaa aatgagaaag
    10681 ccaaagaaaa aagtttgtaa cttttgtatt gataaagttg aacatataga ttacaaagat
    10741 gtaaacaagc tgagaagatt catctctgaa agaggcaaga taattccgag aagaatttcc
    10801 ggaaactgcg caaagcatca gagacagctc acaatagcaa taaagagagc aaggaacata
    10861 gctttcctgc cctttactac cgattagaag aataaatatt ggactcggag gttttttgcc
    10921 cccgggtttt attttttgga ttatgaaatg ctattcaata ccgctgattc caatatttaa
    10981 aaggattttt cgggaaggta ttatatttag tatgtcgaat taaaaaacag gttaggctgt
    11041 ttggcattgg aaggttatat ggcttgcaaa tccagtggtt ccaaagatta caagacattt
    11101 tgatttgttg tttactttga cgaattgtgg taatataaaa taagcttccc gaacatgatt
    11161 tgactaattt tttattatat atggaatata atattaatag tggacaaggt caaataatgt
    11221 aatgggggga tcagcttgtt aaaccaggac actgacatta caaaaaatat aaaaatgata
    11281 gagtttttga aaagcgaact cttaaccacc gttgcatcac tgtatcagaa tctgctgaaa
    11341 ggtacaaagg tggggcaggg ggtcattcag gatatactgg caaaccttgt actggttaca
    11401 tatctgcttg gtaaaaggct tggagtacca tattcaatga ttgataccaa gatactggaa
    11461 aagatacggt taggaatgct ggaggagcac gagacggaaa aatggtacgg ggacctgact
    11521 gaccttggcg attatttgaa aacgaagaaa ggctagaggc tcagcatgga gagaatgcaa
    11581 acaagggcac tggtagaagg tgctatattt gcaggtgtca ctgcactttt gggaatactg
    11641 tattattata cgcagtatct cggaataata gctatggttt ggcctgtacc ggtaataata
    11701 gtgggctata gaaacggcat taaagcgagt atactgtctg ccttatctgc cgggttggtg
    11761 gtttctctgg tgacgcagcc tctggtaggg gttgggcttc tgattggttt tggtgtcccg
    11821 ggagtattaa tggggcatat gataaacaaa agggtgaatc cgtatgcagt aatattagtg
    11881 tgcggtgtag tactgtccgt aagcatggcg ggagaatttc tgctgtcatt aaaggttgca
    11941 ggtattgacg gattggaatt tttatcaaat cttgattctg ccttcaggga gcagttggaa
    12001 acatccttga atatatatag gcagttcggt gctgcagagg aaaaattgca ggaattcaaa
    12061 gattacttta gtcaggcaat agagctgatg aagtatgtga ttccttcttc attgctgata
    12121 tcaggcgtat ttttctcatt tattgattat aagcttacaa gactaatact gaaaagaatc
    12181 ggccttattt tgcccgacat agatgaattt tcaaaatgga ggcttaccga accatattca
    12241 tacatattgc ttggaacggc tgttcttgca ggggtagcat catactttaa ggttcccgga
    12301 ttcaatgcag tcgttttaaa catctctact atactatcac tgattttctc tattgtcgga
    12361 atatcggcag ttgtttattt cgcaagggtt tatggggata gatacggtat cccgaaagca
    12421 ttacggagaa taataactgt ttttgtcatt cttaccttta ttcagtttat agcctttatc
    12481 ggcatattag atatggcgtt tgattttagg cggctgaaat ccaaaaatcg cattggaggt
    12541 gtgagatgaa aattcgcaag ttttcggaca tatgggggcc ggatgtgagc atatctcttt
    12601 ggatactcgc cattgccgta gtagtcatag gctattacaa tgtttttttg ggtttggccg
    12661 gcgcactgct tctgggatat ctggtttacc acaatcttca tgccgcaacc agaaaaaagg
    12721 aagaactcag attatatata gaaaacttat ctgaaaatgt agatactgca accaaaaatg
    12781 ccattcttaa cctgccgttc ccattggtta ttattgatga tcagggcgtt atcaattggt
    12841 ataatatgct tttctcgagt attttccaag gagagtacat acttgacaag aagctgtcgt
    12901 cctatttaag cggtgtggat atcagcagga tactgaagaa taaaatggaa aggttcaaga
    12961 atatcaatat caaagacaga tactatgata tatactgcaa tttgattaat atcaatgaaa
    13021 gcggcgagga aaagaatata ataataatgt attttgtgga taagacagac tttatcagcc
    13081 tcaggaacag atataacgac gaaaagccca tagcggctat acttcaggtg gataacatag
    13141 aagaggtaat taaagagaca gaggaagcgt taaggcctgc cctggttgcg gaagttgaca
    13201 gaagggtcaa tgcatgggcg gcacttaaca acgcatccat ccagaaatat tccaacgggg
    13261 aatacatgat ttatttcagt cataagcagc tgatgtcaat ggaggaaaaa cgttttgaca
    13321 tacttgacag catcagggaa attaattcag gcaacaaaat tcccataaca ttaagcatag
    13381 gcgttggagt taacgggaag aaccatgcgg agctgcagaa gttctgtaca agcgccatgg
    13441 atattgctct gggcagaggg ggagaccagg cggttgtcaa ggataatgaa aagctttcct
    13501 tttatggagg caagacaaag gccgttgaga agaggacaag agtaaaggca agggtaatag
    13561 cctatgcatt aagacagctt attgagcagt ccgaggatgt gcttattatg ggccatgaaa
    13621 tgcctgacct tgactgtatg ggttccgcct tgggcatata caggtgctgc aaaggcctga
    13681 acaaggaagt gaaaatagta cttggcggtg taaatgcagg tatagcaaac ctatggtcgg
    13741 agctgcagga ggatgaggaa tatgcggatg tatttgtcag cccgcaggat gcccttcaaa
    13801 tttgcggggc aaatactttg ctggtggttg tagatgtgca caggccggca tttacagtgc
    13861 aaaagcagct tgtagaaaaa gctgagaaaa ttgcaataat agatcatcac agaagaggca
    13921 gcgaattcat tgagaatgcg gttatcacat acatagagcc ctatgcatct tctaccagtg
    13981 agctggtaac cgagttgctg caatacatgt ttgaaaagcc gaagataaag cagatagaag
    14041 cggaagccct gctcgcaggg atatttatag ataccaagaa tttctctttc cagacagggg
    14101 tcagaacctt tgaggctgca tcctatctta gaaggatggg agcggatact accctgacaa
    14161 agcagctttt ccaggatgac atggcaactt ttttgactcg ggctgaggta gtaaagggag
    14221 cgcaaatgtt caagagcaat atagcaattt ccactatacc ggtgaatgca aagaatcctg
    14281 ctctggttgc agctcaagcc gctgatgagc tgttgaatat caaaggtgta aatgcttcct
    14341 tcgtactttc ctgcattgag ggggatattt tcataagcgg aagatccctg ggagatatta
    14401 atgtgcaggt ggtgctggaa aagcttggcg gaggcggaca tctcactgtg gccggcgcac
    14461 agcttccggg gcttactatg gaaaaggcca tgagcttatt gaaaaaagcc atagaggaat
    14521 atcttaagga aggtgaaaga taaatgaagg ttgtactttt acaggatgtt aagaatctgg
    14581 gaaaaaagga acaattggtc aatgtcagcg acggctatgc caggaactat ctgttcccga
    14641 gaaaacttgc agcagaggct acttcaggga agcttaaaga actggaggaa aagaaaaatt
    14701 ctgaaataat aaagaaggat aaagagaaac aagcagcaaa ggagcttgcc gacaagcttg
    14761 aaaaattgga ggtaaacttc aaggtaaaag cgggagagaa cggcaaattg ttcggttcca
    14821 taacaagcaa ggatgtggct gatgctttaa aatcacagca caagacagag atagacaaga
    14881 aaaagatagt gcttcatgat gccattaagg cattggggac atataaggtt gaaattaagg
    14941 tatatcccgg gatatccaca ttaataaatg taaaagttga agaacagaga ggggtctaaa
    15001 aggtgagcca taaccatagc agcatacgca gcaaagatgt atcaattgac gtacttgttg
    15061 attttacaaa aaaattgtat tctagtgata agaccagaaa actggataat gttacagata
    15121 ataaagaaaa gatttatata ctgcatgaaa tagtttccgg caaaatagaa aacaagagaa
    15181 tagaagagaa caagctggaa tttttaattg aagatattaa ggacaagctt tctgatgccg
    15241 cagccaaaag atacatagaa gaaggcataa aacgggaagt cataaagcgc atggaaacaa
    15301 gggaaataga atacttgaat gaaatgaagc tgcaggttat aaaaaagcat gcagggcttg
    15361 agaatgcttc taccctgaaa aaatatgcgg acttggagct gatgaagaag gtaaggctta
    15421 caaaatcaat aatggatgta atgcgccctc aaaggcagga tgaaataatt ggacaggaga
    15481 atgctgtttc agctttgata tccaagatag cctcaccctt tcctcagcat gttatattgt
    15541 acgggcctcc gggcgtaggc aagactacca cagcccgtct tgcccttgaa atcgcgaaaa
    15601 gccggaaaca tactcccttc agcaaaactg ccaagtttgt agaagttgat gcaaccaccc
    15661 taagatggga tccgaaggaa tcaataaatc ccctgttagg ttcggttcat gaccctattt
    15721 ttcagggagc caacaaggag cttgcagaaa tgggtattcc tgagcctaaa cccggcctgg
    15781 ttaccgaagc tcacggaggt gtgcttttta tagatgaaat aggtgagttg gatgtgatgc
    15841 tccagaacaa gctgcttaag gtgctggagg acaagaaggt aaggtttgat tcctcatatt
    15901 atgatgtcaa taacgaaaac atacctcgat atataaagct tctgtttgaa gaaggtgctc
    15961 ctgccgattt cattcttatc ggagcaacca ccaggcagcc ggaagaaata aacccggctc
    16021 ttcggtcacg ctgtgcggaa gtgttcttca atcctttaag cagttccgat attgccagaa
    16081 tagtattgaa tgcggctgat aagcttggtg cccgaataag tgcaaagggg gcagagttga
    16141 tcagcagtta tacatacgaa gccaggaagg cagtcaatat tcttgctgac tgctacagca
    16201 gcatgataaa tgaaagggaa gacaaggata tagaaatatc cgttgattgt ataaaggaag
    16261 tattaagcaa aggcagatac gtgccgttga acaagaaaaa agcggagggc atcagagaaa
    16321 tcggacggat taacggcctt ggagtgaacg gctttatcgg caatgtaatc gaattggaag
    16381 cagtatgctt tgccagagaa gatgaagcgg gcaccataag gttcaatgag acagccggca
    16441 gtatgacaaa ggattcttta ttcaatgctc tttcggttat aaggaaaata acggataaaa
    16501 atattaacaa atatgatatc catgttaact gcgtaggcgg cgggaaggtt gacgggcctt
    16561 ccgcaggagc tgcaataacc tgccttttgt attcggcaat attggagcgg cctgccagac
    16621 aggatacctg cattaccggg gaaatatcca taagaggccg tatcaaggca gtgggcggca
    16681 tcgttgaaaa aatttcagga gccaagagag caggcttcaa gaatgtcata attcctgaag
    16741 aaaacaagga agaagccaac ggcctggatg gaataaatat aattcccgca ggcaatattc
    16801 aagaagtgat cggttttatg tttgatgaac aatagattct aggagtggat gaaatgagtg
    16861 aaatgataca gagaataccg cccaacaata ttgaagccga acaatcggtt ctgggagcca
    16921 tgctccttga taaggaggct atatccacag ccacagagta tatttcagga gatgactttt
    16981 acagggaagc acataaggag atatttgagg caatagtgga tatatacaac aggggggagc
    17041 cggtagacct tattacactc actgagaagc tgaagaccag aaatacattg gaggcagtgg
    17101 gaggaataac ttttctgacc aaccttatgg atgctgttcc gacgactcac aatgtcaagt
    17161 attatgccaa aattgtagaa gacaagtctt tgcttagaaa gcttatcaaa tcatccaacg
    17221 agataatcca gaagagctat caggctgaag aggatatagg ggaaattatt gatgatgctg
    17281 aaaaaggcat atttaatata tccctgaaaa ggtctaccca aggctttgtt catctaaaaa
    17341 acatacttaa cgcaaacttt gacagaatag agcaattgta tctgaacaag ggaaaaatca
    17401 caggagttcc taccggcttt catgatctgg acaataagct ttcgggccta cagaagtctg
    17461 accttatact tattgccgca aggccttcta tggggaaatc ctcgtttatg atgaacatag
    17521 tccagcacgc agcggtaagg gaaaaaacta ccactgcaat attcagcctg gaaatgtcca
    17581 aggagcagct tactcagagg ctgttgtgtt cggaagctct tattgatgct caccgcttaa
    17641 ggataggcga tatcaatgag gatgaatggg taaagcttgc aagggcaatg ggccctcttt
    17701 cggaagcacc tatttatatt gatgataccc cttctatttc tataaccgaa atgagggcaa
    17761 agtgcaggag gctcaagctg gaacatgacc ttggacttat tgtaatagac tatttacagc
    17821 ttatgcaggg gaaaggctat gccgaaagca ggcagcagga gatatctgaa atatcaagat
    17881 cactaaaagc tttagccaga gaaatcaatg ttccggtggt agcgttgtcc cagctttccc
    17941 gcgcacctga aatgagagca gatcacagac cggttctctc agatctgaga gagtcgggag
    18001 ctatagaaca ggatgctgat gttgtaatgt tcctatatag agatgaatac taccatcctg
    18061 atacagaaaa aaagaatatc ggagaaatta atatttccaa gcagaggaat ggacccaccg
    18121 gtactattga actgatttgg ctggggcagt tcacaaaatt tgtgaacaag gaaaagcata
    18181 taacagcaat ttaatttaca taattataaa attttgttgt tttattggag ctttcgtagt
    18241 attttgtaga aggctccttg ttttctaaaa cttgaacatg gttttgcctg tggataacct
    18301 ggggatattg tgaataatta agttgctcga ttaacataaa ttctgaatga taattatgca
    18361 cggcagtata tacattgaga caagagaatt tgcacagcca gccgaacata tgtgcatatc
    18421 tctgtggata atgtggataa tgtgaatcag aattttcaga aaactagttt ccgcattgcg
    18481 ccaacgctgc tattaaaatg aaatacacag ttccataatt attaattaaa atccatataa
    18541 taatacaagc aggactaata ataacaatct tcaagcttct tagagtataa ttttcattgg
    18601 caaaataatt ggcaaaaata aaggaagagg caaaacaccc cttcccatca tacaaatcaa
    18661 actgtaaaat gtaattgtca agaaaacata gacaggagtg atttgtatga ttgatataat
    18721 tgtaaatgaa aaggatttat cattcaatag gttagaaaag gaaatttatg cttatggttg
    18781 ccagatagct cgcgaaattc ttaaaagtgt tttagaggca atggataaga ggctcgcaga
    18841 agaaagagat aaatcaaaat atagacatag agggggaagg aacacatcaa taaaaactct
    18901 tatgggagaa gtggaatttt cccgtgtgat ttatgaaggt aaagatgatg aagacagaaa
    18961 gtcattcata tatttgttag atgaactttt aggattcgat acaatagggc agatatcaac
    19021 aaacctggcg gaaaagatag tagaaaatgc cagcataaca tcttaccgca atgctgcaga
    19081 taatataact gagctaacag gacaaggcat cagccatggt ggcgtatgga atgtggttca
    19141 ggctttaggg caaaaggtaa gggaagaaga aacagaactg gctaaagcag cagagaagaa
    19201 tcagttgtgc gggggaaaag aagttccggt tctttttgaa gaagctgacg gggtttatat
    19261 aaacatccaa ggtaaagacc gtccgaagtc aggtaagaag cttgaaatga aagtagccgt
    19321 agcatatgaa ggctggaaag aagttagcaa gaatagattt gaactggtaa acaaagtagc
    19381 ctgtgcgggc tttgaggata tatctgagtt ttacaaaatt aaagaagcga tgatatcaag
    19441 agaatacaat gtagatgaga tacgaatgag gatacttaat ggtgacggag ctgcctggat
    19501 aaagcatggt atagatgat
//
LOCUS       DAOOAB010000052        18541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_3969_length_18541_cov_15.349454, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000052 DAOOAB010000000
VERSION     DAOOAB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 18541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18541
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_18541_cov_15.349454"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..902)
                     /locus_tag="PKU88_10220"
     CDS             complement(267..902)
                     /locus_tag="PKU88_10220"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HPX69689.1"
                     /translation="MLIVKGVLKHLTPKQRKIAEYWGDGPATKQWTPIIDRLLDTYGF
                     SPVRAARVLAAVQAGINDAFVITWYFKYLWDVPRPNQLDQRLVTALCTPKFPSYPSGH
                     SVISGTAEVILSYFFPPEAERLKELAEENSISRLYAGVHFIEDITEGLRLGRQIGRII
                     VEELMRQSDENQSGIDIPITESRNADLLPPPYEQVIAYPSRVKDCDLPLLP"
     gene            complement(1135..1602)
                     /locus_tag="PKU88_10225"
     CDS             complement(1135..1602)
                     /locus_tag="PKU88_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPX69690.1"
                     /translation="MKAEYINSFYKATKEVFKLMVGIDPQKKDLRVIEDMVSSKDASV
                     VLGVTGDLRGTILFGFPKDMTLEMIRIMSGMEMDRIDSFASSALGEVANIISGNAMTL
                     LAQNNYACDIVPPQIFIGEYKSFTMAGERALQLILTTPIGEFDINISLKEKQV"
     gene            complement(1602..2021)
                     /locus_tag="PKU88_10230"
     CDS             complement(1602..2021)
                     /locus_tag="PKU88_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HPX69691.1"
                     /translation="MMWKDKYSIGIELIDEQHKELFKRVSDFLRTVESKDNWEDKLEK
                     VKETMAFMQEYVIVHFDDEEAYQEQIGYPEIENHKIAHLKFKEGINDYVRIFEEEGFN
                     EEKIQEFGGKLMAWLIMHVGKMDQMIGEYAKSKGGEA"
     gene            complement(2192..2899)
                     /locus_tag="PKU88_10235"
     CDS             complement(2192..2899)
                     /locus_tag="PKU88_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69692.1"
                     /translation="MLKVKDLHVYYGGIHALKGINLEVNEGEIVSIIGSNGAGKSTLL
                     NAISGFLKYKQGEISYNDNKLGFNPSKIVKTGICQVPEGRLVFANLTVKDNLYMGAYL
                     RNDKNEIKEDLEKVYSLFPRLKERENQVAGTLSGGEQQMLAMGRGLMSSPQIMLLDEP
                     SLGLAPLLVQTVFDIILDIRKMGKTILLVEQNAYKALSIADRAYVLEQGKISASGQAS
                     DIASDPKIQEAYLGVKK"
     gene            complement(2902..3666)
                     /locus_tag="PKU88_10240"
     CDS             complement(2902..3666)
                     /locus_tag="PKU88_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69693.1"
                     /translation="MGILELHGLTKTFGGLTAVGDVDFNVEKDSITGLIGPNGAGKTT
                     IFNLVTGVYKVTSGKIIFDDKDITNKEPYLIAESGITRTFQNIRLFKKLTVYENVLTA
                     CHYNANYSLLDSVLRTPKFRMQEKKLNEQAAELIAIMGLEDRKDLIANNLPYGLQRRL
                     EIARALALMPKLLLLDEPAAGMNPDETEQLMKLISEIRKKFNLTILIIEHHMDLIMGV
                     CENIMVLNFGCKLAEGTPKDVQNDKSVIEAYLGVEV"
     gene            complement(3668..4564)
                     /locus_tag="PKU88_10245"
     CDS             complement(3668..4564)
                     /locus_tag="PKU88_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPX69694.1"
                     /translation="MGNEYILGILTLAGINLMTVLGLSLLTGFTGLFSFGHAGFMAIG
                     GYATALMTVKFHWPFIPALLAGGLAAALVSAALGKVTLNLKGDYFCIATLGFGEAIRL
                     ILDNVQTFGGARGWPGIPHYTNVWNVLGINIVAVIVLANLIRSRHGRNMIAVREEELA
                     SQIVGINVFNYKMTSLVTSAAYAGIAGGLMGHYMTFLQPKMFSLAKSTELTIIVIFGG
                     LGSISGSVLGALVLTSLPEILRAFSSWRLVFYGLAVIFIMISRPEGLMGGYELTPSSM
                     KKLFRNMAAKRENKLQRAGGGK"
     gene            complement(4577..5461)
                     /locus_tag="PKU88_10250"
     CDS             complement(4577..5461)
                     /locus_tag="PKU88_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPX69695.1"
                     /translation="MTQFFQQLINGLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIM
                     FGAYFGFFFLTFLRFPFPLAFISAAICSAILALIIERVAYKPLRERRAPFLYFIISAM
                     GASIFMENLVIAIPAIGPTFRTYPEVFNKQPIKIGELAMGKLDIAMFLISAICLIILI
                     YIIDHTKIGKAIQATSYNLKASALMGVNTDFILITVFLMGGFLAGIAGVLFGMKYTVY
                     PQIGIITNKSFIAAVFGGLGSLPGAVIGSVLLGIIETFTAGYLSSQYRDLIAFVLLIT
                     VLVVRPMGLMGKVTEDKA"
     gene            complement(5573..6754)
                     /locus_tag="PKU88_10255"
     CDS             complement(5573..6754)
                     /locus_tag="PKU88_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX69696.1"
                     /translation="MKKKVLALLLVCLMAVSAGLAGCSPQTAAPAEPAAPAEEKILVG
                     WMGSLTGDQAVWGQCELNTVKMLFEEINAKGGVLGKQLEVIGYDTKGDPQEAVNVAKR
                     LTTQDKVCTILGPNASGNAIPIAPVLEEAKVPDIATVATNPKVTVLDGKIKPYNFRVC
                     FIDPYQGAVAAGYAADVLKAKTAAVLYDVGDDYSQGLTEFFEEFFVKKGGTIVAKEGF
                     KTGDVDFRPQLSKIKAANPEVIFMPYFFKEVALSSNQARELGINATLMGGDGWPSEQL
                     ISMAKDAVEGSFFVNHLDYDDPEVQDFKTRYKAKYEKDVELNGYLVHDAVLMFVDALT
                     RAGEANGEKIAQALETTDIKGITGRIKISKDTHNPEGKDAAILKIVDGKYVFQQKFAA
                     E"
     gene            complement(7136..7726)
                     /locus_tag="PKU88_10260"
     CDS             complement(7136..7726)
                     /locus_tag="PKU88_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69697.1"
                     /translation="MPSTFRNEELVKVQGKIYPVVGGRLRLAHDENENLSIETSVVQY
                     NEESTVVQAAVKTGKGTFNGLGNASVKRDKVLSNAILELAETRAIARALRFAGYGVEY
                     TGFEEVGEGKSISEENQSQQAEEHKNYEGATKTQINTIFAIAKNLNISNDELRDLIQD
                     KTGKSFSKDLSKSDASKIIRMLKERDVVENQGTKLM"
     gene            complement(7782..9395)
                     /locus_tag="PKU88_10265"
     CDS             complement(7782..9395)
                     /locus_tag="PKU88_10265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX69698.1"
                     /translation="MRLTFCGAAGTVTGSCHLIETGNKKILLDTGMFQGGSEAEEMNF
                     EPFRFNPSEIDMLILSHAHIDHSGRIPLLVKQGFKGKIISTAPTFELCKIMLPDSAYI
                     QESEAEWKSRKRKRAGKGVAEPLYTVKDAEASLKFFQPVEYNKIINIDPDISFRIMDA
                     GHILGSGIIELWIREGSEEIKLVYSGDIGNKDAPIMNDPTIIEEADYIIMESTYGDRL
                     HMEMNNKTLALLDIITDTIERNGNVVIPSFAVERTQEILYILNMLKECKSLPLCNVPV
                     FVDSPLAINATNIFQSFLPYMDEDTQKLLKEGDNPFDFPNLIFTHTAEESKAINSMEG
                     SSIIISASGMCEAGRIKHHLKHNLWREESSVVFVGYQAKNTLGRRIKDGEKLVKIFGE
                     EISVNCNVYSIEGFSSHADQRGLIEWVGSFIKKPKKVFLVHGEEESSKVLSKLMESEL
                     GIPVEAAKLGETLELSSSAAREITVERRSMNKHNLISYLRALKAASGDTFDSLEYIIS
                     EGSNTNNDSLLYHLKKLEESIAELKKEIS"
     assembly_gap    9456..9555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9893..9969
                     /locus_tag="PKU88_10270"
     tRNA            9893..9969
                     /locus_tag="PKU88_10270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9927..9929,aa:Pro,seq:cgg)
     gene            complement(9976..11064)
                     /locus_tag="PKU88_10275"
     CDS             complement(9976..11064)
                     /locus_tag="PKU88_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HPX69699.1"
                     /translation="MLSQLESKCTSDIDQKEMRTLAVVLNHYDFRVESIDKVRSAYKV
                     GTDKGFFCLKRVNNGYEKAKKSYNIMLHLKENGCDYIAEYYFTKEGKPIIKRKGTAFY
                     LTYWIEGCEASFSIPDEMERYSELLADFHNRAKGFRTPKHVKVKSHIKKWRRTFAKCR
                     DELAGYREYIDKLKLKAEFDYIYKSNIDYFIREAELAIRILDHSRYNELCEYYINEGS
                     ICHDSFYYQNIIVDRNNKLFVVDLESCQYDIPMSDLGKMIRRILSKKRFKWDFDLCRR
                     LIASYCKLRPMSKEEYEMLLAILVFPHKFWKLGRKRYIANKKWNEKKYLKKLKRQLRN
                     REYKREFIYCYINFYGLDLDYDPDIIEL"
     gene            complement(11197..11979)
                     /locus_tag="PKU88_10280"
     CDS             complement(11197..11979)
                     /locus_tag="PKU88_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPX69700.1"
                     /translation="MLENYEGFKQKILKLTGIDLSSYKERQMRRRIDSLIHRNNYDDY
                     DEYFKALTQNSNLYDEFINYLTINVSEFYRNPEQWKVLENEIMPEIMKFNKKPKIWSA
                     ACSTGEEPYSLAMMMSRYVDLNSFRILATDIDDAALNKAMVGIYSAKSLENLPRNFVD
                     SFFVKSGDYYKVSERIINCIDFRHHNLLVDKYPENCDLIVCRNVMIYFTEEAKITMYH
                     NFRNSLSNNGVLFVGSTEQIILPYKYKLESIKTFFYKKSGQP"
     gene            complement(12212..14503)
                     /locus_tag="PKU88_10285"
     CDS             complement(12212..14503)
                     /locus_tag="PKU88_10285"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HPX69701.1"
                     /translation="MKIIIDKYAGFCFGVKRAVDKAFEEADKKKDRRIFTFGPLIHND
                     QVTGKLEELGIKSADSIDEINCEGAELIIRSHGIGEEEYRQIEEKGFGYIDCTCPYVS
                     AIHKKVQEYYKKGYKIFIAGDKAHPEVIGINGWCDNSAHVIEDEKTVDKLPIYDKICL
                     VAQTTITKKIWDSVVDAINKKYKNAIIFNTICSATEKRQESAEQLSKKVNCMIVIGSR
                     KSSNTNKLYQICLENCDNAYHIEKAEDLPAGMLKGFETVGITAGASTPDWLIKEVIEK
                     MQDNGNIMGDPVEIMENQESVVDDGEEMEKQEDTTEDQGNMMEEYEKTMRPVYPGDTV
                     KGTVIMVTDDEVMVNIGYKSDGIIKKEDYTWDTDVSLKDIIKAGDELEVMVVRINDGE
                     GNVVLSKKLLDAEKNFNKIEAAFNEKTPLEGFVKEIVKGGGIVELMGVRAFMPASQFD
                     VRYSEDLSSFVNKKVNVEIIEFDPQKRKVVVSRKSLLKKQNEIKEKALWENIEVGQRL
                     TGEIKRLADFGAFADIGGVDGLIHISELSWSRVKHPSEIVKPGDKVEVIVLSVDKEKK
                     KISLGLKQTVPEPWSAAGEKYKPGDVLDVKVLRFSNFGAFVELEPGIDGLIHISQISD
                     KRIAKPSDALKIGEIVKAKIVDIKPEEKKISLSIRELIEAIGEEAKEEAEKPVIEEAK
                     EEAAEKPVIEEAKEEAAEKPIVEEVKEEAEKPAVEEAKEEAEKSVIEEVKQEAAEKPI
                     AEEAEEAAAENITEETAEETAEE"
     gene            complement(14487..15086)
                     /locus_tag="PKU88_10290"
     CDS             complement(14487..15086)
                     /locus_tag="PKU88_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPX69702.1"
                     /translation="MFYRFARSLLIPVFLLFYNYRITGRENIPKDGPYIVCGNHVSAV
                     DPILVGISIPNRMYFMAKVELFRNRLLRALMNALGAFPIKRGEADIKSIKTSLKLLNS
                     GKILALFPEGTRKKSDGITAEPGIAMLAIKSKVPIVPVAIISSYKFFKRTNIIIGEPI
                     ELAEFYDKKLLNDEYIKISVDIMKRINELKKDRENENNY"
     gene            complement(15106..15753)
                     /gene="cmk"
                     /locus_tag="PKU88_10295"
     CDS             complement(15106..15753)
                     /gene="cmk"
                     /locus_tag="PKU88_10295"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPX69703.1"
                     /translation="MSGYKVIAIDGPAGAGKSTIAKKLADMIGYTYIDSGAMYRAITL
                     KVLRESIPLKKTDMLISMAEKTDIDFRDNSIYLDGRAVNTEIREEDVNRNVSYIAAIS
                     EIRKLLVDIQRKISMNKNVVMDGRDVGTVIFPEADVKFYITASVEERADRRYKELKQK
                     GYEAEIQDIRSQIEKRDYIDSTRSDSPLKAASDAIIIDTTGKGIDEVLNEVVSYI"
     gene            complement(15859..16317)
                     /locus_tag="PKU88_10300"
     CDS             complement(15859..16317)
                     /locus_tag="PKU88_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69704.1"
                     /translation="MNSSFQSWWDRIDGLIRKVLLLLFAALIISQALLLSQTIKNIIS
                     RIDKLEGRSIADSQLFIKRGELEISIENYSTLKPLVFYINGKSIDTPNGRSIRLKVKD
                     SDIIEVGGGPLNDTAVLKVTAVSDSITVPEPGKLVYVKDNLVMVDRIRIK"
     gene            complement(16382..17614)
                     /locus_tag="PKU88_10305"
     CDS             complement(16382..17614)
                     /locus_tag="PKU88_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPX69705.1"
                     /translation="MIYDVIVVGGGPAGMIAAGTAASKGKRTMLAEKNEKLGKKLFIT
                     GKGRCNVTNTADFDEFMKNIPKNSKFFFSSFSSFSNNDLILFLESLGLKTKVERGGRV
                     FPESDKSNDVIKALERYLNRNKVDVRLNSKLLDIRQENGAVTGALFDNGRVVECSSII
                     ICTGGLSYPNTGSTGDGYKIAVKAGHSIIEPRPSLVPLVSDDAFVKELQGLSLKNVAI
                     KAYAGGKLLYEDFGEMLFTHYGLSGPIILSASFFISDYLRRKQEIRISIDLKPALSEE
                     ELDKRIIRDFEKNINKQFKNSLDQLLPQKLIPVVISRSGIDESKEVHQITKQERKDMV
                     KLLKGFTVTVSGTRPIEEAIVTSGGINLKEINPRTMESKLLKGLHFAGEIIDLDAFTG
                     GFNLQIAFSTGYAAGFFA"
     gene            complement(17674..18300)
                     /locus_tag="PKU88_10310"
     CDS             complement(17674..18300)
                     /locus_tag="PKU88_10310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPX69706.1"
                     /translation="MTRLYLVRHGETEWNKNGKVQGRTDTDLSPEGVIQAKLLARRLA
                     REDIDVIYSSSLKRALKTAEIIAEYKQCEIIESDKYHEICFGPWEGMTINEIKDKYGE
                     HFRVYKEDPVNFKLPGAETLIDLTERTYGAVMDIVSRHKGSNILLVSHGTAIKAVIIR
                     ILGLDIADFKKFMIDNASLSILEFPENSSGKPVVICLNDTGHLEEENL"
BASE COUNT         4863 a         4427 c         3006 g         6145 t
ORIGIN      
        1 cattattaac tttctctggt gcttttcttt ccgacatata tttacctcaa tatttattta
       61 attcgcatat aaagaagaat gcgacgcatc tgtagtttgc atagacgcgg ggacatgggg
      121 acgtttcccc tgtcccgctt tcagtgggac acgcgaaacg tccccgtgtc cccgtgttac
      181 ccgttaccta cgtcgcatta ttcttctatt gcgctattat atttttgttc atattataac
      241 atatatatac aaatttatca tgaactttaa ggcaaaagag gcaaatcgca atcttttacc
      301 cttgaggggt atgcaataac ctgctcgtaa ggcggaggca aaagatccgc attcctgctc
      361 tcagttatcg gaatatcaat tccgctttgg ttttcatcac tttgtctcat cagttcctct
      421 acaattattc ttcctatttg acggcccagc ctcaaacctt cggttatatc ctcaataaaa
      481 tgtactcctg cataaagcct ggatatgctg ttttcctccg ccagttcttt taatctttct
      541 gcttcgggag ggaagaaata gctgagtatg acttccgcag ttccggatat cactgagtgt
      601 ccggaaggat agctcggaaa cttcggagtg cataatgcgg taactaacct ttgatccaac
      661 tgattcgggc gcggcacatc ccataaatac ttgaaatacc aagtaatcac aaaggcatca
      721 ttaatgcctg cctgtacagc agccagcacc cttgctgcac gtaccggaga aaatccgtaa
      781 gtatccagca gccggtcgat aatcggcgtc cactgtttgg ttgcaggtcc gtccccccaa
      841 tattccgcga ttttgcgctg cttcggagtc aaatgcttaa gcacgccctt tacaattaac
      901 acttacactg ctttttcttt agtttcaggc agaatttcca tattaccatc ccttcaaaat
      961 ttaatgggtt aatctgccaa tgctcacacc caattgaatg attgtccatt ctcaattaca
     1021 gaatacgtaa ttttattaaa tttgtgcaat cagacaaaaa ataaaatagc caagtactat
     1081 ttttaataag cacccggctt ttttattgat ttataatact atcctttttt ctcactatac
     1141 ttgtttttcc ttcagagata tatttatatc aaattctcct atgggagttg taagaattaa
     1201 ctgtaaagcc ctttcaccgg ccattgtgaa tgatttgtat tccccgataa aaatctgcgg
     1261 agggacaata tcgcatgcgt agttgttttg tgccaaaagc gtcattgcat tgccgcttat
     1321 tatattagcc acttccccta aagcggagga tgcaaagctg tcaatcctgt ccatttccat
     1381 tccggacatt atccttatca tttccaaagt catatctttc gggaatccaa agaggatagt
     1441 gcccctcaaa tctccggtaa ctccaagtac aacacttgca tctttgctgc ttaccatatc
     1501 ttcaatgact ctaagatcct ttttctgagg atctatcccc accattagtt taaatacttc
     1561 cttagttgcc ttataaaagg agtttatata ttcagccttc attatgcttc tcctcccttg
     1621 cttttagcat actcccctat catctgatcc attttcccca catgcattat cagccaagcc
     1681 ataagctttc cgccgaattc ctgtatcttt tcttcgttga agccttcctc ctcaaagatt
     1741 cttacataat catttattcc ctctttaaat tttaaatgtg ctattttatg attctcaatt
     1801 tccgggtaac cgatctgttc ctggtaagct tcctcatcgt caaaatggac tattacgtac
     1861 tcctgcatga aggccattgt ttccttaacc ttttccagct tgtcctccca gttgtccttg
     1921 ctctcaactg tccggagaaa gtcagaaacc cgcttgaaaa gctctttatg ctgctcatct
     1981 ataagttcta tacctatact atacttgtct ttccacatca tagttaaaac cccccttttc
     2041 tttgtaatta tttttctttg attattatta tatataacat attatagcat aatttgccat
     2101 attgaacatt aaaattaagc gcaataaaaa tcaagcaaag aaaacctcgc ttgattctta
     2161 ataatatttc gcattaaatc attctttacg gctacttctt aacccccagg tatgcctcct
     2221 gtatttttgg atcgcttgct atatcacttg cctgtccgga agctgatatt ttcccctgtt
     2281 ccaatacata ggctctgtca gctatggaaa gcgccttata tgcattctgt tccaccagga
     2341 ggattgtttt tcccatcttt cttatatcca atattatgtc aaaaacagtc tgcaccaata
     2401 acggggcaag tccgagagac ggttcatcaa gcagcataat ctgcggactg ctcatgagtc
     2461 ctctgcccat agcaagcatt tgctgttctc ctccgcttag ggttcctgca acctgatttt
     2521 ccctttcctt aagcctcggg aaaagagaat ataccttctc caggtcctct ttaatctcat
     2581 ttttgtcatt tctgaggtat gcacccatat agagattgtc cttaacagta agattggcaa
     2641 atacaagcct gccttccggc acttggcaaa tacccgtttt gactatttta ctgggattaa
     2701 atcctaattt gttgtcgtta tagcttattt cgccttgttt atactttaag aaccccgata
     2761 tggcattgag cagggttgat tttcctgcgc cgttggaacc tatgatagat actatttctc
     2821 cttcattgac ctcgaggttt atccccttta atgcatgaat cccgccgtag tatacatgta
     2881 aatccttaac ttttaacata gctatacctc cacccccaaa tacgcttcaa ttaccgactt
     2941 gtcattctgc acatctttgg gagtaccctc tgcaagcttg cagccgaagt taagcaccat
     3001 aatattttca cagacaccca ttatgagatc catgtggtgt tctattatca gtatagtcag
     3061 attaaatttc tttcttattt ccgatataag cttcataagc tgttctgtct catcaggatt
     3121 catccccgct gccggttcat ccagcagaag cagtttgggc ataagcgcaa gagcacgtgc
     3181 tatttcgagc cttctttgca gcccgtaagg caaattgttg gcaatcaggt cttttctgtc
     3241 ttcgaggccc attattgcaa taagctcagc ggcttgttca ttcagctttt tttcctgcat
     3301 tctgaacttc ggcgttctca gcaccgaatc caaaagtgaa tagtttgcgt tataatggca
     3361 tgccgtaagt acattctcat aaacagtcag ttttttaaac agccttatgt tttgaaaggt
     3421 tctggtgatt cctgattctg ctataaggta cggctcctta ttagtaatat ccttgtcgtc
     3481 gaatattatt ttcccgcttg taaccttgta aacacctgtt acgaggttga atattgtggt
     3541 cttgcctgct ccgttaggcc ctataagccc tgtgatgctg tccttttcaa cattgaaatc
     3601 cacgtccccg actgcggtaa gtcccccgaa tgtcttggta aggccgtgca attcgagtat
     3661 gcccatatta cttgccacct cctgcacgct gaagcttatt ctcacgcttt gccgccatat
     3721 ttctaaaaag ctttttcata ctggaggggg tcagctcgta gccgcccatc aagccttcag
     3781 gccgcgatat catgatgaat ataaccgcca atccgtagaa caccagcctc catgaagaaa
     3841 aagcccttaa tatctccgga agcgaggtaa gcactaaggc acccagcaca ctgccggata
     3901 tgctgcctaa tccgccgaat attacaatga tagtaagctc tgtcgatttt gcaaggctga
     3961 acatcttagg ctgcaggaag gtcatgtaat gccccatcag ccctcctgct attcctgcat
     4021 aagctgcact tgtaaccaga gaagtcatct tataattaaa aacattaata ccaacaatct
     4081 gtgaagccaa ctcctcctcc ctgactgcga tcatgttccg tccatgccgt gagcgtatca
     4141 aattggcaag tacaattact gctacaatat taattccaag tacattccag acatttgtat
     4201 aatggggtat ccccggccag cctcttgcac cgccgaaggt ctgtacatta tccagtatca
     4261 gccttattgc ctctccaaaa cccaaagtag caatacaaaa gtagtcaccc ttaagattca
     4321 gggtcacttt acccagcgcc gcactgacca atgcagccgc aagcccgcct gccaaaagtg
     4381 ccggtatgaa gggccaatga aattttaccg tcatcagtgc cgtagcatag cctcctattg
     4441 ccataaaacc tgcatgtcca aaggagaaca agccagtaaa tcctgtgagc agcgataacc
     4501 cgagtaccgt cataaggttt attccggcta atgtgagtat acccaatatg tattcatttc
     4561 ccatatctat gcccccttat gctttatcct cggtaacctt gcccatgagt cccataggcc
     4621 tgactaccag aacagtaatt aatagtacaa atgcaataag atctctatac tgagatgaaa
     4681 ggtaccctgc tgtaaaggtc tcaataatcc ccagcagcac agaacctatt acagcacccg
     4741 gcaggcttcc caagcctcca aataccgcag caataaatga tttatttgta ataatgccta
     4801 tttggggata tacggtatat ttcataccga acagtactcc ggctataccg gctaaaaaac
     4861 cacccataag gaatactgta attaatataa agtcagtatt gacccccatt aaggcactgg
     4921 ctttcaagtt gtaggaagta gcctgaatag cttttcctat ttttgtatga tcaatgatat
     4981 aaatcagtat tatcaggcat atggcggata tcaggaacat tgctatatca agctttccca
     5041 ttgccaattc accaattttt atcggctgtt tattaaatac ctcaggatat gtcctaaagg
     5101 tcggtccaat ggcaggaata gctattacaa gattctccat aaaaatagat gcacccatcg
     5161 ctgatattat aaaatacaga aatggcgctc ttctttctct aagaggctta tatgccactc
     5221 tctcaataat cagtgctaaa atggcagagc atatggcagc cgaaatgaat gccaaaggaa
     5281 aaggaaacct taaaaatgta agaaaaaaga atccgaaata tgccccgaac ataattacac
     5341 cgccgtgggc aaaattactg aaattcataa tactatatac gagagaatag cctacagcca
     5401 tcaaagcata tacacttcct atggaaagac cgtttataag ctgttgaaag aattgtgtca
     5461 ttattttctt ctcctttaca cgtttaggga aaataaactg tattgagcag aaagaacatt
     5521 tatagagcag aaggagtttc cttctgctct atttcatatg gtcaattaaa gcctattctg
     5581 cagcaaactt ctgctggaat acatacttgc catcaacaat ttttagtata gcagcatctt
     5641 taccttccgg attatgggta tccttgctta tctttattct tcctgtgata ccctttatat
     5701 ctgtagtttc aagagcttga gcaatctttt cgccattggc ttctcccgct ctggtcaaag
     5761 catctacaaa catgagaact gcatcgtgta caagataacc gttaagctca acgtcttttt
     5821 cgtactttgc cttgtatctg gtcttgaagt cctgaacttc aggatcatca taatccaaat
     5881 gatttacgaa gaaggaacct tctacggcat ccttcgccat gctgataagc tgctcggacg
     5941 gccatccgtc tccacccatg agtgttgcat ttatgccaag ctctcttgcc tgattggagc
     6001 ttaatgccac ttccttaaag aagtaaggca tgaatataac ctcaggattt gctgccttaa
     6061 tctttgacag ctgcggcctg aagtctacgt cacctgtctt gaagccttcc ttggcaacaa
     6121 tggttccgcc tttttttacg aagaactctt caaagaactc tgtaagtccc tgtgagtaat
     6181 catccccaac atcataaagg actgcagcag tcttggcttt aagaacatca gccgcataac
     6241 ctgcagcaac ggcaccctga taggggtcaa taaagcatac tctgaagtta tatggcttta
     6301 ttttaccgtc aagtacagta acctttggat ttgttgcaac tgtagcaata tccggaacct
     6361 ttgcctcttc aaggacaggg gctatcggta tagcattccc gctggcattt gggcccagta
     6421 ttgtgcaaac cttgtcctga gttgtaagtc ttttagcaac atttacagct tcctgcgggt
     6481 cgccttttgt gtcataaccg ataacttcca attgctttcc aagtactccg ccttttgcat
     6541 tgatttcttc aaaaagcatc ttgacagtat tcagttcaca ctgtccccat actgcctggt
     6601 cacctgtaag agaacccatc cagcctacta atattttttc ttcagcagga gctgcgggtt
     6661 ccgctggtgc tgctgtctgt gggctgcagc ctgcaagtcc ggctgaaaca gccatcaaac
     6721 ataccaatag taatgccagt actttctttt tcataatctt cctcctccaa attactgata
     6781 attaatttag aaccatatcc tcctttctta ataacgcatc agcaggaaat ttttcaattg
     6841 cagaaaaatt tactcgcctc tgcgcatttc aacgaggcgt gaaaaatgcc caaacctgtc
     6901 ggagacatct ttttgcctcg ctataataca aatatgaata ttattgtata aatatattta
     6961 aaaaaattag aaaaatgaca tgtatgctgc cggtaaaatt ttatcatcgg aataattatt
     7021 ttattctagc gtatgtctta agattctacg taaattaatt ttatccttct aaaaaaagcc
     7081 ttttaaataa aaaatacact atatggctat agtgtatttt tgccgcaaat gatgattaca
     7141 tcagctttgt cccctgattt tcaacaacat cccgttcctt aagcatcctg ataatcttgc
     7201 ttgcatcact ttttgaaaga tctttgctga agctctttcc cgtcttatcc tgtatcaaat
     7261 ctctcagttc atcatttgat atattaagat tttttgctat cgcaaatata gtattgattt
     7321 gtgtttttgt agcgccttca taatttttgt gttcctcggc ctgctggctc tgattctctt
     7381 cacttatgct tttcccttcc cccacctcct caaatcctgt atattcaact ccgtagccgg
     7441 caaatcgcaa tgccctggca attgctcttg tctctgccag ctcaagaatt gcattggaaa
     7501 gcaccttatc cctctttact gacgcattcc ccaatccgtt aaaggttcct ttcccggtct
     7561 tcacggcagc ctggaccact gttgattcct cattatactg cactacgctg gtttctatgg
     7621 ataaattctc gttctcatca tgggcaagtc tcaatctgcc gccaaccacg ggatagattt
     7681 ttccctgcac ctttacaagt tcttcattcc taaatgtact cggcatatta ttccctcctg
     7741 aatatatatt gtgagggtaa gaaaataata tgcatatatc ttcagcttat ttcctttttc
     7801 agttctgcaa tgctttcctc aagctttttc agatgataca gcaatgaatc attatttgta
     7861 ttgctgcctt cagaaataat gtattcaaga ctgtcaaaag tatcacccga tgcagccttt
     7921 agtgccctta aatagcttat gagattatgt ttattcatgc ttcttctttc aactgttatt
     7981 tccctggcag ctgatgaaga tagctctaaa gtctccccaa gctttgcggc ttcaacggga
     8041 atccccagct ctgattccat aagcttagac agcaccttcg acgattcttc ttctccatgc
     8101 accagaaata ctttttttgg cttttttata aaagacccta cccattctat aagccctctc
     8161 tggtccgcat ggcttgagaa cccctcaata gaatagacgt tacaattcac actgatttct
     8221 tcaccgaata tcttaacaag cttttctccg tcctttattc ttcgtccgag agtatttttt
     8281 gcctgatagc ccacaaatac cacacttgat tcttcgcgcc atagattatg cttgagatgg
     8341 tgctttattc ttccggcttc acacataccg cttgctgaaa ttataatact gcttccctcc
     8401 attgaattga tagccttgga ttcctcagcc gtgtgggtga atatgagatt aggaaaatca
     8461 aagggattat ctccttcttt aagcaatttc tgagtatcct catccatata tggcagaaag
     8521 ctttgaaata tgtttgttgc attaattgcc agaggactgt ctacaaatac cggtacattg
     8581 cataatggta atgatttgca ttccttcagc atattaagta tatacagtat ttcctgagtt
     8641 ctttcaactg caaaggaagg tattaccacg ttgccgttcc tctctattgt atctgttata
     8701 atatccagca gggccagagt cttgttattc atctccatgt gaagcctgtc cccatatgtg
     8761 gattccataa ttatataatc tgcttcttca attatcgtcg ggtcattcat aatgggtgca
     8821 tctttatttc ctatatctcc tgagtatacc agcttaatct cttcgctgcc ttccctgatc
     8881 cataattcta taattcccga ccccagaata tgtcccgcat ccattatcct gaatgaaatg
     8941 tccgggtcaa tatttataat cttattgtac tccacaggtt gaaagaattt taatgatgct
     9001 tctgcatcct ttactgtata taaaggctct gcaactcctt ttcctgctct ttttcttttc
     9061 ctgcttttcc attccgcttc tgattcctgt atgtatgcac tgtcaggaag cattattttg
     9121 catagttcaa aagtcggggc agtagaaatg atcttaccct tgaagccctg tttgacaagc
     9181 agcggtatcc ttccgctgtg gtcgatatgg gcatgggaaa gtataagcat atcgatttct
     9241 gaaggattga acctgaatgg ttcaaaattc atttcctcag cttcacttcc gccttgaaac
     9301 atcccggtgt ccagaagtat ctttttattt cccgtctcta taagatgaca ggaaccggtt
     9361 acagtacctg ccgcgccgca aaaagtaagc ctcatattac acctccgtaa ttactcaaat
     9421 tatacacata tattgactat attctacatt gattcnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnacctc cgtaattact caaattatac acatatattg actatattct
     9601 acattgattc accattacct ttaaaaaacg cgtctcacga cgcctttttt ttgctatgga
     9661 accctatggt tcccgaaggc ttcagttccg cagctgagca ccctccttca acggcagggg
     9721 gcttctcccc cttgcatccc ccattgtaca aggaaattat actttccttg tacaattaaa
     9781 aaacaaaaag accttaagaa aaataatctt caaattctta ttcttgaaac attggaatca
     9841 aattctattg acaataaatt catttcgtta tatcatttat attgtcagtt tgcggggcgt
     9901 agcgcagctt ggtagcgcgc ttggttcggg accaagaggt cgcaggttca aatcctgtcg
     9961 ccccgaccat tgaaactaca gctcaataat atccgggtca taatccaaat ccaatccata
    10021 aaaattgata tagcaataaa tgaactctct tttatactct ctgttcctta actgcctttt
    10081 cagctttttc aaatacttct tttcgttcca ctttttattt gctatatacc gttttctgcc
    10141 cagcttccaa aacttgtgag ggaaaaccaa tattgccagc agcatttcat attcttcctt
    10201 tgacatgggc cgaagcttgc aatagctcgc aataagcctc ctgcacaagt caaaatccca
    10261 tttgaacctt ttcttcgaga gtatccttct tatcatttta cctaaatcgc tcatgggaat
    10321 atcgtattgg catgactcca ggtcaactac aaacagttta ttgtttctgt caacaattat
    10381 gttttgataa tagaagctgt cgtggcatat actgccttca tttatataat attcgcaaag
    10441 ctcattgtac ctgctgtggt caagtattct gattgcaagc tcggcttccc ttataaaata
    10501 gtcaatattg ctcttatata tgtaatcgaa ctcggctttc agcttaagct tatcgatata
    10561 ttccctgtat cctgccagtt catcccggca ctttgcgaag gttcggcgcc actttttaat
    10621 atgggactta accttcacat gcttaggcgt cctgaatcct tttgccctgt tgtgaaaatc
    10681 cgccagaagc tcggaatatc tttccatttc atccggtatc gaaaagcttg cctcgcaacc
    10741 ttcgatccaa taagtaaggt aaaaagccgt gcccttgcgt tttattattg gctttccttc
    10801 ttttgtgaag taatattccg caatgtagtc acatccgttt tcttttaaat gcagcataat
    10861 attatagctc tttttggctt tttcataacc attgtttacc cgtttcaagc agaaaaaacc
    10921 tttatccgtt cctaccttat atgcacttct gactttatct atcgattcta cccgaaaatc
    10981 ataatggttt aatacaacag caagggttct catttctttt tggtctatat ccgatgtaca
    11041 tttgctttcc agctgtgaca gcattatatc cactccaatc acaatatgta cctataattc
    11101 cggttaatat attatatttt ggagtgctat gatatatatc aaaagaaaaa acaaacttgc
    11161 cgattggcaa gtttttaatg accgctgtta cacttcctat ggttgtccgc tttttttgta
    11221 gaagaaagtt tttattgact ccagtttata tttatatgga agtattatct gctccgtgct
    11281 gccgacaaac aacactccgt tgttgcttaa ggaattcctg aaattatggt acattgttat
    11341 cttagcttct tccgtaaaat aaatcattac atttctgcat acaatcagat cacagttttc
    11401 agggtatttg tccaccagca gattatgatg cctgaaatct atacagttga ttatcctttc
    11461 acttaccttg taataatctc ccgattttac aaaaaaacta tcaacaaaat ttcttggcag
    11521 gttctcaagg ctcttcgcgg aatatatccc caccattgcc ttattcagcg cagcatcgtc
    11581 tatatctgtt gcaagtatcc tgaagctgtt cagatcaacg tatctgctca tcatcattgc
    11641 aagagagtag ggctcttctc ctgtagaaca tgcagcgctc catattttag gctttttatt
    11701 aaatttcatt atttcgggca ttatttcgtt ctccaggacc ttccactgct ccggattcct
    11761 gtaaaattca gatacattaa ttgtaagata attgataaat tcatcgtata gatttgaatt
    11821 ctgtgtcaat gctttaaaat actcatcgta atcatcatag ttgttcctgt ggataaggga
    11881 gtctatcctc cttcgcatct gccgctcctt gtagcttgac aaatctattc ccgtaagctt
    11941 taatatcttt tgtttaaacc cttcatagtt ttctagcata agcaactcct atcagattaa
    12001 taataagcta ttactattct atcatatgaa aaacataaaa aaaagtcgtg atatgtttta
    12061 tatcacgata atataatgta aagggggtct caatgtattt tgtgaggtcg atattagtgt
    12121 tgacaatata aaaatattta tacataaatg ttaaaacaaa ttaaaacagc acacttcttg
    12181 tgaatatcac aatagtgtgc ttgtattatg tttattcttc ggcagtttct tctgcggttt
    12241 cttcagttat gttttccgct gctgcttctt cagcttcctc agctattggc ttttctgctg
    12301 cttcctgctt tacttcttca ataactgact tctcagcttc ttctttagct tcctcaacag
    12361 ccggtttttc ggcttcttcc ttaacttctt caactattgg cttttctgct gcttcttcct
    12421 tagcttcctc aataaccggc ttttctgctg cttcttcctt agcttcctca ataaccggct
    12481 tttcagcttc ttctttagcc tcttctccaa ttgcttctat tagttctctt atactcaagc
    12541 ttattttctt ttcttcaggc tttatatcaa ctatttttgc tttgacaatt tctcctattt
    12601 tcagagcatc tgaaggcttt gcaattcttt tgtcagatat ctgtgaaatg tgaataagac
    12661 cgtctatacc cggctcaagc tctacaaagg ctccgaagtt cgaaaacctc aatactttaa
    12721 catcaagaac atcgcccggc ttatactttt ctcccgcagc agaccagggc tccggaacag
    12781 tctgcttaag cccaagggaa atctttttct tttccttgtc aacggataat actattactt
    12841 ccaccttatc gccgggtttt acaatctcag aaggatgttt gactcttgac caggaaagtt
    12901 cagatatatg aatgagtccg tctactccgc caatatccgc aaaggcgcca aaatcagcaa
    12961 ggcgcttaat ctcaccggtt aatctctgtc cgacttcgat gttttcccat aacgcttttt
    13021 ctttaatttc attttgcttt ttcaaaagcg actttcttga tacaacaact tttcttttct
    13081 gcggatcaaa ttcaataatc tcaacattaa ctttcttatt tacaaaactg ctaagatcct
    13141 ctgaataccg cacatcaaac tgggatgcgg gcataaacgc cctgacgccc ataagttcga
    13201 ctattcctcc gcctttaact atttccttta caaaaccctc cagaggggtt ttttcattaa
    13261 atgccgcttc tattttatta aagttctttt cggcatccag aagcttcttg gaaagcacga
    13321 cattaccttc accgtcattt attcttacca ccattacttc caattcgtct ccggctttta
    13381 ttatatcctt caggctaaca tcagtatccc aggtatagtc ttccttcttt attattccgt
    13441 cggatttata tccaatgtta accatgacct catcatcggt aaccatgatt actgttcctt
    13501 ttactgtgtc tcctggatat accggcctca tggtcttttc atattcttcc atcatatttc
    13561 cctggtcttc tgtcgtatct tcctgcttct ccatttcttc cccatcatcc actacgcttt
    13621 cctgattctc cattatttct accggatccc ccataatatt gccgttgtcc tgcatttttt
    13681 cgataacctc cttaattaac caatcaggtg tggatgcacc cgccgtaata ccaaccgtct
    13741 caaatccctt cagcattcct gccggcaaat cctccgcttt ctcaatatga taagcattat
    13801 cgcaattctc caggcatatc tgataaagct tattagtatt agaactcttt ctgcttccaa
    13861 ttacaatcat acaattaact ttttttgaaa gctgttcagc cgattcctgc cttttctcag
    13921 ttgcgctgca aatggtatta aatattattg catttttata tttcttatta atagcatcca
    13981 ctacgctgtc ccagattttt tttgtaattg tagtttgtgc aactaaacat attttatcat
    14041 atataggaag tttgtctact gttttttcat cctcaattac atgggcggaa ttgtcacacc
    14101 acccatttat gcctattact tcgggatgag ctttatctcc cgcaataaat attttatagc
    14161 ccttcttata atattcctgc acctttttat gtattgccga tacataagga caggtgcagt
    14221 caatatatcc gaatcccttt tcttcaattt gcctgtactc ttcctcacct attccgtgtg
    14281 aacgaataat aagctcggca ccttcacaat ttatctcatc aatgctgtcc gcagatttta
    14341 tacccaattc ttcaagcttg cctgtcacct gatcattatg aataaggggg ccaaatgtaa
    14401 aaatccttct gtccttcttt ttatccgctt cttcaaaggc tttatccaca gctcttttta
    14461 caccgaaaca gaatcccgca tatttatcaa taattatttt cattttccct atccttcttt
    14521 agctcattaa ttcttttcat gatatcgaca cttattttaa tatactcgtc attcaaaagc
    14581 ttcttatcat agaattctgc aagctcaata ggctccccta taattatatt tgtccttttg
    14641 aaaaacttat agctgctgat aattgccacc ggtacaatcg gaacttttga ctttattgca
    14701 agcattgcaa taccgggctc agcagtaatt ccatcacttt tcttccttgt tccttccgga
    14761 aaaagagcaa gaatttttcc gctgttcaac agtttaagag aggtttttat gctcttaata
    14821 tccgcttccc ctctttttat cggaaatgcg cccagggcgt tcatcaatgc cctaagcagt
    14881 ctatttctga ataattcaac ctttgccatg aagtacatcc tgtttggtat cgaaattccc
    14941 acaagtatcg gatctactgc acttacatgg tttccacaga ctatatatgg gccgtccttt
    15001 ggaatgtttt cacggccggt tattctgtaa ttataaaata ataaaaatac cggtatcagt
    15061 aatgacctgg caaacctata aaacatcagc gcctcccccc tttgattaaa tatatgaaac
    15121 tacttcattc aaaacctcat ctataccttt acccgtcgta tcgattatta tggcatcaga
    15181 ggctgctttc agcggagaat cacttcttgt agaatcaata taatcccttt tttctatctg
    15241 tgacctgata tcctgaatct cagcttcata gcctttctgc ttcagctctt tatatcttct
    15301 gtcagctctt tcctcaacag aagctgtaat ataaaacttt acatcggcct ccggaaaaat
    15361 gactgtgcct acatcacgtc catccattac cacattcttg ttcatggata tcttcctctg
    15421 aatatcgact aaaagcttcc ttatctcaga tattgccgca atatatgata catttctgtt
    15481 tacatcctct tctcttattt ctgtattgac agctctgccg tcaagatata tgctgttatc
    15541 ccgaaaatct atatcagttt tttcagccat gctgatcagc atatctgttt ttttcaaagg
    15601 aatactctct ctcagcacct tcaacgttat tgccctgtac attgccccag aatctatgta
    15661 agtatagcct atcatatctg caagcttttt tgctatggtg ctcttgcccg ctccggcagg
    15721 cccgtctatg gctattactt tataaccgct catggataat ccccccaata ctttgccatt
    15781 tcaattacgc cttcactgat gataccattt taccagaaag gatttcaaaa taaaagatta
    15841 ttggtcacag atacgattct actttattct tatcctatct accatcacaa gattatcttt
    15901 cacatataca agctttcccg gttcgggcac cgttatgctg tcggatacag ccgtaacctt
    15961 aagaactgcc gtatcattca aggggccgcc tcctacctca ataatatcac tgtcctttac
    16021 tttcagcctt attgatcttc cgttcggagt gtcaatgctt tttccgttta tataaaaaac
    16081 cagcggcttt agggttgaat aattctctat ggatatctca agctctcccc ttttaataaa
    16141 aagctgactg tcggctatgg acctgccttc cagcttgtct atcctgctta taatgttttt
    16201 tattgtctgg cttaacagca atgcttgtga aattatgaga gctgcaaata aaagaagcag
    16261 aacttttctg attaaaccgt caatcctgtc ccaccagctt tggaaactgc tgttcactat
    16321 ctttatcctc cctcatatgt atttatttag ataattcctc tttcgggagg catttattca
    16381 gttatgcaaa aaaacccgcc gcatagccgg tggaaaatgc aatctgcaga ttgaagcctc
    16441 cggtaaaagc atccagatca attatttcac ctgcaaaatg caatccttta agaagctttg
    16501 actccattgt cctggggtta atctccttca gattgatacc tcctgaagtg actatagctt
    16561 cttctatagg ccttgtgcct gatacggtga ctgtaaaccc cttaagcagc tttaccatgt
    16621 ccttcctttc ctgcttggtt atctggtgaa cctcttttga ctcgtctatt cccgatcggg
    16681 atattacaac cggtatgagt ttttgcggca gcaattggtc cagtgagttt ttgaactgtt
    16741 tgtttatatt tttttcaaaa tcccggatta ttcttttgtc aagctcctcc tcggaaagag
    16801 ccggtttcag gtcaatgcta atcctaattt cctgcttcct tctgagataa tccgaaatga
    16861 aaaagctggc agacaaaatt attgggcctg acaatccata gtgggtaaaa agcatttctc
    16921 cgaaatcctc atataaaagc ttcccaccgg cgtaagcttt aattgcaaca tttttcaggg
    16981 acagtccctg cagttcctta acaaatgcat catctgacac cagcggcacc aatgacggcc
    17041 tgggctctat tatgctgtgt cctgccttta cggcaatttt atatccgtct cccgttgacc
    17101 ctgtattagg atacgatagt cctcccgtgc aaatgattat agagctgcat tccacaaccc
    17161 ggccgttgtc aaataaagcg cctgttacag caccgttttc ctgtcttatg tccaggagct
    17221 tactgttgag ccttacatca accttattcc tattcaaata tctttccagg gcttttataa
    17281 catcattgga cttgtcggat tcggggaaaa ccctcccgcc tctctctacc tttgttttaa
    17341 gtcccaggga ttccaaaaac aaaataagat cattgtttga aaagctgctg aaggagctga
    17401 agaagaattt tgaattcttc ggaatattct tcatgaattc atcaaaatcc gcggtatttg
    17461 taacattaca tcttcctttg cccgtgataa acagcttttt gcccagcttc tcattctttt
    17521 ccgccagcat agtccttttt ccttttgagg cagccgtacc ggcagcaatc attcctgcgg
    17581 ggcctcctcc gacaactatt acatcatata tcaagaaaaa catcctttca accatattat
    17641 ctcaaacgca gaattataat atgtttaatt ctgctataga ttttcttcct ccaagtgacc
    17701 ggtatcattc aggcatatta ctacaggctt tcctgagcta ttctcaggga attccagtat
    17761 tgatagtgat gcgttgtcta tcatgaattt tttaaagtca gcaatgtcta atcccagtat
    17821 tctgattatc actgctttaa tagctgtgcc atgggatacc agcagtatat tgctgccttt
    17881 atgacggctg actatatcca taaccgcgcc atatgtacgc tctgtcagat ctatgagggt
    17941 ttctgccccg ggcagcttga aattaacggg atcctcttta tataccctga aatgttcacc
    18001 gtatttgtcc ttgatttcat ttattgtcat gccctcccaa gggccgaaac atatctcgtg
    18061 atacttatcg gattcaatga tttcgcattg cttatattct gcaataattt ccgcggtttt
    18121 caaagctctc ttcaggctgc tggaatatat cacgtcaata tcttcccttg ccagccttct
    18181 tgcaagcagc ttcgcctgta ttacaccttc ggggctgaga tctgtgtctg ttcttccctg
    18241 aaccttgccg tttttattcc attcagtttc tccatgtctt acaagataaa gcctggtcat
    18301 caaatccctc cttaaatagc catattaatt atatcataga ataataataa tgcgacgggg
    18361 acacggggac gtttcgcgtg tcccactgga agtgggacag gggaaacgtc cccatgtccc
    18421 cgtcgcatta ttattcgatt gcgaatcaaa tatgaatgaa aaaatcctgc tttgtacaaa
    18481 gcaggattca gactgaagac aaagtataaa attttcgaga gaatccttga aaacaaaaag
    18541 g
//
LOCUS       DAOOAB010000053        17214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_4314_length_17214_cov_15.694621, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000053 DAOOAB010000000
VERSION     DAOOAB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4314_length_17214_cov_15.694621"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..1546)
                     /locus_tag="PKU88_10315"
     CDS             complement(356..1546)
                     /locus_tag="PKU88_10315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPX69707.1"
                     /translation="MNRLVFLVDMNAFFISCEMAMNSELKGKPAAVAGDPKKRSGIIL
                     AANYEARKYGIKTTMVLHEAKKLCPELVTVPPSHGLYEKKSKEVMDILSRYTPVIQQN
                     SIDEAWMDLTGCEKLFGSPMEIAENIMKTISEELDLWCSIGISENKFLAKMGSELKKP
                     QGITELYVKDIEKKLWPLPVREMYGIGKQTEQKLMDYAIYKIGDIANSNVEFLKKNFG
                     KYGEELYRLSRGIDNSPVVPNPIYDNKSISRSTTLPQDTNDLEYLKYILLGLAEEVGY
                     EARKSGYKGKTVSISIKYEDFKTVTRQKTVAPTYLTKDIYTNALALLETNKNKNKLIR
                     LIGLGLSNLDTDETEQLSIFELVEGEHNAHKEETIEKTIDRIRERFGDNKILRGSMLK
                     NKKG"
     gene            complement(1642..2268)
                     /gene="yunB"
                     /locus_tag="PKU88_10320"
     CDS             complement(1642..2268)
                     /gene="yunB"
                     /locus_tag="PKU88_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HPX69708.1"
                     /translation="MRRKFGQRGGRKKLFLSFILLIIISIYSYYIVERNIRPTILAMS
                     EINARLIATQAINEAVRSKVITDSFSKLIDYKTDNNGRISLIQANSAQMTKLAAETSI
                     EILNEIKKIGITDLKIPMSSIFGSQLFANTGPVIGVNIRPAGSVNVDFFTDFEEAGIN
                     QTRLKIYLTVKTDVQIVVPLGSNKIDVTTHIPVSETIIVSEVSEGKTA"
     gene            2537..4900
                     /locus_tag="PKU88_10325"
     CDS             2537..4900
                     /locus_tag="PKU88_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPX69709.1"
                     /translation="MSENETNSPKKKKKKKRTFSVGRFLRFIILLLLFIILVSGSIAL
                     GTAYSWVKAARPLNVDELFDLNQTTYIVDKNDKLIDKLHANENRTIATLDQIPEYLQN
                     AFIAIEDKRFREHRGIDLYRIFGALKADLASGEFSQGASTITQQLIKNVYLTPDKKWK
                     RKVVEMYYALQLERRFLKDQILEAYLNTIALGHNVAGVKEAALYYFGKELDQLTLAEC
                     ATIAGITQYPSLYSPYLNFEKSMGKKEIVLEEMLVQGLISQEEYDEAMKQEIKLSKVE
                     KEVETTYFADMIISDVTETLQKELGLSKEEAQIKLFNGGLKIIATIDTEIQNIVEETF
                     KNTKLFPESEEDANGILQPEAAVIIIENGTGEIKAVMGGRSEKVRRGLNRATQSLRQP
                     GSTIKPLAVYTPALDNGYTAGTVIDDAPVIFGNFKPRNYSNNFKGLVTAREALQNSTN
                     VVAVKILQDVGIQRSIEYLKKFGITTIVTREDNGVTNDESLSPLALGGVTNGVKPIEM
                     AAAFSVFPNKGIYIKPISFTKILDKNGNVIYENKPYKDKVISEQVAYLMVDLMRGVVR
                     GGTGGNAALSKMPVAGKTGTTTKNVDAWFTGYSPYYTASVWIGHDEPKPMNFTGGSYP
                     AKLWKAVMEEIHKNLEVKNFEKPGGLVSVSICTVSGKRPSELCALDPRGTRVRSELFI
                     KGTEPDAETICDVHVIRDACTDSKKLATEFCPAPSVQSQVFIQRIEPFIPEPESDQKQ
                     LPADLIYEAPIEPCDIHTEPTFEEDPLEGEIDLEGEIDLEGEEDEEF"
     gene            complement(4945..5517)
                     /locus_tag="PKU88_10330"
     CDS             complement(4945..5517)
                     /locus_tag="PKU88_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HPX69710.1"
                     /translation="MNETKKRVILVTDGDGIARKAVETAATRIGGRCISKSAGNPTPL
                     SGQKIIELVKQAPYDPVVVMVDDRGHTGTGKGEKAMLEIARSPDIEVLGVIAVASNTE
                     GVKGVSIDFSVTKNGNIVEKTVDKNGNITAGGKINGDTVDILDSIDVPVIAGIGDPGK
                     MDGKDCCDKGAPIITKAMKEIIERSRVHTH"
     gene            complement(5529..5885)
                     /gene="spoVAE"
                     /locus_tag="PKU88_10335"
     CDS             complement(5529..5885)
                     /gene="spoVAE"
                     /locus_tag="PKU88_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HPX69711.1"
                     /translation="MDYLKAFLVGGAICVIGQLLMDLTKLTPARVLVAFVTAGAILQG
                     IGIYQAIVDFGGAGATTPLTGFGYSLAKGAMKEVKEAGLLGAFTGGLKSTAGGIAGAI
                     FFGYIMTLLFSPKSKK"
     gene            complement(5885..6913)
                     /gene="spoVAD"
                     /locus_tag="PKU88_10340"
     CDS             complement(5885..6913)
                     /gene="spoVAD"
                     /locus_tag="PKU88_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HPX69712.1"
                     /translation="MAGKRLGKQTLKLSSPPSVISNAAIVGPKEGEGPLSQYFDQILT
                     DDYFGEDSWEKAESKMLKEAVKSAVKASGRSNTNIDFMFAGDLLNQIVSSSFAARELQ
                     IPYIGLYGACSTMAESLCMGSLMIDGGFAEAIVCATSSHFCSAERQYRFPLEQGVQRP
                     PFAQWTVTGAGAAVIAETGSGPYITHATIGKVQDFGIKDANNMGAAMAPAAVDTITAH
                     LNETGRRPEYYDLIITGDLGNIGKAIILDMMQKEGYDITKVYNDCGSMIFDSEKQDTH
                     AGGSGCGCAAVVLNGYIAKEMKKGNINKVLLIATGALLSPTTTWQGESIPCIAHAVAI
                     EKTIGVGV"
     gene            complement(6945..7379)
                     /gene="spoVAC"
                     /locus_tag="PKU88_10345"
     CDS             complement(6945..7379)
                     /gene="spoVAC"
                     /locus_tag="PKU88_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HPX69713.1"
                     /translation="MSSKQYEQYIKDKLPKPAYLKNCIAAFIVGGFICDVAQFISNTA
                     KSYGYNQEAVSSITAASMVFLGAFLTGIGVYDRIGKFAGAGSIVPITGFANSIVSPAM
                     EFKREGFVFGVGAKMFILAGPVLVYGISSSILIGIIYYLLGK"
     gene            complement(7419..7553)
                     /locus_tag="PKU88_10350"
     CDS             complement(7419..7553)
                     /locus_tag="PKU88_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69714.1"
                     /translation="MFSNRRFKQLKNILGFESETEKEIRSDIGGKIIKNSARVFEHRQ
                     "
     gene            complement(7543..7695)
                     /locus_tag="PKU88_10355"
     CDS             complement(7543..7695)
                     /locus_tag="PKU88_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69715.1"
                     /translation="MKIFKLFLVLSVIIAVCYFSWDYYAGKLLKENRERATLVFGEQG
                     SGYYVQ"
     gene            complement(7898..8632)
                     /gene="sigF"
                     /locus_tag="PKU88_10360"
     CDS             complement(7898..8632)
                     /gene="sigF"
                     /locus_tag="PKU88_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HPX69716.1"
                     /translation="MERVELTQDEFLELISRAQRGDSEAENFLVENNIGLVRSVVRRY
                     LNRGCEYDDLIQIGSIGLLKAIKKFDLSYNVKFSTYAIPMIIGEIKRFLRDDGIIKVS
                     RNLKEIANKAHIAKEALEKDYGREPTISEIASVLEISKEDLVVALESVQSTEYLYETI
                     HQDDGSPILLIDRISFNEGEEIEVIDRLALKEVLSALEPKERQIIMLRYFKDMTQCQV
                     AQMLGMSQVQVSRVEKKIISKLRQNM"
     gene            complement(8642..9073)
                     /gene="spoIIAB"
                     /locus_tag="PKU88_10365"
     CDS             complement(8642..9073)
                     /gene="spoIIAB"
                     /locus_tag="PKU88_10365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HPX69717.1"
                     /translation="MKADNEMKLEFLSKSQNESFARSVVAAFVAQLDPTIEELAEIKT
                     AVSEAVTNAIIHGYSCEIGNVVIVSRIFGDTVEIEVKDFGVGIADIELARQPLYTSKP
                     ELERSGMGFTVMETFMDKVEIESLPWKGTTIRMTKKITTVR"
     gene            complement(9081..9422)
                     /gene="spoIIAA"
                     /locus_tag="PKU88_10370"
     CDS             complement(9081..9422)
                     /gene="spoIIAA"
                     /locus_tag="PKU88_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HPX69718.1"
                     /translation="MSIDFSYADRLLVVRINGDIDHHSCEEIRSKIDKEIASKNPRSI
                     LFDMEQVGFMDSSGIGVLIGRYKLIANGGGKAGMINVKPQIMRLCEICGLQKIIKIYS
                     SKKQAIASIKE"
     gene            9676..10797
                     /locus_tag="PKU88_10375"
     CDS             9676..10797
                     /locus_tag="PKU88_10375"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX69719.1"
                     /translation="MNVLIFSVSIGNGHDQVAHTLRNEFIRQDNSSRVRIINTINYIS
                     PLLDKVILDSYLNILKFYPKAWGKIYEKTNRLDPIIDINDITNRLMTSKLRKTTINFD
                     PDITICTHSFPAAIISKLKQKKRIGCPLVTVVTDFNIHSSYINDNTDYYVIAHEYLTY
                     IMERFGVQKEKVLPFGIPIRKEFSESYNREELIAKLGYEDKKTILVMGGGLGLGDINN
                     IVKAIDSNMEDIQIIAVAGRNNRLESKLKDLSAKNRLVVYGFVNNMHELMELSDCIIS
                     KPGGVSVSEILSREKPLVIFSPLPGQEHENVEFLLNSGAAVYTSDVRRIPALINQIFD
                     SEIRMKCFKELTGLLKKPYSAYNIVHYLTVKYGSNSKTY"
     gene            10831..11664
                     /gene="speE"
                     /locus_tag="PKU88_10380"
     CDS             10831..11664
                     /gene="speE"
                     /locus_tag="PKU88_10380"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPX69720.1"
                     /translation="MDLWIREMQNENAAMTYKVKETLHTEKTKYQELAILDTYEFGRM
                     LVLDGIVQTTIRDEFAYHEMITHVPLFTHGNPKKVLVVGGGDGGTIREILKHKSVEKA
                     VLCEIDDRVVELSKKYLPEISCKLSDPRVEVFIGDGIKYVREHKNEFDVAIIDSTDPI
                     GAAEGLFSADFYKSVFECLREDGIFTAQTEAPFFSPEIVKKVFSDIESIFPITKLYMG
                     TVPTYPGVHWSFTLGSKKNDPEKTDIDSIPELDTKYYHKDLHKACFVLPKYIKNLIMQ
                     K"
     gene            11855..13342
                     /locus_tag="PKU88_10385"
     CDS             11855..13342
                     /locus_tag="PKU88_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX69721.1"
                     /translation="MYKLDQTKTPLFDALLEYHNNDTVPFHVPGHKRGQGMVRKFKDF
                     VGTNVLSIDVTVFHQVDSLHKPTGVIKEAQELAADAYNADYTFFSIHGTSGAIQAMIM
                     SVVTQGDKIIIPRNVHKSVTAGIILSGANPVYMHPEIDARVGLALNVTPETVRKTLEQ
                     NSDAKAVLIVNPTYYGVSTDIVEIAKIVHEYNIPLIVDEAHGPHLHFNSRLPISAMDA
                     GADICAQSTHKIVGSMTQSSLLHVRKGFVDVNRVKTVMSLLQTTSPSYVLLASLDCAR
                     MQMATEGKKLIDDAIELAGYARERINDIKGLYCFGEKVLEKKGAYGFDPTKITITSKD
                     IGLSGHDLEDILTEKYFIQPELSDLYNILCVFSFGDTKEKVDKLINALEEISAEQSCK
                     EIVKSEVLGIPNIPESVLTPRDAFNSMTIPIPLEDSVGLISAEFLMAYPPGIPILCPG
                     EIITQEIVDYVRALKAAKLYVQGTEDPEVNRIKIVSDLQIMNVNM"
     gene            13528..14511
                     /locus_tag="PKU88_10390"
     CDS             13528..14511
                     /locus_tag="PKU88_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPX69722.1"
                     /translation="MKRIIIVFTGGTIAMRIDEKTNAAVPAISGEHILQMTPGLDSIA
                     EIGFLNFSNIPGPHMTPGKMFELSKAIQYKLEVEGYDGAVVTHGTDSLEETAYLVDLT
                     TNSEKPIVFFGSMKNSSELGFDGPMNLIAATYAAVSNEAYGRGVLVVMNNEIHSASQV
                     TKTNTHTLDTFKSADFGPIGFIDNDKAYFYHDYTKRQHIGSEAIDSRVDLIKTACGMD
                     DKLLRFCVDSGTHGIVIEGMGRGNIPPTMVPGVEYALSKGIPVVLVSRCLMGKVLDSY
                     GYPGAGKELTKRGVILGDNLPGQKARIKLIVALGHTKNLAEIKDIFEKNYY"
     gene            complement(14608..14928)
                     /locus_tag="PKU88_10395"
     CDS             complement(14608..14928)
                     /locus_tag="PKU88_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69723.1"
                     /translation="MKEFSNELLQAVKMMGISARTAPKANGLDFLEIKVLNEEETVKL
                     GKAMKEYGLSSGKRNFERDGENVMNSQAGLLISINGEKHAGLNCGACGYNSCSGKNIY
                     FDRK"
     gene            complement(14956..15501)
                     /locus_tag="PKU88_10400"
     CDS             complement(14956..15501)
                     /locus_tag="PKU88_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPX69724.1"
                     /translation="MTFVTSLETSFKKVVSAFKNPWPSMLAVLLTHIGSPLIAWFMGY
                     IFYINDPMIRFGLLIVAAVPMGVTSIIWTGIAGGDVAVSIMAVTLDTIIAPVLLPGFF
                     MAVAGKFIHIDYFQMVIQLLWMVTIPSIVGMRNNAFGMVLALSYFTPAMAISITLSVL
                     FQQPISALIAQVLSCKKTEEF"
     gene            complement(15619..16302)
                     /locus_tag="PKU88_10405"
     CDS             complement(15619..16302)
                     /locus_tag="PKU88_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPX69725.1"
                     /translation="MKGKLIIIEAGSDASGKATQAKRLYSRLRSDKYNIRKIEYPNYK
                     SNSSALVKMYLNGEFGYRPEDVNAYAASTFFSVDRYASYMKEWKGFYEDGGIVLADRY
                     TTSNMVHQASKIADEAERNSYLDWLWDLEFVKLGLPVPDLVIFLDVPPEISNNLITKR
                     NNKITGGSKKDIHESDREYLIKTYLNACRVAEKYGWKRIDCTYDNCLRSIEDIHNEIF
                     EYVIQNLPL"
     gene            complement(16329..17078)
                     /gene="mtnP"
                     /locus_tag="PKU88_10410"
     CDS             complement(16329..17078)
                     /gene="mtnP"
                     /locus_tag="PKU88_10410"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPX69726.1"
                     /translation="MDYKADIGVFGGSGFYSFLEDVEEIDIETPYGSTSEKISLAKTG
                     GKTVAFLPRHGKKHSFPPHMIPYRANVYAMKMLGVRHIIAPTASGSLQAGIKLGEFVI
                     CDQFVDRTWGRKDTYFEGPETKHISAAMPYDENLRNLAIKACEEAGVTVHKKGTVVVI
                     QGPRFSTKAESRWFSKMGWEAINMTQYPECILANELNIPYVNISLITDYDAGLEGDEN
                     IKPVTEEEVYRVFNENNEKVKEVIYKMIEQL"
BASE COUNT         4939 a         3944 c         3291 g         5040 t
ORIGIN      
        1 tcattgaaaa tatatatgct ttccggactg cagcccagtt caacagcctt tttcaaacct
       61 tcatccctct gaacctgtat cgaataccct ctctcggcct gttcctccgt cgatactctc
      121 aaatacacca gcgcagccaa acccttcaca acctccataa tatcttttca ataaccatcc
      181 agatatctta ttataagcct caattctctc tgtctccgat tcttccctta caagtttgat
      241 ttcatattta tctgctttca caataccacc tgcaatatca aatattttga taagacttgt
      301 acctgcacat tattaaagga aggatttccg tccttccttt agctttggta tatcatcaac
      361 cctttttgtt ctttaacatc gagcccctta atattttgtt atctccaaaa cgttcccgta
      421 ttctgtcaat tgttttctca atggtctctt ccttatgtgc attatgctct ccttcaacca
      481 gctcaaatat cgaaagctgc tccgtttcat ccgtgtcaag attgcttaag ccaagcccta
      541 taagccgaat aagcttgttt ttgttcttat tcgtttcaag cagtgccagt gcattggtat
      601 aaatgtcttt cgtcagataa gtaggtgcca ccgttttctg ccttgtaaca gtcttaaagt
      661 cttcatattt tatagaaatt gaaactgttt tccctttata ccctgatttt ctggcttcat
      721 agcctacctc ttcagccaga cccagcaaaa tgtactttaa atattcaagg tcgttggtat
      781 cctgcggcaa tgtggtagac cggctgatgg acttattatc ataaattgga ttgggtacta
      841 ccggagaatt gtctatcccc cttgaaagcc tgtatagttc ctcgccgtat ttcccaaagt
      901 tttttttcag aaattccaca ttgctgtttg caatatcacc tattttatat attgcatagt
      961 ccatcagctt ttgctccgtc tgctttccta taccgtacat ttctcttaca ggcagaggcc
     1021 acagcttttt ttctatgtct tttacataaa gctccgtaat tccctgaggt tttttcagtt
     1081 cggatcccat ctttgcaagg aatttgtttt ccgaaattcc tattgaacac cagagatcca
     1141 gctcttctga aatagtcttc attatgtttt ccgcaatctc cataggactt ccaaagagtt
     1201 tttcacatcc cgtcaggtcc atccatgcct catctatgct gttttgttgt attacaggtg
     1261 tataccttga cagtatatcc ataacctcct tggatttctt ttcatacagc ccatggctcg
     1321 ggggcaccgt tacaagctcg gggcacagct tctttgcttc atgaagcacc attgttgtct
     1381 tgataccgta tttcctggct tcataattag cggcaagtat tattcccgaa cgctttttgg
     1441 ggtctcccgc aacagccgca ggttttccct tcagctcgga attcattgcc atttcgcagc
     1501 ttataaaaaa tgcgttcata tcaaccagga aaaccagcct gttcatcctt tgttcacctc
     1561 cgcagcgaat cccgcacaac agttctcttt ccattatacc aatctatatg gctcaaatgt
     1621 accttttatt tgttcaagag cttatgcagt ttttccttcc gatacttcgc ttacaattat
     1681 tgtttcagaa accggtatat gtgtggttac atcaatttta ttgcttccca ggggaacgac
     1741 tatctgaaca tccgtcttga ccgtcaggta tattttaagc cttgtctgat ttattcccgc
     1801 ctcttcaaag tccgtaaaaa aatctacatt tacactgccc gcaggtcgaa tgttaacacc
     1861 tattacaggg cctgtattgg caaatagctg gctgccgaaa atacttgaca tgggaatttt
     1921 aagatctgtg attccgatct tttttatttc atttaaaatc tctattgagg tttctgccgc
     1981 cagcttggtc atttgtgccg agtttgcctg tatcagggat atccttccat tgttgtcggt
     2041 cttgtagtct ataagcttac tgaatgaatc ggtgattacc ttgcttctta ctgcttcatt
     2101 tattgcctgt gttgcaataa gtcttgcgtt tatctccgac attgcaagta ttgttggcct
     2161 gatatttctt tcaacaatat aatatgaata tattgaaatg ataatcagca gtataaagct
     2221 taagaaaagc ttctttctgc cgcccctttg accgaacttc ctcctcatga gcacacaccc
     2281 cctatttatt aatatatgat tcaaagttat ccatattaca aatatttata tttagtaata
     2341 cattcacatg ctgtgacaaa aatgcatatt ttacgtcaaa ataatatatt caggattaaa
     2401 agagagcaaa aatgtcaaga atcaaaaagg cggcttatat ggcagcagcg ttgaacttca
     2461 ctgggtttgc cagaaaaaat cttctatgat ataataattt cattacatac agcatttata
     2521 cttgcggagg tataaaatga gcgaaaacga gacaaactct cccaagaaaa aaaagaagaa
     2581 aaaaagaacc ttctctgttg gcaggttctt aagatttatc atacttctgc ttctctttat
     2641 aattcttgtg agcggcagca ttgcgctggg tactgcatat tcctgggtaa aagctgcaag
     2701 gccgcttaat gtggatgaat tgtttgactt gaatcagact acatatattg ttgataagaa
     2761 tgataagctc attgacaagc tgcacgccaa tgagaatcgc accatagcca cattggacca
     2821 aatccctgaa tacttgcaga atgcatttat agctattgaa gacaaacgat tcagagagca
     2881 cagaggaatt gacctgtacc gcatctttgg cgccttaaag gccgaccttg ccagcggaga
     2941 gttttcccag ggtgcaagta caattacgca gcagcttatc aagaatgtct accttactcc
     3001 ggacaagaaa tggaaaagaa aagtagttga aatgtattat gcgcttcagc ttgaaagaag
     3061 atttcttaag gatcaaatat tggaagcata cttaaataca atcgcactgg ggcataatgt
     3121 tgccggagtt aaagaagcag ctctttatta ttttggaaaa gagcttgatc aactgacctt
     3181 ggcagaatgt gcaacaatag cgggtattac ccagtatcct tccttatact cgccctattt
     3241 gaattttgaa aaatccatgg gcaaaaaaga aattgtgctt gaagaaatgc ttgtacaagg
     3301 ccttatttct caagaagagt atgatgaggc tatgaagcag gaaatcaaac tgtcaaaagt
     3361 agaaaaggaa gtagaaacaa catattttgc agacatgatt atcagcgatg tcacggaaac
     3421 gctgcagaaa gaactgggac taagcaagga agaagctcaa ataaagcttt ttaacggagg
     3481 acttaagata atagccacta tagatacgga aattcaaaac atagtggaag aaaccttcaa
     3541 gaataccaag ctcttccctg agagtgagga agatgccaac ggcattctcc agcctgaagc
     3601 agccgtaata ataattgaaa acggaaccgg cgaaatcaag gcagttatgg gaggcagaag
     3661 cgagaaggta agaagaggcc tgaacagggc tactcaatcc ttacggcagc cgggttctac
     3721 cataaagcct cttgctgttt atacaccggc tttggataac ggctataccg ccggaactgt
     3781 tatagacgac gctcctgtta tttttgggaa cttcaaaccc cgtaactaca gcaataattt
     3841 taaaggcctt gtaactgcaa gggaagcctt gcagaattca acaaatgtgg ttgcggtcaa
     3901 aatattgcag gatgttggaa tacagcgttc catagaatat ctgaaaaaat tcggaattac
     3961 aactatagtc accagagagg ataacggcgt caccaatgac gaaagccttt cccctcttgc
     4021 ccttggcgga gtgaccaacg gagtcaagcc tatagaaatg gccgctgctt tcagtgtatt
     4081 ccccaataag ggcatttata taaaacccat ctcctttaca aaaattcttg ataagaatgg
     4141 gaatgtgatt tatgaaaaca aaccatataa ggataaggtt atcagcgagc aggttgccta
     4201 tctcatggta gatctcatgc ggggcgttgt aaggggaggc accggcggta atgcagcatt
     4261 atccaagatg cctgttgccg gaaaaacagg tactaccaca aaaaatgtag atgcatggtt
     4321 taccggctac agcccgtatt atacagcctc tgtatggata gggcatgatg aacccaagcc
     4381 tatgaacttc accggaggca gttatcctgc aaagctttgg aaggctgtta tggaagaaat
     4441 ccataaaaac cttgaggtca agaatttcga gaagcccggg ggattggtat ctgtaagcat
     4501 ctgtacggta tccggaaaaa ggccctctga actgtgcgcc cttgacccaa gaggcaccag
     4561 ggtaagatcc gagctgttta ttaaaggcac cgaaccggat gcggaaacta tttgtgatgt
     4621 tcatgtaatc agagatgcct gtacggattc aaaaaaactt gcaacagaat tctgcccggc
     4681 acccagtgta cagtcccagg tatttattca aagaatagag ccttttatcc cggaaccgga
     4741 atcggatcag aaacagcttc cggcagattt gatatatgag gcaccgatag aaccctgcga
     4801 tattcatacc gagccgactt ttgaggagga cccgctggag ggcgagatag acctggaggg
     4861 cgagatagac ctggagggcg aagaagatga agagttttag cataatgaaa taataccgga
     4921 gccgaattaa agcaacagga gcagttagtg tgtatgcact ctgctccttt caattatttc
     4981 cttcattgcc tttgtaatta ttggcgcccc tttgtcgcag cagtccttcc cgtccatctt
     5041 gcccggatca ccgatacctg caatcacagg aacatcaata gaatcaagta tatcaaccgt
     5101 atccccgttt atttttcccc ctgcggttat atttccattc ttgtccactg ttttttccac
     5161 aatatttccg ttctttgtca ccgaaaagtc aatactgact ccttttactc cttcggtatt
     5221 ggatgccacc gcaatcactc ccagtacttc gatgtcgggg ctcctcgcta tttccagcat
     5281 ggctttttcc ccttttcccg ttccggtatg ccctctgtcg tcaaccataa caactaccgg
     5341 atcatatggc gcctgcttaa ccagctctat tattttctga ccgctcaatg gggtcggatt
     5401 gcccgctgac tttgaaatgc atctccctcc tatcctcgtt gcggctgtct ccactgcttt
     5461 tcttgcaatc ccatcgccgt cggtaaccag tatgactctt ttctttgttt cattcataca
     5521 actgtctcct atttttttga tttgggacta aaaagcaatg tcattatata cccgaaaaaa
     5581 atagctcccg ctatacctcc tgcagtactt ttcaagcccc ctgtaaaggc gcccagcaac
     5641 cctgcctcct ttacctcctt cattgcaccc tttgccagag aatacccaaa ccctgtcagc
     5701 ggtgttgttg cacctgctcc tccaaaatcc actattgctt gatatattcc tatcccttga
     5761 agaattgcgc ctgcagttac aaaggcaaca agaaccctgg caggtgtcag tttagtaaga
     5821 tccatcaata gctgtccgat aacgcagatt gctcctccaa ccaaaaatgc cttcaggtaa
     5881 tccatcacac accaactcct attgtttttt ctatggcaac agcatgagct atacacggga
     5941 ttgattcccc ttgccaggtg gtggttggtg aaagcaatgc tccggttgca atcaaaagca
     6001 ctttattaat attacccttc ttcatttctt ttgcaatata accgttcaat acaacggcag
     6061 cacatccgca gccgcttcct cctgcatgag tatcctgttt ttcgctgtca aaaatcattg
     6121 agccgcaatc attataaacc ttagtaatat cataaccttc cttttgcatc atatccaata
     6181 ttatagcctt tcctatgttt cccaagtctc ctgttattat caggtcgtag tactcaggcc
     6241 tcctgcccgt ttcattcaaa tgggctgtaa ttgtatcaac agcagccgga gccattgcag
     6301 cacccatgtt gtttgcatct tttattccaa agtcttgtac ttttccaatg gtagcatgag
     6361 taatatacgg tccggagcct gtctccgcaa ttaccgcagc ccctgctccc gttacggtcc
     6421 attgtgcgaa aggaggcctt tggactccct gctccaaggg aaacctgtac tgcctttcgg
     6481 cactgcagaa atgacttgat gtagcacata ctatcgcttc tgcaaaacct ccgtcaatca
     6541 tcagtgagcc catacataag gattcagcca tagttgagca tgctccgtaa agccctatat
     6601 acggaatctg caattccctt gccgcaaaac tggaagatac tatctgatta agcaaatcac
     6661 ctgcaaacat aaagtcaata tttgtattgc tccttccgga ggctttaaca gcgcttttta
     6721 ctgcttcctt gagcatcttg ctctcagcct tttcccaact atcctctcca aaatagtcat
     6781 ccgtcaatat ttggtcaaag tactgtgaaa gagggccctc accctcctta ggccctacta
     6841 ttgcggcatt ggatatcaca gaagggggtg aagaaagctt cagtgtctgt ttccccagcc
     6901 tttttcctgc catcctatca cttccaaaat aataattttc cttctcattt cccgagcaaa
     6961 taataaatga tacctatcaa aattgaagat gaaataccat aaaccaatac cggccctgca
     7021 agtatgaaca tcttggcccc tactccgaag acaaaaccct ctcttttgaa ttccatagcc
     7081 ggtgatacta tcgaattggc aaagcctgta attggcacta tgcttcccgc acccgcaaat
     7141 ttacctatgc ggtcatatac accgattcct gttaaaaagg ctcccaaaaa caccattgat
     7201 gcagctgtaa tcgatgaaac tgcttcctgg ttatatccat agcttttggc tgtatttgat
     7261 atgaactgcg ccacatcaca tataaagccg cccacaataa acgctgcaat gcaattcttc
     7321 aaatacgccg gcttgggcag tttgtctttt atgtactgtt catattgctt tgaactcatt
     7381 tttgttttca atacatccat gcctcctgtt gcaatgggct attgcctgtg ttcgaatacc
     7441 cttgcgctat tttttattat ttttcctcct atatcgctcc ttatttcctt ttcagtctcg
     7501 ctttcaaagc ccaggatatt tttcagctgc ttgaaccttc tgttactgaa cataataccc
     7561 actcccctgt tctccgaata caagagtcgc cctttcacgg ttttccttaa gcagttttcc
     7621 ggcataataa tcccaggaaa aataacatac ggctattatc acggataata caaggaaaag
     7681 cttaaaaatc ttcattgcgc aacactccat taccctttat gcaattatta ttggctatta
     7741 aggcaaatta atacgaaaaa caaataaaat aaatgaggac atggggacgg ggacacgggg
     7801 acggggacat ggggacgttt cccctgtccc acttccagtg ggacacgcga aacgtccccg
     7861 tgtccccgtc cccatgtccc cgtccccatg tcctcattta catattctgc ctgagcttgc
     7921 tgattatttt cttttcaacc cttgacacct gaacctgtga catccccagc atctgagcaa
     7981 cctggcactg ggtcatgtcc ttgaaatagc gaagcattat aatctggcgc tccttgggct
     8041 ccaatgcaga cagcacctct tttaatgcca gtctgtctat tacttcgatt tcttcccctt
     8101 cattaaagct tattctgtca ataagaagta tcggcgaacc atcatcctga tgtatggttt
     8161 catagagata ctcagtactc tgtaccgatt ccagcgctac taccagatcc tcctttgaaa
     8221 tctcaagtac agatgcaatt tcagatatcg taggctccct tccgtaatcc ttttccagtg
     8281 cttcctttgc gatatgagcc ttgttcgcta tttctttaag attcctgctg acctttatta
     8341 ttccgtcatc cctgagaaat cttttaattt caccaattat cataggtatt gcgtaagtcg
     8401 agaactttac gttatagctc aaatcaaatt tcttgatagc cttcaacagg ccgatgctcc
     8461 ctatctggat aagatcgtca tattcacagc ctctgttcaa atatctcctg acaacgcttc
     8521 ttaccaaacc gatattgttt tcaaccagga aattttctgc ctctgagtct cctctttgtg
     8581 cccgggatat aagttccaaa aactcatcct gtgtaagctc aacccgttcc atagcaatac
     8641 ctcatctgac tgtcgttatt tttttggtca tacgtatggt cgttcctttc caaggaagcg
     8701 attcaatttc cactttatcc ataaaggttt ccatgacagt gaaacccatt cccgatcttt
     8761 caagctccgg cttagatgta tacagaggct gcctggcaag ctcaatatct gctataccga
     8821 ctccgaaatc ctttacttct atctctacgg tatcgccgaa tatccttgaa acaataacca
     8881 cattcccaat ttcgcatgaa taaccgtgta taatggcatt tgtaaccgct tcggacactg
     8941 cagtctttat ctccgccagt tcttctattg tcgggtccag ttgtgccaca aatgctgcaa
     9001 ccacagatct tgcgaagctc tcattctgtg atttgcttag gaattcaagt ttcatttcgt
     9061 tatctgcttt cattgtgtta tcactccttt atacttgcaa tggcttgctt tttgctggaa
     9121 tatatcttaa taatcttctg cagcccgcat atctcgcaca atctcattat ttgaggcttt
     9181 acattaatca tacctgcctt acccccacca ttcgcaataa gcttataccg tcctatcagc
     9241 acacctatcc ccgagctgtc cataaaacct acctgttcca tgtcaaaaag gattgacctt
     9301 gggttctttg aagcaatctc cttatctatt ttggatctta tctcttcaca agaatggtga
     9361 tcaatatctc cattgatgcg aacaaccagc agcctgtccg cataactaaa atctatgctc
     9421 attcatgtcc ctccaattca aattcgtcta tattaatatt ccatacatga cagctttttc
     9481 cttcaaagaa atatgtatag ttttgtaata ccgccaaaaa taaaatgctc ggtttttgtt
     9541 gtaatatata caaatacgca gaaattttcg tttttatatc tatctagtaa aattagcttt
     9601 ttgtgctata ctaataattg ttaatacaga tgagacccat agccaaaata ttttactcgt
     9661 tttaggagca aaaaaatgaa tgtactgatt ttttccgttt ccatcggcaa tggccacgac
     9721 caggttgccc atacactgag aaatgagttt atacgccagg ataactcctc aagagtaagg
     9781 attatcaata caataaatta tatcagccca ttattggata aagtcattct tgacagttac
     9841 ctgaatatat tgaagtttta tccgaaagcc tggggaaaga tttatgaaaa aaccaaccgg
     9901 cttgacccga ttatagacat aaatgatatt accaacagat tgatgaccag caagctcagg
     9961 aaaaccacta taaactttga tccggacatt actatatgca cccacagctt tcctgctgct
    10021 ataatttcaa aacttaaaca gaaaaagaga attggctgcc cccttgtaac tgtagtcaca
    10081 gacttcaata tccattcctc ttatattaac gataataccg actactatgt aatagcccat
    10141 gaatacctta cttatattat ggagagattc ggcgtacaaa aggaaaaggt acttcccttc
    10201 ggaatcccaa tcagaaagga gttcagcgaa tcctataaca gggaagaact gattgcaaag
    10261 ctgggatatg aagataagaa gaccatactt gtaatgggcg gcggccttgg tctgggagat
    10321 ataaacaata tagtgaaagc catcgacagc aatatggaag acattcagat tattgcagtt
    10381 gcaggcagga ataacaggct tgaatcaaag cttaaggatt tgtctgcaaa gaacaggctt
    10441 gtggtatacg gattcgtaaa caacatgcat gagcttatgg agctgtctga ttgcataata
    10501 agcaagcccg gaggagtatc tgtatcggag attttaagca gggaaaaacc cctggtaata
    10561 ttctctcccc ttccggggca ggaacatgag aatgtagagt ttctcctgaa ctcaggtgct
    10621 gcagtttata cctcagatgt cagaaggata cctgctctta ttaatcagat atttgattcc
    10681 gagatcagga tgaaatgctt caaagagctg acaggattat tgaaaaagcc gtattcagct
    10741 tataatatag ttcactacct tacggtaaag tacggttcta acagcaaaac ctattaaaat
    10801 attaataaat atagattgga ggaaacttaa atggatttat ggattaggga aatgcagaat
    10861 gagaatgccg caatgacata caaggtgaaa gaaaccctgc ataccgaaaa gacgaagtat
    10921 caggagcttg ccatactgga tacctatgaa ttcggcagaa tgcttgtact ggatggaatt
    10981 gtccagacaa ccatccggga tgaatttgca tatcatgaga tgattactca tgtaccgctt
    11041 tttactcacg gtaatccgaa aaaggtttta gtagttggcg gcggtgacgg aggtacaatc
    11101 cgggagatac ttaagcacaa gtccgttgaa aaagcagtat tatgtgaaat tgatgacaga
    11161 gtagtagagc tttcaaaaaa gtatcttcct gagataagct gcaagctttc cgatccccgg
    11221 gttgaagtct ttatcggcga cggcataaaa tatgtgcggg agcataaaaa tgagtttgat
    11281 gtggcaatca ttgactcaac agatccgatt ggtgctgccg aaggactttt tagcgccgat
    11341 ttttataagt cggtatttga atgcctgaga gaagacggaa tttttacagc tcagacagag
    11401 gctcccttct tttcaccgga gatagtaaaa aaggttttta gcgacattga gagtatattc
    11461 ccgattacca agctttatat gggcaccgta ccaacatacc ccggagttca ctggagtttt
    11521 acattgggct ctaagaaaaa tgaccctgaa aagacagata ttgacagtat tccagaactt
    11581 gatacgaaat actatcataa ggacctgcat aaagcatgtt tcgtacttcc aaaatatata
    11641 aagaatttga ttatgcaaaa ataaaattaa aatacaaatt ttgaataata tttattgcat
    11701 tttatatagg tttgccctat aataaatttg gcatttgaat aacacaacta aaaatcgctc
    11761 tgcagaagcc tgaacttatt gcatcgaagt tcgtggcttt ttctttagca tattatttgg
    11821 acatacttta gataaccgaa aggaagtgta accgatgtac aagttggacc agacaaaaac
    11881 ccctttgttt gacgcattgc tggaatatca taacaatgat actgtccctt ttcacgttcc
    11941 ggggcataag cgcgggcagg gaatggtcag gaagttcaag gattttgtgg gtacaaacgt
    12001 cttatcaatc gatgtaacag tttttcacca ggttgacagc cttcataagc cgacgggcgt
    12061 tattaaggaa gcccaggaac ttgccgccga tgcttataat gcagattaca cttttttttc
    12121 aatacacgga acctccggtg caatacaggc aatgataatg agtgttgtta cacagggaga
    12181 caaaataata ataccaagaa acgttcataa atcagttacg gcaggtatta ttctcagcgg
    12241 tgccaatccc gtatatatgc atcctgaaat tgacgcaagg gtaggattag ctcttaatgt
    12301 tacccccgag actgtcagaa aaacactgga acagaacagt gacgccaagg cagtgcttat
    12361 tgtgaatcct acttattacg gtgtatcaac cgatattgtt gaaattgcca aaatagttca
    12421 tgaatataat attcctctta tagttgatga agcccacggc cctcatcttc acttcaacag
    12481 caggcttccc atttcagcca tggatgcagg tgcagatata tgtgcccaaa gtacccataa
    12541 aatagtgggc tccatgacac aaagttcact gctgcatgtg agaaaaggat ttgttgatgt
    12601 caacagagtt aagaccgtca tgagcctcct gcagactaca agcccctcat atgtgcttct
    12661 ggcctccctt gattgtgcca gaatgcagat ggctactgaa ggtaaaaagc ttatagatga
    12721 tgcaatagag ctggccggat atgcaagaga aagaataaat gatattaaag gcttgtactg
    12781 ttttggtgaa aaggtccttg agaaaaaagg cgcctacggc tttgacccta caaagattac
    12841 cattacttca aaggatatag gcctatccgg tcatgatctt gaagacatac tgacggaaaa
    12901 atatttcata cagcctgaac tgtcagacct ctacaatatc ctttgcgtat tctccttcgg
    12961 ggatacaaaa gaaaaggtag acaaactgat aaacgctctt gaagaaataa gcgccgaaca
    13021 aagctgcaag gaaattgtaa agtctgaggt attgggaata cccaatatac cggaaagtgt
    13081 cctgacaccg agggatgcat ttaacagcat gacaataccc attcccctgg aagacagtgt
    13141 gggactcatc agtgccgaat tcctgatggc ctatcctccg ggtataccca tactttgccc
    13201 cggggaaata ataacacagg aaattgttga ttatgtaagg gctttgaaag ctgcaaagct
    13261 ttatgtccaa ggtacggaag atcctgaggt gaaccgcatc aagatagtca gcgacctgca
    13321 aattatgaat gtaaatatgt aatattattc tattgtatag gaatgtataa ctttcctata
    13381 caatggggga tgcaaggggg agaagccccc tgccgataaa ggagggtgct caggatgcgc
    13441 tttgcatccg tagaagggaa ccatagggtt ccctagcgaa aaaaattcgg cgtgagacgt
    13501 attttttata gggcagggag gaagagtatg aaaaggataa tcatagtatt tacaggcggc
    13561 accattgcaa tgagaattga tgagaaaacc aatgcggcgg tgcctgcaat aagcggagag
    13621 catatacttc aaatgactcc ggggcttgat agcatagcgg aaatcggctt tctgaatttt
    13681 agcaatatac cgggcccgca tatgactcct ggaaaaatgt ttgagctttc caaggcaata
    13741 cagtacaagc tggaagttga aggctatgac ggcgcagtag taactcacgg taccgattcc
    13801 ctggaagaga ctgcatacct tgtagacctt acaaccaact ccgaaaaacc catcgtgttt
    13861 ttcggctcta tgaaaaacag ctcggagctg ggctttgacg gaccaatgaa cttaatcgcc
    13921 gcaacctatg ccgcagtttc aaatgaagct tacggcagag gtgtactggt agtaatgaat
    13981 aacgaaatac attccgcctc acaggtaacc aagaccaaca cccatactct ggataccttc
    14041 aagagtgcgg atttcggccc cataggtttt attgacaacg ataaagcata cttctaccat
    14101 gactatacaa aaaggcagca tatcggctcg gaagcaatag acagccgtgt agacctgatt
    14161 aagactgcct gcggtatgga tgacaagctg cttcgttttt gtgtggattc cggaacccac
    14221 ggaatagtaa ttgaaggtat gggcagagga aacatcccgc cgactatggt tcccggggtt
    14281 gaatacgcat tatccaaagg aatacctgtt gtacttgtat cccgctgtct tatgggcaag
    14341 gttctggaca gctatggata tccgggggcg ggcaaagagc ttaccaagag aggcgtgatt
    14401 cttggagaca acctccccgg gcaaaaggcc aggatcaaac ttattgtggc gttaggccat
    14461 acgaaaaatc tggctgaaat aaaggatata ttcgagaaga attattattg aaattcacct
    14521 tatgggatta cctcgagagg ccaatgtgga aacatgttgg gctctctaat tatcagccgg
    14581 ctaataaata gtcttaggta tgtaatatta tttgcggtca aaataaatgt ttttaccgct
    14641 gcaggaatta tagccgcatg caccgcaatt caaccctgca tgcttctcac cgtttatgga
    14701 tataagcaaa ccagcctgtg aattcattac attttctcca tcacgttcaa aattccgttt
    14761 gccgctgctt aaaccatatt ccttcatagc ttttcccagt ttaacagttt cttcttcatt
    14821 tagaacttta atctcaagaa agtccaggcc attagctttt ggcgcagtac gggcggaaat
    14881 ccccatcatt ttcactgctt gaagcagctc attactaaat tctttcattt atatacctcc
    14941 ttcagatcaa actaattaaa attcttctgt tttcttgcag gataaaactt gtgcaattaa
    15001 tgcagaaata ggctgctgaa ataacacact tagtgttatc gaaatagcca tagccggtgt
    15061 aaagtatgac agggcaagca ccatcccaaa ggcgttatta cgcataccaa ctatactggg
    15121 tatcgtcacc atccataaaa gctgtataac catctgaaaa taatcgatat gaataaattt
    15181 tccggcaacc gccataaaaa aaccgggaag cagaacaggt gcaataatgg tatccaaagt
    15241 cacagccata atagacacag ctacatcacc cccggcaatg cctgtccata ttatggaagt
    15301 tacacccata ggcaccgctg ccactatcaa caatccaaag cgaatcatag gatcattaat
    15361 gtaaaatata tatcccataa accatgctat aaggggtgaa ccaatatgtg tcagcagtac
    15421 ggccagcatt gaaggccacg gattcttgaa ggcactgaca acttttttga agctggtctc
    15481 aagtgacgtt acaaatgtca tataagcaaa aaatactatg gaaagcatct ttgtattatt
    15541 aacttaatat tatcattcac aagccactga taatgcatga ttattactgc atgcagggca
    15601 aatcgaagaa cctcccgttt aaagcgggag gttttgtatt acatattcaa atatttcatt
    15661 gtgtatgtct tctatgctcc tcaagcaatt atcataagtg cagtcaatcc ttttccagcc
    15721 gtatttctct gctacccggc aggcgttcag atatgttttt ataaggtatt ccctgtcgct
    15781 ttcatgtatg tcctttttgg aaccgcctgt tatcttatta tttcgtttcg ttatcaggtt
    15841 attgcttatt tcaggcggta catccagaaa aatcaccaga tccggaacag gcaatcccag
    15901 ctttacaaat tccagatccc agagccaatc cagatatgaa ttgcgctcgg cttcatcagc
    15961 tatctttgaa gcttgatgca ccatattgga ggttgtatac ctgtctgcca gcactatacc
    16021 tccgtcctcg tagaacccct tccattcctt catataggaa gcatatcggt ccacggaaaa
    16081 aaaagttgat gcggcgtaag catttacgtc ctccggcctg tagccgaatt ctccgttcag
    16141 gtacattttc accaatgcag aagaattgct tttatagttg ggatattcga tcttccttat
    16201 attatattta tcgctcctca gcctgctgta aagccttttg gcctgagtag ccttcccgct
    16261 ggcatcactg ccggcctcga taataatgag ttttcccttc atatttctac tctccaatat
    16321 aacgcatatt aaagctgctc aatcatttta tatataactt cctttacttt ttcattgttt
    16381 tcattgaata ccctgtatac ctcttcctct gtaacgggct ttatgttctc atccccttca
    16441 aggcctgcat cataatcggt tataagggaa atattcacat atggtatatt cagctcatta
    16501 gccaggatgc attccggata ttgagtcata ttgattgctt cccagcccat tttactgaac
    16561 catctgcttt cagcctttgt tgaaaatctt ggcccttgta ttacaacgac cgttcccttt
    16621 ttatgcacgg taacccctgc ttcttcacag gcctttattg ccagattcct caggttctcg
    16681 tcatagggca ttgccgcact tatatgcttg gtttcagggc cctcaaaata ggtatctttt
    16741 ctgccccatg tcctgtcaac aaactgatcg catattacaa attcacccag cttaatgcct
    16801 gcctgaaggc tccccgatgc ggtaggtgca attatgtgcc ttactcccag catcttcatg
    16861 gcatatacgt ttgccctgta aggtatcata tgaggcggaa atgaatgctt cttgccgtgc
    16921 cttggcagaa aagcaaccgt cttgccgcct gttttggcca gagaaatctt ctcgcttgta
    16981 cttccatatg gggtttctat atcaatctcc tcaacatcct ccagaaagga ataaaagcct
    17041 gaacctccaa aaacaccgat atcagcttta taatccatac tctgatgcct ccttcttctt
    17101 tttacactaa ttaaatatta ctataaaagg cccgcataaa caagctgtat aaaaacttaa
    17161 ggacatgggg acgtttctcc tgtcccgctg aaagcgggac aggagaaacg tccc
//
LOCUS       DAOOAB010000054        16660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_4531_length_16660_cov_18.749473, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000054 DAOOAB010000000
VERSION     DAOOAB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_16660_cov_18.749473"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="PKU88_10415"
     CDS             complement(<1..2508)
                     /locus_tag="PKU88_10415"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPX69727.1"
                     /translation="MNPQKYKTEKLVPINLPEICAPRTQLMDVYRKASEKRCIYVHAP
                     AGCGKTVSTLLWLKESEYKTIWIGLDEYDNTLAGFYRTFCMALFSIVPYEENLAELIK
                     APEFSSSPVEYAIEILSLLSFEDCTYALVFDDFHLITNKEIIKSLIYVFKRLPLSVTI
                     MVLSRNELPETFSQYEESGKIACIGADEQAFKGDEIRRHFASYGRFLTEREAEEVYAL
                     TEGWAIAVNALAMSGDFYPGEKSPSNPLRKYIKAQIWDKLDRDLRLFMLQTAVVDRFS
                     EELCRKITDNPESQQILLYLLGENMFLSRRETEYRYHNLFLDFLREELLEELQINRQA
                     LYEKAANYYYDSGDFFNALCFYIKCNCSKGIADALYHFMKFNVQSSSEMSKVYFINKL
                     PAEILEQNPCLYVSCAWCALLFGDAAELHFYSDKLYDRIYDIVRENKALLEISLFVYS
                     ADQRYTFTEQLAKLQAAAVPLDADPANIPKIISHNMPYFHRTYRDYSHYALNTEEHFA
                     EFSLVFSSLLGSEYAVIESGIRAGLLYDQNRIQDALSLVVENPASVSPELEFLSRMQI
                     AFCLYAMGKAEKTAQCRKEIEAFLKTKKLLYLLPVFSAYETKLKLLDGDSSAAKGWLE
                     NYFITESYNPEMHKIFLHFTTVRAYIVLGEYEKAELLCEKLWKLCTDYHRLLDAAEAA
                     VLLTVILRLTDREAKASALLRKTLLDMEPYGYIRVFADEGNAILPVLKKLIKKADTEN
                     GTVMPGSAYMKAVYMSVYEQSKRYKGIITNMGRKQVKLSAQQKHMIELLAKGYKNAEV
                     VELTGLSINTVRYHTKLAYQKLDVSNAKDAVLRAKELS"
     gene            2719..3060
                     /locus_tag="PKU88_10420"
     CDS             2719..3060
                     /locus_tag="PKU88_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPX69728.1"
                     /translation="MERLLDKSFDFSVRITELIKYLERENKSFPLSGRLLECAAGIGV
                     CMKQAASAGSMSFETHQKTMSYASEAEYLLELMEKTEYMTEKQSRPILGDCRGLMSAI
                     AALNIKQKGRD"
     gene            3062..4411
                     /locus_tag="PKU88_10425"
     CDS             3062..4411
                     /locus_tag="PKU88_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69729.1"
                     /translation="MSKASLIVVSILIIVCSVLFLSSCSARGPETPDVNPAAPAQAPA
                     VEAANQNTAESEAVNQDTAAAETEQQNTAEAEAEQQSTAEAEAVPDSGLTLEKLKAKV
                     QEHGYEVTPVEGYQLTDSIKPEDGFNLHYVDDNTDGFVPVFEFGSSNDALKYAEGVNS
                     AGYNLCIVNGRFVTMTGAQYGIVTYDPQADFLAGLLESEVMAAPKISGSPVIPPASDY
                     AGACKQIDSVRNVLNTLVNRTVLTHDKSAAEDKNFSAAFISFNILSSGNLGFTATLSE
                     DEASRDAMAQMWTAFGVTDMKIERTAPHTYELTGTRAGMEAPFLIRCMFEPSSGALRL
                     TEMDGDRAAELFEYVPLGGDTYAFQTINERAVVKYQGGQITGFTYSLLKSGTAAYSPD
                     ADGIYNKTGLDAAWVTSAGADLFEQLVTYDGTILKIYAESFFTGSRITADINVPQNP"
     gene            4473..6005
                     /locus_tag="PKU88_10430"
     CDS             4473..6005
                     /locus_tag="PKU88_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69730.1"
                     /translation="MKKKLLTLLLILVMASGFTSLSVLAASPPTTISAPENFAAADYM
                     GSSVYCTLSAPEDLRRLIVQTDEERGYYMSIMAQVDFKTDGGSWHYSPEWDNRDTYSK
                     YVLYFYNSLTGGDGGIFLGGEYLVFRNMFPEESNIPVESGFNSWDWYKSHSMTLRARF
                     AIIFGDNVVFSDWSEEYTLSSSSKMGYKKIMNENAPTLVSSKIEARGPDNVPWVVLLL
                     AQHPAAAQKFNAASFDNMWTEIWLRKLGDTDFIKVGAERFHNERISIDIGAYFQENLA
                     SYDAQAYEVKVRYTIDERSYPQSGVTSETWLHSPFSNTLSYGMPVWSAASNWAADELK
                     KADESGLIPDILKNTDLTKPITREEFCELALVLYEKTTGKTPAPYAPNPFIDTTNPQI
                     LKAFALGITKGTSATTFEPNKTITRQECATMLFRTIKGIVPEADYSITGVPDFPDQKN
                     IDSWAVEGTKYMSKLGIIKGDDKGYFMPKATTTAQQATGYGTATREAAILMSVRTYGV
                     MP"
     gene            6002..6697
                     /locus_tag="PKU88_10435"
     CDS             6002..6697
                     /locus_tag="PKU88_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69731.1"
                     /translation="MMRHTFRYLVIIAAAGLLFLCACSPQDQTGENTSHASSASAQQE
                     ETNTSSKEETAATSEESAAEVECNWTLEVSDTVTTQLNGYDFKCTLAVMAVKLGGKDE
                     LGTYRGIVTLKYQYDMKQGNVAGNAAGEGQEIDAVIEVVQYEEQAYNEATGQTVLAEL
                     VQYDAMAIGSLVLTGSGEAAESAGGASWSTSDRKTIPVPYRMAVDGGKVTIELYTIAP
                     GVKFNGMITGTPI"
     gene            complement(6767..7525)
                     /locus_tag="PKU88_10440"
     CDS             complement(6767..7525)
                     /locus_tag="PKU88_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPX69732.1"
                     /translation="MKFGKSLVFILAVILLTTSVTFADVLKNGCRGEEVSRIQSQLKE
                     KGYFTEEVTGYFGNVTEEAVRKFQEDNNLTVDGIVGPDTGKVLFGTEWTYTAEVEALD
                     WWSEASKIFERGTVAKVTDVRTGETFEIVRTYGGNHADCEARTAEDSKKIKEIWGGWS
                     WERRPIVVEVDGRKIAASMAAMPHAGVDKSPADKYISSRSGGYGRGANLDKIKNNGMS
                     GVVDVHFLNSRTHNTNKVDNKHQACVKEAAASGN"
     gene            complement(7664..8047)
                     /locus_tag="PKU88_10445"
     CDS             complement(7664..8047)
                     /locus_tag="PKU88_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69733.1"
                     /translation="MDTEIVLPQEGRERAGTAEAKLAGRMLLTSSIIVNVIEEDTFLV
                     LASVLSVIASLLTIKAARLEAEEQQIAPGVTTFANSLKLIGSHTGLFVSIILLWALLI
                     EISIKQTTELSAQPEMAGTLGAFMV"
     gene            complement(8726..10891)
                     /locus_tag="PKU88_10450"
                     /pseudo
     CDS             complement(8726..10891)
                     /locus_tag="PKU88_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017810617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(10924..11292)
                     /locus_tag="PKU88_10455"
     CDS             complement(10924..11292)
                     /locus_tag="PKU88_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPX69734.1"
                     /translation="MEENRVSIESCSCSMIHEDAVSKVRNSMPEEETLYDLAELFKVF
                     GDTTRIKILCALFESEMCVCDIAALLSMNQSAISHQLRVLKQARLVKYRKEGKVVYYS
                     LDDEHVKQIFDQGLVHIRER"
     gene            complement(11882..12631)
                     /locus_tag="PKU88_10460"
     CDS             complement(11882..12631)
                     /locus_tag="PKU88_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69735.1"
                     /translation="MGETISEKTEGRVSYHDSGEEMLPRIREDGMSNAFDRYAQQEKI
                     RCKFCLQGLSCQLCTNGPCRISEKSGQDKGVCGIGGDTMAMRNMLLRNIMGAGTYSHH
                     AYEAFRTLRETAQGKTPFKITDANKLKWMCDTVGIDSKQDVNKMAGEIADHLDVDIPQ
                     LPIAVTAPEWMEQKATIDGFFAAAYGAYTRLSPIPFVTGAPGLVKLLTEDVEGLTGGK
                     IALGDDPAEAANNIEAHIISKRKGLGLREAD"
     gene            complement(12759..13214)
                     /locus_tag="PKU88_10465"
     CDS             complement(12759..13214)
                     /locus_tag="PKU88_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPX69736.1"
                     /translation="MKRIVILLLAVLLGAAVFSACSTKPQQDISQSPAEIVQPEQSPA
                     EVVQPESISPEEAYKRLESEEDIILLDVRTQGEYEEQHIPGSILIPVNDLEKRAEAEL
                     TDKDADIIVYCASGKRSSSAANILAGLGYSKVYNMGGIMDWPYELESGE"
     gene            complement(13231..13809)
                     /locus_tag="PKU88_10470"
     CDS             complement(13231..13809)
                     /locus_tag="PKU88_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPX69737.1"
                     /translation="MVNTIPSRAAALELLQEYNKNDSLVKHALTVESVMRHFAGLLGE
                     DEEKWGIIGLLHDIDYEMYPEQHCIKAKEILTERGWPEDYIHAVLSHGWKLCSDVEPT
                     EKMEKILYTIDELTGLIAATAIMRPSRSILDMEVKSVKKKWKQKSFAAGVNREVIEEG
                     AALLGMEMDTIIGETINGMKKAAEEIGLKGSL"
     gene            complement(13846..14793)
                     /locus_tag="PKU88_10475"
     CDS             complement(13846..14793)
                     /locus_tag="PKU88_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPX69738.1"
                     /translation="MADKIATREAYGNALAEFGSDNRIVVFDADLSKSTKTDNFRKKY
                     PERFFNMGIAEGNMMATAAGMASCGKVVFASTFAMFAAGRAFEQIRNSICYPKLNVKI
                     GATHAGLSVGEDGASHQAIEDIAIMRALPNMTVISPADAVETKYAVKAAIEHEGPVYL
                     RLGRLNVPVIYNESSYKFEIGKGIVLAEGNDAAIIATGLMVSAALEAKELLQQEGINA
                     RVIDIHTIKPIDADIIVKAARETGAIVTAEEHNIIGGLGSAVAEVLSENYPVPLVRVG
                     VQDKFGKSGKPDILLQMYGLTAADIAKSTKAVLKKKAGR"
     gene            complement(14795..15640)
                     /locus_tag="PKU88_10480"
     CDS             complement(14795..15640)
                     /locus_tag="PKU88_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPX69739.1"
                     /translation="MKKPEIKDLKKYAVKVRKHIIDEVYSAASGHPGGSLSCTEILTV
                     LYFAEMRVNTKQPLWEDRDRFVLSKGHCSPALYAVLAEKGFFPKEDLLTFRKADSYLE
                     GHPSMRYVPGVDMSTGSLGQGISAAVGMAMAGKLDKKDCRVYAVLGDGELQEGEVWEA
                     FMAAAHYKLDNLTAFLDHNGLQIDGRITDVMSPEPVADKFKAFGWNVLKTDGHDIEKI
                     LESIGQAKDCKGKPTIIIAETVKGKGVSYMENQAGWHGSAPNKEQRDQAMAELDKVLA
                     ELEVE"
     gene            complement(15667..16086)
                     /locus_tag="PKU88_10485"
     CDS             complement(15667..16086)
                     /locus_tag="PKU88_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPX69740.1"
                     /translation="MKHTKIGSKYFVRIDKGEELVASLYKFCTDNGLKLGSVIGIGAA
                     NRITIGVFNTVTKEYHNQELTGEYEITNLTGSITEKDGEVYLHLHITLGDEEYNAHGG
                     HLNQCWISGTCELIIDAVDGEIGRVFDDYCGLNIWKL"
     gene            16276..16470
                     /locus_tag="PKU88_10490"
     CDS             16276..16470
                     /locus_tag="PKU88_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69741.1"
                     /translation="MIKIDNDTVRFEGYELENLVVLLEQVVRNSIIDSDSREFAISFL
                     KAFSDCFGEDVIKKFNSIRK"
BASE COUNT         4331 a         4232 c         3387 g         4710 t
ORIGIN      
        1 actgagttct tttgcccgga gcactgcatc cttcgcattg cttacatcca gcttttgata
       61 ggccaatttg gtatggtatc ggacagtatt aatagaaagg ccggtcagtt caacgacttc
      121 agcattttta tagcctttag ccagtaattc gatcatatgt ttttgctgtg ctgaaagctt
      181 tacctgtttt cggcccatgt tggtaattat ccctttgtaa cgctttgact gctcataaac
      241 cgacatgtac acagctttca tataagcgct gccgggcata accgttccat tttccgtatc
      301 agcctttttt atcagttttt ttagtaccgg taaaatcgca ttaccctcat ccgcaaatac
      361 tctgatataa ccgtagggct ccatatccaa cagggttttt cgaagaaggg cggaagcttt
      421 tgcttctctg tccgtcaacc gcagaataac ggtcagcagc acggcggctt cagccgcatc
      481 aagaagtctg tgataatccg tacacagctt ccaaagcttt tcgcaaagta attccgcttt
      541 ttcatattct ccaagaacga tataagccct tacagttgta aaatgcagaa aaatcttatg
      601 catctcagga ttataggatt cggtaatgaa ataattttca agccatccct ttgccgcgct
      661 gctgtcgccg tccaggagct tcagctttgt ttcataagct gagaatacag gaagcaggta
      721 caacagtttt ttcgttttta aaaaagcttc aatttctttc ctgcattggg cggtcttctc
      781 cgctttgccc attgcgtata ggcagaaggc aatctgcatt ctggacagaa attcgagttc
      841 aggtgaaacg gaagccggat tttcaaccac aagtgaaagc gcatcctgaa ttctgttttg
      901 gtcatacagc agtccggcac gtattcccga ttcaataact gcatactcac tgcccaaaag
      961 agatgaaaac acaagagaga attcggcgaa atgctcctca gtattcagtg cataatggga
     1021 atagtccctg taagttctgt ggaaataagg catattatgg ctgattattt tcggtatatt
     1081 cgcagggtct gcatcaaggg ggacagcagc tgcctgcaat tttgcaagct gttccgtaaa
     1141 cgtatatctt tgatctgccg aatacacaaa aagagagatt tccagcagag ctttgttctc
     1201 cctgacaata tcataaatcc tgtcatacag cttgtccgaa taaaaatgca gttctgcagc
     1261 atcgccgaac aaaagagcac accaagcaca gctgacatat aagcacggat tttgctccag
     1321 gatttctgcc ggcagcttgt taatgaaata cactttggac atctccgagc ttgattgaac
     1381 gttgaacttc ataaagtgat acaaggcatc ggcaatcccc ttgctgcaat tacactttat
     1441 ataaaaacaa agtgcgttga aaaaatcccc tgaatcataa taataattag ccgccttttc
     1501 atacagagcc tgccgattta tttggagctc ttccaataac tcctcccgca agaaatccag
     1561 aaacaaatta tggtatctgt attctgtttc ccgacggctg agaaacatat tttcgccgag
     1621 caaatagaga agaatttgct ggctttccgg gttgtcagta atcttccggc aaagctcttc
     1681 agaaaatcta tccacaacgg cggtctgcag cataaacagg cgcaaatccc tgtcaagctt
     1741 atcccatatt tgtgccttta tatatttgcg cagaggattg cttggagatt tttcccccgg
     1801 ataaaaatct ccgctcattg ccagagcgtt taccgcgata gcccagcctt cggttaaagc
     1861 gtacacttct tcagcctccc tctcggtaag gaaacggcca tagcttgcga aatgccgccg
     1921 tatttcatct cctttaaatg cctgctcatc agcaccgata caagcaattt ttccgctttc
     1981 ttcgtattgg gaaaaggtct ccggcagctc gttgcggctt aataccatga tagtaacgga
     2041 taacggaagc cgtttaaaaa cataaatcag agacttgata atttctttat tggtaatgag
     2101 atggaagtca tcaaacacaa gggcgtatgt acaatcctca aaagaaagca acgagaggat
     2161 ctcgatggcg tattcaaccg gtgatgaact aaattcaggg gctttgatca gttccgccag
     2221 attttcctca taggggacta tagaaaagag cgccatacag aaagttcgat aaaagcctgc
     2281 cagcgtgtta tcatattcgt caagaccaat ccagatggtt ttgtattccg actccttcag
     2341 ccacaaaagg gtagatacgg tttttccgca gccggcaggc gcatgtacgt aaatacatct
     2401 tttttcgctg gctttcctgt aaacatccat caattgagta cgcggagcac atatctcagg
     2461 aaggtttatc ggaacaagct tttcggtttt atatttctga gggttcataa aacacctcct
     2521 ttttttgtta ttattatact tagtatatca aaatttacaa aatattcaat cgagttaggc
     2581 atatattccc acattattgt tttgagtctc ccaaaataat tttcaattat cccacatcaa
     2641 tgcggtttta taatcataaa taccatatta aaatgaaata aagataccgg accctattac
     2701 ggaaaggaga tgtcagaagt ggagcggctt cttgacaaaa gctttgattt ttctgttcgc
     2761 atcacagagc tgattaaata ccttgagaga gagaataaga gctttccttt gagcgggagg
     2821 ctgcttgaat gcgcagccgg aataggcgta tgcatgaaac aggccgcatc tgccggcagt
     2881 atgtcctttg aaacccatca gaaaaccatg agctatgctt cagaagcgga atatctgctg
     2941 gaactgatgg aaaaaacaga atacatgact gaaaagcaaa gcaggcctat ccttggcgat
     3001 tgccgggggc ttatgtcggc tattgcagct ctgaacatca aacagaaagg aagggattaa
     3061 catgagtaag gcgagtttaa tagttgtctc aatcttaatc atagtttgtt ccgtactgtt
     3121 cttatcctca tgctccgcaa ggggccctga gactccggat gtcaatcccg cagcgccggc
     3181 acaagcgcct gcggtggaag ccgcaaatca aaatacggca gagtcagagg ctgtaaatca
     3241 ggatacagca gcagcagaaa ctgagcaaca aaatacagca gaggcagaag ctgagcagca
     3301 aagcacggca gaggcagaag ctgtacccga cagcggcctg acactggaga aacttaaggc
     3361 gaaggtgcaa gagcatggat acgaagtaac acccgttgag ggttatcagc ttacggacag
     3421 cataaagcct gaagacggct tcaacctgca ctatgtagac gataacaccg acgggtttgt
     3481 cccggtgttt gaattcggca gctccaatga tgcattgaaa tatgccgagg gcgtaaactc
     3541 ggcgggatat aatctttgca tcgtcaacgg ccgctttgtc acaatgacgg gggcacagta
     3601 cggcatcgtg acatacgacc ctcaggcgga cttccttgcc ggtctgctcg aaagcgaggt
     3661 tatggcagcc ccgaaaattt ccggcagtcc tgttatacct ccggccagtg attatgccgg
     3721 cgcctgcaaa cagattgaca gtgtcagaaa tgtgcttaat acactcgtca acagaactgt
     3781 attaactcac gataagagcg ccgcggaaga taagaacttt tcagctgcgt ttatcagctt
     3841 taacatactt tcttcgggta atcttgggtt tacggctact ctgagcgagg acgaagccag
     3901 ccgcgatgct atggctcaga tgtggaccgc cttcggcgta acggatatga agattgaaag
     3961 aaccgccccg catacttacg aactgacggg aacccgggca ggaatggaag caccgtttct
     4021 gatccgctgc atgttcgaac cgtcctccgg agccctgcgg cttacagaga tggacggtga
     4081 ccgggcagct gagctgtttg aatatgttcc gttgggcggg gatacctatg cctttcaaac
     4141 aattaacgag cgtgccgtcg taaagtatca ggggggccag atcaccggct tcacttattc
     4201 gcttttaaaa tccgggactg ctgcatacag ccccgacgca gacggtattt ataacaaaac
     4261 cggacttgac gcggcgtggg taacgtcagc cggagcggac ctcttcgagc agcttgtcac
     4321 ctacgacgga acgattctga aaatctacgc cgaaagtttc tttacaggca gccgcattac
     4381 agcggatata aatgtgcctc aaaatcctta atgcaccaat taccgaaatt tcgtgaaatt
     4441 ttataaaaat aaatatttaa ggaggcgtcc aaatgaaaaa gaagttactg acattattat
     4501 tgatacttgt aatggcttcg ggttttacct ccctttcggt gctggcagca tccccgccca
     4561 caacgatttc cgcgcccgaa aactttgccg ctgccgatta catgggcagt tccgtctact
     4621 gcaccctgag cgcgccggag gacttgcgca ggctcattgt ccagacggat gaggaacggg
     4681 gctattacat gtcgattatg gcccaggtgg atttcaaaac agatggagga agctggcatt
     4741 attcaccgga gtgggacaac agggacacct attcgaaata cgtgctgtat ttttacaaca
     4801 gtctgaccgg cggtgacggc ggaatatttc tgggcggcga gtatctcgtt ttcagaaata
     4861 tgttcccgga ggaatcaaat attcctgtcg agtcaggctt taacagctgg gactggtaca
     4921 aaagccactc catgacactg cgggcacgct ttgccatcat attcggagat aacgttgtgt
     4981 tttccgactg gtcggaggag tataccctgt caagcagcag taaaatgggc tataaaaaaa
     5041 ttatgaatga aaacgcaccc accctcgtgt cctccaaaat tgaagcgcgg ggcccggaca
     5101 acgtcccttg ggttgtttta ctgttagcgc agcatcccgc tgcggcgcag aagttcaatg
     5161 cggcctcatt cgacaatatg tggactgaaa tctggcttcg caagcttggc gataccgatt
     5221 tcataaaagt cggcgctgaa cggttccata atgaaagaat cagcattgac ataggcgctt
     5281 attttcagga aaaccttgcc agctacgatg cacaggccta cgaggtcaag gtgcgctaca
     5341 caatagacga acggtcttac cctcaatcag gcgtaacatc ggaaacttgg ctgcacagcc
     5401 ccttttccaa tacgctttct tatggaatgc ccgtgtggag cgcggcttcc aactgggctg
     5461 cggatgaact gaaaaaggct gatgagtccg gcctcattcc cgatattctt aaaaatacgg
     5521 atttgacaaa gcccattacg cgggaagaat tctgcgaact ggcgctggta ctgtacgaaa
     5581 agactacggg caaaactccc gcgccttacg cccccaaccc ctttatcgat acgacaaatc
     5641 cgcagattct gaaagctttt gccttaggca tcacaaaagg tacatcagca accacctttg
     5701 aacccaataa aaccataacc aggcaggagt gcgccacaat gctgttcagg acaatcaaag
     5761 gcatcgtgcc ggaagccgac tattccatta ccggtgtgcc tgatttcccg gatcagaaga
     5821 atatcgactc atgggcggtt gaaggcacga aatatatgtc aaagctgggt atcatcaaag
     5881 gcgatgataa aggctacttc atgcctaaag caacgacaac cgcgcagcag gcaaccggct
     5941 acggcacggc gacaagggag gcagcgatcc tcatgagtgt gaggacttac ggtgtaatgc
     6001 catgatgagg cataccttcc ggtatttggt catcattgcc gccgcaggat tacttttcct
     6061 atgcgcatgc agtcctcagg atcagaccgg tgaaaacacc tcccatgcgt cttcagcctc
     6121 ggctcagcag gaggaaacaa atacctcttc aaaggaagag acggcagcaa cctccgaaga
     6181 atccgctgcg gaagttgagt gcaactggac ccttgaagtc agcgatacag taacgacaca
     6241 gcttaacggc tacgacttta aatgcaccct tgcggttatg gcagtcaagc tgggcggtaa
     6301 ggatgaactg ggaacctaca ggggaattgt cactcttaaa tatcaatatg acatgaagca
     6361 gggcaatgtt gccggcaacg ccgcaggtga gggtcaggaa atagatgccg tcattgaggt
     6421 cgtacaatac gaggaacagg cgtacaatga ggcaacaggg cagacagttc tggcagaact
     6481 tgttcaatat gacgcaatgg ccataggcag cctggttttg accggaagcg gtgaagccgc
     6541 cgaatctgcc ggaggcgcca gctggagcac aagtgacagg aagacaatcc cggtgccgta
     6601 taggatggca gtggacggcg gcaaggtaac cattgagctt tatactattg ctccgggagt
     6661 caaattcaac ggaatgatta caggaactcc aatttgagga agtaccgatg ataataagaa
     6721 agccagccac caaatcgtga ctggctttct gaatttattt atcatattaa ttaccgcttg
     6781 cagctgcttc ttttacacag gcttgatgct tattgtccac tttgtttgta ttgtgggtcc
     6841 tgctgttaag gaaatgaaca tctactacac cgctcatccc attgttcttt atcttgtcaa
     6901 gatttgcacc tctgccgtaa ccgccgctcc tgctgctgat atacttatct gcgggggact
     6961 tgtccactcc tgcatgaggc atagccgcca ttgatgccgc tatcttccgc ccgtcaactt
     7021 ccacaactat cggccttcgt tcccagctcc agccgcccca aatctctttg atctttttgg
     7081 aatcctccgc ggtcctggcc tcacagtcag cgtggttgcc gccgtatgta cggacaattt
     7141 caaaggtttc tcccgttctt acgtcggtta cctttgccac cgttcctctt tcaaaaattt
     7201 tggatgcttc agaccaccaa tccagagcct ctacttcagc agtatatgtc cattcagttc
     7261 caaaaagcac tttccctgta tccgggccta caatcccatc aacagtcaga ttgttatcct
     7321 cctggaactt tctcaccgct tcttcagtaa cgtttccgaa gtaacctgtt acttcctcgg
     7381 taaaataccc tttctccttc agctgagact gaatcctgga tacttcctcg cccctgcacc
     7441 cgttttttag aacatcggca aaagtaacag aagtagttaa aagtatcact gcaagtataa
     7501 ataccaatga tttgccaaac ttcaacactg tctccccttt ctgcttacgg acttcactta
     7561 acccgttttg tccctcgctc ttctgaggaa ttcagccaat tatatagctt gtctacattt
     7621 tagcaatttt ttattaccaa ttcaatggta aaaaaataca gtgttatacc ataaatgcac
     7681 ctaaagtccc tgccatttca ggttgggctg ataattcagt cgtttgtttt attgatattt
     7741 caataagcaa agcccaaagg agaataatcg atacaaataa tcccgtatga ctgcctataa
     7801 gcttcaggct attcgcaaag gtagtcaccc ccggggctat ctgctgctct tcggcttcca
     7861 gcctggcagc ctttattgta agcagactgg ctataacact taatactgat gctaatacaa
     7921 gaaaggtatc ctcttcaatc acatttacaa taatggagct tgtaaggagc attctgccgg
     7981 ccagctttgc ttcagcagta cccgcccttt cccgcccctc ctgcggcagt acgatttctg
     8041 tatccatatc caactcctcc ccagtaccat tatattattg tacatgttct gtgtgcttct
     8101 tgaatcctcc attagggctg ctgcacaata gaagaatgat gcgacacaac tataagtttg
     8161 cagtcatact cccagagcac aagccttgta tcccgactcg cacgatatta gctttggaca
     8221 gaatcagagc ttgatacttg taaaatcctg acgcacataa acccaacctc ctgttggtat
     8281 gtgctggatt tacctcacgt aaatcccacg gattttactg cgcatcttct ctcgattctg
     8341 ttgcccaaag ccaataagtg ctcgctgtta tataaggcta gtgctctgca ggataactac
     8401 aaacttcaga tacaacttac acggattatg attttaaagt tactagtgtg ccctgctata
     8461 ctagacaagc tcttgcttgg ttatacattc ttcagaatac tgccttgtag ataaaggata
     8521 tgcaaatacc gacgtgggga agatatcttc gacgaaacct tccacctgac cccggaacgg
     8581 gaatacctcc aggcaaaata accagacggg cccgcagaga tgggaccttc gccagtgatt
     8641 ttgctggctt tggaaggtga ggagaacata cttcccctac agtcggagta cttcacaaac
     8701 ctgtatatat tgcgcattat tcttcttata tgtctttcag gtttagtact cttatggcat
     8761 tcaataccgc tatcagcgca actcccacat cagcaaatac cgcttcccac attgttgcca
     8821 atcctccggc acccagaagc agaacaatca gcttaatact cattgcaaat acaatattct
     8881 gcagtactat cctcctggtt ttctttgcta tccttatagc gctgacaatt ttggagggct
     8941 catccgtcat tatcactata tccgctgctt caattgccgc gtcggagccc aggccgccca
     9001 ttgcaattcc gatatctgcc ctggccaata caggggcatc gttaataccg tctcccacaa
     9061 aggcaacctt gcccccggac ctcatctcat tcttaatcca ttcaagcttt tcaacctttt
     9121 gatccggcaa taactccgaa tacacctcgt ccagtcccag cgccgaccct gtcttctctc
     9181 ctggcgccct ggcatctccg gtaagcatta ttgtttttct tatgcctgaa gccttaagga
     9241 ggagaatagc tcttgccgca tcatctttta tctcatcaga aattactata tatccggcat
     9301 aagctccatc tactgctaca tgcacaagcg ttcctgcaat atcagggttt tctgctgata
     9361 ttccctccaa ttgcatcatt tttatatttc ctgctgctac ccttttcccg aaggcctctg
     9421 ccctgacccc atggccggaa atctcctcgt aattcgaaat ttcactgccg attatctcac
     9481 tgccgtatgc cttaagtatt gataatgcaa tgggatggcc ggagtagctt tcgatatatg
     9541 ccgcatattg caatagctct ccttccttca tttcacccag tgccttgatt tcagtcactt
     9601 taaagactcc ttttgtcagt gtgccggtct tgtcaaatac aatggtatcc acctggttta
     9661 atgcatcaag gtagttactt ccctttataa gcactccgtt tttcgaagca cctccaatac
     9721 ctccgaaaaa ccccaaaggt atggatatga ccaaagcaca gggacatgaa accacaagaa
     9781 atatcaatgc cctgtaaatc cagtctgaaa aagcttctcc ctgcagcacc aaaggcggaa
     9841 caaatgcaag agctaatgcg gaaaacacca ctgcaggagt gtaatacctt gcaaacttgg
     9901 taatgaagtt ttcggtgggg gattttttac tacttgcgtt ctctaccatg tcaagaatct
     9961 tggataccgt cgattctcca aactccttgc tcacttcaac ggtaagaaca ccgctgttat
    10021 ttataaagcc tgccagaatc tcatccccgg cattggcctc ccttggcact gactctccgg
    10081 tcaaagccga agtatccaac attgatctgc cttttgttat tctgccgtcc agaggaaccc
    10141 tttcccccgg ttttacaatt atataagctc ccaccggcac atcttccggg ggcacctgta
    10201 tcatctggcc ctcagagacc agattggcat aatcaggccg tatgtccatc agttctttta
    10261 tcgaccttct ggaacgatct acagctaaat cctggaacat ctcccctatc tgataaaaaa
    10321 tcattaccgc aacgccttcg gagtattctc ctattgcaaa tgctccaata gttgcaagac
    10381 tcataaggaa gttctcatca aaaacttgcc ccctgaaaat attccttatc gatttgacaa
    10441 gtacttctcc tccgaccaat agatatgctg tcagatacat agccaattcc acgcctgcag
    10501 aaagcttaag caaaacagcc gcagcaaaaa atgcggcacc aataccgaat tgaaacggct
    10561 tcagcctttt tatccctgat tcattttttt cttctgaatc tgcttcccag gcctttctgt
    10621 ctgttatctc tttcagcttt acatcaggct taatattcct tacgatttgc tttacttcct
    10681 ttattgctgc cgccgcatcg gcagattcga atatctcaag cttcagcttt tttgtcacaa
    10741 aatctataga tgaattccta atcccttcca gggatttgat gccaagatcc atcttgtcag
    10801 cacaaactgc tcagccaagg cctgtaagta tgaattcttt tacaacatat tcatcgtttt
    10861 tttcaaaatc tgctgctgct tctcttttca cattaatacc cccgtctcac gctttgttta
    10921 atgctatctc tccctgatat gcaccagtcc ttggtcgaat atctgtttta catgttcatc
    10981 atccaaggaa taatatacta ctttgccttc ttttctgtat ttcaccagcc ttgcctgctt
    11041 caaaaccctg agttggtggg atattgcaga ctgattcatg gataacagtg ccgctatatc
    11101 acatacacac atctcagatt caaacaacgc gcacaatatt ttgattcttg ttgtgtcgcc
    11161 aaaaaccttg aacaactcag ccaggtcata gagcgtctct tcttcaggca tactgttcct
    11221 tacctttgac acagcatctt catgtatcat ggaacagctg cagctttcaa tacttaccct
    11281 gttttcttcc atagcgccct ccatattatt atatatgaac atatgtttat gtgttcatat
    11341 gttcatatat atattactgc tttaattcat aaaagtcaac aacaaagagg gacatgggga
    11401 cgttcgggac atggggacgt ttctcctgtc ccgctgaaag cgggacagga gaaacgtccc
    11461 catgtcctca aaaaacaatt gtaaccaaaa gcagattaca acataaaata tgaataggat
    11521 gatgaacaac ctcttctgca gaaggaggtg taattatggc gagaggtttt aaccatggtt
    11581 tgtatggatt gaataagccc ggctgagagc cgggttcagc accggaagat ttttcgggag
    11641 tattttactc aaaatcaaga aagcagcaac tctctgcggc agttcccccg ccacccccgg
    11701 aaccggcccc ccctgcagca attcccccgc ctcaaaatct gggcggaatg tttaacgggc
    11761 ttttgggaaa tgtgtttgag aataataatt ggtggttgtg ggctgcttta ctttttatct
    11821 tctttaatcc cgacctgaga aaaaaactca taggaaagtc aatagaggct gaccgggacc
    11881 cttagtcagc ctctctcaat cccaagcctt ttctcttgga aatgatatgc gcttctatat
    11941 tgtttgccgc ttccgccgga tcatccccca gtgcaatctt tccccctgtc agtccttcca
    12001 catcttccgt aagaagctta accaaccccg gagcccccgt gacgaatggt attggcgaaa
    12061 ggcgggtata ggcaccgtag gctgctgcaa agaagccgtc gatggtagcc ttctgctcca
    12121 tccattcagg tgccgttacc gctatgggaa gctgcggtat atcgacatcc agatggtctg
    12181 caatttcacc tgccattttg ttcacatcct gcttgctgtc aatccctacc gtatcgcaca
    12241 tccatttaag cttatttgca tcggtaatct taaagggagt tttcccctgg gcggtctccc
    12301 tcagggttcg gaaagcctca taggcatgat ggctgtacgt acctgccccc atgatgtttc
    12361 tcagaagcat attcctcatt gccattgtgt caccgcctat tccgcataca cccttatcct
    12421 gtccgctttt ttcactgatt ctgcaagggc cgttggtaca aagctggcag cttaatccct
    12481 gaaggcaaaa tttacagcgg atcttttcct gctgtgcata tctgtcaaag gcattggaca
    12541 ttccgtcttc ccttattcga ggaagcatct cctctcccga atcatgatag ctgacccgtc
    12601 cttcagtctt ttctgaaata gtttctccca ttgataaatt ccttcctttc aagcatgttt
    12661 cagagtaagt tttctcataa agcagggtaa atatgtgctt aagcactgta taccgaataa
    12721 aaaaagcatg atacttattt aagcatcatg cccatttttt attctcctga ctccaactca
    12781 taaggccaat ccattattcc gcccatattg tataccttgc tgtatccaag ccctgccaat
    12841 atgttagcag cactgctgct tcttttgccg cttgcacagt atacgataat atccgcatcc
    12901 ttatcagtca gctctgcttc cgctctcttc tccaaatcat taaccggtat taaaatactt
    12961 cccggtatat gttgttcttc atattccccc tgggtcctta catctaacag tataatatcc
    13021 tcttccgatt ccagcctttt gtatgcctct tcaggagaaa tgctttcggg ctgaactact
    13081 tctgcaggac tttgttcggg ttgaactatt tctgcgggac tttgagagat gtcctgctgc
    13141 ggttttgtac tgcaggctga aaatactgcg gcccccaata atactgccag caataatatt
    13201 actattctct tcattcgctc caccgtcctg ttacaggctt cccttcaaac caatttcttc
    13261 tgcagctttc ttcattccat taattgtttc tccgataata gtgtccattt ccatgcccag
    13321 aagcgctgct ccctcttcta taacctccct gttgacgcct gccgcaaagc tcttttgctt
    13381 ccatttcttt tttacggatt ttacttccat atccaatatg cttctgcttg gcctcatgat
    13441 ggcagtcgct gcaataagcc ctgtcagctc atcaatagta taaagtattt tttccatttt
    13501 ctctgtcggt tctacgtcgg agcaaagctt ccaaccgtgg ctcagcactg catgaatata
    13561 atcttcaggc catcctcttt cggtaagtat ctcttttgct tttatacaat gctgttccgg
    13621 gtacatttca taatcaatat catgcagcag gcctataatg ccccattttt cctcatcctc
    13681 cccaaggagt ccggcaaaat gcctcataac ggattcaact gtcagtgcat gcttcaccag
    13741 gctgtcattc ttgttatact cctgcaggag ttccaatgcc gctgctctgc tcggaattgt
    13801 gtttaccata gaaaatcctt ctttcgtatt gtttttttgt ttgcattacc tgccggcttt
    13861 tttctttagt acggccttgg tgcttttcgc tatatcggct gccgtcaggc catacatttg
    13921 gagtaaaata tccggtttgc cggactttcc gaacttgtcc tgcacaccaa cccttacaag
    13981 gggtacaggg taattctccg aaagtacttc agccactgca cttccaaggc ccccgataat
    14041 attgtgctct tcagcagtga cgatagcccc tgtttccctg gcagctttca ctattatatc
    14101 cgcatcaata ggctttatgg tatgaatatc tattaccctt gcatttattc cttcctgctg
    14161 aagcagttcc ttcgcttcaa gagccgcact caccataagc ccggtagcaa taattgcagc
    14221 gtcatttcct tctgcaagta caataccctt gcctatttcg aacttatagg agctttcatt
    14281 atatattaca ggaacgttca atcttcccaa tctgagatat acaggcccct catgctctat
    14341 tgcggccttt acggcatatt tggtttcaac agcgtcagca gggcttatta ccgtcatatt
    14401 gggaagcgcc ctcatgattg caatatcttc aattgcctga tgcgatgccc cgtcttcccc
    14461 tactgataaa ccggcatggg ttgcaccgat ttttacattt aattttggat agcatattga
    14521 attccttatc tgctcaaagg ccctccctgc ggcaaacatg gcgaaggtac ttgcaaatac
    14581 caccttgccg caggaggcca ttcctgcagc cgtagccatc atattgcctt cagctatgcc
    14641 catattgaaa aacctttcag ggtatttttt tctgaaatta tctgtctttg tggattttga
    14701 caagtctgca tcaaacacca ctattctgtt gtcggagcca aactccgcaa gtgcatttcc
    14761 gtatgcttcc cttgtagcta tcttatctgc catactattc aacctccaac tctgccagta
    14821 ctttatccag ttccgccatt gcctggtctc tttgttcctt gtttggagca ctgccatgcc
    14881 agcctgcctg gttctccata taagaaaccc ctttcccttt gacggtctcg gcaattatta
    14941 ttgtaggttt gcctttacaa tccttggcct gccctataga ctcaagtatt ttttcaatat
    15001 catggccatc ggtcttaagc acattccaac caaaggcttt aaatttatcc gctacaggtt
    15061 caggagacat tacatcggta atcctgccgt caatctgaag cccgttatgg tccagaaaag
    15121 ccgtcaggtt atcaagcttg tagtgagccg cagccataaa agcttcccag acctcgcctt
    15181 cctgaagctc tccgtcaccc agtacggcat agaccctgca gtccttctta tccagcttgc
    15241 cggccatagc catgcccaca gctgccgaaa tgccctggcc caaagacccg gtggacatat
    15301 ccaccccggg tacatatctc atgctggggt gcccttccag atagctgtca gctttcctga
    15361 aggtcaaaag gtcctcttta gggaaaaagc ccttttctgc cagtaccgca tataaggcag
    15421 gggagcaatg gccttttgac agtacgaacc tgtccctgtc ctcccataag ggctgtttag
    15481 tgtttacacg catttcagcg aaatacaata ctgttaaaat ttcggtacaa gacagggatc
    15541 ctcccggatg tcccgatgca gcgctgtaaa cttcgtctat gatgtgcttt cttactttga
    15601 ctgcatactt ttttagatct ttaatttcag gttttttcat ttgctgcctc ctagtatttt
    15661 ataaaattat agcttccata tatttaagcc gcaatagtca tcaaataccc ttcctatttc
    15721 tccatcaact gcatctataa taagctcaca ggtcccggaa atccagcact gattcaaatg
    15781 ccccccatga gcattatatt cctcatcgcc caatgttata tgcagatgaa ggtatacttc
    15841 accatctttt tcagttatgc ttcctgtcag attagtgatc tcatattctc cggtaagctc
    15901 ttgattatga tattccttcg taaccgtgtt gaagacacct atcgtaatcc tattagctgc
    15961 cccgatacca atgacagacc ccaacttcaa accgttatcc gtgcaaaatt tatataatga
    16021 cgcaactaac tcttcaccct tatcaattct cacaaaatac ttactgccta tttttgtatg
    16081 cttcatattc tcaccctttc caaatagatg catcgaactt ttgaacttat tatgacccaa
    16141 atattctttt atgtcaatat taccgaaata gggagtattt atctctccgt ccccccgggc
    16201 attctccatt ttctctacaa aaatccgctt ctttagtgta taatgatata gtgattttaa
    16261 tatttggagg atactatgat aaagattgat aatgatacgg taagatttga aggctacgaa
    16321 ttggaaaacc ttgtggtttt attggaacag gtagtaagaa attcgattat agattccgac
    16381 agcagggaat tcgcgatctc cttcttaaaa gctttcagcg attgcttcgg tgaagatgtt
    16441 attaagaagt tcaattcaat cagaaaatag aattctcata tatgaagaat aatgcgacgg
    16501 ggacatgggg acgtggggac acggggacgt ttcgcgcggg gacacgggga cgtttcgcgc
    16561 ggggacacgg ggacgtttcc cctgtcccgc tttcagtggg acacgcgaaa cgtccccgtg
    16621 tccccgcgtc cccgtgtcgc attattctat ttttatgcga
//
LOCUS       DAOOAB010000055        16650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_4534_length_16650_cov_16.346128, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000055 DAOOAB010000000
VERSION     DAOOAB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16650
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4534_length_16650_cov_16.346128"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1414)
                     /gene="purD"
                     /locus_tag="PKU88_10495"
     CDS             complement(152..1414)
                     /gene="purD"
                     /locus_tag="PKU88_10495"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPX69742.1"
                     /translation="MKVLVVGSGGREHTLVWKIAQSKLADKVYCAPGNGGISELAECV
                     DIKADDIDGLLEFAKTEGIGLTVVGPEVPLSLGIVDCFEENGMRIFGPCRKGAMLESS
                     KAFSKDFMIRNKIPTAAYKVYYEAEKAKAGLDDFGFPVVIKADGLAAGKGVIIAQNRE
                     EAISAIDDMLMVRKFGEAGSKIVIEEFLVGKEASILAFVDGKTAVPMVSAQDYKRALD
                     NDEGLNTGGMGAVSPAFYYDEKTAAVVERDIIRRTVDALKAEGICYKGVLYFGIMITE
                     KGPKVLEYNARFGDPETEVVLLRLESDLVEIMNSVIDEKLEAQKIQWKDEQAVCVVMA
                     SGGYPEHYEKGYEIKGLDEAAEIDGVTVFHAGTKKASGKTVTAGGRVLVISAAAKNYE
                     EARDKAYSAISSIAFEKAHYRKDIGKKD"
     gene            complement(1428..2969)
                     /gene="purH"
                     /locus_tag="PKU88_10500"
     CDS             complement(1428..2969)
                     /gene="purH"
                     /locus_tag="PKU88_10500"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPX69743.1"
                     /translation="MIKRALISVSDKTGIVDLARELAKQGVEIISTGGTAKALEEAGI
                     KVIGISDITGYPECLDGRVKTLHPNIHGGLLAIRDKAEHMDKLRELGIGTIDMVVVNL
                     YPFRKTIEKEGTELPEAIENIDIGGPSMLRAAAKNYKYVTVVVDPKDYSKVLSEINNS
                     GDTTEATRFELAAKVFSHTASYDALIGNYLWSKAGLPKYPETVTMTFEKVQDLRYGEN
                     PHQGAAYYKEMKKVSGSIAAAVQLHGKELSFNNINDANAALETLKEFDEPAVVALKHA
                     NPCGVGVGSSIYEAYMKAYEGDPVSIFGGIVALNREVDEKTASEIGKIFVEIVIAPSY
                     SEEAMNVLTGKKNIRIMKLEDISRKPEADIDVKKVGGGLLIQDADWADFNMADINYIT
                     DRKPTEKEMQDMLFAWKVVKHVKSNAIVVAKDGMTLGIGPGQTNRIWSANMSLERSGE
                     KAKGAIIASDAFFPFGDVVESAAKAGITAIIQPGGSLRDQESIDGCNKHGIAMIFTGV
                     RHFKH"
     gene            complement(2977..3594)
                     /gene="purN"
                     /locus_tag="PKU88_10505"
     CDS             complement(2977..3594)
                     /gene="purN"
                     /locus_tag="PKU88_10505"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPX69744.1"
                     /translation="MRKVRIAVLVSGGGTNLQTLIDAVDKGDINGEIAAVISDNENAY
                     ALERARKHGIKAIYINRKLLAERLMAELQKLDIELVVLAGFLSILDRELVKAYEGRII
                     NIHPSLIPSFCGKGFYGERVHKAALEYGVKVSGATVHFVDEGTDSGPIIFQEAVPVYF
                     EDTSETLAARVLQVEHRLLPAAVGLFCEGRLRIEGRKVKILKEEV"
     gene            complement(3582..4622)
                     /gene="purM"
                     /locus_tag="PKU88_10510"
     CDS             complement(3582..4622)
                     /gene="purM"
                     /locus_tag="PKU88_10510"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPX69745.1"
                     /translation="MDEKLTYKAAGVDVEAGYEAVKLMKQHTKRTMIPGVLGELGSFG
                     GFFEIDRESYKNPVLVSGTDGVGTKLQIAFMTGKHDTIGIDCVAMCVNDVACHGAKPL
                     FFLDYIGTGKLMPNIAADIVKGVCDGCVEAGCALIGGETAEMPGFYKDGEYDLAGFAV
                     GIVDKGNVINGSNVKEGDVLIGIASSGIHSNGYSLVRKIFFGINNFDINTRFDELEGT
                     LGEELLKPTKIYVRTVQSLIVKHRINGIAHITGGGFIENIPRTLPAGLKAQIDLGSWY
                     IPPVFRLMQRLGSIEEKEMFNTFNMGIGLVLAVGKQDAEGILGTLEELGEKACIIGSV
                     AGGEGGIEFCGR"
     gene            complement(4648..6048)
                     /gene="purF"
                     /locus_tag="PKU88_10515"
     CDS             complement(4648..6048)
                     /gene="purF"
                     /locus_tag="PKU88_10515"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPX69746.1"
                     /translation="MDKLKEECGIIGIYDKGNMEIESLTYYGLIALQHRGQESAGIAV
                     NRDGVITCYKEMGLVSEVFDDKILNLLKGDISIGHVRYSTTGDSFAHNAQPLLVKYKK
                     GSIALAHNGNLVNAGSVREELEDQGMIFTTTIDSEVIAGLIARSCSKGIEDAILDTMD
                     IIRGSYSLVIMTDDKLIGVRDSHGLRPLVIGRIGNGYVLASETCALDTIGASFIRDVR
                     PGEIIIIDENGMRSIQCRSTGKQAGCIFEYIYFARPDSVMDGISVYQAREKAGRILAE
                     EQPADADVVISVPDSGTPAAIGYSLQSGIPYAIGLIKNRYIGRTFIQPSQVTREIGVA
                     LKLNPLKANIEGKRVVMVDDSIVRGTTSRKIVQALKAAGAKEVHVRVSSPVITHSCHF
                     GIETSNRDELVGATSVLEAIREKIGADSLGYLSYNGILRSVGSTGEGFCTACFSGRYP
                     MDIPENCSKDVFERKK"
     gene            complement(6059..6763)
                     /locus_tag="PKU88_10520"
     CDS             complement(6059..6763)
                     /locus_tag="PKU88_10520"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPX69747.1"
                     /translation="MKRLGMIYEGKAKKVYKTDDEALYIVEYKDDATAFNGLKKGTII
                     NKGVMNNRISEKLFRLLEEKGIDTHQVERISDREVVVKKVKIIPLEVLARNYAAGSLS
                     KRLGLEEGVKLNSTVLEFSYKNDELGDPMVNDYHIKAIGIATEQEVAKITEMALKINE
                     ILSEFMLTKGILLVDFKLEFGKTPDGKIILADEISPDTCRLWDAVTLEKMDKDRFRRD
                     MGKVEEGYMEVVKRLC"
     gene            complement(6763..7272)
                     /gene="purE"
                     /locus_tag="PKU88_10525"
     CDS             complement(6763..7272)
                     /gene="purE"
                     /locus_tag="PKU88_10525"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPX69748.1"
                     /translation="MLVSSDKPKAAVIMGSDSDLSVVRKAIEILKAFEVDYEVNVISA
                     HRTPDRAAEYAKKAEENGFEVIIAAAGKAAHLPGVLAAFTTLPVIGVPIKSSVLDGLD
                     SLLSIVQMPSGIPVATVAVDGAENAGLLAVQILSVKYGNLKDKLKAYKEKMKQDVIKK
                     DEKIKEEVF"
     gene            complement(7300..11073)
                     /locus_tag="PKU88_10530"
     CDS             complement(7300..11073)
                     /locus_tag="PKU88_10530"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPX69749.1"
                     /translation="MNGNVRRIFVEKKDDYAVSAKDLFNDIRENLGIKSLTNLRIVNR
                     YDISGITDSEYNSAKNTIFSEPPVDNSYDEKIIIDKNDRLFAIEYLPGQFDQRADSAA
                     QCLQILTQKERPKVVTAKLIILSGEISDDEFEKIKNYCINPLELREASLEKPESLEAV
                     YPEPGDIKEVKDFITMDEAGLARLMEVMGFAMSSEDLRFCREYFKTSEKRNPTVTELR
                     VIDTYWSDHCRHTTFNTLIEKIEIEKGKYTEAIEKALLRYMDARAFVYGDSERDITLM
                     DMAVMGMKEMRKKGLLDDLEVSDEINACSIIVNADIDGKREEWLVMFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRSYVYGAMRVTGSADPRASIEETLPGKLPQRKITTGAA
                     AGYSSYGNQIGLATGQVAEVYDEDFVAKRMEIGAVIGAAPKSNVIRKAPSEGDVIILL
                     GGRTGRDGCGGATGSSKEHTEESIQSCGAEVQKGNAPTERKIQRLFRDPMVSRMIKKC
                     NDFGAGGVSVAIGELADGLLIDLDAVPKKYEGLDGTELAISESQERMAVLLAPEDAES
                     FINYAAAENLEAVVVAKVTGDNRLRMQWRGQTIVDISRDFLNTNGVRQSTRVLIKSPD
                     AESCFNKLQAWEKSKEDLKGKWLENLKDLNVCSQKGLVERFDSTIGAGTILMPFGGIY
                     QETPSEGLAMKLPVLSGETNTATVMTYGYNPKLAKWSPFHGAVYAVVEAVAKVAALGG
                     DYGRIRLTLQEYFEKLGKVPEKWGKPAGALLGAFYAQMMLGVPAIGGKDSMSGTFKDL
                     NVPPTLVAFAVDTAEAGRIVSQEFKKSGSKVILLRAERDDKDLPDFEKLHKIFSKVHE
                     LIQKGLVLSSATVRIGGIAAAVSKMCFGNKLGFAFEKGYEERDLFTPEYGSIILEMDQ
                     SCDLNGVFSGMDYEIIGVTESTGYISIKGIKIELEEALKAWKAPLERIFPTHSGDNDD
                     KPQNIYYNSTNIVKSSAKIARPKVFIPVFPGTNCEYDTARAFEKAGGEPSIFVFRNIG
                     PSDIEASINEMVRLIGNTQIIALPGGFSSGDEPDGSGKFIATVFRNPRVKEAVMKFLR
                     EKDGLMLGICNGFQVLIKLGLLPYGEIMDMDESSPTLTFNKIGRHVSCMVRTKVVSSL
                     SPWLSKSKVGDIHTIAVSHGEGRFAASREIMDSLIKNGQVATQYVDLNGNPTYDSAFN
                     PNGSIEAVEGITSPDGRIFGKMGHSERTGANVGKNVPRDKEQLIFKAGVEYFS"
     gene            11258..12151
                     /locus_tag="PKU88_10535"
     CDS             11258..12151
                     /locus_tag="PKU88_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPX69750.1"
                     /translation="MDINFELYKVFYYVSKSLSFSEASEELFITQSAVSQSIKLLEEK
                     LKCKLFFRNTKRVKLTREGEILYSHIEQAFNFIKSGERILEERNSLTQGEIRIGTSDT
                     ICKYYLMPFIKSFNQAYPNIKIHLTNRTSPRCIELLRKGSVDLSVINIPEKLNYSNII
                     VKNTKRVRDVFIANENFKELKGRKVQLKELEEYPLLVLERNSTTREFFDSLMAKHKVS
                     IKPEIELGSIDILMEMSKIGLGIAFVPDECVRIGLAKDDVFIIDIKEEIPERNLGFVI
                     HSNIPLPVAASKFIELLEGEH"
     gene            complement(12116..13168)
                     /gene="fni"
                     /locus_tag="PKU88_10540"
     CDS             complement(12116..13168)
                     /gene="fni"
                     /locus_tag="PKU88_10540"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPX69751.1"
                     /translation="MEDQKSAREKRKDEHLKYFLLSKPSGYNGFSDVVLQNNSLPDAD
                     FDEISTECVFLNKYIDSPLMINAMTGGTEYSGDINRKLARLAVKFNIPIAVGSQTVAF
                     DNPKAADTFKRVRDIHRDGIVIANLGANRDIEDVYKAIDMIDADAVQLHINIVQEMCM
                     AEGDRNFRGVLENIKKIAALAEVPVIVKETGFGISFETAGKLQAAGVKYIDIGGKGGT
                     DFVSIESLRNSDRECPFLENWGIPTALSLFQCRKASSDMFIICSGGISKAEEVVKALC
                     MDGDMAALGGTILRILLEQGYDAAEKYMAALLYEIRVLMLLLGAKDITDLKNVSYLLK
                     GELGELVLSLKKLYKF"
     gene            complement(13267..13746)
                     /locus_tag="PKU88_10545"
     CDS             complement(13267..13746)
                     /locus_tag="PKU88_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPX69752.1"
                     /translation="MAVLIFYPAVGSKSLQLTWSNLLEMMAILPPIFILLGLLDVWVK
                     RETMIKYMGDDSGIIGVLLAFFLGSAAAGPLYAAFPVAGVLLKKGSKLSNVFIMLGAW
                     STTKIPLILFEASSLGLKFMLVRMVMDLIGIAVIAYFTERMLSKGEIEAIYQNASNR"
     gene            complement(13809..14282)
                     /locus_tag="PKU88_10550"
     CDS             complement(13809..14282)
                     /locus_tag="PKU88_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPX69753.1"
                     /translation="MTASLYVIAILALSVSLLKSKERTILALKKAWKSFENILPQFLS
                     ILIIIGILLAVLSPEQISKLLGRESGWYGVLIAAVIGSVTLVPGFIAFPLAAALLKSG
                     AGYMQIAAFISALMMVGIVTIPVEVEYFGKRAAAVRNTAAFVFSLIVALVMGVVM"
     gene            complement(14299..14658)
                     /locus_tag="PKU88_10555"
     CDS             complement(14299..14658)
                     /locus_tag="PKU88_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPX69754.1"
                     /translation="MIKIAVASENDMVTEHFGHCMNFNIFEAENDRIVKSESILNPGH
                     RPGFLPNFLNNMGVNVIISGGMGGGAIDIFNEKGIEVVLGARGYAKEAAEAYLKGTLK
                     STGSVCHEHQHHDECGK"
     gene            complement(14655..15494)
                     /locus_tag="PKU88_10560"
     CDS             complement(14655..15494)
                     /locus_tag="PKU88_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPX69755.1"
                     /translation="MSEQCNQNCSSCSESCTERKEEFDFSEKPHALSSIKKVIGVVSG
                     KGGVGKSLVTSMLAVTMNRMGYHSAVLDADITGPSIPKAFGIREKARGSEKGLYPIKS
                     HTGIDIMSINLLMDNDKDPVVWRGPILANTVKQFWSEVIWSDVDFMFIDMPPGTGDVP
                     LTVFQSIAMDGIIIVTSPQELVSMIVSKAVRMAEIMNIPIIGLVENMSYFKCPDNDKE
                     YKIFGDSHIDEIAEEHNLKVLAKLPIDPRLSAACDKGMIELFEGDWMEPVGKILENME
                     DKK"
     gene            complement(15519..16394)
                     /locus_tag="PKU88_10565"
     CDS             complement(15519..16394)
                     /locus_tag="PKU88_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX69756.1"
                     /translation="MKQLLILSGKGGTGKTTIAGAFIKLSHAKAYADCDVDAPNLHLI
                     MGRNSEPEKRDFYGLPKAEINQELCIRCDRCRQNCRFEAILSDKAYKVDYFACEGCGV
                     CEAVCPAGAVSLNDAVAGELMLYSDEDKVFSTAQLKMGSGTSGLLVTEVKKQMRLNAK
                     DPELAIIDGSPGIGCPVIASLSGADMVLIVAEPSLSGISDMERIEKTAATFQTKAAVC
                     VNKYDANIANTARIEEYCRTNNLPFVGRIPFDPEAVKAINEGLTVIDIDCPAGNAVRE
                     VFNKTTKLLFDTSTI"
     gene            complement(16372..>16650)
                     /locus_tag="PKU88_10570"
     CDS             complement(16372..>16650)
                     /locus_tag="PKU88_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPX69757.1"
                     /translation="HNLNMVYELVKLFRKPYGVVLNKCQEGENPAERFCEEKGIRILG
                     RIPFDNELGTLNSNAKIAVRESEKYRTVFSSLLETVTKEVRHEAAVNP"
BASE COUNT         4035 a         4475 c         3094 g         5046 t
ORIGIN      
        1 cctttgacag cgttaggttt ttcttagttg agtggaatta gagccatcaa tttttgtata
       61 cctatgcgag actatacatt gctgttctta tatcggaggt ttttcatcag tctcaaagcc
      121 ccttacggga ctttgcaata agttcaatga tttaatcttt tttccctatg tctttcctgt
      181 aatgggcttt ttcaaaagct atactgctaa ttgcgctata tgctttatct cttgcttctt
      241 cataattttt tgcagcagcg cttattacaa gcacccggcc tccggcagta acagtttttc
      301 cggaagcctt ttttgttccc gcatggaaaa ccgttactcc gtcaatttca gcggcttcat
      361 ccaggccttt tatttcatag cccttttcgt aatgctccgg atacccgccg gatgccatta
      421 caacacagac tgcctgctca tctttccatt ggattttctg agcttcaagc ttttcgtcta
      481 ttaccgaatt cattatttca accaagtccg actcaagcct cagaagcacc acctctgtct
      541 cgggatcccc gaacctggcg ttgtattcca ataccttggg gcccttctcg gttatcatga
      601 ttccgaagta taatactcct ttgtaacaga tgccttctgc ctttaaagca tctacagtac
      661 gccggattat atccctttcc actacagcag ccgttttttc atcataatag aatgcgggag
      721 atactgcgcc cattcctcct gtgttcagcc cttcgtcatt gtccaatgcc cttttgtaat
      781 cctgggcgct taccatgggc actgcagttt ttccgtcaac aaaagcaagt atcgaagctt
      841 ctttgccaac aagaaactcc tcgatgacaa tctttgagcc tgcttcgcca aacttcctga
      901 ccatgagcat atcatcaatg gcggagattg cttcttctct gttctgggct attattacac
      961 cctttcctgc cgcaagcccg tcagccttga taacaacagg aaaaccaaaa tcatcaagcc
     1021 ctgccttggc tttttcagct tcataatata ccttataagc tgcggtaggt attttgtttc
     1081 ttatcataaa gtcctttgaa aaggccttac tgctttcaag cattgcgccc ttccggcaag
     1141 gcccgaatat tctcatgcca ttttcctcaa agcaatctac aatacccagg ctcaaaggca
     1201 cctcgggacc aaccaccgta agcccgatcc cttcggtttt ggcaaattcc aaaagtccgt
     1261 caatatcgtc ggcctttatg tccacgcatt cggccagttc actgattccg ccgttccccg
     1321 gcgcgcagta cactttatcc gccagcttgc tctgagctat cttccataca agggtatgtt
     1381 cccttccgcc gcttccgacc acaagtacct tcatattatc acctcttcta atgcttaaaa
     1441 tgccgcaccc cagtaaaaat catcgcaatt ccatgcttgt tgcatccgtc tatggactcc
     1501 tgatccctta aggagcctcc cggctggata attgccgtta ttcctgcctt tgcggcggat
     1561 tccaccacat ccccgaaggg gaagaatgca tctgaggcta ttatagctcc ttttgccttt
     1621 tccccggaac gctcaagact catatttgca gaccatatcc tgttggtctg gccgggtcct
     1681 atgccaagag tcataccgtc tttggccact acaatggcat tggacttcac atgctttacc
     1741 accttccatg cgaaaagcat atcctgcatt tccttctcgg taggttttct atcggtaata
     1801 taatttatat cagccatatt aaaatccgcc caatccgcat cctgaataag caggccgcct
     1861 cctaccttct tcacatctat gtctgcctcc ggcttgcggc ttatatcctc aagtttcatt
     1921 attctgatgt tcttcttgcc ggtaaggaca ttcatggctt cttccgaata ggagggtgca
     1981 atcactattt ccacaaatat ctttccgatt tccgacgccg tcttttcatc cacttccctg
     2041 ttaagggcta ctataccgcc gaatatggat acgggatccc cttcataagc tttcatatat
     2101 gcctcatata tgctgcttcc gacacctact ccgcagggat ttgcatgctt tagtgcaaca
     2161 actgcgggct cgtcgaattc cttcagggtt tccagtgctg catttgcatc gtttatatta
     2221 ttaaaggaaa gctccttgcc gtgaagctgc actgctgcgg caatactgcc gctgaccttt
     2281 ttcatttcct tgtaatatgc tgccccttga tgaggatttt ctccatatct caggtcctga
     2341 accttttcaa aggtcatggt cactgtctcc ggatacttcg gcaatccggc cttagaccat
     2401 aaataattgc ctatcaaagc atcatagctg gcagtatgtg aaaaaacctt ggcagcaagc
     2461 tcaaaccttg ttgcttcagt tgtatctccg ctgttattga tttcggaaag caccttagaa
     2521 tagtccttgg gatccacaac aaccgttaca tacttgtagt tcttggctgc cgccctgagc
     2581 atcgaagggc cgccgatgtc tatgttttct atagcttccg gaagctctgt gccttctttt
     2641 tctatggttt ttctgaaggg ataaagattg acgaccacca tgtcaatagt cccgatcccc
     2701 aattcacgga gcttatccat atgctccgcc ttgtccctta ttgccagaag cccgccgtga
     2761 atattgggat gcagtgtctt tacccttccg tcaagacact cgggatagcc tgttatatct
     2821 gatatcccaa taacctttat tcctgcctcc tcaagagcct tggcagtacc ccctgttgag
     2881 attatctcca ccccttgctt tgccagttcc ctggccagat caactattcc ggttttgtcc
     2941 gaaacgctta taagcgccct cttaatcata ccggcatcac acctcttcct ttaatatttt
     3001 aaccttccgc ccctctatgc ggagccttcc ttcgcaaaac agccctactg cagccggcag
     3061 cagcctgtgt tccacctgaa gcactctcgc agccagagtt tcagaagtat cttcaaaata
     3121 aaccggcaca gcctcctgga atattatagg gccgctgtcg gtgccttcat ccacaaagtg
     3181 gacggtagcc cccgatactt ttaccccgta ttccaaagct gccttatgta ctctttcccc
     3241 gtaaaatccc ttgccgcaga aagacggtat caatgaagga tgtatgttta ttatccgccc
     3301 ttcataggct tttaccaact ccctgtccag tatggaaagg aaacccgcca gaaccaccag
     3361 ttcaatatcc agcttttgaa gttccgccat gagcctttcg gcaagcagtt tcctgttgat
     3421 ataaattgcc ttgatgccgt gcttccttgc cctttccagg gcatatgcat tttcgttatc
     3481 ggatatcact gctgctattt ccccgttgat atcacctttg tccacggcat caatcaaggt
     3541 ctgcaggttt gttccgcctc ccgatacgag aacagctatc cttaccttcc gcaaaattct
     3601 ataccccctt cacctccggc aacactgcct atgatacagg ctttttcccc cagctcctca
     3661 agagtgccga gaattccttc cgcgtcctgt tttccgactg ccagtaccaa tccgatgccc
     3721 atattgaagg tgttgaacat ttctttttcc tctatgcttc ccaacctctg catcagcctg
     3781 aacaccggag ggatatacca gcttccgagg tctatctgag ccttaagccc tgcgggcagt
     3841 gtcctgggga tattttctat gaagccgccg ccggtaatat gggctatacc gtttatcctg
     3901 tgttttacta tcagggactg tactgtcctc acgtatattt ttgtaggctt cagcaattct
     3961 tctccaaggg tgccctccag ttcgtcaaat ctggtattta tatcaaaatt gtttatcccg
     4021 aaaaagattt ttcttaccag ggaatacccg ttgctgtgta tgccgctgga agcaatgcct
     4081 atcagcacat ctccctcttt aacattggag ccgtttatca cattgccctt gtccactatt
     4141 cctacggcaa aaccggccag gtcatattcc ccatccttat agaagcccgg catttctgcg
     4201 gtttctcccc caatcaaagc gcagcctgct tcaacacaac cgtcgcacac gccctttact
     4261 atatcagccg ctatgttcgg cataagcttg cctgtcccga tgtaatcaag aaagaacagg
     4321 ggttttgctc cgtgacaggc tacgtcattg acacacattg ccacacaatc aatcccaatt
     4381 gtatcatgct ttcctgtcat aaaagcgatc tgaagcttgg ttcctactcc gtcagttccg
     4441 gaaaccagga cagggttctt atagctttcc ctgtctatct cgaaaaaccc gccgaagctg
     4501 cccaattctc caagcactcc gggtatcatg gtgcgttttg tatgctgctt catcagcttc
     4561 actgcttcat agcctgcctc cacatctact cccgcagcct tgtatgtcaa tttttcatcc
     4621 ataggatccc acacctctgt ttcaaaatta ttttttcctc tcgaaaacat ccttgctgca
     4681 attttccggt atatccatag gataccttcc tgagaagcat gcggtacaga agccctcccc
     4741 cgtgcttccc accgaccgca ggattccgtt atagctgaga tatccaaggc tgtccgctcc
     4801 tattttttcc cttattgctt ccaatacact ggtcgcgccc acaagctcat ccctgtttga
     4861 agtttctatt ccgaaatggc aggaatgggt aattaccggg gagcttaccc taacatgaac
     4921 ctcttttgcc cccgcagcct tcagggcctg cacaatcttc ctgctggtag tacctctcac
     4981 tatgctgtcg tcaaccatta caacacgctt tccttcgata ttcgccttta aaggattcag
     5041 tttcaaggct acccctattt ccctggtaac ctgggaaggc tgtatgaagg tccttcctat
     5101 atatctgttc tttatcaaac ctattgcata gggaattcct gactgaagcg aataacctat
     5161 tgccgcagga gtccccgaat cgggcacgct gataacaaca tccgcgtcgg caggctgttc
     5221 ctccgccagt atcctgcctg ccttttccct ggcctggtat acgctaatgc cgtccatgac
     5281 gctgtccggc cttgcaaaat aaatatattc aaaaatgcat ccggcctgct tccctgttga
     5341 cctgcactgt atgctccgca taccgttttc atcaatgatt attatctccc ctggccttac
     5401 atctcttatg aagcttgcac ctatggtgtc cagggcacag gtttcggaag ccagcacgta
     5461 accgtttcct atccttccta taaccagcgg cctcagcccg tgactgtccc tcacccctat
     5521 aagcttgtca tcggtcatta taaccaggga ataagagccc cggataatgt ccatggtatc
     5581 cagtatggca tcctcaatgc ctttgctgca gcttcttgct ataaggcctg caataacctc
     5641 gctgtctata gtggtggtaa aaatcatacc ctggtcctcc agctcttccc gcaccgaacc
     5701 tgcattcacc agattcccgt tatgggcaag ggctatgctc cccttcttgt acttgaccaa
     5761 aaggggctgt gcattgtggg caaaactgtc cccggttgtt gaatacctta catggccgat
     5821 gctgatatcc ccctttaaaa ggttcaatat cttgtcatca aaaacctcgg acaccaggcc
     5881 catttcctta taacaggtta tgaccccgtc cctgttgacg gcaatccctg cgctctcctg
     5941 ccccctgtgc tgcaaggcaa tgaggccgta ataggtaagg ctttctattt ccatattgcc
     6001 tttgtcgtag atacctatta ttccgcattc ttccttaagt ttgtccatgg cttatctcct
     6061 aacacaacct ttttactact tccatatatc cttcttctac cttgcccata tcccttctga
     6121 acctatcctt atccatcttt tcaagggtca ccgcatccca aagcctgcag gtatccgggg
     6181 aaatctcatc tgccaggatt attttcccgt cgggagtctt gccgaattca agcttgaaat
     6241 ccaccagcag aatgcctttg gtaagcataa attcactcag aatctcattg attttcagag
     6301 ccatttcggt aatctttgct acttcctgct ctgttgctat acctattgcc tttatatgat
     6361 aatcatttac catgggatct cccagttcgt cattcttgta ggaaaactcc agcactgtgg
     6421 aattaagctt aacaccttcc tcaaggccta atctctttga aagggagccg gcagcataat
     6481 tcctggccag cacttccaga ggaattattt ttaccttctt gacgactacc tctctgtcac
     6541 ttatcctttc aacctgatga gtgtctatgc ctttttcttc aagaagcctg aagagctttt
     6601 ccgatatccg gttgttcata acacccttat ttattatggt tcctttttta aggccgttga
     6661 atgccgtcgc atcatctttg tactcaacta tatacagtgc ttcatcatct gttttgtata
     6721 ctttttttgc ttttccttca tatatcattc ccagcctttt cattagaaaa cctcctcttt
     6781 tatcttctcg tccttcttaa ttacatcctg tttcatcttt tccttgtagg ccttaagctt
     6841 atcctttaaa ttgccgtact ttaccgatag gatctgaact gccagaagcc ctgcgttttc
     6901 tgcgccgtca acggcaacag tggccacagg tattcctgaa ggcatctgta ctattgacaa
     6961 aagagaatcc aagccgtcca aaaccgacga ttttataggt acccctataa ccggaagcgt
     7021 tgtgaatgcc gccaaaaccc cgggaagatg cgccgccttc cctgccgcgg caattataac
     7081 ctcaaaaccg ttttcttccg ctttttttgc atattccgca gccctgtccg gtgtcctgtg
     7141 cgcagatatt acattaacct catagtctac ttcaaaagcc tttaaaattt caattgcctt
     7201 tcttacaacg gacagatccg agtcactgcc cattatgacc gcagcctttg gtttatctga
     7261 agacacaagc atcactcctt tttattgcta tttattttat cagctgaaat actccactcc
     7321 cgccttgaat atcagctgtt ccttgtctct gggcacattt ttgccgacgt tggccccggt
     7381 tctttccgaa tgccccatct tgccgaaaat ccttccgtcg ggacttgtta ttccttctac
     7441 agcttctatg gagccgtttg gattaaaggc agaatcgtat gtcggattgc cgtttaaatc
     7501 cacatattgt gtggcaacct ggccgttttt tataaggctg tccataattt ccctgcttgc
     7561 tgcgaacctt ccttccccgt gggataccgc tatggtgtga atatccccga ctttggactt
     7621 gctgagccac ggagacagtg aggataccac ctttgtcctt accatacagg atacgtgccg
     7681 ccctatcttg ttgaaggtca gggtcggtga gctttcgtcc atgtccatga tttctccgta
     7741 gggcagaagc cccagcttaa taagcacctg gaagccgttg cagattccaa gcatcaagcc
     7801 gtccttctcc cgcaggaatt tcataaccgc ttccttaacc cgggggttcc tgaatactgt
     7861 cgcaatgaat ttgcctgaac cgtccggctc gtcgccgctg ctgaagcctc ccggaagtgc
     7921 tattatctga gtatttccaa ttagccttac catttcattt attgaggctt ctatatctga
     7981 agggccgata tttctgaata cgaaaattga aggctcgcct cctgcttttt caaaggctct
     8041 tgcagtatcg tactcgcagt ttgtaccggg gaataccggt atgaatacct tgggccgtgc
     8101 aattttcgcg gaggatttga caatatttgt actattatag tatatattct gcggtttgtc
     8161 atcattatcc cctgaatggg ttgggaatat cctctccaaa ggagctttcc atgccttaag
     8221 cgcttcttca agctcaattt ttataccttt tatgcttata tatccggtgc tttccgtgac
     8281 tccgataatt tcataatcca tgccgctgaa tacgccgttc aggtcgcagg actgatccat
     8341 ctccagaata attgaaccgt attcgggagt aaacagatcc ctttcctcat agcccttttc
     8401 aaaggcaaag cccagcttgt tcccgaagca cattttgctg actgctgccg caattcctcc
     8461 aatcctaact gttgctgatg aaagcaccaa acccttctgt atcaattcat gtaccttgga
     8521 aaatatctta tgaagctttt caaaatccgg caggtccttg tcgtctcttt cagcccttaa
     8581 aagtatcacc ttgcttccgg attttttaaa ctcctgtgat actatcctgc cggcttccgc
     8641 tgtgtcaacc gcaaaggcaa ccagggtagg aggcacatta aggtccttga aggtaccgga
     8701 catgctgtcc ttacccccta tggcaggaac ccccagcatc atctgggcgt agaaggcacc
     8761 aagaagcgca ccggcaggtt ttccccactt ttcgggaacc ttgcccagct tctcgaagta
     8821 ttcctgaagt gtaagcctta ttctgccgta atcccctcca agggctgcaa cctttgcaac
     8881 cgcctcaacc actgcatata ccgctccgtg gaaggggctc cactttgcaa gcttcggatt
     8941 atatccgtag gtcattaccg ttgccgtatt tgtctctccg gaaagcaccg gcagcttcat
     9001 tgccagtcct tccgaaggag tttcctggta tatgcctccg aagggcatca gtattgttcc
     9061 tgcgcctatg gtactgtcga acctttcgac aagccccttc tgactgcata cattgagatc
     9121 cttaaggttc tcaagccatt ttcccttcaa gtcctccttg ctcttttccc aagcctgcag
     9181 cttattgaag cagctctccg catccggaga ctttatcagc acccgtgtac tctgtcttac
     9241 accgttggtg ttcaaaaagt cacggcttat atcaactatt gtctgtcccc gccactgcat
     9301 ccgaagcctg ttgtcccctg ttaccttcgc tacaaccact gcttcaaggt tttcagcggc
     9361 agcataattt ataaagcttt ccgcatcctc cggagccaaa agaaccgcca ttctctcctg
     9421 agattccgat atggccagct cggttccgtc caagccttca tatttcttgg gaactgcgtc
     9481 aaggtctatt aaaagcccgt cggccagttc tccgattgcc accgatacac cgccggcacc
     9541 gaaatcattg cactttttta tcatcctgct gaccatgggg tctctgaaca gtctttgaat
     9601 ctttctctct gtgggagcat tgcctttttg cacttccgct ccgcagctct gtatcgactc
     9661 ctcggtatgc tcctttgatg agcctgtagc tccgccgcag ccgtctcttc cggtccttcc
     9721 tcccaggagt attattacat caccctctga gggtgctttc ctgataacat tgcttttagg
     9781 cgccgcacct ataaccgcgc cgatttccat tctttttgcc acaaagtcct cgtcgtaaac
     9841 ctccgcaacc tgtcctgtgg caaggccgat ctgattgccg tatgagctgt agcctgcggc
     9901 tgcccctgtg gtaattttcc tctggggaag ttttccgggc agcgtttctt ctatgcttgc
     9961 ccttgggtct gcgcttcccg taaccctcat ggcaccgtac acataggacc tgccggacag
    10021 gggatccctt attgctcccc cgagacaggt tgctgcacct ccaaagggtt ctatctctgt
    10081 cggatggttg tgtgtttcat tcttgaacat cacaagccac tcctcccgct tcccgtcaat
    10141 atccgcatta acgataatac tgcaggcatt tatctcatcg gaaacctcaa ggtcatcaag
    10201 aagtcctttt ttcctcattt ctttcatgcc cattactgcc atgtccataa gggtgatatc
    10261 cctttcggag tctccgtata caaaggctct tgcgtccata taccttaaca gggctttttc
    10321 aatagcttcg gtatatttgc ccttctcaat ttctattttt tctattaatg tgttgaaggt
    10381 ggtatgcctg cagtgatccg accaataggt gtcgattact cttaactcag ttaccgtagg
    10441 gttccttttt tccgacgtct tgaagtattc ccggcagaac cggaggtctt cggaactcat
    10501 tgcaaaaccc ataacttcca taagccttgc aagtccggcc tcatccatgg ttataaaatc
    10561 tttgacttct tttatgtccc ccggttccgg ataaacagcc tccaggcttt caggcttttc
    10621 caggcttgct tcccttaatt caagaggatt aatgcaataa tttttgattt tttcaaattc
    10681 atcatcggaa atttctcccg aaagtataat cagtttcgca gtcacaacct tgggcctttc
    10741 cttctgggtc aatatctgaa ggcattgggc ggctgaatcc gccctctggt cgaattgtcc
    10801 cggaaggtac tcaattgcaa acaatctatc atttttatcg attatgattt tttcatcata
    10861 ggaattatct acgggaggtt ctgaaaagat tgtattcttg gctgaattgt attcactgtc
    10921 ggtgattccg gaaatgtcgt aacgatttac tatcctaaga tttgtgaggc tctttatccc
    10981 aaggttttcc ctgatatcat tgaacaagtc ctttgcggaa acggcataat cgtccttttt
    11041 ctccacaaat atccttctta cattgccatt catcccactt cacctcattt atactaatat
    11101 ctctttaata cggtgctgtt attcaccggt ttctgcggac accatgtatg atgcggacac
    11161 catgtatggt gtccctactt tacaatttga ttttatcata gtaaaattat ttaaaataat
    11221 taatattttt aatattaatg ataagaaggg cttatatatg gatattaact ttgaacttta
    11281 taaggttttt tattatgtgt caaaatccct cagtttttcc gaagcctcgg aggagctttt
    11341 cataacccag tcggcagtaa gccagtccat aaaacttctg gaggaaaaac tcaagtgtaa
    11401 gctttttttc aggaatacca agagagtaaa acttaccagg gaaggggaaa tattatatag
    11461 tcatattgag caagccttta atttcataaa atccggtgaa agaatacttg aggaaagaaa
    11521 ttcccttacc cagggtgaaa tcaggattgg aaccagcgat accatatgca aatattatct
    11581 tatgcccttt atcaaaagct tcaaccaggc ttaccccaat ataaagattc acctgacaaa
    11641 caggacttcc cccaggtgca tagagctttt gagaaaaggc agtgtggatc tgagcgttat
    11701 aaacattccc gaaaaactta attacagcaa tattattgta aaaaatacca aaagagtcag
    11761 ggatgtattc atagcaaatg aaaattttaa agagctgaaa ggcaggaagg tgcagctgaa
    11821 ggaactggaa gagtaccccc ttcttgtatt ggagaggaac tccaccacca gagaattctt
    11881 cgacagcctg atggcaaagc acaaggtatc cataaagccg gaaattgagc ttgggagtat
    11941 agatatactg atggaaatgt caaagatagg actcggaata gccttcgtgc cggatgaatg
    12001 tgtcaggatt gggcttgcga aggatgatgt attcataatt gatataaaag aagaaatccc
    12061 cgaaagaaat ctgggattcg tcatacattc aaatattccg ctgcctgtag cagcatcaaa
    12121 atttatagag cttcttgagg gagagcacta gctccccaag ctcccctttc agcaggtaag
    12181 atacgttttt cagatctgtt atatccttgg ctcccaataa cagcataagc accctgattt
    12241 cgtacaaaag ggctgccatg tacttttctg cagcatcata gccctgctcc agcagtatcc
    12301 tcagtattgt tcctcccaat gccgccatgt ctccgtccat gcagagggct ttaaccactt
    12361 cttccgcctt tgatatgccc cccgagcaga ttatgaacat atcactgctg gcctttctgc
    12421 actgaaaaag gctcagagca gtgggtatgc cccaattctc caggaagggg cattccctat
    12481 cgctgttccg caagctttca atgcttacaa agtcagttcc gcctttgcct ccgatgtcta
    12541 tatattttac tcctgccgcc tgaagctttc cggcagtttc aaaggatatt ccgaagcctg
    12601 tttctttaac tattaccgga acctcagcca gtgcggcaat ctttttgata ttctccagca
    12661 cacccctgaa gttccgatct ccttccgcca tgcacatttc ctggactatg tttatgtgaa
    12721 gctgtaccgc atctgcatca atcatgtcta ttgccttgta aacatcctct atatcccggt
    12781 ttgcacccag attggcaata actattccat ccctgtgtat atcccttacc cgcttaaagg
    12841 tatcagcagc cttgggattg tcaaaagcta ccgtctgtga acccacggct ataggaatgt
    12901 tgaatttgac cgcaagcctt gccagcttcc tgttaatatc gccggaatac tctgtccctc
    12961 cggtcattgc attaatcatc agggggctgt caatatattt gttcaggaac acacactctg
    13021 tgcttatttc atcaaaatcg gcatcaggga gggaattatt ctgcagtacc acatcggaaa
    13081 agccgttgta tcctgaaggc ttactcaaaa gaaaatactt cagatgctca tccttgcgtt
    13141 tctcacgggc tgatttctga tcttccattt gaaccacctc catatcacta ctgccaaata
    13201 tttaatatta ttatacgtat tctattatat cagcatgtat gccgtcaata ctcaataccc
    13261 ctcaggctaa cggtttgatg cattttgata tattgcttca atttcacctt tagacaacat
    13321 cctttcggta aaatatgcaa taacagctat tcctatcaaa tccatgacca ttcttacaag
    13381 catgaacttc aggccaaggg aagaggcttc aaatagtatc aggggtatct ttgtggttga
    13441 ccaggctccc agcataataa acacatttga caatttgctt ccttttttca gcagcacacc
    13501 ggcaacagga aatgctgcat acagcgggcc ggcagcggca gagccaagaa agaatgccag
    13561 cagtacacca attattcctg aatcgtcacc catatacttg atcatggttt cacgcttcac
    13621 ccatacatcc agcagtccaa gcagaataaa aatcggaggc aatattgcca tcatctcaag
    13681 caaattcgac catgtcagct gcaatgactt gctgccaacc gcaggataaa aaatgagtac
    13741 agccatgtta agtaaagcaa gtaaaaaaaa tactctatat cttttaacca accccgcttc
    13801 tttagtcgtc acataaccac ccccataacc aaagctacaa tcaaagaaaa tacaaaggcg
    13861 gcagtattcc taaccgcggc tgcccttttc ccgaagtatt ccacctccac cggaatcgtc
    13921 acaataccaa ccatcattag cgcagaaata aaagctgcta tttgcatata gccggcgccg
    13981 ctttttaaca atgctgccgc caatggaaat gcgataaatc cgggaactaa agtaacagaa
    14041 cctataactg cagcaatcag cacaccgtac cagccggatt ctcttcccaa taattttgag
    14101 atctgttcgg gtgataaaac tgccaataat atacctatta taatcaaaat agacaaaaat
    14161 tgaggcagta tgttttcaaa agacttccat gccttcttaa gagcaagtat agttctttcc
    14221 ttgcttttga gaagtgagac cgaaagtgcc aatatcgcaa taacatatag agacgctgtc
    14281 ataggcaaat cctccttgtt attttccgca ttcatcatga tgctgatgtt catgacaaac
    14341 agaaccggta gatttcaatg tgcctttcag gtatgcttcc gcagcttcct tggcataacc
    14401 ccttgcacct aaaacaacct ctataccttt ttcgttaaat atgtcaatgg cgccgccgcc
    14461 catacctcct gatataatta catttactcc catattattc agaaagttgg ggagaaaacc
    14521 gggcctatgt cccggattca aaatagattc gctttttact atccggtcat tctcagcttc
    14581 aaagatgtta aagttcatgc aatgtccgaa atgttccgtt accatatcat tttcacttgc
    14641 taccgctatc ttaatcattt tttatcctcc atattttcta atattttccc gacaggctcc
    14701 atccaatcgc cttcaaaaag ttctatcatc cctttgtcac aggctgcaga tagtctcggg
    14761 tctatgggca gcttggcaag aaccttaaga ttatgttctt cggcaatttc atctatatga
    14821 ctgtctccaa atatcttgta ttctttatca ttatcaggac atttaaaata cgacatgttt
    14881 tccaccaggc cgattatggg aatattcata atctccgcca tcctgaccgc cttggataca
    14941 atcattgaaa caagctcctg gggagatgta actataatga ttccatccat tgctattgat
    15001 tgaaaaacag taagaggcac atcaccggtt cccggaggca tatcaatgaa catgaaatca
    15061 acatcactcc atatgacctc cgaccagaat tgttttaccg tattggccaa tatgggacct
    15121 ctccatacta ccgggtcctt gtcattatcc ataagcagat ttatggacat aatgtcaata
    15181 cctgtatgac tttttatcgg atataatccc ttttcgctgc ctctggcctt ttcccttata
    15241 ccaaaggctt tgggtattga agggcctgta atatcggcat ccaatacggc tgaatgatag
    15301 cccattctgt tcattgtgac agcgagcatt gatgtaacca atgatttgcc tactcctcct
    15361 tttccgctga caacaccgat tactttttta atgctgctta atgcatgtgg tttctctgaa
    15421 aaatcaaatt cttcttttct ttcagtgcag ctttctgaac agctgctgca gttctgattg
    15481 cattgttcgc tcattttttt gtctccttca tgtaaatatt atattgttga agtatcaaaa
    15541 agcagcttcg tggttttgtt aaaaacctct ctgactgcat tgcctgccgg acaatcaata
    15601 tcgataacag taaggccctc attaatcgct ttcactgcct ccggatcaaa aggtatcctc
    15661 cccacaaagg gcagattatt tgtccggcaa tattcttcta tcctcgccgt atttgcaatg
    15721 tttgcatcat atttattaac gcaaactgct gcttttgtct gaaatgtcgc cgcagttttt
    15781 tctatgcgct ccatgtcact gatgcctgag agggaaggct ctgctactat taataccatg
    15841 tcagcaccgc ttagtgatgc aataacagga catcctattc cgggagagcc gtcaataatg
    15901 gcaagctccg gatcttttgc atttaatctc atttgctttt tgacctcggt aaccagcaat
    15961 cctgaagtac cgcttcccat tttaagctgg gctgttgaaa aaactttatc ttcatctgaa
    16021 tacagcatca attcccctgc aacagcatca tttaaagaaa ctgctcccgc agggcatacg
    16081 gcttcacaga caccgcagcc ttcacaggca aaataatcca ccttatatgc tttatccgat
    16141 aatattgctt caaaccggca attttgcctg caccgatcac accgtatgca caattcctga
    16201 tttatttcgg cctttgggag accgtaaaaa tctcttttct ccggttctga attccgaccc
    16261 ataatcaagt ggagattagg tgcgtcaaca tcgcaatccg cataggcctt tgcatgggaa
    16321 agctttataa atgcaccggc tattgttgtt ttacctgttc cgccttttcc gctaaggatt
    16381 aacagctgct tcatgccgca cctccttagt cacagtttca agcagagatg aaaacacggt
    16441 tctgtacttt tcactttctc ttactgctat ctttgcattt gaattcagtg ttccaagctc
    16501 attgtcgaag ggaattctgc ctaaaatcct gatgcctttt tcttcacaaa acctctctgc
    16561 agggttttct ccctcctggc atttgttaag aaccacccca taaggcttgc gaaacagctt
    16621 taccagttca taaaccatat taagattatg
//
LOCUS       DAOOAB010000056        16430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_4604_length_16430_cov_16.811969, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000056 DAOOAB010000000
VERSION     DAOOAB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4604_length_16430_cov_16.811969"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="PKU88_10575"
     CDS             complement(<1..1577)
                     /locus_tag="PKU88_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPX69758.1"
                     /translation="MNDRVRMLRQRSLDAVPAISLERAQIVTGVYKKYYGKVSIPVLR
                     ALVFKELMENKTICINEGELIVGERGPKPKATPTYPELCCHTVGDLDVIDKRQKIFFR
                     VSEEAKDIQRNEIIPYWKGRPMRDLMFAQMTQEWHDCYESGIFTEFMEQRAPGHTVCD
                     DKIYKKGFIGFKEDIEKQLAALDFVNDMDACNKQEELRAMSICADAIISFGLRHAEKA
                     LELALRESNPERKEELKQIADVCYNVPANAPGSFHEALQMYWFVHLCVITELNTWDAY
                     CPGRLDQHLYPFYKKGIDDGTLTPEKAEELLQCFWIKFNNQPAPPKVGITLQESGTYT
                     DFANINSGGLKADGSDGVNDVTYMVLDVIDEMRLLQPSSNIQLSKKNPDRFLKRACRI
                     ISKGWGQPSVFNADSVVEELVRQGKRIEDARNGGTSGCVETGAFGKEAYILTGYFNLV
                     KVLEITLNNGIDPRTGKKIGLETGDPREFKNYGELMDAYKKQLHHFIEIKIRGNNVIE
                     KLYADLMPAPFLSILTD"
     gene            complement(1582..2463)
                     /locus_tag="PKU88_10580"
     CDS             complement(1582..2463)
                     /locus_tag="PKU88_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPX69759.1"
                     /translation="MISGIVFNIQRYSIHDGPGIRTTVFLKGCPLSCIWCHNPESQSF
                     GPELMVYNKRCIGCGKCAEVCAHNAISDRNKCISCGSCTEVCHAKARELAGKKMSVRE
                     VIAQIEKDSIFYDESGGGVTFSGGEPLSQPEFLLELLKQCKKREYHTAVDTSGYGAAE
                     TIEAISGYTDLFLYDLKLPDDDKHIKYTGVSNKLILENLRLISSLGKKIFIRIPLIPG
                     INDDEEDIRATAEVIRGIAGVEQVNVLPYHNIAADKYARLGKHCSLKDIKVPSDGYME
                     HIAKKFFDYGVKVKIGG"
     gene            complement(2628..4052)
                     /locus_tag="PKU88_10585"
     CDS             complement(2628..4052)
                     /locus_tag="PKU88_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX69760.1"
                     /translation="MNYKRLLELILLNLDEGIIVTDAKANITFYKEPVTNIGGIDSHT
                     AVGKNILDVFPDLTPETSTFYNVLRTQKPIIEHIQHYTNYQGKKVSTVTSTIPIIENG
                     ELTGCFEIFKDLTQVMELSEKIMHLQEKLYKSSKGENQFNENGAKYTFENIIGISSSI
                     RQLKSKAGRIAGSNSPVLVYGETGTGKELLVQAIHNSDPKRKNKPFIAQNCAALPFTL
                     LESILFGTAAGSFTGAKDNPGLFELAHGGTLYLDEVNSLNTELQAKLLRVIQDGVVRR
                     VGAAKTRDVNVRIIASTNEHPEELVKRKLLREDLYYRLNVIYLELPPLRERKEDIDAL
                     VKHFIERNNESLGKKVTGVSEEVMKVFEEYPWPGNVRELEHLVESTMNLTDKKVISLE
                     DVQHYFDNRLKIAAINSEKPEKDIPVKNLNTAVAKYEKELIIKALKASNGNISMAARN
                     LGLPKQTMHNKVKKHNIKLTAEIR"
     gene            complement(4133..5497)
                     /locus_tag="PKU88_10590"
     CDS             complement(4133..5497)
                     /locus_tag="PKU88_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPX69761.1"
                     /translation="MIKNFILDTNVLVHDPYCISNFQDNNVIIPIMVVEELDSLKKRE
                     GMLGFHARSAAREINKLREYGSLSEGVCLPGGGTFRIELDHLESIELPDGLDLSKTDN
                     KILALTKNLQRENTGQPTILVTKDVYMAIKGDSLGITVQDYETDKVSVESIYTGYTEL
                     MLPSKDIEKIFKDGLKLPKQIDETIYPNEFIHVKSNDTSSNGVLTIFNGDRIVPLKYA
                     NNNAWGLVPINREQKMAFELLMDNNIHFVTLAGGAGSGKTILSTAVALQKVIEEEVYR
                     KIVFIKPVVPAGNDIGYLPGTEAEKLKPWMGSFYDAVDNLFGNRYRRKEKPSKSDAMA
                     KPTFTVDSFIEDYRQHGIIETKTFTFMRGRTLSDALVVVDEAQEITPHLAKMMLTRAG
                     FGAKFVFLGDPTDSQIDNVLVDARSNGLVYTIEKMKPFQITGHVTLNKVERSPLAQIA
                     EKYM"
     gene            5875..6693
                     /gene="zupT"
                     /locus_tag="PKU88_10595"
     CDS             5875..6693
                     /gene="zupT"
                     /locus_tag="PKU88_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPX69762.1"
                     /translation="MNSNAVLLAFGLTLIAGLSTGIGSLLAFFAKRTNTRLLSGVLGF
                     SAGVMIYVSLVDILPHANSILRASMGDRTGSWVSFISFFGGILLIAIIDKLIPSSENP
                     HESRRVEQMGQNYCDIKGNNMHLMKMGVLTAVAIGVHNFPEGIATFIAALKDPSLGIA
                     ITIAIAIHNIPEGIAVSVPIYCATGDRKKALVYSFMSGLSEPLGALLAYIFLMPILSD
                     QVFGIMFALVAGIMVFISLDELLPTAREYDEPHIATYGLVCGMALMGISILLFM"
     gene            6853..6929
                     /locus_tag="PKU88_10600"
     tRNA            6853..6929
                     /locus_tag="PKU88_10600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6887..6889,aa:Arg,seq:cct)
     gene            complement(6991..7938)
                     /locus_tag="PKU88_10605"
     CDS             complement(6991..7938)
                     /locus_tag="PKU88_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPX69763.1"
                     /translation="MNNNTSVIGSVLRIEKASIHDGKGLRTVLFLKGCPLRCRWCSTP
                     ESQSFGLQKGYAQDRCIGCGICVRSCPERALSISYDTQKVLTDLSKCKKCFICAAKCP
                     RRAIKQYGSLMSVKEVIGEISKDEIFFFHSGGGVTISGGEPLNQPEFVSEVLRECRAL
                     GIHTAIETSFHVSYANVEKTLPWLNVLYVDLKHMDKDLHKDWVGHENSLILDNIKKAD
                     QSEYPLEIIVRIPMIPGVNDTDSNLSAVAEFCKSIKKLKEIELLPYHRLGTDTYKHLG
                     MEYLLKDLAPPSQEHISERARFLTELNPGVPVRIGSGLG"
     gene            complement(7944..10340)
                     /locus_tag="PKU88_10610"
     CDS             complement(7944..10340)
                     /locus_tag="PKU88_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HPX69764.1"
                     /translation="MFQTTHEPKYMERIIRLKNRVLETRPEMDLENARILTEGFREAE
                     GEPLVVRKAKAFRKQCLEKTVTIWDDELIVGCSGSKTRAGILCADSCWSVLNDELETI
                     SERRYDPFYLKPEDRKIFEEEIRPYWKGRSNYEEWLAQIPDDTRALRDNGVVYIDRKA
                     VRGWGETTAGYEWMIREGVSGIIKVVEKRLSELDITIPGDYEKQYYLKALLIAADGII
                     KLAERYADEADRLAAAKKDPQRKKELLEIAQVCRHVPANPARSFREAIQSFYFYHICI
                     FMEQNAASYNPGRMDQYLWPYYKADIEAGRISPEEAQELLDCLWVKFSEPCLFQDAVT
                     AEFAAGYPMFQNVCVGGVDDTGRDAVNDLSYMILQATMDVQLYQPSLSVRYNLAKNPN
                     AFLRKVVELISLGTGFPAFHNDDVGIMMLMNKGIPLREAFNWNPCGCVETNLEGRLRQ
                     YTALADINLGSMVEFALLDGKNRKSGRYISERTGNPTNFLTYEEFLSAVKKQISYAIR
                     AVVKGSHVIDEIGKNRPCPALSLTFKECIEKAKDYAWGGAKYNTGNGIIMIGVADLIN
                     SIAAVRHIVYGTKQATMKKLLEALDNDFEGYEELHKACLDAPKYGNDDSMVDDIAGDM
                     FTFIADEIEKYSSKMGRMTPGILPVSGNTPFGLEVGALPSGRKAWKPLADGVSPSGGT
                     DFNGPGSVLKSVANIPHGRFVQGTLLNMKVEPAMLATENGIAQMMALLKSMCSLGVYH
                     VQFNVIDQEKLIHAQKNPEEYKGLLVRVAGYTAYFVELGKDVQDEIIGRTVQHRISAG
                     "
     gene            11029..12186
                     /locus_tag="PKU88_10615"
     CDS             11029..12186
                     /locus_tag="PKU88_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPX69765.1"
                     /translation="MVKRLMIFTLVVVLMASVVGCAKPAAPPADTGDSSAPKFDGELL
                     LGVMVPVTGSEATYGKDMENAIKIAVEEINAAGGLLGKEVKLTLGDDGCDPQMGSAAA
                     SKLVSAEVKAVLGGYCSGATIPTLKIYGDAKVPFVIIAANSTKIIEENPGWAFMINSP
                     GYHQANAAADCHEKLGTTKLALVHQGDGFSEDLATLTKKEWESRGKQVVGFDVVNKGE
                     QDFSSLVTKIKSSGAEGVYWTAYHADGALLIKQLRQGGFTGNIVVADGSSSVQLLEIA
                     GQAGEGVYCTSPPVVDFLPAAKEFISKYTAAYNQQPGPYSGLAYDGTHLLADAIKRAG
                     STDPDAIRSALEDTKDFKTLSGTITFTPEKVLEKSNFISIKGVGGKWVLAE"
     gene            12259..13176
                     /locus_tag="PKU88_10620"
     CDS             12259..13176
                     /locus_tag="PKU88_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPX69766.1"
                     /translation="MSFREIIIQQLFNGLLLGSFYGLVALGYSMVYGIIKLLNFAHAD
                     IYMVGGFCSLSLLAFFGGTFFGNGWLGIIAAMLITMIIVGLLGVLIERIAYRPLRDAP
                     RLSLLITALGVSMILYNSVMVITGGEYKAYKTGLGFEGFSLGSVHVTYTQAILIVAAV
                     SLMFVLHLFITRTMYGKAMRAISMDLDCCRLMGVNVNKIIALTFFVGSALAASAGMMA
                     GVYYGSLHFFMGFVIGIKAFTSAVIGGIGVIPGAMLGGVLLGLLESFGTQIPFIGSEW
                     KDVFSFSILILILIFKPTGLLGKKESERM"
     gene            13182..14186
                     /locus_tag="PKU88_10625"
     CDS             13182..14186
                     /locus_tag="PKU88_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPX69767.1"
                     /translation="MKEKQTAAARTAIKRDNPLAGLINRLYIIFDNKKIRYSVLAIAV
                     AVLLYLPMITSLYFMEVMTNVWFNVTVCLGLNIIVGFAGLLSLGYAAFFAVGAYTTGI
                     LISHYGVSFWLTLPVSVLMAIIAGLAIGAPTLKLRSDYLAIVTLGFGEIIRLAARNLQ
                     ITGRATGLVGIDRPVLFGMTLYKINHFYYAFLLVAILAIFVSYRLQDSRLGRAWKCIR
                     EDEDAAEAMGINVVAVRLYAFVIGSVFGAVAGSFMAVKMTAISPESFLFTQSVMILLA
                     VVLGGMGKIPGVILGAFAIVVFPEVFRGIGEYRMLIFGLLLLVLMIYRPQGLWPERHT
                     "
     gene            14229..15002
                     /locus_tag="PKU88_10630"
     CDS             14229..15002
                     /locus_tag="PKU88_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69768.1"
                     /translation="MLILEVKDLTLRFGGLTAVNKVSFGIDEGSIESLIGPNGAGKTS
                     LFNCLTGFYKPQEGDVLFQGKSIMKKKPHKITALGMARTFQNLRLFKNLTVMENVMSG
                     MHCRGSAGVAGAILKPPSQRAEEKRIREVALECMDFIGILDKKDRLAKNLPYGDQRRV
                     EWARALATQPKLLLLDEPAAGLNYDEKMQLIELIRRIRDERKMTVFLIEHDMGLIMKV
                     SEKIFVIDYGVKIAEGNVQEVQNNPKVIEAYLGKEEDAE"
     gene            14999..15715
                     /locus_tag="PKU88_10635"
     CDS             14999..15715
                     /locus_tag="PKU88_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX69769.1"
                     /translation="MSKVVLSLKNLATRYGQIYALKGISLDVCEGEIVTLLGSNGAGK
                     STTLKTISSLVPACSGKVEFYGKDITNAPPHDIVEMGIIHVPEGRRIFKELNVQENLE
                     MGSFTLKDDAERKRRMDRALELFPVLKERRKQMGGLLSGGEQQMLAIARALMTNPKVL
                     LLDEPSMGLAPIIVQGIMDIIKQINEQGTTILLVEQNAKAALKLAHRGYVLETGEIVM
                     EGNADVLSKDDSIVKAYLGH"
     gene            15909..16337
                     /gene="tadA"
                     /locus_tag="PKU88_10640"
     CDS             15909..16337
                     /gene="tadA"
                     /locus_tag="PKU88_10640"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPX69770.1"
                     /translation="MRKALEEAEKAFDKDEVPVGAVIVRKGEIIAAAHNCREILKDAT
                     AHAEILAIKEACEALGGWRLTDCDMYVTLEPCVMCSGAILQARIERLYIGAMDPKGGA
                     AGSTLNLFEDYHFNHRTEVIKSILEEECSQILKDFFKRKR"
     gene            16341..16430
                     /locus_tag="PKU88_10645"
     tRNA            16341..16430
                     /locus_tag="PKU88_10645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16375..16377,aa:Ser,seq:cga)
BASE COUNT         4348 a         3516 c         3619 g         4947 t
ORIGIN      
        1 tcatcggtaa gaatggaaag gaagggagcg ggcataaggt cggcataaag cttttctatt
       61 acattattcc ctctgatttt tatctcaata aaatgatgca gctgtttctt gtaagcatcc
      121 ataagctcgc catagttttt aaattctctg gggtccccgg tctccaatcc gatttttttc
      181 cctgttctgg ggtcgatacc gttatttaaa gtgatttcaa gcacctttac caggttgaag
      241 taacctgtca gtatataagc ttccttgccg aaggcacctg tttcaacaca tccgctggtt
      301 ccgccgtttc tggcatcctc aatgcgcttg ccctgacgaa caagctcctc cactaccgag
      361 tcggcattga atacagaagg ctgtccccag cctttgctta ttatcctgca tgctcttttc
      421 aggaaacggt cggggttttt tttgctcagc tgtatgtttg aactgggctg caacagcctc
      481 atttcgtcaa ttacgtccag caccatgtat gttacgtcgt ttactccgtc agaaccgtcg
      541 gccttaaggc ccccgctgtt tatgttggca aagtcggtat aggttccgct ttcctgaagg
      601 gttatgccta ccttcggggg ggcaggctgg ttgttgaatt taatccaaaa acactgcagc
      661 aattcctccg ctttttcagg tgtaagagta ccatcgtcaa tacccttttt gtagaagggg
      721 taaaggtgct ggtccagcct tcccgggcag tatgcatccc aggtgtttag ctcggtgatt
      781 acacacaaat gtacgaacca gtacatctgc agagcttcat ggaagcttcc gggagcattt
      841 gcgggtacat tataacagac atccgcaatc tgcttaagct cttcctttct ttccggatta
      901 ctctccctca gggccagttc cagggctttc tcggcatggc gcagtccaaa gcttattatt
      961 gcatcggcgc agatgctcat tgccctaagc tcctcctgct tgttacaggc atccatatca
     1021 ttaacaaagt caagggctgc aagctgtttt tctatatcct ccttaaagcc tataaagcct
     1081 tttttatata ttttatcatc gcacactgta tggccgggag ccctctgttc cataaactct
     1141 gtgaagatcc cgctttcata gcagtcgtgc cactcctgcg tcatttgtgc aaacatcagg
     1201 tcacgcatgg gcctgccctt ccaatagggt attatttcat tccgctgtat atcctttgct
     1261 tcctcgctga ccctgaaaaa tatcttctgc cttttgtcta ttacatccag gtccccgaca
     1321 gtatgacagc aaagctccgg atatgtgggg gtggctttcg gtttaggccc tctttcgcct
     1381 actataagct ctccttcatt tatgcagata gttttgtttt ccatcaattc cttaaagacc
     1441 agtgccctta agacaggaat ggataccttt ccataatact ttttgtagac accggttaca
     1501 atttgcgctc tttccaggga tatggcaggg acagcatcaa ggcttctctg cctcagcatc
     1561 ctgactctgt cgttcatagc ttcaacctcc tatctttact tttacaccat aatcaaaaaa
     1621 ctttttcgct atatgttcca tatatccatc ggaaggcacc ttaatgtcct taagactgca
     1681 gtgctttccc aatctggcat atttgtccgc tgcaatatta tgatagggca gaacattaac
     1741 ctgctctacg cctgcaatac cccgtatcac ctctgctgtt gcccttatat cctcttcatc
     1801 atcattgatt ccgggtataa gcggtatcct gataaaaatt ttcttcccca ggcttgaaat
     1861 cagcctaagg ttttcaagta tcagcttgtt ggatacgccg gtatatttga tgtgcttgtc
     1921 atcatccggg agcttaagat cataaaggaa aaggtctgta tagccgctta ttgcctctat
     1981 tgtctctgcg gctccgtatc cgcttgtgtc cactgccgta tggtactccc tttttttgca
     2041 ctgtttcaga agctccagca aaaattccgg ctgtgacagg ggctcaccgc cggaaaaagt
     2101 gacgccgccg cctgattcat cgtaaaaaat actatccttc tcgatttgtg caataacctc
     2161 ccggacggac atctttttgc ccgccaattc tcttgccttt gcatggcaca cttctgtgca
     2221 gcttccgcag gaaatgcact tattcctgtc tgatatggcg ttgtgagcgc aaacctccgc
     2281 acattttccg cagcctatgc atcttttgtt atagaccata agctcaggcc cgaaggattg
     2341 gctttcgggg ttgtggcacc aaatgcagga aagggggcag ccctttaaaa aaacggttgt
     2401 ccggattccc ggcccgtcat gtattgaata ccgttgaata ttgaatacta tccctgatat
     2461 gataatatca cccccgattg tatactatac aagctatgca aattacatgc catatcatat
     2521 atatccgaag agcttgcaac tattggcttc tcgaaaatac aaaaaaacaa cgtaccaaaa
     2581 ttggtacgaa taagtatcat attcggcacg tagtaccata aatggtacta ccttatttct
     2641 gccgtcagct ttatattatg cttttttacc ttgttatgca ttgtctgctt tggcaggcca
     2701 agattccttg cagccattga gatattcccg ttggaagctt taagcgcctt aattataagt
     2761 tccttttcat atttggcaac cgcggtattt aaatttttta caggtatatc tttttcgggc
     2821 ttttctgagt ttatggctgc tatcttaagc ctgttgtcga aataatgctg cacatcttca
     2881 agggaaatga cctttttatc ggtaagattc atggtgcttt ccacaaggtg ttccagctct
     2941 ctgacatttc ccggccatgg gtattcttca aaaaccttca tgacttcttc ggaaacgcct
     3001 gtaacttttt tccccagact ttcattgttg cgctcgataa aatgctttac caatgcatca
     3061 atgtcttcct ttctttccct gagagggggc aattcaagat atatgacatt gagcctgtaa
     3121 tagaggtcct ctcgcaagag cttccttttt accagctcct ccgggtgctc gtttgtcgat
     3181 gcaattatcc tgacatttac atccctggtt ttcgctgccc caactcttct tacaactcca
     3241 tcctgtatga ctctgagaag cttggcctgc agctctgtat taagggaatt tacctcatcc
     3301 aggtaaagtg tgccgccgtg agcaagctca aaaagtcctg gattgtcctt tgccccggtg
     3361 aagctgccgg cagccgtacc gaaaagtatt gattcaagga gagtgaaagg aagcgctgca
     3421 cagttttgcg cgataaaggg cttatttttt cttttggggt ccgaattatg gattgcctga
     3481 accagaagtt ccttgcctgt acctgtttct ccgtacacca gcaccgggga attgcttccg
     3541 gctatccgcc cggcttttga ttttagctgc cgaatggagg aacttattcc aattatattt
     3601 tcaaaggtgt attttgctcc gttttcattg aattggtttt cccctttgct gcttttatat
     3661 aacttctcct ggagatgcat tattttttct gacagctcca taacctgggt cagatctttg
     3721 aatatctcga agcagcccgt taattccccg ttttctatta tgggaatggt ggaggtaacg
     3781 gtagaaacct ttttcccctg gtagtttgta taatgctgta tgtgttctat tattggcttt
     3841 tgagttctca gtacattgta gaaggtgctt gtctccgggg taagatcggg gaagacatca
     3901 agtatattct ttccgacagc cgtatgggag tcaatacctc ctatgttggt aacaggctcc
     3961 ttatagaaag taatgttggc ctttgcgtca gtgacgatta ttccttcgtc aagattaaga
     4021 agtataagct ccagcagtct tttgtagttc atggcaatcc ctcacaaaag tattattagt
     4081 tttataatag aggactcttg agtgctaatc aagagtccaa agttcattgc acctacatgt
     4141 atttttctgc tatctgagcc agagggcttc gttccacctt gttaagggtt acatgtccgg
     4201 tgatctggaa aggcttcatt ttttctatag tgtacacaag cccgtttgaa cgtgcatcca
     4261 ccaggacatt gtctatctga ctgtccgtag gatcacccag gaaaacgaat ttggctccga
     4321 agcctgcacg ggtcagcatc attttggcaa gatggggagt gatttcctgt gcttcatcta
     4381 caactacaag ggcatcggac aacgtccttc ccctcataaa ggtaaaagtc ttggtctcaa
     4441 taattccgtg ctgcctgtag tcctcgatga aggaatccac ggtaaaggtg ggcttggcca
     4501 tggcgtctga cttgcttggc ttttccttgc gcctgtacct gtttccgaaa agattgtcta
     4561 cggcatcgta gaaggagccc atccagggct tcagcttttc cgcttcggtg cccggcaggt
     4621 agcctatgtc attgccggcc ggcacaacag gctttataaa tacgattttt cggtaaacct
     4681 cttcttcaat aaccttctga agtgctactg cagtggataa tattgttttt ccggaacctg
     4741 cgcctccggc caatgtaaca aaatgaatat tattgtccat aagcagttca aaggccattt
     4801 tctgttccct gttgatagga acaaggcccc aggcgttatt gtttgcatac ttcagcggga
     4861 ctatcctgtc tccgttgaaa attgtaagaa ctccattgct ggaagtgtca ttgcttttta
     4921 catgaatgaa ctcgttggga taaatggttt cgtcaatctg tttaggcagc ttcaacccat
     4981 ccttgaaaat cttctcaata tcttttgagg gaagcatcag ctcggtatag ccggtatata
     5041 tggattcaac agaaaccttg tcagtttcat agtcctgtac agtgattcca aggctgtcac
     5101 cctttattgc catataaacg tcttttgtca caagtatggt cggttgcccg gtattttccc
     5161 tttgaagatt cttagtcagt gcaagtatct tattatctgt ctttgaaagg tccaaaccat
     5221 ccggcagctc aatggactcc aaatggtcca attcaattct gaaggtgcct cctccgggta
     5281 aacaaactcc ttcgcttaaa ctgccatatt cacgaagctt gtttatttct cttgcagcac
     5341 ttcttgcgtg aaaacccaac ataccctccc tttttttaag actatccagt tcttcaacta
     5401 ccatgatggg gattatcaca ttgttatcct ggaaattgga gatacaataa ggatcatgga
     5461 ccagcacatt tgtgtcaaga ataaagttct ttatcatgca cttacctcca tatgagatga
     5521 tatatgcttc ctggtatatg gctccgcttt aggagtgatt atgtataata taaattctgt
     5581 caaatatcat taaattcctg caatcgtcga gaaaatttac ataaaattca caagggctat
     5641 tataataaga tttctgattg ttatattatt attttatgct gaacaaatac attctgaaaa
     5701 cttatttatc atatatatcc attggcaagc agaattttat taacattcaa gaaatttttt
     5761 tcaagtacat ttagaaaaat tataggaatg tattgtaaat gggaaccatt atcaatataa
     5821 tagaagggga tacatactat ttattattga tttttcgcag gagaggaata atatatgaac
     5881 agtaatgctg tacttttagc ctttggactc accttaatcg caggattatc taccggaata
     5941 ggaagccttt tggcattttt tgcaaagcgg acaaatacaa ggctgctgtc cggagttctg
     6001 gggttttcgg caggtgtaat gatatatgta tctttggtag atatacttcc ccatgccaac
     6061 agtattctta gagcgtccat gggagacagg acaggaagtt gggtttcatt tatttccttt
     6121 tttggaggaa tattgctgat agctataatt gataagctta ttccctcctc tgagaatccg
     6181 cacgagagca gaagagtgga acaaatggga caaaactatt gcgatataaa gggcaataat
     6241 atgcatctca tgaaaatggg tgtgcttaca gccgtggcta tcggagtaca taactttccg
     6301 gaagggattg caacctttat tgcagccctt aaggatccta gcttgggtat tgcaattaca
     6361 attgcaatag caatacacaa tatacctgaa ggtattgccg tatccgtacc aatttactgt
     6421 gctaccggag acagaaaaaa agcacttgtt tattctttta tgtcaggttt atcagagcca
     6481 ttgggagcgt tgcttgcata tatatttttg atgccgatat taagcgatca agtttttggg
     6541 ataatgtttg cactggttgc aggcattatg gtttttatat ccctggatga acttcttcct
     6601 actgcaagag aatatgacga accgcatata gctacatatg gattggtatg cggaatggct
     6661 cttatgggaa taagcatatt gctttttatg taaaaaattt tttggaaacc ggcataaaaa
     6721 agcaccgggt gcataaaaga catcatatta agcaactata ttgctgtaga aggcttaaat
     6781 acagcatagt gccaaaattt acgattgaca aagcaacgat ttttatatac tattttaatg
     6841 tgagtcgact gtgcacctat agctcagcag gatagagcga cggtttccta aaccgcaggt
     6901 cgggggttcg aatcccttta ggtgcaccat atatgagaat aactgtctaa ttaatagaca
     6961 gttttttatt ttttgaagtt ttggatgcaa tcatcccaac ccgctgccga ttcttaccgg
     7021 aactcccgga ttcagctcag tcaggaacct tgcccgttca gatatgtgct cttgtgacgg
     7081 cggtgcaaga tctttcagta aatattccat tcccagatgc ttataagtat cagtaccaag
     7141 gcgatggtaa ggcaacaact ctatttcttt aagttttttt atggatttgc agaattccgc
     7201 aactgccgac aggttggagt cggtatcgtt tactcccggt atcatgggaa tgcgtactat
     7261 tatctccaat ggatattcgg attgatccgc ttttttgata ttatccagga ttaatgaatt
     7321 ctcatgccca acccaatctt tatgaagatc cttatccata tgtttaagat caacataaag
     7381 aacattgagc cacggcagag ttttttccac attcgcataa gagacatgga agctggtttc
     7441 aatggcagta tgaatgccca gtgccctgca ctcccggaga acttcagaga caaattcagg
     7501 ctggttcagg ggctcaccac cgcttattgt tactccgcct cccgaatgga aaaagaaaat
     7561 ctcatccttg gatatttctc ctataacttc ttttaccgac ataaggcttc catattgctt
     7621 gattgctctc cgggggcatt tcgcagcgca gataaagcat tttttgcatt ttgataaatc
     7681 agtcagcacc ttttgagtat catatgatat agaaagtgcc ctctcggggc acgaacgcac
     7741 acatattcca catcctatgc agcggtcctg ggcatagcct ttttggagtc cgaagctttg
     7801 ggattcggga gtcgaacacc atctgcatct aagggggcac ccctttagaa agagcacagt
     7861 tcgaagccct tttccatcat gaattgatgc cttttctatt ctaagaacag aaccaattac
     7921 cgatgtatta ttattcatta aaatcacccc gctgaaatgc ggtgttgtac cgtgcgccct
     7981 attatttcat cctggacatc tttcccaagc tccacaaaat aagcagtata acccgcaacc
     8041 cttacaagaa gccctttata ttcttcagga tttttctggg catgaattaa tttctcctga
     8101 tctatcacat tgaactgaac atgataaaca ccaaggctgc acatgctctt cagcaaagcc
     8161 atcatctgcg ctattccgtt ttcggtagcc agcatggccg gttctacttt catattcagc
     8221 aatgttcctt gaacaaaacg gccgtgagga atattggcta ccgacttcag aactgaaccg
     8281 ggaccgttga agtcggttcc tccgctggga ctaacaccat ctgccaatgg tttccatgct
     8341 ttacggccgg atggtaaagc accaacctca aggccaaagg gagtatttcc ggataccggt
     8401 aaaatgccgg gggtcattcg ccccatttta ctgctgtatt tttcaatttc gtctgcaata
     8461 aaagtaaaca tatctccggc aatatcatcc accatggaat catcgttgcc atactttggt
     8521 gcatcaaggc atgccttgtg aagctcttcg taaccctcaa aatcattgtc cagtgcctct
     8581 aacagtttct tcatggttgc ctgcttcgtc ccgtaaacaa tatgccgaac tgcagcaata
     8641 ctattgatta ggtctgctac gccaatcata ataatgccat ttccggtatt atatttcgct
     8701 ccgccccagg cgtagtcctt tgccttctca atgcactcct tgaaggtcaa tgaaagggca
     8761 gggcaagggc ggttctttcc aatctcgtca atgacatggc ttcccttcac aacagctctg
     8821 attgcataag aaatttgctt tttaactgct gagagaaact cttcatatgt taaaaaattt
     8881 gtgggattac ctgttctttc ggaaatatac ctgccgcttt tcctgttctt tccgtccaat
     8941 agtgcaaact caaccatgct cccaagatta atatcagcaa gtgccgtata ctggcgcaaa
     9001 cgtccctcca ggttggtttc cacgcagccg cagggattcc agttgaaggc ttctcttaat
     9061 ggaatgccct tgttcatgag catcataatt cccacatcat cattatggaa agccggaaag
     9121 cctgttccaa gactgattaa ctccactact tttcttaaaa aagcattggg gtttttagcc
     9181 aggttatagc gaactgacag agatggttga tagagctgta catccatggt agcctggagg
     9241 atcatatagg aaagatcgtt aacagcatct ctcccggtgt catctacccc tccgacacaa
     9301 acattctgaa acatgggata accggcagca aactctgcgg tcactgcatc ctgaaaaaga
     9361 cacggctcac tgaacttcac ccaaaggcag tccaacagtt cctgagcctc ctcaggtgag
     9421 atgcgccctg cttcaatatc agccttatag taaggccata gatattgatc catacgtccc
     9481 ggattatagc ttgcagcatt ctgctccatg aaaatacaga tatgatagaa ataaaatgac
     9541 tgtattgctt ccctgaatga cctggcaggg tttgcgggaa catgacggca aacttgtgct
     9601 atttccaaaa gttccttttt cctctgcgga tccttttttg cagcagccaa acggtcagct
     9661 tcatcggcat atctctctgc taatttgatt ataccgtcag ccgcaattaa aagagctttt
     9721 aaatagtatt gtttttcata atcgccgggg atagtgatat caagctctga cagccgtttt
     9781 tccaccactt tgatgattcc cgagactcct tcacggatca tccactcata tccggctgtg
     9841 gtttctcccc atccgcgaac agccttacgg tctatataca ccactccatt atcccgaaga
     9901 gcacgcgtat cgtcaggaat ctgtgcaagc cattcttcat aattggatct gcctttccaa
     9961 taagggcgta tctcttcttc aaatatcttt cggtcctcag gttttaaata aaaggggtca
    10021 taacggcgct cgctgatggt ctccagttca tcattcagta ctgaccaaca agagtcagcg
    10081 cataaaatac ccgccctggt cttgcttccc gagcagccca caatcagttc atcatcccat
    10141 atggtaaccg tcttttcaag gcactgttta cgaaaagcct tggctttcct gacaacaagg
    10201 ggttcccctt cggcctcgcg aaaaccctcg gtaagtatcc ttgcattctc aagatccatc
    10261 tccgggcgag tctccagcac tctgtttttc agtcgtatta ttcgctccat atattttggt
    10321 tcatgtgttg tttggaacat acagacacct ccgttaaaaa tcatatgaga gatatcatta
    10381 cttcacttgt tgacacacga tttatatgcc gctatctaaa tcaagcatat cattcggagc
    10441 attttttgtc actcagaatc ttttttgcat atttatacaa ttttttttat ccttgacagc
    10501 attctgcgat taatgcttca atttaacgta aattctgcaa attccgtatg ttcagaaaac
    10561 agtctttaca ataactagaa tttatgatat atttctttac aagagtgtaa acagttccta
    10621 caatatgcat gtatttatgc ggaaataaca gcagttccga tatcagtgag tgtaccgcgt
    10681 gaaatatatg attccttcgt gcttgtcggt attaactatt gaatgcagct ctctttttta
    10741 aacgaatacg ttaattcata ttagcagtct accatatgca tatgcactaa tttattacta
    10801 ttatacataa ttataccata ctaagtaata tcatacctta taccgtcaaa gctttttttg
    10861 tgcaagttat aaaatcagat taaactcaga tgattatata ttagtgatat ctcttcgtta
    10921 actgtaccgg ggcctggaag agcctgctga tagatttgtc caatttattg ggtttaaacc
    10981 ctttgggcaa gaacccatgt gataaattaa ttaggggagg gaaatttgat ggtaaaaaga
    11041 ctaatgattt ttacgcttgt agttgttctt atggcatcgg tggtcggttg tgcaaaaccg
    11101 gctgcgccgc cggcagatac aggagattca tctgcaccta agtttgatgg tgaactgttg
    11161 cttggtgtga tggtacctgt taccgggtca gaagctactt atggtaagga tatggagaac
    11221 gctattaaaa ttgcagtgga ggagattaat gctgcaggcg gcctgttagg caaggaggta
    11281 aaactgacat taggagatga cggctgtgat cctcagatgg gcagcgccgc tgccagcaaa
    11341 ctggtatcgg ctgaagtgaa ggctgtactt ggaggatact gttccggtgc aacaatacct
    11401 acattgaaga tttatggaga tgccaaggtg ccctttgtta ttatagcggc caactctaca
    11461 aagataattg aagagaatcc gggctgggca ttcatgatca acagccccgg gtatcatcag
    11521 gcaaatgctg ctgctgactg ccatgaaaaa ctcggaacta ccaaacttgc ccttgttcat
    11581 cagggagacg gtttttccga agatttggca accctgacaa aaaaggaatg ggagagcaga
    11641 ggcaaacagg ttgtgggctt tgacgtagta aataagggag aacaggattt ctcatccttg
    11701 gttaccaaga ttaagtcctc cggtgccgaa ggagtatact ggacagctta tcatgctgac
    11761 ggagcgctgt tgataaagca gctgcgccaa ggcggattta caggtaatat tgttgtagcc
    11821 gacggttcca gctctgtaca gttattggag attgccggac aggcgggaga aggtgtatat
    11881 tgcacatcgc ctccggttgt agacttctta ccggcagcaa aagaattcat ttcaaagtat
    11941 actgctgcat ataatcagca gccgggtcct tattcagggc ttgcctacga tggtacacac
    12001 cttctggcag atgctataaa aagagccgga agcaccgatc cggatgctat ccgaagcgca
    12061 ttggaagata ctaaagattt taaaaccctt tctgggacaa tcacctttac acctgagaaa
    12121 gtcttggaaa agagcaactt tataagtata aaaggtgtag gcggcaaatg ggttctggct
    12181 gagtaaacat ggtggcgcag gtcaggtatc atattactcc tggcctgcgc atactttcat
    12241 aaatgaaagg gtgggcttat gtcttttcga gaaataataa ttcagcagtt gtttaacggc
    12301 ttgcttcttg gctcctttta cggattggtc gcacttgggt attctatggt ttatggaata
    12361 attaaactgt tgaactttgc ccatgcagat atatacatgg tgggtggatt ttgcagttta
    12421 tccttattgg ctttctttgg aggaaccttt tttggcaacg gttggcttgg aataattgca
    12481 gcaatgttga ttactatgat aattgttggt ttattgggcg ttctgataga aagaattgct
    12541 tataggcctt tgcgtgatgc tccgcgactt tcccttctta ttaccgcatt gggcgtttca
    12601 atgattttgt ataattcggt aatggttata accgggggcg aatataaagc ctataagacc
    12661 ggattgggtt ttgaaggctt ttcccttgga agtgtgcatg ttacatacac tcaggctata
    12721 ttgatagtag cggccgtttc attgatgttt gtccttcatt tgtttattac cagaacgatg
    12781 tatggcaagg cgatgagggc aatatccatg gatcttgatt gctgccgtct tatgggcgtt
    12841 aacgtaaata aaatcattgc cctgacattt tttgtcggtt cagcactggc tgcttctgcg
    12901 ggaatgatgg caggagtgta ttatggaagc ctgcatttct ttatgggctt tgttataggc
    12961 ataaaagcct ttacatccgc tgttatcgga gggattgggg taattcccgg cgctatgctg
    13021 ggcggagtat tgctgggctt attggaatcc tttgggacac aaattccttt tatcggctca
    13081 gaatggaagg atgtattttc cttttcgata cttattctta tactgatttt taaaccaaca
    13141 ggacttttgg gtaaaaaaga aagcgagagg atgtagagtc tatgaaggaa aaacagactg
    13201 cagctgctcg gacagctata aaacgtgata accccttggc aggacttata aatagactgt
    13261 atataatttt tgataataaa aaaatacgtt acagcgtttt ggcgattgcc gtagctgtgc
    13321 tgctttattt gcctatgatt accagtttgt attttatgga ggttatgacc aacgtttggt
    13381 ttaacgtgac tgtatgtctt ggccttaata taatagttgg gtttgcgggg ctgctcagcc
    13441 ttggctatgc agcatttttt gcagtgggcg cttataccac gggaatactg atttcacatt
    13501 atggagtcag cttctggctt actcttccgg tcagtgttct tatggcaatt attgccgggc
    13561 tggcaattgg agcaccaacc ttgaagctga ggagcgatta tttggccata gttactctgg
    13621 gttttggtga aattatcagg ctggcagcca gaaatttgca gataaccgga cgtgccaccg
    13681 ggctggtcgg tatagatagg cctgtcctgt ttggtatgac cttgtataag ataaaccatt
    13741 tttattacgc atttttatta gtggctatcc tagccatatt tgtatcgtac agactgcagg
    13801 attcgagact gggaagagca tggaagtgta tcagggagga cgaggatgct gcagaagcaa
    13861 tgggtattaa tgttgttgcg gtcagacttt atgcttttgt aattggctct gtcttcggag
    13921 ccgtagccgg ctcttttatg gcagtaaaaa tgacggcaat atcacctgag tcctttttgt
    13981 ttacccagtc agttatgatt ttactggcgg tagtacttgg gggtatggga aaaattccgg
    14041 gcgtaatact gggggctttt gcaatagtag ttttccccga ggttttcagg gggattggcg
    14101 agtataggat gttaatattt ggtctgctat tattagtttt aatgatttat cgtcctcagg
    14161 gcctgtggcc tgaacgccat acttgatatt tactgcgggg aaaaaattta tatcggacgg
    14221 ggtgaacaat gttgatttta gaggtgaaag acttaacatt aagatttgga ggacttacag
    14281 cagtcaataa ggtgagcttt gggattgacg aaggctctat tgaaagcctg ataggaccta
    14341 acggtgccgg gaaaacatcg cttttcaact gcctgaccgg tttctacaag ccccaggagg
    14401 gggatgtcct gttccagggc aaaagcatta tgaagaagaa accgcataag atcacagctt
    14461 tggggatggc caggactttc caaaacctta ggctgtttaa gaacttaact gtcatggaaa
    14521 atgtcatgtc cggaatgcac tgccgcggca gcgcaggcgt agcgggagcc attttaaagc
    14581 ctccttcaca aagagcggaa gaaaaaagaa tcagggaagt ggctttagaa tgtatggatt
    14641 ttatcggcat tttggacaag aaagatagat tggcaaagaa tttaccctat ggggatcaaa
    14701 gaagggtaga gtgggcaagg gcactggcaa ctcagccaaa gctgctgctg ctggacgaac
    14761 ctgccgccgg actgaactat gatgaaaaaa tgcagttgat agagctaata cgccgtattc
    14821 gggatgaaag aaaaatgacc gtttttttaa tagaacatga tatgggcttg attatgaagg
    14881 tctcggaaaa aatatttgta attgactacg gagtcaaaat tgccgagggc aatgtgcagg
    14941 aagttcaaaa caaccccaag gtaatagagg catatttggg gaaggaggag gatgccgaat
    15001 gagtaaagta gtcctttccc tgaaaaacct ggcaacccga tacggacaga tatacgcatt
    15061 aaagggtatc agcctggatg tctgcgaagg ggagatagtt acgcttttag gctccaacgg
    15121 tgcaggaaag agtacaactc ttaaaactat atccagtttg gtaccggcat gttccggaaa
    15181 ggtggaattt tacggaaaag acattacaaa tgcgccgccc catgatattg ttgagatggg
    15241 aataattcat gtccccgaag ggcgaaggat ttttaaagag ctgaacgttc aggaaaactt
    15301 ggaaatgggt tcatttacac taaaggatga tgccgaaaga aagcgaagaa tggacagggc
    15361 actggaactg ttccctgtat taaaagaaag gcgtaagcaa atgggggggc tgctctcagg
    15421 gggtgagcag cagatgctgg ctatagcgcg ggctctcatg actaacccta aggtgctttt
    15481 gctggacgag ccctcaatgg ggttggcacc cataattgtt caagggataa tggacattat
    15541 caaacaaatt aatgaacaag gcactacaat cctgcttgtt gaacaaaacg caaaggctgc
    15601 ccttaagctt gcccacagag ggtatgtgtt ggaaacggga gaaatagtga tggaagggaa
    15661 tgctgacgta ttaagcaaag atgacagtat agtgaaggct tacctgggcc attaaaataa
    15721 agtattaaat cccaatacaa atgaagtatt gggatttttt aatttttatt attagttttt
    15781 tgctgcggtt gtaaatttat ggcagctcat tgacaattca acgtcttaaa tgataatata
    15841 tcaaggtaaa ccgcataata atatatatta attatgaatt ggcgtggtag aaattggatg
    15901 ctctttttat gagaaaagct ttggaagaag cagagaaggc ttttgacaag gatgaggttc
    15961 cggttggtgc tgtcatagta aggaaaggtg agataatagc agcagcgcat aactgcagag
    16021 agattttaaa agatgcaacg gctcatgcag aaatacttgc aataaaagag gcctgtgaag
    16081 cactcggagg ctggcggctt acagactgtg acatgtatgt gactttggag ccctgcgtta
    16141 tgtgcagcgg cgccatattg caggccagaa tagaaaggct ttatataggc gccatggatc
    16201 cgaaaggcgg tgcagcaggc tcaacgctga acctatttga agattatcat tttaaccaca
    16261 gaaccgaggt aataaagagc attctggaag aagaatgcag ccaaattctg aaggattttt
    16321 ttaaaagaaa gcgataacac ggagaggtgt ccgagtggtc gaaggtccat gactcgaaat
    16381 catgcagggt gtcaaagccc tcgagggttc gaatccctcc ctctccgcca
//
LOCUS       DAOOAB010000057        16042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_4753_length_16042_cov_14.707700, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000057 DAOOAB010000000
VERSION     DAOOAB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16042
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4753_length_16042_cov_14.707700"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..815
                     /gene="ftsE"
                     /locus_tag="PKU88_10650"
     CDS             129..815
                     /gene="ftsE"
                     /locus_tag="PKU88_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPX69771.1"
                     /translation="MIQLRSVSKEFADGNKVLRNINLTINNGEFVFLVGSSGAGKSTI
                     IKLLLKEMEPTTGTIIVGDKDITKYKRREIPFHRRNIGVVFQDFRLLQEKTVYENVAF
                     AMEVIEAPPKEIRRQVPIILSMVGLSKKAGLYPQQLSGGEQQRVSLARAMVNSPSILI
                     ADEPTGNLDPDMSWDIMKTLSEINQRGTTVLMATHASDIVDKMKKRVVEIENGIITRD
                     EMKGGYSSEA"
     gene            805..1701
                     /gene="ftsX"
                     /locus_tag="PKU88_10655"
     CDS             805..1701
                     /gene="ftsX"
                     /locus_tag="PKU88_10655"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPX69772.1"
                     /translation="MKLRTGKFFIREGFRSLKRNSTMSAAAITSVIAALLVIGIFFII
                     VINIDYAATKLESQIEMMVYLEDGLSENIIDTMETEIRSINGVKSVEFISKDTALSNL
                     EKNWGENSYLLEGLEGDNPLPDAFLITLSDPSDASGVALSVSSISNIEKVVYGKEELA
                     KLLNATYVMRMSSVVIILILLFISIFIIANTIKLTLYARRREIGIMKYVGATDWFVRM
                     PFIIEGIVVGMIGAVVSTVILGVGYYYCSGLVKNQMIGFMSISLIPFNQIIVSMVILL
                     IIVGVVIGAVGSLISVRKFIKV"
     gene            1713..2852
                     /locus_tag="PKU88_10660"
     CDS             1713..2852
                     /locus_tag="PKU88_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPX69773.1"
                     /translation="MNKRIAAALIALMLSIPCANVYAKNPTEIDELQRNQDKLNQQIK
                     QMQSSINKIKTEKVSVAKAIEDLDSKLNQAEDELTGVENQLSQLENQIAVTTRELERA
                     SSEASNQKELLKKRVRVMYENGNVGYMSVILNSASFSDFISRVDFLKKIISFDMNLLS
                     KMKSYRDSVADKRSQLASELEEKERLKNEIGKKKEEVETAKQVKEMTLKEILSDLKEL
                     ERQEDKLLEQSKEIAKKIVELQSKEKYIGGELTWPAPGYYKITSQYGNRTHPILKKKK
                     MHTGIDIAVPSGTSVIAANAGKVIYSGYNGGYGNTVIIDHGGKISTLYAHNSKLLVKV
                     GDKVEKGQTISKSGSTGLSTGPHLHFEVRENGQHVDPMKYLTGKK"
     gene            2985..3926
                     /locus_tag="PKU88_10665"
     CDS             2985..3926
                     /locus_tag="PKU88_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPX69774.1"
                     /translation="MKKAITAVIILLLSAMALSGCMESGKKPADQRLKIYVSFYPMYF
                     LASEIAGDKAEVITMVPAGAEPHDWEPAPKTAAEISKADMLIYNGAGMEPWLDKILPN
                     INEKRTRLVDASQGIELLKSSEHDEEEEDIEENHEHGIYDPHIWVSPIRFRQQARNVY
                     EALLEIDPENSEYYGKRKIELDSRLSDLGKDIWDASKHFKSNTIVVSHESFGYFANDF
                     LLEQIAIRGINPQEEPSPSKMVELIRKCREKKVKYVFFEKLTSPKLSETLAREVGAGV
                     LVLNDAAGLSEKDIKSGKDYITVMYENLENLKKALGD"
     gene            3927..4679
                     /locus_tag="PKU88_10670"
     CDS             3927..4679
                     /locus_tag="PKU88_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPX69775.1"
                     /translation="MGAEIIRVENITFGYGKAKVLDNVSTSVIDGDFIGIVGPNGSGK
                     STLLRIFLGLIKPQKGTVRVFDTDIEKFNQWGKIGYIPQKATSFNQGFPATVEEVVSA
                     NLYPVVGLAGRIKKTHCEKVDEALQIVGMLEQKKSMVGRLSGGQQQRVFIAKALVSAP
                     RVILMDEPTVGIDQNSEESFYELMGFLNREKGITLVMVTHDIGAIDNRVNRVLCLSGG
                     KLYEHDCAVSRASDIFKEAYGKRDSLPIIHRH"
     gene            4703..5512
                     /locus_tag="PKU88_10675"
     CDS             4703..5512
                     /locus_tag="PKU88_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPX69776.1"
                     /translation="MFELFNYEFMQRALAAGLLVAVICPLIGTFVVMRRLSMIGDTLS
                     HASLAGIAAGMLGGLYPLWSALIFSLAAAIGIEKLRKHFPQYAELSISIVLSASIGLA
                     VVLISLANSFNADLMSYLFGSIIAVNALDIYIILGLSIIILVSVGLFYKEFFYMAFDE
                     EGAELAGMPVSSLNMYFTVLTAMTIVVSMRVVGMMMVSSLMVVPVACSLLISKSFKNT
                     IFLSVFFALISVVIGLFSSYWFDLAPGGSIVLTSVFILIAVIIVKRIFRMQ"
     gene            5543..5980
                     /locus_tag="PKU88_10680"
     CDS             5543..5980
                     /locus_tag="PKU88_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPX69777.1"
                     /translation="MKDIEKLETELKEKGYKLTEQRRSIIEAFNENPGHHTAQKIFEL
                     VKSKLKGINFSTIYRNLELLSTLEIISKLYDESGISHYELFGRTHHHHIICKGCGETR
                     EMDICPYAGLDEGQLSAMGFKATSHKFEIYGYCSKCRLLKEQD"
     gene            6078..7280
                     /locus_tag="PKU88_10685"
     CDS             6078..7280
                     /locus_tag="PKU88_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPX69778.1"
                     /translation="MINKRAAIFWAIILVIVTAFGTFVVTNTIAISLGNKVVIAQKDF
                     EAIKSMDKLMGLKEYIKSNYVEGAEGNKLIEGAIKGMFESLGDPYSVYMTKEEFKNFN
                     ETTKGSYGGIGVIVSRSEDGYVTVVAPIEDTPGEKAGLKTNDKIIKVDDKDIVGIDLE
                     EAVALMKGKKGTKVTLTVMRDNVREPKIFEITREEIILKTVKSNMMENSIGYIRITMF
                     DEDTGSEFKKALKELTSQNMKGLILDLRQNPGGFINQCVDVADELLDEGLVVYTEDKA
                     KKREDYKSGKGKLEVPFVVLIDEGSASASEIVSGAVKDRKAGLLIGVKTFGKGLVQSI
                     EQLKDGSGIKLTTQKYYTPNGISINKIGIQPDIEVKAPEPEEGQNPEDVKDVQMERAV
                     AELLKQIK"
     gene            7296..8591
                     /locus_tag="PKU88_10690"
     CDS             7296..8591
                     /locus_tag="PKU88_10690"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPX69779.1"
                     /translation="MFTVLKIMGLIFGQFGSFLMNELFWLIALIVILIYRKNSDIEAK
                     MLGSSYKLHYKVAGSFIVGLTAGLIGSLIVTLVGISIEDYTRSGGGSLIEGITYIWAV
                     AILLSAINPRYLCFSYAGGIIALMNLVFGFPSVNVPGLMALIGVLHLVESLLIWMDGS
                     SYSVPLFLRGKDGKTVGGYMMSRIWPIPLVAFAVILGIETGGVSLGGIIDMPGWWPLL
                     KHSAAGSRHGLAYLPLVVPVVLGYGDLALTRTPEQKCRNSALRLAGYSLVLITLSVIA
                     SKLRLFAFAAAVFAPVAHEMLIQYGVKEEEEGKPYFANKETGLRVLYVKKGSVACKMG
                     IVPGDTILRINGTALLSEKQLAEFLASCPVFIWVEFEKYEGELITAEYSDYRNGIGDL
                     GILIVPHNAKLYYEINRSSSLAKRFIKRFISNRNRDIDM"
     gene            complement(8557..8730)
                     /locus_tag="PKU88_10695"
     CDS             complement(8557..8730)
                     /locus_tag="PKU88_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69780.1"
                     /translation="MKEENNEFIKVFNYFENANARNRDAFIELLNYTIKNKRGSKTMT
                     SSVTYQYPCSGLK"
     gene            8864..9973
                     /gene="ugpC"
                     /locus_tag="PKU88_10700"
     CDS             8864..9973
                     /gene="ugpC"
                     /locus_tag="PKU88_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPX69781.1"
                     /translation="MANLSLKHVKKVYPGNVTAVKDFNLDIEDKEFIVLVGPSGCGKT
                     TTLRMIAGLEEITEGELYIGDRLVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     LKKIPKAEIDERVRKAAKILEIEPLLDRRPKALSGGQRQRVALGRAMVREPKVFLMDE
                     PLSNLDAKLRVQMRTEIAKLHKRLNTTFIYVTHDQTEAMTMGTRIVIMKDGDIQQIDS
                     PQKAYDKPANLFVAGFIGSPQMNVFRGNVVEAGGKAALELPDGTALGLTDDAATRLKD
                     SGYVSKEVYVGIRPEHLHDAEVDPLTKDYSVITAKVEVAELMGSETYLHFTKCGVQAI
                     ARVDAKTSMRAGDTAEVHLHWDKIHLFDIETEKAI"
     gene            10058..11050
                     /locus_tag="PKU88_10705"
     CDS             10058..11050
                     /locus_tag="PKU88_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX69782.1"
                     /translation="MADLQRLLAQAARITEKRMAVITPNERLAWPEDSDIDITFEEAC
                     KNSGKNFRGWNIYMPEDAGTRFFLCISADSYYESEVGKLVMLLLNSAFEKDDSASAYL
                     KRALEGNLDAAGLSMLEENLKSCLPGYVLLIDNIENSRDEIIEILENTMDIKTELIYE
                     NRIIAVAEAENITESCSSFIKNVLSELLIECNAVIGGRAAKASELYPLYRNCMEALSL
                     KHIYGLGDNVLDYDGMYGYRVAYNLDPRLKEFLRRKVFTAEFSDMVKGELGITIEEFF
                     RNNLNLTDTAAKLYIHRNTLLYRLEKIYKCTGFDLKRFEDSWLFRLAWIVSRER"
     gene            11212..13194
                     /gene="uvrB"
                     /locus_tag="PKU88_10710"
     CDS             11212..13194
                     /gene="uvrB"
                     /locus_tag="PKU88_10710"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPX69783.1"
                     /translation="MDRFKLVSDYKPTGDQPEAIEKLAKGILAGEKYQTLLGVTGSGK
                     TFTVANVIEKVQKPTLVLAHNKTLAAQLCSEFKEFFPHNCVEYFVSYYDFYQPEAYVA
                     HSDTYIEKEADINDEIDKLRHSATAALLERKDVIIVASVSCIYGLGDPEDYRSLVLSL
                     RKGMVRDRDEVLRKLIDIQYDRNDINFVRGTFRVRGDIVDIFPAASSDKAIRVEYFGD
                     EIERILEIDSLTGNITGERQHVYIYPASHFATTQAKLEAAISNIEAELIERVKELKEQ
                     DKLLEAQRLLQRTNYDIEMMKEVGYCSGIENYSRHISGRAPGSSPFTLLDFFPKDYLL
                     IVDESHVTIPQVRGMYFGDRSRKESLVDYGFRLPSAFDNRPLNFEEFEQRLNQVIYVS
                     ATPAKFELERSTCVAEQIIRPTGLVDPEIEIRPVKGQIDHLIGEVRERAANNQRVLVT
                     TLTKKMAEDLTAYMKELDIRVRYLHSDILTLERMKIIRSLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSEISLIQTVGRAARNVDGKVIMYADNITDSMRRAISET
                     NRRRKIQMDYNEKHDIKPQTIRKAIHEIIEATTAAEESAKYGIKHDIEFMRKDDLLRL
                     IAALEEEMKDCARALQYERAAELRDKIIELRHRLKK"
     gene            13206..16031
                     /gene="uvrA"
                     /locus_tag="PKU88_10715"
     CDS             13206..16031
                     /gene="uvrA"
                     /locus_tag="PKU88_10715"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPX69784.1"
                     /translation="MAKDKIIIKGAREHNLKNIDVEIPRDKFVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDLDYIEGLSPAISIDQKTTNRNPRSTVGT
                     VTEIYDYLRLLYARIGTPHCHVCGKEISQQTVDQMVDYINTLEPGTRIQLLAPVIQGK
                     KGEHQKLLEEIKKSGYVRARIDGEIADLNEEIKLEKNKKHTIEVIVDRIIVKEGVNKR
                     LTDSLETVLKLSGGTVLIDIIGKEERLFSQNFACVECGISIEDPAPRMFSFNSPFGKC
                     PVCDGLGELRRIDPDLVIPDKTKSVLEGAIEPWKNLDETSWYYNVVNAAAKHYGFSMN
                     TPICELDEKAVQILLYGNGGEKIKVKYQREYGSGEVMMAFEGIVNNLERRYLETKSNY
                     MRYEIENFMSSVPCPECKGARLRKESLAVTLGGLSIAELCSLSVYKIMEFLGSLKLTE
                     KQRIISKLVLKELHERLEFLVTVGLEYLTLSRTAGTLSGGEAQRIRLATQIGSSLVGV
                     LYILDEPSIGLHQRDNGKLLKALRNLTDLGNTLLVVEHDEETMHAADYIIDMGPGAGA
                     HGGKIVAAGSFDTILACEESVTGQYLSGKKQIPIPQERRKPNGKWIEIKGARENNLKD
                     LDANIPLGVFTCITGVSGSGKSTLINEILYKELSHRLYGSKLRAGEHKWIKGIEHIDK
                     VIDIDQSPIGRTPRSNPATYTGAFDIIRGVFAATPEAKMKGYNNGRFSFNVRGGRCEA
                     CKGDGIIKIEMQFLPDVYVPCEVCKGKRYNRETLEVRYKGKNISEVLNMTVEDALEFF
                     KNIPRVQKKLQTLFDVGLGYIKLGQPSTELSGGEAQRIKLAYELSKRSTGKTVYILDE
                     PTTGLHIADIHKLLGILDRLVDSGNSIIVIEHNLDVIKRADYIIDLGPEGGDRGGTVI
                     AQGTPEEICKVETSYTGQFLKKILAKY"
BASE COUNT         4933 a         2907 c         4239 g         3963 t
ORIGIN      
        1 tattacaaaa caaaaacatt ggcattttta aaggattttt cttcatttct gtagaagtat
       61 aagttgtttg agtcgaatct gcactatatt tgttttgtat tgacggtttt ggtaaggagt
      121 gtgatagatt gatacagctg aggagtgtaa gcaaggagtt tgcagatggc aataaggtat
      181 tgaggaatat caacctgact ataaacaacg gagaattcgt ttttcttgta ggttccagcg
      241 gagccggcaa gtcaactatc ataaaactgc tgctcaagga gatggagcca accacaggca
      301 ctataattgt gggagataag gatataacaa aatacaaaag aagggagata ccttttcaca
      361 gaaggaatat aggcgtagta ttccaggatt tcagactgct gcaggagaag acggtatatg
      421 aaaatgttgc ttttgccatg gaagttattg aagctcctcc gaaggaaata aggcgtcagg
      481 ttccgatcat actgagcatg gtcggactga gcaagaaggc aggcctttat cctcagcagc
      541 tttcgggggg ggaacagcag agagtatcct tggccagggc aatggtcaac agtccttcaa
      601 tacttattgc cgatgagcct acagggaatc tggaccctga tatgtcatgg gacattatga
      661 agaccttgag cgaaataaat caaagaggta cgactgtatt aatggccacc cacgccagtg
      721 atatagtgga caaaatgaag aagcgggttg ttgaaataga aaatgggatt attaccaggg
      781 atgaaatgaa aggtgggtac agcagtgaag cttaggacag gtaagttttt catcagagaa
      841 ggatttagaa gccttaaaag aaacagcacc atgagtgctg cggcgataac ctcggtaatt
      901 gcggcactgt tggttatagg gatattcttt attatagtga taaatataga ttatgctgca
      961 accaagctgg aatcacaaat agagatgatg gtatacctgg aagacggttt gagcgaaaat
     1021 atcatcgata ctatggaaac cgagatcaga tcaatcaatg gtgttaaatc cgtggagttc
     1081 atatccaagg acacagccct cagcaatctg gagaaaaact ggggcgaaaa ctcctatctt
     1141 ttagaggggc ttgaaggaga taatccgctt ccggatgctt ttttaatcac cctttcagat
     1201 ccttccgatg catccggtgt ggcattgtcg gtatcatcca tatcaaatat tgaaaaggtg
     1261 gtatacggca aagaggagct tgcaaaactc ctgaatgcga cttatgtgat gaggatgagc
     1321 agcgttgtta ttattctgat actgctgttc atatcaatat ttattattgc aaataccatt
     1381 aagcttaccc tgtatgcaag aaggcgtgaa attggaatta tgaaatacgt aggggctacg
     1441 gactggtttg tcaggatgcc ctttataata gaaggtatag tagtagggat gattggagcg
     1501 gtggtatcta ctgtaatact gggcgtaggt tactattatt gctccggtct tgtgaaaaac
     1561 cagatgatag gctttatgtc catatcactc atacccttta atcagattat agtaagcatg
     1621 gtaatactat tgattattgt gggggttgta atcggagcag tggggagcct tatttcagta
     1681 agaaagttca ttaaggttta ggaggggtga aattgaataa aaggatagca gcagctctga
     1741 tagcacttat gctttcaata ccctgtgcta atgtatatgc aaaaaatccg actgaaatag
     1801 atgagcttca gagaaatcag gacaagctta atcagcagat aaagcagatg cagagcagca
     1861 ttaacaagat aaagactgag aaagtaagtg tggcaaaagc catagaagat ttggacagca
     1921 agcttaatca ggctgaggat gagcttaccg gtgttgaaaa ccagctttcc cagttggaga
     1981 accagatagc agtgaccaca agggagcttg aaagggcttc aagtgaagct tcaaaccaaa
     2041 aagaactgct gaaaaagaga gtcagggtaa tgtatgaaaa cggaaatgtc ggatatatgt
     2101 ctgtaatact gaattctgca agcttctccg atttcatatc cagagtggat tttttgaaaa
     2161 aaatcatcag cttcgacatg aatctgctga gcaaaatgaa aagctaccgg gattcggttg
     2221 cagataaaag aagccagctg gcatcagagc tggaagagaa agaaaggctt aagaacgaaa
     2281 taggtaagaa aaaggaagag gttgagacag caaagcaggt caaagagatg accctgaaag
     2341 agatactcag tgacctcaag gaactggagc gccaggagga taagcttctg gagcagtcta
     2401 aagagattgc aaaaaagata gttgaactgc agtcaaagga aaagtatatc ggaggagagc
     2461 tgacctggcc ggcaccgggt tattacaaga ttacatcaca atatgggaat agaactcatc
     2521 cgatacttaa gaaaaagaag atgcatacgg ggatagatat cgcagttccc agcggtactt
     2581 ccgtaattgc cgccaatgca ggaaaggtca tatactccgg gtacaacgga ggatatggga
     2641 ataccgtaat aatagaccat gggggaaaaa tatccaccct ttatgcccat aacagcaagc
     2701 tccttgtaaa agtaggagac aaggtcgaaa agggccagac aatatccaag tccggcagta
     2761 ccggactttc cacaggcccg cacctgcact ttgaagtaag ggaaaacggc cagcatgtgg
     2821 atcccatgaa atacctgaca ggcaaaaagt agggtcacca tacatggtga cagaagcaaa
     2881 aatacaccga aaggtgtatt tttttgcata tttgcataat tgacacagcc ttgggcatat
     2941 tatacaatgt aaatgcaaat gatttgcaaa aaggagcaga ggcaatgaaa aaggcaataa
     3001 ctgcagtaat tattttacta ttgtcagcca tggcgctgtc aggctgtatg gaatccggaa
     3061 agaagccggc tgaccaaaga ttgaaaatat atgtcagctt ttaccctatg tattttcttg
     3121 caagtgaaat agcaggggac aaggcggaag tcataaccat ggtacctgcg ggagcggaac
     3181 ctcatgactg ggagcctgct ccaaagacgg cagcggagat cagcaaggct gatatgctga
     3241 tatataacgg ggcgggtatg gaaccatggt tagataaaat actgcccaat attaacgaaa
     3301 aaagaaccag attagttgat gccagtcagg gcatagagct tttgaagtct tcggagcacg
     3361 acgaggaaga agaagacata gaggaaaacc atgagcatgg aatctacgat ccccatatat
     3421 gggtaagccc cataagattc aggcagcaag cccggaatgt ttatgaggcg cttttggaaa
     3481 ttgatcctga gaattcggag tactatggaa aaaggaagat agaactggat tcaaggcttt
     3541 ccgatttggg caaagatata tgggatgctt caaaacattt caaaagcaat acaatcgttg
     3601 tatcccatga gtcctttggt tactttgcaa atgatttttt attggagcag attgccataa
     3661 gaggaatcaa tcctcaggag gaaccaagcc cctcaaaaat ggttgagctt atcagaaaat
     3721 gcagggagaa gaaagtaaag tatgtttttt ttgagaagct tacaagccca aagctctcag
     3781 agaccctggc aagagaagtg ggagcagggg tcctggtgct taatgatgcg gcaggcctca
     3841 gtgaaaagga tataaagtcc gggaaggatt atataactgt aatgtatgaa aatcttgaaa
     3901 acctgaaaaa ggcattgggt gattaaatgg gagcagaaat aataagagtt gaaaacataa
     3961 ccttcggata cggtaaagcc aaggtgctgg ataatgtcag tacaagcgtt attgacggag
     4021 attttatagg gattgtaggc ccgaatggct cgggtaaaag tacgctgctc aggatttttc
     4081 tcggtttgat aaaacctcag aaaggtactg tcagggtttt tgataccgat atagaaaaat
     4141 tcaaccaatg gggcaagata ggatacatac cgcaaaaagc cacttccttc aaccagggct
     4201 tccctgcaac agtggaagag gtggtttcgg caaacctata tcccgttgta ggcttggcag
     4261 gacgcataaa aaagactcat tgtgaaaagg ttgatgaggc acttcagatt gtagggatgc
     4321 tggagcaaaa gaaaagcatg gtcggcaggc tttcaggagg gcagcagcag agggtattta
     4381 ttgccaaagc cctggtcagc gctccccggg tgatattgat ggatgagccg acggtaggca
     4441 ttgatcagaa ttctgaagaa agcttttatg aattgatggg attcctgaac agggaaaagg
     4501 gcatcacact ggtaatggta acccatgata tcggtgccat agacaacaga gtaaacaggg
     4561 tattgtgctt atccggcgga aagctttacg agcatgactg tgcagtgagc cgggcttcgg
     4621 atatattcaa ggaggcatac ggaaagcgcg atagcttgcc gataatacac agacactagg
     4681 taataatgga ggagtataac ttatgtttga attattcaat tatgaattca tgcagagggc
     4741 tcttgcagca ggattgctgg tggctgtgat ctgccccctt ataggtactt ttgttgtaat
     4801 gagaagattg tctatgatcg gtgacacact ctcccatgct tccctggcgg ggattgccgc
     4861 cggcatgctc ggtgggcttt atcccttatg gagtgcactg attttttctt tagccgctgc
     4921 aataggaatt gaaaaattga gaaagcactt tccgcaatat gcggagcttt cgatatccat
     4981 agtgctttcg gcaagtatag ggctggctgt tgtacttatc agccttgcca attcctttaa
     5041 tgcggatctt atgagttatc tgttcggaag cataatagcg gtgaatgcac ttgatatata
     5101 cataattctg ggcttaagca ttatcatact ggtatcggta gggcttttct acaaggaatt
     5161 cttctacatg gcctttgatg aggagggcgc agaattggcg ggaatgccgg tatcaagcct
     5221 taatatgtat tttacggttt tgacggccat gactattgtt gtctccatga gagttgtcgg
     5281 catgatgatg gtatcctctt tgatggtagt cccggtagcc tgcagtttat tgatatcaaa
     5341 aagctttaag aatacgattt ttttgtcggt attctttgca ttgatttctg tagttatagg
     5401 gcttttttca tcctattggt tcgacctggc gccgggaggt tccattgtac tgacctcagt
     5461 atttatactt attgctgtaa taattgttaa aagaatattc aggatgcagt gattctgcgg
     5521 agctgaccaa ggaagtgcga aaatgaagga tattgaaaag ctggaaaccg aattgaagga
     5581 gaagggctat aagcttacgg agcagagaag gtccattatt gaggctttta atgaaaaccc
     5641 cgggcatcac actgctcaaa agatatttga gctggtaaag agcaagctga agggaataaa
     5701 cttttcaacc atatatagga atcttgagct tctgagcact cttgaaatca tcagcaagct
     5761 gtatgatgaa agcggcatca gccattacga gcttttcgga agaacacacc atcatcatat
     5821 aatatgcaaa ggctgcggag agaccagaga gatggatatt tgcccatatg caggactgga
     5881 tgagggacag ctgagtgcaa tgggcttcaa ggcaacaagc cacaaatttg aaatctacgg
     5941 ttactgcagc aagtgcaggt tattaaagga gcaagattaa gagtaagtac aaattaatga
     6001 ttgagataat atatgaagga tgataataat ataataagaa gaggtaagaa ttctgatttt
     6061 tttatgaggt gaaattgatg ataaataaaa gggcagcaat tttctgggca ataatactgg
     6121 taatagtcac agcctttggc acttttgtcg ttacaaacac aattgctatc agcctgggga
     6181 acaaggtggt aatagctcag aaggattttg aagcgattaa gagtatggat aagctcatgg
     6241 ggcttaaaga gtatatcaag agcaattatg tggaaggcgc agagggcaat aagcttatag
     6301 agggtgcaat aaaaggcatg tttgagtcct tgggagaccc ctattctgtt tatatgacca
     6361 aggaggaatt caagaatttc aatgaaacga ccaaaggctc ctatggaggc atcggagtca
     6421 ttgtcagcag aagcgaggac ggctatgtga cggttgtggc tcccatagag gatacccccg
     6481 gagaaaaagc ggggctcaaa accaatgata aaattattaa ggtggacgat aaggatatag
     6541 tcggcattga cctggaagag gcagttgctc ttatgaaagg caaaaaaggc actaaggtta
     6601 cattgaccgt tatgagggat aatgttcggg agcccaagat attcgaaata accagggaag
     6661 aaattatcct caagacagtg aaaagcaata tgatggagaa tagtataggt tatattagaa
     6721 taaccatgtt tgatgaggat acaggaagtg agtttaagaa agctttgaaa gagcttactt
     6781 cacaaaacat gaagggtttg atattggatt tgaggcaaaa ccccggaggt tttataaacc
     6841 agtgcgttga tgtagccgat gagcttctgg atgaggggct tgtagtatat accgaagata
     6901 aggcgaagaa acgggaagat tataaatccg gcaagggtaa actggaagtg ccctttgtag
     6961 tgttgataga cgaaggcagt gccagtgcct ctgaaatagt ttcaggtgcg gtaaaagaca
     7021 ggaaagcagg gcttcttatc ggtgtgaaaa cctttggcaa aggattggtg caatccattg
     7081 agcagctcaa agacggttcc ggcataaagc ttacgaccca gaagtactat acacccaatg
     7141 gcataagcat aaacaagata ggcatacagc cggatattga ggttaaagcc ccggagccgg
     7201 aagagggaca gaaccctgag gatgtgaagg atgtacagat ggaaagggct gtagcggaac
     7261 tgcttaagca aataaagtaa tttgcggggt gctttatgtt tacggtgctt aagataatgg
     7321 ggctgatatt tggacaattc ggcagctttc tgatgaatga attattctgg ctcatagcgc
     7381 tgatagtgat attgatttac aggaagaatt cggatattga agcgaagatg cttggcagca
     7441 gttataagct gcattacaag gtggcaggct cttttatcgt cggattgacg gcaggcctta
     7501 taggaagcct gattgtaacg cttgtgggta tcagcataga ggattatacc aggtcgggag
     7561 gaggctccct gatagaaggc attacttata tatgggcggt tgctatcctg ctttctgcaa
     7621 tcaatcccag atacctgtgc ttttcctatg caggaggaat tattgccctg atgaacctgg
     7681 tgttcgggtt tccgtcggta aatgtcccgg gcctgatggc actcattgga gtactgcacc
     7741 tggttgaaag ccttcttata tggatggacg gcagcagcta ttccgtccct ttatttttaa
     7801 gagggaagga cgggaaaacc gtcggcggtt atatgatgag cagaatttgg cctattcccc
     7861 tggtggcttt tgctgtgata ctcggaattg aaacaggcgg agtgagcctt gggggtataa
     7921 ttgacatgcc cggatggtgg cccttattaa agcattccgc cgcggggagc cgtcacggat
     7981 tggcatatct gccccttgta gtgcctgtag ttctgggcta cggggatttg gccttaacga
     8041 gaactcctga acaaaaatgc aggaattctg ctttaaggct tgcaggctac agcctggtgc
     8101 ttatcacatt gtcggtgatt gcttcaaagc tcaggctttt tgcatttgca gccgcagtat
     8161 tcgctcctgt tgctcatgaa atgctcattc aatacggggt aaaggaggag gaagagggca
     8221 agccatattt tgcaaacaaa gagacaggcc tgagagtgct ttatgtcaaa aagggttccg
     8281 ttgcatgcaa aatgggaatt gtgcccggag acacaatatt aaggatcaat ggaacagctc
     8341 tgctcagcga aaaacagttg gctgaatttt tagcctcctg ccctgttttt atctgggtgg
     8401 agtttgagaa gtacgaaggt gaattgatta ctgccgagta ctcggactac aggaacggaa
     8461 tcggagattt ggggatactg attgtccctc ataatgcgaa attgtattat gagataaaca
     8521 ggagcagttc ccttgccaag aggtttatta aaaggtttat ttcaaaccgg aacagggata
     8581 ttgatatgta acggatgaag tcatggtttt cgaacctctt ttgtttttaa ttgtataatt
     8641 cagaagctca atgaaagcat cgcggtttct ggcatttgca ttttcaaaat aattgaaaac
     8701 ctttataaat tcgttgtttt cttctttcac atccatacct cccatggaca ttttttatga
     8761 atttctacaa aaataccccc atacattcat tatttctccc aatggaatta atattcccga
     8821 tgaaatatac ttaatttatc aaatagcaag ggagagaatt gaattggcaa atctgtcctt
     8881 gaaacatgtg aaaaaggtat acccaggaaa tgtgacggcg gtgaaggatt tcaacctgga
     8941 cattgaagat aaggagttca ttgttcttgt aggaccctcg ggctgcggaa agacaacaac
     9001 cctgaggatg atagcggggc ttgaggagat tacggaaggg gagctttaca tcggcgacag
     9061 gcttgtaaat gatgtagcgc caaaagacag ggatatagcc atggtatttc agaactatgc
     9121 cttatatccc catatgaccg tttatgataa tatggccttt ggcttgaagc ttaaaaagat
     9181 tccaaaggct gagatagacg aaagagtcag gaaggctgca aagatacttg aaatagagcc
     9241 gctgctggac agaaggccca aggcattatc gggtgggcag agacaaagag tcgccctggg
     9301 aagggcgatg gtcagggagc ctaaagtgtt cctcatggat gagcctcttt cgaaccttga
     9361 tgcgaagctg agggttcaga tgaggactga gatagcaaag cttcataaga ggctgaacac
     9421 cacctttata tatgtaaccc atgaccagac agaagccatg acaatgggaa ccagaattgt
     9481 tataatgaag gacggggata tacagcagat agacagtccc cagaaggcat atgacaaacc
     9541 ggccaatcta tttgttgcag gcttcatagg cagccctcag atgaatgtct tcaggggcaa
     9601 tgttgttgaa gcggggggca aggccgcttt ggagcttcct gacggaactg ccctgggttt
     9661 gacggatgat gcggcaacaa ggcttaaaga ttccggctat gtttcaaagg aagtatatgt
     9721 aggcatcagg cctgagcatc tgcatgatgc agaggttgat ccattgacga aggattattc
     9781 tgttattacc gctaaggtag aggtagcgga gcttatgggc tccgaaacct acctgcattt
     9841 tacaaaatgc ggcgtacagg caattgccag agttgacgca aagacctcca tgagggcagg
     9901 ggatactgcc gaggtgcatc tgcattggga taagatacat ctgtttgaca tagagactga
     9961 aaaggcaata taaaaatata tataaatatg ataaactata agcggagggt tttacccttc
    10021 gcttatagtt ctatcagagt caagtcgagg tatgcatatg gctgatctgc aaaggctgct
    10081 tgcacaggcg gcaaggataa ctgaaaagcg tatggctgtt attacaccga atgaaaggtt
    10141 ggcctggccg gaagattccg acatagatat aacctttgaa gaagcatgca aaaattcggg
    10201 gaaaaacttc aggggctgga atatatacat gccggaagat gcgggaacca ggtttttcct
    10261 ttgcatcagc gcggacagtt actatgaatc ggaggtcggc aaactggtaa tgctgcttct
    10321 aaattcagcc tttgaaaagg atgattccgc atcggcttat ctgaaaaggg ctttggaagg
    10381 caatctggat gccgccgggc tttcaatgct tgaggaaaac ttgaaaagct gcctcccggg
    10441 gtatgtactg ctgattgaca atattgaaaa ctcaagggat gagattattg agatcctgga
    10501 aaatacaatg gatattaaaa cagaacttat atatgaaaac aggataatcg ccgtggcgga
    10561 agccgaaaat atcacagagt catgcagcag ctttataaaa aatgtattaa gcgaactgct
    10621 tattgaatgc aatgcagtca taggaggaag ggccgcaaaa gcttcggagc tttaccccct
    10681 gtacaggaac tgcatggagg cgctgtccct caaacatata tacggacttg gggacaatgt
    10741 ccttgattat gacggcatgt atgggtacag ggtggcatac aatctggatc caaggcttaa
    10801 ggaatttttg agaagaaaag tgtttacggc tgaattcagt gatatggtaa aaggagaact
    10861 ggggattacc atagaggagt ttttcaggaa caatctcaac ctgacagaca cggcggccaa
    10921 gctgtatata cacaggaaca cactgcttta caggcttgaa aagatataca agtgtacagg
    10981 gtttgacctt aagaggtttg aggacagctg gcttttcagg ctcgcatgga tagtatccag
    11041 ggagagatag aagaggcttc ggaggaggcc ttttttgttg ttgacatgca gtagtattag
    11101 tattaatatt attacgaaca catgttctaa tgggtacgta gaagtattat tatgtaaaca
    11161 taatacttct gtgatataat tcttatatat attttgcaag aaggtgttat tatggacaga
    11221 ttcaagcttg tatcggacta taagcccacg ggggaccagc cggaggcaat agaaaagctg
    11281 gcgaaaggaa tactggcagg agaaaagtat cagacgctgc ttggggtaac aggctcagga
    11341 aagaccttta cagttgccaa tgtgattgaa aaggttcaga agccgaccct ggttcttgcc
    11401 cacaataaga ctctggctgc acaactctgc agcgagttca aggagttttt cccccacaac
    11461 tgtgtagagt attttgtcag ctattacgat ttttaccagc cggaggctta tgttgctcat
    11521 tccgatacat atatagaaaa ggaagctgat ataaatgatg agattgataa gctcagacac
    11581 tcggcaacag ctgcgctgct tgagagaaaa gatgtcatta tcgtagccag cgtatcctgc
    11641 atatacggcc ttggggatcc tgaggactac aggtctcttg tgctgtcctt gagaaaggga
    11701 atggtcaggg acagggatga ggtgctgaga aagcttattg atatccaata tgacagaaat
    11761 gatataaatt ttgtcagagg caccttcagg gtaagggggg atattgttga tattttccct
    11821 gccgcctcgt cggataaggc gataagagtt gaatatttcg gggatgaaat agaaagaatt
    11881 ctggagatag actctcttac cggaaatatt acgggagaac ggcagcatgt atatatttac
    11941 cctgcttccc acttcgccac tacccaggcc aagcttgagg ctgcaataag caatattgag
    12001 gcggagctta tagagagggt aaaggagctg aaggagcagg ataagctcct tgaagcccag
    12061 aggcttctgc agaggaccaa ttatgatatt gagatgatga aggaggtagg ctactgctcg
    12121 ggaatagaga attattcgag gcacataagc ggcagggcgc cgggaagctc acccttcacc
    12181 ctgctggact tctttccgaa ggattatctg ttgatagttg atgaatccca tgtcacaata
    12241 ccacaggtca ggggcatgta tttcggggac aggtcaagga aggagtccct tgtggattac
    12301 ggcttcaggc tgccgtcggc atttgacaac aggcccttga attttgaaga gtttgagcag
    12361 aggcttaacc aggtgattta tgtcagtgcc acaccggcga agttcgagct tgagcgaagc
    12421 acctgcgtgg ctgaacagat tataaggcct accggattgg tggaccccga gatagaaatc
    12481 cggccggtaa aggggcagat agaccacctg atcggtgagg tgagggagag ggctgcgaat
    12541 aaccagagag tgctggttac caccctcacc aagaagatgg ctgaggatct gactgcttat
    12601 atgaaggaac tggatatcag ggtgaggtat ctgcattcgg acattctcac cttagagaga
    12661 atgaagataa taagaagcct caggctgggt gaatttgacg ttcttgtggg cataaacctg
    12721 ctcagggagg ggttggacct tcccgaagta tcccttgttg ccatacttga tgccgataag
    12781 gaaggattcc taaggtcgga gatatccctt atccagacgg tggggagggc tgcaaggaat
    12841 gtggacggaa aggtaataat gtatgccgac aatattaccg attccatgag aagggccata
    12901 agtgagacca acagaagaag aaagattcag atggattata acgaaaagca cgatataaag
    12961 ccccagacta tcagaaaggc gatacacgaa atcattgaag ccactactgc cgcagaagag
    13021 tccgcaaaat acggcattaa gcatgatatt gaattcatga ggaaggatga tttgctcaga
    13081 ctgattgccg ctttggagga agaaatgaag gactgtgcga gagcattgca gtatgaacgg
    13141 gctgcggaac ttagggataa aataattgaa ttaaggcata ggctgaagaa ataacgaggt
    13201 gtataatggc aaaggataaa attattatta agggtgcccg ggagcacaac cttaagaata
    13261 tagatgttga aataccaaga gataaatttg tagtaattac agggctaagc ggttccggta
    13321 aatcctccct ggcttttgat accatatatg cggaggggca gaggcgctat gtagagtctc
    13381 tttctgctta tgcaaggcag ttccttggtc agatggaaaa gccggatctg gattatattg
    13441 agggcctctc tcccgcaatt tccatagatc agaagacaac caacaggaat ccccggtcaa
    13501 cggtgggcac tgtaacggag atatatgact atttgaggct gctttatgcc agaataggga
    13561 caccccactg ccatgtgtgc ggcaaggaaa tatcccagca gacggtggat cagatggttg
    13621 actatataaa tacccttgaa ccgggaacca ggatacagct tcttgccccg gttattcagg
    13681 gcaaaaaggg agaacatcag aagctcctgg aggaaataaa gaaaagcggc tacgtcagag
    13741 caagaattga cggggagatt gccgacctca atgaggaaat caagctggaa aagaacaaga
    13801 aacatacaat agaagtaatc gttgacagaa tcatagtcaa ggaaggcgta aacaagaggc
    13861 tgacggattc cctggaaacc gtgctgaagc tttccggggg aacggtgctt atcgatataa
    13921 tcggcaagga ggaacgtctg ttcagccaga actttgcctg tgtggagtgc ggaataagca
    13981 tagaagatcc cgcacccagg atgttctcct tcaacagccc ctttgggaaa tgcccggttt
    14041 gcgacggcct tggtgaactt cggaggatag acccggattt ggttataccg gataagacaa
    14101 agtcggtcct ggaaggggca atagagccgt ggaagaatct tgatgagact tcatggtatt
    14161 acaatgtagt caatgcggcc gcaaaacact acggtttcag tatgaatacc cccatatgcg
    14221 aattggatga aaaagctgta cagattcttt tatacggcaa cggaggagag aagattaagg
    14281 taaagtatca gagagagtac ggcagcggcg aagtgatgat ggctttcgag ggtattgtga
    14341 acaaccttga aaggcgttat ttggagacaa agtccaacta catgaggtat gaaatagaaa
    14401 atttcatgtc ctctgtacca tgccccgagt gcaagggggc aaggcttagg aaggaaagcc
    14461 ttgcggttac tttaggcgga ttgtccattg ctgaattatg cagcctgtct gtatataaga
    14521 taatggagtt tctcgggagc ctgaagctta ccgaaaagca gcggattata tcaaagctgg
    14581 tgcttaaaga gctgcacgag agattggaat tcctggttac tgtcggcctt gaatacctta
    14641 ctctttcccg taccgcagga accttatccg ggggagaagc acagaggata aggctggcta
    14701 cccagatagg ctccagcctt gtaggagtat tatacatact tgacgagccc agcataggac
    14761 tgcatcagag ggacaacggc aagctcctga aggcccttcg gaacctcacg gatttaggga
    14821 atacgctgct ggttgttgag catgatgagg agacaatgca tgcagcggac tacatcattg
    14881 atatgggccc gggagcagga gcccacggag ggaagatagt tgcagccggc tcctttgata
    14941 ccatactggc ttgtgaagaa tcagttacgg gccagtatct gagcggaaaa aaacagattc
    15001 ccattccgca ggagcgaaga aaacccaatg ggaaatggat tgaaataaaa ggggcaaggg
    15061 agaataacct taaagacttg gatgctaata tacctctcgg ggtgtttacc tgcataacag
    15121 gagtctccgg ctcaggaaaa agcaccttaa tcaatgaaat actgtataag gagctttcac
    15181 acagattgta cggttccaag ctgagggctg gagagcataa atggatcaaa gggatagagc
    15241 atattgacaa ggttattgac attgaccagt cacccatagg aagaacgccc aggtccaacc
    15301 cggctactta tacaggggct ttcgatatta tacggggagt gtttgcagcc acgccggagg
    15361 cgaaaatgaa gggctacaat aacggcaggt tcagcttcaa tgtaaggggc ggaagatgtg
    15421 aggcctgcaa gggtgacggt ataatcaaaa ttgaaatgca gtttcttccg gatgtgtatg
    15481 ttccttgtga agtatgcaag gggaagagat acaacaggga gaccctggag gtacgctaca
    15541 agggcaaaaa catttctgaa gtacttaata tgactgtgga ggatgctctt gaatttttca
    15601 agaacatacc gagagtgcag aaaaagctgc agacattatt tgatgtgggg ctgggatata
    15661 taaaactggg acagccttct accgagctgt ccggcggtga ggctcagagg ataaagctgg
    15721 cttacgagct gtcaaagaga agcacaggaa agactgtata tatactggat gagcctacca
    15781 cagggcttca tattgccgac atacacaagc tgcttggcat acttgacagg cttgtagatt
    15841 caggcaacag cattatcgta attgaacaca acctggatgt aattaaaagg gcggattata
    15901 taatagactt ggggcctgaa gggggagaca ggggaggcac tgttattgcc caaggcaccc
    15961 ctgaggagat atgcaaggtt gaaacctcat acacgggcca gtttttgaag aagatactgg
    16021 ctaaatatta ggaggacacg gg
//
LOCUS       DAOOAB010000058        15707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_4882_length_15707_cov_17.292487, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000058 DAOOAB010000000
VERSION     DAOOAB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15707
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_15707_cov_17.292487"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..467)
                     /gene="mce"
                     /locus_tag="PKU88_10720"
     CDS             complement(48..467)
                     /gene="mce"
                     /locus_tag="PKU88_10720"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPX69785.1"
                     /translation="MKGGIQLVSKIDHIGIAVSNLDEAVKLYRDVLGLELHGTEVVPE
                     QKVKVAFLPVGDTEVELLESTSAEGPIAKFIEAKGQGIQHIAFRVDDIEAALEEMKAK
                     GMRLIDEKPRYGAGGAKIAFLHPKSTGGVLIELCERK"
     gene            complement(512..1462)
                     /gene="meaB"
                     /locus_tag="PKU88_10725"
     CDS             complement(512..1462)
                     /gene="meaB"
                     /locus_tag="PKU88_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPX69786.1"
                     /translation="MDIKAGLLNNDKRAAARLISMMENGEPEAVQIIKECYNMTGHAR
                     IIGITGPPGVGKSTVTDKLAKQLRKEGKQVGIVAVDPTSPFTGGAILGDRVRMADLNN
                     DPGVFIRSMGTRGALGGLSKAVHGAVKVLDIFGMDYIFIETVGVGQSEIDIVKLADTV
                     IMVMVPGLGDDIQAIKAGIMEIGDIFAVNKSDRDDAERTAAEIEAMLDFNRNEEYRPP
                     VLNIIARDNKGIEKLLELIEKHWEYENKSGSYKQKKLNRLRTEIMDIFRDEVMKTLYS
                     ISENTNIINELAQKVMDKQMDPYTAADRLMDEFTNNQRNN"
     gene            complement(1475..1873)
                     /locus_tag="PKU88_10730"
     CDS             complement(1475..1873)
                     /locus_tag="PKU88_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPX69787.1"
                     /translation="MDRPIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQTP
                     EQIAETAIQEDADCVAMSILSGAHNHLFPKVVNLLKERGADDILVVGGGVIPEDDIPG
                     LIEAGIAGIFTPGTPTSVTIDFIKNNIKRK"
     gene            complement(2053..3729)
                     /locus_tag="PKU88_10735"
     CDS             complement(2053..3729)
                     /locus_tag="PKU88_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPX69788.1"
                     /translation="MFNKEKLEQLKKAHEEWENTVTKAVAKRPERKKEFETSSGIAVK
                     RAYTPADIENLDYDRDLSNPGEYPYTRGVQPTMYRGKFWTMRQYAGFATAEESNKRYK
                     YLLEQGQTGLSVAFDLPTQIGYDSDHPLSEGEVGKVGVAIDSLKDMEILFDGIPLDKV
                     STSMTTNAPASVLLAMYIAVAEKQGVSSDQLSGTIQNDILKEYIARGTYIFPVEPSMR
                     LITNIFEYCSKHVPKWNTISISGYHIREAGSTAAQEVAFTLADGIAYVDAAVKAGLDV
                     DEFGPRLSFFFNSHNEILEEVAKFRAARRLWAKIMKERFKAGDPKSMMLRFHTQTAGC
                     TLTAQQPDNNVVRVAMQTLAAVLGGTQSLHTNSRDEALALPTEDSVRIALRTQQIVAY
                     ESGVTETADPLAGSYYIESLTDQIEQKAVEYLNKIDEIGGAAKAIEKGYIQKEIQDSA
                     YKYQKEIEAGERIVVGVNKFQIKESAPKGLLKVDPKVGELQQKKLAELRKIRDNTKVN
                     AALEKLGNAAKGEDNLMPFILDAVKAYATLGEICGVLRKVFGEYQQSIVL"
     gene            complement(4002..4406)
                     /locus_tag="PKU88_10740"
     CDS             complement(4002..4406)
                     /locus_tag="PKU88_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPX69789.1"
                     /translation="MDNNLKIGITAQVGKTVSVDDTAVKFGSGGVRVFATPMMVALME
                     NAALNAVDPHLGEDSATVGLSLNVKHLAATPVGMKVTAAAELIGIEGKKLTFKVEAYD
                     EQEKIGEGIHERYIIKLSKFLERAAQKTSKVL"
     gene            complement(4483..6291)
                     /gene="ftsH"
                     /locus_tag="PKU88_10745"
     CDS             complement(4483..6291)
                     /gene="ftsH"
                     /locus_tag="PKU88_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPX69790.1"
                     /translation="MKKFLRGISFYILIIIIIYMFVLIIQNAEPAKDKLSYTQLVTEI
                     ENGRIKEVTVSDNNGMSVVTGKISNTRRFEAIVPSSEFNELVQDYIERDRLDVKYEIK
                     TAAAAWFSILPSIVLIVIFIVFFFVLTQQSQGGGSRVMSFGKSRAKLHTEDKKKITFA
                     DVAGADEEKEELGEIVDFLKQPRRFIELGARIPKGILLVGPPGTGKTYLARAVAGEAG
                     VPFFTISGSDFVEMFVGVGASRVRDLFEQAKKNSPCIIFIDEIDAVGRHRGAGLGGGH
                     DEREQTLNQLLVEMDGFSVNEGIIIIAATNRPDILDPALLRPGRFDRQITVGIPDVKA
                     REEILKIHARNKPISLDVNMETLAKGTTGFTPADLENVMNEAALLAARKGKKKIEVID
                     LDEATIRVIAGPEKKSKVVSEHDKKLTAYHEAGHAVVQKLLPNADPVHQISIIPRGRA
                     GGYTISLPQQDKFYMSKSELEEQIVSLLGGRVAEKLVLDDVSTGAKNDIERATGIARK
                     MITEYGMSDSLGPMTFGSEHDEVFIGKDLTRTRNYSEEVAAIIDKEIRKVIDAAYHKT
                     ETLLKENISKLHGVAQALLEREKLDQAEFEEVFASA"
     gene            complement(6525..7067)
                     /gene="hpt"
                     /locus_tag="PKU88_10750"
     CDS             complement(6525..7067)
                     /gene="hpt"
                     /locus_tag="PKU88_10750"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPX69791.1"
                     /translation="MLEDMKSILISEEEIAKRVKELGKQLAEDYKGKELLVVGILKGC
                     MLFLSDLVRTIDLPLTLDFMVVSSYGATTKSSGVVRIVKDLEREIEGKDVLIVEDIVD
                     TGLTLSYLVENFKARNPKSVKVCSLLDKPDRRKAQVEIQYIGFKIPDEFVVGYGLDYG
                     ENYRNLPFVCVLKPEVYEKK"
     gene            complement(7072..8460)
                     /gene="tilS"
                     /locus_tag="PKU88_10755"
     CDS             complement(7072..8460)
                     /gene="tilS"
                     /locus_tag="PKU88_10755"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPX69792.1"
                     /translation="MTGFIENVRKTIAKYDMVKPGDKIVIGVSGGPDSLCLLHVLKGL
                     CREYGCSIFAAHLNHKFRGKAADSDAEFVEKICREWDIPVFIEVFDVPAYIKETGLSP
                     EEAGREIRYRLFRRVCEEVSGNKIAVAHNLNDHIETILMRFMRGSGIEGLKGIEAVRG
                     NIIRPLLETDRLSIEDYCAFYNLNPRIDKTNLESVYNRNKIRLELIPYMEKNFNPNIK
                     MALSRFSGLIKDENDFLETEAREKFKEVAEDSDDRVVIEVTKFIALHKALQRRIIRQC
                     AERLLNTLNGFEFKHFEKVLEIAHQSTGAAVMLPHKLKAFRSYDKLVLAKNIVKADKK
                     CYYKLKYDYDNSVDTDNGRITVQRIKAKEIAELKGQKDTIYIDPLKIKGELVLRCRQA
                     GDMFAPIGMKGTKKLKEYLIDEKVPREKRDDLELIADGHEIVWIVGGRLSEKYKISDD
                     TSDAVLIKYIRG"
     gene            complement(8684..9391)
                     /locus_tag="PKU88_10760"
     CDS             complement(8684..9391)
                     /locus_tag="PKU88_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPX69793.1"
                     /translation="MDAWIDSGEIKQIIIVTDGKSGSGISLVEAAKEAYDKGIVVSAI
                     GIVDPEAGSESEAEEAKKIAEAGGGLWYYSKAEDLKYAVKEIAIDTSVKTIERIVDRQ
                     LKAIIGEGIPDLEPESRTKIMNFIRKYGGNINLKCIVVIDAGRIIKGGFTIAEKSAAG
                     LLESIRIRKGCSSIAVIDCSGETAGIHDTICEFAGDISLLNRNPELICCSGDGQAGAA
                     ILKACELMNQYYEVYNA"
     gene            complement(9484..11871)
                     /gene="spoIIE"
                     /locus_tag="PKU88_10765"
     CDS             complement(9484..11871)
                     /gene="spoIIE"
                     /locus_tag="PKU88_10765"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HPX69794.1"
                     /translation="MVVEVATVSNQSVTNKRGRIRHIIDFFMNTSFRMYMILSAFGLL
                     LGRAVILDFLNPFCTAFFAAAMVQKLNLFVMGTSILLGVLSVGDKGILLKYIIAAIVL
                     IIPYAATRKMKVNRKLVISAAAALINLAAGYFIFYVQNYYLYDLLMLIIESILIAILI
                     YIYDRSLPVIKNYKNRRLLSSEEVVAVAVMAAFCFVGTEIYIAGFSVKNIAVIFIIMI
                     FAYLGNVGTGAAVGIIMGIVQALSGSILPSAIGVYGLCAMMCGILKNLGRYGCPLGFI
                     ISNALMTFYINGSTEVLIRFYEILIASFLFVCIPQNYMKKLSGYKLSIAGEYLKEKSY
                     SMRIKENTIERLSEFAGVYKSLADTLKDTIQDRSYFSHTDAAQIIDQVVGKTCSDCGL
                     AGSCWKRNFYNSYQYLFNLLTIIENGGKLKKDGCYKGFKERCLKPEEIIENLKYYYDM
                     YRNGLGWKKKINESRLLVSDQLKEVSAVVSDLALRIDMDVDFDRDMEELIMVGLDNDA
                     VRVKDVIVARTGNTMDVDIRVDSCGGKRECIKNIIPIVNRVTGKKFNKREMTCDMIHG
                     SICSIKLKEAQRYQIATGIARMNKSGAISGDNYSFIELKDGKFMLALSDGMGTGPKAA
                     LESSTTITLLEKFLYAGFDKDVALKAINSLMLLKSNDETYSTVDMTVINQYNGEVEFI
                     KVGAVSTFIKNEEGVQIIKTGTLPVGILSRIDVELVRKKLKDGDFVVMVTDGVLDCNR
                     EIVDKEKWLAELIRDVDTRNPQRLAEEILQSCLQANGGEAPDDMTVMAAKVWEAM"
     gene            complement(12060..12136)
                     /locus_tag="PKU88_10770"
     tRNA            complement(12060..12136)
                     /locus_tag="PKU88_10770"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12100..12102),aa:Met,seq:cat)
     gene            complement(12150..12225)
                     /locus_tag="PKU88_10775"
     tRNA            complement(12150..12225)
                     /locus_tag="PKU88_10775"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12190..12192),aa:Met,seq:cat)
     gene            complement(12233..12321)
                     /locus_tag="PKU88_10780"
     tRNA            complement(12233..12321)
                     /locus_tag="PKU88_10780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12285..12287),aa:Leu,seq:taa)
     gene            complement(12435..13358)
                     /locus_tag="PKU88_10785"
     CDS             complement(12435..13358)
                     /locus_tag="PKU88_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HPX69795.1"
                     /translation="MKRIAVIDIGTNSMRLMLCKIEGNFIANKEKELMITRIGEDVSK
                     TGMITEKSFARNIEALKYFRNKADRYGAEEVYAIATSAVRDALNGGAFVADAKSQAGV
                     DVMIISGDEEAELGLRGVMSEIQNPQETVLVIDIGGGSTELVLGSKGKTDYKVSIPAG
                     AVRMTEHFVTGNPIRNEDVENMKNRLNEIFKEPLEHLGKKRIDKVVAIGGTATTIAAM
                     FHNMSIYDPETVHNTIVDSEYIESIFNKLKDMSVEERWNVRGLQKERADVMPSGIFIL
                     QHIIEGLKRDSLVVSENDNLEGIIEKYVLSK"
     gene            complement(13495..13920)
                     /locus_tag="PKU88_10790"
     CDS             complement(13495..13920)
                     /locus_tag="PKU88_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HPX69796.1"
                     /translation="MPIEEGKVVEGIVSSITSFGAFVQLPEGKMGLVHISEIADTYVK
                     DIKHFIKEKDKVMVKVLSVEKNGKINLSIKQAQEIKKTNRPIEIDWEQEARKNQGISF
                     EDRLSKFLKESDEKLQQLKKNTDSKRNGGYRKNDGLQKG"
     gene            complement(14060..14347)
                     /locus_tag="PKU88_10795"
     CDS             complement(14060..14347)
                     /locus_tag="PKU88_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPX69797.1"
                     /translation="MKKRKLRLKNIFMIAFIVYAAITIVNQQLTIYDLRKSEQDEISK
                     IEAIRNDNDRLMEMINNATSVEYIEKLAREQLGLVKPGEKVYIDQSSDEKE"
     gene            complement(14496..15035)
                     /locus_tag="PKU88_10800"
     CDS             complement(14496..15035)
                     /locus_tag="PKU88_10800"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HPX69798.1"
                     /translation="METVQFQSYVFLYSLYGGIIIGILYDIYRVIRGRKKTERLITSL
                     WDILFLFSVFAVVIWAIFSSSYGEIRSYVLIGFLVGFYLYEKLLGRIASGLFHLLYKS
                     ISSFFMKTGSILALPLKMACSFVKSCCCSLIRILGKKKIRLRKVCGLPKQVIYDSKKY
                     YRLIVKRKERKENSNKGFS"
     gene            complement(15297..15584)
                     /gene="yabP"
                     /locus_tag="PKU88_10805"
     CDS             complement(15297..15584)
                     /gene="yabP"
                     /locus_tag="PKU88_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HPX69799.1"
                     /translation="MEEKKLIKSKPHSITIEGRERLIINGVRDVASFNENEVSLETEA
                     GGLVIRGTDLHINRLNVDDGNLFIEGYIISCVYNDKIDIKKTGSFLSNIFK"
BASE COUNT         3970 a         3994 c         2638 g         5105 t
ORIGIN      
        1 ttgattttat ctattgacat agaatctcct ccactttctt aatgctctta ctttctctca
       61 caaagctcta ttaaaacacc gccggtactc ttgggatgaa ggaatgctat tttcgcaccg
      121 ccggccccat atctcggttt ttcatcaatg agcctcatcc cttttgcttt catctcttcc
      181 agtgctgcct caatatcatc cactctaaag gctatatgct gtatcccctg gcctttagct
      241 tcgataaact tcgcgattgg cccttcggcc gatgtcgatt ccaatagctc tacctcagta
      301 tcccctaccg gcaggaatgc cacttttact ttttgctcag gaactacttc agttccatgg
      361 agttccagtc ccagtacatc cctgtaaagc ttcaccgctt catcaagatt actgactgct
      421 atacctatat ggtctatctt gcttaccaat tgaatacctc ctttcatcta ttatctttaa
      481 tacttcttcg aataatatat atataatacc tttaattgtt cctttggtta tttgtaaatt
      541 catccattaa tctatctgca gcggtgtaag ggtccatctg cttatccatt accttctgcg
      601 ccaattcgtt aataatgttg gtattctcac ttatactata cagggttttc ataacttcgt
      661 ccctgaaaat atccattatc tctgttctta acctgttgag ttttttctgc ttgtagctcc
      721 cgcttttgtt ctcatattcc cagtgctttt caataagttc cagaagcttt tcaatgcctt
      781 tgttgtctct tgcaattata ttcaaaaccg ggggcctgta ttcttcattc cggttgaaat
      841 ccagcatggc ttctatttct gcagcagtac gttcagcatc atccctgtcg gatttattta
      901 ctgcaaaaat atctccaatt tccataatgc ccgccttgat agcttgtatg tcatctccca
      961 gccccggaac catgaccatt ataacggtat ccgcaagctt cacaatatca atttcagact
     1021 gtccaacgcc aactgtttca ataaatatgt agtccattcc aaaaatatcc agaaccttga
     1081 cagctccatg gactgccttt gacaggcctc ccaatgctcc ccttgtaccc atgcttctta
     1141 taaaaactcc ggggtcatta ttcaggtcag ccatccttac tctgtctcca agaattgccc
     1201 cgcctgtaaa agggcttgta ggatcaacag caactatacc cacctgtttg ccctctttcc
     1261 gaagctgctt ggcaagctta tcggttacag tactcttgcc gactcccggc ggcccggtaa
     1321 tacctataat cctggcatgg cctgtcatgt tgtagcactc tttgattatt tgaaccgctt
     1381 ccggttctcc gttctccatc attgatatca gtcttgcggc agccctcttg tcattattca
     1441 gcaagccggc ctttatatcc atatatatct cccgttactt tcttttaata ttgtttttaa
     1501 taaaatcaat cgttacggat gtaggagttc ccggtgtaaa tattcctgcg attcctgctt
     1561 ctataagccc gggaatatca tcctccggta ttacgcctcc gcctactacc agaatatcat
     1621 ctgcacccct ctccttaaga agattgacaa ccttcgggaa aaggtgattg tgcgctccgg
     1681 ataatatact cattgcaacg caatccgcat cctcctgtat agcagtctca gctatctgct
     1741 caggggtctg cctcaggcct gtatatatga cctccatacc tgcatctcta agtgctcttg
     1801 cgacaacctt tgcacccctg tcatgaccat caagccccgg ttttgcaact acaactctga
     1861 ttggtctatc caaattcaaa acctcccaac atatatcttc aaatttattg gctaaaattt
     1921 ttttgcctta ttcgtctatg agcctataca gttttgggtc tcgcggttcg tggctcacgg
     1981 ctcgtgtttc aaggaaaacc tccgaagccc tgcttgcacc cacgtgcctc gtaccacgcg
     2041 cctcgcgcca atttacagta ctatcgactg ctgatactct ccgaatacct tcctcagtac
     2101 tccgcagatt tcgccgagtg tagcatacgc ttttaccgca tccagaataa aaggcataag
     2161 gttgtcttcg ccctttgccg catttcccag tttctccaat gcggcattca ctttagtatt
     2221 atcccttatt tttcggagtt cggcaagttt cttttgttga agctctccaa cttttgggtc
     2281 aacctttaag agccctttcg gtgccgactc cttaatctgg aatttgttca cccctactac
     2341 tattctctct cctgcttcta tctccttctg gtacttatag gcgctgtcct gtatctcttt
     2401 ttgtatgtat cccttttcta tagcctttgc agctcctcca atctcatcta tcttgttcag
     2461 atattcaact gccttctgtt ctatttggtc tgtcaggctc tctatgtaat atgaaccggc
     2521 cagagggtct gctgtctctg taacaccgct ttcataagct acaatctgct gtgttcttaa
     2581 tgcgatcctc accgaatcct ctgttggaag agcaagagct tcatcccttg aatttgtgtg
     2641 cagggattgg gttcctccca atacagcagc caatgtctgc atagcaaccc tcacaacgtt
     2701 gttgtcgggc tgctgggcgg taagagtaca accggctgtc tgggtatgga accttaacat
     2761 cattgacttg ggatccccgg ctttgaacct ctccttcatt atcttggccc agagtcttct
     2821 ggccgctctg aatttggcca cctcttccag aatctcatta tgagagttga agaagaacga
     2881 cagtctgggt ccgaattcgt ccacatccag gcctgctttt accgcagcat caacatatgc
     2941 aatcccgtct gcaagggtaa atgccacttc ctgtgctgct gtcgaacccg cttctctgat
     3001 gtgatatccg cttattgata ttgtgttcca ttttggaacg tgttttgaac agtattcaaa
     3061 aatattggtt ataagcctca tggaaggctc taccgggaaa atatatgttc ctcttgctat
     3121 gtactctttc agaatatcat tctgtattgt gccagagagc tggtctgatg aaaccccctg
     3181 cttttccgca actgctatat acattgccag cagaactgaa gcaggagcat ttgttgtcat
     3241 tgatgtgctt accttatcaa gagggatacc gtcaaaaagt atttccatat ccttgaggga
     3301 atctattgca actccgacct ttcctacctc accttcagaa agcgggtggt cggaatcata
     3361 acctatctgt gtcgggaggt caaaagccac actaagccct gtctgtccct gctccaaaag
     3421 atatttatac ctcttgttgg attcctctgc agttgcaaag cctgcatact gtctcatggt
     3481 ccaaaacttt cccctgtaca tggtaggctg tactcctctt gtatagggat attccccggg
     3541 atttgacaaa tccctgtcat aatccaggtt ttctatgtct gcaggtgtat aagccctctt
     3601 taccgcaatg cctgaagacg tctcaaattc cttcttcctt tcaggccgtt tggcaactgc
     3661 ttttgtgaca gtgttttccc actcttcgtg ggcctttttc agctgttcca gcttctcctt
     3721 attgaacata tttatcctcc ttttattatt tgagcatatc cgatggaaaa tatcaacaaa
     3781 ggatattgca gcctgtcggt caatatcata ttgtgaaata aaaaattcat tatattatca
     3841 ttttgcaagt tttccttctt tattaatatt accatattat ttattaaatt gtgtagtatt
     3901 tttagataat ttttaagaaa ttctttgtta aaaaatagac agtgtacatg ctgtgaaaaa
     3961 gccttatcat tacaagaccg gttaaagcat ctgcttatgt attaaagcac ttttgaagtt
     4021 ttttgagcgg ctctttcaag aaatttagaa agcttgatta tatacctttc atggatgcct
     4081 tccccgattt tttcctgctc gtcataggct tcaaccttga aggtcagctt cttgccttct
     4141 atgccaatga gttcagcggc ggcagtgact ttcatgccaa caggggttgc agccagatgt
     4201 ttcacattca aactgagtcc tacagtggca gagtcttctc ccaagtgcgg atccactgca
     4261 ttaagtgccg cgttttccat taatgctacc atcatagggg ttgcaaaaac cctgactcct
     4321 ccgcttccga atttaactgc ggtatcatct acactaacgg tttttcccac ttgggctgtt
     4381 attccgatct taagattatt atccaatatg atcacactcc tatattattt agcttgtgcc
     4441 aaaatttaaa gcctagccct tagaaggcta agctttaata ttctatgcgc tggcaaacac
     4501 ttcttcaaat tcagcttgat caagcttttc tctctccagg agagcctggg caactccatg
     4561 aagcttgctg atattttctt ttaagagtgt ttccgtctta tgataagcag catctatgac
     4621 cttccttatt tccttgtcaa ttattgctgc cacctcttcg ctgtagttcc ttgtccttgt
     4681 aagatccttt ccgataaata cctcgtcatg ctccgatcca aaggtcattg ggccaagact
     4741 atcgctcatg ccgtattcgg ttatcatttt ccttgcgatg ccggtggctc tctctatatc
     4801 attctttgct cccgtactta cgtcgtcaag cacaagcttt tcagcaaccc ttcctccaag
     4861 aaggcttact atttgctctt ccagttcaga cttagacatg tagaatttat cctgctgcgg
     4921 caaagatatt gtataacccc cggcccttcc tctcgggata atggatatct gatgtacagg
     4981 atctgcattt ggaagcagct tttgtactac tgcatggcct gcttcatggt aggctgtaag
     5041 cttcttgtca tgctcactga ctaccttact cttcttttca ggccctgcaa tcactcgtat
     5101 tgtggcctcg tcaaggtcta ttacttcaat ctttttcttg ccttttcttg cagccaacag
     5161 cgccgcttca ttcattacat tttcaagatc tgcaggagta aagccggtag tgcctttggc
     5221 cagggtttcc atattaacat ccaatgaaat cggcttattc ctcgcatgta tcttcaatat
     5281 ctcttctctt gctttaacat cgggtatccc aacggtaatc tgtctgtcaa aacgtcccgg
     5341 cctcagaagc gccggatcca atatatcagg cctgttagtc gcagcaatga tgataattcc
     5401 ctcattaaca ctgaatccat ccatctcaac aagcagctgg tttaaggtct gctctctttc
     5461 gtcatgccct cctccaaggc ctgcacctct gtgtctgccc actgcatcaa tttcgtcaat
     5521 gaaaattatg cagggtgagt tttttttcgc ctgttcaaaa agatccctta ctctggatgc
     5581 accgacacca acaaacattt ccacaaaatc ggaaccgctt atggtaaaga acggtactcc
     5641 cgcttcaccg gcaactgccc tggcaagata cgtcttacct gttcccggag gccctacaag
     5701 aagaatgcct ttcggtattc ttgctcccag ttcaataaac cttctgggct gcttcaggaa
     5761 gtcaacaatc tctcccagtt cttccttttc ctcatctgcg ccagctacat cggcaaaggt
     5821 tattttcttt ttgtcttcgg tatgcagctt tgcccggctc tttccgaagg acataaccct
     5881 gctgcctccc ccctgggact gctgagtaag cacaaagaag aatactatga atataacaat
     5941 cagcactatt gacggcagta ttgaaaacca agccgccgct gctgtcttga tttcatattt
     6001 aacatccagc ctgtctctct ctatataatc ctgaaccagt tcattaaact cactgcttgg
     6061 aacaattgct tcaaaacgcc ttgtatttga aatcttgcct gtaacaacag acataccgtt
     6121 gttatccgat actgtgactt cttttatacg tccgttttct atttctgtaa ccaactgagt
     6181 ataggacagt ttatctttag caggttcagc attctgaatt atcaatacaa acatatatat
     6241 tatgatgata atcaatatat aaaagcttat acctcttaag aatttcttca acaataatcc
     6301 tcctttctat ataagtcaac aaacaacaca gatattttag cataccaaaa cttaaattac
     6361 aatatcgcag caggcaagcc aattgcctat catacacata agaagagaaa tgcgcaataa
     6421 ctgaagtttg cataaccctc cgcaacgcgg ggacatgggg acgtttcgcg tgtcccactg
     6481 gaagtgggac aggggaaacg tccccatgtc cccgtcgcat tattctactt tttctcatat
     6541 acctccggtt taagcacaca tacaaaagga agattcctgt aattctcccc ataatccagg
     6601 ccgtagccta cgacaaactc atcgggaatc ttgaacccta tatattgaat ttctacttgc
     6661 gcctttctcc tgtcaggctt gtcaagaagg ctgcaaacct tgacactctt aggattcctt
     6721 gccttgaaat tttctacgag gtaactcagc gtaaggcccg tatccacaat atcttctaca
     6781 ataagaacat ccttgccctc tatctctctt tccaaatcct ttactattct tacaacccct
     6841 gaggatttgg ttgtagcacc atagctggat accaccatga aatccaatgt cagaggcaga
     6901 tctatagttc ttaccaaatc agaaaggaac agcatgcagc ccttcagtat ccccactacc
     6961 agcagttctt ttcccttata atcttctgca agctgcttgc ctagttcctt aactcgtttt
     7021 gctatctcct cttcacttat caatatactt ttcatatctt ccagcaccgt attaccccct
     7081 tatgtatttt atcaaaacag catctgaagt atcatcagag attttatact tttcgctcaa
     7141 tcttccccca acaatccaga caatctcatg tccgtctgca ataagctcaa ggtcatctct
     7201 tttctctctc ggaactttct catctatcag gtattcctta agcttcttgg ttcctttcat
     7261 ccctatcgga gcaaacatgt ctccggcctg ccggcatctt aagacaagct ctccctttat
     7321 ctttaaagga tcaatgtaaa tagtgtcttt ttgcccttta agctctgcaa tttcctttgc
     7381 ctttatcctt tgcaccgtta tacggccgtt atccgtatca acactattgt cataatcata
     7441 ttttaactta taataacact ttttatcagc cttcacaata ttttttgcca gtacaagctt
     7501 atcgtaggat ctgaatgcct taagcttatg gggaagcatc acggcagctc ccgtggattg
     7561 atgtgcaatc tccagtactt tctcaaaatg tttgaattca aacccgttca aagtatttaa
     7621 aagccgctct gcacattgcc tgattatcct tctctgcaat gctttatgga gggcaatgaa
     7681 ttttgtcact tctataacca ctctgtcatc ggaatcctca gcaacctctt tgaacttttc
     7741 ccttgcttct gtctcaagaa aatcattctc atcctttatc agtccggaaa atctgcttaa
     7801 agccatttta atatttggat tgaaattctt ctccatatat ggaatcagtt caagccttat
     7861 cttgttcctg ttatatacag actccaggtt ggtcttgtca attctcgggt ttaagttata
     7921 gaaagcacag taatcttcta tgctgagcct gtctgtttca agcagtgggc gtatgatgtt
     7981 tccccttacg gcctcgattc cctttagtcc ttctattccg cttccccgca tgaatctcat
     8041 caatatcgtc tcaatatgat cgttcagatt atgcgcaact gcaatcttat tgccgcttac
     8101 ctcttcacat acccggcgga agagcctgta tctgatctcc ctccccgctt cctcgggaga
     8161 aagcccggtt tcctttatat atgccggcac gtcaaaaacc tctataaata ccgggatatc
     8221 ccattctctg cagatttttt ctacgaattc tgcatcgctg tctgctgcct tccccctgaa
     8281 cttatggttc agatgcgccg caaaaatgct gcagccgtat tccctgcaca gccccttcag
     8341 cacatgaagc aggcatagtg aatccgggcc tcctgataca ccgattacaa ttttgtctcc
     8401 gggcttcacc atatcatatt ttgctattgt ctttctgaca ttttcaataa agcctgtcat
     8461 aaatccaccc tcaatatgtt tatgtattaa tgctaccata cagccgcaac atttaaaagc
     8521 aaaaactaag aggacatggg gacgtttctc cgaggacacg gggacgtttc tcctgtcccg
     8581 ctttcagcgg gacaggagaa acgtccccgt gtcctcggag aaacgtcccc gtgtcctcgg
     8641 agaaacgtcc ccgtgtcctc ttatatttca atagtacaag gttctatgca ttgtatactt
     8701 cataatactg attcataagc tcgcaagctt ttagtattgc agctcctgcc tgcccgtcac
     8761 cgctgcagca tattagttcc ggattccggt tcaataggct tatgtcgccg gcaaattcac
     8821 atatggtgtc atgtattccg gcagtttcac ccgaacaatc tattactgct atactgctgc
     8881 agcctttcct tatcctgata ctctcaagca gtcccgctgc acttttctcc gcaatagtaa
     8941 aaccgccttt aatgattctg ccggcatcga taacaacaat acatttcagg ttgatgtttc
     9001 ccccgtattt cctaatgaaa ttcattatct tcgttctcga ttcgggctcc agatccggga
     9061 taccctctcc gataattgcc ttaagctgcc ggtctacaat ccgttctatt gtttttacgg
     9121 atgtatcaat ggcaatctcc tttactgcat atttcaaatc ttctgccttc gaataatacc
     9181 atagccctcc ccctgcctct gcaatcttct ttgcctcttc cgcttcactt tcactgccgg
     9241 cctcaggatc aacaatacct attgcgctta cgacaatacc tttatcatat gcttcctttg
     9301 cagcttcaac aagactgatt cccgaaccgg atttgccgtc tgttacaata attatctgtt
     9361 tgatttcacc gctgtcaatc catgcatcca taaaaatttc cctttcctgc aaatgtacga
     9421 atcttacaac taagtatatt cattattttg ccaaaaaagg atgccattat acaaagaaat
     9481 gctttacatt gcctcccaca cctttgctgc cattacagtc atatcatcgg gagcctctcc
     9541 gccgtttgcc tgcaggcagc tctggagtat ttcctccgcc agcctctggg gattcctggt
     9601 atccacatcc cttatcaatt ctgccagcca cttttcctta tcaacaattt ccctgttgca
     9661 gtccaatacc ccgtcagtaa ccattaccac aaagtcacca tccttaagct tcttcctgac
     9721 aagctccaca tcaattctgc tcagtattcc gaccggcaat gtaccagtct tgataatctg
     9781 cactccctct tcatttttga tgaatgtaga tacagcacct accttgatga attccacctc
     9841 cccgttgtac tggttaataa ctgtcatgtc cacagtcgaa tatgtttcgt cattggactt
     9901 cagaagcatc agcgaattga tggccttcaa agccacatcc ttgtcaaagc ccgcatatag
     9961 gaatttttca agaagggtta ttgttgtgct gctttccagt gctgcttttg ggcccgtccc
    10021 catgccgtcg ctcagggcaa gcatgaattt gccgtccttc agttctataa atgaataatt
    10081 gtctccggaa attgctcccg acttattcat ccttgcaatg cccgtagcta tttgatacct
    10141 ctgtgcttct ttcagtttga tgctgcatat gctgccgtgt atcatatcgc aggtcatctc
    10201 ccttttgttg aatttctttc cggttactct gtttacaatc ggaatgatgt tcttgatgca
    10261 ttcccgcttc cctccgcagc tgtctactct tatatctaca tccattgtgt ttcccgttct
    10321 tgccactatc acatctttta ctctcaccgc atcattgtcc aatcctacca taatcagttc
    10381 ctccatatcc ctgtcaaaat caacatccat atctatcctt aaagctaggt cggataccac
    10441 tgcggatacc tccttcaact gatcacttac aagcaatctg ctttcattta tttttttctt
    10501 ccagccaagc ccgtttctgt acatatcata gtaatatttg aggttttcaa taatttcctc
    10561 cggcttcagg cagcgttcct tgaagccttt atagcagccg tcctttttga gtttaccccc
    10621 gttttcaata atagtcagca agttgaaaag atactgatag ctattgtaaa agttcctctt
    10681 ccagcagctg cctgccaatc cgcagtctga acatgtcttc ccgactacct ggtctattat
    10741 ctgcgctgcg tcggtatggg aaaaatagct cctgtcctgt atggtatcct tcaatgtatc
    10801 agccagtgat ttatatactc ccgcaaactc agagagcctt tctatggtat tctcctttat
    10861 cctcatgctg taagactttt ctttgagata ctctcctgca atggatagtt tgtatcctga
    10921 cagctttttc atgtagtttt ggggaataca gacaaacaga aaggaagcaa tcaatatttc
    10981 ataaaacctt atcaatacct ctgtagagcc gtttatataa aaggtcatca gtgcgttgct
    11041 tataatgaag cccagggggc agccatacct gccgaggttc ttcaatattc cgcacatcat
    11101 cgcacaaagg ccgtaaacac ctatggcgga agggaggata gagcctgaca atgcctgaac
    11161 tattcccatg atgattccca ctgccgcacc cgttccgaca ttgccgagat aagcaaaaat
    11221 cataataatg aatatgacgg caatgttttt tactgaaaaa cccgcaatat atatctctgt
    11281 ccctacgaaa cagaaagctg ccattaccgc aacagcgaca acctcttcgg aggacagcag
    11341 ccttctgttc ttgtaattct ttatgacagg taaggaacga tcataaatgt atatgagtat
    11401 ggctattaaa atggattcta taatcaacat caaaagatca tagagataat aattctgaac
    11461 ataaaaaatg aaataccccg ctgcaaggtt aatcaaggct gcagcagctg atatcaccag
    11521 tttcctgttc actttcatct tacgggttgc tgcgtatgga attatcaaga caatagctgc
    11581 aataatatat ttcaaaagta tccccttatc tccgacgctc agcactccaa gcaatatgct
    11641 tgtccccatc acaaacagat tcagcttctg aaccatagcc gcggcaaaaa aagcagtgca
    11701 gaaagggtta agaaaatcca gaataaccgc tcttcccaat aacagcccga aggcactaag
    11761 tatcatatac attctgaagc tggtattcat gaaaaaatct atgatatgcc ttatccggcc
    11821 gcgtttgttt gtaacacttt gattagatac tgttgccact tcaaccacca tccaataatt
    11881 cttctaaacg tcgtataaag attctaacac aaggggtttg aataatttgt caggttaggt
    11941 gcttgaaaat ggaaaacttt ttgacaatgg agaggttttt ttgacatagg gtgtgcagta
    12001 tatggaatat gagacaaaaa ctaaaaaact cagacccaag tctgagtgtt ttggcgtttt
    12061 ggtagcggcg gtcagattcg aaccaacgac cgttcgggta tgaaccgaat gctctagcca
    12121 actgagctac gccgccatgt ataaaaaatt ggttgcgggg gagggacttg aacccacgac
    12181 ctccgggtta tgagcccgac gagctaccaa ctgctctacc ccgcgacgtt tgtggtgccg
    12241 aagaccggaa tcgaaccggt acggtcttta aggaccgcag gattttaagt cctgtgcgtc
    12301 tgccagttcc gccacttcgg cttaactgcc aatatcgata ccgcaaatta acggcaagta
    12361 ttattatatc gtctttgact ataaacgtca atacctaatt attgacatat cacttttgcc
    12421 actactcagc cggcttattt gctcaataca tatttttcaa taatcccttc gagattgtca
    12481 ttttcgctga cgaccagact gtctcttttc agtccttcga taatatgctg caatataaat
    12541 atgccggagg gcatcacatc agctctttcc ttttgaagcc ccctaacatt ccagcgctcc
    12601 tcaacagaca tgtccttaag cttgttaaat atgctctcta tatattcaga atctacaatc
    12661 gtattatgca ctgtttccgg atcatatatg ctcatattat ggaacattgc tgctattgta
    12721 gtggcagtgc cgccgatagc gactacctta tctattcttt ttttgccaag gtgctccaag
    12781 ggctctttaa aaatctcatt aagcctgttt ttcatatttt caacatcttc atttctgata
    12841 ggattgccgg taacaaaatg ctctgtcatc ctaaccgctc ctgcgggaat actgactttg
    12901 tagtctgtct ttcccttgct gccaagtacc agctcagtgc ttcctccgcc aatgtctatt
    12961 accagcacag tttcctgcgg gttctgaatc tcactcatta ctcctctgag tccaagctcc
    13021 gcctcttcat ctccgcttat tatcataaca tcgactcctg cctgactttt ggcatcggca
    13081 acaaaagctc ctccgttcaa tgcgtcgcga acagcactgg ttgctattgc gtatacctct
    13141 tcagctccat atctgtcggc tttattccta aaatatttca gagcttcaat attccttgca
    13201 aaggattttt ctgtaatcat ccccgtcttt gaaacatcct cgcctattct ggtaatcatg
    13261 agttcctttt ccttatttgc aatgaaattg ccttcaattt tgcaaagcat caacctcatt
    13321 gagtttgtgc caatatcaat aaccgctatt cttttcattt gcttctcctt ttattattcc
    13381 gttcatgcca aatttataaa caataaactg ccagacttgc cggcagttat ttacgcttat
    13441 tattatatat gattaaataa ttgcagtttg aattaagccc tattgttaag ttgattatcc
    13501 tttttgcagc ccatcgtttt tcctgtaccc gccattacgt tttgagtcag tatttttttt
    13561 aagctgttga agtttttcgt cgctttcctt caggaacttc gaaagccggt cttcaaatga
    13621 aataccctga ttttttcttg cttcctgctc ccagtctatt tcaataggtc tgttggtctt
    13681 tttaatttcc tgtgcctgtt ttattgaaag atttatcttc ccgttttttt caacggaaag
    13741 tactttaacc attaccttat ccttttcttt gataaagtgt tttatatcct taacataagt
    13801 atctgcaatt tctgatatat ggaccagacc cattttaccc tccggcagct gaacaaaggc
    13861 tccaaagctt gtaatgcttg aaacaatacc ttcaactacc ttaccttcct ctataggcat
    13921 acgaaaaata ctcctccctc aataacaata gaactgctat ggatataata tattaaaacc
    13981 cttctgctgt caatattgtg cctgattctc cctgttctgt atatttctat cagccaaggt
    14041 gcgcagactt agaatagtgt cattctttct catcggagga ttggtctata tagacctttt
    14101 cccccggttt aaccaagccc aattgctccc tggccaactt ttctatatac tcaacagaag
    14161 ttgcattatt aatcatttcc atcagcctgt cattgtcatt tctgatagct tctattttag
    14221 agatttcatc ctgctctgac tttcttaggt cataaattgt aagctgctga ttgactattg
    14281 taatggcagc ataaactata aaagcaatca tgaatatatt cttaagccta agcttccgct
    14341 ttttcatata catcgcctca aacctttatt aatatagtat gtacaacttt ttcacatatt
    14401 actttaattt ctaagcaaaa tcaaacactt caactgattc agcaatgata aatcttcttc
    14461 acggcatatt cagcattatt aatattcgac ggtggtcagg aaaatccttt attactgttt
    14521 tcttttctct cttttctttt cactataagc ctatagtact tttttgagtc atatattact
    14581 tgtttcggca atccgcatac ttttcttaat ctgatttttt tcttccccaa tatacgtatc
    14641 aagctgcaac agcaactttt tacaaatgaa caggccatct ttaagggcag ggccaggata
    14701 ctgccggttt tcataaaaaa actgctgata ctcttgtata acaaatgaaa cagtcctgat
    14761 gctatcctcc caaggagctt ttcatacaaa tagaatccga ccagaaatcc aataagtaca
    14821 taacttctta tttctccgta gctgctggag aaaatcgccc atattacaac tgcgaatacc
    14881 gagaaaagaa acagtatgtc ccataaggag gtaatcagtc tttcagtttt ttttcttcct
    14941 cttataaccc tgtaaatatc atatagaatt cctatgatta ttccgccata taacgaatat
    15001 aaaaatacat aactctgaaa ttgaacggtt tccactgaat aacctcccta ttgctcaaaa
    15061 aatggaggag gcgcaataag aaaccagtaa cacgagagac acgaaaatac acgaaaaaca
    15121 cgaaggaaaa atattacgat acattgagaa ttatcaataa tatcgtaagt atcgaaaatc
    15181 gctatttcat cgatgaattg tataaatgtt accgtgtaat cgcgcagcga ttccgtgaaa
    15241 tatcgtgtgt ttagtgttac ccgttaccta tgtcgcaatt ttcttctgat actcccctat
    15301 ttaaaaatat tgcttaagaa actgccggtt tttttgatat cgattttgtc gttatatacg
    15361 caagaaatta tgtaaccttc gataaataga ttgccgtcat caacgttcag cctgttaatg
    15421 tgcaaatcag ttcctcttat aacaaggccc cccgcctccg tttccaatga aacttcattt
    15481 tcattaaaac tggctacatc tctgactccg ttgataataa gcctttctcg cccttcaatc
    15541 gttatactgt gaggttttga cttaatgagc ttcttttctt ccataaaaat ccccccaccg
    15601 atggtatatt gcttactagt atttatatgc atggagggtg tatttatgaa taagaagtgc
    15661 gcccttcggc gcacttctta tataagctta tacatctctc ttgctga
//
LOCUS       DAOOAB010000059        15045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_5138_length_15045_cov_16.427018, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000059 DAOOAB010000000
VERSION     DAOOAB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15045
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5138_length_15045_cov_16.427018"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="ytvI"
                     /locus_tag="PKU88_10810"
     CDS             complement(<1..879)
                     /gene="ytvI"
                     /locus_tag="PKU88_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HPX69800.1"
                     /translation="MLQPDLEKYLNIILRILVYIAMALIALFAFKFVFAYFMPFVIAL
                     ILSMLIEPLVLFLQKLKLKRGISVVLSILLFLGGFVAISTFAITRIVYELMKLYNRLP
                     KYYDGIYNFSAEVIQQVTDLYLQLPQEALNIMQEVLRTVFGKITSFLSHTTTWLIDTL
                     TVLPSTLIFILITLIATFFITKDKGLIKDFIFRQLPPTWSAKITSLKTDLLVALIGFL
                     KAQSIILSVTFIESFIGLTIIGIDYAFILAIIIAILDILPVLGTGGIYVPWGITNLVC
                     VYRNIRTAIPETSGHET"
     gene            1255..2724
                     /locus_tag="PKU88_10815"
     CDS             1255..2724
                     /locus_tag="PKU88_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX69801.1"
                     /translation="MNIDCIPILKSLRAYKNEGAVPFHMPGHKRGNIYKKTGLDFLSE
                     DILALDTTEVPGVDNLHCPEGAVLEAQGLAAEAFGADYTFFLVNGTTSGIYSMIMASA
                     NPGDKIIIPRNCHRSVYGAVILGRLIPVYIEPEIDYELGIAMGIKPETLESILEEHSD
                     AKAVVITNPTFYGICSDLKKIAEIVHNRGMLLLVDEAHGSHFCFNERLPISALEAGAD
                     MTSQSIHKTLPSMTQSSMLHVKSERVDIEKLKIFLQLVQTTSPSHILLASLDAARFIM
                     QQYGHELLDDAIDWSNWARSEINDTEGCCCLGPDRAGRYGAVDFDPTRLTVNFGASGL
                     SGTKAEEILRKNFKIQVEMSDLYNIVAITTIGDYRSDYETFVNAVKKLVETAEKRKAK
                     AMAPAVFFRPQEPSLMPWEAVYYEKEQVDMGQSIGRICGEMIIPYPPGIPVLMPGETI
                     SREIYEYLKLCVEYGIKLNGISDSKLKKVWVLKNNKKEV"
     gene            2728..3057
                     /locus_tag="PKU88_10820"
     CDS             2728..3057
                     /locus_tag="PKU88_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HPX69802.1"
                     /translation="MKLIIAVVQDDDVEDLVETLVKEKVYSTKLASTGGFLREGNTTL
                     LIGVEKDKVDSVISIIKDICKSRKQTFTTPIPPTGSAGVYIPYPIEVMVGGATIFVVD
                     IDRFEKV"
     gene            3070..3522
                     /locus_tag="PKU88_10825"
     CDS             3070..3522
                     /locus_tag="PKU88_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HPX69803.1"
                     /translation="MKITEIKTHPSVISTVAKEDKKSDIGSSKSSFENTLRKTEEQDL
                     ASRLNRLMSDIEKQGDNLVKSMDIKELKKYKKLIAEFMDEVVNNSLKFSKQSHFDRRG
                     RHKVYALVRKVNAKIEELSREFLKDEKDNIKILESIGNIKGMLLDMYI"
     gene            3551..4555
                     /gene="holB"
                     /locus_tag="PKU88_10830"
     CDS             3551..4555
                     /gene="holB"
                     /locus_tag="PKU88_10830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPX69804.1"
                     /translation="MGFNDIIGQRVIVESLRNAVKSNMISNGYVFSGPKGCGKKLMAF
                     IFAMALNCGGEANDKPCGRCSSCMRTSSGNHPNLKIVRPIGQSIKIKQVRGIISDSAR
                     KPFESGYKIIIIDNADKMTIDAQDAFLKTLEEPPENTVFLILTENHSLLLPTIVSRCQ
                     VYRFKPVDSLEMKAFIKSKYDYPAMDVEAAVRHSKGIVGSALELLRDKGNFKARALYI
                     DILEKAMTGDGNDAILLASEAIEDKESAESFLEFSLSWFRDIMVLRESRGAYDNLIIN
                     ADSLEVLTRHNSFLTEAKLNSIMEIINKSYRYIKHNVGIKNSIDGMLFNIAEVNSYNG
                     "
     gene            4548..5429
                     /locus_tag="PKU88_10835"
     CDS             4548..5429
                     /locus_tag="PKU88_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HPX69805.1"
                     /translation="MVRTVGVRFKKAGKIYYFDPGDLEINIDDNVVVETIRGIEFGKA
                     VLGIREVPEEEIIAPLKKVLRIATEEDNRIYENNKLKEQEAFNICLQKINHHNLGMKL
                     IDVEYTFDNNKIIFYFTADGRVDFRELVKDLASVFKTRIELRQIGVRDEAKMISGIGP
                     CGKSLCCSTFLGDFAPVSIKMAKEQNLSLNPTKISGICGRLMCCLKYEHETYECARRY
                     MPKIGALVDTPEGRGEVVESNILLEMVKVKLELMEGNLRVIKDFHVKDIVQLRPGKDI
                     EPDIEGDIENLELLEDN"
     gene            5519..5689
                     /locus_tag="PKU88_10840"
     CDS             5519..5689
                     /locus_tag="PKU88_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX69806.1"
                     /translation="MAYKIQDTCISCGACESECPVSCISQGDDKYVINPDECIECGAC
                     ANVCPVDAPVQD"
     gene            5745..6485
                     /locus_tag="PKU88_10845"
     CDS             5745..6485
                     /locus_tag="PKU88_10845"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HPX69807.1"
                     /translation="MEQLVHRDETVDDLQNGFSIIQKKHSFRYGVDAVLLADFADIKH
                     RHSVIDLGTGTGIIPILIYSRKKPVEITAVEIQKDMADMAARSIVLNGLEKNIKVLCM
                     DIKDAPMLLGKSRYDCVVTNPPYMKKECGINNPSETKAIARFEIMCSLEEILIAAREL
                     LKPGGKLFMVHRADRLADIIYEMRDKGIEPKRIRFVHPSIGKRPNLLLIEGARGGNKE
                     LKFMDPLYIHDENGEYTEEIHRIYGRKV"
     gene            6485..7393
                     /gene="rsmI"
                     /locus_tag="PKU88_10850"
     CDS             6485..7393
                     /gene="rsmI"
                     /locus_tag="PKU88_10850"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPX69808.1"
                     /translation="MTGILYLCATPIGNLEDITLRCVRVLREADIIAAEDTRQAIKLL
                     NHFQIRKPLISYHEHNKHEKGKEIVELLRSGKNIALVSDAGMPAISDPGEELVRLCID
                     SGIEIIPLPGPSASLTALIVSGLSTRRFCFEGFLPSNKKERKERLKKLSAETRTIIVY
                     EAPHRLLDTLGEIMEYLGDRRISVSREITKKFEETVRGEISSVIGIFKERAVKGEFVL
                     IIEGISEKELVDIELKKWDNVSVEEHIAMYLQQGADKKEAIKMVAEDRKLPKKEVYKI
                     GCNLERRTKNAIHKGYDSSSDIKSKS"
     gene            7341..7535
                     /locus_tag="PKU88_10855"
     CDS             7341..7535
                     /locus_tag="PKU88_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPX69809.1"
                     /translation="MQFTKDMTVAQILRANPKTAEVFLRYGMHCLSCPGATGESVEQA
                     AMVHGFDADQILDDLNKVSE"
     gene            complement(7604..7843)
                     /locus_tag="PKU88_10860"
     CDS             complement(7604..7843)
                     /locus_tag="PKU88_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPX69810.1"
                     /translation="MKSTGIVRKVDELGRVVIPIELRRTLNIEEKDSLEIYVDGEHII
                     LKKYEPACIFCGNAKDVVQYKGKNICPACMEEFKK"
     gene            8090..9658
                     /locus_tag="PKU88_10865"
     CDS             8090..9658
                     /locus_tag="PKU88_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX69811.1"
                     /translation="MGKNTDTYHIDSDIPPESFWMASTARTNYPALGNDIKVDVAIVG
                     GGMAGITSGYMLKKEGLSVAVIEADRIIQGTTGHTTAKITSQHDLIYSKIKNYMGEEK
                     ARQYAVANETAIRVISDLIEQEKIDCDFHKESAYVVTLTDSYVQQIADEANTAGSLGI
                     KSSFIENTPLPFKVKGAVKFDGQARFHPRKYLLALAKKVDGGGSFIFEQTRVVDINKG
                     NPCILVTNLGKKVTADNVIIASHFPCFDGMGFYFARMYPDRSYAIAVRAKEKFLGGMY
                     ITAEDPGRSLRSQEYEGGELIIVSGEHHKTAHGTNLHRHYENLLEFAKKHYDVQEQLF
                     RWSTQDYTTLDKVPYVGRLTSKIQNIYVATGFRKWGMTNGTASAMILKDLILNGRNPW
                     AEVYDPSRFVLNPSITSFISINADVAAKLASGKLKPVPEDIEVEKGEAAIADIEGNKM
                     GIYRDKKGQLHRVDTTCTHLGCELKWNDAERSWDCPCHGSRFTYEGEIIEGPAISELQ
                     HDSNEKNKIDPNIV"
     gene            9936..10700
                     /locus_tag="PKU88_10870"
     CDS             9936..10700
                     /locus_tag="PKU88_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPX69812.1"
                     /translation="MGELTLGLVQMRCEKGAIEYNIISMYKYLEECRNKGVDIVCFPE
                     MNISGCINPLKYSNALLSMFHPSIQRIADMSFLYQTIIIAGFAEKNYGGKPYITQFVA
                     KDGKIEGCYRKRTLRGDQSKWFSPGTKPFMFRYSGYNFGLTISQDIYDGGIFKEYAER
                     GASLVFESAAPGLSVGQGVGDWEPGFSSWKNECKSKLGKYAAEDGIFIAAATQAGRTC
                     DEDFPGGGYVFAPDGRCMYEMEDGEEGMLVAKIQLN"
     gene            10767..11354
                     /locus_tag="PKU88_10875"
     CDS             10767..11354
                     /locus_tag="PKU88_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPX69813.1"
                     /translation="MINKLWFYMIAIAVAFAATGRRLEILTEEIFSSLGAAVELGIGL
                     LGGMMLWCGVLKVAEKSGLADKISDFIRPLMKLLFRGIPPKSAAMGAMIMNITSNMLG
                     LGNAATPMGLKAMQELQQLNPRKDTATNAMCLFLVVNAAPIQILPATVLSLRASVGSA
                     NPGIIILPAVASSFAAVSAGVITCKLLEKTCGGRA"
     gene            11351..11860
                     /locus_tag="PKU88_10880"
     CDS             11351..11860
                     /locus_tag="PKU88_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPX69814.1"
                     /translation="MNFIIPIIILLILIYGYLNGVKVYDAFIEGAGEGLLITFRILPY
                     IGAMLLAVGMLRNSGGLDFMLYVLSPLTSAAGIPEEVVPLIIMKPLSGSGALGILADI
                     LKRSGADNYAGVLASIIMSSTETIFYTITVYFGSIGIKNIRHTLAAAVIADMAGLAAA
                     VLLCRFILF"
     misc_binding    11926..12174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12212..14116
                     /gene="metG"
                     /locus_tag="PKU88_10885"
     CDS             12212..14116
                     /gene="metG"
                     /locus_tag="PKU88_10885"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPX69815.1"
                     /translation="MTDKKTYYITTPIYYPSDKLHIGHTYTTVAADAAARFKRMLGYD
                     VMFLTGTDEHGQKIQRKAEEKGATPKQYVDEVVAGIKDLWKLMQISNDRFIRTTDRQH
                     EEAVQKIFKKLYDQGDIYKSEYEGWYCTPCESFWTERQLVDGKCPDCGREVELTKEES
                     YFFKLSKYQDRLIKYIEENPDFIQPQTRQNEMLNNFLKPGLDDLCVSRTSFNWGIPVS
                     FDEKHVVYVWIDALSNYITALGFLSDDDSDYKRYWPADVHLVGKEIVRFHTIIWPIML
                     MALGEPVPRQVFGHGWLILEGGKMSKSKGNVVDPVVLVEKYGVDAIRYFLLREVPFGS
                     DGVYSEEALVNRMNSDLANDLGNLLSRTITMIDKYFEGVIPAAKNSGEPLDADLIKLA
                     ESTPIKVEELMDKLLFSNALTEIWKLIGRSNKYIDETMPWVLARDESKKERLAEVLYN
                     LAEVLRIVGVLISPYMPNTPAKIFQQLGIEDEALKTWESIKSFGALKAGTIVNKGEII
                     FPRVEFGGENAAPETPKEDNYITIDDFAKIDLRVAKVLECEKVEGSDKLLKLQLEVGS
                     EKRQVVSGISKYYAPEDLIGKSVVLVANLKPVKIRGVESNGMILAADAGDSVVVVSPL
                     SEIPSGSKVR"
     gene            14233..15003
                     /locus_tag="PKU88_10890"
     CDS             14233..15003
                     /locus_tag="PKU88_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPX69816.1"
                     /translation="MLFDTHAHLDDGRFDKDRDEAISQCKQDGVELILNAGSNIETSV
                     KAIALSKKYEFIYASVGVHPHDAASMDEETAGVLAALAGNRKVRAIGEIGLDYHYDLS
                     PRDVQKQRFIEQIDIARQLKLPIIVHDREAHGDVMDIFKKTRIKEVGGVLHSFSGSAE
                     MALECIKLGLYISISGPVTFENARKTVEAVKQVPLDMLLIETDSPYLTPVPHRGKRNY
                     PGYVRFVAEKIAEIKGISFEEVARQTLENGKRLFGIEE"
BASE COUNT         4761 a         2580 c         3762 g         3942 t
ORIGIN      
        1 ggtttcgtgt ccggatgttt ccggaatcgc cgtccggatg ttccggtata cgcatacaag
       61 attggttatc ccccaaggaa cataaatgcc tccggtgcca agaacgggca aaatatctaa
      121 aatagcaata ataattgcca ggataaatgc gtagtcaata cctataatag tcaaccctat
      181 aaaggattca ataaatgtta cacttaatat gatggattga gctttcaaaa agcctatcaa
      241 ggctactaaa agatctgttt taagtgaagt tattttagca ctccatgttg gcggaagctg
      301 cctgaaaata aagtccttaa tcaagccttt gtcctttgta ataaagaatg ttgcaataag
      361 agtaatcaat atgaatatca aggttgacgg aagtacggtt aaggtatcta ttagccatgt
      421 agttgtatgg cttaagaaag acgttatttt tccaaagaca gtcctcagaa cttcctgcat
      481 gatattgagg gcttcctgag gcagctgcag atataagtcg gttacctgct gaattacttc
      541 agctgaaaag ttatatatcc cgtcataata ttttggcaga cggttatata gtttcattag
      601 ctcatacact atccttgtaa ttgcgaaggt tgaaatagcg acaaatcctc caaggaaaag
      661 caaaatagac aatactactg agatgccccg tttaagctta agcttttgaa ggaataatac
      721 cagcggttca attaacatgg aaagtatcaa ggcaataaca aagggcataa aatatgcaaa
      781 aacaaacttg aatgcaaata atgcaatcaa ggccatggca atgtaaacca aaattcttaa
      841 tattatatta agatattttt ctagatccgg ctgaagcata acatcacttc caaaaatatt
      901 taaatctgct gaaagaatcc catactacta aatatgctga taaaaagaat atgcaactat
      961 atatttatat tatattaaat gaaagtgtaa taatcaaaca atatcgaata tgcagagctg
     1021 ttttttggag cataatgcca atttggcaat tattttggag tgtcttgtag aaactttcat
     1081 atcatcatat tataaacaac caagagcata tatagtataa ttaaagaaat aaaattattt
     1141 cagcttaata attgccaaga accagataat tatacaagag acaaaaaagt ttctcagtta
     1201 gaataggaaa taccggttaa ataattaaaa ttactgatac ggaagggata tctgatgaat
     1261 attgattgca tacccatatt gaaatcgctt agagcatata agaatgaagg ggctgtgcct
     1321 tttcatatgc cgggacataa aaggggaaat atatacaaga aaacaggcct tgattttctg
     1381 tctgaggata tattagcctt ggatacgact gaagtgccag gagttgacaa tctgcactgc
     1441 ccggagggtg cggttctcga ggcgcaaggc ctcgccgcgg aagcatttgg agcggattat
     1501 acatttttcc ttgttaacgg caccacctcg ggtatatatt caatgataat ggcatccgca
     1561 aatcccggag acaaaataat tattccccgt aactgccata gatcggtata tggtgcagta
     1621 atacttggga ggctgatacc ggtatacatt gagccggaaa tagactatga gcttggaata
     1681 gcaatgggga taaagcctga aacattggaa agtatactag aagagcacag tgatgcaaaa
     1741 gcggttgtaa tcactaatcc cactttttat gggatatgtt ctgatttgaa gaagattgca
     1801 gaaatcgtac ataatagggg aatgctgctg cttgtggatg aggctcacgg ttcacatttc
     1861 tgcttcaacg aaaggcttcc catatctgca cttgaggcgg gagcggacat gacatctcaa
     1921 agcatacata agaccctgcc ttctatgact caaagttcaa tgctccatgt gaaatctgaa
     1981 agggtagata ttgagaagct gaagatattc ttgcaattgg ttcagacgac gagcccttcc
     2041 catatactgc tggcatcttt ggatgcggca aggtttatta tgcagcaata cggacatgag
     2101 cttttggatg atgcaataga ttggagcaat tgggcgagaa gcgaaatcaa tgatactgaa
     2161 ggatgctgct gtttaggccc ggacagggca ggccggtatg gagcggttga ttttgatccg
     2221 acaaggctga cggtaaattt tggagcgtca ggcctaagcg gtaccaaagc tgaggaaata
     2281 ttgagaaaga atttcaagat acaggtggaa atgtccgatt tatataatat agttgcaatc
     2341 acaacgatag gagattacag gtctgattat gaaacttttg taaatgcagt gaaaaaactt
     2401 gtggagactg cggagaaaag aaaggcaaaa gctatggcgc cggcagtatt tttcaggccg
     2461 caggagcctt cccttatgcc gtgggaagcg gtatactatg aaaaagagca ggtggatatg
     2521 gggcaaagca taggaagaat ctgcggagaa atgataatac cgtatccccc gggaatacct
     2581 gttttgatgc cgggagaaac aatcagcaga gagatttatg agtatctgaa gctttgtgtt
     2641 gaatatggaa taaagctgaa cgggatttct gattccaagc tgaaaaaggt atgggtgtta
     2701 aaaaataata aaaaggaggt atgagggatg aaacttataa tagcggttgt acaggatgat
     2761 gatgtggagg atctggtaga aacgctggtc aaggaaaagg tctatagtac aaagcttgct
     2821 tctacgggcg gatttctcag ggaagggaat acaacacttt tgataggtgt tgagaaggac
     2881 aaagtggatt cggtaatatc aataattaag gatatatgca aaagcagaaa gcagaccttt
     2941 actacgccaa ttccgcctac aggctctgca ggggtttata ttccatatcc cattgaagtg
     3001 atggtcggcg gtgcaactat ttttgtagtt gacatcgaca ggtttgagaa ggtctaaggg
     3061 tgaagtatta tgaagataac agaaatcaag acccatcctt ctgtaatcag cacagttgcg
     3121 aaagaagaca aaaaatcaga tatcggcagt tccaaatcaa gctttgagaa tactctcaga
     3181 aagacagaag agcaggattt ggccagccgc ctgaacaggc ttatgagcga tattgagaag
     3241 caaggggata accttgtcaa aagcatggat ataaaagaat taaagaaata taagaagctt
     3301 attgcagagt ttatggatga ggtagtgaat aattccctta agttttcaaa acaaagccat
     3361 tttgacagac ggggaaggca taaggtatat gcccttgtga gaaaggtcaa tgccaagatt
     3421 gaggagttaa gcagggaatt tctcaaggat gagaaggata acataaagat acttgaaagc
     3481 attggaaata ttaaaggtat gttgttggat atgtatatat agaattttta ataccgaggg
     3541 agaagaaacg atggggttta atgacataat tgggcagagg gtaatagttg aaagcctgag
     3601 aaatgcagtg aaaagcaata tgatttcaaa cggctatgtt tttagcggac ctaagggctg
     3661 tggcaagaag ctaatggcct ttatttttgc aatggctttg aactgcggcg gtgaagccaa
     3721 tgacaagcct tgcgggaggt gcagctcttg tatgagaaca agcagcggca atcaccccaa
     3781 tttgaagatt gttaggccta tcgggcagag cattaaaata aaacaagtcc gcggcataat
     3841 aagtgattca gcaagaaagc cctttgaaag cggatataag ataataataa tagataatgc
     3901 ggataagatg actattgatg cacaggatgc atttttaaaa actcttgagg aacctcctga
     3961 aaacactgta tttttgatac ttacggaaaa tcatagcctc ttattgccta caattgtctc
     4021 cagatgccag gtatatcggt tcaaacctgt ggacagcttg gaaatgaagg cttttataaa
     4081 atccaaatat gattatccgg caatggatgt tgaagcagct gtaaggcatt caaagggaat
     4141 agtcggaagc gcgctggagc ttctgaggga taaaggaaac ttcaaggctc gtgcattata
     4201 tattgatatt ctggagaagg caatgacagg ggatgggaat gatgcaattc ttctggcatc
     4261 agaagcaatt gaggataagg aatctgcaga gagctttctt gagttttccc tgtcctggtt
     4321 cagggatata atggtattga gagaaagccg gggggcttac gataatctta ttatcaacgc
     4381 tgacagcttg gaggttttga cgaggcacaa ttcttttttg acggaagcca agttaaacag
     4441 tataatggag ataattaata agagttatag atatatcaag cacaatgttg gtataaaaaa
     4501 cagcattgat ggtatgctat ttaatattgc ggaggtaaat tcatataatg gttagaacag
     4561 ttggagtaag gtttaagaaa gcagggaaga tatattattt tgatcccggt gatttggaaa
     4621 taaacattga cgataatgtt gtggtggaga ccataagggg tatagagttc gggaaagccg
     4681 tattgggaat aagagaggta cctgaggaag aaataattgc gcctcttaaa aaggtgctga
     4741 gaattgcaac agaggaagac aacaggatct acgaaaataa taagctgaaa gaacaggaag
     4801 cctttaacat ttgcctgcag aagattaacc atcataatct tggtatgaag cttattgatg
     4861 tagaatatac ctttgacaac aacaaaatca tattctattt cactgcagac ggacgtgtgg
     4921 atttcagaga gcttgtaaag gacttggctt cagtttttaa gacaaggata gagcttcgtc
     4981 agataggtgt cagagatgaa gccaagatga taagcgggat aggcccttgc ggcaaaagtc
     5041 tatgctgttc aacgttcctt ggagattttg cgcctgtgtc cataaaaatg gcaaaggagc
     5101 aaaacctgtc ccttaatccc acaaagatat cgggtatttg cggaaggctc atgtgctgtc
     5161 ttaaatatga gcatgagaca tatgagtgcg ccagaagata tatgccaaaa ataggggcat
     5221 tggtggatac tcccgaaggc cggggtgaag tggtggaatc caatatactt cttgagatgg
     5281 tcaaggtaaa gcttgaatta atggaaggga accttcgtgt tatcaaggat tttcatgtaa
     5341 aagatatagt tcagttaagg ccggggaagg atatcgaacc tgatatagag ggagacatag
     5401 aaaacctgga actgcttgaa gacaattagt gaaaaattaa acaaaaacag tacaaggggt
     5461 tttatttata atagaatagt gatagaatgt atttgtaatt tatttaggag gtgctactat
     5521 ggcatataaa attcaggata cttgcatcag ctgcggtgca tgtgagtcgg aatgtccagt
     5581 aagttgtata agtcagggcg atgataaata tgtaataaat ccggatgagt gtatcgaatg
     5641 cggtgcgtgt gccaatgtat gcccggttga tgctccggtg caggattaag agttactaga
     5701 aaaaagaccg aaaaggtctt tttttcgatt tagcgaggtg tgtcatggaa cagttagtgc
     5761 atagagatga gactgtcgat gatttacaga acggatttag tataattcag aaaaaacact
     5821 ctttcagata tggcgttgat gctgtgcttc ttgcggactt tgccgatata aagcataggc
     5881 attcggttat agacttgggc accggaaccg gtataatccc cattcttata tattccagga
     5941 aaaagcctgt tgagattact gcggtggaaa tacagaagga tatggctgac atggctgcgc
     6001 gcagcatcgt attaaacgga ctggaaaaaa acatcaaggt gctgtgcatg gatattaagg
     6061 atgcaccaat gcttttgggc aagtcaaggt atgattgcgt agtgaccaac cctccctata
     6121 tgaaaaagga atgcggtata aataaccctt ctgaaacaaa agcaatcgca aggtttgaaa
     6181 taatgtgcag cctggaggag atattgattg cagcaaggga attgctgaag cccgggggga
     6241 aacttttcat ggtccatagg gccgaccgcc tggcagatat aatttatgag atgagagata
     6301 aaggcattga accaaaacgc ataagatttg tacacccgtc cattggcaaa aggcccaatc
     6361 tgttattgat tgaaggagca agaggaggga acaaggagct taagttcatg gatccccttt
     6421 atatacatga tgaaaatgga gaatatactg aagagataca cagaatatac gggaggaaag
     6481 tatgattacc ggtattttat atctatgcgc aactcccata ggaaaccttg aagatattac
     6541 cctgaggtgc gtcagggtat taagggaagc ggatatcata gccgcagagg atacccgtca
     6601 ggcaataaag ctgctgaatc attttcaaat ccgaaagccc ctgataagtt atcatgaaca
     6661 caataagcat gaaaaaggca aagagattgt tgagctgtta agatcaggga agaatattgc
     6721 tttggtttct gatgcgggta tgcctgcgat ttcagatccc ggagaggagc ttgtaaggct
     6781 ttgtattgac agcggtattg aaattattcc gctgccgggg ccttctgctt cacttacggc
     6841 attgattgtt tcggggcttt caacaagaag attctgtttt gaaggctttc ttccgtcaaa
     6901 taagaaggag aggaaggaaa ggctcaaaaa gctctccgcc gaaacaagaa ctataatagt
     6961 gtatgaagcg cctcatcgtt tattggatac tttgggcgaa atcatggaat accttggtga
     7021 caggaggata tccgtttcaa gagagataac aaagaaattt gaagaaaccg taagaggaga
     7081 aataagcagc gtaatcggta ttttcaaaga gcgggctgta aaaggtgaat tcgtactgat
     7141 tattgaaggc ataagtgaaa aagaacttgt tgatattgag ctgaagaaat gggataatgt
     7201 atctgttgag gagcatattg caatgtattt gcagcaggga gcagacaaaa aagaagcaat
     7261 taaaatggtt gcagaggata ggaagctccc gaaaaaggaa gtatataaaa taggatgtaa
     7321 ccttgaaagg aggacaaaaa atgcaattca caaaggatat gacagtagct cagatattaa
     7381 gagcaaatcc taaaactgct gaagtttttt tgaggtatgg tatgcactgc cttagctgcc
     7441 cgggtgccac aggagaatct gtggaacagg ctgcaatggt gcatgggttt gatgcagacc
     7501 agattttgga tgacttgaac aaggtaagtg aataaaataa ggggggacgt ttctcttgcc
     7561 ccgcaagcgg gacaagagaa acgtcccccc ttattcgata atactatttt ttaaattctt
     7621 ccatgcaagc cggacaaatg ttcttaccct tgtattgaac tacgtctttt gcgtttccgc
     7681 agaatatgca tgccggctca tatttcttca gtatgatatg ctcgccgtca acgtatattt
     7741 ctaaggagtc cttctcctcg atattaaggg tcctcctcaa ttcaatcggg attacaactc
     7801 tgcctaattc gtcgactttt cttacaattc ctgtagattt catgtactct cctcctttta
     7861 cattatacaa caaatttcga caatttctac tatgattaaa tgttaacatt tatgacaata
     7921 aaagtcaata gtgtataaaa ataaattaat aattattatt tattatctcc aacatagctt
     7981 aagcccatgc gctgcaagcc ttggggattt atcggaatat ttttcgcggg tatgtaaatg
     8041 atatatattg gtttaaaatg ttacaaatct ataaggagtg aaaggattaa tgggaaaaaa
     8101 taccgataca tatcatatcg attccgatat acctcctgag tcattttgga tggcatcgac
     8161 tgccaggacc aattatccgg ctttgggaaa tgatatcaaa gtggatgttg caatagtagg
     8221 gggcggtatg gcaggaataa catcagggta tatgctgaaa aaagaaggct taagtgtagc
     8281 tgtaatcgaa gctgacagaa ttattcaggg cacgacaggg catacaacgg caaaaattac
     8341 ttcccagcac gatctgatat acagcaagat aaagaattat atgggagagg aaaaagcaag
     8401 gcaatatgct gttgccaatg aaacagcaat ccgcgttatt tcagatttga tagaacaaga
     8461 gaaaatagac tgtgattttc ataaggagtc ggcttatgtc gttacattga cggattcata
     8521 tgtccagcag atagcagatg aagcaaatac ggcaggcagt ttgggaataa aatcatcgtt
     8581 tatagaaaat accccccttc ctttcaaggt taaaggagct gtgaagtttg acggccaggc
     8641 gcggtttcat ccgcgcaagt atctgctggc tctggcaaag aaggtagacg gaggcggcag
     8701 ttttatattt gaacaaacaa gggttgtaga tattaataaa gggaacccct gcatccttgt
     8761 taccaacctg gggaaaaagg taacagccga taatgttatt attgcttctc attttccgtg
     8821 ctttgacggc atgggctttt atttcgccag gatgtatccg gatagatcct atgctattgc
     8881 ggtaagggcg aaggaaaaat tcctcggagg tatgtatatt actgcggaag accccggcag
     8941 gtcactgcgc tcccaggagt atgaaggcgg tgagcttatt attgtctcgg gggagcatca
     9001 caagacagct catggaacga atcttcacag gcattatgaa aacctgttgg aatttgcgaa
     9061 gaagcactat gatgtgcagg aacagctttt cagatggtca actcaggatt acaccactct
     9121 tgacaaagta ccctatgtgg gaaggctgac ctccaagatt caaaatatat atgtggcaac
     9181 gggcttcaga aagtggggaa tgacaaacgg cacagcttcg gcaatgatat tgaaggattt
     9241 gattcttaat ggaagaaatc cgtgggctga ggtttatgac ccttcaaggt ttgttttgaa
     9301 tccttcaata acaagcttta tatcaataaa tgcggatgtt gcggccaagt tagcctcagg
     9361 aaaactgaag cctgtgccgg aggatataga ggttgaaaag ggagaggcgg caattgcgga
     9421 tattgaagga aacaaaatgg ggatttaccg tgataagaag ggacagcttc atagggtaga
     9481 tactacctgt actcatttgg gctgcgaatt gaagtggaat gatgcggaaa ggtcctggga
     9541 ctgcccgtgc catggttcca gattcacata tgagggagaa ataattgaag gccctgcaat
     9601 tagtgaattg cagcatgaca gcaatgagaa gaacaagatt gatcctaata ttgtataatt
     9661 tctcattacc cttgtttccc tcctaaagca aaaagtatga ccccttttta aaaaaataaa
     9721 ggaaatttgt aacaggtgta gaagtatatt acatgcagtt tttattttat aggaaagtat
     9781 actttcctat aaaatggggg atgcaagggg gagaagcccc ctgccgttca aggagggtgc
     9841 tcaggatgcg gagttgacgc cttcgggaac catagggttc cctagggtca aaaacgcgtc
     9901 gtgagacgca ttttttgctg agggaggttt ggacaatggg agaactgacc ttgggacttg
     9961 ttcagatgag atgcgagaaa ggggcaatag agtataacat aataagcatg tataaatatc
    10021 ttgaggaatg cagaaataaa ggcgtggata tagtatgttt tcctgaaatg aatatcagcg
    10081 gctgtatcaa cccgctgaaa tactctaatg ctctgctttc catgtttcat ccctcaattc
    10141 agcgtatagc cgatatgagc ttcttgtacc agacaatcat tatagcaggt tttgcagaaa
    10201 agaactacgg gggaaagccc tacataactc agtttgttgc aaaagacggc aagatagaag
    10261 gctgctacag aaaaagaact ctgcggggtg atcagtcgaa atggtttagc ccgggtacga
    10321 agcccttcat gtttaggtat tcgggatata attttggact gacaataagc caggatattt
    10381 acgacggcgg aatttttaag gagtatgccg agcggggtgc cagtttggtt tttgaaagcg
    10441 cagcacccgg attatcagtc ggacaaggtg tcggagattg ggagcccggg ttcagtagtt
    10501 ggaagaatga atgcaagagc aagctgggca aatatgcagc agaggatgga attttcatag
    10561 cagcagcaac ccaggcagga cgtacatgcg atgaggattt cccgggaggt ggctatgtat
    10621 ttgctcccga cggcaggtgc atgtatgaaa tggaggatgg ggaggaaggc atgcttgtcg
    10681 ccaaaataca gttaaattaa accacataaa acaatcatat ccgccataca ttttattatt
    10741 aataaatgta tggcggtgtt tttttaatga ttaataagct ctggttctat atgatagcca
    10801 tagctgtcgc cttcgcagcg acaggaagaa ggcttgaaat cctcactgaa gagatatttt
    10861 cctctctcgg tgctgctgtt gaacttggaa ttgggcttct gggaggcatg atgctttggt
    10921 gcggggtttt gaaggtggct gaaaaatcag gattggcgga taagatatcc gattttatca
    10981 ggcctcttat gaagctgctt ttcaggggga tacctcccaa aagtgctgca atgggagcta
    11041 tgataatgaa cataacctcc aacatgcttg gacttgggaa tgcggctact cccatgggac
    11101 ttaaggcgat gcaggagctg cagcagctga accccagaaa ggataccgca actaatgcaa
    11161 tgtgtctttt tctggtagtg aacgcagcgc ctatacagat attgccggca acggttctat
    11221 cccttagagc gtccgtaggt tcggcaaatc cgggaattat tatacttccg gcggttgctt
    11281 cgtcctttgc agctgtttct gcaggggtga taacctgcaa actgcttgag aaaacatgtg
    11341 gaggcagggc atgaacttta ttataccgat tatcatactg cttatactta tctacggata
    11401 tctcaacgga gttaaggtat atgatgcatt cattgagggg gcgggggaag ggcttctgat
    11461 aaccttcagg atactgcctt atataggggc aatgcttctt gcggtgggaa tgctcaggaa
    11521 ttccggcgga ttggatttca tgctttatgt tttgagcccg ttgacctcgg cagccggaat
    11581 acctgaagaa gtagtgccgc ttattattat gaagcccctg tcgggaagcg gcgcattggg
    11641 aatccttgcg gatatattga aaagaagcgg cgcggacaac tatgcgggag tactggcaag
    11701 tataataatg agcagtacgg agacgatttt ttatacaatt accgtatatt ttggttcaat
    11761 aggaataaaa aatatacgtc acacactggc agcagctgtg attgcagata tggcaggatt
    11821 ggcagcggcg gtactcctgt gcagattcat actgttttag cctccaatat tatatattga
    11881 cactccccaa atattgttgt atatttttat catataatta aaactataaa agtaatgatg
    11941 ggaagagtaa gttggcgtga ttgttaaaga gagtcgggaa aggtgcgagc cggtacataa
    12001 aaccaaccca acatggcccc tgaactgcac aggtgaggta tttgcttagc ttgtgccggt
    12061 aagctgacgt tatacagcac agtgatgaat cttaaggagg agtcacttgt cgagttttct
    12121 gccgtgaggc agggaagaat cagggtggta acacgagcag ctctcgtccc tttatgggat
    12181 ggagcttttt tattttaaag gaggaatttc tatgacagac aaaaagacgt attatatcac
    12241 tacgccgata tattatccca gtgataagct gcatatcggc catacttata ctactgttgc
    12301 agctgacgct gcagccaggt tcaagaggat gctgggctat gatgttatgt ttcttaccgg
    12361 aactgatgag catgggcaga aaatccagag aaaagcggag gaaaagggtg caactcccaa
    12421 gcaatatgtt gatgaagtgg tagccggcat aaaggacctt tggaaactga tgcagataag
    12481 caatgacaga tttataagaa ctaccgacag gcagcatgag gaagcagtgc agaagatatt
    12541 caaaaaactc tatgaccagg gagatattta taaaagtgaa tatgaaggct ggtattgtac
    12601 tccctgcgaa tctttctgga cagaaaggca gttggttgac ggcaaatgcc ccgactgcgg
    12661 aagagaagtc gagctgacaa aggaggaaag ctatttcttc aagctttcca agtatcagga
    12721 cagactcata aagtatattg aagaaaaccc tgattttata cagcctcaaa ccagacagaa
    12781 cgaaatgctg aataactttt taaagccggg attggatgac ctttgcgtct ccagaacctc
    12841 ctttaactgg ggaataccgg tttcctttga tgaaaaacat gttgtatacg tgtggatcga
    12901 tgccttgtca aactatatta cggcattggg attcttgagc gatgatgatt cggactacaa
    12961 gagatattgg cctgcggatg ttcaccttgt aggaaaagaa atagtcaggt tccatacgat
    13021 tatttggcca atcatgctca tggctctggg agaacctgtt cccaggcagg tgttcggcca
    13081 tggctggctg atacttgaag gagggaaaat gtcaaaatca aaggggaatg ttgtggatcc
    13141 ggtggtactt gtagaaaaat acggagttga tgcaataaga tacttcctgc ttagagaagt
    13201 tccctttggt tctgacggag tatattctga ggaagccctt gtgaacagga tgaattctga
    13261 tctggctaat gatctgggga atctgctttc cagaactatt acaatgatag ataaatattt
    13321 tgaaggagtc attccggctg ctaagaattc cggagaaccg ctggatgcag acctcatcaa
    13381 actggctgaa agtactccta taaaggttga ggagttaatg gataagctgc ttttctccaa
    13441 cgcgttaact gagatatgga aactgatcgg gagatccaac aaatatattg atgagacaat
    13501 gccttgggtg cttgccagag atgaatcaaa gaaagaaagg cttgcagaag ttctgtacaa
    13561 tcttgcagaa gtattaagaa tagttggagt tctgataagc ccctatatgc ccaatacccc
    13621 tgcaaagata ttccaacagc ttggcataga ggatgaagcc ttgaagactt gggaaagcat
    13681 aaaaagcttt ggggcattaa aggcaggaac gattgtaaat aaaggcgaga taatatttcc
    13741 aagggttgag tttggagggg aaaacgctgc tccggaaaca ccgaaggaag ataattatat
    13801 tacaatagat gatttcgcaa agattgactt gagggttgca aaggtattgg agtgcgagaa
    13861 ggttgaaggc tccgacaagc tgctgaagct gcagcttgaa gtgggaagcg aaaaacgtca
    13921 ggtagtgtcc ggcatatcca agtattatgc accggaagac ctgattggca agagcgtagt
    13981 gctggtggca aatctcaaac ctgtcaagat aaggggagtg gaatccaacg gaatgatact
    14041 ggcagcggat gccggtgatt ctgttgtggt agtttctccg ctttcagaaa taccgtcagg
    14101 ttccaaagta cgataattta gcaggtacgg ggttacgagg tacggggtac gagaaaagtc
    14161 cgagtattcc ctttacccct aaccctgttt cctttagtgt attattaaga actaaaagga
    14221 agtgagacgt aaatgctttt tgacacgcat gctcatctgg acgatggcag gtttgacaag
    14281 gacagggatg aagcaataag ccagtgtaaa caggatggag ttgaactgat tcttaatgcg
    14341 ggaagcaata ttgagacttc ggttaaggca atcgcattgt cgaaaaaata tgagttcata
    14401 tatgcttcag tgggggttca tccccacgat gccgcgagca tggatgaaga gacggcagga
    14461 gtgcttgctg cccttgccgg gaacaggaaa gtaagggcga taggagaaat cggactggac
    14521 tatcactatg atttgtctcc cagggatgtg cagaagcaga ggtttattga gcagattgat
    14581 attgcaaggc agctgaagct cccgattatt gtacatgaca gggaagccca cggggacgta
    14641 atggatatat tcaaaaaaac ccgtattaaa gaggtcgggg gagttctcca cagcttttcc
    14701 ggcagcgcgg aaatggcatt ggagtgtatt aagctggggc tgtatatatc cataagcggc
    14761 ccggtgactt ttgaaaacgc aaggaaaaca gttgaggcgg tgaaacaggt tcctcttgat
    14821 atgctgctga tcgaaaccga cagtccttat ctgacccctg tgccccacag gggcaaaagg
    14881 aattaccccg gctatgtcag gtttgtagcg gagaagatag cggaaataaa aggcatatcc
    14941 tttgaggaag tagccaggca gaccttggaa aacggaaaac gcctcttcgg aatagaagaa
    15001 taatgcgacg taggtaacgg gtaacacggg agacacggct gtttc
//
LOCUS       DAOOAB010000060        14441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_5392_length_14441_cov_16.743362, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000060 DAOOAB010000000
VERSION     DAOOAB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5392_length_14441_cov_16.743362"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKU88_10895"
     rRNA            complement(<1..284)
                     /locus_tag="PKU88_10895"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(859..1509)
                     /locus_tag="PKU88_10900"
     CDS             complement(859..1509)
                     /locus_tag="PKU88_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPX69817.1"
                     /translation="MKMKVRDPFSGFSHLAGAILSVAGLCLLIRYASMNGTVWHIVSF
                     SIFGASLILLYTASSIYHLLSVSEKSTRVLRKIDHMMIYILIAGTYTPVCLIPLRGGW
                     GWSLLISIWGIAMAGIVLKVLWFNAPRWLYTLFYLLMGWLIVIAFVPLVRTMPIAAML
                     WLIAGGLLYTVGAVIYGTKWPKLKSKVFGFHEMFHIFVLYGSFCHFWMMFRYILYL"
     gene            1697..2290
                     /locus_tag="PKU88_10905"
     CDS             1697..2290
                     /locus_tag="PKU88_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HPX69818.1"
                     /translation="MDFNEDSFFKIVEDISQGRDIRIPDIPDIELYMDQVTTFIDNKL
                     GYNKRILKDKILTKTMINNYTKSKILLPSKNKRYNRQHVILLILIYYLKQILSINDIS
                     ILFVPLFKNITSSNDGAGYLENLYNAFLEVKDNNANDLKSILSQKLDLISTKAEEFPN
                     DSKETSKILLTVLMLVATAGIQKRIAEKIIDDYLTKK"
     gene            complement(2314..3108)
                     /locus_tag="PKU88_10910"
     CDS             complement(2314..3108)
                     /locus_tag="PKU88_10910"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPX69819.1"
                     /translation="MKWLDKLERRLGRFAIKNLMSYIVFMTGIVYALSYFDRSGFVIS
                     KLMLIPELVLRGEVWRLVTYIFIPPNTSIIWILFTLYFYYMVGSALEQEWGSFKFNIF
                     YFTGMIGTTIAAFITGYGATALYLNLSLFLAFAKIYPDFELLLFFVIPVKMKYLAMLN
                     WAFIIYTVLVGDLSAKLIAVVSIMNYFLFFGKDIIAGTKSSRQVYNNRRRFQSELPRD
                     FTIHKCTICGKTEKDDPKMDFRYCPQCEGDYEYCMDHVRNHEHVRE"
     gene            complement(3141..3965)
                     /locus_tag="PKU88_10915"
     CDS             complement(3141..3965)
                     /locus_tag="PKU88_10915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX69820.1"
                     /translation="MIRPLNEWDRAIILDYLSRNEDEAAFVFGNILHCGITNRKDVLR
                     CGDYYGYFEGDNLRGILVFYNLGTCIPHYESVGAVPFLSKLMIMGRFNVLLGKDQIVR
                     PLFEAIKNYKELKRFEECSYQINRNFKPFKLEGAVFTDVSGNDDTSIISFVRKAYWRG
                     FGAERTIEGTRILLLQKNEEEEFIILSVDNKLVAQACIQTFTDNTNQIGAVYSLEEER
                     GKGYAKAAVSELCERIIGRGKLPTLIVSKQNIPALNAYKALGFEHYSDYLIVKLFI"
     gene            complement(3995..4543)
                     /locus_tag="PKU88_10920"
     CDS             complement(3995..4543)
                     /locus_tag="PKU88_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69821.1"
                     /translation="MKQHITIENLMELNEYQRDRLNSLWNPVRYDLAAGFLCKDAENN
                     EYDIFPFVIGYVNIRETRSGYSMTLMNLEALRSWKVQEEEAQEETGAEEEFSEEDFVF
                     EYERPDIYNKEDCLPLLNIGQMIEILNKCAYGKGSFYINLRKDPKDCSIGRNIEEYID
                     YGTDYNGEELCGVLWTAIKEAL"
     gene            complement(4574..5329)
                     /gene="murI"
                     /locus_tag="PKU88_10925"
     CDS             complement(4574..5329)
                     /gene="murI"
                     /locus_tag="PKU88_10925"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPX69822.1"
                     /translation="MSIGIFDSGVGGLTVLKEAVKLLPGEDFLYYADTKHVPYGTKPK
                     DEVKGYIFHAADFFAEHKAEALVVACNTATSIAINDLRKKYSFPIIGMEPAVKPAVEK
                     ADDRRVLVFATPITVREKKLHDLVIRLDSEQTVDLHALPGLVELAERFIFDEETVTQY
                     LLDELSGYDLKEYGAVVLGCTHFVFYRNYFKRILPHGVEVIDGNTGTVNRLRALLESR
                     GLDYGRKGSLEFYRSGEKETDDSKYRRYLELDY"
     gene            complement(5632..6372)
                     /locus_tag="PKU88_10930"
     CDS             complement(5632..6372)
                     /locus_tag="PKU88_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPX69823.1"
                     /translation="MIEKIYLFAVVLASFFVKGITGFGNTLVMAPLFSFVVSNRFTTP
                     VDLLISIPTNSYLVWKNRKSINYRTVVPLSIMLLTGVVPGIMLLKTGNDLLLKSILGV
                     VIMALGIEMLYRKPGHKGLAKPNKLFLMTVGLMSGVLAGMYGISAFLVAYISRTSEDR
                     SQFRGNLCSLFLVDNIFRLFWYSAAGIMTMEIVKFTLFILPAVALGMYIGTRVDTGLK
                     EETVRKATIYLLIVSGAALLLRSIYSTL"
     gene            complement(6385..7092)
                     /locus_tag="PKU88_10935"
     CDS             complement(6385..7092)
                     /locus_tag="PKU88_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPX69824.1"
                     /translation="MNLNEELYKLARSCMISYFGVADLSKVEHAAIDQAGTGIGDYPY
                     CVTMGIPLPKDIVDRLSYREHYNDALNYKLFAYDVINQRLDLAASMLASLIQEKGYRA
                     LPIPASGIIDHDRIYGAFSHKLGARLCGFGWIGKNCLLITSQNGPRVRWVSILTDSPL
                     QPTGEGIMEPRCGECTACADICPVNAIKGKTFTENESREARFDAHKCEAYFNSLTSAG
                     KLYVCGMCLYACPHGRK"
     gene            7234..8064
                     /locus_tag="PKU88_10940"
     CDS             7234..8064
                     /locus_tag="PKU88_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPX69825.1"
                     /translation="MVEYLSQFNLICLGIMASLGAGLATGIGSIPVFLTKKISKKLLD
                     LLLGTAAGIMLAATSFSLIIPAIEKGGGGIKGASTALVGILFGGVFIDFIDKFFPDTN
                     LLLNALNGNGNNAHEVNKSETAEINSRLRKVWLFVIAITVHNFPEGLAVGVGFGDGDI
                     MNGLCLAIGIGLQNLPEGLAVALPLLRENYPRGKAFLVALCTGLVEPIGGLLGIVLVQ
                     ISRPLLPFALAFAAGAMLFVISHEIIPETQHQKCHSKLATHALLVGFVVMMFLDNTLG
                     "
     gene            complement(8098..9006)
                     /locus_tag="PKU88_10945"
     CDS             complement(8098..9006)
                     /locus_tag="PKU88_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPX69826.1"
                     /translation="MESWEKIKAVQRMQSYIEEHLAETVTLNMLGKAAGYSPWHSERI
                     FKELTGKTPFEYIRALRLSRAAVKLKDGNTRVIDVAFDFVFDSHEGFTRAFSKQFGVT
                     PKYYSKNTPEIKLFMPGRIRDYYLRLQKGEDNMCKNPNTNAVFVQVVDRPARKLIFKP
                     GVKATHYYEYCEEVGCDVWNVLSGIKEAIYEPIGMWLPDNLRRPGTSTYAQGVEVPAD
                     YTGKVPEGYELMDLPPCKMMVFQGEPFDEEKFEEAIGNLWEVMKKYNPELYGFKWADE
                     DGPRFQLTPMGYRGYIEARPVRQLNA"
     gene            complement(9183..10067)
                     /locus_tag="PKU88_10950"
     CDS             complement(9183..10067)
                     /locus_tag="PKU88_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX69827.1"
                     /translation="MTNKTKADLMLLAVTVFWGASYMLTKIALDVLEPFNLSALRFLI
                     AFVVSALVFYKNILKSDIRIIKYSLILAVILVGMFISMSFGLKYTTASNAGFLISLAV
                     IIIPILSYVFLKQKIEKRVLIGVCIAPIGIALLTLNSQLSVGSGDLLCILCAFLSALH
                     VVVMGVFTRKVDSIALGVLQLGFAGILSIVFSLFTETLRLPSTGISWGATLALSILCT
                     AFGYIVQATAQQYTSATHTGLILSLEPVFSAILGFAFLGEILSPRGYFGAAVLLLSVL
                     IAEVGFRKKDVKAKEIDV"
     gene            complement(10456..11064)
                     /locus_tag="PKU88_10955"
     CDS             complement(10456..11064)
                     /locus_tag="PKU88_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69828.1"
                     /translation="MISIERLLALEKEDLPEFSKTLQKEEISQLMEWLTEKDDKLRYH
                     TLLLLQYRSEHYDDVYPFWDVFCEKLKSPNSYHRSLGLMLMADNVKWDRENKIDGAID
                     DYLKILYDEKPIIVRQCIQALGKIVPYKTQLHEKIANELMAINIMDVKQTMRKLILLD
                     ILNILALIRKNRTSEEIDGYISKALTGELLDKKSKKQIESML"
     gene            complement(11128..>11604)
                     /locus_tag="PKU88_10960"
     CDS             complement(11128..>11604)
                     /locus_tag="PKU88_10960"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPX69829.1"
                     /translation="MARKTYNEKLNNSGDLPKIESIGYDNKMAKRFGCGNMLIAAPLE
                     YDEVMKKIPEGKLITSDEIRAYLAKKHGADFTCQLTAGIFINIAANASRERENGGGKD
                     ITPYWRTLKKGGELNEKYPGGIELQKALLETEGHTVVARGKRYFVKDYAEKLCWLE"
     assembly_gap    11605..11704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11788..12333)
                     /locus_tag="PKU88_10965"
     CDS             complement(11788..12333)
                     /locus_tag="PKU88_10965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HPX69830.1"
                     /translation="MKRALLVIDVQNEYFTGKLPVTYPSDSLENILKAAEAANSNNID
                     IVIIQHTAPQKESLTFVRGSKEWELHPQIKAIKYSHYVEKNLPGSFTNTDLEKWLRER
                     NIDTITICGYMAQMCCDTTARQGLHLGFKVEFLFDAIGTLDISNYAGKIAAEELHKAV
                     LITQAMRFSKVLSAEEWIETL"
     gene            complement(12454..12786)
                     /locus_tag="PKU88_10970"
     CDS             complement(12454..12786)
                     /locus_tag="PKU88_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPX69831.1"
                     /translation="MSKIFSDSIINLGEADIPFEGAKAYLSQGDNHQILFMEFDKDID
                     LPEHSHGSQWAVVLEGRIDLTIGGIRKVYQKGDRYFIPEGVKHYGKIYAGYADITFFG
                     ESGRYKKK"
     gene            complement(12828..13400)
                     /locus_tag="PKU88_10975"
     CDS             complement(12828..13400)
                     /locus_tag="PKU88_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPX69832.1"
                     /translation="MGKKIQTLGCCGLDCGLCPRFYTEGSSRCPGCCGVDFENKHPSC
                     SFITCCVKKKGLEVCGECNEFPCRKFDKETGEADSFITHRRVMQNQRLIRESGIAAFL
                     EQQNRRMNFLNTMLECYDDGKSKSFYCLSAALLSLKSLDESLIKADSEIAEKPIGKED
                     FKSKARVMKEILNQFADEEREELKLRKAKK"
     gene            complement(13415..14179)
                     /locus_tag="PKU88_10980"
     CDS             complement(13415..14179)
                     /locus_tag="PKU88_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPX69833.1"
                     /translation="MGEVEEKLAKSLTGETTELIPYLPYLLQDLWELGSDPKEIIKLI
                     RKNIRKVKDLKILDLACGKGAVSVKLAKELGCHVQGIDIIPEFISYARQKAIELGVSD
                     LCSFSAEDINISVAGERGYDIVIMGAVGEVLGSPEETIQKLMNTIKPDGFIIIDDAYR
                     REGASGNYPSREQWLKLFEDEGIQMIEEKPAKEDELADINDKNNEFIINRANELKNKY
                     PDKAAMFDGYVNSQLAECHELENDIICVTWLLKKNS"
     gene            complement(14252..>14441)
                     /locus_tag="PKU88_10985"
     CDS             complement(14252..>14441)
                     /locus_tag="PKU88_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69834.1"
                     /translation="YRFLFYQGYNTVPDNEAYCKGLINDIDRFIGIRPRIKPWAGFSY
                     CIRTVKAREYFIIIISVI"
BASE COUNT         3979 a         3421 c         2458 g         4483 t
ORIGIN      
        1 atcccccact ccaaggcagg ttgcccacgt gttactcacc cgtccgccgc taagatttct
       61 ttggcactca atactctaat tcttcttccc tctctgtgct ggcactttaa aaacgcgtga
      121 tgaagaaact tttatcagtg tttttaacat ctatgttgtt tcttcatgac gcttcctttg
      181 cgtcactact agagtactca gtgccaaaga aatctccgct cgacttgcat gtgttaggca
      241 cgccgccagc gttcgtcctg agccaggatc aaactcttaa gttaatattt gattcgcttt
      301 cgcgaattct aactttttca tttgccctaa ctcaaaagaa ttctgactgt tttcagcgca
      361 gattgctctg cacttcaaac tttcatttgg ttatcatttt acactgttta gttttcaagg
      421 atcaatcctc atatattata tggaagatct tgcgattctt ctcatatcct gccggcgaat
      481 cagccgccga cctttactat gttatcacac cacttttctg ttgtcaatac tgatttcgct
      541 ggtttttcgt ggtaattcgc atccctcgca ttcgtaattt ggaacaacgt ttaagaatat
      601 atcatataag ttccagtgag taaagcacac cctgccgcta attttcacgg ttaatattca
      661 atattgttgg ttatctcctt aactctcatc atagctttac attatgtaaa cttataatcc
      721 gtaatttaaa tcatacgtaa ttatattttc ctgcgcacat tattatatgt aggaaaattt
      781 gaacagtcac atgttatttg ctcaccaata aaaatttgta cagaaaaaat gcgccccaag
      841 acgcattcga ttctattatt acaaatacaa tatgtatctg aacatcatcc agaagtgaca
      901 aaagcttccg tacaatacaa aaatatgaaa catctcatga aacccgaaaa cttttgattt
      961 cagcttcggc catttggtgc cgtaaataac tgcgccaaca gtgtacaata aacctccggc
     1021 aataagccaa agcattgccg ctatcggcat tgtcctgaca agcggtacaa atgcaattac
     1081 tataagccat cccatcaaca gataaaaaag ggtatacagc catctgggcg cattgaacca
     1141 cagtactttt aatacgattc cggccattgc aataccccaa atacttatga gaaggctcca
     1201 tccccagcct cccctcaatg gaataaggca taccggtgta tatgttcccg ctataagtat
     1261 ataaatcatc atatgatcta tctttctcag aaccctggtg cttttctcgg ataccgacag
     1321 caggtgatat atggaacttg ccgtatatag aagtatcagg cttgctccga atattgaaaa
     1381 ggatacaata tgccacactg tcccattcat cgaagcatag cgtatcaata agcaaagccc
     1441 cgcaaccgac agaatcgccc ctgcaaggtg tgaaaatccg ctgaaagggt ctcttacttt
     1501 cattttcata taatatctcc tcttctactt aatgcattcc ccatatagta tttactacta
     1561 tgtgttgtca tatttatatt atatattgta gtctttatat tttcaattca tatttctaat
     1621 ctttgtgctt catttttatg gtatactata tctcagaggt ttgtctgaat aaaactcata
     1681 gtgaggagat ggttaaatgg attttaatga agattccttc ttcaaaatag tggaggatat
     1741 atctcaggga agggatataa gaattccaga tattcctgac attgagcttt acatggatca
     1801 agtcactaca ttcattgata ataagctggg gtacaataaa agaattttaa aggacaagat
     1861 tctgacaaaa acaatgataa ataattatac caagtcaaaa atccttctgc cttccaaaaa
     1921 taaaaggtac aataggcagc atgtgatact ccttatactt atatattatt tgaagcaaat
     1981 tctgtcgatt aatgatataa gcatattgtt tgttcctttg tttaaaaata ttacttcctc
     2041 caatgacggg gcgggctatc ttgagaactt gtacaacgcc tttcttgaag taaaagataa
     2101 taatgcaaat gatctgaaaa gcattctttc tcaaaagctt gatctaatta gtaccaaggc
     2161 ggaggaattc cccaatgact caaaggaaac ctcaaaaatt ctgctaaccg ttctaatgct
     2221 ggtagcaaca gcaggtatcc agaaaagaat tgctgaaaaa ataattgacg attatctgac
     2281 aaaaaaatga ggccgtattt tggcctcatt ttcttattcc ctcacatgct catggtttct
     2341 cacatgatcc atgcagtatt cgtaatcccc ttcacattgg gggcagtatc tgaaatccat
     2401 tttggggtca tccttttcag tcttgccgca gatcgtacat ttatgaattg taaaatccct
     2461 gggcagctca gattggaatc ttctcctgtt gttgtacacc tgcctgctgc tttttgtccc
     2521 ggcaataata tccttcccaa agaacagaaa gtagttcatt atcgagacta ctgcaatgag
     2581 ttttgcggat aaatctccaa ccagtaccgt atagattata aaagcccaat tcagcatcgc
     2641 caggtatttc attttgaccg gtataacaaa aaacagaagc aattcaaaat caggatatat
     2701 tttcgcaaat gccagaaata atgataagtt cagatatagg gccgtggctc catatcccgt
     2761 tataaatgcc gcaatagtcg tacctatcat gcctgtaaaa tagaaaatat taaatttgaa
     2821 gcttccccat tcctgttcaa gtgcgcttcc gaccatataa tagaagtaca gcgtaaacag
     2881 aatccaaata attgatgtat taggcggtat gaatatataa gtcacaagcc tccatacctc
     2941 tcccctcaat accagctcag gtatgagcat cagcttgctt atgacaaacc cgcttctgtc
     3001 aaaatatgat aatgcgtata caatgcctgt cataaacaca atatatgaca tcaggttttt
     3061 aattgcaaat ctgcccaatc ttctctcaag tttatccagc catttcaaat gtatttcccc
     3121 cttaatccca caagtattaa tcatatgaaa agttttacaa tcagataatc gctgtaatgc
     3181 tcaaagccaa gagctttgta cgcattcaaa gccggaatgt tctgtttgct tacaataagc
     3241 gtcggaagct ttcccctgcc tattatcctc tcacacagct cagaaaccgc tgccttggca
     3301 tagccttttc ctctttcctc ttcaagggaa taaactgctc ctatctgatt cgtattatct
     3361 gtgaaggttt gtatgcaggc ctgcgccacc agtttattat ccacggataa tattataaac
     3421 tcttcctctt catttttttg aagaagtaat atcctcgtgc cctctatggt ccgctctgcc
     3481 ccgaagccgc gccaatatgc tttcctaaca aagcttatta tgcttgtatc atcatttcca
     3541 gatacatctg tgaacactgc tccttcaagc ttgaaaggct tgaaattcct gttgatctga
     3601 tagctgcatt cttcaaacct cttcaattcc ttgtaattct ttatagcttc gaaaagaggc
     3661 cttacgattt gatcttttcc caaaagcacg ttgaacctgc ccattatcat cagtttggaa
     3721 aggaatggta cagccccgac tgactcataa tgcggaatgc aggttcccag attgtaaaac
     3781 accaagatgc cccttaagtt gtcgccctcg aaatatccgt aataatcgcc gcagcgcagt
     3841 acatccttcc tattggtaat gccgcagtgc agaatatttc cgaaaacaaa agctgcttca
     3901 tcttcattcc tgctcaaata atccagtatt atagccctat cccactcatt taaaggcctt
     3961 atcaaaatac atcaccccta tatgctctta atttctacag agcctcttta attgctgtcc
     4021 atagaacacc acacaattct tcaccgttat aatccgtccc ataatctatg tactcttcaa
     4081 tattcctgcc gatgctgcaa tcctttgggt cttttcgcaa gttgatataa aaactccctt
     4141 tcccgtatgc gcatttgttc agtatctcta tcatttgccc tatgttaagg agaggcagac
     4201 aatcctcttt gttatatatg tcaggccttt catactcaaa tacaaagtct tcctcgctga
     4261 attcctcttc cgccccggtc tcttcctgag cttcttcctc ctgaactttc caacttctaa
     4321 gagcttcaag gttcatcagc gtcatggagt aaccggatct tgtctctctt atattcacat
     4381 agccaataac gaatggaaaa atatcgtact cattattttc tgcatccttg caaagaaatc
     4441 ccgccgccaa atcatatctg acgggattcc aaaggctatt gagcctgtcc ctctgatatt
     4501 cgttaagttc cataaggttt tcaatagtaa tatgctgttt cataagaatg cctcctcgta
     4561 ttggttttat tatttagtaa tcaagctcca gataccttct gtatttgctg tcatcggttt
     4621 ccttctctcc tgaacgatag aattccaaac ttccctttct gccataatcc aatcctctgc
     4681 tttccagcaa tgctcttaat cggtttacag tcccggtatt gccgtctatt acttcaactc
     4741 catgaggcag tatccttttg aagtaatttc ggtaaaatac aaaatgggtg cagccaagaa
     4801 cgacagctcc gtattctttc aaatcgtatc cggaaagctc atccagcaag tattgagtta
     4861 cagtctcttc atcaaatatg aacctctctg caagctctac cagtccggga agtgcatgca
     4921 aatcaaccgt ctgctctgaa tccagcctta taaccaagtc gtgcagcttc ttctccctga
     4981 cagtaatagg tgttgcaaac acaagcaccc ttctgtcatc agccttttct actgcaggct
     5041 ttactgccgg ctccatacca attatgggaa aactgtactt ctttctcagg tcatttatag
     5101 caatgcttgt agctgtattg catgccacaa ccagagcctc ggctttgtgc tccgcaaaaa
     5161 aatctgcagc atgaaaaata tatcctttga cttcatcctt tggttttgta ccataaggaa
     5221 catgcttagt gtcggcatag tataagaaat cttcaccggg aagcagcttc actgcctcct
     5281 tcaatactgt caggcctcct acgccggaat caaaaatacc tatactcata tatatctcct
     5341 caaatcttgc aaaattaaac tacacacttt atcatcagtt ggggtcatct ccactgatga
     5401 tatattatat gccttgtggt gaattttcat cagacaatat ttattaattt tccaataaat
     5461 tctaaataat atacaggttc aagtaccttg taacatctaa ttgttgcgtt atgatacgca
     5521 ggatatttgc ttccaataca aggcgatggg aagaggcgta gcagtggcta cggcgattga
     5581 catcaacgca gtagtgggac aaatagacaa gtatcatgca gcttttaggt gttacaaggt
     5641 actatatata cttctcaata gcagagccgc tccgctcaca ataagaaggt aaatagtcgc
     5701 tttcctcact gtctcttctt taagccccgt atccactctc gtacctatat acattcccaa
     5761 ggccactgcc ggcagtataa ataaagtgaa cttcacaatc tccatcgtca tgattcctgc
     5821 tgccgagtac caaaaaagcc tgaatatgtt gtcaacaagg aaaaggctgc acagattccc
     5881 tcggaactga ctcctgtcct cactggtcct gcttatgtat gcaacaagga aagcactgat
     5941 tccatacatg ccggccagta ctcccgacat cagccccacc gtcataagaa acagcttgtt
     6001 gggtttcgct aatcctttat gtcccggctt tcgatacagc atctcaatac caagtgccat
     6061 aataacgact cccagtattg attttaacag caggtcgttc cctgttttta gaagcattat
     6121 acccggtaca actccggtaa gcagcattat cgataggggc accacagttc tgtaattgat
     6181 gctcttcctg tttttccata caaggtatga attggtaggt atgctgataa gcaagtcaac
     6241 cggtgttgtg aatctgtttg atacgacaaa ggaaaacaaa ggtgccatta caagggtgtt
     6301 cccgaagccg gtgatacctt ttacaaaaaa ggaggcaaga actactgcaa acaaatatat
     6361 tttttcaatc aatgaacgct accttcactt tcttccatgg ggacatgcat atagacacat
     6421 tccgcacaca taaagctttc ctgctgatgt cagagagtta aaataagctt cgcatttatg
     6481 agcatcaaaa cgcgcttctc tggattcatt ttcggtaaaa gttttgcctt taattgcatt
     6541 tacagggcat atatcggcac aagccgtgca ttccccgcac cggggctcca taatcccttc
     6601 cccggtaggc tgaagaggcg aatcagtcag tatggatacc catcttaccc tgggcccgtt
     6661 ttgagatgta atcagcaggc agttctttcc tatccaaccg aagccgcaaa gccttgcacc
     6721 cagcttgtgg gagaatgcac catatatcct gtcatggtca ataattccgg atgccggtat
     6781 gggaagcgcc ctgtatccct tctcctgtat aaggctggca agcattgaag cagccaaatc
     6841 caatctctgg tttattacat catatgcaaa aagcttgtag ttcagtgcat cattgtaatg
     6901 ttccctgtat gacagtctgt ctactatatc tttcggtaaa gggatgccca ttgttacaca
     6961 gtaaggatag tctcctatac ctgttcctgc ctgatcgatg gccgcatgct caaccttgga
     7021 caaatccgcc actccaaaat atgatatcat acagcttctt gcaagcttat acagctcctc
     7081 attcagattc atgaatctgt tccctcccta tacgctcttc tcttaattat atataacaat
     7141 gcataataat atcaagctat atggagtacg gctgtaacaa tattattgct gaatcataat
     7201 atgaaatagc gggtattgta aggaggtcca tatatggttg aatatctctc acaattcaat
     7261 ctgatatgtc tgggaataat ggcaagcctg ggtgccggcc tggccacagg tatcggctcg
     7321 atacctgtat ttttaacaaa aaaaatatcc aagaagctgc ttgatttgct gttgggtaca
     7381 gctgccggaa taatgcttgc cgctacatcc ttcagtctga ttatccctgc tattgagaaa
     7441 ggcgggggag gaataaaggg cgcatcaaca gcccttgtcg gaatactgtt cggaggtgta
     7501 tttattgatt tcattgataa attctttccg gacaccaacc tgctcctgaa tgcgttaaac
     7561 ggcaacggta ataatgctca tgaggtaaat aaatctgaaa ctgcagaaat taattcacgg
     7621 ctccggaagg tatggctgtt tgtaattgca atcactgttc ataacttccc cgaaggcctt
     7681 gctgtgggcg tgggctttgg agacggcgat ataatgaatg ggctgtgtct tgcaatcgga
     7741 ataggcctgc agaacctgcc cgaaggcctt gccgtggccc ttcctttgct gcgggaaaac
     7801 tatccaagag gcaaggcttt tctggtggct ttgtgtacgg gtctggtaga gcccatcggc
     7861 ggattgctgg ggattgtcct tgttcaaata tccaggccgc tgctgccttt tgccctggcc
     7921 tttgctgcag gagccatgct gtttgtcata agccatgaaa taatacccga aacgcagcat
     7981 cagaaatgtc attcaaagct agccacccac gctcttttag tgggatttgt ggtaatgatg
     8041 tttctggata atactttagg ctgagggagt tctataaaga actccctcaa taattaatta
     8101 tgcattcaat tgcctgaccg gccttgcttc aatatatccc ctgtagccca tcggtgtaag
     8161 ctggaacctc ggtccgtcct catcagccca cttaaatccg taaagctccg ggttatactt
     8221 cttcataact tcccacaggt ttcctattgc ttcctcgaat ttctcctcat cgaagggctc
     8281 tccctggaat accatcatct tgcacggagg aaggtccatc agttcatagc cttccggcac
     8341 ttttcccgta tagtctgccg gcacctcaac cccctgtgcg taggttgacg tacctggcct
     8401 gcgcagatta tcaggcagcc acattcctat aggctcataa atagcctctt tgattccgga
     8461 cagtacattc catacatcgc agcccacttc ctcgcagtat tcataataat gagtcgcctt
     8521 gactccgggc ttgaatatga gctttcttgc cggtcggtcc accacttgaa cgaaaacagc
     8581 attggtattt ggatttttac acatattatc ctctcctttt tgtaatctga ggtaataatc
     8641 acggatgcgc ccgggcataa aaagcttgat ttccggcgta ttcttgctgt agtatttcgg
     8701 agtcacgcca aactgcttgg aaaaagctct ggtaaatcct tcgtgggagt caaatacaaa
     8761 atcaaaagcc acatcaatta cccgtgtatt tccatccttc agcttaacag ccgctcgaga
     8821 caacctcagc gcccggatgt actcaaaagg tgtcttaccg gtcaactcct tgaaaatcct
     8881 ttcagagtgc cagggtgaat atcctgcagc tttccccagc atattcagag taactgtctc
     8941 tgccagatgc tcttcgatgt aactttgcat tcgctgaacc gcctttatct tttcccagct
     9001 ttccatgatt tcacctccta gatatttcgc gaaaaacatt ttacacatat aattcagatt
     9061 tcgcgtaaat atttccttga tctcattgaa ttcgcgctat accctgatga aataccttaa
     9121 gcgcgttcaa tataagtaga ggatatcaat tataattagt tatttcttga cttgttttgc
     9181 tgtcatacat ctatttcctt cgcttttaca tcctttttcc taaagcccac ctctgcaatc
     9241 aagacgctga gcaaaagaac cgccgcaccg aaatatcctc ttggagacag tatctctcca
     9301 aggaaagcaa aacctaaaat tgcggaaaaa accggctcca gggatagaat aagccctgta
     9361 tgggttgcac tggtgtactg ctgtgctgta gcctgcacta tatatccgaa tgcggtgcac
     9421 agaatgctga gggccaatgt agcaccccag gatatgcctg tgctggggag cctcaatgtc
     9481 tcggtaaata atgaaaacac tatactgagt atgcctgcaa aaccaagctg cagaacaccc
     9541 agggcaattg agtcaacttt tcttgtgaaa actcccatta ccacaacgtg caaagcggaa
     9601 agaaatgcac acaatataca gagcaagtct ccgctgccta cgcttaactg tgaattcaat
     9661 gtcaataaag ctatgcctat aggagctatg catactccta tcaacactct cttctcaatc
     9721 ttctgcttca gaaatacata tgaaagtatt ggtatgatga ttactgcaag gcttatcagg
     9781 aagcctgcat tggaagcagt ggtatatttc aagccgaagg acattgatat gaacatcccg
     9841 accagtatta ccgccagtat caaagaatat ttaattattc ttatatccga cttcaaaata
     9901 ttcttataga atacaagggc agatacaaca aaagcaatca gaaagcgcaa tgccgacaga
     9961 ttaaagggct caagtacatc aagagcaatc tttgtaagca tatatgatgc tccccagaaa
    10021 actgtaactg caagcagcat caagtccgct tttgtcttgt tagtcaaagt aatctcctcc
    10081 caagcaaaaa gaccatggca ttccatgatc ttaaaatatt gcgtcacgga tgcattggcc
    10141 cgtgactgta atatgaaaca ggcactttat ttacaatcct gggccgtaaa gtactatttt
    10201 tgattttgct cttattaata tagatttcat tcttacggcc tcccgattct tccagctttt
    10261 cttcactatt aatataacat ggtacggtat aggaagcaat acaaaattta ccaactgcca
    10321 ttgataatat gccagtataa gacctccaag gactgactat ggaatattaa taaacgtggc
    10381 ttctatagtc caatcctcaa tatcaaatga aggtaacatt tttaccatgc ttcatttgaa
    10441 tttcactcca ataatctaca acatcgattc tatttgtttt tttgatttct tgtcaagcaa
    10501 ttcacctgta agcgctttgg atatatagcc gtcaatttcc tcagaagtcc tgttttttct
    10561 gattaatgca aggatattaa gaatatcaag caatataagc tttctcatag tctgtttaac
    10621 atccataata tttatagcca taagctcatt agcgattttt tcatgcaatt gggttttata
    10681 aggaacaatt ttacctaatg cctgaataca ctggcgtact ataataggct tttcatcata
    10741 taagatctta agatagtcat ctattgcccc gtcaatttta ttctccctgt cccatttcac
    10801 attatcagcc ataagcatta gccctaaact tctatgatat gaattggggc tttttaattt
    10861 ctcacagaac acatcccaga aaggatatac atcatcatag tgttcagaac ggtattgcaa
    10921 cagaagtaat gtgtggtatc tcagcttatc atccttttcg gtcagccatt ccatcagctg
    10981 tgatatttct tccttctgca atgttttgga gaattccggc aggtcttctt tttctaatgc
    11041 caataatctt tcaatgctaa tcattatttc ttcaccttcc tatataataa aacacatcgt
    11101 catgtgttga tatgcggctt tttgaattta ttccaaccaa caaagctttt ctgcataatc
    11161 cttgacaaaa tatcgcttgc ctcttgcaac taccgtatgc ccttccgttt ccagcaatgc
    11221 cttttgcagt tctatacccc cgggatattt ttcgttcagc tctcctccct ttttcaaggt
    11281 acgccaatag ggggttatgt ctttccctcc cccgttctcc ctctcccggg atgcgtttgc
    11341 agcaatgttg ataaaaattc ccgcggtaag ctggcaggtg aagtctgccc catgcttttt
    11401 tgcaagataa gctctgattt catctgaagt aataagcttt ccctcaggga tctttttcat
    11461 cacctcatcg tactcaagag gcgccgctat aagcatgttc ccgcagccaa agcgcttggc
    11521 catcttatta tcataaccta tggattctat tttaggcaga tcccctgaat tattcagctt
    11581 ctcgttataa gtttttcttg ccatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnttttag gcagatcccc tgaattattc agcttctcgt tataagtttt tcttgccata
    11761 aatatacctc ccctttttct tttcacttta taatgtttct atccattctt cagcagacaa
    11821 aactttacta aaccgcatcg cctgtgttat caatacagcc ttatgcaatt cctccgccgc
    11881 tattttccct gcataattgg aaatatcaag tgttccgatt gcatcaaaca aaaattctac
    11941 tttaaagcca agatgcaatc cttgtctcgc agttgtatca caacacatct gtgccatata
    12001 tccgcaaatt gtgatagtat caatattcct ttcccttagc catttctcca aatctgtatt
    12061 tgtgaaactg ccgggaagat tcttttctac ataatggcta tatttgattg ccttaatttg
    12121 gggatgcagt tcccattcct tacttcccct aacaaatgtc aatgattctt tttgcggcgc
    12181 tgtgtgttgt ataatgacaa tatcaatatt attgctgttg gccgcttccg ctgctttcaa
    12241 aatgttctcc agactgtcag aaggatatgt aacaggtaat ttgcctgtaa aatactcatt
    12301 ctgaacatca ataaccaata atgctctttt catcactttc ataattccct tctaatagtt
    12361 ctatttgcac cattctgcag catactgtcc aagaaggttt atcattcttt ctaaaacccc
    12421 ttcagaaccc atgcccggta ctgtataaaa aactcacttt ttcttatatc tgccgctctc
    12481 tccaaagaaa gtgatatctg catatccagc ataaatcttc ccgtaatgtt ttactccctc
    12541 ggggataaaa tatctgtccc ccttttggta taccttcctg attccgccta ttgtcaaatc
    12601 aatcctgcct tccagaacga cagcccactg gctgccgtga gaatgctccg gcaaatcaat
    12661 gtccttatca aactccatga aaaggatctg atgattatcc ccttgtgaca gatatgcttt
    12721 tgcgccttca aatggtatat ccgcttctcc cagattgata atagagtccg aaaaaatttt
    12781 tgacataagc ctttcctcct gtaatttttg tcttaatcca aagcatctca tttcttcgct
    12841 tttcttagtt ttaattcttc cctctcttca tcggcaaatt ggtttaaaat ttctttcatt
    12901 actcttgctt tgcttttaaa gtcttcctta ccaataggct tctccgctat ctcactatct
    12961 gcctttatca acgattcgtc caggctctta agggaaagca atgcggctga cagacagtaa
    13021 aagctcttgc ttttcccatc atcataacat tccagcatgg tattaagaaa attcattctc
    13081 ctgttttgct gttcaagaaa agcagcaatt ccggattctc taataagcct ctggttttgc
    13141 ataaccctcc tgtgggttat gaatgaatcc gcctctcctg tctctttatc aaatttacgg
    13201 caaggaaact cattgcattc accgcagacc tccagcccct ttttcttaac acaacatgta
    13261 ataaaagaac aagagggatg tttgttctca aaatcaaccc cgcagcatcc ggggcatctt
    13321 gaagagcctt cggtgtaaaa cctggggcac aatccgcagt caagcccaca gcagcctaat
    13381 gtttggattt ttttacccat tgattattcc ctccttatga gtttttcttc aacagccacg
    13441 taacgcaaat aatatcgttt tccagttcat gacactcagc cagttggctg ttgacgtagc
    13501 cgtcgaacat tgcagccttg tccgggtatt tatttttcaa ttcatttgca cggttaataa
    13561 tgaattcatt atttttatca tttatatcag ccaattcatc ttccttggcc ggcttttctt
    13621 caatcatctg aattccttca tcttcaaata acttcagcca ttgttcgcgg gatggataat
    13681 tacctgatgc accctctctt ctataggcat catcaatgat gataaagcca tctggtttaa
    13741 tggtattcat aagcttttga attgtttcct ccgggctgcc gagtacttcc cccactgctc
    13801 ccataataac aatatcatat ccccgttccc cggcaacaga tatatttata tcctcggcac
    13861 tgaagctgca caaatccgat actcctaatt cgatagcttt ctgccttgca taactgatga
    13921 attcaggaat tatatcaatg ccttgcacat gacatcccaa ttcttttgcc agtttcacgc
    13981 ttaccgctcc tttgccgcaa gcaagatcaa gtattttgag atccttcacc ttccgtatat
    14041 tctttcttat aagctttata atttctttcg ggtctgatcc cagctcccac agatcctgaa
    14101 gcaaatacgg caaataggga atcagttctg ttgtttcacc ggttaatgat tttgcaagtt
    14161 tttcttctac ctctcccata ataattctcc cctcatggtt tatcaggcag caccagaaaa
    14221 tttcctctgg cttattctgg atgtttttct attatatcac acttattatt attataaaat
    14281 attcccttgc tttcactgtc ctgatacaat aagaaaatcc ggcccaaggt ttaatccttg
    14341 gccggattcc gataaaccga tctatatcat ttatcaatcc tttgcaatag gcttcattgt
    14401 cgggaacagt attatatccc tgatagaata ggaatctgta a
//
LOCUS       DAOOAB010000061        14120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_5581_length_14120_cov_15.596943, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000061 DAOOAB010000000
VERSION     DAOOAB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14120
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5581_length_14120_cov_15.596943"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1690
                     /locus_tag="PKU88_10990"
     CDS             101..1690
                     /locus_tag="PKU88_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HPX69835.1"
                     /translation="MPLELIKNPLKVSRIVGENVFSTVVEEDINVPDINPDLYKILAP
                     GATVQIRDCEVLNDKVVVNGQILLSILYAADDEGKPLNSMDVAANFSQGIEVPGARPR
                     MRESINTVIQHVDCYMINSRKLGVKVIMDMHCKVEDLFDLELASDVRGLSDIQVLREE
                     SSFKQVMGYNKDHYEFNEELNLAADAPAIGKILRSDSKVIIKDEKPIEGKVEVNGLLG
                     IDILYKSETEENQLQYQEFEVPFTQYIEIPAAEKNMECVTESTLQECRLEVNEDANGE
                     RKVINAYVMLGMGARVFNSIEQEVVADAYSPTNVIDIERNIFSLSEFVGKGRSNIVIK
                     ETVGIKHGDPEIEKVCCVNVLPIVNEVKLLDDRVLLEGMAECTFVYESSYSAEPMCSM
                     TEQIPFRHFMDIPGVRLGMPCTVKCSADNVNFSKINNEMIELRVVLGVYAEVMRHIEK
                     KLVENVEAVEGISIDYGRIPAVTIYMVQKGDTLWSIAKRYNTTVDALAKLNNIENPSK
                     VANGTKIMILKNIRMGKDNSL"
     gene            1843..2712
                     /gene="ispE"
                     /locus_tag="PKU88_10995"
     CDS             1843..2712
                     /gene="ispE"
                     /locus_tag="PKU88_10995"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPX69836.1"
                     /translation="MNTLAGRAAAKINLAIDVLRKRPDGYHDVLMIMQSVALFDTITV
                     RALKGDIKVTSNNDRLPADKGNIVYRAAEYLKIKYNVKEGALINIEKTIPIVAGLAGG
                     SADAALTLKLLNKAWNLRLSRNEILDAGKKLGSDVPFCIQGGTALAEGLGEKLTQLPG
                     IPECLILLAKPAVSISTREVYEGLKLEEIRERPDIKSMIRYIDGRNLEGIASCMCNVL
                     ETVTIKKCPQIEELKNKLMEYGALGSIMSGSGPTVFGVFKDSASAYNAYDYIKDMVSE
                     VFLVKTTNQNLDF"
     gene            2742..3437
                     /locus_tag="PKU88_11000"
     CDS             2742..3437
                     /locus_tag="PKU88_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPX69837.1"
                     /translation="MDNNFLELKLDNYKPLREVVFNSIRGAIISGVLKPGERLMEVQL
                     AEKMGVSRTPIREAIRKLELEGLVVMIARKGAYVADLSIKNITDVLEVRAVLEGLASG
                     LAALRMTEEEIKDLELTARHFEQAMNSNDVEGVIQTDIEFHEKIFKATRNEKLLQLTN
                     SLMEQVQRFRVMYLNKAIKSTNLIKEHYKIVEAISRRNREMAENIAKIHIQNAEKDMM
                     RLLGSSSGKNDNP"
     gene            3453..4172
                     /locus_tag="PKU88_11005"
     CDS             3453..4172
                     /locus_tag="PKU88_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPX69838.1"
                     /translation="MDVIVLAGEKKDTVNKAFYKIKDKYMIEYVVETLRKSESIDRIA
                     VVGPKDKLEPAIGGIVDIVVEGTDSIVSNILLALEYFPEANKILVVTSDIPMITREAM
                     EDFIARCNEKDVDLCYSIVDKKVNDQKYPGVRRTYARLWEGRFTGGNVFCFNPAVKDK
                     CKDFVEKMLEYRKSPAKMAGVLGFAFLLRLALGILTINAIERKCESLLGIRAAAIISP
                     YPEICNDVDRLSDLEYIEKYM"
     assembly_gap    4243..4342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4343..4590)
                     /locus_tag="PKU88_11010"
     CDS             complement(<4343..4590)
                     /locus_tag="PKU88_11010"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPX69839.1"
                     /translation="MNDSYCVIAFGNTHMAIKVQKTLNEKGFSINMMPTLRAITAGCG
                     LSIRFSSEDLTNIQKAVSEINIEPSLHQFYTVENNMGE"
     gene            complement(4611..5870)
                     /gene="saoX"
                     /locus_tag="PKU88_11015"
     CDS             complement(4611..5870)
                     /gene="saoX"
                     /locus_tag="PKU88_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263598084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HPX69840.1"
                     /translation="MNKRILACALAIMMLTVVLTGCQDSSLQKNVVKVDYDVNEEVAP
                     YLAKLPAIPDADKEFVIQMGYNDCDHMVAAIIGENAGIYKALGLNVVVTKSGQTAAAM
                     ASGDMVAGYSGFSSMIKNANKGAPVFMPAGNHLGGSKYFVIKDSVESAEEIIGSRITV
                     GESDMYSPYWLSYCEQVGIPIDYTKYQGFDMGADDAIVAMKAGQLDGFMCCDPFGSVA
                     EMEGIGKIIGTEWGAHVSDDLETGWGLHCGYFINTDFAEEHPELTTRLVLAHCLAIQY
                     MYLHPYNAGMMFADDFGVDPAVGLRTMYLKLCAEGRTLSWDINETNIKNYTDYWHSYG
                     IPEDKIPEVKDLPSFFNLKYLEACGIESFKSFMEDNKINEKFPIRDSYLAWLEKAEEI
                     DGIDHSSTVGKTVEKWNNNEIVSKLLK"
     gene            complement(5958..6734)
                     /gene="saoP"
                     /locus_tag="PKU88_11020"
     CDS             complement(5958..6734)
                     /gene="saoP"
                     /locus_tag="PKU88_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591054.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6129..6131),aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HPX69841.1"
                     /translation="MTDNRNFRNMVGKGYYFSFFIALLILATIWYFAAHRINKVFIFP
                     YLESVIGELFYACTDLHVLRSLGITLRRVLTGVYYATIIGFPLGMLMGYSPFVMRTIA
                     PFINSFRQIPTTSWVPLAIVWFGLGDGPTIFVIMFHAIFTIILNTVAGVQSISKDFYN
                     AARSMGAKTLDLVKDVVLPGSLSGLITGIRISLGAAWMSVVUAEFIATNSGFGYIIKQ
                     AQLDMITEKVMAYMILAGVLGFAQDRILLVIETFLIRWKR"
     gene            complement(6796..7965)
                     /locus_tag="PKU88_11025"
     CDS             complement(6796..7965)
                     /locus_tag="PKU88_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HPX69842.1"
                     /translation="MMTLPERFNEFAEARRNGFLKVMELKESGANICGSFCQYAPAEV
                     IYAAGLYKVGLCGKSNDPIATAETHLPANLCPLIKSSYGHALEQSCPYAYFSDIVVGE
                     TTCDGKKKMYEMLGKLKPMQVIHLPNVPEEERSLEAWSMEIKHFKEGLEERFDVEITD
                     EKLREEIKWCNKERIQAARIYELGKYDPPAITGLDMRNIMDGEQYMFDRYEKYEKLKQ
                     ILDECVEKWENGNGPYKPDEKPPRILISGAGLGGVVDKTIAVIEELGGAIVCYEGCSG
                     IVSRRRLVEESEDKDPITCIAEKYLEVPCAVMSPNHKRMEQVAATIEEWKIEGLINIT
                     LHSCLPFGVESCNIEKVCKKCDTPYLHIETDFSKGDEGQIRTRIEAFLELIMQRR"
     gene            complement(8036..8368)
                     /gene="saoD"
                     /locus_tag="PKU88_11030"
     CDS             complement(8036..8368)
                     /gene="saoD"
                     /locus_tag="PKU88_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HPX69843.1"
                     /translation="MRVAYVFESVYAREILENMIIPQLEKGDHGAEVAGMMFFFDNAY
                     MLHKDSDIAKRLAAISKKSGMLLMACDRCCYQREIDDKLIEPAGIGCFPNLYAALAGA
                     NINQVITL"
     gene            complement(8402..9274)
                     /gene="saoA"
                     /locus_tag="PKU88_11035"
     CDS             complement(8402..9274)
                     /gene="saoA"
                     /locus_tag="PKU88_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HPX69844.1"
                     /translation="MSISVNGITKEFKNRRERNSNNIVLKDISFNVIEGEFVTLLGPS
                     GCGKTTTLTIIAGFQGHNGGQILINGKEVSKPGPDRAFVFQNYALFPWMKVGENIMYP
                     MKMRKVPKRERKEKLKELLDIAQLSDYENYYVHELSGGMKQRVALLRALACEPQVLLM
                     DEPLGAVDFQMRKLLQIQLENILQKKNVTVLMVTHDVDEAIYLSDRVIIMSRDHGRIL
                     DDIKVNLPRPRDRKDDIYHNYINQLTEILKSALDGDVKNEEDEELLAFIYKNEKKNDV
                     AATKLAAVKEYNPC"
     gene            complement(9290..9823)
                     /gene="saoB"
                     /locus_tag="PKU88_11040"
                     /pseudo
     CDS             complement(9290..9823)
                     /gene="saoB"
                     /locus_tag="PKU88_11040"
                     /inference="COORDINATES: protein motif:HMM:NF040727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
     gene            complement(9839..10087)
                     /locus_tag="PKU88_11045"
     CDS             complement(9839..10087)
                     /locus_tag="PKU88_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69845.1"
                     /translation="MNINKRIYFVIVTVMIAGFIIISLLPKDTGRVDSRNALRIGAGD
                     DISGWLLKQIVEISRETGNSERIANSDFITEAYEFQDC"
     gene            complement(10090..10599)
                     /gene="saoC"
                     /locus_tag="PKU88_11050"
     CDS             complement(10090..10599)
                     /gene="saoC"
                     /locus_tag="PKU88_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HPX69846.1"
                     /translation="MNRKFHKSAMSIIIVLTIAFAARQYLNVKEEARKNEPTWMRDKE
                     FAAMASEDNIALQYFREKYPERAVLLACEEDINDDKLVDLLIIYKENVDTRLVAVCNT
                     GDGNWYISPEIPAPIENQTIRFKNIDNEAEMEFIVSGEKKGAVGYAVYRLIEGEIKDL
                     FGEGMEDCC"
     gene            complement(10615..11709)
                     /gene="saoE"
                     /locus_tag="PKU88_11055"
     CDS             complement(10615..11709)
                     /gene="saoE"
                     /locus_tag="PKU88_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HPX69847.1"
                     /translation="MIIEFWEFLGNIIYSAINMLNSASVWMVVSFGIAGIVHEYMSPE
                     KMRKSSVGSTKISGVLWTTLAGTGVPICSCGSTPLGISLYYSGAYLGPTLAFMTSTPV
                     LNPVAIILTWGLLGKEVAIINIITGLTVPVIVGIIGNKFAGKELYLEDLEDGVERKTL
                     IFEEPTHFERIKTGLKWSYTELAVTISKYAVSGLLIAGLIFNIVPQSMIQTYLGNPGF
                     ISLFGITVVAALMYVCAVGHIPFIAALVASGAAPGVAITFLMAGAATNIPELLTIYKT
                     IGKRAMIIFLVTLTVLSNVAGYVTNLLLMPGFKPILNYDATSRTVATANKLIFVQPEW
                     LQYVCTSILAAYALYSLYKLIKGEKAKLLS"
     gene            complement(11706..11897)
                     /gene="saoT"
                     /locus_tag="PKU88_11060"
     CDS             complement(11706..11897)
                     /gene="saoT"
                     /locus_tag="PKU88_11060"
                     /inference="COORDINATES: protein motif:HMM:NF040730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HPX69848.1"
                     /translation="MKSLEEQYKEQIQVKIYKVGEDFEYIKKYGPTTKSMLIINESRV
                     VTKLSKEAIRKAFEEALKD"
     gene            12165..12932
                     /locus_tag="PKU88_11065"
     CDS             12165..12932
                     /locus_tag="PKU88_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPX69849.1"
                     /translation="MYFTGIDIGSTSAKVVALNENNDILYKNVMPTGWSSTDTAEKIR
                     KDLSIGGIDGGNSKFIATGYGRISVPYADKTITEITCHGKGAAFLYDVDTCTVIDIGG
                     QDTKIISIAKGQVVDFIMNDKCSAGTGRFLEIMASTLGMDLAELCNMAKTGSGISISS
                     MCTVFAESEVVSLIGSGHSKKDIAYGIIESITNKVISLCGKHAATNNKFYLTGGLCES
                     DYIIESLSEKLGSKVISSPLGRYAGAVGAALSAKSLK"
     gene            12950..14113
                     /locus_tag="PKU88_11070"
     CDS             12950..14113
                     /locus_tag="PKU88_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HPX69850.1"
                     /translation="MSNIDLPSKFEEFADARRQGFLKVKEIKDKGGKIAGVFCTFTPS
                     EILDAAGLFSVGLCGMSEETIIDAEADLPKNLCPLIKSSYGFALTDKCPYTYFSDIII
                     GETTCDGKKKMYELLGEIKDTYIMQLPQGVDREYAHKMWESELYRLIKTLEEKFQVKI
                     TEEKLRKAAALRNKQRNLYNELFELGKLDPPPIKGFDIYKVIEGSGFSFDVEGNCMRL
                     RELIDNTKKAYEAGNRPVAAGAKRILVTGCPIGGVLDKVVNTIESNGGAVVCFENCGG
                     VKPVRHLVDENAESIISAIADRYLKIGCSVMSPNIRRMEMLPELLKEFKIDGVIETVL
                     QACHPYSVETRTIKKLVNDAGLPYMSIETDYSKSDLGQIKTRISAFLEMEPVR"
BASE COUNT         4279 a         2861 c         2879 g         4001 t
ORIGIN      
        1 tcatatattg cgcaacagcc cctaaaataa aaatgttggc ataaattcct tctcgtctca
       61 tataaattaa tagaaaaact atagtaggag gcggagaata atgccacttg aactaataaa
      121 aaacccactc aaggtttcca gaatagtagg cgaaaatgta ttcagcacag tggtcgaaga
      181 agatataaac gtaccggata taaatccgga tttatacaag atacttgccc cgggtgcaac
      241 tgtacagata agggattgtg aagtgttaaa cgacaaggtt gttgtaaatg gtcaaatatt
      301 attaagtata ttatatgcag cggatgatga aggaaaaccg cttaacagta tggacgtggc
      361 cgccaatttt tcacagggta ttgaagttcc tggcgcaagg cccaggatga gagaaagcat
      421 aaatacagta atccagcatg ttgattgtta tatgataaac tcaagaaagc ttggcgtgaa
      481 ggtaatcatg gatatgcact gcaaggttga ggatcttttc gatcttgagc tggcttctga
      541 cgtaaggggt ttatcagaca tccaggtgct ccgggaggaa agcagcttca aacaagtgat
      601 gggctataac aaagaccatt atgagttcaa tgaggaactc aacctggcag ctgatgcacc
      661 tgcgataggc aagatattaa gatcggacag caaggtaatt ataaaggatg aaaaaccaat
      721 cgaaggcaag gtcgaggtga acggactgct gggaatagac atactatata agtctgagac
      781 tgaggaaaac caattgcagt accaggaatt tgaagtgccg tttactcaat acatagagat
      841 ccctgcggcg gagaaaaaca tggagtgtgt gacagaaagc actcttcagg aatgccggct
      901 ggaggttaat gaggatgcca atggcgagag aaaagtaatc aatgcctatg tcatgctggg
      961 tatgggagca agggtattta acagcatcga acaggaagta gtggcggatg cttatagccc
     1021 gaccaatgtg atagatatag aaagaaatat attctcactc agcgaatttg tcggaaaagg
     1081 ccgttcaaac attgtaatca aggaaaccgt cggtattaag catggggatc ctgagattga
     1141 aaaggtatgc tgcgtaaatg tgctgccaat agtaaatgaa gtgaagctgc tggatgacag
     1201 agtactgctg gagggtatgg cggagtgcac gtttgtctat gagtcttcat acagtgcgga
     1261 gcctatgtgc agtatgaccg agcagattcc cttcaggcat ttcatggata tacccggtgt
     1321 gaggcttggt atgccgtgta ccgtaaaatg cagtgcagac aatgtaaact tcagcaagat
     1381 aaacaatgaa atgattgaat taagggtggt gcttggagtt tatgccgagg ttatgaggca
     1441 tattgaaaaa aagctggtag agaacgttga ggcagttgaa ggcattagta tagactatgg
     1501 caggataccg gcagtgacta tatacatggt tcagaaaggt gatacgctgt ggagcatagc
     1561 caagagatac aataccacag tggatgccct ggcaaaactg aataatattg aaaacccaag
     1621 caaagtggca aatggaacga agataatgat attgaagaat attcggatgg ggaaggataa
     1681 cagtttataa ttatggagag actttattag caggatgaat aattcctgct tttattttta
     1741 ttcccaaact tatcggtaaa aaaatgtaca agaaattaga aaatggtata taatgataat
     1801 ggggggattt ttgtatacat aaagcaacgg agcgtgattg tattgaatac actggcagga
     1861 agggcagctg caaaaataaa tcttgcaatt gatgtactta gaaaaaggcc tgacggctat
     1921 catgatgtat tgatgataat gcaatcggtg gcattgtttg ataccataac cgtcagggca
     1981 ttgaaaggtg acataaaagt tacttcaaat aatgacagat tgccggcaga taaagggaat
     2041 atagtatata gagctgccga atacctaaaa ataaaataca acgtcaagga aggcgcactg
     2101 ataaatattg aaaaaacaat acctattgta gcggggcttg caggggggag cgcagatgcc
     2161 gcacttaccc ttaagctttt gaataaggca tggaatctgc ggttatccag gaatgaaata
     2221 ctggatgcgg ggaaaaagct gggttcggac gtgccctttt gcattcaggg gggaactgcc
     2281 ctggcggaag gtttgggtga gaagctgaca cagcttccgg gaattcctga atgccttata
     2341 ctattggcaa agcctgctgt aagcatatct acaagagagg tatatgaagg cctgaagctt
     2401 gaagaaatca gagaaaggcc ggacataaaa agtatgattc ggtatattga cggaaggaac
     2461 cttgagggta tagcttcctg tatgtgcaat gtactggaaa cagtgacaat aaaaaaatgc
     2521 ccacagatag aagaattgaa gaataaatta atggaatacg gtgccctggg aagcattatg
     2581 agcggcagcg ggccgacggt gtttggagta ttcaaggatt ctgcatctgc atataatgca
     2641 tatgattata ttaaagacat ggtgagtgag gtttttttag tcaagactac caaccagaat
     2701 ttagactttt agtatcaaaa tgctaacagg gggtgataat aatggataac aactttctcg
     2761 aattaaaact ggacaactac aagcccttaa gggaggttgt attcaattca attaggggag
     2821 ctattatctc aggggtgctg aaacccgggg agaggcttat ggaagttcag cttgcagaaa
     2881 agatgggggt tagcagaacg cctataaggg aagccataag gaaacttgaa ttggaaggct
     2941 tggtagtaat gattgcccgg aaaggtgcat acgttgcaga cctttcaatt aagaacatta
     3001 ccgatgtgct tgaggtcagg gcagttttgg aaggccttgc ttcgggactt gcagcattaa
     3061 ggatgaccga ggaggaaatc aaggatctgg aattaactgc caggcatttc gagcaggcca
     3121 tgaattcaaa tgatgttgaa ggcgtaatac aaacagatat tgaatttcat gaaaagatat
     3181 tcaaggctac aaggaacgaa aagctgctgc agttgaccaa tagtctgatg gagcaggttc
     3241 aaaggttcag ggtaatgtac ctgaataaag ccatcaaatc aaccaatctt atcaaagagc
     3301 attacaagat agttgaagca atttccagga gaaacaggga aatggctgaa aatattgcaa
     3361 agatacacat ccagaatgcc gagaaggata tgatgagatt gctggggagt tcatccggga
     3421 aaaatgacaa tccgtaggga ggattgaact ttatggatgt tattgtgctt gcgggagaaa
     3481 aaaaggatac cgtaaacaag gcattttata aaattaagga caagtacatg atagaatatg
     3541 tggtggaaac cctcagaaag tccgaaagca tcgacaggat tgccgtagtc gggccgaagg
     3601 ataagctgga gccggcaatc ggcggtattg ttgatattgt cgtagaagga acggattcca
     3661 tagtatcaaa tatacttctg gcattggagt attttcccga ggcaaataag attcttgttg
     3721 tgacctcaga tatcccgatg attaccaggg aggcaatgga ggactttatt gcaagatgca
     3781 atgagaagga tgttgactta tgctattcca ttgttgacaa gaaggtcaat gatcagaagt
     3841 atccgggagt caggagaact tatgcgcggc tttgggaagg ccggttcacc ggagggaatg
     3901 tgttctgttt caacccggct gtaaaggata agtgcaagga tttcgtggag aagatgctgg
     3961 agtacagaaa aagccccgca aaaatggccg gagtacttgg atttgcattc ttgctgaggc
     4021 ttgcactggg gatactgaca atcaatgcaa ttgaaaggaa atgcgagtct cttttgggca
     4081 taagagcagc agcgattatt tccccttatc ccgaaatatg caatgatgtg gatagactca
     4141 gtgatttgga gtatatagaa aaatatatgt aagaagcagc ggggaagcag cagggacggt
     4201 tctttttgct ttcaaaagca aaaagaaccg tccctgctgc ttnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nntattctcc catattattc tcaaccgtat aaaattgatg
     4381 taatgatggt tcaatattaa tttcagaaac agccttttgt atatttgtta gatcctctga
     4441 agagaatcgt atagaaagac cacagcctgc ggtgatcgct ctcaaagtcg gcatcatatt
     4501 aatactgaaa cctttttcgt taagtgtttt ttgaacctta atagccatat gagtgttgcc
     4561 aaaggcaatt acacaatatg aatcattcaa agtgatgaca ctccttatta ctattttagc
     4621 agcttcgata caatttcatt attgttccac ttttctacgg tcttacctac agtgctggag
     4681 tgatcgatac cgtcaatttc ttctgccttt tcaagccatg ccaagtaaga gtcccgtatt
     4741 ggaaactttt cattaatctt attgtcttcc ataaaactct taaaactttc tataccacag
     4801 gcttcgagat attttagatt gaagaaggac ggcaaatcct ttacttcggg tattttatct
     4861 tccggtattc catagctatg ccagtaatcc gtgtagtttt ttatgtttgt ctcattgata
     4921 tcccaggata aagtacgccc ttcagcacag agtttcaaat acattgtacg aagacctact
     4981 gccggatcta cgccaaagtc atcggcaaac atcattcctg cgttatatgg gtgcaaatac
     5041 atatactgaa tggcaagaca atgtgccaac accaatcttg tagttaactc ggggtgttct
     5101 tctgcaaaat ccgtgttgat aaagtatccg cagtgtaagc cccaaccggt ctccaaatca
     5161 tctgaaacgt gggcgcccca ctcagtaccg attattttgc ctataccttc catctcagca
     5221 actgacccaa agggatcgca gcacataaag ccgtctaact gaccggcttt cattgcaact
     5281 atagcatcat cagcgcccat atcaaaccct tgatatttag tataatcaat tggtatacca
     5341 acctgttcgc agtagcttag ccagtaagga gaatacatat cactttcacc tacggttatt
     5401 cttgacccga ttatttcttc cgcagattcg acactatcct taataacaaa atatttggag
     5461 ccgcctaaat gattacctgc aggcataaat accggagcgc ccttatttgc attttttatc
     5521 attgaactga agcccgagta cccggccacc atatcaccgg aagccatagc ggcagcagtc
     5581 tgcccgctct tagtaacaac gacgttcaga ccgagagctt tgtaaatacc ggcgttttca
     5641 ccgataatgg cagcgaccat atgatcacag tcgttatacc ccatttgtat aacaaactct
     5701 ttatctgcat cgggaattgc cggaagcttt gcgaggtatg gagcaacctc ttcattaaca
     5761 tcataatcta cttttactac gtttttctgt aagctgctat cctgacatcc ggtcaatact
     5821 actgtcagca tcataatagc aagcgcgcat gcaagtattc ttttattcat ataaattctc
     5881 cctttctata tgttttgtat ctcatctatt tcctcacggg aaatcgtcac tgcaacataa
     5941 tatggaaaac ttccgtatta ccgtttccac cttatcagga atgtctcaat tacgagtaaa
     6001 atcctgtctt gtgcgaaacc cagaaccccg gccagtatca tgtaagccat aaccttttcg
     6061 gttatcatat ctaattgtgc ctgtttgatt atatatccaa agccggaatt tgttgcaatg
     6121 aactccgctc agactacgga catccatgcc gcacccagag aaatacgtat accggtaatc
     6181 agccccgaaa gggaaccggg aagcaccaca tctttaacca agtctaaggt tttggcgccc
     6241 atactgcggg cggcattgta aaaatctttg cttatacttt gcacccctgc taccgtattt
     6301 aatataatag taaaaattgc atggaacata atgacaaaaa tcgttggtcc gtcacctaat
     6361 ccaaaccata ctattgccag tggaacccaa gaagttgtag gaatctggcg gaatgaattt
     6421 ataaaaggtg caatggttcg cataacaaag ggtgaatagc ccatcaacat acctaaggga
     6481 aaaccaatga ttgttgcata ataaacccct gtcaagactc ttcgcaatgt tattccgagg
     6541 ctccgcaaaa cgtgcaggtc tgtgcatgca taaaataatt cgcctattac actttccaga
     6601 tatgggaata taaagacttt attgatccta tgtgctgcaa agtaccaaat agtagccaaa
     6661 atcaataacg caataaaaaa ggaaaagtag taacctttgc caaccatatt tcgaaaatta
     6721 cgattgtccg tcatcttggt tgcttgcagc atccgatcca tatccctatt tgcttcgtta
     6781 gccattactg ctacattacc tcctttgcat tatcaattct aaaaatgcct cgatacgggt
     6841 acgtatttga ccctcatcac cttttgaaaa atcggtttct atgtgcagat atggggtatc
     6901 acacttcttg cagacctttt ctatgttaca tgactcaacc ccaaagggaa ggcaagagtg
     6961 cagcgtaata tttatgaggc cttcgatctt ccattcctct attgttgcgg cgacctgctc
     7021 catgcgctta tgattcgggg acattaccgc acatggtacc tctaagtatt tttccgcaat
     7081 gcaggttata ggatctttgt cttcggattc ctcaaccagg cgtcgacggg aaacgatacc
     7141 gctgcagcct tcatagcaaa cgattgctcc tcccagctct tcgataacag cgatagtctt
     7201 atccacaacg ccccccagac cggcaccgga tatcaaaata cggggaggtt tttcatcggg
     7261 tttgtaggga ccatttccat tttcccattt ttcaacacat tcatccaaaa tctgtttaag
     7321 cttttcatat ttctcatatc tatcgaacat atactgctcg ccgtccataa tattgcgcat
     7381 atccaagccg gtaatagcag gtgggtcata tttgccaagc tcgtagatac gcgcagcttg
     7441 aatacgctct ttattgcacc attttatttc ttcacgaagc ttttcatcgg ttatttcaac
     7501 atcaaagcgt tcttccaaac cttccttaaa atgcttaatt tccattgacc aggcttctaa
     7561 tgagcgctct tcttccggaa cgttgggtaa atgaattact tgcatcggtt tgagctttcc
     7621 cagcatttca tacattttct ttttcccgtc acaggtggtc tcaccaacaa caatgtcaga
     7681 aaaataggca taggggcagc tttgttccaa tgcatgccca taacttgact ttataagggg
     7741 acacaaatta gcaggcaaat gtgtttccgc agttgctata gggtcattgc tcttgccgca
     7801 aagaccaacc ttgtatagtc ctgcagcgta aataacctct gccggagcat actggcaaaa
     7861 gctgccacaa atgttggcac cgctttcctt caattccatt accttcaaaa aaccgttacg
     7921 gcgggcttcg gcaaattcgt taaaacgctc gggcaatgtc atcataatat cttactcctt
     7981 aattcggcta attttaaaaa gaggcaggca taagctctgc ctctcctgac ggatactaga
     8041 gtgtaataac ctgatttatg tttgctccgg ccaatgcagc atacaaattg gggaagcagc
     8101 ctattcccgc cggttcaata agcttgtcat cgatttctct ttgataacag cacctatcgc
     8161 acgccatcag aagcattccc gactttttgc tgattgcagc cagtctttta gcaatatcgc
     8221 tatccttatg cagcatatag gcgttgtcga aaaagaacat catacccgct acttcggccc
     8281 catggtcccc tttctccaac tgtggaataa tcatgttttc aagaatctct cttgcataaa
     8341 ctgattcaaa tacatatgca actctcattt tcctgcctcc cattattttt atatataccc
     8401 tttagcaagg gttatactct tttactgcag caagcttggt tgctgcaaca tcattctttt
     8461 tctcattctt atatataaac gctaatagct cttcatcctc ttcattttta acatcaccat
     8521 ctaatgctga tttcaatatt tcggtcagtt gatttatata attatgataa atatcgtctt
     8581 tcctatctct cggtcgcggc agattaactt taatatcgtc caaaatcctg ccgtgatccc
     8641 tggacataat gattactctg tcacttaaat aaatcgcctc atcaacatca tgagttacca
     8701 tcaataccgt tacatttttt ttctgcaaaa tattctccaa ttgtatttgc agaagctttc
     8761 tcatttggaa gtctactgcc ccaagaggtt catccataag taacacctga ggctcacaag
     8821 ccaatgcacg aagcagtgca actctttgct tcatgcctcc tgacaattca tgaacatagt
     8881 aattttcata atcgctgagc tgtgcaatat ccaataactc tttaagcttt tctttccttt
     8941 ccctttttgg cacttttctc attttcatgg ggtacataat attttctccg actttcatcc
     9001 atggaaaaag agcatagttt tggaatacaa aagctctatc aggtcccggt ttgctaactt
     9061 ccttcccatt gattaatatc tgccctccgt tatgcccctg aaaacctgct attatggtca
     9121 aggttgttgt cttaccgcaa ccggaaggac caagcagtgt aacaaattct ccctcgatca
     9181 cattaaagga tatatccttt agtacaatat tattgctgtt tctttctctt cgatttttaa
     9241 actcttttgt aattccatta acagatattg acattatata cacttcctat tatagttgta
     9301 aaaactttat attaaccata tcccaaaact ctcttgtcat tccaaaggct gaaatcaatg
     9361 tttctttctt attgagttct tctgctgctt tgttataatt atcaagaaaa tcatcaaaga
     9421 tctctgtgcc gattaaatcc tttcgtacta cgaggcagta agaaatataa tcatccttcg
     9481 aaatagcggc aaagttatat cttggcaata ataccgcttt ggttacatca atagccgcta
     9541 tatctatctg atcattctcc aatgtatatg gtaaagccgc aggaatcatt tctttgcttt
     9601 cctttatatt ggagtagcta tcctgaatca actccagtat atgtttgcgt tcctgcccta
     9661 ttccgagcac ttgtttctcc tcgatattac ctttgtagac cataacttcc gaattcataa
     9721 tgaccggacc gtaaattacc aaacgatcac ttattttgac catatgcatt gctttatgat
     9781 tgcagtaaaa acccatatca atttcatctg agctcaaggc ccattgggag gtattacttc
     9841 aacaatcttg gaattcataa gcttccgtaa taaaatcact attggcaatt ctttcgctat
     9901 tacctgtttc tctgctgatt tctacaattt gcttaagcag ccaaccgcta atatcatctc
     9961 cggctccaat acgcaaagca tttctgctat ccaccctgcc ggtatccttg ggaagcaagg
    10021 agattataat aaaaccggca atcataactg taactataac aaaatatatc ctcttattaa
    10081 tattcatatc tagcagcagt cctccatgcc ctctccgaat agatccttga tttccccttc
    10141 tatcaagcgg taaaccgcat atccgaccgc tccttttttt tcaccggaga ctataaattc
    10201 catctctgct tcattgtcaa tattcttgaa ccgtatagtc tgattctcga taggtgcagg
    10261 tatttccggg gatatatacc aattcccatc acctgtatta caaactgcaa caagccttgt
    10321 atctacgttt tctttgtata taatcagcaa atccacaagc ttatcatcgt taatgtcttc
    10381 ctcacaagcc aataatactg ccctttcagg gtacttttct ctaaagtatt gcaaggctat
    10441 gttatcctct gatgccattg cagcaaattc tttgtccctc atccatgtcg gctcattttt
    10501 ccttgcttct tccttaacat tcaagtattg ccttgcagca aatgcaatag ttaaaacaat
    10561 gatgattgac atagcacttt tatggaattt cctattcata ttttcaacat ctttctatga
    10621 taatagtttt gccttttctc cttttatcag cttatacaag gagtataatg cataagctgc
    10681 caatattgat gtacacacat attgcagcca ttcaggctga acaaagatca gcttattggc
    10741 tgtggccacc gttcgggagg tggcgtcgta atttaagatt ggtttgaaac ccggcattaa
    10801 taaaaggttt gtaacatatc ctgcaacatt ggataaaacc gtaagtgtta caagaaagat
    10861 aatcattgct cttttgccga tagttttata tatagtcaga agctccggaa tatttgttgc
    10921 cgcgcctgcc ataagaaagg taatggctac tccgggtgcc gcgccgctgg ctacaagggc
    10981 tgcaataaag ggaatatgac ctacggcgca cacatacatc aatgcagcga caacggtgat
    11041 tccaaaaaga gatataaaac cggggttacc caaatacgtc tgtatcatag actgagggac
    11101 gatattaaaa atcaaaccgg caatcaaaag accggaaaca gcatatttgg agatggtcac
    11161 tgctaattca gtataagacc actttaaacc ggttttgatc ctctcaaaat gtgtaggttc
    11221 ttcaaaaata agtgtttttc tttcaacccc atcctccaag tcttccaaat aaagctcttt
    11281 acctgcaaat ttattgccga ttattccgac aattaccggc acggtaagcc ctgtaataat
    11341 attaataatt gcaacttctt tccccaataa tccccaggtc agaataattg caacaggatt
    11401 aagcaccgga gtggatgtca taaatgccaa agtgggaccc aaataagcac cggaatagta
    11461 taagctgatt cccaaagggg tgctgccgca gctgcaaatt ggtacgcccg tgcctgcaag
    11521 cgttgtccag agaacaccgg aaattttcgt actgccgaca ctgcttttgc gcatcttttc
    11581 cggactcata tactcatgaa ctattcctgc aataccaaag ctcacaacca tccatacaga
    11641 tgcactgttc aacatattga tagcagaata aataatattg cctaagaact cccaaaattc
    11701 tattatcaat cttttaatgc ctcctcaaat gcttttcgta tggcttcttt gcttaatttt
    11761 gtcactaccc tcgattcatt gattatgagc atgctcttgg tagtaggtcc gtattttttt
    11821 atgtattcaa agtcctctcc gactttatag attttcactt ggatttgttc tttatattgt
    11881 tcttcgagag atttcacgaa ttctccaaat acttcgcatc atgcacaagc actgatatac
    11941 tcgataaaag gcttattcat cgttcttccc cccctttaag ttgataaaga acaaaagcgg
    12001 cttcgccgcc aagaaagcct acacactcaa atatatcaat aaaagtgacc aatttctaca
    12061 aatattacca aatatgatag acagatattc cattataaaa aatagcaata atgtgtatta
    12121 gatatttcat gtataattta gtaggagaaa ggcaggagat aaaattgtat tttaccggga
    12181 ttgatatcgg ttcaacctct gcaaaggttg ttgccctaaa tgaaaacaac gatatacttt
    12241 acaaaaatgt aatgccaacc gggtggagca gcacggatac tgccgagaaa atcagaaagg
    12301 atctatcaat aggcggtatt gacgggggaa acagcaaatt catagcaacc ggctacggca
    12361 gaatctctgt tccctatgcg gataaaacca taacggaaat aacctgtcac ggtaaaggag
    12421 cagcattttt atatgacgtg gatacttgca cggtaataga tattggcggt caggatacaa
    12481 aaattatatc tattgccaaa ggtcaggttg tcgattttat tatgaatgac aagtgttctg
    12541 ccggtacagg ccggtttttg gaaattatgg caagcacatt gggcatggat ttggctgagt
    12601 tgtgcaatat ggccaaaacg ggctcaggta taagtatcag ctctatgtgt acggtttttg
    12661 ccgagtctga agtagtgagc ctgattggca gtggtcactc aaagaaggat attgcatacg
    12721 gaataataga atccatcaca aacaaggtaa tatcactgtg cggcaagcat gctgccacga
    12781 ataacaaatt ttatctgacc ggtggtcttt gtgaaagtga ttatattata gaatccctat
    12841 ctgaaaagtt agggtccaaa gtaatttcaa gtcccttagg tcgatacgcc ggtgcagttg
    12901 gagctgcact atcggcaaaa tctctaaaat aatattagga gggtatgtta tgtcaaacat
    12961 agatttgcca agtaaattcg aagaatttgc tgatgccaga aggcaaggct ttttaaaagt
    13021 aaaagaaata aaagataaag ggggtaagat tgccggcgtt ttttgcactt ttacaccatc
    13081 tgaaatattg gatgcagcgg gtcttttctc tgtgggatta tgcggcatga gcgaagaaac
    13141 tataattgat gccgaagcag accttccgaa aaatctgtgc cccctgatca aatccagtta
    13201 tggttttgca ttaacggaca aatgccctta tacttatttt tccgatatta ttattggtga
    13261 gacaacctgc gacgggaaaa agaaaatgta tgagctttta ggtgaaatca aggatacata
    13321 tattatgcaa ctacctcaag gggttgacag agagtatgct cataaaatgt gggaaagtga
    13381 attgtatcgt ttgataaaga ctttggagga aaaattccag gtcaaaatta ctgaagaaaa
    13441 gcttagaaag gctgcggctc tgagaaacaa acaaaggaat ctatacaacg agctttttga
    13501 attaggcaaa ttagaccctc cgccaatcaa aggctttgat atatacaagg tgattgaagg
    13561 ctcgggcttc agctttgatg tggagggaaa ttgcatgagg cttcgtgaac tgatagacaa
    13621 cacaaaaaag gcttatgaag ccggtaacag accggttgca gcgggagcaa aaagaatatt
    13681 ggtgacgggt tgtcccatag gtggagttct tgacaaggtt gtaaatacta ttgaaagcaa
    13741 tggcggagca gtcgtttgct ttgaaaactg cggcggtgtc aaacctgtaa ggcatttagt
    13801 ggatgaaaac gcagaaagca ttatcagtgc aatagctgac cgctatttaa aaataggctg
    13861 ctctgtcatg tcacctaata taagacgaat ggaaatgctt cctgaattac ttaaggaatt
    13921 caaaattgac ggcgtaattg aaaccgtttt gcaggcttgt cacccttata gtgtagaaac
    13981 aagaacaatc aagaagcttg ttaatgatgc cgggctgcca tacatgtcca ttgagacaga
    14041 ctattcaaaa tcagaccttg ggcaaatcaa aacaagaata tcagcatttc tggaaatgga
    14101 acctgtcaga tagccatagg
//
LOCUS       DAOOAB010000062        13028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_6166_length_13028_cov_12.919140, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000062 DAOOAB010000000
VERSION     DAOOAB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13028
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6166_length_13028_cov_12.919140"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1786)
                     /locus_tag="PKU88_11075"
     CDS             complement(<1..>1786)
                     /locus_tag="PKU88_11075"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPX69851.1"
                     /translation="YISTVDSGNFIGYLMTLRQGLLEYKNKPSVDSGMVLGLKDTSEL
                     VKLEDEQLRLPDEIFDKALEGGMLEQQGFIELLTKIIGKNDLKRYNWGIKLHKSAITF
                     LKEINSGISQENNEELMFKSEWLIGRIDKLIEETSFLPLFDNKRKLFSIGYNEEEEQL
                     TKSYYDLLASEARQASLISIARGEVKKEHWFMLNRTLTEVDGFKGLLSWTGTMFEYLM
                     PLLIMKDYSDTLLHETYDFVVRCQKDYGNKRKVPWGVSESGYYSFDFRLSYQYKAFGI
                     PSLGLKRGLINDTVIAPYATVLALMVNPKESFKNILTLKEEGLDSPYGFYEAIDYTPE
                     RLKRGKRSSIVRSFMAHHQGMSFMALNNVINNYILQERFHREPVIKAAEILMQEKIPS
                     KVIFAKDYKEKIEPFEVVVKEDVEYTKVLGLNNNILPEVHMLSNGDYSVMVTDNGSGF
                     SKYRDLAVTRWREDSVLNYSGMFFYLQDIDSGSVWSNSLAPCSVLPNKYKVVFSQDKI
                     CISRTDDDIDTNTEIIVSPEDDAEVRRLTLTNHSQQVRTLEVTSYLEVSIAHQSADLA
                     HPAFNNLFINTEYLHKYNTLLSVRRPREE"
     assembly_gap    1787..1886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1887..4504)
                     /locus_tag="PKU88_11080"
     CDS             complement(<1887..4504)
                     /locus_tag="PKU88_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPX69852.1"
                     /translation="MNLVANYFYMAAFVLAAVLIYLLSRLLYFIDRDEPEVYDAILNA
                     EELEKHALELARKHVVGKRAKHYFSLEGRMNKNFRFITSVYKDLNEYANERSSVTPAA
                     EWLLDNFYIIEEQVKEIRQNLSRKYYYQLPRLKSGFLKGYPRVYAIALELVSHTDGRF
                     DDKQLVGFVSAYQSQSLLSNGELWAIPIMIRMALIEHVRHVCESISLSQQQWHKAEKL
                     AEQILSYKDRKPEELLAIAKESMRQLENISPSYGEHLLNMLKKHGIEIAPIIHYIDER
                     LSEQHTTSESITQLEHKEQAARQVSMGNSITSLRFISTLDWKVIFEELSQVEQILRQD
                     PAGIYQKMDFASRDKYRHELEILSKKLKVSEIKVARKLIECSVEDNRHIGYYLFREQK
                     EQLLDRIARKGMYIYSPAGVYFFSIITLTAFLVFLMLNYSYREGAAGFWTQLVIALAV
                     LIPASDVSVSAINWILVHIKPPAFLPKLELKDGIPEDAAAIVVMPTLITKGSSAVKLL
                     LKLEEYYLANREGNLYFALLGDYKDSDGEKSEGDQEIILAASEKVRELNRKYGSDEDK
                     FFFFHRRRVFSKTQNRWMGWERKRGALYEFNELLLGGKKASFEHIEGNISALKDVKYV
                     ITIDSDTKLSIETAKKLIGTITHPLNKAVVDEGRGVVKEGYGLLQPRISVDIVSANTS
                     RFARVFAGQGGIDIYTNAVSDIYQDYFGEGIFTGKGIYELAVFQGILRNMIPENTVLS
                     HDLIEGAHVRTGLVTDIELIDGYPSKYSASSMRLHRWVRGDWQLLQWLFSKVRSAKGE
                     IVRNPLSPINRWKILDNMRRSLVPPNLVLLFFLSLWLMPGNAMVWLLLTLIAAVFPLF
                     VSFAEVVMTKYDRRMN"
     gene            complement(4636..7122)
                     /locus_tag="PKU88_11085"
     CDS             complement(4636..7122)
                     /locus_tag="PKU88_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPX69853.1"
                     /translation="MKAVIMAGGEGTRLRPVTCGIPKPMVPVLNKPVMEYTIELLKKH
                     NITDIAATLAYFPAVITDYFGDGGEFGVSLRYYIEHTPLGTGGSVKNAEDFYDDTFII
                     VSGDALTDIDFEKALEFHKYRRSKATLILKKVPVPLEYGVVIIDEEARITSFMEKPSW
                     GEVFSDTINTGIYILEPEVLDYYSKGDNFDFSKDLFPKLLRDNIPMYGYVTDDYWNDI
                     GDLRVYKEVNFDILSGKVKTTIGYRQHGEGIWIGDGCEISDSCNLIAPVLIGDNCRIK
                     DRTVIEASILGNDTEVQEATSIKKSIIWKNSSIGKNVQCRGGVICSGAAVKSGVHIFE
                     NTVIGSNTTLETGAIIKPDIKIWPEKVVEEDAVVAQNLVWGTKASRTLFGHRDVSGDV
                     NINLTPEFASRLGSAFGSILKDDSGIIVSSDGSNSAEIMKESLISGVLSTGVGVISIE
                     NAAVPIMRFAVRFYKAEGGVHIWKDYSDKNKVHLEFSGSKGISIDRSVERKIENSFIR
                     EDFKRCNADRIKDIISAGNFTDFYIQSGINMLKYTDRMRSSGIKLVVASISSQIAGLA
                     QMYLKNMGCSVERDSVWDRYKMPAKDNISAGAYYEHIAELTVKSGSCMGVVFSEDGES
                     FVLIDHKGRILDKDKQIALTSLIILKTGMDKRLIIPHTASRVIEEMAKCYSIEVVRTK
                     NTPAAIMREMFQQNTGMEESLFQYILNFDGIWGIGNLVDFMAGNNVTLYDLVNELPHF
                     HMEKNEVKCDWQHKGRVIREIIEENKNREIELFEGVRINNENGWVLILPDSERPVCNV
                     YAEGASEEYARELAAEFTDKIKKIVSAEDK"
     gene            7367..8683
                     /locus_tag="PKU88_11090"
     CDS             7367..8683
                     /locus_tag="PKU88_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX69854.1"
                     /translation="MSKPVLSAHFESRTPSSVRLAQMKYEERKVKPQAVINVGIGNVS
                     LPTNPAMQKRMFSLDAPESPFAKGVIRYTTTAGLIECQDAFKNILKCEGFDTSKLYVQ
                     ITDGGSAAMELLLLGVCGPAGTDEKPLMVIDPAYTNYISFAERVGRKIVTVTRSLNEN
                     GKFSLPKLNEIEETIKKYNPGALLVIPYDNPTGQFYDYETLKGLAALCVKYNMWFVSD
                     EAYRELFYEEGSKLVSIWGVTDADVPGIEGRRMSIETASKVWNACGLRIGALITDSPE
                     FNNRSVAEYTANLCANAIGQYIFGALAHESKEQIAAWCKSIREYYKELIFNVYNGLKK
                     QEPGLIVSSPDASIYSVIDVRNVVKPGFDATEFVLYCAGEGSVNLDGVETTLLVAPMK
                     GFYNTKKGEYNPGSTQFRISFVETPDKMAKVPELFAKLLRQYEAQR"
     gene            complement(8917..11556)
                     /gene="alaS"
                     /locus_tag="PKU88_11095"
     CDS             complement(8917..11556)
                     /gene="alaS"
                     /locus_tag="PKU88_11095"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPX69855.1"
                     /translation="MEKLGLNEVREKFLSFFESKDHLRMKSASLVPQSDKSLLIINSG
                     MAPLKPFFTGQAVPPSLRVTTCQKCIRTPDIERVGKTARHGTFFEMLGNFSFGDYFKD
                     EAIAWAWELCTEVYKMPEDRLWVTIYLDDDEAFDIWHNKIGLPPDRIVRMGKEDNFWE
                     IGLGPCGPCSEIYFDRGPGTGCGKEDCKLGCDCDRYMEFWNLVFTQFDKDEEGNYNRL
                     AKPNIDTGMGLERMAAVMQGVDNIFEVDTIKYILDSICSLAGVEYGADPKKDVSIRVI
                     TDHIRGVTFMISDGILPSNEGRGYVLRRLLRRAARHGRLLGVTDSFLSKIALKVIEVS
                     GDAYSELREKKDYILNIIDVEENRFYETLDQGLSILQGYIDELKLTNTDTLSGENAFR
                     LYDTYGFPLDLTKDILEEQGLSVDEETFQKEMQNQRERARAARADSSAESWKEDIYAK
                     IDWSIKTKFVGYESFSAKAIVKAIVRDNELEDSANEGDEISVVLDITPFYAESGGQVA
                     DTGRIIGEGFELDVKDCKKVTGERFAHVCTVISGEIKVGDSCIAEIDSKRRMATARNH
                     SATHLLHKALRKYLGTHVEQAGSYVTPERLRFDFTHFKAMTKEELDRIEREVNEAILA
                     SYEVITKETSINEAKKLGAMALFGEKYGEVVRVVKMGDYSIELCGGTHLKATSQAGFF
                     KIISEGGIAAGVRRIEAATGEKAYEFIKEEEKLMNELCGKLKAHPSECVKKMDTVLKQ
                     LKDTEKEVSVLKSKMAAEMLDELAAGAQEEKDIKYLVTKLDNMGIDSLREMGDKLRDK
                     LGTSFVMLASFDGEKTVLVAMASKDAVSKGVHAGNIIKEAAKAAGGSGGGRPDMAQGG
                     IKDLDKVAAALDAVPEILKNMVK"
     gene            complement(11913..12965)
                     /locus_tag="PKU88_11100"
     CDS             complement(11913..12965)
                     /locus_tag="PKU88_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPX69856.1"
                     /translation="MNNIWGSKKVNIRLIHIIIIAALVFIVLKYGIAISTVIKPFIIA
                     AAFSYLLNPIVKVFEKKGIRRIFGVLIVYLIFIAIIMLLSFVLVPKLIKEISTLVVNI
                     PQYSRQMQELFRQFQDGYMRSGLPESFKDVLDDNILMLQSMILTLLQNIANGIIEIFS
                     QFFNIVIVPVITFYMLKDAEYFKNQFILVLPKAKRAKLIILIRDIDNVFGKYIRGQII
                     IASFVGVLTTAALILIKVKYAFILGIFAGIANIIPYFGPFIGIIPTILFALLDSTGKA
                     LYAAGAFILIQQLESGFLTPRIIGKSVGIHPVYVIMSLIAGGRLFGIIGLIMAVPVLA
                     AIKLTLRHVFRDRQGS"
BASE COUNT         3351 a         3224 c         2411 g         3942 t
ORIGIN      
        1 tttcctccct gggccttctt actgacagca gtgtgttgta tttgtgcaaa tactcagtat
       61 tgataaacag gttgttaaag gcaggatgtg ccaagtcggc agactgatga gcaattgaaa
      121 cttccaggta gcttgttact tctaaagttc ttacctgctg gctgtgattt gtaagggtaa
      181 gccttctgac ttccgcatca tcctccggtg acacaattat ctcagtgtta gtgtcaatat
      241 cgtcatctgt cctggaaatg catatcttgt cctgagaaaa tactaccttg tacttgttgg
      301 gcagtacact gcaaggagcc agggaatttg accaaaccga accggaatcg atatcctgta
      361 agtagaagaa catgcctgaa tagtttaaaa cactgtcctc tctccatctg gtcactgcca
      421 aatccctgta tttgctgaac cccgaaccgt tgtccgttac cattacggaa taatccccat
      481 ttgacagcat gtgcacttcc ggaagtatat tgttgttcag tccgagaact ttggtatatt
      541 ctacgtcttc tttcacaact acttcaaagg gttcgatttt ttccttgtag tcctttgcga
      601 aaatcacctt tgagggtatc ttctcctgca tgagtatttc cgccgctttt atgacgggtt
      661 ccctgtggaa tctttcctgc agtatataat tgttgataac attattcagc gccataaaac
      721 tcataccctg atgatgagcc ataaagcttc tgactatgct gcttcttttt ccccttttta
      781 atctttcggg ggtataatct atagcttcat aaaagccata aggactatcc agcccttcct
      841 ccttcaaagt taagatattt ttgaaggact ctttcggatt caccatcaag gccagaaccg
      901 tagcataggg cgctattacc gtatcattaa tcaaccccct cttaagcccc aggcttggta
      961 tcccaaaagc cttatactga tagctcagcc tgaagtcaaa gctgtagtag ccggactccg
     1021 aaacccccca gggtactttc ctcttatttc cgtaatcctt ttggcatctt actacgaagt
     1081 catatgtctc atggagcagt gtgtccgaat agtccttcat aataagaagg ggcataagat
     1141 attcaaacat tgtacctgtc catgaaagca agcccttgaa gccgtcaacc tcagtcaatg
     1201 tcctgttgag catgaaccaa tgttccttct tgacctcacc cctggctatg gaaatcaggc
     1261 ttgcctgtct tgcttccgat gcaagaaggt cgtaatagga ctttgtaagc tgttcctcct
     1321 cctcattata gcctattgaa aacaattttc ttttattatc aaagagagga aggaaggacg
     1381 tttcttcaat aagcttgtca atccttccaa tcagccattc cgatttgaac atcagctcct
     1441 cattgttttc ctgcgaaatt ccgctgttta tttccttgag aaatgtgatt gcggacttat
     1501 ggagcttgat gccccagttg tatctcttca aatcattctt cccgataatt tttgtcagca
     1561 gctcaataaa accctgctgc tcaagcatgc ccccttccaa agccttgtca aaaatctcat
     1621 caggcagtct caattgttca tcctcaagct ttacaagctc cgaggtgtct ttaagtccca
     1681 ggaccatgcc cgaatcaacc gagggtttgt tcttatactc cagaagccct tgcctcaagg
     1741 tcatcaggta tccgataaaa ttgccgctgt ccactgttga aatatannnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnntcat tcatcctcct gtcatatttg gtcattacca
     1921 cttcagcaaa gctcacaaaa agtgggaaaa ctgcggctat taacgtgagc agcagccata
     1981 ccatggcatt tccgggcatt aaccataggg aaagaaagaa cagcagaacc agattgggcg
     2041 gaaccaggct tcttctcatg ttgtccagta ttttccacct gtttatcggt gaaagcggat
     2101 tcctgacaat ctctcccttt gcactgcgca cctttgaaaa aagccactgc agcagctgcc
     2161 aatcacccct tacccatctg tgaaggcgca ttgagctggc actatatttt gaagggtatc
     2221 cgtcaataag ctcaatatcc gtaacaagtc ctgttctgac atgggctcct tcaataaggt
     2281 catggcttaa tactgtgttc tcaggtatca tatttcttaa aatcccctga aaaactgcaa
     2341 gctcatatat ccctttacct gtaaatattc cttctccaaa gtaatcctga tatatgtcag
     2401 agacggcatt tgtataaatg tctatgcccc cctgtcccgc aaagactctt gcaaacctgg
     2461 aggtatttgc acttactatg tcgacactga tgcgtggctg cagcaatcca tagccttctt
     2521 ttacgacacc tctgccctca tccacaactg ctttgtttag aggatgagta attgtgccga
     2581 taagcttttt tgctgtttca atactgagtt ttgtgtctga atcaatagta ataacatact
     2641 ttacatcttt aagtgcgctg atatttccct cgatatgctc gaaggatgct tttttgcctc
     2701 ccagcagcag ttcattaaat tcataaaggg cgcctctctt gcgttcccag cccatccatc
     2761 tgttttgggt tttgctgaaa acccttcttc tatggaaaaa aaagaactta tcttcatcgc
     2821 ttccgtattt tctatttaac tcccttacct tctcgcttgc tgccagaata atctcctgat
     2881 ccccttcgga tttttccccg tcactgtcct tataatctcc aagcaaggca aaataaaggt
     2941 ttccctccct gtttgcaaga tagtattcct caagcttcag aagcagcttt acagcactgc
     3001 tgccctttgt tatcagtgta ggcatcacta caattgcagc tgcatcttcc ggtattccgt
     3061 ccttaagctc aagcttcggc agaaatgccg ggggctttat atgtacgagt atccaattga
     3121 ttgccgaaac cgaaacatca cttgccggaa ttaatactgc aagggctatc acaagctgtg
     3181 tccaaaaccc cgctgcacct tccctgtatg agtagttaag catcaaaaac actaagaacg
     3241 ccgttagagt aataattgaa aagaaataaa cccctgccgg ggaatagata tacatcccct
     3301 tgcgggcaat tctgtccagc aactgctcct tctgctccct gaacaggtaa tatccgatat
     3361 gcctgttatc ttccacagag cactctatta attttcttgc aacctttatc tccgatacct
     3421 taagcttttt ggaaagtatc tccaactcat ggcgatattt atcccttgaa gcaaagtcca
     3481 tcttctggta tatacctgcc ggatcctgcc tcaaaatttg ttcaacctgg ctgagttctt
     3541 caaaaatcac cttccaatcc aaagtggata tgaacctcag gctggtaatg gaattgccca
     3601 ttgaaacctg gcgggcagcc tgttccttgt gttccagctg ggtgatgctt tccgaggtgg
     3661 tatgctgctc ggacagcctt tcatcaatgt agtgtattat gggggctatt tctatgccgt
     3721 gctttttaag catattaagc aagtgttcgc cataggaggg gctgatattc tcaagctgcc
     3781 tcatgctttc cttggcaatg gcaagcagct cttccggctt tctgtcctta tatgaaagaa
     3841 tctgttcagc aagcttttca gctttatgcc actgctgctg cgataatgaa atgctttcac
     3901 agacatgtct tacatgttca ataagagcca ttctgatcat tatcggtata gcccagagtt
     3961 ccccgtttga aagcagtgat tgggactgat aagcggatac gaagcctacc agctgcttat
     4021 cgtcaaatct gccgtcagta tgggaaacca attccaaggc tatggcataa accctcggat
     4081 atcccttcag gaatcccgac ttcagcctgg gcagctggta ataatacttc ctggataagt
     4141 tctgccttat ttcctttacc tgttcctcaa taatataaaa attatccagc agccattctg
     4201 cagccggagt aacactgctt ctttcatttg catattcatt cagatcctta tagacggagg
     4261 taatgaaccg gaagttttta ttcatcctgc cctcaagact gaagtaatgt tttgccctct
     4321 tacccacaac atgcttgcgt gccaattcca atgcgtgttt ttccaattcc tccgcattaa
     4381 gtatcgcatc atatacttcc ggttcatctc tgtcaataaa gtataggagc ctgctgagca
     4441 agtatatcaa aacagcagca agaacaaatg ctgccatata aaaataatta gctaccaaat
     4501 tcaatataat cctcaccatt ccttcagttc atatttacgc tgcagaagaa gaatacggcg
     4561 gggacatggg gacgtttccc ctgtcccact ggaagtggga caggggaaac gtccccatgt
     4621 ccccgccgca ttcttctact tatcttcagc agagactatt ttttttattt tgtcggtgaa
     4681 ctcggctgca agctctcttg catactcctc ggatgctcct tccgcataaa cattgcacac
     4741 gggcctttcg ctgtcaggaa gtatgagaac ccatccgttt tcattattta tccttacccc
     4801 ttcaaagagt tcaatctctc tgttcttgtt ttcctcaatg atttctctta taacccttcc
     4861 cttatgctgc cagtcgcact ttacttcatt tttctccata tggaaatggg gaagctcatt
     4921 taccaggtca tacagtgtta cattattgcc tgccataaag tcaaccagat ttcctatacc
     4981 ccaaattccg tcaaagttaa gtatatattg gaacaaagat tcctccatcc cggtattttg
     5041 ctgaaacatc tctctcataa ttgccgcagg ggtattcttg gttctcacta cctctatgct
     5101 gtagcacttg gccatttcct ctattactct tgatgccgta tggggaatta taagcctttt
     5161 gtccattcct gtcttaagta ttattaatga agtcaaagca atctgcttat ccttatccag
     5221 tatcctcccc ttatggtcaa taagtacaaa actttctcca tcctcactga atactactcc
     5281 catacatgac ccgctcttga cagtcagctc ggctatatgt tcgtagtatg ctccggcact
     5341 gatattatcc ttggccggca tcttatacct gtcccaaacg ctatccctct ctactgaaca
     5401 acccatattt tttagataca tttgagctaa accggctatc tgtgaggata ttgaagctac
     5461 aaccagctta atcccggaac tcctcattct gtctgtatat ttcagcatgt ttatgccgct
     5521 ttggatatag aaatccgtga aattacccgc acttattatg tccttgattc tatctgcatt
     5581 gcaccttttg aaatcctccc tgataaagct attctcgatt tttctctcaa cggatctgtc
     5641 tatgcttatt cctttgctgc ctgagaattc aagatgcact ttgttcttat cactataatc
     5701 cttccatata tgaactccgc cttctgcctt gtagaatctg acggcgaacc tcattatagg
     5761 cactgctgcg ttttcaatgc ttattactcc tacacctgta gacagcactc cggatatcag
     5821 tgattccttc attatctcag cagagttcga accgtcgctg ctgacaatga ttccggaatc
     5881 atccttcagt attgagccga aagcggaacc aagccgcgaa gcaaattccg gcgtaaggtt
     5941 tatatttaca tccccgctga catccctgtg gccgaataaa gttcttgatg ctttcgtacc
     6001 ccatacaaga ttttgggcca ctacggcatc ttcctcaaca accttttcag gccatatctt
     6061 gatatccggc tttattatcg cgcctgtttc caaagtagta ttactgccta taacggtatt
     6121 ttcaaaaata tgcactccgc tttttactgc tgcaccgctg caaattactc ctcccctgca
     6181 ctggacattc tttcctatac ttgaattctt ccatattatg cttttcttta tggaggttgc
     6241 ttcctgtact tccgtgtcat tgcctaatat tgaggcttct attactgtcc tgtctttgat
     6301 tctgcagttg tctcctataa gcaccggtgc aataagatta cagctgtcgg aaatctcaca
     6361 gccgtcacct atccatattc cctccccatg ctgcctgtat ccgattgttg tcttgacctt
     6421 tcctgacaaa atgtcgaaat ttacttcctt gtatactctc aggtcaccga tgtcattcca
     6481 atagtcatcg gttacatatc cgtacatggg gatattatct cttaacagct ttggaaacaa
     6541 atccttgctg aagtcgaaat tatcaccctt actgtaatag tcaagcactt caggctcaag
     6601 tatatatata ccggtattaa tggtgtcgct gaacacctct ccccagctgg gcttttccat
     6661 aaagcttgtt attctggctt cttcgtcaat aataacaact ccgtattcca ggggcacggg
     6721 tacttttttc aatatcagcg tagctttcga tcttctgtat ttatgaaatt ccagtgcttt
     6781 ttcaaaatct atgtcagtca gggcatcgcc gctgacgata ataaaagtat catcgtagaa
     6841 atcctctgca ttttttacac ttccgccggt tcccaggggg gtatgctcaa tatagtacct
     6901 taaacttaca ccgaattctc cgccgtctcc gaaataatcg gtaatcactg cgggaaagta
     6961 tgccagtgtt gcggcaatat cagttatatt atgcttttta agcagctcta tggtatattc
     7021 cattaccggc ttattaagaa ccggcaccat tggctttggt attccgcagg tcaccggcct
     7081 gagtcttgta ccttctcccc cggccattat tacagccttc atcctgtcac ctcataacca
     7141 tacttttgct tatttataaa ttgcgctata caatattttc ccctataagc cggattttat
     7201 aacaacattt caatgagggc tttaaccggt tttaattaaa atttgaaaaa atagtgaaaa
     7261 tattatcaat tagtgttaaa atagtataga gctaaaaaaa acactgtaat ttctaatatt
     7321 atagtaataa taaacaaata tcaatgagaa agggtgtttt agagaaatga gtaaaccagt
     7381 attatccgct cactttgaat caaggacacc gtcaagtgtc cgactggccc agatgaaata
     7441 tgaggagcgg aaggtcaaac cccaggccgt aattaatgta ggtataggta atgtttcact
     7501 tcctaccaat cctgcaatgc aaaaaagaat gttttcgctt gatgcacctg aaagcccctt
     7561 tgcaaaaggt gtcataaggt atacaacaac tgccggcctt atagagtgtc aggacgcatt
     7621 taagaatatc ctcaaatgcg aaggcttcga cacaagcaag ctgtatgttc agataactga
     7681 cggcggatct gctgcaatgg aattactgtt attgggggtt tgcggaccgg caggtactga
     7741 tgaaaaacct cttatggtaa ttgacccggc ctataccaac tatatttctt ttgccgagag
     7801 agtcggacga aaaatagtta cggtaacccg ttcattaaat gaaaacggca agttcagcct
     7861 tccaaaattg aacgaaatcg aagaaactat taaaaagtat aatcccgggg cacttcttgt
     7921 tataccttac gacaatccga ccggccaatt ctatgattat gaaaccctta aagggcttgc
     7981 ggcattgtgc gtaaagtaca atatgtggtt tgtaagcgat gaagcgtacc gcgaactctt
     8041 ctatgaggaa ggcagcaagc ttgtaagcat ctggggagtt accgatgcgg atgtacccgg
     8101 tatcgaagga agaagaatga gcattgaaac tgcatcaaag gtatggaatg cctgcggact
     8161 gagaatcggt gccctgatta cagacagccc cgaattcaat aaccgttcag tggctgaata
     8221 tactgcaaat ctttgcgcta atgcaatcgg ccagtatata ttcggcgcac tggctcatga
     8281 aagcaaggag caaatagctg catggtgcaa gagtataaga gaatattaca aggaattgat
     8341 atttaatgtg tataacggac tgaagaaaca ggaaccgggc ttaatagttt caagccctga
     8401 tgcgtccatc tactcggtaa tcgatgtcag aaatgttgta aaaccgggat ttgatgcaac
     8461 tgagttcgta ctgtactgcg caggagaagg atcggtcaac ctggatgggg ttgagacaac
     8521 tcttctggta gctccaatga aaggattcta taatactaaa aaaggagaat acaacccggg
     8581 gagcacacaa ttccgtattt ccttcgtaga aacaccggat aaaatggcaa aggttccgga
     8641 gttgtttgcc aagcttctga gacagtacga agcacaaaga tagaagtcaa taaataaagg
     8701 gtgggaagca agctttccac ccttttagtt tgcagatgat atgtggggac acggggacat
     8761 ttcgggacac ggggacgttt cgggacacgg ggacgtttct cctgtcccac ttcagtggga
     8821 caggagaaac gtccccgtgt cccgaaacgt ccccgtgtcc cgaaatgtcc ccgtgtcccg
     8881 aaacgtcccc atgtccccct gcctcttgtt attttactac ttcaccatgt ttttcagtat
     8941 ctccggcact gcgtccaaag ctgctgcgac tttatccaga tcctttattc ccccctgtgc
     9001 catgtccggc cttccgccgc cgcttcctcc tgcagccttt gcagcctcct taatgatatt
     9061 ccctgcgtgt actcccttgg aaaccgcatc ctttgatgcc attgcaacca ggactgtctt
     9121 ttccccgtca aaggaggcaa gcatcacaaa ggaggttccc agcttatccc ttagtttgtc
     9181 tcccatttct ctcaggctgt caatacccat gttgtcaagc tttgttacaa gatattttat
     9241 gtccttctcc tcttgtgccc cggctgcaag ctcatccagc atttccgccg ccatttttga
     9301 tttcagcaca gatacttcct tttccgtatc cttaagctgc ttgagtactg tatccatttt
     9361 cttgacacat tcgcttggat gggccttgag tttcccgcac agctcattca tcagtttttc
     9421 ttcttcctta ataaattcat aagctttttc tcctgttgcc gcttcaatcc ttctgactcc
     9481 ggcagctatg cccccttcgc ttattatttt gaagaagccc gcctggcttg tggctttcaa
     9541 gtgtgttccc ccgcagagtt caatggaata gtcgcccatt ttgacaactc ttaccacttc
     9601 accgtatttc tctccgaaaa gcgccatagc tccaagcttt ttcgcctcat ttatggaggt
     9661 ttcctttgta attacttcat agcttgccag aattgcttca tttacttctc tttctatcct
     9721 gtcaagctct tcctttgtca tcgccttgaa gtgagtgaag tcaaacctta aacgctccgg
     9781 cgtaacataa gagcctgcct gctcaacatg agtgccaagg tattttctca atgccttatg
     9841 aagcagatga gtggccgagt ggttcctggc ggtagccatt ctcctttttg aatcaatttc
     9901 tgctatacag gagtctccga ccttaatttc accgctgatt accgtacaca catgggcaaa
     9961 cctttccccg gtgaccttct tacagtcttt tacatccaac tcgaagcctt ccccgattat
    10021 tcttccggta tcggctacct gtccgccgct ttccgcatag aaaggtgtaa tatcaagtac
    10081 tacgcttatt tcatcccctt cattggctga gtcttccagt tcattatccc ttactattgc
    10141 tttgacaatc gcttttgcgg aaaagctttc atatcccaca aacttagtct ttatgctcca
    10201 gtcgatcttt gcatatatat cttccttcca gctttcagcg ctgctgtcag cccttgctgc
    10261 ccttgccctt tccctttggt tctgcatctc cttctggaag gtttcctcat caacagatag
    10321 tccctgctcc tccagtatgt ctttagtcag atccaggggg aagccgtagg tatcataaag
    10381 cctgaaggca ttttctcctg acaatgtgtc tgtatttgtc agcttcaact cgtcaatata
    10441 accctgaagt attgaaagtc cctggtccaa tgtctcataa aacctatttt cttcaacatc
    10501 aattatattt aggatataat ccttcttttc cctcaattcg ctgtatgcgt ctccgcttac
    10561 ttcgataacc ttcagggcaa ttttgctgag gaaagaatcg gtcacaccca gtagccttcc
    10621 atgccttgca gctcttctta gaagccttct aagaacatat cctctgcctt cattgcttgg
    10681 aagtatgccg tcggatatca tgaaggtaac tcctctgata tgatcggtaa taacccttat
    10741 tgaaacatcc ttcttcggat ccgcaccgta ttccacccct gcaaggctgc agatgctgtc
    10801 caatatatac tttatagtgt ctacctcaaa tatgttgtca actccctgca taaccgcagc
    10861 cattcgctcc aatcccatgc ctgtgtctat atttggcttt gccagtctgt tgtaattgcc
    10921 ttcttcgtcc ttatcaaact gtgtgaatac aaggttccag aattccatat acctgtcgca
    10981 gtcgcagccc agcttgcagt cttccttacc gcagcctgta cccggccctc tgtcaaaata
    11041 tatctctgaa caaggtccgc aggggccaag gccgatttcc cagaagttgt cctccttacc
    11101 cattctaaca atcctgtccg gcggcaatcc tatcttgtta tgccagatat caaaggcttc
    11161 atcatcgtcc agataaatag taacccaaag cctgtcttcc ggcatcttat atacctcagt
    11221 gcacagctcc catgcccagg ctattgcctc atccttgaag taatctccga aggagaaatt
    11281 acccagcatt tcaaaaaagg ttccgtgtct ggcagtcttt ccaacccttt caatatcagg
    11341 ggtcctgatg cacttctgac aggttgtcac ccttaagctc ggaggtacgg cctgcccggt
    11401 aaaaaacggc ttcaaggggg ccatacccga gtttatgatg agcaggctct tgtcactctg
    11461 tggcacaaga gatgcacttt tcattcttaa atgatccttg ctttcaaaaa agctaaggaa
    11521 tttttctctt acttcattaa gtcctaactt ttccatgtgt attcctcccg tatatttttg
    11581 taaaaaaaac tccccatccc ggttagggac gaagagaact tcgcggtacc accctagttg
    11641 cccgaaaggg ccgctttgtt ggctttagcc atgcagtata acgcctgcag cgtctttacg
    11701 atgctcagca gcagcttcaa cagcttattc cctgggattc tcaccagcca tcccttctct
    11761 gcttatgctg ccatattttg cagcatattg aaaatcagtc tgtttactcc tctgcatcaa
    11821 cacaatatta aatatatacg aagattatat attagccggg aagctttgtc aataattcac
    11881 attaaaactc aaattcaacc attaagcagt ccttaactcc cctgcctatc tctgaataca
    11941 tgtctgagtg tcagcttaat tgcagccaat acgggcacag ccataataag ccctatgatt
    12001 ccaaaaagcc ggcctcccgc tataagggac attataacat aaacagggtg tattcccaca
    12061 ctctttccta ttatccttgg tgtaaggaat ccgctttcca gctgctgtat cagaataaag
    12121 gctcctgccg catacaatgc tttaccggtt gaatccagca atgcaaacaa tattgtaggt
    12181 ataattccta taaagggccc gaagtatggt attatatttg ctataccggc aaagatgcca
    12241 agtatgaagg catattttac tttaatcagt ataagggcag cagttgtcag tacacccaca
    12301 aagctggcta taataatctg ccctcttata tatttaccaa aaacattatc aatatccctt
    12361 attaatataa taagtttcgc gcgctttgcc ttcggcagta caagaatgaa ttggttcttg
    12421 aagtactccg catctttcag catataaaaa gtaatcaccg gaactattac tatattaaaa
    12481 aactgtgaaa agatttctat tatgccgttg gcaatgttct gcaaaagtgt gagaatcatg
    12541 ctttgcagca taagtatgtt atcatccagt acatccttaa aactctccgg aagcccgctt
    12601 ctcatgtatc cgtcctggaa ttgccgaaaa agctcctgca tctgtcttga gtattggggg
    12661 atgttgacaa ccagtgtgct gatttccttt atcagcttag gaacaagcac aaaagacagg
    12721 agcataatta tagctataaa aatcagatat acaataagaa ctccgaaaat ccttctgatg
    12781 cctttttttt caaaaacctt cactataggg ttcagcaggt aagagaaggc tgccgcaatt
    12841 ataaagggct ttatcactgt gctgatggca atgccatatt tgagcacaat aaaaaccaat
    12901 gctgcaatta tgataatatg aataagtctt atattgacct tctttgatcc ccaaatattg
    12961 ttcatactgt tcatcctata taaatattgt ggcagaattg aagaagaatg cggcggggac
    13021 acggggac
//
LOCUS       DAOOAB010000063        12387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_6558_length_12387_cov_16.772867, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000063 DAOOAB010000000
VERSION     DAOOAB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6558_length_12387_cov_16.772867"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1156
                     /locus_tag="PKU88_11105"
     CDS             116..1156
                     /locus_tag="PKU88_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPX69857.1"
                     /translation="MSEKSNVAENQLHYQEVLPKTINGMLMLVVNIILYIASIALFIF
                     AVNLIDSGKNIILGVILIIVCVIYAFVAGPLLFIGLKILKPNEALVLTLFGKYYGTLK
                     GAGFFFVNPFAAAANNATHAAATAVLSADISPLAGKTAGKAADAVFRNKKIISLKAMT
                     LSNDKQKINDQLGNPIIIGIVVIWKVVNTAKAVFNVDNYIEYLSIQCDSALRNIVRLY
                     PYDASSEDNEKSLRGSSQEIADKIREEIQRKVEIAGLEIIEARITHLAYAPEIAAAML
                     QRQQASAIIDARQMIVEGAVGMVEMALAKLSENDIVQLDEERKAAMVSNLLVVLCGNK
                     DAQPIVNSGSLY"
     gene            1197..1376
                     /locus_tag="PKU88_11110"
     CDS             1197..1376
                     /locus_tag="PKU88_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPX69858.1"
                     /translation="MDNKEKEKKQIPLRLSSSLWKELAEWAEDDFRSINGQIEFLLTE
                     CVKQRKKRNISKPQE"
     gene            complement(1378..2640)
                     /locus_tag="PKU88_11115"
     CDS             complement(1378..2640)
                     /locus_tag="PKU88_11115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPX69859.1"
                     /translation="MIRSAKRYGKLALFLGLLNIAWVVNTVIFSYILEIPQIAPYIVS
                     QIILLLNAIGLIQFFLKEQRNEIERRSDQIAYLTFHDELTGLHNRAYFEKKIQELSKD
                     NDCLPVSLLIGDMNGLKFINDVFGHQKGDKWLKRIAAIIQQSCRQNDIIARWGGDEFA
                     VIMPNTDREKALDIAHEINKACKRNQEEDSFFSISLGVATKNDSGTDLSKVLTEAEEL
                     MYQNKLIEGKKTRLAFVEVLGNLLHKKGYEPKEHIERLAALAKEFAAVLNLSRENRKY
                     LVQTANLHDIGKIGIPEDIVLKESRLDESEWPIMKKHVEIGYRLAQISGEFAHLADVI
                     LYHHEWWNGQGYPHGLKGEDIPLLSRIISILHAFDVMTHQQPYKPAGTIDEALHELSL
                     NAGTQFDPALVPVFIDMISDIALDKDCN"
     gene            complement(2960..3042)
                     /locus_tag="PKU88_11120"
     tRNA            complement(2960..3042)
                     /locus_tag="PKU88_11120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3006..3008),aa:Leu,seq:gag)
     gene            complement(3106..4704)
                     /locus_tag="PKU88_11125"
     CDS             complement(3106..4704)
                     /locus_tag="PKU88_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX69860.1"
                     /translation="MKDKRFLLIFILLMLLSLVSCEESVVPKETEIAVENEEPVEGGT
                     INISSVEPGSLNPLLNKSKSYNEISKLIFQSLVEYDANLKIIPVLAQSWDFMDGSNKF
                     VIKLKENVFWSDGESFTADDVKFSLDTIKASEDSIYKGNLEHIYSYKVKDASTIEITF
                     DQPFVTAIDMLSFPIIPRHIYKSNPNAVPIGTGMFKVSEYNRLKSMELVYNDKWNGEE
                     KPYIEKIRITFINDVDAFSIAFQSKELDLLNTTSYDWEKYSEMKDINAYKYTTMYYDF
                     IGLNYNNPIFQDKAVRKAMIQGINRKSIVDKYLLGNAAVTDVPINPTSWLKDADAEKY
                     GYSRSEAQNLLMAAGFADLNNDGILERKVAEAVQQLSFTLLTNDENEFRRKAAEEIKK
                     NLEDIGFRVEIKFAGFDDVKAAIETGEFDAVHTGYNLSNTQDLSFAFHSTQIDSGKNF
                     MAYSNPEIDALLLQAYTAMDDNRRKEIYKNLQKSFREEVPCISLFFREAALVVRDKIQ
                     GEIKPDTVNPYRSIHKWFVPESKR"
     gene            complement(4760..5317)
                     /locus_tag="PKU88_11130"
     CDS             complement(4760..5317)
                     /locus_tag="PKU88_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPX69861.1"
                     /translation="MAKVRKLKRIRLLVAVLLIYAAVLNIPNLLKLLYPIEHKEIIIK
                     YGQMHNVDPLLLAALIKTESNFEPRAESRKGAKGLMQITPSTGEWIAKTIGVNDFNED
                     MLFDPETNIMLGSWYIEHLTNYYNGIFELVFAAYNGGRGNVDKWLKDKNLSSDGITLD
                     TIPFSETKNYLEKLRKNYNIYKIIY"
     gene            complement(5321..5917)
                     /gene="coaE"
                     /locus_tag="PKU88_11135"
     CDS             complement(5321..5917)
                     /gene="coaE"
                     /locus_tag="PKU88_11135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907570.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPX69862.1"
                     /translation="MVIGLTGGIASGKSTVSAKLKELGAAVIDADLLARDVVRKGEIA
                     YNKIVQCFGADILLPGGDIDRKKLGDIVFSDKEKLELLNSITHPEIINRMKERIQELK
                     AEGAKVIVVDAAILIEMGLHKYVDSVWVLSVDRETQLKRLAERDKYDYREAENRINSQ
                     FTDEVRKKYADVVIDNSKPIEEVEKRLEELWNNIAGGE"
     gene            complement(5936..8545)
                     /gene="polA"
                     /locus_tag="PKU88_11140"
     CDS             complement(5936..8545)
                     /gene="polA"
                     /locus_tag="PKU88_11140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPX69863.1"
                     /translation="MEKGKFIIIDGNSLMHRAFYALPNLTNSKGFHTSVIYGFVNMIN
                     KIKEEYKPQYIGIAFDTKAPTFRHQEYAEYKAGRLKMAEEMAEQIPVLKEVLAAMNIR
                     QVEIDGFEADDIIGTVAARCDGEGIPTFIVTGDKDAFQLISDNVHTLMTKKGISEIEE
                     YDTAKLHEKYGIAPAQITDLKGLMGDASDNIPGVPGVGEKTALELIHQFGTVENTLEN
                     ASEIKKNKVRENVSSNMDQAILSKRLATIVKDVPIDFELGDFALKEADKEKLYSLFAE
                     LEFKGLIEKMCGSNSCTEAAGTVEIAVKEIDGIDELKKVFEEVSKCGKFAFKIDDELG
                     EMLRTIYINADGKQYRFNINDSNMPLVKEIMEDSGVLKTGHDIKQDILLLRRYGIDVG
                     PIGFDTMIAAYLLNPSKGSYRIKDLSQEYLGYGVDGYDAKGAEQVQKYAATVKAAAEL
                     QPVLTGRLEEYGMIELYNSVELPLVKVLADMEHEGFSVDKAMLEVLSGDFAKQIDALT
                     SEVYRLSGEEFNINSTRQLGTVLFDKLGLPAMKKTKTGYSTDVEVLEQLYDKHPIIQK
                     LLEYRQLVKLKSTYVDGLIGIINPETGRIHTKLNQTVTVTGRLSSTEPNLQNIPIRTE
                     NGRQIRKVFVPRSQDYILVDADYSQIELRVLAHISGDKGLIDTFKRNEDIHTRTASEV
                     FGIEIKDVTPLMRGRAKAVNFGIVYGMSDYGLSQDLKISRKEAKKYIDNYFARYPGVK
                     QYMTDIVEAARTDGYVKTIMNRRRYIPEITSRNAVNRNFGERIALNTPIQGSAADLIK
                     MAMVKVYDELKKRNLKSRLILQVHDELILETHKDELSEVKNLVKECMENAIKLSVPVI
                     AEVGSGDSWYNAK"
     gene            complement(8685..10034)
                     /locus_tag="PKU88_11145"
     CDS             complement(8685..10034)
                     /locus_tag="PKU88_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HPX69864.1"
                     /translation="MKCETIKNMMSSYIDKDLNNIEKAEFEKHLAECEECRAEYESLT
                     DIIAACGNLEEIELPQNFRTELHQRLNEEKSKRNFFSGIMGSKRMKMAAGLVAAALVI
                     AIGIGGSSLLFDNSMKMAQSADSAPGYGIAPAPAAMPEAPAAPPVYYDSDLAENKAKS
                     REFETAAAEERGMGEDSVGMQFNESLMAKEMAPSQGLEGQAAESARSGRMVIRSGNIS
                     VNVENVDKAAADIRQMTESSGGYVENSQIDNVTVPQFPVYDAGSEARETTQKYANMTV
                     RVPENRFEDIFNNIKGMGKLVSENISGSDITAEYRDTTARVDNLKIQEQSLQQLMAKA
                     KTVDEILKIESELNRVRTDIDINSGNLKRWDNLVQLSTIYVYMRELKPEELKSVDVPG
                     TWEKAYQGFIKAVNNVVEGFQKTLIVLVAAIPYLFVAGVIAAIGFLAARKIKLKKKQ"
     gene            complement(10060..10653)
                     /locus_tag="PKU88_11150"
     CDS             complement(10060..10653)
                     /locus_tag="PKU88_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPX69865.1"
                     /translation="MSDLEKLLIKKSQSGDVESFELLISSYDKRAYNIAYRIMGNEED
                     AKDMAQEALLRVFKSLKDFKGQAAFSTWLYRIVTNVCLDELRRRKNEKYVSMDSSIHT
                     EDGELHMELCSDKETPESVYERIEQRELIRNAIREMNEDYRTVIVLRDIQGFSYDEIS
                     GMLDCSLGTVKSRINRARTMLRDKLKESMELSRENIV"
     gene            complement(10907..12289)
                     /locus_tag="PKU88_11155"
     CDS             complement(10907..12289)
                     /locus_tag="PKU88_11155"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPX69866.1"
                     /translation="MKRSFFIALIVIICFSRVLADSGSNKSAIEINLPSGTLSLYKDG
                     NLAKEYPLCLGKQSTPTPAGQYRIIYKAVNPYWLNKGDVVPPGPQNPLGVRWMGITRG
                     IGIHGNNKPESIGTYASAGCIRMFNRDVVEVYNQVTVNTPVNIKYDRLKLFEDKYSMK
                     KSIIIYPDIYKKGAEIARQQLAQLSNTDIPEEILKKAREIIAKPVAKPMAVSEGIGVF
                     LNNSLITCDALEEEGKIYVNYKAAEDILGLTPELAGKFNIGIKELEGVIYINLNDTAD
                     SFGGTVSYDEGAGNAYMDMEVVKVNGVFAGINHGDYDKSDYLTVEAVKNLGYEYSEDS
                     VDIRIFGKGIMKLKRNNAWVINADNLAEALGGSKDANSRYRIVDLKLPVYLKVGKEYF
                     KTENIEGELVLNAETAYSIYERSGRAVETFSAQGQNIDEKIDLETFLEGHDYTVNDFY
                     TLIDIKLKEN"
BASE COUNT         3260 a         2982 c         2131 g         4014 t
ORIGIN      
        1 aacaaataaa attagcgcat cttgatgcaa aaattgcttg acattttagt aaccttggta
       61 taatataaat ttgatatcat attgatatca tatttatatg aggaggttaa ttaaaatgtc
      121 cgaaaaatca aacgttgctg aaaaccaatt gcattatcag gaggtattgc ctaaaaccat
      181 taacggcatg ctgatgcttg ttgtaaacat aatactttat attgcttcta ttgctttgtt
      241 tatttttgcc gtaaacctga tcgatagcgg gaaaaatatt attttaggag tgatactaat
      301 aatagtctgt gttatttatg catttgtggc tggaccccta ttattcatcg gtttaaaaat
      361 tcttaaacct aatgaagctc ttgttctgac gcttttcggt aaatattacg gtactctcaa
      421 gggtgccggg ttcttctttg tcaatccttt tgcagcagct gccaacaatg ctacccatgc
      481 agctgctaca gccgttttat ccgccgatat ttcaccactt gccggtaaaa ctgcgggtaa
      541 ggcagcagat gccgttttca gaaacaaaaa gatcatttcc ctgaaagcaa tgaccttgag
      601 taacgacaag caaaaaatca atgaccagct tggaaatccc ataatcattg gaattgttgt
      661 catatggaag gtagttaata ccgcaaaggc ggttttcaat gtggataact acattgaata
      721 tctttccata caatgtgact cggcacttag aaatattgta agattgtatc cctatgatgc
      781 ttcaagcgaa gataatgaaa aatctcttcg aggaagcagc caggaaatag cggataaaat
      841 aagggaagaa attcaaagaa aagtggaaat cgcgggcctt gaaattatag aagcaagaat
      901 cacacatttg gcttatgcac cggaaatagc cgctgcaatg cttcaaaggc agcaggcttc
      961 cgcaatcatt gacgcaaggc agatgattgt tgagggagct gttggaatgg tagaaatggc
     1021 tcttgcgaag ctgagcgaaa atgacatagt tcagcttgat gaagagcgca aagcagcaat
     1081 ggtaagcaat ctgctggtcg tcctctgcgg taacaaagat gctcaaccca ttgtcaacag
     1141 cggatcatta tactaatata tcattatgct aatatataga atggagggat gatgatatgg
     1201 ataacaaaga aaaagaaaaa aagcaaatcc ccctgagatt atcatcatct ctttggaagg
     1261 aactggcgga atgggccgag gatgacttcc gctccatcaa tgggcagata gagtttttat
     1321 tgaccgaatg tgtgaaacag cgtaaaaaaa gaaatatctc caagcctcag gaataaatca
     1381 gttgcagtct ttatccaaag ctatatcaga tatcatatca ataaaaacag gaactaaagc
     1441 gggatcaaac tgggttccgg cattcaggga aagttcatgc agagcttcgt ctatagtccc
     1501 ggcaggtttg taaggctgct gatgggtcat aacatcaaaa gcatgcagaa tggatatgat
     1561 tcgcgacaaa aggggaatat cttccccttt cagtccatga ggatagccct ggccgttcca
     1621 ccattcatgg tgatatagaa ttacatcggc taaatgggca aattctccgg aaatctgggc
     1681 taatcggtat ccgatttcaa catgtttttt cataataggc cattctgatt catcaagccg
     1741 ggactctttc agaactatat cttcaggtat gccgattttg cctatgtcat gcaaatttgc
     1801 agtctgcacg aggtatttac ggttttccct tgaaaggtta agaactgccg caaattcttt
     1861 agccaaagct gccagtcttt caatatgctc tttgggttca tagccttttt tatgcagcag
     1921 attgcctaat acttcaacga aagccaacct tgttttctta ccttctataa gtttgttttg
     1981 gtacattaat tcttcagcct cagttaagac ctttgacaaa tctgtcccgc tgtcattttt
     2041 agttgccaca cccagggata tactgaaaaa agaatcctcc tcctgatttc ttttacaggc
     2101 cttatttatt tcatgtgcga tatcgagagc cttttctctg tccgtgtttg gcataataac
     2161 agcaaactca tcgccgcccc acctagcaat aatatcattt tgacggcagg attgctgaat
     2221 aatggcagct atccttttaa gccatttgtc tcccttctga tggccaaaaa catcattaat
     2281 gaatttcaat ccgttcatat ctccaatgag caaagagacg ggcaggcagt cattgtcctt
     2341 tgacagctct tgtattttct tttcaaaata agccctatta tgcaaaccgg ttaactcgtc
     2401 atggaatgta aggtatgcaa tctgatctga tcgccgctca atctcatttc tttgctcctt
     2461 taaaaaaaac tgaataagcc ctattgcgtt taaaagtaag attatttgtg aaacaatata
     2521 gggggcaatc tggggtatct ccaaaatata tgaaaaaata accgtattga caacccaggc
     2581 tatatttaac aaacctagaa aaagagcaag ctttccgtac cttttggctg acctgatcat
     2641 agctgtgcct acccaggcgg acaaagcaaa taccgataaa taggtatgtt taatcatctg
     2701 cagatccgac caattccggg ctgagaaaaa gatgaaaaat aatagccaaa ccatccctat
     2761 gctcaaaaac tgggatgttt tgactttagt tttcaggaat attaaagtac cctgtgatat
     2821 taaaaggttt gcataaaaat atgaaatcag atatgaattg ccaagaaatc tgcatattat
     2881 agtggtgata ggcttgtctg tttaaagtgc aaaaaataaa aaacttcaaa gctcatagcc
     2941 ttaaagtcaa tattcattat ggtgcggtcg atgggacttg aacccatacg gtcacccaca
     3001 cgcccctcaa acgtgcgcgt ctgccagttc cgccacgacc gcgtgtcaca tagattatta
     3061 tataacctaa tggcttatgt gtcaatagga ttgtaatggt tctttctacc tcttactttc
     3121 cggcacgaac catttatgta tgcttctata tggattcacc gtgtcaggtt ttatttctcc
     3181 ctgtattttg tcccgcacaa ccaaagcggc ttccctgaaa aaaaggctta tgcacggaac
     3241 ctcttccctg aagcttttct gcaggttctt gtatatttcc ttccgcctgt tgtcatccat
     3301 tgcagtatat gcttgcagga gaagcgcatc aatttcgggg tttgaatagg ccatgaaatt
     3361 ttttccgctg tctatttgtg tgctgtggaa tgcaaaggat aagtcctgcg tatttgacag
     3421 gttgtagcct gtgtgtactg catcaaattc accggtttct atggcggctt ttacatcgtc
     3481 aaatccggca aattttattt cgacacgaaa tccgatgtcc tccaggtttt tctttatttc
     3541 ctcagcggct tttctcctga attcattctc gtcattggtc aaaagcgtga agctcaactg
     3601 ctgtactgct tctgcaactt ttctctccaa tatgccgtca ttatttaaat ctgcaaagcc
     3661 tgcagccatt aaaagatttt gagcttcaga tctgctgtat ccgtattttt cggcatctgc
     3721 atccttcaac caggaagttg gattaatcgg cacgtctgta actgctgcat tcccaagcaa
     3781 atatttatca acaattgact ttctgttaat accttgaatc attgcctttc ttactgcttt
     3841 atcctgaaat attggattat tgtaattcag tccgataaaa tcataataca tggttgtata
     3901 tttataggca ttgatatctt tcatttcgct gtacttttcc caatcatagg aggttgtgtt
     3961 caacaggtcc agttccttag actgaaaagc tatggaaaac gcatcaacat catttatgaa
     4021 agttattctg attttctcta tatatggctt ttcttcgcca ttccacttgt cattgtatac
     4081 cagttccatg gatttgagcc tgttgtactc cgaaaccttg aacatacccg tccctatcgg
     4141 aacagcattc ggattgcttt tataaatatg ccggggtatt atcggaaaac tcagcatatc
     4201 aatcgcagtt acaaagggct ggtcgaaggt tatttctatt gttgaggcat cttttacttt
     4261 ataagaataa atatgctcca aattgccctt atatatagaa tcttcagatg ctttaatcgt
     4321 atccaaagaa aattttacat catccgcagt gaagctttct ccgtcgctcc aaaacacatt
     4381 ttctttcagc tttatcacga acttgttgga gccatccata aaatcccagc tctgtgcaag
     4441 aacaggaata attttcaaat ttgcatcata ttcaaccagg ctttgaaaaa tcaacttcga
     4501 tatctcatta taggacttgc ttttgttgag cagcggattc aatgaccccg gttccaccga
     4561 gcttatattt attgttccgc cttccacagg ttcctcattt tctacggcaa tttccgtttc
     4621 cttcggcaca actgattctt cgcaggacac caatgaaagc agcattagca atataaaaat
     4681 caataaaaac cttttatcct tcattacttg ttctccatcc tttatcaaac cgtttatgtt
     4741 gcggatcggc agcatcccgt taataaataa tcttataaat attataattc tttcttagtt
     4801 tttcaagata attttttgtc tcggaaaacg gaatggtatc cagtgtaatg ccgtcgctgc
     4861 tcaaattctt gtctttcagc cacttgtcca catttccgcg tcccccgtta taagcggcaa
     4921 aaaccaattc gaagatgcca ttgtagtaat ttgtaagatg ctcaatatac cagctgccaa
     4981 gcataatatt ggtctcaggg tcaaaaagca tatcctcgtt aaaatcattt acacctatgg
     5041 tttttgctat ccactctcct gtcgaaggtg taatctgcat aagccccttt gccccttttc
     5101 tcgattctgc ccggggttca aagttgcttt ccgtctttat cagtgcggca agcagcaaag
     5161 gatctacatt gtgcatttgt ccgtatttta ttattatctc tttatgctct attggatata
     5221 aaagctttag taaattcggt atgttaagta ccgcagcata tatgagcaat actgccacta
     5281 atagcctgat acgctttaat ttcctgactt tagccaaata ctattctccc ccagcaatat
     5341 tattccataa ttcttccagc cttttttcga cttcctcaat gggtttgctg ttgtcaatta
     5401 ccacatccgc atattttttt cttacttcgt cggtaaattg tgagtttatc ctgttttctg
     5461 cttctctgta atcatattta tcccgttctg caagcctttt cagctgggtt tccctgtcta
     5521 ccgacaaaac ccagacactg tccacatatt tgtgcaatcc catctcaatc agtatggcag
     5581 catccactac tattaccttg gctccttccg ccttaagctc ttggattctt tccttcattc
     5641 tgtttattat ctcaggatgg gttatgctat tcagcagttc cagcttttct ttatccgaaa
     5701 aaacaatatc ccccagcttc ttcctgtcga tatccccgcc gggcagcaat atatccgctc
     5761 caaagcactg aactatttta ttatatgcta tttccccttt tcttacaaca tccctcgcaa
     5821 gcaaatccgc atcaataaca gccgctccca attctttaag cttggctgaa acagtactct
     5881 tgccgcttgc tattcctccc gtcaaaccaa taaccacagc acatcccacc cattcctatt
     5941 tagcattgta ccaactatct ccgctgccga cctcagcaat tacaggcaca cttaatttga
     6001 tagcgttctc catgcattcc ttcaccagat tcttcacttc cgaaagttca tctttgtgag
     6061 tttctaaaat aagctcgtca tgtacctgca gtatcagcct tgactttagg tttctctttt
     6121 ttaattcgtc atacacctta accatggcca tttttatcag atcagccgcg ctgccctgaa
     6181 taggagtatt caaggctatt ctttctccaa agttcctgtt caccgcattc ctggaggtta
     6241 tttccggaat atatctcctt ctgttcatta tggtctttac atatccgtct gtccttgcag
     6301 cttcaacaat atcagtcata tattgcttta ctccgggata tctggcaaag taattatcta
     6361 tatatttttt tgcttctttt cttgatatct taagatcctg tgacaggcca taatcactca
     6421 ttccgtatac aataccgaaa ttcaccgcct ttgcccttcc cctcataagg ggtgtcacat
     6481 ccttaatctc aattccgaat acctcggaag cagtccttgt atgtatatct tcatttcgtt
     6541 taaaggtgtc aataaggccc ttatctccgg atatgtgcgc caataccctc agctcaatct
     6601 gtgaatagtc ggcatctacc agaatataat cctggctcct cggtacaaaa acctttctta
     6661 tctgtcttcc attttcggtt cttatgggta tgttctgcaa attgggctct gtgctgctga
     6721 gccttcccgt aactgtaacc gtttggttca gcttggtatg tatcctgcct gtttcgggat
     6781 ttattatgcc aatgaggccg tctacataag tggatttaag ctttacaagc tgcctgtact
     6841 caagtagctt ctgaattatc ggatgcttgt cataaagctg ctccaatacc tctacatctg
     6901 tagaatagcc ggtcttggtc ttcttcatcg caggcagccc cagtttatca aacaatactg
     6961 ttccaagctg cctggtggaa ttaatattga actcttctcc ggacagtctg tatacctctg
     7021 atgtaagagc atctatctgc ttcgcaaaat caccggacag cacctccagc atggctttat
     7081 ccacactgaa gccttcatgc tccatatccg caagtacctt taccaggggc agttcgacgc
     7141 tgttatacaa ttctatcatt ccatattctt caagtcttcc tgtaagtacc ggctgcagct
     7201 ccgccgccgc ttttacagta gctgcgtact tttgaacctg ctccgctccc ttagcatcat
     7261 acccgtccac accatagccc agatactcct ggctcaggtc ttttatccta taggagccct
     7321 ttgagggatt aagcaggtat gcggctatca tggtatcaaa gccaataggg ccgacatcta
     7381 ttccatacct tctaagcagc aatatatcct gttttatatc gtggcccgtc tttaataccc
     7441 cggaatcttc cattatctcc ttaacaaggg gcatgttact gtcattaatg ttaaaacggt
     7501 actgtttccc atctgcatta atatatatgg ttctgagcat ttctcccagt tcatcatcta
     7561 tcttgaatgc aaattttccg cacttggata cttcttcaaa taccttcttc agctcatcaa
     7621 taccgtctat ttcctttaca gcaatttcaa cagtccctgc tgcttcagtg caggaattgc
     7681 tgccgcacat cttttcaatc aaccccttga actccagttc cgcaaaaagg ctgtacagct
     7741 tctccttatc cgcttccttc aaagcaaagt ccccaagctc aaaatcaatg ggaacatctt
     7801 ttactatggt agccagtctc ttggaaagga ttgcctgatc catattagac gacacatttt
     7861 cccttacctt gttcttcttg atctccgatg cattctcaag ggtattctct accgttccga
     7921 actggtgtat aagctcaaga gctgtttttt cccctacccc gggcactccg gggatattat
     7981 ccgaagcatc tcccataagc cctttaagat cagttatctg agccggagca attccatatt
     8041 tctcatgcag ctttgctgtg tcatattctt ctatttcgct tattcctttt ttggtcatca
     8101 gtgtatgcac attatcgctt ataagctgga atgcatcctt gtctccggtt actataaagg
     8161 tgggtatgcc ttctccatca catctggctg caaccgttcc gattatatca tccgcttcaa
     8221 aaccgtctat ttccacctgt ctgatattca tagcggccag aacctctttc agcacaggaa
     8281 tctgttccgc catttcctca gccatcttaa gcctccctgc tttatactcg gcatactcct
     8341 gatgcctgaa agttggtgcc ttggtgtcaa aagcaatgcc tatatattga ggcttgtatt
     8401 cttcttttat cttgtttatc atatttacaa aaccatatat tacacttgta tgaaagccct
     8461 tggaattagt gaggttgggc agtgcataaa aagctctgtg catcaggctg ttgccgtcaa
     8521 tgataatgaa tttgcctttt tccattcctt aaatcctccg tgagttattt gcatatacac
     8581 atacaataca tattatttcc acataagaac caaaaaacct ctatattaat atttcgtatc
     8641 aaaaaaaatg catctcccga tgcacttttt cttttacttt tattttattg cttcttcttt
     8701 aatttaatct tccttgctgc taaaaaccct atggcggcaa tcactccggc aacaaacaga
     8761 taaggtattg cggcaaccag cactatcagt gtcttctgaa acccctccac cacgttgttg
     8821 accgctttta tgaacccctg atatgctttt tcccacgttc cggggacatc cacactttta
     8881 agctcttccg gcttaagctc tctcatgtat acatagatag tggacagctg aaccaggtta
     8941 tcccagcgtt taaggtttcc tgagttaata tcgatgtcag tccttaccct gttcagttcc
     9001 gattcaattt tcagtatctc atccacagtc ttagcctttg ccataagctg ctgcaggctc
     9061 tgttcctgaa tcttcagatt atccaccctt gctgtcgtat ccctgtattc cgctgttata
     9121 tcgctgccgc ttatattttc gctgaccagt ttgcccatgc ctttaatatt gttgaaaata
     9181 tcctcaaatc tattttccgg aacccttaca gtcatatttg catacttctg tgtcgtctcc
     9241 ctggcctcag aacctgcatc atagacagga aactgcggca ctgtgacatt atcaatctgg
     9301 gaattctcca catagcctcc gctgctttca gtcatctgcc taatgtcagc ggctgcctta
     9361 tctacatttt ccacattcac tgatatgttg cccgatctta ttaccattct gcctgatctt
     9421 gcgctctcag cagcctggcc ctccaggccc tgactgggcg ccatttcctt tgccataagg
     9481 ctttcattga attgcatgcc gacagaatcc tctcccatac cccgctcctc tgccgcagcc
     9541 gtttcgaatt ctctcgactt ggctttgttc tcggcaaggt ctgagtcata ataaaccggc
     9601 ggtgccgcag gtgcttcagg cattgcagca ggtgcgggag ctatgccata acccggtgca
     9661 gaatcagcgc tctgtgccat tttcatgcta ttatcaaaaa gtaaggatga acctcctatt
     9721 cctatcgcaa ttaccagtgc tgcggcaaca agtcccgctg ccatcttcat gcgtttgctt
     9781 cccattatgc cgctgaagaa attcctttta cttttctcct cattcaacct ttggtgaagt
     9841 tccgttctga agttctgagg cagttcaatt tcttcgagat tgccgcatgc agcaattatg
     9901 tcagtcaggc tctcatattc cgccctgcat tcttcgcatt ccgcaaggtg cttttcgaat
     9961 tcggcctttt ctatattatt caaatcctta tctatatatg aagacatcat attctttatt
    10021 gtttcgcact tcatagccct tctacccccc ttcaccaaat tagactatat tttcccggga
    10081 aagttccatg ctttctttca gcttatccct gagcatggtt ctcgctctgt taattcttga
    10141 ctttacagta ccaagggagc agtcaagcat gccggatatt tcatcatagc tgaagccctg
    10201 tatgtccctt aatactatta cagttctata atcctcattc atttcccgta ttgcattcct
    10261 gataagttcc cgctgttcaa tcctttcata aacactttca ggggtttcct tgtcagaaca
    10321 caactccatg tgcagctcac cgtcttccgt atgtattgag ctgtccatgg atacatactt
    10381 ttcattcttc ctgcgcctta attcatccag gcaaacattg gtcacaattc tatacagcca
    10441 tgtagaaaag gccgcttgtc ccttaaaatc cttcaaagat ttgaataccc tgagcagtgc
    10501 ctcctgtgcc atgtccttcg catcttcttc gtttcccata atacggtaag caatattgta
    10561 tgccctttta tcataggagc ttatcaggag ttcaaaggat tcaacatctc cggactggct
    10621 cttctttatt aacagctttt ccaggtcact cacagcattt ccccctctcg ctatttaaat
    10681 acatccgtca tatgcatttt gacaaaccaa aaccccatgt gtcattcaat ctaatatcat
    10741 tttcaatatt taatgcccaa ggaggcacta ataatataga cataatcatt atgttattag
    10801 acgtaaaatt ttaaaaaaag ttcgggactt ccggcaaaac tttcaatata tttttaatca
    10861 gcgtcttttc tgctctttgc agcttgctga caagtatatt atactactaa ttttccttta
    10921 gtttaatatc aattaacgta taaaaatcat ttacagtata gtcatgtcct tccaaaaaag
    10981 tttccaggtc tattttttca tcgatattct gaccctgagc ggaaaaggtt tcaacagccc
    11041 ggccgcttct ttcatatatg ctgtaagctg tttccgcatt cagcaccagt tctccttcaa
    11101 tattttctgt tttaaaatac tctttgccaa cctttaaata aaccggcaac ttgagatcca
    11161 cgatcctgta acgtgaattc gcatctttag agcctcccaa tgcttcggca agattatccg
    11221 cattaataac ccatgcattg tttctcttaa gcttcattat cccttttcca aaaatcctta
    11281 tatcaacaga gtcttcggaa tactcatatc caaggttttt aaccgcttca acggtcaaat
    11341 agtccgattt gtcgtaatcc ccatgattta ttcctgcgaa aaccccgttg accttgacaa
    11401 cttccatgtc catatatgca ttaccggccc cttcatcata actcacagtc cctccgaagc
    11461 tatccgccgt atcattgagg tttatgtata tcaccccttc cagttcctta atccctatat
    11521 taaatttgcc ggccaattcc ggagtcaatc ccagaatatc ctctgcagct ttgtaattca
    11581 catatatttt tccctcctcc tccagagcat cgcaggtaat caggctgttg ttcaggaaca
    11641 ctccgattcc ttcggatacc gccattggct tggcgacggg ctttgctata atctcccggg
    11701 cttttttcaa aatttcttcg ggtatatccg tattactcaa ctgcgccaat tgctgtctcg
    11761 caatttccgc acctttttta taaatatccg gatatattat tatgctcttt ttcattgaat
    11821 acttatcttc aaacaacttt agcctgtcat attttatatt tacaggggta tttaccgtta
    11881 cctgattata tacttctacc acatccctgt tgaacattct tatgcaccct gcagatgcat
    11941 aggttccaat agactccggt ttgttattcc catgaatgcc tatgcccctg gtgattccca
    12001 tccaccttac gcccagagga ttttgaggcc cgggaggcac tacgtcaccc ttgttcagcc
    12061 aataggggtt aacagccttg tatattatcc tgtattgccc ggccggcgtg ggagttgact
    12121 gttttccaag gcataaggga tactccttgg ccagattgcc atctttgtac aagctcagag
    12181 tgccggaagg caaattaatc tcaatggctg acttatttga accgctgtct gccaaaaccc
    12241 ggctgaaaca aataataaca ataagtgcaa taaaaaaaga cctcttcaaa ataaatcccc
    12301 ctttaataca agtacacaca atctatattt attattggat ttataaaatt attcgaatct
    12361 gatgaatatt agaagaataa tgcgacg
//
LOCUS       DAOOAB010000064        11726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_6994_length_11726_cov_14.769086, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000064 DAOOAB010000000
VERSION     DAOOAB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_11726_cov_14.769086"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKU88_11160"
     CDS             <1..422
                     /locus_tag="PKU88_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HPX69867.1"
                     /translation="YLKSSAVDISGYQWAVSPMESYAKGNKELTVDGERGEYKIYWDR
                     NSPTGIPSLTILLDEKVILEKDMNEYIDKITSKYPPGGEGSHEADIEDMSLVLEVPEV
                     TVMLVFDNIDINVNPQEDIINYWMNLRSLYMKEKPQL"
     gene            complement(419..2272)
                     /locus_tag="PKU88_11165"
     CDS             complement(419..2272)
                     /locus_tag="PKU88_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69868.1"
                     /translation="MKRLILTLSVIIALLSGCTVKQGQLVVIENESPNEIRQDKSVYE
                     HLNVESRIFPIEKYKEAIGKIEAGMIPLAVSPDYSMVVAYQPTGSPVPDITNRRVVIG
                     NMVQEINLYICSTENGESKSLGKFLSLKDFHFDETGNSLAFIDGNSNVYIYSSITGQL
                     QKLVTAEKRDAYNILSWSKDSKSLMINTRMEFDIASREFISIAVNSYTPFIRSKFIDS
                     NYIVQMKNNEYNDMIAFYDFSTKEFTSIANGIYTDSDNSNVLYTKDYMSGLNIVSLKT
                     LESKSIENGPVYCSYIMKSTGDILYSTINNDLDSSYRYLLVKVNPHTMAKTSVKLDSP
                     TFHMSPAEDKLYVISSHSSNSIEIDAKELKVHRNESMKDDPDLYGIKSTLLKMFQLDY
                     NFNETYEKYETIAKEIYINTYDPLPQEALENKLIDFKRYNMPMPTSQREDHIPPTIYF
                     DQLNITDSKASINLGLYYINSVEMVKEDGNWYITGFSTHPASGEIASVTSIVKKHLYN
                     IKNKNINEAVKYWTAKKDTEYLEAQRKIVEELIDRADELTFEVGEIDLWSMSDPHRAE
                     SPDRATHAKVKILINDGNKTTKYKLILSRQYKKQFEINSWNADPLSISQLY"
     gene            complement(2287..3693)
                     /locus_tag="PKU88_11170"
     CDS             complement(2287..3693)
                     /locus_tag="PKU88_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX69869.1"
                     /translation="MKLKHKIIALNMGALFFMLIILGTIITAITDNYNLSTTLKYLEN
                     QGNYSAIYIEQYALSKAPNDFEVPSVMDASANYLAAVLNDLVKCRVQIYYGTELLGDS
                     GESTDFNGEIRPEIQETFKMKKAYFVAAGKNRTFYYAVPVSISSRYTYSLGFIYDLSE
                     VDNMKNNTVRMFVFTGVFLSIFMMLGSTIISNRITQPIKMLNEATRQFSEGNFESRAC
                     TATRDEVGELSVTFNSMADSIEDMINKLNHEKEKQKYFFDNFTHEIRTPLTTILGYAD
                     LLWKTNDENVKDKSLFYITSEGKRMLKMMERLLELSKLKNYNFELNKSDSNLKKLIED
                     ACDSMHYKMKRYNTSCRLDLEDITWKVDPDFFKQVIINLIDNSIKYSKSPVIDISLRK
                     KDKVVLEVVDYGCGIDIKNLESLFEPYYKVEKSRNSHNEGWGLGLSIVKEIIDKHGGS
                     IDIRSTAGKGTSIIITLE"
     gene            complement(3690..>4349)
                     /locus_tag="PKU88_11175"
     CDS             complement(3690..>4349)
                     /locus_tag="PKU88_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX69870.1"
                     /translation="IVDDEPAIVDLIKINLELEGFDTCVCYTGKQAVKAVPIFMPDLI
                     LLDIMLPDINGFEVCKKLQDLSIPIIMLTAKNDIKDKLYGLELGADDYITKPFDSREL
                     TARIRTVLKRINKSEPKDDSNIQLGPIMINTSERQVYVDEEEIVLTPKEYDLLLVLCE
                     NQRKVFSRENLLELVWKFEYIGDSRTVDMHVQRLRKKLGKYSFFIKTVFGIGYKFEVQ
                     V"
     assembly_gap    4350..4449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4450..4848)
                     /locus_tag="PKU88_11180"
     CDS             complement(<4450..4848)
                     /locus_tag="PKU88_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017554691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPX69871.1"
                     /translation="MKDLIMKEIRALKEQLCEISDYICRNPELGNHEFKAVEKLTTFL
                     ESKGFSVETNIVGKPTAFKSIFDSKKPGPAIAFLCEYDALPEIGHGCGHNMIGTMSCG
                     AAVGLSKVLYETGGRITVLGTPAEETDGAKV"
     gene            4983..6347
                     /locus_tag="PKU88_11185"
     CDS             4983..6347
                     /locus_tag="PKU88_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX69872.1"
                     /translation="MTQATEKKSLLSGILTLSIPTMLGMMFDMLYELVDMAWVAMLSI
                     NSVAAVTIFATIWWILNMVNDIVGTSSVTIISRYFGAGNREKTIEAIEQTIIFKFLLA
                     LIVGILMSFIIPYILSPLAGNSQVLEESVLYGRIRLITLPLAFSSYSVNTALRCIGDA
                     KKPLYIMAVSGLLNMILDPIMIFDVIPFIGVRGMGLGIAGAAYATVISQAAAFIVGLY
                     ILMSGKTFIKVGFKRGIYFVKAMDIKLLTIGLPTGLEGLLRNFGYFIVMRFIASFGVG
                     VVAAYGICIRIVNLVLMPVFGLQMGSGVIVGQSLGSSDCDKAEKAGYLTAKLSFWIMV
                     ASGVILALFPGPVMRIFTTGDDIIGIGSVFLRYYSVAALFMGPAGGLASILYGAGDNV
                     PSMLAALASVWLVQIPMLYLFVQVLQMSVEWVWISYIVSYAVHFAVIVYYVKKGRWKD
                     KCVI"
     gene            6409..8220
                     /gene="pepF"
                     /locus_tag="PKU88_11190"
     CDS             6409..8220
                     /gene="pepF"
                     /locus_tag="PKU88_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPX69873.1"
                     /translation="MMKNKGTGIPKRDELSQEFKWKLEDIYASNDLWEKDFQRVKQMA
                     DFLLPYKEIMAESADKLLECLNMSTEMNRLFEKVYVYAHMRSHEDSTDSFYQGLADRA
                     DSLGVTVSSAGSFIVPGILAVPDGKLEAFLMESEGLRFYKRFLDEIRRKKPHILNASE
                     EQILALAGEMARAPETIFGMLNNADIKFPHIKDEKGNELELTKGRYIQLMESSDRRVR
                     KDAFEGMYITYSKQKNTLAALLNANVKANMFSARVRKYGSAREESLFEDNVPAAVYDN
                     LIAAMHDNMNLMHRYVKLRKRIMGLEELHMYDLYVPMIKEVKMEIPFEEAKKIVRKGL
                     EVLGEDYGKDLEKGLNSGWIDVYENEGKRSGAYSWGCYDSHPYVLLNHNDTLNNMFTL
                     AHEMGHALHSYYSDSNQPYIYAQYKIFVAEVASTVNESLVMNYLLNTTNDQKKKMYLL
                     NYFMEQFRTTVYRQTMFAEFEKIIHEKAESGEALTAELLCSIYRDLNIRYYGPDIIVD
                     DYIDMEWARIPHFYSSFYVYKYATGFSAAMSISQQMLKEGRPAVDRYMEFLKSGGSDY
                     PIELLKIANVDMSTKEPVDNALKLFGSLLDQMEELTS"
     gene            complement(8312..9550)
                     /locus_tag="PKU88_11195"
     CDS             complement(8312..9550)
                     /locus_tag="PKU88_11195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPX69874.1"
                     /translation="MKTVPFSKSELEHIASTFHTPFYIYDETAIRNNVSRLKNAFHWN
                     PGFKEYFAVKSTPTPGILKLFRQEGCGAECASLTELMLAERCGFEGRDIMFTSNVTTV
                     KEYQYASRLGAIINLDDAAHIPILEACTGIPETICFRLNLGDQLKYGESVLIDFTNSK
                     FGSTKLQLEAAYPAVRNLGAKHFGIHAQFGCHKRNPEFFGINARKVFAFAVELFEKTG
                     IVIDFVNLAGGIGIPYHAEDEPADIEAISLQIKRAYDETIGESTLPPVSLYTELGIFM
                     TGPYGYFVSKVLHVKQAHKTFIGLDASTNSFMSPLKYNNYHHITIMGKESAEPVQHYE
                     ITGALCEDRDRFATDRLLPQITAGDLLVFHDAGAYGYSHGNNFNGRLRPAELLLHGNG
                     DVSLIRRHETPDDYFSTMIF"
     gene            9659..10975
                     /locus_tag="PKU88_11200"
     CDS             9659..10975
                     /locus_tag="PKU88_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPX69875.1"
                     /translation="MYKYRNIADLLKKEIYSGNCKPGSKLPSIQRLSKETGLNSDTIV
                     KAYKELVQEHIVYAVPKSGFYVAKNKLGTGGSIIDMKNVCLPEGINPYKDFQLCIEQA
                     MAIYGQRLFEYGSPKGLPELLQVLPKHLLGLQVFAKAENICITNGSQQALYILSAMQF
                     PHGGARVLVEQPTYSLMLRILENARIPAAGIRRTRDGIDLEQLKKLFQQGDIKFFYMI
                     PRYHNPTGFSYSNPQKTEILKLAEQYGIFIVEDDYLADLEVDQKAETLHTLGNKDRIV
                     YIRSFSKTLLPGLRLGMAVLPEFLQKQFLDFKSSMDLNTSILTQAALEIFLRSSMYRF
                     HAKRTKEHYKNKMSRLQEYCKEAKGCGTWYIPQTGLYAYLETGGISSQLLERGLLDSK
                     VLVSSTNQSYLNDFEHPEGLRLCVCNAEDQEIEKAVQIIETKLTEF"
     gene            11149..11224
                     /locus_tag="PKU88_11205"
     tRNA            11149..11224
                     /locus_tag="PKU88_11205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11182..11184,aa:Ala,seq:ggc)
BASE COUNT         3220 a         2228 c         2663 g         3515 t
ORIGIN      
        1 tttatctgaa atcaagtgct gttgacataa gcggctatca atgggcagtc agccctatgg
       61 agagctatgc aaaaggaaat aaagagctta ccgttgacgg tgaaagagga gaatataaga
      121 tttattggga taggaattct cccacaggga taccgtcatt gacgatattg ctggatgaaa
      181 aagtgatttt agaaaaggac atgaatgaat atattgataa aataacttca aaatatcctc
      241 ccggagggga aggatcccat gaagcggata ttgaggatat gagccttgtt ctcgaggttc
      301 cggaagtcac tgttatgctt gtttttgata atattgacat aaatgttaat ccccaagaag
      361 atatcataaa ttactggatg aatctgcgct cattatatat gaaagagaaa ccgcagcttt
      421 aataaagctg cgatatgctc agagggtctg cgttccagct gtttatttca aactgcttct
      481 tatactgtct cgagagtata agcttgtatt ttgtggtttt gtttccgtcg tttatcagta
      541 tttttacctt tgcatgggtg gctctgtccg ggctctccgc cctgtgaggg tcactcatgg
      601 accaaagatc aatctcacct acttcaaagg taagctcatc ggctctgtct ataagctcct
      661 cgacaatttt tctctgggct tccaggtatt ctgtatcttt tttggctgtc caatacttca
      721 ctgcttcatt aatgttcttg ttctttatat tgtaaaggtg cttcttaaca attgaggtta
      781 ccgaggctat ttctccggag gcagggtggg tcgaaaaacc tgttatatac cagtttccat
      841 cctccttaac catttccacc gaattaatat aataaaggcc aagatttata gatgctttgg
      901 aatctgtaat attaagctgg tcaaaatata tggtcggagg tatatgatcc tcacgctggc
      961 tggtcggcat tggcatattg tatcttttga agtcaatcag cttattttcc agtgcttcct
     1021 gaggaagggg atcataagta ttgatatata tttccttggc aatagtttca tacttttcat
     1081 aggtttcatt gaaattataa tctaattgaa acattttcag cagggtggac ttgattccgt
     1141 acaaatccgg atcatccttc atgctttcat tcctatgaac tttcagttct tttgcatcaa
     1201 tttcgatgct gtttgaactg tggctgctta taacatacag cttatcttcc gcggggctca
     1261 tgtggaaggt gggagagtcc agcttgacac ttgtttttgc cattgtgtgg ggattcacct
     1321 ttaccaataa atatctgtag ctgctgtcaa gatcattatt aatagtgctg tacagaatat
     1381 cacctgttga tttcattata taagagcaat atacggggcc gttttctata gatttgctct
     1441 ccagggtctt caggcttact atattgagcc cgctcatata gtctttggta tacagcacat
     1501 ttgagttgtc cgaatcggta tagattccgt tggcaatgct tgtgaattct ttggtgctga
     1561 aatcatagaa agcaatcata tcattgtatt cattgttttt catctgcaca atgtaattgc
     1621 tgtcgataaa cttgctcctg ataaacgggg tatagctgtt tactgcgatt gatatgaatt
     1681 ctcttgaagc gatgtcaaat tccatacggg tatttatcat caggcttttt gaatcctttg
     1741 accagcttaa gatgttatag gcgtcccgtt tttccgcagt gaccagcttt tgtagctgtc
     1801 cggttatgct gctgtaaata tatacattgc tgtttccgtc aataaaagcc agggaatttc
     1861 ctgtttcatc aaaatgaaaa tcctttaagg agagaaattt cccgaggctt ttgctctcac
     1921 cattctcggt gctgcaaatg tagagattta tttcctgcac catattccct atgacaactc
     1981 tgcggtttgt gatatcagga acagggcttc ccgtaggctg gtatgcaacg accatactat
     2041 agtcaggcga aactgcaagg ggaatcatgc ctgcctcaat cttgccaatt gcttctttgt
     2101 atttttctat aggaaatatc cgggattcca cattaaggtg ctcgtaaaca cttttatcct
     2161 gccggatttc attagggctc tcattttcaa tgacaaccag ctgcccctgc tttactgtgc
     2221 atcccgagag caatgctatt ataactgaca atgtcaggat taatcttttc ataaaaaaac
     2281 cccctgctat tccaatgtta ttattatact ggtgcctttc ccggcggtgc ttcggatatc
     2341 aatactgccg ccgtgcttat caattatttc ctttactatg gacaagccga ggccccagcc
     2401 ctcattatgg ctgtttctgg atttttccac cttataatag ggttcgaata agctttcaag
     2461 gtttttaata tctattccgc agccgtaatc cacaacttca agcaccactt tgtctttctt
     2521 cctgaggctt atgtctatga ccggagactt gctgtacttg attgagttgt caatcaggtt
     2581 tattattacc tgtttgaaga aatcaggatc caccttccat gtgatatctt ccagatctag
     2641 cctgcagctt gtattgtacc ttttcatctt ataatgcatg gaatcgcatg catcctctat
     2701 cagcttctta aggtttgaat cggacttgtt cagttcaaag ttgtaattct tcagctttga
     2761 aagctcaagg agcctctcca tcatcttcag cattcttttc ccttccgaag taatgtaaaa
     2821 caggcttttg tccttcacat tctcatcatt ggttttccac aacaggtcgg catatcccag
     2881 gattgtagta agaggagttc ggatttcatg ggtaaaatta tcgaagaaat atttttgttt
     2941 ttctttttca tgattaagct tgtttatcat atcttcaata ctgtccgcca ttgaattgaa
     3001 ggtaaccgaa agctctccca cctcatctct ggttgctgta caagcacggc tttcaaagtt
     3061 gccttctgaa aactgcctgg tagcttcatt cagcattttt atcggctggg taatcctgtt
     3121 tgaaattatt gtgcttccca gcatcatgaa tatagacagg aaaactcccg tgaagacgaa
     3181 catcctcact gtattgttct tcatattgtc aacttcgctg aggtcgtata tgaatcccag
     3241 ggagtaggta tatctgctgc tgatgctcac gggtactgca taatagaagg ttctgttttt
     3301 tcctgccgca acaaaatagg cctttttcat tttaaaggtt tcctggattt ccggccttat
     3361 ttctccgttg aagtccgttg actctccgga gtccccaaga agctcagtgc cataatatat
     3421 ctgtactctg cattttacca ggtcattgag gacggctgcc aaataattgg cgcttgcatc
     3481 catgaccgaa ggtacttcaa agtcgttggg agctttgctc agggcatact gttctatata
     3541 tatggctgag taattcccct gattttcaag atacttgaga gtggtggaaa ggttgtagtt
     3601 gtctgttatt gcagtgatta tcgttcccag tattatgagc atgaaaaaaa gagcacccat
     3661 attaagtgca atgattttat gcttaagctt catacctgta cctcaaattt ataaccgatg
     3721 ccaaatacgg tttttataaa aaagctgtat tttccgagtt tctttctcag ccgctgcaca
     3781 tgcatgtcca cagttctgct gtcaccgatg tattcaaatt tccacaccag ttcaagaagg
     3841 ttctccctgg agaagacttt tctctgattt tcgcagagaa ccagaagaag gtcatattcc
     3901 tttggagtca gtacgatttc ttcctcatcc acatagacct gccgctccga tgtgttaatc
     3961 attatgggcc ctaattgtat attggaatca tccttgggtt ctgatttatt aattcttttg
     4021 aggacggttc taatcctcgc ggtcaattcc ctactgtcga agggcttggt gatatagtcg
     4081 tcggcaccca gttcaaggcc ataaagcttg tccttaatgt cgttcttcgc cgtaagcata
     4141 ataattggta tgcttaaatc ctgaagcttc ttgcagactt caaatccgtt gatatccgga
     4201 agcattatat ccagcaatat caagtcgggc ataaaaatcg gcacagcttt gacagcttgc
     4261 ttgccggtat agcatacaca agtatcgaag ccttccagtt cgagatttat ctttattagg
     4321 tcaacaattg ccggttcatc atcaactatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnc acctttgctc cgtctgtttc ttccgccgga gtgccaagta ctgttatgcg
     4501 acctcctgtt tcataaagaa ccttgctgag acctactgct gctccgcagc tcatggttcc
     4561 tatcatatta tgtccgcagc cgtggcctat ttccggcaga gcatcatatt cgcacagaaa
     4621 agctattgca ggtccgggtt ttttactgtc aaatatagat ttaaaggctg taggtttgcc
     4681 tacgatattt gtttcaaccg agaacccttt tgattcaagg aaggtggtca gcttttcaac
     4741 tgccttgaat tcatggtttc ccaattccgg attgcggcat atgtagtcgc ttatttcgca
     4801 aagctgctcc ttaagtgccc ggatttcttt cataattaag tctttcatgg cttcctccga
     4861 tttattatct ttttatgcct atattataca atattatata gttttactca cataaaattt
     4921 gatatagaaa aaatcactca taagaggtag aataaggata tgattaaaat acggaggatt
     4981 atatgacgca ggcaacagag aagaaaagct tgctttcggg gatattgacg ctttccatac
     5041 ccacaatgct tggtatgatg ttcgatatgc tgtatgagct ggtagatatg gcatgggtag
     5101 caatgctgtc aataaactca gtagcagctg ttacgatttt tgcaactata tggtggatat
     5161 tgaatatggt gaatgatatt gttgggacaa gctctgtaac tataatatca agatacttcg
     5221 gtgcgggaaa cagggaaaag accatagagg ctattgaaca gacaataata tttaagtttc
     5281 tgctggcatt aatagttggt attttaatga gcttcataat accatacata ttaagtcccc
     5341 tcgccggaaa cagccaagtg cttgaggagt cggtgctata tggaaggata aggcttatta
     5401 cacttcccct tgctttttct tcttattccg taaacactgc cttaaggtgc ataggagatg
     5461 caaaaaagcc tttgtatatt atggcagttt ccggtctttt gaatatgata cttgacccca
     5521 taatgatatt tgatgtcata ccctttatag gagtaagagg aatgggattg ggaatagccg
     5581 gagccgccta tgcaacggta atatcccagg cagccgcctt tattgtgggt ctgtacatac
     5641 ttatgtcagg taagactttt ataaaggttg gcttcaagag gggaatctat tttgtaaagg
     5701 ctatggatat caagctgttg actataggct tgccaacagg cttggagggt cttctcagaa
     5761 attttggata ttttatagtt atgagattta tagcatcttt tggagtaggt gttgtagcag
     5821 cttacggcat atgcataaga atagtaaatt tggtgctgat gccggtattc ggtttgcaaa
     5881 tgggctcggg agtaatagta ggtcagagct tgggttcatc cgattgtgat aaggctgaaa
     5941 aagcgggata tttgacagca aagctttctt tctggataat ggttgcttca ggtgttatct
     6001 tggcattatt tccgggaccg gttatgcgta tttttacgac aggggatgac ataataggta
     6061 taggctccgt attcttgaga tattattcgg ttgcggcgct gtttatgggc cctgcaggtg
     6121 gattggcttc aatactttac ggagcggggg acaatgttcc ttctatgctg gctgccttag
     6181 cctcggtttg gcttgttcag ataccgatgc tgtatttgtt tgtgcaggta ttgcaaatga
     6241 gtgttgagtg ggtatggata tcatacattg tatcttatgc tgttcatttt gcggttatag
     6301 tatattatgt aaaaaagggc aggtggaagg ataaatgtgt gatatagtca cgagatacga
     6361 gggttagggc aaggctttgg agagatttat ataataaagc gaggggtaat gatgaaaaat
     6421 aaaggcacag gtattccgaa aagagatgaa ttatctcagg aatttaagtg gaagcttgaa
     6481 gacatatatg cttcaaatga tttgtgggag aaggacttcc aaagagtaaa gcaaatggcg
     6541 gatttcctgc tgccttataa agaaatcatg gctgaaagcg ccgataagct gctggaatgc
     6601 cttaatatga gtaccgaaat gaacaggctt ttcgagaagg tatatgtata tgcgcatatg
     6661 agaagccatg aagacagcac cgattctttt tatcaggggc ttgccgacag agcggattcc
     6721 ctgggggtaa cagtaagcag cgccggttct tttattgtac cgggtatcct tgctgtgccc
     6781 gatgggaagc tggaagcttt tcttatggaa agtgaaggct taaggtttta taaaagattt
     6841 ctggatgaaa taaggagaaa gaagccccat attctgaatg cgtccgagga gcagattctg
     6901 gccttggccg gggaaatggc ccgggcaccg gagacgattt ttggtatgct taataacgcg
     6961 gacataaagt tcccgcatat caaggatgaa aagggaaatg agctggagct tacaaagggc
     7021 aggtatattc agttaatgga aagcagcgac aggagagtaa ggaaggatgc atttgagggg
     7081 atgtacatca cttattcaaa gcagaagaat actctggcag cacttctcaa tgcaaatgta
     7141 aaggcgaata tgttcagcgc aagggtgaga aagtacggct cagcgcgaga ggaatccctt
     7201 tttgaggaca atgtgccggc agcggtatat gacaacctta ttgcagccat gcatgacaat
     7261 atgaacctga tgcacaggta tgtaaagctc aggaaaagga taatggggct tgaagagctg
     7321 catatgtatg atctgtatgt tccgatgata aaggaagtaa aaatggaaat accttttgag
     7381 gaagcaaaga agattgtcag gaaagggctg gaggtacttg gagaggatta tggtaaagac
     7441 cttgaaaagg gcttgaactc aggctggata gatgtttacg agaatgaagg gaagagaagc
     7501 ggtgcatact catggggctg ctatgactca catccctatg tgcttttgaa tcataacgac
     7561 accttgaaca atatgttcac cctggctcac gaaatgggtc atgcgctgca cagctattat
     7621 tccgacagca atcagcctta catttacgca cagtataaga tttttgtggc agaggtagct
     7681 tcgactgtca atgaatcctt ggtaatgaac tatcttctga ataccacaaa tgatcaaaag
     7741 aagaagatgt atcttctgaa ctattttatg gagcagttca ggaccactgt atacaggcaa
     7801 accatgtttg ctgaatttga aaagattatc catgaaaagg ctgagtccgg agaagcattg
     7861 actgcggaac tattgtgctc tatatacagg gacttgaata taaggtatta tggaccggat
     7921 ataattgtgg atgattatat agacatggag tgggcaagaa ttccgcattt ttacagcagc
     7981 ttctacgtat acaaatatgc aacgggtttt tcggcagcca tgtccatatc acagcagatg
     8041 ctgaaagagg gcaggcctgc ggtggaccgc tatatggaat tcctgaaaag cggcggctcg
     8101 gattatccca tagagctttt gaagattgcc aatgtggaca tgtcgacaaa agagccggtg
     8161 gacaatgccc ttaagctgtt cggcagcctt cttgaccaga tggaggaact gacttcttag
     8221 taggtgccag gcaccgaaca aaagaactta attgtcggtt aataagaaag aagttctaaa
     8281 gttcggtgcc tggcatcgtt gatctacttg gctaaaaaat catagtcgaa aaatagtcgt
     8341 cgggggtttc atgccgtctg atgagactga catcgccatt accgtgaaga agaagctctg
     8401 ccggccgcag cctgccattg aagttattgc cgtgactata gccataggca cctgcgtcat
     8461 gaaacacgag caggtcgcct gcggttattt gggggagaag cctgtcggtt gcgaagcggt
     8521 cccggtcctc gcaaagcgct ccggtaattt catagtgctg aacaggctct gccgattcct
     8581 ttcccattat ggtgatatga tggtagttgt tatattttaa cgggctcata aaactgttgg
     8641 tggaagcatc caagccaata aaagttttat gggcttgctt cacatgaagg acttttgaaa
     8701 caaaatatcc gtatggcccg gtcatgaaaa tccccagctc tgtgtaaaga gaaacagggg
     8761 ggagagtgct ttcgccgatg gtttcgtcat aagctctctt tatttgcagg ctgattgctt
     8821 caatgtctgc aggttcatcc tcagcatggt aaggaatgcc gatgcctccg gccagattta
     8881 caaaatcaat gacgatgcct gtcttctcaa atagctccac agcaaaggca aaaaccttac
     8941 gggcatttat gccaaaaaac tcaggattgc gcttatgaca tccgaattgt gcatgaatac
     9001 caaagtgctt cgctcctaaa ttgcgcactg cagggtaggc ggcttcaagc tgaagttttg
     9061 tggagccgaa cttgctgtta gtaaaatcga taaggacgga ctcaccatat tttagctgat
     9121 cgccaagatt caatctgaag cagatggttt ccgggatacc ggtgcaagcc tccaaaatgg
     9181 gaatatgtgc tgcatcatcc aggttaataa tggcgccaag ccgggaggca tactgatact
     9241 ctttaacagt tgtgacattt gaggtgaaca tgatgtcacg gccttcaaag ccgcaacgct
     9301 ctgcaagcat cagttcagtc agggaagcgc attcggcgcc gcagccttcc tgccggaaaa
     9361 gctttaaaat tccgggtgta ggtgtggatt ttactgcgaa atattccttg aaacccggat
     9421 tccaatgaaa ggcattctta aggcggctaa cattgtttct aatggcagtt tcatcatata
     9481 tatagaaagg tgtatgaaaa gttgaagcaa tgtgttctag ttctgatttg gaaaacggaa
     9541 cagttttcat cttttattac tccttttttc atgtgatata ataattataa ataaaatacc
     9601 gatataaaaa ccagtataga aatacatgag tataccggta tagatgggtg gccgtattat
     9661 gtataaatat aggaatatag cggatttgct caagaaggag atatattcag gaaattgcaa
     9721 gcccggttca aagcttccct cgattcaaag gctgtcaaag gagacgggcc tcaacagcga
     9781 tacgatagta aaggcctata aggagctggt acaggagcat atcgtgtatg ctgttcccaa
     9841 aagcgggttt tatgtcgcga aaaataagct tggcaccggt ggcagcataa ttgacatgaa
     9901 aaatgtatgc ttgccggagg gaataaatcc gtataaagac tttcagcttt gcatcgaaca
     9961 ggctatggcg atttatgggc aaaggctatt tgaatacgga tccccaaagg ggttgcctga
    10021 actgcttcaa gtactgccta agcacctgct tggccttcag gtttttgcga aggctgagaa
    10081 catatgcatt acaaatggtt cgcagcaggc cctgtatatt ctgagtgcaa tgcagtttcc
    10141 gcatggcggt gccagggtgc ttgttgaaca gcctacttat tcattgatgc ttcgaatatt
    10201 ggaaaacgca aggatacctg ctgcaggaat ccggagaacc cgggacggaa ttgatctgga
    10261 gcagcttaag aagctgtttc agcaggggga tataaagttt ttttacatga ttccaagata
    10321 tcacaatcca acagggttta gttacagcaa cccgcagaaa acggagattt tgaagcttgc
    10381 agagcaatat ggcattttta ttgtagaaga tgattatctg gcggaccttg aagtggatca
    10441 gaaggcagag acgctgcaca ctttgggaaa taaggatcga atcgtctaca taagaagctt
    10501 ttcaaagacg ctattgccgg gtttgagact tggaatggca gtccttccgg agtttttaca
    10561 aaaacaattc ctggatttca aaagcagtat ggatttgaat acttccatac tcacccaagc
    10621 ggcattggag atatttttga gaagcagtat gtaccgtttt catgcgaagc gtacaaagga
    10681 gcattataaa aacaagatga gcagactgca ggagtattgc aaagaggcga aaggctgcgg
    10741 cacctggtat atacctcaga cagggctcta cgcttatctt gaaacaggag gcatttcttc
    10801 gcagttactt gaacgaggac ttctggacag caaggtcctg gtcagcagta ccaaccaaag
    10861 ctatttgaac gattttgaac atccggaagg attgaggctt tgcgtatgca atgcagaaga
    10921 ccaggaaatc gaaaaggcgg tgcaaattat tgaaacaaag cttacggagt tttgagataa
    10981 gtcatgaatt taggtgccag gcaccgaaca aaagaactta attgtcgatt aataagaaag
    11041 aagttctaaa gttcggtgcc tggcaccgtt tatccaaaac gaattgatta tttgtgttga
    11101 atttttctct gaaatgatat agaatatact ttgtcaaaac aaataagtgg gggtatggcg
    11161 cagctgggag cgcgcttgaa tggcattcaa gaggtcaggg gttcgatccc ccttatctcc
    11221 accaaatcta gtaccatcta ttgataaaaa gaattttatc ggtgggtggt tttattatgc
    11281 cctgaaaggg cttgaaatca ggggcattgt caggttgggg caggtaggtg aggttgttct
    11341 ggaggagtgt tttttccgaa acgatagagg ttgattaata tatttccgtg aacaggccag
    11401 gttgtatgtg gaaatatata ctaaagctct gattcgtgcc taatccaatc ctatttctca
    11461 ttatcccttt tgaatctata atataatcta agtttgtact tctttctact gctttgatta
    11521 aattcttttt atttcccact taactttgta tgaaactagt tatataatgt tattcataga
    11581 gaattcctca ttcggttgtg atttagtcga ttactattat ctcggatagg gagataattc
    11641 tctaaataat ttcaaaaaat gggaggatcc gatgaaatta aaagggaaaa ctgcagtagt
    11701 aacaggtgca agttcaggta tgggaa
//
LOCUS       DAOOAB010000065        11274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_7345_length_11274_cov_15.698369, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000065 DAOOAB010000000
VERSION     DAOOAB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7345_length_11274_cov_15.698369"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PKU88_11210"
     CDS             complement(<1..1466)
                     /locus_tag="PKU88_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPX69876.1"
                     /translation="MYFDNKVWIAKGDNPVYLLPNMANRHGLIAGATGTGKTITLKVM
                     AESFSDMGVPVFLTDIKGDLAGLSEQGSDNPNMAERIAKLGLEGFNYKSYPVCYWDVF
                     GKGGHPVRTTVSEMGALLLSRLLGLNDTQAGVLNIVFRVADENGMLLLDMKDLRAMLS
                     YVGENAKEYTLKYGNVSSQSIGAIQRSLLVLEDQGGAQFFGEPALDIYDWMRTDSEGQ
                     GYINILHCVELFHSPALYSTFLLWMLSELFETLPETGDVSKPKLVFFFDEAHLLFDDT
                     PKALLQKIEQVVRLIRSKGVGVYFITQNPMDLPQEVLGQLGNRVQHALRAYTPAEQKT
                     VKAAAETFRTNPAFDTETAITELGTGEALVSCLDSKGIPGMVERAFILPPQSKFGTID
                     DFTRLRIINSSSMSSKYDTEVDRESAYEMLQAKAMREQMAEKEEKEYTERQKQLEKEE
                     KERQKQLEAERKERERIKRKEETRKYSRKTLAERAADN"
     gene            complement(1720..2469)
                     /locus_tag="PKU88_11215"
     CDS             complement(1720..2469)
                     /locus_tag="PKU88_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPX69877.1"
                     /translation="MEAVKRFDVRKLVIVGVLGGVSAILGMTPLGFIPVGPTRATIMH
                     IPVIIGAIMEGPIVGALVGLIFGLFSVFQALTNPTPVSFVFLNPLVSIVPRVLIGITS
                     YYVYTALSNLGNRKTLGILNVIWIAIIGYLSYGIYKNIENSQSPWLIAVNIALIAFTA
                     AIAFLANRKFRGKALDLVISAIVGTMTNTVLVLSMIYLIYAESFVKSIGQDPALARKI
                     IMGIGIANGIPETIIAILIVTSVVGALKRAE"
     gene            complement(2644..3600)
                     /locus_tag="PKU88_11220"
     CDS             complement(2644..3600)
                     /locus_tag="PKU88_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPX69878.1"
                     /translation="MYPGYITDVKGIIVGHSQSEKGMTGCTAVICEKGAVGGVDVRGS
                     APGTRETDLLKPDKLVDRIHAVVLSGGSAFGLEAASGVMRYLEEQGIGFDVGVAKVPI
                     VASAVIFDLNIGDHRIRPDLNMGYDAARSASAEEGRQGNAGCGLGATVGKAFGPEHAM
                     KSGLGSASLKSGELIVSAIVSANSFGDIFDYETGRQLAGVYDYEKREMLNTYEIMKRE
                     GRVLGFPMRNTTIGVVAANAVLTKAEANKIAAMAHNGFARSINPVHTMMDGDTIFTMA
                     TNEIKADINLVGTMAAEAVSRAVTNAVVYAKSCHGLIACCDI"
     gene            complement(3650..4480)
                     /locus_tag="PKU88_11225"
     CDS             complement(3650..4480)
                     /locus_tag="PKU88_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69879.1"
                     /translation="MGRPWREEYKGGIYHVIARGNNKEYIFRESIDKGYFLKQLKDYS
                     VGMEYRIYGYVLMDNHYHIIIQTYNKKLQEIMHQINNKYSKYFNYKYERVGHVFQGRY
                     KAVLVQDERYMLSLLRYIHQNPIKAHICDSVEKYKWSSDVFYRNSINSYIHIEMIMDM
                     LSKDRKEAIKKYKEYMEQDEEIDYDNSKAIGEEAYQIMCLTRKQVKERKRLDEILIGT
                     GVSEEGYWLIKAGKRRMDLTDYKVKYAQEALSHKYSYKEIGDNIDMSDTGIRNLLKRN
                     "
     gene            complement(4570..4923)
                     /locus_tag="PKU88_11230"
     CDS             complement(4570..4923)
                     /locus_tag="PKU88_11230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HPX69880.1"
                     /translation="MVLRRGDIFYGDLSPVIGSEQGGVRPVLIIQNDIGNKYSPTVIV
                     AAITSQINKAKLPTHVELEALKYGITKDSVILLEQIRTIDKRRLKEKIGHLSEELMKQ
                     VDTAIQISFGLIEYT"
     gene            complement(4925..5200)
                     /locus_tag="PKU88_11235"
     CDS             complement(4925..5200)
                     /locus_tag="PKU88_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HPX69881.1"
                     /translation="MADLKKIAVCMPDTLLKEIDDMVCHKCINRSEFIRDAMRFYISE
                     IKRSNAVEKLRNGYLEMSEINVTLAEIGLTADYETIIRYETRLAECE"
     gene            complement(5394..6569)
                     /gene="alr"
                     /locus_tag="PKU88_11240"
     CDS             complement(5394..6569)
                     /gene="alr"
                     /locus_tag="PKU88_11240"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPX69882.1"
                     /translation="MYDLKEIRAAWAEVDLDALAHNMREIRRLARKDALVTAVIKADG
                     YGHGATKIAQTLLDNGADRFAIAVLDEGIELRNAGFGVPILILGFTDKERAEEIVSFN
                     LEQAVYSWELAEAISKAAVKQNKTAKIHIKVDTGMGRIGLKPDKDSVQLIKKISQLPN
                     IVIEGIFTHFAVADALDKAYTEEQYAKFTWICKELERENVRINVKHCGNSATIIDLPE
                     MHLNMVRAGIILYGLKPSDEVMLDRIELKQVMSLKVRISHVKEIEAGQSVSYGRRFIA
                     EKKSKIASLPIGYADGYTRMLSGKAEALVKGRRVPVVGRICMDQCMVDVTGIEDVKVG
                     DEAVLFGRQGEGFIHIDELAEKLDTINYEIVCMISRRVPRVYVKDGKIVDVLNYLVD"
     gene            complement(6478..7224)
                     /locus_tag="PKU88_11245"
     CDS             complement(6478..7224)
                     /locus_tag="PKU88_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69883.1"
                     /translation="MYRRIICVLIIVCAGLTGCGMIKQKKNFGEAGRRLLKLEAYTCD
                     VVMKVTNNRSTMEYRLKHSYKSPDKYRVEVLAPRELQGQVTIYNGHSSYIYHPGINQY
                     FVTEDFSGSVEYNSFIGSFMEHIRKTDEIKVSYEKYGDKECIVAEFEIPEPNRYMHFE
                     KLWIDRSEIVPLRAEIYGSDGKTYVEIFYDNFVYNPRLEDGDFEIIRKNSMKLQEMKR
                     NVRSEGNQGRMGGSGFGCSCPQYEGNKEAC"
     gene            complement(7288..7734)
                     /locus_tag="PKU88_11250"
     CDS             complement(7288..7734)
                     /locus_tag="PKU88_11250"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPX69884.1"
                     /translation="MIFGIGTDIVEIKRIKKAISRSPRFIERLFTAQELEFYKKKDMK
                     AQHIAGGFSAKEAVLKALGTGLRGFRWKDIEILRGPVGKPIVRFEGNVKQFIEDNGIG
                     IIHVTISHSREFATATAVAEVSMEREGMVIEDLFFQSHEEARKNIH"
     gene            complement(7884..8483)
                     /locus_tag="PKU88_11255"
     CDS             complement(7884..8483)
                     /locus_tag="PKU88_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69885.1"
                     /translation="MGKDISFKDNIVRRNKIPILIYVPEWIQLFSGNKSRSMQKIIAR
                     LEALIARNKECDSELYNLEKRKKMVMNKILYLSKDINENNDKTALSKMEEAEKEIKEI
                     NRKISVLLEELESIPKQINDMNTQLLKETIKRAYELINESRSESEKCQEQVNDIRQKL
                     AELIQKKADMEERVNKLYLFIHGMIGADEMESLDESFLP"
     gene            complement(8593..10269)
                     /locus_tag="PKU88_11260"
     CDS             complement(8593..10269)
                     /locus_tag="PKU88_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX69886.1"
                     /translation="MSKKVVIVGGVAGGASTAARLRRIDERAEIILFEKGQYISFANC
                     GLPYYIGGTIKERKKLLVQTAEAMEARFNIDVRELSEVKRIDRQRKVVEVKNLKDGRN
                     YEENYDVLVLSPGAVPVKPPIPGVDSRNIFTLRDIPDTDSIKEFLNEKKPKNAVIAGG
                     GFIGLEMAESLHSAGLKVDIVVAHEQVMSQLDYSMAAIVHNHIRSKGCGLHIKDGVKA
                     FEPEGDKTVAVLQSGKRIPADMIILSKGVRPNNSLAKEAGLEIGVTGGIKVNEYLQTS
                     DPNIYALGDAIEVTDYVSGKPALIALAGPANKQGRIVANNIAGRRESYKGTQGTAIAK
                     VFDIVAASTGVNEKALKKLGKELGKDYITTTTHSASHAGYYPGGKLMAVKIIYTPEGR
                     VLGAQIVGSDGVDKRIDYIAIAIRHRMTVHDMQEFELAYAPPFSSAKDPVNMAGYVGS
                     NVLNGDVKTAYWYEAMNADISRTFLLDVREPSEYQKGHIPNAVNIPLDQIRSRLKEIP
                     RDKEIIVNCQVGLRSYIGARIMMQNGFDNVRNLSGGYKTYSAVEADMKSI"
     gene            10564..11268
                     /locus_tag="PKU88_11265"
     CDS             10564..11268
                     /locus_tag="PKU88_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPX69887.1"
                     /translation="MSNISLILAFSAGLLSFLSPCVLPLVPAYITYITGSAIADLNTG
                     RARLYALYKSLGFVLGFSLVFIAMGASITSIGRLFAGNMNTFRKIGGTLIVLFGIHIT
                     GLFKIKTLYYEKRLVPFEKLSRNISSVFIGMAFAAGWTPCVGPILASILIYAGSMETV
                     TMGILLLAAYSLGLAVPFILTAYAVGSFSGYFKRISGYLNTISIISGVLLILMGILIF
                     TNKIAALSRYFDFFNL"
BASE COUNT         2873 a         2836 c         1850 g         3715 t
ORIGIN      
        1 atattatcgg cagctcgttc agctaaagtc ttcctgctgt atttacgggt ttcttctttt
       61 cttttaattc tttccctctc ttttcgctct gcctccaact gcttctgcct ctctttttct
      121 tccttttcca actgtttctg ccgttctgta tattccttct cctctttttc agccatctgc
      181 tcccgcattg cctttgcctg cagcatctcg taagcagatt ccctgtccac ttcggtatcg
      241 tacttgctgc tcatggatga agaattgatt atccgaaggc gagtaaaatc atcaatcgtt
      301 ccgaatttac tctggggagg aagaatgaat gcacgctcca ccatacccgg aatccctttg
      361 gaatccaggc atgacaccaa tgcttcccct gtgcccagct ctgttatggc agtttccgta
      421 tcgaaggcag gattcgtcct gaaggtctcc gctgctgctt ttacggtctt ctgctccgcc
      481 ggcgtataag cacgaagggc atgctgtaca cggttaccca gctgtcccag cacttcttga
      541 ggtaaatcca taggattctg tgttataaaa tatactccga cccccttgga tcttatcaac
      601 cttaccacct gctcaatctt ttgaagcagt gcctttggtg tatcatcaaa caataaatgg
      661 gcttcatcaa agaaaaatac cagcttgggc ttactcacat ctcctgtttc aggtaaagtt
      721 tcaaaaagct ccgacagcat ccacagcaaa aatgtggaat acaatgcagg gctgtggaaa
      781 agctccacgc aatgcaatat attgatatat ccctgtcctt cggaatcggt cctcatccag
      841 tcataaatat ccagagcagg ctctccaaag aactgtgctc ctccctgatc ttccaggaca
      901 agcaggctgc gttgaattgc tcctatactt tgagaggaga cattcccata tttcaaggta
      961 tactcctttg cgttttcgcc cacatagctg agcatagccc gaagatcctt catgtccaat
     1021 aggagcatgc cgttctcatc cgctacgcgg aatactatgt tcagcacccc tgcttgtgta
     1081 tcattcaggc ccagcagccg ggacagcagc aaagccccca tctcagaaac ggtagtgcgc
     1141 acaggatggc cgcccttgcc aaatacatcc caataacata cggggtacga tttatagttg
     1201 aagccctcta atcccaactt agctattctt tctgccatat tgggattatc gctgccctgc
     1261 tccgacagtc cggcaaggtc tcccttgata tcggtaagaa agacaggcac tcccatatcg
     1321 ctgaaagact ctgccatcac tttgagagta atggtttttc ctgtaccggt tgctcctgct
     1381 atcaagccgt gtcgattcgc catattgggc aggaggtaaa caggattatc ccctttggct
     1441 atccatactt tattatcaaa gtacataaat gccgctcctt tcactgtatt ttcaaaaaaa
     1501 tgcgtctcgc gacgcttttt tttcgctagg gaaccctatg gttcccttcg acggatgcaa
     1561 gcatcctgag caccctcctt tatcggcagg gggcttcacc cccttgcatc ccccattgta
     1621 tagaaaagta tattttccta tacaataaaa aagcaccttc tgtcaatata ctaacagaag
     1681 gtgcctcggt tgtaaatata taggctcttg gcagctaatc tattcggctc tttttaaagc
     1741 gcctaccaca ctggttacaa tcaatattgc aataattgtc tccggtattc cgttcgcaat
     1801 gcctatgccc ataattattt tccttgccaa tgcagggtct tgtcctatac tctttacaaa
     1861 gctttccgca taaatcaaat aaatcatgct caaaaccagc actgtattgg tcattgttcc
     1921 tacaatagcc gatattacaa gatccagtgc tttgccccta aacttcctgt ttgccaaaaa
     1981 tgctatcgct gctgtaaaag ctatcagcgc aatattcaca gctattaacc aggggctttg
     2041 agaattttca atatttttgt aaataccata gcttaggtag ccgattattg ctatccagat
     2101 gacattaagg atacctaagg tttttctgtt tccgagattg ctcaaagcag tatatacata
     2161 ataggatgta ataccgataa gcacccttgg cactatggaa accaatggat taaggaaaac
     2221 aaaggatacc ggtgtgggat tggttaatgc ctggaaaaca ctgaagagtc caaatataag
     2281 ccctacaagt gcgccaacta tcgggccttc cataatagca cctattatta caggaatgtg
     2341 cattatagtt gcccttgtcg gtcctacggg tatgaatccc aggggcgtca tgcccagaat
     2401 tgcggataca cctcccaata ccccgacaat aactaatttt cttacgtcaa atctctttac
     2461 tgcttccatt ttaatacctc cgctctagtt ccatattttg gataccagtc gtaaaacata
     2521 atgtttataa ctatatccgg tccaactcct tacggatgct ggcaacaaga atattataat
     2581 aaaaataata aaaaaaaaca ataagttaat tttaaaaacg atttggaggg tataaacggt
     2641 atactatata tcacagcagg caatcagccc atggcatgac tttgcatata caactgcatt
     2701 ggttactgcc cgtgaaaccg cctcggcggc catagtgccc acaaggttga tatccgcttt
     2761 tatctcgttg gtagccatag taaatattgt atctccatcc atcatggtat gcaccggatt
     2821 gattgatctg gcaaaaccgt tatgcgccat agccgcaatt ttatttgctt cagctttggt
     2881 taatacggca tttgcggcaa ccactcctat ggttgtattc ctcatgggaa agcccaacac
     2941 ccttccttct cttttcatta tttcatatgt attcagcatt tccctttttt catagtcata
     3001 tacccctgcc agctgcctgc cggtttcata gtcaaaaata tctccaaagc tgtttgccga
     3061 tactattgca gacactatca gttcccctga cttgagagag gcgcttccca gcccggattt
     3121 catggcatgc tcggggccaa aggctttacc tacggtagca cccaatccgc agccggcatt
     3181 tccctgtctg ccttcttccg cagatgcact tcttgccgca tcgtaaccca tattcaaatc
     3241 cggtctgatt ctgtggtcac ctatgttcag gtcaaaaata actgcagaag ccacaatagg
     3301 cactttagcg accccgacat caaatccgat tccctgctct tccaaatacc tcatcacccc
     3361 ggaagctgct tcaaggccga aagcacttcc tcctgacagt actactgcat gaatcctgtc
     3421 caccagcttg tccggcttca ataagtccgt ttccctggtt ccaggagcag agcctctcac
     3481 gtccactccc cctacggcac ccttctcaca gataaccgcc gtacaaccgg tcatgccttt
     3541 ctcggattga ctgtgtccta ctataatacc ctttacatcc gttatatatc ccggatacat
     3601 acctatccct ccctcgattc caatggtgcc aggcaccaaa caaaagaact taatttcttt
     3661 tcaataagtt tcttataccc gtatctgaca tgtctatgtt gtctcctatc tctttatatg
     3721 aatatttatg acttaaagct tcttgggcat atttaacttt gtaatctgtt aaatccatcc
     3781 gccgtttccc agctttaata agccaatatc cttcttcact cacacctgtt cctattaata
     3841 tttcgtccaa tcttttcctt tcctttacct gttttcttgt caggcacatt atctggtacg
     3901 cctcttctcc tattgcttta ctgttatcat agtcaatctc ttcatcctgc tccatgtact
     3961 ctttgtattt tttgattgct tctttcctgt cctttgacag catgtccata atcatttcaa
     4021 tatgtatata gctgtttatg ctatttctat aaaatacatc actgctccat ttatactttt
     4081 caacgctatc acagatgtgc gcctttattg gattttgatg tatgtatctg agaagagata
     4141 acatatacct ttcatcttgt accaatacag ctttgtatct tccctggaaa acgtgtccta
     4201 ccctctcata tttgtaattg aagtacttac tatacttgtt attaatctgg tgcattatct
     4261 cctgcagctt cttattatac gtctggatta taatatggta atgattatcc atcaagacat
     4321 acccatatat cctgtactcc attcctacgc tataatcttt caactgtttc aaaaaataac
     4381 ctttatcaat actttctctg aaaatgtact ctttattatt tcctcttgct attacgtggt
     4441 atattccgcc cttatattct tctctccacg gcctgcccat aaaaaagcac ctcctaatat
     4501 aatactatca gaaggtgttc catatgtaaa tatctgcctt attttgttct tttgttcggt
     4561 gcctggcacc tatgtatatt caattaatcc gaagcttatc tgaattgctg tatccacctg
     4621 tttcataagc tcctccgaca aatggccgat tttttccttc agccttctct tgtctattgt
     4681 ccgtatttgt tccaataata tgaccgagtc ctttgttatg ccgtatttca aagcttccag
     4741 ctcaacatgg gtgggcagct ttgctttatt aatctgtgaa gttattgcag caacaataac
     4801 agtgggacta tatttgtttc ctatatcatt ctgtattatc aatacgggtc ttacaccgcc
     4861 ctgttccgat cctatgaccg ggcttaaatc tccatagaaa atatcccctc ttcttaaaac
     4921 cacattattc acactccgca agccttgttt catatcttat tattgtttca tagtctgccg
     4981 taagtcctat ttctgctagt gttacattta tttctgacat ttcaagatat ccgttcctta
     5041 acttttctac tgcattagac ctctttatct cacttatata aaaacgcatg gcgtctctga
     5101 tgaattcact tctattaata catttatgac ataccatatc atcaatttct tttaaaagcg
     5161 tgtccggcat gcaaacggca atctttttta aatcagccac actagcacct cctaagtttt
     5221 ttgaagacca cacagagtat acaacattct gcatcgtcaa taatatgtat gagcaaggag
     5281 tgtaaaatta tgtgtcggaa atgcacatta taccatatat gattatacta accgcatata
     5341 caatattata taccaattct tgcactatgc ctatgtacaa tatatggcag catctagtct
     5401 actaaataat tcaacacatc gactatcttt ccgtccttga catatactct cggaactctc
     5461 ctgcttatca tgcatacaat ttcataattt atggtatcaa gcttttctgc aagttcatca
     5521 atatgaataa agccttctcc ctgtctcccg aaaagcaccg cctcatcacc gacttttaca
     5581 tcctcgatac ctgttacatc aaccatgcat tggtccatgc atattcttcc aaccacaggt
     5641 actctccggc cttttaccaa tgcctcagct ttgcctgaca gcattcttgt atagccatcg
     5701 gcatatccga taggcagcga cgcgattttg ctcttttttt ctgcaatgaa ccttcttccg
     5761 taacttacgc tctgtccggc ttctatctcc ttgacatgtg agattctgac cttcaatgac
     5821 ataacctgtt taagctcaat cctatcaagc attacctcat ccgaaggctt caggccatag
     5881 agaattattc ccgccctgac catattcaaa tgcatttcag gcaggtcaat tattgtagca
     5941 ctgtttccgc agtgttttac atttattctg acattctccc tttcaagctc cttgcatatc
     6001 cacgtgaatt tcgcatattg ttcttcagta tatgctttat ccaaggcatc agccaccgca
     6061 aaatgtgtaa atatgccttc aataacaata ttgggcagtt gactaatctt ctttataagc
     6121 tgtacggagt ccttgtcggg ctttaagcct atccttccca ttccggtatc aacttttata
     6181 tggatttttg ctgttttatt ctgcttcaca gccgcctttg aaatagcttc cgccagttcc
     6241 catgaataca cagcctgctc caaattgaag cttactattt cctctgccct ttccttgtcg
     6301 gtaaagccga ggattagaat aggcactcca aagcctgcat tccgtagttc gataccctca
     6361 tccaatacag caatggcaaa tctgtctgct ccattgtcca aaagggtttg tgctattttt
     6421 gttgctccat gtccgtatcc gtcagcttta attactgcgg ttacaagtgc atctttccta
     6481 gcaagcctcc ttatttccct catattgtgg gcaagagcat ccaaatccac ttccgcccat
     6541 gcggccctga tttccttcag atcgtacatt tcttttcatc tcctgtaatt tcatagaatt
     6601 ttttcgtatg atttcaaaat ccccgtcttc caatctggga ttgtagacaa aattatcata
     6661 aaatatctct acgtatgttt tgccgtcact tccgtatatt tccgctctta ggggcacaat
     6721 ttccgacctg tctatccaaa gcttttcaaa atgcatgtat ctgttcggct caggaatctc
     6781 aaattctgca acaatgcact ccttatcccc atacttttca tagctgactt ttatttcatc
     6841 ggtttttctg atatgttcca taaatgaacc tatgaagctg ttatactcca ctgaccctga
     6901 gaaatcttca gttacaaaat actgatttat tccgggatga tatatataag agctgtgtcc
     6961 gttatatata gtcacttgtc cttgaagctc cctcggtgcc aaaacttcta ccctgtactt
     7021 gtccggactt ttataggaat gcttcagcct gtattccatt gtacttctgt tgtttgtgac
     7081 cttcataaca acatcgcagg tataggcctc cagcttcaag agcctcctgc cggcttcccc
     7141 gaagttcttt ttctgcttta tcattccgca gccggttaaa cctgcacata caataatgag
     7201 cacgcaaata atcctacgat acacaatata ccctcctgtt actccatacc gtattattta
     7261 tacattattg tatgtgaaat ttttttatta atgaatattc tttcttgcct cttcatggct
     7321 ttgaaaaaat aaatcctcaa taaccatgcc ttccctttcc atggaaactt ccgccactgc
     7381 ggtagctgta gcaaattccc tggaatggga tatggttaca tgtattatcc cgattccgtt
     7441 atcctctata aactgtttga cattgccctc aaaacggact atgggctttc cgaccgggcc
     7501 tctcaggatt tcgatatcct tccatctaaa acctcttaat cccgttccca gcgccttaag
     7561 cacagcttcc tttgcagaaa aacctcccgc aatatgctgt gctttcatat cttttttctt
     7621 gtaaaattcc agctcctgtg cggtaaacaa tctttcaata aatctcgggc ttctggatat
     7681 tgccttctta attcttttta tttcaacaat atcagtccca ataccgaata tcattatatc
     7741 actcccgtat ttcagcacat tcataatgca ctttaattgt atatattcta caataaaatc
     7801 ctatatcctc tcattaaccg tatataataa taaaaaaaca aaggtcaatt ccgccgctgc
     7861 agcagaatca accctatatg ctcctatggc aaaaagcttt catccaggct ttccatctcg
     7921 tccgctccaa tcattccatg aataaataaa tacagcttgt ttaccctttc ctccatatcc
     7981 gcctttttct gtatcaattc cgccagcttc tgtctgatat cgttcacctg ctcctggcac
     8041 ttctccgact ccgaacggct ctcatttatt agctcatagg ccctcttgat agtttccttc
     8101 agaagctgcg tattcatatc atttatttgt ttagggatag attccagttc ttctaaaagt
     8161 accgaaatct tcctgttaat ttcttttatt tctttctccg cttcctccat tttggataag
     8221 gcggtcttgt cgttattttc atttatatcc tttgaaagat atagtatttt attcataacc
     8281 attttttttc ttttttccag attatataac tcggaatcac attccttatt cctggcaata
     8341 agcgcttcca atctggctat aattttttgc atgcttctgg acttgtttcc ggaaaatagc
     8401 tgtatccact ccggtacgta gatgagtatc ggaattttat ttcttcttac aatattgtct
     8461 ttaaaactga tgtctttgcc cataggtatt ctctccattt cgtacagcat atccatccgc
     8521 atatcatgaa gaacactata aattgccaaa aattatttta ttaaattaat tttactataa
     8581 aaacaaatat agtcaaatgc ttttcatatc tgcttccact gcgctgtatg ttttatagcc
     8641 gccgctcaga tttcttacat tatcgaaacc gttttgcatc attatccttg ctcctatata
     8701 agaacgcaag cccacctggc aattgacaat aatctctttg tccctgggta tctccttgag
     8761 cctgcttctt atttgatcca ggggaatatt caccgcattt gggatatgcc ccttctgata
     8821 ttcggaaggt tcccgtacat ccaaaagaaa tgtccggctt atatccgcat tcattgcctc
     8881 ataccagtaa gccgtcttga catccccgtt caaaacattg cttccgacat aaccggccat
     8941 gtttacagga tcctttgcgg atgagaaggg cggagcataa gcaagctcaa attcctgcat
     9001 gtcatgcact gtcatcctat gccggattgc tattgctata taatctattc tcttatccac
     9061 cccgtcagaa ccgactatct gtgcccccag cactctgcct tcaggagtat agatgatttt
     9121 taccgccatt aattttcctc ccggataata gcccgcatgg gaagcagagt gtgtagtagt
     9181 tgtgatatag tcctttccca attcctttcc aagcttttta agggccttct cattaactcc
     9241 cgttgaagct gcaacaatat caaatacttt tgcgattgcc gttccctgtg tccccttata
     9301 gctttccctg cgtcctgcaa tattgtttgc cactattctt ccctgtttgt ttgcaggccc
     9361 ggcaagagct atcagcgccg gtttgccgct tacataatct gttacttcta tagcatcacc
     9421 cagagcatat atatttggat cagaggtctg gagatactca tttaccttta taccaccggt
     9481 tacacctatt tcaagtccag cctccttagc cagggagtta tttggtctta ctccttttga
     9541 aagtattatc atatctgccg gtatccgctt tccgctctgc agtactgcaa ctgtcttatc
     9601 gccctccggc tcaaaggcct ttaccccatc ctttatgtga agtccgcagc ccttgcttct
     9661 tatatgatta tgaactatgg cagccataga ataatccagc tgcgacataa cttgttcatg
     9721 ggctactact atgtcaactt ttaaaccggc tgaatgaagg ctctccgcca tctcgagacc
     9781 aataaaacct cctcctgcga taactgcgtt cttaggtttt ttctcattta aaaattcttt
     9841 tatggaatca gtatcaggaa tatccctcag ggtaaatatg ttgcggctgt caactcccgg
     9901 aataggaggc ttgaccggaa cagcaccagg ggagagtacc agaacatcat agttttcttc
     9961 ataattcctt ccatccttta gattctttac ttcaactacc tttctctgcc ggtctatcct
    10021 tttaacctct gaaagttctc tgacatctat attaaagcga gcctccatag cctctgctgt
    10081 ctgaaccagc agtttctttc tttcctttat tgttccgccg atataataag gaaggccaca
    10141 attggcgaag gatatgtact ggcccttttc aaatagtatt atctcagccc tttcatcaat
    10201 cctcctgagc cttgctgcag tggatgctcc acctgcaacg cctcccacta taactacctt
    10261 cttactcata tccattcctc ccctgtcttt tgtattggtt atgtattcat ttcaacatta
    10321 tttatatatt tgttatccct atattatgta aaggttcata atgattatat ttttatatcc
    10381 ttcagctgtc aagaatccat gtaatcggct attgagtcaa aatccatata aatactttac
    10441 attatcggca ttatataata taataaatat atacccatgg ggggtatgat tttgattcga
    10501 actttggata acagacacta aagtgtaaca agtaataaga tttaatcaag gcaggtgatt
    10561 aaattgagca acatatcact tatactggct ttttcggcag ggcttctgtc ctttctttcc
    10621 ccatgcgtgc tgcctctggt acccgcctat ataacttata ttaccgggtc tgctattgct
    10681 gatcttaata ccggaagggc aagactttat gccctgtata aatccctggg gtttgttctg
    10741 ggcttttctc tggtattcat agcaatggga gcctcaataa cctccatagg caggcttttt
    10801 gccggaaaca tgaatacttt caggaaaatc ggcggtactc tgatagtact gtttggaata
    10861 cacattacag ggctttttaa aataaagaca ttgtattacg aaaaaaggct tgtgcctttt
    10921 gaaaagctga gcagaaatat aagctcggta tttataggaa tggccttcgc agccggctgg
    10981 actccctgcg taggccctat actggcatcg attctcatat atgccggcag catggaaaca
    11041 gtaaccatgg ggatactgct gcttgcggca tattctttag ggctggcggt accatttata
    11101 ctaactgctt atgctgtcgg aagcttcagc gggtatttca agagaatatc agggtacttg
    11161 aataccatat cgatcataag cggagttttg ctcatattaa tgggtatact catatttaca
    11221 aacaaaattg cggcgctaag ccggtatttt gattttttta atctttaatc agag
//
LOCUS       DAOOAB010000066        11233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_7372_length_11233_cov_16.155395, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000066 DAOOAB010000000
VERSION     DAOOAB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7372_length_11233_cov_16.155395"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..1551)
                     /gene="hemW"
                     /locus_tag="PKU88_11270"
     CDS             complement(415..1551)
                     /gene="hemW"
                     /locus_tag="PKU88_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPX69888.1"
                     /translation="MGEKMKEVGLYIHIPFCRSKCLYCDFNSYPCREDDAPEYTEALL
                     RELHAYQERYNFVYKTVFIGGGTPTVINCRLIGEIMDKIAPRIKAGAEISMESNPGTV
                     TFDTLKYYRSLGIKRLSIGLQAWQAELLGELGRIHTTEDFLNAYNWARESGFDNINTD
                     LIFALPNQTTDMWKETLDKVVSLGVDHISCYSLILEEGTGLYRLYKEGKLQLPDEELD
                     REMYRIAVDTLGSNGYLQYEISNFARKNMECRHNLVYWRNEEYLGVGAGSHSKLEGKR
                     FWNYKDINRYISLAAEGKLPVEGQEELSADEDMWETIFLALRLNEGLDMAGFNSKYGV
                     DFRTRYGSQLKKLAAQGLVFIESDRLKLTKKGIDLSNSVFVEFA"
     gene            complement(1554..3362)
                     /gene="lepA"
                     /locus_tag="PKU88_11275"
     CDS             complement(1554..3362)
                     /gene="lepA"
                     /locus_tag="PKU88_11275"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPX69889.1"
                     /translation="MAGDRQKHIRNFCIIAHIDHGKSTLADRLIEKTGVISARDMEEQ
                     VLDNMALERERGITIKLQSVKLEYTAQDGQEYVLNLIDTPGHVDFTYEVSRSLAACEG
                     AVLVVDATQGIEAQTLANVYLALDNNLELYPVINKIDLPSADPEFVKKEIEDIIGLDA
                     SDAALISAKEGLNIEEVLEGIVKYIPHPAGDEDKPLKALIFDSFYDSYKGVIAYIRVV
                     DGVLKPGMNMRMMSNNKEYEVVEAGTLKPWLNPTGLLMAGDVGYVTASIKNVKDVHVG
                     DTITDADNPCKEALPGYRKVTSMVYCGIYPADGADYENLKDALEKLQLNDAALLFEPE
                     TSVALGFGFRCGFLGLLHMEIIQERLEREYNLDLVTTAPSVIYHITKTNGEKLEVSNP
                     TNLPVPQEIEYMEEPIVKATIMTPTEYVGAIMELCQNRRGTFRDMQYMEEKRAILNYD
                     LPLNEIIYDFFDALKSRTKGYASFDYELKGYERSDLVKLDILLNTEAVDALSFIVHKD
                     KAYARGRSMAEKLKEVIPRQMFEIPIQAAIGTKIIARETVKALRKDVLAKCYGGDISR
                     KKKLLEKQKEGKRRMRQVGSVELPQEAFMSVLKLDD"
     gene            complement(3635..3967)
                     /locus_tag="PKU88_11280"
     CDS             complement(3635..3967)
                     /locus_tag="PKU88_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69890.1"
                     /translation="MSKRVDKKKQRMEALKNKLKKALGIFFCIILLILSVSIADMSAR
                     RMMMCNDDKYAIAVSLQEDSLLRVDIAGEKLMINIEPAVRIFNYIYDNSTRHCDSIVK
                     SIKAKLNE"
     gene            complement(3974..5176)
                     /locus_tag="PKU88_11285"
     CDS             complement(3974..5176)
                     /locus_tag="PKU88_11285"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HPX69891.1"
                     /translation="MNKKWLFALFLALAVAASLLFLKWLYSPDPYLSKYNISSTGSAE
                     VFNYDIKKRLKLEEYSNNFYIQLLNTVMTGAKANYEIEYGCPMPGLLESAASSITDGL
                     HTGAGNPITYLNFVFTAFSQYDPSLFSYAETGSSAFPNITDFENAPQGAIYFSEEDEY
                     KAEIPAETPASQIVEEAVAIPGKIALNSKSPQILVYHSHSTESYMPNTAGNYHTQNDK
                     YNVIAVGSIFAKELQDKYKYKVLHDKTKHDKESYAYSYANSLQTIKNSINKYNSIKVI
                     LDLHRDAFDAEKATFAEKTAKKSEYTTSINGKRAARIMLVIAKENPNYAELEKFAVYI
                     KKKMDKLYPGLFYKIDIKSRGKYNLYFSNHSMLVEVGCMLNTIEEAQYSAELFARVMG
                     EVINDLKE"
     gene            complement(5286..6263)
                     /gene="gpr"
                     /locus_tag="PKU88_11290"
     CDS             complement(5286..6263)
                     /gene="gpr"
                     /locus_tag="PKU88_11290"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HPX69892.1"
                     /translation="MAGIRTDLAIEAREFYNKQENRQDIPGVVVDVDKDESYTVTRVK
                     VVEDVGARIIGKPKGNYITIEVPKLRENDRELQEEVGKVLAEEVARLVSLNDQSVIMV
                     VGLGNWNVTPDALGPRVVEHLLVTRHIKEYVPDQIDEGVRSVCAIAPGVLGITGIETS
                     EIIRGIVDKVNPDLVIAIDALASRKMDRISTTIQIADTGINPGSGVGNNRKELSRESM
                     GVPVIAIGVPTVVDAATMASDTIDMVIDTLINESPESGEFYNMLKSMDKTEKHKLIDE
                     VISPYIGNLMVTPKEIDRLIDDIAIVIANGLNIALHPNVDQDEMNRYLN"
     gene            6499..6762
                     /gene="rpsT"
                     /locus_tag="PKU88_11295"
     CDS             6499..6762
                     /gene="rpsT"
                     /locus_tag="PKU88_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPX69893.1"
                     /translation="MANIKSALKRIKVANFKTRRNRIIVSSLKTSIRRFEDSIKNGNM
                     SEAKELYRKVASLIDKAVSKGTLHKNTAARKKSRLSKKIKDIA"
     gene            complement(6829..7911)
                     /gene="holA"
                     /locus_tag="PKU88_11300"
     CDS             complement(6829..7911)
                     /gene="holA"
                     /locus_tag="PKU88_11300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPX69894.1"
                     /translation="MGYRQLMNDIKNNCIKPVYIIFGNETYLIDKGREALKKAVVTSF
                     PELNYTRLEGEKLEADELSAACRTFPFGTEKRLVEVRNPEILRSKGRGREEDEDEEEN
                     EDEKASAPGKDAQPFIEVLQNLDDTVCLLLLSYGSINKKRKKLLDEVKKHGAVLEFNR
                     VERDELAQWIKKTLGKSGKNIGPRELNHFISSTGYLDKNGSKTLYDLENEIKKLTGFM
                     GNSTEVTIEHIEAIVPRNIENDIFKLINACYEKDTGRSLRIFNDLLLEGETTLGVLAL
                     LSSQVKNMLKVYELYEKKYNRRGISEKLKLHEYTVKLCIQYGSSIKRQALQSAFRKCL
                     DTDLSIKSGGMGDRLAMEMLLAGLFE"
     gene            complement(7974..10289)
                     /locus_tag="PKU88_11305"
     CDS             complement(7974..10289)
                     /locus_tag="PKU88_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPX69895.1"
                     /translation="MASFAFSFFFGILTASIILPDIPIILGGFLLSGAAVLINRKNKP
                     NLKAVFMLLVFLVGMADYSLAYRAESRLDAFDGKTAVYECLITDNPVERGKYLQYPAR
                     SISVQYEGKHYNFSEKVFLKIDADTVFKFGDKLSIRGQCSDIAGIRNPGDFDFKLYYK
                     NKGIAKQIAADRAALLKENSAGVFKAMLYYSKEKVRSIINEALPQEEAAVLTGIITGD
                     KADMDEDMKEAYMKTGLSHILSVSGLHVGFLMMLLTYLLMPFKLDKRLQGAVTLLITV
                     YYVLLIGAPFPSVRAVIMLAVLMAGKAAGREYDLLASVSFAFMIMLVFKPLAIHDTGF
                     MISFAAMYSIALLYPAVYGILRCMPGVIRNPAALSMSVWLGLAPVLAHYFNYISIISI
                     IINIAAIPLSFIITVTGFIGVFAGIVSKTLALYIFSVDYCFINLLSFMIRKAAELPAA
                     GFYIPRLPIYIHALYYGGIGLFIGFCKLAFFRVYMRRLALSYLLAAVIAISVYNLPSE
                     KLRMVFFDVGQGDSCCIITPQKKAVLIDGGGSSRNGDYYYDVGGKITFPALLHQGIWS
                     IDTVIVSHLHDDHMEGLLKVMEGYKIKNIILPRVSAGTDEISGSSGALLDICRNKGIK
                     IHRLGSGDQINLGGGVKIDFLHPGKAAKADENENSLVGVLYYGDFSALFTGDIGKETE
                     GLLQAEALKSVVMKVPHHGSGRSSSEEFLEKVRPRVSVISVGSNNYGHPSPDTLKRLA
                     GIGSLVYRTDGNGGVTIITDGESMKVKTVKQ"
     gene            complement(10425..10808)
                     /locus_tag="PKU88_11310"
     CDS             complement(10425..10808)
                     /locus_tag="PKU88_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPX69896.1"
                     /translation="MGNNTPKNDQQRRSTGTGVIGFIVRFVVAAIVLMVTAYFVPGFR
                     MRGGFTTAIIAALVIAAADFLIEKLFKFDASPLGRGISGFLVSAAVIYFTQYLVQGME
                     VSLLGAVIGALVIGLVDTVLPTRFM"
     gene            complement(10876..11142)
                     /locus_tag="PKU88_11315"
     CDS             complement(10876..11142)
                     /locus_tag="PKU88_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69897.1"
                     /translation="MNISETLVVPIIVSLTQLAKGLGLPKKFCALAAVVIGVIIGVFF
                     LEPLCIKAGVFKGIVYGLSASGLYSGTKNTIEQVNGKKVKKKKK"
BASE COUNT         2956 a         2789 c         2041 g         3447 t
ORIGIN      
        1 caacctcctg ttggtatgtg ctggatttac ctcacgtaaa tcccacggat tttaccgcgt
       61 atcttctctc gattctgttg ccaaagccaa taagtgctcg ctgttataca aggctagtgc
      121 tctgtaggat gactacaaac ttcaaatgtg tcgcattatt caaaccaccc accaattgcc
      181 cgaagaatgc ggcgcgatta cactataaca tttatatatg ttatatagtt gcataagaag
      241 gtttcgatac ttacatcatt atcgataatt acctatttac cggaatattt atcccgccgt
      301 gcatttagcg gtttttcgtt ttttcagtgt taccatagct cccaaagccc tgccctaaac
      361 ctctaacctc gcgcctcgca cctcgtaccc gaagaatgtg ttgccttctt ctccttatgc
      421 gaattccaca aaaacagaat tagataggtc tatccctttc tttgtcagct tcagcctgtc
      481 cgattcaata aacacaaggc cttgtgccgc aagcttctta agctggctgc cgtatcgcgt
      541 tctgaagtca accccgtatt tgctgttaaa gcccgccata tccaaccctt cattgagccg
      601 cagtgccaga aatatcgttt cccacatgtc ctcatcggcg gataattcct cctgcccttc
      661 caccggcagt ttcccctcag cggccaggct gatataccgg ttaatgtctt tataattcca
      721 gaatcttttc ccctccagct tggagtggct tccggctccg acgccaagat attcctcatt
      781 tctccaatac acaagattat gcctgcattc catgttcttc ctggcaaagt tggatatctc
      841 atattggagg tagccgttgg agcccaatgt atccactgcg attctgtaca tttccctgtc
      901 caactcctcg tctggcaact gcaatttgcc ttctttgtac aacctgtata atcctgtgcc
      961 ctcttccagt ataaggctgt agcaggaaat atgatccacg cctaagctta cgaccttatc
     1021 cagggtttct ttccacatat cggtggtctg attgggaagt gcaaaaatta aatcggtatt
     1081 tatattgtca aatccggatt ctcttgccca attataggca ttcagaaaat cctcagtagt
     1141 gtgaatcctt cccagctcac ccagcagttc tgcctgccat gcctgcaggc ctatgctgag
     1201 cctttttatc ccaaggcttc tatagtactt cagagtatca aaggttacag ttcccggatt
     1261 ggattccatg ctgatctccg ctcctgcttt tatccggggt gcaatcttat ccattatttc
     1321 acctataagc ctgcaattaa taacggtggg agttccccca ccgatgaata ctgtcttgta
     1381 aacaaaattg tacctttcct gatatgcatg aagctccctg agaagcgctt ctgtatactc
     1441 cggcgcatca tcttccctgc acgggtagga attaaaatcg cagtacaggc acttgctcct
     1501 gcagaaaggt atatggatgt acaaacccac ttccttcatt ttttcaccca cttttaatca
     1561 tccagtttca ggaccgacat gaaggcttcc tggggcagct ccacgctgcc aacctgcctc
     1621 attctcctct ttccttcctt ttgcttttca agaagctttt tctttcttga tatatctccg
     1681 ccatagcact tggcaagcac atccttacgc aatgccttta cagtttccct ggcaataatt
     1741 tttgttccga tggccgcttg tatgggaatc tcaaacatct gtctcggaat tacctccttg
     1801 agcttttcag ccatagacct tcccctggca tatgccttat ctttatgtac tatgaaggaa
     1861 agtgcatcaa ccgcttctgt attaagcagt atatccagct taacaagatc tgacctttca
     1921 tatcccttca gctcatagtc aaaggaagca tagccctttg tcctggattt taatgcgtca
     1981 aaaaagtcat aaattatctc attcagcggt aaatcataat taagtatggc tctcttttcc
     2041 tccatatact gcatatccct gaaggtgccc ctgcggttct ggcaaagctc cataatcgct
     2101 cctacatatt ctgtaggtgt cattatagtg gccttcacaa tgggttcctc catatattct
     2161 atctcttggg gaactggcag gtttgtcgga ttggaaacct caagcttttc tccgttggtc
     2221 ttggtaatat gatatataac acttggtgcg gtggtaacaa ggtcaagatt atattccctt
     2281 tccagcctct cctggattat ttccatgtgc agcaatccca ggaagccgca tctgaagcca
     2341 aagcccagtg ctaccgatgt ctccggttca aaaagcagtg cagcatcatt aagctgcagc
     2401 ttttccagag catctttaag gttctcgtaa tctgctccgt ctgctggata aataccgcag
     2461 tataccattg aagtaacctt cctatagccc ggtagtgctt ccttgcaggg gttatccgca
     2521 tcggtaatgg tatctcccac atggacatcc tttacattct ttatactggc tgttacatag
     2581 cctacatctc ctgccataag caggccggta ggattcagcc agggcttcag tgtgcctgcc
     2641 tcaaccactt cgtactcctt gttgtttgac atcattcgca tattcatgcc cggcttcagt
     2701 accccgtcca ctactcttat ataggcaata acacctttat agctgtcata gaaggaatca
     2761 aaaatcaaag ctttcaaagg cttatcctca tctccggccg gatgaggtat atacttcact
     2821 attccttcaa gtacttcttc aatgttcagt ccttccttgg cagatatcaa tgcagcatct
     2881 gaagcatcaa ggccgattat atcttcgatt tcctttttta cgaactccgg gtctgcgctg
     2941 ggaagatcga ttttatttat tacaggatac agctccagat tattatcaag ggccagatat
     3001 acattagcca gtgtctgcgc ctcaataccc tgggtggcat ctaccacaag cacagcccct
     3061 tcgcaggcag ccaggctcct ggagacttca taggtaaaat ccacgtgtcc cggagtatct
     3121 ataagattca atacatattc ctgtccatcc tgagccgtat actccagctt gaccgattga
     3181 agctttatgg tgattcctct ctccctctca agggccatat tgtcaagtac ctgttcctcc
     3241 atatccctgg cggaaataac ccctgttttt tctataagcc tgtcggccag agtcgatttt
     3301 ccatgatcta tatgtgctat tatgcagaaa tttcttatat gcttttgcct gtcccctgcc
     3361 atatatggcg tcctcctttg tgccccttaa atctttaaga ttgtatcaaa tgagttttat
     3421 acatttacgc atattatagc atattagttt ttaacaataa atatatacta tatacaccgg
     3481 attccaaaaa atgaaatagg tttatgcaca acagaagaat aagaggacac gggggacggg
     3541 ggacggggac acggggacgt ttcccctgtc ccacttccag tgggacacgc gaaacgtccc
     3601 cgtgtcctcc ccatgtcctc tttataaaat aaaatcattc attaagtttt gcttttatgc
     3661 ttttcacaat actgtcacag tgcctcgtgg aattgtcgta aatataattg aaaatcctga
     3721 cggcaggttc tatatttatc ataagctttt ctccggcaat gtctaccctc agcaggctgt
     3781 cctcctgcaa agaaactgct attgcgtact tatcgtcatt gcacatcatc attcttctgg
     3841 ctgacatatc tgcaatgctg actgaaagaa tcagcagaat tatgcagaag aatatcccca
     3901 gggccttctt cagcttattt ttcaatgctt ccatcctctg ttttttttta tcaactctct
     3961 tgctcatctg catctattcc ttcaggtcgt ttattacttc ccccataacc cttgcaaaaa
     4021 gctctgcgga atattgggct tcctcaatag tattaagcat acatcctacc tctaccagca
     4081 tggagtgatt gctgaaatag aggttgtact tgcctctgct ttttatatcg attttgtaga
     4141 ataatccagg atatagttta tccatctttt tctttatata tactgcaaac ttttccagct
     4201 ctgcgtagtt tggattctct tttgctatta caagcattat ccttgcagcc cttttcccgt
     4261 ttattgaggt ggtgtattcg gattttttgg ctgttttctc agcaaatgtg gctttctccg
     4321 catcaaaggc atctctgtgc aaatccagga ttacttttat ggaattgtat ttattgatgc
     4381 tgttctttat agtttgaagg gaatttgcat aagaatatgc ataggactct ttatcatgct
     4441 tggtcttgtc atggagaacc ttatatttgt atttatcctg cagctctttg gcaaatatgc
     4501 ttcctacggc tataacattg tacttgtcat tctgggtatg ataattccct gcagtattag
     4561 gcatgtagct ttctgtggaa tgcgaatgat aaacgagtat ctgcggagat ttgctgttca
     4621 atgctatttt gccggggatg gcgacagctt cctcgacaat ttgtgatgcc ggggtttctg
     4681 cgggtatctc agctttatat tcatcttcct cgctgaaata aatagctccc tgaggcgcat
     4741 tttcaaaatc ggtaatattg ggaaatgcac tgctgccggt ttcggcatat gaaaacagcg
     4801 acggatcata ttgactgaaa gctgtaaaga caaaattcag gtaagttatg ggattgccgg
     4861 cgccggtatg aagcccgtcg gtgatgctgc ttgcagcaga ctcaagaagc ccgggcatag
     4921 ggcagccgta ttcaatctca taatttgcct tggctcctgt cattacagta tttaacagct
     4981 gtatgtagaa gttgttggag tattcttcaa gctttagcct ctttttaatg tcataattga
     5041 aaacctctgc agagccggtt gagctgatat tgtatttgct taaatatggg tcaggtgaat
     5101 ataaccattt cagaaataaa agcgatgctg caactgccag tgccagaaaa agcgcaaaca
     5161 gccatttctt gttcaaacac tccacctctt ttccgcccgt cttttgaacc cgattaaaaa
     5221 ttggacctgt ctgtatacaa atatatgcca tacactagaa aaaatgcgtc tcacgacgca
     5281 cttttttagt tcagatacct gttcatttca tcctgatcta cgttcgggtg cagtgcaata
     5341 ttcagcccgt tggctatcac tattgctata tcgtctataa gcctgtcaat ttcctttgga
     5401 gtgaccataa ggttacctat atacggggaa atcacctcgt ctatcagctt atgcttctct
     5461 gttttatcca tgctcttaag catattgtag aattctccgc tttcaggaga ttcgtttata
     5521 agagtgtcaa tcaccatatc tatggtatcg ctggccattg ttgcggcatc tacaactgtc
     5581 ggaactccga tggctatgac aggcaccccc atgctctccc ggctaagttc cttcctgttg
     5641 ttcccgactc cggatcccgg gtttatgcct gtatccgcaa tctggattgt tgtgcttatc
     5701 ctgtccattt tccttgaagc taaggcatca atggctatta ccaggtcagg attcacttta
     5761 tccactatgc cccttattat ttcactggtc tctatccctg taatcccaag caccccgggg
     5821 gcaatagcac aaactgacct tactccctcg tcgatctgat cgggaacgta ttccttaata
     5881 tggcgtgtta ccagcagatg ctccactacc ctcggcccca gtgcgtcggg agttacattc
     5941 cagtttccca gccctaccac cattatcacg gattggtcat tgaggctcac caatcttgct
     6001 acttcttccg caagaacctt tcccacttct tcctggagct ccctgtcatt ttccctcagt
     6061 ttgggcactt caatggtaat ataattccct ttgggctttc ctatgattct tgcaccgaca
     6121 tcttcaacaa ccttaaccct ggttacggta taactctcat ctttgtccac atccacaacc
     6181 actcccggga tatcttgcct gttttcctgc ttgttataaa attcccttgc ttcaatagct
     6241 aagtctgttc ttattccggc cacggcattc cctccaaaaa tctgtaaagt gtttttcatt
     6301 aattaataat tgaagtttgc gccttacctg atgtttttat acaaaatgca gttcttcata
     6361 attaatttta aagcgggtaa acttatataa ataatatata aaacatgccc aaaaaagctc
     6421 aatacaatgc acttgcattt ttttggctaa tatgatagaa taccttttgt caattgaatt
     6481 ttgaaggagg tgaagggttt ggcaaatata aaatcagctt taaaaagaat aaaagtggca
     6541 aatttcaaga caagaagaaa tagaataatt gtttcctcct tgaaaacttc aataagaagg
     6601 tttgaagatt ccattaagaa cggaaatatg agcgaagcta aagaattgta caggaaggtt
     6661 gctagtctta ttgacaaagc agtttccaaa ggtacacttc ataagaatac agcagcaagg
     6721 aaaaaatcca gattatcaaa aaagataaag gatatagctt agtatacgaa caaaaatttc
     6781 gggtgccgca aaacttttta atatgcagtt agcggcaccc tatttatatt actcaaataa
     6841 tcccgcaagc agcatctcca tcgccagccg gtctcccata cctcctgact ttatactcag
     6901 atcagtatcc aggcactttc tgaaagcaga ctgcagtgcc tgccttttta ttgatgaacc
     6961 gtactgtatg caaagcttca ccgtatattc atgaagctta agcttttccg atatacctct
     7021 cctgttatat tttttctcat aaagctcata tactttcagc atgttcttta cctgacttga
     7081 caggagagcc aatactccta aagtggtctc cccctcaagc agcaaatcat tgaatatcct
     7141 gaggctgcgg cctgtgtctt tttcatagca tgcattaata agcttgaata tgtcattttc
     7201 tatgttcctt ggaacaattg cttcaatatg ctctatagtg acctccgttg aatttcccat
     7261 aaaacctgtc agctttttta tttcattctc taaatcatac agggttttgc tgccattttt
     7321 gtcaagataa ccggtactgc tgatgaaatg attcagttct ctggggccga tattcttgcc
     7381 gctctttcca agggttttct ttatccactg agccagctca tccctttcta ctctgttgaa
     7441 ttccaaaacc gctccatgct ttttcacttc atcgagaagt ttttttcttt ttttattaat
     7501 acttccatag gaaagcagca aaaggcaaac ggtatcatca aggttttgca gcacttcaat
     7561 aaagggctgt gcatccttcc ccggagcaga agccttttca tcttcattct cttcctcatc
     7621 ctcatcttcc tccctgcccc tgcccttgct cctcagtatt tcaggatttc tcacctctac
     7681 taatctcttt tcagttccaa agggaaaagt cctgcaagcg gctgatagct catcggcctc
     7741 aagcttctcc ccctccagcc gggtataatt aagctccgga aaggaggtca ccaccgcttt
     7801 tttcagggct tctctgcctt tgtctatgag ataagtctca ttgccgaata tgatgtatac
     7861 cggtttgatg cagttgtttt taatatcgtt cataagctgc ctgtagccca taatttcatc
     7921 accatgccgt ttcaacagta tttatttaat tataatatat aagctatcaa ctgtcactgc
     7981 ttcacagttt ttaccttcat actttctccg tctgttatta tggttacccc tccgttcccg
     8041 tccgttctgt atacaaggct tccgatgccg gccagtcttt tcagggtatc cggagaagga
     8101 tgcccataat tgttgctgcc taccgatatc acactgaccc ttggcctgac tttctcaaga
     8161 aattcttccg aggaggatct tccgctgccg tggtgaggca ccttcatcac aacggacttt
     8221 aaagcttcgg cttgaagcag gccttccgtc tcctttccta tgtcccctgt aaaaagtgca
     8281 ctgaaatctc cgtaatacag aacccccacc agagagtttt cattctcatc agcttttgca
     8341 gcttttccgg ggtgcaaaaa atcaatcttt actccacccc caaggtttat ttgatctccc
     8401 gagcccagcc tatggatttt tatccccttg tttctgcata tatcaagcag cgcaccgctg
     8461 cttcccgata tttcatccgt acccgccgaa acccggggca ggattatatt ttttatctta
     8521 taaccctcca taactttcag aagcccctcc atatggtcat catgcagatg ggatacgata
     8581 acggtatcga tactccatat tccctggtgc aaaagtgccg gaaaagttat cttccctcct
     8641 acgtcatagt aataatcccc attccttgaa gagcctcccc cgtcaatcag tactgccttt
     8701 ttctgaggtg taatgataca gcagctgtcc ccttgcccca catcaaagaa taccattctc
     8761 agtttttccg agggcaggtt gtagacagaa attgcaataa cagctgcaag cagatatgat
     8821 aaagcaagcc ggcgcatata taccctgaaa aaggccagct tgcaaaatcc tatgaacagt
     8881 ccgatacctc catagtacaa agcatgaata tatattggca gccttgggat gtaaaagcct
     8941 gcggcgggaa gctcggcagc ttttcgaatc atgaaggaaa gaaggttaat aaaacagtaa
     9001 tccacagaaa atatatacag tgccaaagtc tttgatacaa ttcccgcaaa cacccctata
     9061 aagcccgtaa ctgtaataat gaaggacaga ggtattgcag caatatttat tatgatgctt
     9121 ataatggaaa tatagttaaa atagtgtgcc agaacgggag caagcccaag ccataccgac
     9181 atggacaaag ccgccggatt ccttattacc ccaggcatgc accgcaatat gccgtaaact
     9241 gccggataaa gcagggcaat ggaatacatc gcggcaaagg atatcataaa ccccgtatca
     9301 tgtattgcca ggggtttaaa tacaagcatt atcataaatg caaaggatac tgacgccaaa
     9361 aggtcatact cccgccccgc cgccttgccg gccatcagga ctgcaagcat aataaccgcc
     9421 ctcacagagg gaaaaggggc tcctatcaaa agtacataat atactgttat taatagggtg
     9481 acagctcctt gaagcctctt atccagtttg aaaggcatta acaaatatgt aagaagcatc
     9541 atcagaaagc cgacatgaag gccggaaacc gaaagtatgt gggaaagccc tgttttcata
     9601 tatgcttctt tcatatcctc atccatatcg gccttatcac cggtaataat cccggtgagt
     9661 actgccgcct cctcctgagg caaggcttca ttaataatac tcctgacctt ctcctttgaa
     9721 taatacagca ttgccttgaa cactcctgca ctattctcct tcagcagggc cgctctgtcg
     9781 gctgctatct gcttggctat ccctttgttc ttataataca atttaaagtc gaagtctcca
     9841 ggattcctta ttccggctat atcggaacat tggccccgga tggacagctt atccccgaac
     9901 ttgaatacgg tatctgcatc aatcttcaag aaaaccttct cggagaaatt gtagtgcttt
     9961 ccctcatatt gaaccgaaat gcttctggcc ggatactgca gatatttccc cctttccacg
    10021 ggattatccg taatcaggca ttcatagact gcagtttttc cgtcaaatgc gtccagcctg
    10081 ctctctgctc ggtaggcaag actgtaatcc gccatgccca caagaaaaac taaaagcata
    10141 aatactgcct tgagatttgg tttgtttttt ctgtttataa gcactgccgc ccccgacaat
    10201 aaaaaaccac ccaatataat cggaatatcg ggtaaaataa tactcgcagt caatataccg
    10261 aaaaagaagg aaaaagcaaa gctcgccata acctcacgcc cgtttcaaaa ttttgtggat
    10321 ttgtataatt tctaccaaaa ctttgaatat ccttctatta tttacatttt ttactactcg
    10381 atataaaaaa ataaaatagc tgccattagc agctatttgc aatactacat aaatctggtg
    10441 ggcagcactg tatcgacaag gccgattacc aatgccccta ttactgcccc caataaactt
    10501 acttccattc cctgcaccag gtattgagta aaatatatta ccgccgccga aaccaggaag
    10561 cctgagatgc ctctgcctaa aggagacgca tcaaatttga aaagcttttc gataagaaaa
    10621 tccgctgccg ctattaccag ggcagcaata attgccgtag tgaagcctcc tctcattctg
    10681 aagccgggaa caaaataagc tgtaaccata agtacaattg cggcaactac gaatctgaca
    10741 atgaaaccaa ttactcctgt acctgtcgac cttctttgct gatcattctt aggtgtgtta
    10801 tttcccatgc aattcacctc ctgagtttaa tttaacctca gaaagataaa tatatacttc
    10861 tgtaatttga attctctatt ttttcttttt ctttactttt ttgccgttta cctgttcaat
    10921 ggtatttttt gtaccggaat aaagccctga tgcagaaagc ccatatacta tgcccttgaa
    10981 aacccctgct tttatacaca gaggttcaag aaaaaaaaca cctataataa ctccaataac
    11041 aacagctgca agagcacaaa acttcttggg cagcccaagc cctttggcaa gctgtgttaa
    11101 tgaaactata atcggcacca ctaatgtttc gctaatgttc atatatttac ccctcccttc
    11161 aatacataat atgatttgta ccaataaatg cgacagagca aaacagatgt ctttagggca
    11221 ttaaaaagga gca
//
LOCUS       DAOOAB010000067        11230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_7374_length_11230_cov_16.318837, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000067 DAOOAB010000000
VERSION     DAOOAB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_11230_cov_16.318837"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..757)
                     /locus_tag="PKU88_11320"
     CDS             complement(197..757)
                     /locus_tag="PKU88_11320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPX69898.1"
                     /translation="MDELSRVIAGCTGCRLHLSRTNTVPGEGNPNSRIMFVGEAPGAD
                     EDRLGRPFVGKAGQLLDKALNALDFYRDKNFYICNICKCRPENNRTPSDDEAEACLPY
                     LRRQVAIVKPQIIVCLGATPAKHIIDKQARITSIRGQWIERKGYHITATFHPAALFRD
                     ESKKRLFWEDLKKIKKKYDKIISSDL"
     gene            complement(936..3269)
                     /locus_tag="PKU88_11325"
     CDS             complement(936..3269)
                     /locus_tag="PKU88_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HPX69899.1"
                     /translation="MRLKAVYAKPYYRLDEWEIIEDSFDPDRNLHSETIFSIGNGFIG
                     IRGTFEEGLEDDSKPGAEGTFLNGVYEEGIIRYGEMAHGFPEKSQTMINAANGKKIIL
                     QLDDEIFNMQAGNILYYRRFINMREGILKRNLIWQSTMGKQIRLETERLVSFQRRNLA
                     VVRYKVTPLNFDGTIKIISEIDGDTKDALGEKDPRIGSSLEAGSFYTIDIVADSDTAA
                     LFQRTKRTGITVACVAKGAVRTECSYTSDKYICGDRVGFEYTVAGQKGKAIEYTKFIA
                     YEASIESCDSLMSHTLKVLESGMGKGYNELAREQREFLSDYWYRSDIEIKGDVALQQA
                     IRFNLFHLLQSAGRDGKTNICAKGLTGEGYEGHYFWDTEMYIIPAFLYSSPDISRKLL
                     EYRYGTLDKARERAREMSHKKGALFPWRTINGEECSGYFPAGTAQYHINAAIAHAIKK
                     YIEATQDDGFLLSYGAEILFETARLWADLGYFNAGKDGKFCINCVTGPDEYTAIVNNN
                     FYTNIMAKDNLEYAYNTAVWMREYHRPEYDRLAAEIGLGPEETELWKLAAGNMYLPYS
                     EKHGIHMQDDTFLDKKVWDFENIPRENYPLLLHYHPLVIYRHQVCKQADVVLALFLLG
                     HKFSLEQKRRDYEYYEKVTTHDSSLSACIFSIVANDIGYHEEAYDYFIKTARMDLDDY
                     QGNTHHGVHTANMAGSWMCIVNGFAGMRVYDDILSFKPYIHPDWESYSFKIAYRGRLI
                     KLTIRSGETSYELLGGNELEIAHRNKKYRLKDRLTLK"
     gene            complement(3304..4134)
                     /locus_tag="PKU88_11330"
     CDS             complement(3304..4134)
                     /locus_tag="PKU88_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPX69900.1"
                     /translation="MDKKSNKFKAMLFIFILLYLIVIIFPFIWVFLTSFKPTQEIFGE
                     GAFRLISESPTMENYANVLENGILQSMWNSFVVAGITSIYVVMVATLAAYAITRFKFR
                     GKNLLLGIILAVSMFPQMIIVGPIYNLFMNLNLTNSYFVVLPYSTITLPVAVFILVTH
                     FNKIPIALEEAATIDGATSMQILSKIVFRLAAPGIFTTAIIVFIAAWNEFLLTVTLNT
                     NKAYHTVPVAISFLRTQFTILWGDVTAATVIVTIPTLIIVLLFQKQIVSGLTSGAVKE
                     "
     gene            complement(4146..5357)
                     /locus_tag="PKU88_11335"
     CDS             complement(4146..5357)
                     /locus_tag="PKU88_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPX69901.1"
                     /translation="MKRMGFKEALYILPAIIIIAIFSLWPILKVINYSVFDYQLNDQQ
                     KSKLSLDEKFNVDLFNENADYVIMFVDMNRFSFPEDTQGKVDEVIYNVNEFKKILSDK
                     SGFIELSGELKEKVRANYNKLIEDTDFFINNYDFPDRENLKLVMEDTKKCFVQSNFIG
                     LAGYSNALKDERMHDALWHTVVFTIISVALELVLGLGLAIIMNKAMKGQGIVRAVSLI
                     PWAIPTTVAALMWSYLYDGSNGIVANILQNLHIISDSRNLLLSGGGAMASTILADVWK
                     TTPYMALLILAGFQTIPGTVYESAKVDGSGVFNTFFKITLPLLKPSILVALLMRTLDA
                     FRVFDLIYVLTGGGPGGSTETMSIYAYKTMFQQTNFGYGSVIVLLMVVCVAIIATFYI
                     KILGANAMERD"
     gene            complement(5444..6640)
                     /locus_tag="PKU88_11340"
     CDS             complement(5444..6640)
                     /locus_tag="PKU88_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPX69902.1"
                     /translation="MKKRVSLLLAIVMVFMAFCGFGMSVNADTRTVVKFAAQDDSTPA
                     TQILIDEFNKSQSKYKVEWVKMTNDSGQMHDQLMTSLSSGSSEYDVISMDVVWAGEFA
                     AAGFISPIDMNMYKDGLNKALYNAGSMAAGSYMGKQYTLPYFPDLGLLYFRSDIVSAT
                     DAAKLVTGNYTYDDLYNMAKKYKGRRGTTDGFVFQAKQYEGLTCNVTEFTGGLIDIKA
                     GLETMKKFVTSDFVPKDILNYTEAETHNSFINGKSVFARNWPYQYGMLADAPVKTYQV
                     GIAPLPKGSTVGGWLLGINKNSKNAEGAWEFIKYTSGWTGQMIMSRKGAYMPGYNYLL
                     TDERVENPLLSYEGLQNAMKATISRPISASYSKVSDTIQVNVHKYLSGGQDLNTTVTN
                     INNALR"
     gene            6763..7983
                     /locus_tag="PKU88_11345"
     CDS             6763..7983
                     /locus_tag="PKU88_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX69903.1"
                     /translation="MGDLKSIKGKLRTFHHATLMPINLLKGSKSVYCMPESINYISSI
                     IKDAYTIEDMPSLSPSGYSDNIKSYYFKNDYDECFIILHIKENYQLVAGPVLNEKIHE
                     GIINNIIRLNKLPIKIKSKLINYFENLLIVNTNRCYYCGKLLEYLFGTAVTDDLVGDT
                     SRERIFISDDYFTEMIKNREKLFHHPPYFLEQEMLSKIKSGNLNDTLAVLKEINSLKR
                     TRLSKDVLRSVKNSLICSVTLFTRAAIEGGVTPEAAFTLSDSIILAIEEINNMPELMD
                     YEYTAVKQYVQIVQDLSRIKYSNIIQQAISYIYKNLTNKLTLHKISDAIYVHPNYLSS
                     LFKRELGMSLSDYIMKTRVDESKYYIRYTNTKISDIAAFYQFCNQSYYTQVFRKFSGC
                     TPNEYRIKYNQQNE"
     gene            8067..8708
                     /gene="pgmB"
                     /locus_tag="PKU88_11350"
     CDS             8067..8708
                     /gene="pgmB"
                     /locus_tag="PKU88_11350"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HPX69904.1"
                     /translation="MSTIKGCIFDLDGVIVNTARYHYLAWKKLANELDFDFSEKDNES
                     LKGVSRMESLEILLKVGGLSFDDETKLALAARKNQWYVDYINSMEKDEVLPGVKGFLE
                     RARAAGLKIALGSASKNAPTILNRLKLADYFDVVIDGTKVSRAKPDPEIFLLAAQELG
                     LPPADCVVFEDAKAGVEAAKKAGMKCIGVGICSELADADAVIPGFENIDLSIL"
     gene            complement(8709..9356)
                     /locus_tag="PKU88_11355"
     CDS             complement(8709..9356)
                     /locus_tag="PKU88_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPX69905.1"
                     /translation="MLLQELREKVIEIALKAQKERLISMTMGNFSARDKETGYICITP
                     SGMEYDKLTAEDIVVMDAEGNILDGDRKPSIEHPLHCAVYRRRKDVFGVSHTHSVFAA
                     AWAACNENIPVVVAELAALVGGPVECAPYKPMGSSELAETAAVHLKDRHAILLANHGV
                     LAVGHDIEAAFANSVVVEEGAKIAYYARQIGVMKTIPEDECRSLRKSTLEKYGQK"
     gene            complement(9403..10551)
                     /locus_tag="PKU88_11360"
     CDS             complement(9403..10551)
                     /locus_tag="PKU88_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPX69906.1"
                     /translation="MNTVSIVKCDDYEFNNVKRAVYEALDEIDNIKLKIIKGSRILIK
                     TNLLMKKNPEDAVTTHPAVVEAIVRYLQDIGCKVIIGDSPGGPFTEWNLKSVYKAAGM
                     LGVAERTGCELNFDTSVLEISNPDTKRLKSMQIIKIAKEVDFVVSAAKLKTHGMMTYT
                     GAVKNLFGVIPGLVKADYHLRLNNTDNFADHLVDICETVRPVFSIIDGIDGMEGDGPS
                     AGEKRHAGLIMASESPYALDTAASSIVGIEPMTVPTIRAASLRNLFSGDLKDIDIKGV
                     RLDEIRIEPFKLPKSHNANLIGGRVPKAFERMVLNTLRPKPVFNHEICISCGDCKRSC
                     PPKIIDMSSGKPVPDLSRCIRCFCCHELCPKKAVDIRRHWIYDKVLKK"
     gene            complement(10560..11165)
                     /locus_tag="PKU88_11365"
     CDS             complement(10560..11165)
                     /locus_tag="PKU88_11365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPX69907.1"
                     /translation="MKKQELNNMNQLYRGRLEEHFGGEVSLVYGGGNIDSKIILVGEA
                     PGRTEVEQGKPFVGQAGKNLEEFINILGISREDLYITNVVKFRPVRYNQDTGRESNRT
                     PNKDEIRISTDFIEQELSIIKPELVVSLGNIALKGILKDDKATIGILHGSPIDVKFAG
                     TEFVLFPLYHPASIIYNRALKDVYLQDLHKLRNFMKKIKLI"
BASE COUNT         3087 a         2647 c         2042 g         3454 t
ORIGIN      
        1 tattctgagt ttcacttttt tcctgtcttt ttctgcttcg ccgattccga agcagagtta
       61 tattttacca tgttatatga taaatgtcaa taggcaattc ctcctatgct tatcagataa
      121 cataccttaa ttcgtccgtt caatgaagct ttttcttcaa tcttattatc tcacgctttc
      181 ctaattgtat atccctttat aaatcggaag atataatttt atcatacttt ttctttatct
      241 tttttaagtc ctcccaaaaa agcctttttt tgctttcatc tctgaataaa gctgcgggat
      301 gaaaggttgc tgttatatgg tatccctttc tttcaatcca ttgtcctctg attgatgtga
      361 ttcttgcttg tttatcaata atgtgttttg ctggagtagc tcccagacac acaattattt
      421 gaggcttcac tatcgcaacc tgcctgcgaa gataaggaag gcatgcctcg gcctcgtcat
      481 ctgaaggggt gcggttgttt tccggcctgc acttgcagat attacatata taaaaatttt
      541 tatctctata aaaatccaaa gcgttcagag ctttatccaa aagctgtcct gcttttccca
      601 caaagggtct tccaagtcta tcctcatccg ctcccggagc ttcgcctaca aacataattc
      661 ttgagtttgg attcccttca cccggcacag tatttgtcct actcaggtgc agccggcatc
      721 ctgtacatcc tgcaatcacc cttgagagtt catccatcgt attcatagtc atactcctct
      781 taatatgtat attcttatgg taattataat ataccatata tctcgtgcca aacattttac
      841 attttttgtt gataaatctt tttcacgtca tatattattt tttgataaag tacaactgct
      901 tttgagcata aaaatagact atttattcaa atagcctatt tcagcgttag tctatccttt
      961 agcctgtatt ttttattcct gtgagcaatc tccagttcat tacccccaag cagctcataa
     1021 gaggtctcgc cgcttcttat tgtaagcttt atcagtcttc ccctgtaggc aatcttgaag
     1081 ctgtacgact cccagtccgg atgaatatac ggtttgaagc tcaatatatc atcatatacc
     1141 ctcattccgg caaacccgtt cactatgcac atccagctgc ctgccatgtt ggccgtatgg
     1201 actccatgat gggtgttgcc ctggtaatca tccaaatcca ttcttgccgt ttttatgaaa
     1261 tagtcatagg cctcttcatg atagcctatg tcgttggcca ctatgctgaa tatgcatgct
     1321 gacagggaag aatcatgagt agtgaccttt tcgtagtatt cataatccct ccttttctgc
     1381 tccaagctga acttatgccc aagcaggaaa agcgccagta ccacatccgc ctgtttgcac
     1441 acctgatgcc tgtatatgac cagaggatga taatgtaata gtaaaggata gttctccctt
     1501 ggaatatttt caaaatccca taccttttta tccagaaaag tatcatcctg catatgtatt
     1561 ccatgcttct cactataggg cagatacata ttgccggccg caagcttcca cagctctgtt
     1621 tcttccggtc caagccctat ctctgccgca agcctgtcat actccggcct gtggtattcc
     1681 ctcatccata cagctgtatt ataagcatat tcaagattat cctttgccat aatattcgtg
     1741 taaaaattgt tgttgacaat tgcagtatat tcatcaggcc ctgtaacgca gtttatgcag
     1801 aacttgccgt ccttaccggc gttaaaataa cccaaatctg cccacaatct ggctgtttca
     1861 aagagtattt cagccccata gctaagcagg aacccgtcat cctgcgttgc ttcaatgtac
     1921 ttctttattg catgggcaat tgcagcgtta atatggtact gcgcagtccc tgcaggaaaa
     1981 tatcctgagc actcctcccc gtttattgtt ctccatggaa atagcgctcc cttcttgtga
     2041 gacatttccc ttgccctttc ccttgccttg tccagagtgc catatctgta ttcaagcagc
     2101 ttcctgctga tatcaggact gctgtacaaa aaggcgggta tgatatacat ctctgtatcc
     2161 cagaaatagt gtccttcata gccttctccc gtaaggccct tggcacagat atttgtcttt
     2221 ccgtctctgc ctgccgattg gagaagatgg aaaaggttga atcttattgc ctgctgcaag
     2281 gctacgtcgc cttttatttc aatatcagac ctataccagt aatccgacag aaactcacgc
     2341 tgctctcttg ccaattcatt atagcccttc cccattccac tttccagcac cttaagtgta
     2401 tggctcatca ggctgtcaca gctttctatg gaagcttcat atgctataaa cttggtatat
     2461 tcaatagctt ttcccttttg accggctaca gtgtattcaa atccaaccct gtctccgcat
     2521 atgtatttat cagatgtata tgagcactca gttcttacgg caccttttgc aacgcatgca
     2581 acggttatcc ctgtcctctt tgtcctttgg aaaagggccg ctgtatctga atccgccaca
     2641 atatcaatag tataaaaaga ccctgcctca aggctcgaac ctatcctcgg atccttttca
     2701 ccaagagcgt ctttcgtatc tccgtcaatc tccgagataa tttttatcgt accgtcaaaa
     2761 ttaagaggag ttactttata ccttacaact gccaggttcc ttctttgaaa gcttaccaat
     2821 ctctctgtct ccagcctgat ttgcttcccc atagttgact gccatatgag attgcgcttt
     2881 aatattcctt ccctcatatt gatgaaacgc ctataataaa ggatatttcc agcctgcatg
     2941 ttaaatattt catcgtccag ctggagtatg attttcttgc cattagcggc attaatcata
     3001 gtctggcttt tctcgggaaa accgtgtgcc atttcaccat atcttatgat tccctcttca
     3061 tatacgccgt tcaaaaaggt tccttccgcc cccggctttg aatcatcctc cagcccctct
     3121 tcaaaagtcc cccttattcc tatgaatcca ttcccaatgg aaaaaatagt ttcactatgt
     3181 aaattcctgt cagggtcaaa actgtcttca atgatctccc attcatcaag cctgtagtat
     3241 ggctttgcgt atacagcctt taatctcatg ttgtatgccc ctttctgatt ttgcagcgtt
     3301 cctctattcc ttgactgctc cggaggtcag gccggatact atctgcttct ggaataacag
     3361 tacaataatt aatgtgggta tagtaacaat aacggtagcc gcagtgacat caccccatag
     3421 tatggtgaac tgcgttctta agaaggaaat tgccaccggg acagtatgat aggctttatt
     3481 tgtatttaat gttacggtaa gcaggaattc attccatgcc gcaataaaca caattattgc
     3541 agtagtgaat atcccgggag ccgccagcct gaacactatc tttgataata tctgcatgga
     3601 tgtagcccca tcaatagtag cagcttcctc caatgcaata ggaatcttgt tgaaatgcgt
     3661 aaccagaata aacaccgcga cgggcaaagt tatggtggag tacggcagta caacaaaata
     3721 actgtttgtc agattcaaat tcatgaataa gttataaatt ggccctacta ttatcatctg
     3781 cggaaacatt gaaacagcaa gaataattcc aagaagcagg ttcttccccc tgaacttgaa
     3841 ccttgtaatt gcatatgcag ccagggtcgc taccataacc acataaattg aagttattcc
     3901 ggcaaccaca aaactgttcc acatggactg cagtatcccg ttttccagaa cattggcata
     3961 gttctccata gtcgggcttt ctgatattag cctgaaagca ccttcaccga atatctcctg
     4021 cgtaggcttg aaagaggtca ggaaaaccca gataaacgga aaaattatga caatcaaata
     4081 caacaatatg aatataaaca gcatagcctt gaatttattt gatttcttat ccattacgtc
     4141 accccctaat ccctttccat ggcatttgca ccgagtatct taatatagaa agtcgcaatt
     4201 attgcaacgc aaacaaccat aagcaggact ataactgaac cgtaaccaaa attggtctgc
     4261 tggaacatgg tcttgtatgc atatatggac atggtctcgg ttgaacctcc ggggccgcct
     4321 ccggtaagca catagataag gtcaaatacc ctgaaagcat ccagggttct cataagaagg
     4381 gccacaagta tagaaggctt cagcagggga agtgttattt taaagaaggt attgaatacc
     4441 cctgatccgt caacctttgc actttcatac accgttcccg gtatagtctg aaagcccgca
     4501 agtatgagca gagccatata gggtgtggtc ttccatacgt ccgcaagtat tgtagatgcc
     4561 atggctcctc cgccggacag cagcaaattc ctcgaatcgc ttataatatg aaggttttgc
     4621 agaatattcg ccactatacc gttgcttccg tcatacagat agctccacat aagggcggcg
     4681 acggtggtcg gtattgccca gggtatcagg gaaacggcac gtacaatacc ctgccctttc
     4741 atggctttgt tcatgattat tgccagtccg agcccaagta caagctcaag ggcgacagat
     4801 attattgtga atactacggt atgccaaagt gcatcgtgca ttctctcatc cttcagtgca
     4861 ttgctgtatc cggcaagccc tatgaaattt gactgtacaa agcacttttt ggtatcctcc
     4921 attaccagct tgaggttttc cctatccggg aaatcataat tgttgataaa aaaatccgta
     4981 tcctctataa gcttattgta attagccctg actttttctt taagttctcc gctaagctca
     5041 ataaatcctg acttatcaga caatattttt ttaaattcat tgacgttata tatcacttca
     5101 tctacttttc cctgagtatc ttccggaaaa ctgaatctgt tcatatcaac aaacattatc
     5161 acataatccg cattttcgtt aaaaaggtct acattaaact tttcatccag agaaagcttg
     5221 gatttctgct gatcgttcaa ctggtaatca aaaacggagt aattgattac cttaagtatg
     5281 ggccaaagtg agaagattgc tataataatg atagccggta atatatagag tgcttcctta
     5341 aaccccatcc ttttcatgtg accaccccca aagaaggaat ttcaaaagaa ttgggcccga
     5401 gtataaccgg agcccaattc ctctaagttc tttaaaccga atattacctc agggcattat
     5461 ttatatttgt tacagtagtg ttcagatcct gtcccccgga cagatactta tgaacattta
     5521 cctgtattgt atccgaaacc ttggaatagc tggctgaaat cggccttgat attgttgcct
     5581 tcatcgcatt ctgaagccct tcatatgaaa gaagcggatt ttcaactctt tcgtcagtaa
     5641 gcagatagtt atatccgggc atatatgcgc cctttctgga cataatcatt tgtcctgtcc
     5701 agcctgatgt gtatttaatg aattcccatg caccctcagc attttttgag tttttgttta
     5761 tgccaagcag ccatccgcct accgtgcttc ctttaggaag aggagctatt ccaacctggt
     5821 aagtctttac aggtgcgtct gccagcatac cgtactggta gggccagttc ctggcaaata
     5881 cagacttccc gtttataaat gaattgtggg tttctgcttc agtgtaattc aatatgtcct
     5941 ttggaacaaa atctgaggta acaaactttt tcattgtctc aaggccggct tttatatcaa
     6001 tgagcccgcc tgtaaactca gttacattgc aggtaagccc ttcatactgc tttgcctgga
     6061 agacaaaacc gtctgtagtt ccgcgtcttc ctttatattt cttagccata ttgtacaggt
     6121 catcgtacgt atagttccct gtcacaagct ttgctgcatc agttgcgctt actatgtccg
     6181 agcggaaata aagcagtccc agatctggga agtatgggag tgtgtactgc tttcccatat
     6241 aacttccggc tgccattgag cctgcattgt acaaagcctt gtttaaaccg tccttataca
     6301 tattcatatc aatggggctt ataaatccgg cggctgcaaa ttcacccgcc cagacaacgt
     6361 ccattgatat aacgtcatac tcactggagc ctgaggataa ggaagtcatc aactgatcat
     6421 gcatctgtcc cgaatcattt gtcatcttta cccattccac tttgtatttg ctttgggatt
     6481 tgttgaactc atcaataagt atctgagtag caggggttga gtcatcctgc gccgcgaatt
     6541 tgacaactgt cctggtatcg gcattaacgc tcatgccaaa gccgcagaaa gccatgaaaa
     6601 ccattacgat agccaataat aaagacactc ttttcttcat ttattaccct cctcgtataa
     6661 tttactaatt tcattctaaa ggaagccggg cattaattat accaatatat taatatttat
     6721 gttaaaatat taatattaac aatcgtgtaa aggagggtaa tcatggggga tcttaagagt
     6781 atcaaaggga agctccgaac ctttcaccat gcaactctga tgcccataaa cctgcttaag
     6841 ggcagtaaga gcgtatattg tatgcctgag agcatcaatt atataagcag cataataaaa
     6901 gatgcatata caattgaaga tatgcccagt ctttctccct ccggttattc tgataatatc
     6961 aaatcctatt actttaagaa tgattatgac gaatgcttta ttatcctgca tataaaagaa
     7021 aattaccaat tagttgcagg ccctgttctc aacgaaaaaa ttcatgaagg tataattaat
     7081 aatataataa gacttaacaa attaccaata aaaattaaga gcaagcttat aaattatttt
     7141 gaaaacttgc ttattgtaaa cacaaacagg tgctactact gcggaaagct tttggaatac
     7201 ctgttcggca ctgctgtaac tgatgacttg gttggagata cctcccggga acgtattttc
     7261 atctctgatg actacttcac cgaaatgatc aagaatagag agaagctttt ccatcaccct
     7321 ccatatttcc tggaacagga gatgctttca aaaataaagt ccggcaatct caacgatact
     7381 ctggcagtac taaaggagat aaactccctt aaaagaacaa ggttgtcaaa ggatgtcctg
     7441 agatcggtga aaaattctct catttgctcg gttactttgt tcaccagagc tgccattgaa
     7501 ggaggagtaa cccctgaagc cgcctttacc cttagcgatt ctataatact tgccattgaa
     7561 gaaataaaca atatgcctga gctgatggat tatgagtata ctgcggtaaa gcaatatgtg
     7621 cagatcgttc aggacttatc caggatcaaa tattcaaata taatacaaca agccatttcc
     7681 tatatttata agaatcttac caataagctt acgcttcaca agatatcaga tgccatatat
     7741 gtccacccca attacttatc cagcttattc aaaagggaac taggaatgag cctttctgat
     7801 tacattatga agacaagggt tgatgaatca aaatactata taagatatac aaataccaag
     7861 atatctgata tagctgcgtt ttatcagttc tgcaaccaaa gctactatac acaggttttc
     7921 agaaaatttt caggctgcac acccaatgag tatagaataa agtacaatca acagaatgaa
     7981 taattgtaaa tattcacaat tgcaacctga aggtaacttt tatataatct tactaattat
     8041 tctaatatat tacttcagga ggctgcatga gtacaataaa aggctgcata tttgatttgg
     8101 acggggtaat tgtcaatacc gcaaggtatc attatcttgc atggaaaaag cttgccaatg
     8161 agctggactt tgacttctcg gagaaagata acgaaagtct caagggcgta agcaggatgg
     8221 aatcccttga aattctcctc aaggttggag gtctgtcctt tgatgatgaa accaagcttg
     8281 ccctggctgc caggaaaaat cagtggtatg tcgattatat aaactccatg gaaaaggatg
     8341 aggtactccc cggggtaaag ggatttttag aaagggcaag agctgccggt ctgaaaattg
     8401 ctttgggctc tgcaagcaaa aacgcgccga ctatattaaa caggctgaag cttgcagatt
     8461 atttcgatgt cgtcattgac ggaacaaagg tatcaagggc aaaacccgat ccggaaatat
     8521 ttctgcttgc agcacaagag cttgggttac ctccggcaga ctgtgtggtt ttcgaagatg
     8581 ccaaagcagg ggtggaggcc gcaaaaaagg cgggcatgaa gtgtataggg gtaggtatat
     8641 gttctgagct tgctgatgct gatgcggtaa ttcccggctt tgaaaacata gacttaagca
     8701 tactgtaact atttttgacc atatttctcc aaggtgcttt tccttaagga tctgcattca
     8761 tcttccggaa tagtcttcat tacacctatc tgtcttgcat agtaggctat ctttgctccc
     8821 tcttcaacta caactgagtt tgcaaaagcc gcttctatat catgtcccac tgcaagaacg
     8881 ccgtggtttg ccaaaagaat ggcatgtctg tccttaaggt ggacagcggc tgtttcggca
     8941 agctcagaag aacccatggg cttatacggc gcacactcta ccggtcctcc tacaagtgct
     9001 gccaattcag caacaactac cggtatattc tcattgcaag ctgcccaggc tgcagcaaaa
     9061 accgaatgag tatgggatac cccgaataca tccttccgcc tcctgtacac agcacagtgc
     9121 aaaggatgtt ctattgaagg cttcctgtct ccatccagta tattgccctc cgcatccatt
     9181 accactatat cttcagccgt taatttgtca tactccatac cgcttggtgt aatgcagata
     9241 tatcctgtct ccttgtccct cgcactgaaa ttacccattg tcatggaaat caatctttcc
     9301 ttctgcgctt tcaaagcaat ttctattacc ttctcccgaa gctcctgcag cagcattgta
     9361 taaacctccc atcaatacac agtgaatatt ctttcactgc ttttacttct tcagcacttt
     9421 atcataaatc caatgtctcc ttatatccac agccttcttt gggcaaagct cgtgacagca
     9481 gaaacatctt atgcacctgc ttaaatccgg cacaggcttg ccgctgctca tgtctataat
     9541 tttcggagga cagcttctct tacagtctcc gcaggatatg catatttcat gattaaatac
     9601 cggtttgggc ctgagagtgt ttaagaccat tctttcaaaa gcctttggca cccttcctcc
     9661 tattaaattg gcattatgcg acttcggaag cttaaagggt tcaattctta tttcgtcaag
     9721 ccgcactcct tttatatcaa tatccttcaa atctccgctg aacaagttcc gcaatgaagc
     9781 cgccctgata gtaggcactg tcataggctc tatccctact atgcttgaag ccgcagtatc
     9841 aagagcatag gggctttctg aggccataat tagccctgca tgcctttttt caccggcgga
     9901 aggcccgtcc ccttccatcc cgtcaattcc gtcaatgatt gaaaaaaccg gccttacagt
     9961 ttcacaaata tccaccagat ggtcggcaaa attatcagta ttgttgagcc gcaaatggta
    10021 atccgctttt acaagcccgg gtataacacc aaagagattc tttaccgctc cggtgtatgt
    10081 catcatcccg tgagtcttca gcttagcggc tgacaccacg aaatcaactt ctttggctat
    10141 ttttataatc tgcatacttt tcagcctttt ggtatcggga tttgatatct ccagcacgga
    10201 tgtatcaaaa ttcagctcac agcctgtcct ctccgccacc ccaagcatac cggcggcttt
    10261 gtagacagat tttagattcc attcggtaaa aggccctccg gggctgtcgc cgattattac
    10321 cttgcagccg atatcctgaa ggtatctcac tatcgcctca actaccgccg gatgtgtcgt
    10381 aaccgcatcc tctggatttt ttttcattaa taggtttgtt tttataagga tcctggagcc
    10441 ttttattatt ttgagcttta tattatcaat ctcgtccagg gcttcataaa cggctctttt
    10501 tacattatta aattcatagt catcacattt gacaatgctt acagtattca tagctgctcc
    10561 tatatcagtt ttattttttt cataaaattc ctgagcttgt gcagatcctg caaatacaca
    10621 tcctttagcg ccctgttata tataatgctt gccggatgat ataaaggaaa cagcacaaat
    10681 tccgtgccgg caaacttaac atcgatgggt gaaccgtgca atatgcctat tgttgctttg
    10741 tcatccttca atataccctt aagtgcaata tttcccaagc tgactacaag ttcaggcttt
    10801 attattgaca gctcctgttc tataaagtct gtagatatcc ttatttcatc cttattcggc
    10861 gttctgttgg attcccttcc tgtatcctga ttgtatctta caggtctgaa cttaacaaca
    10921 ttagtaatat ataaatcctc ccgggaaata cccagtatgt ttatgaactc ctccaggttt
    10981 tttcccgcct gtcccacaaa aggtttccct tgttccacct cagttcttcc gggtgcctca
    11041 cccaccagaa taatttttga atcaatattt ccaccaccgt ataccagtga tacttctcct
    11101 ccgaaatgtt cctccagcct gccccggtaa agttgattca tattattaag ttcctgcttt
    11161 ttcaaatcaa tcccctgctt tcccggactt taaattaatt gtgaaaaaat ttataaaaac
    11221 accggcaagt
//
LOCUS       DAOOAB010000068        11121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_7463_length_11121_cov_16.512742, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000068 DAOOAB010000000
VERSION     DAOOAB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7463_length_11121_cov_16.512742"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..3286)
                     /gene="carB"
                     /locus_tag="PKU88_11370"
     CDS             complement(62..3286)
                     /gene="carB"
                     /locus_tag="PKU88_11370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPX69908.1"
                     /translation="MPKRTDINKILIIGSGPIIIGQACEFDYSGTQACKALRNLGYKI
                     VLVNSNPATIMTDPGIADSTYIEPLNTKSLTEIIDKERPDAILPNLGGQTALNLCLEL
                     YKSGILDKYYVKVIGVQINAIERGEDRIAFKETMNRLGIDMPCSKAAYSVSEAEKIAG
                     ELGYPVVIRPAYTMGGTGGGLVYNAEELRTIASRGIAASMVGQVLVEESVLGWEELEL
                     EAVRDSKNQMITVCFIENIDAIGVHTGDSFCAAPMLTISPELQQRLQEYAYSVVESIE
                     VIGGTNVQFAHDPRTGRVVIIEINPRTSRSSALASKATGFPIAYVSAMLAAGLTLDEI
                     PYWRDGSLDKYTPSGDYVVIKFARWAFEKFPGAQDKLGTQMRAVGEVMSIGKSYKEAF
                     QKAIRSLEINRYGLGFAGNFNNNTLYELLSMLTEPTSQRQFIMYEALRKGADVEQLYK
                     LTHIKPYFIEQMKELVLLEEEILKCKGSELQDELLIKAKKDGFSDRYLAQLLDVSEAL
                     VRRKRTGLGLTEGWEAVPVSGVENASYYFSSYNISDKTSVTDKPKVMILGGGPNRIGQ
                     GIEFDYCCVHAAFELRDLGYETIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYEK
                     EKPLGVIVQFGGQTPLNIAGDLEKAGVRILGTSQETIDLAEDRDRFRRIMDKLDIPMP
                     ESGMAVNAEGAIAIANQIGYPLMVRPSYVLGGRGMQIVHDEEMLCTYMAAAVGVTPER
                     PILVDKFLGHAIETEADAISNGADTFVPTVMEHIEYAGVHSGDSACIIPPVGISEEHI
                     STIVDYTGRIAKEMNVIGLMNMQYAIADNKVYVLEANPRASRTVPLVSKVCNIQMARI
                     ATRIIMADCLGGRYSLDGLASKKIPHYGVKEAVLPFNMFPEVDPVLGPEMRSTGEVLG
                     ISDSFELAYYKAQEATQELLPSLGTVLISVNDADKPDVLEAAREFSRIGFKIVATEGT
                     CKFLQEKGISCEKVNKVYEGRPNIADNMENDEINLIINTPQTRLSEFDDSYIRKIAIK
                     RKVPYITTMTAALASAKGIAAYIKNGFHGKKIKSLQEYHRDIAE"
     gene            complement(3587..4393)
                     /locus_tag="PKU88_11375"
     CDS             complement(3587..4393)
                     /locus_tag="PKU88_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPX69909.1"
                     /translation="MNFLSGFFKGLLIGIGGVAPGVSGGAIAVIFGLYERITDAIADV
                     FKDFKKNAVFFFPIALGGGVGVLGFSKIMQYLFQYYEVQVKCLFIGLMLGTLPTVFRQ
                     ANKKGFKGIYIIPCIIAFGITIITVVLENAGIDIIPDAAPGMLQLVLYGAIIGVGTIV
                     PGISASFVLMYFGAYQIVLDGLANINLSILVPVGTGFVFSIILFAKFISLLFKKAYGF
                     TYYAVIGFVIGSIISIFPGISFSSEYLISILLFIMGYIITFLISKYSKDT"
     gene            complement(4452..4973)
                     /locus_tag="PKU88_11380"
     CDS             complement(4452..4973)
                     /locus_tag="PKU88_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPX69910.1"
                     /translation="MDFLELAKMRYSVRNYLPRKVEEEKLLKILEAARVAPTGSNRQP
                     HRLIVVQEAESLEKLRKSANIYGAPLTIIVCADHNISWKRSYDGKDVADIDAAIVTDH
                     MMLEATQLELGTLWICHFNPKVLKAEFSIPENLEPMNILAIGYSADAALSPERHQELR
                     LPLEDFVLYESYK"
     gene            complement(5072..8191)
                     /locus_tag="PKU88_11385"
     CDS             complement(5072..8191)
                     /locus_tag="PKU88_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPX69911.1"
                     /translation="MKIHEVSIRRPVAILMCVLMVLVLGGVSFSRLPIDLMPEMDFPI
                     SIVLTSYSGVGPQEIENIITRNIESAVATVNNIKTIQSQSSEGQSIVIAEFNSGTDMN
                     FATLQMREKIDMVKGMLPDGVEAPMIIKMDPGMIPAAVLGVSGGENEAELTRFVEDKI
                     KAKIESIDGVASVAVTGGTTKEIKVNLDSQKLSGYGLSFSQISGALRSENLNQPGGTV
                     EYGDKSLLVRSTGEFQSLDQIRNIPITLPTGAVVYLRDMAEVKEGYKSVDTYNRMNGE
                     SSIGLTVQKQTDSNTVKVVNLIKKEINNILKEYPDVKVEMVFDQGEYVEQSIANVAKN
                     AVVGAILAIIILFVFLKNIRTTMIIGTSIPISIIATFVMIYFSGITLNLVSLGGLALG
                     VGMLVDNAIVVLENIYRYRNEGHSRVEAAMLGTQEVGSAVLASTLTTVVVFLPIVFTG
                     GIAAEIFKQMALAVTFSLIASLLVALTLIPMLSSKFLKMVKYHEASRSERLNKVFDKW
                     EDTLSAVDNFYRSILSWALAHRRKTVITVLAIFAGSLLLIPFVGTEFFPNMDMGQFTV
                     GIELPQGSLLKNTNEVAEQVEGMLNSLPEIQKVFTTVGSSGSGMDLSGGASNTASLNI
                     TLKPLSERKRSTAEVVEEMRNKVRLIPGADIEINEVSTSAMGVSGGTAVSVKICGPDL
                     DKLSEIAADAEELIKKVEGVRQTNTSISEGRPEAQIYVNRDKASYYGLGTAQVSAAIR
                     TAVEGSVATTYKVDGDEIDVRVGFPEETGEKYSQLIDTKILSPLGVEVPLTEIADIKM
                     EKGPISITREGQERYVTVSSDVFGRSTGDVNKDISERLDEMLLPPGYSIKYGGEAEQI
                     ADAFSSLTLALLLAIVLVYMVMAAQFESLLHPFTIMFSLPLAFTGSVLGLVLTGRALS
                     VPAFIGVIMLAGIVVNNAIVLVDYINTLRGRGMDRLEAILKAGPTRLRPILMTTLTTI
                     LGLIPLALGIGEGAEAQAPMATVVIGGLTTSTVLTLVIIPVIYTLFDDVSVKLRRHKA
                     GSIRKTEAV"
     gene            complement(8205..9461)
                     /locus_tag="PKU88_11390"
     CDS             complement(8205..9461)
                     /locus_tag="PKU88_11390"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPX69912.1"
                     /translation="MKRKYTKLIIGIVVVTLLGSLIFTYVKNKAGSSANINADTVVAV
                     SVEAAAKTSVSATVNLNGKLKPIQEINIIPKLPGKVNNIYFDIGQSVKAGDVLFTLED
                     NDIRSQLKQAQAGLDMANSGLAKVTGGGAELQELQLKAALTSAEISYNDAKLAFERTK
                     QLYESGAVSKATLEQSESQFKLMEQQYNSAKSNYEITTTKTNPENIASAKAQVSQAAA
                     ALELAKSQLDNTVIKSPINGVIAARVIEIGELAGSTGIAMSVIDLSSVLIDINATEGM
                     INKIQVGDKAEVTIKSVGDSKFTGEIINISPTIDTRTQSYPVRIKIDNSSGTLKGGMF
                     AEVKTVLDKVSDVIAVPISSVIEEGDKRFVYVLNGDIAEKREVSTGLFDDELIVIVKG
                     LAENDAVIVKGQELIIDGSKVIVTEN"
     gene            complement(9487..9933)
                     /locus_tag="PKU88_11395"
     CDS             complement(9487..9933)
                     /locus_tag="PKU88_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPX69913.1"
                     /translation="MDIKDKCDRMMKIAMDIHKKLMAVILKDIEPSLSKSHVYLLAYI
                     QRKGECTVTDIANHLEITLSAVTSLVDKLCTAGLVTRLRSVEDRRVVYMELTPEGEKV
                     LEKIEANKNRLSEKIFSNIDTEEINNFFATIEKITQNIIDSYSSEN"
     gene            10151..>11121
                     /locus_tag="PKU88_11400"
     CDS             10151..>11121
                     /locus_tag="PKU88_11400"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HPX69914.1"
                     /translation="MLYIRNENTNPFFNLAMEEYLFNLNDNNDYVLLWQNEPTIVVGK
                     YQNTAEEINSEFVKEKGIHVVRRITGGGAVYHDLGNLNFTFINKGTGKKEFDFRKFTM
                     PIVKALERFGVKAELSGRNDITIDQKKFSGNAQYVKQGKVLHHGTLLFNSKMEELIKA
                     LKVTEDKFQSKGIKSVRSRVTNIADYLSENITVSEFKELLLKYMFDEDTELIEGQLKD
                     ADMNEINLLMKSKYMNWDWNYGASPEFNVKQAKRFEGGKVEVLINIKNGVIQSIKFYG
                     DFFGSESPEEIEALLRGKRYNEDEIRGAIAPLNINEYFKGISPEELL"
BASE COUNT         3084 a         2600 c         1984 g         3453 t
ORIGIN      
        1 agcgatttcg tgaaacagcc gtgtctcccg tgttacccgt tacctatggc gcattcttct
       61 tctattctgc aatgtctctg tgatactcct gcaaagattt aatcttcttt ccatgaaacc
      121 cattttttat atatgccgca attccctttg cgcttgccag agccgcggtc attgtggtta
      181 tatacggcac cttgcgcttt atagcaatct tgcggatata agaatcatcg aattcgctca
      241 gtctggtttg aggtgtattg attatcaggt ttatttcatc gttctccatg ttgtcagcta
      301 tgtttggccg gccttcgtat accttattga ccttttcaca gctgattcct ttttcttgaa
      361 ggaatttgca ggttccttcc gtagcaacga ttttaaagcc tatcctcgaa aattctctgg
      421 cagcctccag aacgtcaggc ttatctgcat catttacact aataagcact gttcctaaag
      481 aaggaagtaa ttcctgcgtc gcttcctgag ccttatagta tgccaattcg aatgaatccg
      541 agatgcctag tacttccccg gtagaacgca tttccgggcc caataccgga tcgacctcag
      601 gaaacatatt gaagggcaga actgcttcct taactccgta gtgaggtatt tttttgctgg
      661 caaggccgtc caaggagtat cttcctccca agcagtcagc catgattatt cttgtggcta
      721 tacgtgccat ttgtatattg cagactttgg aaacaagggg aacagttctt gaggcccttg
      781 gattggcttc caaaacataa accttgttat ccgctatggc atactgcatg ttcataaggc
      841 cgataacatt catttccttg gcaatccttc cggtatagtc aacaatagta cttatgtgct
      901 cttcagaaat acctacggga ggaataatac atgcggaatc tccggaatga acgccggcat
      961 actcaatatg ttccattact gtcggcacaa atgtatctgc tccgttggaa attgcatctg
     1021 cctccgtttc aatggcatgc ccgaggaatt tatctaccag aatcggcctt tcgggagtga
     1081 ctccaaccgc cgcagccata taggtgcata gcatttcctc gtcatgcact atttgcattc
     1141 cccgcccgcc caatacatat gacggacgaa ccattaaagg ataacctatt tggtttgcta
     1201 tggctatggc tccttcggca tttaccgcca tacccgactc cggcatggga atgtcaagct
     1261 tatccataat tctgcggaat ctgtccctgt cctcagccag gtctatggtt tcctgggagg
     1321 ttccaagaat ccttacaccg gccttttcca gatcccccgc aatattgaga ggagtctggc
     1381 ctccgaattg aactatcact cccagaggct tttccttctc ataaatgctt aatacatcct
     1441 ccactgtcag gggctcaaag taaagcttat cagatgtatc ataatcagtt gaaacagttt
     1501 cagggttgca gttgaccatt atcgtttcat agcctaaatc tctaagttca aaggctgcgt
     1561 gaacgcagca gtaatcaaac tcaattccct ggccaattct gttggggcct cctccaagaa
     1621 tcataacttt tggtttgtcc gttacggaag tcttatcgga tatgttatag gaagagaaat
     1681 aataggacgc attctcaaca ccgcttacag gcactgcctc ccaaccctct gtcaggccta
     1741 atccggttct tttccttcgg actaatgcct ctgacacatc aagaagctgg gccagatacc
     1801 ggtcggaaaa accatccttt ttggccttga taagcagttc atcctgcagt tcgcttcctt
     1861 tgcacttaag tatttcctct tcaagcagaa caagctcctt catctgctct atgaaatagg
     1921 gttttatatg cgtcagctta taaagctgtt caacatcagc acccttgcgc aatgcttcat
     1981 acataatgaa ctgccgctgg ctggtcggct cagtcagcat ggaaagaagt tcataaagag
     2041 tattattatt gaaattcccg gcaaagccca agccgtaacg attgatctcc agggaacgga
     2101 ttgccttctg gaaagcctcc ttatagcttt tcccgatgct cataacctcc ccaaccgcac
     2161 gcatttgggt tcccagttta tcttgggctc cggggaactt ttcaaaagcc cagcgggcaa
     2221 actttattac aacataatct cctgatgggg tatatttatc caggctcccg tccctccaat
     2281 aaggaatctc atcaagggta aggccggctg caagcatggc ggatacgtat gcaataggga
     2341 agcctgttgc ctttgaagcc agtgccgagg aacgcgaagt gcgggggttt atttctataa
     2401 ttacaaccct tccggttctt gggtcatgag cgaactggac attggttccg ccgattacct
     2461 caatagattc caccactgaa taagcgtact cttgcaagcg ctgctgaagc tcaggactga
     2521 ttgtgagcat cggagcggca cagaaggaat ctcccgtatg cactccgata gcatcaatat
     2581 tttcgataaa gcagactgta atcatctgat tcttgctgtc cctcactgct tcaagctcca
     2641 gttcctccca gcccagaacc gattcttcaa ccagaacctg ccccaccatg ctggcagcaa
     2701 ttccgcggct ggcaatagtc ctcagttctt cagcattata taccaggccg ccgcctgtgc
     2761 cgcccattgt ataagcaggc ctgataacca caggataccc aagctcccct gcaattttct
     2821 cagcttcgga aacgctgtag gctgccttgc tgcatggcat atcaatccca agcctgttca
     2881 ttgtctcttt gaaagcaatg cggtcctctc cgcgttcaat ggcatttatt tgaacaccta
     2941 taacctttac gtagtattta tccaggatgc cgctcttata aagctccaga caaagattga
     3001 gagctgtctg gcctcccaga ttcggcagta ttgcatcagg cctttcttta tcaattattt
     3061 ccgtgaggct tttggtgtta aggggttcaa tgtaggtcga atctgcaatt cccgggtcgg
     3121 tcataatagt agcggggttg gaattgacca gtacgatttt atagcccaag tttctcaggg
     3181 ctttgcaggc ttgtgtcccg gaatagtcaa attcacaggc ttgcccgata atgataggac
     3241 cggatccgat aatcagaatt ttatttatgt cagttctttt cggcatataa atcctcctat
     3301 tactgtagtt aataagtcat ggatgtaaat aaggatagca aagagattca catatgaaca
     3361 ctccttgata tcccatgatc cggaaaaggt ctgcacatat atttcctcct ttctggcctc
     3421 gcaggaccaa tttaaagttt gaaattactg tttgaaaata taaacctata tgcatatata
     3481 taatataata tgtatattta tgcttgtcaa tatttttttg cctgtaagta aaaaaagaca
     3541 tggagacttt tcttatgccc ccacgccctt tgttactctg tccgttttat gtgtcctttg
     3601 aatatttgct tattaaaaat gttataatat agcccatgat gaaaagcaaa atgctgatta
     3661 aatattcaga actgaatgaa atgcccggaa atatcgaaat tatagatccg attacaaatc
     3721 caatcacggc ataatatgta aaaccgtatg cttttttgaa caaaaggctt atgaacttgg
     3781 caaatagaat tatgctgaat acaaatcctg tccctacagg tacaagaatt gacagattaa
     3841 tatttgcaag tccgtcaaga actatctgat atgctccaaa atacatcagc acaaaggaag
     3901 cactgattcc cggcactatc gtccctactc ctataattgc accgtataaa acaagctgaa
     3961 gcattcccgg tgctgcatca ggaataatgt cgattccggc attttccagc actactgtta
     4021 ttattgtgat tccaaatgca attatacaag ggataatgta tatgccttta aagcctttct
     4081 tgttggcctg cctgaaaact gtaggaagcg ttcccagcat aagcccgatg aaaaggcatt
     4141 tgacctgaac ctcatagtat tgaaaaaggt attgcattat tttgctgaaa ccgagtaccc
     4201 ccacaccgcc gccaagtgca atagggaaaa agaatactgc gtttttctta aaatccttga
     4261 agacatccgc aatggcatcg gttatcctct catataatcc aaatattacg gcaattgctc
     4321 ctccgcttac cccgggagca acgccgccta tccctatcag caatccctta aaaaagcccg
     4381 ataaaaaatt catttctcct cactcacctt tatgcttttg tttttaccat aggtatttcg
     4441 tataaaatta tttatttata cgattcgtaa agcacaaagt cttcaagggg cagtctgagc
     4501 tcttgatgcc tttccggaga tagtgctgca tctgcggaat aacctattgc aagaatattc
     4561 atcggttcaa ggttttcggg gatactaaac tctgctttga gtacctttgg attaaagtga
     4621 catatccata gagttccaag ctccagttgt gtagcttcaa gcatcatatg gtcagttaca
     4681 atagccgcat caatatcagc tacatctttt ccatcatagc tccttttcca tgaaatatta
     4741 tggtctgcgc acacaatgat agtcagtgga gctccatata tattagctga ttttctcagt
     4801 ttttctaggc tttcggcttc ttgaaccaca atcaatctgt ggggctgcct gttggatcct
     4861 gtaggcgcta ccctggctgc ttcaagtatc ttcaatagct tttcctcttc aaccttcctt
     4921 ggtaaatagt tcctcactga gtatctcatt ttagccaatt ccaaaaaatc catatcatac
     4981 accttccctt acaagcatct tttataatat tctacactaa ttcttcccaa agaaaaagca
     5041 ctgcattcaa aaagcggtgc tttttacaaa tctagactgc ttctgttttt cttatactcc
     5101 ctgctttatg cctgcgaagt tttacagaca catcatcaaa caatgtatag attaccggga
     5161 ttattacaag agtaagaaca gttgaagtcg taagtcctcc gataacaact gttgccatag
     5221 gtgcctgggc ttctgcccct tctcctatac ccagcgcaag ggggataagc cctaatattg
     5281 ttgttaaagt cgtcatcaat atcggtctca atctggtagg accggcttta agtattgcct
     5341 ccagcctatc catacccctg ccccttaatg tatttatata gtccacaagt actatagcat
     5401 tgtttacaac tatacctgca agcattatta ctccgatgaa agccggaacg cttaaagccc
     5461 tccccgttaa aacgagtccc aatacagacc ctgtaaaagc gagaggcaga gagaacatga
     5521 ttgtaaaggg atgcagcaag gattcaaatt gtgcggccat aaccatatat accagaacaa
     5581 ttgcgagcag caatgcaaga gtcaagcttg agaatgcatc tgcaatctgc tccgcctcac
     5641 cgccgtactt tattgaatac cccggtggaa gaagcatttc atccagcctt tcactaatat
     5701 ccttgttaac atctccggta ctccttccaa acacatctga tgaaacagtt acatatcttt
     5761 cctgaccctc ccgggttata gaaatcggtc ccttttccat tttaatatca gcaatctctg
     5821 taagaggtac ctcaactccc aagggtgata gtatctttgt atcgattagc tgcgaatact
     5881 tttcccccgt ttcttcggga aaacctactc ttacatctat ttcgtcccca tcaaccttgt
     5941 aggttgtcgc aacacttccc tcaacggctg ttcttattgc agccgacacc tgtgcagtac
     6001 ccaaaccata ataggatgcc ttatctctgt ttacatatat ttgagcttcc ggtcttccct
     6061 ctgaaatgct tgtatttgtc tgtcttacgc cctctacttt ttttattagc tcttcagcat
     6121 cagctgctat ctccgaaagc ttatccaaat ccggtccgca tattttgaca gaaaccgcag
     6181 tacctccgga tactcccata gccgatgtgc tgacttcatt gatctctata tcagctccgg
     6241 gaattagtct taccttattt ctcatctcct caactacctc tgcggtactt ctttttctct
     6301 cacttaaagg ctttaaggta atattcaaac ttgctgtatt tgatgcccct ccggataaat
     6361 ccattccgct tcctgaagaa ccaaccgttg taaatacctt ttgaatctcc ggaaggctgt
     6421 ttagcattcc ttcaacctgc tctgcgactt cattagtatt cttaagtaaa ctcccttgtg
     6481 gaagctcaat ccctaccgta aactgaccca tatccatatt ggggaagaac tcggtaccca
     6541 caaaaggaat cagcagcaaa cttcctgcaa atattgccaa cactgtaata actgtttttc
     6601 ttctatgtgc taatgcccag cttaatatgc ttctgtaaaa attatcaact gcacttagag
     6661 tatcttccca cttgtcaaac actttattta atctttcgct tcttgaagct tcgtgatatt
     6721 ttaccatctt caaaaatttg gagctaagca taggaataag tgttaaggct acaagcaaag
     6781 aagcaataag tgaaaaggtt actgccagtg ccatctgttt gaatatttct gctgctatac
     6841 ctccggtaaa tactataggc agaaatacaa ccactgttgt cagggttgat gccaatacag
     6901 cggaacctac ctcctgtgtc cccagcattg cggcctctac cctgctgtga ccttcattcc
     6961 tatacctgta tatattctcc aggacaacta tggcattgtc aaccagcatt ccaaccccca
     7021 gggcaagtcc tccaagggat acaagattta gtgttatacc ggaaaaatat atcattacaa
     7081 aggttgctat tatggaaatc ggtatggacg tcccgattat catagttgtt cttatattct
     7141 tcaagaacac aaaaagtata ataatagcca gtatagctcc tacgacagca ttttttgcaa
     7201 cattcgctat tgactgctcc acatattctc cctgatcaaa aaccatttca acctttacat
     7261 ccggatattc cttcaatata ttatttattt ctttctttat aagattaact acttttaccg
     7321 tattggaatc tgtctgcttt tgaaccgtaa gtcctatgct gctctcacca ttcattctgt
     7381 tatacgtatc aacactttta tatccttctt ttacttcagc catatcccgc agatatacaa
     7441 ctgctcctgt gggaagcgta atggggatat tcctgatttg gtcaagactt tgaaattctc
     7501 ctgtgcttct gactaaaaga ctcttatctc catattccac tgtgccccca ggctggttaa
     7561 gattctctga tctgagagct ccgctaatct ggctgaagga caatccatat cctgataatt
     7621 tttgagaatc caaatttact ttaatctctt ttgtcgtacc gccggtaact gcaacagatg
     7681 caactccatc aatgctttcg atttttgctt ttatcttgtc ctcaacgaac cttgtcagct
     7741 ctgcttcatt ctcgcccccg gagactccaa gtacagctgc gggaatcatg ccgggatcca
     7801 tctttataat cataggggct tctactccat ctggcagcat tccctttacc atatcaatct
     7861 tttctctcat ctgcaatgtt gcgaagttca tatcggttcc cgagttgaat tctgcaataa
     7921 caatagattg cccttccgag gattgtgatt gtattgtttt tatattattt accgtagcaa
     7981 ctgcactttc tatattcctt gttataatat tctcaatttc ctgaggtccg actcctgaat
     8041 agcttgttaa aactatagat ataggaaaat ccatttccgg cattaaatct ataggaagcc
     8101 tggaaaagga tacccccccc agaaccaaaa ccatcagcac acacattaaa attgctacag
     8161 gtcttcttat tgacacttca tgtattttca tccaaacacc atccctaatt ttcggtaact
     8221 ataaccttgg agccatcaat aatcagttcc tggcccttta cgattacagc atcattttcc
     8281 gcaagtcctt ttactatcac aataagctca tcatcaaaca gacctgttga aacttccctt
     8341 ttttctgcga tatcgccatt aagaacatat acaaatctct tatcgccctc ttcaattacg
     8401 gaggaaatcg gaaccgctat aacatcagac actttatcca atactgtttt tacttctgca
     8461 aacatacctc ccttaagagt tccggagcta ttatctatct taattcttac aggatagctt
     8521 tgagttcttg tatctattgt cggagatata ttaattattt ctcctgtaaa tttactatct
     8581 cctactgact tgattgttac ctctgcctta tccccaacct gtattttatt tatcatacct
     8641 tcagttgcat ttatatcaat gagtactgag gataaatcta ttacactcat agcaataccg
     8701 gtgctgcctg caagctcacc aatttcaata actcttgcag caattactcc gtttataggt
     8761 gatttgatta ccgtattgtc caattgggat ttagcaagct caagagctgc tgctgcctga
     8821 cttacctgtg ctttggccga tgcaatgttt tcaggatttg tttttgttgt cgtaatctca
     8881 tagtttgatt ttgcagaatt atactgctgt tccataagct taaactgtga ctccgactgt
     8941 tccagtgttg ccttagatac ggcaccggat tcatataact gttttgttct ctcaaaagct
     9001 agctttgcat cattatagct tatttctgcc gaagtcagtg ccgctttaag ctgcagctcc
     9061 tgcagctctg ctcctcctcc tgttacttta gccaatcctg aatttgccat gtcaagccct
     9121 gcctgagctt gcttcagctg cgaccttata tcattatcct ccaaagtaaa caagacatct
     9181 ccggctttaa cactttgccc aatgtcaaag tatatattat ttaccttccc cggtaatttg
     9241 ggaataatgt ttatttcctg aatcggctta agttttccgt tcaaattgac agttgcactt
     9301 acacttgttt tagctgccgc ttcaacgctt actgcaacga ctgtatcagc atttatattt
     9361 gcagagctgc cagctttgtt tttcacatat gtaaaaatta gcgaaccaag taaagtaacg
     9421 actactatac ctattatcag ttttgtatat ttccttttca ctatttaaac ccccatacta
     9481 aatctattaa ttttcactac tataactgtc tataatattt tgagtaattt tttcaattgt
     9541 ggcaaaaaaa ttatttattt cttcagtatc aatatttgaa aaaattttct cggacagtct
     9601 attcttgtta gcctcaattt tttccagaac cttttctccc tccggcgtaa gctccatata
     9661 aacaaccctt ctgtcctcaa cgcttctaag tcttgtgacc aatcccgcag tgcacagctt
     9721 atcaacaaga cttgttacag cacttaatgt aatttccaga tgatttgcta tgtccgttac
     9781 agtacattcc cccttgcgct gtatatatgc caaaagatag acatgagact tgcttaatga
     9841 tggttcaata tccttaagta tcacagccat cagcttttta tgaatatcca tggctatctt
     9901 catcatcctg tcacatttat cttttatatc caaattaata aacctcctta attattaagc
     9961 cacttaacta taaaacaatt ttattataat gcagtagaat accaattgca acatactgct
    10021 gtaaaaattt ttgagtgaat tctattaatg ttaccgctcc taaggttatt cgatttagtt
    10081 ctcaatagca tggatttatt atgctataat aataatatta ttcaataatg acattaccgg
    10141 gaggtacaac atgctgtaca taagaaatga aaacacaaat ccctttttta atctggcaat
    10201 ggaagaatat ctgtttaacc tgaatgataa taatgattat gtacttttat ggcagaacga
    10261 gccgacaata gtagtgggga aatatcagaa taccgcagaa gagattaaca gtgaatttgt
    10321 aaaagaaaaa ggtattcatg tagtaaggag aataaccgga ggaggtgcgg tttaccatga
    10381 cctgggtaac cttaatttta cctttataaa caaaggaacg ggtaaaaagg agtttgactt
    10441 caggaaattt accatgccca ttgttaaagc cctggaacgt tttggtgtaa aggctgagct
    10501 ttcaggccga aatgatatta ccatagatca aaaaaagttt tccggaaatg cacaatatgt
    10561 aaaacaggga aaggttctgc accacggaac cttacttttc aattccaaaa tggaggaact
    10621 gataaaggca ttgaaggtaa ccgaggataa gttccaatcc aagggaataa agtccgtaag
    10681 gtcaagagtc acaaatattg cggattatct gtccgagaat attacagttt ctgagtttaa
    10741 agagctgctt ttaaaatata tgtttgatga ggacacggaa ttaattgaag gacagcttaa
    10801 agatgcagac atgaatgaaa taaacttgtt gatgaaaagc aaatatatga actgggattg
    10861 gaattacgga gcttcacctg agtttaatgt caaacaggca aaaaggtttg aaggcggcaa
    10921 agttgaagta ttgataaaca taaaaaacgg tgttatccaa agcataaagt tttatggaga
    10981 tttcttcggt agtgaaagcc ctgaagaaat tgaggctttg ctaaggggaa aacgatacaa
    11041 tgaagatgaa ataaggggtg ctatcgcccc cttgaatatt aatgagtatt tcaaaggcat
    11101 cagccctgaa gaattattaa g
//
LOCUS       DAOOAB010000069        10936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_7602_length_10936_cov_15.902858, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000069 DAOOAB010000000
VERSION     DAOOAB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_10936_cov_15.902858"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1300
                     /gene="leuC"
                     /locus_tag="PKU88_11405"
     CDS             35..1300
                     /gene="leuC"
                     /locus_tag="PKU88_11405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPX69915.1"
                     /translation="MGMTMTQKILAAHAGIEEVHPGQMIKARLDLVLGNDITAPVAIE
                     EFNRIGANRVFDKSKVAIVPDHFTPNKDIKSAENCKLVREFSRKMEIENYFEIGQMGI
                     EHALIPEKGLAVPGDVIIGADSHTCTYGALGAFSTGIGSTDMAVGMATGEAWFKVPGA
                     IKFILKGKPGKWVSGKDIILHIIGMIGVDGALYQSMEYSGDGVGHLSMDDRFTIANMA
                     IEAGAKNGIFEADGKTLEYVSEHSERIPKIFKAGADAEYNRVIEIDLGAIKPTVAFPH
                     LPENARSIDEVGDIRIDQVVIGSCTNGRLEDLRTAAKVLKGSKVSSSVRTIIIPATQK
                     IYLEAMKEGLLEIFIEAGAAVSTPTCGPCLGGHMGILAKGERAVATTNRNFVGRMGHP
                     ESEIYLASPAVAAASAITGKISSPEEVLG"
     gene            1297..1791
                     /gene="leuD"
                     /locus_tag="PKU88_11410"
     CDS             1297..1791
                     /gene="leuD"
                     /locus_tag="PKU88_11410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPX69916.1"
                     /translation="MIKGRAFKYGDNVDTDVIIPARYLNITDKAELASHCMEDIDCDF
                     VKAVKPGDIIAAGKNFGCGSSREHAPLVIKLSGIKCIIAESFARIFFRNAINIGLPIL
                     ECAAAAKDMEKGDLVEADIKTGRITNITKGRTYQAESFPEFMREIMKAEGLINYVRKR
                     GKGL"
     gene            1788..2858
                     /gene="leuB"
                     /locus_tag="PKU88_11415"
     CDS             1788..2858
                     /gene="leuB"
                     /locus_tag="PKU88_11415"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPX69917.1"
                     /translation="MKIALIKGDGIGPEVTEQALKVLYAVSERFKCKFEYREALLGGR
                     AIDETGVPLPDETVDICAGSDAVFLGAVGGPKWDGLPGNLRPEAGLLGIRKALKLFAN
                     LRPVMLFPQLRTASPLKAEVLSQGFDIMIVRELTGGAYFGEKKRVETKDGIKAWDMME
                     YTSREIERIAGIAFGIARKRRKRITIVDKANVLESSRLWREVTGSIMKDYGDVETAYM
                     YVDNAAMQLIRNPGQFDVILTENLFGDILSDEASMLAGSLGMLPSASLGASKACLFEP
                     IHGSAPDIAGKGIANPLAAILSCGMLLKYGFGMEKESDAVLEAVNNALDKGYRTADIM
                     QPGMTLVGTEKMGSIVAENILI"
     gene            2874..4553
                     /gene="ilvB"
                     /locus_tag="PKU88_11420"
     CDS             2874..4553
                     /gene="ilvB"
                     /locus_tag="PKU88_11420"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPX69918.1"
                     /translation="MRLNCAEALLECLREQGVDTIFGYPGGTVINIYDALYERKDITH
                     ILTAHEQGAAHAADGYARATGKVGVVLATSGPGAANTVTGIATASRDSVPMVVITGQV
                     ARSLLGKDSFQELNITSITKTITKKNYLVMNPETLPKIVREAFEIARSGRPGPVLIDI
                     PKDVQQAVIEYEPAEADTGYDTSNLPEMSCETDLYLDKAVELINNSKRPLIYAGGGVK
                     ISDSCRELLEFAEKIKAPVACSLMGTGVFPGTHPYFTGLAGMHGSRCSNIAICNCDLL
                     IAVGARFSDRTICKADSFAPYANIIHIDIDPEELGKNVDTKIPLCGDVKKYLRLLLER
                     IDEKKEGIWNKQIQSWKEAYIVSYKSKGSLNPQYLMKKLYELTQGKSIITTEVGQNQM
                     WTAQYYPFADPRTFISSGGLGTMGYGLGAAIGAAVGKPDCKVINIAGDGSFRMNLSEL
                     STITKYKLPIVQVVLNNSSLGMVRQWQKIFHGERYSHTSLADEVSFTKLADAFGIKSA
                     KITENDEVEKAFKEAFDMNEPVVIECIIHPDDLVLPMVPAGEPINECIDDF"
     gene            4575..>4729
                     /locus_tag="PKU88_11425"
     CDS             4575..>4729
                     /locus_tag="PKU88_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HPX69919.1"
                     /translation="MDESYVFFNGKIVPEKEVSISIRSKVVNYGLACFEGIRAYWDEE
                     GQQLYGF"
     assembly_gap    4730..4829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4830..5479
                     /locus_tag="PKU88_11430"
     CDS             <4830..5479
                     /locus_tag="PKU88_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPX69920.1"
                     /translation="GFRTTVYIRPLAYKGGEGLNPTLLDDDNRLLIYCQPLGNFSGKQ
                     EMHVAFTSWQRLRDNMLPPRTKASAAYLNSALASLEVLRNGFDEALFLTDNGNVCEGP
                     GENIFMVKKGKLVTPPPSDNILEGITRETIITLAKEEFGMEVVERSIPRNELFSAQEV
                     FFSGTAMEVMPIVEVDRRTIGAGRPGDICTKLKSLFFEITVGKNPKYAGFCTPVY"
     gene            5603..7201
                     /locus_tag="PKU88_11435"
     CDS             5603..7201
                     /locus_tag="PKU88_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPX69921.1"
                     /translation="MPAAQQELQKEIRRRRTFAIISHPDAGKTTLTEKLLLYGGAIRL
                     AGSVKARKAQKYAVSDWMEIEKQRGISVTSSVMQFEYNGYCINILDTPGHQDFSEDTY
                     RTLMAADSAVMIIDSAKGVEEQTRKLFHVCKMRGIPIFTFINKMDRAGKDPFELAEEI
                     EKVLGIQSFPMNWPIRVGGSFLGIYDRKLSQIELFNGGDHGQSIVGSSKGNVDDPVFS
                     DLLGEDVHNKLIDDIMLLDVAGDEFSRRKVLKGELTPVFFGSAMTNFGVQVFLEEFLE
                     LTTPPLSRNADIGEIDAEKEEFSGFIFKIQANMNPTHRDRIAFLRICSGKFTKGMEVN
                     HVQGGKMIRLSQPQQFLAQDREIVEEAYAGDIIGVFDPGIFKIGDTLCEGGSFRFEGI
                     PVFAPEHFARVYTKNSLKRKQFMKGIEQISEEGAIQLFRQKDAITQEFIIGVVGILQL
                     EVLEYRLKNEYGVDISMEHLPYKYIRWVKNPEFNPETFRITTDTMIVQDQSLNPVLLF
                     QNEWSIRTVTERNNGVELVEILSR"
     gene            7361..10075
                     /locus_tag="PKU88_11440"
     CDS             7361..10075
                     /locus_tag="PKU88_11440"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HPX69922.1"
                     /translation="MNLNWYNKGIQAISEELSTDLQKGLSREEAAGRIEKYGYNELKE
                     KEKEPVWVKIGRQLKDFLVVILIIASIVSGMVGEISDSIVIIAIVIVNAVLGVVQEGK
                     AEKAMEALKKMSAPNARALRNGHIETVPAKNLVPGDVVFIEAGDSIPADMRLIESSNL
                     KVEEASLTGESVPSEKDASKAFDTEVSIGDRLNMAYMSTIVTYGRGKGIVVNTGTNTE
                     IGRIAEAIQTFEDETTPLQQKLNELGRWLGLACLIICVIVFAIGFMRGGDILELFLVS
                     VSLAVAAIPEGLPAVVTIVLALGMKRMVRRNAIVRKLLAVETLGCVTVICSDKTGTLT
                     QNEMTVVRAFTDGNIYKISGQGYNPTGEITFEDEKIKPEAEPGLKLLLSAGVLCNDST
                     LEQKDARENLWGIVGDPTEGALVVAGAKAGFDKSKMNEAHVRIEEIPFDSERKMMTTF
                     HNNFIQNKIVSFTKGAPDIILGRCAKMLQNGSVIDMTEALKEQIAKVNSEFAGDALRV
                     LAFTYREYDKLPESISPDSIENNLIFIGLMGMIDPARVEAFEAIKTCKEAGIKPVMIT
                     GDHKDTAVAIAAELGLMEEHSGVLTGNELDKLSDEELFNRVENTSVYARVSPEHKVRI
                     VEMLKKRGHIAAMTGDGVNDAMALKKADIGVSMGITGTDVAKGTADIILMDDNFATIV
                     SAVEEGRIIYGNIRKFVFFLLSCNIAEIFIIFLSILFNMPVPLVPIQLLWLNLVSDSF
                     PALALGTEAGEPGIMKKKPRDTREPILNKSLSTGILILSIAQTAAVLIAFRWALNNYD
                     KDLETARTIAFTTLVVVELVMAFTCRSERYPVIKLGIFSNRALVLAAITSLVLGLAVL
                     YIPFLQPIFKTVSLGIRDWVMILLLSIIPVLTGEIYKMIVGRAEKAAQ"
BASE COUNT         3245 a         2015 c         2926 g         2650 t
ORIGIN      
        1 ctgggggaat gggagaaaag ggaggcaaaa aaatatggga atgacaatga cgcaaaaaat
       61 tctggcagct catgcaggaa ttgaggaagt gcatccgggg cagatgataa aagccaggct
      121 tgacctggtg cttggcaatg atatcacagc accggttgca attgaagagt tcaacaggat
      181 aggggcaaac agggtgtttg acaaaagcaa ggttgcaatt gtacctgacc acttcactcc
      241 caacaaggat attaaatctg cggagaactg caaactggtc agggagtttt caagaaaaat
      301 ggaaattgag aattattttg aaatagggca aatggggata gagcatgcac taatcccgga
      361 aaagggcctg gcagttccgg gggatgtcat aataggagct gattcccaca cctgtaccta
      421 tggagccctg ggagcatttt caacaggaat aggaagtacc gatatggctg tgggtatggc
      481 gacgggagaa gcatggttca aggtgccggg agcaataaag ttcatattga aaggcaagcc
      541 ggggaaatgg gtcagcggaa aggatattat actccatatc ataggaatga taggggtgga
      601 cggagctttg taccaatcta tggagtacag cggggacgga gtcgggcacc tttccatgga
      661 tgaccggttc accattgcaa atatggcaat agaggccgga gcaaaaaacg gaatttttga
      721 agctgacggc aaaactctgg aatatgtttc ggagcattcg gaaagaatcc caaagatttt
      781 caaggccggc gcagatgcag aatacaacag ggtaatagaa attgacttgg gagcaatcaa
      841 accgacagtt gcatttccgc atcttccgga gaatgcgaga agtatagatg aggtgggaga
      901 tataaggatc gaccaggtag ttatagggtc atgcaccaac ggcaggcttg aggacctcag
      961 aacggctgca aaagtattga agggcagcaa agtaagttca agtgtaagga caataattat
     1021 tccggcaacc cagaagatat atctggaggc aatgaaggaa ggtttgcttg agatattcat
     1081 tgaagcagga gcggcagtaa gtaccccgac ctgcgggccc tgcctcggag gccacatggg
     1141 aatactggca aagggggaaa gggcagtggc aactaccaac aggaattttg taggtaggat
     1201 ggggcatccg gaaagtgaga tataccttgc aagtccggca gtagcagcgg cttccgccat
     1261 taccggcaaa atttcttctc ctgaggaggt gctcggatga taaaaggcag agcttttaaa
     1321 tacggagata atgtggatac ggatgttatt atcccggcaa gatatttgaa cataaccgat
     1381 aaggctgagc ttgcctctca ctgtatggag gacatagact gtgattttgt aaaggctgtg
     1441 aagccaggag acataattgc ggcaggcaaa aatttcggct gcggttcttc cagggaacac
     1501 gcgccgttgg taataaagct ttccggaatc aagtgcatca tagctgagtc ctttgcacgg
     1561 attttcttca gaaatgccat aaatataggg cttcccatac ttgaatgcgc tgctgcagca
     1621 aaggatatgg aaaagggtga cctggtggaa gcggacatta agacaggaag aataacaaat
     1681 atcaccaaag gccggaccta tcaggctgaa tccttccctg aatttatgag ggaaataatg
     1741 aaagccgaag gattgataaa ttatgtaagg aaacggggga agggtctatg aaaattgctt
     1801 tgataaaggg agacggaatc gggccggagg taactgaaca agcattaaag gtgctttatg
     1861 cagtatcgga aagatttaaa tgcaagtttg aataccggga agctctgttg gggggcaggg
     1921 caattgatga gacgggagtg ccgctgcccg atgagactgt tgatatttgc gcgggaagcg
     1981 atgcggtgtt tctgggcgcc gtaggggggc cgaaatggga cggccttccc gggaacctga
     2041 ggccggaagc tgggcttctg ggtatcagaa aagctctcaa attatttgcc aatctgaggc
     2101 cggtaatgct ttttccgcag cttagaaccg cttccccgct gaaagcggag gtactgtcac
     2161 aaggctttga tataatgatt gttcgtgagc ttacgggggg cgcatatttt ggcgaaaaga
     2221 aaagagtcga gacaaaggac ggaatcaagg catgggacat gatggaatac acatcccgtg
     2281 aaattgaaag gattgcagga atagcttttg gcattgcaag aaagagaaga aagagaatta
     2341 ctattgttga caaggcaaat gtcctcgaaa gctcaaggct gtggcgtgag gttacaggaa
     2401 gcattatgaa ggattatggg gatgtggaaa cagcatatat gtatgtggac aatgcggcaa
     2461 tgcagcttat caggaatccg gggcagttcg atgtgatact cacggagaac ctgttcgggg
     2521 acatattaag cgatgaggca tctatgcttg ccggttctct cgggatgctg ccttcagcca
     2581 gcctgggagc ctcaaaagcc tgtttgtttg aacctataca cggctcagca cctgatattg
     2641 cgggaaaggg cattgcaaat cccctggctg caatactcag ctgcggaatg ctgctgaaat
     2701 acggcttcgg aatggagaag gagtcagatg cagtccttga ggctgtaaat aatgctttgg
     2761 acaaaggata caggacagct gacataatgc agccgggaat gactttagtc gggacggaaa
     2821 agatgggcag tattgttgca gaaaacattc tgatttagtt tggaggtgat gaaatgagat
     2881 tgaattgtgc tgaggcactt ttggaatgcc tccgggaaca gggagtcgat acgatattcg
     2941 gatacccggg aggcacggtt ataaatattt atgatgctct ttacgaaagg aaggacataa
     3001 cccatatatt gactgcccat gagcagggtg cagctcatgc tgcggacgga tacgcaaggg
     3061 caacggggaa ggtaggagtt gtcctggcaa cttcaggccc gggggctgcc aacactgtaa
     3121 cgggaatagc aactgcatcc agagactcag ttccgatggt tgtaatcacg gggcaggtgg
     3181 caaggtcgct tcttggcaaa gattcctttc aggagcttaa tattacaagc attacaaaga
     3241 ccataacaaa aaagaactat ctggtaatga atccggaaac acttccgaaa attgtcaggg
     3301 aagcttttga gattgcaagg tccgggcgtc cgggaccggt actcattgat attcccaagg
     3361 atgtgcagca ggctgtaata gaatatgagc ctgcggaggc agataccggc tatgacacct
     3421 ccaatctgcc ggaaatgagc tgtgaaacgg acttatacct ggataaggca gttgaattga
     3481 taaacaacag taagaggccg ttgatttatg cgggaggcgg tgtcaagatt tcggattcct
     3541 gccgggaact gctggaattc gcggagaaga taaaggctcc tgtggcttgc tcattaatgg
     3601 gtacgggggt attcccggga actcatcctt attttacagg cctggccggc atgcatgggt
     3661 cacgatgctc aaatattgcc atatgcaact gcgatcttct catagctgtc ggggcacggt
     3721 tcagtgacag gacaatatgc aaggctgaca gctttgctcc ttatgccaat attattcata
     3781 tagatattga cccggaggaa ttggggaaaa acgtagatac aaaaattccc ctttgcggtg
     3841 atgttaagaa atatctgagg cttttactgg agagaattga cgaaaagaag gagggcatct
     3901 ggaataaaca gatacaaagc tggaaagagg cttatattgt ttcatataaa agcaaaggat
     3961 ccttgaatcc ccaatatttg atgaaaaagc tttatgagct tactcaaggc aagagtataa
     4021 taactaccga ggtggggcag aatcagatgt ggacggcaca gtattatccc tttgccgatc
     4081 ccaggacatt catatcatcg ggagggcttg gaacaatggg ctacggcctt ggtgcggcaa
     4141 taggcgcagc agtgggaaag cctgattgca aggtgattaa tatagcagga gacggaagct
     4201 tcaggatgaa tctttccgag ctttctacca ttacaaaata caagcttccg attgttcagg
     4261 tggttttgaa taacagttct ctggggatgg tgcgccagtg gcagaagatt ttccacggcg
     4321 aaaggtattc ccatacaagc cttgcggatg aagtaagctt tacaaagctt gccgatgcct
     4381 ttgggataaa gtcggcgaaa atcactgaaa atgatgaagt cgaaaaggct tttaaagaag
     4441 cttttgatat gaatgagcct gttgtaatag aatgcatcat acatccggat gacctggtgc
     4501 ttccgatggt tccggcggga gagcctataa atgagtgtat agatgatttt tagaaaaact
     4561 aaggagatgt aattatggac gaatcatatg tttttttcaa tggaaaaata gtaccggaaa
     4621 aagaagtgag tataagtata agaagcaaag tggtcaatta tgggcttgcc tgctttgagg
     4681 gaataagagc atattgggat gaagaaggac agcagctgta cggcttcagn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cggcttcagg acaacggtgt atataaggcc
     4861 tttggcatat aaaggcggtg aagggttgaa tcctacactg ttggatgatg acaacagact
     4921 gcttatttac tgccagcctc tggggaattt ttcgggcaag caggaaatgc atgttgcatt
     4981 tacttcatgg cagaggctca gggataatat gctgcctcca aggactaagg catcagcggc
     5041 atacctgaat tcggcactgg cgtccctgga agtgcttcga aacggatttg atgaggcatt
     5101 attcctcact gataacggaa atgtatgtga agggcccgga gaaaatattt tcatggtgaa
     5161 aaaaggaaaa ctggtaaccc cgccgccttc agacaatata cttgagggaa taacaagaga
     5221 aacgattatc actctcgcaa aggaagagtt tggaatggag gtagtagaga gaagcatccc
     5281 aaggaatgag cttttctcgg cacaggaggt gttcttcagc ggaactgcta tggaagtaat
     5341 gccgatagtt gaagtggaca ggaggactat aggtgcaggc aggccggggg atatatgtac
     5401 taagctgaag agcctgtttt ttgaaattac cgtaggtaaa aatcccaaat atgccggctt
     5461 ctgcactccg gtatattgat gctttaattg acactctatt gctgttcgtg taaaattaat
     5521 aagtcacgga atgtgatact tgggatttga tgaacaagtt aaaaatataa aagtatatac
     5581 tgaacaatag aggagtaata gaatgccggc tgcacaacag gaattacaaa aggaaatcag
     5641 aaggcgaaga acctttgcca taatatccca ccctgatgcg ggaaagacaa ctttgacaga
     5701 aaagcttttg ctgtacggag gcgccattcg ccttgcgggt tctgtaaaag ccaggaaagc
     5761 tcagaagtac gccgtatccg actggatgga gattgagaaa cagaggggaa tctctgttac
     5821 ttcaagtgta atgcagtttg aatataacgg ttattgcatt aatattcttg atacacccgg
     5881 gcatcaggat ttcagcgaag atacttacag aacacttatg gcggcagaca gcgcggtgat
     5941 gataattgac tcggcaaagg gtgttgagga acagaccagg aagctgttcc acgtatgcaa
     6001 aatgagggga ataccgattt ttacctttat taataaaatg gacagggcag ggaaggatcc
     6061 ctttgaactg gcggaagaaa tcgagaaggt gctgggaatt cagtcatttc ctatgaactg
     6121 gccaataaga gtcggaggaa gctttctggg tatttacgac agaaagctgt cccagataga
     6181 gctgtttaac ggcggtgacc atggacaaag catagtaggg tcctctaaag gcaatgttga
     6241 cgatcctgtc ttttctgatc ttcttggaga ggatgttcat aataagctta ttgatgatat
     6301 aatgctgctg gatgttgcgg gagacgagtt cagcagaaga aaagtcctga agggggagct
     6361 gactccggtg ttttttggca gtgccatgac caacttcgga gttcaggtgt ttcttgagga
     6421 atttcttgaa ttaacaacac cgcctctttc cagaaatgcg gacatcggtg agatagatgc
     6481 ggagaaggaa gagttctccg gattcatttt taaaatccag gcaaatatga atcctactca
     6541 tagagacagg atagcgtttc ttaggatttg ttccgggaaa ttcaccaaag gcatggaagt
     6601 caaccatgta caggggggca agatgataag gctgtctcag ccgcagcagt tcctggccca
     6661 ggatagggag attgttgaag aggcttatgc aggggatatc atcggagtat tcgatcccgg
     6721 aattttcaag ataggagaca ccttgtgcga gggaggcagc ttcaggttcg aaggaatacc
     6781 cgtatttgca ccggagcatt ttgcaagggt gtataccaaa aactccctga agcgcaagca
     6841 gtttatgaag ggtatagagc agatatcgga ggaaggcgcc atccagttat tccgccaaaa
     6901 agatgccata acccaggaat ttattattgg agttgtgggt atacttcagc tggaggtatt
     6961 ggaatacagg ctgaaaaatg aatacggcgt ggatataagc atggagcatt tgccctataa
     7021 gtatataaga tgggtaaaga atccggaatt taatccggaa acctttagga ttacaactga
     7081 tacaatgatt gtacaggatc aatccttgaa ccctgtgctg cttttccaga atgaatggtc
     7141 catcaggacg gtaacggaga gaaataacgg tgtagagctg gttgagatac ttagcagata
     7201 atatatttga ttttagaggc ctttggtgca aaaaattgtt tttgcgtcaa aggcattttt
     7261 attgtatagg aaagtatact ttcctataca atgggggatg caatgtaata attcaggctg
     7321 ataaacaaat aataaaatat tatcatatgg aggggattta atgaacctga actggtacaa
     7381 caagggtatt caggcaattt ccgaggagct ttcgacagat ttgcagaaag gcctttccag
     7441 agaggaagcc gccggcagga ttgaaaagta cgggtataat gaactgaagg aaaaggaaaa
     7501 agaaccggta tgggttaaaa tcggcagaca gcttaaggat ttccttgtgg taatacttat
     7561 tatcgccagt atagtctccg gaatggttgg agaaatatcc gactctattg taataattgc
     7621 aatagtaatt gtaaatgctg tcctgggggt tgtgcaggaa ggaaaagcgg agaaagccat
     7681 ggaagcgctt aaaaaaatgt cggctccaaa tgcaagggcg cttagaaacg gacatattga
     7741 gacagtcccc gcaaaaaacc ttgtacccgg ggatgtcgta tttattgagg cgggagacag
     7801 catacctgcc gatatgaggc ttatagaaag ctctaacctt aaagtggagg aagcctcact
     7861 tacgggggaa tcagtaccgt cggaaaagga tgcaagcaag gcctttgata ctgaggtttc
     7921 aataggcgac aggctcaaca tggcatacat gagcaccata gtaacctatg gcaggggcaa
     7981 aggaatagtg gtcaacacag gaacaaatac tgagatcggc agaatagccg aagcaattca
     8041 gacttttgaa gatgaaacaa cccccctgca gcaaaagctg aatgagttgg gtagatggct
     8101 ggggctggcc tgccttataa tatgtgtcat tgtttttgcc attgggttta tgagaggcgg
     8161 cgatatcctg gagttgttcc tggtatcagt aagccttgcg gttgctgcaa tacctgaagg
     8221 attaccggct gttgtcacaa ttgtacttgc gctgggaatg aagaggatgg tgcggagaaa
     8281 cgccatagtc agaaagctcc ttgcggttga gactcttgga tgtgtaacgg taatatgctc
     8341 ggacaagacc ggtactctta cacagaacga aatgaccgtg gtgagagctt ttacggatgg
     8401 aaatatctat aaaatatcgg gacagggata caatccgaca ggagagatta catttgaaga
     8461 cgaaaaaatc aaacctgagg cagaacccgg gttgaagctc ctgctttctg cgggagtatt
     8521 atgcaatgac tccacccttg agcagaaaga tgccagggaa aacctctggg ggatagtggg
     8581 cgatcctacc gaaggagcac ttgtcgttgc aggtgcaaag gcaggctttg ataagtcaaa
     8641 aatgaacgag gcccatgtca ggattgaaga gatacccttt gattccgaaa gaaaaatgat
     8701 gactacattc cataataatt tcattcaaaa caaaatagtg tcttttacaa agggtgctcc
     8761 cgatataata ctcggcaggt gcgctaaaat gctccaaaac ggcagcgtga ttgacatgac
     8821 cgaagctttg aaggagcaaa tagccaaagt taacagcgag tttgcgggag atgcccttag
     8881 agtacttgcc tttacctata gggaatacga taagctgcct gaatcaataa gcccggattc
     8941 gatagaaaat aacctgatat ttatcgggct tatgggaatg attgatcctg ccagggttga
     9001 ggcctttgag gcgataaaga catgtaaaga agccggaata aaaccggtta tgattacagg
     9061 ggatcataag gacactgctg tggctattgc cgcggagctt gggcttatgg aagagcattc
     9121 cggtgtactg acaggtaacg aactggataa attatccgat gaagagcttt tcaacagagt
     9181 cgaaaatacc agtgtatatg caagagtatc tcccgagcat aaggtgagaa tcgtggaaat
     9241 gcttaaaaag agggggcata ttgcggctat gaccggggac ggagttaatg atgccatggc
     9301 tttgaagaag gcggatatag gtgtttcaat ggggataacc ggcaccgatg ttgccaaggg
     9361 taccgcagat ataatactta tggatgacaa ttttgccaca atagtatcgg cagttgaaga
     9421 aggcagaatc atatacggca atataaggaa gtttgtcttt ttccttctgt cctgtaatat
     9481 tgccgagata tttattattt tcttaagcat attgtttaat atgcccgtgc ctctggttcc
     9541 tatacagctt ttatggctta accttgtatc agacagtttt ccggctcttg ccctgggcac
     9601 cgaagcggga gagcccggta taatgaagaa aaaaccgagg gatacaaggg agccgattct
     9661 gaataaaagc ctttcaacag ggatattgat actcagcata gcccagacag cggctgtgct
     9721 tatagccttc cggtgggctt tgaacaatta tgataaggat ttggaaactg cgcgaactat
     9781 agcttttaca actcttgtgg ttgttgaact tgttatggct tttacctgca ggtccgagcg
     9841 atacccggtc ataaagcttg ggattttcag caacagggca ctggtattgg ctgctataac
     9901 atctcttgtg cttggtttgg cagtgttgta tattcctttt ctgcagccca tattcaagac
     9961 tgtttcattg ggaatcaggg attgggttat gatactgctc ctcagtatta ttccggtact
    10021 gaccggcgaa atatacaaga tgattgtagg cagggcggag aaagcggcac agtagaagaa
    10081 gaatgcgtcg caacataagt ttgctatacc tcttcaagag acggtatagt atagcgatgc
    10141 acacggatta tgattttgac aggcctactg tgaagcaaaa caaattaata cgaatccgcc
    10201 tcacgccggt taatttgtcg cttttcttag ttatcgccat tgattggcgc cggccaatct
    10261 gcttcctctg ccttgagaat cagcaaattt cctcggcggg aggtcaatag attttcgagc
    10321 aagctgattt gttttgtggc aaggcgggcg ctcgcagtaa tgccgtacgc attacaagag
    10381 cggacaacac agtcacagga caaatcagct gctccaaaac ggatttgtat tagtttgttt
    10441 tgcttggcct ttggaaaagt tctaacggtg tcaaaaggtc gtccatgacc cgagccacct
    10501 aagccggcgt ccatgccggc ttcacgaact ttttaccaaa gccatatcag aggcctgttg
    10561 ccaaaatcat aaaagtatgc cctgctatac tatacagctc ttgcttggta tgtatattaa
    10621 ggagcactac cttgtatata taggaaatgc aaataccgac gtggggaaga tatcttcgag
    10681 gaaaccttcc acctgacctc ggaacgggaa tacctccagg caaaataacc agacggaccc
    10741 gcagagatgg gacccccgcc agtgattttg ttggctttgg aaggtgagga gaacatactt
    10801 cccctacagt cggagtactt cacaaactca ttatgtcgca ttattctact gatgttcaac
    10861 agccccttta taatatgtta aaaatatatt aatatagatg tagatgcaga tatagatata
    10921 gaagattaat gcgtgt
//
LOCUS       DAOOAB010000070         9997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_8428_length_9997_cov_15.113056, whole genome shotgun sequence.
ACCESSION   DAOOAB010000070 DAOOAB010000000
VERSION     DAOOAB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8428_length_9997_cov_15.113056"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1335)
                     /locus_tag="PKU88_11445"
     CDS             complement(271..1335)
                     /locus_tag="PKU88_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX69923.1"
                     /translation="MSLAVDIKKKLKGFSLDVSFRTEGEYLGILGASGSGKSLTLKCI
                     AGIETPDEGCIVLNGKVLYDSEKKINLKPQERNIGYLFQNYALFPHMTVEENIGMGLR
                     LPEKEKKQKVNEMIVSFHLQGLEKKYPNKISGGQQQRVALARCIVYKPDILMLDEPFS
                     ALDSHLREQLQDGVLELLKLYEGEVLMVTHSRGEVYSFCSRLVVLDNGRSILHGDTKD
                     IFRQPKLYGAARLTGCKNISKCEVLSPKNVYAADWDVILETEQKVPQQVKYVGIRAHD
                     LQITDAGQAKEKNVMECRINRIVEDVFEYNVFFENRKSNSENDNGEILYKADKQEWDN
                     IRDKDNLYLRFPESAILLLE"
     gene            complement(1416..2087)
                     /gene="modB"
                     /locus_tag="PKU88_11450"
     CDS             complement(1416..2087)
                     /gene="modB"
                     /locus_tag="PKU88_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HPX69924.1"
                     /translation="MQMSPIIISLKTASASIALTFLSGLYVARWIVRMKSDKAKMILD
                     GILTLPLVLPPTVAGFLLLIVFGVNRPVGKFLLQFLGTKIVFSWSATVIAAAVISFPL
                     MYRSARGAFEQVDQELIMAARTLGMSERRIFWEIIMPLALPGVAGGGILAFARGLGEF
                     GATAMIAGNIENKTRTLPLAIYSEVAAGNMQAASYYVIIVMLISFVVVVLTNYFSIRE
                     RKYFK"
     gene            complement(2099..2875)
                     /gene="modA"
                     /locus_tag="PKU88_11455"
     CDS             complement(2099..2875)
                     /gene="modA"
                     /locus_tag="PKU88_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPX69925.1"
                     /translation="MKRTVAIALFALCIVLASTGCSNNAAAKESTITVAAAASLKNCI
                     DGEVIPMFKEKNPGINVLATYDSSGKLQTQIEEGASIDVFMSAAMKQMDALNEKGLIE
                     ENSIVQLLENKIVLIVPENSTKGITAFEDILKADKIAVGDPASVPAGQYAKEALTNLN
                     IWEEISKKASLGTNVTEVLNWVAEGSADAGIVYSTDAASNKKVKVAAEAPEGTVSKVI
                     YPAGIVKASKEMDASKKFVEFLKTDEAKKVFESYGFTAIK"
     gene            3043..>3359
                     /locus_tag="PKU88_11460"
     CDS             3043..>3359
                     /locus_tag="PKU88_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX69926.1"
                     /translation="MNEDVLYTPEEIALKLRITKGTVYEMIKRGELEAHRIGRYIRIS
                     QTQFELYLLKAKGYENIYEAVISRESGGTFANIGSVKIYVDTALEGQVKVSVRPENII
                     LSMG"
     assembly_gap    3360..3459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3460..3994)
                     /locus_tag="PKU88_11465"
     CDS             complement(<3460..3994)
                     /locus_tag="PKU88_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPX69927.1"
                     /translation="MHNCLENKDYNLICVIVNFGLGSDVLKLSKQHGVTGGTIFLGKG
                     TVKSAILELLELSESRKEIVLMAAEAAIEDTVLEELNRKFKFSKPNHGIAFTTSIKSI
                     LGIARCGCKIDNVSGGAENPMYNLIMAIVERGKAEAAVEAARDAGARGGTIIHARGSG
                     IHETSKIFSIEIEPEKEI"
     gene            complement(3987..5474)
                     /locus_tag="PKU88_11470"
     CDS             complement(3987..5474)
                     /locus_tag="PKU88_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HPX69928.1"
                     /translation="MNIFSQKLREVLASVLPIVFIVLILNYTVLPIASNLIIRFLIGS
                     VLIIFGLTVFLVGVDIGVTPFGSLIGTSLTKNNKLWIIAVSGLLLGFFVSIAEPGLLV
                     LANQVALVTLGGISSSSIVIVVSVGLAVLVSFGFIRVVFNIPLYKVLTVSYLLIFILA
                     LFTSPEFLAIAFDASGATTGVLAVPFILALSLGIAVLNKDSKSAEKDSFGLVSIASIG
                     AIISVMILDILSKKNEYAVSLEFNILHSDSIFEPFFKLMPDILKESFIAILPLLVIFL
                     VFQRISFKLERRAYKRILIGFGYTFLGLAIFLIGVNAGFMELGSVIGYNLASFDNKWY
                     VVIIGFVIGLVTILAEPAVHVLTYQIEEVTAGYVKRSAVLVPLSLGVGIAIALSVIRI
                     LIPEIQLWHYLLPGYIISIVMTYFAPKLFVGIAFDAGGVATGPMTATFILAYIQGVAD
                     AIEWASVMRDGFGMISMVALSPIITLQILGLLFKMKSKKEVDKNA"
     gene            complement(5513..5902)
                     /locus_tag="PKU88_11475"
     CDS             complement(5513..5902)
                     /locus_tag="PKU88_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69929.1"
                     /translation="MKYIYNRKLFISVMFILLVICNVILPCSDFPILINFDKIPSIQF
                     HDETPSLKLDEENFNGVSIMLPRAQYKLKYNANSNSSIFEIIETSKFVLALQFALQLL
                     VFDRRKQIIALIISHFEGGKYKSSLSL"
     gene            complement(6107..7729)
                     /locus_tag="PKU88_11480"
     CDS             complement(6107..7729)
                     /locus_tag="PKU88_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69930.1"
                     /translation="MNVSYENIPERSNSYIGLYSIGETDEYWSDLQSTGNNTTGNVFF
                     DMPMIPGAYQFRSVYFTNGVYVRTGVSSPFYVMPHNTVFRNIPDKINPTGQLNIQIEN
                     APYGERTWAGIYPLNSNRYDTYWGNLVSLSNSNSGVINSTAPVNPGLYCVRVFSQGSN
                     IIYLSTSPSFEAAEGATAPQDNQSPVIGDLGKTDNINQVDYNVTTGHFEGQLSPLTLQ
                     AQAGNRHIKLNWTRVTDSRGVLGYNIYRSTSPGGQTEQPLNASLINGTLYTDISVNVD
                     TTYYYTVRPVYGDSSIGVRSNEVSAAPKKWAATMDTSVGIAPGTTTGTTPGTTTGTTT
                     GTTTGTGTSSQQQGDGQWNGVWKIDSGMMMLTQTGNSVTGTYGEWEDNLAINGTVSGN
                     RLTGTMDTGFGPGRFEFTIAQDGSISGWSRPDGYEAWEESFEWVGVKLSDSFGNTGNA
                     WGGVWYTDFGPMVLAQNGSGITGVYRPYCDVPVFQIEGTASGNAFTGRVREGEFTGEF
                     RFNLESGGRSFSGQFRYIGEDEWGDWNGIKKR"
     gene            complement(7726..7884)
                     /locus_tag="PKU88_11485"
     CDS             complement(7726..7884)
                     /locus_tag="PKU88_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69931.1"
                     /translation="MYKKIFSWILVLALFLQLLTPIGVQAENREYFIINKMEAAPGEP
                     YHPGRADR"
     gene            8237..9088
                     /locus_tag="PKU88_11490"
     CDS             8237..9088
                     /locus_tag="PKU88_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69932.1"
                     /translation="MSNVMWYVSLVGISIGIAAIAIYMKRDKYQFSTYMVFYLFSATI
                     TWIGEFIVLGLFNSYAYKTGISQNPWAQNLLGHLLLNTSMFPAAATVMIAYSLKIGGI
                     VLTAFLFLLPEYLFTKLGIYEQHWWNYYMSFFNVIAFMLISGKWFSKMYEERRGLTRA
                     VTYFFIAFVCIHFPSPILLLAGKQHYKLSFVRKIFDDYYLSSIIVSFTYHLVLCIVFV
                     YFVCILKKWYWKIVPFLTCITVLTIFEKTDILIIHNGWKFIYTILLQQISIAIFILIE
                     KFTLKPD"
     gene            complement(9097..>9997)
                     /locus_tag="PKU88_11495"
     CDS             complement(9097..>9997)
                     /locus_tag="PKU88_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX69933.1"
                     /translation="WSLIYFSVFAAIPVVIAYVLSARRDLGAGVLLARPGLSEAAPGL
                     SSPLALAWRLHKRSFMGWLAGAVLYIVVFSAFSPGLSKSGGISGWLFDLGGTDWTDEV
                     GLGYVFISIGIYLVSLFVAAYAITAVLRLKKEENEGRAEMLVDKQVSRIRWMSSHLII
                     AALCSAALLLATGIAGGLAYGLAADDLSNGFWRIFCMNVSKIPPVWILLGITALLYGL
                     WPQITALSWVVWVSFSMLELVWEAQIIDWSLMWISPFSYAHYTIDIANLPLLPLLGLL
                     CFSAILTGIGLIGFRNRDVSTKA"
BASE COUNT         2859 a         2325 c         1727 g         2986 t
ORIGIN      
        1 aaggaagtac ggctccgtac ccccttgtac cgatctgtca tcagctgttt caatcaaccc
       61 tgtatattct tttcatatct gcaactgaaa ccgagtgaag acatttgcag gaaaagcgaa
      121 atcagtacca acataattaa aatcctattg cgaatccttg atatattatt tataccttct
      181 ttcactaaaa atttcccgta tatcaaataa ttaactaaat tatagctccg atttccgcga
      241 aatgtcaata aagaggggca catgcccctc tcattccaat aacaaaatag cactttccgg
      301 aaatctcaga tacaaattgt ctttgtctct tatattgtcc cattcctgtt tatcagcttt
      361 atacagtatc tccccattat cattttcact attgctcttt ctgttttcaa aaaagacatt
      421 gtattcgaac acatcttcga ctatcctgtt aatcctgcac tccattacat ttttttcttt
      481 tgcctggcct gcatcagtga tttgaaggtc atgtgccctg ataccgacat atttaacctg
      541 ttgaggcact ttttgctccg tttccaatat tacatcccaa tcggcagcat aaacattttt
      601 gggggacaaa acctcgcatt ttgaaatatt tttgcacccg gtgagcctgg ctgcaccata
      661 caatttcggt tgtctgaata tatcctttgt gtccccgtgt aaaatggacc tcccgttatc
      721 aagcaccaca agcctgctgc aaaaactata tacttctccc ctgctgtgcg taaccatcaa
      781 aacttctccc tcatacaatt ttaacaattc caaaaccccg tcctgaagct gttctctcag
      841 gtgcgaatcc aatgcagaaa acggctcatc cagcatcaat atatccggtt tgtatacaat
      901 gcatcttgcc agtgcaaccc tttgctgctg tccccccgaa atcttgtttg gatacttctt
      961 ttccagtccc tgcaaatgaa aggacactat catttcatta accttttgtt tcttttcttt
     1021 ctccggaagg cgcaatccca tgccgatgtt ctcttctacc gtcatatgag gaaacagggc
     1081 ataattttga aataaatagc cgatattcct ttcctgcggc ttaagattga tcttcttttc
     1141 ggaatcatac aggactttac cgttaagaac aatacagcct tcatcagggg tttcaattcc
     1201 ggcaatacat ttcagcgtca agcttttccc gctgccggaa gcccccagga ttccaagata
     1261 ctccccctct gttctaaaag aaacgtcaag agaaaaacct ttcagttttt tctttatatc
     1321 aaccgccaag ctcatatgct tctcccctgt ttgattattt ccattacttc tacttgtttt
     1381 tcactatacc acttactttg ctttccccta taccgctact taaagtattt tctttccctt
     1441 atagaaaaat aatttgtcaa aacaacaaca acaaaggata ttaacataac aattattaca
     1501 taataagagg cagcctgcat attcccggca gcaacctccg aataaattgc caatggaagc
     1561 gtcctcgtct tattttctat gttacctgcg atcatagccg tagcgccaaa ctcgcccaag
     1621 cccctggcaa atgccaatat cccgcctccg gcaacacccg gtaaagcaag gggcattatt
     1681 atttcccaga aaatccttct ctcggacatt cccaatgtcc ttgctgccat aattaattct
     1741 tggtctacct gttcaaaggc acctctggca gaacggtaca taaggggaaa tgaaattacc
     1801 gcagctgcta ttacggtggc actccaggag aaaactattt ttgtacccaa aaattgcaga
     1861 aggaatttgc cgacaggcct gtttacacca aaaacaatca gaaggagaaa tcctgctact
     1921 gtcggcggca aaaccagagg taatgttaat atgccatcca atatcatctt tgctttgtcg
     1981 gatttcatcc ttacaatcca tcttgcaaca tatagccctg acaaaaatgt taaggctata
     2041 gatgcggaag cagttttcag tgagattatt atcggtgaca tttgcatatc atacactcct
     2101 acttgattgc tgtgaaacca tatgattcaa atactttctt tgcttcatcg gttttcaaga
     2161 actcaacaaa tttttttgat gcatccattt ctttagaagc tttaactatg cctgccggat
     2221 aaatcacttt agaaaccgtt ccttccggcg cttccgcagc aacttttact tttttgtttg
     2281 aagctgcatc ggttgaataa acgataccgg catcggcgct gccttcagct acccagttca
     2341 gtacttccgt aacatttgtt ccaaggcttg cctttttaga tatctcttcc caaatattaa
     2401 ggtttgttaa tgcttctttt gcatactggc ccgccggtac gcttgccggg tcgccgacgg
     2461 ctattttatc agcctttaaa atatcttcaa aagcggttat tccctttgtg ctgttttccg
     2521 gaactattag cacaatcttg ttttccaaaa gctgaacaat agagttttcc tcaattaagc
     2581 ctttttcatt caatgcatcc atttgcttca tagctgctga cataaacaca tctatggacg
     2641 caccttcttc aatttgtgtc tgtaattttc ccgagctgtc ataggttgcc aatacattga
     2701 taccgggatt tttttcttta aacatcggta tcacttcacc gtctatgcag tttttcaagc
     2761 ttgccgcagc agcaacggta atagttgatt cttttgcggc agcattgttg ctgcagcctg
     2821 tcgaagccaa tactatgcac aaggcaaaca acgcaatagc aactgttctt ttcatttttg
     2881 accctccttt tttgaaacca attaaaccta actaaaacta actcaaccta actaaaaata
     2941 gtttaccaga attaatccga tatgtaaaca ttctatgtaa tcatattgct ttatggtaaa
     3001 atggtattat gtaattatag aactgcaaag aaggtgagtt aattgaacga ggatgtctta
     3061 tatactccgg aggagattgc tctgaagctt aggataacaa aaggaacagt atatgaaatg
     3121 attaaaagag gtgagctgga ggctcatcga atagggaggt atattcgcat atctcaaact
     3181 caatttgaat tatacctgtt aaaggcaaaa ggctatgaaa atatatatga ggcagtcata
     3241 agccgggaaa gcggcggaac ctttgcaaat atcggatcgg ttaaaattta tgtcgatact
     3301 gctttggaag ggcaggttaa agtatctgtc cgcccggaaa atattatatt aagtatgggn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aatttctttt tccggttcta
     3481 tttctattga gaatatctta ctggtttcat ggatgccgga gcctcttgcg tggataattg
     3541 tccctcctct tgcccctgcg tcccgtgctg cttcaactgc agcttccgct ttgcctcttt
     3601 caactattgc cataataaga ttatacatgg gattctccgc acctccgctt acattgtcaa
     3661 tcttacagcc gcatctggca atgcccaata tactttttat tgaagtagta aatgcaatgc
     3721 cgtgattcgg ctttgaaaat ttaaacttcc tattaagctc ttcaagtaca gtatcttcta
     3781 tagctgcttc tgctgccatc agtacaattt ccttcctgct ctcggacagt tccaaaagct
     3841 ccagaatagc acttttcacc gttcctttcc caaggaaaat ggttcccccg gttaccccgt
     3901 gctgcttgga tagtttcaga acatcactgc ccaatccgaa atttacgatt acacagatta
     3961 gattatagtc tttattctca aggcaattat gcatttttat ctacctcctt tttggacttc
     4021 atcttaaata gcagccccaa tatttgaaga gtgataatgg gggacaaagc caccatggaa
     4081 atcattccga agccatccct cataacactt gcccattcaa tggcatccgc aacgccttga
     4141 atataagcca gtataaaagt tgcagtcata gggccggttg caacccctcc tgcatcaaag
     4201 gctatgccta caaagagctt tggtgcaaaa tatgtcatga ctatcgaaat tatatatccg
     4261 ggtaaaagat aatgccataa ctgaatctca ggaatcaata tccttattac tgataaagca
     4321 atggcaatgc ctactccaag agaaagcggc actaatacgg ctgatctttt aacataaccg
     4381 gcagttacct cttctatctg atacgtcaga acatgaactg ccggctcggc caatatcgtc
     4441 accagaccga tgacgaaacc gataattaca acataccact tattgtcaaa ggaagcaaga
     4501 ttatatccta tcacacttcc cagctccata aagcccgcat tgacaccgat cagaaatatt
     4561 gcaagcccca ggaatgtata accgaaccct attagtatcc ttttatatgc tctccgctcc
     4621 agcttgaagg atattctttg aaaaaccagg aatataacaa gcaagggtaa aattgcgata
     4681 aaactctctt tcagtatatc cggcatcaac ttgaaaaagg gctcaaaaat tgaatccgaa
     4741 tgtaatatat taaattcaag gcttacagca tactcattct tttttgacag tatatccaaa
     4801 atcattaccg aaattatagc accaatagat gctatggaaa ccaatccaaa actatctttt
     4861 tccgcagatt tgctatcctt gttcagaaca gctatcccca gggaaagcgc aaggataaag
     4921 ggtacggcaa gtactccggt ggtagcgcct gatgcatcaa aggctattgc taaaaactca
     4981 ggtgaagtaa ataatgccag tataaaaatt aataaatatg atacagtcaa taccttatac
     5041 agaggtatat taaaaacaac tctgataaaa ccaaatgaaa ccaataccgc cagcccgaca
     5101 gacaccacta taactatact gctgcttgat atcccgccta atgtgactaa agcgacctgg
     5161 ttagccagga caagcaatcc cggctccgca atcgacacaa aaaaaccgag aagcaaaccg
     5221 gaaacggcaa taatccatag cttattattt ttagtaaggc ttgtgcctat aaggctgcca
     5281 aaaggagtaa ctccaatatc cactccaacc agaaaaacag ttaatccaaa aattatcagt
     5341 actgaaccga ttaaaaatct aatgattaaa tttgatgcaa taggcaagac agtatagttt
     5401 agtatcaaca caataaaaac tattggaagc actgatgcca atacctctct aagcttttga
     5461 ctaaatatat tcacaggtac atcacttcct tttcattaac ttctcataag tgttacaagg
     5521 aaagtgagct tttgtatttt cctccttcaa agtggcttat tattaatgct attatctgtt
     5581 tccgtctgtc aaataccagc aactgcaagg caaattgcag tgctaacaca aattttgaag
     5641 tttcaattat ttcaaatata ctgctattgg aatttgcatt atatttcagc ttatactgcg
     5701 ctcttggcag cataatcgat acaccgttaa agttttcttc atccaatttg aggctcgggg
     5761 tttcatcgtg gaattgaatg ctcggtattt tatcgaagtt aattaatatg ggaaaatcgc
     5821 tgcaaggcag aattacatta caaataacca acaatataaa cattacagat ataaaaagtt
     5881 ttctgttata aatatacttc ataataaaat ccctcattat gcatatctaa tgaaatttgc
     5941 ttaaaagttt taataaacag tggtttatta aaaatattgt ttaatttgta acaatatctt
     6001 aatgaatctc acattatctc gactttataa tatcataaaa cctgacctaa tacaatcaag
     6061 gtcaaggtat ctataaaaaa atgcgtctct cgacgcattt ttttcactat ctcttcttta
     6121 tcccgttcca atccccccac tcatcttctc cgatgtagcg gaactgcccg ctgaaggacc
     6181 ttcctccgct ttcaaggttg aatcggaatt ctcctgtaaa ttctccttcc cttactctgc
     6241 ctgtaaaagc atttcctgaa gctgtccctt ctatctgaaa aacaggtaca tcgcaatatg
     6301 gcctatatac tcctgtgatg ccggagccgt tctgggccaa caccataggg ccgaagtccg
     6361 tgtaccatac tcctccccat gcattgccag tgtttccgaa ggaatcggat aatttaactc
     6421 ctacccattc gaaggattcc tcccaagctt cataaccgtc aggacggctc cagccgctta
     6481 tgcttccgtc ctgagcaatt gtaaattcaa acctgccggg accaaagccg gtgtccatgg
     6541 tacccgttag cctgtttccg cttacagtac cgtttatcgc caagttgtct tcccattcac
     6601 cgtaggtgcc ggttacactg tttcccgtct gtgtgagcat catcatgcca ctgtctattt
     6661 tccatacacc gttccactgg ccatccccct gctgctggct cgaagtgcct gtacctgttg
     6721 ttgtacctgt tgttgttcca gttgttgtac cgggtgttgt gcctgttgtt gttccaggcg
     6781 ctattccaac ggacgtatcc atagtagccg cccatttttt aggtgcggca gacacctcat
     6841 tggatcttac ccctatgctg ctgtcgccgt aaacgggcct gacggtatag taataagtcg
     6901 tatcgacgtt tacgctaatg tcggtatata aggtaccatt gattaatgaa gcgttaagag
     6961 gctgctctgt ctgccctccc gggcttgtcg atctgtagat attgtatccc aatactcccc
     7021 gtgaatcggt aactctggtc caattcagct taatatgcct gtttccggcc tgtgcctgca
     7081 gtgttagcgg agataactgg ccctcaaaat gccctgttgt gacattataa tcaacttgat
     7141 ttatattgtc tgttttaccc aaatccccta ttacagggct ctgattgtct tgcggtgcgg
     7201 tcgctccctc agccgcttca aaggaagggc tggttgaaag gtatataata ttgctgccct
     7261 gactgaatac ccttacgcag tacaaacccg gattaaccgg cgcagtgctg ttgataaccc
     7321 cgctgttgct gttgctcagc gaaaccagat ttccccaata ggtatcatat ctgttgctgt
     7381 tcagagggta tatccctgcc caggtacgct caccgtatgg tgcgttttca atttgtatgt
     7441 tcaattgccc tgttgggttt attttgtcag gtatgttcct aaatacggta ttatggggca
     7501 ttacataaaa cggagaactt acgcctgttc ttacatatac cccgtttgta aagtaaacgg
     7561 atctgaattg gtatgcccca ggtatcatag gcatgtcaaa aaatacattg ccggttgtat
     7621 tatttccggt actttgcagg tccgaccaat attcatcagt ctcaccaata ctgtaaagcc
     7681 caatataaga gttgctcctt tcaggtatgt tttcataact gacattcatc tgtccgcccg
     7741 gccagggtga tatggctccc caggcgctgc ctccatttta ttgattatga agtattccct
     7801 attctcagcc tgaactccta ttggtgttaa aagctgaaga aataacgcca ataccagaat
     7861 ccacgaaaat atttttttat acaaatttac ctccttcaaa tttctcgttt ctgcctgccc
     7921 cctttatccc tggggtatgt ggctgttttt cctgctgctg ttatttcata accatttatt
     7981 tcttgatcag acaatattat tatttatttt attagacgtt aaatcgtaat tataggtaaa
     8041 atttgatcaa aatcagtacc aaaaatgtta ttttgataaa tactcccaac ctgcagcaga
     8101 ttgaagggcc gggaattcag cccctaatct gaataattga tatccgcaaa tcaaataata
     8161 ttgcttaaca aggcacattt ctgcagaatt tcctttttta ataatgtgct gtatttacaa
     8221 gtaaagcagg tataaaatgt caaacgtaat gtggtatgtt tctttggtcg gcatcagcat
     8281 aggtattgca gccattgcca tatatatgaa aagagacaaa tatcaatttt caacatacat
     8341 ggtattttat ttattttcag caaccattac atggattggt gaattcatcg tacttggcct
     8401 gtttaattcc tatgcatata aaacgggtat aagtcaaaac ccctgggctc agaacctgct
     8461 cggccatttg cttctcaata caagcatgtt ccccgcggca gcaacagtaa tgatagcata
     8521 ttctttaaaa atcggcggaa ttgttttaac tgcattttta tttctgcttc cggaatactt
     8581 atttactaaa ctgggcatat atgaacagca ttggtggaac tattacatgt ccttcttcaa
     8641 tgttattgct tttatgctga tttccgggaa gtggttttcc aaaatgtatg aagaacgccg
     8701 cgggctaacc agggctgtaa catatttttt tattgcattt gtatgcatac actttccgtc
     8761 tccaatattg ctgctggcag gtaagcagca ttacaaacta agctttgtca ggaaaatatt
     8821 tgatgattat tatctgtcaa gcattattgt ttcatttaca taccatcttg tcttatgcat
     8881 agtatttgta tactttgtat gcatacttaa aaaatggtat tggaaaatcg taccttttct
     8941 cacttgcatc acagttctga caatatttga aaaaacagac atcttgatta ttcataacgg
     9001 ttggaaattc atttatacca tactattgca gcaaattagc atagcaatat ttattctgat
     9061 tgaaaaattc accttaaaac cggattaaag ctgaatttat gcttttgttg aaacatctct
     9121 gttcctgaat ccaatcaatc caatccccgt tagtatggca gaaaagcaaa gcagcccaag
     9181 tagcggaagc agcggtaaat ttgctatatc aatggtatag tgggcatatg agaagggaga
     9241 aatccacatc agtgaccaat caattatctg agcttcccat acaagctcca acatactaaa
     9301 cgatacccaa accacccagc tcagagctgt tatttgaggc cataaaccgt acaataaagc
     9361 tgttatgcct aacagtatcc atacaggcgg gatttttgaa acgttcatac aaaaaatacg
     9421 ccagaatcca ttgcttaaat cgtctgctgc gagtccataa gccagtcccc cggcaatacc
     9481 cgttgccagc agcaatgcag cagagcataa agctgcaata attaaatggc tgctcatcca
     9541 tcgaatccgg ctcacctgct tatccaccag catctccgct ctgccctcat tttcctcctt
     9601 tttcaggcgc aatacagctg ttatggcata ggcagccaca aaaagcgaca ccagatagat
     9661 gccgatacta ataaagacat atcccaagcc cacttcatcg gtccaatccg tacctcccaa
     9721 atcgaacagc cagccgctta tcccgccgga tttggataag ccgggagaga aggctgaaaa
     9781 cacaacaata tacaatactg ctcccgccag ccagcccata aagcttctct tatgcagcct
     9841 ccaggctaat gccaaagggc tggaaagacc gggagccgct tccgaaagcc ccgggcgggc
     9901 caaaaggaca cctgccccca gatccctgcg ggcagaaagc acatatgcaa taacgacagg
     9961 aatggccgca aaaacagaaa aatatatcag gctccag
//
LOCUS       DAOOAB010000071         9594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_8865_length_9594_cov_15.112276, whole genome shotgun sequence.
ACCESSION   DAOOAB010000071 DAOOAB010000000
VERSION     DAOOAB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8865_length_9594_cov_15.112276"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..546)
                     /locus_tag="PKU88_11500"
     CDS             complement(100..546)
                     /locus_tag="PKU88_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPX69934.1"
                     /translation="MSLKEVLQEDLKSAMREKDVIRKNVITMVRAGILQIEKDKKIEL
                     DDEGVIEVIAKGVKQRKDSIPDFEKGNRPDLVENINKEIGILMGYLPQQLTEEELNQI
                     VSDTIFEVGAQSMQDLGKVMSALMPKVKGKADGKLVNSIVRQHLQR"
     gene            complement(563..739)
                     /gene="rpsU"
                     /locus_tag="PKU88_11505"
     CDS             complement(563..739)
                     /gene="rpsU"
                     /locus_tag="PKU88_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPX69935.1"
                     /translation="MSEIRVGENETLESALRRFKKKCARAGVLAEVRKREHYESPSVK
                     RRKKSEAARKRKTK"
     gene            complement(918..1262)
                     /locus_tag="PKU88_11510"
     CDS             complement(918..1262)
                     /locus_tag="PKU88_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPX69936.1"
                     /translation="MKDCIFCRIIDGEIPCSMVYQDEKVLVFKDINPLAPVHVLIVSK
                     QHIESAQELNESSSEIAAHIFGKIPGIAKELGIAESGYRVVTNIGKDGGQAVPHLHFH
                     ILGGRKLENALG"
     gene            complement(1360..2661)
                     /gene="mtaB"
                     /locus_tag="PKU88_11515"
     CDS             complement(1360..2661)
                     /gene="mtaB"
                     /locus_tag="PKU88_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPX69937.1"
                     /translation="MKKAAFYTLGCKVNQYETEAMTESFENAGYEIVDYSEFADVYII
                     NTCTVTNMGDRKSRQIIRRAREKNPEALVAVVGCYSQIAPGEVLEIPEVSLVVGTDER
                     SRMVELVEYAMEKEEKLNMVNDIMKVKEFEEMSIKSYKSRTRAFLKIQEGCDQYCTYC
                     IIPYARGHIRSRKPDSIIAEVKELAENGFREIVLTGIHVASYGKDLGNTSLIDIIEKV
                     HEIDGIRRIRMSSVDPNVMTDGFIERLSRLPKICGHFHLSLQSGCDETLKRMNRKYTT
                     GEYRRVVKKLREVFADVAITTDLIVGFPGETEEEFQKTVDFVEEIAFSAMHVFKYSQR
                     TGTPAAKYENQIKPQVKDSRSKVITAIAQKNEEKFKKAFIGRSKPVLYEQPFDGQKSL
                     FEGLTDNYIRVVSESREDIKGKIIETVLAELKEDYMTGRIY"
     gene            complement(2673..3413)
                     /locus_tag="PKU88_11520"
     CDS             complement(2673..3413)
                     /locus_tag="PKU88_11520"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPX69938.1"
                     /translation="MHRFFVKRQSILKDRITLSGEDVQHISKVLRLKCGDRIVLCDGE
                     GTDYIAVIESMAKDSIGAIILEKEAAKGEPDIDTVLYQGIPKSAKMDVIIQKCTEMGI
                     KRIVPVLTSRTVVRLESEKDERKKAERWAKIAEEAAKQSGRGIIPVIGMPVTLDQALR
                     SAAGLDLVIIPYELETSLPVKEVLSKNKAGSIGFFIGPEGGFEPVEVEKAKEMGAVPV
                     TLGRRILRTETAGIAVLTSIMYEYDQMK"
     gene            complement(3415..4353)
                     /gene="prmA"
                     /locus_tag="PKU88_11525"
     CDS             complement(3415..4353)
                     /gene="prmA"
                     /locus_tag="PKU88_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPX69939.1"
                     /translation="MKYVELQVITTTEASDAVSEILYSEGASGVLIEDPNDFNALNKN
                     EKSWDYIEDELIELMGKDARVKGYFPFVEFNETVLKSIADRIEKLKGFGLNKGKGTVS
                     TREVSDEDWANAWKKYYKPVKIGERVVIKPTWEEHKVKADEVVIELDPGMAFGTGTHE
                     TTIMCVKLLEKYIKSGSTVFDVGCGSGILGIAAAKLGAEKVVCVDIDEVSCRVSRENA
                     EINKVDERVDVRCGNLLNVVSGKADVIIANIIADIIISFSEDAMSFLAKGGIFISSGI
                     ISDRRDEVLRKLRAEDFSILEVLEMGEWCAIAAEGK"
     gene            complement(4516..5694)
                     /gene="dnaJ"
                     /locus_tag="PKU88_11530"
     CDS             complement(4516..5694)
                     /gene="dnaJ"
                     /locus_tag="PKU88_11530"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPX69940.1"
                     /translation="MASKRDYYEVLGIDKSASEEDIKKAYRKLAKKYHPDMNQGDKSA
                     ESKFKELNEAYEVLSDKDKKARYDQFGHAGVDPNGFGGAGGFGGFGGFGGFGGSTGTG
                     GFEDIFDMFFGGTGGFGSTGSAGRKTGPQKGTDLKYELEIEFEEAAFGVKKDIHVTRN
                     ETCNDCKGTGAKAGTSPETCKACGGSGEVRFTQSTIFGRVVNVRTCDECHGEGKVIKE
                     PCPQCKGRGSIRRSRKITIDIPAGVDTGSVMPLRGEGEPGLRGGSNGDLYIYMRVKPH
                     KLFKRDGINLYSEMPISFTQAALGDEIEVNTLEGKMKYTIPEGTQTGTTFRIKNKGIP
                     SLKSKMRGDLYFTVKVNVPKKLSEKQKELLRQYAAISGDDMEDQGKSFFDKVKDAFGK
                     "
     gene            complement(5827..7641)
                     /gene="dnaK"
                     /locus_tag="PKU88_11535"
     CDS             complement(5827..7641)
                     /gene="dnaK"
                     /locus_tag="PKU88_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPX69941.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGGEPVVIPNAEGGRTTPSVVGFSK
                     TGERLAGQVAKRQAVTNPERTVISIKREMGSNFKVNIDDKSYTPQEISAMILTKLKQD
                     AEAYLGEKVTQAVITVPAYFSDSQRQATKDAGKIAGLEVLRIINEPTAASLAYGLDKE
                     ENQKILVFDLGGGTFDVSILELGDGVFEVLATNGNNRLGGDDFDQRVVNYLADEFKKD
                     NGIDLRNDRMAMQRLMEAAEKAKIELSGVLQTNINLPFITADASGPKHLDVTLTRAKF
                     DELTHDLVEMTMEPARKALADAGLTADKLSKVILVGGSIRIPAVQEAVKKLTGKEPYK
                     GINPDECVAIGAAIQAAVLTGEVKDVLLLDVTPLSLGIETLGGVFTKLIDRNTTIPTK
                     KSQIFSTAADGQNSVEIHVLQGEREMAAYNKTLGRFQLSGIPPAPRGVPQIEVTFDID
                     ANGIVHVSAKDLGTGNEQKVTITASTNLSDSDIEAAVKEAEKFAAEDKKRKEEVEVRN
                     NADSLVYQTEKNLKDLGDKLGSEDKARIEAELNNVKEALKGTDTEAIKAAAEELTKVF
                     YDISGKIYQANAGAQGPAGEAPQDDNTVNADYKVEDDK"
     gene            complement(7670..8251)
                     /gene="grpE"
                     /locus_tag="PKU88_11540"
     CDS             complement(7670..8251)
                     /gene="grpE"
                     /locus_tag="PKU88_11540"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPX69942.1"
                     /translation="MKHKKQKEEEKPQVNNTIEENTAEEASAQTGEAVPDQAAETDNK
                     EILELKKQSEEYYNRLLRMQADFENYKKRIAREREDMYNNVLETITAQLLPIVDNMER
                     AVEAFNKDKLDEQYISGVEMVCNQIIDILKKNGVKEIEAMDKEFDPNLHHAVMKVPGE
                     DEDENKIKEVFQKGYILGNKVIRPSLVKVSVKQ"
     gene            complement(8285..9316)
                     /gene="hrcA"
                     /locus_tag="PKU88_11545"
     CDS             complement(8285..9316)
                     /gene="hrcA"
                     /locus_tag="PKU88_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPX69943.1"
                     /translation="MDMGNRKRLILQAIIEDYINSAEPVGSRTISKKYLTGTSPATIR
                     NEMSDLEDMGYIEQPHTSAGRIPSDKGYRLYVDKIMKQDKINEAQREMIKREFVESLG
                     EIDRLIKHASKLLSQLTQYTSIVMAPQFRRTSIKQVQLLRIDRAVVLAVIVTNAGIIK
                     NSVLRLKQDIQPDALIRITSMINDKMAGLDVEEIEKFDFQDMLNNSLGHSEIIEQVVP
                     ELIQTLFYSDTSEVYHDGVSNILNLPEYSDINKARDFLNIMEERELLFKVLNDVREDV
                     SVSIGKENKLEQFKECSLITATYKINNKVIGSVGIIGPTRMEYSKAISVVECMTNNLS
                     EILTSILKR"
BASE COUNT         2213 a         2543 c         1586 g         3252 t
ORIGIN      
        1 aaaaaagaca tggggacgga agacatgggg acgtttcccc tgtcccactt ccagtgggac
       61 acgcgaaacg tccccatgtc ttccgtcccc atgtcttttt tatctctgta aatgctgtct
      121 tacaattgaa ttaaccaatt tgccgtcagc cttgcccttt accttgggca taagcgcact
      181 catcaccttg cctaaatcct gcatgctctg agcgcctact tcaaaaatcg tatctgaaac
      241 tatttggttg agttcttcct cggtaagctg ctgcggcaaa taacccatca gtataccaat
      301 ttctttatta atattttcaa ccaggtcggg cctgttgcct ttttcaaagt caggtatcga
      361 atccttcctt tgtttaactc ctttggcaat tacctctata acaccctcat catccagctc
      421 tattttcttg tccttttcaa tctgcaatat accggctctc accatggtta taacattttt
      481 ccttataaca tctttttctc tcattgcaga cttaaggtct tcctgtaata cttccttgag
      541 ggacattcac atcaaccttc ttttattttg tttttctttt tcttgcagct tcagatttct
      601 tccttctttt tacacttggg ctctcataat gctctctttt cctaacctct gccaaaaccc
      661 cagccctggc acatttcttt ttgaaccttc taagagcgct ttcaagagtt tcgttttcac
      721 caactctaat ttcagacata catttccctc cctccgctgg taacaatcct tacagtatat
      781 tgctatactg cgttactaag tgggacatat caaaaccata tcatatccat caatttactc
      841 ttctgaatac gataaaatac tcagtttatt atacattact tgattaatta gtgtcaataa
      901 cgggtttttg gctcaggcta tcccaaagca ttttcaagct tgcggccgcc caatatatga
      961 aagtgcagat gaggaactgc ctgcccgcca tcttttccga tattagtaac tactcggtag
     1021 ccgctttccg ctatacctaa ttcctttgct atacccggaa ttttgccgaa aatatgtgcc
     1081 gcaatctctg agctgctctc attcaattcc tgggcagact caatatgctg ctttgataca
     1141 atcaatacat gaaccggagc aagtgggttg atatctttaa aaaccaatac tttttcatcc
     1201 tgataaacca tgctgcaagg tatttctcca tctataattc tgcaaaaaat acagtctttc
     1261 atcacttcac ctccttagtt cagtctgatc ttgcaatata atacggcgta ctaaatgtta
     1321 ttcaacaaaa ttccgttaaa tcctgcacaa ataaataaat taatatattc ttccggtcat
     1381 ataatcttct tttaattccg caagaactgt ttcaattatt tttcccttta tgtcttcccg
     1441 tgattcagat acaaccctta tataattatc tgtcaagcct tcaaaaagag atttttgacc
     1501 atcaaacggc tgttcataca gaaccggctt gctgcgcccg ataaaggctt ttttgaattt
     1561 ttcttcattt ttttgggcaa ttgcggttat taccttgctt ctcgaatcct tcacctgcgg
     1621 ctttatctga ttctcatact ttgctgcagg agtgccggtt ctttgtgaat acttgaaaac
     1681 atgcatggcg ctgaaggcta tctcctcaac aaaatcaacc gttttttgaa actcctcttc
     1741 tgtctctccc ggaaatccca caatcaaatc cgttgtaatc gccacatcgg caaatacttc
     1801 tctcagcttt ttcactaccc ttctgtattc cccggttgtg tactttctgt tcatcctctt
     1861 aagggtctca tcacatccgc tttgaagaga aagatggaaa tgcccgcata ttttcggaag
     1921 gcggctgagc ctttcaatga atccgtcagt cattacatta ggatctacgg aactcatccg
     1981 aatgcgccgg attccgtcta tttcatgaac cttttcaatt atatcaatca aagaagtatt
     2041 ccccaggtcc tttccgtaag aagcaacatg tattcccgtc agcactatct ccctaaagcc
     2101 gttctccgcc aactctttta cttcggctat tatactgtcc ggtttgcggc tcctaatatg
     2161 ccccctggca tatggaatta tgcaatatgt acagtactgg tcgcagccct cctgtatttt
     2221 aagaaatgcc ctcgttctgc tcttgtagct ctttatggac atctcctcaa attcttttac
     2281 tttcataatg tcattaacca tattaagctt ttcttctttt tccattgcgt attcaaccaa
     2341 ttctaccatt ctgctgcgtt catccgtgcc gactaccagg cttacttccg gtatctcaag
     2401 cacttctcca ggtgctatct gcgaatagca acccacgaca gcaacaaggg cttcagggtt
     2461 cttttccctg gctcttctta taatctgtct ggatttcctg tctcccatat tggttactgt
     2521 gcaggtgttt attatatata catccgcaaa ttcggaataa tctacaatct cgtagcctgc
     2581 attctcaaag ctctctgtca ttgcttccgt ctcatactgg ttgaccttgc agccaagtgt
     2641 ataaaatgcc gcctttttca atggatttcc tcctacttca tctgatcata ctcgtacatt
     2701 atgcttgtga gtaccgcaat tcccgccgtt tccgtgcgaa gtatccttct gcccaacgtt
     2761 accggtactg ctcccatctc ttttgctttt tccacctcga caggctcgaa gcccccttcc
     2821 gggcctatga aaaagcctat gcttccggct ttgtttttgc ttaatacttc cttaacaggt
     2881 aggctagtct ccagttcgta gggtataatt acaagatcca gcccggctgc agaccttagg
     2941 gcctgatcca gagtcacagg catgcctata acagggatta tccctctgcc gctttgcttt
     3001 gcggcttctt cagctatctt agcccatctt tctgctttct tcctctcatc cttttcggat
     3061 tcaagcctta ctacagttct ggaagtaagt acgggaacga ttctcttaat acccatttca
     3121 gtgcactttt gaataataac atccattttg gctgatttgg gtatgccctg atacagtacc
     3181 gtatcaatat ccggctcccc ctttgctgct tccttttcca gtattattgc gcctattgaa
     3241 tccttagcca tgctttcaat aactgcaata taatcggtcc cttccccgtc gcacagcact
     3301 atcctgtcgc cgcactttaa acgaagcact ttagatatat gttgaacatc ttctccggaa
     3361 agggtaatcc tgtctttcaa aatgctctgc cttttaacaa agaatctatg catactattt
     3421 cccttctgcc gcaatggcgc accattctcc catctccagc acctcaagaa tactgaagtc
     3481 ttctgctctg agttttctca aaacctcatc ccttctatca cttatgatgc cggaggaaat
     3541 aaatatgcct ccttttgcta gaaaactcat tgcatcctca ctaaaggaaa tgattatatc
     3601 agctattata ttcgcaatta ttacatcagc cttgccggat accacattta ggaggttccc
     3661 gcatcttacg tccactcttt catcaacctt gttaatttct gcattttctc tgcttactct
     3721 gcaggaaacc tcgtcaatat ctacgcaaac aactttttca gcaccaagct tggctgctgc
     3781 tattccaagt attccggagc cgcagcccac gtcaaaaacc gtgctgccgc tcttgatata
     3841 cttttctaaa agcttcacgc acattattgt tgtttcatga gttcctgtac caaaagccat
     3901 tccggggtca agctcaatta ctacctcatc tgccttaact ttatgctcct cccaagtggg
     3961 tttgattact acccgctctc ctatcttcac cggcttgtaa tatttcttcc aagcgtttgc
     4021 ccagtcttca tcacttacct cccgggtgga gacagtgcct ttccccttat taaggccgaa
     4081 tcctttaagc ttttcaatgc gatcagctat ggatttcagt acggtttcgt taaattccac
     4141 gaaaggaaaa tatcccttta ccctggcatc ctttcccatc agttctatca actcatcttc
     4201 aatataatcc cagctttttt cattcttatt caatgcattg aaatcattcg gatcctctat
     4261 aagcaccccg cttgctccct cactatacaa tatctcggat accgcatccg atgcctcggt
     4321 agttgttatt acctgcagtt ctacgtactt caatctatca tcctcttaca ataaaatatt
     4381 caaggcttgt acttcatgtt acaattcgtc atctttacat tatatataat aagataaata
     4441 tttacaatat atgcacaaaa tcctgccgtt aaagtgccgg atacacagaa aagcccggca
     4501 tcaccgagct tttcgttatt ttccaaaagc atccttgacc ttgtcaaaga aggatttccc
     4561 ctggtcctcc atatcgtctc cgcttatcgc agcgtactgt cttagaagct ccttttgctt
     4621 ctcgctgagc ttctttggta catttacctt gactgtgaag tagagatctc ctctcatttt
     4681 gctcttcagg cttggtatgc ccttattttt tatcctgaaa gtagtacccg tctgagtacc
     4741 ttccggtatt gtatacttca tctttccctc aagggtattc acttctatct catcccccag
     4801 ggctgcctga gtaaaggaaa taggcatttc gctgtatagg tttatgccat ctctcttaaa
     4861 cagcttatgg ggtttcactc tcatgtaaat atacagatcg ccgttggaac cccctcttaa
     4921 acccggttct ccttctcctc tgagaggcat aacagaaccg gtatcgacac cggcaggtat
     4981 atcaatagta atctttctgc ttcttcttat gctgcccctg cccttacact gggggcaagg
     5041 ctccttaatg accttgcctt ccccatggca ctcgtcgcat gtccttacat tcacaactct
     5101 tccaaaaata gtgctctggg taaaccttac ttcgccgctt cctccgcatg ccttgcaggt
     5161 ctcggggctg gttccggctt ttgctcccgt gcctttgcag tcattacagg tctcattcct
     5221 tgttacatgt atatcctttt ttactccaaa agctgcttct tcaaactcta tctcaagctc
     5281 gtacttaaga tccgtaccct tctgagggcc ggttttcctc ccggcgcttc ccgtgctgcc
     5341 aaagcctccg gtgcccccga aaaacatgtc gaatatgtct tcaaagccgc cggtaccggt
     5401 tgatccgcca aagccgccaa agcctccgaa gccgccaaaa cctcccgcac cgccaaagcc
     5461 attcggatca actcccgcat ggccgaattg gtcgtatctg gcttttttgt ccttatcgct
     5521 caatacctca taagcttcat tcagttcttt aaatttactt tccgcacttt tgtcaccctg
     5581 gttcatatca ggatggtatt tctttgcaag ctttctgtat gcttttttta tatcttcttc
     5641 agaagctgac ttatctatgc ccaatacttc ataataatct ctttttgatg ccatttcttc
     5701 tccaccttta cataaaccag tctatgaata atgacgccat tctcgtaccc ataattatca
     5761 taactgagca ggaaactata aagtatcctg ctcagtacct ttgaaccgca ataattatat
     5821 cactaattac ttgtcgtctt caaccttata atctgcattt acagtattgt catcctgcgg
     5881 agcttccccg gcaggcccct gtgcccccgc attagcctga tatattttcc ccgatatgtc
     5941 atagaatacc tttgtaagtt cctcagcagc agcttttatt gcttccgtgt ctgttccctt
     6001 caaggcctcc ttgacattat tcaattcggc ttcaattctt gctttgtctt cactgccaag
     6061 tttgtctcca aggtccttaa ggttcttttc ggtctgatat accaaactgt ctgcattatt
     6121 ccttacttca acctcttctt ttctcttttt atcttctgcg gcaaactttt ctgcttcttt
     6181 taccgctgct tcgatatcgc tgtcagaaag attggtgctt gctgtaatcg tcaccttctg
     6241 ctcatttccg gtacccaggt cttttgcaga tacatgtacg ataccgttag catctatatc
     6301 aaaggttacc tctatctgag gcactcccct tggagccgga ggtatcccgc taagctggaa
     6361 ccttcccaat gtcttgttgt aagctgccat ttccctttcg ccctgcagta cgtgtatttc
     6421 aacgctgttc tgcccatcag cggctgtaga gaatatctgg ctcttttttg tcggtatggt
     6481 tgtatttctg tctataagct ttgtgaatac tccgcctaat gtctctattc caagtgaaag
     6541 aggagtaaca tcaagaagca gtacatcctt gacttctcct gtcagcactg cggcctgtat
     6601 cgcagcgcct attgcaacgc attcgtcagg gtttatcccc ttgtaaggtt cctttcctgt
     6661 aagcttcttc acggcttcct gaaccgcagg tattctaatt gaaccgccta ccaatattac
     6721 tttgctcaac ttgtctgctg tgaggcccgc gtctgccagg gctttccttg caggctccat
     6781 tgtcatctcc accagatcgt gggtaagctc gtcgaactta gcccttgtaa gggtaacgtc
     6841 aagatgcttc ggtccgcttg catcagcagt aataaacggt agatttatat ttgtctgaag
     6901 cactcccgaa agctcaatct tcgccttttc agcagcctcc ataagcctct gcattgccat
     6961 tctgtcattg cgcaggtcaa ttccgttatc cttcttgaac tcgtcggcaa gataatttac
     7021 tactctctgg tcgaagtcgt caccgccaag cctgttgttg ccgtttgttg ccaatacttc
     7081 aaacaccccg tctccaagct ccagtatcga tacatcgaag gtgccccctc ccaggtcgaa
     7141 caccagtatt ttctgatttt cttctttgtc aaggccatat gccagggatg ccgccgtcgg
     7201 ctcattgatt atcctgagca cttcaaggcc ggctatcttg cctgcatcct ttgttgcctg
     7261 cctctggctg tcgctgaaat atgcaggtac ggttataaca gcctgtgtaa ccttctcgcc
     7321 cagataagct tccgcatcct gtttaagctt tgtgagaatc attgcggata tttcctgagg
     7381 ggtatagctt ttatcatcaa tattcacctt gaaattagat cccatttccc tttttatcga
     7441 tattacagtt ctctccggat ttgtaactgc ctgccttttt gcaacctggc ctgcaagcct
     7501 ttctcctgtt tttgaaaatc ctacaaccga gggcgttgtt cttccgcctt cagcatttgg
     7561 aattactacc ggctctccgc cttccataac tgcgacacaa gagtttgtag ttcccaggtc
     7621 aatacctatt acttttgcca tttattgctt cctccttatt ttttagcact tattgcttca
     7681 ccgacacttt tacaagtgaa ggcctgatta ctttgtttcc cagaatgtat cctttttgaa
     7741 atacttcctt tattttattt tcatcctcat cctcaccggg caccttcatt acagcatggt
     7801 gaagattggg gtcaaattcc ttatccatgg cttcaatttc tttgacgcca ttctttttaa
     7861 gaatatctat gatctgattg cagaccattt ctactcctga gatgtattgt tcatccagct
     7921 tatccttatt gaacgcttcc actgcccttt ccatattgtc cacaatcggc agcagctgtg
     7981 cggttatagt ttcaaggacg ttattgtaca tatcttccct ttcccttgct atcctctttt
     8041 tatagttttc aaaatccgcc tgcattctta gaagcctgtt atagtattcc tcagactgct
     8101 ttttcagttc aagaatttct ttgttatcag tctccgccgc ctgatcagga actgcttctc
     8161 ctgtttgtgc agaagcttct tccgccgtat tctcttcaat cgtattatta acctgcggct
     8221 tttcctcttc tttttgtttt ttatgcttca tcaatctgac cacctttaag ttttatattt
     8281 aagattacct cttgagaatg ctcgtcaata tctcgctgag gttatttgtc atacactcaa
     8341 caacagaaat ggctttggaa tattccatcc ttgtggggcc aattatccca accgagccga
     8401 taaccttgtt attaatctta taagttgccg ttataaggct gcattccttg aactgctcaa
     8461 gcttgttttc cttgcctatg cttacactta catcctcacg aacatcattc agcactttaa
     8521 acagcagttc cctttcttcc attatattga ggaaatccct tgctttgttg atatcgctgt
     8581 attcaggaag attcaaaatg ttggaaaccc catcatggta cacttctgaa gtgtcggaat
     8641 aaaataatgt ctgtataagc tcaggtacca cttgttcaat tatttcactg tgtccaagag
     8701 aattgttcag catgtcctga aagtcaaatt tttctatttc ctctacatcc agcccagcca
     8761 tcttgtcatt aatcatactg gttattctga tgagggcatc cggctgtata tcctgcttca
     8821 atcttagaac actgttcttt attatccccg cattcgttac tattaccgcc aatactaccg
     8881 ctctgtctat tctgagaagc tgcacctgct taatgctggt tctgcgaaac tgaggagcca
     8941 tcactatgga tgtatattga gtcagctgtg agagcagctt tgaggcatgc tttataagcc
     9001 ggtcaatctc tcccagggat tcaacgaact ccctctttat catttccctt tgagcctcat
     9061 taattttatc ctgcttcata atcttatcta cgtataatct gtagcctttg tctgagggaa
     9121 ttcttccggc tgatgtatgg ggttgttcaa tatatcccat atcctcaaga tcagacatct
     9181 cgtttcttat tgttgcagga ctggtacccg tcaggtactt cttggatatt gtccttgatc
     9241 cgacaggttc tgcggagttt atataatctt caataatagc ttgcagtata agtcttttcc
     9301 tgtttcccat atccatcaat atcaccgccc ttgttagcac tctaccgtat tgagtgctaa
     9361 ttctgtcttt aaaataacat tgtcacatat ttttgtcaat aggttttaca acttttttct
     9421 gcttaataga agaatgatac gtcgtaaata taagcttgca gtcatactcc cagagcaata
     9481 gccttgtata ccgactcaca cgatattagc tttggacaga atcagagctt gatacttgca
     9541 aaatcctgac gcacataaac ccaacctcct gttggtatgt gctggattta cctc
//
LOCUS       DAOOAB010000072         9550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_8923_length_9550_cov_18.238231, whole genome shotgun sequence.
ACCESSION   DAOOAB010000072 DAOOAB010000000
VERSION     DAOOAB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8923_length_9550_cov_18.238231"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..701
                     /locus_tag="PKU88_11550"
     CDS             9..701
                     /locus_tag="PKU88_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175479.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPX69944.1"
                     /translation="MLSAGLIISTYIVTDGIVEIKGNRTITVTGSAKQQIKSDVIEWT
                     GSFSCQSKLMPEAYAKLKSDSELVKKYLKEKGINEEDMVLSSIDTNPIYEVNYNGQYT
                     NNIIAYRLYQRVSISSADVDKLSRIAREATELINEGIEFQSFSPQYYYSKIADLKVSM
                     LGEATKDAKNRAEQISQNTGSSIGALKSAKMGVFQITPLYSTEVSDYGINDTSSLEKE
                     ITAVVTCTFEIK"
     gene            732..2252
                     /locus_tag="PKU88_11555"
     CDS             732..2252
                     /locus_tag="PKU88_11555"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPX69945.1"
                     /translation="MKEFNDAKNGFFELVKRIKYYNDAIGVLFWDLKTGAPKKGVPYR
                     AEAIGMLSEEAFRLSTSDAMGEYLAYFDEPDRSSSLDSIMKALVRECRKEYDKYKKIP
                     EVKYKEYVILTSEAECVWEDAKKNNDFELFKPYLEKIVGYNLEFIELWGYKENKYDTL
                     LDLYEPGMTVEKLDAIFSELRSRIVPLVAKIKESAYQPEDQFLKQYFDIGKQEEFGLY
                     ILGRMGYDFDAGRLDESEHPFTTGINPGDVRITTHYYPNDFISAVMSSMHEGGHALYE
                     QNISADLYGTLLCDGASMGLHESQSRFWENTIGRSRSFWQKYYSRLQDFFPEQLKDVT
                     ADDFYRAINKVEPSLIRIEADEVTYNLHIMIRYEIEKALINEEIKVADLPGVWKEKMK
                     EYLGIIPPDDTKGVLQDVHWAGGSFGYFPSYTLGNIYSAQIYNAAKKEIKNFEELVEK
                     GELIKIKDWLAEKIHKHGKLLKPEEILLQVAGEGINPKYLIDYLENKYKEIYKLEF"
     gene            2532..3698
                     /gene="hisZ"
                     /locus_tag="PKU88_11560"
     CDS             2532..3698
                     /gene="hisZ"
                     /locus_tag="PKU88_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPX69946.1"
                     /translation="MQSFEVPQGMRDILTEESSRRTILQERLQHYMHSCGFGRVETPL
                     FEYYELFSGGISPVDDESIVKTIDRNGRVVVLRPDMTIPTARVASTNLKGQRKPLKLF
                     YAGSVYRADKKNRGAGREFCQVGAEIYGCSSKWLDIETLTMAKESFRVAGIEDYKIDI
                     GHVGIIKGIFEEMSLTEEKKSQIISLISEKNLVELENEVSALPLDSSSKEIICRLPCL
                     FGKPEDVFKGMDEIIVNETVKESVEYLLQIYGKCKDLGLGSNIIIDAGMTGNMKYYTG
                     LIFKAYARGAGDVVISGGRYDGLLKEMGSDAAASGFAIYIDNMLEAVTEKFEEQKGSK
                     ILVIFSESRFVEALKYSEGRRNNDAAVNMVNRGDISDPEQYCRQYGCNEVVIFE"
     gene            3711..4367
                     /gene="hisG"
                     /locus_tag="PKU88_11565"
     CDS             3711..4367
                     /gene="hisG"
                     /locus_tag="PKU88_11565"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPX69947.1"
                     /translation="MLKIAVAKGRIAEKVSELLLYTSDYRDIIDLNSRKLVFVDKNKE
                     IVFFLAKPSDVPVYVGSGAADIGIVGKDVLMEAKDSAYEIMDLKTCRCRIVLAGPSGK
                     SGVRPIVRKVATKYPRIASEYFNRKSESVEIIKLDGSIELAPIVGLSDAIVDIVETGN
                     TLRENGLVVYENICDITARMIVNRVSYKMKNKSINIFASAVSEAIEGGGANAQSAVMG
                     "
     gene            4342..5637
                     /gene="hisD"
                     /locus_tag="PKU88_11570"
     CDS             4342..5637
                     /gene="hisD"
                     /locus_tag="PKU88_11570"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPX69948.1"
                     /translation="MLRVLSWDDKSSIEGFLGQKNNGFIPEEVTETVKKIVRDVRDRG
                     DSAVLEYSRRFDNVNDIKDFRISSRALEKLAGEAEAGFIEAIGKSVDNIKRFHINMAD
                     RDFSIFTDNGSVLGSRVTPIDKAAIYVPGGKASYPSTVVMCAVPAMIAGVREIIILTP
                     PSDNGSINPYVAATAKLLGIDKVFRIGGAQGIAAAAFGTAAIPKVDKIVGPGNTYVAA
                     AKREVYGFVDIDMIAGPSEIAVVADSSANPGWVAADLLSQAEHDEMARCFLITDSGSF
                     AARVNTEIERQLGQMKRKGIISRALENNSAVIVTDAIEQAADIINRIAPEHLEIITEN
                     PFGVLKDIRNAGAIFLGAYSTEPIGDYIAGPSHVLPTYGTAAFFSPLSTRDFQKRSSL
                     IYYSKEDLAAYGPAAIKIAEAEGLEAHANALKVRLQNDR"
     gene            5627..6691
                     /gene="hisC"
                     /locus_tag="PKU88_11575"
     CDS             5627..6691
                     /gene="hisC"
                     /locus_tag="PKU88_11575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPX69949.1"
                     /translation="MIDNLVSKRVQELKSYKAESRIQGIALDKNELPWGLDDKVEEAL
                     IKSIKAMKFNRYPDSDYTELKAAISRYAGIEKQSICVGNGSDELISLVLQAFINPGDT
                     IAIYNPTFAMYRIYGSICGARIWKYDLDGNFELKPDEFIASLQEQKPKLVFLCNPNNP
                     TGKELALSEMEEILKNTAGIVVVDEAYFEFSGVTVLGFLSGYDNLIILRTFSKAFGLA
                     GLRIGYMLANPLIISYIDRVRSPFNVNAFAQAAAVEVLKNLDKVMERVELIKSERERL
                     IVLLKQLKGIQCFESKSNFILMRSPYSDEIAERLQKAGIFIRKYSSTKLKDCLRVTVG
                     SRGENDKFYETVKEVLYERA"
     gene            6678..7277
                     /gene="hisB"
                     /locus_tag="PKU88_11580"
     CDS             6678..7277
                     /gene="hisB"
                     /locus_tag="PKU88_11580"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPX69950.1"
                     /translation="MKELEGARTAEISRKTDETDVYVSLNLEGNGTYEINTGIPFFDH
                     MLEQLGKHSGFDLVVKAQGDIQVDSHHTVEDVGIVLGQALYQCTRNKRGIARYGSIML
                     PMDDSLCTAAVDFCGRANLVYNCILLQGSVGGFDTENIKEFFKAFVGNSAITLHINLM
                     YGENAHHNAECIFKAAARVLKEALRLDSRQGMPSTKGCL"
     gene            7281..7889
                     /gene="hisH"
                     /locus_tag="PKU88_11585"
     CDS             7281..7889
                     /gene="hisH"
                     /locus_tag="PKU88_11585"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPX69951.1"
                     /translation="MKLGIVDYGTGNIYSLQKALKHIGIDTVVSKDAIVLDCCSGIVL
                     PGVGAFCDAAANMSAYGLEQVILNQTAKGKLVLGICLGMQLMYDYSMEGGMHKGLGLI
                     SGRVERIPDTAKVPHMGWNTLSIQKKSNVLEGIGDGDYVYYVHSYYVKPEDSTYVCAC
                     SSYGVDIPAVVVKENIVGMQFHPEKSGETGLKILNNLKELII"
     gene            7886..8602
                     /gene="hisA"
                     /locus_tag="PKU88_11590"
     CDS             7886..8602
                     /gene="hisA"
                     /locus_tag="PKU88_11590"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPX69952.1"
                     /translation="MRIYPAVDIYDGKCVRLKEGKLAKMEMFYENPLDAAKMWEQKGA
                     KALHIIDLNGAFDGKTGNSKVIEKIIRSLSIPVQVGGGLRNVSGIKEAFNTGAARVII
                     GTAAVLDEELLETGLKKYIDKMIVSIDAKDGNVAIKGWVNVSEFSAYNFASKIVGKGF
                     RNIIYTDISRDGLLKGPNIDGIEKMCNVEGAKITASGGITSIDDILAIKEAGAEGAII
                     GKALYAGVLKLEDAIKAAED"
     gene            8604..9362
                     /gene="hisF"
                     /locus_tag="PKU88_11595"
     CDS             8604..9362
                     /gene="hisF"
                     /locus_tag="PKU88_11595"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPX69953.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEAARMYNAAGA
                     DELVFLDITASAESRGTMVDAVRETARDVFIPLTVGGGIDSLEDIDRLLHAGADKVSL
                     NTAAVNNPKLVYEGAKRYGSQCIVVAVDAKFKPGTAGWEVYIRGGRTATGIDVLEWCK
                     TVNELGAGEILLTSIDADGSKKGYDIALTSKVAETVNIPVIASGGAGSREDFLEVLTK
                     GKADAALAASLFHFGELRIGDLKLYLASKGVDVK"
BASE COUNT         2975 a         1732 c         2581 g         2262 t
ORIGIN      
        1 tggcccttat cttatcagca ggcttgataa tatcaaccta tatagttacc gatggtatag
       61 tggagataaa gggcaacaga accataacgg ttaccggctc ggcgaagcag cagataaaat
      121 cagatgttat agaatggaca gggagctttt cctgccagtc gaaacttatg cctgaagcgt
      181 atgcaaagct caagtcggat tcggagctgg taaagaaata cttgaaggaa aagggtataa
      241 acgaagagga tatggtttta tcctccatag atacaaatcc tatttatgaa gtaaattaca
      301 acggacagta taccaataac attattgcct accgattgta tcagcgggtg tcgatcagct
      361 ctgcggatgt tgataagctg agccgcatag caagggaggc aacggagctt attaacgaag
      421 gcatagaatt tcagtccttt tctcctcagt actactacag caaaattgca gacctgaagg
      481 tcagcatgct tggagaggca acaaaggatg caaagaacag ggccgagcag atatcccaga
      541 atacgggcag cagcatcgga gccttaaaat ctgcaaagat gggggtgttc cagatcacac
      601 cgttatactc cacagaagta tcggattatg gaataaacga tacatcttca ctggaaaagg
      661 agattacggc agtagtaacc tgtacctttg aaataaaata ggggttattt atatgagagg
      721 ggtagattaa tattaaagag tttaacgatg cgaaaaatgg tttttttgaa ctggtaaaaa
      781 ggataaaata ctataatgat gccattggtg tgcttttttg ggacctcaaa acaggagccc
      841 caaaaaaagg cgtgccctac agagcggaag caataggcat gctttctgag gaagccttca
      901 ggctgtctac ctctgatgcg atgggggaat acctggcgta ctttgatgag ccggatagaa
      961 gcagcagcct ggattcaatc atgaaggcac tggtgaggga gtgcaggaag gaatacgaca
     1021 aatacaaaaa gataccggaa gtaaaatata aggaatatgt gatattgacc tctgaagcgg
     1081 aatgcgtatg ggaggatgca aaaaagaata atgactttga gctgttcaag ccctacctgg
     1141 aaaagatagt cggctataac ctggagttca ttgagctgtg ggggtataag gagaataaat
     1201 acgatacatt gctggatttg tatgagcccg gaatgacggt tgaaaaactg gatgcaatat
     1261 tcagcgaatt gagaagcaga atagttcccc tggttgcaaa gataaaggaa tcggcttatc
     1321 agccggaaga tcagtttttg aagcagtatt tcgatatagg aaagcaagaa gaattcggac
     1381 tgtatatctt aggaaggatg ggttatgact ttgatgcagg caggctggat gagagtgagc
     1441 acccttttac aaccggaatt aatcccggag atgtgagaat aactacccat tactatccca
     1501 atgacttcat aagcgctgtc atgagttcaa tgcatgaagg agggcatgcc ttatatgagc
     1561 agaatatttc cgcagacttg tacggtactc tcctttgcga cggagcctcc atgggacttc
     1621 atgaatcaca atcaagattt tgggaaaaca ccataggaag gagccgcagc ttctggcaaa
     1681 aatactacag ccggctgcag gattttttcc cggagcagtt gaaggatgta accgccgatg
     1741 atttttacag ggccataaac aaggtagagc cgtcacttat cagaatagag gccgatgaag
     1801 tcacatataa ccttcatatc atgataaggt atgaaataga gaaagccctt attaacgaag
     1861 agattaaggt ggctgacctt cctggggtat ggaaggagaa gatgaaggaa tacctcggta
     1921 taataccccc cgacgataca aagggagtgc tgcaggatgt ccactgggcc ggcggaagct
     1981 tcggctattt tccctcctat actctgggga atatatactc cgcgcagatt tacaatgcgg
     2041 caaaaaaaga aatcaagaat tttgaagaat tggttgagaa gggtgaactg attaagataa
     2101 aagactggct ggcagaaaag atacataagc acggcaagct gctgaagcct gaagaaatcc
     2161 ttctgcaggt tgccggcgag ggaataaacc ccaagtattt gattgattac cttgaaaata
     2221 aatacaagga aatttataaa cttgaatttt agtattgaca acagggcttg ttttatatta
     2281 tagtatatga aaaccaattt acataataat tgcggagctg attttatgaa ttccttaaat
     2341 tatttgatta acagaatgca tcatcatagg cggagcactt aagcctttca ggaaatttcc
     2401 cgtaggcata agtgctattt gtgcttgtgc ctatgggatg taataataaa accccgtagg
     2461 taataaaaat aatacctgcg gggttttgtt attttcatga acaatacatt aggcaaggag
     2521 agatattatc tatgcaaagc tttgaggttc cacaaggcat gagggatatt cttactgaag
     2581 aaagcagcag gagaaccata cttcaggaaa ggctgcagca ttacatgcat tcctgcggct
     2641 tcggccgtgt agaaacacct ctttttgagt attatgagct gttttccggg ggcatttccc
     2701 cggtggatga tgaaagcatt gtaaaaacaa tagacaggaa tgggagagtt gtggttctta
     2761 ggccggatat gacaattccc acagccagag tagcatccac caacctgaag ggacagcgga
     2821 agcccttgaa gctgttttat gcgggaagtg tatacagggc ggacaagaag aaccggggag
     2881 caggaagaga attttgccag gtaggggcgg agatatacgg atgctcaagc aagtggctgg
     2941 acatagagac cttgacaatg gcaaaggaaa gcttcagagt tgcgggaatt gaggattaca
     3001 agatagatat agggcatgta ggcataatca aaggtatttt tgaagaaatg agcttgacgg
     3061 aagaaaagaa atcacagatt atctcactga taagtgaaaa gaatctggta gagcttgaaa
     3121 acgaggtatc cgcactgccc cttgacagca gcagcaagga aataatctgc aggctgccgt
     3181 gcctgttcgg aaaacctgag gatgtgttca agggtatgga tgaaattata gtcaatgaga
     3241 cggtaaagga atccgttgag tatctgctgc agatatacgg gaaatgcaag gaccttggcc
     3301 ttggctccaa tataataata gatgcgggaa tgacggggaa tatgaagtat tataccggat
     3361 tgatcttcaa ggcatatgcc cggggagccg gtgatgtggt tatttcggga ggaagatatg
     3421 acggtttgct gaaggaaatg ggttcggatg cggctgcatc agggtttgcc atatatattg
     3481 acaacatgct ggaagctgtc acggaaaagt ttgaggaaca gaaggggagc aaaatactgg
     3541 taatattcag cgaaagcagg tttgttgagg ctttgaagta ttccgaaggc cgcagaaaca
     3601 acgatgccgc agtaaatatg gtaaacagag gagacatatc ggatccggag caatactgca
     3661 gacagtatgg ctgcaacgaa gtggtaatat ttgagtgagg agtgtcgaaa atgttgaaaa
     3721 tagccgttgc aaaaggcagg atcgccgaaa aggtaagcga actgctgctt tatacaagtg
     3781 attacaggga tataatcgat ctgaattcca gaaagcttgt ttttgttgat aaaaacaaag
     3841 aaatagtatt tttcctggca aagccctccg atgttcccgt gtatgtggga agcggagcgg
     3901 cagatatagg aatagtaggc aaggatgtac ttatggaagc caaagattca gcctatgaaa
     3961 taatggactt gaagacctgc agatgcagaa tagtgctggc aggcccttcc gggaaaagcg
     4021 gcgtgaggcc tatagtaaga aaagtcgcta ccaaatatcc gagaatagca tcggaatatt
     4081 tcaacagaaa gtcggaatcc gtagagataa tcaagctgga cggttcaata gagctcgctc
     4141 ccattgtagg attgtccgat gcaatagttg acatagtcga aaccggaaat accctgaggg
     4201 aaaacggact tgtcgtatat gagaatattt gtgatataac tgcaaggatg atagtgaaca
     4261 gggtcagcta caagatgaaa aacaagagca taaatatatt tgcatcggct gtcagtgaag
     4321 ctatcgaagg gggtggggcc aatgctcaga gtgctgtcat gggatgacaa aagcagcatt
     4381 gagggttttt tggggcagaa gaacaatggt ttcattccgg aggaagtaac ggagacagta
     4441 aaaaaaatag tccgggatgt cagggatcgt ggagacagtg cagttcttga atacagcaga
     4501 agattcgata acgttaacga tataaaagac ttcagaataa gcagccgggc tctggaaaaa
     4561 ctggccgggg aagcggaggc cggcttcatt gaggctatag gcaagagtgt agacaatata
     4621 aaaaggtttc atattaatat ggccgacagg gacttcagca tatttaccga taacggcagt
     4681 gtgctgggca gcagggttac tcccatagac aaggctgcaa tatatgttcc cggaggcaaa
     4741 gcctcctatc cctccactgt tgtaatgtgt gcagtccccg caatgatagc cggagtgcgg
     4801 gaaataatca tattgactcc tccgtcggac aatggaagca tcaatcctta tgtagcggca
     4861 acggcaaagc ttctgggaat cgacaaggtg ttcagaatag gcggggccca gggaatcgcc
     4921 gcagctgctt ttggaaccgc agccattcct aaggtggaca agatagtagg gccggggaac
     4981 acatatgtgg cggctgcaaa aagagaagta tacggattcg tggatattga tatgatagca
     5041 gggcccagcg aaattgcggt agtcgcagac agcagcgcaa atccgggctg ggttgccgcc
     5101 gatctgctgt cccaggcaga gcatgacgaa atggcaaggt gctttctgat taccgattcc
     5161 gggagctttg cagcccgggt aaataccgaa atagaaaggc agcttggaca aatgaaaaga
     5221 aaaggaatta taagcagggc cttggaaaat aactcggcgg ttattgtgac agacgccata
     5281 gaacaggcgg cggatataat aaacagaatt gctccggaac acctggagat tataacggaa
     5341 aatccctttg gcgtgctgaa ggatataaga aatgcagggg caattttttt gggagcctat
     5401 tccacagagc ccataggaga ttacattgca ggccccagtc atgtactgcc cacttatggc
     5461 acggctgctt tcttctcccc cctttcaaca agggattttc agaaaaggtc aagcctgata
     5521 tattacagca aagaagattt ggcggcatat ggccctgccg caataaaaat cgctgaagcg
     5581 gaaggcctgg aggcccatgc caacgcactg aaagtgaggc ttcaaaatga tagataattt
     5641 ggtttcgaaa agagttcagg agcttaaaag ctataaagcg gaatccagga tacagggtat
     5701 agccctggat aaaaacgaat tgccctgggg cttggatgac aaggttgaag aggcgctgat
     5761 aaaaagcatt aaagccatga aattcaaccg ctaccccgat agtgactata cagagcttaa
     5821 agctgcaatt tcaaggtatg caggcattga aaagcagtca atatgcgtag gcaacggctc
     5881 cgacgagctt ataagcctgg tgctccaggc ctttattaac cccggcgaca ctatagcaat
     5941 ctataatccg acctttgcaa tgtatagaat atacggaagc atctgcggcg ccaggatatg
     6001 gaaatatgac ctggacggta attttgaact gaagccggat gaattcattg cttcacttca
     6061 ggaacaaaag cccaagcttg tatttttgtg caaccccaac aatcctaccg gcaaagagct
     6121 tgcactgtca gagatggaag aaatattaaa aaatactgca ggtatcgtag ttgtggatga
     6181 agcatacttt gaattttcag gagttacggt tttagggttt ttatccggat atgacaacct
     6241 tataatactc aggacctttt caaaggcttt cgggctggca gggttaagaa taggatatat
     6301 gctggcaaac cccttaatta tcagctatat cgacagagtc cggtctcctt tcaatgtgaa
     6361 cgcatttgcg caggctgcgg cggtggaggt tctgaaaaac cttgataaag tgatggaaag
     6421 agttgaactg ataaaaagcg agagggaaag gcttatagta ttgcttaagc agcttaaagg
     6481 catccaatgc tttgaatcaa agagtaattt catactgatg cgctccccct attccgatga
     6541 aatagccgag aggcttcaaa aggcagggat ttttattagg aaatactcaa gcacgaagct
     6601 taaggattgc ctgagagtta cagtcggcag ccggggcgaa aatgacaaat tctatgagac
     6661 agtaaaggag gtgctgtatg aaagagcttg aaggcgcaag gacagccgaa atatccagaa
     6721 agactgatga aacggatgta tatgtaagct tgaatcttga aggaaacggg acatatgaaa
     6781 taaataccgg aatacctttc tttgatcaca tgctggaaca gctgggaaag cacagcggct
     6841 tcgaccttgt tgtgaaagca cagggagata tacaggtgga ttcacaccat accgttgagg
     6901 atgtggggat agtactgggg caggcgctgt accaatgcac ccggaataaa aggggcattg
     6961 caagatacgg cagtataatg cttcctatgg atgactccct ttgtactgcg gcagtggatt
     7021 tttgcggaag ggcgaacctt gtatacaact gcatcctgct gcaaggctct gtaggaggat
     7081 ttgacactga gaacataaag gaatttttta aggcttttgt cggcaattcc gcaatcaccc
     7141 tgcatataaa cctgatgtac ggtgaaaacg cccaccataa tgcggaatgc atattcaagg
     7201 ctgctgccag ggtattaaag gaagctctgc gcctggacag caggcagggc atgccttcca
     7261 caaaggggtg tttgtaagat atgaaattgg gtattgtgga ttacggaacc ggcaacattt
     7321 acagtcttca aaaggccttg aagcatatcg gaattgacac tgttgtctca aaggatgcca
     7381 tagtccttga ctgctgttcc gggatcgtcc ttccgggggt aggagccttt tgtgatgctg
     7441 ctgccaatat gtcagcctat gggctggagc aggtaatatt gaatcagacg gccaaaggca
     7501 agctggttct gggaatatgc ctgggtatgc agcttatgta tgattacagc atggaaggcg
     7561 ggatgcacaa agggttaggg ctgatatccg gccgggttga aaggataccg gacacagcaa
     7621 aggttcctca tatggggtgg aacaccttga gcattcagaa aaaaagcaat gtccttgaag
     7681 gaataggaga cggtgattat gtatattacg tccactccta ttatgtaaaa cccgaagaca
     7741 gcacctatgt gtgcgcttgc agcagctacg gggttgacat accggcagtt gtcgtgaaag
     7801 aaaatattgt cgggatgcag tttcatcccg aaaaaagcgg agagacgggc ttgaagatat
     7861 tgaataattt gaaggagctg ataatatgag aatttatccg gcagttgata tttatgacgg
     7921 aaagtgcgta aggctgaaag agggaaaact tgcaaaaatg gaaatgtttt atgaaaatcc
     7981 tttggatgcg gcaaagatgt gggagcagaa gggtgcaaaa gctttgcata tcatagacct
     8041 gaacggtgcc tttgacggta aaacggggaa cagcaaggta attgagaaaa tcataaggtc
     8101 gttgagcata ccggtgcagg ttggaggcgg acttaggaat gtaagcggca taaaagaggc
     8161 tttcaatacg ggagcggcaa gagtgattat agggactgcc gcggtgctgg atgaagaact
     8221 tctggaaaca ggactgaaga agtacattga caaaatgata gtgagcattg atgccaagga
     8281 tggaaacgta gcaataaagg gatgggtaaa tgtaagcgaa ttctccgctt acaactttgc
     8341 aagcaaaatt gtagggaaag gttttagaaa tattatctat accgacattt ccagggatgg
     8401 cctgttaaag ggccccaata tcgacggaat agaaaaaatg tgcaatgttg agggtgcaaa
     8461 gataactgca tcagggggca tcacaagcat tgatgatatt ctggctatta aggaagcagg
     8521 agcagaggga gctattattg gtaaagcttt atatgcggga gttcttaagc tggaagatgc
     8581 cattaaggct gcggaggatt aatatgcttg caaaaaggat aataccgtgt ctggatgtaa
     8641 aggacggaag agttgtcaaa ggagtgagtt tcgtcaacct gagggatgcg ggggaccctg
     8701 tggaggcggc aaggatgtat aacgccgcag gggcggacga gctggtcttt cttgatatta
     8761 ccgcttccgc agaaagcagg ggcactatgg ttgacgcagt cagggagact gccagggatg
     8821 tgttcatacc ccttacggta ggagggggaa tagacagcct ggaggatatt gacaggcttc
     8881 ttcatgccgg tgcggataag gtgtctctta acactgcggc agttaacaat cccaagcttg
     8941 tttatgaagg agcaaaaagg tacggcagcc agtgcattgt ggtggcagtg gatgcaaagt
     9001 tcaagccggg tacagccgga tgggaagtct atatccgagg cggaaggacg gccacaggaa
     9061 tcgacgtttt ggagtggtgc aagaccgtta atgaattggg cgcaggggaa atactcctaa
     9121 ccagcataga tgcagacggc tctaagaaag gctatgatat agcattgaca tcaaaagtgg
     9181 ctgaaacagt aaatatacct gttatagctt cggggggtgc gggcagcagg gaggatttcc
     9241 tggaggtgct tacaaaaggt aaggctgacg cggcactggc ggcgtcgctg tttcactttg
     9301 gagaactgcg cattggggat ttgaagttgt atcttgcttc caagggggta gacgtaaaat
     9361 aagaagagtg cgatgtaggt aacgggtaac actaaacaca cgatatttca cggaatcgct
     9421 acgcgattac acggtaacta ttatctatta tatagataca taagaagatt ttgatacttg
     9481 catcattacc gataattatt tatgtatcgg aatatttatc ccgccgtgcg tttagcggtt
     9541 tttcgtgtct
//
LOCUS       DAOOAB010000073         9472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_9007_length_9472_cov_13.918233, whole genome shotgun sequence.
ACCESSION   DAOOAB010000073 DAOOAB010000000
VERSION     DAOOAB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9007_length_9472_cov_13.918233"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKU88_11600"
     CDS             complement(<1..545)
                     /locus_tag="PKU88_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX69954.1"
                     /translation="MKMKLLLVDDHQLFMEGLQYLLETYGVEVVGTAEDGREAFEKAR
                     VLKPDTILMDIKMPGLSGLDALKLIKAEMPDIKVVMLTTSEEDDDLFYAVKCGASGYL
                     LKNTNAKELVGILSDIEKGEATFSPELAAKLLKEFRSRDTGKTIQNEELTKRQLEVLE
                     LVSKGIIYKEVGEKLGLSERTV"
     gene            complement(520..2262)
                     /locus_tag="PKU88_11605"
     CDS             complement(520..2262)
                     /locus_tag="PKU88_11605"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPX69955.1"
                     /translation="MRIQYIPYIWPLITSAFISLSLGIFVILRRRDSKGAVSFILSML
                     VVTIWSLGNALEMASIDFSTKLFWANVQYFAYCYSPLTLLAMCMEFTGYDRWIRNRKV
                     LWLAVIPTIIVILVWTDHLHGLIRYNMHMNYSGSFPVIDKKYGPAFYIHALYSHMLNI
                     LAWILLIKAVFFRNTVYKKQATALLVGLSMIVIPNVLYITGLSPIERYDITPLFFGPA
                     GLIITWGIFRYRLFDVIPVARAMVIENMDAGVIVLDLQDRVLDINPAMRRIIGYPDVK
                     TPAETVEKVCRKIPELACTCKDRNITHSEFVIGINEGMKVYEAFFSPLADQKGAMIGR
                     LVVAYDITEKKQEQERYLKQQWKNAVTEERERLARDLHDNLGQVLGFINLQAQGIEQE
                     LINADIDIVSHKLDKLVRASQEAHNDLREYIRNARNAEALEKDFMTGLTRNIASFEEQ
                     SGLNVKVNIPLGFTGDELRPSIRINILNIVKEVINNIRKHSEANNVKFEFAIEQRQLC
                     VTVEDDGKGFDVENCLNNNNTTRFGLNIIRERASEIGAQIGIQSEPGRGCRISLIIPL
                     NKEELQNENEITAG"
     gene            complement(2275..3063)
                     /locus_tag="PKU88_11610"
     CDS             complement(2275..3063)
                     /locus_tag="PKU88_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HPX69956.1"
                     /translation="MENSKDSLNRKVASALKEAERTCIPIEPISKTYPELKIKDAYEV
                     QRINVNELLASGNKITGKKIGLTSLAMQQFIGVDQPDYGQLFDFMEVKNGILERSRYL
                     NPKVEGEIAFVLKKDLQGPNVTAKDVMEATDYVLASIEVVDSRIKDWKIGIVDTVADN
                     ASSAMYVIGNKKVDPNSINLKAVSMGLYKNGEKVLSGVGADVLGDPALSVAWLINKLW
                     EYGVTLKKGEVVLSGSLTSALAAEAGDDFKVVFSELGEVSLRFV"
     gene            complement(3078..3851)
                     /locus_tag="PKU88_11615"
     CDS             complement(3078..3851)
                     /locus_tag="PKU88_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPX69957.1"
                     /translation="MKNEVKSKMESGLPVIGTFFELGDAAAVECIGLSGMDFLIIDTE
                     HGPFDVESAMDFIRAAELRGIEPFVRIKDHTRSSVLKMLDVGAKGLIIPNIETVEQVK
                     KLVEYGKYYPVGRRGFAPTRCGGYGFVEHAAGNLEEYFSLANNETMIIPQCETKGCLD
                     NIEEIVSIEGVDGIFVGPYDLSIALGMPAQFSNPVFTESVDRILNACKAAGKSIFIFS
                     GSVEAARKYIKDGFNGIAYSLDTNILVSALKDCVRQIKG"
     gene            complement(3957..5006)
                     /locus_tag="PKU88_11620"
     CDS             complement(3957..5006)
                     /locus_tag="PKU88_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HPX69958.1"
                     /translation="MFIAITIIIGIIGGLTAVRLKMPAGAIIGSLISVALFSIFTGKA
                     EFPQSFKLVTQIGTGAYIGARICNKDVAEIKSIIKPAIILTVSMCLFGIFAGTLLSII
                     SDIDLTTALFATAPAGIADMTLISVDFGADSSKVAAVQLVRLVSVIAIMPNLIKYYLK
                     KSNKAQIEDKPPEKYEESCADYNEIFKRTLITMTVGFVFGFIGHVTGFPAGSISFSMI
                     ACAVYNIRTSRAYMPINLRKGIQFLGGALIGARITMEQVMGMRGMLPALIIIIAGYIL
                     LTFVSGFLLIKFSTLDVTTAMYSSTAGGLTDMAIIAGEMGADAPKVAVLQFARVVSII
                     AFYPVVIKLLLHILT"
     gene            complement(5210..>5461)
                     /locus_tag="PKU88_11625"
     CDS             complement(5210..>5461)
                     /locus_tag="PKU88_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPX69959.1"
                     /translation="GELSIEMEKVLNSMPNSPGIKADKILEINTDHEVFKSLKEAFEK
                     DKEKLNLYTNLLYNQALLIEGLPIGDPVEFTNNICKIMV"
     assembly_gap    5462..5561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5562..6745)
                     /gene="htpG"
                     /locus_tag="PKU88_11630"
     CDS             complement(<5562..6745)
                     /gene="htpG"
                     /locus_tag="PKU88_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPX69960.1"
                     /translation="MAKKQFKTESKKLLDLMINSIYTHREIFLRELISNASDAIDKIY
                     YKALTDESISFDKDSYYIKVVPDKENRTLRISDTGIGMTKEELDDNLGVIAKSGSLSF
                     KKEIELKDGHDIIGQFGVGFYSAFMVSDTVTVISKAYGSDDAYKWQSSGADGYTIELC
                     EKDTVGTDIILKIKENTEDEKYDEYLEEYRLKAIIKKYSDFIRYPIKMDMVSKRLKEG
                     SDKEYEGYKEEQIINSMVPIWKRNKNELTKEDYDNFYSEKHYGFDKPLKHIHISVDGA
                     VSYNAILFIPEKMPFDFYTKEYEKGLELYSKGVMIMNKCPDLLPDYFSFVKGLADSED
                     LSLNISREILQHDRQLKLIAKNIKNKIKSELENLLKTERDKYEEFYKAFGRQLKYGIY
                     SE"
     gene            complement(6983..8002)
                     /locus_tag="PKU88_11635"
     CDS             complement(6983..8002)
                     /locus_tag="PKU88_11635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPX69961.1"
                     /translation="MKIITFTVPCYNSEAYMKNCIESLLLGGEDVEIIIVNDGSTDGT
                     GEIADDYASRYPGIVKAIHQENGGHGEGVNTGLRNAAGLYYKVVDSDDWLDADGMLKV
                     LSKLKELHEKGTALDMMVCNYVYEHSSDNSSHVVNYKYVLPQNRVFGWDEIGNFKPTQ
                     YLLMHSVIYRTELLRQCGLVLPKHTFYVDNLFVYNPLPYVRTIYYMNIDLYRYFIGRE
                     DQSVNEKNMIKRIDQQIRVNKLMIDAYNSYDYLPSRRLANYMRSYLSMMMTISSVFLQ
                     ISATKEDLQKKAELWSYLKKSNEMLYYRLRYRFLGTLSNPYGKVGRKITIQLYRLMRR
                     IYKFN"
     gene            complement(8023..9177)
                     /gene="glf"
                     /locus_tag="PKU88_11640"
     CDS             complement(8023..9177)
                     /gene="glf"
                     /locus_tag="PKU88_11640"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HPX69962.1"
                     /translation="MKNEFDCLVIGCGFAGAVIAREHAERGGKKVLIIDKRSHIGGNA
                     YDSYNDDGILIHNYGPHIFHTQSSRVFEYLSRFTEWRDYSHEVLADIHGKLVPVPFNL
                     NSLYLVFEQSKAERLEQKLISVYGTEKRVTISELRGQTDSELLELADFIYKNVFMYYT
                     QKQWGIAPENIDPSVTARVPILISYDNRYFQDEYQGIPLNGYTSLFERLIDHPNISLQ
                     LNTDSASVLKLVENRIYFCGSPFYGRVIYTGAIDELFDCRFGRLPYRTLDFLFETHEK
                     TWYQSKGTVNYTVDRPYTRITEFKHLTGQIIPDKTTIMKEYPREYTGAGKEIPYYAII
                     NPENEALYEKYKQITEAIDDFYLLGRLAEYKYYNMDVIAERALSLADSII"
     gene            complement(9245..>9472)
                     /locus_tag="PKU88_11645"
     CDS             complement(9245..>9472)
                     /locus_tag="PKU88_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX69963.1"
                     /translation="LRKGNIHGAALDVFEKEPLPEESPLWEMNNVIITPHNSWISEMR
                     TERRYNIVYENLRRYINGEALLNIVDIDKGY"
BASE COUNT         2578 a         2245 c         1596 g         2953 t
ORIGIN      
        1 actgtccgtt cagaaagtcc gagcttttcc cctacctcct tgtagataat gccctttgac
       61 accagctcca gcacttccag ctgccgcttt gtgagttcct cattctgtat ggtttttcct
      121 gtatcccggc ttctgaattc cttcaagagc ttggcagcga gctccggtga aaaggtcgct
      181 tccccctttt ctatgtccga caatatacct acaagttctt tcgcattggt attcttaagc
      241 agatatcccg aggcaccgca cttgacggca taaaaaaggt catcatcctc ttccgaggta
      301 gtcagcatca ctacctttat gtccggcatt tcggccttta tcagcttaag ggcatccaac
      361 ccgctcaaac ccggcatctt tatatccatt aatatagtgt ccggtttcaa tactctggct
      421 ttttcaaaag cttctctccc gtcttccgct gtaccgacta cctctacccc ataagtttca
      481 aggaggtatt gaagtccttc cataaaaagc tggtggtcat caaccagcag taatttcatt
      541 ttcattttgc agttcctcct tattcaaagg aataattaaa gaaatacggc agcctctacc
      601 cggttctgat tgaataccaa tctgcgcacc tatttctgat gcacgctccc taatgatgtt
      661 taacccgaac ctggttgtat tattgttatt aagacaattc tcaacatcaa atccttttcc
      721 gtcatcttcc acagttacac ataactgccg ctgttcaatt gcaaattcga atttcacatt
      781 attggcttca gaatgcttcc tgatattatt aatgacttct ttaacaatat tcaatatatt
      841 gatgcgtatg ctcggtctta gttcatctcc tgtaaatcca aggggaatat ttacttttac
      901 atttaatcct gactgctcct caaagcttgc aatattcctt gtcagcccgg tcataaaatc
      961 cttttccaaa gcttctgcat tcctggcatt gcggatatat tcccttaaat cattatgggc
     1021 ttcctgggaa gcacgaacta gcttgtccag cttatgagat acaatgtcaa tatctgcatt
     1081 tataagctcc tgttctattc cctgggcttg aagattaata aatcccaata cctgtccaag
     1141 gttgtcatgg agatctctcg ccagccgttc cctttcttct gtaacagcgt ttttccactg
     1201 ctgcttcagg tatctctcct gctcctgctt tttttcagtt atatcgtagg caacaacaag
     1261 ccttcctatc attgctcctt tttgatctgc aagaggggaa aagaatgcct cgtaaacctt
     1321 cattccttca ttaatgccta ttacaaattc actgtgagtt atgttcctgt ccttacaggt
     1381 acatgccagt tccggaattt tacggcacac tttctctact gtctctgcgg gagtcttaac
     1441 atccggatag ccaattattc ttctcatggc cggattgata tccagcactc gatcctgcaa
     1501 atccagcact ataacccccg catccatatt ctcaatcacc atggctcgtg ccacgggtat
     1561 tacatcaaac aatctatacc ggaatattcc ccaggtaata atcaatccgg caggcccaaa
     1621 aaaaagcggt gtaatgtcat atctctctat cgggcttagt cctgtgatat atagaacatt
     1681 tggtattaca atcatactca gccccaccaa tagtgctgtt gcctgcttct tgtaaactgt
     1741 gtttctgaaa aagacggcct ttatcaaaag aatccatgcc agaatattca gcatgtgtga
     1801 atataaggca tggatataga aagcagggcc atatttttta tcaataacag gaaatgaacc
     1861 gctgtaattc atatgcatat tatatcttat caagccatgc agatgatccg tccatacaag
     1921 aattacaata atagtcggaa taacagcaag ccacagtact ttccggtttc ggatccatct
     1981 gtcatatccc gtaaactcca tacacattgc aagcagggtc agcggagaat aacagtatgc
     2041 aaaatactgt acatttgccc aaaaaagctt tgtagaaaaa tctatgcttg ccatctcaag
     2101 ggcattgccc agggaccata tggtcacaac aagcatactc aatatgaagc ttacagcccc
     2161 ttttgaatcc cttcgtctca gtattacaaa tattcccagg gataatgaaa taaatgcaga
     2221 tgtgataagg ggccatatat atggaatata ttgtatccgc atttatttca actcctatac
     2281 aaacctcagt gacacttctc ccagttcgga aaaaactacc ttaaaatcat cccctgcttc
     2341 tgcagccagt gccgaagtca gagaaccgga taaaaccact tctccttttt tcagtgttac
     2401 tccatattcc cacagcttat taataagcca tgccacagaa agggcaggat ctccaagtac
     2461 atcagcgcca actccggata ataccttttc cccattctta tataaaccca ttgatacggc
     2521 tttcaaatta atgctattgg gatcaacctt tttattcccg attacataca ttgcagaaga
     2581 tgcattatcg gcaacagtat ccacaattcc tattttccag tctttgattc tgctgtcaac
     2641 cacctcaatt gaggctaata catagtctgt tgcttccata acatcttttg ccgttacatt
     2701 aggcccctgc aaatcctttt tcaatacaaa ggctatttct ccctcaacct tcggattaag
     2761 atatctgctt ctttccagaa taccattctt tacttccata aaatcaaaca gttgaccgta
     2821 atccggctga tccactccaa tgaattgctg cattgcaagg gaagtgagcc ctattttctt
     2881 accggttatc ttattccctg atgcaagaag ttcattaaca ttaatacgct gtacttcata
     2941 agcgtccttg attttcagtt cgggatatgt ctttgatatg ggctcaatcg gtatacatgt
     3001 cctttcagct tccttcaaag ctgatgcgac cttccgattc aagctgtctt tgctattctc
     3061 catctttatc attcccttca gcctttgatc tgcctgacac agtcctttaa ggcactgact
     3121 aaaatattgg tatcaagact atatgctatt ccattaaaac catccttgat atattttctt
     3181 gcagcttcca ccgatccgct gaatataaat atggattttc cggcagcctt gcaggcattc
     3241 agtatcctat caaccgattc ggtaaaaacg ggattgctga actgtgcagg catccccaag
     3301 gctatggata aatcatatgg gccaacgaat ataccatcca ctccttcaat ggatacaatc
     3361 tcctcaatgt tatccaggca tccctttgtc tcacactggg gtataatcat cgtctcatta
     3421 ttggctaaac taaaatactc ttctaaattt cctgcagcat gttcaacaaa tccgtatcct
     3481 ccgcaccttg tgggagcaaa gccccggcgg ccgacgggat aatacttgcc atactccacc
     3541 aattttttta cctgttccac tgtttctatg ttgggaataa tcagaccctt agctcctaca
     3601 tcaagcatct tcagtacaga agaacgtgta tgatccttta tccgcacaaa aggttcaatc
     3661 cccctaagtt ccgctgcgcg tataaaatcc atggcgcttt ccacatcaaa gggaccgtgc
     3721 tctgtgtcga ttataagaaa atccattccg gaaaggccga tgcactctac ggcagcagca
     3781 tcacccaatt caaagaatgt accgattaca ggcaatccac tttccatttt gctttttact
     3841 tcgtttttca ttgctaacct cctgcatatg ctgaatatcg ttttctatac agtccaatta
     3901 taacataaaa gaaaatcttt attcctgcag caccatgaaa atttccctgt acaatattaa
     3961 gtcaaaatat gtaaaagcag cttgattact acaggataaa aggcaataat actgacaact
     4021 cttgcgaatt gcagcacagc aacttttgga gcatctgccc ccatttcgcc agcaattata
     4081 gccatatcgg taagccctcc tgctgttgag gaatacatgg cagtggtcac atccaaagta
     4141 ctgaacttta taagtaaaaa tcccgataca aaagtaagca gtatatatcc tgcgataatt
     4201 ataatcaaag ccggcagcat ccctctcata cccataacct gttccattgt tatcctggca
     4261 cctattagag ctcctcccag aaattgaatg ccctttctta aatttatagg catatatgct
     4321 cttgaagttc ttatattata cacagcgcaa gctatcatgg aaaagctgat agacccggcc
     4381 gggaaacccg tgacatgccc gataaatcca aaaacaaacc ctacggtcat cgttattaat
     4441 gtcctcttga atatttcatt ataatccgca caggactctt catatttttc gggcggtttg
     4501 tcttcaatct gagctttatt tgattttttt aagtagtatt ttattagatt cggcataata
     4561 gcaattaccg aaacaagcct cacaagctgc actgctgcaa cctttgaaga atcagcacca
     4621 aaatccactg aaataagcgt catatctgct atacctgccg gagctgttgc aaaaagtgct
     4681 gtcgtcaaat ctatgtctga gatgatactt agcagggtac ccgcaaatat gccgaaaagg
     4741 cacatagaaa cagttaatat tattgcaggc ttgataatac tctttatctc agcaacatct
     4801 ttattgcata ttcgtgcacc aatatatgct ccggttccta tttgagtcac cagcttaaag
     4861 ctttgaggaa attcagcttt tccggtgaaa atactgaaca gtgctacaga aattagcgaa
     4921 ccgattattg ctcctgccgg catcttcagt ctgacagctg tcaaaccgcc gataatgcca
     4981 attataattg ttatagcgat gaacataaaa aagcctcctt gtaggagtcg tcttgaccgc
     5041 atatgcttat agtagcatat tttcaatttt cagtcatatt ttgtttttcc attgtgcgct
     5101 tattatatat gaaaaagaac gttctccctt aatataataa aaattcctgc ctgtttcgga
     5161 cttttccgca gcatcaggca ggaaccaatc attgttaacc ctattgcacc taaaccatta
     5221 ttttacaaat attattcgtg aattctaccg gatcgcctat gggcaaacct tctatcagaa
     5281 gggcttgatt atataataga ttcgtgtaca ggttcagctt ctctttatcc ttttcaaagg
     5341 cttccttaag ggatttgaag acttcatgat cggtatttat ctccagaatc ttatcagctt
     5401 tgatacccgg actgttcggc attgaattaa gcaccttctc catttcaata gaaagctctc
     5461 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naattcgctg tatattccgt
     5581 atttcagctg ccttccgaag gctttgtaga attcctcgta cttatccctc tcggttttca
     5641 acagattctc cagttcgctc ttgattttgt tttttatatt cttggcaata agcttcagct
     5701 gcctgtcatg ctggagaatt tccctggata tgttaaggga caaatcctcc gagtccgcaa
     5761 ggcctttaac aaagctgaaa taatccggaa gcaggtcagg gcacttgttc attatcataa
     5821 caccctttga gtaaagctcc aggccttttt catattcctt tgtatagaaa tcaaagggca
     5881 tcttttccgg aatgaacagt attgcattat agctcacagc gccgtcaaca cttatatgaa
     5941 tatgctttaa aggtttgtca aagccgtagt gcttttccga atagaagtta tcataatctt
     6001 ccttggtcag ttcgttcttg ttccgtttcc atatgggcac catactgttt ataatctgct
     6061 cttccttata gccctcgtat tccttgtcgc tgccttcctt gagacgtttg ctgaccatat
     6121 ccatttttat cggatacctg ataaagtcgg aatatttctt tataatagcc ttaagcctgt
     6181 actcttcaag atactcgtca tatttctcat cttcggtatt ctcttttatc tttagtatga
     6241 tatccgtacc gacagtatct ttctcacaaa gttcaatcgt ataaccgtct gccccgctgg
     6301 attgccactt gtacgcatca tcacttccgt aggccttgct tattaccgta acagtatcag
     6361 ataccataaa tgccgaatag aatcccactc cgaactggcc tattatgtca tgcccgtcct
     6421 ttagttcaat ttccttctta aatgaaagag aaccgctttt tgctattacc ccaagattat
     6481 catccagttc ttcttttgtc attccgattc ctgtatccga aatcctgagg gttctgtttt
     6541 ccttgtcagg tacaaccttt atataataac tgtctttatc aaagcttata gattcgtccg
     6601 ttaatgcttt gtaataaatc ttgtcaatgg catcacttgc attggaaata agctccctga
     6661 gaaaaatctc cctgtgagtg taaattgaat tgatcatgag atccaatagc ttcttggatt
     6721 ctgttttgaa ttgtttttta gccataaaaa ttctcctccc aaagaaaatg tcatatgtat
     6781 tgttagcact ctgcctattt gagtgctaac cctctatcca ttatttatca caattcaatt
     6841 tctatgtcaa ggcttattat gtaaaagaag aagaatacga cgggggacac ggggaggaca
     6901 cggagacgtt tctcttgtcc cgctttcagc gggacaagag aaacgtcccc gtgtcctccc
     6961 cgtgcctttt cgccgcattc ttctaattga atttgtatat cctgcgcatg agccggtata
     7021 attgaattgt aatctttctt ccaaccttgc cgtaaggatt ggataatgtt cctaagaagc
     7081 ggtatctcag cctgtaatag agcatttcat tgctcttttt aagatatgac cacaattccg
     7141 ctttcttctg caaatcctct tttgtggcgg agatctgaag aaacaccgat gaaattgtca
     7201 tcatcatgga aagataactt ctcatataat ttgcaagccg tctgctgggc agataatcat
     7261 aggaattata ggcatctatc ataagcttgt ttactcgaat ttgctgatca attcgcttta
     7321 tcatgttttt ttcatttacc gattgatctt ctctcccgat aaaatagcga tacagatcaa
     7381 tattcatata ataaatggtt cttacataag gaagcgggtt gtacacgaat aggttgtcca
     7441 cataaaaagt atgcttgggc agtaccaggc cgcattgccg aagaagctcg gtacgataaa
     7501 taaccgaatg catgagaagg tattgagtcg gtttgaagtt acctatctca tcccagccga
     7561 aaacacggtt ctggggcaat acatacttgt aattgacaac atgactgcta ttgtctgatg
     7621 aatgctcata aacataatta cataccatca tatcgagagc agtacctttt tcatgcagct
     7681 ccttaagctt ggacagcact ttcagcatac cgtcggcatc cagccaatca tccgaatcca
     7741 caaccttgta atacaaaccg gcggcatttc tcaaaccggt attgacaccc tcgccatgtc
     7801 ctccgttttc ctgatgtatc gctttaacaa tgccgggata ccgggaagca taatcatccg
     7861 caatttcacc cgttccatcc gttgaaccgt catttacaat gattatttca acatcttctc
     7921 ctccgagaag caaagattct atgcaattct tcatatatgc ctcggaatta tagcaaggaa
     7981 ctgtaaaagt tattatcttc atatcccact ctctttcccg cattatatga tgctgtcggc
     8041 cagcgacaat gcccgctctg ctattacatc catgttgtag tatttgtatt cagccagcct
     8101 gccaagcaga taaaaatcat caatagcctc agtaatctgc ttatactttt catataatgc
     8161 ttcattctcc ggatttatta tagcatagta gggtatttct tttccagcac cagtatattc
     8221 cctgggatat tctttcatga tagtggtttt atccggtatt atctggcctg tcagatgctt
     8281 gaactccgtt atgcgggtat aaggccggtc aactgtataa tttacagttc ccttagactg
     8341 ataccaggtc ttttcatgag tttcaaacaa aaaatccaga gttctgtatg gcagccggcc
     8401 gaagcggcaa tcaaaaagct cgtctatagc accggtatat attactctgc cgtaaaacgg
     8461 gctgccgcaa aaatatatcc tgttttcaac cagcttaagc acggaagctg aatcagtgtt
     8521 aagctgcagg cttatgttcg gatgatcaat cagcctttca aaaagggaag tgtagccgtt
     8581 taatggaatg ccctgatatt catcctgaaa ataccgattg tcataggaaa taagaatggg
     8641 aacccttgcc gtcactgaag gatctatgtt ttccggagca ataccccatt gtttttgtgt
     8701 gtaatacatg aaaacatttt tgtatataaa gtctgcaagt tcaagcagtt cgctgtcggt
     8761 ctgccctctc agttcagata ttgtcacccg cttttccgtt ccataaaccg aaatgagctt
     8821 ttgctcaagc ctttcagctt tgctttgttc aaaaaccaga taaagagaat tgagattgaa
     8881 gggaaccgga accagttttc cgtgaatatc ggccagaacc tcgtgagagt aatccctcca
     8941 ctctgtgaaa cgggaaaggt attcaaatac acggctgctt tgagtatgga aaatatgagg
     9001 gccgtaatta tgaattagta ttccgtcatc gttgtatgaa tcataagcgt tgccgccaat
     9061 atggctgcgc ttatcaataa tcaaaacctt tttgcctccc ctttccgcgt gttccctggc
     9121 tatcaccgct cctgcaaagc cacagcctat gacaaggcaa tcaaattcat ttttcatttg
     9181 ccggaattac ctccatttat ttatatactt ctatcaatga ctcatataaa tacacatatt
     9241 ttcatcagta tcctttatca atgtccacta tattaagaag cgcttcccca tttatatacc
     9301 ttctgagatt ttcataaact atattatatc ttctttcggt tctcatttcg gatatccagg
     9361 aattatgggg tgtaataata acattgttca tctcccataa agggctttcc tcaggcaaag
     9421 gctccttctc aaacacatcc agcgccgctc cgtgaatatt tcccttccga ag
//
LOCUS       DAOOAB010000074         8881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_9699_length_8881_cov_19.716179, whole genome shotgun sequence.
ACCESSION   DAOOAB010000074 DAOOAB010000000
VERSION     DAOOAB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_8881_cov_19.716179"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..72)
                     /locus_tag="PKU88_11650"
     tRNA            complement(1..72)
                     /locus_tag="PKU88_11650"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ala,seq:cgc)
     gene            complement(89..1354)
                     /gene="glyA"
                     /locus_tag="PKU88_11655"
     CDS             complement(89..1354)
                     /gene="glyA"
                     /locus_tag="PKU88_11655"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPX69964.1"
                     /translation="MLKRTRITDPEIYEIVADELRRQEHNIEMIASESTAPLEVMELS
                     GCIFTNKTLEGYPGARFQAGSHEADRLELLGIERAKKLYGAEHVNFQVYSGSTANYGV
                     YAAVLKPGDKVLSMRLDQGGHLTHGSPANFLSKVYEYDFYGVNPETEMIDYDELEKKA
                     EEIKPRLIIAGGSSYPRLIDYERISKIAKAAGAYFMVDMAHISGLVAAKVIPSPVPHA
                     DFVTSSTTKTFCGPRSGFILCKKEYAKLIDKGVFPGSLGSMHLTTMAAKAWSMKYAAS
                     AEFRSIMKQILVNAKTLAEELSRLGFRIVSGTTDNHIVMVDLRSKGISGKLFQDALDE
                     VGITVNKNQIPFDPASPLVTSGVRIGVTSITQRGLKEVEVKEIAEIMNQVAAAPEDEA
                     NLEACRERAEALIARFPLYPAGSFEDYNV"
     gene            1558..2415
                     /locus_tag="PKU88_11660"
     CDS             1558..2415
                     /locus_tag="PKU88_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPX69965.1"
                     /translation="MRFEKLYADPIIEDPGFPPESMAVVINSGGKNLYGVLYTAAGMG
                     PHPSMILLHGFPGTERNLDMAHAFRRAGWNTLVFHYRGSWGSEGCFSFQNVLDDVKAA
                     IEFVKSEEPARKYRIDRDNIVLVGHSMGGFAALLTAADDMDIKAYAAIAPFDFGAIGE
                     NSEGDDKALRFLQGMFKECIVPLKGNTVEELINEAMANGNKWSFINNAEKLAKHKLLL
                     IAAKRDNLSIPELHYYPLVNKMLKYNTENFEYKMIDSDHSFQDKRILLTEIIEGWLEK
                     QVQQKSNIK"
     gene            2529..2816
                     /locus_tag="PKU88_11665"
     CDS             2529..2816
                     /locus_tag="PKU88_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69966.1"
                     /translation="MSFLKNLSEKVVSTAKVVGDKSQDMVEVSKLKLHISQIEGDIKK
                     LKFNMGELVYNAFASDTEFPAEEVTRIGEEIKAKYTEIEETKIKIQEVQAN"
     gene            complement(2900..3586)
                     /locus_tag="PKU88_11670"
     CDS             complement(2900..3586)
                     /locus_tag="PKU88_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPX69967.1"
                     /translation="MLKAFALGITTGTSATTFSPQVLINREQCAAMLFRAIKAIHPNG
                     DYSVAGIPDFPDQKDMANYAVEAAKYMSKLAIVKGDAKGYFMPKATTTAQEAAGYGMA
                     TREAAILMSVRTYGQMDTIKAAPAAAVPIASASVVGTWILGTLSGGQFNAVTGKYEGG
                     ASGLGQMYTFKEDGGKTWGARETLTDTSAYFTAGTDDSGKYLLLGEEGSVPPLADKEN
                     ALKYKLDTAF"
     gene            complement(3559..4107)
                     /locus_tag="PKU88_11675"
     CDS             complement(3559..4107)
                     /locus_tag="PKU88_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69968.1"
                     /translation="MTFINGKLYNNSERWSMSSLFQEDEELKAFYEGGWDYLKSHSVT
                     FRARFAESFDYGETTVLSPWSREFTLSANTKADYNKLINHAPTILSAEAKLKPDGEPY
                     FQVKLDKVPGEVQDLNSMASGSMRTEIWMRRAGDKDFKQIHYEWATWEYLEVSAKALH
                     LLLKTPLQTRKTLRCLRPLLSA"
     gene            complement(4356..4853)
                     /locus_tag="PKU88_11680"
     CDS             complement(4356..4853)
                     /locus_tag="PKU88_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69969.1"
                     /translation="MKRILACLLVCSCLLAVILPLGGCGIKKKAEQAISEKIVEKALG
                     DEVDIKGDTVTVKGEDGEEITVGGGKWPDTDLAKKIPEFKKGKIATSAFQDNNIMVIM
                     DEVKADDFEAYLKEIKKTYTQDVYESRTDEMIIFGGNDGNQITVALNFMVKDGTMGIS
                     VQKDE"
     gene            complement(5122..6726)
                     /locus_tag="PKU88_11685"
     CDS             complement(5122..6726)
                     /locus_tag="PKU88_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX69970.1"
                     /translation="MNRLSETTRKIQYHVRRERASGISMRYRLFGFLFVLVLTLLSFV
                     FIILLLTGTFTAGLREAEGFYSNEFKRISSAVEKEYGHVSASAVSLSSSLSQSMERFL
                     SEKGLSTADLPEAPEVLEELLDREFDRLLFSLEKTGASGAFIVLDATVNKQAEAAEFS
                     RAGLYLKNMEPNIISSAPPDVTILRGFPDIARDRGYSLHAQWDMEFMIDSPSHFFLPF
                     EKAKEKPRPLSRLYYWSGAVTLPKTSEDAMLCIVPLIDSSGNIFGVCGYEVSAMLFKL
                     KFIPDSSAYQRIFCMLAPIEDSVLRVGSALFSGNYDAFGVSGDKKPLIIKNRGRLLYS
                     YEQQNGLSLAGLHKEIRLYPEDSAFAEEHFAVGILMPQEDLGRRLAWMNIRLIVLCAL
                     FLLAGTAASVFIGNKYMNPIMKGLNAIGSQDIDSIARTNITEIDEIIGQIKARRTGKA
                     PLPDDIFTDFIGRVKTLTPTEKMIFRYYLEGKSNGEILELMYISANTLKTHNNHIYTK
                     LNVSSRNELSLYISLMEKSGRLSELN"
     gene            complement(6742..8094)
                     /locus_tag="PKU88_11690"
     CDS             complement(6742..8094)
                     /locus_tag="PKU88_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPX69971.1"
                     /translation="MMKVRALCLMLIFLILLCSVGCSTKTVLNPKSPVTLTMWHNYGG
                     QMKNLMDEMIDEFNRTVGTEKGIIISVTSISGSSALHDKLISAAFGDPGAPELPDLAV
                     VYPKTALKLVEKDLLVDLSGRFTEEELSAYVPRFVEEGRLKGGLFVFPIAKSTEALFV
                     NKSIFDRFAADTGSTYDELSTFEGIMRTADKYHEWSEGKAFYMPDSVFNMAIIGMNQL
                     GEDFVQNNGLNTAGRVYKKIWDTYFLSAVKGSTAIFDGYSTDLFKTGDIVCSTGSTAG
                     VLFYPSTVTYADNTKENAEYAILPYPVFEGGRKVALQRGSGMCVVKSDEQKEIAAAIF
                     LKWFTAPEQNLHFTASSGYLPVTEKAFGDSMSKQMEGISEENIKKLLGTIMVMQEDYD
                     FFIPPLTDNYDELQKGYEARLKKAAKAAKAEFDALDEAADKSAALETLSRSMYEDFIK
                     "
     gene            8610..>8881
                     /gene="gatC"
                     /locus_tag="PKU88_11695"
     CDS             8610..>8881
                     /gene="gatC"
                     /locus_tag="PKU88_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPX69972.1"
                     /translation="MKISVEDVRYIAKLAKLSITFEEAQKMVGEFEGILTYFESIDRL
                     DSGDALPDPPEREMATVLRKDEISVFEDKEKLFLNAKSMRDSYIRVP"
BASE COUNT         2378 a         2151 c         1850 g         2502 t
ORIGIN      
        1 ggacgatagg gggttcgaac ccccgacctc tgcgttgcga acgcagcgct ctcccaactg
       61 agctaatcgc cctccctccc aataatcatt atacattata atcttcaaag ctgccagcag
      121 gatataacgg aaatcttgca atcaatgctt cagccctttc tctgcaagcc tccagatttg
      181 cttcgtcttc cggtgcggct gccacttggt tcattatttc tgcaatttct ttaacttcaa
      241 cttctttcaa tccccgctgg gttattgacg taactccgat tcttacaccg ctggtaacaa
      301 gcggcgacgc aggatcaaag ggtatctggt tcttattgac ggttattcct acttcatcaa
      361 gagcatcctg gaacagcttt ccgcttattc ctttggatct caaatctacc attacaatat
      421 gattgtccgt tgtgccgctg acaattcgga acccaagacg ggacagctct tctgccagag
      481 tcttcgcatt cacaagtatc tgcttcataa tactgcggaa ttctgcagac gccgcatact
      541 tcatcgacca tgcttttgcc gccattgttg ttaaatgcat tgatcccagc gaccccggga
      601 agaccccttt gtcaatcagc tttgcatatt ccttcttgca gagaataaat ccgcttctgg
      661 gtccgcaaaa agtctttgta gtcgatgatg ttacaaaatc ggcgtgaggc accggactgg
      721 gtatgacttt tgccgccacc aacccgctta tgtgtgccat gtcaaccata aaataagccc
      781 ccgcggcttt tgcaattttg cttattcttt cgtagtcaat cagtctcggg tatgaactgc
      841 ctcctgctat aatcaatctg ggttttattt cttccgcctt tttttccagt tcatcataat
      901 ctatcatttc tgtttcggga tttacaccgt aaaaatcata ttcatacacc tttgaaagaa
      961 aattcgcagg acttccatgg gtcagatgcc ccccctggtc aagcctcatg cttaggacct
     1021 tgtctccggg cttgagaacc gccgcataga ctccataatt tgccgtggag ccggaataaa
     1081 cctgaaaatt cacatgctcc gccccgtata acttttttgc ccgctcaatc cccagaagct
     1141 ccaatctgtc cgcctcatgg gagcctgcct gaaagcgtgc tcccgggtag ccctccaggg
     1201 ttttattcgt aaatatgcat cctgacagct ccataacctc caaaggtgcc gtactttcag
     1261 aggcgatcat ttcgatatta tgctcctggc gtcttaattc atcagcaact atttcataga
     1321 tctcagggtc tgtaattcgg gtacgcttta acatttccat ccctctttcc aataatgttc
     1381 gcattcgctt actgctgatt ttaaagatta cctttcacgc tttactcaat aattatctac
     1441 aatatacaac aaatgaccgt atttgaaaag gctcaatgct ttagaaaata ttcccctctg
     1501 tgctataatt caagtatacg gaacctgatg accttgatga aataaggagg gtactttatg
     1561 cggtttgaaa aactatatgc ggatcctatt attgaagacc cgggatttcc tcctgaaagt
     1621 atggctgttg ttattaacag cggcggcaag aatctttatg gtgttttgta cacagcagcg
     1681 ggcatgggcc ctcatccttc catgattctg cttcacggct tcccgggtac cgagaggaat
     1741 ctcgatatgg cccatgcttt caggcgggca ggctggaaca ccctggtgtt tcattacagg
     1801 ggttcctggg gcagcgaagg atgcttctcc tttcaaaacg tgctggatga tgtaaaagca
     1861 gcgatagagt tcgtaaaatc agaggaacct gcccgaaaat accggattga cagagataat
     1921 atagtccttg tcggacacag catgggaggc tttgccgcac tgctgactgc cgctgatgat
     1981 atggatatca aggcatatgc tgcaatagcc ccatttgact tcggcgctat aggggaaaac
     2041 tccgaaggag atgataaagc tctgagattt ttgcagggaa tgttcaagga gtgtattgtc
     2101 ccccttaagg gtaatacagt tgaggaactg attaatgagg cgatggcaaa cggtaataaa
     2161 tggagcttca tcaacaacgc ggaaaagctg gcaaaacata agctcttatt aatagctgcc
     2221 aaaagggaca acctttccat tccggagctt cactactatc ccttggtaaa taaaatgctc
     2281 aagtataata ccgaaaactt tgaatataaa atgattgatt ccgatcattc ctttcaggat
     2341 aagagaatcc tgttaactga aataattgaa ggctggctgg aaaaacaagt tcagcaaaag
     2401 agtaacataa aataaatttt aggcatatca tgataaatat atgaataatt attgttaatt
     2461 agcaggatat tcctgttaaa cagcgaatta atcagtaaaa taaactccaa aaaaaaggag
     2521 gatggaatat gagtttttta aaaaatcttt cggaaaaagt cgttagtacg gcaaaagtag
     2581 taggtgacaa atcacaggac atggtggagg taagcaagct gaagcttcat atttcacaga
     2641 ttgagggaga tattaagaaa ctgaagttca atatgggcga gctggtttac aatgcatttg
     2701 cctcagacac tgaattcccg gctgaagaag taacccgcat cggagaagag ataaaagcaa
     2761 agtatacgga aatagaagag accaagataa agattcagga agtgcaggct aattaagtta
     2821 taggacaatt gagacagggg ggacgtttct cttgtcccgc aagcgggaca agagaaacgt
     2881 ccccctgtct cagtgagtgc taaaatgctg tatccagctt atacttcaaa gcattttcct
     2941 tatcagccag gggaggaact gagccttcct ctccaagcag cagatacttc cctgagtcgt
     3001 ccgttcccgc ggtgaaatat gcggaggtgt cagtcagcgt ttctcttgct ccccaggtct
     3061 ttcctccgtc ctccttaaat gtgtacattt gtccaaggcc tgatgcaccg ccttcgtact
     3121 ttcctgtaac tgcgttgaat tgtccccctg acagagtgcc gagaatccat gtcccgacga
     3181 cagaagctga agcaatcgga accgcggctg ccggggcagc ctttatggta tccatctgcc
     3241 cataagtcct cacgctcatc agtattgcag cctccctggt ggccatgccg tagccggctg
     3301 cttcctgtgc agttgttgtc gccttgggca taaaatatcc cttggcatca cctttgacta
     3361 tagccagctt tgacatgtat tttgccgcct caaccgcata gttggccata tccttctgat
     3421 cgggaaaatc cgggataccc gctacgctgt aatccccgtt ggggtgaatg gccttaatgg
     3481 cacggaacag catggcagca cactgttccc ggttaatcaa aacctggggt gaaaaggtag
     3541 tagcgctggt gcctgttgtt atgccgagag caaaggcctt aagcacctga gggtttttcg
     3601 tgtctgtaaa ggggttttga gaagcaggtg cagggctttt gccgatactt caaggtactc
     3661 ccaagttgcc cattcatagt gaatctgctt gaaatccttg tccccggctc ttctcatcca
     3721 tatctctgta cgcatggagc cggaggccat tgaattcaga tcctggactt cccccggaac
     3781 cttatccagc tttacttgga aatacggctc cccgtccggt ttgagtttcg cttctgccga
     3841 caagatagtg ggggcatgat ttatgagctt attgtaatca gcctttgtat tggcagaaag
     3901 agtgaattcc cttgaccagg gcgacaatac ggttgtctct ccataatcaa aggattctgc
     3961 aaagcgcgcc ctgaaggtga cggaatggct tttcaggtag tcccagccgc cctcgtagaa
     4021 agccttcagc tcctcatcct cctggaataa ggatgacatg ctccatcttt cactgttgtt
     4081 atacaatttg ccattgataa aggtcatgta catatcatcc agtccgtcag gaactgtttc
     4141 gcttgaatcc caggctgatg tatgatgcca ttagaactgc aagtaatcta cctaccactg
     4201 tacattcctc ccaaaatttt atttcatgcg atccaaggta ctcgcattgt cagcttattt
     4261 taaatatttc ctttgtcttc aggtaacttt atcatccctg ccgataaggc ggggaatata
     4321 tactctctgc ctgtaaataa aactgggatc aatccctact catctttttg aactgaaatt
     4381 cccattgttc cgtccttgac cataaaattg agggcaacgg taatctgatt cccgtcattt
     4441 ccgccgaaaa taatcatttc atcggttctt gattcataaa catcctgtgt atatgttttc
     4501 ttgatttcct tcagataagc ctcaaaatca tcagctttaa cctcatccat aataaccatg
     4561 atgttattgt cctggaaagc cgaggttgct atctttccct ttttaaactc cggaatcttc
     4621 tttgccaaat ccgtatccgg ccacttcccg cctccgactg taatttcttc tccgtcttcc
     4681 cctttgactg tcactgtatc acctttaatg tctacttcgt caccgagtgc tttctctact
     4741 attttctcag atattgcttg ttcggccttt ttctttatgc cgcagccgcc aagcggcagt
     4801 attactgcca acaagcagct gcataccagt aaacatgcca atattctttt catttatttc
     4861 tcctcctcta ataatttatt tcgccattac ggcaaataat tagatgttta ctcaagatgc
     4921 ttgttgaaat tttcaacatc atctccccct tcttttttca gcacattcaa tgcaaagatc
     4981 ctgatccggc atttattgaa gcttacttct attatgaaaa ataagtcttc aactgtatat
     5041 caccctaggg gtgattttcc aaaaatttca ataaaaaaac cccacctgat taaggtgagg
     5101 gaacagccaa gcctttaggg cctagttcaa ttcggaaagc ctgccgcttt tttccataag
     5161 gcttatatag agggaaagct cgttccgtga agatacattc agctttgtgt atatatgatt
     5221 attgtgtgtt ttcaaagtat tggcactaat atacatgagc tcaagtattt cgccgttgga
     5281 ttttccttca agataatacc ggaatatcat cttttcggta ggtgtcaggg ttttcaccct
     5341 cccgatgaaa tcggtgaata tatcgtcggg cagcggagcc tttcctgtac gcctggcttt
     5401 tatctgcccg ataatttcat ctatttcagt tatgttggtt ctggctatgc tgtcgatatc
     5461 ctgggagcct atggcattaa gccccttcat aatagggttc atgtatttat tgcctataaa
     5521 taccgaagca gcggtaccgg ccagtaaaaa caacgcacac aacactataa ggcggatatt
     5581 catccaagcc aggcgccggc ccagatcctc ctggggcatt aaaataccca cagcaaaatg
     5641 ttcttccgca aaagctgaat cctcagggta caaccggatt tctttatgca atcccgccag
     5701 gcttaatcca ttctgctgct cataggaata gagcaatcgg ccccggtttt ttataataag
     5761 gggcttttta tccccggaaa cgccaaaggc atcgtaattt ccggagaaaa gcgcactccc
     5821 cacccgcagg accgaatcct caatcggggc aagcatgcag aatatgcgtt gatacgcgga
     5881 gctgtcgggt atgaatttga gtttgaacag catggcgctc acttcgtagc cgcatactcc
     5941 gaaaatattt ccgcttgaat caataagagg cacaatacat agcattgcat cctcgctggt
     6001 ttttggcagt gtcacagccc cgctccagta atacagccga gaaagaggac gtggcttttc
     6061 cttcgccttc tcaaagggca gaaaaaaatg cgaaggactg tctatcataa attccatgtc
     6121 ccattgggca tggagagaat acccgcggtc ccttgcaata tccggaaagc cccttaaaat
     6181 agtaacatca ggcggagcgg aacttatgat attgggttcc atgtttttca gatagagccc
     6241 tgcccgggaa aactccgcag cttcggcttg cttgttgacg gtggcatcca aaacaataaa
     6301 agcgccgctt gcgccggtct tttccaaaga aaataacagc cggtcaaatt ccctgtccag
     6361 cagctcttcc aaaacctccg gagcttcagg caaatcagcg gtactgagtc ccttttccga
     6421 aaggaacctc tccatgcttt gggacaggga agaggacagg cttacggctg aagcggaaac
     6481 atggccgtat tccttttcca cagctgagga tattctctta aactcattgg aatagaaacc
     6541 ctcggcctcc ctgagccctg cggtaaaggt ccccgttaaa agcagtatta taaaaacaaa
     6601 gctcagcaat gtcaggacaa gcacaaacaa aaatccaaac agcctatagc gcattgatat
     6661 tcccgatgct ctttcccggc ggacatgata ttggattttg cgtgttgttt cggaaagtct
     6721 gttcacaatc tcacctgcgc tttatttgat gaaatcctca tacatacttc ttgataacgt
     6781 ttcaagagca gcgcttttat ctgctgcttc gtcaagagcg tcaaattccg ctttggcagc
     6841 tttggcggct tttttcagcc ttgcctcata tcctttctgc agctcgtcgt aattatccgt
     6901 aagaggcgga ataaaaaaat cgtaatcctc ctgcattacc ataattgtac cgagcagctt
     6961 tttgatgttt tcctcagata tgccttccat ttgcttgctc atggaatcgc cgaaggcctt
     7021 ttccgtaacc ggcagatagc cgcttgaggc tgtgaagtgc agattttgct ccggtgccgt
     7081 aaaccacttc aggaatattg ccgccgcaat ttccttttgt tcgtcggact tgacgacgca
     7141 catcccgctg cccctttgaa gggcgacctt cctaccccct tcaaataccg gatagggtaa
     7201 aatagcgtat tccgcatttt ccttggtatt gtccgcatag gtaacagtag aggggtaaaa
     7261 cagtactccg gcagtggagc ctgtggagca gacaatatcg ccggtcttga acagatcggt
     7321 gctgtacccg tcgaatatgg ccgtgcttcc tttgacagcg gaaaggaaat atgtatccca
     7381 tattttttta tatacccggc cggctgtatt cagcccgtta ttttgcacaa aatcctcccc
     7441 aagctggttc atgcctataa ttgccatatt aaacaccgag tcgggcatat aaaaagcttt
     7501 accctccgac cattcatggt atttatccgc cgtcctcatg attccctcaa aggtggaaag
     7561 ctcgtcatag gttgagccgg tatctgccgc gaagcggtca aagatgcttt tgtttacaaa
     7621 cagagcctcg gtggatttgg ctatgggaaa aacgaagagc cctcccttca gcctgccttc
     7681 ttccacaaaa cgcggcacat aagccgacag ctcctcctct gtaaaccggc cggataaatc
     7741 caccagcagg tccttttcga ccagcttcag cgcagtcttt gggtatacga cggcaagatc
     7801 aggaagctca ggggctcccg ggtcgccgaa ggctgccgat ataagcttgt catgcagcgc
     7861 acttgaaccg gagattgagg tcacacttat tataataccc ttttccgttc ccacggtacg
     7921 gttgaattca tctatcatct catccatgag gtttttcatc tgcccgccgt agttgtgcca
     7981 catggttaaa gtgacggggc ttttaggatt cagtacggtt tttgtactgc agcccacaga
     8041 gcataaaaga atcagaaaaa tcagcatcag acacaatgcc cgaaccttca tcataccacc
     8101 cctcttttgc ttttattata ccatttaatt ttataataca atcattaaaa taacataaaa
     8161 tgcaagttcc ggaacttgaa acataattaa aaggtggtct aattcttacg aatagaccac
     8221 ctcaagaacc aggctctaat tcaacggaat acctttgaat ctaccaaatg ttaactacct
     8281 caatcctttc aaaccaaaaa tcttaccaaa caaactctaa accaatttta ccaactacac
     8341 gaactacatt ccaaaactca aaccaaatgg gtcacagtac tttagggaaa ttgcacatta
     8401 gttgaattag agcctgtggg aaagatgtaa tcatctttct taatttaatt ataccacaaa
     8461 atacccataa tgcaatgcta attttcagaa tatttaaatt tttacttgtc catgagcttt
     8521 gacggctata tcggcaggaa acataatatc cgacggagaa gtataatgtc aatatattgg
     8581 aaaaactcta acttaatgga gtggttaaag tgaaaatatc ggttgaagat gtaagatata
     8641 ttgcaaagct tgccaagctt agtataacct tcgaagaagc acagaaaatg gtcggggaat
     8701 ttgaaggtat actgacatat ttcgaatcca ttgacaggct tgattcaggg gatgccctgc
     8761 cggatccgcc ggagcgggaa atggctacgg ttctgagaaa ggatgaaatc tctgtttttg
     8821 aagataagga aaaactcttt ctgaatgcca aatccatgag ggattcctat atcagggtgc
     8881 c
//
LOCUS       DAOOAB010000075         8053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_10834_length_8053_cov_16.179920, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000075 DAOOAB010000000
VERSION     DAOOAB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_8053_cov_16.179920"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1173
                     /locus_tag="PKU88_11700"
     CDS             97..1173
                     /locus_tag="PKU88_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPX69973.1"
                     /translation="MNYVIIMCGGSGSRFWPKSRKMYPKQFLNTVGERTMIQLTVDRI
                     SKFIPLKNIYMVTNKNYVNTIREQVPEILEKNLILEPMIKETAACIGYSAVKLLKKDP
                     EAVMIVLPSDHYIQDEDIFIETLKQGLRIADSENCLVTMGIKPTRPETAYGYIETGRK
                     IEGSWEIPTYKIKRFTEKPNREKAQEFIDKGTFLWNSGMFIWKASVLLKQYKKFLPDM
                     YQCLKRISDYIGSPLEAKIVEEEYNKIDGISIDYGILEKTWDVYVMESSFYWDDIGNW
                     TAMERYMPKDECGNTLKGECKVLDSHNCILYGDKRLIAVLGVDDLVIIETDDVILVCR
                     KDRDQDIKLLIKELQEDKENMKYI"
     gene            complement(1197..2522)
                     /locus_tag="PKU88_11705"
     CDS             complement(1197..2522)
                     /locus_tag="PKU88_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX69974.1"
                     /translation="MTEGNVYRHIIVFAIPIIIGNIFQQLYNIVDAIMVGRYIGEDAF
                     AAIGVASPIMGTALFIIVGMCIGASVLMAQLFGANDQKKLKKEISTAFIAGSIFSIIV
                     SITFIIVTNSILRWLNTPDNLMHDARVYLITIFSGLIFTMLYNLFDAIFRSMGNSRIA
                     LFFLIIASILHIILDLVFIIKMNMGVFGVGLATVISQAASALLCAIYGLNTYPILRFK
                     KEEIMIDLKLLKKTAEFGWIAALQQSCVYIGKMLVQSAVNPLGISAIAAFSAVSRIDA
                     FFVTPGDGVASALTTFIAQNTGAGKKDRIKSGLKTGFSLLSVYGFSISLLVYFGAYPL
                     MGIFVENEGQSIISLGAQYLQVMSLFYILSGFGYAFQGFFRGIGKIGITFFATFFQII
                     IRIILSYSLSPVFGLAGVALAVGIGWLSLPIYHGIMYYRYIKKSRLLIE"
     assembly_gap    2677..2776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2777..3570)
                     /locus_tag="PKU88_11710"
     CDS             complement(<2777..3570)
                     /locus_tag="PKU88_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPX69975.1"
                     /translation="MNICMDARGAKLYAGTGIGTYTARLLENIIKVDTRNNYRFFWPN
                     DGYDFLLGRGNISLTLFGEKNKKFWDEVFLPAQVKMNSCDVFHLPQNGLGLPRPKTCS
                     YVATVHDLIPLVMPDTCGKSYLDKFLQEMPYILEKCDRVITVSNYSKQDIIKYFNLPE
                     EKIKVIYLAADSRFRLIDKEKAWDFLKKEYSYSNDFILYLGGFSPRKNVDGLLEAYRR
                     ICRELPGYYDLVLLGASKDSHYELKKKTEGMGIRDRVVFAGYVPYE"
     gene            3691..4677
                     /locus_tag="PKU88_11715"
     CDS             3691..4677
                     /locus_tag="PKU88_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HPX69976.1"
                     /translation="MDLKAVEETYGIKIIDIQSIRNILRAETDCGVKCIKRAHMSPGF
                     FLFIYSAVNHLKEKGYEGVIPYNRAIDGSICIPDGKHVYYVVDWIEARECRFRKEEEL
                     KMAIKAAAELHRASVGYVPPEGAKPRVFYNKWVEKCEKKSKELLEFGKAIEDKEHRDD
                     FDEIFALHLPYYLQQSLDTANMIMESPYWEISEKSEQSGEFCHHDMANHNFLITEEGK
                     VYLIDFDYCIMDTRLHDLTSLVIRNMRYGIWDIGKAYFILNEYDRIYSISRKELEVIK
                     ALITFPQDFWQVGLQYYTEKQPWTMDYFLMRLNRIADDREIRDKFLKEFYEL"
     gene            4690..4911
                     /locus_tag="PKU88_11720"
     CDS             4690..4911
                     /locus_tag="PKU88_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69977.1"
                     /translation="MSGRKNEINEAIDIERGLRPEEQVNKLESFIKDMKEEIKQLKKI
                     GKHKEKIEKLEREKKKIISKKLKKHHRHH"
     gene            5335..6351
                     /locus_tag="PKU88_11725"
     CDS             5335..6351
                     /locus_tag="PKU88_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HPX69978.1"
                     /translation="MSGGYEKLKRHDLKLLIENLYGLEVRNMQEGDRGILVYTDRGIK
                     RLKGTESDEAKILFSASAYEHVYNNGFRQISCINRTLNGSCHMKYDKNNYILQDFTKG
                     RVYEANTAEAAAVAGKALAELHKAGEYFIPAPGSRARVDWGKWMEKFKANSISLKKYK
                     ELICLKEKKSEIDKLFLENIDKFLEKMQYAHGLLKESGYLDKVRQSMSCNQITHGEFK
                     KHAIFEGDPEGVFITNFEECSYDICEFDIATLLESFSGKNKDELAAAAAEAYSDVKPL
                     DEGSMKIIKAFLIYPKRFFKVSESIYGRRKNYNEIELAQKLARSIKREKKKEEVIRFL
                     DFGR"
     gene            6348..7412
                     /locus_tag="PKU88_11730"
     CDS             6348..7412
                     /locus_tag="PKU88_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HPX69979.1"
                     /translation="MNHLMRMELFMEENRQSDREFLGGYNLDVKLFEAMGFKVKQIIP
                     VRSVYRIVTDKGFYCLKRLRFPMEDMDFIFSALEHLKGKGFNNAYDIVKQENGDDFIS
                     FKGDKYFLTQWIDGRECDFMNPMDLDAAIEVLARLHNASEGFAPVVCPPDRCWYGKWP
                     EYFNDRIEEMKQMKEQVLEKPEKSEIDRIYLDYADMCINDGEEALQILSKTDYRSLSE
                     EAAVKKSFIHHDFAHHNIIHTFDGRIYAVDFDYCIMDIRMHDVGSLILRNMKKSNWDT
                     DKAMDILESYDRRNPVSSTELKVLVPFFLFPQDFWMISRQYYIERKDWDEEDFTDKMS
                     TKSEYTLVRRKFIEEFEKRI"
     gene            7501..>8053
                     /locus_tag="PKU88_11735"
     CDS             7501..>8053
                     /locus_tag="PKU88_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69980.1"
                     /translation="MKIDAIAREFGCRVIYSTPLRAVNLAQTDRGLVIIKETYRDPDK
                     ILYIHGLKEYLHKNGFTRIDRYLLSRYELPFAIYENRIFVMEDYIAGRECSFTNPYDR
                     EAIVKALAQLHNAGRGYLPSTGAAARNNIGKWEKSYREKIEDLTEFKGLAARKRKKGK
                     LDKMFLEDVDFYIEMGWRGFDTLK"
BASE COUNT         2563 a         1485 c         1855 g         2050 t
ORIGIN      
        1 gtccccctgt cctctccccg ttacctatgt cgcattattc gtctgtaacg aatcgttccg
       61 taccgcatat tataattatt acggaggttt gtaattatga actatgtaat aattatgtgc
      121 ggcggttcag gcagcaggtt ctggcccaag agcaggaaaa tgtaccccaa gcagtttttg
      181 aacaccgtag gagaaagaac catgatacag cttacagtgg ataggatcag caaatttatc
      241 cccctaaaga atatctatat ggtaactaac aaaaactacg tcaacaccat aagagagcag
      301 gtaccggaaa tattagaaaa gaatcttata ttagaaccaa tgataaagga gactgccgcc
      361 tgcattggat attcggcggt gaagcttctc aaaaaggatc ccgaagcggt tatgatagta
      421 cttccttccg atcactatat acaggatgaa gatatcttta tagagaccct gaaacagggc
      481 ctgcggatag ctgattcaga aaattgcctg gtaaccatgg gtataaagcc gacaagaccg
      541 gaaacggctt acggttatat tgaaacagga agaaaaatag aaggctcgtg ggagattcca
      601 acctacaaga taaaaagatt tacagaaaag ccaaacaggg aaaaggctca ggagtttatt
      661 gacaagggta cttttttgtg gaacagcgga atgtttatct ggaaggcatc ggtactgctt
      721 aagcagtata agaaatttct tcccgatatg tatcaatgtt tgaagagaat cagtgattat
      781 ataggaagcc cattggaggc taagatagtt gaagaagaat ataataaaat cgatggtatt
      841 tccattgact atggaatact ggagaagaca tgggatgtat atgtgatgga gagcagcttt
      901 tactgggatg acatagggaa ttggactgca atggaaagat atatgccgaa agacgagtgc
      961 ggcaacaccc ttaaggggga atgcaaggta ctggattcgc acaactgcat attgtacggg
     1021 gacaaaaggc ttattgcagt tctgggagtt gacgaccttg tgataataga gactgatgat
     1081 gtaatattag tgtgcagaaa agacagagat caggatataa agcttcttat taaggaattg
     1141 caagaggata aggaaaacat gaaatatata taggggcact taatagcgaa gtgtccctat
     1201 tcaattagca atcttgattt cttaatgtat ctgtaataca ttattccatg ataaattggc
     1261 agacttaacc agcctattcc aactgccagt gcaacaccgg caagaccgaa caccggtgaa
     1321 agtgaataag acaaaattat tcttataata atctgaaaaa atgttgcgaa aaatgtgata
     1381 ccgattttac ctattccacg gaaaaatcct tgaaaagcat agccaaatcc cgaaagaata
     1441 taaaacagag acattacttg taagtattgc gctcctaaag atataatgga ttggccttca
     1501 ttttctacaa atatgcccat aagaggatac gccccaaaat atactaagag tgaaatgctg
     1561 aagccgtaaa cagatagtaa tgaaaatccg gtctttagtc cgctcttgat tctatctttt
     1621 tttccggcgc ctgtgttttg agcgataaag gttgttaaag cgcttgctac gccgtctccg
     1681 ggggtcacaa aaaatgcgtc aattctggat actgctgaaa atgcggcaat ggcgcttata
     1741 ccaaggggat tgacagcgct ttggaccagc atttttccga tataaacgca agattgctgc
     1801 agagctgcta tccaaccgaa ttctgcagtt tttttcaaca gtttgagatc tatcattatt
     1861 tcttcttttt tgaatctcaa aataggatat gtatttagac catatattgc acacaataat
     1921 gcgcttgctg cctgcgaaat tactgttgcc aatccgactc caaagactcc catattcatt
     1981 ttaataatga atactaagtc cagtattata tgtagaattg atgctattat cagaaaaaaa
     2041 agagctatac gggagtttcc cattgaacga aatattgcat caaacagatt atacaacatc
     2101 gtaaaaataa gacctgaaaa aatagttata agataaaccc ttgcgtcatg catcagatta
     2161 tcaggggtgt ttaaccatct taaaattgaa tttgttacaa ttataaatgt tatgctgaca
     2221 ataattgaaa atatacttcc ggcaataaat gcagttgaaa tctctttttt tagttttttt
     2281 tggtcatttg caccgaatag ctgcgccatt aaaacagatg ctcctataca cattccaaca
     2341 atgatgaata gtgctgtgcc cattatcgga gaggcaacgc ctattgcggc aaatgcatcc
     2401 tctcctatgt acctgccgac cataatcgca tctacaatat tgtaaagctg ttgaaatata
     2461 tttccgatta taataggaat cgcaaaaaca attatatgtc tgtaaacatt gccttcagtc
     2521 atgctgtgca taatagttcc tcctcaggtt tgtcagttgt gtcaattatt ttaattatac
     2581 tatacatctt ccaaaaattc ttataataag acgaaattta cactataaac cccgtacctc
     2641 ccgcctctca cctcctgcct cttcatatac ctttatnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnntgct cataaggcac ataaccggca aataccaccc tgtcccttat
     2821 tcccattccc tcagtctttt tcttcaattc atagtgacta tccttggagg ctcccagcag
     2881 caccaggtcg taatatcccg gcagctcccg gcagattctc ctgtaggcct ccaataagcc
     2941 gtccacattc tttctcgggc tgaagccccc caggtataat atgaagtcgt tggaatagct
     3001 gtactccttc tttaagaaat cccatgcctt ttccttgtct ataagcctga acctgctgtc
     3061 tgcggccaga tatatgacct ttattttttc ttcaggcaga ttgaagtact ttattatatc
     3121 ctgctttgaa tagttggata ctgtaattac cctgtcgcac ttttccagta tgtagggcat
     3181 ttcctgcagg aacttatcca aatagctctt tccgcaggta tcgggcatta caaggggtat
     3241 gaggtcatgt acggtagcaa cgtaagagca ggtttttggc ctgggaagcc caaggccgtt
     3301 ctgaggcagg tggaatacat cgcagctgtt catttttacc tgggcgggga ggaatacctc
     3361 atcccagaac ttcttattct tttcgccaaa caaagtgagg ctgatattgc cccttcccaa
     3421 taagaaatca tatccgtcgt taggccagaa aaacctgtaa ttgttccggg tgtcaacttt
     3481 tattatattc tccagcaatc tcgctgtata tgtgcctatt cccgttccgg catagagctt
     3541 ggcgcctctt gcatccatgc atatattcat ataattcagc tccctatata tcagtatatt
     3601 agaacaccct tgtacctgtg aatagtcaaa gcagtaacac aatttttgcg gcgtcatata
     3661 atggtataaa tgctgttgag aggaatcatt atggatttaa aagcggtaga agagacctac
     3721 ggtattaaaa tcatagacat ccaaagcatc agaaatatcc tgagggcgga gacggattgc
     3781 ggagtaaagt gcattaagag ggcgcatatg agcccgggct ttttcctttt catatattcg
     3841 gcggtgaatc accttaagga aaagggatat gaaggggtaa tcccgtataa ccgggccatt
     3901 gacggaagca tatgcatacc tgacggcaag catgtctatt atgtggtgga ttggattgaa
     3961 gccagggagt gcaggttcag gaaggaagaa gagctgaaga tggcaataaa ggcagcggcg
     4021 gaactccaca gggcatcagt aggttatgtg cccccggagg gagcaaaacc aagggtattc
     4081 tataacaaat gggtggaaaa gtgtgaaaag aaaagcaagg aactgctgga attcggcaag
     4141 gcaatagaag ataaggagca tagggacgac tttgacgaaa tatttgccct tcatctcccg
     4201 tattatttgc agcaatccct ggatacagca aatatgataa tggaaagccc atattgggaa
     4261 atatcggaga aaagtgagca gtcaggggaa ttctgccatc acgatatggc aaaccataac
     4321 ttcctgataa ctgaggaggg caaggtttac cttattgact tcgattactg cataatggat
     4381 accaggctgc atgaccttac gagcctggta ataaggaata tgagatatgg aatctgggat
     4441 ataggcaagg catatttcat actgaatgaa tatgacagaa tatatagcat aagccggaag
     4501 gagcttgagg taataaaggc attaataacc tttccgcagg atttttggca ggtagggctc
     4561 cagtattata ccgaaaagca gccctggacc atggattatt ttctgatgag gcttaacagg
     4621 atcgcagatg acagggagat tagggataag tttttaaagg aattctatga gttgtaagga
     4681 gttgattttg tgagcggcag aaagaatgaa atcaatgaag caatagatat tgaaagaggc
     4741 ctgaggcccg aagagcaggt caataagctg gaaagcttca taaaggatat gaaggaagaa
     4801 ataaagcagc ttaaaaagat aggcaagcac aaggaaaaaa tagaaaagct tgaaagggaa
     4861 aaaaagaaaa ttatcagcaa gaagctgaaa aagcatcaca ggcatcatta gaagaatgcg
     4921 atatatctga agtttgcata acccaccgca agagacagta tatgagacac gggggacgtt
     4981 tcgcgtgtct cgctttcagt gagacaggag aaacgtcccc atgtctcgaa gtgtgccctg
     5041 ctatactata catgctcttg cttggttatg cagacttaca ggatattgcc ttatataaag
     5101 gatatgcaaa taccgacgtg gggaagctat attcgacgaa accttccacc tgaccccgga
     5161 acgaaaaaac ttctggcaaa ataaccagac gggcttgcag agatgggacc ctcgccagtg
     5221 attttgctgg ctttggaagg tgaggagaac atacttcccc tacagtcgga gtactttaca
     5281 aacctgtata tattgcgcaa tattcctcaa ttacgtctta ggaggggata gttaatgtcc
     5341 ggcggatatg aaaagttgaa aaggcatgat ctcaagcttc tgatcgaaaa cctttacggc
     5401 ttggaagtaa gaaatatgca ggaaggggac agaggtatac ttgtctatac cgacagagga
     5461 ataaaaaggc ttaaggggac cgaaagcgat gaggcgaaga tattattctc agcatcagct
     5521 tatgagcatg tctacaataa cggtttcaga cagatcagct gcatcaaccg gaccctgaac
     5581 ggcagctgcc atatgaaata tgataagaac aactatatac tccaggattt taccaaggga
     5641 agggtatatg aagcaaatac agccgaagct gctgcagttg ccggcaaagc cctggcagag
     5701 cttcataagg cgggagaata cttcatacct gcgccgggaa gccgtgcccg tgttgattgg
     5761 ggaaagtgga tggagaaatt caaggcaaat tccatcagct tgaaaaagta taaagagctt
     5821 atttgtctca aggaaaagaa aagtgaaatt gacaagctgt ttttggaaaa tatagataaa
     5881 ttcctggaaa aaatgcaata tgcccatggg cttctgaagg aaagcggata tcttgacaag
     5941 gtgaggcagt ctatgtcatg caatcagata acccatggag agttcaagaa gcatgcgata
     6001 tttgaaggcg atccggaggg ggtgttcata accaacttcg aggaatgcag ctatgatatc
     6061 tgtgaatttg acatagcgac gcttcttgaa agcttctcgg ggaaaaacaa agatgagctt
     6121 gcggcagcgg cagcggaagc atattcagat gtaaagcccc tggacgaagg ttccatgaag
     6181 ataataaaag cctttctcat atatcccaaa agatttttca aggtttcgga gagcatttac
     6241 ggaagaagga agaactataa cgaaatcgaa ctggcacaaa agcttgcgag aagcataaaa
     6301 agggaaaaga aaaaagagga agttatcaga tttctggatt tcggacggtg aatcatttaa
     6361 tgaggatgga gctttttatg gaggaaaaca ggcagtcgga cagggaattt ctcggaggat
     6421 acaatctgga tgtaaagctt tttgaggcta tgggctttaa ggtcaagcag ataattcccg
     6481 tgagaagtgt gtacagaata gtaaccgaca aggggtttta ctgtttgaaa aggctaaggt
     6541 ttcccatgga ggatatggat tttatttttt cggccttgga gcaccttaag ggcaagggct
     6601 tcaacaatgc ttatgatata gtcaagcagg aaaacggtga tgactttata agcttcaaag
     6661 gggataagta tttccttacc caatggatag acggaaggga atgcgacttt atgaacccta
     6721 tggaccttga tgctgccatt gaagtgcttg caaggctgca caacgcttca gagggctttg
     6781 cccctgttgt atgccctcct gacaggtgct ggtacgggaa atggccggag tatttcaacg
     6841 ataggattga agaaatgaag caaatgaagg aacaagtgct ggaaaagccc gaaaagagtg
     6901 aaatagacag aatatatctt gattatgcgg atatgtgcat aaatgacgga gaggaagcgc
     6961 tgcagattct gagcaaaacc gattacagga gcctttccga ggaagcggca gtgaagaaaa
     7021 gctttataca ccacgatttt gcccatcata atataataca tacctttgat ggccgaatat
     7081 acgcagttga ttttgattac tgcattatgg atataagaat gcatgatgtg ggaagcctta
     7141 ttctgagaaa tatgaagaag tcaaactggg ataccgacaa ggccatggat atactggaaa
     7201 gctatgaccg gagaaatccc gtaagcagca cggaactcaa ggtacttgtt cccttcttcc
     7261 tttttcctca ggacttctgg atgatatcca ggcaatacta tatcgaacgg aaggactggg
     7321 atgaagaaga ctttacggat aaaatgagca caaagtcaga gtatacatta gtgagaagga
     7381 agtttataga agagttcgag aaaagaatat agtatcaaag aaacaacggt ataagtctcc
     7441 gcagcccctc atatattcta atagtatgtc agctaatttg tatactatga ggtgctgcca
     7501 atgaaaatag atgccattgc cagggagttc ggctgcaggg tgatatacag cacaccgttg
     7561 agggcggtca atctggctca aaccgacagg ggattggtaa taataaagga aacctacagg
     7621 gatccggata agatattgta tatacacggc ttaaaggaat acctccataa gaatggattt
     7681 acgcgtattg acaggtatct gctgtcccga tacgagctgc cctttgcaat atacgagaac
     7741 aggatattcg tgatggagga ctatatagcg ggaagggaat gcagcttcac caacccctat
     7801 gacagggaag cgatagttaa ggcgctggca cagctgcata atgcaggcag gggatatttg
     7861 ccttctaccg gtgctgcggc gagaaacaac atcggaaaat gggaaaagtc ctatagggag
     7921 aaaattgagg atctgacgga attcaagggg cttgcggcaa ggaaaaggaa aaagggaaag
     7981 ctggataaaa tgttcctgga ggatgtggac ttttatattg aaatgggctg gagaggcttt
     8041 gataccctga aga
//
LOCUS       DAOOAB010000076         7781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_11280_length_7781_cov_15.439166, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000076 DAOOAB010000000
VERSION     DAOOAB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11280_length_7781_cov_15.439166"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKU88_11740"
     CDS             <1..492
                     /locus_tag="PKU88_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPX69981.1"
                     /translation="EDVDFYIEMGWRGFDTLKNSNYADICKKAKKDNVICHHDYTYHN
                     IIIGPGGEVNVIDFDYSCHELPVYDLAALIQKVLKRYSYDVDMALEIIEDYCSIAPLE
                     REDLVLMLSLFEFPQRFWRIAERYYKGKTTWDEKTFLAKYNDTVIMKEFILDFVEDFR
                     KYI"
     gene            848..1714
                     /gene="yabG"
                     /locus_tag="PKU88_11745"
     CDS             848..1714
                     /gene="yabG"
                     /locus_tag="PKU88_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HPX69982.1"
                     /translation="MDIKVGDIVARKSYGFDILFKVHDIVTNKETGTIIYLKGLHYRL
                     FADAPESDVVKVPMNRVNEEIHRFEGKIGRLTKARGRRAGFLSKKLLPKREEYLKFEL
                     PGKVLHIDGDKDYLVMSMKYYKEQGISAVGKLVPEADQPGMIKALLEEYKPDIVIITG
                     HDSMQKQEAKGKDSSERYRNSKYFIEAVKEARKYQQSLDELVIIAGACQSYYEGILSA
                     GANFASSPARILIHALDPGMLCRQIAFTPTDTAAPIEDVLKGTTSGVKGIGGIQTRGK
                     FRAGMPGSPYIP"
     gene            1731..3110
                     /locus_tag="PKU88_11750"
     CDS             1731..3110
                     /locus_tag="PKU88_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPX69983.1"
                     /translation="MLFKVYDCEKELSYDTELDELTARITSFNTAEGHEVEYLKALAV
                     MARTELARKTFIYNGKGCERHRGCDICTEPGHCLEYGLADTEIPKGVYDAVASTDRII
                     MLFEGRPIKPFFHYRCGGATENSENVLGNRITYLRRVLCSFCKDNTDNDSDRYFTVTE
                     LEGLLKTRLKKPEGIYCNIRGMFEDVEVDEQGKISRIKIGTKSFRGIEVRELLKLNST
                     RFDYIPVKFLIKCIGTGHGLGLCQCGANSMARSGMSYQEILKYYYTGIRFEQMEVPDS
                     ERPLKGVRIVLDAVHGGEDCDEGNANLDIVLKLKSLLEGQGAEVYLTRSSGEEMVLSD
                     RAAISNDKRPDLFLSIGQNCFSNPTASGTEVYYYRGDSQGEKLSKLIMENVSGSLGLK
                     NRGVRMADFYLLREIKASSVIIQLLYTSNPQDKELLCNQSFRESAAAAIFKGIGAYYE
                     NSRSGAAAH"
     gene            3356..3628
                     /locus_tag="PKU88_11755"
     CDS             3356..3628
                     /locus_tag="PKU88_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HPX69984.1"
                     /translation="MASKNTLSLIKSNIDNYVGERVVLRANKGRKKISIKQGVIEKTY
                     PSIFLVRIEGEVPEDFGRTVSFSYSDILTKSIELFACKDNVKIGCM"
     gene            4014..5252
                     /locus_tag="PKU88_11760"
     CDS             4014..5252
                     /locus_tag="PKU88_11760"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HPX69985.1"
                     /translation="MFYEEREPFLNVTSEIGKLKAVLLHKPGKELERLTPDYLKDLLF
                     DDIPWLRKMRDEHDEFADVMIQRGAKVYYIEDLLAEILTDLDVKRNLINDILINSNIE
                     NHELRELIFSYIFSKTPAEAAEIAIAGLNKKDIQDVDREYSLSDYIRKDYPLYINPIP
                     NLYFMRDPLSIIGNGISINSMQTNARRREPMLIKYIYDYNPLFRKEYSQVWYNYTIPH
                     SIEGGDILVLSKEVVAVGCSERTSANGIEMLARNLFAGDNDIKEVLAVQIPPLRAFMH
                     LDTVFTMVDRDKFTVYPGILDRVDVYRLTPGGRNGIKVTPENDLVAALKKSLKLPAVS
                     LIQSGGGDTITAAREQWNDSTNTLAIAPGVVVTYSRNEASNEVLRKHGIEVLEIDGSE
                     LVRGRGGPRCMSMPLVREDI"
     gene            5281..6267
                     /gene="argF"
                     /locus_tag="PKU88_11765"
     CDS             5281..6267
                     /gene="argF"
                     /locus_tag="PKU88_11765"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPX69986.1"
                     /translation="MAVNLKGRSFLTLMDFSPLEIRYLLDLSRDLKAKKRAGIYNYLL
                     KGKNIVLLFEKTSTRTRCAFEVGALEEGAHVTYLDQSGSQMGKKESLEDTAKVLGRFY
                     DGIEYRGFKQSAVEDLAKYSGVPVWNGLTDVDHPTQILADMLTIEEHVAKPLNKVKVV
                     FCGDTRNNMAYAWMYGCAKMGMHFTALGPEELSPSQDILNKVNEVAKETGAVIEVAHD
                     IDAVKDADVIYTDVWASMGEEDKIPERVKLLTPYKVTMEMLEKTGNPDVIFMHCLPAF
                     HDFETKLAKEMKEKGYDIREVTDEVFRSRHSVVFDEAENRMHTIKAIMVATL"
     gene            6375..7319
                     /gene="arcC"
                     /locus_tag="PKU88_11770"
     CDS             6375..7319
                     /gene="arcC"
                     /locus_tag="PKU88_11770"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPX69987.1"
                     /translation="MSVIKVVVALGGNALQESGKPATAEEQLQVVNKTAEYLAEMSIK
                     GYEMAVVHGNGPQVGRILLASETAAGVTPVMPFDVCGAMSQGYIGYHIQQGLREALRK
                     RGRNIPVVTLVTQVVVDKDDPGFKNPTKPIGAFYTEEEAKRLQAEKGYVMKEDAGRGW
                     RRVVPSPMPKKIVELSAVKTLWDTTIVITAGGGGIPVIENEDGSLTGIAAVIDKDLAA
                     ERLAEDINADILMILTEVERVSINYKKPDQKDLSLLTSAEAEQYIREGHFAPGSMLPK
                     IQAAVSFVKANPGRKAIITSLYKAVDALEGNAGTTIIF"
BASE COUNT         2484 a         1401 c         1960 g         1936 t
ORIGIN      
        1 gaggatgtgg acttttatat tgaaatgggc tggagaggct ttgataccct gaagaattca
       61 aactatgcgg atatatgcaa gaaagcaaaa aaagacaatg tcatatgcca tcatgactac
      121 acttatcaca acattataat cgggccggga ggggaggtaa acgtaataga ttttgattac
      181 agctgccatg aactacccgt ttatgacctg gctgcattga tacaaaaagt actgaagcgg
      241 tacagctatg atgtggatat ggctttggag attatagagg attactgcag tatagcgcct
      301 cttgagagag aggacttggt gctgatgctg tcgctttttg aatttcccca gaggttctgg
      361 aggattgccg agaggtatta taagggtaaa accacatggg atgagaagac ctttttagcc
      421 aaatacaacg ataccgttat aatgaaagaa tttatcctgg actttgtgga ggacttcaga
      481 aagtacatat aacgcgagaa ttcagccgtg cctcgtgact gataaaaacc agcaaaaata
      541 ttgttcgaat cgctgtcaaa ggtcgtccgt gaccgtgaca gcttgctcgg cctccatgcc
      601 tcgcatacgg ttgtttctaa gtcgagcgag atttgagcca ttaacaattt ctgtataagt
      661 atgctctgac tatacattgt tgtccctatt ctgagctttg cctgcaaact gaaacagtaa
      721 aaaatactgt tttttatttt ttgctcacct tgtcagcctt aatcacatac tatgtattaa
      781 actatacaaa atcggttcaa gctccttgcg tttcaaaccg taagctttaa taacaggggg
      841 tcgggatatg gatatcaagg tgggagatat agtagcaaga aagtcctatg gctttgacat
      901 actgttcaag gttcatgata tagtcaccaa caaagagaca ggcaccatca tataccttaa
      961 gggcctgcac tacaggcttt ttgccgatgc acccgaatct gacgtcgtta aggttcccat
     1021 gaacagagtg aatgaagaga tacaccgctt tgaaggcaaa atcggcaggc tgaccaaggc
     1081 caggggaaga agggccgggt tcctgtcaaa aaaactgctg cccaaaagag aagaatatct
     1141 caaattcgag ctgcccggta aggtgcttca tatagacggc gataaggatt atctggttat
     1201 gagcatgaag tattacaagg aacagggcat cagcgccgta ggaaagcttg tgccggaagc
     1261 agaccagccg gggatgataa aggctttgct ggaggagtat aagccggata tagtgattat
     1321 cacagggcat gacagtatgc agaagcagga agccaaggga aaggattcat cggaaaggta
     1381 caggaattca aaatatttca tagaagcagt caaagaagcg agaaaatacc agcagtcact
     1441 ggatgaactg gtaataatag caggagcatg ccagtcatat tatgaaggca tattgtctgc
     1501 aggggcaaat tttgcaagtt cccctgccag aatattgata catgccctgg accccggaat
     1561 gctgtgcagg cagatagcgt ttaccccaac tgatacggca gcccccatag aggatgtgct
     1621 gaaaggcacc accagcgggg taaagggaat cggaggcata cagacaagag gaaagttcag
     1681 ggcggggatg cccggttctc cctatatccc atagaagggg aggaattagg atgttgttta
     1741 aagtgtatga ttgtgaaaaa gagctgagtt atgacacgga actggatgag ctgacagcca
     1801 ggataacaag cttcaatacg gcagaagggc atgaagttga gtatctgaaa gctcttgccg
     1861 ttatggcaag aacggagctg gcaagaaaaa cctttatata taacggtaaa ggctgcgaga
     1921 ggcacagagg ctgtgatata tgtacagaac cggggcattg ccttgaatac ggccttgccg
     1981 atactgagat accaaagggg gtttatgatg ctgttgcttc aacagacaga atcataatgc
     2041 tttttgaagg aaggccgata aaaccttttt ttcattaccg gtgcggcgga gcaaccgaaa
     2101 actccgagaa tgtcctggga aacaggataa cttatctgag aagggtattg tgttcttttt
     2161 gcaaagataa taccgacaac gacagcgaca ggtatttcac agtcacggaa ttggaggggc
     2221 ttttaaaaac caggctcaaa aagcccgagg gtatttactg caatatcagg ggaatgttcg
     2281 aggatgttga agttgatgag caaggtaaaa taagcagaat aaaaatcggc acaaaaagct
     2341 tcagagggat agaagtaaga gaactgctga agcttaactc caccagattt gattatatac
     2401 cggtaaaatt cctgataaag tgcatcggta ccgggcacgg gctggggctg tgccagtgcg
     2461 gggcaaattc aatggcgagg tcaggaatga gctatcagga gatactgaaa tactattata
     2521 caggaataag gtttgagcag atggaagtac ccgattccga aaggccttta aagggtgtca
     2581 ggattgtatt ggatgcggtt catggcggag aggactgtga tgaaggtaat gccaacctgg
     2641 atatagtttt aaagcttaaa agccttcttg aaggccaggg tgccgaagta tatctgacaa
     2701 ggagcagtgg cgaagaaatg gtgctttcag acagagcggc catatccaac gacaaaaggc
     2761 cggatttgtt cctgtctatc ggccaaaact gcttttccaa tccgacagct tccggcactg
     2821 aagtatatta ttacagagga gattctcaag gagagaagct gtcaaagctg ataatggaaa
     2881 atgtcagcgg ctctctcgga ctaaagaaca gaggggtgag aatggctgat ttttatctgc
     2941 tccgggagat taaggcatct tctgtgataa tccaactgct ttatacaagc aatccgcagg
     3001 ataaagagct tttatgcaat caaagcttca gggaatctgc ggcagcagcg atatttaagg
     3061 gaatcggggc atattacgaa aactctcgca gcggagcagc tgcacattag aataaaattg
     3121 cgcagcaatt ttcttttttg ttcttttttt gagtaatatt gagtaattcg gggaataata
     3181 ctgtatagtt tatacttaat agcttgtaat gatgaaaaat agccgaaaaa tagctgaaaa
     3241 ataaaaatag ttatttgact attaaagtat aagttgatat aatattttgt tccagttgac
     3301 atataatata tgttatgata aaatttaaga tgttgggaag gaaggtgatt tttttatggc
     3361 ttcaaaaaat accctatccc tcatcaaaag caatatagac aactatgtcg gggagagagt
     3421 cgtacttaga gctaataaag gcagaaaaaa gataagcatc aagcaaggtg tgatagaaaa
     3481 gacatatcca agtatatttt tagtacgaat tgaaggagag gtacctgagg acttcgggag
     3541 aacagtttca ttcagctatt cagatatact gacaaaatct atcgagctat ttgcatgtaa
     3601 ggataatgta aagataggat gcatgtaata tatatagctt tgtaaggcaa aaattgagac
     3661 ccaatcaggg ttcttaattt tttttgtctt tttttgcgtt tgaaataaat aattttttat
     3721 tccaatatct aaattattat gcatacaatg cataaataca gtaaagtcta tcaaatgagt
     3781 gtaaaggagt ttgaataagg ataatggcaa tattgcacgg acacctaata attattcaca
     3841 gtgcatttga attaatatac aagcgtatgg gataaaatgg gaaaagccgt cttaatatag
     3901 agcttaagcc acttgtacag ttatttctat ttgtatatac taatattggt aataatgagg
     3961 cgagaagatg tattccaatg cggtatatcc accttttgaa aggagagtaa cagatgtttt
     4021 atgaggaaag agagcctttc ctgaatgtga catccgaaat cggcaaatta aaagctgttc
     4081 tgctgcataa accgggtaag gaattggaga ggcttacccc ggattatctc aaggatcttt
     4141 tgtttgatga catcccatgg ttgaggaaga tgagggatga gcatgatgaa tttgcggatg
     4201 ttatgataca gagaggcgca aaggtctatt atatcgaaga tttgcttgca gaaatactga
     4261 ctgatttaga tgttaaaaga aacctgatta atgatatact gattaacagc aatatagaaa
     4321 atcatgaact gcgcgaattg atattttcgt acatattcag caaaacccct gcggaagcgg
     4381 cagagattgc catagcaggg cttaacaaaa aagatattca agatgtggac agggaataca
     4441 gcctctcaga ttacataaga aaggattatc ccctatatat aaacccaata ccgaatctat
     4501 attttatgag agatcccctg tctataatcg gtaacggaat ttcaatcaat tccatgcaaa
     4561 caaatgcaag aagaagagag cccatgttaa taaaatatat atatgactac aatccgcttt
     4621 tcagaaaaga atactcacaa gtatggtata attacaccat accgcacagc atagaaggcg
     4681 gagatatcct ggtgctcagc aaggaagtgg tggcagtggg ctgcagcgaa aggacttcgg
     4741 ccaacggcat cgaaatgctt gcaaggaatc ttttcgccgg ggataatgat atcaaagagg
     4801 tgctggcggt tcagatacct cctctaaggg catttatgca tctggatacg gtatttacaa
     4861 tggttgacag ggataagttc acagtgtacc cgggaatact tgacagagtt gatgtttaca
     4921 ggcttactcc cggcggcaga aatggtataa aggtcacacc ggagaatgac cttgtggcag
     4981 ctcttaagaa gagcctcaag cttcctgcgg taagcctcat acaaagcggc ggcggagaca
     5041 cgataacagc cgcaagggag caatggaacg acagtaccaa tacattggca atagctcccg
     5101 gtgttgtagt aacttattca agaaatgaag cctccaatga agttcttaga aagcatggga
     5161 ttgaagtgct ggaaatagac gggtcggaac tggtaagagg cagaggaggc ccaaggtgca
     5221 tgagcatgcc tttggtcagg gaagatattt agaataatgt tttaaaggag agattttaca
     5281 atggcagtca atcttaaagg aagaagtttt cttacattaa tggatttttc acccttggaa
     5341 atcaggtact tgttggacct gtcccgtgat ttgaaggcta aaaaaagagc gggcatatat
     5401 aattatctgc tcaagggaaa gaacatagtt cttctttttg aaaaaacctc tacaagaaca
     5461 agatgtgctt ttgaggtggg agcccttgaa gaaggagccc atgtaaccta tcttgaccag
     5521 tccggctcac agatgggtaa aaaggaatcc ctcgaagata ccgcaaaggt actgggaagg
     5581 ttttatgacg gtatagagta cagaggcttc aagcagtcgg ctgtggagga tcttgccaaa
     5641 tattccggag tacctgtatg gaacggcctt acggatgtag accatccgac acagatactt
     5701 gcggacatgc ttacaattga agagcatgtt gcaaagcccc ttaacaaggt taaggttgta
     5761 ttttgcggag ataccaggaa caatatggca tatgcatgga tgtacggctg cgcaaaaatg
     5821 ggcatgcact ttacggcatt gggaccggag gagctgagcc ccagtcagga tatattgaat
     5881 aaggtgaatg aagttgccaa ggagactggt gcggttattg aagtagctca tgacattgat
     5941 gccgtaaagg atgcggatgt catatacacg gatgtatggg cgtccatggg cgaagaggat
     6001 aagattccag aaagagttaa attgcttaca ccatataagg ttacaatgga aatgcttgaa
     6061 aaaaccggca atccggatgt catattcatg cactgcctgc cggcattcca tgatttcgaa
     6121 acaaagctgg caaaggaaat gaaggaaaag ggatacgata tcagggaagt gactgatgag
     6181 gtattcagaa gcaggcattc tgttgtattt gacgaagcgg aaaacaggat gcatacaatc
     6241 aaagccataa tggtggcaac attataaaga tagatacgag gtacgggata cggggtacga
     6301 ggagttgagt taggtcttcg aagacaggct caagtctcgg aaactaaaat gttttttgtg
     6361 aaggggaagg atttatgtca gttataaagg tcgtggtagc attaggcgga aatgccctgc
     6421 aggagtccgg aaagccggca accgcagaag aacagcttca ggtagtgaac aaaacagctg
     6481 agtatcttgc ggaaatgagt atcaaaggat atgaaatggc agtagtacac ggcaatgggc
     6541 ctcaggtggg aagaatactt ctggcatcgg agactgccgc gggagtcact cctgttatgc
     6601 cttttgatgt ttgcggggca atgagccagg gctatatcgg atatcatata cagcagggct
     6661 taagagaggc actgaggaag aggggcagga acatacctgt agtaacattg gtaacacaag
     6721 tggttgttga taaggacgat ccgggcttta agaacccgac taagccaatt ggtgcttttt
     6781 atactgaaga agaggctaaa aggcttcagg cagaaaaggg atatgtgatg aaggaagatg
     6841 cgggaagagg ctggagaaga gtcgtcccat ccccgatgcc caagaagata gttgagcttt
     6901 cggcagtaaa aaccctctgg gatacaacca tagtaattac ggcagggggc ggaggaatcc
     6961 ctgtaattga aaatgaagac ggcagtctta cgggcatagc ggcagtaatt gacaaggacc
     7021 ttgccgcaga aaggcttgcc gaggacatca atgcggatat acttatgata cttacggaag
     7081 ttgagagagt ttcaatcaac tacaagaagc ctgatcagaa ggatctttcc cttctgactt
     7141 ctgcagaagc ggaacaatac atcagggagg ggcatttcgc accgggcagc atgcttccga
     7201 agattcaggc cgctgtatcg ttcgttaagg caaacccggg aagaaaagcg ataatcacct
     7261 ccctttacaa ggcagtggat gctttggagg gcaacgcagg aacaacaatt atattttagt
     7321 aggggcgcca tacatggcgt ccttcttaag gacacgggga cgcggggacg tttcgcgtgt
     7381 cccactggaa gtgggacagg ggaaacgtcc ccatgtcccc gggggaaacg tccccgcgtc
     7441 ttttttgcat acgtagaata atgcgcacta acgtgcgcag gcaccattaa ctagacacca
     7501 gaaatccggg tattccttat gtgtaacatg taacactgaa aacactgaaa gccactaaat
     7561 tcacggtggg ataaatatta cgttacataa ataactatcg ataatgatat aagtatcgaa
     7621 atcttcttat gcagctgcat aatatataat agttaccgtg taatcgcgca gcgattccgt
     7681 gaaacagccg tgtgtttagt gttacccgtt acctatgtca cattcttcat atattgcgca
     7741 acagccccta aaataaaaat gttggcataa attccttctc g
//
LOCUS       DAOOAB010000077         7237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_12323_length_7237_cov_15.595795, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000077 DAOOAB010000000
VERSION     DAOOAB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_7237_cov_15.595795"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..4234
                     /locus_tag="PKU88_11775"
     CDS             146..4234
                     /locus_tag="PKU88_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPX69988.1"
                     /translation="MEKSFSSKALEVNLAKTRDTDILIPEEHKWFAELSKSYWGIYKR
                     TQEFIKELNHQYINYQYVIENLHNISLTDLWFYNSLEESEKALAVLVDIFKKLLEANL
                     KENQRELLITTLIKFIDRLAKLGEYPESIIHQCLGIIKADMGKHELLYIRNSGYFKTY
                     LNKIAELPQYNRIVLEITGNLLSKCIDYWEETSEVGKWFQEKRSLFHTDNSDKIKDIG
                     KPFFEGLRMQLSKASDWSRMCEMLFFNDISNYFRRFSEQFDTSIERIYYIFYLIHLPG
                     MLQLKNHLLYDMNRLLRNVLKELDENETTTFLTNIMALFGELKEQHTGTVLECIQTLG
                     KEVIDTGDKRIISYFVKGLIEYGFVYPGELVVNSDWQIQVNTNHVKNIRVWLELIEYS
                     PEDMKELLSSLIVNLKLGGIFISDTDLFQRDVTKLLNSNIEPVYKQMKQLARIFPVYF
                     RDIGAEGKLREVTTAVDELSRRKDKLIHFLRKQIHTESNNTHIELAKKIIQYWYDGNP
                     EPLRKVVPEDVFEFLDTSSEWYIHVHEILTELCRMKDADPEQLLLLDVKELEQSISAI
                     GSESSRDKRRVAYLIQLYLLLLEKYSLESENIVLMLKGYRFFSNKDIEELRQGLDRND
                     IREALGQVYKLMRHLKKIIIDKNTSEALENIYYKRHVAIGIPSMYGQYIEPKFEALGL
                     MFRLEKAASKMMVQLLQDINLEYISAKTFRDVYDVLGLFKEGLELDGIYNQGFNSNFE
                     MFKYSLTSPSFSLDQYIDIFQFMAQNIKQIINEYFLDVYESPLKVAIPQIFDIKGPLS
                     DPKNKQLYHMESERFFREILSSAFLVQDLDNFITNLVSTLRSMVDNYSGDIIKSMMTY
                     DPDLTISPLYMETVEMDNPVFLGAKAYFLKKMISYDFPIPYGFVLTTEVFRHRRIITE
                     HPYMEQEIDQLILNHIWELEKITRQQFGNPKNPLLFSVRSGTAISMPGAMRTFLNVGM
                     NDEIAETFSKKPNHSWTAWDCYRRFIQSWGMAYGIERDVFDGVILEHKVKFGVEQKIQ
                     FTAEQMKEIAYAYKKVLGDYGIHIEKDPFKQLKQAILSVIDSWSSGRAIYYREHLQIA
                     DEWGTAVIVQKMALGNLSERSGTGVVFTNSPLKTKSGINLYGDFTLCSQGEDIVSGLV
                     HTLPITESQRRDYYSDCSISLESAFPDIYNALLDLAKQLIETYGFMHQEIEFTFESEN
                     PDDLYILQTRNQKLKKQKRLSSFIPAPDEMKLAGRGIGIGGGALTGILTFDMEDLKEA
                     ANKHPEEKRILVRPDTVPDDIPLIFSSDGLITGKGGATSHAAVTAASLGKVCVVNCKG
                     LIVNEAEKRCSINGISLKSGDRISIDGNLGNVYEGVYEIQYE"
     gene            4370..5623
                     /locus_tag="PKU88_11780"
     CDS             4370..5623
                     /locus_tag="PKU88_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPX69989.1"
                     /translation="MNAPLQSKRMLGINGIGRIGKLTLWNHLNLKHFDGIVINAGREV
                     GKKADDIIHYLTTDSTYGTVDRFLYGYTGKSCNVKVLDETECVFEMNGMPVRILRKER
                     NPRNIDWSREGVRLVVDCTGVFLDPTQPSDSPKGSIRGHLEAGAEKVIASAPFKIKDS
                     SQRMPDDSALFVYGVNHASYDPARHHILSAASCTTTGLAHMIKPLLDTEETSRIITAS
                     MSTVHAATNNQNILDAPPKAGSKDLRRNRSVFNNIIPTSTGAAIALEEILPEIKRVGF
                     MADSVRVPTSTVSLISLNITFRTDLNLTGEPIINREFINDIYRKAASGAQKGLLIFSE
                     EQNVSSDLMGCQAAVIIEGSENHTRTGFLPLSSEVLREYGIDTAQDINLPVTHAKIFG
                     WYDNEFGNYVNCLGKLTVYVDKNMI"
     gene            5872..7149
                     /locus_tag="PKU88_11785"
     CDS             5872..7149
                     /locus_tag="PKU88_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX69990.1"
                     /translation="MIIHVVRAGDSLYSISRRYGVPVERIASDNELNVSESLVIGQTI
                     VVMEGTRRHTVRAGESLYSIARQYGTTVAGIQAANQIANPAMIRPGMVLTIPSGTDKL
                     GTIDVNGYAFPNISMDVLRKTLPYLTYLSIFSYEVKPDGSFRPINDEPLIAAARAANV
                     APLMVITNLREGGGFDSDLAKTILTNEQVQDTLINNVIENLRAKNYTGLDIDFEYVYP
                     ENREDYNNFLRKVSSGLRPLGYSITTALAPKIAADQPGLLYEAHDYPVHGALVDHVIL
                     MTYEWGYTFGPPMAVAPINEVRKVINYAMSAIPRRKILMGIPNYGYDWVLPYVEGSRA
                     TTISNVGAVDLARREGAAIQYDQTAQAPFFNYYDDNGRQHVVWFDDARSMEARLRLVN
                     QYNLGGVSYWTIGRFFPQNWLVLSSLYDVRKVL"
BASE COUNT         2274 a         1350 c         1742 g         1871 t
ORIGIN      
        1 gaaacgtccc cgtgtccctg tccccatgtc tcattcagat gtacaatcat aaatttttac
       61 aaatttatga tataatatac tcatatcata gttttatatt ttgcagcaaa ctacatctta
      121 atattaaatt ctggaggatg accaaatgga aaaaagtttt tcttctaaag ctctggaggt
      181 aaaccttgca aaaacacgag atactgatat tcttattccc gaagagcata agtggtttgc
      241 ggagctttcc aagtcatact ggggtatata caaacgcact caggaattta ttaaagagct
      301 taaccatcaa tacatcaact atcaatatgt aattgagaat ctgcataata tcagcctgac
      361 agacctgtgg ttttataatt ctctggagga gtccgaaaag gcacttgcgg tgctggtgga
      421 catattcaag aaattgttgg aagcaaatct taaagagaat cagagagaac ttctgattac
      481 aactctaata aagttcattg atcgtcttgc aaagctgggg gaatatccgg aaagcattat
      541 tcaccagtgc ctgggaataa tcaaggccga tatgggaaag cacgagctgt tatacataag
      601 gaactcaggg tatttcaaga catatctgaa taagatagcc gagcttccgc agtataaccg
      661 gattgtgctg gaaattacgg ggaatttgct ttctaaatgc atcgattatt gggaagagac
      721 ctcagaggtg ggaaaatggt tccaggaaaa gagatcactt tttcatacag ataacagcga
      781 taagataaag gacatcggga agcctttttt tgaagggtta aggatgcagc tgagcaaagc
      841 ctctgactgg agccggatgt gtgaaatgct cttttttaat gatatatcca attacttccg
      901 acggttcagt gagcaattcg atacttccat tgaaaggata tattatatat tttatcttat
      961 acatctgccc ggaatgttac agcttaagaa ccatctgctt tatgatatga acaggctgtt
     1021 aaggaatgta ttgaaggaac tggatgaaaa tgagacgaca accttcctta ccaatattat
     1081 ggctttgttc ggggaattga aggagcagca tacgggaacg gtattggaat gtatacagac
     1141 cctggggaag gaagtcattg ataccgggga taagagaata atctcttatt ttgtcaaggg
     1201 attgatagaa tacggatttg tgtatcccgg agagcttgtt gtaaacagtg actggcagat
     1261 acaggtgaat acaaatcatg tgaaaaacat cagagtctgg cttgaactta ttgaatactc
     1321 acctgaggat atgaaggagc tgctttcgtc ccttatagtc aatctgaagc tgggaggaat
     1381 tttcatatcg gataccgatc ttttccagag agatgttacc aagctgttaa actccaatat
     1441 cgaaccggtt tataaacaga tgaagcagct ggccaggatt ttcccggtgt atttcaggga
     1501 catcggagcg gaaggaaagc tgagagaggt gactaccgca gttgatgagt tatcaaggag
     1561 aaaggataag ttgatacatt ttttaaggaa gcagatacat acagagagca acaatactca
     1621 catagaactt gcaaaaaaga taatacaata ctggtatgac ggaaatcccg agcctttaag
     1681 gaaggttgtg ccggaagatg tttttgagtt cctggataca agcagcgagt ggtacataca
     1741 tgttcatgaa atacttacgg agttatgcag aatgaaggat gccgatcctg aacagcttct
     1801 gcttcttgat gtgaaagagc tggagcagtc tatttccgct attggttcgg aaagcagccg
     1861 ggataaaagg agagttgcct atttgattca gctttacttg cttttattgg aaaaatactc
     1921 tctggaatcg gaaaatattg ttttaatgtt gaagggctac aggtttttca gcaataagga
     1981 tatagaggaa ctgcgtcagg gtcttgacag aaacgatata agagaggccc ttggacaggt
     2041 ttataagctg atgcggcatc tgaaaaaaat tattattgat aaaaatacaa gcgaagcttt
     2101 ggaaaacata tattataaac gtcatgttgc aataggtata ccctcaatgt acgggcagta
     2161 tattgaacca aaatttgagg ccctgggatt gatgttcagg ctggaaaagg ccgcatcaaa
     2221 gatgatggtg cagcttcttc aggatatcaa tctcgaatac atatctgcca agaccttcag
     2281 ggatgtatat gatgtattgg gattgttcaa ggaagggctt gaactggacg gcatatacaa
     2341 ccagggattt aattcaaact ttgaaatgtt caagtacagc cttacctctc caagcttttc
     2401 actggatcaa tacatagaca tatttcagtt catggcacag aatatcaagc aaataataaa
     2461 cgagtacttc cttgatgttt acgaaagccc gctgaaagtg gcaataccgc aaatatttga
     2521 tatcaagggc cctctttctg atcctaaaaa caagcagctc tatcatatgg aatcggagag
     2581 attcttcaga gaaattctgt catccgcctt tttggttcag gatctggata attttattac
     2641 caatctggta agcaccttaa gaagcatggt tgacaattat tcaggtgata tcattaagag
     2701 tatgatgaca tatgacccgg atctgactat aagtcccctt tatatggaga cggtggaaat
     2761 ggataatccg gtattccttg gagcaaaagc atatttcctt aaaaaaatga tatcttatga
     2821 ttttccgata ccttatgggt ttgttcttac aactgaggta ttcaggcata ggaggattat
     2881 cacggagcac ccgtatatgg aacaggagat tgatcagcta atacttaacc acatatggga
     2941 attggagaaa attacacggc agcagttcgg caatccaaaa aatcctctgt tgttttccgt
     3001 aaggtcaggt acggcaatct ccatgccggg cgctatgagg acttttctga atgtaggaat
     3061 gaatgacgaa attgccgaaa ccttcagcaa gaagccaaac cattcatgga ctgcctggga
     3121 ttgttacagg cgcttcattc aaagctgggg tatggcttac ggaattgaga gggatgtttt
     3181 tgacggagtg attcttgagc acaaagtaaa gtttggcgtt gagcagaaaa ttcaattcac
     3241 tgccgagcag atgaaagaaa tagcctatgc ctataagaag gtgcttggcg attacggaat
     3301 tcatattgaa aaagacccct tcaagcagct gaagcaggct atactaagcg ttattgattc
     3361 atggtcctcc ggcagggcaa tatactatag agagcatctg cagatcgcag acgaatgggg
     3421 gacagccgta atagtgcaaa aaatggctct cggcaaccta tcagagcgct ccggtacggg
     3481 agtagtattt acaaacagcc cgcttaaaac caagtccggt ataaatctgt acggagattt
     3541 tacattatgc agccaaggtg aggatatagt atcagggctg gtgcacacgc tgcctattac
     3601 cgaaagccag cgcagggatt attacagcga ctgcagcata tctctggagt ctgcgtttcc
     3661 cgatatatat aatgctttgc tggatttggc caaacagctt attgagacgt acggcttcat
     3721 gcaccaggag atagagttca cctttgagtc cgagaatcct gatgacctgt atatacttca
     3781 gaccaggaac cagaagctga agaaacagaa gcggctgtcc agcttcatac cggctcctga
     3841 tgaaatgaag cttgcagggc gggggatagg catcggcggc ggagcgttga cggggatatt
     3901 gacctttgac atggaggact taaaggaagc cgccaataaa catcctgaag agaaacgtat
     3961 actggtaagg cctgatactg tgcctgacga tatacctttg atattcagca gtgacgggct
     4021 tatcacaggc aagggggggg ccacatccca tgctgcagtt actgccgcaa gccttggaaa
     4081 ggtgtgcgtt gtgaactgca aaggtctgat agtaaatgaa gcagaaaaga gatgcagtat
     4141 aaatggtata agcttaaaat cgggagacag gatatccata gacggtaatc ttggaaatgt
     4201 atatgaaggt gtatacgaaa tacaatatga atagtataac gtattaattt aatgatatat
     4261 cacaataagc agcaataagg aaatataacg cataatttaa gatattgaga agaaatgtgc
     4321 tacatgatat aattagactg taaagctatt aagggatagg agtgattaaa tgaatgcacc
     4381 tttacaaagc aaaaggatgc tggggattaa tggtataggc cgtatcggga aactgacctt
     4441 atggaatcat ctcaacctca agcattttga cggaatagta atcaatgccg gccgtgaggt
     4501 gggtaagaaa gctgatgaca ttattcatta tctgactacc gattcaactt atggcaccgt
     4561 tgacagattc ctgtatggct atacgggcaa gtcctgcaat gtgaaagtat tggatgaaac
     4621 tgaatgtgta tttgaaatga acggaatgcc tgtcaggata cttagaaagg aaagaaatcc
     4681 aaggaatata gattggtcaa gagaaggggt cagactggtt gttgattgca cgggagtatt
     4741 tttagatccg acgcagccat cagattcccc caaaggaagc atcagaggcc atttggaagc
     4801 gggggcggag aaggtaatcg ccagcgcgcc tttcaaaatc aaggactctt cacaaaggat
     4861 gcctgatgac agcgctttgt ttgtatatgg tgtgaatcat gccagctatg atcctgccag
     4921 acatcacata ttgtcggctg caagctgtac tactacagga ttggcgcaca tgatcaagcc
     4981 tctcctagat acagaagaga cttcaagaat aataacggca tcaatgtcta ccgtacacgc
     5041 cgcaacaaac aatcagaaca tattggatgc tcctccgaag gcaggttcaa aggatctgag
     5101 gagaaacagg tcagtgttca ataatataat tccgacatca acaggtgctg ccatagcact
     5161 ggaagaaata cttcctgaaa tcaaaagggt agggtttatg gctgactctg taagggtacc
     5221 tacaagtaca gtgtcgctta tatctttgaa cattactttc cgaacggatc tcaatctgac
     5281 cggggagcct ataataaaca gagagtttat aaatgatatt tacagaaaag ctgcatccgg
     5341 cgcacaaaag ggcctattga tattcagcga agaacagaat gtatcttccg atttaatggg
     5401 atgccaggct gcggtaatta ttgaaggaag cgagaaccat accagaacag ggttcctgcc
     5461 tctttcttcg gaagtcctga gagaatacgg aatagataca gcgcaggata tcaatttacc
     5521 tgtaacccat gccaagatat tcggctggta tgacaacgag ttcggcaatt atgtcaattg
     5581 cctcggaaag ctgacggtat atgttgacaa gaatatgatt tagatttagg gatacactgt
     5641 tatgcttcgg tagaaatacc ggagcatttg tttttaaaaa tgaagtaaca atgccatgta
     5701 ccgcaacata tactataaga gtataacatc agtagagatg aactcaactg atgttatagc
     5761 ctccggcgga tgcgaacgat ttcgcaagga agattatttc acgacctgaa atacgaaatc
     5821 ggcgcgcaag tatgctggtg gcatacttga aatatgtgag gtgtatggca tttgattatt
     5881 catgttgtaa gagctgggga cagcttatat tcgatttcaa ggcgctatgg ggtaccggtg
     5941 gaaagaattg catccgacaa cgaattaaat gtttctgaaa gcctggtgat aggccagaca
     6001 attgttgtta tggaagggac aagacggcac accgtgaggg caggggagtc gctttattca
     6061 attgcgagac aatacggaac aactgttgcc ggaatacaag cggccaatca gattgccaac
     6121 ccggctatga tcagacccgg aatggtactg acaataccat caggaacaga caagctgggt
     6181 accattgatg taaacgggta cgcatttccg aatatcagta tggatgtatt gagaaaaaca
     6241 ttgccgtact taacgtacct gagtattttc agctatgaag tcaaacccga cgggagcttc
     6301 aggccgatta atgatgagcc tctgattgca gcagccaggg cagcaaatgt tgcacccctt
     6361 atggtgataa ccaatctgag ggagggcggc ggctttgaca gcgacttggc aaagacaata
     6421 ctgacaaatg aacaggttca ggatacactg attaataatg tgatagaaaa ccttagagca
     6481 aaaaactaca ccggacttga catagacttt gaatacgttt atccggaaaa cagggaagac
     6541 tacaataact tcctgagaaa ggtatcttcc gggctcagac cgttaggtta ctcaattact
     6601 acggctctgg cgcccaaaat agcggcggat cagcccgggc ttctttatga ggctcatgat
     6661 tatcctgtcc acggagcact ggttgaccat gtgatactta tgacatatga atggggctat
     6721 acctttgggc ctcctatggc tgtggcacct atcaacgagg tgcggaaggt cattaattat
     6781 gcaatgtcgg caatcccaag acgaaaaatt ttaatgggaa taccgaatta cggctatgat
     6841 tgggttctgc cctatgtgga aggcagcagg gcaacaacaa tatccaatgt tggagcggta
     6901 gaccttgcca gaagggaagg ggctgccata cagtatgatc aaaccgcaca ggctccattc
     6961 ttcaactatt atgatgataa cggaagacag catgttgtat ggtttgacga cgcccgcagc
     7021 atggaagcga gattaaggct ggtaaatcag tacaatctcg ggggcgtaag ctattggacc
     7081 atcggaaggt tcttcccgca gaactggttg gtcctaagct cattgtacga tgtaagaaaa
     7141 gtgctttagc atctcaaagg agacagtcaa tcaaggctgc ctccttctac tgcacattag
     7201 aagaataatg cgtcacggag acatggggac ggggaca
//
LOCUS       DAOOAB010000078         6752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_13391_length_6752_cov_13.791698, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000078 DAOOAB010000000
VERSION     DAOOAB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_6752_cov_13.791698"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1115)
                     /locus_tag="PKU88_11790"
     CDS             complement(159..1115)
                     /locus_tag="PKU88_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPX69991.1"
                     /translation="MKKYIVLTLAVVLVMSAALTGCAPQKTNLILATGGTSGTYYPFG
                     GSMAQIFNSKIPNMNVTAQATGASVENLKLISQKEAELAIVQNDMTDYAYNGTESFKD
                     SKVENVRVIANLYPEVIQIVSSADSGINTLTDIKGKKFSVGAPGSGVEANARQLLEVM
                     GMTYNDFSANYLSFAESADSLKDNHIDGFLFVSGVPNAAIQDTATTSSLNFVSIPDDV
                     INKLIEKYPFFTEYIIPAGTYKGQTGDVKTVAMKATLVAGAEVSEKVIYDLTKALFEN
                     QPELASAHAKGAELSLESAVDGVSVPFHPGAEKYFKEVGAIK"
     gene            complement(1239..2132)
                     /locus_tag="PKU88_11795"
     CDS             complement(1239..2132)
                     /locus_tag="PKU88_11795"
                     /inference="COORDINATES: protein motif:HMM:NF020248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX69992.1"
                     /translation="MTSFIVIDDDRTVRSVLIKIIEQYSLGEVYAEADNGLLGEEMIL
                     RYKPDIVIIDFLLPHQDGMSIIKKVKSLNVKSMFIMLSQVSDKDMIARAYEMGIEFFI
                     SKPINVIEVVNVIRRVREYLAMKKTFEAIESTVMNLGRSADSEPSRKSGVVKRLLNQI
                     MADLGILGDLGSRDIMNICTMLYENPEYGDTLEETQLSDLLKLLQSKYTSEGRVSKSD
                     FKAIEMRMRRTVAKAMKNIAAMGIEDFSNDKFVLYSSSLFDYVEVKAEMDYIRGKAMY
                     NGKVNIKSFIKRLLVMLEQIA"
     gene            complement(2125..3420)
                     /locus_tag="PKU88_11800"
     CDS             complement(2125..3420)
                     /locus_tag="PKU88_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX69993.1"
                     /translation="MRNKYKEMLIVGMCTALMGQVNFYPFGTDFRISIGVVAFTFLML
                     YFHTIPIISSSIITGLSVLMLRVGIDVFANSMAMDATFYKHMPGMMYYITYGIIIEGS
                     SFRKLFEKPIYFIMVLITADILSNIFELLIRNQLNANSFDTIFSTIMLAAVIRSCLVL
                     ALFWIIKYYSLFVTKEEHQRRYKELLLLTSKLKSEIFFLKKSMQDIENAMAKSYSIYN
                     TIKESNPMGKEDIEIITADCLNLSIDIHEIKKDYNRIVISMEKLMPAEDTHKTMSISE
                     VFETIGDIFNRYIEVINKDITLSFEIEKDYKIKEYFIIMSILNNLIQNSVEACFRSDS
                     SIKVGCFIHESMLVFAVSDNGKGIGKKERELIFEPGFTTKFNPETGHVSTGLGLTHIK
                     ILLEHLEGKININEDIEGITKFVIEIPINKIVGEVEDID"
     gene            3650..4609
                     /locus_tag="PKU88_11805"
     CDS             3650..4609
                     /locus_tag="PKU88_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPX69994.1"
                     /translation="MKNKVINLALTIISVSSLLFILTTSVFADFGDSTLNKGITHPDV
                     HVLQEKLNTLGYFNNGQFTDYFGDKTYNALIAFQRDEGLNPDGIAGNETFKRLSLKIT
                     QAEILPKDFVPIKEGDIGDKVVDIQKKLRDLNIYKTEINSFYDQTTIEAVISFQAANA
                     LPRTGIVDSATIIKLNTAYNSILADRASASRALLNSKVVEYAKQFLGKPYVWGASSGK
                     AFDCSGFTVYIMKKFNVSLPRTASGQINSGTKVGKDDLQAGDLVFFTTYKKGPSHVGI
                     YIGGNKFIHASSGVDKVTITDLDSSYYKSRYLGARRYSLTDTK"
     gene            4765..5859
                     /locus_tag="PKU88_11810"
     CDS             4765..5859
                     /locus_tag="PKU88_11810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPX69995.1"
                     /translation="MSINSTSKSKQKELIKKLRSSNILLKRYSMITDSIMKITSEVFS
                     SGEIDYILQTIMDKAIEIIPGAQRGSILIYNGSVLEFKASRGYDSSALENLKLSIEEV
                     FQYNSEDFFEPCIVSNQERFNREHLPDYKYEILQKGLGINPKSTLSCAFQVDNKFQGI
                     INLDNMDDNTAFRKEDKPLIKHLAVQIGIALKNARQIEKILFLSRHDSLTGIYNRCYF
                     EELLMHIMQTSKASASVFSLAMLDINNLKYINDTYGHEAGDLMLKKFAQCVNEIITGD
                     DIFARYGGDEFAIIFKDNDNIQAANKINNIRQTLLNLPFTYCGKTIAPIGFGCGIVQF
                     PADGSEFQDFMRLADISMYQDKKKSKKPVI"
     gene            complement(5883..6596)
                     /locus_tag="PKU88_11815"
     CDS             complement(5883..6596)
                     /locus_tag="PKU88_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX69996.1"
                     /translation="MNIIEYLKQQKVIPQYKADKFIELLATYEENDVLYENAIKQLDL
                     TNDEYDRLFTELEKGNYVDRIYIIVCPYCDSLGNTYVQSFDIPETDKCRFCHNVFNHK
                     ENNMEAFRLYFNYHKNIDILTELSQKINYNQERIFEIAEEIKELETKRKSLQDENIKF
                     VTLRNELQYDKSESKAYEKTVDKYKEDKVKYIGTLIYIKKCVAKCSKCNEAFELFNVN
                     GKYIGVCPSCDFEIELLMK"
BASE COUNT         2050 a         1406 c         1156 g         2140 t
ORIGIN      
        1 acaattttac cggtcctgcc gttggaaatt gtaaacctgt ttaataccgg tacatttaat
       61 aaaataatta tcagcaaaag taaaaaaact gcatatataa actttttcat aaatgaagaa
      121 gccgggccgg atatttccag cccggcagcc ctcccaaatt atttaatagc tccaacctct
      181 ttaaagtatt tctctgctcc cggatggaac ggaaccgata cgccgtcaac agcgctttca
      241 aggcttagtt ctgctccctt tgcatgggca gaggctaatt ccggctgatt ctcaaataat
      301 gcctttgtca ggtcatatat taccttttcg gaaacttctg caccggcaac caaagttgct
      361 ttcattgcta ctgtctttac atccccagtc tggcccttgt aggttccggc tggaataata
      421 tattcagtaa agaaaggata cttttcaatc agtttattga ttacatcgtc cggaattgaa
      481 acaaagttca ggctgcttgt agtagctgta tcctgtattg ccgcattagg tacgccggat
      541 acaaacaaga aaccgtcaat atgattgtct ttcagagagt ctgctgattc agcgaaagac
      601 aggtaattgg ctgaaaaatc attgtatgtc atacccataa cttccaacaa ctgtcttgca
      661 tttgcttcaa ctccgcttcc cggagcaccc actgagaact tcttcccttt aatgtctgta
      721 agagtattga ttccactgtc tgcggaagaa acaatctgaa ttacctccgg ataaaggtta
      781 gcaataaccc ttacgttttc aaccttgcta tccttgaaag actctgttcc gttataagcg
      841 taatctgtca tgtcgttctg gactatcgcc agctctgctt ccttctggct gataagcttg
      901 aggttctcaa ccgatgcacc tgttgcctga gctgttacgt tcatgttcgg aatctttgag
      961 ttgaatatct gtgccattga accgccaaac ggataataag taccggatgt accgccggta
     1021 gccaatatca aattggtctt ctgcggggca cagcctgtaa gtgctgctga cataacgagc
     1081 accacagcca atgtcagtac tatgtacttt ttcatagaat ctcctcctcc aatttattat
     1141 tattagttta ttacaacagt aacaaatagt aacaaaaaaa aacgtgaatt tacatccacg
     1201 ttgagaaaat ttagaaaatt gattttgaat aattatgttt atgcaatttg ttccagcatc
     1261 accagcagtc tttttataaa ggactttata ttgactttgc cgttatacat tgctttgccc
     1321 ctgatataat ccatttccgc ctttacctca acataatcga acaaggacga actgtagaga
     1381 acaaacttgt cgttactgaa gtcctcaatc cccatagctg caatattctt catcgctttg
     1441 gccaccgtcc tcctcattct catttcaatt gccttaaagt cactcttaga aaccctgcct
     1501 tccgaagtat acttgctctg cagcagtttt aaaagatcgc tcagctgagt ttcctccaag
     1561 gtatccccat attcagggtt ttcgtacaac atggtgcata tgttcattat atccctgctg
     1621 ccaaggtcgc ctaatatccc cagatcagcc attatctgat tcagcagcct tttaacaact
     1681 cccgattttc tggaaggctc ggaatccgcg ctcctgccca ggttcataac ggttgactca
     1741 atggcttcaa aggttttctt cattgcaagg tattccctga ctcttcttat aacattgaca
     1801 acttcaataa cattaatagg cttgctgata aaaaattcaa tccccatctc ataggctctt
     1861 gctatcatat ccttgtcact gacctgtgac agcattataa acatactttt tacattcagg
     1921 gatttgacct tttttattat agacattcca tcctgatggg gcagcagaaa gtctattatt
     1981 acaatatcag gcttataccg gagtatcatc tcttctccca gcaatccgtt atctgcttct
     2041 gcatatactt ctccaaggct gtactgttcg attatcttta tcagcacgct tctgaccgtt
     2101 ctgtcatcgt caataactat aaagctagtc aatgtcttcc acctcaccaa ctattttgtt
     2161 tatcggtatc tcgataacaa attttgtaat accctcaata tcctcattaa tatttatttt
     2221 cccctccagg tgttccagca atatctttat atgtgtcagg ccaagtcctg tcgatacatg
     2281 ccccgtctcc ggattgaact ttgtagtaaa gcccggttca aaaataagct cacgctcctt
     2341 tttgcctatc ccctttccat tgtcactgac tgcaaaaacc aacatgcttt catgtatgaa
     2401 gcacccgact tttatagagg aatcgcttct gaaacaagct tcaacagagt tctgtatcaa
     2461 attgttcagt attgacatta ttataaaata ttccttgatt ttataatctt tctctatctc
     2521 aaagctgaga gtgatatcct tgttgattac ttcaatatat ctgttaaata tatcacctat
     2581 cgtctcaaat acctcactta tactcattgt cttgtgagta tcttcagccg gcataagctt
     2641 ctccatgctt ataactattc tgttatagtc cttttttatt tcatgtatgt caatagacaa
     2701 attaagacaa tcagcagtta ttatttctat atcttcctta cccataggat tgctttcctt
     2761 aatagtattg tatatagaat aactctttgc catagcattc tcaatatctt gcatagactt
     2821 cttaagaaag aagatttcag actttagttt tgaagtcaat agaagaagtt ctttgtacct
     2881 tctctgatgc tcttctttag tcacaaacag gctgtagtat tttattatcc agaacaaagc
     2941 caataccagg cagcttctga ttacagcagc cagcatgata gtagagaaaa ttgtgtcaaa
     3001 tgaattagca ttaagctgat tccgtatcag cagctcaaaa atgtttgaca gtatatctgc
     3061 cgttatcaat accataataa aataaatcgg cttttcgaag agcttcctga aactcgatcc
     3121 ttcaataatt atgccatatg taatataata catcattccg ggcatatgct tataaaatgt
     3181 tgcatccatg gccattgagt tggcaaatac atctattccg actctgagca taagaactga
     3241 taatcctgtt ataatcgatg aagaaataat cggaatggta tgaaaataaa gcatgagaaa
     3301 agtgaatgca acaaccccta tgcttattct gaaatccgta ccaaagggat agaaattcac
     3361 ttgccccatg agcgcagtgc acatacctac tataagcatt tctttatatt tgtttctcaa
     3421 agcagcaacc tcattacctg atttcaattt ttttatatat ttaactctga atatgcatga
     3481 ttttattgta attatatcat attatggtat ggggaacaaa atatgtgtat ttcttcctct
     3541 gctgctgaac tgcggaaata tttcttttat atttatttcc ccgtaataat actgtataat
     3601 attatttaat gtactgtaaa taagtacatt tccaaaggag gctgaaacat tgaaaaataa
     3661 agtaataaac ctcgcactaa ctattatatc agtgtcctcc cttctcttca ttcttacaac
     3721 atcggtattt gcggattttg gagacagcac cctcaataaa ggcattaccc atcccgatgt
     3781 ccatgtcctt caggaaaaac tgaatacact cggctacttt aataacgggc agttcactga
     3841 ttatttcggg gacaagacat ataatgcact gatagctttt cagagggatg aaggactgaa
     3901 tccggatggc attgcaggca atgagacatt taagcgcctc agcctgaaaa tcacacaggc
     3961 ggaaatactt cctaaagact ttgtgccaat aaaagaaggc gacataggtg ataaggttgt
     4021 tgatatacaa aaaaagctga gggatctgaa tatatataaa actgaaataa actcctttta
     4081 tgaccagact accatagaag ccgtaataag cttccaggca gcaaacgcac ttccccggac
     4141 cggtattgtc gacagtgcga caataatcaa gcttaatact gcttacaaca gcattcttgc
     4201 cgacagggca agtgcaagcc gcgctctgtt gaattcaaag gtagttgaat atgcaaagca
     4261 attcctgggt aagccttatg tatggggggc ttcttccggc aaagcttttg actgctcagg
     4321 cttcacagta tatattatga agaaattcaa tgtcagcttg cctcgtaccg cttccggcca
     4381 gatcaacagc ggaaccaaag tcggaaaaga cgatcttcag gcaggagatt tggttttctt
     4441 cactacatat aaaaaaggcc ccagccatgt gggaatatac ataggcggga acaagttcat
     4501 acatgcaagc tcaggagttg ataaggtcac cattaccgat cttgattcca gctactataa
     4561 gagtagatac ttgggtgcaa gaaggtattc attgacagac accaaataga aacagcttcg
     4621 catggctgtt tctatttcat tttgcatatt attcgataaa cttgtatatc caatttattc
     4681 ttttacttag tgcttttttt ggtattatac aggtagaaga catattttga tatttttctt
     4741 aattaaagta tttggggtga agaaatgagt attaactcta catccaagag taaacagaaa
     4801 gaactgataa aaaagctcag aagcagcaat atacttctca agaggtacag catgattaca
     4861 gattccatta tgaaaataac aagtgaagta ttttcctccg gcgaaattga ttatatactt
     4921 caaacaataa tggataaggc aattgagata ataccaggtg ctcagagagg cagtatactg
     4981 atatataacg gcagcgtttt ggaatttaag gcttcccggg gatatgattc cagtgcgctg
     5041 gaaaatctga agcttagtat cgaagaggta tttcaatata attcggaaga cttttttgaa
     5101 ccttgcatag taagcaatca ggaacgcttc aatagggaac atcttccgga ttataaatac
     5161 gaaatattgc aaaagggttt gggcattaat ccaaaatcga ctctcagctg cgcatttcag
     5221 gtagacaata aatttcaggg aatcataaac ctggataata tggatgataa tacggctttc
     5281 agaaaagaag acaaaccatt gattaagcac ttggcggttc agataggaat tgcccttaaa
     5341 aacgccagac aaatagagaa aatcctattt ctttcaagac atgacagttt gacaggtata
     5401 tacaaccgct gctactttga ggagcttctg atgcatatca tgcaaaccag caaggcttcc
     5461 gcctcggtct tttccctggc aatgctggat ataaataatc ttaagtatat taatgacacc
     5521 tatggtcatg aagccggtga cttaatgctc aaaaaattcg cccagtgtgt aaatgaaatc
     5581 ataacgggcg atgatatatt cgccagatat ggcggagatg aatttgcaat tatctttaaa
     5641 gataatgata acattcaagc agcaaataag attaacaaca taaggcaaac tttattgaat
     5701 ttgcccttta catactgcgg taaaactatt gccccgatag gcttcggatg cggaattgtg
     5761 caatttcctg cagatggatc agaatttcaa gattttatga ggcttgcaga tataagtatg
     5821 taccaagata aaaagaaatc aaaaaaacct gttatatagc aggttttttg atttttgatg
     5881 atttatttca ttaataattc tatttcaaaa tcacagctgg gacatacacc tatatacttt
     5941 ccattgacat tgaacagttc aaaagcttca ttgcatttac tgcacttcgc cacacacttt
     6001 tttatataaa taagggtgcc gatatatttg accttatcct ctttatattt atcaactgtc
     6061 ttttcatacg ccttgctttc gcttttatcg tactgcaatt cattgcgaag cgttacaaac
     6121 tttatatttt cgtcttgcag ggatttcctt tttgtttcca attctttaat ctcttcagct
     6181 atttcaaaaa tcctttcctg attgtagtta attttctggc tgagttctgt caaaatgtct
     6241 atattcttat gataattgaa gtataagcga aaagcttcca tattgttttc tttatgattg
     6301 aaaacattat ggcagaacct gcacttatca gtttcgggta tatcaaagct ttgaacatat
     6361 gtattcccca agctgtcaca atatggacaa acaattatat atatcctgtc tacatagttc
     6421 cctttttcca attcggtaaa aagcctgtca tattcatcat ttgtgagatc aagctgtttt
     6481 attgcattct cataaagcac atcattctct tcgtaagtag ccagaagctc gatgaatttg
     6541 tcggctttat actgcggaat caccttttgc tgcttaaggt actcaataat gttcatttta
     6601 ttccctcccc gctatgatat aattactcgc ccccattcaa aaccaagcac actgattatc
     6661 ttattctata aataatcaaa atatcctttt attaaaaact gattattcaa aaaatatgaa
     6721 gaatgcgacg gggacacggg gacacgggga cg
//
LOCUS       DAOOAB010000079         6704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_13502_length_6704_cov_15.442021, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000079 DAOOAB010000000
VERSION     DAOOAB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13502_length_6704_cov_15.442021"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..900)
                     /locus_tag="PKU88_11820"
     CDS             complement(31..900)
                     /locus_tag="PKU88_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPX69997.1"
                     /translation="MSENINLMKVMQTKFQKLSKGQKIIAQYIMKNYDKAAFMTAAKL
                     GEVVEVSESTVVRFAITVGYKGYPQLQKQLQEMVRTKLTTVQRIEMSSDYTNQASVLK
                     TILKSDIENIRGTMEEIDSSAFEQAVRAIIEAKSIYIIGLRSSNTLSEFLGFYLNFVR
                     DNVHVVTYTIGDIFEQLFRIGKDDLVIGITFPRYSTRTLKAFEYAKSRGANTIAITDS
                     ILSPLCANADYTLIAKSNMESFVDSIVAPMSLLNALIVAVGIKQKHNISDSFAKLEDI
                     WNKYNIFSADKDE"
     gene            complement(916..1452)
                     /locus_tag="PKU88_11825"
     CDS             complement(916..1452)
                     /locus_tag="PKU88_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPX69998.1"
                     /translation="MERYEIRLSGSGGQGLLLGGIILAEGAINDGKNSVQTQSYGPEA
                     RGGASKSEVIISSEEIDFPKVRNCDILLSLTQKAYDEYNEGLKEDGILIVDSSVNAVK
                     SGNIKIYSIPIIDSAVKDLGKPMVTNIVALGVLVEITKVISKESLEKAVLERVPKGTE
                     ELNRKALAKGYEIANKLM"
     gene            complement(1452..2276)
                     /locus_tag="PKU88_11830"
     CDS             complement(1452..2276)
                     /locus_tag="PKU88_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPX69999.1"
                     /translation="MPSVLVENYFRTEKLPQIWCPGCGHGIITRSIASAIEKSGIDKN
                     KICIVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGIKMAKPEIKVIVIMGDGDATAI
                     GGNHLIHACRRNIGITAIVYNNSIYGMTGGQYSPTTPKYDKGTTAPYGNIDKAFDICK
                     LAASAGATYVGRATAYHASLVTSLVQNAINNEGFSLIEAISMCPTYYGRKNKKGSAVS
                     MMEWQKNSAINISAAAKMSPEALEGKFLIGELKNSPEPEYTNEYQKIIDRLKKESQ"
     gene            complement(2278..3411)
                     /locus_tag="PKU88_11835"
     CDS             complement(2278..3411)
                     /locus_tag="PKU88_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPX70000.1"
                     /translation="MKSKVRLMQGNEACVEGAIAAGMRFYAGYPITPSTEIAELSAER
                     LPLVGGRFMQMEDEIGGMAAIIGASLTGAKSMTATSGPGFSLKQENIGYASIAEVPCV
                     IVNVQRGGPSTGMPTSPAQGEIMQARWGTHGDHPVIALTPSSVRETFDLTIKAFNFAE
                     KYRTPVILLMDEVIGHMREKVQLPDPSEIVIIDRKRPDCSSEEYLPYEADNDLVPPMA
                     DFGTGYRYHVTGLVHDETGFPSNSTAVADKLIRRLSNKIEIHRDEIESWEEENTEDAE
                     VLILSYGCVARSSKSAMNILRGRGIKVGTFRPITIWPFPEKRLRELAAKVKTIVVPEM
                     NLGQLVLEVERICGCNAKIEKLNKVSCEIIYPEEIVSKIEEVL"
     gene            complement(3395..3628)
                     /locus_tag="PKU88_11840"
     CDS             complement(3395..3628)
                     /locus_tag="PKU88_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX70001.1"
                     /translation="MEDIKAGEIQNEKVVINPAWCKGCGICVAFCPVKALELKNDKVV
                     MVDVDKCTQCGMCELRCPDYAIYLRRKKNEKQG"
     gene            complement(3777..4565)
                     /gene="surE"
                     /locus_tag="PKU88_11845"
     CDS             complement(3777..4565)
                     /gene="surE"
                     /locus_tag="PKU88_11845"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPX70002.1"
                     /translation="MLNILISNDDGIYSKGLYELAKRMAALGKVTVVAPDKEQSAIGH
                     AITMHQPLRCGEIKLYDLDINAWWVNGTPADCVKLGVETILSVQPDLIISGINDGENL
                     GTDIIYSGTVSAAIEGSMFNIPSIALSYASRCAADFSAPAEVAAQVIQQVLEHKGKNN
                     MLLNVNIPEIESIELLKGVKVTRLGVKKYRNNFEERKDPRGNSYYWLAGELVQDEIGE
                     DTDIYAVRNGYVSITPIHIDLSGYEEIKKISSWNISLNKNLTII"
     gene            4644..5282
                     /locus_tag="PKU88_11850"
     CDS             4644..5282
                     /locus_tag="PKU88_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70003.1"
                     /translation="MIQIDDAGSGSLVGGTCIGILRKETGEYYYEILPVQLFSETNFK
                     NKSYIYYTTKVVMNAFEKLSVKKEERINICQGYMFEDTRKFLARSGYTFTNTKIDEPL
                     QSLIEKTFENYTISLGLPMGFIAYTKYPFHFHRLLKWVYADFDNRTELCKTGWKSWQK
                     YSNAKVESYYDKLVKSSYICLKCGNRIPDNSNVKVIKFYSDRYNYVYVHKNC"
     assembly_gap    5372..5471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5514..>6704)
                     /locus_tag="PKU88_11855"
     CDS             complement(5514..>6704)
                     /locus_tag="PKU88_11855"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPX70004.1"
                     /translation="KSKLKNTKLQMLLRGQNILGYKNYADDVVEEFVRLSIKNGIDII
                     RIFDALNDLRNIETAVKAAKKYGVQAQGTVVYTTSPVHDIDLYVELAKKVQEMGCDSL
                     CIKDMSGILAPYYGYELIKALKKHISIPIQLHTHFTSGMTDMTYLKAIEAGVDIIDTA
                     ISPFGLGTSQPPTEPMVATLKGTEYDTGLDLDLLCEIAEYFRKIKDKYLANGILSPNV
                     FNIDTNVLSYQIPGGMLSNLIAQLKQTNNMDKYNDVLKEVPRVREDLGYPPLVTPSSQ
                     FVGTQAVFNVISGSRYKMIPNEIKDYVSGRYGRPSVPISEEIKKIIIGDEEVITCRPA
                     DLIEPQLESLKQEIKEYYEQEEDVLSYAVFPQVAMKFFKDRQAKKYGIDSSLADKDNK
                     TYPI"
BASE COUNT         1669 a         1571 c         1192 g         2172 t
ORIGIN      
        1 aatcctgctt tgtacaaagc aggattttta ttactcatcc ttatccgccg aaaaaatatt
       61 gtatttgttc catatatcct ccagctttgc aaaggaatcg gatatattat gcttctgctt
      121 gattcctacc gctacaatca gtgcattcag caagctcata ggagcaacta ttgagtccac
      181 aaaagactcc atattgcttt ttgcaataag ggtataatct gcattagcgc acagaggcga
      241 aagtatgctg tctgttattg ctattgtgtt tgctcctctg gattttgcat attcaaaggc
      301 ctttaatgtt cttgtagaat accttgggaa tgtaattcca attaccagat catctttgcc
      361 gattctgaaa agctgttcaa aaatatctcc gatagtatat gtcacaacat gaacattgtc
      421 ccttacaaaa ttgagataaa aacccagaaa ctctgaaagt gtattggaac tcctcaagcc
      481 tattatgtaa atgctttttg cttcaattat ggctcttaca gcttgttcaa aggctgagct
      541 gtctatctct tccattgttc cccttatgtt ttcgatatca gacttgagga tagtctttaa
      601 cacacttgcc tgattggtat aatccgaact catttcaatt ctttgaactg ttgtaagctt
      661 ggttcttacc atttcctgca gttgcttctg caattgggga tatcctttat atccgactgt
      721 aatcgcaaac cttacaaccg tcgattcact tacttccacc acttccccaa gcttggcggc
      781 agtcataaat gcagccttat cataattttt cattatgtat tgtgcaatta tcttctgccc
      841 tttactcagc ttttgaaatt ttgtctgcat gactttcatc aagttgatat tttcactcat
      901 actgcctatc tccatttaca tcagtttatt tgcaatttca tatccttttg caagtgcttt
      961 cctgttgagt tcctccgtac ctttcggaac cctttcaaga acagcttttt ccaaggattc
     1021 ctttgatata acctttgtta tctcaaccaa tactccaagg gcaacaatat tggttaccat
     1081 cggcttgccc agatctttaa cggctgaatc aattatagga atgctgtata tcttaatatt
     1141 ccctgatttt acagcattta cggagctgtc tactataagt atcccatctt ccttgaggcc
     1201 ttcgttgtat tcatcatagg ctttttgcgt caatgacaac agtatatcac aatttcttac
     1261 ttttggaaaa tctatctctt cacttgatat tataacctct gatttgcttg ctccgcctct
     1321 tgcctccggg ccgtaggatt gtgtctgtac cgaattctta ccgtcattta ttgcaccctc
     1381 cgccagaata attcccccca gaagcagtcc ctgccctcct gagccgctta gtctgatttc
     1441 atatctttcc atcactgact ctcctttttc aatctatcga ttatcttttg atactcattg
     1501 gtgtattccg gttccggact gttctttaac tcaccaatga ggaatttgcc ttccaatgct
     1561 tcaggagaca tctttgccgc agccgatatg ttgattgcac tgttcttctg ccattccatc
     1621 atacttactg cactgccctt tttattcttt ctgccgtagt aagtcgggca catgcttata
     1681 gcttctatca gggaaaaccc ctcgttgttt atagcattct gcaccaagga tgtgaccagg
     1741 cttgcatggt aagctgtggc tcttcctaca taagtggctc ccgctgaggc ggccagcttg
     1801 catatgtcaa aggctttatc aatatttccg taaggagctg tagttccttt atcatatttc
     1861 ggtgtggtag gcgaatactg gcctcctgtc ataccgtata tactgttgtt gtacactata
     1921 gctgttattc ctatatttct tctgcatgcg tgaataagat gattgcctcc gatagctgtc
     1981 gcatctccgt ctcccattat tacaataacc ttgatttccg gctttgccat ttttatacct
     2041 gttgcaaaag ccaatgctcg tccgtgagtg gtatgaagcg tattaaagtc aagatatccc
     2101 ggtgctcttg atgaacagcc tatgcctgac acaatacaga ttttgttttt atcaatacct
     2161 gacttctcta ttgcggaagc tattgatctt gttattatcc catgaccgca gccggggcac
     2221 catatctgcg gaagcttttc tgttctaaag taattctcaa ctaatacact gggcatatta
     2281 gagtacctcc tcaatcttac tgactatttc ctcgggataa atgatctcgc agctgacctt
     2341 gttcaatttt tcaatttttg cattacagcc gcatattctt tcaacttcca ggaccaactg
     2401 tcctaaattc atttcaggta ctacaatggt ttttaccttg gctgccagtt ctctcaaccg
     2461 cttttcaggg aaaggccaga tagtgattgg cctgaaagtg cctactttta tgcctcttcc
     2521 ccggagaata ttcatcgctg attttgatga cctggctacg cagccgtagg ataatatgag
     2581 cacttcagca tcttcggtgt tttcttcctc ccatgactcg atttcatctc tatgtatttc
     2641 tattttattt gaaagccttc ttatgagctt atcggctact gcagtgctgt tgctgggaaa
     2701 acctgtttca tcatgcacca atccggttac atgatatctg tagccggtac cgaagtccgc
     2761 catcggcggc accaggtcgt tatcagcttc atacggcaag tattcctcgc tgctgcagtc
     2821 cggcctcttt ctatctatga ttactatttc tgaagggtca ggtaattgaa ctttttctcg
     2881 catatgtccg attacttcat ccataagaag tataaccggc gtcctgtatt tttctgcaaa
     2941 attaaaagcc tttattgtaa ggtcaaaggt ttctctgact gaggaaggag taagggcaat
     3001 aacaggatga tccccatgag taccccaacg ggcctgcatg atttcgccct gagccgggga
     3061 agtaggcatt cccgtactgg gcccccctct ctggacattt actattacac aaggtacttc
     3121 agctattgaa gcataaccta tgttttcctg cttaagagaa aaaccggggc cgctggtcgc
     3181 cgtcatgctt ttggctcccg taagggaagc tcctattatg gcggccattc ctcctatttc
     3241 gtcctccatc tgcataaatc ttccgccgac caaaggcagc ctttctgcgg ataattcagc
     3301 tatttcagtt gaaggtgtaa tgggataacc ggcataaaac cgcattccgg cagcaattgc
     3361 accttcgacg catgcttcat ttccctgcat caatctaacc ttgcttttca ttttttttcc
     3421 tcctcagata tattgcatag tcaggacatc taagctcaca cattccgcac tgtgtacact
     3481 tatcaacatc gaccattaca accttatcat tcttaagctc caatgctttt accgggcaaa
     3541 aagcgacaca gatgccgcag cctttacacc aggcaggatt gattactact ttttcatttt
     3601 gtatttctcc tgctttgata tcctccattt tcattttttt gacctccaaa aattcattag
     3661 ataatgcagc ttttatttca tttattgtat ttacattggg aattctgcaa ctttcccttc
     3721 aatttttagt atatattata atattctcat ttgcaataat taatgccaaa gaaatttcat
     3781 attattgtca ggtttttatt cagtgagata ttccaactgc tgatcttctt tatttcttca
     3841 tagcctgaaa ggtcgatatg tataggtgtt attgacacgt acccgtttct taccgcgtat
     3901 atatccgtat cctcaccgat ttcatcctgt acaagttcac cggccagcca ataatagctg
     3961 tttccgcggg gatccttcct ttcctcgaaa ttattcctgt actttttaac tccaagcctg
     4021 gttaccttta cacccttcag taattcaata ctttcaattt caggaatatt aacatttaac
     4081 agcatattgt tttttccctt gtgttccaga acttgctgta tcacttgggc agcaacttcc
     4141 gcaggcgcgg aaaaatcggc tgcgcagcgg cttgcatagg ataaagctat tgagggtatg
     4201 ttgaacattg aaccttcaat ggctgcagat accgttcccg aatatattat gtccgtacca
     4261 aggttttcac cgtcattaat acctgaaatt atcaaatcag gttgtacgga caatattgtc
     4321 tcaactccca gcttgacaca atccgcaggt gtgccgttta cccaccatgc attaatatcc
     4381 aggtcataca gctttatttc cccgcatctc agcggttggt gcatggtaat tgcatggcct
     4441 atggcgcttt gctccttatc aggggcaaca acagtgacct tgcccaatgc tgccattctc
     4501 tttgccagtt catataatcc ctttgaatat attccatcat cgttggatat tagtatgttt
     4561 aacatttgta ccctcccact tcatgtttaa taattttttc ttttacatat actaaagcca
     4621 ctttgtactg tgcaggtgat aaaatgatac agatagatga tgcgggaagc ggcagcctgg
     4681 taggcggcac ctgcatcggc atcttgcgaa aggaaacagg agagtactat tacgaaatac
     4741 tccccgttca attattcagc gaaaccaact ttaaaaacaa gtcatacata tattatacaa
     4801 caaaagttgt aatgaatgct tttgaaaagc tctcggtaaa aaaggaagag agaataaata
     4861 tatgccaggg ctatatgttt gaagacacaa gaaaatttct tgcccggtca ggctatacct
     4921 tcacaaacac caaaatagat gaaccacttc aatcacttat agagaagacc tttgaaaact
     4981 atacaatttc cctcggcctt ccaatgggtt ttatagctta tacaaagtac ccctttcatt
     5041 ttcacagatt actgaaatgg gtatatgctg actttgataa ccgtacggaa ctttgtaaaa
     5101 caggatggaa aagctggcag aaatatagca atgccaaggt ggaatcatac tatgacaaat
     5161 tggttaaaag cagctatata tgcctgaaat gcggcaatag aatacctgat aattcaaatg
     5221 taaaggtcat aaaattctat tccgataggt acaattatgt atatgtgcat aagaattgct
     5281 gaagggggga gtgagacacg gggagacacg gggagagaca cggggacgtt tcacgtgtcc
     5341 ctccaggaca ggcgaaacgt ccccgtgtct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn naacgtcccc gtgtctcccc atgtctcccc gtgtctactt atattaaatt
     5521 ggataggtct tattatcttt atccgcaagg ctgctgtcta taccgtattt ctttgcctgc
     5581 ctgtccttaa agaatttcat ggcgacctgc gggaaaacgg catagctcaa aacatcctcc
     5641 tcctgctcgt aatactcctt aatttcctgc ttcagggatt ccaactgcgg ctcaattaaa
     5701 tccgcaggcc tgcaggttat aacttcctcg tcccctatta ttatcttctt tatttcctcg
     5761 gaaatcggga cgcttggcct tccatacctg ccgcttacat aatcctttat ttcatttggt
     5821 atcattttat acctgctgcc cgaaataaca ttaaagactg cctgggtgcc tacaaactgg
     5881 ctggaaggcg ttacaagagg aggatagcca agatcctccc tcacccttgg cacctctttc
     5941 agcacatcat tatatttatc catgttattt gtctgcttca actgggctat caagttggac
     6001 agcatccctc ccggtatctg gtatgacaac acattggtgt caatattgaa tacattgggg
     6061 ctcagtatcc cattggccaa atacttatct tttatctttc tgaagtactc cgcaatttcg
     6121 cacagtaaat ccagatccag gccggtatcg tattccgttc ccttcagcgt tgccaccatt
     6181 ggttccgttg gcggctgtga ggttcccagc ccaaaaggag atatggctgt atctattata
     6241 tctactcctg cctctatagc ctttaaatag gtcatatccg tcattccgct ggtaaagtgc
     6301 gtatgaagct gtatcggtat gcttatgtgc tttttcagcg ctttaataag ctcatatccg
     6361 taatagggag caagtatacc ggacatatcc ttgatgcaaa gggaatcaca ccccatttcc
     6421 tggacttttt tcgcaagctc cacatacaag tctatatcat gtaccgggct tgtagtgtat
     6481 acaactgtgc cctgggcctg gacgccgtac ttttttgctg ctttaaccgc tgtttcaata
     6541 tttctcaaat cattcagggc atcaaatatt cttataatgt ctatgccgtt cttgattgaa
     6601 agtcttacga attcttcaac cacatcgtcg gcatagttct tatatcccag tatattctgt
     6661 cctctgagaa gcatttgcag ctttgtattc ttaagctttg actt
//
LOCUS       DAOOAB010000080         6335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_14417_length_6335_cov_15.575796, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000080 DAOOAB010000000
VERSION     DAOOAB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14417_length_6335_cov_15.575796"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..703)
                     /locus_tag="PKU88_11860"
     CDS             complement(35..703)
                     /locus_tag="PKU88_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPX70005.1"
                     /translation="MLLIIAALLLFIFALRTISINTLHYYDPIYKGMEDDKKIAFACN
                     VVWGEEYLPQMLKLLEEKDIEITFFIGGQWAEKNEELLRNMHKAGHELGNHGYKHLYH
                     SKLTPESNKQEISKTEEIIKRITGYKTVLFAPPYGDINGTVVSSAEALGYKVIMWSID
                     TIDWNTRDYKKILQRVENKHHKGAIVLMHPTKVTVAALPQLIENLASHGYEITTVSDV
                     LEND"
     gene            complement(1025..1456)
                     /locus_tag="PKU88_11865"
     CDS             complement(1025..1456)
                     /locus_tag="PKU88_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX70006.1"
                     /translation="MKGTIYAIAAGIFIAMQQVFNARLGEKAGLWETNTFVHGSGFVF
                     SLIILMIAGNGSFAKIGETNRLYLLGGVLGVLIVFSAMIAVSALGATYSIAILLVTQL
                     IAATVIDSFGLFGSPVIEFDITKLLGIAIMIAGIIVFKLKG"
     gene            complement(1473..2204)
                     /locus_tag="PKU88_11870"
     CDS             complement(1473..2204)
                     /locus_tag="PKU88_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70007.1"
                     /translation="MRTYKRMYIKLMLSVTAVLGICFIALDFSRFLNQNYSYDIFPGA
                     ILKRICVIIAAVIAWSVGRDEINPKDSRLMKAAFIFVILGEAAFFIRERAIGIGMFAL
                     CQTLLTVRNGTGLRYKLARAGSNQKIGLIVSGIIVIISVIFPFLTGYLFKTDITLNIA
                     YLYGIILSISLWAGLACNILGLLPQRNAGMAALGMTCFFCCDVLVGLDAVLEAGLPWL
                     LANSFIWIFYIPALVLLALSCYRYN"
     gene            complement(2240..3010)
                     /locus_tag="PKU88_11875"
     CDS             complement(2240..3010)
                     /locus_tag="PKU88_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPX70008.1"
                     /translation="MQDTSELRFAVLIDAENIPYKNIRGILAEVAKYGTPTLKRIYGD
                     WTNSSLSGWKSNLLENAITPIQQYSYTTGKNSSDSALIIDAMDILYSEKVDGFCIVSS
                     DSDFTRLATRLREAGMKVIGIGERKTPSAFIAACDKFIYIEIINAPVKNSDQDSNELH
                     RDDTVEQDHKNSLDIGNDIIRLISDSVNDVADDNGWAFLADVGNLISKKQPDFDARNY
                     GFFKLTPLVKSLKCFQIEERESGNSRIKHIYIRNKNKQ"
     gene            complement(3013..3492)
                     /locus_tag="PKU88_11880"
     CDS             complement(3013..3492)
                     /locus_tag="PKU88_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPX70009.1"
                     /translation="MKTLIVYDSVFGNTEKIAEAIGDSLAELTGTEVEKVSEAKLEQL
                     QGTDLLIVGSPTRAFKPTKAIVDFLIQIPSNGLKGINVAAFDTRISTEDVNSRILNGF
                     VKIFGYAAKPIADKLQKKGGNLVAPPEGFFVKDNEGPLKEGELERAAKWAKQVMKGE"
     gene            complement(3576..3992)
                     /locus_tag="PKU88_11885"
     CDS             complement(3576..3992)
                     /locus_tag="PKU88_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPX70010.1"
                     /translation="MKSFRKELYFKLPVRRGYVNITNQIQACINESGIKEGFVLVNPM
                     HITASVFINDDESGLHQDFEKWLEALAPEKPHSQYRHNGFEDNADAHLKRQLMGREVV
                     AAVTEGKLDFGTWEQIFYGEYDGKREKRVLVKIIGE"
     gene            complement(4017..4421)
                     /gene="ndk"
                     /locus_tag="PKU88_11890"
     CDS             complement(4017..4421)
                     /gene="ndk"
                     /locus_tag="PKU88_11890"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020380556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPX70011.1"
                     /translation="MERTLVIIKPDVMERKLMGKIISIYEQEGLHISSIKILKPSVET
                     AESHYSEHKGKSFFIRLIEYITRGEVCVLIAEGDNAIDRARKINGATDPAEADKGSIR
                     GRYGVSKTENSVHASDSAESADREISIWFPEL"
     gene            complement(4734..4865)
                     /locus_tag="PKU88_11895"
     CDS             complement(4734..4865)
                     /locus_tag="PKU88_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70012.1"
                     /translation="MENAFNSFSEITGSARQELALENICKHLDDGGTFVCTLYNTDY"
     gene            complement(4999..5670)
                     /locus_tag="PKU88_11900"
     CDS             complement(4999..5670)
                     /locus_tag="PKU88_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPX70013.1"
                     /translation="MNPKKAVLFIGSPKERSASEALGAYLLGKLKEKGLSTDKIKIDK
                     LVSTERGQLELFDAVNQADIIIFSCPLYVDSPPAPVIKAMELITENRKGKGKENGKKF
                     LAISNCGFPEAFHNHTALEIYKEFASEAGFKWLGGLALGGGPAVQSSELTKIGGMAKN
                     IMRSLDTAANDIVEKEALSHEAVELIARPLVPAWMYLFIAGFGWKIQAKKHGVSRSIN
                     DCPYE"
     gene            complement(5667..5960)
                     /locus_tag="PKU88_11905"
     CDS             complement(5667..5960)
                     /locus_tag="PKU88_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70014.1"
                     /translation="MKMALDRLIPIVSPFFTKIQGEVHHKPRYKHYPSLVGIGILPEN
                     DQNKEKIFKTLVNKNAVNMHNPSWAACVFSGSQPAEQIRQQIKESLAKVGVIE"
     gene            complement(5970..6242)
                     /locus_tag="PKU88_11910"
     CDS             complement(5970..6242)
                     /locus_tag="PKU88_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPX70015.1"
                     /translation="MKAVILNGGKENDLSITEVLGTIVKELEATGADIRTFDLRDMNI
                     SRCLGCFNCWVKTPGVCIIDDDGRMIAKEAVQSDLVIFLTPIVFGG"
BASE COUNT         1669 a         1500 c         1103 g         2063 t
ORIGIN      
        1 gtacatctca ttatatataa tatacggtat tttttcaatc gttttctaat acatcactta
       61 ccgtggttat ttcatagccg tgacttgcca ggttctcaat caattggggc agtgcggcaa
      121 cggtaacctt agtcggatgc atgagtacaa tagctccttt atgatgcttg ttttccaccc
      181 gctgcaatat ctttttgtaa tccctggtat tccagtctat agtatctatg ctccacataa
      241 tgaccttgta acccaaggct tccgccgagc ttaccacagt accgtttata tcaccgtagg
      301 gaggggcaaa taggactgtt ttgtatcctg ttattctctt aataatctcc tccgtctttg
      361 atatttcctg cttgttcgat tcaggagtaa gcttgctgtg gtataaatgc ttgtagccgt
      421 gatttcccaa ctcatgtccg gctttatgca tgtttcgcag aagttcttca tttttctcag
      481 cccattgccc tccgataaag aaggtgattt ctatatcctt ttcttcgagc agctttagca
      541 tttgcggcag gtattcctct ccccatacca cattgcaggc aaaagcaatt tttttatcat
      601 cttccattcc tttatatatt gggtcataat aatgaagagt atttatgctg atggttctca
      661 atgcgaagat aaaaagcagc agggcggcta taattagaag catcgctaaa agttttaact
      721 tactgattat tattatcctg gatttcaaga ctatccctcc aaaataaaat attatagttg
      781 gtataataat taatattcag gcaggaagtc caaaatactt caatggcccc aaagcggcat
      841 aaaaaaaagc gtctctcgac gctgagacta ataaaaaaca tccgataacg gttgtttcta
      901 agtcgagcct cataagggcc attaacaatt tttgtataag tatgctctga ctatacatta
      961 ctgttcctat tttgagcttt gtctgcaaac tgagcggcct cactgccgca tggaattcta
     1021 ataatcatcc tttaagctta aaaacaatta tgcctgcaat cataattgct attcccagga
     1081 gtttggttat atcaaattca ataacagggc tgccgaacag gccgaaggaa tcaatgacag
     1141 ttgccgcaat aagctgtgta accaacagta ttgctatgct gtatgtcgca ccgagagccg
     1201 aaactgcaat catcgcgctg aatactataa ggactcccaa tacccctccc agaagataca
     1261 gtctgttcgt ctctccaatt ttggcaaagc ttccgtttcc ggcaatcatt aatattatta
     1321 atgaaaatac aaacccgctt ccgtgtacaa aggtgtttgt ttcccataaa cccgcttttt
     1381 ctcccaatct tgcattgaat acctgctgca tggcaataaa tattcctgct gcaattgcat
     1441 atatggtacc tttcattatg atactcctta aattaattat atctgtaaca gcttaaagca
     1501 agcaatacaa gggcaggaat gtaaaatatc catatgaagc tgtttgccag cagccatggc
     1561 aatcctgcct ccaatacggc atcaagcccc accaggacat cgcagcaaaa gaagcatgtc
     1621 attcccaaag cggccatccc ggcgtttctc tgaggaagca aacccagtat attgcaggca
     1681 agccccgccc ataatgatat actcaaaatt attccgtata aataggctat attcaatgta
     1741 atatccgtct tgaacaagta accggtcaga aacggaaata ttacggaaat aataactatt
     1801 atacctgaga caataagccc tattttttga ttactgcctg cccgggccag tttatatctt
     1861 aggcctgtac catttcttac agtcagcaag gtttgacaca gggcaaacat ccctatcccg
     1921 attgctcttt ccctgataaa aaaggctgct tctcccaaga taacaaatat aaaggcggcc
     1981 ttcatcaatc tgctgtcctt cgggtttatt tcatcccttc ccactgacca tgcaattacg
     2041 gcagctataa ttacgcatat tctcttaaga attgcaccgg gaaatatgtc atagctgtag
     2101 ttttgattca aaaaccttga gaagtcaagg gcaataaaac agatacccaa tactgctgtt
     2161 acggatagca tcagttttat atacattctc ttgtaagttc tcatattatc ccttctgctg
     2221 cttcccggaa atatgtgaat tactgcttgt tcttattccg gatatatata tgcttgattc
     2281 ttgaattacc cgactccctc tcttcgatct gaaagcattt gagactttta accagtggag
     2341 tcagcttgaa aaagccatag tttctggcat caaagtcagg ctgcttcttt gagataaggt
     2401 tgcccacatc tgccaggaaa gcccagccgt tgtcatcggc tacgtcattt acggaatcgg
     2461 atatcagcct tataatatca ttcccgatat ccagagaatt tttatgatcc tgttcaacgg
     2521 tatcatccct gtgcagctca ttgctgtctt gatcgctgtt cttgacaggt gcatttatta
     2581 tctcaatata tatgaattta tcgcaggctg caataaatgc gctgggggtt ttcctttccc
     2641 ctattccgat tactttcata ccggcttcac gaagccttgt agcgagcctt gtaaagtcgc
     2701 tgtcgcttga tactatgcag aagccgtcaa ctttctcgga atacagtata tccattgcat
     2761 caataatcaa tgccgagtcg gaggaatttt tccctgtggt atagctgtac tgctggattg
     2821 gtgttattgc attctccaat aagttactct tccagcctga cagtgaagaa ttggtccaat
     2881 caccgtatat acgcttcagg gtaggagtgc cgtatttggc tacctctgca agaatccccc
     2941 ttatattctt gtaggggata ttctccgcat ctataagtac cgcaaatctt aattctgatg
     3001 tatcttgcat cctcattctc ccttcataac ttgttttgcc catttggcgg cacgttcaag
     3061 ttctccttcc ttcaggggcc cttcattatc ctttacaaaa aaaccttccg gaggggctac
     3121 caggttcccg cccttcttct gaagcttatc ggctatgggt ttggccgcat aaccgaagat
     3181 ctttacaaat ccgttaagaa tccgggaatt cacatcttcc gtactgatcc tggtatcaaa
     3241 tgcagcaacg ttgattcctt tgagtccgtt ggaaggaatc tgaatcagaa aatcaactat
     3301 tgcctttgtc ggcttgaatg ctctggtggg tgaaccgaca atcagcaaat ctgttccctg
     3361 aagctgttca agctttgctt cgcttacctt ctcaacctca gtgcctgtca actccgcaag
     3421 tgaatcgccg attgcttcgg caatcttttc agtgttgcca aaaacagaat catatactat
     3481 caaagtcttc actgagttat ccccccttat ttaattttgg cctttcactc tatatattac
     3541 taatagtatc acttttatgc cgatttttga atgttttatt cacctattat cttgaccagc
     3601 actctttttt cacgtttgcc gtcatactcc ccgtagaata tctgttccca tgtgccgaaa
     3661 tcaagctttc cttcggtaac ggctgccacc acctcacggc ccatcaattg acgtttcaga
     3721 tgtgcgtcgg cattatcctc aaaaccgtta tgcctgtatt ggctatgggg cttttcaggg
     3781 gccagggcct ccagccattt ttcaaaatcc tggtgcagcc ccgattcatc gtcattgata
     3841 aatacgcttg cggtgatatg cataggattt accagtacaa acccctcctt tattccgctt
     3901 tcatttatgc aggcttgtat ttgattggtt atgtttacat agcccctgcg tacaggcagt
     3961 ttaaagtaca gttcttttcg aaacgatttc atctcaatac catcctttga acaaatttat
     4021 agctccggga accagatgct tatttcccga tctgcgcttt ccgcactgtc tgaagcatga
     4081 acggagtttt cagtttttga taccccatat ctgcctctta tgcttccctt gtctgcttcc
     4141 gccggatctg tcgccccgtt tatttttcgc gccctatcta tggcattatc cccttctgcg
     4201 attaatacac atacttctcc ccttgtaatg tactcaatta atcttatgaa gaagcttttg
     4261 cctttatgtt ccgaataatg agactctgcg gtttctacgg aaggcttcag tatttttatt
     4321 gaggatatgt gaaggccttc ctgctcataa atggagataa tttttcccat caattttctc
     4381 tccataacat ccggcttaat aattacaagc gttctttcca atatctcacc ccttctgttc
     4441 cttattcaat ataaagttca tgaacatgct gtcttccgag aacggagaaa aattatagtc
     4501 gccgaaaatt tcttttattt taaagccggc ttttgaagcc atctctatga aatcttcttt
     4561 gcttataagt gaaaaacaaa tatccaggca gcgtttttcc tttaatttat tatctttgtc
     4621 gtatatctca taaaattgta taccggttat atttttggtt tttccgttat agacattata
     4681 atatgaaatc actaagcttt tgttttcatc gatctgaaag cttcccagta cccttaataa
     4741 tccgtattat ataaggtgca aacaaatgtt cctccgtcat ccaggtgttt gcagatattc
     4801 tcaagagcca gttcctgcct tgcgctgccg gttatttccg aaaatgaatt aaaggcattt
     4861 tccattgcat tcctttgata gttttctata gaaatctttg tcaaagtcta cagtgacata
     4921 ggcatcatat aaatcagcca caaaattgaa atcaatgctt ttcataaaaa cctccttgcc
     4981 taagacatat tcaaaaaatt actcatacgg gcagtcatta atactccggc ttaccccgtg
     5041 ttttttggct tggattttcc atccgaatcc ggcaataaac aaatacatcc aagcgggaac
     5101 caggggcctt gctattagct caacagcttc atgactcaat gcctcttttt caacaatgtc
     5161 atttgctgcc gtatcaaggg atctcattat gtttttggcc attccgccaa tctttgttaa
     5221 ttcactgctt tgaacagccg ggccgccccc taatgcaagt cctcccagcc atttgaaacc
     5281 ggcctctgag gcaaactctt tatatatttc caaagcggta tgattatgaa atgcttccgg
     5341 aaacccgcaa tttgaaatag ccagaaactt ttttccgttc tctttgcctt tcccttttcg
     5401 attctccgtg attagttcca tagcttttat tactggagcc ggagggctat cgacataaag
     5461 cgggcaggaa aaaataatta tgtcagcctg attaactgca tcaaataatt caagttgtcc
     5521 tctctctgtg ctgaccagct tgtcgatttt aattttatcg gtactcaggc ctttctcctt
     5581 taacttacca agcagatacg ctccaagggc ttccgatgcg cttctttctt tggggcttcc
     5641 tataaataat acagcttttt tagggttcat tcaataacac ctactttcgc aagggactct
     5701 tttatttgct gccgtatttg ttcagcaggc tgactccctg agaaaacaca tgctgcccat
     5761 gatggattgt gcatattgac cgcatttttg tttacaaggg ttttaaatat tttttccttg
     5821 ttttgatcat tttccggaag tattcctatc cctacaaggg aagggtaatg tttataccgc
     5881 ggtttatggt gaacttcccc ctgtattttc gtgaaaaatg gagaaacaat cgggatcagc
     5941 ctgtctaatg ccattttcaa ttccggtgat tacccgccaa aaacaatcgg agtcaggaaa
     6001 ataaccaaat cgctttgaac tgcttctttt gcaatcattc gtccgtcatc atcaattata
     6061 caaactccgg gtgtcttaac ccagcagtta aagcagccca aacagcgcga tatattcatg
     6121 tccctgagat caaacgtcct aatatcagca ccggttgctt caagttcctt cacaatagtg
     6181 cctaatactt cagtaatact taagtcattc tctttacccc cgtttaatat taccgctttc
     6241 attccgcctt accccttttc tcaaaattac ctttttgctt tcattatttc agcccctctg
     6301 aagaatattc ttaaacagtt tggataaata tatct
//
LOCUS       DAOOAB010000081         6315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_14485_length_6315_cov_14.575399, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000081 DAOOAB010000000
VERSION     DAOOAB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14485_length_6315_cov_14.575399"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1116)
                     /gene="dusB"
                     /locus_tag="PKU88_11915"
     CDS             complement(136..1116)
                     /gene="dusB"
                     /locus_tag="PKU88_11915"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPX70016.1"
                     /translation="MQIGNVKLKNNVFLAPMAGVTDMAFRILCKRQGCGLTYTEMVSA
                     KGLHYKSDNTAVLLEIAEEEKPAAAQVFGSEPDIVAEAARQAEAGGAAIIDINMGCPT
                     PKIVRNGDGSALMKSPVLVGEIVRSTVAAVKVPVTIKIRKGWDEESVNAVEVASIAAM
                     EGAAAITVHGRTREQFYSGTADWNIIKRVKDAVDIPIIGNGDVITPQDAGRMLEETGC
                     DAVMIGRGAQGNPWIFERTAEYLKTGELLPEPDFEQRILTIMEHMDMVTELKGEGTGV
                     KEMRKHAAWYLKGMPGSAKVKAEIFKLTTCSQVKDILSQYLDYLQKHISS"
     gene            complement(1128..1898)
                     /locus_tag="PKU88_11920"
     CDS             complement(1128..1898)
                     /locus_tag="PKU88_11920"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPX70017.1"
                     /translation="MILVIDVGNTNIVLGIYDDRKLVSFWRVKTDAEKTSDEYGMIIN
                     QLFELQGHKFSDIEAVVICSVVPPIMYTLEHMTRKYFNREPLVVGPGVKTGMNVKYDN
                     PKEVGTDRIVNAVAAYELYGGPLIIVDFGTATTFCAVSKNGEYLGGAISPGIRISMDA
                     LFERTAKLPRVELAKPSTVICKNTINSIQSGVIYGYVGQVDYIVRRMKKELGDMNTKV
                     IATGGLSKLIASESETIDIVNGLITLEGLRIIYDRNRE"
     gene            complement(2030..3019)
                     /locus_tag="PKU88_11925"
     CDS             complement(2030..3019)
                     /locus_tag="PKU88_11925"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPX70018.1"
                     /translation="MKNRILEELRANSGQPVSGEEISRRLGVSRTAVWKHISKLRSEG
                     YSIESHTNSGYKLVGSPDILSSSELEPYLHTEFMGRNIIYLDSIDSTNTYAKKKAEGL
                     FREGTVVIADEQTGGRGRLGRHWVSTKGKGIWMTLMLKPDILPSDAPKLSIVAALAVT
                     NAIRSCCRLDAEIKWPNDIVAGGKKLCGILTEMSAEEDEIKYVIIGIGINANMDNDDF
                     GQEVSSIATSVRIESGMPVSRKALAASVLFEFEQIYRVFTRDGSIKAFLEDYKSKSAV
                     LGKEVRLISKKEEIKGLAVDISKEGHLVVRLADGSYREIMSGEVSIRGMHGYI"
     gene            complement(3149..4321)
                     /locus_tag="PKU88_11930"
     CDS             complement(3149..4321)
                     /locus_tag="PKU88_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPX70019.1"
                     /translation="MGFAAITWQQAVMLCLSCVLMYLALKKGFEPLLLVPIAFGMLLA
                     NLPVTGLMAGPVYEIATDPVTGEHVHQMKQAGGVLYYMYQGVKLGIYPPLIFFGVGAM
                     TDFGPLIANPRSILLGAAAQLGIFTTFIGAILLNLVPGLSFTLQEAGSIGIIGGADGP
                     TAIYLTAKLAPHLLGPIAVAAYSYMALVPLIQPPIMKALTTPEERAIVMEQLRPVSKI
                     EKIVFPILCTLVVSIILPSAAALIGLLMFGNLLREAVDLQSVLNRLSKTAQNELMNIV
                     VIFLGVAVGATANGEIFLRAQTLAIIALGLAAFSMGTAGGVIFGKIMCKMSGGKVNPL
                     IGSAGVSAVPMAARVSQKVGQEANPSNYLLMHAMGPNVAGVIGSAVAAGVLLAMLG"
     gene            complement(4426..4827)
                     /locus_tag="PKU88_11935"
     CDS             complement(4426..4827)
                     /locus_tag="PKU88_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPX70020.1"
                     /translation="MRKFMINVNGNSYEVEVEEIKEGAAQATAPVTAPRPAAPKPAAA
                     PAAAPKPAPAAAPAAAPAGANTVTAPMPGTILSINVKAGDAVKKGQVLCILEAMKMEN
                     EIMAGADGTVASVAVSQGDSVSTGQVLITFA"
     gene            complement(4852..5223)
                     /locus_tag="PKU88_11940"
     CDS             complement(4852..5223)
                     /locus_tag="PKU88_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPX70021.1"
                     /translation="MTNFSDTFTYALIVTLFGMGIVFLVLILLQYMLKAMEIIFHREK
                     KAVQEPAQVSAVKPAEPDEPQVNEAEEASDDQLAAVITAAVISCLGGNSNIAVRNIRR
                     VEDLTPAWGKASRTEQMAVRF"
     gene            complement(5245..>6315)
                     /locus_tag="PKU88_11945"
     CDS             complement(5245..>6315)
                     /locus_tag="PKU88_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPX70022.1"
                     /translation="QISVIMGPCAGGAVYSPALTDFTFMVDKTSQMFITGPQVIKAVT
                     GEEVSPEELGGAMTHNRTSGVAHFISSDDKACIQDIKRLLSFLPSNNLETAPIFETDD
                     DINRIEEQLNEIVPDNPNKPYDMRDIIRAIVDNGDFMEVQEYYAQNIITAYARINGAS
                     IGIIANQPKVLAGCLDINASDKAGRFIRTCDAFNIPILNLVDVPGFLPGTNQEYGGII
                     RHGAKMLYAYSEATVPKVTLIVRKAYGGAYLAMCSKDLGADMVFAWPSAEIAVMGPDG
                     AANIIFKKDIEGAEDPVQTRAEKIQEYKDKFATPYIAAKRGYVDSVIEPSESRPRLAN
                     AFEMLASKRESRPAKKHGNLPV"
BASE COUNT         1443 a         1716 c         1196 g         1960 t
ORIGIN      
        1 gacggggaca cggggacgtt tcgcgtgtcc cactggaagt gggacagggg aaacgtcccc
       61 atgtccccgc gtccccatgt ccccgtcgca ttattcttca gatgtgaaaa gggagtgccg
      121 caacactccc tttcactaag atgatatatg cttttgtaaa taatccaaat actgcgataa
      181 tatatccttc acttggctgc aggtggtcag cttaaatatt tccgccttta ctttggcgga
      241 acccggcatc cccttaaggt accaggctgc atgtttacgc atttccttta ctcctgttcc
      301 ttctccctta agctcagtga ccatatccat atgctccatg attgtcagaa ttctttgctc
      361 aaagtcaggc tccggcagca attcaccagt tttaagatac tctgctgtcc tctcgaatat
      421 ccacgggttc ccttgggcac cccttccaat cattaccgcg tcacaacctg tttcttcgag
      481 cattcttcct gcatcctggg gtgttattac atcaccgttt ccgattatcg gtatatcaac
      541 ggcatctttg actcgtttta ttatattcca gtcggcagtg ccgctgtaga actgctctct
      601 tgtcctgcca tggacagtta tcgccgcagc accttccata gcggcaatgg atgctacttc
      661 tactgcattg actgattcct catcccatcc cttcctgatc tttatggtga cgggcacttt
      721 gacagccgca actgtgcttc ttactatttc tcccacaagc accggacttt tcattagcgc
      781 acttccgtca ccgtttctga ctatcttagg tgtgggacac cccatattta tgtctattat
      841 tgccgctccg ccggcttctg cctgccttgc agcctctgcc actatatccg gctcgcttcc
      901 gaaaacctgt gctgctgcag gtttttcttc ctctgctatc tctaacagca cagcggtatt
      961 gtcactttta taatgcagcc ccttggcact gaccatttcc gtataggtaa ggccgcagcc
     1021 ctgccttttg cacagaattc tgaaggccat gtctgtaact cctgccatag gagctaaaaa
     1081 aacgttattc tttagcttta cattccctat ctgcataacg cccactatca ttccctgttt
     1141 ctatcatata ttattctcaa accctcaaga gtaatcaagc cattcactat atcaatggtt
     1201 tcggattctg aagctattaa cttcgaaagt cctccggttg ctattacctt tgtattcatg
     1261 tccccaagct cctttttcat acgccttaca atataatcca cctgtcccac atatccgtat
     1321 attacccccg actgtatgct gtttattgtg ttcttgcata ttacagtact gggctttgca
     1381 agctcaaccc tgggcagttt ggcagtcctt tcaaaaagtg catccataga tattctgata
     1441 ccgggggata ttgcacctcc aagatattct ccgttttttg acactgcgca aaatgtggtc
     1501 gccgtaccaa agtcaacaat tataaggggc ccgccataaa gctcgtatgc ggcaacggca
     1561 ttgactatcc tgtcggttcc cacttccttg ggattatcat attttacgtt catgcctgtc
     1621 tttacacccg gtcccacaac cagcggctcc ctgttgaaat atttccttgt catgtgttca
     1681 agggtataca tgatgggagg tacaacagag catatcacca ctgcctcaat atcagaaaat
     1741 ttgtgtccct gtaattcgaa cagctggttt attatcattc cgtattcgtc agaggtcttt
     1801 tccgcatcgg tttttactct ccaaaaactg acaagcttcc tgtcatcata tattcctaat
     1861 acaatattgg tatttccaac gtctataaca agtatcatat aaatcctccc atatctattg
     1921 gttattatcc tgcataaagc tgcagggcac cagttccgca ttaattttag caccgcttca
     1981 ctactttttc aacaaatatg ttctgcccaa taaacaacct gcatcaaact catatatatc
     2041 catgcatgcc ccttatcgaa acctctccgg acattatctc cctgtatgaa ccgtctgcaa
     2101 gccttacaac aaggtggcct tccttggata tatccaccgc cagcccttta atttcctcct
     2161 ttttgcttat cagccttact tcttttccca ataccgcaga cttactttta taatcttcca
     2221 gaaaggcctt gatgcttcca tctcttgtaa aaaccctgta aatctgctca aactcgaaca
     2281 gcacggaagc tgccagggct tttctggata caggcatgcc gctttcaatt cttaccgatg
     2341 tggcaatgct tgatacttcc tgaccgaagt catcattatc catatttgca tttattccaa
     2401 ttcctataat aacatatttt atctcatctt cctccgcact catttcggta agtatcccgc
     2461 aaagcttttt gcctcctgcg actatgtcgt tgggccattt tatttctgca tccagccggc
     2521 agcagcttcg gattgcatta gtaacagcaa gggcggcgac aattgacagc tttggtgcat
     2581 cggaaggcaa tatgtcaggc ttcagcataa gggtcatcca aattccctta cccttagtgg
     2641 aaacccaatg ccttccaagc cttccccttc cgccggtctg ttcatcagct ataactacgg
     2701 taccttcccg gaataaacct tcagcttttt tctttgcata tgtattggta gagtcaatac
     2761 tgtcgagata aattatattc ctgcccatga actcagtatg taagtaaggt tcaagttcgc
     2821 ttgatgacag gatatcaggg ctccctacca gcttataccc gctgttggta tgggattcta
     2881 tgctgtagcc ttcactgcga agcttgctta tatgcttcca aactgcagta cgggataccc
     2941 ccagccttct gcttatctct tcgccggata ccggctgacc gctatttgcc cttaattctt
     3001 caagtatcct gtttttcata ctttcacctc attgcccgtc catgctcaaa gcttggctcg
     3061 tgctcttcga gtcttatata ttatattcta ctataacttc aataaaaaat cagacaattt
     3121 cttgtctgat tttatacatt gtctgctatt atcccagcat ggccagaagc actccggctg
     3181 caactgcaga gcctataact ccggctacgt ttggtcccat tgcatgcatg agaaggtaat
     3241 tgcttggatt tgcctcctgc cccaccttct gtgataccct tgccgccatt ggcactgccg
     3301 atactcctgc ggagcctatc agtggattta ccttcccgcc tgacattttg cacattatct
     3361 ttccgaatat tactccgccg gctgttccca tggagaatgc cgcaagcccc aatgcgatta
     3421 ttgccagcgt ctgcgccctt aagaatatct caccgtttgc agttgcacct accgcaactc
     3481 caaggaatat tactactata ttcataagct cattctgggc tgttttggaa agcctgttca
     3541 atacactctg cagatcgact gcttccctaa gcaggttgcc gaacataaga agcccgataa
     3601 gcgctgctgc cgatggaagt attatcgata ctaccaaggt acagagtatt ggaaatacta
     3661 tcttttcaat ctttgatacc ggcctcagct gttccattac tatagctctt tcctcaggag
     3721 ttgtcagtgc tttcattata ggaggctgga ttagcggtac cagagccatg tacgaatatg
     3781 cagctactgc tatcggtccc aaaagatgcg gggccagttt tgccgtaagg taaatggctg
     3841 tcggtccgtc agctccccct attatgccta tggagcctgc ttcctggagg gtaaaggata
     3901 atcccggaac aagattcaat agtattgctc cgataaaggt tgtaaatata cccaactgtg
     3961 ctgctgctcc cagaagtatg ctcctgggat ttgctataag cgggccgaag tctgtcattg
     4021 cacctactcc gaagaatatc agcggcgggt atattcccag cttgacaccc tggtacatat
     4081 agtacagtac tcctcccgcc tgtttcattt gatgcacatg ctcccctgtt accggatctg
     4141 ttgcgatttc atatacagga cctgccataa gccctgtcac gggaaggttc gccagcagca
     4201 tgccaaatgc tatgggcacc agcagaagcg gttcaaatcc cttcttcaat gcaagataca
     4261 taagcacgca ggataaacaa agcatgactg cctgctgcca ggttattgcc gcgaatccca
     4321 tactataata aaagtttaat aattcatcta ataatgacac ccggattccc ccaatcattc
     4381 tttaaacttt gcaggaattt tcttaattcc cctgtttcaa taacactagg caaatgtaat
     4441 caatacctgt cctgtgctta cagaatcccc ctggcttaca gctactgatg ctactgtgcc
     4501 gtctgcccct gccattatct cgttttccat cttcattgcc tcaagtatgc aaagaacttg
     4561 tccctttttt accgcatctc ctgcttttac attaatgctg agtatcgtac ccggcatcgg
     4621 tgctgttact gtatttgccc ctgcaggtgc tgctgccggt gctgccgcag gcgcaggttt
     4681 cggcgctgct gcaggtgctg ccgcaggctt tggcgctgcc ggccttggtg ctgttaccgg
     4741 tgctgttgcc tgtgctgcac cttcctttat ttcttctact tcaacctcat agctatttcc
     4801 attaacattt atcataaatt ttctcatgaa attttcctcc ttaattaata tttagaacct
     4861 gacagccatc tgttccgtcc tgcttgcctt accccaggcc ggagtcagat cctcaacccg
     4921 tctgatattt cttactgcaa tattgctgtt ccctcccaga caggatatta ccgcagcggt
     4981 tattacagct gccagctgat cgtctgaagc ttcctccgcc tcattcactt gcggttcatc
     5041 tggctcagcc ggttttactg cagaaacctg agcaggttcc tggactgctt tcttctccct
     5101 gtgaaatatt atttccatgg ctttcagcat atactgcaga aggatcaata ctaaaaatac
     5161 tatgcccatt ccaaaaaggg tcactatcaa ggcatatgta aaggtatcgc tgaaattagt
     5221 cattatctca ccccatcctt cttattatac aggcaggttg ccatgctttt tggcgggcct
     5281 gctttctctc ttgcttgcaa gcatttcaaa tgcattagct aacctcggcc ttgactcgga
     5341 gggttctatg accgaatcaa cataacctct ttttgctgct atatatggtg tggcaaactt
     5401 gtctttgtac tcctgtatct tttctgcccg ggtctgtacg gggtcttctg ccccttctat
     5461 atccttcttg aatattatat tggctgctcc gtcaggcccc ataaccgcga tttcagctga
     5521 aggccatgca aataccatat ctgctccaag atccttgctg cacatggcaa gatatgcccc
     5581 tccgtaagct tttcttacta tcagtgtaac ctttggaact gtggcttccg aatatgcata
     5641 aagcatcttg gctccgtgcc ttatgatccc tccgtattcc tggtttgttc ccggaaggaa
     5701 gcccggtacg tctaccagat tcagtatcgg aatattgaag gcatcgcagg ttcttatgaa
     5761 tcttccggcc ttatccgaag cgtttatatc cagacagccc gccagaacct tgggctgatt
     5821 tgctattatt cctatgcttg ctccgtttat tcgggcataa gcagttatta tattctgtgc
     5881 atagtactcc tgcacttcca tgaaatctcc gttatccaca attgctctta tgatatccct
     5941 catatcgtaa ggcttgttgg gattatccgg tactatttca ttcaattgct cttcgattct
     6001 atttatatca tcatcggttt caaatatcgg cgcagtctcc agattgtttg aaggcagaaa
     6061 gctcaaaagc ctctttatgt cttggatgca ggccttatca tcagagctta tgaaatgtgc
     6121 cactccgctt gttctgttgt gggtcatggc tcctcccagt tcctccgggg agacctcttc
     6181 ccctgtaact gcctttatga cctgtggccc tgttatgaac atctggcttg tcttgtccac
     6241 catgaatgta aaatctgtaa gggcaggaga gtaaaccgct ccccctgcac aggggcccat
     6301 aattaccgat atctg
//
LOCUS       DAOOAB010000082         5966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_15459_length_5966_cov_16.627474, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000082 DAOOAB010000000
VERSION     DAOOAB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15459_length_5966_cov_16.627474"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKU88_11950"
     CDS             <1..931
                     /locus_tag="PKU88_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPX70023.1"
                     /translation="GIYLGCCGAPADWAGRQDLMQENIEIIKKTWEDTGRPVFILACS
                     SCCSVFEKYLPEIAFVSLWEIFARKGLPQNNRIPGKRVLSVHDACATRHNKEIHDSIR
                     RIAVLSGYEIQELKYSKEKTKCCGYGGLVYFANREQSADFVKHRIEESDNDLLVYCAM
                     CRDLLAEGGKRTFHILDLIFAEDMERAALRKMPTLSERHGNRAQVKIGLLREIWGEEP
                     DMDYEKQRDFKLIIPENVRQQMEDRYILSEDMAEVIDSSRKSGERFFNPQENSCLARL
                     RLDNVTYWVKYEEKDEKILVKSVYSHRMEIVEE"
     gene            934..1140
                     /locus_tag="PKU88_11955"
     CDS             934..1140
                     /locus_tag="PKU88_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPX70024.1"
                     /translation="MNEDKNYNMPWLCDKCKRELVLKKVKVRYLEGNFEVDLPKCPEC
                     NMVFIDEEMAVGKMLEVEKELEGK"
     gene            1161..1610
                     /locus_tag="PKU88_11960"
     CDS             1161..1610
                     /locus_tag="PKU88_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPX70025.1"
                     /translation="MNDIAFKLLKLSSSGFCCTQIMLKLTLDEEGKENEDLLKAVSGL
                     CRGIGGRQKSCGVLTGGLGILGLYAGKGKDTEYPKQNYSTMTEQYLEWFESEFGSEEC
                     RDIIGVYDFSDDTGNSYMIKCGDILLKSYEKVVEILRDNDYEYGSRE"
     gene            1613..2923
                     /locus_tag="PKU88_11965"
     CDS             1613..2923
                     /locus_tag="PKU88_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPX70026.1"
                     /translation="MKQTPIEQWIMQKTGIKERDKKALEAYQLGKIKESIAYVKENSI
                     FYCKQLKNIREESINSPESFQNIPFTYPRDIKRNPPDFLCVPQRKVARIVTLKTSGTS
                     GEDKRLFFTEEDIELTIDFFRHGMSCLVDKSDKVLVLMPGSTYGSIGDLLKKALALSD
                     IECIVHGVLTDPDEAARLIEEKGITCIVGIPLQVLYLSRVKSNVFKNSIKKVLLSTDY
                     VPDVLTNELERRFGCRVFDHYGMTEMGYGGGVECEALNGYHMREGDMYFEIIDPDTGK
                     AVECGQYGEVVFTTLTRQAMPLIRYRTGDIASFSSNPCACGTFLNTMKKVLGRIENRV
                     RIGEERFLYLRELDEIILPLEEVLDYRAYLDDKDRLVLEVTVKDGIDFTEIKKEVMRS
                     IRNLLSNQSKCGLSVEIVKAAVNEPASITDSMVKRKIIDYRKER"
     gene            2927..3985
                     /locus_tag="PKU88_11970"
     CDS             2927..3985
                     /locus_tag="PKU88_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPX70027.1"
                     /translation="MKDIYELINEQLDKKESFVLATILKKLGSAPREEGTKMLIRKDF
                     SIEGTIGGGISEAMTIKLSAEVFEDGLYIIENFLMSNKDSACLGMVCGGDISVLLEYV
                     DCNDSQMVDIYRKAIELKHCGTDFVMITKLSEDRRKIRGSDKWICTETGFYGMENEEI
                     WETIKNIRVNFKETKIREIFIGKERYIIEPFFNVESVYIFGAGHVAQKIADITKMLGF
                     YTVVMDDREEFANRERFETADEVRVIQSFNNLENYDIINGNSYIVIVTRGHVYDKDVL
                     AQMLRTDARYIGMIGSRSKREHVYNNLLSEGFTSEDLERVHCPIGLNIHAQTPEEIAV
                     SIAAELIKVRREPKDKTH"
     gene            4025..5911
                     /locus_tag="PKU88_11975"
     CDS             4025..5911
                     /locus_tag="PKU88_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX70028.1"
                     /translation="MNNIDYKHVIELSHERCRKFNIKEDQVYSSRIINETELQKRFAE
                     NRSMILTAAPYMEQLINFVKGHNFFALLTDGEGCILNAIGDEKILSEAFDLKMIPGAF
                     MNEESIGTNAMGIVIKTGEPVQLSGKDHFVKAYHRWTCSGAPIKDHNGNLIGVLDLTG
                     YTEYVHPHTLGMVIAASNAIEEMIKVKEFNNLQDINNKHIKTIFNTMPVAIVTSDMYG
                     KIKIYNNKALELLDNKDNQLKAKYMSEIIEEWDRIKQDIYQGRNISQEVNIIVFRNRL
                     SCRLIANPIYNSVDDSVEIVYALEEIKKEKRDKKKSEYQAYYTFDKIIGKDENFLKIV
                     EYAKKISDSKSTILIMGESGTGKEVFAQAIHNFSKRMDGPYLALNCGAIPNQLIESEL
                     FGYEEGAYTGAKKGGNVGKFELASGGTIMLDEIGEMPLDMQTKLLRVLQEGIITRIGS
                     AKPIPVDVRVIASTNKDLKKEVQLGRFRKDLFYRLNVLPIFLPALSQRKGDILLLTDY
                     FMKKIAEKLGKKEVKIPKGYIKSMMEYDWPGNVRELENFIELIIYSESIPYDYFNQEK
                     SFAEELYDNYIQGSDMNLCNMEKAHLKKVLKKHNGNITRSAESLGISRNTLYSKMKKY
                     RVKV"
BASE COUNT         2041 a          924 c         1499 g         1502 t
ORIGIN      
        1 cggaatttac ctgggatgct gcggggctcc tgccgattgg gcagggaggc aggatttaat
       61 gcaggagaac attgagataa ttaaaaaaac atgggaagat acgggcaggc ctgtttttat
      121 ccttgcctgc tccagttgct gcagtgtatt tgaaaaatat cttccggaaa ttgcttttgt
      181 ttccttatgg gagatatttg cccggaaagg tttgcctcaa aacaacagaa ttccgggtaa
      241 acgcgtttta agcgtgcacg atgcatgtgc tacaaggcat aataaggaaa tacacgacag
      301 tataaggaga atagctgttt tatcaggcta tgaaatacag gagttaaaat attcaaagga
      361 gaaaaccaaa tgctgcggat atgggggact ggtgtacttt gccaacaggg aacagtctgc
      421 agactttgta aagcatagga ttgaagaaag cgataatgac ctgctggtat attgtgctat
      481 gtgcagggat ttgcttgcgg agggcggaaa aagaaccttt cacattcttg atttaatatt
      541 cgcagaggat atggaaaggg ctgcactcag aaaaatgccc actttgtctg aacggcacgg
      601 gaacagggcg caggtaaaaa tcggattatt aagagagata tggggtgaag agcctgatat
      661 ggattatgaa aaacaaaggg atttcaaatt gataatacct gaaaatgtgc ggcagcaaat
      721 ggaagacaga tacattctat cggaggatat ggcagaggtt attgacagtt caagaaaaag
      781 cggagagagg ttctttaatc ctcaggagaa cagctgcctg gcaaggttga gacttgacaa
      841 cgtcacctac tgggtaaaat atgaggaaaa ggatgagaag attctggtaa agagtgtata
      901 cagccacaga atggaaatag tggaggaata ggaatgaatg aggataagaa ttataatatg
      961 ccgtggctct gcgataaatg caagagggaa ctggtgctaa aaaaggtaaa ggtacggtat
     1021 ttggagggga atttcgaagt tgatcttcca aaatgccctg agtgcaatat ggtttttata
     1081 gacgaggaaa tggctgtggg caagatgctt gaggtggaaa aagagttgga aggcaaataa
     1141 ggtgaaggga gatgccgatt atgaatgata ttgcattcaa actgctcaag ctgtcatctt
     1201 caggtttttg ctgtacgcag ataatgctta agctgacgct ggatgaagaa ggaaaggaaa
     1261 atgaggattt gctgaaagct gtaagcggat tgtgcagggg tatagggggc aggcagaaga
     1321 gttgcggagt gctgaccgga gggcttggaa tccttggcct ttatgctggg aagggaaagg
     1381 atacggaata tcctaaacaa aactattcca ctatgaccga acagtacctg gaatggtttg
     1441 aaagtgagtt cggaagtgaa gaatgcaggg atattattgg cgtttatgat ttttccgatg
     1501 atacaggaaa cagttatatg ataaagtgcg gggatatttt gttaaagagc tatgagaagg
     1561 tagtggaaat attgagagac aatgattacg agtacggaag cagggaatga ctatgaagca
     1621 gacacctatt gaacagtgga ttatgcaaaa aacaggaata aaggaaaggg acaaaaaggc
     1681 gttggaggct tatcagctgg ggaaaataaa ggaatcgatt gcctatgtaa aggaaaacag
     1741 cattttctac tgtaagcagt tgaaaaatat ccgggaagaa tcgattaatt ctccggagag
     1801 ttttcaaaat atccccttta cctatccccg ggatattaag agaaaccccc cagatttttt
     1861 atgcgtgcct caaagaaagg ttgcaagaat tgtgacatta aagacctccg gtacaagcgg
     1921 tgaggataaa agactgtttt ttaccgaaga ggatattgaa cttaccattg attttttcag
     1981 gcatggcatg agctgccttg tcgataaaag cgataaggtg ctggtactta tgcccggcag
     2041 cacttacgga agcatcggag atttattgaa aaaggcattg gctttatcgg atatagaatg
     2101 tattgtacat ggagttctta ccgacccgga tgaagcagca aggctaattg aagaaaaggg
     2161 cataacctgc atagtcggaa tacctttgca ggtgctgtac ttaagcagag ttaaaagtaa
     2221 tgttttcaag aatagtataa agaaggtgct gctgagcaca gattatgttc ccgatgtact
     2281 aaccaatgag ctggaacgca gattcggctg cagagtattc gatcactacg gaatgacgga
     2341 aatggggtac ggcggaggtg ttgaatgcga ggctctaaac ggataccaca tgagagaagg
     2401 ggatatgtat tttgaaatta ttgatccgga taccggtaag gctgttgaat gcggacaata
     2461 tggtgaggtt gtttttacaa cccttacaag gcaagctatg ccgctgattc gatacagaac
     2521 aggggatatt gcttcattct cttcaaatcc atgtgcttgc gggacatttc tcaacacaat
     2581 gaaaaaggtg cttggaagaa tcgaaaacag ggtcagaata ggagaagaaa ggttcctgta
     2641 tttgagggaa ctggatgaaa tcattctgcc ccttgaagaa gtcctggact atagggctta
     2701 cttggatgac aaagacagat tagtgctgga agtgactgtg aaagacggta ttgattttac
     2761 agaaataaag aaagaagtaa tgcggagtat ccggaatctg ctgtcaaatc aatcgaaatg
     2821 cggattaagt gtggagattg tcaaagcagc agttaatgaa cctgcaagca taaccgacag
     2881 catggtaaaa aggaaaataa tcgattatcg caaggagaga tgaagattga aagacatata
     2941 tgaacttatc aatgaacagc tggataaaaa agaaagcttt gtgctggcta ccattcttaa
     3001 aaaactcggt tccgcaccca gggaggaagg cacgaaaatg ttgataagga aggacttttc
     3061 aattgaagga accataggcg gcggaatttc cgaggctatg accataaagc tctcggctga
     3121 ggtatttgaa gatgggctat atattattga aaattttttg atgtccaaca aagactccgc
     3181 atgcctgggc atggtatgcg ggggagatat aagcgtactg ctggagtatg tggattgcaa
     3241 cgacagtcag atggtcgata tatacaggaa agcaatcgag ttgaagcact gcggcacgga
     3301 ttttgttatg ataacaaaac tatcggaaga taggagaaaa atcaggggtt cggataaatg
     3361 gatctgcacc gaaacgggat tctacggtat ggagaatgaa gaaatatggg aaacaataaa
     3421 gaatatcaga gtaaacttta aagaaaccaa gatcagggag atattcatcg gaaaagaaag
     3481 gtacataatc gagccgtttt ttaatgttga aagtgtttat atctttggtg caggccatgt
     3541 agcgcaaaag attgcagata taacaaaaat gcttggcttc tatactgttg ttatggatga
     3601 cagggaggaa tttgcaaaca gggaaagatt tgaaacagct gatgaggtta gggtgataca
     3661 atcttttaat aatttggaaa attatgatat aattaatgga aacagttata tagtaattgt
     3721 aacaagggga catgtctatg ataaggatgt gcttgcccaa atgctcagaa ccgatgccag
     3781 gtatatcgga atgatcggca gcagaagcaa aagggaacat gtctataaca atcttttgag
     3841 tgagggcttt acttcagagg atttggaaag ggttcactgt ccgataggat tgaatattca
     3901 tgcccagacc ccggaggaga ttgctgtgag cattgcagcg gaattgatta aggttagaag
     3961 agagcctaaa gataaaacgc attaaagccg gaaacgagaa actattacat aaaggagaat
     4021 acatatgaat aatattgatt ataagcatgt aatagagctt tctcatgaac gatgcagaaa
     4081 atttaatatt aaagaggatc aggtttacag cagcagaatc attaatgaaa cggagctgca
     4141 aaaaagattt gcagaaaata gaagcatgat tttaacggca gcgccttata tggagcagtt
     4201 aataaatttt gtgaagggcc ataatttctt cgctcttctt actgatggag aagggtgtat
     4261 actgaatgcc atcggagacg aaaaaatact gtcggaagct tttgatttaa aaatgatacc
     4321 cggggctttt atgaatgagg aaagcatcgg aaccaatgca atgggtattg ttataaagac
     4381 cggcgaaccc gtgcagttgt cgggtaaaga ccattttgta aaggcatatc acagatggac
     4441 ctgttccgga gcacctatca aggatcacaa cgggaacctt attggcgttt tggatcttac
     4501 aggctatacc gagtatgtac accctcatac cttgggaatg gtcatcgcag cttccaatgc
     4561 aattgaagaa atgatcaagg tcaaggagtt caataatctg caggatataa acaacaagca
     4621 tataaaaacg atattcaata ctatgcctgt tgctattgta acctccgata tgtacggcaa
     4681 gataaaaatt tataacaata aagccctgga gcttttggat aacaaggata accaactgaa
     4741 ggcaaaatat atgagtgaaa ttattgagga gtgggatagg atcaagcaag atatctatca
     4801 agggagaaat atatcacagg aagtaaatat cattgtgttt agaaacaggc tctcttgccg
     4861 tttgattgcc aacccaatct ataattcagt tgatgacagt gtagagatcg tatatgcttt
     4921 ggaagaaatt aaaaaggaaa aaagagataa aaagaaaagc gaataccagg cttactacac
     4981 ctttgacaaa ataataggaa aagacgagaa ttttttgaaa attgttgagt atgcaaaaaa
     5041 gatttcagac agcaagtcca caatactaat aatgggcgaa agcggtacag ggaaagaagt
     5101 atttgctcaa gcaattcata attttagtaa aagaatggac ggtccttatc tggctttgaa
     5161 ctgcggcgcc atacccaatc agcttattga atcagagctt ttcggatatg aggaaggcgc
     5221 ttataccggg gcaaaaaaag gaggaaatgt cggcaagttc gaattggcct cagggggcac
     5281 gattatgctg gacgagatag gtgaaatgcc tctagatatg caaaccaagc ttctgagagt
     5341 ccttcaggaa ggcattatta caagaatcgg cagcgcaaag cccatacctg tggatgtcag
     5401 ggtaattgcc tctacaaata aggacttgaa gaaggaagta cagcttggga ggttcagaaa
     5461 ggacttgttt tacaggttga acgtactccc catattttta ccggccttga gccagagaaa
     5521 aggtgatatt ttgctgctta cagattattt catgaaaaaa atcgctgaaa agctcggcaa
     5581 aaaagaagta aagattccaa aaggatatat taagtctatg atggaatatg attggcccgg
     5641 gaatgtaaga gaactggaga attttataga gttgattatc tattccgaat ctattcccta
     5701 tgactatttc aatcaggaaa aaagctttgc tgaagagctt tatgacaatt acatccaagg
     5761 ttcggatatg aacctttgca atatggaaaa agcacatctg aagaaggtac tgaaaaaaca
     5821 caacggaaat attacccgtt ccgcagaatc cttaggcata agcagaaata cactgtacag
     5881 taaaatgaaa aaatataggg taaaggtata actctttact ttcatttccc atttcacatt
     5941 acaacccata tttgtatatc cttaaa
//
LOCUS       DAOOAB010000083         5941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_15540_length_5941_cov_14.927115, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000083 DAOOAB010000000
VERSION     DAOOAB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15540_length_5941_cov_14.927115"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1303)
                     /locus_tag="PKU88_11980"
     CDS             complement(74..1303)
                     /locus_tag="PKU88_11980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPX70029.1"
                     /translation="MNKTTKALISLGVNCLLVIIAYFVSVWLKAAPPTIKLLSQYVSY
                     VLFAAAMFFGWWFNRSRVFFCSLTLMLCQLALFQLIPQEPDAAEYFYMILSMTSVYIP
                     LNILVFSIQRERGIASSWGRMRLLFIAAELLIGAWIIWYNDSDLLQLFNIKLFPADIS
                     FIPDLSHMSLLLYFITFIFLFGRQAVTGKNHDTPFMGITLFSFAALMFNKDLNTASVM
                     FALSGLMLSAVVIRASHSMAYLDELTGLPSKRALKEEMLKLGSKYTIAMLDIDYFKKF
                     NDTYGHDVGDDVLKLVAACIKGVGGGGKAFRYGGEEFTIVFPGKTAEEAIPYLEELRE
                     YIANRGFIRRSKSRPKEKPADIVTRSSSYKKLYLTVSIGAAEKNEKYRTSEEVIKAAD
                     KALYNAKSKGRNCVCKS"
     gene            complement(1305..2054)
                     /locus_tag="PKU88_11985"
     CDS             complement(1305..2054)
                     /locus_tag="PKU88_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HPX70030.1"
                     /translation="MSSGKKEFFEKDLYKPIYDYLAGLGYTVRSEVNHCDIAAIKENE
                     LLVVEMKKALNLDVILQAVQRQKLADMVYIAVPKPGRGLFSKRWQNLCYMLKRLQLGL
                     MVVSLKEGCSFVEVVFEPAAFDMAKSKNHSKRKRQELVKEFAARYGDFNTGGSTREKL
                     VTAYREMAIHIACCLMRYGQLKPRELRQLGTDEKKTTAILQKNHYGWFENISKGLYAI
                     NDKGRQEVSEYTELYQYYMHLAEEKSESEDK"
     gene            complement(2298..3569)
                     /locus_tag="PKU88_11990"
     CDS             complement(2298..3569)
                     /locus_tag="PKU88_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPX70031.1"
                     /translation="MEVLTLGSRGAAVKLIQSLLIRIGYNPGPVDGIFGPVTREAVRE
                     FKLDNGLEPDGVAGPAAWSRFERFLRGYDTYTIRPGDTFYNISGRYYTTLNAVMTANP
                     GVDPGNLRVGQVINVPYGIDVVFTDIDYTYEIMDRDIRGLKVRYPFIEVGIAGRSVLG
                     KNLYYIKLGNGPSEVFYNGAHHALEWITAPLLMKFIENYARGYARNSGIQGYNIRDLW
                     NRGSIYIMPMVNPDGVNLVLEGLKRDNPYYDRLLAWNDTGLPFSQVWNANIRGVDLNR
                     NYPASWEEAKAQEPSLGVDGPGPTRYGGPSPLSEPETQAVVNFTRQHNFKLVIAYHTQ
                     GRVIYWLYKGIRPPRALEIANIFSEITGYAVSGVPYEAAYAGYKDWFVEQYRRPGYTF
                     EVGIGRNPIPISQFDTIYRENEEVLLLAPIV"
     gene            complement(3643..4737)
                     /locus_tag="PKU88_11995"
     CDS             complement(3643..4737)
                     /locus_tag="PKU88_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPX70032.1"
                     /translation="MKKTSVIVITLVILLLVLSGCGKAPELVLTKYSDTFFGTFDTLT
                     QVVGYNKSEQEFSEYFEKIRARFEELHKLYDIYNDYEGINNIKTINENAGIKPVKVDK
                     EITDLILFAKEWHAKTGGRTNIAMGAVLSIWHDYRERGEGDPVNAELPPLEKLREASN
                     HTDINKVIVDEANSTVFLSDKEMRLDVGAIAKGFATELVAKEIMSEGFTSGIISPGGN
                     IRVLGKPLDGMREYWGIGIQDPDKMFIAAEENLLDTIFVNNAAIVSSGDYQRYYVVEG
                     KIYHHLIDPDTLMPGEYYRAVTIVTEDAGLADFLSTTAFLLPYDKSRKLVESLDNVEA
                     VWVMPDGKVEATDGMKKLMKSHGATGSKAE"
     gene            complement(4921..>5941)
                     /locus_tag="PKU88_12000"
     CDS             complement(4921..>5941)
                     /locus_tag="PKU88_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70033.1"
                     /translation="PAEPAAPAAEGGSIVKLGIGHITTIAKSKDLGKDAEGKEVLPLG
                     QVDTVMAAVGFDKDGKVISVTIDNAQTKVTFDAALQVTSDIKAESKTKVELKEEYGMI
                     KASSIGKEWYQQAEALGQWMVGKTVDEIKAMKTKNKDASHPAVPDVPELTSSVTVSVQ
                     EYIAAVAEAYETAVDVAGGEKVGLGHVITTGKSKGLGKDAEGKEVLPFAQVDTVMAAT
                     AFDKDGKVVGTIIDNAQTKITFDNTGKVTADKAAEYPTKVELKEGYGMIKASSIGKEW
                     YQQADALGQWMVGKTVDEIKAMKTKNKDASHPAVPDVPELTSSVTVSVQEYIAAVAEG
                     YANAK"
BASE COUNT         1563 a         1537 c         1073 g         1768 t
ORIGIN      
        1 ggacgtttct cctgtcccgc tgaaagcggg acaggagaaa cgtccccgtg tcccaaacgt
       61 ccccatgtcc tggtcaagat ttacacacac agtttcttcc tttcgattta gcattgtaca
      121 atgctttgtc tgctgcctta ataacctctt ccgacgttct gtatttttca tttttctccg
      181 ccgctccgat acttaccgtc aaataaagct ttttatatga actgcttctt gtcacgatat
      241 cagcaggctt ttcttttggc ctgcttttac ttctccttat gaaacctctg tttgcaatat
      301 attcacgcag ctcttccaga tatggtattg cttcctccgc agtcttgccg ggaaaaacta
      361 ttgtaaactc ctcccctcca tatctgaagg ctttccctcc gcctccgaca cctttaatgc
      421 aagccgccac aagcttgagc acatcgtctc ccacatcatg gccgtatgta tcattgaatt
      481 tcttaaaata atcaatatca agcatagcta tggtatattt gcttccaagc ttaagcattt
      541 cctccttcaa agcccttttt gagggcagtc ctgtaagttc atccaaataa gccatgctgt
      601 gggatgcccg gatcaccacc gctgacagca ttagcccgga tagtgcaaac atcactgatg
      661 cggtatttaa gtccttatta aacatcagtg cggcaaagga aaataatgtt atacccataa
      721 aaggtgtgtc atgatttttc cccgtaacag cctgtctgcc aaaaagaaat ataaaagtaa
      781 taaaatacag cagcagtgac atatgagaaa gatccggaat aaaacttatg tcggccggaa
      841 acagcttaat attgaaaagc tgaagtaagt ccgaatcgtt gtaccatatt atccaagcgc
      901 cgattagaag ctccgcagca ataaacaaca gcctcatcct tccccaggat gacgctatgc
      961 ccctctctct ttgaattgag aaaaccagaa tattaagggg tatatacact gatgtcattg
     1021 acagtatcat ataaaaatat tcggcggcat ccggctcttg agggattaac tgaaagagag
     1081 ccagttggca cagcatcagc gtcagggagc aaaaaaacac cctgctcctg ttgaaccacc
     1141 acccgaaaaa catagctgca gcaaacagta cataggaaac atactgcgaa agcagcttta
     1201 tggttggcgg agcagcttta agccatacgg aaacaaaata tgcgattata acaagcaggc
     1261 aattgactcc caaggatatc aaagcttttg tagttttatt cactttattt atcctcactc
     1321 tcgcttttct cttctgcaag gtgcatatag tattgataca actccgtata ttccgatact
     1381 tcctgccttc ctttatcgtt tattgcgtac aagcctttgg atatgttttc aaaccaccca
     1441 tagtgattct tttgaagtat ggctgttgtc tttttctcgt cggtgccaag ctgtctcagt
     1501 tccctgggct ttaattgacc gtatctcatc agacagcagg ctatatgtat tgccatttcc
     1561 ctatatgcag tcacaagctt ttcccttgtg ctccctccgg tgttaaaatc tccatatctt
     1621 gcggcaaact cctttactaa ctcctgcctt ttccttttgc tatgattctt gctcttagcc
     1681 atatcaaagg ccgcaggctc aaacacgact tcaacaaagg aacagccctc cttaagcgaa
     1741 acaaccataa ggccaagctg cagtcttttc aacatataac acagattctg ccatctcttg
     1801 gagaaaagcc ccctgcccgg tttaggcaca gcaatataaa ccatgtctgc aagcttttgt
     1861 cgctgaacag cctgaagaat gacatcgagg ttaagagctt ttttcatctc cactacaagc
     1921 agttcattct cttttatggc agcaatatcg cagtggttga cctcgctgcg cactgtatag
     1981 cccaagccgg caagatagtc ataaataggc ttgtacaaat ccttctcaaa aaattctttt
     2041 ttaccgctgc tcatgcaatc accacgcttt atatagaata tcgtcaataa accacattat
     2101 ttgaagaatg tatataaaga taccgataac actgaaggca cgaaaaccca cagaaatcac
     2161 ggcaaaaaaa tattcaggta tgttagtaat gatcgacaat agcaaacgta tcgagatcta
     2221 ttgatatatc gattaataac tatctagtac cgtgttattt cgtggaacag ccgtgtgttt
     2281 agtgttaccc gtcattttta cactatcggt gccagcagca gtacctcttc attctcccta
     2341 tatattgtat caaattgtga aatgggtatg ggattccgcc cgattcccac ctcaaaagta
     2401 tacccgggcc ttctgtactg ctccacaaac caatccttgt agcctgcgta agctgcttcg
     2461 taaggcacgc ccgaaactgc atatcctgtt atttccgaga aaatatttgc tatctccaat
     2521 gcccggggag gcctaatccc cttatacagc caatatatta cccttccttg ggtatgatag
     2581 gctataacca gcttaaaatt gtgctgtctt gtaaaattga cgacagcctg tgtctcaggc
     2641 tctgaaagag ggctcggccc tccatatctg gtaggccccg ggccgtctac acccagcgaa
     2701 ggctcctgtg cctttgcttc ctcccatgac gccggataat tgcggttcaa atcgactcct
     2761 ctgatattgg cattccaaac ctgtgagaag ggcaggcccg tatcattcca tgcaagcagc
     2821 cggtcataat agggattatc tcttttcaac ccctccaaca ctaggtttac tccatccgga
     2881 tttaccatag gcataatgta aatgctgcct ctgttccaca ggtcacgtat attatagccc
     2941 tggataccgg aattccttgc atatccccgg gcgtagtttt caataaattt cataagcagc
     3001 ggagccgtaa tccattcaag tgcatggtgt gcaccgttat agaacacttc tgaaggcccg
     3061 tttcccaatt ttatatagta taagtttttc ccaaggacgc ttcttcccgc gatacccact
     3121 tctataaagg ggtatctcac ctttagccct cttatgtctc tatccattat ctcatatgta
     3181 taatcgatat cggtaaaaac cacatcaatg ccataaggca cattgattac ctgcccgact
     3241 ctgaggttcc cgggatcgac tcccgggttg gctgtcatta cagcattcaa tgtagtgtaa
     3301 taccttccgg atatattgta aaacgtatcc cccggtcgga tagtataggt gtcataaccc
     3361 ctcaggaatc tctcaaacct gctccaggca gccgggcctg ccaccccgtc cggttcaagt
     3421 ccgttatcca acttaaattc ccttaccgct tcacgggtaa ccggtccaaa gataccgtcc
     3481 accggccccg ggttgtatcc gattcttatc aataggcttt gtataagctt tactgctgca
     3541 cctcttgagc ccaatgtcaa tacttccata aaaacgcctc ctgcaaagct agtgaacaat
     3601 accataatat tcattgcggg aggcaattgt tacatttata tgttattcag cttttgaacc
     3661 ggttgcacca tggcttttca ttaacttttt catcccatcc gtagcttcaa ccttcccatc
     3721 gggcataacc cacaccgcct caacgttgtc aaggctttcc acaagtttac ggcttttgtc
     3781 ataaggcagc agaaatgcag tagtggacaa aaaatccgca agtcccgcat cctcagtaac
     3841 tatcgtaact gcacggtagt attccccggg cattagcgta tccggatcaa tcaaatgatg
     3901 atagatcttg ccttcaacca cataatacct ttgataatcc ccgctgctta ctattgctgc
     3961 attattgaca aatatcgtat ccaacaggtt ctcttctgca gctataaaca ttttgtccgg
     4021 atcctgtata cctatacccc agtactcacg cattccgtca agaggctttc caagtacgcg
     4081 gatattaccc cctgggctta tgatgcccga agtaaaaccc tccgacataa tttccttggc
     4141 aaccagctcc gtggcaaagc cttttgctat ggctccgaca tcaaggcgca tttccttatc
     4201 agacagaaat accgtactat ttgcttcatc cactattact ttatttatgt cagtatgatt
     4261 gcttgcttct ctaagctttt ccaaaggagg cagctcggca ttcacgggat cgccctctcc
     4321 ccgctcgcga taatcatgcc atattgaaag caccgcaccc attgcaatat tggttcgccc
     4381 gcctgttttt gcatgccatt ccttcgcaaa caatatgaga tctgttattt ccttatccac
     4441 ctttacaggt ttgattcccg cattttcatt tattgtcttg atattattga taccttcgta
     4501 atcattatat atgtcataca gcttatgcag ctcttcaaaa cgagcgcgta tcttctcaaa
     4561 atactccgaa aattcctgct cgcttttgtt atagcccact acctgagtca gtgtatcaaa
     4621 agttccgaaa aaggtgtcac tgtatttagt taatactaat tccggagctt tcccgcatcc
     4681 tgataaaacc aaaagcagta taaccaatgt aattacaata acggaagttt tcttcattct
     4741 tatcaccctt aattttatta gcaaagctat tatagcaaat aatccatgtt ttatgcaaaa
     4801 aatccgtgaa gggacacggg gacgaaggga cacggggacg tttatcttgt cccgctgaaa
     4861 gcgggacaag ataaacgtcc ccgtgtccct tgtccccgtg tcccttaata tttatacgcc
     4921 ttattttgcg tttgcataac cttccgcaac tgctgcaata tattcctgta cagatacggt
     4981 aacagatgat gtaagctccg gaacatcagg aactgcagga tgtgatgcat ccttgttctt
     5041 tgtcttcata gccttgattt cgtcaacagt cttgccaacc atccactgtc caagggcatc
     5101 agcctgctga taccattctt tgcctatcga tgaagccttg atcattccat agccttcttt
     5161 tagctctacc tttgtcggat attcagctgc tttatctgca gttactttac cggtattgtc
     5221 aaaagtaatc ttagtttgtg cattatctat gatagttcca acaaccttgc cgtctttgtc
     5281 aaaggcagta gccgccatga ctgtatcaac ctgggcgaag ggaaggactt ctttgccttc
     5341 tgcatctttt ccaagcccct ttgatttacc tgttgttata acatgtccaa gtccaacctt
     5401 ttcaccgcct gctacatcga cagcagtttc ataagcttcc gcaacagctg caatgtattc
     5461 ctgtacagat acggtaacag atgatgtaag ctccggaaca tcaggaactg caggatgtga
     5521 tgcatccttg ttctttgtct tcatagcctt tatttcatca acagtcttgc caaccatcca
     5581 ttgtccaagg gcttcagcct gctgatacca ttctttgcct atcgatgaag ccttaatcat
     5641 tccatattct tctttaagct ctacctttgt cttgctttca gcctttatat ctgatgtaac
     5701 ctgaagagcc gcatcaaacg taaccttagt ctgtgcattg tcaatagtca ctgatataac
     5761 ttttccatcc ttgtcgaagc caacagcagc cataactgta tcaacctgtc ctaaaggaag
     5821 aacttctttg ccttcagcat ctttcccaag atcctttgac ttggcaattg tagtaatatg
     5881 tccaatgcct aatttaacaa tactgccgcc ttctgctgcg ggtgccgctg gttctgccgg
     5941 t
//
LOCUS       DAOOAB010000084         5751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_16129_length_5751_cov_14.506320, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000084 DAOOAB010000000
VERSION     DAOOAB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16129_length_5751_cov_14.506320"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /gene="sleB"
                     /locus_tag="PKU88_12005"
     CDS             complement(<1..632)
                     /gene="sleB"
                     /locus_tag="PKU88_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HPX70034.1"
                     /translation="MYSSYLTSQISSEAGAILLKWGSRGSEVREVQTRLKNWGFYKGS
                     VDGIYGWRTANAVKAFQRKHGLKADGIVGNATGKALGIRTGTASARGGTSRSNETYTL
                     AQCVHGEARGESYIGQVAVAAVILNRVDDPRFPKTIAGVIYQPGAFTAVTDGQMFQPP
                     GDTALKAARDALNGWDPSGGALYYYNPAKTTNKWIWSRPIIKVIGKHYFC"
     gene            complement(765..1436)
                     /gene="spoIIR"
                     /locus_tag="PKU88_12010"
     CDS             complement(765..1436)
                     /gene="spoIIR"
                     /locus_tag="PKU88_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HPX70035.1"
                     /translation="MKYKIGFFLIAFFFVFGILTGIYINTDMALADVPEKIIRLHVVA
                     NSDSPVDQQLKLQVRDKIISSMSGRLEGLKDISEVKSIVRGSLAEIEAAAREVIEENR
                     KLYGVKAAFTEMDFPTRAYGNITLPAGTYQALNIVIGEGKGKNWWCVMFPPLCFIDVA
                     HGVIAEETMKELRTSLTEEEYSLLISSKEPGEVSVKLRFKILELAKSMGLRLAKLGNL
                     QNISK"
     gene            complement(1700..2839)
                     /locus_tag="PKU88_12015"
     CDS             complement(1700..2839)
                     /locus_tag="PKU88_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HPX70036.1"
                     /translation="MRAIKSLKAAAVLLCVLLLAGCWDKIEIEDRLFVLSIGVDSAEA
                     AEQQVSEDKYTLSFVSPVVDQIKEGPGPAFHTYKTVDNSIIMSLSQLLERFSKKQFFG
                     HTRNIFFGEDILKDEKLLKEILDGISRFHEFHNSMYAYFVPGRAEEVFKVKPMYDKLL
                     APYIAGIIENSDYTSKIIKLTLSEMIIMLENQEGGLVIPKLNPGKEEVKINGAGVLKN
                     YKLIGYLGDQETSVYNWLTNKVRGGDIAIEHNNISTVFRHFTFDRKIELSKVENGNIY
                     LNYKMETEGSIEEYMIGKEIMDNALLKEISREVEKRIEDESEKLIKKFQQEFKADLIG
                     ARDYLSKYQPKLYKTIEKDYEKFFTDSMVIEVTANVNIRRVGLIK"
     gene            complement(2826..3935)
                     /locus_tag="PKU88_12020"
     CDS             complement(2826..3935)
                     /locus_tag="PKU88_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HPX70037.1"
                     /translation="MIRNNDTISPYQLAMITIMTIISVEVFSLAAEAADELGTDGWLV
                     IVILGIINIFAALVIIRLNSIFPGKTIAEYSQIIIGKIPGKVLTGLFAVYLIIVIAYV
                     TRVFTEVVKMFLLFRTPTEVIMLSLILVCTYAVRGGIECVARVNELIFPILFIPFFIV
                     LVFGFPIMDFSNLRPILQTPPDKILASIPRMIFSFGGIELALYYIGFMKEPGKAYKPL
                     IISVIFILLFFVMITVFCLAAFGAKAVTRIIWPLAAYIRTVSLPGVFLERLDGVILSL
                     WVLTVFTTIVSVLFIVSYSISKAAGTKEQKQFVLPLVIVIYYLALQPESLAELYEWGN
                     LIFPYAVAAFMYVVPAALLLTAKLRKMEVKRNESN"
     gene            complement(3922..5511)
                     /locus_tag="PKU88_12025"
     CDS             complement(3922..5511)
                     /locus_tag="PKU88_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HPX70038.1"
                     /translation="MGFFSKKKSGNVKKEKEMGKLLNSRNLDENTKRLKERLKDCDDI
                     VYKEFRVGEEQDLKFLLVYADGMSSRDMLNDAVLNALMVHAREVPPEAKEPGEELYRL
                     VRYGGLPASELADKDNLEDAILAILIGDAVLFIDTTEKCIVIGAKGWPMRGVSEPNTE
                     ALIRGPRDGFTETFRTNTALVRRRIRDSRLKLKQLRVGKRSFTDIGIMYIEDIASPEL
                     LEEVFRRIDTIDIDAIIESGYIEQLIEDNYMSPFPQISVTERPDEVAASLYEGRVAIL
                     VDNSPFALIVPTTLNSLFQSPEDYYDRWYVASILRFIRYMASILSLVLPASYIAVTSF
                     HPGILPAKLVISMAAARQGIPFPVLIEAFIMEVTFELLREAGLRLPVPIGSAIGIVGG
                     IVIGSAAVEAGIVSPIMVIIVAITAISSFSIPNYSLAVGFRMLRFLLMIFAGTMGLYG
                     IMLGVLLILSHLVKLKSFGIPYMSPYVSFVGSDFKDTIIRVPAFLMAKRPVLLARDKI
                     RLKNNRNLINRLEQEGSDDKK"
     gene            complement(5536..>5751)
                     /locus_tag="PKU88_12030"
     CDS             complement(5536..>5751)
                     /locus_tag="PKU88_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70039.1"
                     /translation="GYGMFEMIKENIKGFADAKVFMTMIAIGMFEILSDYRYFKRVKY
                     RKDSAVSLGLGIAFILLPFVFFFISKL"
BASE COUNT         1490 a         1462 c         1041 g         1758 t
ORIGIN      
        1 ttgcagaaat agtgcttccc aatcaccttg atgataggcc ttgaccaaat ccacttattg
       61 gttgtttttg ccggattgta gtaatacagc gctcctcccg aaggatccca tccgttcagt
      121 gcatctctcg ccgccttcag ggcggtgtct cccggaggct gaaacatctg gccgtcggtt
      181 acggcggtga atgcccccgg ctggtaaatt actcccgcaa ttgtctttgg aaacctcggg
      241 tcgtccactc tgttcaaaat aactgccgct actgccacct gccctatgta cgattctccc
      301 ctggcctcac cgtgcacaca ctgggcaagg gtataagttt cattgcttct ggatgtgcct
      361 cccctggctg atgcagttcc ggttcttata cccagggctt ttccggttgc attgcctact
      421 ataccgtctg ccttaagtcc gtgttttctt tgaaatgctt ttacggcatt tgctgttctc
      481 cagccgtata ttccatcaac gcttcctttg taaaaacccc aatttttcag ccttgtctgt
      541 acctctctca cctcactgcc tctggagccc catttcaata gtatggctcc cgcttcactg
      601 ctgatctggg aagtaagata gctgctgtac acccagaaac caaagcctgc atatatgatt
      661 actataacta taaaaggaat cagccttcct ggtctttttt tgaacactaa aaacacctcc
      721 tgcccttatt ttttgtaaca ggaggtgttt tattcatatt ttaattattt gcttatattc
      781 tggaggttcc ccagctttgc cagcctcagc cccatgcttt tcgccagttc aagtattttg
      841 aatctgagct taacggatac ttcccccggc tctttggagg aaatcagcag gctatattcc
      901 tcctctgtca atgatgtcct aagttctttc atggtttctt ccgctattac accatgggcg
      961 acatcaatga agcacagagg agggaacatg acacaccacc agttctttcc tttcccttct
     1021 ccgattacta tattcaaagc ctgatatgtg cctgccggaa gtgtaatgtt tccataggcc
     1081 cgggtaggaa aatccatctc tgtgaaggcg gcttttacac catagagctt cctgttctct
     1141 tctatgactt ctcttgcagc agcctctatt tcggccaggc ttccccgtac aatgctcttt
     1201 acctctgaaa tatccttcag cccttctagc ctcccactca tactgcttat tatcttgtcc
     1261 cttacctgaa gcttaagctg ctgatcaaca ggcgagtcac tgtttgctac cacatggagc
     1321 cttattattt tttcaggcac gtcggccaat gccatgtctg tatttatgta aatgcctgta
     1381 agaatgccga aaacaaagaa aaatgcaatc aggaaaaatc cgattttgta tttcatactc
     1441 acccctccaa tttatattaa ctaaaacacg ataacgcagg taacactaaa cacacggatt
     1501 tcccacggaa tacacggcga taatattata tttttctgta tcgctgcttt ggtaaatatc
     1561 gatactccct aaattatcaa ttacccaata acatatctac gtcatttttt aattatccgt
     1621 gccttcagtg gattttagcg tttttggtgt tattcgttcc cctgtgcctg ttatttttgc
     1681 catgttttga agggtttatt cacttgatta atcctactct ccttatattg acattggcgg
     1741 tgacttcaat taccatactg tcggtaaaaa acttttcata gtccttttct attgttttgt
     1801 aaagcttcgg ctgatatttg ctcaggtagt cccttgctcc gataagatct gctttgaatt
     1861 cctgctgaaa tttttttatc agcttctcac tttcgtcctc tatccttttt tccacttccc
     1921 tgctgatttc cttaagcaaa gcgttatcca ttatttcctt tcctatcata tactcttcaa
     1981 tactgccttc agtttccatt ttgtagttca gatatatatt cccattttcc accttggaca
     2041 gctcaatttt cctgtcaaag gtaaagtgcc tgaaaaccgt ggaaatatta ttatgctcaa
     2101 ttgctatgtc ccctcccctg accttgtttg tcagccaatt atatactgat gtttcctgat
     2161 cgcccaaata tccgataagc ttataattct tgagaacccc cgcaccattt atctttacct
     2221 cttccttccc cggattaagc tttggtatga caaggcctcc ttcctggttt tccagcatta
     2281 ttatcatctc tgagagagta agcttaataa tctttgaagt atagtcacta ttttctatta
     2341 ttcccgcaat gtacggtgca agtaatttgt catacatagg cttgacctta aatacttcct
     2401 cagcccgccc cggtacaaaa tatgcgtaca tgctgttatg aaactcatgg aacctggata
     2461 tgccatccaa tatttcttta agcagcttct catctttcaa aatatcttca ccaaaaaata
     2521 tattcctggt atggccgaaa aactgcttct tggaaaacct ctctaaaagc tgcgacaagg
     2581 acataataat gctgttatcc actgttttgt atgtatgaaa ggcaggcccc ggcccttctt
     2641 ttatctgatc taccaccggg gaaacaaagg aaagagtata tttatcctca gaaacttgct
     2701 gctccgctgc ctctgcacta tctactccaa tagataatac aaataacctg tcttctatct
     2761 ctattttatc ccagcagccg gcaagcagaa gcacgcacag cagcaccgct gccgctttca
     2821 agcttttaat tgctctcatt ccgtttcacc tccatcttcc ttagcttggc tgtcaaaagt
     2881 aatgcagcgg gcactacata cataaaggcg gcaacagcat agggaaaaat taagttgccc
     2941 cattcataaa gctcagctaa gctttcgggc tgcagtgcaa gatagtatat aactattacc
     3001 aaaggcagca caaattgctt ttgctccttt gtacctgccg ccttagatat ggaatagctt
     3061 acaatgaaaa gcaccgatac gatggtagtg aataccgtca gcacccaaag ggacagtatt
     3121 actccgtcaa gcctttctaa aaacactccg ggaagactga ctgtccttat gtaagcagcc
     3181 aaaggccata ttattcttgt taccgccttt gctccgaaag cagctaagca aaagactgta
     3241 atcattacaa agaataataa tatgaaaata acagagatta ttaaaggttt atatgccttc
     3301 ccgggctcct tcataaaacc tatataataa agggcaagct ctatgccgcc aaagctgaat
     3361 atcatccttg gaattgaagc caatatttta tccggaggtg tttgcaggat tggacgcaaa
     3421 ttgctgaaat ccataatagg aaacccgaat accagtacaa tgaaaaatgg tatgaagagg
     3481 atagggaata tcagctcatt tacccttgca acgcactcga tacctcctct tactgcatat
     3541 gtgcaaacca aaataaggct aagcatgatt acttcggtag gagtcctgaa taacaggaac
     3601 atctttacta cctcggtaaa aaccctggtg acatatgcta tgacaatgat aaggtaaacg
     3661 gcaaaaagac ccgtcaatac ctttcccgga atttttccta taattatctg gctgtattct
     3721 gcaatggtct tgccggggaa tatggaatta agcctgataa taaccaatgc ggcaaaaatg
     3781 tttattatcc ccaagattac aattaccagc cagccgtcgg tccccagctc atctgcagct
     3841 tctgccgcaa gggagaatac ctctacagaa attatagtca taatagtaat catcgcaagc
     3901 tggtatggag atatggtgtc attatttctt atcatcactg ccctcctgtt ccagccggtt
     3961 tataaggttc ctgttgttct tcagccttat tttgtccctg gcaagcagta caggcctttt
     4021 tgccataagg aatgcaggta cccttataat tgtatccttg aagtcgcttc ccacaaagga
     4081 tacatagggc gacatataag gtattccaaa gctcttaagc tttaccagat ggcttaatat
     4141 caaaagtact cccagcatga tcccatataa ccccattgtt ccggcaaaaa tcatcagcaa
     4201 aaaccgcagc attctgaacc ctactgcaag gctgtagttg ggaattgaga aggaagatat
     4261 cgcagttata gccacgatga taaccataat cgggcttact attccggctt caactgccgc
     4321 agagcctatt acaatacctc ccactatacc tatggcgctg cctatgggta ccgggagcct
     4381 aaggcccgct tctcttaaga gctcaaaggt aacttccatg ataaaggcct caatcagcac
     4441 cggaaacgga atcccctgcc gagccgccgc cattgatatt acaagttttg caggcagaat
     4501 gcccggatgg aaggaagtaa cggctatata tgatgcggga agcaccaggg aaagaatact
     4561 tgccatgtat cttatgaatc tcaatatgga tgcaacatac cacctgtcat agtaatcctc
     4621 gggagactgg aacaagctgt tcagagttgt aggtacaatc agggcaaaag gcgaattgtc
     4681 aacaagtatt gccaccctgc cctcatacag tgatgctgca acttcatccg gcctttcggt
     4741 tacacttatc tgagggaagg gcgacatata attatcctca atcagctgtt ctatataacc
     4801 gctctctatt attgcatcta tatctatggt atcaattctg cggaatactt cttccagaag
     4861 ctccggactt gctatatctt caatgtacat gatccctata tccgtaaagc ttctcttgcc
     4921 taccctcagc tgtttcagct tcagccttga gtcccttatc cttcttctta ccaatgcggt
     4981 atttgttctg aaggtttctg taaagccgtc tctgggtcct cttatcagtg cctcggtgtt
     5041 aggctccgat actcctctca ttggccagcc tttagcgcct ataactatgc acttttcggt
     5101 agtatctatg aataacactg cgtctccgat aagtatagcc agtattgcgt cttccagatt
     5161 gtctttgtca gcaagctcag aagccggaag ccctccgtac cttaccagcc tgtacagctc
     5221 ttcccccggt tctttcgctt cgggaggcac ttctctggca tgtaccatca aggcgttgag
     5281 cactgcatca ttaagcatgt ccctgctgct cattccgtct gcatatacaa gcaggaattt
     5341 tagatcctgc tcctccccta ccctgaattc tttgtatact atatcatcac agtccttcag
     5401 gcgctccttc agtctttttg tattctcatc caggttcctt gaattgagca gttttcccat
     5461 ttccttttct ttcttgacat taccgctctt cttctttgaa aaaaagccca tcggaattcc
     5521 tcccttctgc ggctgctaca gcttgcttat aaagaaaaat acaaagggca gaagtatgaa
     5581 tgctatcccg agccctaagg aaaccgccga gtcctttcgg tattttaccc gcttgaaata
     5641 cctgtagtcc gatagtatct caaacatgcc tattgctatc attgtcataa atactttggc
     5701 atccgcaaag cccttgatgt tttccttgat catctcgaac attccatatc c
//
LOCUS       DAOOAB010000085         5290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_17864_length_5290_cov_15.520344, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000085 DAOOAB010000000
VERSION     DAOOAB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17864_length_5290_cov_15.520344"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKU88_12035"
     CDS             <1..219
                     /locus_tag="PKU88_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70040.1"
                     /translation="GSAGAAGAEHPASTESANVSNKTIDNTFFMFPPPNCLLNIYYYF
                     ILSYCINIINVYRLILFINYDNLYRWCN"
     gene            complement(286..723)
                     /gene="mscL"
                     /locus_tag="PKU88_12040"
     CDS             complement(286..723)
                     /gene="mscL"
                     /locus_tag="PKU88_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPX70041.1"
                     /translation="MWKEFKAFAMKGNVIDLAVGVIIGGAFGKIVSSLVNDIIMPFVG
                     LLIGKLDFANLFITLGDGSFNTLAEAKEAGVATLNYGLFINNIIDFLIVAFSIFLVIK
                     QINRIPRKKEEPAPVQTKSCKYCFSEIHIDASKCPHCTSDLND"
     gene            complement(942..1565)
                     /gene="thiE"
                     /locus_tag="PKU88_12045"
     CDS             complement(942..1565)
                     /gene="thiE"
                     /locus_tag="PKU88_12045"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPX70042.1"
                     /translation="MPDKGLVNYSLYLITDRKVLGGKELLDCIEDAIKGGVTLVQLRE
                     KNINTFDYYNLALKVKELVRKYGIPLIINDRVDIALAVDAEGVHLGPEDLPIAAARKL
                     MGRDKIIGASANCIEEALDFQRQGADYLGIGALFPTKTKGNTEHVTLTQLKAIKEAVH
                     IPIVGIGGISTENAASVKAAGADGIAAASGILGSENIVEAARRLKGL"
     gene            complement(1555..2367)
                     /gene="thiM"
                     /locus_tag="PKU88_12050"
     CDS             complement(1555..2367)
                     /gene="thiM"
                     /locus_tag="PKU88_12050"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPX70043.1"
                     /translation="MDIIGEASELLIRIKEYRPLIHHITNYVTMNDCANAVLAIGGSP
                     IMAKDPSEVEEVAAMSRALVLNMGTPDNNNFRSMLLAGKKAKEMRIPVIFDPVGAGAT
                     SFRREICCKLLSDIKPDVIRGNISEIMYLAGKDRYSCGVDSNWDIDNAVGIVQGLAKD
                     LNCVVAATGRKDLVSDGSTAYIINNGHEMLSKITGAGCMTSSIVGTFCSMTEDYLTGA
                     VGGVMVMGISGQLANACLMPNEGIGMFKVRLFDSMYNLNRDIFMREGVVYAG"
     gene            complement(2334..3560)
                     /gene="cytX"
                     /locus_tag="PKU88_12055"
     CDS             complement(2334..3560)
                     /gene="cytX"
                     /locus_tag="PKU88_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HPX70044.1"
                     /translation="MNKSNNNLTLLSFAALWFGAAVSIAEILTGGLLAPLGFKNGIIA
                     IIIGHIIGTLILVAGGIIGTETGLPSIASTGISFGKYGAYVFSVLNVLQLLGWTAVMI
                     ISGARSVNEIAKVLWSFDNIYLWSVVIGGLICLWIFFGNVGVKKLNLIAVVLLFILTV
                     MLSAVVFKNSELFSSSGDGTLSFGSAIELNVIMPLSWLPLIADYTRFAKRKGDAVTGS
                     FLGYFTGSTWMYTIGLGAAIASGNSDPGAMMLSANLGFTALGIVILSTVTTTFLDANS
                     AGVTFLNLFPKLNERKTALGMGAAGTLIAVIVPIEQYQNFLYAIGSVFAPLFAILLTE
                     YFVLKKSRKLQEGLLVNWGASAVWVAGVIMYYQFIKLDFVIGATVPVMIITGLLYCLS
                     WRWTESWILLEKHRNC"
     gene            complement(3553..4359)
                     /gene="thiD"
                     /locus_tag="PKU88_12060"
     CDS             complement(3553..4359)
                     /gene="thiD"
                     /locus_tag="PKU88_12060"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPX70045.1"
                     /translation="MKNVLTIAGSDSCGGAGIQADLKTFSAHGVYGMSVITAITAQNT
                     QGVLAVQDISVELIEKQIEAIFEDIRVDGLKIGMVSQIDTINAIGEELRYYKPSNIVL
                     DPVMVSKSGYHLLQQSAKEALIKSLVPLATIVTPNIPEAEVLTGMRIGSIEEMEKAAR
                     VIFEMGPRNVLLKGGHLDEDATDILFDGSKISCLKGKRINTKNTHGTGCTLSAAIASN
                     LAKGYSVAEAVALAKEYITIAIENSLSIGKGVGPTNHFYTLYKKAGMLDE"
     gene            complement(4374..4865)
                     /gene="thiW"
                     /locus_tag="PKU88_12065"
     CDS             complement(4374..4865)
                     /gene="thiW"
                     /locus_tag="PKU88_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HPX70046.1"
                     /translation="MQTQKLTFSALLVAIGTISANLVYIPVGAAKCFPVQHTINVLSA
                     VLLGPWYGVANAFLISLLRNILGTGSLLAFPGSMIGAFIAGIMYLKFGRKLFAAAGEI
                     FGTGILGGLLAFPIAKFLMGNDAAAFYFVVPFLISTIGGSIIGYVILGVMEKSHALKK
                     TGI"
     regulatory      complement(4986..5094)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1463 a         1356 c          921 g         1550 t
ORIGIN      
        1 ggttctgccg gtgctgcagg tgctgaacat ccggccagta ctgaaagtgc taatgtcagt
       61 aataaaacga ttgataatac ttttttcatg tttcctcctc caaattgttt gttaaatatt
      121 tattactact tcattctatc atattgtata aacattatca atgtttatcg attaatatta
      181 tttataaatt atgataacct ttatcgctgg tgcaattaaa tcagctgata aaaaagaaac
      241 agcatgcaga gtattatcta catgctgttc ctatactaat atgaatcagt cattcaaatc
      301 tgaagtacag tggggacatt tacttgcatc aatatgtatt tcactgaaac agtacttgca
      361 agactttgtt tgaacagggg caggttcttc cttcttcctt ggaatacggt ttatttgctt
      421 tatcactaaa aatattgaaa aagcaactat cagaaagtca atgatattgt tgatgaataa
      481 tccgtaattc aaggtagcga cacccgcttc cttggcttcc gccagggtgt tgaagcttcc
      541 gtcgcccagg gttatgaata aattggcaaa atccagcttt ccaatcagca agccaacaaa
      601 aggcattatt atgtcattga ccaatgaaga gactattttc ccgaaggctc cgccgattat
      661 aacaccaacc gccaaatcta ttacattccc tttcattgca aatgccttaa actctttcca
      721 catagaaatc ctccttttcg catatgaaga ataatgcgac gaggacatgg ggacgcgggg
      781 acatggggac gtttcccctg tcccacttcc agtgggacac gcgaaacgtc cccgtgtccc
      841 cgtgtccccg tcgtattctt catatattgt gcaacagttc tatcttttat ttgattgtat
      901 attcattata acggtatatt cgacatattt caaagaaaat attataaccc cttaaggcgg
      961 cgagcagctt caactatatt ctcacttccc aggattcctg aggccgccgc tattccgtca
     1021 gctcccgctg ctttgactga agcggcgttt tcagtgctta tcccaccaat gcccactatg
     1081 ggtatatgta ctgcttcctt tattgccttc aattgggtca gtgttacatg ctcggtattg
     1141 ccttttgttt tggtcgggaa caatgcacca atccccagat agtctgctcc ttgtctctga
     1201 aaatcaagtg cttcttcaat gcaattggca gaggcaccta ttatcttatc gcgccccatt
     1261 agttttctgg cagccgctat gggcaaatcc tcagggccca gatgcactcc ctccgcatcc
     1321 accgccaatg ctatatcgac cctatcgtta attataagag gaatgccgta tttccttacc
     1381 agctctttaa cctttaaagc caggttataa taatcaaaag tatttatatt cttttcccta
     1441 agctgcacta atgtcactcc gccctttatt gcatcctcaa tgcagtcaag cagttccttc
     1501 cctccaagta cttttctgtc ggttatcaga tataaagaat agttcaccaa ccctttatcc
     1561 ggcatatacc acaccttctc tcataaatat atccctgttc aaattataca tactgtcaaa
     1621 aagccttacc ttaaacattc ctatgccttc attgggcatt aaacatgcat ttgccagctg
     1681 tcctgaaatt cccataacca ttacgcctcc cacagccccc gtcaaatagt cctccgtcat
     1741 actgcagaaa gtgccgacga tggatgaagt catgcatccc gctccggtga tttttgaaag
     1801 catctcatgt ccgttattta ttatataagc cgtacttccg tctgaaacca ggtctttcct
     1861 tcctgtggca gcaacaacgc aatttaaatc cttcgccaag ccctgaacta tccccacagc
     1921 attatcaata tcccaattgg agtctactcc gcaagaataa cggtctttgc ctgccaaata
     1981 cataatttcc gaaatgttcc ctctaattac atcaggcttg atatccgaca gcagcttaca
     2041 gcagatctct ctgcggaaag aagtagctcc ggcaccaacc ggatcaaaaa ttacaggaat
     2101 cctcatttct tttgcctttt tgcctgccag cagcattgat ctgaaattat tattgtccgg
     2161 cgtccccata ttgagcacca atgctcttga cattgccgcc acctcttcaa cctctgaggg
     2221 gtcctttgcc attatgggag agcccccgat cgcaagtacg gcattggcac aatcattcat
     2281 agtaacatag ttggtaatat gatgaattaa aggcctgtat tcttttatcc ttatcagcag
     2341 ttccgatgct tctccaataa tatccaactc tctgtccacc tccatgacaa gcaatataac
     2401 aatccggtga ttatcattac gggaactgtc gccccaatta caaaatcaag cttgatgaac
     2461 tggtaataca taattacacc cgcaacccat actgctgacg ctccccagtt tactagcagc
     2521 ccctcctgaa gcttcctgct tttctttaaa acaaagtatt ccgtaagcaa tattgcaaaa
     2581 agcggtgcaa aaaccgaccc tattgcatac aggaaattct gatattgttc tatcggtact
     2641 attactgcaa tcaaagtccc tgctgctccc atgcctaatg cagtctttct ttcgttcagc
     2701 tttgggaata aattcaaaaa tgtcactcct gccgaatttg catcaagaaa ggtagtcgtt
     2761 acagttgaca gtatcacaat gccaagggca gtgaacccta agtttgccga tagcatcatt
     2821 gcccccggat ctgaatttcc cgatgcgata gcagcaccaa gaccaattgt gtacatccat
     2881 gtacttcccg taaaatatcc cagaaagctt cctgtaacag catctccttt tctttttgca
     2941 aaccttgtat aatctgctat caggggaagc catgataaag gcattatcac attcagctct
     3001 atggcagacc cgaaggatag ggtgccgtcc cccgagctgc tgaataattc actattctta
     3061 aaaacaaccg cactcagcat tactgtaagt ataaaaagca gcactacggc aatcaggtta
     3121 agctttttca caccgacatt tccaaaaaat atccacaaac aaatcagccc gcctattacg
     3181 acactccaga gataaatatt gtcaaaggac cacaatactt ttgcaatttc attgaccgat
     3241 cttgcccctg atattatcat gactgcagtc cacccaagga gctgaagcac attcagtacc
     3301 gaaaaaacat aagctccgta tttgccgaag gatattcctg ttgacgctat tgaaggcagc
     3361 cctgtttctg ttccgattat cccccctgcc actaaaatca aagtgcctat tatatgtccg
     3421 attattattg caattatacc attcttaaag ccaagggggg caagcaatcc tccggtcagt
     3481 atctcagcaa ttgatacggc tgccccgaac cagagagcgg cgaagcttaa tagcgtaaga
     3541 ttattgtttg atttattcat ccagcatacc cgctttcttg tataacgtgt aaaaatgatt
     3601 tgtaggccct acccccttgc ctatggacag ggaattttcg attgcaattg taatgtactc
     3661 ctttgccaat gcaacagctt ctgcaacaga ataccctttt gccaggttgg aggcaatagc
     3721 cgccgaaagg gtgcagcctg ttccgtgagt attcttagtg ttaatcctct tgcctttcag
     3781 gcagctaatt ttactgccgt caaaaagaat atccgttgca tcctcatcaa gatggcctcc
     3841 ctttaaaagt acgttacggg gacccatttc aaaaatcacc cttgctgctt tttccatttc
     3901 ttcaatactt ccgattctca ttccggtaag cacttccgct tcgggaatgt taggcgtaac
     3961 aattgttgcc aggggcacca aacttttgat caatgcttcc tttgccgact gctgcaatag
     4021 atgatacccg cttttggaaa ccataaccgg atccagtaca atatttgaag gcttatagta
     4081 acgcagttct tcccctatgg catttatcgt gtctatctgt gagaccattc cgatttttaa
     4141 accgtcaacc ctaatatcct caaagattgc ttctatttgc ttctctatca attcaactga
     4201 aatatcctga actgccagga ccccttgtgt gttctgcgcg gttatggcag taataacact
     4261 catcccgtaa accccatggg cggaaaaggt tttcaggtcc gcctggattc ctgcgccccc
     4321 gcagctgtcc gagcctgcta tggtcaaaac gtttttcata tcaattcctc ccgctaaatg
     4381 cctgttttct tcagtgcatg gcttttttcc attactccca gaattacata gccaattata
     4441 ctgccgccaa tagtgcttat caggaaaggt actacgaagt aaaaggcggc cgcatcattc
     4501 cccatcaaaa attttgctat gggaaaagcc aaaagtccgc ccaatatgcc cgtaccgaat
     4561 atttctccgg ctgcggcaaa taacttcctg ccgaatttca aatacataat ccctgctata
     4621 aaagctccaa tcatgctccc ggggaaagcc aaaagggaac cggtgcccaa tatgtttctc
     4681 agcagcgaaa tcagaaatgc attggccacg ccataccacg ggcccaacag cacagcagac
     4741 agtacattta ttgtatgctg caccgggaag catttggctg ctcccacggg tatgtagacc
     4801 aaattggccg atattgtgcc tattgctaca agcaatgcag aaaatgtaag tttttgggtt
     4861 tgcataaaaa atcctccttt atcggcaggg ggcttctccc ccttgcatcc cccatcgtat
     4921 aggaaattat aattttctat acgataaaaa agcataaacc ccattataag gatttatgct
     4981 tatattctat aaacatgtta cttccctacg ctggcattat ccagatcagg taatagggtc
     5041 agagactcat gttctctatc tcagccggcc ttaccagccc ccccagtact taagctattc
     5101 aattaccata atagtaacac atactattgg ccatttcaag tctgaacgtt aaaactgccc
     5161 atcagcatct tttctgaatt tatcaacttc atcggataac ttgtaatccg atttttccag
     5221 gacctccata tacttgccca aaagctgcaa cagctcacta tctccttttt ccttcaaaac
     5281 ctttgtataa
//
LOCUS       DAOOAB010000086         4939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_19405_length_4939_cov_14.798935, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000086 DAOOAB010000000
VERSION     DAOOAB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19405_length_4939_cov_14.798935"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..265)
                     /locus_tag="PKU88_12070"
     CDS             complement(26..265)
                     /locus_tag="PKU88_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPX70047.1"
                     /translation="MRLDKYLKNSRLIKRRTVAKEACEGEKVYLNGKLAKPSAEVKVG
                     DIIEIVFGEKRIKAEITDISDNVSKASAREMYKLI"
     gene            complement(351..632)
                     /locus_tag="PKU88_12075"
     CDS             complement(351..632)
                     /locus_tag="PKU88_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPX70048.1"
                     /translation="MNKSDLISKMAEKSAMTKKDSEKALKALVESVEEALAKGDKVQL
                     VGFGTFEVKARKARVGRNPRTREEIKIPASKAPIFKAGKDLKDLVNKKK"
     gene            complement(717..2201)
                     /gene="mazG"
                     /locus_tag="PKU88_12080"
     CDS             complement(717..2201)
                     /gene="mazG"
                     /locus_tag="PKU88_12080"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPX70049.1"
                     /translation="MKEITIVGLGPGDYKQLSLEALEIMKNADILVFRTEKHPVAERI
                     RESGISFTCCDDIYDSCESFDETYGQISRRILDLCREKGQVVYAVPGHPLVAEKSVEL
                     ILAGAGKDIKVSIVPALSFIDVILSSLKIDPIYGLKIIDGLSIKTQKPDKKCGNIITQ
                     VYDKLVASEVKLALMDYYTDDFEIYIIRAAGVEGQERIASIPLYELDRIEWMDYLTSI
                     YVPPALDSGARYKSFEDLVELMENLRGENGCPWDKEQTRESLKPYLLEETYEVLDAIE
                     RDDLELIVEELGDLLLQVVFHSQIAREDGEFDINEVITNIVNKLIIRHPHVFGDIKAT
                     SESGALKSWEASKRKYKGIDTYTKTLEDIPDILPALMRSYKVQQKAALAGFDWDEVEE
                     AFSKVYEEAEELKEVYKTGEKAIIEEEIGDLLFSVVNVSRFLKVQPELALRSTTEKFI
                     QRFKYIEETALQSGRKLDKMSLQEMDMLWNEAKQQVLAKKDEKR"
     gene            complement(2314..3915)
                     /locus_tag="PKU88_12085"
     CDS             complement(2314..3915)
                     /locus_tag="PKU88_12085"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPX70050.1"
                     /translation="MGYRKQSFIKGAAILAAAGIISKALGAIYRIPLGRIIGSEGMAY
                     YQTAYDLYIMMLTISAYSIPIAISKLVSEKIETGRRDEAHKTFKVALSLIAFLGITLS
                     AILFFNAKGFATMVKNPGAYVAVLCVAPAIFTVALSGAFRGYFQGMQNMTPSGLSQVT
                     EQIARVVFGFILAITFLPMGYESAAGGAVFGTTIGGLVSFLTLIYIYRMKRGEIKAGI
                     RADLQSKKESALSLVKRIVVFSVPITIGGSIMPVMNVIDTFIVMDRLQAAGFSRETAT
                     SLLGQLKGMAGAFINLPQVFTIALAASLVPAISESIAKSDFDGASRKSELAIRLSLLI
                     GLPAAFGLFTLADPIMKLMYPAEPATLGMVLRYLSTAVIFLTLVQTMTGILQGMGKER
                     IPVISLIIGALVKFAVSYTLTSIPVINIRGAAIGTVAGYAATAFINLSAIVKHQKSSM
                     RISKIVVKPVIATGAMMAAAYLSYRMLFGAIGRNSVATLISVAFAALVYGVALILIKG
                     IATEELEMAPGGKRLSSILKKKGLL"
     gene            complement(4173..4730)
                     /gene="spoVT"
                     /locus_tag="PKU88_12090"
     CDS             complement(4173..4730)
                     /gene="spoVT"
                     /locus_tag="PKU88_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HPX70051.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEV
                     ILKKYSPIGELSDFAKEYVESLHSSMEHITCIADRDTIIAVAGAPKKELLDKRVSPSL
                     ERIMEDKTTVVLSLTGENKMINISADEEGTPKYTAGVVAPIITQGDSIGAVLLLSKDP
                     NTQMGELEVKLSETAASFLGKQMEQ"
BASE COUNT         1276 a         1211 c          815 g         1637 t
ORIGIN      
        1 agaagtgcgc ccttcggcgc acttcttata taagcttata catctctctt gctgaagctt
       61 ttgatacatt atccgatatg tctgtaatct ccgcctttat tcttttttct ccgaagacaa
      121 tctctattat gtctcccacc ttcacttcag cacttggctt tgccagtttg ccattcagat
      181 agaccttttc accttcacag gcctccttgg ctaccgtcct tctttttatc agcctggaat
      241 tctttaagta tttatcaagt ctcaatcagt tcacatcctc tttataacat agaaatagta
      301 tggaaatgaa aaatcaggcc cgtccggcct gattcctcta aaatatgtag ttattttttc
      361 ttatttacga gatccttaag atccttgcca gctttgaata tcggagcttt tgatgctggt
      421 attttgatct cttctctggt tcttggattt ctgcctactc tagcctttct tgctttaact
      481 tcaaaagttc caaagccgac taactgaact ttatcacctt ttgcaagtgc ttcttcaaca
      541 ctctcaacga gtgccttaag agccttttcg gaatctttct ttgtcatagc acttttttca
      601 gccattttgc tgataagatc tgatttattc atcattttcc ctccattttt tattttgtag
      661 gaaatattta ctgtttatgg atattctacc tcattcccga aaatccttct tttttttcat
      721 cttttttcat ctttttttgc taaaacttgc tgttttgctt cgttccacag catatccatc
      781 tcctgcaaac tcattttatc aagttttctg ccggattgca aggctgtttc ttcaatatat
      841 ttgaaccttt ggataaattt ttccgttgtg cttcttaggg caagctcagg ctgaaccttt
      901 aagaatcttg atacattaac aactgagaac agcaaatccc ctatttcttc ctctattata
      961 gccttttctc cggttttata tacttccttt aactcttctg cctcctcata aacctttgaa
     1021 aatgcttctt ctacttcatc ccagtcaaag cctgccaacg ccgctttctg ctgaaccttg
     1081 tacgatctca ttaatgccgg cagtatgtca ggtatgtctt caagagtctt tgtatatgtg
     1141 tctatgcctt tatactttcg ttttgatgct tcccaactct ttaacgctcc gctttcactg
     1201 gtagctttta tatctccaaa cacgtgagga tgccttatta tcagcttatt tactatgttg
     1261 gttattactt cgtttatatc aaactctccg tcctccctgg ctatttgtga atgaaataca
     1321 acctgcagca gcaaatcccc aagttcttca actatgagtt caaggtcgtc cctttcgata
     1381 gcatccagaa cctcataggt ttcttcgagg aggtacggct ttaaggactc tcttgtctgc
     1441 tccttatccc aggggcagcc attctcgcct ctcaagtttt ccataagctc caccaaatcc
     1501 tcaaagcttt tgtatcttgc tcccgaatcc aatgcgggag gaacatagat gcttgtaaga
     1561 taatccatcc attctatcct gtccagctca taaagcggta tgctggcaat gcgctcctgt
     1621 ccttccactc cggctgccct tattatatat atttcaaaat cgtccgtgta gtaatccatc
     1681 agcgccagtt ttacttctga agcaaccagc ttgtcatata cctgggttat aatatttccg
     1741 catttcttgt cgggcttctg ggttttgatg ctcaacccgt caatgatttt caaaccgtat
     1801 atgggatcta ttttcaggga gcttaatata acatcaatga agctcaatgc aggaactata
     1861 cttactttaa tgtccttccc cgcaccggcc aggataagtt cgacactttt ttccgccacc
     1921 aggggatgtc ccggcacggc atatactact tggccctttt cgcggcacaa gtccaatatg
     1981 cgccggctta tctgcccgta tgtctcatca aaggattcgc aactgtcata tatatcatcg
     2041 cagcatgtaa agctgatgcc tgattctctt atacgttccg caacaggatg tttttctgtc
     2101 ctgaagacca atatatcagc gtttttcatt atttcgaggg cttccaggct aagctgctta
     2161 taatcccccg gtccaagccc aacaatagtt atctctttca tatatcttgt ttctccctat
     2221 ctttagttta tcttatttca gttccggaac cccgtatcct caaagccccg aagccctgcc
     2281 cacaatctgg catctggtat ctggcgtctg aatttataat aacccttttt tcttcaatat
     2341 gcttgaaagt cttttgcccc ccggagccat ttccagttct tccgttgcta ttccttttat
     2401 aagtatcagt gcaaccccat ataccaatgc agcaaacgcc acgcttatca gcgttgccac
     2461 agaattcctc cctattgcgc cgaaaagcat cctataactc aaatatgccg cagccatcat
     2521 ggcaccggta gctattacag gcttcacgac tattttggat atcctcatac ttgacttttg
     2581 gtgttttact attgcagaaa ggttaataaa ggctgtcgcc gcatatcctg caacagtacc
     2641 tattgctgca cctcttatat ttataacagg tatggaggtc aaggtataac ttactgcaaa
     2701 tttaaccaaa gccccaataa tcaggcttat tacaggtatc ctttccttcc ccataccctg
     2761 aagtattcct gtcatagtct ggacaagggt caaaaatatg actgctgtag aaagataacg
     2821 aagtaccatt cccaaggttg ccggttctgc cggatacata agcttcatta ttggatctgc
     2881 aagtgtgaaa agtccgaatg ccgcaggcag cccaattaat aaactaagcc ggattgccag
     2941 ttctgacttc ctggaagcgc catcgaagtc gcttttggct attgactccg atatagcagg
     3001 aaccaggctt gcggctaaag ctatggtaaa tacctgaggc aaattaataa aagcccctgc
     3061 catgccctta agctgcccaa gcaggctggt tgcagtctca cgggaaaaac ctgctgcctg
     3121 aagtctatcc ataactataa aggtatcaat aacattcatt accggcatta tactaccgcc
     3181 aatggtaatc ggtacggaaa ataccactat tctctttaca aggcttaaag ctgactcttt
     3241 ctttgattga aggtcagccc ttattccggc ttttatctct cctcttttca ttctgtatat
     3301 ataaatgaga gttagaaaac tgacaagccc tcctatggta gtgccaaata ccgcccctcc
     3361 tgctgcagat tcatatccca taggtaagaa tgttatcgcc aatatgaaac cgaaaacaac
     3421 acgggcaatt tgttctgtca cctgtgataa tccactggga gtcatattct gcatgccctg
     3481 aaaataccct ctgaaagctc cgctcagcgc tactgtgaat attgccggag caacacagag
     3541 aacagctacg taggcccctg gatttttcac catagtcgca aagcctttag cattgaagaa
     3601 caatatcgca gatagagtta tgcccaagaa ggctatcagt gaaagtgcaa ctttaaaggt
     3661 cttgtgagct tcatcccgcc ttcctgtctc tattttctcc gatactaatt tggatatggc
     3721 tataggtatg gagtatgctg aaattgtcag catcattatg taaagatcgt atgcagtctg
     3781 ataatacgcc attccctcag agcctattat cctgcctaaa ggtattctgt atattgcgcc
     3841 cagtgccttt gatattatac ctgcagctgc aagaattgca gcacctttaa taaaggattg
     3901 cttgcgatag cccatttttg acctcccaaa aaaacttaca tataatatac cgataaaaag
     3961 catattaaat agcgtaaacc tttgaagttc tttataaatc aatgataaat ctttttcgca
     4021 acatatatat cctaaatgta tagcattata attttaacat atatgcacat aactaacaaa
     4081 agtatttatc tataattaat atagcctaaa atcaagcata atagaacaaa aaagtagcag
     4141 cctattcgac tactaccttg ttatatacta aattactgct ccatctgctt accgaggaag
     4201 ctggctgccg tttccgacaa ttttacctcc agttctccca tctgtgtgtt tggatcttta
     4261 gaaagtaaca aaactgcgcc tatagaatca ccctgggtta ttatcggcgc tacaactccg
     4321 gcggtatact tgggggtacc ctcttcatca gcagatatat tgatcatttt gttttctccg
     4381 gtcaggctta aaacaactgt tgtcttgtct tccattatcc tttccaatga agggcttacc
     4441 ctcttatcca gcagctcttt cttcggcgct cccgcaaccg caataatggt atctctgtca
     4501 gctatgcatg ttatatgctc catggaactg tgaagagact ctacatattc ttttgcgaag
     4561 tcactgagtt ctcctatggg ggaatatttc ttaagtatta cttcaccttc cctatccgta
     4621 aatatttcca aagggtcccc ttctcttata cgcaaagttc tccttatctc tttaggtatt
     4681 acaactcttc caaggtcatc tatccttctt acaattccgg ttgctttcaa cttaaaacct
     4741 ccttttcgaa tttacataat tgtttgtaat ggttattatt ttaccatcta caatttttta
     4801 tacacaattt tgcaaattat agataataag aaaaaaagca gtcttacgac cgcatttttc
     4861 aagtcatggt gccaggcacg tcagatagac agttatcttc catacttgtc tatcagttct
     4921 ttaatcgcac ttcccgttg
//
LOCUS       DAOOAB010000087         4934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_19430_length_4934_cov_15.887272, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000087 DAOOAB010000000
VERSION     DAOOAB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19430_length_4934_cov_15.887272"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..914
                     /locus_tag="PKU88_12095"
     CDS             195..914
                     /locus_tag="PKU88_12095"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013173749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPX70052.1"
                     /translation="MDSSKLSLLASWMKQSKYTVVLTGAGMSTESGIPDFRSPGGWWR
                     NIDPATVASRYALENQYELFRDFYIMRINTLEGCKPHEGHKVLAEWEKRGLVHCIATQ
                     NVDRFHQEAGSRNVYELHGNIRTVRCEDCSNAAELKEFLEKKPCAKCKGKLRPNVVLF
                     GESLPYEALDNATHSISKADLAVVVGTSLQVYPVNQLPELCKGKKAYINLDTGRSSSQ
                     FDLVIEGSARDVLTKAEMLLD"
     gene            1005..2198
                     /locus_tag="PKU88_12100"
     CDS             1005..2198
                     /locus_tag="PKU88_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPX70053.1"
                     /translation="MRTERLYNDDPYLTEFRAEVIETLTYGDKYGIVLDRTAFYPAGG
                     GQPWDTGYLNDEKVTEVIEKDGKLLHIVDNILLSTEVKGSVDWARRFDFMQQHTGQHI
                     LSACFEKLLNGSTDSFHMGKDIVSIEINIDSFSIDDAERIENMANDIIYSNLPVIARI
                     VSGEELNSLPLRKMPKVTENIRIVEVKETDYSPCGGTHVRTTGEVGMIKIKNWEKCKG
                     GIKFAFVCGNRALKDYGLYNSIIRALCEKLSARDSEIVEAVDKLLFDLRNTEKQLSAS
                     TQELIRIEADNTIKECPVVSGIRLISRVLDNRSINDVKLLAQYLTKVPGTVALLACKN
                     ENAQVIFSRSEDVNMDMNALFKAVLPIIDGKGGGNSRTAQGGGSRVDRLEEFMNNAKN
                     LIISK"
     gene            complement(2242..2583)
                     /locus_tag="PKU88_12105"
     CDS             complement(2242..2583)
                     /locus_tag="PKU88_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70054.1"
                     /translation="MVDNDRDDCFLKEIVVLNEILTPLVSSYRLSVGAAEEFNRIALA
                     HRKDVEDAIDRADDLGHLVDDVRRKLKKCMKIYFSELDYKLEHMEEIREKAAMREKLN
                     SKLNKMSEEKK"
     gene            2744..3667
                     /locus_tag="PKU88_12110"
     CDS             2744..3667
                     /locus_tag="PKU88_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPX70055.1"
                     /translation="MDFNTAVNQILVLFIIMFIGTIAKKSRIIDEGIQNSISVLLMQI
                     ALPSLILSSSNIARTAEVLPNLISILTITSIAYILTILMCMLTVKGLRFDQKTSAVFI
                     SLIVFGNVGFMGYPVVRAFFAETGVFYATASNLVFTAFLWTYGILLFNKQEKIDLKKL
                     LNVGSISTLIAILMFAFRISLPYSLQTAFDLIGKMTTPLSMLLIGVLIAEIDTSKLVS
                     DKRIYLVAAIRLIIIPLATACILRFMGFNTMVISICTLMAAMPSGATNAIFAAQFDTE
                     PLFASVGVFITTLLSILTLPFTVYMLTNFIL"
     gene            3904..4140
                     /locus_tag="PKU88_12115"
     CDS             3904..4140
                     /locus_tag="PKU88_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HPX70056.1"
                     /translation="MSGKRTGRRSSKILVPEAEVAMDTLKKSIASEFGIDFNSDYKGN
                     LSAKQCGKVGGEMVRRIIEQMKQEIAQSPGRFRQ"
     gene            4429..4525
                     /locus_tag="PKU88_12120"
     tRNA            4429..4525
                     /locus_tag="PKU88_12120"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4463..4465,aa:Sec,seq:tca)
     gene            complement(4613..4780)
                     /locus_tag="PKU88_12125"
     CDS             complement(4613..4780)
                     /locus_tag="PKU88_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPX70057.1"
                     /translation="MADKNLTCKDCGTEFVFTEGEQAFYKEKGFENEPQRCPDCRKAK
                     KAQRNDRDFRR"
BASE COUNT         1504 a          962 c         1051 g         1417 t
ORIGIN      
        1 taccaataaa tgcgacagag caaaacagat gtctttaggg cattaaaaag gagcagccat
       61 tcaacgcctg caaggctttg gactttgcag gttttttggt ttactttatt acaaaagcat
      121 atataattaa agtacaggtt aatgtttgag gtaataatcc caaagccgta taagtaatag
      181 aggaggttct tcatatggat agcagtaaat taagcctgct tgcatcatgg atgaagcagt
      241 caaaatacac tgtggttttg accggtgccg gcatgtcaac tgaaagcggc attccggact
      301 tccgttcgcc aggcgggtgg tggagaaata ttgatcctgc aaccgttgct tcaagatatg
      361 cattggaaaa ccaatatgag ctgttcagag acttctatat catgaggata aacacacttg
      421 aaggctgtaa gccccatgaa ggtcataaag tattggctga atgggaaaaa aggggcctgg
      481 tacattgcat tgcaacacaa aatgttgacc ggtttcatca ggaggccgga agccgcaatg
      541 tatatgaatt gcacggaaat atcaggactg tcagatgcga ggactgcagc aatgcagcag
      601 aattaaagga attcttggaa aagaagccgt gtgcaaaatg caaagggaaa ctcaggccaa
      661 atgttgtgct tttcggagaa tcccttccat atgaagcatt ggataatgca acacattcaa
      721 tcagtaaagc tgatcttgcg gttgtcgtag gaacaagtct tcaagtatat ccggtaaacc
      781 agctgccgga gctgtgcaaa gggaagaagg cctatatcaa cttagatacg ggcaggagca
      841 gcagtcaatt tgacttagtg atagaaggaa gcgcaaggga tgttttgaca aaagcagaaa
      901 tgcttttgga ttaatctata aagcatactt tcccatacga taaaaagcaa aaggcattat
      961 tgaactggac ttgcgggaat gctaaacata aaaaggaggc aaatatgaga actgaacgat
     1021 tatataatga cgacccctat cttacggagt tccgggctga agttattgaa acactgactt
     1081 atggagacaa atacggaatt gtactggatc ggaccgcatt ttatccggcc ggaggcgggc
     1141 agccctggga tacgggatat ttgaatgatg aaaaagtaac ggaggtaatt gaaaaggacg
     1201 ggaagctgct ccatattgtt gataatatac tcctgtcaac agaagtaaaa ggctctgtcg
     1261 attgggcaag acgctttgac ttcatgcagc agcataccgg ccagcacata ttgtcggctt
     1321 gctttgaaaa acttttaaac ggaagcaccg acagcttcca tatggggaag gatatagtaa
     1381 gcatagaaat caacatcgac agcttcagca ttgatgatgc cgagcgcatt gagaatatgg
     1441 caaatgatat aatatacagc aacctgccgg taatagcgag aattgtaagc ggtgaagagc
     1501 tgaacagcct gcctttaaga aaaatgccga aggttaccga aaatatcaga atagttgaag
     1561 tcaaagaaac agactactct ccttgcggcg ggacccatgt gaggacaacc ggcgaagtgg
     1621 gaatgataaa gataaaaaac tgggaaaaat gcaaaggagg catcaaattt gcgtttgtat
     1681 gcggaaacag agcgttgaag gactatggat tgtataattc aatcatccgg gctctttgtg
     1741 aaaaactgtc ggccagggat tctgaaattg ttgaagccgt tgacaagctg ctgtttgact
     1801 taaggaatac tgaaaagcag ctttccgctt ccacccagga gttgattaga atcgaagctg
     1861 acaatactat caaggaatgt ccggtagtat caggtatcag gctgataagc agagtcttag
     1921 acaacagaag cataaacgat gtaaagcttc ttgcgcagta tctgacaaag gtaccgggga
     1981 ctgttgccct tctggcttgc aaaaatgaaa atgcacaagt catattcagc cgttccgagg
     2041 acgtgaatat ggatatgaat gcacttttta aagcagtact tcccataatt gacggtaaag
     2101 gcggcggaaa ttcaagaaca gcccaaggcg gaggcagcag agtggatagg ctggaggaat
     2161 ttatgaataa tgcaaaaaat ctgattatct ctaaatagca caataggatg cctgctgcat
     2221 catcaataag cggcaggcac cttatttttt ttcttcagac atcttgttaa gctttgaatt
     2281 aagcttctcg cgcattgcag ccttttcccg tatctcctcc atatgctcca gcttgtaatc
     2341 cagttccgag aaatatattt tcatacactt cttaagcttt ctcctcacat catcaactaa
     2401 atggccaaga tcatccgccc tgtctatggc atcctcaaca tccttgcggt gcgccagggc
     2461 tatcctgtta aactcttcag cagccccgac agacaacctg taggagctta caagaggtgt
     2521 gagaatttca tttaagacca caatttcctt taggaaacaa tcatccctgt cattatcaac
     2581 catattaccc tctcactccc ttgcacccct atttccgtta ttgtacatat atttaaatct
     2641 atgatatatt tatttttata atgcaacttt attacataat atatatagta aaatagatta
     2701 ttatcacaaa agcaggactg tctgctttta tattgaggtt tatatggatt ttaatacggc
     2761 tgtcaatcaa atactggttt tatttattat aatgtttatt ggaacaattg caaaaaaatc
     2821 aagaattatc gatgaaggca tacaaaactc aatttcagtg ctcctgatgc agattgcact
     2881 accttcgcta atactctcat catccaatat tgcaagaact gccgaagtgc tgcctaatct
     2941 gatcagtatt cttacaatca catcgattgc ttatatactg actatactaa tgtgcatgct
     3001 gacagtaaaa gggctgcgct ttgatcagaa aacatccgct gtttttattt cccttatagt
     3061 ttttggaaat gtaggattta tgggatatcc cgttgtcaga gcattttttg cggaaaccgg
     3121 agtcttttat gccacagcct caaacctggt atttactgcc tttctttgga cctatggaat
     3181 actattgttt aacaaacagg aaaaaatcga cctgaaaaag ctccttaatg ttggttcaat
     3241 atcaacttta attgctattt taatgtttgc attccgtata agtctgccat attcccttca
     3301 gacagcattt gatctgattg gaaaaatgac cacacccctc tcaatgcttc taataggagt
     3361 gctaattgcg gaaatagata cttcaaagct tgtttcagac aaaagaatct atctggtggc
     3421 ggcaatcagg cttattataa ttcctctggc aactgcctgt attctaaggt ttatgggctt
     3481 taacaccatg gtgatatcga tatgcacatt aatggctgcc atgccgtcag gtgcaacaaa
     3541 tgcaattttt gccgcacaat ttgacacaga accattattt gcttcagttg gtgtttttat
     3601 aaccacgctg ttaagcattc ttacactgcc attcactgta tacatgctta ctaatttcat
     3661 actttagctt tcattttttc tttctttatc ctatattttc aactcatgca aaagcagaat
     3721 ccatagaata ctataaatgt cgagaaaaat atttataatt gcaaaatcag ataggctatg
     3781 attttgtgtt aaattaatgt acttcccacg acatatgaat aagggaaagt gttttgttca
     3841 aaacatatac tatgaaaggc ggtgtttttg atggcaagaa atactgaaac agcatataag
     3901 actatttcag gaaaaagaac cggcaggcga agctcaaaga ttctcgtacc tgaagccgag
     3961 gtagcaatgg atactttgaa aaagtccatc gcctcagagt ttggaataga ttttaacagc
     4021 gactataaag gtaatctgtc agcaaagcaa tgcggcaagg taggcggtga aatggtaagg
     4081 agaatcatag agcaaatgaa gcaggaaatc gctcaatccc ccggcaggtt tcgccagtaa
     4141 tagaaagggc atatggaagt ttgagacatg ggcgggacac ggggacattt cgggacacgg
     4201 ggacatttcg ggacacgggg acatttcggg acacggggac atttcgggac acggggacgt
     4261 ttctcctgtc ccactgaaag tgggacagga gaaacgtccc cgtgtcccga aacgtcccta
     4321 tgtcccgcgt tcctgtgtcc ttttttttgt gattttcctc ttatattggc attgcttttt
     4381 aaaatgcatt atgataatat ttgtagttgt acaaggcatg cttcatatgg gggtagatgg
     4441 gtgctggtgt gccctctggt cttcaaaacc agtgtttggg gttggtagcc tcatgggtgg
     4501 gttcgattcc cacatactcc cgccaattta aattagcgta ttccttaatt tttgataaat
     4561 aaaaaaagga ctgacactct tagagtgaag tccttttttg ctttgctcca tattatcttc
     4621 tgaagtctct gtcgtttctc tgtgcttttt ttgcttttct gcagtcaggg catctctgag
     4681 gttcgttttc gaagcccttt tccttgtaaa aagcctgctc gccttctgtg aatacaaatt
     4741 ctgttccaca gtccttgcaa gtcaaattct tgtcagccat ttttttacct cctattattt
     4801 attaggattt aacaactttc aaacattata cattctcctg acaaataata ggaaatggtt
     4861 ttaatatcgt taaatcacca gttatataag tataacaccg ataccattta atgtcaacgg
     4921 ttattttcac ttcg
//
LOCUS       DAOOAB010000088         4909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_19540_length_4909_cov_16.603832, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000088 DAOOAB010000000
VERSION     DAOOAB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19540_length_4909_cov_16.603832"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..628
                     /locus_tag="PKU88_12130"
     CDS             14..628
                     /locus_tag="PKU88_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPX70058.1"
                     /translation="MKKNIIIGITAIILVATAVYVTKNYNSKSEIVINPPRQDQQADQ
                     SASGPDSDPAPSSDSEGNSGESKINLMENYDFTLKDLEGNEVKLSSLKGNKIFLNFWA
                     TWCPPCKAEMPDIQKLYQETKDSGLVILAINVDTDKKAAQDFIDANNYNFTVLWDENG
                     EVSRLYQVTAIPTSYFIDTEGFLDAGTTGAISLEAMKEYVNNLE"
     gene            672..1193
                     /locus_tag="PKU88_12135"
     CDS             672..1193
                     /locus_tag="PKU88_12135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX70059.1"
                     /translation="MNNNPTAIIEMENGDVMKLELYPDIAPNTVRNFISLANKGFYDG
                     VIFHRVIPGFMIQGGEPQGVGVGGPGYSIKGEFKANGFENNLKHTKGVISMARSMSPN
                     SAGSQFFIMVADSPHLDGQYAAFGKVTEGIETAEKIAAVKRNRSDKPLEDQRIKKVAA
                     DTKGIEYGEPEKQ"
     gene            complement(1228..2133)
                     /locus_tag="PKU88_12140"
     CDS             complement(1228..2133)
                     /locus_tag="PKU88_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPX70060.1"
                     /translation="MMKTRRRYFGGGAEANDFWPSFTDVMSTLALILFFLMLLAYVQN
                     IIFGNNLENAKQQLEISRAEISSAERELRRIRLEVDKTKTALALSEQEIENQKAIIAL
                     SNEELESLRVRLQGIAFLRLDILQKVKESVEQELGRYNDRGQELVSIGDNANIIINES
                     LVFAYNSYKLKPEAEDLLTQLAKAFERVLENPEVRENIDAISIDGHADSMGGSAYNRE
                     LSARRATEVINYMFKVNPNLEDKYGEYFAANGFSEFRPISTGNDEESRAANRRIEISI
                     IVKDSNIQKVIEKYLNETEEIFEER"
     gene            complement(2130..3182)
                     /locus_tag="PKU88_12145"
     CDS             complement(2130..3182)
                     /locus_tag="PKU88_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70061.1"
                     /translation="MEILTKSISNLGYIAYSIICLIAIIFVVSFITMVFVRGKYKGLQ
                     NELSSAAERRDARFRSHVLSDIVEDYKTISLASKGEINTQAIVEKNLSIKLRGLGLGE
                     RFVKNSVSLMVVLGLLGTFYGLTMSVGRLVELLGTSGNSDILVSMDSVVTGLISSVRG
                     MAVAFSTSLAGVGGSIVITVLGIVINIEESRQSLMVQLEDYLDNTVEQELLPKKESEL
                     SRISRVIESSFDGFSSRVEDILRSTVLDFSDKLAAASSNIENSARCLDETIIKFEKAL
                     SVFSSNTRDFSEFNHNLRGNIERMDVSFINLRESIAETSKNIASNQEAMNSFSAAVQQ
                     AAAVIGSEKGTGSGGR"
     gene            3522..4880
                     /locus_tag="PKU88_12150"
     CDS             3522..4880
                     /locus_tag="PKU88_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPX70062.1"
                     /translation="MDKIMELNGIINGFVWGPYMLIFLVGTGVYLTFRTNFMSITKLG
                     YILKSTLFKMFKKGGEGEGEITAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WVSAIFGMTTKFGEVVLSINYREKTPDGRFVGGPMYYIEKGLKMKWLAYVFAAFGAIA
                     AFGIGNMVQSNSVAASLEVSFGVNKMVTGIVLAVLSAVVIIGGIKRIAQVTERLVPFM
                     AAFYILGGLFIIISNISHVGEAFGLIFGSAFTGTAALGGFAGSTMQMAITKGIARGVF
                     SNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFVDTIIICSITALSIILTGVWSSGET
                     GAALTTAAFEATIPGGGYIVSISVLLFAFSTILGWAYYGERCAEYLFGPKINMAYRIL
                     WIPFVAIGAIGGLNFLWDLADTLNGLMAIPNLIGVMGCSGIIIKLTKEFFAKQYVEEE
                     KK"
BASE COUNT         1404 a         1104 c          981 g         1420 t
ORIGIN      
        1 aggagaattg tttatgaaaa aaaatattat tattgggatt acagctatta ttcttgtagc
       61 tacggctgtt tatgtaacta aaaattacaa ttccaaatcc gaaattgtaa taaaccctcc
      121 ccggcaggat caacaggcag atcaatcagc ctccggccct gactctgacc ctgcaccttc
      181 ttccgattcg gagggcaata gcggtgaatc aaagataaat ttgatggaaa actatgattt
      241 tacccttaag gacttagagg gcaatgaagt aaaactcagc agccttaagg gcaataaaat
      301 attcctcaat ttctgggcta cctggtgccc gccctgcaag gcggaaatgc cggatataca
      361 gaagctttac caggagacaa aggacagcgg ccttgtaata ttggcaataa atgtcgatac
      421 agataaaaaa gccgcacagg actttattga tgccaacaac tataacttca ccgtcctgtg
      481 ggatgaaaac ggagaggttt cccggctata tcaggtcaca gcaataccta cttcatattt
      541 tatcgatacc gaaggcttcc ttgacgccgg aaccacaggt gcgatatccc tggaagcaat
      601 gaaggaatat gtcaataatc tggaataagc ctttaaatac ttcagaacaa acagaaagag
      661 gatgatagta tatgaataac aaccccacag caattataga gatggaaaac ggagatgtga
      721 tgaagctgga actgtatccc gatatcgccc ccaatacggt aaggaatttt atatcccttg
      781 caaataaagg cttttatgac ggagtcatat tccacagggt tatccccggt tttatgatac
      841 aggggggaga gccccagggt gtgggtgtcg gcggtcccgg gtacagcatc aagggcgaat
      901 ttaaagcaaa cggctttgaa aataacctga aacatactaa gggtgttata tcaatggcaa
      961 gatcaatgtc ccccaattcg gcaggctcac agtttttcat aatggtcgca gattcacctc
     1021 acctggatgg ccagtatgcc gcttttggga aagtaacaga aggtattgag acagcggaaa
     1081 aaattgcggc agtgaaaaga aaccgctctg acaaacccct tgaggatcag agaataaaga
     1141 aggttgcggc agatactaaa ggtatagaat acggtgaacc ggaaaagcaa tgaggctgat
     1201 gacaaagtca tcagcctcat tcttccgcta tctttcttca aatatttcct ctgtttcatt
     1261 cagatacttc tcaataacct tttgtatatt tgaatccttc actattatgg aaatttcaat
     1321 acgcctgttg gcagccctgc tttcctcgtc atttcccgta cttatggggc ggaattcaga
     1381 aaaaccgttg gccgcaaaat attctccgta cttgtcctcc aaattgggat tcactttgaa
     1441 catgtagttt attacctctg tagccctcct ggcggataat tccctgttat aagccgaacc
     1501 tcccatgctg tcggcatgtc cgtcaatgct tatggcatct atgttttctc ttacctcggg
     1561 gttttccagc actctttcaa aggccttggc caattgtgtc aggaggtctt ccgcctccgg
     1621 cttaagcttg tatgaattat aagcgaaaac aaggctttca ttaattataa tatttgcatt
     1681 gtctcctata gataccaact cctggcccct atcattatac ctccccaact cctgctctac
     1741 agattcttta accttctgca gtatatccag cctcagaaag gctatgccct gaaggcgtac
     1801 tctgaggctt tccagctctt cattgctcaa agcgatgatt gctttctggt tctcaatttc
     1861 ctgctcagac aatgccagag ccgtctttgt cttatctacc tccagcctta ttcgtctcag
     1921 ctctctttct gcgctgctta tttccgcccg tgatatctcc agctgctgct ttgcattttc
     1981 aagattgttc ccgaatatta tgttctgtac ataggccagc agcataagaa agaacagtat
     2041 caatgccaag gtagacatta catctgtgaa ggacggccag aaatcattgg cctcggctcc
     2101 tcccccaaaa tatcttctcc ttgttttcat catctgcccc cacttcctgt gcctttttca
     2161 ctgccaataa ctgctgccgc ctgctgcaca gccgccgaga aactgttcat tgcttcctga
     2221 tttgaagcaa tattctttga agtctccgca attgattccc ttagatttat gaaggataca
     2281 tccattcttt caatattacc cctcaggtta tgattgaatt cactgaaatc tctggtattg
     2341 ctgctgaata ccgaaagcgc tttttcaaac tttatgattg tctcatcaag gcatcttgcg
     2401 gaattctcga tattgctgct ggctgccgca agcttgtccg aaaaatccag tactgtgctg
     2461 cgcagaatat cctccaccct tgaactgaaa ccgtcaaaag aggactcgat gaccctgctg
     2521 atgcggctaa gctccgattc cttcttcgga agaagctcct gctccacagt attatccaga
     2581 tagtcctcca attgaaccat gaggctttgt cttgactcct cgatatttat cacaattccc
     2641 aaaaccgtaa tcactataga acccccaaca cccgcaagac ttgttgagaa agctaccgcc
     2701 atccccctta ctgaagaaat aagccctgtt acaacagaat ccatactgac aagtatatcg
     2761 ctgtttccgc tggtacccag cagttccacc agtctcccga ctgacattgt aagcccgtag
     2821 aaggttccca ggagccccag caccaccatc aatgaaactg agttctttac aaaccgctct
     2881 cccaatccca gccctctaag ctttatgctt aaatttttct ctactattgc ctgagtgttg
     2941 atttctcctt tggaagccag agatatggtt ttataatcct ctactatatc gcttaataca
     3001 tgactcctga acctggcatc ccttctttct gcagccgacg acaattcatt ctgcagcccc
     3061 ttgtatttcc ccctgacaaa caccattgta ataaaggaaa ccacaaatat tattgctatt
     3121 aagcatatga ttgaatatgc tatatagccc aagttactta tactctttgt taaaatttcc
     3181 aagaatcacc ccacctttca attttttgag tgccaggatt cttttatcag cttctattta
     3241 ctattttata gaaaacgatg aaatttatca ataaattaaa aatttaaaaa attactgcat
     3301 aatttcttgc ttgcattgta ttattataga gataacaaga aattaactga aaattccaac
     3361 aattcaatat aatgaatatt gtataaaaat atatattgta aaatactact attattgata
     3421 aaataaatta tacaatatta ttattctgag cattatatat ctcccctaaa tttgttattt
     3481 gggaagatat ttaatgtata aaaataacaa ggaggtattc catggacaag ataatggaac
     3541 tcaacggtat tattaacggt tttgtatggg gcccttacat gttgatcttc ttagttggta
     3601 caggcgttta tctcactttt cgcactaatt tcatgtcaat tacaaaacta ggttacatac
     3661 ttaagagcac tctctttaaa atgttcaaaa agggaggaga aggagaaggc gaaatcaccg
     3721 cattccaggc tgttgcaaca gcgcttgcag caacagtcgg tacaggtaat atcgccggag
     3781 ttgctacagc tatagcccta ggaggtcccg gagcagtatt ctggatgtgg gtatctgcta
     3841 tatttggaat gaccactaaa ttcggagaag ttgtactttc catcaattac agggaaaaga
     3901 caccggacgg gcgtttcgtc ggcggtccta tgtattacat tgaaaaggga cttaagatga
     3961 aatggctggc atatgtattt gcagcatttg gtgcaattgc agcctttggt ataggcaaca
     4021 tggtccagtc aaactccgtt gctgcttccc tggaagtatc ctttggagtt aataaaatgg
     4081 ttaccggtat tgttttggca gttttgtcag cagtagttat aatcggcggt atcaagagaa
     4141 tagctcaggt tacagagaga ttggttccgt ttatggcagc attctatatt cttggcggct
     4201 tgttcataat aatctctaat atttctcatg taggagaggc tttcggatta atattcggaa
     4261 gcgcatttac aggaactgca gccctcggcg gctttgcagg ctcaacaatg cagatggcca
     4321 taacaaaagg tattgcccgc ggagtattct ccaatgaggc aggtcttggc agcgcaccta
     4381 tagctcatgc tgcagctaca accgaccatc ctgtacgtca ggggctttgg ggagtatttg
     4441 aagtatttgt agatacaata ataatctgtt caattaccgc attgtcaatc atattaacag
     4501 gtgtatggag cagcggcgaa acaggtgcgg cattgaccac agcagcattt gaagctacta
     4561 tacccggcgg cggatatata gtctccataa gtgtactgct gtttgcattc tccacaattc
     4621 tcggctgggc atattacgga gagcgctgcg cggaatatct gttcggaccc aagatcaata
     4681 tggcatacag aatcctgtgg ataccgtttg ttgctatcgg tgccatcggc ggcttgaact
     4741 tcctctggga tttggcagat acactgaacg gcttgatggc tatcccgaac cttataggtg
     4801 tgatgggttg cagtggaatt attatcaagc ttaccaagga attctttgca aaacagtatg
     4861 tagaagaaga aaagaaataa aacataataa agtcaaaaaa cgagagttt
//
LOCUS       DAOOAB010000089         4855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_19810_length_4855_cov_15.075625, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000089 DAOOAB010000000
VERSION     DAOOAB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19810_length_4855_cov_15.075625"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..1328)
                     /locus_tag="PKU88_12155"
     CDS             complement(477..1328)
                     /locus_tag="PKU88_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX70063.1"
                     /translation="MMDAAVIGGGPAGLSAALNLKIRNKSLVFLGGSENVTGLMKAPQ
                     IDNYPGLKGISGAELVKSFRDHAISMGVEIKKEKAYNILDMGDYFSINAGNEFYEARS
                     VIIATGREKAKYLEGEKEYLGKGIGYCATCDGPLYRNKSIAIISENEEGEEEANFLSE
                     ICSEVFYIPSYNTEPKLKDSIKLLKEKAIGIQGDGNKVNKLTTDKRELKVDAVFIVRD
                     VIPVNQLIEGLELHEGSIKVDRDMAASIKGVFACGDCTGKPYQIAKAVGEGNVAALSA
                     VRYLEKK"
     gene            complement(1346..2311)
                     /locus_tag="PKU88_12160"
     CDS             complement(1346..2311)
                     /locus_tag="PKU88_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPX70064.1"
                     /translation="MELQDSGRLSIFNGNLLYMIVMILFVTVGYIAQQRDFHYGILIT
                     EFLLIALPAIIYVKINGASVKKELRFNSLSLTDAILVIVAAVAAYFAAVFINLIAEIL
                     ISLMGDLIVPDIPFAKNPGEYLVLLFVIAGSAGICEEILFRGFILRSYEKLGMWPDIV
                     FTALLFSVLHLNIQNMAAPFFLGIILGFVVYKTNSIYAGMLGHFINNAVSVTWGYVIM
                     NLPFYESMDIEQLQGGMTTQSLIGASIIFGLILPIAGTILAICLKAIKDRHPEAASPR
                     PDESFVSTMGNIRLSWPLLVSLLIFIGMMIMEILLITNGRRPINF"
     gene            complement(2337..3245)
                     /locus_tag="PKU88_12165"
     CDS             complement(2337..3245)
                     /locus_tag="PKU88_12165"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPX70065.1"
                     /translation="MKRLCLLLAFIMLFTSGCFLMPGTERESVKLYYAVKGNAGLDIE
                     NREIEYKDAKSKYANTLEELLKGPSDSGKFETSISRDTKVLSVKVEGDNLTVDFSKEF
                     NVFSGSMHEAAVVSSVVDTMLQFNELKKVRILVEGQELIAPSGEPYGFMEFIDFNIGD
                     MAEKEITLYFADSQAMYVVPEKRTVFVKKDISDAEFFRIVLEELIKGPNTENLYGTIP
                     EGVTVEYTEVEGDLLKVDFSEEMHTNHWGGAAGESMTINSIVNTLTEFENIKRIMPTV
                     DGGPMSIEHMVVEEPLTRNESMIYKP"
     gene            complement(3266..3955)
                     /locus_tag="PKU88_12170"
     CDS             complement(3266..3955)
                     /locus_tag="PKU88_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HPX70066.1"
                     /translation="MKTARVVFAVLFIILVPPVLLLTDVQMVAYDRDYYRAEYDKYSI
                     PGHIGMSMDNLMEATEKLLLYLDNKRDDLNFKASFNHGEEEFFSPRDKLHMIDVRGLF
                     VRGRLFRDISLIYIAVFIMLLFRGKPFRNQLRKLARHGIAISAAGIAPVIVLIILMKI
                     DFYKYFTVFHEIFFTNDLWLLDPAADRLINIFPQDFFTDMAFSISYLYIAEMAAILIC
                     SLIALKLVKSD"
     gene            complement(3961..4683)
                     /locus_tag="PKU88_12175"
     CDS             complement(3961..4683)
                     /locus_tag="PKU88_12175"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPX70067.1"
                     /translation="MKTIGIIGGMGPLAAARLFERIVILTKAGCDNEHIPIIIDNNTN
                     IPDRTEAILNNGDNPAYELVRSALRLELMGADAIVMACNTAHYFYEEIIKFVKVPVIN
                     MVEETAKYIIKSNPAVKCAGLLATEGTCKSGIYRKVFEKHGLNLLVPEAEEQQHITEL
                     IYDIKRSREDINPENTIRVVSALRERGAEVIILGCTELPIAFGMFDICSGYVDSLEVL
                     AMSAIAFAGKDIRYEKINKGLK"
BASE COUNT         1305 a         1226 c          861 g         1463 t
ORIGIN      
        1 ccacaactat tttcaagcct ttctgttcat atctgtgaag cataagtgac ccccggatta
       61 ataaaattac atcgcagaat tattggttct attgtgtgtc actgtttatt ctataataat
      121 ttgcggggtg tgtcaatcaa gccgcggcag aagataagcc gcccaaagga cggcaatgct
      181 actgcaaatt cttattcaag tcgtccacca gttcttccgg atctatccga tatctgagag
      241 cagcctgctc tactgtcata tacagagcac cgcctcatcc gaagcacttt attccgtgtt
      301 ccttgaatac tgtctcaagc tgcggatatt tataaaggat atcgtctata accatattct
      361 tactaagcat tttttcaccc cttaatatta tatgtaatta tcaatatatt atatacctat
      421 acggggtata tgttatctta gtattattat attattactt ccttatgctg tcaatattat
      481 tttttttcga gatatcgcac cgcactcagg gctgccacat ttccttctcc cacggctttc
      541 gcaatctgat aaggctttcc ggtacaatcc ccgcaggcaa aaacaccctt aatgcttgca
      601 gccatatccc tatccacctt tatagagccc tcatgaagct ccaatccttc tataagctgg
      661 tttacaggta ttacatccct gacgatgaat accgcatcca ctttaagttc ccttttatcg
      721 gtagtcagtt tgttgacttt atttccgtcc ccttgaattc ctatggcctt ttccttaagc
      781 agcttaatac tgtccttaag cttcggctcc gtattataag aaggtatata gaatacctcg
      841 gagcaaatct cggaaagaaa attagcttct tcttcccctt cttcattctc tgaaattata
      901 gctatcgact tgttcctgta taacgggccg tcgcaggtgg cgcaataacc tatccctttt
      961 cccaggtatt ccttctctcc ctcaaggtac tttgcttttt cacgtcctgt ggcaataatg
     1021 actgaccgcg cctcatagaa ttcatttccg gcatttattg agaaataatc tcccatgtca
     1081 agtatgttat atgctttttc cttttttatt tccactccca ttgaaattgc atggtcccgg
     1141 aagctcttaa caagctccgc tccgcttatg cctttcaggc cgggatagtt gtctatctga
     1201 ggcgctttca taaggcctgt tacattttca ctgcctccga gaaatacaag gctcttgttt
     1261 cttattttta ggttcaatgc ggcggaaagg ccggcaggcc cgcctccgat aacagctgca
     1321 tccatcattt ttcatcattc cttttttaga aatttattgg tcttcttcca tttgtaataa
     1381 gcagaatttc cataatcatc attccgatga atattaaaag agatactaat aaaggccagg
     1441 acagccttat gtttcccata gtacttacga agctttcatc aggcctcggg gaggccgctt
     1501 ccgggtgcct gtcctttatc gccttcaggc aaattgccag aattgtaccc gcaatgggca
     1561 atatgagccc gaaaataatt gaggcgccta ttagggattg tgtggtcatg cctccctgaa
     1621 gctgttcaat atccatgctc tcataaaagg gcaggttcat aattacatag ccccaggtaa
     1681 ccgaaaccgc attgttgatg aaatgcccga gcattcccgc ataaatggaa ttcgtcttgt
     1741 ataccacaaa accaagtatt atacccagaa aaaacggtgc cgccatgttc tgaatattaa
     1801 gatgcaatac agaaaaaagc agtgccgtaa aaactatatc cggccacatt cccagctttt
     1861 cataggacct caatatgaag cccctgaaca atatttcttc gcaaatgccc gcagaacctg
     1921 caataacaaa aagaagcact aaatactctc ccgggttttt cgcaaagggt atatcaggta
     1981 caataagatc ccccatcagg cttataagga tttctgcaat caggttgatg aaaacagccg
     2041 caaaatatgc ggcaacggca gcaacaatta caagaattgc gtcagtgagg ctcaagctgt
     2101 tgaacctcag ctcttttttc actgaggcac cgtttatttt cacatatatg attgcaggca
     2161 gggcaatcaa aagaaattcg gttataagaa ttccataatg aaaatccctc tgctgtgcaa
     2221 tataccctac tgtcacaaac agaatcatta ctatcatata taaaagattg ccgttgaaaa
     2281 tagacaacct gcctgaatct tgcagctcca atattaatcc tcctgtaatc ccgtgcctat
     2341 ggcttatata tcatactttc attccttgtc agaggttctt ccaccaccat gtgctctatg
     2401 ctcatagggc ctccgtccac tgtaggcatt atccttttga tattctcaaa ttccgtcaat
     2461 gtgttgacta tggagttaat ggtcatactt tctcctgctg ctccccccca gtgattggta
     2521 tgcatttcct ccgaaaagtc aaccttcaga agatctcctt ccacctcggt atattcaact
     2581 gtgacccctt ccggtattgt cccgtacagg ttttcggtat tgggcccctt aatgagttcc
     2641 tcaagcacta tccggaaaaa ttcagcatcg cttatgtcct ttttcacaaa aacagttctt
     2701 ttctccggaa ccacatacat agcctgggag tcggcaaaat atagagtaat ctctttttca
     2761 gccatatctc cgatgttgaa atcgatgaac tccatgaatc cgtatggctc tccgctggga
     2821 gcaatcagtt cctgtccttc aacaagtatt ctcactttct taagctcatt aaactgcagc
     2881 atagtatcaa ctacggaaga taccactgcc gcttcgtgca tgctccccga aaaaacattg
     2941 aactcctttg aaaagtcaac tgtcaggtta tctccttcaa ccttgacact cagcactttt
     3001 gtatccctgc tgatgcttgt ttcgaatttg ccgctgtcgg aagggccttt caacagctcc
     3061 tcaagggtat ttgcatattt gcttttcgca tccttatatt caatttctct gttttctata
     3121 tccaagccgg cattgccctt tacggcataa tacagcttga cgctttccct ttctgttccc
     3181 ggcatcagaa agcagcctga ggtaaacagc ataataaaag caagcaataa acacaacctt
     3241 ttcataataa aacctccatg atagtttaat cggacttgac aagcttcagg gctatcaagc
     3301 tgcaaatcag tatggcagcc atttctgcaa tgtacaggta tgatatgctg aaagccatat
     3361 cagtaaagaa atcctgggga aaaatattta tcagcctgtc tgcggccgga tccaacagcc
     3421 acaggtcatt tgtaaaaaat atctcgtgaa ataccgtaaa atacttgtaa aaatcaattt
     3481 tcatcagtat tataagcact ataaccggag caatacctgc cgccgaaatt gcaatgccat
     3541 gacgggcaag cttccttagt tggttcctga aaggctttcc cctgaacaag agcataatga
     3601 ataccgcaat ataaatcagc gaaatatctc tgaataagcg ccccctgaca aaaagtcccc
     3661 tgacatcaat catatgcagc ttatccctgg ggctgaagaa ttcttcctcg ccatggttaa
     3721 atgaggcttt gaaattcaaa tcatcccttt tgttgtcaag gtataaaaga agcttctccg
     3781 ttgcctccat aagattatcc atgctcatgc caatatgccc ggggatgctg tatttatcgt
     3841 attcagccct gtagtaatcc ctgtcataag ccaccatctg cacatccgta agcagcaata
     3901 ccggcggcac caatatgata aacaggaccg caaaaaccac tcttgctgtt ttcatatgca
     3961 tcacttcaaa cccttattta ttttctcata tctgatgtcc ttccctgcaa aggctattgc
     4021 gctcattgcc agaacctcca gagagtctac atagccgctg catatgtcaa acatgccaaa
     4081 ggctatggga agctctgtac aaccaaggat tatcacttcg gcacctcttt ccctcagagc
     4141 agacactact ctgattgtat tctccggatt tatatcctcc ctgcttcttt ttatgtcata
     4201 gatcaattct gttatgtgct gctgttcttc tgcttcaggc accagcagat tcaatccgtg
     4261 tttttcaaat accttccggt atatccccga cttgcaggtg ccttctgtgg caagcaaacc
     4321 cgcacacttt acggccggat tgcttttaat aatatatttg gcagtctcct ccaccatgtt
     4381 tataacagga accttgacaa acttgataat ctcttcataa aaatagtgcg cggtattaca
     4441 ggccattact atagcatccg cccccataag ttcaagcctc agtgcggatc tgaccagctc
     4501 ataggccgga ttatccccat tgttcagtat ggcttctgtc ctgtcgggta tatttgtatt
     4561 attgtcaatt attattggaa tatgttcatt atcacagccg gcttttgtaa gaattacaat
     4621 ccgttcaaac agcctggcgg cagccagcgg ccccattccg cctattatcc cgatagtctt
     4681 catatgtttt ctcctctgtt attcgatcat tacaattatt ataacaaata gaatcctcat
     4741 ataacaagag gacacgggga cgtttctcct gggacacggg gacgtttctc ctgtcccgcc
     4801 ttcagcggga caggagaaac gtccccgtgt cccaggagaa acgtccccgt gtccc
//
LOCUS       DAOOAB010000090         4812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_20025_length_4812_cov_15.718100, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000090 DAOOAB010000000
VERSION     DAOOAB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20025_length_4812_cov_15.718100"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /gene="lysS"
                     /locus_tag="PKU88_12180"
     CDS             complement(<1..1484)
                     /gene="lysS"
                     /locus_tag="PKU88_12180"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPX70068.1"
                     /translation="MANDEMNLNEILKIRREKFEQLKAMGRNPFEILKYDQTHHSKDI
                     KGNFEEMEGKDVSIAGRIMSFRDMGKASFCDIQDRYGRIQAYIKQDELGEEEYKIFKT
                     YDIGDIVGIKGFVFRTKRGEISVHAKQVTLLCKSLQPLPEKWHGLKDTEIRYRQRYVD
                     LIVNEDVRETFYKRTKIIKAIREFLDARDFIEVDTPILATIASGAAARPFITHSNALD
                     IDLYLRIATELYLKRLIVGGFERVYEMGKDFRNEGIDVRHNPEFTMIELYQAYADYND
                     MMEITENLIAYCAEKVLGTTKINYQGTELDLTPPWNRMTMVEAVKKYSGVDFNEIKSD
                     EEARETAKKLKVLDELKKKLKDCTKGDILNAVFEAYVEEHLIQPTFILDYPVEISPLT
                     KKKNDDPTMTERFEAFIFAREIANAYSELNDPIDQKERFEQQANDKELGDEEAYATDD
                     DFVNSLEIGMPPTGGLGIGVDRIIMFLTDSYSIRDIILFPTMKP"
     gene            complement(1496..1969)
                     /gene="greA"
                     /locus_tag="PKU88_12185"
     CDS             complement(1496..1969)
                     /gene="greA"
                     /locus_tag="PKU88_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPX70069.1"
                     /translation="MGKQNILTIEGLAKLEDELDFLKSKKRAEIAQRIKQALAFGDIT
                     ENSEYDEAKNEQAFVEGRIVQIENILKTAKVIDDEDIQTDIVSVGCRVTLRDVELGDE
                     VEYLIGGSAEADPINLKISNESPVGKALIGKIIGNIVDIVVPDGVIKYEILNIKK"
     gene            complement(2292..3134)
                     /locus_tag="PKU88_12190"
     CDS             complement(2292..3134)
                     /locus_tag="PKU88_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70070.1"
                     /translation="MNNAAIIVFRKSTLPKILKFFAIPLVNRIIDSNNEKDSVRVYII
                     ECPVMKYPLDNRRVEKSLCSLCIENNISFFIGRNTVNYSGSGLEHMENRIERGDLDEI
                     KGMKGLAALIKLSVIRNSNLLNRNMCFIGKSYNYGYISTMLEEATGVFIYEHDQVTNS
                     IKKTIFEKLMLERGISAVFTKDLGRAISQCDIIAADDSVDLESYKPMLKGKILIGENT
                     VSGDFEKIERVLLWYESLEGVTEDNSIIYYNDELLGILRHFYKERNIIDFIRRFPYIL
                     MSRS"
     gene            complement(3186..4127)
                     /locus_tag="PKU88_12195"
     CDS             complement(3186..4127)
                     /locus_tag="PKU88_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HPX70071.1"
                     /translation="MKHIVSISIGSSRRNHKVSMNINNEEIIVERIGTDGDIQMAIKL
                     IRELDGKVDAFGMGGIDVYLSAGKARYRIKDAVPILEAAKITPMVDGSGLKMSLEKDV
                     PGFINDNVEDITGKSVFIMPAIDRYGMAEGFQNLGCELVLGDLMVALGINIPIRSLRM
                     LDKIASIIAPIACRLPFEMLYPTGKEQNKGTSRAGKIDRFFYEADIIAGDFHYIRQYM
                     PGGMQGKMVVTNTVTDEDIEWLKKCGIRTLVTSTPNLGGRSFGTNVIEAVLVALIGKS
                     IDEITGEDYLGMLKEIDFKPRVEQFEEGELFKEEYIR"
     gene            complement(4135..4659)
                     /locus_tag="PKU88_12200"
     CDS             complement(4135..4659)
                     /locus_tag="PKU88_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX70072.1"
                     /translation="MDLVRIGEKIISIPRINNKINEILQYRMLGMSQQEAADKLGVER
                     TFISRLEGMGEIRRGQSIAAIGFPIKNRTEVEKVLRAFGVEYFFLMSEAERTDYVNNC
                     TGAQLTNRIIELVNKLRNFDIVIVMVSDYRNRLARNLLDNKVIEIDIGKSPIKEDVNV
                     NLDNLTDILKTLKG"
BASE COUNT         1235 a         1187 c          751 g         1639 t
ORIGIN      
        1 ataggcttca ttgtcgggaa cagtattata tccctgatag aataggaatc tgtaaggaac
       61 attattattc tgtccacccc tatcccaagt cctcctgtag gaggcatccc tatttcaagg
      121 gagttaacaa agtcatcatc cgttgcatat gcctcttcgt ctccaagctc tttgtcattt
      181 gcctgctgct cgaacctttc cttctggtca attgggtcgt taagctcgga ataagcattg
      241 gcaatttctc tggcaaatat gaacgcttca aacctctctg tcattgtagg atcgtcattc
      301 ttcttctttg taagaggtga tatttcaacc ggataatcca atataaaagt cggctgaata
      361 agatgctctt caacgtatgc ttcaaaaacc gcattcaata tatcgccttt ggtgcagtcc
      421 ttaagcttct ttttaagttc atccagcacc ttaagcttct ttgctgtttc tcttgcttct
      481 tcatcagatt ttatttcatt aaagtcaaca cccgaatact tcttgacagc ttccaccatg
      541 gtcattctgt tccagggggg tgtcaaatcc aattcggttc cctgatagtt gatttttgtt
      601 gttcccagaa ccttttccgc acaatatgct ataagattct cggtaatctc catcatatcg
      661 ttgtagtccg cataggcctg gtatagctca atcatcgtaa actccggatt gtgccttacg
      721 tctataccct catttctgaa gtctttgccc atttcatata ctctctcaaa gccgccgact
      781 atcagcctct ttaaataaag ctcggtggca attctgagat ataaatctat atccaacgca
      841 ttgctgtggg tgataaaagg ccttgcggca gctccgctcg caattgtagc cagtattgga
      901 gtatcaactt caataaagtc ccttgcatcc aggaattccc gtattgcctt tattatcttg
      961 gtcctcttgt agaatgtttc tcttacatcc tcatttacaa tcagatccac atatctctgc
     1021 ctatatctga tttcagtatc cttaagcccg tgccatttct ccggcagcgg ctgcagcgac
     1081 ttgcacagca aggttacctg ctttgcatgt actgatatct cgcctctttt ggtcctgaac
     1141 acaaaaccct ttataccgac aatatcacct atgtcatagg tcttaaaaat cttatattcc
     1201 tcttcaccca gttcatcctg ctttatgtat gcctgaattc ttccgtatct gtcctgtata
     1261 tcacaaaatg atgcctttcc catatccctg aaggacatta tccttccggc tatggaaaca
     1321 tcctttcctt ccatttcttc gaaattgcct ttgatatctt tagagtgatg ggtctgatca
     1381 tacttcaata tctcaaaggg attgcgtccc attgccttca gctgttcaaa cttttccctt
     1441 ctaatcttca gtatctcatt gagattcatt tcgtcattag ccatgcttac tccccctatt
     1501 tttttatgtt tagtatttca tatttgataa ctccgtcagg tacaacgata tcaacaatat
     1561 tgccgataat cttgcctatc aaggccttgc ccacgggaga ttcgtttgag atcttgagat
     1621 taatgggatc cgcctcagct gagcccccaa tcaagtattc aacctcatcg cccagctcaa
     1681 catctctcag ggttaccctg cagcctacac ttacaatatc cgtctggatg tcctcatcat
     1741 ctattacttt ggcagtctta aggatgtttt ctatctgtac tattcttcct tctacaaatg
     1801 cctgttcatt tttagcttca tcatattccg aattttcggt tatgtctcca aaagccagtg
     1861 cctgctttat tctctgcgca atctcggccc tttttttgct ctttaaaaaa tccaattcat
     1921 cctcaagctt agccagacct tctattgtta gtatattttg tttacccaat gtctcctgcc
     1981 tcccgttcca taaaaattac ttatacagat aaaatctggc ttgttcccca gccagtacaa
     2041 tgaaacccta taatattttt atttatgata atatgtactt aaataatttt tttatgctgt
     2101 gcaggtattt ccataattat aaattttcaa tgaagacaca gcattctttt tctttatttg
     2161 tttattataa tgtagtgctt tccttatgtc aagcattagt gaaagcgcta aaataagcaa
     2221 ggaggtgcat gctccggctg atatgcctgt taatactttg aaagattagt aatttgttat
     2281 gttaataagc actagctgcg agacattaaa atgtaaggaa acctccttat aaaatctata
     2341 atattcctct ctttgtaaaa atgcctcaat attccaagca gttcatcgtt ataataaata
     2401 atgctgttgt cttcagtcac tccttccagg ctttcatacc ataacagcac tctttctatt
     2461 ttttcaaaat cccctgatac agtattttcg ccaattaata ttttaccttt tagcatcggt
     2521 ttatatgatt ccagatctac actgtcatct gccgcaataa tatcacactg agaaatcgct
     2581 ctgcccagat ctttggtaaa caccgcagat attcccctct ccagcatcag cttttcaaaa
     2641 attgttttct taatgctatt ggtcacttgg tcatgctcat aaataaaaac ccctgtcgct
     2701 tcctccagca ttgtacttat atatccatag ttgtatgact tcccgataaa gcacatgttt
     2761 ctattcagaa ggttagagtt tctaataaca gaaagcttaa taagggcagc caggcctttc
     2821 atccccttaa tttcgtcaag atctcccctt tctattctgt tctccatatg ttcaaggccg
     2881 cttcccgaat aattcactgt attccttcct atgaagaaac tgatgttatt ctcaatgcac
     2941 aagctgcaaa gggatttttc caccctcctg ttatctaaag ggtacttcat tacagggcat
     3001 tcaataatgt acaccctgac gctgtccttt tcattatttg aatcaataat ccggtttacc
     3061 aggggtatcg caaaaaactt caatattttc ggaagagtgc ttttcctaaa aacaattatt
     3121 gccgcattat tcattgcatc ccctccgaat atattgaaaa ttgctaatat aataatatta
     3181 actgcctatc ggatatattc ttccttaaac aattctcctt cttcaaattg ttccactctt
     3241 ggtttgaagt ctatctcctt cagcatgccc aagtaatcct caccggttat ttcatcaatg
     3301 cttttgccta tcaaagccac cagcaccgcc tcaatgacat tggtgccaaa ggagcggcct
     3361 cccagattcg gcgttgatgt aaccaaggta cgtatcccgc attttttaag ccactctata
     3421 tcttcatcgg tgacggtatt tgtaacaacc attttcccct gcatcccgcc gggcatatat
     3481 tgccttatat aatgaaaatc ccctgcaata atatcagctt cataaaagaa tctgtctatt
     3541 ttgcccgccc tgctggttcc tttattctgc tccttcccgg tagggtacag catttcaaag
     3601 ggaagccgac aggcaatcgg tgcaatgata cttgctattt tgtccagcat cctcagagat
     3661 cttatcggta tatttatacc caaggcaacc ataagatctc caagcacaag ctcgcagccc
     3721 aggttttgaa agccctcggc catgccgtac ctgtcaatgg caggcatgat aaatacactc
     3781 ttacccgtga tatcttccac attgtcgtta ataaagcccg gtacatcctt ctcaagagac
     3841 attttaaggc cggagccgtc aaccattggt gtaatcttcg cggcttccag tattggaact
     3901 gcgtccttta tcctataacg ggccttcccg gcgctaaggt aaacatcgat tcctcccatt
     3961 ccaaaggcat cgacctttcc gtccaattcg cgtataagct tgattgccat ctgaatgtcc
     4021 ccgtcagtgc ctatcctttc aacaataatc tcttcattgt tgatattcat ggacactttg
     4081 tggtttcttc tggatgagcc tatacttata ctcactatat gcttcatacc atatctatcc
     4141 tttcaatgtt tttaatatat ctgttaaatt atccaggttc acattgacat cttcctttat
     4201 aggcgatttt cctatatcta tctctataac cttgttatcc agcaagtttc tggccaatct
     4261 gttcctataa tcggaaacca tgacaattac gatatcgaag tttctgagct tgttgacaag
     4321 ctcaattatc ctgttggtca gttgtgctcc ggtacagttg tttacataat ctgtcctttc
     4381 agcctcagac atgagaaaga agtattcgac tccaaaagcc ctcagcacct tttccacctc
     4441 tgtcctgttc tttatgggaa aacctatagc cgctatgctt tgacccctcc ttatctcccc
     4501 catgccttca agccgtgata taaaagtgcg ctctacaccc agcttatccg ctgcctcctg
     4561 ctgtgacatt ccaagcatgc ggtactgaag aatctcattt attttgttgt ttatcctggg
     4621 aatacttatt attttttccc ctatccttac aagatccata gcaacacctc aaatacactt
     4681 ttttatttat gtgcacaatg ttgtgtacaa tttcatttta gtacttatat attttattct
     4741 caatatggaa atttatccac agcttttcca ataaacggcg gaatttgtgc acaatatatg
     4801 aagaatgcga cg
//
LOCUS       DAOOAB010000091         4300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_23024_length_4300_cov_14.987279, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000091 DAOOAB010000000
VERSION     DAOOAB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23024_length_4300_cov_14.987279"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKU88_12205"
     CDS             complement(<1..818)
                     /locus_tag="PKU88_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HPX70073.1"
                     /translation="MKITEVENGLILEDVKDFDAGHIFECGQCFRWTKEEDGSYTGIA
                     FGKVLNVRSDYEKGIVILDNTNLKDFKEIWFDYFDLGRDYGAIKKELAKDPVLDMAIK
                     HGKGIRILKQEPWELLISYIISANNSIPMISRSVGLLSEMYGRKVQYKGRAYYAFPTP
                     SELQEAGMEELSLCRAGFRCKYIYQAVRMVNMRDIRLEEISSLDIHKAREELMKIPGV
                     GPKVADCIMLFSMQKYEAYPVDVWVKRVTEYFFLGRDVKMKEIGNFAAEKFGDLA"
     gene            complement(946..1752)
                     /locus_tag="PKU88_12210"
     CDS             complement(946..1752)
                     /locus_tag="PKU88_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70074.1"
                     /translation="MEVYALVGQSGTGKSYKAFIVAKDRDIGYIIDDGLLIGGTRVIA
                     GQSAKRERTRIASVRRALFNDPEHRENVKNALKEINPEKLLILGTSLKMIREICQVLE
                     LPELHEILYIEDVSTEKEIELAQKSRREDGKHVIPVPTFEVKKDFSGYFIDSLKIFSK
                     KGRFSGMVEKTIIRPTFSYLGKYELSKAALVQIISICVSKVERINKIMRVRIENKPDG
                     VIIDLDLSIKLITRIDLMIVELQKKLVEEIEYMTGINVLSINVTVKAITT"
     gene            complement(1939..2592)
                     /locus_tag="PKU88_12215"
     CDS             complement(1939..2592)
                     /locus_tag="PKU88_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPX70075.1"
                     /translation="MERFSNISMAVVRRLPKYHRYLGDLLHHDIRRVSSQELSRITGF
                     TASQIRQDLNCFGGFGQQGYGYNVEELYNELGNILGLSRTYRTIIIGAGNVGRAIANY
                     TYFEESGFILYGMFDNSPKKIGDDVKGHKVLPIDEVEDFIKENEIEIAILCTPKEGTQ
                     KIADTLVNCGIKGIWNFAPVDLKLNSDVIIENVHLSESLFTISYLLKEREDLKKKKK"
     gene            2832..>4300
                     /locus_tag="PKU88_12220"
     CDS             2832..>4300
                     /locus_tag="PKU88_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPX70076.1"
                     /translation="MIILSCKNINKSYGIDVILEDVTFTVQENEKVGIVGVNGSGKST
                     LLKIIAGDIEMESGEIFIGRNTSIGYMSQDFSFESNSTVYDELLTVFAPLIQMEKDLH
                     SLELRISGLGSSADKEIAESLLKSYADLQEEYKNSNGFGYKSQIKGVLKGLGFSEEDY
                     GKPVSLLSGGQKTRVALGKILLQNFNLLLLDEPTNYLDIESVEWLEEYLKNLKCTALI
                     VSHDRYFLDMVTDRTFEIENKTLKEYDGNYSKFIEKKQQLREMQLKDYAEQQKEIARQ
                     EAIIARFRQYNREKSIRQAESREKALSRIERIERPDAPVKNIGIKFEPSLRSGNDVLA
                     VENLEKSFDAPLFKNVSFEIKRGEKVALLGPNGIGKTTILKIIAGALKADSGYARLGT
                     NVVAGFYDQEQESLNYNNTVIDEIWDEYPNMNQTELRTKLAAFLFQGEDVFKEISRLS
                     GGERSRVALLKLMLSKTNFLLMDEPTNHLDILSKEVLENA"
BASE COUNT         1200 a         1001 c          804 g         1295 t
ORIGIN      
        1 gccaaatctc cgaacttctc cgccgcaaag ttcccgattt ccttcatttt gacatccctt
       61 cccaggaaga agtactcggt tacccttttt acccacacat ccacaggata ggcttcatat
      121 ttctgcatgg aaaaaagcat tatgcagtcc gctaccttgg ggcccactcc cggaatcttc
      181 ataagctcct ccctggcttt atggatatcc agggaagaaa tctcctccag ccttatatcc
      241 ctcatattta ccattcttac agcctgatat atgtatttgc atctgaaacc tgccctgcac
      301 agggataatt cctccatacc cgcctcctgc agctctgaag gggttggaaa ggcataataa
      361 gccctccctt tatactgaac tttcctgccg tacatttcag acagcaggcc gacgcttctt
      421 gatatcatcg gtatgctgtt gttcgcagat atgatgtatg aaatcaggag ttcccaaggc
      481 tcctgcttca gtatccttat accttttccg tgctttattg ccatatcaag cacagggtcc
      541 tttgccagct ctttttttat tgcaccataa tcccttccca aatcaaaata gtcaaaccag
      601 atttccttaa aatccttaag gttcgtattg tcaagaatca cgatcccctt ttcataatcg
      661 gatctgacat tgagaacctt tccgaaggca attccggtat agcttccgtc ttcttccttt
      721 gtccagcgga agcattgccc gcattcaaat atgtgcccgg catcgaagtc ttttacatct
      781 tcaagtatca agccattctc aacttcagta atcttcatag ccaattctcc tcattcccgc
      841 tccgcgtccc cgcagccctg cccatgtcat tctaagtgta agcaaagaat cttaggactc
      901 ttcactgcgt tcaaagccct tccctaatct ggcctccggc gtctgttacg tcgttatagc
      961 cttcactgtt acgtttatgc ttaatacatt tatacctgtc atgtattcta tctcttccac
     1021 cagttttttc tgaagctcca caatcataag gtcaattctt gtaatcagct ttattgataa
     1081 atcaaggtca atgattaccc catccggctt gttctctatt ctgactctca ttatcttgtt
     1141 tattctctcc acctttgata cacatatgct gattatctga acaagtgcgg ctttagaaag
     1201 ctcatattta cccagataac tgaaggtagg ccttataatc gtcttttcca ccataccgga
     1261 aaacctgccc tttttgctga atatcttaag ggaatcaata aaataacctg agaagtcctt
     1321 tttaacctca aaggtaggca ccggaattac gtgtttaccg tcctccctgc gtgatttttg
     1381 ggccagctct atttcctttt ccgtggatac atcttctata tacagtatct catgcagttc
     1441 gggaagctcc agcacctggc atatttccct gatcatttta agggatgtac ctagtataag
     1501 aagtttttcc gggtttatct cttttaaagc attcttaaca ttttccctat gttcagggtc
     1561 attgaaaagc gctcttctaa ccgatgctat gcgggtcctt tccctttttg ccgattggcc
     1621 tgctataacc ctcgtaccgc ctatcagcaa tccatcgtct attatatatc ctatgtccct
     1681 gtcttttgca actataaatg ccttgtagct tttcccggtg ccgctctgac caacaagcgc
     1741 ataaacttcc acggccacac ccccaacact tttagaatgg tttttcatat aagtattatt
     1801 ctatcatatt tagaagaatt taaaacaaaa aacccgcaaa tataataata cacatttgcg
     1861 agcttctggg cgttcaatct gcaaattcac taaacctgca ccagattaag gcatatatct
     1921 tgcataacct gtacatttct actttttctt cttcttaaga tcctcccgct ctttgagcag
     1981 atatgatatt gtaaacaagc tttcgcttaa gtgaacattc tcaattatga cgtcactgtt
     2041 tagcttaagg tcgacaggag caaaattcca tatccccttt ataccacaat tcaccagtgt
     2101 atctgctatc ttctgcgttc cttccttggg tgtgcataga atagctatct ctatttcatt
     2161 ttctttaatg aagtcttcta cctcatcaat gggcagaact ttgtgacctt ttacatcatc
     2221 ccctattttt ttggggctat tgtcaaacat accatacaaa atgaagccgc tttcttcaaa
     2281 gtatgtgtaa tttgctatag ctcttcctac attacctgcg cctattataa tagttctgta
     2341 tgttctgctc agtccaagta tatttccaag ctcattatac aattcttcca cattgtagcc
     2401 atatccctgc tgtccgaagc ctccaaagca attcaagtcc tgtcttatct gggaagcggt
     2461 aaaacctgtt atcctgctaa gctcctgcga tgatactctc cgtatgtcat ggtgcaataa
     2521 atctcctaga tatctgtgat acttgggaag acgtctgacc actgccatgg atatgttgct
     2581 gaatctctcc atactatcta cccctaactt ttaaaattga atcaactata tggtaagaag
     2641 ttaataaata gattatatta tattacttac tttattgttt ggtaaattta ttttattata
     2701 tactgatatt gttgtcaaga aattacagat attaccacat tcttgaatga tattgaacta
     2761 tgtacaaatc ctgttcactt ctgatagaat aattattgtg ctatggatat atcaccggcg
     2821 gaggttattc tatgataatt ctttcttgta aaaatataaa taaaagctat ggaatagacg
     2881 ttattcttga agatgttacc tttacagtgc aagagaatga aaaagtcggc atcgtaggcg
     2941 taaatggatc aggaaagtca accctcctca agattattgc cggcgacatc gaaatggagt
     3001 ccggggaaat attcatcggc aggaatacca gcatcggata catgtcccag gatttcagct
     3061 ttgaatccaa tagcacagta tatgatgagc tgctgactgt ttttgccccg cttattcaaa
     3121 tggaaaagga ccttcactcc ctggaactca gaatcagcgg tctgggaagc tccgctgaca
     3181 aggaaattgc agagtccctg cttaagtcct atgcagattt gcaggaagaa tacaaaaaca
     3241 gcaacggttt tggctataaa agccaaataa agggtgtgct taaagggctg ggcttctctg
     3301 aggaggatta cggcaagcct gtttcattgc tcagcggtgg gcaaaaaaca agggttgccc
     3361 tgggtaaaat ccttctgcag aattttaatt tgctgcttct ggacgaaccg accaattatc
     3421 ttgatataga gtctgtggag tggctggagg aataccttaa gaaccttaaa tgtacggcgc
     3481 ttatagtatc ccacgaccgc tacttcctgg acatggtgac tgacaggacc tttgaaatcg
     3541 aaaacaaaac ccttaaagaa tatgacggca attactccaa attcattgag aaaaagcagc
     3601 agctcagaga gatgcagtta aaggattatg cggagcagca aaaggaaatc gcccgtcagg
     3661 aagcaataat agccagattc aggcaatata acagggagaa gagcataagg caggcggaaa
     3721 gcagggaaaa agccctcagc cgtattgaaa ggattgagag gcccgatgcc cctgtaaaaa
     3781 atataggtat caaatttgaa ccgtccctta gaagcggtaa tgatgtcctt gctgtggaaa
     3841 acctggagaa atcctttgat gcaccgctct tcaagaatgt gagctttgaa attaaacggg
     3901 gcgaaaaggt tgcgctgctg ggccctaacg gaattggcaa aactaccatc cttaagataa
     3961 tagcaggcgc attaaaggcg gattccggct acgcaagact tgggaccaat gttgttgcag
     4021 gcttctatga ccaggagcag gaaagcctga attacaataa tacagtaatt gatgagatat
     4081 gggatgaata ccctaatatg aaccagacag aattgcgtac aaaactggct gcatttctgt
     4141 tccagggcga ggatgtattc aaggaaatca gcagactcag cggcggtgaa agaagcaggg
     4201 tcgccctttt gaagcttatg ctgtccaaaa ccaatttcct gcttatggat gagcctacca
     4261 accatttgga cattttatca aaggaagtac tggaaaatgc
//
LOCUS       DAOOAB010000092         4035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_24944_length_4035_cov_13.255276, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000092 DAOOAB010000000
VERSION     DAOOAB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24944_length_4035_cov_13.255276"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1277
                     /gene="pyk"
                     /locus_tag="PKU88_12225"
     CDS             <1..>1277
                     /gene="pyk"
                     /locus_tag="PKU88_12225"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPX70077.1"
                     /translation="CSITYKELPDEVAVGSRILIADGVIELRVLDKNETDVICRVVNG
                     GTLGDRKNVNIPGATSKLPAITDKDVADLVFGIENEVDFIAASFIRKAADVLEIRKIL
                     EENAGGHIQIIAKIENQEGVDNVKDILRVCDGLMVARGDLGVEIPTEEIPLTQKLLIK
                     RANEAGKPVITATQMLDSMMRNPRPTRAEVTDVANSIIDGTDAIMLSGETAAGKYPVE
                     TVLTMSSIAERIERELDYGKSLSSFSGRRITIANAISHSSCTTAFELQAAAIITPTQS
                     GSTARMVSKFRPKAPIIATTTNAGVMRKLCLSFGVYPIYVPFTERTDDIIDRAVDAAV
                     ESKLIKNGDLVVITAGIGAAGSTNLIKVHTVGEILSKGIGIGDRSVTGKVSIVDDIPG
                     DFGKLREGDILVARSTDIEMIPLMEKASAFIVE"
     assembly_gap    1278..1377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1378..1734
                     /locus_tag="PKU88_12230"
     CDS             <1378..1734
                     /locus_tag="PKU88_12230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPX70078.1"
                     /translation="YYIYFEMGRTEYLREAGGISYKEMEELGIMLPLTETHCKYRIPA
                     VYDDEILVRTGIKEMTVARIAFSYKLLRTSDGALLAEGETVHAFANRSGRPINIKKHC
                     SNLYSSLYELAKDKEE"
     gene            1738..2745
                     /locus_tag="PKU88_12235"
     CDS             1738..2745
                     /locus_tag="PKU88_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPX70079.1"
                     /translation="MQYRMLGKTGMKVSGISLGGIPLQQLNQDDANEFIEKALEQGIN
                     FIDTARGYTVSESLIGGALSGKRQQVFLATKSMARDREGFKKDIEISLNNFKTDYIDL
                     YQFHNIVSLQELEKAMAKGGAIEAMLEYKDKGIIKSIGITGHSLEVIEKAIEYDVFET
                     IQFPYNAVETQGTELFRKAYEKNLGTIAMKPFAGGAITNARASLKFILENQHITCAIP
                     GMFSIEQLMDNIKAIEEMPLSQDEKEALSKEASELGERFCRRCGYCQPCPKGINIPVV
                     LVMESYYKRYNLMEWAQTRYNNMPSRASDCEKCGLCEAKCPYQLPIREMLEAVKQTFG
                     Q"
     assembly_gap    2928..3027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3064..4023
                     /locus_tag="PKU88_12240"
     CDS             3064..4023
                     /locus_tag="PKU88_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX70080.1"
                     /translation="MKLKADDIAGISGHQQIAYNVELITKRIREYYLEEDINALEKLR
                     DLCKNEGMMIEAAKMHRNIGMYHFYMNNCSNAVKSMKLAIDTVRREDCLNLLVEYYSE
                     LGYMYFYNHEYIRSVEYYEKAEELLLQVSEMGREISYLHYYRYGILLSNMQDYYYSEQ
                     KLKNALLYSGDDKDTALIIMNIGLLFKRQKDFKAALRYYSKALCLADQKDMRVKSKVY
                     NNIAIVYKILGQYKKALSYIDKAFKCIEDDDMAMRFICFNTYTEIKLLMGEKESVLDD
                     FLELLLKVEDIHLYKGYIIQGISNMIVIGSVNCKSKCNPIDSN"
BASE COUNT         1323 a          617 c          940 g          955 t
ORIGIN      
        1 tgcagcatta catacaaaga actgcccgat gaagttgctg tcggctccag aatccttata
       61 gcagatgggg tcatagagct tagggttctg gataaaaatg aaaccgatgt catatgccgg
      121 gtggtaaacg gaggtactct cggagacagg aagaatgtga atattccggg agctacttcc
      181 aagctgcctg ctattactga taaggacgtc gccgaccttg ttttcgggat agaaaatgaa
      241 gtggatttta tcgcagcttc atttataaga aaagccgccg atgtgctgga gataagaaag
      301 attctggagg aaaacgccgg ggggcatatt cagataattg cgaagataga aaaccaggag
      361 ggtgttgata atgtaaagga tatcctcaga gtatgcgacg gccttatggt tgcaagaggg
      421 gaccttggag ttgaaattcc cactgaggaa atacctctga ctcaaaagct tctgattaag
      481 agagccaatg aagcgggcaa gccggttata acggcaacac agatgctgga ctctatgatg
      541 cggaatccca ggcctacgag ggcagaagtg acggatgtgg caaattcaat aatagacggt
      601 acggatgcaa taatgctgtc gggagagaca gcagcgggga aataccccgt agaaaccgta
      661 ctgaccatgt catcaattgc cgagagaata gaaagagaac tggattacgg gaaaagccta
      721 agcagctttt caggaagaag aataacaata gcaaatgcca ttagccacag ctcatgcact
      781 actgcatttg agcttcaggc agctgccatt ataacaccta cacaatcggg ttctacagca
      841 aggatggtat cgaaattcag gccaaaggcg ccgataattg ccactaccac taatgccggt
      901 gtaatgagaa agctttgcct gagcttcggg gtttatccca tatatgtgcc ttttaccgag
      961 aggacggatg atataattga ccgggctgta gatgctgctg ttgaatccaa gcttataaag
     1021 aacggcgatc ttgtggttat aacggcaggt atcggagcgg caggcagcac aaaccttatt
     1081 aaggttcata cagtaggaga gatactgtca aaaggaatag gaataggtga caggtctgtt
     1141 accggaaagg tttcaattgt tgatgatata cccggagatt tcggcaagct ccgggaagga
     1201 gatatattgg ttgcaagatc tactgatata gagatgatac cccttatgga aaaagcatcg
     1261 gcatttattg tggaagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat
     1381 tacatatatt ttgaaatggg aaggactgag taccttcggg aggcaggagg gatatcatac
     1441 aaagaaatgg aagaactggg gataatgctt ccgttgacag aaacccattg caaatataga
     1501 attcccgcag tgtatgatga tgaaatcctg gtcagaaccg gtataaagga aatgaccgtt
     1561 gcaaggatag ctttctccta caagcttttg aggacttcag acggagcatt gctggcggaa
     1621 ggagaaacag tccatgcctt tgccaacaga agcggaaggc caataaatat aaaaaagcat
     1681 tgcagcaatc tttacagctc attgtatgag ctagcaaagg acaaggagga ataatacatg
     1741 cagtacagaa tgcttgggaa aacaggaatg aaggtatcgg gaataagcct tggaggcata
     1801 ccccttcaac agctgaatca ggatgatgca aatgagttta tagaaaaggc attggaacag
     1861 ggtataaatt tcattgatac cgcaagggga tacaccgtaa gtgaaagcct gataggaggc
     1921 gcactgtcag ggaaaaggca gcaggtgttc ctggctacaa aatccatggc aagggatagg
     1981 gaaggattta agaaggatat agaaatctct ttaaataatt tcaaaacaga ttatattgat
     2041 ctttaccaat tccataatat agtcagcctg caggagttgg aaaaagcaat ggcaaaaggc
     2101 ggagcgattg aggccatgct tgaatacaag gataaaggaa ttataaaaag catcggaata
     2161 acaggccata gcttggaggt tattgaaaag gccattgaat atgatgtttt tgaaacgatt
     2221 caattcccat ataatgcagt tgaaactcag ggaacggagt tgttcagaaa agcatatgaa
     2281 aaaaatcttg gaactattgc aatgaagcca tttgcgggag gtgccattac aaatgccagg
     2341 gcatcattga agtttatact ggagaatcag catataacct gtgcaatacc cggaatgttc
     2401 agcatagaac aattgatgga taatataaag gcaatagagg aaatgccgtt gtcacaggat
     2461 gaaaaggaag ccctttcaaa ggaggcctcc gaattgggag aacgcttttg caggagatgc
     2521 ggttattgcc agccctgccc caaggggatc aatatacctg tagtattagt tatggaaagc
     2581 tattacaaga ggtataatct tatggagtgg gctcaaacaa gatataataa tatgccttca
     2641 agggccagcg actgtgagaa atgcgggtta tgtgaggcaa agtgcccgta tcagcttcca
     2701 atcagagaaa tgcttgaagc cgttaaacaa acctttgggc agtaaaaatc gaaaaaaatg
     2761 tggtcaaaag gccacttttt ttatttgcgt atatgagttg cagagggatg tcgaaaaagc
     2821 gcagtttata taaaatataa gacaattcag cgtaaaacac tgtaaagtaa aagtaaaaca
     2881 ctgtaaagta aaagtaaaac actgtaaagt aaaagtaaaa cactgtannn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnata tttttgataa attggttaat ggaggtgagg
     3061 ctgttgaaat taaaagcaga tgatattgca ggaatatcag gtcatcagca aatagcatat
     3121 aatgttgaat taatcacgaa gagaataaga gaatattatt tggaagaaga catcaatgca
     3181 ttggaaaagt taagggatct gtgcaaaaat gaaggaatga tgatagaagc tgcaaaaatg
     3241 cacaggaaca ttggaatgta tcacttttat atgaataatt gcagtaatgc cgtaaaatca
     3301 atgaaacttg ctatagatac agtcagacgc gaagattgtt tgaatcttct tgttgaatat
     3361 tactctgaat taggatacat gtatttttat aatcacgaat atatacgctc cgtagaatat
     3421 tatgagaagg ccgaagaact gcttttgcag gtttcggaaa tgggcaggga aatatcatat
     3481 ctccattatt acagatacgg gatacttctg agcaatatgc aggattatta ttattccgag
     3541 cagaagctga aaaatgcatt gttatactcg ggggatgata aggataccgc acttattatt
     3601 atgaatatcg gcctgttgtt taagaggcag aaggatttta aggcagcact cagatattac
     3661 agcaaggctc tttgcctggc agatcaaaag gatatgagag taaagagcaa agtatataac
     3721 aatattgcta tagtttataa aatactgggg cagtataaaa aggcactcag ttatattgat
     3781 aaggcattta aatgtattga agatgatgat atggcaatgc ggttcatatg ctttaacaca
     3841 tatacggaga ttaagcttct gatgggagaa aaggaatcgg tactggatga ttttttggag
     3901 cttcttttaa aggtagaaga tatacatcta tataaaggct atataataca aggtataagc
     3961 aatatgattg taattggaag cgtcaattgc aaatcgaaat gcaaccctat cgattcgaat
     4021 tgaactttat taagc
//
LOCUS       DAOOAB010000093         3952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_25594_length_3952_cov_17.359764, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000093 DAOOAB010000000
VERSION     DAOOAB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25594_length_3952_cov_17.359764"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1134
                     /locus_tag="PKU88_12245"
     CDS             139..1134
                     /locus_tag="PKU88_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HPX70081.1"
                     /translation="MTKALALISGGLDSILAAKLIQMQGIEVIGVCFASAFFGSKNAE
                     KMAAQIDMPLIVVDFTEEHLKMTKDPKHGYGKNMNPCIDCHAMMLNYAGKMLEEYGAS
                     FLITGEVLNQRPMSQNKKSLGIVQKESGFEDKILRPLCALNLPPTKMEIDGLVERSKL
                     LGISGKSRKQQIELARQWGIKDYPSPAGGCMLTEPQFANRLRDLYSHDKEAVGPMDIK
                     LLRIGRHMRISPSARIVCTRNEGEYEPLMELVKEEYLIFDTADCSGSTVVLIPSGAGV
                     LSDEDVLFAASVAARYSKEREKDSVRVKFKKKKDSEYEYIQVKPAEDEDIKKVLL"
     gene            1301..2716
                     /locus_tag="PKU88_12250"
     CDS             1301..2716
                     /locus_tag="PKU88_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPX70082.1"
                     /translation="MNSLFKENIEDILDNIEEGIHIVDSSGIIVYYNRFAAKLDNINP
                     EEAIGRHILEIYPSLTEETSTILRVIKTGRPILNYEQTFKNYKGIQITTINSTIPIIS
                     GKRVMGALEVSRDITEVKKLSLKVVDLQAELMKDARPEKSYDGNKAFFNFADIIGISE
                     AMLKLKKDAMAVASSPSPIMVYGETGTGKELLLHAIHNASLRRDKPFIAQNCAALPET
                     LLESILFGTVKGSFTGAENRPGLFEIANGGTLFLDEINSMSLQLQAKLLRVIQDGNVR
                     RVGDTKTIHVDVRIVTAINIDPRQAVVDKTLRDDLFFRLSVISLKVPPLRERKGDIHF
                     LIEHFIKKYNKRLHKNVSHVSSSVEEFFLNYAWPGNVRELEHAIEGAMNITEGDVIES
                     KHLPHYLGEIFSKFDFTQEDHEIKSLKEALYNLERSLIEKALKRCEGNVTRAAELLDI
                     PRQTLQYKISKYIIKADNAGF"
     gene            3078..>3952
                     /locus_tag="PKU88_12255"
     CDS             3078..>3952
                     /locus_tag="PKU88_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPX70083.1"
                     /translation="MKKGCKYGTHRVIEPAGVLPQPANKVDNNMDVLYDNEILIDVHT
                     LNIDSASFTQIEAQAGGDEKKIAEIMLDIVEKQGKHRNPVTGSGGMLIGTVEKIGPAL
                     EGKTDLKVGDKIATLVSLSLTPLRIDKIKEIRKEIDQVDIEGKAILFESGIYAVLPKD
                     MPEKLALSALDVAGAPAQTAKLVKPGDTVFIIGAGGKSGVLCSYEAKRRAGITGKVIG
                     CCGSPKSADRLMKLGFCDEVFVGNAKMPVEIMNKVSEYTNGQMADITINCVNIPDTEM
                     TSILATKNTGVVYFF"
BASE COUNT         1277 a          760 c          979 g          936 t
ORIGIN      
        1 ccgcagtatt atctgaccgt ctcaacagct tcggcgtttc tttcccacat gacaagtacc
       61 ggtatgctta ccacgttatt tgaagataat aaacttaaat ttaagagagc gtaagaactg
      121 gaaaggattg atatacatat gaccaaagct ttggccctga tttccggggg gcttgacagc
      181 atattagccg caaaactgat tcaaatgcaa gggattgaag taattggagt gtgctttgcc
      241 tctgcgtttt tcggctccaa aaatgccgaa aaaatggcgg cacaaataga tatgccttta
      301 atagtagttg attttaccga agagcatctg aaaatgacaa aggatcccaa gcatggatac
      361 ggaaagaata tgaacccttg catcgactgc catgcaatga tgctgaacta tgcggggaaa
      421 atgcttgagg aatatggtgc aagtttttta atcaccggag aagtacttaa ccagaggcca
      481 atgtcccaga ataagaaatc cctgggcata gtgcaaaagg agtccggctt tgaagacaag
      541 atactgaggc ccctttgtgc tctgaatctc ccgccgacga aaatggaaat tgacggcctt
      601 gttgagagga gcaagcttct cggtatcagc ggcaaatcaa gaaaacagca gatagagctg
      661 gcaagacagt gggggatcaa ggattaccct tcccctgccg gaggctgcat gcttaccgaa
      721 ccgcagtttg ccaaccgtct gagagatttg tacagccatg acaaggaagc ggtggggcca
      781 atggatataa agctcctgag aatcggcagg catatgagga tatccccttc tgcgcggatt
      841 gtatgcacca ggaatgaagg tgaatatgaa ccattaatgg aacttgtgaa agaagaatat
      901 ctcatatttg atactgcgga ttgcagcggc tccacagtcg ttctgatacc ctccggggca
      961 ggggtgttgt ctgatgagga tgttttgttt gctgcttccg ttgcagccag gtatagcaaa
     1021 gaaagggaaa aggacagcgt aagggtgaaa ttcaaaaaga aaaaagacag tgagtatgaa
     1081 tacatacaag taaaacctgc cgaggacgaa gacataaaga aggtgctgct gtagattcat
     1141 gaattttcta aaaattcaac agattatttg taattttctg tcgggtggta tatacttaag
     1201 atatagcagg taatcaacct gctttttata caagcctcat gccgaataat cggcagtcgg
     1261 caaacaggcg gaagaggatt taagaaggag tggaagcttg ttgaattctt tattcaagga
     1321 gaacattgaa gacatattgg acaatataga agaaggcatt cacattgttg acagctcggg
     1381 catcatagtg tattacaaca gatttgcagc caaattggac aatattaatc cggaggaagc
     1441 aataggcagg cacatactcg aaatatatcc ctccctgaca gaggaaacca gcacaatact
     1501 gagggttata aaaacgggaa ggcccatact taactatgag cagaccttta agaactacaa
     1561 aggcattcag ataacaacca taaattcaac catacccatt atttcgggga agcgggttat
     1621 gggtgctctt gaagtatcga gggacataac ggaggtaaag aagctttcct tgaaggttgt
     1681 ggatttgcag gcggagctta tgaaagatgc aaggcctgaa aagagctacg acggaaataa
     1741 ggcattcttt aattttgcag atataatcgg aatatcggaa gcaatgctca agctcaagaa
     1801 agatgccatg gcagtagcct catctccttc accgattatg gtttatggag agacgggcac
     1861 aggaaaggaa ttgctccttc atgccataca taatgcaagc ctccgcaggg acaaaccttt
     1921 cattgcccaa aattgtgcgg ctcttccgga aaccttgctg gaaagcatac ttttcggtac
     1981 ggtaaaaggc agcttcaccg gagcggaaaa ccgcccgggg ctttttgaaa tagccaatgg
     2041 cggtaccctg ttcctggatg agatcaattc aatgtctctt caactgcagg caaagctgct
     2101 gagggtaata caggacggca atgtgagaag agtcggcgat acaaaaacta ttcacgttga
     2161 tgtcagaata gtgacggcta taaatattga tcccaggcag gcggttgtgg ataagactct
     2221 aagggatgac ctgttcttcc gtctgagcgt aatatctctg aaggtgcctc ctttaagaga
     2281 aaggaagggt gacatacact tcctgataga gcattttatt aagaaataca acaaacggct
     2341 gcataagaat gtcagtcatg taagcagcag cgtggaggag ttttttctga actatgcatg
     2401 gccggggaat gtcagggaac tggagcatgc catagaagga gccatgaaca taacagaggg
     2461 ggatgtaatc gagagtaagc accttcccca ttacctggga gagatatttt caaaatttga
     2521 cttcacccag gaggatcatg aaataaagtc cctgaaggaa gccctgtaca acctggaaag
     2581 aagccttatt gagaaagccc tgaaaaggtg cgaaggcaac gtaacaaggg cagcagagct
     2641 gctggacata ccaagacaga cacttcaata caaaataagc aagtatatca tcaaagctga
     2701 caatgccgga ttttaggcat aaatttatta atcgctgaca tattgataaa taggggcagt
     2761 atatataggc ttagaatact tagacagaaa ttgttaatgg ctcttatgaa acttggctta
     2821 gtaataaccg tatgcgaggc atggacgccg agcaatctgt cacggtcacg gatgaccttt
     2881 gacagagtta ggtttttctt agtcaagttg aattagtgtc atcaattttt gtctactatt
     2941 cgagactata tatactgtcc atatattaaa tatgtcagca attaatattt tcttcgcaaa
     3001 cctgtaacct ccgattggca tactatttgc ttattagaat tattacaagc ccaaaaatta
     3061 aggaggaata atcgcacatg aaaaagggat gcaaatatgg tacccatagg gtcattgaac
     3121 cggcaggagt cttgccgcag ccggcaaaca aagttgacaa caatatggac gtactttatg
     3181 acaatgaaat acttatcgat gtacataccc tgaacataga ctccgccagc tttacgcaga
     3241 tagaagcaca ggccggcgga gatgaaaaaa agatagcgga aatcatgctg gatatagtag
     3301 aaaagcaggg caagcacaga aaccctgtaa caggctccgg cggaatgctt atcgggactg
     3361 tggagaagat tgggccggca ctggaaggga agactgacct gaaggtcggg gataagatag
     3421 caacacttgt atcgctttca cttactcctc tcaggataga taagatcaaa gaaatcagaa
     3481 aagaaataga tcaggttgat atagaaggaa aagcaatact ttttgaaagc ggaatttatg
     3541 cggtgctgcc gaaggacatg ccggaaaagc tggcactgtc agctcttgac gtagccggtg
     3601 ctcctgccca gactgcaaag ctggtgaaac ccggtgacac agtattcata ataggtgcgg
     3661 gaggaaaatc aggagtgctt tgcagctatg aagcaaagag aagagccgga ataacgggca
     3721 aggtcatcgg ctgctgcgga agcccgaaga gtgcagacag gctgatgaag cttggattct
     3781 gtgatgaggt ttttgtcgga aatgcaaaaa tgcccgtgga aataatgaac aaagtatctg
     3841 aatatacaaa tggccagatg gctgatatca caataaactg tgtaaacata cccgatacgg
     3901 agatgacaag catacttgcg acaaaaaata ccggagtggt atatttcttc ag
//
LOCUS       DAOOAB010000094         3813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_26844_length_3813_cov_14.452368, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000094 DAOOAB010000000
VERSION     DAOOAB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26844_length_3813_cov_14.452368"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PKU88_12260"
     CDS             complement(<1..1061)
                     /locus_tag="PKU88_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX70084.1"
                     /translation="MDRRIVIAGGGIAGLNAAKSAREQDPDCSIVILEALNTNTYVRT
                     RLPDYISGTASYKEIFPYDDSWYEKNRINLKKDTRITGIDAAHKTIITDKGNYDFDSL
                     VIALGSSGNIPPIPGAKLENCFPVRTVADADKIRSLSCGGKICTIIGGGLLGLEMAWA
                     IRQLGCDVNVIHNSGRLLPKQIDEEGAKLLYKAISNKGINLYMNAQVQEIGGENRAEY
                     VRLDSGTVVKSDFVILSVGVNANTQPLKDSGINMGRSVAVDKYMETNIEGIYAAGDVA
                     EYNGKCYGIWPIAVEQGKIAGTNAAGGKLEYSEIHPFTSLKIKGITMFSIGDVFSEDS
                     LSIAEADFESGRYIKLLIK"
     gene            complement(1107..2507)
                     /locus_tag="PKU88_12265"
     CDS             complement(1107..2507)
                     /locus_tag="PKU88_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX70085.1"
                     /translation="MNEAENDKRERKNFLNIKGKADAEVMRIAWPVLAELLLGSLFGM
                     VDMMMLGRISDNALAAASVAAVGMTNQPLFIGISLVQALNIGGTAMIARYFGANQNEK
                     IEITLKHVMLLSMLMLAIPLSILGFVFTDSIMKLMGAQADTLQVGRIYFRIIMVGYVF
                     QSLNMSISAALRGIGETRVPMIINLRCNFLNVIGNAILIYGLLGFPRLGITGAGISTA
                     LSNAVASVFIFGYVLKGKSVVRFSIKKPFNLNRRILYNLFKIGMPASFEQLVLRTGVL
                     LFVRIVAELGTVIYAAHQIALSILGLSFQPGQAFGIAASSLVGISLGAGKPDMAEEYA
                     RKTRKMGSIISTFMAIIFFFFGPQLVGLYSNDPMIIKNSSVALKIIALVQPFQSSQFI
                     LAGALRGAGDTFWPLVSTSVGVLMVRVVLAYIFVSVMGYGLMGAWIGVLVDQLVRWMF
                     VYFRFKTGKWKYIKIR"
     gene            complement(2631..3788)
                     /locus_tag="PKU88_12270"
     CDS             complement(2631..3788)
                     /locus_tag="PKU88_12270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPX70086.1"
                     /translation="MINPFEYVLPTKIRYGAGMLKVLGEELRLLKAKKVMIITDKGLV
                     NAGMIKKAAEIIEAEDIDFIVYDEIEANPKDYNVEACAEKARAESVDTLLAFGGGSPI
                     DAAKATAVLARQGGKVRDYQGKGRIKDDCLPLITIPTTAGTGSEVTFSSVITDTKEKF
                     KFTIKSTAIAAKTAIIDPELTLSVPPLITAATGIDALTHAIEGYTANCTEPIAEAAGL
                     YAVEYIAGNIEEAVKNGRNIAARDKMMIGSLLAGLSFSHADVASVHCMAEALGSMYDA
                     PHGMCNAILLPYVMEYNLPAAGHKYARVARAMGIEEKDDYTAAVKGIEHIRKLSKEIG
                     LPGIRSLNVNTDDFELLAEMSEKNGSNDSNPRKISKDEYVKLFYKAYNDCN"
BASE COUNT         1025 a          967 c          604 g         1217 t
ORIGIN      
        1 tcctttatca aaagctttat gtatcttccg ctttcaaaat cagcttctgc aatcgaaaga
       61 gaatcttcgg aaaacacatc tccgattgaa aacattgtta tcccctttat cttaaggctt
      121 gtaaagggat gaatctccga atactctaat ttccctcccg ctgcatttgt tccggcaatt
      181 ttaccctgtt caaccgctat aggccatatg ccatagcatt tgccgttata ttcagctaca
      241 tcacctgccg catatattcc ttcaatattt gtttccatat atttatcaac tgctacagac
      301 ctgcccatat ttatccctga gtctttcaaa ggttgtgtat ttgcattgac tcccacagac
      361 aatatgacaa aatcggattt gactactgtt ccgctgtcaa gcctgacata ctccgccctg
      421 ttttctcccc cgatttcctg cacctgtgcg ttcatataca gattaatacc tttattagat
      481 attgccttgt ataatagttt tgctccttcc tcatcaatct gctttggaag cagcctgcct
      541 gaattatgta taacattgac atcacagcca agctgcctta ttgcccaagc catttcaagc
      601 cccaaaagcc ctccccctat tattgtgcat atttttccgc cgcaggacaa gcttcttatc
      661 ttatcagcat cagccacagt acgcacagga aaacagtttt ccagttttgc tcccggaata
      721 ggaggaatat tgccgctgct ccctagtgca ataaccagcg aatcaaaatc ataattcccc
      781 ttatctgtta ttatggtttt atgtgcagca tcaattcctg taatcctggt gtccttttta
      841 aggtttatcc tgttcttttc ataccagctg tcatcataag ggaatatttc tttgtatgaa
      901 gcagtaccgg atatatagtc gggaagtctg gttcttacat atgtatttgt attcaaagcc
      961 tccagtataa caatactgca gtccggatcc tgttcccttg ctgactttgc agcattcaat
     1021 cctgcaattc cgccgccggc gattactatc cttctatcca tattaaaact cctttcatta
     1081 ataccataat tgaccattat tcatatttat cttatcttaa tatatttcca ttttcccgtt
     1141 ttaaacctga aataaacaaa catccatctg acaagctgat ctaccaatac gcctatccac
     1201 gcccccatca ggccgtagcc cattacactg acaaatatat acgctaacac aactcttacc
     1261 atcaatacac caacagaagt ggataccaga ggccagaagg tatccccagc acccctgagg
     1321 gcccctgcca atatgaattg agatgactgg aagggctgaa ccagtgcaat tatcttcaat
     1381 gcaacggaag aattcttgat aatcatcgga tcattggaat ataaccctac aagctgcggt
     1441 ccgaaaaaga aaaatattat agccataaat gtagaaataa ttgaccccat ctttcgggtt
     1501 tttctggcgt attcttccgc catatcaggt ttccctgcac ccagggatat ccctaccaaa
     1561 gatgacgctg ctattccaaa ggcctgtccc ggttgaaagg aaagtcccaa tatactcaga
     1621 gcaatctgat gggctgcata aataacggtc cccaattcag caactatcct gacaaaaaga
     1681 agtacacctg tccttaagac caattgctca aaggatgccg gcatacctat tttgaataaa
     1741 ttatataaaa ttctcctgtt tagattaaag ggcttcttga tgctgaatct tacaacgctc
     1801 ttacctttta agacatatcc gaatataaat acacttgcaa ccgcatttga taatgctgta
     1861 gagatgcctg ctcccgtaat cccaagtctt ggaaatccca gcaagccgta tattaatatt
     1921 gcattgccta taacatttaa gaaattgcat ctcaagttta ttatcatcgg cactcttgtt
     1981 tcgcctattc ccctcagtgc cgctgatatg gacatattaa gagactgaaa tacatatccg
     2041 accattatta tcctgaaata gattctcccc acttgcaggg tatctgcctg ggcacccatc
     2101 aatttcataa tggaatctgt aaagacaaac cccaatatag acagaggtat tgccagcatc
     2161 agcatgctta gaagcattac atgcttaagg gttatttcta tcttctcatt ctgattggcg
     2221 ccgaaatacc tggcaatcat tgctgtcccg ccgatattca gagcctgaac taaagatata
     2281 cctataaaca gcggttgatt tgtcatgcca acagcagcta cggaagcagc tgccaaagca
     2341 ttatcactga ttcttcccag catcatcatg tctaccattc cgaataatga ccccaaaaga
     2401 agctctgcca gtaccggcca tgcaatcctc ataacttcgg catcggcctt gccctttatg
     2461 ttcaagaagt ttttccgttc tcttttgtca ttctctgctt cgttcatttt aatgattcct
     2521 ttttaaatat caatattaat actattatat cataaatccg caaggattta tgatagccca
     2581 tgtatcaaga taaaagcagc cggaacaatc cccactgctt tccgcaatca tcagttgcag
     2641 tcgttatatg ctttataaaa cagttttaca tattcatcct tgcttatctt ccttggatta
     2701 ctgtcattag agccattctt ttcggacatt tccgccaaca gctcgaaatc atcggtattg
     2761 acatttaaag accttattcc cggcagccct atttcctttg acagctttct tatatgctcg
     2821 atacctttca ctgccgccgt gtaatcatct ttttcttcta tacccattgc ccttgctacc
     2881 ctggcatatt tatgccctgc ggcaggcagg ttatattcca tcacatatgg aagcaatatg
     2941 gcattgcaca ttccatgggg cgcatcatac atgcttccca gtgcttctgc catgcagtgt
     3001 actgaagcta catcggcatg actgaaagat aagcctgcca gcaggcttcc aatcatcatt
     3061 ttgtctcttg cggctatatt tctcccgttc ttaaccgctt cttcaatatt gcctgctatg
     3121 tattcaactg catacaagcc tgctgcttct gcaataggtt cggtacaatt tgccgtgtaa
     3181 ccttctatag catgagttaa ggcatctata cccgttgcag ctgttatcag cggcggtacg
     3241 cttaatgtca gctcaggatc tattatggct gttttggctg ctattgccgt actttttatt
     3301 gtaaatttga atttttcctt agtatccgta ataactgaag aaaaggttac ctcacttccc
     3361 gtaccggctg tggtaggtat agttatcaaa ggcaaacagt catcttttat tctaccctta
     3421 ccctgataat ccctaacctt tcctccttgt cttgctaaaa ctgccgttgc tttagccgca
     3481 tctatagggc ttccgccgcc gaaagccaac agcgtatcaa ctgattcagc cctggctttt
     3541 tcagcacatg cttctacatt ataatccttt ggattagctt cgatttcatc gtaaactata
     3601 aaatctatat cttcagcttc gattatctct gcagcctttt ttatcattcc cgcattaaca
     3661 aggcctttat cggttattat cattactttt tttgctttca ataatctcaa ttcttctccc
     3721 aatactttca gcatacctgc cccatacctt atcttagtcg gcaatacata ttcaaacgga
     3781 tttatcatat ttattccctc cttaattcat taa
//
LOCUS       DAOOAB010000095         3550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_29358_length_3550_cov_17.891559, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000095 DAOOAB010000000
VERSION     DAOOAB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29358_length_3550_cov_17.891559"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKU88_12275"
     CDS             complement(<1..317)
                     /locus_tag="PKU88_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX70087.1"
                     /translation="MEFLRYTHLLYFLATWIYVFIFIKPKRIKELIPVAVLGFIVLST
                     VDFYIVSLGLYQYNKPLFNIFGAPLFHLLWGGGAAIVYMHYMKQGFTRKFVNVVLFTM
                     ITLA"
     gene            complement(443..2467)
                     /locus_tag="PKU88_12280"
     CDS             complement(443..2467)
                     /locus_tag="PKU88_12280"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPX70088.1"
                     /translation="MVKNYEISEAMTVKLDNRLPDMPKFAEGIRRAPNRGFTLTPDQA
                     EIALKNALRYIPEELHEKLAPEFMEELVTRGRIYGYRFRPEGRIYGKPIDEYKGNCIE
                     GKAFQVMIDNNLDFEVALYPYELVTYGETGQVCQNWMQYRLIKKYLEVMTDHQTLVVE
                     SGHPLGLFHSKPTDPRVVITNALMVGMFDNQKDWHYAMQMGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRKKLGIAPEEDLRGYLFVTSGLGGMSGAQPKAAEIANGVGIVA
                     EVDYSRIETRLNQGWISRVSSDLKEVFSIAEDYLKKKDKISIAYHGNIVDLLEYAVNN
                     DIKIHLLSDQTSCHAAYDGGYCPQGLTFEERTRMLKEDREQFCKLVDKSLRHHFQLIR
                     KLADKGTYFFDYGNSFMKAVYDAGAKEIAKNGVDEKDGFIFPSYVEDIMGPELFDYGY
                     GPFRWVCLSGKHEDLIKTDHAAMECIDPARKAQDRDNWVWIRDAEKNKLVVGTQARIL
                     YQDAWGRTKIALKFNEMVRKGEIGPVMLGRDHHDVSGTDSPFRETSNIKDGSNIMADM
                     AVQCFAGNAARGMSLVALHNGGGVGIGKAINGGFGLVLDGSERVDEIIKDAMLWDVMG
                     GVARRSWARNENSVTTSIEYNKSQEGKDHITLPFISDDGLIRETVKKFLG"
     gene            complement(2609..3100)
                     /locus_tag="PKU88_12285"
     CDS             complement(2609..3100)
                     /locus_tag="PKU88_12285"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HPX70089.1"
                     /translation="MYKINCESMGDVKKYIWYYRKDNSEHGPYTYEDIVEMVREGVIR
                     PEDYVLKFGNRKFIKVSEMQGLSEIQGPPEMQKLPEMQEPSEAGEKPEETKEEQHEGM
                     KEAAPEIKKEKAKEEHHIAHDNRAKYIQGKHKKESFSPKLPVIIACFVGLCFIVVLWI
                     LLS"
BASE COUNT          898 a          904 c          602 g         1146 t
ORIGIN      
        1 gccaatgtaa tcatcgtaaa cagcactaca tttacgaatt tacgggtaaa cccctgtttc
       61 atatagtgca tatatactat tgcggctcct cccccccaca gcaaatgaaa taagggggcc
      121 ccgaaaatat tgaacagcgg tttgttgtac tggtataagc caagggaaac aatatagaag
      181 tcaaccgtac tcaggacaat aaaccccaat acggctacag gaatgagttc cttgatgcgt
      241 tttggcttaa tgaatatgaa cacataaatc caagtggcca aaaaatacaa caaatgcgtg
      301 taccttaaaa attccatact ctcacctcac aaatattgtg aggtactttt gcataaacta
      361 tacagtaagc tgatagtgta aaaaacaaca cagaaaagct gctgcaaaac gagagtctgc
      421 agcagctcct ttttacggtt tgttatccca aaaacttttt cacagtttct ctaatcaagc
      481 catcgtcaga tatgaagggc agtgttatat gatccttccc ttcctgagat ttattgtatt
      541 caatacttgt tgttacggaa ttctcatttc ttgcccagga ccttcttgca accccgccca
      601 taacatccca aagcatcgcg tcctttatta tttcatcaac acgttcgctt ccgtcaagca
      661 caagtccgaa gcctccgttt atcgcctttc ctataccgac tccgccgccg ttgtgaagag
      721 cgaccaggct catacctctt gcggcgtttc ctgcaaaaca ctgcaccgcc atgtccgcca
      781 ttatattgct tccgtccttt atattggaag tctccctgaa gggggagtcg gttccgctta
      841 catcatgatg gtcacgtccc agcattacag gccctatttc cccttttctg accatttcat
      901 taaatttaag cgctatcttg gtccttcccc atgcatcctg ataaaggatt cttgcctggg
      961 ttccgacaac cagcttgttc ttttcggcat ctcttatcca tacccaattg tccctgtcct
     1021 gggcttttct tgcagggtca atacactcca tcgctgcgtg atccgtcttt atcagatcct
     1081 catgctttcc gctcaggcat acccacctga aaggcccata gccgtaatca aaaagttcgg
     1141 ggcccatgat atcctcaaca tatgaaggga atatgaatcc gtctttttca tcaacaccgt
     1201 tctttgctat ttcctttgct cctgcatcat acaccgcttt catgaaggaa ttgccgtaat
     1261 cgaagaaata tgtcccttta tccgccagct tccttataag ctggaagtga tgcctcaagc
     1321 tcttatccac caatttgcag aactgctccc tgtcttcttt aagcatcctg gttctttctt
     1381 caaaggtcag tccctgggga cagtaaccgc cgtcatatgc ggcatggcat gaggtctggt
     1441 ccgacagaag gtgaatcttt atatcattgt taactgcata ttcaagcaaa tctactatat
     1501 ttccatgata tgcaattgat attttatctt ttttcttcag gtaatcctct gctatgctga
     1561 atacttcctt aagatcagag gaaactctgg atatccaacc ttgattaagc cttgtttcta
     1621 ttcttgaata gtcaacctca gcaactattc ctactccgtt tgctatctcc gccgcctttg
     1681 gctgagcgcc gctcatgcct ccaagccccg aggttacgaa aagataacct ctcaaatcct
     1741 cttcgggtgc tatgccgagc ttttttcttc ccgcattaag tattgtgttg aaggttccgt
     1801 gcactatccc ctgtggcccg atgtacatcc agccccctgc tgtcatctgc ccgtagtttg
     1861 caactcccat ctgcattgca tagtgccagt ccttctggtt gtcaaacatt cctaccatta
     1921 gtgcattggt aatgaccacc cttggatccg taggctttga atgaaacaac cctaagggat
     1981 gccctgattc cacaacaagg gtctgatgat cggtcattac ttcaagatat ttctttatca
     2041 gcctgtactg catccagttc tggcatacct gccccgtttc tccgtaggtt accagctcat
     2101 aaggatataa ggctacttca aagtccaggt tattgtctat cattacttga aaagcctttc
     2161 cttctatgca gtttcccttg tattcgtcta taggctttcc gtaaatcctg ccttccggcc
     2221 tgaaacgata accatagatt cttccccttg taacaagctc ttccataaac tcgggagcaa
     2281 gcttttcatg aagctcctca ggtatatatc tgagggcatt tttcaatgca atctccgcct
     2341 gatcaggagt caatgtaaag cctctgttcg gtgcccttct gatgccttct gcaaacttgg
     2401 gcatatccgg cagccggttg tccagcttaa ctgtcattgc ctctgaaatc tcataattct
     2461 taaccaattt aattccccca atgcctgatt atactattat tctatcttat tatagtctat
     2521 taattgcaca aaatattaaa ttcagtatag taattattcc gcttcttttt tcagaataaa
     2581 aaaccttgaa attcaaggtt tgaaaaaatc aagacagaag tatccacaaa actacaataa
     2641 agcataagcc tacaaaacaa gctataatta ccggtaattt tggactgaag gattctttct
     2701 tgtgtttccc ctgaatatac ttggctctat tgtcatgtgc aatatggtgc tcctcttttg
     2761 ctttctcttt ctttatttca ggagccgctt ccttcattcc ctcatgctgc tcttccttcg
     2821 tctcttcagg tttctcaccg gcctcagaag gttcctgcat ctcaggaagc ttttgcattt
     2881 cgggaggccc ttgtatctca gaaagccctt gcatttcaga taccttgatg aacttcctgt
     2941 taccaaactt cagcacatag tcctcaggcc tgattacccc ttctctgacc atctcaacaa
     3001 tatcctcgta ggtataaggc ccatgctcgc tattgtcctt tctgtaatac catatgtact
     3061 tttttacatc ccccatgctt tcacagttga ttttatacat tagcttcacc tgccaaactt
     3121 aacattttgt atactccagt taaattatac cattaataaa caaattctga aatcttttgt
     3181 tttaataata tcacaaattt cgactattat gataccgcca aaaataaaac cgacggtgtc
     3241 aatttgttct cggtttttaa aaagcagatt ctccctttat ggtttaacta aatcagtatt
     3301 ccgggtaaac gcacaagttg ccttgcatcc gttctttgag attttctcag atgtcagcat
     3361 tacacaaaga aaaatattat tattgtaccg cttgccattt tgtcaaggct aaaatgaacg
     3421 gtgctccgca cgcggccttg acaagaagcc tggctccgtc ggtcgcggtg catatattat
     3481 gctttttttt tctctttttt cttctctttt tttgtattta ttttggtttg ctttcctgct
     3541 gtttagatta
//
LOCUS       DAOOAB010000096         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_33211_length_3222_cov_18.241869, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000096 DAOOAB010000000
VERSION     DAOOAB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33211_length_3222_cov_18.241869"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /gene="floA"
                     /locus_tag="PKU88_12290"
     CDS             complement(<1..699)
                     /gene="floA"
                     /locus_tag="PKU88_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052352.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPX70090.1"
                     /translation="MSDLLFYVVSLFVIILGLTIFLSFVPLGLWISSLAAGVNVGIFN
                     LVGMRLRRVIPSRIVNPLIKARKAGLDVGVNQLEAHYLAGGNVDRVVNALIAAQRAEI
                     ELGFERAAAIDLAGRDVLQAVQMSVNPKVIETPVVAAVAKDGIEVRAKARVTVRANID
                     RLVGGAGEETIIARVGEGIVTTVGSSESHKSVLENPDLISRTVLAKGLDSGTAFEILS
                     IDIADVDVGRNIGAQ"
     gene            complement(722..1204)
                     /locus_tag="PKU88_12295"
     CDS             complement(722..1204)
                     /locus_tag="PKU88_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPX70091.1"
                     /translation="MFGDWGLLTAFLYVTGMVLLMIEALMPGFGVAGISGIVLVLASI
                     VMISSSFFQAILLLVGTTAIAVILVVALYRMGYGKRFVKSMILNTEQKNEEGYVSTKG
                     YEKYLGMRGTVATPLRSAGTVIIDGNKIDAVSEAEFIDKGIEVEVIKIEGGRIVVKRI
                     "
     gene            complement(1430..3109)
                     /locus_tag="PKU88_12300"
     CDS             complement(1430..3109)
                     /locus_tag="PKU88_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPX70092.1"
                     /translation="MNENTNESSNFIHDFIDIDLEEKVYDHVHTRFPPEPNGYLHIGH
                     CKAMYINFSTAEKYRGLCNLRFDDTNPIKEDVEYVQSIMEDIKWLGFDWEDRLYYASD
                     YFEKLYEFAEHLIKKGKAYVCDLSQQEVREYRGTPTEPGKNSPFRDRTVEENLDLFRR
                     MRAGEFPEGSRTLRAKIDMASPNLNMRDPVIYRILKSSHHRTGDKWCIYPMYDFQHPL
                     SDALEGITHSLCSLEYEDHRPLYNWVINECEPEHKPRQIEFARLNLTYTVMSKRYLRE
                     LVETGIVSGWDDPRMPTLCGLRRRGYTADALRDFCDRIGVAKTNSTVDYALLEHCIRE
                     DLNKKADRVMAVLRPLKVIIDNYPDNQIEYFEVENNPENPDSGTRKVPFLREIYIEQE
                     DFMENPPRKYFRLYPGGEVRLKGAYFIKCESVVKDPLTGDIKEIHCTYDSASKGGNSP
                     DGRKVKGTIHWISAEHAIDAEVRLYDQLFAVPNPGEEGHEYKDDLNTDSLEVLTGCKV
                     EPVLREASAGKGYQFLRMGYFCSDNKDFTKERPVFNRIVGLKDSWAKIVKE"
BASE COUNT          845 a          845 c          569 g          963 t
ORIGIN      
        1 ctgggcgccg atattcctgc ctacatctac gtcggctata tctattgaaa gtatttcaaa
       61 ggctgtaccg gaatcaagcc cctttgccag aaccgtcctt gatatcagat ccgggttttc
      121 caacactgat ttgtggctct cggagcttcc gacggttgta acgatacctt caccaactct
      181 ggcaatgata gtctcttcgc cggcacctcc taccagtctg tcgatattgg ctctcacagt
      241 cactcttgct tttgccctta cttctatacc gtccttagct actgctgcta ccacaggagt
      301 ctcaattacc ttcggattaa cgctcatctg cacagcctgc aaaacatctc tccccgccaa
      361 gtcaatagct gccgcccttt cgaagcccag ttctatttcc gctctctgtg cagctataag
      421 tgcatttacc accctgtcaa cattacctcc cgcaagatag tgggcttcaa gctggtttac
      481 accaacgtca aggccggcct ttctggcctt tataagaggg ttcacaatcc tgcttggaat
      541 aaccctcctg agcctcatgc ccacaagatt gaaaatcccg acatttactc ctgccgccag
      601 ggaagaaatc cataatccca ggggaacaaa acttaaaaat attgtcaagc caagtattat
      661 tacaaacagg gatactacat aaaataataa gtcactcatc gatatacctc cgattcttta
      721 atcatattct ctttaccact attctgccgc cttcaatctt tataacctca acctcaatac
      781 ccttgtcaat aaactctgct tcggacactg catcaatctt gtttccgtca attatcacgg
      841 tacctgcaga tcttaacgga gtagcaacag tccctcgcat gcccaaatat ttttcataac
      901 cctttgtact tacatatcct tcttcatttt tctgttcagt attcaatatc attgatttta
      961 cgaagcgttt cccgtaaccc atcctgtata gggcaacaac cagtataaca gctattgctg
     1021 tggttcctac aagcaaaagt atggcttgaa agaagctgga tgaaatcatt actatactgg
     1081 ccaatacaag tacaataccg cttattcctg caacaccaaa ccccggcata agtgcctcaa
     1141 tcattagaag caccataccc gtaacgtata ggaatgccgt aagcaggccc caatctccaa
     1201 acatccgttt cacccccaac attcaaatat tatatttatc cagtatattg tataaaatat
     1261 agaattattt atgccctgta ttatttaagt ataatattat cataacagtt ttatgcaggg
     1321 ttaacaactg gaaataagtt aagtggggac acggggacgt ttctcctgtc ccgctttcag
     1381 cgggacagga gaaacgtccc cgtgtcccat atcccaaaat ccttaaatgt tattctttta
     1441 caatctttgc ccaggaatcc ttcaacccta ctattctgtt gaagacaggc ctttccttcg
     1501 taaagtcctt attatcagaa cagaaataac ccatcctcag gaactgatag ccttttcctg
     1561 cagaggcttc cctcaatact ggttcaacct tacagccggt caacacttcc agtgaatcgg
     1621 tgttcagatc atccttgtat tcatgcccct cttccccggg attcggcact gcaaacaact
     1681 ggtcatacag acggacttca gcatctatcg catgttcggc agatatccaa tgaatagtac
     1741 ccttcacctt acgtccgtcg ggggagttcc ctcccttgga tgccgaatca taggtacagt
     1801 ggatttcttt gatatcacct gtcagcggat cctttacaac actttcacac tttatgaaat
     1861 aagccccttt cagcctgacc tctcctcccg gataaagcct gaagtatttc cttggcggat
     1921 tctccataaa atcctcctgt tcgatatata tctcacgcag gaagggcacc ttgcgggtac
     1981 cgctgtcagg attctcagga ttgttctcca cttcaaaata ctctatctga ttatccggat
     2041 aattatcgat aataaccttc aaagggcgaa gcaccgccat aacacggtct gcctttttat
     2101 tcaggtcctc ccttatgcaa tgttcgagaa gtgcataatc tacggtgctg tttgtctttg
     2161 caacgccaat cctgtcacag aagtccctga gtgcatcggc agtatagcct ctccttctca
     2221 aaccgcataa agtaggcatc ctggggtcat cccaaccgct gactattccg gtttccacca
     2281 gttccctcag gtatcgtttg ctcataacgg tataggtcag gttcagcctg gcaaattcta
     2341 tctgccttgg cttatgctca ggttcacatt cattaataac ccaattgtac agaggccggt
     2401 ggtcctcata ttccaatgaa cagagagaat gggtaattcc ttccagagca tcagataacg
     2461 gatgctgaaa atcatacatc ggatagatgc accatttgtc tcccgttctg tggtggcttg
     2521 atttcagtat tcggtatata acagggtccc tcatgttcag atttggtgat gccatatcga
     2581 tcttggcacg aagcgttctt gaaccttcgg gaaattcacc tgccctcatt ctcctgaaca
     2641 aatccaggtt ctcctcaaca gtcctatccc tgaaggggct gtttttaccc ggctccgtag
     2701 gcgttcctct atattctctt acttcctgct ggcttaaatc acatacataa gctttaccct
     2761 tctttataag atgttctgca aattcataaa gcttttcaaa atagtcggac gcatagtaaa
     2821 ggcgatcctc ccagtcaaag cccagccatt tgatatcctc cataatggat tgcacatatt
     2881 caacatcttc cttaatggga ttggtgtcat caaaccttaa attgcataat ccgcgatatt
     2941 tttccgccgt actgaaattt atgtacatag ccttgcaatg gcctatatgg agataaccgt
     3001 tgggctccgg aggaaaacgg gtatgtacat ggtcatatac tttttcctcc agatctatat
     3061 caataaaatc gtgtataaag tttgaactct cattggtatt ttcgttcaag gacattacct
     3121 cctttttttt gcgcaataca ttagtatgct gaatattata ctatatagat tctgcttttt
     3181 ccagtaatct tatgaatatt tggagacggg cgccatgtat gg
//
LOCUS       DAOOAB010000097         2965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_36973_length_2965_cov_13.614777, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000097 DAOOAB010000000
VERSION     DAOOAB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36973_length_2965_cov_13.614777"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="mnmA"
                     /locus_tag="PKU88_12305"
     CDS             complement(<1..803)
                     /gene="mnmA"
                     /locus_tag="PKU88_12305"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038016936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPX70093.1"
                     /translation="MQMKKRVVIGMSGGVDSSVAAYLLKQQGYDVIGVTMQIWQDKSK
                     EALEREGGCCSLSAVEDARRVCAKIDIPFYVMNFKEVFEKLVIDYFVDEYLCGRTPNP
                     CIACNKFIKFDALLERSRALEADYVATGHYARIQYEENLKRYVIRKSAAPDKDQTYVL
                     YNLTQEQLRHILMPLGDYNKSQVREIARELDLRTANKPESQEICFVEDNDYGRFISER
                     KGSEIKPGYFVDTQGRMLGTHKGIVHYTVGQRKGLGIAFGKPMYVVEIIP"
     gene            complement(828..1277)
                     /gene="nifU"
                     /locus_tag="PKU88_12310"
     CDS             complement(828..1277)
                     /gene="nifU"
                     /locus_tag="PKU88_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPX70094.1"
                     /translation="MYSEKVMDHFSNPRNVGEIEDASGVGEVGNAKCGDIMKIYLKIE
                     DNIIKDVKFKTFGCGAAIATSSIATEMIKGRSVEEALELTNKAVVEALDGLPPAKIHC
                     SVLAEEAVKAAIEDYMKKQGKEVESSVSAGIEPDEEACCSKCERKCH"
     gene            complement(1270..2463)
                     /gene="nifS"
                     /locus_tag="PKU88_12315"
     CDS             complement(1270..2463)
                     /gene="nifS"
                     /locus_tag="PKU88_12315"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPX70095.1"
                     /translation="MDGIRGIYMDHAATTYTKREVLEEMLPFFTEYFGNPSSIHKFGR
                     DVRKHVDTARERVAKALGALPEEIYFTAGGSEADNWAVKGTAYANKSKGNHIITTNIE
                     HHALLHTCEYLEKEGFEVTYLPVDEYGLVSVEDVKNAITDKTILISIMYANNEIGTIQ
                     PIEEIGKLAREKGIYFHTDAVQAVGSVKIDVKEQNIDMLSLSAHKFYGPKGIGVLYVR
                     KGVKLINLVHGGGQERGRRAGTENVPGIIGLGKAIELAAENLEESSKRIMGLRDKLIK
                     GVMNKIPYTRLNGHPVKRLPGNANFSFQYIEGESLLLNLDMKGIAGSSGSACTSGSLD
                     PSHVLLAIGLSHEIAHGSLRLSLGAVNTEEDIDYVLEVLPEIVERLRQMSPLYEKVKG
                     GNTNV"
     gene            complement(2465..2905)
                     /locus_tag="PKU88_12320"
     CDS             complement(2465..2905)
                     /locus_tag="PKU88_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPX70096.1"
                     /translation="MILSTKGRYGLKIMYELALNYGEGPMPLKEIAQRQQLSETYLEQ
                     LIAHLKKAGLVRSIRGAQGGYELIRKPEEISVGEIIRTLEGPLAPSECVMDDEPECSK
                     AENCVTRLIWEKIMEGINNVVDSITLNDMVNDYYKLKRKKEGEK"
BASE COUNT          697 a          788 c          509 g          971 t
ORIGIN      
        1 ggaattattt cgactacata cataggcttt ccaaaggcta ttcccaagcc cttgcgctga
       61 ccaaccgtgt aatggactat tcccttatga gtaccgagca ttcttccttg cgtatctaca
      121 aaatatcccg gcttgatttc gcttcctttt ctttcgctta tgaaccttcc gtagtcatta
      181 tcttcaacaa aacagatttc ctggctttca ggcttgttgg ccgttctaag gtccaattcc
      241 ctggctatct ccctgacctg actcttgttg tagtccccca ggggcataag tatatgcctg
      301 agctgttcct gagtcagatt ataaagcaca tatgtctggt ctttatcggg agctgcggat
      361 tttctgataa cgtacctctt cagattttct tcgtactgta ttcttgcata atgcccagtt
      421 gcaacgtaat ctgcctccaa agccctggag cgctccaaaa gcgcatcgaa ttttatgaat
      481 ttgttgcagg ctatgcacgg attcggagtc cttccgcaca gatattcatc aacaaaataa
      541 tcaataacca gcttttcaaa tacttcttta aagttcataa cataaaaggg tatgtcaatt
      601 ttagcgcata cccttctggc atcctccaca gcagataaag aacagcagcc tccttcacgc
      661 tcaagggctt ccttgctctt atcctgccat atttgcatgg taactccaat tacatcatat
      721 ccctgctgtt tcagaaggta agcggcgaca gagctgtcaa ctccgccgct catacctatt
      781 acaactcgct ttttcatttg cattcaactc ctaatgagca tacagggcta atgacacttc
      841 cgctcacatt tactgcagca tgcttcttca tcaggctcaa tcccggctga tactgaggat
      901 tccacttcct taccctgctt tttcatgtaa tcctcaattg cagctttaac ggcttcttca
      961 gccagcactg agcagtgtat ttttgcggga ggcagtccgt caagagcctc aactacagct
     1021 ttatttgtaa gttcaagggc ttcctctacc gacctgccct ttatcatttc agtggcaatg
     1081 ctgcttgtgg ctattgcggc accgcatcca aaggtcttga acttaacgtc ctttatgatg
     1141 ttatcttcta tcttcagata aatcttcatt atatcgccgc acttggcatt gcctacttcg
     1201 cctacaccgc tggcatcctc tatttcaccg acatttctcg gatttgaaaa atgatccatc
     1261 actttttcac tatacattag tatttccccc cttaactttc tcatacaacg gagacatttg
     1321 cctcagcctt tccactattt ccggcaatac ttccaggaca taatctatgt cctcttcggt
     1381 atttacggca cccagggaca atctcagcga tccatgggct atttcatggg acagaccgat
     1441 agccagcagc acatgggaag gatccaatga tcccgaagta catgccgagc cgctggaacc
     1501 ggcaatacct ttcatatcca gattcagaag cagtgactct ccttcaatat actgaaaact
     1561 gaaattggcg ttccccggaa gccttttgac cggatgtccg ttcaaccttg tataaggtat
     1621 cttattcatt acgcccttta tcaatttatc cctcagcccc attatccttt tgctgctctc
     1681 ttccaggttt tcggcggcaa gctcaatggc ctttccgagt cctataatac ccggaacatt
     1741 ttccgtacct gctcttcttc ctctttcctg accgccccca tgaacaaggt ttataagctt
     1801 tactcccttt ctcacataaa gtacccctat acctttaggg ccatagaatt tatgtgcgga
     1861 aagcgagagc atgtcaatgt tctgttcctt tacatcaatt ttaacactcc ctaccgcctg
     1921 cactgcatcg gtatggaagt atatgccctt ttcccttgcc agctttccta tctcttctat
     1981 gggttgtatc gtaccgattt cattattggc atacataatg gaaatcagta ttgtcttatc
     2041 agttattgca ttttttacat cctcaacgct taccaaacca tactcatcta caggcaggta
     2101 tgttacttca aagccttcct tttccaggta ttcacaagta tgcaaaaggg cgtggtgttc
     2161 aatgtttgta gttattatat gattgccttt gcttttgttg gcatatgccg tgccttttac
     2221 cgcccagttg tctgcttcag agccccctgc agtaaaatat atttcttccg gaagtgcacc
     2281 taatgccttg gcaacccttt ccctggcagt atccacatgc ttgcgcacat ctcgtccgaa
     2341 cttgtgtatg cttgaaggat tcccaaagta ctcggtaaag aaaggaagca tttcttccag
     2401 tacttctctt tttgtatagg ttgtagctgc atgatccatg taaattcctc ttatgccgtc
     2461 catattattt ctctccttct ttctttcgtt taagcttata atagtcattc accatatcat
     2521 tcaatgttat tgaatctacc acattattga tgccttccat tattttttcc caaatcaatc
     2581 ttgttacaca gttttcagct tttgagcact ccggttcatc atccattaca cattccgacg
     2641 gggccagggg gccttcaaga gtccttatta tttctcccac tgaaatctct tcgggttttc
     2701 ttatcagctc atatccccct tgagcacctc tgatgcttct aaccaggccg gcctttttca
     2761 gatgggctat aagctgttcc agataggttt cagaaagctg ctgcctttga gctatttcct
     2821 tcaggggcat cggcccttcc ccgtagttca aagcaagctc atacataatt ttgagtccat
     2881 atcttccctt ggttgataat atcatattat cagctcctta ttaattccta cctattaagt
     2941 cggatttcaa tttaaggtta ccata
//
LOCUS       DAOOAB010000098         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_37848_length_2911_cov_17.292017, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000098 DAOOAB010000000
VERSION     DAOOAB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37848_length_2911_cov_17.292017"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1645)
                     /gene="groL"
                     /locus_tag="PKU88_12325"
     CDS             complement(17..1645)
                     /gene="groL"
                     /locus_tag="PKU88_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514589.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPX70097.1"
                     /translation="MAKQIRFAEDARRALESGVNQLANTVKITLGPKGRNVVLDKKFG
                     SPVITNDGVTIAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVAAGANPTIIKRGISKAVDTAVEELKKNSRPIESKEAIAQVASISAADETIGELI
                     AEAMEKVGKDGVITVEESNTFGTTLDVVEGMQFDRGCISLYMVTDTEKMEAVLDDPYI
                     LITDKKISNVQELLPILEQIVQQGKKLLIIAEDVEGEALSTLIVNKLRGTFTCVAVKA
                     PGFGDRRKAMLEDIAVLTNGQVISEELGLELKETKLSQLGRARQVKVQKENTIIVDGA
                     GEPKAIKDRIKQLKVQIEETTSDFDREKLQERLAKLSGGVAVIKVGAATETELKERKH
                     RIEDALAATRAAVEEGIVAGGGTALANTIPAVNALIAGADGDEKTGIRIVKRALEEPV
                     RQIAENAGLEGSVVIEKVMASKPGIGFDALKEEYTDMIQAGIVDPTKVTRSALQNAAS
                     VAALLLTTEGVVADLPEKEKPMMPGGGMGGDMMY"
     gene            complement(1663..1947)
                     /gene="groES"
                     /locus_tag="PKU88_12330"
     CDS             complement(1663..1947)
                     /gene="groES"
                     /locus_tag="PKU88_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPX70098.1"
                     /translation="MNIKPLGDRIIIKVIENEETTKSGIVLPGTAKEKPMQGEVLAVG
                     SGEMVDGKKIPLELKAGDKVIYSKYAGTEVKMDGNEYLIIRQSDVLAIIE"
     gene            complement(2210..2887)
                     /locus_tag="PKU88_12335"
     CDS             complement(2210..2887)
                     /locus_tag="PKU88_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPX70099.1"
                     /translation="MLGTIANAGAIIIGGIAGSFFRKGISERFSNIIMSVLGLFTLVL
                     GMMFAVDTQNALVVIFSLVIGSVIGEWIDIEKRMNDLGDYVQGKLNSGEGSFSKGFVT
                     ASLLFCVGTMSIMGSLQSGLMNNHNILLTKSILDGTISVVFASTLGIGVVLSSLPVLV
                     YQGSIALLASSVAPYLSEAVITEMTAVGGVLLVGMGINLLETRKLKVGNMLPAIFLPI
                     ILMLFMK"
BASE COUNT          704 a          781 c          494 g          932 t
ORIGIN      
        1 tgtcctctat ttcatcttag tacatcatgt ctccgcccat accgccgcct ggcatcattg
       61 gcttttcctt ctcaggcagg tctgccacca caccttccgt agtaagcagc aatgctgcaa
      121 cggaagccgc attctgaagt gctgatcttg taacctttgt cgggtcaact attccggcct
      181 gaatcatgtc tgtatattct tctttcaaag catcgaaacc gattccgggc ttgcttgcca
      241 taaccttttc aattaccact gagccttcaa gtcccgcatt ctcggcaatc tgtctgacag
      301 gctcttccaa tgccctcttg actatcctga tgcctgtttt ttcgtccccg tccgctccgg
      361 caatcaatgc gtttacagca ggtattgtat ttgcaagtgc agttccgccc cctgctacta
      421 taccttcttc aactgccgcc cttgttgctg caagagcgtc ctctattctg tgctttcttt
      481 ccttaagctc tgtctctgta gcagcaccga ctttgattac cgcaacgccg ccggaaagct
      541 ttgcaagcct ttcctggagt ttttccctgt cgaagtcgga agtggtctct tctatctgta
      601 ctttaagctg tttgattctg tctttgattg cctttggttc tccggcaccg tcaacaataa
      661 ttgtattttc tttctgaacc ttgacctgtc ttgctcttcc aagctggctc agttttgttt
      721 ctttaagctc caagcccagt tcttcggata taacctggcc atttgtgagt actgcaatat
      781 cttccagcat tgcctttctt ctgtctccaa agcccggagc ctttactgcc acacaggtga
      841 aagtgcctct gagcttattg acaatcaatg ttgacaaggc ctctccctca acatcctcag
      901 caataataag aagcttctta ccttgctgaa ctatctgttc aagaattggc agcagttcct
      961 gtacgtttga aatcttcttg tcggtaatga gtatatatgg atcgtccagt acagcttcca
     1021 ttttctccgt atcggtaacc atatataatg agatgcagcc tctgtcaaac tgcatgcctt
     1081 ccaccacatc gagtgttgtt ccgaaggtat tggattcttc aacagtaata acgccatcct
     1141 tgcctacctt ttccatagct tcagcaataa gttcccctat tgtctcatct gcagcggaaa
     1201 tggaagcaac ctgtgcaata gcttctttgc tctcgatagg cctcgaattc ttcttaagct
     1261 cttctactgc cgtatcaacc gctttgctga tacctctctt tattatcgtc ggatttgctc
     1321 ctgctgcaac gttcttgagc ccttctctaa tgattgcctg cgcaagaagt gtagctgtag
     1381 tagtaccgtc ccctgccaca tcatttgtct tggtagcaac ttccttaaca agctgtgctc
     1441 ccatattttc aaagggatct tccagttcta tttccttcgc tatggtaaca ccgtcattag
     1501 taataaccgg tgagccgaat ttcttatcca gaactacatt tctgcccttt gggcctaatg
     1561 ttatttttac tgtattcgca agctggttaa caccgctttc aagtgcccgt cttgcatcct
     1621 ctgcaaatct tatctgtttt gccatctata ttccctccta atttattcaa ttattgccaa
     1681 aacatcgctc tgtctgatga tcagatactc attgccatcc atcttcactt cagttccggc
     1741 atattttgag tatataacct tatctcctgc ttttaactcc agaggaattt tcttgccgtc
     1801 caccatttct ccggaaccta ccgcaagcac ttcgccctgc atcggttttt cctttgcagt
     1861 acccggaagc actataccgc tcttggttgt ttcttcattt tcaatgactt tgatgatgat
     1921 tctgtcgcct aagggtttga tattcatgca tactcctcct taaagtaatt actttaaaca
     1981 ctatgtattt ttattattag cactcaccta gactgagtgc taattacact ttatataata
     2041 attaaaggaa attatattat caaactccca aataagccaa aaacagcaaa taatgataat
     2101 attcctccaa tgccttatat tatttcaaca ttacgccgta tttataccat tttcttaata
     2161 tttcaaggca taagggtcca tcgtatcccg tatctccagc cccgcggctt tacttcataa
     2221 ataacatcag tattatcgga aggaatatcg ccggcagcat attgccgact ttcagcttcc
     2281 ttgtttccag caaatttatg cccattccca caagcaggac tcctcctacg gctgtcattt
     2341 cagtaatcac tgcttcactg aggtatgggg caacggatga cgcaagcaaa gctatggagc
     2401 cctgatacac caaaacgggc agggacgaca gtacaacccc tattccaaga gttgatgcaa
     2461 aaacaacaga tatcgttccg tccaatatag acttcgtcag cagtatattg tgattattca
     2521 ttaatccgct ctgcagcgat cccataattg acattgttcc tacgcagaat agaaggctgg
     2581 cagttacaaa gccttttgaa aagcttcctt ctccggaatt aagcttgccc tggacgtaat
     2641 cgcctaaatc gttcattctc ttttctatat cgatccattc tccgattacc gacccgataa
     2701 cgagactgaa tattacaacc aatgcatttt gcgtatcaac cgcaaacatc atgccgagca
     2761 ccagagtaaa aagccccaga accgacatga ttatattgct gaaacgctca gatatacctt
     2821 ttctgaaaaa gcttcccgca ataccgccta taattattgc tcccgcattt gctatagtgc
     2881 ctaacaaaat caatatacga ccctcccaaa t
//
LOCUS       DAOOAB010000099         2695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_4081
            NODE_41900_length_2695_cov_12.954545, whole genome shotgun
            sequence.
ACCESSION   DAOOAB010000099 DAOOAB010000000
VERSION     DAOOAB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866052
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:50:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,470
            CDSs (total)                      :: 2,415
            Genes (coding)                    :: 2,392
            CDSs (with protein)               :: 2,392
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 2 of 23
            Pseudo Genes (incomplete)         :: 18 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41900_length_2695_cov_12.954545"
                     /isolate="AMR_MDS_4081"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1063
                     /locus_tag="PKU88_12340"
     CDS             65..1063
                     /locus_tag="PKU88_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPX70100.1"
                     /translation="MSYLLKPLKNGKLLLKNRLIMPPMATAKCEKDGKPSKALFDYYD
                     EKSRGGYISLIIVEHSYISQQGKASDRQLSAADDSLIDSLKELAGIIHKNGTKAVMQI
                     NHSGSAAKYEVTGMDAVAPSAVSNPRHNGPLPKELTLKEIKAIIEEFRAAAYRVKMAG
                     FDGVEIHSAHGYFLNQFLSPLANKRTDEYGGNIHGRIKIHLEVIKAVREAVGEDFPIF
                     LRLGASDYMEGGTSIEDSKIAAMEFEKSGVDIIDISGGFCGYIGRGTAEQGYFSELSE
                     AIKKVVSVPVILTGGVTEAQAAEKLLADGKADLIGVGRAILNDSDWAKKAVESLKP"
     gene            complement(1133..1771)
                     /locus_tag="PKU88_12345"
     CDS             complement(1133..1771)
                     /locus_tag="PKU88_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPX70101.1"
                     /translation="MYIRNRMTPNPCTIPSNTTIADAVALFREKGFKRFPVVDNGKLV
                     GILTKGDIQSVSPTKATSLSIYEVNYFLSKITVRKAMTKKVITIDADCLLEEAAVAMR
                     ENKISTLPVMENGKLVGIITESDIFDAFIDLMGFKEKGSRITVEVKDVPGGIAAISEV
                     FSSLHINITHIAMFAGKGIRDLIIRSSVEDVSEIEKKLNEKGYKIKHLLINK"
     gene            2262..2639
                     /locus_tag="PKU88_12350"
     CDS             2262..2639
                     /locus_tag="PKU88_12350"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HPX70102.1"
                     /translation="MQLKTILLTALGFVFLGLGAIGILLPVWPTTPFVLVSVACFSSA
                     PRIKARIVKITFFREHIENYEHRTGLSRKTVSHSMIWLWGMLLLSMAIIRTFWIIILL
                     LVVGVAVTSHILCMAKAKEGKTE"
BASE COUNT          783 a          533 c          531 g          848 t
ORIGIN      
        1 aaaatatata attaatataa aaggcaatat ttattaaata ttaaaccatt aggagggaaa
       61 tataatgtct tacttgctga agcccctgaa aaacggaaaa ttgctcttga agaacagatt
      121 aattatgcct cccatggcta ctgccaaatg tgagaaggac ggaaaaccaa gcaaggctct
      181 ttttgactat tatgatgaga agtcccgagg gggatatatc tccttgataa ttgttgagca
      241 cagctatata tcacagcaag gtaaagccag cgacaggcag ctttctgccg cggatgacag
      301 cctgattgac agcttgaagg agctggccgg tataattcac aaaaacggta ctaaagccgt
      361 aatgcagata aaccattccg gaagtgctgc gaagtatgag gttacaggta tggatgcggt
      421 tgctccttca gcagtatcca atccgcgtca caacggcccg ctgccgaagg agcttaccct
      481 taaagaaata aaagcaataa ttgaagaatt cagagctgct gcatatcggg taaaaatggc
      541 aggttttgac ggagtggaaa tacattccgc ccacggatac ttcctgaatc aatttctgtc
      601 tcctcttgcc aacaagagaa ctgatgaata tgggggaaac atacacggca gaataaagat
      661 tcacttggaa gtcattaagg cagtaaggga agctgttggt gaggatttcc cgatttttct
      721 gcgcctgggc gcctctgatt atatggaagg cggaacctcc atcgaagaca gcaaaatcgc
      781 ggcaatggaa tttgaaaaat caggagtgga tataattgat atttccggcg gcttttgcgg
      841 atatatcgga cgtggtactg ccgagcaagg ttatttttcc gagctttctg aggcaatcaa
      901 aaaggttgta tctgttcctg tcattttaac cggcggagtc acagaagccc aagctgctga
      961 aaagctgctt gcagacggaa aggctgattt gattggagta ggaagagcta ttcttaatga
     1021 ttccgattgg gcaaaaaaag ctgttgaatc actgaagcca taaagaaaaa tgccaggcac
     1081 catacgtatc tgtccttctc gataggcgat aatgtcggtg cctggctcat ttttatttgt
     1141 tgattaacaa atgttttatt ttatatcctt tttcattaag ctttttctct atttcagaaa
     1201 catcctccac cgatgaccta attattaaat ccctgatccc tttgccggca aacattgcta
     1261 tatgggttat attaatatgc aaggaagaga atacttcgga aatagctgct atgccgccgg
     1321 gtacatcttt tacttcaacg gttatacggc ttcctttttc tttaaagccc attaagtcta
     1381 tgaaagcatc aaatatatcg ctttctgtta ttattcccac caattttcca ttttccatga
     1441 ccggaagggt gctgattttg ttttctctca ttgccactgc cgcttcttcc aataaacagt
     1501 cagcatctat agtgattact ttttttgtca ttgcttttct taccgttatt tttgacagaa
     1561 aataattgac ttcatagata ctcaaagatg tggcttttgt tggggatact gactgaatat
     1621 caccttttgt aagaataccc accagtttcc cgttgtctac tacgggaaat cttttgaaac
     1681 ccttttctct gaaaagtgcc accgcgtctg caatagtagt gttggatggt attgtacacg
     1741 gattcggggt catcctattt ctaatataca tactattctc ctttcgctta tactattttt
     1801 ccgcttcatt attattgtga tacccttcaa tatcgttaca ccaatgaata tatttctctc
     1861 tgccctttat attacaatta tatcaaatga caataccgtg agtaaagagt cataattcac
     1921 aagagaccct ccttggtgtt tattttgttt tgcaactaat attttaccaa aaagagtggt
     1981 ctcttgctat tttttgacct acaacaattt tacactaagc atgctttatc gttttccata
     2041 aatagaaaaa gtcccgcagg atggcactgt aaaagccacc ttgcattcta agccaccatt
     2101 ctgattacaa tggatgctaa gtcaagtaca gaaaagtttg taaattcttt cctcttataa
     2161 gaagttttac ttcgaatcgt ttttcatttc ctgttgaaaa aattcaatca tatgatataa
     2221 tagtattaag tcctattaac aataataagc gtggtgatga tttgcaactg aaaacaattt
     2281 tacttacagc acttgggttt gtatttctgg ggttaggcgc aattggtata ttattgcctg
     2341 tctggccgac aacccctttt gttttagttt ctgtcgcatg tttttcttca gcgcctcgca
     2401 ttaaagcccg tattgtgaaa attacatttt tcagggagca tatcgaaaac tacgaacata
     2461 gaacgggtct atcacgtaaa actgtcagcc atagtatgat ttggttatgg ggaatgctgc
     2521 ttctttctat ggcaatcatt cgaacctttt ggataataat acttcttctc gttgtcggtg
     2581 ttgctgttac aagccacatc ctttgtatgg ctaaagccaa ggaaggcaag acagaatgaa
     2641 acgtgtattt ggaactgtcg aagcaatatt cgatctatta tacctaaccg cagca
//
